LOCUS       DAOMUE010000001        42779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_66_length_42779_cov_2.534337, whole genome shotgun sequence.
ACCESSION   DAOMUE010000001 DAOMUE010000000
VERSION     DAOMUE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 42779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42779
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66_length_42779_cov_2.534337"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PLC71_00005"
     CDS             complement(<1..692)
                     /locus_tag="PLC71_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRM01060.1"
                     /translation="MLIERYNEVKKHVEEACKRVGRDPREVTVIAVSKTKPLEMVEEL
                     RKEKVLDFGENKVQEIRDKYANISWPVRWHLIGHLQTNKIKYIVDKVALIHSVDSIKL
                     AQAIDKEAAKHDVKEVPILIQVNFAHEDTKFGLNPEDVFDVVKEISALEHVKIKGLMQ
                     IAPFVDDPEDNRKYFRAMKQLSVDINNKNFDNVDMSILSMGMTNDYEVAIEEGATMVR
                     VGTGIFGARDYSR"
     assembly_gap    760..769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<770..>1233)
                     /locus_tag="PLC71_00010"
     CDS             complement(<770..>1233)
                     /locus_tag="PLC71_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01061.1"
                     /translation="RYINERYLEIDVTLDSYKGLKIPNTSIVKKKFYQVPVKYLTKGD
                     NSAKEQFTVRDTSNKGDVTVEQKSFTIYGRTKDYCYLDPEEVGENVVLQAMDSKDTFL
                     IEKMKTLKGVYCTNQGYADFRPIDILIEKDDYSIIANDTNQGVSRYDFIVLDG"
     assembly_gap    1234..1243
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1244..2116)
                     /locus_tag="PLC71_00015"
     CDS             complement(<1244..2116)
                     /locus_tag="PLC71_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRM01062.1"
                     /translation="MAAKRRRTTKIHWNIGMIIFVVILVYILINVILFLGKKKLTVYK
                     VTEDKITNTLSFTGIAIRDEELLKASQSGYITYYVEEGKRIKKSGTVFTVDKDQKVQD
                     AFVSQVQQLEKNKKVIDDEEIQHKITDYQSVYSDHKFSTVYDLKYDLKNAILNLSEDS
                     MKTVIEAVKQKLGDTSFETQKSTSSGVTQFYSDDFDGKSAKDITSKDFDQSQYRVQRY
                     NTSDKVKKGKVVARINKSEKWQIVIPLEADQYRMLKDKSEVSVRFLQDQTTATATVEV
                     TKKGSSYFGYLKFND"
     gene            complement(2120..3061)
                     /locus_tag="PLC71_00020"
     CDS             complement(2120..3061)
                     /locus_tag="PLC71_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202997.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRM01063.1"
                     /translation="MERYKAYSTHLKELYGEKVYKLPVNLPVTCPNRMDGDGCTFCGG
                     VGTGFEAMNSEVSVSEQLNATKGKITKRYKAKKFIAYFQNYTNTFLPVDKFEKYLVEA
                     AQTEDIVGISVSTRPDCITKEYLDCLKKISEEYQVKISMEYGLQTVNYKTLNKINRGH
                     TLAEYLDAVLMTAPYGFEICTHIILNLPGDDMTDVIETAKILSALPVQIVKLHSLYIP
                     KGSKMYEEYKEGKITLCDPKEYLDRLVNFICYVRKDMVIERLFSRVPKEDASFSNWGI
                     SWWKLKDRFDEIMETNDYMQGCKFDYLNGAALRRWEI"
     gene            complement(3157..4026)
                     /locus_tag="PLC71_00025"
     CDS             complement(3157..4026)
                     /locus_tag="PLC71_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRM01064.1"
                     /translation="MKKLICICMSVMMIALSGCQTSAKTGGFTSYDEHYQKNETTLKE
                     DALSDRWESRGSKVAVVSKEKADNNDYPEAKEVLLVNNTKNKVIVSQKVFDQAYPAST
                     TKIMTALLTLENLNLSQVVTIKHDITFPDGAAVAIHLKKGDKITVEALLNALIIMSAN
                     DAAVALGEAVAGSEANFVKMMNKRAKELGATNTHFANPNGLHLSDHYSTAYDLYLIFK
                     EVAKHNEFFNIAGRSSSRIEYLGSKGEQKIYDMASTNQYIAGTYTLPGIYDRRQDWYN
                     NTGGFLPDSSYKE"
     gene            complement(4065..4457)
                     /locus_tag="PLC71_00030"
     CDS             complement(4065..4457)
                     /locus_tag="PLC71_00030"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HRM01065.1"
                     /translation="MNVGLVAHDGKKNLMQNLCIAYQDILKKHKLYATSTTGQMIEDV
                     ANLKIHKLLAGHFGGMQQMGSKISNNEIDLLIFLQDPANKKRTPDFYNVLNLCDQYNI
                     PCATNLPTAEVLILALDRGDLDWRNMYK"
     gene            complement(4470..5585)
                     /locus_tag="PLC71_00035"
     CDS             complement(4470..5585)
                     /locus_tag="PLC71_00035"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRM01066.1"
                     /translation="MLRNYRLQNYNFKLVFNVLVLMAMSLLFIHSAKASYVPKQALGI
                     IMGLGIIVVVSLIDYQVFTRNAEILYILNVIMLIGVKLFGKDVNGAKRWFSLGPLGTF
                     QPSELSKVIMIIVVAAFLAKHQDDLNEPKVLGKLAVICGIPLLLILKQPSLSSTLDIC
                     FIILGMIFMAGLSSRLIVQVLIVGVPLLAIFLWYVQTPGQVLLEPHQVARIMSFLHPE
                     NYADSTALQTSNSIMAIGAGGLFGKGFGSNTISNVSASDVNLVSENQTDFIFSVIGEE
                     FGFVGCVVLIIVFACLVYQCMNVAKKSGNLIGTYVAVGVACYMGFQSFINIAVATGTM
                     PNTGQPLPFISYGLSSLMSASIAIGMVLNIYLQRKRH"
     gene            complement(5601..5861)
                     /gene="minE"
                     /locus_tag="PLC71_00040"
     CDS             complement(5601..5861)
                     /gene="minE"
                     /locus_tag="PLC71_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HRM01067.1"
                     /translation="MGFFDNFFKKKSSGEVAKDCLKLLLVSDRSNCSPETMELIKNDI
                     IKVISKYMEIDPEGLDIQITQSSEESEGPALYANIPIRELRK"
     gene            complement(5876..6661)
                     /gene="minD"
                     /locus_tag="PLC71_00045"
     CDS             complement(5876..6661)
                     /gene="minD"
                     /locus_tag="PLC71_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HRM01068.1"
                     /translation="MSEVIVITSGKGGVGKTTTTANVGTGLAKEGKKVVLIDTDIGLR
                     NLDVVMGLENRIVYNLVDVVEGNCRIKQAMIKDKKYPNLFLLPSAQTRDKTSVTPEQM
                     SKLVEELKSEFDYIILDCPAGIEQGFKNAIAAADRALIVTTPEVSAIRDADRIIGLLE
                     ANDIHKIDLVINRIRMDMVERGDMLSKDDVLDILAVDLIGIVPDDENIVISTNQGEPL
                     VGSNTPAGKAYQNICNRVMGKDVPFMEITGPTFFQRLANVFKK"
     gene            complement(6680..7420)
                     /gene="minC"
                     /locus_tag="PLC71_00050"
     CDS             complement(6680..7420)
                     /gene="minC"
                     /locus_tag="PLC71_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HRM01069.1"
                     /translation="MNQPVLLKGNSLGLTMVLDPGMKFDQLIKAIEDKFVQAKDFFNG
                     QTQIALKIEGRKLDAKELQNVLQIIAEKTTLTIAYVIEDDKIMETSFKDVLKKIQERE
                     QKRSQSMVDERRKGDGQFYRGTLRSGQSLESDASVVVVGDVNPGASVCAKGNVVVLGC
                     AKGYLSAGCDGDDHAFVAALDMQPMQIRIGKHIARSADGEPEKKKKLFGRSKNNDNQP
                     MEAQIAFVEDENIYIEPISKALLNEIIV"
     gene            complement(7441..10353)
                     /locus_tag="PLC71_00055"
     CDS             complement(7441..10353)
                     /locus_tag="PLC71_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM01070.1"
                     /translation="MLRKIYEKAEKLLANRLLALIILIVVLYCVLIGRVFVLQIVEGQ
                     SHKNDFTYKVQKTVKTSGTRGNIYDVNGKLLAYNKLVYTVNFQNDNAFQTLAAKNGTS
                     ESYEKNKVIYKVIKILERNGDSFINEIPIEYTGSGKFRFTETGSKLKKFKRDVFGIGN
                     STDLSKSEKELRDKQLNATAEQVFEYLRNGTLGSAGTGKMFDIDKSYSKKDALKIMSV
                     RYSAFLSRYSQYMKVTIANEINNRSIAEIKERSSELPGIDIDTKSIRVYNKSEAMSHV
                     IGYTGTVNTDELETYNKGKKEEDKDYYSSDETVGKAGVEKQFENYLHGDSGSKTLVVN
                     NVGKIIDTTKTVKSGTGNNITLSIDSELQEYVYNLLEKKIAGIVLSKLTSSDSAGNDR
                     ENIMIPIKKVYYSFIGNSVIDLENLNGDKATSYEKKMYRKIQTLEDQAINVSKNLVLK
                     DTKAYKDQSEEKQAYASYVYSLLSSKKVLISSSIDTTDKTYQKWKNEKISLSEFLRYA
                     VNKEWIDISSLNISSKYNDTEEIMKALAAYVEDALVDADDFDMTVCEQSIMKGKLSGR
                     EVCLLLYEQGVLKKKGDSDYTALKSGSLNSYDFIRRKLKSLQITPGQIGMDPCSGSVV
                     ITDSKTGKVKALVSYPGYDSNRLSNGTDSGYYRQLANSASTPLYNQALKHKTAPGSTF
                     KPVSALAGLNEKAITTSTVINCTGLYDKITPPAKCWKYPGRHGARTVSTAIEASCNYF
                     FYEVGYRLGDKSGSYSSKEGLKYLEKYATQLGLNKQSGIELDESSPQVSDETSVRSAI
                     GQGRNSFTPSQIANYVTTLSNSGTVYDLSIMDKITDDNGKTVKKYGVKKVRQLHYDTT
                     YWNAVHTGMRGVVSGKDSSVSSIFQGMKVKLAGKTGTAQENKKRPNHALFISYGPYEK
                     PEITTTVVIPFGYTSSNAAATAKDIYEYYFANDKTKKTLEKNNKSVTETSVGTAGD"
     gene            complement(10353..10868)
                     /gene="mreD"
                     /locus_tag="PLC71_00060"
     CDS             complement(10353..10868)
                     /gene="mreD"
                     /locus_tag="PLC71_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HRM01071.1"
                     /translation="MKIKRILFYVISVLVCFLLQTSVFNFLKLAGVMPNILLILTVTI
                     AFIRGRKAGIVIGFVCGLLIDIFSGNVLGQNAFVYLMFGYVNGWFHTYFYEDDILLPI
                     GLLTANSLAYSFVIFFFFFAMRGRLQFFSYLFKVMIPEAVYTGIVALLIYKILLEVDI
                     RIRDGEKRSMF"
     gene            complement(10880..11746)
                     /gene="mreC"
                     /locus_tag="PLC71_00065"
     CDS             complement(10880..11746)
                     /gene="mreC"
                     /locus_tag="PLC71_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRM01072.1"
                     /translation="MKLQKKKFNSNHLLAIFIVVCGLLAGISVFAGGVLTPVKNIFFT
                     VLSPMQEGINSVGNAVFSWNENAKSKKTLRAENERLQEKIDVLKMQNRVLQQNQVELE
                     RLQDLLKLKQKYKKYHTVGARVISKGSGNWFDIFLINRGSKDGIKKDMNVIAGNGLVG
                     IVYDVSSHTAKVRTIINDTSMVSAMFLKTNDACIVNGSLDTMEDGYLEVVYISKDAKV
                     KNGDELVTSYVSSKFNEGITIGKVSDLKLDSTKLTKTAKVTPVVDFKHLKEVLVITDL
                     KKTKNLDEETKE"
     gene            complement(11768..12727)
                     /locus_tag="PLC71_00070"
     CDS             complement(11768..12727)
                     /locus_tag="PLC71_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRM01073.1"
                     /translation="MKTAQLGIELGTRHLRICTRDKEEFLKIKNMIAFDEDAKLKAFG
                     NEAFAMYEKQPYSIHVVKPMEHGVISDYTNMRYLLGTVLDHYFKRIRKTGLMIAVPVD
                     ITNVEKRAFEDLAWESASRVKDVTLVPKPILAAIGSGIDVNAPCGNMIIDIGASTTEI
                     SVISLGGIVTSRLLPYGGDQIDEWIRDYVKKNYKLAIGTRSARHLKLAYAKIEDKEAA
                     MIKGRNLVSGLPKQEKIPIQIVEEKAHSQVRDICVQVKAVLESITPELASDIMNQGIY
                     VSGGGAAFPKTAEWIKEEFKIPVHIADRPEDHVILGMQSYLKR"
     gene            complement(12738..13436)
                     /gene="radC"
                     /locus_tag="PLC71_00075"
     CDS             complement(12738..13436)
                     /gene="radC"
                     /locus_tag="PLC71_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRM01074.1"
                     /translation="MEEKHQTLKELPVSERPYEKCEKNGTAMLSDAELLAVILRSGTK
                     DQTAIDLATKVLSIDPFYEGILGICHTSREELQKIPGIGKVKAMQILCIAELSKRLAS
                     AKVEDKISFHSPASIADYYMERMRHLSREEMILIFFNGKNKVIKELTVSVGTVNQTVA
                     SPRELFLEALRCEAVSVLMIHNHPSGDPTPSRQDILTTKRMKEAGEFLGIPLCDHIII
                     GDQTYLSFREEHMM"
     gene            complement(13561..14859)
                     /locus_tag="PLC71_00080"
     CDS             complement(13561..14859)
                     /locus_tag="PLC71_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HRM01075.1"
                     /translation="MMDQLKNYYKEMGVDEKVFDYAQEVEKGLKDRFETLDAIAQLNQ
                     IKVLNAMQKHQIAEMHLGGTTGYGYNDSGREAVEALYADVFGTEDALVRPQITCGTHA
                     LGLALSANLRPGDEILAISGKPYDTLEEVIGIRESKGSLKEYGITYAQVDLLEDGSFD
                     LEGIKNAINDKTKLIHIQRSKGYATRPTLSVDVIGEAIAFVKEINKDLIVMVDNCYGE
                     FVEEKEPSEVGADMIVGSLIKNPGGGLAPIGGYICGRSDLIEQCAYRLTTPGLGKEVG
                     ASLGVNRQFIQGFFMAPTVVNAAVKGAIFAANLYEKLGFKVIPDGKEDRHDIIQAVEF
                     HDPEKLIAFCEGIQKASPIDSHVTPIPWDMPGYDSQVIMAAGTFVSGASIELSADGPM
                     KEPYAVYFQGGLTYPHAKYGIIMSLQRMAEKGLVEIPEIS"
     gene            complement(14831..15793)
                     /gene="miaA"
                     /locus_tag="PLC71_00085"
     CDS             complement(14831..15793)
                     /gene="miaA"
                     /locus_tag="PLC71_00085"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRM01076.1"
                     /translation="MKPMVILTGPTAVGKTELSIRLAKILDAEIISADSMQVYKKMDI
                     GTAKIMPEEMEGIKHYLVDELDPKEEFHVARFQQMAKDALKEIYAKGKIPLIVGGTGF
                     YIQSLLYDIDFTKEDGDQEYRKQLEEKAKREGNDSLYEMLKQVDPKSTEKIHKNNVKR
                     VIRALEFYKLNGYPISEHNEKEAAKTSPYNFAYFVLNHKRDILYDRINRRVDIMAEQG
                     LIDEVKRLKEEGYEKTMVSMQGIGYRQVFEYLEGNLSLEDTIEQIKKDTRHFAKRQLT
                     WFGREKEVCMVDKSQFGSEDEILTYMLTILKEKGIYDGSVKELL"
     gene            complement(15798..16175)
                     /gene="cdd"
                     /locus_tag="PLC71_00090"
     CDS             complement(15798..16175)
                     /gene="cdd"
                     /locus_tag="PLC71_00090"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRM01077.1"
                     /translation="MTDQELLDLADKAKEKAYAPYSRFQVGAAILTKEGKVFTGCNVE
                     NSSYGAAICAERTAAVKAVSEGYHDFEMIAIASNGSDKETVYPCGICRQFLHEFNPDL
                     KVICQSPDGFETYRLSELMPKGF"
     assembly_gap    16337..16346
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16347..18200)
                     /gene="mutL"
                     /locus_tag="PLC71_00095"
     CDS             complement(<16347..18200)
                     /gene="mutL"
                     /locus_tag="PLC71_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRM01078.1"
                     /translation="MAKIQLLDQKTIDNIAAGEVIERPASVVKELVENAVDAKANAVT
                     VEIKDGGMTLIRVTDNGIGIPKDQVKTAFLRHATSKIRSVEDLLSVSSLGFRGEALSS
                     ISAVAQVELVTKTAESFSGVSYKIYGGEEEAFEDIGAPDGTTFLVKNLFYNTPARRKF
                     LKSATTEAGYVEQMMVHIALSHPEISFKFIHNNKNKIYTSGNGKVKDIIYHIYGRDVA
                     GALIPLEAQNEDAKPYVSRGNRNYESYFINGRYIKSSIIYKAIEEGYRTFTMKHRYPF
                     VCLDFKIDQELLDVNVHPTKMEIRFRNGREIYELVVDAVRAALLQRDLVQDVLRETPK
                     KKELPKTKEVKKPEPFEVNRRKEEVQKVDQQGQGVQQQVEKQRQAVQQQIEKPSHPVK
                     KTLTASESSKNTKKPTYAGLNYNTQKKEFPQYKTDELSSNQMTLREDPVFSVQARPDR
                     KILGQLFKTYWLIEYEDQLFIMDQHAAHEKVNYERLMKNFKEKEIYSQRLEPPMVVTV
                     SMTEAEALTRYKDAFAGLGFTIESFGGNEYCIREVPANLYGIGERDLFMELLDAVSQE
                     NGTLDTEVIASKIATMACKMSIKGNQRVSLMEVEHLLDELMKLENPYQCPHG"
     gene            complement(18205..20832)
                     /gene="mutS"
                     /locus_tag="PLC71_00100"
     CDS             complement(18205..20832)
                     /gene="mutS"
                     /locus_tag="PLC71_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRM01079.1"
                     /translation="MAKKGKLTPMMQQYMQIKEENKDCILFYRLGDFYEMFFDDALTA
                     SKELEITLTGKNCGLEERAPMCGVPYHAVDSYLNKLVEKGYKVGICEQVEDPSQAKGI
                     VKREIVRIVTPGTNISQQSLDDEKNNYLMCIFANDGSYGISFVDVTTGDFRTTSMDSL
                     AKVRDEIFKFEPAEIVCNDAFLISGMDFDYLKDKMSIVISSIEPYHFDEEQAEERIKR
                     QFKVGNLEGLGLLDHPMGVIATGALLGYLHETQKSSLDHLMHIEAYETSEFMIIDSSS
                     RRNLELCETLRDKQKKGSLLWVLDKTKTAMGARMLRNMVEQPLVDKKKIEERYDAITT
                     LTDQTIVREELREYLNPIYDLERLMTKVSYKTANPRDMIAFKTSLELLPAIKTVLEEC
                     KDPLLSGLREDLDPLEDIHNLLEDSIIEEPPLAIKEGGIIKEGFKEDIDKLKRAKTEG
                     KQWLMELEEREREKTGIKNLKIKFNKVFGYYLDVTNSYKDLVPDHYIRKQTLANSERY
                     TTEELNQLADTILGSEDRLYALEYETYVMIRETLAGEMERISRTANVIAQIDALASLA
                     YVAERNHFVRPKLNVRGTIDIKDGRHPVVEQVIPNDMFISNDTYLDNKKNRVAIITGP
                     NMAGKSTYMRQVALIVLMAQIGSFVPAAKANIGIVDRIFTRVGASDDLASGQSTFMVE
                     MSEVANILRNATKNSLLILDEIGRGTSTFDGLSIAWAVVEYISNSKLLGAKTLFATHY
                     HELTELEGKIDSVHNYCIAVKEQGDDIVFLRKIVKGGADKSYGVQVAKLAGVPDAVIN
                     RAKEIARELEEHDLTSGAKDIMPYGEAQQLSFFRESDEPTMEHPFMAELREKDLSDMT
                     PLEALNYLYVLQEKLKNEE"
     gene            complement(20825..22237)
                     /gene="miaB"
                     /locus_tag="PLC71_00105"
     CDS             complement(20825..22237)
                     /gene="miaB"
                     /locus_tag="PLC71_00105"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRM01080.1"
                     /translation="MEQLKNESSRQYEYMEEMKKWVQKKSGELGRKLTCHVTTFGCQM
                     NEKDSEKLLGILETIGYEEVETEEADFLIFNTCTVRENANTKLYGHLGQVKKMKERNP
                     QMMIGLCGCMMQEEHVIEKIRSSYKFVDIIFGTHNIFKLAELLKARVDSKGMIVDIWK
                     DTDQIVEDLPSDRKFSFKCGVNIMYGCNNFCSYCIVPYVRGRERSRKPEDIIKEVKQV
                     VSEGVKEVMLLGQNVNSYGKTLEEPMSFAELLREVEKVEGLERIRFMTPHPKDLSDDL
                     IEVMATSKKVCKHMHLPMQSGSSRLLKLMNRHYTKEQYVALAKKIQERIPGVSFTTDI
                     IVGFPGETEEDFKETLDVVREVGFDSAYTYVYSKRSGTPAASMEDQVDKDVIKDRFDR
                     LLALLKETSAKNCKKKVGDIERVLIEEENTHEEGMLTGRLENNLLVHFKGCKEQIGTM
                     ADVKLVEEKGFYYMGEQIHG"
     gene            22445..24127
                     /locus_tag="PLC71_00110"
     CDS             22445..24127
                     /locus_tag="PLC71_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRM01081.1"
                     /translation="MNYTIILALMGGLGLFLYGMKLMSDGLEKAAGAKLRRILEIFTT
                     NRLTGMLVGIFFTAVIQSSSAATVMVVSFVNAGLMNLSQAAGVILGANIGTTVTSQLV
                     SFNLSEIAPVFLMAGVIMVLFMNNPTVQKVGEVILGFGVLFMGLTSMSSAMSVLRDSP
                     LITSYLQTLDNHFLGVLFGFIMTAILQSSSVTVSIVLLMASQGLLHLPICFFIILGCN
                     MGSCVSALLASLSGNKGAKRAAMIHFLFNVFGSIIIFTGLSFALTPITNFFLSISGGN
                     LGRSVANAHTTFKIIEVLFMFPCTPLVVALTEKIIRGKDEKVHHNELQYITEISLKSP
                     ATMIPQAKNELRRMANMAQENLDHAIHGFLNQSDEFVDKIYHREEDINNVSHAITDYL
                     VKSNQLSLPLADQKILGSMFYVVNDIERIGDHAENFADFTKTEIKHNTGLTGDAKEEI
                     KKMYTAVSKLLKLSLECFMEQDGVNKPEEKLAEIAILEASIDKMERRYQKHHIKRLAK
                     GECEPRAGLLFSDMLSELERIADHSVNIAYSMSDEDEDEILAAENEALTAKN"
     gene            complement(24222..26144)
                     /locus_tag="PLC71_00115"
     CDS             complement(24222..26144)
                     /locus_tag="PLC71_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRM01082.1"
                     /translation="MIHNASVEKYRTPVGAVTCGSEMMIRLYDVGQEVNKAEIIMYSE
                     SFHREYEMKRKKDCMEEHIKMTSEAGVVWYYFRIWEHGKCYFYGARHGKTQGEGMIVD
                     DNPDSFQITVYEENFDTPRWMSKGIMYQIFPDRFCQGNKENMKRGKVYHESMGRTVYL
                     HDDWEEQPVFGPLPGKEFYDPCDYFGGDIEGIISKLDDLKRLGISCIYLNPIGEAASN
                     HRYNTSDYKKVDPFLGNNEDFKRLCQLAKERRIHVILDGVYSHTGDDSVYFNKKGNYE
                     EPGAYQGEESKYYDWYDFNEDGTYKSWWGFETLPEVNELNEKFVDYIITGEDSVIKHW
                     LKLGASGFRLDVADELPDEFIFKLRSELKKKFKNYALIGEVWEDVTTKESYGTKRKYA
                     LGKGLDSAMNYPLKVNVTDYLLGNQSAKDMKTFLISQQCNYPKPLYYALMNLLSSHDI
                     PRIRTTLATGMNENLPSREQQAEYVITSKMDQKGKALTRLAMAVQFFVPGMPCIYYGD
                     EYGMHGLMDPFNRGAFFENDKETYQEVLRLTSLRNREKVLQTGYALYMAPTENVLAIL
                     RFISEKKDVFGEASENKAILLLVNPTMKKEEINLDLNKVKEGVNENALQALLKNLSNA
                     VIKTNVAPIDYKVIEL"
     gene            complement(26172..>26707)
                     /locus_tag="PLC71_00120"
     CDS             complement(26172..>26707)
                     /locus_tag="PLC71_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRM01083.1"
                     /translation="ISEQISTAGMEASGTGNMKFMMNGALTIGTMDGANIEIAERVGQ
                     ENIFIFGDSAEGNYNKKMYHTYNPGIIFENHPGIRDVCNCLIEGNLLRYGNRKYSEIY
                     QNLLFGEYGEADPYYILADFPSYIETYEKVYHLYVDHKDEWIKKAIVNTAKSGYFSSD
                     RTIEQYNEKIWNLKPVK"
     assembly_gap    26708..26717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26718..28616)
                     /gene="glgP"
                     /locus_tag="PLC71_00125"
     CDS             complement(<26718..28616)
                     /gene="glgP"
                     /locus_tag="PLC71_00125"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HRM01084.1"
                     /translation="MDYMDSNKIGQRIENKLIHALGRTISEATNDEIYKAAAECIRDD
                     IMISWADSRKRVQEQGVKKLYYMSAEFLMGRAFSNNLISQGLYEAYREAFWEMGLDFD
                     KITDEENDAGLGNGGLGRLAACFLDSLSTMELPVLGCGIRYEYGMFRQKIVDGTQIEM
                     EDDWLRDGNVWEIERPELSVEVHFNGTIQENWTENGLKIEHKDYNTVIAVPYDVPIIG
                     YKTKTPATLRLWSARSKRRFDFHSFNEGIYDKAMADQTFAEAISKVLYPSDDHMQGKM
                     LRLKQFYFLASATMQSMIKRHKAVFDDLNSLPEHVVIQINETHPALAMPELMRILMDE
                     EDFGWDEAYGMVKKIFHYTNHTIMSEAMECWDENMFRLLLPRIYQIICAINEKYCQKL
                     SVYYSKEEEKIAQMAVIGNNEIRMANLCVALCRRINGVSNLHADILKTRIFKGSYQIF
                     PQKYLAITNGITHRRWLALANQGLYHLVREYVKGDILKDYRLFEQILPYKDDKEFCKK
                     YEKVKRNNKIRFAKYIKEKQGIEVNPESIFDVHCKRLHEYKRQLLKCLHIIYIYQKIK
                     KDPACITTPITFIFAAKAAPGYARAKEIIRLIHSIQEMVNKDPDMDGKIQVVFVENYC
                     VSVAEMLIP"
     gene            complement(28729..29415)
                     /locus_tag="PLC71_00130"
     CDS             complement(28729..29415)
                     /locus_tag="PLC71_00130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM01085.1"
                     /translation="MNQEEFNPETDPLIPYHFNKDDLSGKKKDKQELIKALGLSITPG
                     VPIIGMVTRLTEQKGLDLVKYMLDEIMCTENVAFVILGTGDYEYEEFFRYMEEKYKGR
                     VCSYIAYDNAVAHQIYAGSDLFLMPSKFEPCGISQMIALRYGTLPIVRETGGLRDTVQ
                     SYNEYEDTGNGFSFANYNAHEMLGAIRYALQTLQSPKRKHGLIQRAMESDNSFAKSAK
                     IYLDMYNSVL"
     gene            complement(29396..30121)
                     /locus_tag="PLC71_00135"
     CDS             complement(29396..30121)
                     /locus_tag="PLC71_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRM01086.1"
                     /translation="MSNNNSLKVLLAGAECAPFVKLGGLADVMGTLPKELNQIGADAR
                     VIMPFHSQMKGKYGAQTRHLADFNINFSGGETYVGVEELVYNEVTYYLIDNEGFFGDA
                     VYRGGLAEGEQYAFFCRAVIEAASRIGFIPDVIHCNDWQTGLIPILLRTQYGHWDIGH
                     AKTIFTIHNMMYQGEFGFDQFEHWLGIDPKYDTPEFMHNYECASFMKAALVFADRLST
                     VSPTKCESRRYMGNLKWNESGRI"
     gene            complement(30114..31247)
                     /gene="glgD"
                     /locus_tag="PLC71_00140"
     CDS             complement(30114..31247)
                     /gene="glgD"
                     /locus_tag="PLC71_00140"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HRM01087.1"
                     /translation="MDKQMLGIINAGEENRKLKDLVRSRSLSALPVGGRYRTVDFMLS
                     NMVNAGIKNVGLLTRRNYDSLLSHVNSGKYWGLNKKRGGLYIFPPYINDASPSGEYAS
                     VMDGLRGTLGFLKKAKEKYVLLGRSYVICNADFQDMLDKHIKSGADITLMYFDNSDDS
                     TNCDYDGVYLKTDEDDKVLDMCYSEGKHRSNKKSMDAYIMKTTLLIDFIETAIAHNKK
                     HFFFDVIVPNFDRLVIQGYEYKGYVGCITSLEAYYNVNMDMLKPDVYKELFLGNRRIY
                     TKSADGAPAKYGPNAKVSNSLISSGCEIDGEVENSVIFRGSQIAEGAVIKNSVVMAEG
                     FIAPQAEINHTILDRHVKVYSNAHLSGSSNHPVFIPKGASVNE"
     gene            complement(31261..>31966)
                     /locus_tag="PLC71_00145"
     CDS             complement(31261..>31966)
                     /locus_tag="PLC71_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRM01088.1"
                     /translation="EAKRFGIMNTDEDERIYEFEEKPAEPKSTKASMGIYIFKWKLIR
                     QYLIDDGNDPESEHDFGKNIIPRMLEDGRKMYAYSFDGYWRDVGTIDSLWEANMELLD
                     ENPQIDLNDDKMRIYTRNFSLPPHYIAPGASVKNSILADGCVIEGEVENCVIHSGVKI
                     GKGSVVKNSVIMKDTVVRTDCQIDKALIDEEVVIEQGCKVGEGNNVTVIGYHCVALEN
                     ATVEDGAMIYPDTILH"
     assembly_gap    31967..32002
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<32003..32472)
                     /locus_tag="PLC71_00150"
     CDS             complement(<32003..32472)
                     /locus_tag="PLC71_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRM01089.1"
                     /translation="MINKKEIVAMLLAGGQGSRLFALTQKKAKPAVSYGGKYKIIDFP
                     LSNCTNSGIDTVGVLTQYEPLELNAYIGTGGFWDLDSLNGGAYVLPPYMKGETGNWYR
                     GTADAIYQNINFIDNFDPDHIIVLSGDQICTMDYDKMLQYHKEKGADCTISVLNV"
     gene            complement(32489..33343)
                     /locus_tag="PLC71_00155"
     CDS             complement(32489..33343)
                     /locus_tag="PLC71_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HRM01090.1"
                     /translation="MAHPGKKLLFMGGEFAQFVEWRDKEQLDWFLIDQYEMHDSFHKY
                     VAKLNEIYKSEPALYELDQDPAGFEWCLQRDADHSVVAFIRKNKKGRGRKQQQILCVC
                     NFTPMEWDKYKVPLPKKSKLTKILDSSELDFGGDGDVSESKVSTKRVKVPSEGKKAVY
                     QQAAEISLKPLSVVYYKIEEVETKPRKSVAKKAEPAKKATAKKAEPAKKAAQEKKTEA
                     VKKTVEKKPEVTKKVTKEEAPETKKDTPKKEDIKVTKQTTEKEEEQPKKRTSKTRRTK
                     KNNDQGVK"
     gene            complement(33286..34719)
                     /gene="glgB"
                     /locus_tag="PLC71_00160"
     CDS             complement(33286..34719)
                     /gene="glgB"
                     /locus_tag="PLC71_00160"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRM01091.1"
                     /translation="MNETINREDLYLFQTGKARQAYLTFGCHYQKKTKTHRFTVWAPN
                     AKSVSVVGDFNFWNPLAHPMKGDKDGIYTIEIEGLKKGDLYKYHVEGYDGICRYKSDP
                     FAFYAECRPDTASKVWDFDDFKWSDKRFINQRKKRQALDQPMSIYELHLGTWRMPQEE
                     EREFYNYREIADMLIPYLGEMGYTHVELMPITEYPYDLSWGYQVTGYYGVTSRYGTPE
                     DFNYMINELHKAGISVILDWVPAHFPRDEHGLAMFDGTHIYDHEDPRKGSQPDWGTLL
                     FNYGKPEVQSFLISSAMFFADVYHIDGIRIDAVSAMLYLDFGKQEGEYVPNEDGTNIN
                     YEAVDFLRNLNEALRTTHEGFLTIAEESTAYPKVTSDVDDPEGLGFVYKWNMGYMHDT
                     LYYMELDPLYRKDNHGAIIFSMDYAYSENYILPYSHDEVVHGKGSMINKMYGAYDEKF
                     ASLRTMDLPWHIQERNYYLWVENSPNL"
     gene            complement(35144..36361)
                     /gene="ilvA"
                     /locus_tag="PLC71_00165"
     CDS             complement(35144..36361)
                     /gene="ilvA"
                     /locus_tag="PLC71_00165"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRM01092.1"
                     /translation="MLELKDFEAAYNKVQEVVLPTKLIKSDYFSDQTGNDVYLKPENM
                     QLTGAYKIRGAYYKISTLSEEEKAKGLITASAGNHAQGVALAAARQGVKATIVMPTTT
                     PLLKVNRTKDLGAEVILHGDVYDEACAHALDLAAEHGYTFVHPFDDPTVATGQGTIAM
                     DIFKELPTVDYILVPIGGGGLATGVSTLAKLLNPKIKVIGVEPAGAACMKASLEAGKV
                     VSCDHVSTIADGTAVQTPGKIIFPYIQENLDDIITVEDEELIPCFLDLLENHKMLAEN
                     SGLLTIAALKHLDVKNKKVVSIISGGNMDVITIASLVQHGLIMRDRIFTVSVFLPDKP
                     GELNKVSDVIADAQGNIIKLEHNQFVSINRNSAVELTITMEAFGTEHKNKIIKSLKKA
                     GYDPKMRNSTSVY"
     gene            complement(36363..37298)
                     /gene="ftsY"
                     /locus_tag="PLC71_00170"
     CDS             complement(36363..37298)
                     /gene="ftsY"
                     /locus_tag="PLC71_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRM01093.1"
                     /translation="MSEKKGFFKRLAQGLSKTRDNIASSFDSIFSGFSGIDEDFYEEL
                     EEILIMSDMGIDTTMNIIEDLKKKVKENKIKEPEECRQLLIDSIKDQMRLDDSAYDFI
                     DKKSVVLVIGVNGVGKTTSVGKLAASLKGQGKKVLLAAADTFRAGAIEQLKEWSNRAG
                     VDIISQAEGADPAAVVYDAVAAAKARDVDVLLCDTAGRLHNKKNLMNELSKINRIIQK
                     EYSEAYLETLIVLDGTTGQNAMAQAKQFKEATDISGIILTKLDGTAKGGIAIAIQSEL
                     GIPVKYIGIGEQVDDLQRFDPDMFVNALFEKGEEN"
     gene            complement(37312..>38652)
                     /locus_tag="PLC71_00175"
     CDS             complement(37312..>38652)
                     /locus_tag="PLC71_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM01094.1"
                     /translation="RKEELDNRHKQLSIQRQQLEEELRNAEDDADRLQDEQVDLESAN
                     EQDEERIKEISEILEHLRKHAEQHAMEVSKVHMQLSKESQKLEFQENNQERVRSEIEH
                     LSEEVQENQKDTGSINENVKALKQQIGHLKESCEAMERKIIEDQNRHQKMQEEVKEKE
                     VLYKDILKEREEMLERISGYDKEMYRLESQRENRKGKKKELLDYMWENYEITYHQAKQ
                     RIDLDETQSLTKVKETISELKNKIKNLGPVNINAVEDYKEVSERYEFMQKQHEDIVTA
                     EAHLTGLMEELEDAMRRQFNAKFKDIKRVFQEVFVELFGGGEARLELTDDDVLESGIR
                     IIAQPPGKKLQNMMQLSGGEKALTAISLLFAIQNLKPSPFCLLDEIEAALDDSNVTRF
                     AKYLHKLTKDTQFIVITHRRGTMMAADVLYGITMQEKGISTQVSVNLIENDLDE"
     assembly_gap    38653..38662
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<38663..40865)
                     /gene="smc"
                     /locus_tag="PLC71_00180"
     CDS             complement(<38663..40865)
                     /gene="smc"
                     /locus_tag="PLC71_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRM01095.1"
                     /translation="MYLKSIEVNGFKSFAHKMIFKFEHGITGIVGPNGSGKSNVADAV
                     RWVLGEQRAKQLRGSRMEDVIFSGTELRKPMGFAYVAITLDNSDHSLPIQFEEVTVAR
                     RVYRSGESEYLINGSACRRKDIVELFFDTGIGKEGYSIIGQGQIEQILSGKPEERREL
                     FDEAAGIVKYKKNKLETQKSLEIERENLVRVTDILTELERQVGPLKKQSERAREYLLL
                     RDQLKDEDVKLFLLENQNLEKELKELDEKIEIAQREINEANQTLAKAKEKYGKQEEFL
                     DHLKQEIETITAHISEMKLTKQKKEGKIQVLNEQINTEHMRESMYLESKDKLYKDLNE
                     KKAQLKRFEAETEEIRKEYESVMEQKIQKESEVALYQKQIQDLEREQEEIEIRVNAVR
                     EGRMRVAGAIERSQTVKEQLNVRIIGLDHQIEEIKRQINDQEKEKDSSSQKIEEINEK
                     KKRSEIECHNAKRIEETLIAERRQIQEQMDQKREKYHRVRSNYETMRNMAERYDGYGF
                     GIKKVMEQQAVTSGIIGAVADIIKVEEKYETAIETALGGSIQNIVTDTQKTAKKMIEY
                     LKKNRYGRVTFLPLDAVKGKEFARKEILLEPGVIGAANQLVIYDEVYKDLFASLLGQI
                     LVVKDMDAGIKIANKYHHSVRIVTLDGDSLNRGGAMSGGAFKNKSNLLGRSREVEELK
                     KKLDRWTKAITEDEEKYHENEKKLKEYQQNMIDLEKEMQEISLELNTQQMSQ"
     gene            complement(40884..41570)
                     /gene="rnc"
                     /locus_tag="PLC71_00185"
     CDS             complement(40884..41570)
                     /gene="rnc"
                     /locus_tag="PLC71_00185"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRM01096.1"
                     /translation="MDAKSVEEFQEQIGIKFHEPSRLWNAFIHSSYANERRMSKDKNN
                     ERLEFLGDAVLELVTSNYLYRTYQKESEGKLSKIRASLVCEPTLAGCARDINLGKYVL
                     LSKGEDMTGGRSRDSILSDAFEAVIGAIYLDQGIETATKFIETYLFKDVENKVLFFDA
                     KTNLQEIVQGEGKGRLKYVLVGEEGPDHAKIFTVEARLGENVIGTGSGHSKKSAEQHA
                     AFEAIKRHKK"
     gene            complement(41578..42585)
                     /gene="plsX"
                     /locus_tag="PLC71_00190"
     CDS             complement(41578..42585)
                     /gene="plsX"
                     /locus_tag="PLC71_00190"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRM01097.1"
                     /translation="MEKLVKVAVDAMGGDHAPGEVVKGAVDAVNEKNNLKVFLVGKAP
                     RIHEELSKYQYKNEQIEVVNAEEEISCDESPVEAIRKKKDSSMVVALKMVKDKTADAF
                     VSAGSSGAILVGGQFVVGRIKGIKRAPLGAVMPTAKGPMLLVDAGANMDAKAEYLLQF
                     AQMGSIYMEDVLGIKNPRVGLVNVGVEEAKGNQLVKETGPLLKECENINYIGSIEARE
                     IPYGAADVVVCDAFAGNIVLKLEEGLAAVLVSQIKEGMMSTLKSKIGALLAKPALKKT
                     LKKFDASEYGGAPLLGLNGLVVKAHGSSSAKEMKNAIIQCIAFAEEDITQKIKDQIVK
                     G"
     gene            complement(42680..>42779)
                     /gene="rpmF"
                     /locus_tag="PLC71_00195"
     CDS             complement(42680..>42779)
                     /gene="rpmF"
                     /locus_tag="PLC71_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRM01098.1"
                     /translation="KCSKCGALMMPHRVCKACGSYNKKEIVSAHAE"
BASE COUNT        11017 a         9907 c         6653 g        15116 t
ORIGIN      
        1 cgtgaataat cacgtgcgcc gaaaataccg gttcctacac gtaccatggt tgccccttct
       61 tcaatcgcta cttcataatc attggtcatt cccatggaaa gtatactcat atctacatta
      121 tcgaagtttt tgttgttgat gtcaacagat aattgcttca tcgcacggaa atacttacga
      181 ttgtcttctg gatcgtcaac aaatggtgca atctgcatta accctttgat ctttacatgt
      241 tctaaggcag aaatctcttt caccacatca aaaacatctt ctggattcaa tccaaatttg
      301 gtatcttcgt gtgcaaaatt cacctggatc agaattggaa cttctttgac atcatgcttt
      361 gcagcttctt tgtcgatcgc ctgtgcaagt tttatggaat ctacagaatg gatcaatgct
      421 actttatcca cgatatattt aattttattg gtctgaagat gtccgatcag atgccatctt
      481 accggccaac tgatatttgc atatttatct ctgatctcct gtaccttatt ctctccaaaa
      541 tcaagtactt tttcttttct taattcttct accatttcta atggtttggt cttgctgact
      601 gcgatcactg tcacttctcg tggatcacgt cctacacgtt tacaagcttc ttctacatgt
      661 ttctttactt cattatagcg ttcgatcaac atatccaatt ccttctttct gtaattctta
      721 taaatttata tgccttaata tatgatctgg ttttccttgn nnnnnnnnnc catcaagaac
      781 aatgaaatca taccgactaa ctccctgatt ggtatcattt gcaatgatcg aataatcatc
      841 tttctcgatc agaatatcga ttggacggaa atccgcatat ccctgatttg tacaataaac
      901 tccttttaat gtcttcattt tctcaatcaa aaatgtatct ttggaatcca ttgcctgaag
      961 cactacgttc tctccaactt cttctggatc aagataacag taatccttcg ttcttccata
     1021 gatcgtaaag ctcttttgtt ccaccgtcac atcgcctttg ttgcttgtat cacgtacggt
     1081 aaactgttct ttcgcactgt tatctccttt tgtaaggtat tttactggaa cctggtagaa
     1141 tttcttcttt acaatggatg tgtttggaat ctttaatccc ttgtaggaat caagcgtcac
     1201 atcgatttct aagtagcgct cattgatata acgnnnnnnn nnnatcatta aacttaagat
     1261 atccaaaata cgaacttccc tttttggtga cttccaccgt tgctgtggct gttgtctgat
     1321 cttgcaggaa acgaaccgac acttcacttt tatccttcaa catccgatac tgatctgctt
     1381 ctagtggaat tacgatctgc catttttcgc ttttgttgat cctagctaca actttgcctt
     1441 tctttacttt atcggacgta ttatacctct gaacacgata ctgactttgg tcaaaatcct
     1501 tgcttgtgat atcttttgcg ctcttaccgt caaagtcatc agaataaaat tgtgttaccc
     1561 cactgcttgt acttttctgt gtctcaaaac ttgtatcccc aagcttttgt ttgactgctt
     1621 ctataactgt tttcatagaa tcttcactta agttgaggat tgcattcttg agatcatatt
     1681 tcagatcata aaccgtgctg aacttatgat cactatatac agactgataa tctgtgatct
     1741 tatgctgaat ctcttcatca tcgatcactt ttttattttt ctcaagctgc tgcacctgac
     1801 ttacaaatgc atcctgaacc ttctgatctt tatcaaccgt aaagaccgtt cctgatttct
     1861 tgatccgttt tccttcttct acataatagg taatataacc gctttgtgaa gcctttagaa
     1921 gttcctcatc acggatcgca attcctgtaa acgataaggt attggtgatc ttatcttccg
     1981 tcaccttgta gacagtcagc tttttcttgc ctagaaacag gatgacatta atcaggatgt
     2041 agaccaatat gactacaaaa atgatcattc caatattcca atgtattttt gttgttcgtc
     2101 ttcgttttgc tgccatctct taaatctccc atcgcctgag cgctgctcca tttaaatagt
     2161 caaatttaca tccttgcata tagtcatttg tttccatgat ctcgtcaaat cgatctttta
     2221 atttccacca gctgatgccc cagttagaaa atgatgcatc ttccttgggc actctgctga
     2281 atagacgctc gatcaccata tcttttcgta cataacagat aaagtttacc agtctgtcca
     2341 gatattcttt tggatcacaa agagttattt ttccttcttt atactcctca tacatttttg
     2401 atcctttggg aatataaaga gaatgtaatt ttacgatctg cactggaagt gcagaaagaa
     2461 tctttgctgt ctcgatcaca tccgtcatgt catctcctgg cagattcaaa ataatatgag
     2521 tgcagatctc aaatccatat ggtgctgtca ttaacacagc atctagatat tctgccagtg
     2581 tatgtcctcg attgatcttg tttaaagtct tataattgac tgtctgaagt ccatattcca
     2641 tagagatctt cacctgatat tcttcagaaa tcttctttaa acagtctaaa tattccttcg
     2701 taatacagtc aggtcttgtg gataccgaaa ttcctacaat atcttccgtc tgtgctgctt
     2761 ctaccagata cttttcaaac ttgtcaaccg gcaggaatgt gttcgtataa ttctgaaaat
     2821 atgcgataaa tttctttgct ttatagcgtt ttgtgatctt tccttttgtc gcattcagct
     2881 gttcacttac agatacctca ctgttcatag cttcaaagcc ggttcccaca cctccacaga
     2941 aagtgcaccc atctccatcc atacggtttg gacaagtcac cggaagatta actggtaatt
     3001 tatatacttt ttctccatat aattccttta aatgtgtgct gtaagctttg tatcgttcca
     3061 tcttagtttt cctttgataa tatatctgtc attgtatggt aaagagctga ttttgtaacg
     3121 ccttttaaca ccacggaaat gaattcctca ccgtctttat tctttgtaag aagaatcagg
     3181 caagaacccg cctgtgttgt tgtaccagtc ttgcctccga tcatatatac ctggaagtgt
     3241 gtatgttccg gcaatatact ggtttgtgga agccatatca taaatctttt gttctccctt
     3301 ggaaccaaga tattcaatcc tgctgcttga tcttcctgca atattaaaga attcattatg
     3361 tttagctact tctttaaaga tcagataaag atcatacgct gtagaataat gatcacttaa
     3421 atgaagtcca ttcggatttg caaaatgtgt attggttgct ccaagttctt tggctctctt
     3481 attcatcatt tttacaaaat ttgcttcact tccagccact gcctctccaa gtgcaacggc
     3541 tgcatcattg gctgacataa tgatcaatgc atttaacaat gcttccactg tgatcttatc
     3601 ccctttcttc aagtggatcg ccacagctgc tccatcaggg aacgtaatgt catgtttgat
     3661 cgttacaacc tgacttaaat ttaaattctc caaagtcagc aatgctgtca tgatctttgt
     3721 tgtacttgcc gggtaagcct ggtcaaaaac cttctgacta acgatcactt tattctttgt
     3781 attattaacc aacagaactt ccttggcttc cggatagtcg ttattatccg ccttttcttt
     3841 ggatacaacc gctaccttag aacctctgga ttcccaacgg tcactcaatg catcttcttt
     3901 taatgtcgtt tcattcttct gataatgttc atcatatgat gtaaatccac ctgtcttcgc
     3961 cgatgtctgg cagccagata atgcgatcat catgacggac atacaaatac aaattaattt
     4021 tttcatttta tgctttctgt aaaaaaagac cgtcgtgtga cagtctattt atacatattc
     4081 ctccaatcta aatctcctcg gtccagtgcc aaaatcaata cttctgccgt tggcagattc
     4141 gtagcacacg gaatattata ctgatcacag agatttaaaa cattataaaa atctggtgtt
     4201 ctcttcttat ttgccgggtc ctgtaaaaag atcaagaggt ctatttcatt attggagatc
     4261 ttagatccca tttgctgcat accgccaaaa tggccggcaa gaagtttgtg gatctttaag
     4321 tttgcaacat cttcaatcat ctgtccagtc gtacttgtag catataattt atgttttttt
     4381 aaaatatcct gatacgcgat acataaattc tgcatcaggt tctttttgcc atcatgggca
     4441 actaatccta cattcataga tagaacctcc tagtgtcttt ttctttgtaa atatatatta
     4501 agtaccattc ctatcgcgat tgacgccgac attaaggaac ttaatccata actgataaat
     4561 ggcaggggct gtccggtgtt cggcatggtt cctgtagcaa ctgcaatgtt gataaaagac
     4621 tgaaatccca tataacacgc aactcctaca gccacataag ttccgatcag attccctgat
     4681 ttcttcgcca cattcatgca ctgatataca aggcatgcaa aaacaatgat caataccaca
     4741 catccaacaa acccaaattc ttctccgatc acagagaaaa taaagtctgt ctgattttct
     4801 gataccaaat ttacatcact tgcagataca tttgagatcg tattacttcc aaatcccttg
     4861 ccaaatagtc cgccagcacc gatcgccatg atcgaattgc ttgtctgtaa agcagtggaa
     4921 tctgcgtaat tctctggatg taaaaaggac atgatccttg ctacctgatg tggctctaac
     4981 aacacctgtc ctggtgtctg gacataccac aaaaagattg ctaaaagtgg tacgcctacg
     5041 atcaatacct gaacgatcaa tctggaggat aatcctgcca taaagatcat tccaagaata
     5101 ataaaacaaa tatcgagtgt agaagaaaga cttggctgtt tcaagatcaa aagcagcggg
     5161 attccacaga ttacagcaag ttttcctaat accttcggtt cattcagatc atcctgatgc
     5221 ttcgcaagaa atgctgcaac aacaatgatc atgatcactt tactaagttc tgatggctgg
     5281 aaagttccca atggacctaa tgaaaaccat cgtttggcac cattgacatc ttttccaaat
     5341 aatttaacgc cgatcaacat gatcacattc aaaatataca atatttctgc atttcttgta
     5401 aagacctgat aatcgatcag tgatacgacc acaatgattc caagtcccat aatgatcccc
     5461 aatgcctgct ttggcacata ggatgctttg gcactgtgaa taaacaaaag gctcatcgcc
     5521 atcagtacaa gtacgttaaa aacaagtttg aaattgtaat tctgcagtcg ataatttcgc
     5581 aacatagtat ctctccaatg ttattttcta agctcacgga tcggaatatt tgcatataat
     5641 gcaggtcctt cggattcttc gctactttgt gtgatctgaa tatcaagtcc ctctggatcg
     5701 atctccatat atttggagat taccttaatg atgtcatttt tgatcagttc catcgtctca
     5761 ggagagcaat ttgaccgatc tgaaacaagc agcaatttca aacagtcctt tgctacttct
     5821 cctgaactct ttttcttaaa aaaattatca aaaaaaccca tcgtttctct cccctctact
     5881 tcttaaatac gtttgcaagt ctctgaaaga aagttggtcc tgtgatttcc atgaatggaa
     5941 cgtctttgcc catcacacga ttacagatat tctggtatgc ttttcctgct ggtgtattgc
     6001 ttccaaccaa tggttctccc tggtttgtgg aaatgacaat attctcatca tctggtacga
     6061 ttccgatcag gtcaaccgca agaatatcaa gaacatcatc tttggataac atatctccac
     6121 gttctaccat atccatacgt atcctgttga tcaccagatc gatcttatgt atatcatttg
     6181 cctctaatag tccaatgata cggtctgcat cacggattgc tgatacctct ggtgttgtaa
     6241 cgatcagtgc acggtctgct gctgcgatcg cattcttaaa tccttgttcg attcctgctg
     6301 ggcagtcaag aatgatatag tcaaattcac tttttagttc ttctaccagt tttgacatct
     6361 gttctggtgt tacggatgtc ttatctcttg tctgtgcaga tggcagtaag aaaagatttg
     6421 gatatttctt gtctttgatc attgcctgtt tgattctgca gtttccttcc acaacgtcaa
     6481 ccagattata aacgattcta ttctcaagcc ccatgacaac gtccagatta cgaagtccga
     6541 tatccgtatc gatcaatacg actttctttc cttccttcgc taatcctgtt cccacattgg
     6601 cagtggttgt tgtctttcca acgccaccct ttccagatgt gattacgata acttcactca
     6661 tttttgtttc ctccattcac tatactatga tttcatttaa gagcgcttta gatatgggct
     6721 cgatataaat attttcatct tctacaaatg caatctgtgc ttccatcggc tgattgtcat
     6781 tattctttga tcttccgaaa agctttttct tcttctctgg ttctccatcg gcacttcttg
     6841 cgatgtgttt tccgatacgg atctgcatcg gctgcatgtc aagtgctgcg acaaaagcgt
     6901 gatcatctcc atcacatcct gcactcaaat atcctttggc acatccaaga acaactacat
     6961 tacctttggc acatacggaa gctccaggat tcacatcccc gaccactaca acacttgcat
     7021 cagattccag actctgtccg gagcgaagtg ttccacggta gaactgtcca tctccctttc
     7081 ttctctcatc aaccatggac tgacttcttt tttgttctct ttcctgaatc tttttcaata
     7141 catccttaaa agaagtttcc atgatcttat catcttctat cacatatgcg atcgtcaaag
     7201 ttgttttttc tgcaatgatc tgcagtacat tctgaagttc tttggcatct agttttctgc
     7261 cttcgatctt tagtgcgatc tgtgtctgtc cattaaagaa atcttttgcc tgcacaaatt
     7321 tatcttcgat cgctttgatc agttgatcaa acttcattcc tggatctaac accatggtaa
     7381 gtcccagact gtttcccttg agcaataccg gctgattcat acaattcctc tcttctttct
     7441 ctaatctcct gcagtaccaa cactggtttc tgtgactgat ttattattct tctctaatgt
     7501 cttctttgtc ttatcatttg caaaataata ctcataaata tcctttgcag tcgctgctgc
     7561 attagatgag gtatatccaa acggaataac aaccgttgtt gtgatctctg gcttctcata
     7621 tggtccataa gagataaaca acgcatgatt cggacgtttc ttattctcct gtgctgtacc
     7681 tgtcttacct gctaatttta ctttcatgcc ttggaagata ctgcttacgg agctgtcctt
     7741 tccgctgact acacctctca taccagtatg cactgcattc cagtatgttg tatcataatg
     7801 aagttgtctt actttcttaa ctccatattt cttgactgtc tttccattat cgtcagtgat
     7861 cttgtccatg atacttagat catatacggt tccactgttg gataatgttg tcacataatt
     7921 cgcgatctgg cttggcgtga aactattacg tccctgtcca atggcagaac gtacggatgt
     7981 ttcatcagaa acctgtggag atgactcatc cagttcaatt cctgactgct tattaagccc
     8041 tagctgtgtt gcgtatttct ccaagtactt aagaccttct ttactgctgt aggaacctga
     8101 tttgtctcct aatctatatc ctacttcata aaagaaataa ttacaagatg cttcgatcgc
     8161 tgttgacaca gttcttgctc catgacggcc tggatatttc cagcacttcg ccggtggtgt
     8221 gatcttatcg tataaccctg tacagttgat gactgtactt gtcgtgattg ctttctcatt
     8281 aagacctgcc aaagcagaca ctggcttaaa tgtagatccc ggtgctgtct tatgtttcag
     8341 tgcctgatta tataatggtg tagatgctga gtttgccagc tgcctgtagt atccggaatc
     8401 tgttccgttt gataaacggt tgctgtcata tccaggataa cttaccaatg cttttacctt
     8461 tcctgtcttg gaatctgtga tcacaacaga tccggaacat ggatccatac caatctgtcc
     8521 tggtgtgatc tgtaaactct ttaactttct tcgaatgaaa tcatagctgt ttagggaacc
     8581 agatttcaat gccgtataat cactgtcacc tttcttcttt aaaactccct gttcataaag
     8641 aaggagacat acttctcttc cgcttaattt tcctttcatg atgctttgtt cgcagactgt
     8701 catatcaaaa tcatctgcat caaccaaagc atcttctaca taagcagcca gtgctttcat
     8761 gatctcttca gtgtcattgt atttgctgct gatattcagt gaagaaatat caatccattc
     8821 cttgttcacg gcatacctta agaattcact taaactgatc ttctcattct tccacttctg
     8881 atatgtctta tccgtcgtat cgatagaact tgagatcaat actttcttgc tagacaacag
     8941 actgtataca taagatgcat aggcttgttt ttcttcactc tgatctttgt acgctttggt
     9001 atctttcaaa acaagattct ttgatacatt gatcgcctga tcttctaaag tctgaatctt
     9061 tcgatacatc ttcttctcat aggatgtcgc cttatctcca ttgagattct caaggtcgat
     9121 cacactattg ccaataaatg aataatatac tttcttgatc gggatcataa tattctcacg
     9181 gtcatttcct gcgctgtcag aacttgtaag cttagacaga acaattcccg cgatcttctt
     9241 ctcgagcaga ttatatacat attcctgtaa ttcgctgtca atcgataatg tgatattatt
     9301 tcctgttccc gatttgactg tcttggtcgt gtcgataatc tttccaacat tgttgacaac
     9361 cagagtcttg cttccgctgt ctccgtgaag ataattctca aactgcttct caacaccggc
     9421 ctttccaacc gtctcatcac tggaatagta atctttatct tcttctttct ttcctttgtt
     9481 atatgtttca agttcatccg tattgaccgt tcctgtatat ccgatcacat gagacatcgc
     9541 ctcactttta ttatatacac gaatagactt tgtatcaata tctattcccg gaagttctga
     9601 acttctttct ttgatctcgg caatacttct gttattgatt tcatttgcga tcgtcacctt
     9661 catatactga gaataacgac ttaaaaatgc actgtatctg acagacatga tctttaatgc
     9721 atctttcttg gaataagatt tatcaatatc aaacatcttt cctgttcctg cggagccaag
     9781 agttccattt ctcaaatatt caaaaacctg ttctgcagtt gcattcagct gtttatctct
     9841 taattctttc tcagactttg aaaggtctgt gctgtttcca attccaaaaa catctctctt
     9901 aaacttcttt aattttgacc cagtctctgt aaaacgaaac tttccgcttc ctgtatattc
     9961 aatcggaatc tcattgataa aggagtctcc atttcgctcc aggatcttga tcactttata
    10021 aatgacttta ttcttctcat aactttctga tgtaccattc tttgctgcca acgtctgaaa
    10081 tgcattgtca ttctggaaat tcactgtata tacaagtttg ttgtatgcaa gcagttttcc
    10141 attcacatca taaatattcc ctctggtacc agatgtcttg actgtctttt gtactttata
    10201 tgtaaaatca tttttatgtg actgaccttc tacgatctga aggacaaaca cacgcccgat
    10261 caacacacaa taaagcacaa cgatcagtat gatcaaggca agcagtctgt ttgccagtaa
    10321 tttttctgct ttctcgtaaa tttttcgtaa cactagaaca tactcctttt ttcaccatct
    10381 ctgatcctga tatcaacctc taacaatatc ttatatataa gcaatgctac gattcccgta
    10441 tatactgctt ccggaatcat aactttaaac agataagaaa aaaactgcaa tcttcctctc
    10501 attgcaaaaa agaaaaagaa gatcacaaaa ctatacgcaa gggaattcgc tgttagaagt
    10561 ccgatcggca gcaagatatc atcctcataa aaatatgtat ggaaccatcc attgacatat
    10621 ccaaacatta aatacacaaa tgcgttctgg cctaaaacat ttcctgagaa tatatcgatc
    10681 agcagcccac agacaaatcc gatcacgatt cctgctttcc tgcctcggat aaatgcgatt
    10741 gtgaccgtta agatcagtag aatattcggc ataacccctg cgagtttcaa aaaattaaag
    10801 acggatgtct gtaaaaggaa acagactagt accgatatca cataaaataa aattcgtttg
    10861 atcttcatat cctcacctgc tattcctttg tttcttcatc aagattcttt gttttcttaa
    10921 gatctgtgat aaccaaaact tcttttaaat gcttgaagtc aacgactggt gtcactttcg
    10981 ctgtcttggt aagctttgta gaatcaagct tcagatcaga tactttcccg atcgtgatcc
    11041 cttcattaaa tttggaactt acataagatg ttacaagctc atctccattc ttgacttttg
    11101 catctttgct gatatataca acttccagat atccatcttc catcgtatcc agacttccat
    11161 ttacgataca cgcatcattt gtctttagga acatagcact gaccatactt gtatcattaa
    11221 tgatcgttcg tactttggct gtatgtgatg atacatcata gacaattcct acgagaccat
    11281 tccctgcaat gacattcatg tctttcttaa tgccgtcttt gcttcctcta ttgatcagaa
    11341 agatgtcaaa ccagtttcct gatcctttac tgatgactct ggctcccact gtatgatatt
    11401 ttttgtattt ttgttttaat ttcaaaagat cctgcagacg ttctaattct acttgattct
    11461 gctgtagcac acgattctgc atcttcagaa catcgatctt ctcctgtaga cgctcattct
    11521 ctgctctcag tgtcttcttg cttttcgcat tctcattcca tgaaaacacg gcatttccta
    11581 ccgaattaat gccttcctgc atcggagata aaacagtgaa aaagatattc ttgactggag
    11641 ttaagactcc tccggcaaaa acactgattc ccgccagtaa cccacacacc acgataaaaa
    11701 tcgctaacag gtggtttgaa ttaaattttt tcttttgcaa tttcatacgt ctatcctctt
    11761 tcatcatcta tcgttttaaa tatgactgca ttcctaagat cacatgatct tctggccggt
    11821 cagcaatatg aaccggtatc ttaaattctt ctttgatcca ctctgccgtc tttggaaatg
    11881 ctgccccgcc tccagataca tagattccct gattcataat atctgatgca agttctggcg
    11941 taatgctttc taacactgct tttacctgca cacaaatgtc tctgacctga ctgtgggcct
    12001 tttcttcaac gatctgaatt ggtatcttct cttgttttgg aagtccactc acaagatttc
    12061 ttcccttgat catagctgct tctttatctt ctatcttcgc atatgcaagt tttaagtgtc
    12121 ttgcacttcg tgttccgatc gctaatttat aattcttctt tacataatct ctgatccact
    12181 catcgatctg gtctcctcca tatggaagaa gtctgcttgt tacgatccca cctaaagata
    12241 tcacagaaat ctctgttgtt gaagctccta tatcaatgat catatttcca catggcgcat
    12301 tgacatcaat tccgcttcca attgccgcaa ggattggctt gggaactaaa gttacatcct
    12361 taacccttga agctgattcc catgcaagat cttcaaatgc tcgtttctca acattcgtaa
    12421 tatccactgg aactgcgatc ataagaccgg ttttcctgat ccgtttgaaa taatggtcca
    12481 ataccgttcc taacagatat ctcatattcg tataatctga gatcacgccg tgttccattg
    12541 gtttcactac atgaatactg tatggctgtt tctcatacat cgcaaatgct tcgttgccga
    12601 atgcttttag tttcgcatct tcatcaaatg cgatcatatt ctttatcttt aaaaattctt
    12661 ctttatctct tgtacagatt cttaaatgtc tggttccaag ttcaattcct agttgtgccg
    12721 ttttcatgtt ctgctcctta catcatgtgt tcttctcgaa aacttaaata tgtctggtct
    12781 ccgatgataa tatgatcaca tagcggaatc ccaagaaatt ctcctgcttc tttcatccgc
    12841 ttggtcgtca gaatatcctg gcggcttggc gttggatctc cagatggatg attatggatc
    12901 ataagtacgg aaaccgcttc acatcgaagt gcttccagaa ataattctct tggtgatgcc
    12961 actgtctgat tcactgtacc aacagaaacc gtaagttcct ttataacttt attctttcca
    13021 ttaaaaaaaa ttaaaatcat ctcttcccgg cttaaatgcc gcatccgttc catataataa
    13081 tctgcaatgg atgctggtga atgaaagctt atcttatctt cgactttcgc acttgctaat
    13141 cgtttagaca attctgcaat acacaagatc tgcatcgcct ttactttccc aatcccggga
    13201 atcttttgta attcttctct tgaagtgtgg caaattccca gtattccctc ataaaacgga
    13261 tcgatcgaca acactttcgt tgcaagatcg atcgccgtct gatcctttgt cccacttctt
    13321 aagatcactg ccagaagttc tgcatctgac agcattgctg ttccattctt ttcacacttt
    13381 tcataaggtc tttctgacac tggtaattcc tttaatgtct gatgtttttc ttccatatct
    13441 gatcctttct gtcgaaaaat ctccatggtc aaaacatttc tcacattaaa tcttaccata
    13501 acttttcact gatgtataga aatcaaaccg ttgttttcct taaatttttc ttattttatt
    13561 ttatgaaatc tctggaattt ctaccaaacc tttttctgcc attcgctgca gggacatgat
    13621 gattccatat ttggcatgtg gatatgtaag acctccctgg aaatatactg cataaggctc
    13681 tttcattggt ccatcagcac ttaattcaat ggatgcgccc gatacaaatg ttcctgctgc
    13741 catgatcacc tggctatcat atcctggcat atcccatggg attggtgtta catgactgtc
    13801 gatcggtgat gctttttgaa ttccctcgca aaatgcgatc aatttttcag gatcatggaa
    13861 ttccactgcc tgaataatat catgtcggtc ttcttttcca tctggaatca ctttaaatcc
    13921 taatttttca tataaattgg ctgcaaagat cgctcctttg actgctgcat tgacaaccgt
    13981 tggtgccata aagaatccct ggataaactg acgattaact ccaagactgg ctcctacttc
    14041 ttttccaagc cctggtgttg taagtctgta tgcacactgc tcgatcagat cgcttcttcc
    14101 acagatgtat ccaccgatcg gtgcaagtcc tccacccgga ttcttgatca gggaacctac
    14161 gatcatatca gctccaactt ccgatggttc tttttcttcg acaaattcac cataacaatt
    14221 atcaaccatt acgatcaggt ctttattgat ctcttttaca aatgcgattg cttccccgat
    14281 cacatcaaca gataaggttg gtcttgtcgc atagcctttt gatctctgga tatggatcag
    14341 ttttgttttg tcattgatcg catttttgat cccctctaag tcaaagcttc catcttctaa
    14401 cagatcaacc tgtgcatatg tgatcccgta ttctttcagg gaccctttgg attcacggat
    14461 tccaatgact tcttctaagg tatcatatgg ttttcctgaa attgcaagaa tctcatctcc
    14521 cggacgtaag tttgcagata atgcaagtcc taatgcatgt gttccacatg tgatctgcgg
    14581 tcttacaagt gcatcttctg ttccaaaaac atccgcatac aatgcttcca cagcttctct
    14641 tccagaatca ttgtatccat atcctgttgt gccacccaga tgcatttccg cgatctgatg
    14701 tttctgcatc gcatttaata ccttgatctg gtttaactgt gcgatcgcat ctaacgtttc
    14761 aaaacgatct tttaatccct tttctacttc ctgtgcatag tcgaagactt tctcatcgac
    14821 gcccatttct ttataatagt tctttaactg atccatcata gatccccttt tcttttaata
    14881 ttgtaagcat atatgttaaa atctcatctt ctgacccaaa ttgtgattta tctaccatac
    14941 agacttcctt ttctcttcca aaccaggtca gctgacgctt tgcaaaatgt ctggtgtctt
    15001 tcttgatctg ttcgatcgta tcctccaaag aaagatttcc ttctaaatac tcaaacacct
    15061 gacgataccc gattccctgc atggaaacca ttgttttttc atatccttct tcttttaatc
    15121 gttttacttc atcgatcaat ccttgttccg ccataatatc aacgcggcga ttgatcctgt
    15181 catataagat atctcttttg tggtttaata caaaataagc aaaattatat ggtgaggtct
    15241 ttgctgcttc cttctcatta tgctctgaga tcggatatcc atttaattta taaaattcta
    15301 atgcacggat cacccgcttt acattatttt tatgaatctt ttctgtagat tttggatcga
    15361 cttgttttaa catctcgtat aaactgtcgt ttccttctct tttagctttt tcttccagtt
    15421 gttttctgta ttcctggtct ccatcttctt ttgtgaaatc aatatcatat aaaagacttt
    15481 ggatatagaa tcctgttcca ccaacgatta gtggaatctt gccttttgca tagatttcct
    15541 ttagggcatc ttttgccatc tgctgaaatc tcgctacatg aaattcttct ttaggatcaa
    15601 gttcatctac tagataatgc ttgatccctt ccatctcttc cggcatgatc ttggcagttc
    15661 cgatatccat cttcttataa acctgcatag aatctgcgga aatgatctct gcatctaaaa
    15721 tctttgctaa tcggatcgac agttctgttt ttccaactgc tgtcggtcct gttaaaatta
    15781 ccattggttt catctattta aaatcctttc ggcataagtt ctgataatcg atatgtttca
    15841 aatccatctg ggctttgaca gattactttc agatctggat tgaattcatg tagaaactgg
    15901 cggcagatcc cacatggata aaccgtttct ttgtcactgc cattggatgc gatcgcgatc
    15961 atttcaaagt catgatatcc ttcactgaca gctttgactg ccgctgttct ctctgcacaa
    16021 atggctgctc cataagaaga attctccaca ttacacccgg tgaatacttt tccttctttg
    16081 gtaagaattg ctgctcctac ttgaaatctg gaatatggtg cgtatgcttt ctcttttgct
    16141 ttatctgcaa gatctaataa ttcctgatct gtcatagtct tccctccttg tctggtttca
    16201 ctatacgatt ctcttaaact tcttgtctat ctcatactta ctcatcttga tgatcgttgg
    16261 tcttccatgt ggacaattat atggattatc aagcttcatc aaatccttca taagctgttc
    16321 catttccggc agactcnnnn nnnnnntcca tgtggacact ggtatggatt ttccagcttc
    16381 atcagttcat ctaaaagatg ttctacttcc attaacgata ctctctgatt tcccttgatc
    16441 gacatcttac atgccattgt cgcaatcttt gatgcgatca cttctgtatc caaggtacca
    16501 ttttcctgac ttaccgcatc taaaagttcc ataaataagt ctctttcccc gatcccatag
    16561 agattggctg gaacttcccg gatacaatat tcattccctc caaaagattc gatcgtaaat
    16621 cctaaacctg cgaatgcatc tttgtatctt gttaacgctt ctgcttccgt catagaaacc
    16681 gtcacaacca ttggcggttc taatctctgg ctgtatattt ctttttcttt gaaattcttc
    16741 atcagcctct cataattgac tttttcatgt gctgcatgct ggtccatgat aaataactgg
    16801 tcttcgtact caataagcca atatgtctta aagagctgac ctaagatctt gcggtcaggt
    16861 cttgcctgaa ctgaaaatac tggatcttct cttagtgtca tctgattgct gcttaattca
    16921 tcggtcttgt attgtggaaa ttcttttttc tgagtattgt agtttaagcc tgcataagtc
    16981 ggtttctttg tattttttga tgattcgctg gctgttagtg ttttctttac aggatgtgac
    17041 ggcttttcaa tctgttgttg tacagcttgc ctttgctttt caacctgctg ttgtacacct
    17101 tgcccttgct gatctacctt ctgaacctct tctttccttc ggttcacctc aaatggctca
    17161 ggctttttca cttcttttgt cttcggcaat tctttcttct tcggtgtctc tcttaataca
    17221 tcctgcacca gatctctctg aagtaatgcc gctctgactg catctactac aagctcgtag
    17281 atttctcttc catttcggaa acggatctcc atctttgtcg gatgaacatt cacatccagc
    17341 agttcctgat cgatcttgaa atcaaggcac acaaatggat atctgtgttt catcgtaaag
    17401 gtgcggtatc cttcttcgat tgccttgtaa atgatcgagc ttttgatata tcttccatta
    17461 ataaagtaac tctcatagtt tctgtttcct ctggaaacat acggttttgc atcttcattt
    17521 tgtgcttcta gcgggatcaa tgctcctgcc acatctcgcc cataaatatg atagatgatg
    17581 tcctttacct taccatttcc agaagtataa atcttatttt tattgttatg gataaattta
    17641 aatgaaattt ccggatgact tagggcaatg tgtaccatca tctgttctac ataccctgct
    17701 tcggttgtcg cactctttaa gaattttctt cttgctggcg tattataaaa cagattcttt
    17761 accagaaatg ttgttccatc gggtgctccg atatcttcaa aagcttcttc ctcgcctcca
    17821 tagatcttat aggatactcc gctgaaactt tctgcagtct tcgttaccag ctcaacctgt
    17881 gcaactgcgc tgatacttga taatgcctct cctcgaaatc caagcgaaga tacacttaac
    17941 agatcttcaa ccgaacggat cttgcttgtc gcatgacgta aaaatgcagt ctttacctga
    18001 tcttttggaa ttccaatccc attatcagtt accctgatca atgtcattcc gccgtctttg
    18061 atctccacag tcactgcatt tgcttttgca tccactgcat tttccacaag ctctttgaca
    18121 acagaagctg gccgttcaat aacttctcct gctgcaatat tatcgatcgt cttttgatct
    18181 agtaattgta tcttcgccat aaaattactc ctcgttcttt aacttctcct gtaacacata
    18241 taaataattc aatgcttcca gcggtgtcat gtcagaaaga tctttttctc ttagttctgc
    18301 cataaatgga tgttccatcg ttggttcatc gctttctctg aagaaactta actgttgtgc
    18361 ctctccatat ggcatgatat ccttcgctcc tgacgtcaga tcatgttctt ctaattctct
    18421 tgcgatctct tttgctcgat tgatcacggc atctgggact ccggcaagtt ttgcaacctg
    18481 cactccataa ctcttatctg caccaccttt tacgatctta cgcaggaaca cgatgtcatc
    18541 tccctgttct ttgacagcaa tgcagtagtt atgtacactg tcgatcttac cttctaactc
    18601 tgtcagttca tgataatgtg tcgcaaacag agtttttgca ccgaggagct tggaattgct
    18661 gatatattcc acaactgccc atgcaatgga caacccatca aatgtactcg ttccacgtcc
    18721 gatctcgtct aagatcaaca aactgttttt cgttgcattt cttaaaatat tggcaacttc
    18781 actcatctca accataaatg tactctgtcc agaagcaaga tcatctgatg ctccaaccct
    18841 tgtaaagatg cgatctacga taccgatatt tgctttcgcc gctggtacaa aggaaccaat
    18901 ctgtgccatt aatacgatca gggcaacctg cctcatataa gttgatttac ctgccatgtt
    18961 tggtcccgtg atgatcgcta cacgattctt cttgttatca agatatgtat cattagaaat
    19021 aaacatatca tttggaatca cctgctctac aaccggatgt cttccatcct tgatatcaat
    19081 cgtccctctg acatttaact tcggacgtac aaaatgattg cgttctgcga catatgcaag
    19141 ggaggcaagt gcatcgatct gcgcgatcac attcgctgtt cttgagattc gttccatctc
    19201 tcctgctaat gtttcacgga tcataacata cgtttcatat tctaacgcat acagacggtc
    19261 ttcggaaccc aggatcgtat ccgccagctg gtttaattct tcggttgtgt aacgctcgga
    19321 attggccagt gtctgcttac ggatataatg atcaggtaca agatctttgt aggaatttgt
    19381 cacatctaaa tagtacccaa atactttatt aaacttgatc tttaagttct tgatccctgt
    19441 tttttcacgt tcccgttcct caagttccat caaccattgc tttccttctg ttttagcacg
    19501 ttttaatttg tctatatctt ccttgaatcc ttccttgatg atcccacctt ctttgatcgc
    19561 aagcggtggt tcttcaatga tggaatcttc caaaagattg tggatatctt ccaatggatc
    19621 taaatcttct cttaatccag acaataatgg atctttgcat tcttcaagaa ctgttttgat
    19681 cgcaggcagc aattccaacg atgttttaaa tgcgatcata tctcttggat tcgctgtctt
    19741 ataacttact tttgtcatta aacgttccag atcatagatc ggatttaagt attctcttaa
    19801 ttcttctctt acgatcgtct gatccgtaag tgttgtgatc gcatcgtatc tctcttcgat
    19861 ctttttcttg tccaccaatg gctgctcaac catgtttctt agcattctgg cacccatggc
    19921 tgtttttgtc ttatctaata cccataagag tgaccctttt ttttgtttat cacgtaatgt
    19981 ctcacaaagt tccaaatttc tacggctgga gctgtcgatg atcataaatt cacttgtctc
    20041 ataagcttca atatgcatca gatgatccag agaactcttc tgtgtctcat gaagatatcc
    20101 aagcaatgca cccgttgcga tcactcccat tggatgatcc aaaagtccaa gaccttctaa
    20161 atttcctact ttgaactgac gcttgatcct ttcctctgcc tgttcttcat caaaatggta
    20221 tggctcaatg cttgagatca cgatcgacat cttgtctttt aagtaatcaa aatccatgcc
    20281 actgatcaaa aatgcatcat tacaaacgat ctctgccggc tcgaacttga agatctcatc
    20341 tctgactttc gctaaactat ccattgatgt tgttctaaaa tcccccgttg taacatcaac
    20401 aaatgaaatt ccgtaacttc catcatttgc aaaaatacac atcagatagt tatttttctc
    20461 atcatcaaga ctctgctggg aaatattcgt tcctggcgtt acaatacgca cgatctctcg
    20521 ttttacgatt ccctttgcct ggcttggatc ttcaacctgc tcacagatcc cgactttata
    20581 tcctttttct actaatttat ttaaatagga atccacagca tgataaggaa cgccacacat
    20641 tggtgcgcgc tcctcaagtc cacagttctt ccctgttaat gtgatctcta attctttgga
    20701 tgctgtcagt gcatcatcaa agaacatctc atagaaatct ccgagacgat aaaataagat
    20761 acaatcttta ttctcttctt tgatctgcat atactgctgc atcattggtg tcagttttcc
    20821 ttttttagcc atggatctgc tctcccatat aatagaaacc tttttcctca actaatttaa
    20881 catctgccat tgtaccaatc tgttctttgc atcctttaaa atgaactaac aggttatttt
    20941 ccaatcttcc tgttaacatt ccttcttcgt gcgtattctc ttcttcgatc agaactctct
    21001 cgatatctcc gactttcttc ttgcaattct ttgcagatgt ctcttttaat aaggcaagaa
    21061 gtctgtcaaa acgatctttg atcacatctt tgtccacctg atcttccatg ctcgctgctg
    21121 gtgttccgga acgtttcgaa tatacatatg tgtatgcact gtcaaatcca acttctctta
    21181 caacatctaa tgtctccttg aaatcttctt ctgtctctcc agggaatcct acgataatat
    21241 ctgttgtaaa agacactcca gggattctct cctgaatctt cttagcaagt gccacatact
    21301 gttcttttgt ataatgacga ttcatcaatt ttaacaggcg gctgcttcct gactgcattg
    21361 gaagatgcat atgtttacat actttcttgc ttgttgccat cacttcgatc agatcatccg
    21421 ataaatcctt tggatgtggt gtcataaaac ggattctttc tagtccttcg actttctcta
    21481 cttctcttaa taattcagca aatgacattg gttcttctaa tgtctttcca taagaattta
    21541 cattctgtcc aagaagcatc acttctttga caccttctga tacgacctgc tttacttctt
    21601 tgatgatatc ttctggctta cggcttcttt ctcttcctct tacatatggc acgatacagt
    21661 aactgcagaa gttattgcat ccatacataa tattaactcc acatttgaat gagaatttac
    21721 ggtcacttgg aagatcttcc acaatctggt ctgtatcttt ccagatatca acgatcattc
    21781 ccttggagtc tactcttgct tttaataatt ctgctaattt aaagatattg tgtgttccaa
    21841 agatgatatc tacaaattta tagcttgaac gaatcttctc gatcacatgc tcttcctgca
    21901 tcatacatcc acataacccg atcatcatct gtggatttct ctctttcatc ttcttaacct
    21961 gccccaaatg tccatataac tttgtattgg cattctcacg aactgtacat gtattaaaga
    22021 tcagaaagtc tgcttcttct gtttcaactt cttcatatcc gatcgtttct aagatcccta
    22081 ataacttttc agagtctttt tcattcatct ggcagccaaa tgtggttaca tggcatgtga
    22141 gcttccttcc caattctcca gactttttct gaacccattt tttcatttct tccatatatt
    22201 catactgtct tgacgactcg ttctttaact gttccattct attatttctc ctactttata
    22261 taattcatct ctatttttac agtatctttg attataacat gaatgttctg cttagacaat
    22321 tttaattttg tggcaactac tcttcaacca aatttttcga tatttttctt gtcacattgc
    22381 ttcgtttaaa gtaaaatata agtaatattt atgttaagtt attgttaaga aggagaactg
    22441 tcgtatgaac tatactatca tacttgctct gatgggaggc cttggcctct ttctttatgg
    22501 gatgaaactg atgagcgatg gacttgagaa agctgccggt gcgaagcttc gccggattct
    22561 tgaaatattt actacaaacc gtttaacggg tatgttagtc ggaatctttt ttaccgctgt
    22621 tattcagtcc tctagtgctg ctactgttat ggtagtcagc tttgtaaatg cagggcttat
    22681 gaacctgtct caggctgctg gagttatcct tggtgctaac attggtacta ctgttacttc
    22741 ccagcttgtt tcttttaacc tttctgagat tgcccctgtc tttttaatgg cgggtgttat
    22801 tatggtatta tttatgaata atcctaccgt ccagaaagtg ggagaagtta ttcttggatt
    22861 tggtgtttta tttatgggat taacttccat gtcaagtgct atgtctgtgt taagggattc
    22921 acctttgatc acatcttacc ttcagacact ggataaccat ttccttggtg ttttatttgg
    22981 atttatcatg actgcgatct tacagagttc ttctgttact gtaagtatcg ttcttttgat
    23041 ggcatctcag ggattattac atcttccaat ctgcttcttt attattcttg gatgtaatat
    23101 gggatcttgt gtgtccgcat tgcttgccag cttaagtggt aacaaaggtg caaaacgtgc
    23161 tgcgatgatc catttcttat ttaatgtatt tggaagtatc atcatcttta caggacttag
    23221 ctttgcactt actcctatca caaacttttt cttaagtatt tccggtggaa atcttggacg
    23281 aagtgttgca aatgctcata caacatttaa gatcattgaa gtattattta tgttcccatg
    23341 tacaccttta gttgttgcat taacagagaa gatcatccgt ggaaaagatg aaaaagtcca
    23401 ccataatgaa ctgcagtata ttacagagat ttccttaaaa tctcctgcaa caatgatccc
    23461 tcaggcgaag aatgagttaa gacgtatggc taatatggca caggagaatt tagaccatgc
    23521 catccacgga tttctaaatc aatccgatga atttgttgat aaaatctatc atcgcgagga
    23581 agatatcaat aatgtcagtc atgcgatcac tgattatctg gtaaagtcca accagctatc
    23641 actgccttta gccgatcaga agatcttggg ttccatgttt tatgttgtta atgatatcga
    23701 acgtatcggt gatcacgcag aaaattttgc tgattttaca aaaacagaga tcaaacataa
    23761 cactggactt actggagatg caaaagaaga aatcaaaaag atgtacactg ctgtttccaa
    23821 actgttaaaa ctttccctgg agtgtttcat ggaacaagat ggtgtcaaca aacctgaaga
    23881 aaagcttgct gagattgcaa ttctggaagc ttcgattgac aaaatggaac gcagatatca
    23941 aaaacaccat atcaaacgtc ttgccaaagg cgaatgtgag ccaagagctg gtttattgtt
    24001 ctccgatatg ctttctgaat tagagcgtat tgctgatcat agtgtgaata ttgcttattc
    24061 catgagtgat gaagatgaag atgaaatact tgctgctgag aatgaagccc tcactgctaa
    24121 aaattaaatt tgagttgaac ctagaatata agaaaaggat ctgcaaaccc ttttgatact
    24181 ttatgtatct ttgatctgca gatccttttt cttttttgct tttataactc tatcacttta
    24241 taatctatag gtgctacatt tgtcttgatc acagcgttgc tcaagttctt taacagcgcc
    24301 tgtaatgcat tctcattgac cccttctttg accttgttga gatctagatt gatctcttct
    24361 tttttcatcg tcggattgac gagaagtaat attgctttgt tctcacttgc ttctccaaag
    24421 acatcttttt tctcgctgat aaatcttaag atcgcaagga cattttcagt cggtgccata
    24481 tataatgcat atcctgtctg taagactttt tcacggtttc taaggctagt cagtctcaaa
    24541 acttcctgat aggtttcttt gtcattttca aaaaatgctc ctcggttaaa tgggtccatc
    24601 agtccatgca ttccatattc gtctccataa taaatgcatg gcattcctgg gacaaagaac
    24661 tgaactgcca ttgcaagtct tgtgagtgct tttcctttct gatccatctt tgatgttata
    24721 acatattctg cctgctgctc tctcgatggc agattctcat tcattccagt tgcaagtgtt
    24781 gtccgaattc ttggaatatc atgagaagat aacaggttca tcagtgcata atacaatggt
    24841 tttggataat tacactgctg ggatatcaag aatgtcttca tatctttggc actttgatta
    24901 ccgagtaaat aatctgtaac gttgactttc agcggatagt tcattgcgct gtctaagcct
    24961 ttacccaaag catatttacg tttcgttccg taactttctt tggttgtgac atcttcccat
    25021 acctcaccga tcagtgcata attcttgaat ttcttcttta attcgcttcg caatttaaat
    25081 ataaattcat ctggaagttc gtctgcaaca tccagacgga atcctgatgc tccaagtttt
    25141 aaccaatgct tgatcaccga atcttctcct gtgataatgt aatcaacaaa cttttcattt
    25201 aattcattga cttccggcag tgtttcaaac ccccaccagc tcttataagt tccatcttcg
    25261 ttaaagtcat accaatcata gtactttgac tcttcccctt gatatgcccc tggttcttcg
    25321 taatttccct ttttgttaaa atagacacta tcatccccag tgtgggaata taccccatcc
    25381 aagatcacat gaatcctgcg ttctttggca agctggcata gccgcttaaa atcttcgtta
    25441 tttccaagga atggatcgac tttcttataa tctgatgtat tatagcggtg gtttgatgct
    25501 gcttctccaa tcggattcag ataaatacaa ctgatcccca atcgtttcag atcatcaagt
    25561 ttggatatga ttccctcaat atctccacca aaataatcac atggatcata aaattctttt
    25621 cctggaagtg gtccaaatac tggctgttct tcccagtcat catgcagata gaccgttctt
    25681 cccatacttt catgatatac tttcccgcgt ttcatatttt ctttgtttcc ctggcagaaa
    25741 cgatctggga aaatctgata catgattcct tttgacatcc accttggggt atcaaaattt
    25801 tcttcatata cggtgatctg aaaactatcc gggttgtcat caacgatcat tccttctcct
    25861 tgtgtcttcc catgtctggc tccataaaaa taacatttcc catgttccca gattctgaaa
    25921 taataccaga caacacctgc ttcactggtc atctttatgt gttcttccat gcagtctttt
    25981 tttcgtttca tctcatactc ccggtgaaaa ctttcagaat acatgatgat ctctgcctta
    26041 ttgacttcct gccctacatc atacaggcgg atcatcatct cgcttccaca tgtgactgca
    26101 cctacgggag ttctgtattt ttctactgac gcgttatgaa tcataattta atctcatctt
    26161 ttttgtaact cttattttac tggtttcaga ttccagattt tttcattata ttgttcaatc
    26221 gttcggtctg atgaaaaata tccggatttt gctgtattaa caattgcttt tttaatccat
    26281 tcatccttat gatcgacata caaatgatat actttttcat atgtttcaat ataagacgga
    26341 aaatctgcaa gtatgtagta tggatctgcc tctccatatt ccccaaataa aagattctgg
    26401 taaatctctg aatattttct gtttccgtat cgtaacagat ttccttcgat cagacaatta
    26461 catacatcac ggattcctgg atgattctcg aaaataatcc ctggattgta tgtatggtac
    26521 atttttttat tgtaattacc ctcagcgctg tccccgaaga taaagatatt ctcctgacct
    26581 acacgctctg cgatctctat attggctcca tccatagtgc cgatcgtcaa tgcaccattc
    26641 atcataaatt tcatatttcc tgttccagat gcttccattc ctgcggtaga aatctgctca
    26701 ctgatatnnn nnnnnnncgg aatcagcatc tccgcaaccg atacacaata attttctacg
    26761 aaaaccacct gaatctttcc gtccatatct ggatctttat tcaccatttc ctgaatggaa
    26821 tggatcagcc ttatgatctc ttttgccctg gcatatcccg gtgctgcttt cgccgcaaaa
    26881 ataaatgtga tcggtgttgt aatacatgct ggatcttttt tgatcttctg atatatataa
    26941 atgatatgca gacattttaa cagctgtcgt ttatactcat gcaaacgttt gcaatgaaca
    27001 tcaaaaatag attctgggtt cacttcgatc ccttgctttt ctttgatata ctttgcaaaa
    27061 cggatcttat tatttcgttt gaccttctca tattttttac aaaattcttt gtcatcttta
    27121 tatggaagga tctgttcaaa cagacggtaa tcctttaaaa tatccccttt cacatactct
    27181 cttaccagat gatatagccc ctgattggcc agtgctaacc agcgtctgtg tgtgatccca
    27241 tttgtgatcg caagatattt ttgtggaaag atctgataag atcctttaaa aatcctcgtt
    27301 tttaaaatat cagcgtgaag atttgaaaca ccattgatcc tgcggcaaag agctacgcat
    27361 aggtttgcca tgcggatctc attgtttccg atcactgcca tctgtgcgat cttttcttct
    27421 tccttgctgt aatatacgga aagtttctga caatatttct cattgatcgc acaaatgatc
    27481 tgatagattc ttggaagcag taaacgaaac atattctcat cccaacattc cattgcttct
    27541 gacataattg tatgattcgt ataatgaaat atcttcttga ccataccgta ggcctcatcc
    27601 catccaaaat cttcttcatc cattagaatc ctcataagtt ctggcatcgc caatgccgga
    27661 tgtgtctcat tgatctgaat gacaacatgt tctggcaagg agttcagatc atcaaatact
    27721 gctttatgtc tcttgatcat cgactgcatc gttgcagatg ccagaaaata aaactgtttt
    27781 aaacgcagca ttttaccctg catatgatca tctgacggat ataagacttt cgagattgct
    27841 tctgcaaatg tctggtctgc catcgcttta tcatagattc cttcattaaa cgaatgaaaa
    27901 tcgaaccgcc gcttgcttct ggcactccac agacgaagag ttgctggcgt cttagtcttg
    27961 tatccaatga tcggtacatc atatgggact gcaatgacgg tattataatc tttatgttca
    28021 atctttaatc cattctctgt ccagttttcc tgaatcgttc cattaaaatg tacttccaca
    28081 cttaattccg ggcgctcgat ctcccaaaca tttccatctc ttaaccagtc atcttccatc
    28141 tcaatctgcg ttccatctac aattttctga cgaaacattc catattcata tcggatccca
    28201 catccgagta ctggaagttc catcgtagaa agtgaatcca aaaaacatgc tgccagtcgt
    28261 cctagtcctc catttccaag tcctgcatca ttttcttcat ccgtgatctt atcaaaatca
    28321 agtcccatct cccagaatgc ttcccgatat gcctcatata atccttgact gatcagatta
    28381 ttagaaaatg cccttcccat caaaaattcg gctgacatat agtaaagttt cttaactccc
    28441 tgctcttgta ccctttttct gctgtctgcc caggatatca tgatatcatc cctgatacac
    28501 tccgctgctg ccttatagat ctcatcattt gttgcctcac tgatcgttct tccaagtgca
    28561 tgaattaatt tattctcgat ccgttgtccg atcttattgc tgtccatata gtccattgtg
    28621 atctaagcct cctttctagt ctttatcttt gacctgtaaa tttatctcat tttttgtctt
    28681 tatgaagaat gggcatctta aaataagatg cccattcttc ttttgaattt ataatacact
    28741 attatacata tcaagataaa tctttgcaga ttttgcaaaa ctgttatcgg attccatcgc
    28801 tctttggatc aatccatgtt ttctctttgg actctgcaat gtctgtaatg cataacggat
    28861 tgctcccagc atctcatgtg cattgtagtt tgcaaatgag aatccatttc ctgtgtcctc
    28921 gtattcattg taactttgta ctgtatcacg aagtccacct gtctcacgta cgattggaag
    28981 tgttccataa cgaagtgcga tcatctgaga gattccacat ggttcaaatt tggatggcat
    29041 taagaagaga tcacttcctg cataaatctg gtgtgcaact gcattatcat atgcgatata
    29101 tgagcatact cttcctttgt atttttcttc catgtaacgg aaaaattcct catattcata
    29161 atcacccgtt ccaagaatga caaatgcgac attctctgta cacatgatct catccaacat
    29221 gtatttcaca agatccagac ctttctgctc tgttaatctt gtaaccatac cgatgatcgg
    29281 aactcctggt gtgatcgata atccaagagc cttgatcagt tcctgtttat ctttcttctt
    29341 tccagaaaga tcatctttgt taaaatgata tggaatcaat ggatctgtct ctggattaaa
    29401 ttcttcctga ttcattccat ttaagattcc ccatgtatct cctgctctcg catttagttg
    29461 gacttactgt gcttaaacga tctgcaaaca caagtgctgc tttcatgaaa cttgcacatt
    29521 catagttatg cataaattct ggtgtgtcat actttggatc gattcctaac caatgttcaa
    29581 actgatcgaa accaaattct ccttgataca tcatattatg gatcgtaaag atcgtctttg
    29641 catgtccaat atcccaatgg ccgtactgtg tgcggagtaa gatagggatc agacctgtct
    29701 gccagtcatt gcaatggatg acgtctggaa taaatccaat acggcttgct gcttcgatca
    29761 cggcacggca gaagaaagca tactgctctc cttctgcaag tccaccacga tataccgcat
    29821 caccaaaaaa tccttcatta tcgatcagat aatatgtaac ttcattataa accagttctt
    29881 ctacacctac atatgtttcc ccaccactaa aattgatgtt gaaatctgcc agatgtcttg
    29941 tctgtgcgcc atatttccct ttcatctgac tatgaaacgg catgatcaca cgggcatccg
    30001 caccgatctg attcaattcc tttggaaggg ttcccataac atctgctagc cctccaagct
    30061 tcacaaatgg tgcacactct gcccctgcaa gcagcacttt cagtgaattg ttattactca
    30121 ttaacacttg ctcctttcgg aataaatact ggatggtttg atgatcctga taagtgtgca
    30181 tttgaataaa cttttacgtg tcggtctaaa attgtatggt tgatctctgc ctgtggtgcg
    30241 ataaatcctt ctgccataac aacagaattc ttgatcacag ctccctctgc aatctgtgat
    30301 ccacggaaga tgacagagtt ttcaacttca ccatcaatct cacatccact actgatcagg
    30361 ctgttgctga ctttcgcatt tggtccgtat tttgctggtg ctccatcggc agattttgtg
    30421 taaattcttc tattacccaa gaataattct ttgtaaacat ctggttttaa catatccata
    30481 tttacattgt aatatgcttc taaagatgta atacatccta catatccttt atattcatat
    30541 ccctgaatta caagacgatc gaagtttgga acgataacat caaagaaaaa gtgtttctta
    30601 ttatgtgcga tcgctgtctc aataaaatcg atcaataatg ttgtcttcat gatataagca
    30661 tccatgctct tcttatttga acgatgtttt ccttcagaat agcacatatc aagaacttta
    30721 tcgtcttcat ctgtttttaa ataaactcca tcataatcgc agttcgttga atcatcacta
    30781 ttatcaaaat acatcagtgt gatatctgct ccagatttga tatgtttatc taacatatcc
    30841 tgaaaatctg cattacagat cacataagaa cgtcctaata atacatattt ttcttttgct
    30901 ttctttaaaa atccaagtgt tccacgaagt ccatccataa cacttgcgta ttctccggat
    30961 ggagatgcat cattaatata tggtgggaag atatataatc ctccacgttt tttgtttaat
    31021 ccccagtatt ttcctgaatt cacatggctc aacagtgaat cgtaatttct tcttgttaat
    31081 aatcctacat tcttgattcc tgcatttacc atattactta acataaagtc aactgtacga
    31141 taacgtccac caaccggaag tgctgataaa cttctggagc gtacaaggtc ttttaatttt
    31201 ctattttctt ctcctgcatt gataatacct aacatctgtt tatccatatc ttcaatcctc
    31261 ctagtgtaag atggtatctg ggtagatcat tgctccgtct tcaacagtcg cattctctaa
    31321 tgcaacacaa tgatatccga ttacagttac attatttcct tctccaacct tgcatccctg
    31381 ctcgatgact acttcttcat cgatcagtgc tttgtcgatc tgacagtctg ttcttacaac
    31441 agtatctttc atgatcacac tgttcttaac tacactgcct tttccgatct taacaccaga
    31501 atggatgaca cagttctcta cttcaccttc aataacacat ccatcggcaa gaatagaatt
    31561 cttaacagat gcacctggtg ctatataatg aggaggtaat gagaagtttc ttgtgtagat
    31621 tctcatctta tcatcattta agtcaatctg aggattttca tccagaagtt ccatattagc
    31681 ttcccataaa ctgtcgatcg ttcctacatc tctccagtat ccatcaaatg agtaagcata
    31741 catctttctt ccatcttcta acattcttgg aataatgttc ttaccaaaat cgtgttctga
    31801 ctctggatca tttccatcat cgatcagata ctgacggatc agtttccatt taaagatata
    31861 gatacccata gaagcttttg tactctttgg ttctgctggt ttttcttcga attcataaat
    31921 acgttcatct tcatctgtat tcatgattcc aaaacgtttt gcttctnnnn nnnnnnnnnn
    31981 nnnnnnnnnn nnnnnnnnnn nnacattcag aactgagatc gtacaatctg cacctttctc
    32041 tttatgatac tgaagcatct tgtcataatc catggtacag atctggtcac cagataatac
    32101 aatgatatga tctggatcaa aattatcaat gaaattgatg ttctgataaa tcgcatctgc
    32161 cgttccacgg taccagttac ctgtttctcc cttcatatat ggaggtaaaa cataagcacc
    32221 tccgtttaag ctgtcaagat cccagaatcc tccagtacca atatatgcat ttaactccag
    32281 tggttcatac tgtgttaaaa ctccgactgt gtcaattcct gagtttgtac agtttgacag
    32341 tgggaagtca atgatcttat attttccacc ataggatacg gctggttttg ctttcttctg
    32401 agttaatgcg aacaatctgc ttccttgtcc ccctgcaagc agcatcgcaa cgatttcttt
    32461 tttgttaatc atagctcttt gtcctccttt attttacacc ttgatcatta ttcttttttg
    32521 ttcttcttgt cttgcttgtt cttttttttg gctgttcctc ttctttctct gttgtttgct
    32581 ttgttacctt tatatcttct ttttttggag tgtctttctt agtctccggc gcctcttctt
    32641 ttgttacttt ctttgtaact tccggctttt tctctactgt tttcttcaca gcttctgttt
    32701 tcttctcctg tgccgccttc tttgctggct ctgctttctt tgcagtggct ttctttgccg
    32761 gctctgcttt ctttgctaca ctcttccgtg gtttcgtctc cacttcctcg atcttatagt
    32821 aaacaacact taatggtttt aaggaaattt ctgctgcctg ttggtaaaca gctttctttc
    32881 cttcagatgg tactttcacc ctcttcgttg aaactttaga ttcggaaaca tctccatctc
    32941 caccaaaatc aagctcactg ctgtctaaga tcttcgtaag ttttgatttc tttggtaatg
    33001 gtaccttgta tttatcccat tccatcggtg taaaattgca gacacaaaga atctgttgtt
    33061 gttttcttcc tctgcccttc ttattctttc gtataaatgc tacaacactg tggtctgcat
    33121 cacgttgcaa gcaccattca aatcctgctg gatcctgatc taattcatat aatgccggtt
    33181 cagatttgta aatctcattt agtttagcta catatttgtg gaaagaatca tgcatctcat
    33241 actgatcgat caagaaccag tcaagctgtt ctttgtctct ccattctaca aattgggcga
    33301 attctccacc cataaataat aatttctttc ctggatgtgc catggtaaat ccatagttcg
    33361 cagtgaagca aacttctcat catatgcacc atacatctta ttgatcatag aacctttgcc
    33421 atgaacaacc tcatcatgtg aatatggaag aatgtaattc tcactgtaag cataatccat
    33481 agagaaaatg atcgctccat gattatcctt acgatacaat ggatcaagtt ccatatagta
    33541 aagagtatca tgcatatatc ccatattcca cttgtaaaca aatcctagtc cttccggatc
    33601 atcaacatca cttgttacct ttggatatgc tgttgattct tctgcgatcg tcaagaatcc
    33661 ctcatgtgtt gtacgaagtg cttcgtttag attgcgaagg aaatcaactg cttcatagtt
    33721 gatattggtt ccatcttcat tcggcacata ttctccttcc tgtttcccaa agtcaagata
    33781 tagcattgca gatacagcat caatacgaat tccatcgata tgataaacat ctgcaaagaa
    33841 catcgcactt gagatcaaga agctctgtac ttctggtttc ccatagttaa ataataaagt
    33901 tccccagtca ggctggcttc ccttccttgg atcctcatga tcataaatat gtgttccatc
    33961 aaacattgcc agtccatgct cgtctcttgg aaaatgtgct ggaacccagt caaggatgac
    34021 actgatccct gctttatgta actcattgat catataattg aaatcttctg gtgttccata
    34081 tcttgatgtt acaccgtaat atcctgttac ctgatatccc caagaaagat catatggata
    34141 ttctgtgatc ggcatcagtt caacatgtgt atatcccatc tctccaagat aagggatcag
    34201 catgtccgcg atctcacgat aattataaaa ttctctctct tcttcctgcg gcattctcca
    34261 tgttccaaga tgcagctcat agatagacat tggctgatct aatgcctggc gtttcttcct
    34321 ctgattgata aaacgtttat cagaccattt aaaatcatcg aaatcccaga ctttcgaagc
    34381 tgtatctggt ctgcattctg cataaaatgc aaaaggatca ctcttatatc tgcagattcc
    34441 atcatagcct tctacatgat atttatatag atcacctttc ttcagtcctt caatctcgat
    34501 cgtatagatc ccgtctttgt cacctttcat cggatgcgca agaggattcc agaaattgaa
    34561 atcacctacg acactgactg actttgcatt tggagcccaa acagtaaatc tgtgagtctt
    34621 tgtcttcttc tgataatggc atccaaatgt caggtatgcc tgccttgcct ttcccgtctg
    34681 aaacaaatac aaatcctcac gattgatagt ttcattcata gtataactcc tccttaaaac
    34741 ttcaaaacaa agaattcttg tgattgaatc ccacacttac agaaaatctt atcttataag
    34801 acttctttgt gaaactaaaa agtctaataa aataaaaaag cgtaaacaac cacgaacaat
    34861 cttcttgcaa atggcttatt tacgctttta tagtcatatc tttaatttct ataggtataa
    34921 taaaaatgtg acacgttgtc atcttacaag atattttcat attcaaagaa caatgtactg
    34981 taaatatctt tcgtttctag tccactttct ggttgtttat ctgctgttca aaaagtgttt
    35041 gtttcactaa ctttattata ccaaatcttt tcatatattt caacaaattg tacaatattt
    35101 aatgcactat ttatatccat attgtgaaat tcatccaagc aatttaatat acagatgtag
    35161 aatttctcat tttaggatca tatcctgctt ttttcaagga tttaatgatc ttatttttat
    35221 gttctgtacc gaatgcttcc attgtgatcg taagttctac cgcactattt ctgttgatac
    35281 ttacgaactg gttatgctct agtttgataa tatttccctg tgcatctgcg atcacatcag
    35341 aaactttatt taactctcct ggtttgtctg gaaggaatac agataccgta aagattcgat
    35401 ctctcatgat caatccatgc tgtactaaag atgcgatcgt aatgacatcc atatttccac
    35461 cactgatgat agaaactact ttcttattct taacatccag atgttttaat gctgcaattg
    35521 ttagtaatcc agagttttct gctaacatct tgtgattttc taacaagtct aagaaacatg
    35581 ggatcagttc ttcatcttct actgtgatga tatcatcaag gttttcctga atatatggaa
    35641 aaatgatctt tcctggtgtc tgtactgctg taccatctgc gatcgtgctg acatgatcac
    35701 atgacactac ttttcctgct tctaatgaag ctttcataca tgctgctcct gctggttcta
    35761 caccgatcac tttaatcttt ggatttaaca gtttggcaag tgttgaaaca cctgttgcaa
    35821 gtccgcctcc accgattggt acaaggatgt aatctacggt tggaagttct ttaaagatat
    35881 ccattgcgat cgttccctgc cctgttgcaa ccgttgggtc atcaaatgga tgaacaaatg
    35941 tatatccatg ttctgctgca aggtctaatg cgtgtgcaca tgcttcatca taaacatctc
    36001 catgaaggat cacttctgct ccaagatctt ttgttctgtt aacctttaac agtggagtgg
    36061 ttgttggcat aacgatcgtt gctttgactc cctgtcttgc tgctgccaat gcaacaccct
    36121 gtgcatggtt tccagcagat gctgtgatca atccttttgc tttttcttct tcagataatg
    36181 tactgatctt gtaatatgca ccacggatct tgtaagctcc agtcaactgc atgttctcag
    36241 gttttagata aacatcgttt cctgtctgat ctgaaaaata atcacttttg atcagcttgg
    36301 taggaaggac aacctcctgc actttattat atgcagcttc aaaatctttt agttctaaca
    36361 ttttaatttt cttctccttt ttcgaataat gcattgacaa acatatctgg atcaaatctc
    36421 tgaagatcat caacctgttc cccaatacca atgtatttta ctggaattcc aagttctgac
    36481 tggattgcga tcgcgattcc gccttttgct gttccatcta atttcgtcaa aataattcct
    36541 gaaatgtctg ttgcttcttt aaactgtttt gcctgtgcca tggcattttg tccagttgtt
    36601 ccatctaaga cgatcagtgt ctctaaataa gcttcactgt attctttttg gatgatacga
    36661 ttaatcttgc ttaactcatt catcagattc ttcttattat gaagtcgtcc tgctgtatca
    36721 cataacaaca catcgacatc tcttgctttt gctgctgcta ccgcatcata tacaacagcc
    36781 gcaggatctg caccctctgc ttgactgatg atatcaaccc cagcacgatt tgaccattct
    36841 ttgagctgtt cgatcgctcc agcacggaat gtatccgcag ctgccagtaa tacttttttt
    36901 ccttgtcctt ttagtgatgc tgcaagtttt ccaacagatg ttgttttccc tacaccattt
    36961 actccaatga caagaacgac actcttttta tcaataaaat cataagcact atcatccagt
    37021 ctcatctgat ccttaataga atcgatcaaa agctgtcggc attcttccgg ctctttgatc
    37081 ttattttctt ttactttttt cttcagatct tcaatgatat tcattgttgt atcaattccc
    37141 atatcactca tgatcaaaat ctcttccagc tcttcataaa aatcctcatc tattccagaa
    37201 aatccgctga aaatagagtc aaaacttgag gcaatattat ctcttgtttt acttaatccc
    37261 tgtgcaagtc ttttaaaaaa tccttttttc tctgacatag ccattctcct tttattcatc
    37321 taaatcattc tcgatcaaat ttacagaaac ctgagttgaa ataccttttt cctgcatcgt
    37381 gatcccatat aaaacatctg ctgccatcat cgttcctctt cgatgtgtga tcacgataaa
    37441 ctgtgtatcc tttgtaagtt tatgcagata ctttgcaaat cttgtgacat ttgaatcatc
    37501 caatgccgct tcaatctcat ccaggagaca aaacggagat ggttttaaat tctggatcgc
    37561 aaataataat gaaatcgctg ttaaagcttt ctctccacct gataactgca tcatattctg
    37621 aagtttcttt cctggaggct gtgcaatgat cctgattcca gattctaata catcatcatc
    37681 tgtaagttcc aatcttgctt ctccaccacc aaataattcc acgaatactt cctgaaatac
    37741 tcgtttgatg tctttaaact ttgcattaaa ctgacggcgc atcgcatctt ccagttcttc
    37801 catcaaacct gtcagatgtg cttctgctgt cacgatatct tcatgctgtt tctgcataaa
    37861 ttcgtaacgt tcggaaactt ctttataatc ttcgactgca ttgatattaa ctggtccaag
    37921 gttcttgatc ttatttttta actcagagat cgtttcctta acctttgtaa ggctctgcgt
    37981 ctcatccaga tcgatcctct gttttgcctg atgatatgtg atctcgtaat tctcccacat
    38041 ataatccaaa agctcttttt tctttccttt gcgattctct ctctgactct ccagacgata
    38101 catttctttg tcatatccac tgatacgttc aagcatctct tcgcgttctt taaggatatc
    38161 cttataaagg acttctttct ccttcacttc ttcctgcatc ttctgatgcc tgttctgatc
    38221 ttctatgatc tttcgttcca ttgcttcgca tgattctttt aaatgtccaa tctgctgttt
    38281 taatgctttt acattctcat tgatacttcc agtatctttc tgattttctt gaacctcttc
    38341 tgatagatgt tcgatctcag atcttactcg ctcctgatta ttctcctgaa attctaactt
    38401 ctgtgattct ttgcttaact gcatatgaac cttggaaact tccattgcat gctgttcggc
    38461 atgttttcta agatgttcaa gaatctcact gatctccttg atcctttctt catcctgctc
    38521 atttgcactt tcaagatcta cctgttcatc ttgcagtctg tcagcatcat cttccgcatt
    38581 ccttaattct tcttccagtt gttgtctctg tatacttaac tgtttgtgcc tattatcaag
    38641 ctcttctttt ctnnnnnnnn nnattgtgac atctgctgtg tatttaattc caaagagatt
    38701 tcttgcatct ctttctctaa gtctatcata ttttgctgat attctttgag ttttttttca
    38761 ttttcatgat atttttcttc atcttctgtg attgctttcg tccatcgatc cagcttcttc
    38821 tttaattctt caacttctct ggatcttcca agcagattac tcttattctt aaaagcacct
    38881 ccagacattg caccgcctcg gtttaaggaa tccccatcaa gcgttacgat ccttacacta
    38941 tgatgatatt tatttgcaat tttaattcct gcatccatat cttttacaac aagaatttgt
    39001 ccaagcagtg atgcaaataa atccttatat acttcatcat agatcacaag ttgattggct
    39061 gccccgatca cccctggttc taaaagaatt tctttccttg caaattcttt tcccttcact
    39121 gcatccagcg gcaaaaacgt aactcttcca taacgattct tcttcagata ttcgatcatc
    39181 ttcttcgctg ttttctgcgt atccgttaca atgttctgaa tacttccacc cagagctgtc
    39241 tcgatcgctg tttcgtattt ttcttccact ttaatgatat ccgcaacagc tccaatgatc
    39301 ccagatgtca cggcctgttg ttccatcact ttcttgatcc cgaatccata tccatcataa
    39361 cgttctgcca tattccgcat tgtttcatag ttggaacgca ctctatgata tttctctctt
    39421 ttctgatcca tctgttcctg aatctgacgg cgttccgcta tcaatgtttc ttcgattctc
    39481 ttggcattat gacattcgat ctcagatcgt tttttcttct cattgatctc ttcgatcttc
    39541 tgactagaag aatctttttc tttttcctga tcattgatct gccgttttat ttcttctatc
    39601 tgatgatcca gtcctatgat ccggacattt agctgttctt tcacagtctg ggaacgctca
    39661 atcgctccag ccacccgcat acggccttct ctgacagcat tcactcggat ttcaatctct
    39721 tcttgttctc tctccagatc ctgaatctgc ttctgataca atgcaacttc gctttctttt
    39781 tgtatctttt gttccatgac agattcatat tccttacgaa tttcttctgt ctctgcctca
    39841 aatctcttta actgtgcttt cttttcattc agatctttat acagcttatc cttagactcc
    39901 agatacatac tctcacgcat atgttctgta ttgatctgtt catttaaaac ctgaatcttt
    39961 ccttcttttt tctgctttgt aagcttcatt tcagaaatat gagcagtgat cgtttcgatt
    40021 tcctgtttta aatgatccaa aaactcttct tgttttccgt atttctcttt tgctttggca
    40081 agtgtctgat ttgcctcatt gatctcccgc tgagcgattt ctatcttttc atccagctct
    40141 tttaattcct tctcaagatt ctgattctca agcaggaaca acttcacatc ttcatctttc
    40201 aactgatctc ttaacaacag atattctctc gcacgttctg actgtttctt aagcggtcca
    40261 acctgacgtt ccaattccgt taagatatct gtgactcgta ccagattctc acgttcgatt
    40321 tccaaagatt tttgtgtttc cagcttgttt ttcttgtatt taacaattcc tgctgcttca
    40381 tcaaataatt ctcttctctc ttccggtttt ccacttaaga tctgttcgat ctgtccctgt
    40441 ccgatgatcg aatatccctc ttttccgatt cctgtatcaa aaaaaagttc tacgatatcc
    40501 tttcttcggc aggcacttcc gttgatcaaa tattcacttt ccccagaacg atagactctt
    40561 ctggcaactg ttacttcttc aaactggatt ggcagactat ggtcactgtt atctaatgtg
    40621 atcgcaacat aagcaaatcc catcggcttt cgaagctctg tccccgaaaa aataacatct
    40681 tccattctag aaccacgaag ctgttttgct ctttgttctc caagcaccca tcgaacagca
    40741 tctgcaacgt tactctttcc acttccattt ggacctacga ttcctgtgat cccatgttca
    40801 aatttaaaga tcattttatg ggcaaaagat ttaaagccat tgacctcaat actttttaaa
    40861 tacatgtcca cctctgtttc gtgttatttt ttgtggcgtt tgatcgcctc aaatgcagca
    40921 tgctgttctg ctgacttttt actatgtcct gatcctgttc cgatcacatt ctctccaaga
    40981 cgtgcttcta ccgtaaagat tttggcgtga tctggacctt cttctccaac aagcacatat
    41041 tttaatcgtc cttttccttc tccctgaaca atctcctgaa gattcgtctt tgcatcgaaa
    41101 aacagaactt tattttctac atctttaaat aaatatgtct caatgaattt tgttgctgtt
    41161 tcgattccct gatctaaata aatcgcaccg atcacagctt caaatgcatc agaaagaatg
    41221 gaatctcttg atcttccacc tgtcatatct tctcctttgc ttaagagaac atatttccca
    41281 aggttgatat cccttgcaca tcctgcaagt gtcggctcac atacaagact cgccctgatc
    41341 ttgcttaact ttccctctga ttctttctgg tatgtgcgat acagataatt acttgttacc
    41401 agctcaagaa ctgcatctcc caaaaattcg agacgttcat tattcttatc tttgctcatt
    41461 cttcgttcat ttgcatagga actatgaatg aaggcattcc aaagtcggga tggctcatga
    41521 aatttaattc ctatctgctc ttgaaattct tccacacttt ttgcatccat tttcttctta
    41581 tcctttcaca atctggtctt tgatcttctg cgtaatatct tcttcggcaa atgcgataca
    41641 ctgaatgatc gcatttttca tttcttttgc acttgaagat ccatgtgctt ttacaaccaa
    41701 tccatttaat ccaagtaatg gcgctccacc atattcagaa gcatcaaatt tctttaacgt
    41761 cttctttaat gctggtttcg ctaatagtgc accgatctta ctctttaatg ttgacatcat
    41821 tccttctttg atctgactga ctaacactgc tgctaatcct tcttccagtt tcagaacgat
    41881 atttcctgca aatgcatcac atacaacaac atccgctgca ccgtatggga tttctcttgc
    41941 ttcgatactt ccgatataat tgatattttc acattccttt aataatggac ctgtttcttt
    42001 taccaactga tttcctttgg cttcttccac accgacattg acaagtccga ctcttgggtt
    42061 cttgattcca agaacatctt ccatatagat agatcccatc tgtgcaaact gaagcagata
    42121 ttccgctttc gcatccatat tcgctccagc atcgactaat aacatcggac ctttggctgt
    42181 tggcatcact gctcccagtg gtgctctctt gatccctttg atccttccaa caacaaactg
    42241 tcctccaaca aggatcgctc ctgaacttcc agctgataca aatgcatctg cagtcttatc
    42301 ctttaccatt ttcagggcga ccaccatgga agaatctttc ttcttacgaa tcgcttctac
    42361 tggtgattcg tcacaagaaa tttcttcttc tgcattcaca acttcaattt gttcattttt
    42421 atactgatat ttgcttaatt cttcatgaat ccttggtgct tttcctacaa gaaatacttt
    42481 cagattgttt ttttcattca cagcatcaac ggcacctttt acaacttctc ctggtgcatg
    42541 atctccaccc atggcatcaa ctgccacttt cactaatttc tccatatgac ctgttactcc
    42601 ttttcttaaa aataggaaat gccacacgcc taaaccttct taaaaagcct aaacgtatgg
    42661 catttttctt catttaccat tattcagcgt gtgctgaaac aatttccttt ttattgtaag
    42721 atccgcatgc tttacatact ctgtgaggca tcataagagc accacatttg ctgcatttt
//
LOCUS       DAOMUE010000002        29871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_188_length_29871_cov_3.230447, whole genome shotgun sequence.
ACCESSION   DAOMUE010000002 DAOMUE010000000
VERSION     DAOMUE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 29871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29871
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188_length_29871_cov_3.230447"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(394..1941)
                     /gene="guaA"
                     /locus_tag="PLC71_00200"
     CDS             complement(394..1941)
                     /gene="guaA"
                     /locus_tag="PLC71_00200"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008374035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRM01099.1"
                     /translation="MKNELVIVIDFGGQYNQLVARRVRECNVYCEIYSYKIGIDKIKE
                     MNPKGIILTGGPNSCYEPDSPTYSKELFELGIPVLGLCYGAQLMQHVLGGKVEKAEVS
                     EYGKTEVLIDKKDSKLFENVSEKTICWMSHTDYISQVAPGFEISAHTADCPVATAENA
                     DKSLYAIQFHPEVLHTVEGKTMLSNFVLGVCGCAGDWKMDAFVESTIKEIREKVGDGK
                     VLLALSGGVDSSVAAGLLSRAIGKQLTCVFVDHGLLRKDEGDEVEGVFGENGQFDLNF
                     IRVNAQQRYYDKLKGVTEPEAKRKIIGEEFIRIFEEEAKKIGAVDFLAQGTIYPDVVE
                     SGLGGESAVIKSHHNVGGLPDFVDFKEIIEPLRDLFKDEVRQAGLQLGIPERLVFRQP
                     FPGPGLGIRIIGEVTEEKVKIVQDADAIYREEIAKAGLDREINQYFAALTNMRSVGVM
                     GDFRTYDYAVALRAVKTIDFMTAEASEIPYEVLNKVMNRIINEVKGVNRVMYDLTSKP
                     PGTIEFE"
     gene            complement(1980..>2593)
                     /gene="metK"
                     /locus_tag="PLC71_00205"
     CDS             complement(1980..>2593)
                     /gene="metK"
                     /locus_tag="PLC71_00205"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRM01100.1"
                     /translation="RLDAVVLSTQHDPDITQEQIHEDIKREVFDKILPADMVDDETKF
                     FINPTGRFVIGGPHGDAGLTGRKIIVDTYGGYARHGGGAFSGKDCTKVDRSAAYAARY
                     VAKNIVAAGLADKCEIQLSYAIGVAEPTSIMVDTFGTGKKSNQELVDLIRKHFDLRPA
                     GIIKMLDLRRPIYKQTAAYGHFGRNDLDLPWERLDKVDLLKGE"
     assembly_gap    2594..2613
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2614..3181)
                     /locus_tag="PLC71_00210"
     CDS             complement(<2614..3181)
                     /locus_tag="PLC71_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HRM01101.1"
                     /translation="MDKVLFTSESVTEGHPDKICDQISDAILDALLEQDPMSRVACET
                     CTTTGIVMVMGEISTKAYVDIQKIVRDTVREIGYTRGKYGFDADTCGVITTIDEQSSD
                     IAMGVDKALEAKENNMTDEEIDAIGAGDQGMMFGFATNETEEYMPYPISLAHKLSRQL
                     TKVRKDGTLSYLRPDGKTQVTVEYDDGKP"
     gene            complement(3258..3896)
                     /locus_tag="PLC71_00215"
     CDS             complement(3258..3896)
                     /locus_tag="PLC71_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HRM01102.1"
                     /translation="MRQYLKNEVFKVSKWVELFISAIIMIAVIGGTIYLVKDVVQLMM
                     NYPTSRDIYKFVGVAFNLVICIEFVKMLCKHTPDTLVEVMMFAIARQMIVEHTSPQQN
                     LIVVLAIAILFAIKKFFFGKFDDIDKTVFLPTQRISSINELVHVEIPKETQHQTLGEL
                     LEKQIGTESGNFQVGQCYFFKGGALRVAKVKNGKITQIEVIRSKNAPVVLKR"
     gene            complement(3913..5208)
                     /locus_tag="PLC71_00220"
     CDS             complement(3913..5208)
                     /locus_tag="PLC71_00220"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRM01103.1"
                     /translation="MEQYIIKGGNPLVGEVVIGGAKNAALPVLAAAVMTDGKCMIDNM
                     PDVRDINVLLQAMQEIGADVDRTGKHEVTISGKGIHPECDVDNEFIRKIRASYYLIGA
                     LLGKYKRARVALPGGCEIGSRPIDQHIKGFKMLGAQIEIENGMISATAEELKGAHIYM
                     DVVSVGATINVMMAASLAKGNTIIENAAKEPHVVDVANFLNSMGAKIRGAGTDVIRIK
                     GVEKFGDCQYSIIPDQIEAGTFMTAAVATKGDIMIKNVIPKHLEAISAKLIEIGAEVV
                     EFDDAVRVSATKRLESTNIKTLPYPGFPTDMQPQMAVTLALSNGTSVISESIFENRFR
                     YVDELTKMGATIQVDGRTAIISGVEGFTGADVHAPDLRAGAALVIAGLSAKGFTTVSD
                     IGYIYRGYEQFEQKLKQLGGEIQLVNNEKEVTKFKLKIG"
     gene            complement(5307..5810)
                     /locus_tag="PLC71_00225"
     CDS             complement(5307..5810)
                     /locus_tag="PLC71_00225"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRM01104.1"
                     /translation="MAEKNNNYILIGMPGSGKSTIGVLLAKALGYDFVDTDLVIQQKG
                     GGLLKEIIARIGNDGFKQLEEDVNASIDVNKSVIAPGGSAVYSDKAMRHFKEIGTIIY
                     LKLDYQTVKRRLGNLKARGVVLEKGQTLRDLYNERIPYYEKYADITVKLDKLSIGNSL
                     EKVLENL"
     gene            complement(5794..6387)
                     /locus_tag="PLC71_00230"
     CDS             complement(5794..6387)
                     /locus_tag="PLC71_00230"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRM01105.1"
                     /translation="MAKTSWKPGNMIYPLPAVMVTCRDKGQDNIITIAWTGTICTNPP
                     MTYISVRPERHSYEMIKNSGEFVINLTTKQLTRATDFCGVRSGRDVDKFKEARLTKEE
                     AQVVDAPIIAESPVNIECKVEKIVPLGSHHMFMAKVVNIMVDDDYMEDNGRFALEKTN
                     PLIYSHGGYYETGKKLGTFGYSVRKKKKRKKPNGRKK"
     gene            complement(6397..6813)
                     /locus_tag="PLC71_00235"
     CDS             complement(6397..6813)
                     /locus_tag="PLC71_00235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRM01106.1"
                     /translation="MKKEDCIFCKIAGGEIPSNTIYENSEFKVIMDIAPASKGHVLII
                     PKEHFKDIYEIDAITAGKLFQLATVVARALKEVLHCDGLNVLQNNGLIAGQTVYHFHM
                     HLIPRYEGDDVTIGWKEHSTEDIDMEQLRQDIKKAL"
     gene            complement(7218..7814)
                     /gene="recR"
                     /locus_tag="PLC71_00240"
     CDS             complement(7218..7814)
                     /gene="recR"
                     /locus_tag="PLC71_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRM01107.1"
                     /translation="MDYYSNQITQLIEELGKLPGIGPKSAGRLAFHIINMPQDQVQKL
                     SDTIISAKTNVHYCKECCTLTDNEICPICASDKRDHSTIMVVENTRDLMAYEKTGKYE
                     GVYHVLHGAISPMLGIGPDDIRLKELMARLQGDVKELIIATNSSLEGETTAMYISKLV
                     KPTGIKVSKIASGVPVGGDLEYIDEVTLLRALEGRTEI"
     gene            complement(7830..8183)
                     /locus_tag="PLC71_00245"
     CDS             complement(7830..8183)
                     /locus_tag="PLC71_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRM01108.1"
                     /translation="MAKRSGGFPGMGGNMNQLMKQAQKMQKQMEETTKALEESTYEAT
                     AGGGVVKVVVSGKKEVVSVHLEEEVVDPDDVEMLEDLIVAATNEALRKMEEDSQEKMG
                     KVTGGLGGMGGGFPF"
     gene            complement(8224..9828)
                     /gene="dnaX"
                     /locus_tag="PLC71_00250"
     CDS             complement(8224..9828)
                     /gene="dnaX"
                     /locus_tag="PLC71_00250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRM01109.1"
                     /translation="MSYMALYRKFRPTRFSEVKGQDHIVKTLENQMRTGRIGHAYLFC
                     GTRGTGKTTVAKILAKAVNCEHPVDGEPCGECEVCKAIAEGSSMNVIEIDAASNNGVD
                     NIREIKEQVQYSPATGKYKVYIIDEVHMLSIGAFNALLKTLEEPPEYVIFILATTEVH
                     KIPITILSRCQRYDFHRITAATIKKQLSDLMEQEHVDTEDKALEYVARMADGSMRDAL
                     SLLDQCIAFYLGEKLTYDNVLEVLGTVDIQVYSDLLDMILSQDINGVMDLLEDVARQG
                     REWSQFITDFLWYLRNLLLVGQGNAAEEALEVSTDQLKLLQEKAQQVEENTLIRYIRI
                     LSELLERIRYATSKRVLVEVEMMKMCRPQMEADTNSLADRVRQLEQQLEQLLQSGIPV
                     QQAAQAGQNQIALQQEVKSEQDPVEQLNEKFPPAKVEELIELVNHWRDVKRNLSPQMK
                     YCLQRATLSVNEEGTALRVLFPKEMNVESGYFLEEPERIEQLKQELSRQSGRDVELEV
                     SVMNTDNDDSDMINLDCIHMDVKIED"
     gene            10021..10740
                     /locus_tag="PLC71_00255"
     CDS             10021..10740
                     /locus_tag="PLC71_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM01110.1"
                     /translation="MNDLQELSTFSHEINNSLTLIYGQIQCIEKTYDTLTGNDHWNRM
                     KDDFSSLFDFIHQFLAPAEESSAVMEPLDLISVISEIRSSWSYYLHKEQIRFFIQADP
                     GTAFYVYGARSKLFQLFHNLISNAVKAIQQKKETCRSPHITHITVHLSKEQGHIVLNL
                     SDTGIGMTTEQQSRAFYRGVSFHPGGNGIGLSVVQNIARDLHIKIHIDSAPNQGTTFT
                     LIFPAYEQQLNCPTRTEALTK"
     gene            complement(10670..11653)
                     /locus_tag="PLC71_00260"
     CDS             complement(10670..11653)
                     /locus_tag="PLC71_00260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRM01111.1"
                     /translation="MYQKIIELLRIHGYMKNDTETGPCYVKNINGRNKCVFVIFDKNP
                     DGSSILEEDLYHLEDYARQLFGMDCVVLMLILSQNDKPVFLDPKDQFDDLYGPLSIVL
                     AQNEVSQAGQGSEIEVKDNKSVKDFWKSLYQYKATLLIFTANLICFILTETYGDKIYD
                     SFACNAYMVKKLHEYYRLFTSNYLHFGWDHFFNNMAVFLILGSSLEKVIGSVRYVILY
                     TGAGIAGSIISVAYYSMIGQDVLSAGASGAIFGLVGALAAIFLFCKEQRQRFDGFGIF
                     LMIAGSLYHGFQSGTTDNAAHIGGCIAGFILSMLLYVLGSLIAAHKQGRSV"
     gene            complement(11700..12986)
                     /locus_tag="PLC71_00265"
     CDS             complement(11700..12986)
                     /locus_tag="PLC71_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HRM01112.1"
                     /translation="MHLKEQWIREGFSSYYVVKKEQKLTYEKNILYNHTLPCLLPCEF
                     RIEDGEEYYYYETGIYTKLKDRISMLEPKLFFAYLLEVFEQVASYLLELDHLKLDLES
                     MFLDKENRPVLCYLPEYEKKIDEQLRDFIEECIEYISGDDKKRVRFYYEFHSFLIKEK
                     PNMEQMKDYLEVRSEKMNGEILREKIKNEESKSAQNMQENLSDLIRDEKDSPEKDEIK
                     IYEKTPKRILILIGKIISVVGCFCSILGIVYFVLKIFEYGFYYLFLIGSLVSIAGLGV
                     SVYGCYKLWKKNKNPKTFVDSWQKTENAAELLDEKTILLEDEDDRTVLLLEEPIGRLV
                     PKNPEQKEIVLDDNGFVIGSSAQGTDHQLEGTGISRRHIRFYKEDGNVCCEDLDSTNG
                     IKINGVKLNGKKNKKAVLKEGDCIKIGLEEFYFKQD"
     gene            complement(12961..13455)
                     /locus_tag="PLC71_00270"
     CDS             complement(12961..13455)
                     /locus_tag="PLC71_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01113.1"
                     /translation="MTVEISALLPIILMVLWMFFSYLFYFMNCGIAQGIMEEAVQKAA
                     DVKITEAEYGTGKLSYQKVNQKLITGNVISSNKSGNTKAEKEMKEQLRRHLFMAEVSS
                     VRVSTTPLRVSAKIKTRSAVVAGDFLSMFGICLFEYEGNDQAQGDFEIDQIRRWNALE
                     GAMD"
     gene            complement(13452..14339)
                     /locus_tag="PLC71_00275"
     CDS             complement(13452..14339)
                     /locus_tag="PLC71_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRM01114.1"
                     /translation="MFSCQKHFSKVNPVQNDNNNNKIVVKKSKRASSSASDLSGNEEG
                     AMTLEASLIVPIFLIVLLMLTSAGEILMIHQQISHGACEAAKRAAVNEYRIRQKKKSG
                     SDLSGFSAKAAFLAAVNRKFLDHSALIGGSAGAAAACRLTLTSKGEYIVSVRYYIRKT
                     MPFLSTHTVSYEQSVRQKSMTGYVPDGDELKKGYVYITPHEAVYHKDLSCTHLSLDIS
                     VDEDVEKYRNKRTQYKPCDKCTKHEKGDFSCVYIAKEGESYHTDLGCSGLKRTVKQVD
                     LSTLKGMRPCTRCAKQGDT"
     gene            complement(14380..15798)
                     /locus_tag="PLC71_00280"
     CDS             complement(14380..15798)
                     /locus_tag="PLC71_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HRM01115.1"
                     /translation="MSAFLAMLFLVFLLLISVCVEGIYMYTARGKAMGIYMSGLSHTK
                     GNYQKELADMYHIYAMDPRYHKKIETDFSDRMKESLDQNGDPFRFQTGSTKISDILDL
                     SAQKGEVLKYQIRQQMQYEAAGDILKNLTKKIKAGEDQKNELSGIKDQIQKEEEEAEE
                     TENEKTLTSEPVKKDPRKGFMKLLKEGSVPLIMGEKKVSDLPINIVYGKKDTTKQSAW
                     NFMNRKDVEKELDEFEKTASTDSFASELPVVLYATKYFHFLTDTTKKDGIKYEIEYLI
                     AGKDSEKENLGMVFWRMIALRFVMNAACVYQDPAKEKEAALLAASILGVTGFPPAVAV
                     AKNLLLLALAYGESVIDVRNLADGKKIPAVKTSSDWQLSFAGLATLNCKRKPVKQGIS
                     YEDYLLLLLISQKDKRQKYFRMMDLMEQNIRRKVSDFKLDQCISSYKITQNLKLKKLG
                     FGGMILPFATYTDLKMYRYVTY"
     gene            complement(15819..15992)
                     /locus_tag="PLC71_00285"
     CDS             complement(15819..15992)
                     /locus_tag="PLC71_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HRM01116.1"
                     /translation="MRNFLKHFFKDESGMGVVEVILIIVVLIGLVIIFQTQIKKVVDS
                     IFKTITSKTGQVK"
     gene            complement(16013..17350)
                     /locus_tag="PLC71_00290"
     CDS             complement(16013..17350)
                     /locus_tag="PLC71_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRM01117.1"
                     /translation="MKFKFQWINKIVEKLPEELKEKRKAEIGSCFIGTKKQLDEKVRK
                     SLYEQVKIISVATIIFLIVLFLTIIFYFSQDRRILITRDETGGEVTEQQIRIKTKSDD
                     DIYTLEIHPKGYTKAQITQAFDRGEAYLRKHLVAKNKSLNEVERNLEMPVKIPGENIA
                     VQWQSDDPTVIDEEGNVFSKNVKKDYLLNLDARMECQGEVRILKIPVCVVPGNSGKKV
                     SEKQKIIADLKKLEEKHITKEQFYLPDVIRQGRLLPAENESQIPAILFLGIAVIIFFW
                     YHENEKYKEQRIQTLEQSMVEYPQIIEHLILFLGTGMSVVAALEAVATEYEKEQRRGN
                     KKEIFVYEQIKKTCRQISFGVPQTKAFGEMGKKIGLSSYQKLSVLLVQSITRGSTDLF
                     LRLKEEEEGAFFEKKEQAKRKGEQASTKLLAPMMVMLVVILVLLMFPALSTFS"
     gene            complement(17340..18098)
                     /locus_tag="PLC71_00295"
     CDS             complement(17340..18098)
                     /locus_tag="PLC71_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRM01118.1"
                     /translation="MKHIRNLWNLLKQEISLWKYEKQELVKGICYIVMIAWLFFDRIA
                     FAILLIPYLFVFMKQQRIHKVHREKEKLRKEFREMMISIGNSLSAGYSIENALKTAKN
                     DLEMYEEHSLLAKELQLLINKLKMNEPVDNLLFDMAEHVGLEEFYQFAQVISIAKKSG
                     GNLIEITENTIEHLSQAIQTKEEIHTMIAAKQMEKKIMSVMPYFILLYVRIANPGYFD
                     ILYESFAGVLVAVISLLCLWIADIWAERVTEIEI"
     gene            complement(18037..19275)
                     /locus_tag="PLC71_00300"
     CDS             complement(18037..19275)
                     /locus_tag="PLC71_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HRM01119.1"
                     /translation="MALKELLKQRVMEKLNFSQEISDEHLKGVIQEEIMKISEEYPLL
                     LSDKIRLNQEVFYALRRLDILQDLLEDDSVTEIMINGYKNIFIERQGKLHRYPGHFSD
                     NEKLYQVIQQVASGANRMVNERNPIVDARLNDGSRVNIILPPISIDGATMTIRKFAKE
                     PMTLAWLCEREAFSEEIAKFLKILVRARYNIFISGGTGSGKTTLLNGMSNCIPKDERI
                     ITIEDSAELKLNGIDNLVRLEMRNANAAGENQVDMKELIKAALRSRPDRIIVGEVRGE
                     EALSMLNAMNTGHDGSISTGHANSCKDMLKRIETMVLMGVDMPVEAIRGQMASAIDVI
                     IHLGRSFDGSRKLMEISEITGMAASQVALHPLFEMNEDDELTMRSELCDQKKLKEYGQ
                     YEAYQKLMESFKARDQPVEI"
     gene            complement(19266..20114)
                     /locus_tag="PLC71_00305"
     CDS             complement(19266..20114)
                     /locus_tag="PLC71_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01120.1"
                     /translation="MKDISIGIYHKDHQYGKRLMEYLNHQKDFPMTASFTSDEEMFFK
                     QEQEGDFECLVLAEETDYHGDFPVCRIGANESMGGMYCQSAKEIAAGIYHCLNVSPQI
                     ENERIFGVYSPISRLEISAFARNMAASNGWIYFGMQPYGHFEEDETDDLLLFYIKEHK
                     EDIVLYFMEHQKKFDGCVGFAGASCYLDYRELSMQDYEWFFEKLRETGICIIFDIGIA
                     SPPDLKFFGLFDRIFLPLMRQDLGCMEYKKFQKQMRKHGVWKLTNWEEVMLESWKNKG
                     GRGQWP"
     gene            complement(20111..20614)
                     /locus_tag="PLC71_00310"
     CDS             complement(20111..20614)
                     /locus_tag="PLC71_00310"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HRM01121.1"
                     /translation="MKIWILLFLIMAAIYDYRDFKIPNKLNGAGALITLVLARVSGMK
                     AADMMIGVLVVLISCGLLFYIGCLGGGDVKLLMVCAISIGRAMPRFLILSFICNGIYA
                     VIFLWKRKNFHLRLFRLFQYVTQCKDNKRLLMYETENGDLMKDGMIHFSFGILAAYMI
                     CLLGGGL"
     gene            complement(20738..21010)
                     /locus_tag="PLC71_00315"
     CDS             complement(20738..21010)
                     /locus_tag="PLC71_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HRM01122.1"
                     /translation="MGQNEMIFYIVAAGCMICLMIGIVKKPAYLLVLLLRGAFCAMIV
                     YGISIVCMRKGITAPVNLNILSIGTGALLGLPGIIVLYAAAVFLLQ"
     gene            complement(21083..21943)
                     /locus_tag="PLC71_00320"
     CDS             complement(21083..21943)
                     /locus_tag="PLC71_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HRM01123.1"
                     /translation="MRKFIGKFLAAGLCVAMALSSVTVKADSVDQKYLALGADLTASQ
                     KKKVLELLDLDDNSDYKVITVTNKDEHEYLDSYLSSRVIGTRALSSVTVEQKDDGNGV
                     NVTTQNISYCTSGMYRNALITAGIKNADVKVAGPFKISGTAALVGVMKAYEEMTGKKI
                     PEKSKDAATDELITTGEVAENIGSDDAEKLIADVKQKVAKDNLSSPSEIKQAIEESAK
                     DLNINLSDADRAKIQSLMDKISGLDLNVSQLKSQAKDLYDKLGGSQGIFDKIAAFFQS
                     IFSWLSNLFS"
     gene            complement(22038..>23856)
                     /locus_tag="PLC71_00325"
     CDS             complement(22038..>23856)
                     /locus_tag="PLC71_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRM01124.1"
                     /translation="QTSSAKLQFPKELWYGTFGNLFSRHFLGTTPLTMAVDDSKINLY
                     CGILTLLMAGFYLAVREIRLIDKIRRLLLLVFLFFSFNMPVLGYVWHGFHDQYGIPNR
                     FAFLYIFALLAMAYEGYCVLVRGKRKVSLWIYLSVILCGILIGITMKTSTMKLEMKTV
                     YATVAAIAVYMICFALYQKKIFRKKIFTTLICFLFIVETAAMAVMGFQKNGTVDTDSY
                     FQDTKAITEVKKEYQKNVETRMELISGRMLDESIWHTLNGMTLFGSTAQGNVVDMMDQ
                     LGFYTGVNEYLYEGATPFTNNLMSMKYQIYRPYDTKYTEFSLKESVGNVTVYKNPYRT
                     ALAYTMDDLVQTWDYEDYNPFYVQNDLATSAFDVDELFHMVKTAKPQLNDCKITSDNG
                     DGEYVFENTSARPDNMVFTIRSTKTRRLYIHFDGSQVENTVIEKNGEQVLTGRLDSQI
                     IYLGNVQKGDEIRIKMQLKQDNEMSGVVRLTAAELDEEVMEELAQRMQENAWKLTSAK
                     GNHLSGTIHAQEDQMLFFSIPYDKGWTVKIDGKKVKTKALGKAFLTVKVPEGKHKVSL
                     TYVSSGFKEGAILSVVGFVIFILITAFFRRKKKAAVKEI"
     assembly_gap    23857..23886
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23887..24618)
                     /locus_tag="PLC71_00330"
     CDS             complement(<23887..24618)
                     /locus_tag="PLC71_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRM01125.1"
                     /translation="MTNKIKDILKTHKYLLLSFFLPVLLLEGIAIAEKIQPFGSESFL
                     IVDALHQYLPFFADYQEKLKSMDSMFYSFHAGLGYNFLGLWAYYLASPLNLVIALVSK
                     SMLTMILSHLYVLKIALCCFTAAFYFRKRRGKDEISIVAFGMAYGLCSYMVGYSWNIM
                     WMEVMMMLPLILYGIDKLIKEHDGRLYCFALFISLWCNFYMSYMTCLFLILWYLLYSH
                     KNVKEFFTNGFRFAGYSLLSGAMAAV"
     gene            complement(24581..25327)
                     /locus_tag="PLC71_00335"
     CDS             complement(24581..25327)
                     /locus_tag="PLC71_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM01126.1"
                     /translation="MKSVAMAFYNGAKYIDEQIRSILDNMEETDELIISVDDASDGSE
                     AILEDWVQNDQRIRIIKGPGKGVVKNFENAFKHCRGEIIFLSDQDDVWKKNKMSEIER
                     VFEDPKVMAVVHDAQIVDEKLSSLDQTTFEWRNSGTGFWKNMKKNSYIGCCMAVRRSA
                     MKRILPIPDDIWIHDQWIGLLSEQLGKVVFLEEPLIYYRRHGGNVTELTHGSITSMIK
                     KRYHMIMGINHRVKEWSRHDKQNQRHTENS"
     gene            25533..26867
                     /gene="gdhA"
                     /locus_tag="PLC71_00340"
     CDS             25533..26867
                     /gene="gdhA"
                     /locus_tag="PLC71_00340"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRM01127.1"
                     /translation="MSYVDEVIEQVVAQNPAEPEFHQAVKEVLESLRAVVEANEEAFR
                     RDALLERLVNPERQFKFRVPWVDDKGQVHVNTGYRVQFNSAIGPYKGGLRLHPSVNLG
                     IIKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDREIMAFCQSFMTELCKYIGADT
                     DVPAGDIGTGAREIGYMFGQSKRIRGLYEGVLTGKGLSYGGSLARTEATGYGLLYFTD
                     EMLKCNGKSIKGATIAVSGAGNVAIYAIEKAQQLGAKVVTASDSTGWIYDKDGIDVEL
                     LKEVKEVKRARLTEYAAARPSAEYHEGRGVWSIPVDIALPCATQNELHLEDAKQLVAN
                     GCYAVAEGANMPTTLEATEYLQENGILFAPGKASNAGGVATSALEMSQNSERLSWTFE
                     EVDGKLQNIMVNIFHNLDDAAKRYGMEGNYVAGANIAGFEKVVDAMTAQGIV"
     gene            complement(26978..28309)
                     /locus_tag="PLC71_00345"
     CDS             complement(26978..28309)
                     /locus_tag="PLC71_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM01128.1"
                     /translation="MKDLTKGKISTLILGFAIPVFLGNLLQLTYSLADTRIVGSFLGE
                     RSLAAVGATTTISNLIVGFLLGLANGFAIITAQKFGAGDKKRVRRSFAIALVLGVIVS
                     AILILIGTVFINPILGFLNVPGKLFIEAKQYILIIIIGLWVTMFYDILMAVMRAIGDT
                     LTPLLILAISVGLNIVLDLLFVAVWKTGTWGAAAATVLAQFIALVICCFYMIHKYELL
                     RLNKNDFKDLESKMIKEMLATGMSMGFMSSLVNIGSLTLQTAINKLGQEIIVAHTAAR
                     KITEIYMIMFSVFGQTMATFCGQNMGAGKVDRIKKGMKLAIIYTCVWSTFAMIASYTI
                     GPQLVHMVTGSHKDVVIVNATNYLKFNTIFYYVPAVISIVRNVMQGIGDQITPLVSSG
                     LEMVGKVVIAFTLVPLMGYAGVIVAEPIVWFIMVIPLLVQIIRTPKLKANK"
     gene            complement(28306..28533)
                     /locus_tag="PLC71_00350"
     CDS             complement(28306..28533)
                     /locus_tag="PLC71_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01129.1"
                     /translation="MDTYYVTRIEEPDFGCEGRPEGQEIKDKVYLKEEITKEETIIFM
                     EDKLLYERDINEGMKVVIDGDGRLQKAEDRS"
     gene            complement(28594..28911)
                     /gene="cas2"
                     /locus_tag="PLC71_00355"
     CDS             complement(28594..28911)
                     /gene="cas2"
                     /locus_tag="PLC71_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009220634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRM01130.1"
                     /translation="MDYFEFETLVEDEGNDKYLILIIYDISDNKHRLKISKLLEGYGT
                     RIQKSAFEAWLTKKHFEKLLSKLKEMTRVTDNIRIYKLHGYGEVTVLGDQNYVNGDDV
                     III"
     gene            complement(28914..29858)
                     /gene="cas1"
                     /locus_tag="PLC71_00360"
     CDS             complement(28914..29858)
                     /gene="cas1"
                     /locus_tag="PLC71_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009220635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HRM01131.1"
                     /translation="MVECQDGSERLVPKETLESIVIFGNVYLTVPCIKECLERGIAVS
                     MFSTKGKYFGRIESTNHVNAKRLKQQVYLSDQEEMRVQFSKKIILNKIHNQRVLLTRY
                     QRSTGKNIQENIQAIKIHENKISHCTNIEQIMGYEGNAARQYFAALSKMIKEDFKFSG
                     RNKRPPRDAFNSMISLGYTILMYEIMGEIENRGITPYIGFMHKDIERHPTLASDLLEE
                     WRAVIVDATVMSMIQGNEISINEFSKDEETGAVIISKNGVNLFIKKLEKKLQSNMNYL
                     EYLEHPVSFRRAIWWQVSKIVKCIDNGNFDDYMPIRIR"
BASE COUNT         8277 a         6834 c         4509 g        10201 t
ORIGIN      
        1 cttcatatac tctgtactgg cagatgcaaa atcggacatc attttccgaa atttatcaaa
       61 ttttcttgtt ttttaattct ccatcagtgt gattactgtg tcatggagtt taagctcatc
      121 gaaaataaag aacctttctg tggtatctgt tttctaaatg caggcaacaa aaaaacagtc
      181 tgccttgacg aaaatcactt caatcaaaac aaactgttct tcatatttac tctaatttta
      241 ttttatgaga ataactgatt tcacgaaatt tgcaggtgaa tctggaatat aaacaagaga
      301 aaaaaataat ttttaaactc catttgcact ccatttggtc ttttcgacac cccgcaaagc
      361 ctttatttat gggcttttcc gcgatttttc aagctactca aattcaatag ttccaggtgg
      421 tttagaagtc aagtcataca ttactctatt aactccctta acctcattaa taatcctgtt
      481 cattacctta ttcaacactt cataaggaat ctcagatgct tctgctgtca taaagtcgat
      541 tgtcttaaca gctcttaatg ctactgcata atcatatgtt ctaaaatctc ccattactcc
      601 aacacttcgc atattagtta atgctgcaaa atactggttg atttctctgt ctaatccagc
      661 tttggcgatt tcttctctgt agatcgcgtc tgcgtcctgg acgattttta ctttttcttc
      721 tgtgacttct ccgatgattc ggattcctag tcctggtcct gggaatggct ggcggaatac
      781 aagtctttct gggattccta actgtagtcc tgcctgtctt acttcgtctt tgaacaggtc
      841 acgtaatggt tcgatgattt ctttgaaatc tacgaagtct ggcaatcctc ctacgttgtg
      901 gtgggatttg atgactgctg attctccacc taatccgctt tctacgacgt ctggatagat
      961 cgttccctgt gctaagaagt ctactgcacc aatcttcttt gcttcttctt cgaagattct
     1021 gatgaattct tctccgatga tcttacgttt tgcttcaggt tctgtgactc cttttaattt
     1081 gtcatagtat ctctgctgtg cattgacacg gataaagttt agatcaaact gtccattttc
     1141 tccaaatacg ccttctacct cgtctccttc gtctttacgt aataatccgt ggtcgacgaa
     1201 tacacatgtt aactgttttc caatggctct tgataataat ccggctgcta cggatgaatc
     1261 tactcctcct gataaggcaa gtaatacttt tccatctccg actttctcgc ggatttcttt
     1321 aattgtgctt tctacgaaag catccatctt ccaatctcct gcacatccgc agactcctaa
     1381 tacgaagttg gaaagcattg tctttccttc gactgtatga agtacttctg gatggaactg
     1441 gatcgcatat aaggatttat ctgcattctc agctgttgca actggacaat ctgctgtatg
     1501 tgcagaaatt tcaaatcctg gagctacctg tgagatgtag tctgtatggc tcatccaaca
     1561 gattgttttt tctgatacat tttcaaataa tttggaatct ttcttgtcga tcagcacttc
     1621 tgttttacca tattcgctga cttcggcttt ctctactttt cctccaagga catgctgcat
     1681 cagctgtgct ccataacata atcctaaaac tgggattcca agttcgaata attccttgct
     1741 gtatgttggg gaatctggtt cgtaacaact gtttggtcca cctgttaaga tgattccctt
     1801 tggattcatt tctttgattt tatcgatacc gatcttgtaa gaataaatct cgcagtatac
     1861 attgcactcg cgaacgcggc gtgcaactaa ctggttatac tgaccaccaa aatcgatgac
     1921 gatcactaat tcatttttca ctcgatttcc tcctgagttg cctagtgatg tgtttcgttt
     1981 tattcacctt ttaacagatc aactttgtca agtctctccc aaggaagatc aaggtcattt
     2041 cttccgaaat gtccatatgc tgctgtctgt ttatagattg gtcttcttaa atctaacatc
     2101 ttgatgattc ctgctggacg cagatcaaaa tgtttacgga tcaggtctac caattcctga
     2161 ttggatttct ttcctgttcc aaatgtatca accatgatag atgttggctc tgctacacca
     2221 atcgcatagg ataactgaat ctcacattta tcagctaatc cggctgctac aatgttcttc
     2281 gctacataac gtgctgcgta agctgcagaa cggtctactt ttgtacagtc ctttccagag
     2341 aaagctccac caccatggcg tgcgtatcca ccgtatgtat ctacgatgat ctttcttcct
     2401 gttaaccctg catctccgtg tggtccaccg atcacgaatc ttcctgttgg gttaatgaag
     2461 aattttgttt catcatcaac catatctgct ggaaggatct tatcaaatac ttcacgtttg
     2521 atatcttcat gaatctgctc ctgagtgata tctggatcat gctgtgtaga taacacaact
     2581 gcatcaagac gacnnnnnnn nnnnnnnnnn nnnttggttt tccatcatca tattctacag
     2641 ttacctgtgt tttaccatct ggtcttaaat atgacaatgt tccatctttt ctcacttttg
     2701 ttaactgtct tgaaagctta tgtgcaagag agattgggta tggcatgtac tcttctgttt
     2761 cattagtagc aaatccaaac atcattccct gatctccggc tccaattgcg tcgatctctt
     2821 catctgtcat gttattctct tttgcttcta gtgctttgtc tacacccatg gcaatgtcac
     2881 tggactgttc atcgattgtt gtaataacac cgcatgtatc agcgtcaaat ccatatttac
     2941 ctcttgtata tccaatctca cgaacagtat cgcggacaat tttctgaata tctacatatg
     3001 cttttgtaga tatctctccc atgaccataa caattcctgt tgttgtgcaa gtctcacatg
     3061 caacacggct cattggatcc tgttctaaaa gtgcatctaa gattgcatca gaaatctgat
     3121 cacagatttt atctggatgt ccttctgtca cggactctga tgtgaataat actttatcca
     3181 ttctattatc ctttcttaaa atatatctgt tatttgtaaa atatcatgaa aagacccgaa
     3241 ggattccggg cctttattta cctctttaac acgaccggtg cattcttact ccggataact
     3301 tcgatctgtg ttatcttacc attctttacc ttggcaacac gaagtgctcc tcctttaaag
     3361 aaataacact gtcctacctg aaaattccca ctctctgttc caatttgttt ctcaagtaat
     3421 tctcccaatg tctgatgctg tgtttctttt ggaatttcca catgaaccag ttcattgata
     3481 cttgaaattc tctgagttgg caaaaatact gtcttatcta tatcatcaaa ctttccaaaa
     3541 aagaatttct tgatcgcaaa taaaatcgcg atcgctaaaa ctactataag attctgctgt
     3601 gggctcgtat gttccacgat catctgcctt gcaatggcaa acatcataac ttctactaac
     3661 gtatctgggg tatgtttaca aagcatcttt acaaactcga tacaaatgac gagattaaac
     3721 gctacaccaa caaatttata aatatctctg gatgttgggt aattcatcat taattgtacc
     3781 acatctttta ccagatagat tgttcctccg atgactgcga tcataatgat cgctgatata
     3841 aataattcta cccacttgga tactttgaac acttcgtttt tcaggtattg tctcattcag
     3901 gcaattctcc ttttatccta tctttaactt aaactttgta acttcttttt cattgttaac
     3961 cagctgaatc tctccaccca attgtttaag cttctgttca aactgttcgt atccacgata
     4021 aatatatcca atatcgctga cggttgtaaa tccttttgca gataatcctg caatgacaag
     4081 ggctgctcca gctcttaagt ctggggcgtg tacgtcagct cctgtaaatc cctcaactcc
     4141 gctgatgatc gcagttcttc catcgacctg aatggttgct cccatctttg tcagttcatc
     4201 cacatagcga aaacgatttt caaaaatact ctctgaaatc acacttgttc cattggataa
     4261 tgcaagtgtt accgccatct gtggctgcat atccgttggg aatccaggat atggtaatgt
     4321 cttaatgttt gtagattcca gacgttttgt cgctgataca cgtactgcgt catcgaattc
     4381 tacaacttct gctccaattt cgattaattt tgcagaaatt gcttctaaat gctttggaat
     4441 aacattcttg atcataatgt caccctttgt tgcgactgct gctgtcataa atgttccagc
     4501 ttcgatctgg tctggaatga tggagtactg acaatctcca aacttctcaa cacccttgat
     4561 acggatgaca tcagttcctg ctccacggat ctttgctccc atactgttta agaaattcgc
     4621 tacatcaacg acatgtggct cttttgctgc attctcgatg atcgtatttc ccttagcaag
     4681 cgatgctgcc atcataacat tgatcgtcgc tcctacagaa acaacgtcca tgtaaatatg
     4741 ggctcccttt aattcctctg ctgttgcact gatcatacca ttctcaatct caatctgggc
     4801 tcccagcatc ttaaatcctt tgatatgctg gtcgatcgga cggcttccga tctcacatcc
     4861 acctggaagt gcaactcttg ctctcttgta tttaccaagt aaagcaccga tcaaatagta
     4921 agaagctctg atcttacgga tgaattcatt gtctacatca cactctggat gaattccttt
     4981 tccactgatc gttacttcgt gttttcctgt tctgtctaca tctgctccaa tttcctgcat
     5041 cgcctgaagt aaaacattga tatctcttac atctggcata ttatctatca tacattttcc
     5101 gtctgtcatt actgccgcag ctaaaacagg cagtgctgca ttcttcgcac cgccaattac
     5161 tacttctcct actaatgggt taccaccttt aatgatatac tgttccatga tacacctcaa
     5221 ttatctctat ctatctatat tattaaacct aatttctatc tttcggtaat tatttaaaat
     5281 catacagatt atattatagc atactattag aggttttcca acactttttc taaggagttc
     5341 ccgatggata atttatccag ttttactgtg atatctgcgt atttttcata atatggaatt
     5401 ctttcattat acaggtcacg taatgtctga cctttttcta gtactacccc ccttgctttg
     5461 aggttcccaa ggcggcgttt tactgtctgg taatctagtt ttaaataaat tatagtcccg
     5521 atttctttaa aatgtctcat tgctttatca ctgtaaacag cacttcctcc aggtgcgatc
     5581 acggatttat ttacatcgat cgaagcattg acgtcttctt ccaattgttt gaatccgtca
     5641 ttcccgattc ttgcgatgat ctcttttagc aaaccacctc ctttttgctg aatcacaaga
     5701 tctgtgtcca caaaatcata acccaaggct ttggcaagaa gtactccgat cgtactcttt
     5761 ccagaacctg gcatcccgat caatatgtag ttattatttt tttctgccat ttggtttctt
     5821 ccttttcttt ttctttctta ctgagtagcc aaaggttcct aatttctttc ctgtttcata
     5881 atatccgcca tgtgaataga tcaacggatt cgttttttcc agtgcaaagc gtccgttgtc
     5941 ttccatataa tcatcatcta ccataatatt gacaactttc gccataaaca tatgatgtga
     6001 gccaagtggt acgatcttct ctactttaca ttcgatattt accggacttt ctgcgatgat
     6061 cggtgcatcc acaacctgtg cttcttcttt tgtcagtctt gcttctttga atttatcaac
     6121 atctcttcct gaacgtactc cgcagaaatc cgttgctctt gttaactgtt ttgttgtcag
     6181 gttgatcaca aattctccac tattcttaat catttcataa gaatgtctct ctgggcggac
     6241 agaaatgtac gtcattggcg gattcgtaca gatcgtccct gtccatgcga tcgtaatgat
     6301 gttatcctga cctttatctc tgcaagtcac cataactgct ggcaaaggat agatcatatt
     6361 tccaggtttc catgaagttt ttgccatatt tctctcctag agtgcctttt taatatcctg
     6421 tcttaattgt tccatatcga tgtcttctgt ggaatgttct ttccatccga tcgtcacgtc
     6481 atccccttca tatcttggga tcaaatgcat atggaaatga tatactgtct gacctgcgat
     6541 cagtccgttg ttctgtaaaa catttaatcc atcacagtga agcacttctt ttaatgctct
     6601 cgcaacaact gttgctaact ggaataattt tcctgctgtg atcgcatcaa tctcatagat
     6661 atctttaaaa tgttctttcg gaatgatcag tacatgccct ttggatgctg gtgcaatatc
     6721 catgatcact ttaaactcag agttttcata aattgtgttt gatggaatct ctccacctgc
     6781 gatcttacaa aaaatacaat cttctttctt catattatat atcttccttc ctgtgtgttc
     6841 ttattttgag agtttacata ttactctgtt tagatgtatt ttatcccatt tactattgtt
     6901 tttcaagaaa agacctctga tcaattatgc ttttgacact tatatcatat tattccattc
     6961 tcacaaaaaa tgtgtatttt gttcacaatt tggacacatt tttatgtgat aataaaaatc
     7021 gtaaagtagt taatactttc aaatcctacc cctcttgagg atgaaaatcc tctccttatt
     7081 gttttaaaag agagaaccga cctagctagt cggttctttc tttttgtttt atgattatat
     7141 aatgtaaaaa gaagccttga cttaagtttt atgtcgttga tcaacatttt tctttagcca
     7201 aagcttcttt tttattttta tatttcggta cgtccttcta atgctcgaag taacgttact
     7261 tcatctatat attccagatc tcctccaacc ggaactccgc ttgcgatctt actgactttg
     7321 atgcctgttg gtttgacaag tttgctgata tacatcgctg ttgtctcacc ttccagactg
     7381 gagtttgttg ctatgatcaa ttctttgaca tctccctgca gtctcgccat cagctctttc
     7441 agacgaatat catctggacc aattccaagc atcggagaaa ttgctccatg taaaacatga
     7501 tagacacctt cgtattttcc tgtcttctca tacgccatta agtccctcgt gttctcaacg
     7561 accatgatcg tgctgtgatc acgcttgtcg ctcgcacaga tcggacagat ctcattgtct
     7621 gtcagtgtac aacattcttt gcaataatgt acattagtct ttgcggaaat gatcgtatct
     7681 gacaatttct gcacctgatc ctgtggcata ttgataatgt gaaatgccag tcttcctgcg
     7741 gatttaggtc caattccggg gagtttccca agttcttcga tcaactgtgt gatctgatta
     7801 ctgtaataat ccatagtctc tcactctatc tagaatggga atcctccacc cattcctcct
     7861 aatccaccag tgacttttcc catcttctcc tgagaatctt cttccatctt acgaagtgct
     7921 tcatttgtag cagctacgat cagatcttct aacatttcaa cgtcatctgg atctacaact
     7981 tcttcttcta aatgtacaga aactacttct tttttaccag atacaacaac ttttacgact
     8041 ccaccgcctg ctgttgcttc atatgtggat tcttctaacg cttttgttgt ttcctccatc
     8101 tgtttctgca tcttctgtgc ctgtttcatc agctgattca tatttccacc catacctgga
     8161 aatccaccac ttctttttgc cattgttcat tcctcctgaa aattcatttt tataagtttc
     8221 attttaatct tctattttta catccatatg aatgcagtct aaattgatca tatcactgtc
     8281 atcattatct gtattcatca cactaacttc cagttcgaca tctctgccag actgtcttga
     8341 taattcctgc ttaagctgtt caatacgctc tggttcctct aagaaatatc cactttccac
     8401 gttcatttct tttgggaaca gtactcttaa cgcagttcct tcttcgttga ccgaaagtgt
     8461 tgctctctgt aaacaatact tcatctgtgg tgacagattg cgtttgacat ctctccagtg
     8521 attcacaagt tctataagtt cctcaacttt tgctggtgga aacttctcat ttaactgttc
     8581 gacaggatct tgttctgatt ttacttcctg ttgcagtgct atttgatttt gtcctgcttg
     8641 tgccgcctgc tgtactggga ttcctgactg tagcaactgt tctaactgct gttctaactg
     8701 tctcaccctg tcggccagac tgtttgtgtc tgcttccatc tgtggcctgc acatcttcat
     8761 catctcaact tccacaagga ctctcttgga tgtcgcataa cgtatacgct ctaacaattc
     8821 ggataagatt ctgatgtaac ggatcagtgt gttttcttcc acctgctgtg ctttctcctg
     8881 cagcaatttc aactgatctg ttgatacttc caatgcctcc tctgcagcat ttccttgacc
     8941 gactaagagc agattcctta aataccatag gaaatctgta ataaattgtg accattctct
     9001 tccctgtctc gccacatctt ccagaagatc catcacgcca ttgatatcct gcgaaagaat
     9061 catgtccaac agatcactat acacttgaat atcaacagtt cccagcactt ccaaaacatt
     9121 atcatatgtt aatttctcac cgagataaaa tgcaatacac tgatccagca aactcaacgc
     9181 atcacgcatg gaaccgtctg ccattcgtgc cacatattcc aacgccttat cttctgtatc
     9241 aacatgttct tgttccatca gatctgatag ctgcttcttg atcgttgctg ccgtgatacg
     9301 atgaaaatca tatctctgac atctggataa gatcgtgatc ggtatcttgt gaacttctgt
     9361 tgtcgcaaga ataaatataa catattctgg cggctcttcc agtgtcttta acaatgcatt
     9421 aaaagcacct atagacaaca tatgcacctc gtcgatgata taaactttgt actttcctgt
     9481 tgcaggtgaa tactgtacct gttccttgat ctctcggata ttatcaacac cattattgga
     9541 tgctgcatcg atctcgatca cattcatgga acttccttct gcgattgctt tgcaaacttc
     9601 acattctcca catggttccc catcgactgg atgttcacag tttactgctt tcgcaaggat
     9661 cttggctact gttgtcttac cagtacctct tgtcccacaa aacagatatg catgaccaat
     9721 ccgcccagtc ctcatctgat tctcaagcgt cttcacaata tgatcctgac cttttacttc
     9781 actgaaacgt gttggccgga actttctata taaagccatg taagacatat catatgcccc
     9841 cttctatttt gaaatatgac atatagccat acttcattat attttaccat gagtatctat
     9901 cctttttcaa tgattgtttt ttgacagaaa acatcgaact tttttgaacg ctttttcttg
     9961 cccttaatct tattttttcc gataataaaa gaggaaattt aattacaaga ggagccaaca
    10021 atgaacgatt tacaagaact ttctacgttt tcacatgaga taaacaattc tctgactctc
    10081 atctacggac agattcagtg cattgaaaaa acatatgata cacttaccgg aaatgaccat
    10141 tggaaccgta tgaaagatga tttttcttct ttatttgatt tcattcatca gtttctggca
    10201 ccagcagaag aatcttctgc cgtcatggaa ccactcgacc tgatcagcgt gatcagtgaa
    10261 atccgttctt cctggtctta ttatctccat aaagaacaga ttcgcttctt tattcaggca
    10321 gaccccggaa ccgcttttta cgtttacggt gcaagatcaa aactttttca gttatttcat
    10381 aatctgatct ccaatgccgt caaagcgatc caacaaaaaa aggagacctg ccgatctcct
    10441 catattacac atattacagt ccacttaagt aaagaacaag gacatattgt attaaattta
    10501 tccgatactg gaatcggaat gaccacagaa cagcaatcga gagctttcta ccgtggtgta
    10561 tccttccatc ccggcggaaa cggaattgga ttatccgttg tacaaaatat tgctcgcgat
    10621 cttcatatta aaatccatat tgattctgca ccgaatcagg gaacaacttt cacactgatc
    10681 ttccctgctt atgagcagca attaaactgc ccaacacgta cagaagcatt gacaaaataa
    10741 atccagcaat acatcctccg atatgagctg cattgtcggt tgttccactc tggaatccat
    10801 gatacagact tcctgcgatc attagaaaga ttccaaatcc atcgaatctc tgtcgttgtt
    10861 ctttgcagaa taaaaatatt gctgcaagag caccgactaa tccaaatatc gcaccagaag
    10921 ctcctgccga caacacatct tgccctatca tgctgtaata tgctacagaa atgatacttc
    10981 cggcaatgcc tgctccggta taaagaatga cataccgcac acttccgatt actttctcca
    11041 aagaagatcc aaggatcaaa aaaactgcca tattattaaa gaaatgatcc catccaaaat
    11101 gcagataatt cgacgtaaat agtctataat attcatgcag tttctttacc atatatgcat
    11161 tacaggcaaa tgaatcatag atcttgtctc cataagtttc tgtcagaata aaacagatca
    11221 aattagctgt aaagatcaga agggttgctt tatattgata gagcgatttc cagaaatcct
    11281 tgacactttt attatctttc acttctattt ccgatccttg ccctgcttga cttacctcat
    11341 tctgtgctaa tactatggat aatggtccat acagatcatc gaactgatcc tttggatcaa
    11401 gaaatactgg tttatcattc tgcgacaaga taagcatcaa tacaacacaa tccataccaa
    11461 acagctgcct tgcatagtct tccaaatgat acagatcttc ttccaaaata ctgcttccat
    11521 ccggattctt gtcaaatatc acgaacacac atttatttct tccattgata ttctttacat
    11581 aacatggtcc tgtttcggta tcatttttca tatatccatg aatccttaac aattctatga
    11641 ttttctgata cataatttcc tccgctttga tttatgtatc tactattata acacagaggc
    11701 tagtcttgtt taaaataaaa ctcctcaagt ccgatcttta tgcaatctcc ttcttttaat
    11761 actgcttttt tattcttttt cccatttaat tttacaccat tgatctttat accatttgtc
    11821 gaatcgagat cttcacaaca tacatttcca tcttctttat aaaatcttat atgtctccga
    11881 ctgatcccag ttccttctaa ttgatgatct gttccttgtg cggaacttcc aatgacaaat
    11941 ccattatcat ctaatacaat ttctttttgt tctggattct tcggaaccag tcttccaatc
    12001 ggttcttcca gtaaaagaac cgtcctgtca tcctcatcct ctaacagaat tgttttctca
    12061 tccaataatt ctgcagcatt ttctgttttc tgccaagaat ccacaaaggt ttttggattt
    12121 ttgttctttt tccacaattt ataacatcca tacacactga caccaagacc tgcgatcgaa
    12181 actagtgatc cgatcaaaaa caaataataa aatccatatt caaatatttt taatacaaaa
    12241 taaacaattc ccaatatgct gcaaaaacaa ccaacaacac ttattatttt cccgatcaga
    12301 attaaaattc ttttcggtgt tttttcataa attttgattt catctttttc aggagaatct
    12361 ttctcatctc ttataagatc cgatagattc tcctgcatat tttgtgctga ttttgattct
    12421 tcatttttta ttttttctct taaaatttca ccattcattt tctctgaccg aacttccaga
    12481 taatctttca tctgctccat attcggtttt tctttaataa gaaagctgtg aaattcataa
    12541 taaaaacgaa ccctcttttt atcatctcca gaaatatact cgatacattc ttctataaag
    12601 tcacgaagct gctcatcaat ctttttttca tactccggca gataacaaag aactggtcga
    12661 ttttctttat ccagaaacat agattccaaa tctaatttta aatgatccag ctctaacaaa
    12721 taggaggcta cctgttcaaa aacttccaaa agatacgcaa aaaacaattt cggttctaac
    12781 atagaaatcc tgtcttttaa ctttgtgtaa atacctgttt cataataata atattcttct
    12841 ccatcttcga tccggaattc acatggaaga agacacggaa gtgtatgatt gtaaagaata
    12901 tttttttcat atgttaattt ctgttctttt ttaacaacat aataactgga aaaaccttct
    12961 ctaatccatt gctccttcaa gtgcattcca cctcctgatc tgatcgatct caaaatctcc
    13021 ctgtgcctga tcatttcctt catattcaaa caaacagatt ccaaacatac ttaagaaatc
    13081 acctgccaca actgctgatc ttgtctttat ttttgctgaa actctaagcg gtgttgtcga
    13141 tactctaacc gaactgactt ctgccataaa cagatgcctt cgcaattgtt ctttcatctc
    13201 tttttcagct tttgtatttc ctgacttgtt tgacgatatc acatttccag tgatcagctt
    13261 ttgatttact ttctgatacg acaactttcc cgtaccatac tctgcctctg taattttaac
    13321 atctgccgct ttctgcactg cttcttccat aattccttgt gcgatcccac aattcataaa
    13381 ataaaacaga tagctaaaaa acatccatag caccatcaaa atgatcggaa gcagcgcact
    13441 gatttcaacc gtcatgtatc cccctgtttt gcgcatctcg tgcatggcct catccccttt
    13501 aatgtactta gatctacttg tttgactgtt ctttttaacc cgctgcagcc aagatccgtg
    13561 tgataagatt ccccttcttt tgcgatatat acacaggaaa aatcaccttt ttcatgcttg
    13621 gtgcatttat cacatggctt gtattgtgtc cgcttgtttc tatatttttc tacatcttcg
    13681 tcaacagaaa tatccaatga cagatgtgta caggagagat ctttatggta caccgcttca
    13741 tgcggtgtga tatagacata gccttttttt aattcatctc cgtctggtac atatcctgtc
    13801 atggattttt gccttacact ttgctcatag gatactgtat gtgttgataa aaacggcatt
    13861 gttttgcgaa tataataacg cacagatacg atatattccc ctttagaggt caatgtcagt
    13921 ctacacgccg ctgccgctcc tgcacttcct ccgatcaaag ccgaatggtc taaaaactta
    13981 cggtttaccg ctgccagaaa agctgccttg gcactaaatc ctgataagtc acttccagat
    14041 tttttcttct gcctgatgcg atattcatta actgcagccc tctttgctgc ctcacaagca
    14101 ccatgagaaa tctgttggtg gatcatcaaa atctctcctg cacttgtaag catcaaaaga
    14161 acgatcaaaa aaatggggac gatcagactc gcttccaatg tcattgcacc ttcctcattt
    14221 ccagaaagat ctgaggcaga ggaagatgct ctcttggatt tttttacgac gatcttgttg
    14281 ttattgttat cattttgaac cggattcact tttgagaaat gtttttgaca agagaacatc
    14341 tttctctgcc ccctttcttt cattttgatc atttaaattt taatacgtta catatcgata
    14401 cattttaaga tctgtatatg ttgcgaatgg caagatcatt ccaccaaatc cgagtttttt
    14461 caacttcaga ttctgcgtga tcttgtaaga agaaatgcat tgatccaatt taaaatctga
    14521 cacttttctt cggatatttt gttccatcag atccatcatt cggaaatatt tttgacgttt
    14581 atctttttgt gatatcaaca gtaataacaa ataatcctcg tatgatattc cctgtttcac
    14641 aggttttctc ttgcaattta acgttgccag tcctgcaaaa gaaagctgcc agtctgaaga
    14701 tgtttttact gctgggattt ttttaccatc tgcaagattt cttacatcga tcactgattc
    14761 cccataagca agtgctaata ataacagatt ctttgcaacc gcaactgctg gtggaaatcc
    14821 tgtaactcca agaattgacg ctgctaacaa tgctgcttct ttttctttgg ctggatcttg
    14881 atatacacaa gctgcattca tcacaaatct aagtgcgatc atcctccaga aaaccattcc
    14941 aagattctct ttttccgaat ctttccctgc gatcaaatat tcaatctcat atttaatccc
    15001 gtcttttttt gttgtatctg tcagaaaatg aaaatatttt gttgcatata aaactaccgg
    15061 taattcactt gcaaaggaat ccgttgatgc tgttttttcg aattcatcta attctttttc
    15121 cacatctttt cggttcataa aattccatgc actctgcttt gttgtgtctt ttttgccata
    15181 gacaatatta attggaagat ctgacacttt tttctctccc atgatcaatg ggaccgagcc
    15241 ttcttttaac aatttcataa atccttttct cggatctttt tttactggtt ctgatgttag
    15301 tgttttttca ttttccgttt cttctgcttc ttcttcctct ttttgaatct gatccttaat
    15361 tcctgagagc tcatttttct ggtcttctcc agccttgatc tttttggtca agtttttcaa
    15421 aatatcccct gctgcttcat attgcatctg ctgccgaatc tgatatttta aaacttctcc
    15481 tttttgtgcc gacagatcaa gtatatctga aatttttgta cttcctgttt gaaagcgaaa
    15541 aggatctcca ttctgatcca gactttcttt catgcggtct gaaaaatctg tttcgatctt
    15601 cttgtgatat cttggatcca tcgcataaat atgatacata tccgcaagct ctttctgata
    15661 atttcctttt gtatgagaaa gcccagacat ataaataccc atcgcctttc ccctcgctgt
    15721 atacatatag attccttcca cacatacgct gatcaacagt aaaaaaacaa gaaataacat
    15781 tgcaagaaat gcactcatac taccttgctc atcccggatc atttgacttg tcctgtttta
    15841 ctggtgatcg ttttaaaaat gctatcaacg actttcttga tctgcgtctg gaaaataatg
    15901 acgagtccga tcaaaactac aatgatcaaa atcacctcaa caacacccat tcctgattca
    15961 tctttaaaaa aatgctttag aaaatttctc atatgtttta ccccctgacc ttttatgaaa
    16021 atgtagatag tgctggaaac atcaataata ctaaaataac aaccaacatt accatcatcg
    16081 gtgcgagcag ttttgtgctt gcttgttctc ctttcctttt tgcttgttct tttttctcaa
    16141 aaaaagcccc ttcttcctct tcttttaacc ttagaaacag atccgttgaa cctcttgtaa
    16201 tactctgtac gagcagaaca gataattttt gataagaaga caacccaatc tttttgccca
    16261 tctcaccaaa tgcttttgtc tgtggaactc caaatgaaat ctgtctgcat gtttttttga
    16321 tctgttcata tacgaagatt tcttttttgt ttcctcttct ttgttctttc tcgtattccg
    16381 tcgctactgc ttccaatgca gccaccacac tcattcctgt ccccagaaat aagatcagat
    16441 gttctatgat ctgtggatac tccaccatag attgttccaa tgtctgtatt ctttgttcct
    16501 tatatttttc attttcgtga taccagaaaa atatgatcac tgcaatccca agaaataaaa
    16561 ttgcaggaat ctgactttca ttttccgcag gtaaaagtct gccctgccgg atcacatctg
    16621 gaagataaaa ctgctccttc gtaatatgtt tttcttccaa tttcttaaga tctgcaatga
    16681 tcttttgttt ttctgatacc tttttccctg aattcccagg aactacacag acaggaatct
    16741 ttaagatcct tacttctcct tgacattcca tccttgcatc aagattcaac agatagtctt
    16801 ttttgacatt cttcgaaaac acattgcctt cttcatcgat gactgtagga tcatcactct
    16861 gccattgtac cgcaatattt tccccaggta tctttactgg catttcaaga tttcgttcca
    16921 cttcatttaa tgatttattt ttcgccacaa gatgtttcct taaataagct tctcctctgt
    16981 caaatgcctg cgtgatctgg gcttttgtat atccttttgg atgaatctcc aaggtgtaaa
    17041 tatcatcatc gctttttgtt ttaatacgaa tctgttgctc tgtaacttcg ccgcccgttt
    17101 catctctcgt gatcagaatt ctacgatcct gactgaaata aaagattatg gtcagaaaaa
    17161 gaacgataag aaaaataata gtcgccacgc ttatgatctt cacctgttca tacagactct
    17221 tccttacttt ctcatctaat tgtttctttg ttcctatgaa acaggatcct atctctgctt
    17281 ttcttttttc tttgagttct tctggaagtt tttccacgat cttattaatc cattgaaact
    17341 taaatttcaa tctcagtcac cctctctgcc catatatctg cgatccacag acataaaaga
    17401 gaaatcactg cgactaaaac tccggcaaaa ctctcataca gtatgtcgaa atatccagga
    17461 tttgcaatcc ttacataaag gagaataaaa tatggcatga cagacatgat ctttttctcc
    17521 atttgttttg ccgcgatcat cgtgtgaatc tcttcttttg tctggattgc ctgacttaaa
    17581 tgttctatcg tattttccgt gatctcaatc agattcccac cacttttttt cgcaattgat
    17641 atgacctgtg caaactgata aaattcttcc agacctacat gttccgccat atcaaaaagc
    17701 agattatcca caggttcgtt cattttgagc ttgtttatca acaattgaag ttcttttgcc
    17761 aacaaactgt gttcttcata catctccaga tcattttttg ctgttttcaa tgcattttca
    17821 atagagtatc ctgcagataa actatttcca atagaaatca tcatttcacg gaattccttt
    17881 cgcagctttt ccttctcccg atgcacctta tggattcttt gttgtttcat aaaaacaaac
    17941 aaatatggaa tcaaaagaat cgcaaatgca attcggtcaa aaaacagcca tgcgatcatc
    18001 acgatatagc aaattccctt aaccaactct tgcttctcat atttccacag gctgatctct
    18061 tgctttaaaa gattccataa gtttctgata tgcttcatac tgaccatact cctttaactt
    18121 cttctgatca cataattctg atctcattgt aagttcatca tcttcattca tctcaaacaa
    18181 cggatgtaat gctacctgac tcgccgccat accagtaatc tctgaaatct ccatcagttt
    18241 tcgacttcca tcaaagcttc ttccaagatg aatgatcaca tcgattgcag atgccatctg
    18301 cccacggatc gcttctactg gcatatcgac tcccatcaag accatcgttt cgattcgctt
    18361 aagcatatct ttacagctgt tcgcatgtcc tgtcgagatg cttccatcgt ggcctgtatt
    18421 cattgcattt aacatagaca aggcttcttc gcctcgcact tctccgacga tgatccggtc
    18481 tggccgtgaa cgtaatgccg ctttgatcag ttctttcata tcgacctgat tttcccccgc
    18541 tgcattcgca ttcctcatct ctaaccggac cagattatca atcccattaa gtttcaattc
    18601 tgcggaatcc tcaattgtta tgatcctctc atctttcgga atacaatttg acattccatt
    18661 aagcaacgtc gtcttcccgc tgcctgttcc ccccgagata aaaatgttat agcgcgccct
    18721 tacaagaatc ttcaaaaact tcgcaatttc ttccgaaaat gcttcccgtt cacaaagcca
    18781 tgctaatgtc atcggttcct ttgcaaactt tcggatcgtc atcgttgccc catcgatcga
    18841 tatcggcggc aaaatgatat ttactctcga accatcgtta agtctcgcat ccacaattgg
    18901 atttcgttca ttcaccatac ggttcgctcc tgatgcaacc tgctgaatta cctgatataa
    18961 tttttcatta tccgaaaaat gcccaggata tcggtgcagt tttccttgcc gctcaataaa
    19021 aatattttta tagccattga tcatgatctc cgtcacagaa tcatcttcta acagatcttg
    19081 cagaatatcc aatcttcgaa gagcataaaa aacttcctga ttcaagcgaa tcttgtcaga
    19141 caaaagcaac ggatattctt ctgatatttt catgatctct tcctgaatca caccttttaa
    19201 atgttcgtca gaaatctcct gtgaaaaatt caatttctcc attactcttt gtttcagcaa
    19261 ttccttcaag gccactgccc ccttcctcct ttgttcttcc agctctcaag catcacttct
    19321 tcccagtttg tcaatttcca tactccatgt tttctcatct gtttttgaaa cttcttatat
    19381 tccatacacc ccagatcttg ccgcataaga ggaagaaaaa tccggtcaaa caacccaaag
    19441 aacttaagat ctggcggtga tgcgatgcca atatcaaaaa taatacaaat tcctgtttct
    19501 cttaatttct caaaaaacca ctcataatcc tgcatggata attcccgata atccagataa
    19561 catgaagctc ccgcaaatcc tacacaacca tcaaacttct tctgatgttc cataaaatat
    19621 agcacaatat cttctttgtg ttctttgata taaaacaaca aaaggtcatc tgtctcatct
    19681 tcttcaaagt gtccatatgg ttgcattcca aaataaatcc acccattcga agctgccata
    19741 ttccttgcaa atgccgatat ctccagtctt gaaatcggag aatatactcc aaaaatccgt
    19801 tcattttcaa tctgaggact tacattcagg caatgataga ttcccgctgc gatctctttt
    19861 gcactctggc aatacatccc acccatagat tcatttgctc caattcggca aaccggaaaa
    19921 tctccatgat aatccgtctc ttctgccagt accaggcatt caaaatctcc ctcttgttcc
    19981 tgtttaaaaa acatctcctc atccgacgta aatgatgccg tcatcgggaa atctttctgg
    20041 tgatttaagt attccatcag ccgttttcca tactgatggt ctttgtgata aataccgatt
    20101 gaaatatcct tcatagtcca cctcctaaca ggcagatcat gtacgctgca aggatcccaa
    20161 atgaaaaatg tatcattcca tctttcatca gatccccatt ctctgtctcg tacatcaata
    20221 atctcttgtt atccttacat tgtgttacat attgaaataa gcgaaataat cgcagatgga
    20281 agttcttcct cttccataaa aagatcacgg cataaatacc gttacatata aatgacaaaa
    20341 tcagaaaccg aggcattgcc cgtccgatag atattgcaca taccatcagc agcttcacat
    20401 ctccaccgcc aaggcatcca atatagaata ataatccaca gctgatcagc acaaccaaaa
    20461 ctccgatcat catatctgct gccttcatcc cagagactct tgcaagaacc agcgtgatca
    20521 acgctcctgc cccattgagc ttattcggga tcttgaaatc tctgtaatca taaattgctg
    20581 ccatgatcaa aaataataga atccatatct tcatctttcc tccttccttt atgttccatt
    20641 attttacatc tatttacagt atttgtctag tattttttct attattttac agaaatacaa
    20701 aatccagtat tttactagcg cactttacat cacattatta ctgcaataaa aacaccgctg
    20761 ccgcatacaa cacaataatt cctggaagcc ccaacagtgc acctgttcca atggacaaga
    20821 tattaagatt gactggtgct gtgattcctt tccgcataca aacaatactg attccatata
    20881 cgatcatcgc acaaaaagct ccccgcagca aaagcactag caaatatgcc ggtttcttta
    20941 caattccgat catcagacag atcatacatc ccgctgccac tatgtaaaag atcatttcat
    21001 tttgtcccat aaaaaaatcc ctccttatgt aatcttatgt tacataagga gagatattcg
    21061 tttcttatat gctcctaagc ttttatgaaa ataaattaga cagccagctg aaaatagact
    21121 ggaagaaagc tgcgatctta tcaaagattc cctgacttcc tccaagctta tcatataaat
    21181 cttttgcttg agattttaac tgagacacat tcagatcaag tcctgaaatc ttatccatca
    21241 gactctgtat cttcgctcgg tctgcatcag aaagattgat atttaaatct tttgcagact
    21301 cttcaatcgc ctgtttgatc tcagatggag atgacagatt atcttttgca actttctgtt
    21361 tgacatctgc gatcagtttc tctgcatcgt cagaaccgat attttccgca acttctcccg
    21421 ttgtgatcaa ctcgtctgtc gctgcatctt tgctcttttc tggaatcttc tttcctgtca
    21481 tttcttcgta ggctttcata acgccaacca atgctgctgt tccagaaatc ttaaatggac
    21541 cagctacttt tacatcggca ttcttgatcc ctgctgtgat cagagcattt cgatacattc
    21601 cagatgtgca gtaagagata ttctgtgttg tgacattcac tccatttcca tcatctttct
    21661 gttctacagt cacagaagat aatgctcttg ttccgatcac tctgcttgat agatagctgt
    21721 ccaaatattc atgttcatct ttatttgtta cagtaataac tttatagtcg ctattatcat
    21781 ctaaatccag aagttctaac actttctttt tctgggaagc tgttaagtca gctccaagtg
    21841 ccagatattt ctgatcaacg ctgtctgctt tgacagttac agatgataat gccattgcga
    21901 cacaaagtcc tgctgcaagg aattttccaa taaactttct cataaatcct tttcctcctg
    21961 cttattaatt tctttcatca tagcaaaaac atctctgact ttcaatatgc caaaatcata
    22021 agaatttctt acgaaattta tatttcttta actgcagctt ttttctttct tctgaaaaaa
    22081 gccgtgatca gaataaaaat aacaaatccc acaacactta agattgcccc ttccttaaac
    22141 ccagaagata catacgttag tgaaacttta tgctttcctt ccggcacttt cactgtcaaa
    22201 aatgctttcc caagtgcttt tgttttcact ttcttgccat caatcttcac cgtccatcct
    22261 ttatcgtatg gaattgagaa aaataacatc tgatcttcct gtgcatggat cgttccactt
    22321 agatgatttc ctttggcact ggtaagtttc catgcatttt cctgcattct ctgtgcaagt
    22381 tcttccatca cttcttcatc aagttccgct gctgtcagtc ttacaactcc agacatttca
    22441 ttatcttgtt tgagttgcat tttgatgcgg atctcatcac ctttctgcac atttccaaga
    22501 taaatgatct gtgaatccag ccttcccgtc aaaacctgtt ctccattttt ctcgatcaca
    22561 gtattttcta cctggcttcc atcaaaatga atatacaacc gccttgtttt tgttgatctg
    22621 atcgtaaata ccatgttatc tggtcgggcg gatgtatttt caaatacata ttctccatca
    22681 ccattatcgg atgtgatctt acaatcgttt aattgcggct tagctgtttt gaccatatga
    22741 aacaactcat caacatcaaa tgcactcgtt gccagatcat tttgtacata aaatggatta
    22801 taatcttcat aatcccaagt ttgtaccaag tcatccatcg tataggcaag tgctgttcga
    22861 taaggatttt tataaaccgt gacatttcca acactttctt ttagtgaaaa ttcggtatat
    22921 ttcgtatcat atggacggta aatctgatat ttcatagaca tcagattatt tgtaaatggc
    22981 gttgcacctt catataaata ttcatttaca cccgtgtaga atccaagctg atccatcata
    23041 tcgacaacgt ttccctgtgc tgtggaacca aacaaggtca ttccatttaa ggtatgccag
    23101 atactttcat caagcatacg cccactgatc aattccattc ttgtctctac atttttctga
    23161 tactcttttt ttacttctgt gatagctttg gtatcctgaa aataactatc tgtatcgacg
    23221 gttccatttt tttgaaatcc catcactgcc atcgctgcgg tttctactat aaataagaaa
    23281 cagatcaatg ttgtaaatat tttcttcctg aaaatcttct tctgatataa tgcgaaacaa
    23341 atcatataaa ctgcgatcgc tgcaactgtt gcgtaaaccg ttttcatttc caatttcatt
    23401 gtcgacgttt tcattgtgat tccaatcaaa ataccgcata ggatcactga caaataaatc
    23461 cacagtgaaa cttttctttt gcctcttacc aacacacaat atccttcata cgccatcgca
    23521 agcaatgcaa aaatatacaa aaacgcaaag cggtttggaa ttccatactg atcatgaaaa
    23581 ccatgccaaa cataacctaa gacaggcata ttgaaactaa aaaataaaaa tacaagtaat
    23641 aacaatcttc gaatcttatc gatcaggcga atttctctaa ctgccagata aaatcctgcc
    23701 attaataagg ttaagattcc acaatataaa ttgatcttac tgtcatctac cgccatcgta
    23761 agcggtgttg tcccaagaaa atgtctggaa aacagatttc caaatgttcc ataccataat
    23821 tcttttggaa attgtaattt ggctgaggat gtctgcnnnn nnnnnnnnnn nnnnnnnnnn
    23881 nnnnnnaacc gctgccattg ctccagataa cagtgaatat cctgcaaagc ggaatccatt
    23941 tgtaaaaaat tctttgacat ttttatgtga atatagtaaa taccacaaga ttaaaaataa
    24001 acatgtcata taggacatat aaaagttaca ccataaagaa ataaataatg caaaacaata
    24061 aagtcttcca tcatgttctt tgatcagctt atcaatacca tacaaaatga gtggaagcat
    24121 catcatgact tccatccaca taatattcca actgtagcca accatataac tgcataatcc
    24181 atatgccatt ccgaacgcaa caatggatat ctcatccttt ccacggcgtt ttctaaaata
    24241 aaatgcggct gtaaagcagc acagtgcgat ctttaagaca tacaaatgac ttaatatcat
    24301 cgtaagcatt gatttcgaca caagtgcgat caccagattt agtggacttg ccagataata
    24361 cgcccatagt ccaaggaaat tgtatccaag acctgcatga aaagaataaa acatcgaatc
    24421 catgcttttt aatttttcct gataatctgc aaaaaagggc aaatactgat gcaatgcatc
    24481 aacgatcaga aatgattcac tgccaaatgg ctgtatcttc tctgcgatcg cgattccttc
    24541 taatagaaga accggcagaa aaaacgagag taacagatac ttatgagttt tcagtatgtc
    24601 tttgattttg tttgtcatgt ctgctccatt cttttacacg gtgattgatt cccatgatca
    24661 tatgatatct ttttttgatc atggatgtga tcgaaccatg ggtcaattcc gtaacatttc
    24721 ctccatgtct tcgataataa ataagaggtt cctccaaaaa tacaactttc ccgagttgtt
    24781 ctgacagcaa tccgatccat tggtcatgaa tccagatgtc atctgggatc ggcagaattc
    24841 ttttcattgc ggatcttctg actgccatac agcatccgat ataggaattc tttttcatat
    24901 tcttccagaa gcctgttccg ctatttctcc actcaaaagt ggtttgatct aaagatgaaa
    24961 gtttttcatc tacaatctgc gcatcatgaa caactgccat cactttcggg tcttcaaata
    25021 cacgctcaat ctcgctcatt ttattttttt tccacacatc atcctgatca gataagaaga
    25081 tgatctcccc tcgacaatgc ttaaatgcat tctcaaaatt ttttacgaca cccttcccag
    25141 gtcctttaat tatgcggatt ctttggtcgt tttggaccca atcctcaagt attgcttctg
    25201 atccatcact agcatcatcc actgaaatga tcagttcatc tgtctcttcc atattatcaa
    25261 gaatcgatct tatttgctca tctatatatt tcgcgccatt ataaaaagcc attgccacag
    25321 atttcatact aatttccttc ctgcgtattg attttcttga aatattatac catatcttat
    25381 ataataattc taagacaaag gcgtcattga gaaaaagagg cgttttccga cctctttttg
    25441 ttttatcttt tgggatgtgc caacacccat ttgaagaaga taccctctgt tttaaacaca
    25501 catatcatta tgacttataa ggaggaaaca aaatgtcata cgtagatgaa gtgatcgagc
    25561 aggtagttgc tcagaaccca gcagaacctg aattccatca ggctgtaaaa gaagttttag
    25621 aatcattaag agccgttgtt gaagcaaacg aagaagcttt cagaagagat gctcttcttg
    25681 aacgtcttgt aaacccagaa cgccagttca aattccgtgt accttgggta gacgacaaag
    25741 gacaggttca tgttaacaca ggttaccgtg tacagttcaa cagtgcgatc ggaccttaca
    25801 aaggtggttt aagacttcac ccatctgtta accttggaat catcaaattc ttaggatttg
    25861 aacaaatctt caaaaactct ttaacaggac ttcctatcgg tggtggtaaa ggtggatctg
    25921 acttcgatcc taaaggaaaa tctgaccgtg agatcatggc attctgccag agcttcatga
    25981 cagagctttg caaatacatc ggagcagata cagacgttcc tgctggtgat atcggaactg
    26041 gtgcaagaga gatcggttac atgttcggtc agtctaagag aattcgtgga ctttatgaag
    26101 gagttttaac aggtaaaggt ttatcttatg gtggatcttt agctcgtaca gaagctacag
    26161 gatacggact tctttacttc acagacgaaa tgttaaaatg taatggtaaa tctatcaaag
    26221 gtgctacaat tgctgtttct ggagctggta atgttgctat ttacgcaatc gaaaaagctc
    26281 agcagttagg tgctaaagtt gttacagctt ctgactctac aggatggatt tatgacaaag
    26341 atggtatcga tgttgaatta ttaaaagaag taaaagaagt aaaacgtgct cgtttaacag
    26401 aatacgctgc tgctcgtcca agtgctgaat accacgaagg aagaggagtt tggtctattc
    26461 ctgttgacat tgctcttcca tgtgcaactc agaacgaatt acacttagaa gatgctaaac
    26521 agttagtagc aaacggatgt tacgcagttg cagagggtgc taacatgcct actacattag
    26581 aagctacaga atacttacag gaaaacggaa tcttattcgc acctggtaaa gcttctaacg
    26641 ctggtggagt tgctacatct gctcttgaaa tgtctcagaa cagcgaaaga ttaagctgga
    26701 catttgaaga agttgatgga aaattacaga acatcatggt taacattttc cacaacttag
    26761 atgacgctgc aaaacgttac ggaatggaag gaaactatgt agctggtgct aacattgcag
    26821 gattcgagaa agtcgtagat gcaatgacag ctcagggtat tgtttaatcc ttaagaagaa
    26881 agaagaatgt tcccgtggga cggaatgttc aattaaaata atgtcatgtg tatgtagaaa
    26941 gcggagatcc ttttatggat tctccgcttt ttgggtttta tttatttgct tttaatttcg
    27001 gtgttcttat gatctgtacg agcaatggaa taaccatgat aaaccatacg atcggttctg
    27061 ctacgataac acctgcatat cccatcaaag gaactaatgt aaatgcgatc accacttttc
    27121 cgaccatttc taatccactg gatactaatg gtgtgatctg atccccgatt ccttgcataa
    27181 catttcttac tatgctgatg accgctggca cataataaaa gatcgtatta aacttcaggt
    27241 aatttgttgc atttacaatg actacatctt tgtgacttcc tgtaaccata tggactaact
    27301 gcggtccgat cgtataactt gcgatcatcg caaaagttga ccagacacat gtataaatga
    27361 tcgctagttt catacctttc ttaattctgt caactttccc agctcccata ttctgaccac
    27421 agaatgttgc catcgtctgg ccaaagacac tgaacatgat catatatatt tctgtgatct
    27481 ttcttgccgc tgtatgggct acgataattt cctgcccaag cttattgatc gctgtctgta
    27541 atgtaagtga tccaatattc acaagagaac tcataaatcc catagacatt cctgtcgcca
    27601 gcatttcttt gatcatttta gattccagat ctttaaaatc atttttattc agacgtaata
    27661 attcgtactt atgaatcata taaaaacaac aaatgaccaa tgcgataaac tgtgccaaca
    27721 cagttgctgc tgccgcaccc catgttcccg ttttccatac tgcaacaaat aaaagatcca
    27781 gtacgatatt taatcctaca gaaatcgcaa ggatcaataa tggtgttaac gtatctccga
    27841 tcgcacgcat cactgccatc aaaatatcat aaaacatcgt gacccataaa ccaatgataa
    27901 tgatcagtat atattgtttc gcttcgataa ataatttacc tggaacattc aaaaatccta
    27961 aaatcggatt gataaatacc gttccgatca agattaaaat agcagaaacg atcactccta
    28021 acacaagtgc gatcgcaaat gatcttcgaa ctctcttttt atcacctgct ccaaatttct
    28081 gcgctgtaat gatcgcaaat ccattggcca gacctaataa gaaaccaacg atcaagttac
    28141 tgatcgttgt cgttgcacca actgcagcaa gtgatcgctc tccaagaaaa cttccgacaa
    28201 ttcttgtatc agcaagactg tatgtaagct gtaataagtt tcctaaaaat actggaatcg
    28261 caaatcctaa gatcaatgtt gaaatctttc cttttgtcaa atctttcatg atcgatcctc
    28321 cgctttctgt aatcttccat ctccatcgat cacaactttc attccttcat taatatctct
    28381 ttcgtatagt aatttatctt ccatgaagat tattgtttct tccttcgtta tttcttcttt
    28441 taaatatact ttatctttga tttcctgtcc ttccgggcgg ccttcacaac cgaagtctgg
    28501 ttcttcgatt cttgtgacgt agtatgtgtc catatattgt ttttccttct ttctttgtta
    28561 tatttgaaaa ttttgtaaaa taaaaatcat ctattatata attatcacgt catcaccatt
    28621 cacataattt tgatcaccta acacggttac ttccccatat ccatgcagtt tataaatccg
    28681 aatattatcg gttactcttg tcatctcttt taattttgaa agtaattttt caaaatgttt
    28741 cttagtcagc cacgcttcaa atgcagattt ttgtattctt gttccatagc cttccaaaag
    28801 ctttgatatt ttcaggcgat gtttattatc agatatatca tatatgatca atatcagata
    28861 tttgtcattt ccttcatctt ctactagtgt ttcaaattca aaataatcca taatcatcgt
    28921 atccttattg gcatataatc atcaaaattc ccattgtcaa tgcattttac tattttagat
    28981 acctgccacc agattgctct acgaaagctt actgggtgtt ctagatattc taagtaattc
    29041 atattacttt gcaatttttt ctctagtttt tttataaata gattgacacc atttttacta
    29101 ataattacag cacctgtttc ttcatctttt gaaaattcat tgatactgat ctcatttccc
    29161 tgaatcatac tcatgactgt tgcatctaca atcaccgctc tccattcttc caaaagatca
    29221 ctggcaagtg ttggatgtct ttcaatatct ttatgcataa atcctatata tggcgttatt
    29281 cctcgatttt ctatttcacc cataatctca tacatcaaga ttgtatatcc taaactgatc
    29341 atcgaattaa atgcatctcg tggtggacgt ttatttcgcc cagaaaattt aaaatcttct
    29401 tttatcattt tggataatgc tgcaaaatat tgtctggcag catttccttc atagcccata
    29461 atctgttcaa tatttgtaca atgtgagatt ttattttcat gtatttttat cgcttgaata
    29521 ttttcttgaa tattcttccc cgtactgcgc tgatatcttg tcaaaagaac tctttgatta
    29581 tgtatcttat ttaaaataat cttttttgaa aactgcactc gcatttcctc ttgatcactt
    29641 aaatatactt gctgtttcaa tctctttgca ttcacatgat ttgttgattc aatccgccca
    29701 aaatattttc cttttgttga aaacatggat actgctattc ctcgctccag acattcctta
    29761 atacatggta ctgtcaaata aacatttcca aatatcacaa ttgattcaag cgtttctttc
    29821 ggaacaaggc gttctgatcc gtcttgacat tctacaataa aatgcccacc t
//
LOCUS       DAOMUE010000003        29741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_191_length_29741_cov_2.841609, whole genome shotgun sequence.
ACCESSION   DAOMUE010000003 DAOMUE010000000
VERSION     DAOMUE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 29741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29741
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191_length_29741_cov_2.841609"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PLC71_00365"
     CDS             complement(<1..606)
                     /locus_tag="PLC71_00365"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HRM01132.1"
                     /translation="MSGTKISSDDYIFESDVVYHSGQAFALLFRGQNPDSTSAYAANV
                     DTQRKGSTARIFTFGGGTGDIGKDGNYNLSEGQKYHFKVVVKGNQYKVYIEGKLVLNV
                     RDVKVENNYKEGRYVGFNVFNGKVTFQNMKVTPLEVKVPTVSDKLALTAGEKAAIDVS
                     NIEDNSFNIIKYKSLDKTIADVDEDGNITAKKAGTTQIETQV"
     gene            complement(606..3422)
                     /locus_tag="PLC71_00370"
     CDS             complement(606..3422)
                     /locus_tag="PLC71_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HRM01133.1"
                     /translation="MRKVSPRLVAIMMASVVSVTSIAPVIAYNHVTVNAAQLQHQAID
                     WTFTGNGKVDNDKIQLSGAGDNIGITNIKKEDNYAVKAKLTQGEEDGAVGVIMGAKEA
                     KDPKKGSVVANVTPSTGAVRLICFQGGRNNDLQKEKVFDELKNKDTYDFDISVKQRHL
                     TVKINGVKVISMEITDTIFEGKVGLISFNETMTASDFSVEDIAIPKATAHLTNLQVTG
                     GDLDKKYTQDTNDYAITVPNTVDKLSITPTVSDNGTVTIGEQKAENGKATEVSLKIGS
                     QEIPITVTSTNGAVETTTLHVLRNVDGSAYTVPTRPQYHFSQAMGWSNDPNGMLYYKG
                     EWHLFFQHNQARTTWGELEWGHAVSKDLIHWEELPRPLNYQEDNGAMFSGCGVVDDEN
                     TSGLFGDEKGPGKGGLIALYTQNNELNGHRGQDQCIAYSKDNGRTWTKYKGNPILKWD
                     EDHLKDTAFRDPKVFRHDGKWFMVLAGGILRIYSSDDLIHWNIESTYKGSDDIGNPAG
                     LRVETECPDMYPLTADDGTTKWVISEGGRYYRIGDLKKIDGHWTFVQDKDSDRYVMNF
                     GPHSYAAMTYYIGNGETTNGKPENRRIMINWASTWADGYCNNVDKVTGQWGYNGFFNL
                     QTELNVKKIDGKYKLVQTPIDEYKTLRVNEAATKLEDVTIPKKTENSENLLSGVKAGQ
                     YEVVAELTPQAGTKEVGFKLRTNKSGSQETVVSYNTETKKVSINGEKSGTHPAGQQTK
                     NIVSDAIVTEKDGKVKLDIFVDESSVEVYGQDGQVTGALAVFPSVSSTGMEVYSEGGE
                     TTGNITVYPMKSIWENKITDANTATDLYISTDSGSGEYNVGDKISVNAAISPMKADQK
                     VTWKVSNNKSNKVKVVKTEDDELQLEALKEGSVTVTATTANGLSRSIDIFVSAADDGI
                     SLSDWKQSVRILTVVQEKPVETHS"
     gene            complement(3504..3788)
                     /locus_tag="PLC71_00375"
     CDS             complement(3504..3788)
                     /locus_tag="PLC71_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01134.1"
                     /translation="MTTQTRAQQLKEIEFQTQMLNNLKKWIRNLIILSSIGIILAYWG
                     LGVQSKMPFTVFGVAGVIITIISVILCVVIGLGIKRGRANVDKILQLVKA"
     gene            complement(3804..4640)
                     /locus_tag="PLC71_00380"
     CDS             complement(3804..4640)
                     /locus_tag="PLC71_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HRM01135.1"
                     /translation="MAEKKLSKKALNKSFRNWAYGNLTCFSQEHMQTFGYLCAMLPLV
                     EELYDTKEEQKEAMNTYTAFFNTEPQVGSIIVGITAGLEEAKANGNEDIDAEAINGIR
                     AGLMGPLAGIGDSLVVGTLIPILLGIALGLSTGGSVVGPIFYIVAWNLIMAFGMKFLY
                     FKGYELGGKAVDILVGEQATAIREAIVMLGTMVVGSVAATWVSVKTSFHLVNSAGKEF
                     LNLQNQLDGVYPGFLTAAFVIFCWWLMSKKKVSPVVTMLILVVVAFIGVMIGFFNPGL
                     KY"
     gene            complement(4642..5496)
                     /locus_tag="PLC71_00385"
     CDS             complement(4642..5496)
                     /locus_tag="PLC71_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRM01136.1"
                     /translation="MTISWLQAIILGIFACLSSMPGMGGTTIGNYTLGRPLVAGLVCG
                     IVLGDIQAGVLVGAAMQVVYIALVTPGGTVSADVRAVSYIGIPLAMLALNSYGLDPAS
                     AKGISMATSFGAMVGTIGTVLFYGTATINLVWQHIGWQAVEKGEFKRLYVVDMVLPWI
                     SHIICSFLPAVVMCKLGVPVVEAIKTTLPMDGIAMKTLFTVGSLLPCVGIAILLKQVV
                     TKAVDFIPFFFGFTLAASMGLNLVSVTVVAAMFAIIYYNIKMVGIRAKEAALASGGSF
                     DDDDEEDI"
     gene            complement(5523..6020)
                     /locus_tag="PLC71_00390"
     CDS             complement(5523..6020)
                     /locus_tag="PLC71_00390"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM01137.1"
                     /translation="MSVSFLRIDDRMIHGQTVTRWSLEYPCDGLIAVNDKAATTDALK
                     MAYKSASTKKTFVWTYEAWKKKNQKVLDSKDNYFLITKDPIQMKKILVDDGFVPSDVK
                     KVIVGPCNDRPEAVKLGNNQSITQEEAEALEAISKAGYEIEFALIKEASIGTWDKFRG
                     QFGFN"
     gene            complement(6041..6475)
                     /locus_tag="PLC71_00395"
     CDS             complement(6041..6475)
                     /locus_tag="PLC71_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HRM01138.1"
                     /translation="MKYVVLVSHGTFAPGLHSVLKMLAGGEREDVISVGLEDGMSADE
                     FAEKFSNAISKITEDDEIILLGDIVGGSPLTNAIEQVSNKGLIGQTVIFGGMNLAMAL
                     TATLMKDGVDTDMLKDSLINEAKDAVKEFVMTSANDDEEDDI"
     gene            complement(6637..7956)
                     /locus_tag="PLC71_00400"
     CDS             complement(6637..7956)
                     /locus_tag="PLC71_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRM01139.1"
                     /translation="MRQKLEKWWKRIIAVGLVISLLSVGFVIYPKQQKIVLTFGMFAG
                     NKWDVPDDNCYQMIDQVIKEFEKEYPNVTIKYESGVMKDDYSEWLSQKALKGDLPDVF
                     MVLPEDFSIFSSVGMLKNLDSYTKIDKAFKKSNYYEGSYDAGTYKGTQYALPYESVPT
                     LMLVNKTLLKKEHIKMPGNHWTWDDFYKICQKVTKDTDGDGRLDQFGVYNYKWNDATL
                     SNGGTLFNQSGTKCNLSSEPIENAILFTKKIEKISSFRNLTSDDFDSGNIAFRPMTFS
                     EFRTYKPYPWKINKYFDFKWDCIRLPSGPDGENKTQVDNLLMGISNQTKQGDMAWQFL
                     KKLCYETKTQQKIFQYRQGISPLKAVTNSKQAQQVLEKSMGENMTDKMKLLDELMNNA
                     LQIPKFRRYNEAYQKIDSEMTRILTDEEEFDSNILKLKQEIDKFLNQ"
     gene            complement(7957..8625)
                     /locus_tag="PLC71_00405"
     CDS             complement(7957..8625)
                     /locus_tag="PLC71_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM01140.1"
                     /translation="MIKVMIADDQELIRQSLQIVLEMKEGIEVTATAKDGREVIQEVR
                     KDKPDVILMDVRMPEMDGVQCTQIIKEQYPDVKIIILTTFDDDEYVYNALKHGASGYL
                     LKGISMDELEKAIKTVHSGGAMINPEVATKVVDFFSEMAQSNYDTSIHAQNVEEITDA
                     EWEIIKLVGRGMSNKEISTELSLSEGTVRNYLSTILSKLDLRDRTQLAIWEVQSGSAK
                     YGRR"
     gene            complement(8622..9944)
                     /locus_tag="PLC71_00410"
     CDS             complement(8622..9944)
                     /locus_tag="PLC71_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM01141.1"
                     /translation="MDRKNQNLYYIKILMGVINFIILSFLSAVFILTLNKINCDFGAR
                     NFLENITYLPNEPVTSTVLIFIAFFLLLGIIEIRRKQRKRQQVSVILYAAEILLCLII
                     IKFVYVNYNGIILLVIADIVTDIKDKHTRGIFFVIMGMIYLFSDYDIISMFIDMISFQ
                     QFLSVYPAKVSMMIQGTRNILVALNAIAFIAYMIILMRNQMKENARIGLLNLQLQTAN
                     IRLKEMNEQLKDYSEMTEKMAETRERNRIAREIHDTLGHTMTGLSAGIDACIAMIDFS
                     VDATKEQLNKISQVARQGIKDIRRSVNKLRPDALEHSGLQEALEKMINETMQVSDVKI
                     NYDCQVEVLKFNQDEEDMIYRVVQESITNAIRHGKAKQIDVNLWKEDKWLNLEIKDNG
                     IGCEEIHTGFGLIHIEERIKMLKGTVEYDGSNGFKVTARIPIRWGEKL"
     gene            complement(9957..10937)
                     /locus_tag="PLC71_00415"
     CDS             complement(9957..10937)
                     /locus_tag="PLC71_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRM01142.1"
                     /translation="MNQQSKKNLTNRIVALAGITVFAFFIGIYVCVFSNYLKKYNTRK
                     KLFGATYMTMNNEFYKIVNNQIRMQVEKNGNQMITLDPALDQKKQNEQIKQLVKRKVD
                     AIFLNPVDWKAVEPGLVAAKKAKIPVIVIDSQVYRSDLVAMTIVSDNYKAGVLCAKEM
                     MKQKDQANIILLTHNAAKSAVDRINGFLDTIKNNKNYKILASADTQGQIERALPKVDK
                     IIEEYQNIDIVMGLNDPAAMGALAALDSKGAREGVLVYGIDGSPEGKKLIKEGMMTGT
                     AAQSPKQMADTAVNAAYQILQGEKVEKKKVISVQMITKKNVEQTDISRWQ"
     gene            complement(11038..12936)
                     /locus_tag="PLC71_00420"
     CDS             complement(11038..12936)
                     /locus_tag="PLC71_00420"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRM01143.1"
                     /translation="MKDGFIKVAAATPKIKVADPAYNTEEILKIIDETEKNGASILVF
                     SELTISGYTCGDLFLQQPLLTECKNQLLRIVKATENKSMLVVVGCPIVIKQKLYNCAV
                     VISDGSILGIVPKTHLPNYSEFYELRHFTSGEGLEEDLWFGEEFGYVNVAVNQLFKCK
                     EIPELVVACEICEDLWVPLPPSTYHAMAGATVICNPSASVETTTKESYRRSLVSNQSA
                     RLLAAYIYADAGEGESTQDVVYSGHHLICENGSVLAEAKRFTNEIIYADIDVQKLAAE
                     RRKMTSFPGGQTDDYFEQEFSLEVKENKITRTFPKAPFVPDNQDERDKRCDEILSLQS
                     MGLKKRLEHTNCKHAVVGISGGLDSTLAVLVTARAFDLLDIPRENLICVTMPCFGTTD
                     RTYQNAVSLIKELGATLKEVRIEKAVRQHFADIGHDENNHDVTYENSQARERTQILMD
                     MANQYNGMVIGTGDMSELALGWATYNGDHMSMYAVNCSVPKTLVRYLVLYYAETTENK
                     KLSEVLMDVLDTPVSPELLPPVDGVISQKTEDLVGPYELHDFFLYYMLRFGFPKAKLY
                     RMAKLTFDGVYDDETIKKWLDKFYWRFFSQQFKRSCLPDGPKVGRVAVSPRGDLRMPS
                     DASPTAWL"
     gene            complement(13007..14080)
                     /gene="prfA"
                     /locus_tag="PLC71_00425"
     CDS             complement(13007..14080)
                     /gene="prfA"
                     /locus_tag="PLC71_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRM01144.1"
                     /translation="MFDKLEGLVDRLDTVLQELNDPDVAGNQNRFRDLMKEQNELTPI
                     VEKYKEYKAEKQNIEDSLMLLEEESDEEMKELAKEELNESKANVERLEEELKILLIPK
                     DPMDDKNIIVEMRAGAGGDEAGLFVADVARMYRNYAESKRWKVNTLSFNESGIGGFKE
                     LVFMVSGQGAYSRFKYESGVHRVQRIPATESGGRIHTSTITVAIMPEVEDVEVEIDMN
                     DCKFDVFRASGNGGQCVNTTDSAVRLTHIPTGIVVSCQDEKSQLKNKDKALKVLRSRL
                     YELEQQKAHDEEAAKRKSQIGTGDRSEKIRTYNYPQGRVTDHRIKLTLHKLDSIMNGD
                     LDELIDSLTAADQAAKLSNMEEE"
     gene            complement(14082..14933)
                     /gene="prmC"
                     /locus_tag="PLC71_00430"
     CDS             complement(14082..14933)
                     /gene="prmC"
                     /locus_tag="PLC71_00430"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRM01145.1"
                     /translation="MNRREWVLYGQKELEEAQIENASGDAWYLFSECFHISREDYLFG
                     MTDEINDKEAEERYKELIQKRKEHVPLQYILGTQEFMGYTFKVTPDVLIPRADTETVL
                     EEVLDQLKQSKKPDTILDICTGSGCIAISLALILNPEVCIGTDISEKALKIAKANGEN
                     LAPMVKFIQSDLFENVTGSYDLIISNPPYITTEECGKLMPEVKDYEPMLALDGKEDGL
                     YFYKKIIKEAKNYLNPQGMLAFEIGYDQGEAVKNLMEAQDFACVEIKKDLAGLDRLVF
                     GFAREGE"
     gene            complement(14926..15873)
                     /locus_tag="PLC71_00435"
     CDS             complement(14926..15873)
                     /locus_tag="PLC71_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HRM01146.1"
                     /translation="MAVRIGGQAVIEGVMMKNMDRYAVSVRKPNGKIETKVEECVSFA
                     EKHPLFQLPVFRGMANFLESMVIGMKTLNYSASFYEDEEEQTESRTEQLLEKILGEKA
                     EKIIMGIVLVFSLAISIGLFMILPYIASEALGKLIRNEYVILFMEGIIRIAIFLGYIV
                     LISRMEDIKRVFMYHGAEHKTINCLEAGVPLTPENVDNFSRLHKRCGTSFIFIVMIIS
                     MVFFFFIRVDTIWLRIVLRLLFLPLVAGVSYEFIRLAGSSDHPLVQIFSKPGLALQKL
                     TTKEPDHSMIEVAIASVEGVFDWREYLENLHKGEQKEDE"
     gene            complement(15962..16162)
                     /gene="rpmE"
                     /locus_tag="PLC71_00440"
     CDS             complement(15962..16162)
                     /gene="rpmE"
                     /locus_tag="PLC71_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRM01147.1"
                     /translation="MREGIHPNYYQAKVVCNCGNEFVTGSTKEDIHVEICSKCHSFYT
                     GQQKATQARGRIDKFNRKYGVK"
     gene            complement(16293..>16518)
                     /locus_tag="PLC71_00445"
     CDS             complement(16293..>16518)
                     /locus_tag="PLC71_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRM01148.1"
                     /translation="AEKRIFPAVDIAKSGTRREELLLTKDEREVVTALHKELSGNRSE
                     EFMEQMLDLFKRTQNNKEFVALTKRSLLRK"
     assembly_gap    16519..16528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16529..17657)
                     /gene="rho"
                     /locus_tag="PLC71_00450"
     CDS             complement(<16529..17657)
                     /gene="rho"
                     /locus_tag="PLC71_00450"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRM01149.1"
                     /translation="MTYEKKSVIELREIAKERKIKGISTLKKQELIKVLENYDQEQSK
                     QKEEPQIQREPKQSYSEHQERKHHQKEFPKNIKSLDSGEKAHGILEVMPDGYGFIRCE
                     NYLPGENDIYVSPAQIRKFNLKTGDILTGAIRIKTQSEKFSALLFVESVNGYKPEMAA
                     RRQHFEDLTPIFPNERLGMETERSSIPMRMVDLLSPVGKGQRGMIVSPPKTGKTTLLK
                     QMAQSISQNYKDIKLIVLLIDERPEEVTDFKESIEGKNVEVIYSTFDELPEHHKRVSE
                     MVLERAKRLVEHKEDVVILLDSITRLARAYNLTVPPSGRTLTGGLDPAALYMPKKFFG
                     AARNMREGGSLTILATALVETGSKMDDVIFEEFKGTGNMELV"
     gene            complement(17748..18473)
                     /locus_tag="PLC71_00455"
     CDS             complement(17748..18473)
                     /locus_tag="PLC71_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigF/SigG family RNA polymerase sporulation
                     sigma factor"
                     /protein_id="HRM01150.1"
                     /translation="MDKTRELLRLTKEGNEAAREQIILNNVGLIWSVVRRFTNRGYES
                     EDLFQIGSIGLMKAVDKFDLSYDVKFSTYAVPMITGEIRRFLRDDGMIKVSRSLKENV
                     YKIMKAREKFCQQYQREATIEEISEITKITREEIVTSLEASNEVESIHQSAYQKDGSE
                     ISLLDKLSKGENESEKVVDHVLLKEAMKHLEQKEQQMIYYRYFEGKTQQEIAKIFHIS
                     QVQVSRIQKKILKKLRRQMIGNI"
     gene            complement(18466..18894)
                     /gene="spoIIAB"
                     /locus_tag="PLC71_00460"
     CDS             complement(18466..18894)
                     /gene="spoIIAB"
                     /locus_tag="PLC71_00460"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HRM01151.1"
                     /translation="MLHLEFNSDSENEGIARIVTAAYIMKFDPDMEEMDDIKTAVSEA
                     VTNCIVHAYHNPDGKISMDLSDQNGILRIEIKDQGIGIPDVKKAMEPMFTTKPEEERT
                     GMGFSFMEAFMDQVEVISKQGEGTTVIMTKKIGDQDKLNG"
     gene            complement(18914..19219)
                     /locus_tag="PLC71_00465"
     CDS             complement(18914..19219)
                     /locus_tag="PLC71_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390562.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HRM01152.1"
                     /translation="MATEYKIENRVLYIYLDGDLDHHLAKKVKHKCDIILKSYPIKDI
                     EFDFKNSGFIDSSGIGVILGRYRQVQAMGGTVKVSNMSASVRKIIHMAGLHQLIEEI"
     gene            complement(19377..20576)
                     /locus_tag="PLC71_00470"
     CDS             complement(19377..20576)
                     /locus_tag="PLC71_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRM01153.1"
                     /translation="MLDFNEELAKFQPCADLEEAEENIYGKELEEIKKNKTELYKANQ
                     MIKKYNQALNYAKQGNDDLAMLQLKNVVAAIPNFVDAYLLMALLSIKGENYDNARTFL
                     DTILKIDPNNESAVEYGKEFETKVVEEEPQTTEPEKKDKKKKEKKKVVSQPEEPKKKK
                     NPFNISSIQENEAGKSPMFYMVTGIVIGVIVAAVLIYPTVRASFSHKNSTQVEDYKEQ
                     ILAKDTQLKASEKKVKEAKAAQKKAEDELDEYIGTSKKDGVYDLLLSALQKYSDRDYT
                     GSADALLDIDSKKLTTKNMKAIYKDLTAKVYPSAGTGLYSKGKNAYWAKDYKTAISYL
                     TKAIKVSDTNRYAYDYLAKAYEGASDQKNAKKTYQTIIEKFPGTSQAINAKSRIDAMG
                     KKSAKSL"
     gene            complement(20563..21828)
                     /locus_tag="PLC71_00475"
     CDS             complement(20563..21828)
                     /locus_tag="PLC71_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRM01154.1"
                     /translation="MLTEEMTMTYEESVKYLYKIAGFATKSSLEDIKYFLERLGNPQK
                     ELHMIHVAGTNGKGSVCAFLEALLTQHGKKTASFTSPHVVKINERIKLCAKEISDERF
                     ASAAVKVRHAIETGITEGKNSPSFFEAVFLMAMVIFQEEQPDICMIETGMGGRYDATN
                     VICPKVSVITSISEDHMEFLGQTLEEIATHKAGIIKPGIPVVTIEQEPKVKEILSEEA
                     RQKKSRIYDISEDNLNFKEKAGKYIDFLNANAYDKERDVRTNIAGEFQKSNLAAALQT
                     AELICGRLDEDKIYEALSQIRISGRMEEIAPGIIVDVSHNIQGMQGFVQTVEANYHGM
                     KKRILFAASHQNEEEYMKNILKTIPEIEAFYTVGIHKRRIDEAEFQDAFRKMIDRNQE
                     TTVCFVVGSFYLAGMAKEFINQEEENVRL"
     gene            complement(21822..22616)
                     /locus_tag="PLC71_00480"
     CDS             complement(21822..22616)
                     /locus_tag="PLC71_00480"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM01155.1"
                     /translation="MIKLIATDIDGTLVADGTLDLNPEYYDVIKELKRRGTIVLAASG
                     RQRASIEKVFTPVLDDISFISENGTCIHSKDYQYVDVIDPEIVRTYIEEARQFPGCEI
                     AINKDNMTYMENIGIYQHLVGDYGYRGDLVDDVLGNPEGVCKMSIFHHNCAEDVVGDE
                     FIKRWGKKMNVVVSGKCWVDCANKGANKGSALRHFQEEYGITPDETLAFGDNLNDIEM
                     LKRASHSFAVENARDEVKEAANFVAPSYKEDGVLQVLKAVLSGELC"
     gene            complement(22698..23615)
                     /gene="metA"
                     /locus_tag="PLC71_00485"
     CDS             complement(22698..23615)
                     /gene="metA"
                     /locus_tag="PLC71_00485"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HRM01156.1"
                     /translation="MPIRIDNDLPAKAALEEENVFVMDKERACTQDIRPLKIAILNLM
                     PNKLDTELHLLRSLSNTPLQLDITFLQTESYVPTHVSESHMEKFYNYFSEIKDKRFDG
                     LIITGAPVELKEFEEVDYWDEVVEIMEWSKTHVTSTLYICWAAQAGLYYHYGIKKHIL
                     NKKISGVYEHHPLHNKLPIIRGFDDKFYVPHSRNTGVDGEAIRKNKELTIVAESDETG
                     PYIILNSTGSQVFVTGHPEYDVMSLHYEYVRDVKRGLNPDIPKNYYKDDDPTKKPVKS
                     WRCHANAMYYNWLNYYVYQVTPYDLEKDK"
     gene            complement(23724..25679)
                     /gene="ligA"
                     /locus_tag="PLC71_00490"
     CDS             complement(23724..25679)
                     /gene="ligA"
                     /locus_tag="PLC71_00490"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRM01157.1"
                     /translation="MNDLDRMKELIHTLNEAGKAYYQQNKEVMSNLEYDKMYDELLEL
                     EKKTNTVLSNSPTIHVGYELMTALEKEAHPSPMLSLDKTKEPDQLAAWLNGQEGILSW
                     KLDGLTIVLTYEHGELIKAVTRGNGEVGEVVTNNARVFENVPRRIAYQGTLVIRGEAI
                     IPYSDFYKMNEEIEDVDSQYKNPRNLCSGSVRQLNNEITAKRHVHFYAFSVSDVEGMD
                     FDNSFEKKLDWAASLGFDVVEHIRVTEDTIRGEIEKFSKKIEQFDLPSDGLVLVYDDL
                     AYGKSLGRTAKFPRDSIAFKWADETAETTLTEIEWSPSRTGLINPVAIFEPVELEGTT
                     VSRASLHNISVMEELQLGIGDEIVVYKANMIIPQLAENKTKSGNIEIPHTCPACGGET
                     KIEDENGIRTLVCTNEFCSAKKIKSFSHFVSRDAMNVDGLSEATLQKMIDVGLLNEIY
                     DIFTLKDHKEEILELEGFGEKSYQNLINAINDSKQPALANFIYSLGIPNVGLSNAKLI
                     CKHFKEDFNAIREADAEDFIAIDQIGPMIAEAMVSYFHTPHNQKILEQLLQYVQFEKK
                     EESKTEQILEGKTFVVTGSLDHFDNRKQLKEEIEQMGGKVTGSVSKKTDYLINNDKMS
                     QSSKNKKAMELNIPILSEEDFLNLIGR"
     gene            complement(25672..26406)
                     /locus_tag="PLC71_00495"
     CDS             complement(25672..26406)
                     /locus_tag="PLC71_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRM01158.1"
                     /translation="MQETKRKTEDAVRINKYLSQAGICSRRQADTYVEEGRVEVDGEV
                     AVSGTKVLPGQSVTFDGKPVKIQEDLIFLAFHKPRGIVCTASKEEKDNIIDYINYPTR
                     IYPVGRLDKDSQGLILLTNDGEAANQIMKARNYHEKEYEVTVNKRITNEFIHDMRNPV
                     PLDEINAVTRRCKVIKEGPNEFRIILTQGLNRQIRRMCEHFGYRVTKLKRVRVMNIKL
                     GDLKEGRYRKLTPDELRELKKELKSE"
     gene            complement(26409..27998)
                     /locus_tag="PLC71_00500"
     CDS             complement(26409..27998)
                     /locus_tag="PLC71_00500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM01159.1"
                     /translation="MKLMFIGADHEVTGSCHYVEACGKRFIVDYGMEQGLNVYENVDL
                     PVNAGNLDFILLTHAHIDHSGLIPLLYAKGFRGKIYATRATTDLCEIMLQDSAHIQEF
                     EAEWKNRKARRSGKPEVVPIYTMDDAINVMQYFVPCPYGRQIEVADGITIKFTDIGHL
                     LGSSSIEVWLREADVEKKLVFSGDIGNFNQPLIKDPSYTREADYVIMESTYGDRYHGK
                     HQDYVTELTNVIQRTFDRGGNVVIPSFAVGRTQELLYYIRQIKAEDRIKNHGDFKVVV
                     DSPLAVEATKIFNMNIDETYDEEAMELVKQGINPITFSNLHLSITTEESKEINFETEP
                     MVIISASGMCEAGRIRHHLKHNLWRSENTIIFVGYQAYGTLGRSLIEGAKEVKLFGEA
                     IKVAADIVQLEGLSGHADKNGLLKWVNSFEKKPEEVFVVHGEDAVCEAFTSCLKDEYK
                     FKARAPYSGDVFDLIAGEWTEWGSRERIQKKPKQSSAYIRLLTASERLKSVIEHNEGG
                     TNKDLGKFADQIIALCDKWER"
     gene            complement(28002..29192)
                     /locus_tag="PLC71_00505"
     CDS             complement(28002..29192)
                     /locus_tag="PLC71_00505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM01160.1"
                     /translation="MKQNKNKGNSMEIVYQKNKDKQPVYDMYQKIFEDPEPFAQYYFE
                     EIYATNQVILAEEDNKILGMLHLNPYHIRAGKKTYTLNYIVAVAVWKEYRRRGIMAEM
                     LKKCFNDMHEQQQPFTYLMPANKAYYEPFQFRFVMDWEETMIDDHNISYTDDTTDRNH
                     KIVHIMAEDYQTIQDFLEWFMEQYKIYTVPDEPYLRRLEKESQSGDGSLLAYYEDDLL
                     QGVFAESFEEDEVYIRWAYSLEPEHMINQIKQRHKGKKIYITEGNLFKGNSIGKDFIQ
                     SKKVPKIMARITNLTAWGEILQGKNDFTFRILVKDPYIKDQNGVFQFQCLNHKISIQR
                     ADIPQDETLDIHTTDAQGWEDEISIDELTQVFFDYDAGQILKEHEYLKDIVPAGPIYI
                     SEEV"
     gene            complement(29179..29655)
                     /gene="ispF"
                     /locus_tag="PLC71_00510"
     CDS             complement(29179..29655)
                     /gene="ispF"
                     /locus_tag="PLC71_00510"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRM01161.1"
                     /translation="MRVGMGYDVHQLVEGRDLILGGVNIPYEKGLLGHSDADVLLHAI
                     TDAILGAAALGDIGKHFPDTDPAYHNADSMKLLAEVVRLIEEKGYEIGNVDATVIAQR
                     PKLLNYIPTMKENISKTLKVDPDQVNVKATTEEHLGFTGEGLGISAQAVCLLNETK"
BASE COUNT         7413 a         6684 c         4659 g        10975 t
ORIGIN      
        1 aacctgagtt tcaatctgtg ttgttccagc tttctttgct gtgatgttac catcttcatc
       61 tacatctgcg attgttttgt ccaaagattt gtatttgatg atgttgaaac tgttgtcctc
      121 gatattggaa acatcaattg ctgctttctc gcctgctgtt aatgcaagtt tgtcagaaac
      181 tgttggaact ttcacttcta atggtgtgac tttcatattc tggaatgtta cttttccatt
      241 gaatacatta aatccaacat atcttccttc tttgtaatta ttctcaactt ttacatctct
      301 tacattcagt accagcttgc cctcaatata cactttatac tggttacctt ttactacaac
      361 tttgaaatga tatttctgtc cttcgcttaa gttgtaattt ccatcttttc cgatgtctcc
      421 ggttccgcct ccaaatgtaa agattcttgc tgtactacct tttctttgtg tgtctacatt
      481 agctgcatat gcactagtag aatctgggtt ctgtccacgg aataataatg caaatgcctg
      541 tccactgtga taaacaacat ctgattcaaa aatataatca tctgaactga tctttgttcc
      601 tgacattaag aatgcgtctc cactggcttc tcctgtacaa ctgttagaat tctcactgat
      661 tgtttccagt cactaagtga aataccatcg tctgctgctg aaacaaatat atcaatactt
      721 cttgataatc catttgctgt tgttgctgta acagtgacac ttccttcttt tagtgcttct
      781 aactgaagtt catcatcttc tgttttaaca actttaactt tattactttt gttattactt
      841 actttccatg ttactttctg atctgctttc attggagaga ttgcagcatt tacactgatc
      901 ttatctccga cattatattc tccacttcca gagtctgtac tgatatatag atctgttgct
      961 gtgtttgcat ctgtgatctt attttcccag atactcttca ttggataaac tgtgatattt
     1021 cctgttgttt ctccaccttc agaatatact tccattcctg tactactgac agatgggaat
     1081 actgctaatg ctcctgtaac ctgtccatct tgtccatata cttcaacact tgattcatca
     1141 acaaagatat ctaattttac ttttccatct ttctccgtga cgatcgcatc ggatactatg
     1201 ttctttgtct gctgtcccgc tggatgtgta ccagattttt ctccattgat ggatactttc
     1261 tttgtttctg tattataaga tacaactgtt tcctgacttc cagatttgtt tgttcttaat
     1321 ttgaatccaa cttcttttgt tcctgcctgt ggtgtaagtt ctgcgacaac ttcatattgt
     1381 cctgctttga ctcctgataa cagattttcg ctgttttctg tcttctttgg aatcgtaaca
     1441 tcttcaagtt tggttgctgc ttcgttgaca cgtaacgtct tatattcgtc aattggtgtc
     1501 tgtaccagtt tatatttacc atcaatcttc ttcacattta attctgtctg aagattaaag
     1561 aatccattgt atccccactg tccagtcact ttatcaacgt tattgcagta tccatcagcc
     1621 catgtacttg cccagttgat catgattctt cggttttctg gttttccatt ggttgtttcc
     1681 ccattaccaa tataatatgt cattgctgca taagaatgag gtccaaagtt catgacataa
     1741 cggtcgctgt ctttatcctg tacaaatgtc caatgtccat caatcttctt gagatcaccg
     1801 atgcgataat aacgtcctcc ttcactgata acccattttg ttgttccatc atctgctgtt
     1861 aaaggataca tatcaggaca ttctgtttca actcttaatc ctgctggatt accgatatca
     1921 tcacttcctt tatatgtact ttcaatattc caatgaatca gatcatcaga agaataaatt
     1981 cttaagattc ctcccgctaa taccataaac cattttccat cgtggcggaa tacttttgga
     2041 tcacggaatg ctgtatcttt tagatgatct tcatcccatt ttaagattgg atttccttta
     2101 tattttgtcc atgttcttcc gttatctttg ctatatgcga tgcactgatc ctgtcctctg
     2161 tggccattta attcattgtt ctgtgtatag agtgcgataa gtccaccttt tccaggtcct
     2221 ttttcatctc caaataaacc ggatgtattc tcatcatcta caacaccaca tccagagaac
     2281 attgctccat tatcttcctg ataatttaat ggtcttggca attcttccca gtggatcaga
     2341 tctttagaaa ctgcatgtcc ccattctaac tctccccatg tcgttcttgc ctgattatgc
     2401 tggaagaata aatgccattc tcccttataa taaagcattc cgtttgggtc attgctccat
     2461 cccattgcct gtgaaaaatg atattgtggt ctggttggaa ctgtatatgc tgaaccatct
     2521 acatttctta atacatgtaa tgttgtagtt tctactgcac catttgtact tgtcactgtg
     2581 ataggaattt cctgagatcc aatctttaat gatacttctg ttgcttttcc attttctgct
     2641 ttttgttcac cgatcgtcac tgttccatta tcacttacag ttggtgtgat agataattta
     2701 tcaactgtat taggcactgt gatcgcataa tcatttgtat cctgtgtata tttcttatct
     2761 aagtcgccac ctgttacctg taaatttgtt aaatgtgctg ttgcttttgg aatcgcgata
     2821 tcttctacag aaaaatcaga tgctgtcatt gtttcattga aggaaataag accaaccttt
     2881 ccttcaaaaa tcgtatctgt gatctccata ctgatgactt ttacaccatt gatcttaact
     2941 gtcaaatgac gttgttttac agaaatgtca aaatcataag tatctttatt ttttaattca
     3001 tcaaatactt tttctttctg aagatcgttg ttacgtcctc cttggaaaca gatcaaacgt
     3061 acagctccag tagatggagt aacattggct actacagaac ctttctttgg atcttttgct
     3121 tcctttgctc ccataatcac accgacagct ccgtcttctt ctccttgtgt cagttttgct
     3181 ttgaccgcat aattatcttc ttttttgatg tttgtgatac cgatattatc accagctcct
     3241 gataactgga tcttatcatt gtctactttt ccattccctg taaatgtcca atctatcgcc
     3301 tgatgctgta attgtgctgc atttacagtt acatgattgt atgctattac tggcgcgata
     3361 gaagtgacac tgactacact tgccatcatt attgctacta atcttgggct aactttcctc
     3421 atattttttc cctttctttg tagaaaaaaa ggacgatcta actataatcg tcctttttca
     3481 atcaaaaaac aatagttcta tgcttaggct ttcactaatt gtaatatctt gtctacgttt
     3541 gctcttcctc gcttaatacc aaggcctatg acaacacaaa gtatcacact gatgatcgta
     3601 atgatcactc cagctacacc aaatactgta aatggcattt tactttggac acctagtccc
     3661 caatacgcaa gtatgattcc gatagaagat agaataatga gattgcgaat ccattttttg
     3721 aggttattga gcatttgtgt ctgaaattca atttctttta gctgctgtgc tcttgtttgt
     3781 gttgtcataa tatacttcct ttcttagtat tttaatcctg ggttaaagaa tccgatcata
     3841 actccgataa atgctacaac tactaagatc aacattgtaa caactggaga tacttttttc
     3901 tttgacatta accaccaaca gaagattaca aaagctgctg ttaagaatcc tggatatact
     3961 ccgtctaact gattctgaag atttaagaat tcttttcctg cactgtttac aagatggaat
     4021 gatgttttta ctgataccca tgttgctgct acagatccaa caaccattgt tcctaacata
     4081 acgattgctt cacggatcgc ggttgcttgc tcaccaacca ggatatctac ggcttttcca
     4141 cccagttcat atcctttgaa gtataagaac ttcattccaa atgccatgat caagttccat
     4201 gctacgatat agaagatcgg tcctacgaca gatccacctg ttgataatcc cagtgcgata
     4261 ccaagtaaga taggaattaa tgtacctaca actaaggaat caccaatacc tgctaaaggt
     4321 cccataagac cggcacggat accattgatt gcttctgcat cgatatcttc atttccattt
     4381 gcttttgctt cctcaagacc agctgtgata cctacgatga tagatccaac ctgtggttct
     4441 gtattaaaga aagctgtgta tgtattcatt gcttctttct gttcttcttt tgtatcatat
     4501 aactcttcta ccaatggaag cattgcacac agataaccaa atgtctgcat atgttcctga
     4561 gagaaacatg ttaagtttcc ataagcccag ttacggaatg atttatttaa cgctttttta
     4621 gatagttttt tctctgccat attaaatatc ctcctcgtca tcatcgtcaa aggaaccgcc
     4681 agaagctaag gctgcttctt ttgctctgat tcctaccatt ttaatgttgt agtaaataat
     4741 tgcgaacatt gctgctacaa ctgttactga tacaaggttt aatcccatac ttgctgctaa
     4801 tgtaaatccg aagaagaatg gtataaagtc aactgctttt gttactacct gttttaataa
     4861 gatcgcaata cctacgcaag gaagtaagct acctactgta aacagtgttt tcattgcaat
     4921 tccatccatt ggtaatgttg ttttgatcgc ttctacaact ggaactccta atttacacat
     4981 tacaactgct ggtaagaagc tgcagatgat atgagaaatc catggaagaa ccatatctac
     5041 aacgtataat ctcttgaatt cacctttttc aacggcctgc catccaatgt gctgccatac
     5101 aaggttgatc gttgctgttc catagaataa tactgtaccg atcgttccta ccattgctcc
     5161 aaatgatgtt gccatggaga taccttttgc tgatgcagga tctaatccgt atgaatttaa
     5221 tgctaacatt gccagtggaa taccgatata agatacggca cgaacgtctg ctgatacagt
     5281 tcctcctggt gtaaccaatg cgatatatac aacctgcatt gccgcaccga ctaaaacacc
     5341 tgcctgaata tctccaagta cgataccaca gacaagacct gcaactaatg gtcttcctaa
     5401 tgtgtagtta ccaatggttg tacctcccat accaggcata ctggataagc aggcaaaaat
     5461 acctaaaatg attgcttgta accaactaat tgtcataata gtttctcctc tctattggtt
     5521 ttttagttaa atccaaactg acctctgaat ttgtcccatg ttccgatgga cgcttctttg
     5581 atcagtgcga attcgatttc ataaccagct ttgctgatcg cttctaaagc ttctgcttct
     5641 tcctgtgtaa tagattggtt attaccaagt ttgacagctt ccggtctgtc attacatggt
     5701 cctacgatta ctttctttac atcacttgga acaaatccat catccactaa gatcttcttc
     5761 atctggattg gatcttttgt gatcaggaag taattgtctt tgctgtctaa tactttctgg
     5821 tttttctttt tccatgcttc atatgtccaa acaaatgttt tctttgtgct ggcactcttg
     5881 tatgccatct taagtgcatc tgttgttgct gctttgtcgt tcactgcgat cagcccatcg
     5941 catggatact ctagtgacca acgtgttact gtctgaccat ggatcatacg gtcatcaatt
     6001 cttaaaaatg aaactgacat atttttttcc tccttaaaat ttaaatatcg tcttcttcat
     6061 catcgtttgc tgatgtcata acaaattctt taactgcatc ttttgcctca ttgatcaagc
     6121 tgtcttttag catgtctgtg tctacgccgt ctttcattaa tgttgctgtc aatgccattg
     6181 ccaggttcat tccaccaaag atcactgtct gaccgatcag tcctttattt gatacctgtt
     6241 caattgcatt tgtaagaggt gatccaccca caatgtcacc aagaagaatg atctcatcgt
     6301 cttctgtgat cttgctgatc gcattgctga atttctctgc gaattcatca gcactcattc
     6361 cgtcctctaa tccgacgctg atcacgtctt ctctttctcc accagctaac atcttaagta
     6421 cactgtgaag tcctggtgca aatgttccgt ggctgactaa tactacgtat ttcatcctct
     6481 tctcctttct tattcctatc tcttatttac aaacttattc taccaagcat gtaaaaaaag
     6541 gacatctgta aattgtcatg actttcaatt tacaaatgtc cttttttatg tgacaaatgt
     6601 catattcgtg ttttatttgt tttgattttc ttattgttac tgatttaaaa acttatcaat
     6661 ctcttgtttt agtttcaaga tattggaatc aaattcttct tcatctgtaa gaattcttgt
     6721 catctcactg tcaatctttt ggtaggcttc attgtatctt cggaattttg gaatctgcaa
     6781 ggcattgttc atgagttcat ctaacaattt cattttatct gtcatattct cacccatgct
     6841 tttttctaat acctgctgtg cttgttttga attcgtcaca gcttttaatg gtgagattcc
     6901 ctgacgatac tgaaagatct tttgctgtgt ctttgtttca taacataatt tctttaaaaa
     6961 ctgccatgcc atatctcctt gcttggtctg attgctgatt cccatcagca gattatctac
     7021 ctgtgtctta ttctctccat ctggtccgga agggagtctg atacaatccc atttgaaatc
     7081 aaagtattta ttgatcttcc atggatatgg tttatacgtt ctaaattctg aaaatgtcat
     7141 aggacgaaat gcaatatttc cagaatcaaa atcatcagat gtcagatttc gaaaagatga
     7201 tattttttca atcttctttg taaagaggat tgcattttca attggttcgg atgacaagtt
     7261 gcattttgtt ccactttgat taaacaatgt tccgccattg ctcagagttg catcattcca
     7321 tttataatta tatacaccaa attggtctaa acggccatct ccatcggtat ctttggttac
     7381 tttttgacag atcttataaa aatcatccca agtccagtga ttccctggca ttttgatatg
     7441 ctcttttttt agtagtgtct tattgactag catcaaagtt ggtacacttt cgtatggaag
     7501 agcatattgc gttcccttat aggttccagc atcataactt ccttcataat agttgctttt
     7561 tttgaacgct ttatcaatct ttgtatagga gtctaagttc tttaacattc ctacggatga
     7621 aaatatgcta aaatcttctg gcaagaccat aaaaacatct ggcagatctc cttttagtgc
     7681 cttttgtgac agccattctg aataatcatc tttcatcaca ccactttcgt atttgatcgt
     7741 gacattcgga tattcttttt caaattcttt gattacttga tcgatcatct gatagcaatt
     7801 atcatctgga acatcccact tgtttcctgc aaacattcca aaggttaaaa caattttttg
     7861 ttgtttggga tagatcacaa aaccaacaga taataaactt atgaccaatc caacagcaat
     7921 gattcgtttc caccattttt ctagtttctg tctcatctat cttctcccgt atttggctga
     7981 acctgactgt acttcccaga ttgcaagctg ggttctgtct cttaggtcta gtttacttaa
     8041 gatcgtgctg agataatttc gtacagttcc ttcggaaagg cttagttccg tcgagatttc
     8101 tttatttgac atgcctcttc cgactaattt aatgatctcc cattctgcat ctgtgatctc
     8161 ttctacattc tgtgcatgaa ttgatgtatc ataattgctt tgtgccattt cagagaagaa
     8221 atcgaccacc tttgtcgcta cctctggatt gatcatggct ccgccactat gaactgtctt
     8281 gattgctttt tctaattcgt ccatagagat tccttttaac agataaccgc ttgctccatg
     8341 ttttaatgca ttgtatacat actcatcatc atcgaacgtt gtcaaaataa tgatcttgac
     8401 atctggatat tgttctttga tgatctgtgt acattgcact ccatccattt ctggcatacg
     8461 cacatccatt aaaataacat ctggcttgtc tttcctgact tcctgaatga cttctcttcc
     8521 gtccttggca gttgcagtaa cttcgattcc ttctttcatc tcaagtacga tctgtaaact
     8581 ttgtctgatc agttcctgat catctgctat cattacttta atcatagttt ttcaccccat
     8641 ctgatcggta ttcttgctgt gactttaaat ccattacttc catcatattc tacagttcct
     8701 tttaacatct tgatcctctc ttcgatatgg atcaatccaa atcctgtatg aatctcttca
     8761 catccgattc cattgtcttt gatctctaga ttgagccact tatcttcctt ccataagttt
     8821 acatcaatct gcttcgcttt tccatgcctg atcgcatttg taatactttc ctgtactaca
     8881 cgatagatca tatcttcttc atcttgattg aatttcaaga cctctacctg acaatcataa
     8941 ttaatcttaa catctgatac ctgcatcgtc tcattgatca tcttttctaa agcttcttgc
     9001 aatccactat gctctaaggc atctggtctt aatttattga ccgatcttcg aatatcttta
     9061 attccttgcc ttgcaacctg tgatatttta tttaactgtt ctttcgttgc atctacagaa
     9121 aaatcgatca tcgcgataca tgcgtcaatt cctgcagaca gtccagtcat cgtatgcccc
     9181 agtgtgtcat gaatctctct ggcaattcga tttcgttccc ttgtctctgc catcttctct
     9241 gtcatctctg aatagtcctt cagctgttca ttcatttctt ttaaacgtat attggctgtc
     9301 tgaagctgaa ggtttaacaa tccaatcctt gcattctctt tcatctgatt tctcatcaaa
     9361 atgatcatgt atgcaataaa tgcgatcgca ttcaatgcaa ctagtatatt tcttgttcct
     9421 tggatcatca tactgacttt cgccgggtat acactcaaaa attgttggaa cgatatcata
     9481 tctataaaca ttgatatgat atcgtagtct gagaataaat atatcattcc catgatgaca
     9541 aagaaaattc ccctagtgtg tttatctttg atatccgtta caatatctgc aatgacaaga
     9601 agaatgattc cattataatt tacataaaca aatttaataa tgatcagaca taacaaaatc
     9661 tctgctgcat ataagatcac agatacctgt tgcctttttc tttgttttct tcgaatttct
     9721 ataatgccta acaataagaa aaaagcaata aatatcaaca ctgttgatgt cactggttcg
     9781 tttgggagat atgtgatatt ctctaagaaa ttccttgctc caaaatcaca attgatctta
     9841 tttaatgtta aaataaaaac tgcagaaagg aaggataaaa tgatgaaatt tatgactccc
     9901 atcagaatct tgatataata taaattttga ttttttcgat ccatatcatt tcctctttac
     9961 tgccaacggc taatatcagt ctgctctaca ttcttttttg ttatcatttg aacagagatt
    10021 acctttttct tctctacttt ctccccttgc agaatctggt atgccgcatt gaccgctgtg
    10081 tctgccatct gtttgggaga ttgtgccgct gttcctgtca tcataccttc tttgatcagc
    10141 tttttccctt ctggcgaacc atcgattccg tatacaagaa ccccttctct tgcacccttg
    10201 ctgtcaagag ccgccaaagc tcccatcgcc gctgggtcat ttaatcccat gacaatatct
    10261 atgttctggt attcttcgat aatcttatct accttaggaa gtgcacgttc aatctgtcct
    10321 tgagtatctg cagatgcaag gatcttatag tttttattgt tcttgatcgt atctaaaaat
    10381 ccattgatac gatctaccgc agattttgct gcattatgtg ttaacaagat gatattcgcc
    10441 tgatcttttt gcttcatcat ttccttggca cataaaacgc ctgccttgta gttgtctgac
    10501 acgatcgtca ttgcgacgag atcacttctg taaacctgtg agtcgataac gatcacagga
    10561 atcttcgctt tctttgctgc tacaagtcct ggttctactg ctttccagtc tactggattt
    10621 agaaaaatcg catctacttt tcgtttgacc aactgtttaa tctgttcatt ctgcttcttc
    10681 tgatcgagtg ctggatctaa tgtgatcatc tggttcccat tcttctcaac ctgcatccga
    10741 atctggttat taactatctt atagaattca ttattcatcg tcatataagt tgcaccgaat
    10801 aatttttttc ttgtgttata ctttttcaaa tagtttgaaa atacacagac ataaattcca
    10861 ataaaaaaag caaaaactgt gattcctgct aaagcgacaa tcctattggt tagatttttt
    10921 tttgattgtt ggttcatatt tgtttactcc tcaattaaca taaaatcatc gctgctcatt
    10981 aagtgtccgc tatgtgcgaa tcgagcgaga gcgcgtttcc agatagcttt tatttctcta
    11041 caaccaagcc gttggactag catcactagg cattcgtaga tctccacgag gtgataccgc
    11101 cactctacct accttaggtc cgtctggcag acatgaacgt ttaaactgct ggctgaagaa
    11161 tctccagtaa aatttatcca gccatttctt gatcgtttca tcatcatata caccgtcaaa
    11221 tgttaatttt gccatgcggt atagtttagc ttttggaaat ccaaatcgta acatatagta
    11281 taagaagaaa tcgtgcagtt catatggtcc gactaagtct tctgtcttct gtgagatgac
    11341 tccatcaact ggtggcaata attctgggct gactggtgtg tctaggacat ccatcagtac
    11401 ttctgataat ttcttattct ctgttgtctc tgcatagtaa agtacaaggt aacgaactaa
    11461 tgtctttggt acggagcagt ttactgcata catagacata tgatccccgt tgtatgttgc
    11521 ccatccgagt gccagttctg acatatctcc tgttccgatg accataccat tgtactggtt
    11581 tgccatgtcc atcagaatct gggttctttc tcttgcctga gagttttcgt aagttacatc
    11641 atggttattt tcatcatgtc cgatatctgc aaaatgctgg cggactgctt tttcgattct
    11701 tacttctttg agtgtcgctc ctaattcttt aatcagtgat acggcattct ggtaagttct
    11761 gtctgttgtt ccaaaacatg gcattgtcac acagatcagg ttttctcttg gaatgtctaa
    11821 cagatcaaag gctcttgctg taactaagac tgctaatgtg gaatctaatc cacctgaaat
    11881 tcctacgact gcatgtttgc agtttgtgtg ttccagacgt ttcttaagtc ccatagactg
    11941 taaagataag atttcatcgc atcgtttatc tctttcatct tgattatctg gtacaaatgg
    12001 tgctttcggg aatgttcttg tgatcttgtt ttctttgact tctagtgaaa attcctgttc
    12061 aaaataatca tctgtttggc ctcctgggaa agaagtcatc tttcttcgtt ctgctgccaa
    12121 tttctgcaca tcgatatctg catagataat ctcatttgta aatcgttttg cttctgctaa
    12181 aactgaacca ttttcacaga tgagatggtg tccactgtat acaacatcct gtgtggattc
    12241 tccttctcct gcatcagcat agatataagc cgctaacaat cttgctgact gatttgatac
    12301 aaggcttctt cgataagatt cttttgttgt tgtctctaca cttgcagatg gattacagat
    12361 taccgttgct cctgccattg cgtgatatgt gcttggtgga agtggtaccc agagatcttc
    12421 acagatttca catgctacca caagttctgg aatctctttg catttgaata actgattcac
    12481 ggctacattt acataaccaa attcttctcc aaaccaaaga tcttcttcta atccttctcc
    12541 agatgtaaaa tgacgtaatt cataaaattc tgaatagttt ggtaaatgtg tttttggcac
    12601 gattcctaaa atactgccat cactgatcac aaccgcacag ttatataatt tttgttttat
    12661 aacgatcgga catcctacaa caactaacat actcttattt tcagttgctt tcacaattct
    12721 taaaagctga tttttacatt ctgttaaaag tggctgctgc aagaatagat caccacatgt
    12781 atatccactg atcgttaatt cagaaaatac cagaatactt gctccatttt tttctgtctc
    12841 atcgatgatc tttaaaattt cttctgtatt gtatgcagga tctgccactt tgatctttgg
    12901 tgttgcggct gcaactttga taaatccatc tttcattttg tatacctcaa ttaaatatat
    12961 gcaggggcca ggtttgatca cctgacccct tataaatctc tttgtattat tcttcttcca
    13021 tgttacttaa ttttgctgcc tgatctgctg ctgttaagct gtcgatcaat tcatctaaat
    13081 caccgttcat gatagaatct aatttgtgaa gtgtcagctt gattctgtga tctgtaacac
    13141 gaccctgtgg ataattgtat gttctgatct tttcagaacg gtctcctgta ccgatctggc
    13201 tctttctctt tgctgcttct tcatcatgtg ctttctgctg ttctaattca tacagacggg
    13261 aacgtaatac ctttaacgct ttgtccttat tctttaactg tgatttctca tcctgacagg
    13321 atacaacgat tcctgttggg atatgtgtta aacgtaccgc agagtctgtt gtattgacac
    13381 actgtccacc atttcctgat gcacggaata catcaaactt acaatcgttc atatcgattt
    13441 ctacttccac atcttccact tctggcatga tcgctaccgt aattgtagat gtatggattc
    13501 ttcctccaga ctctgttgca gggattctct gtacacggtg tacaccgctt tcgtacttga
    13561 atcttgagta tgcaccctgt ccagatacca tgaatacaag ttctttaaat ccaccgattc
    13621 cactctcatt aaaacttaat gtatttactt tccaacgttt gctttctgca taatttcgat
    13681 acatacgtgc aacgtcagcg acgaataacc cagcttcatc tccaccggca ccggcacgca
    13741 tctcaacgat gatattcttg tcatccattg gatcctttgg aattaagaga atctttaact
    13801 cttcttctaa tctttccaca tttgccttag attcattcag ttcttccttg gcaagttctt
    13861 tcatctcttc gtcagactct tcttctaaaa gcatcaaact atcttcgata ttctgtttct
    13921 ctgctttgta ttctttgtat ttttccacga tcggtgtcag ctcattttgt tctttcatca
    13981 gatcacggaa acgattctgg ttacctgcta catctggatc attcaattcc tgcagtacag
    14041 tgtccagacg atctactaaa ccttctaact tatcaaacat cttattctcc ttctcttgca
    14101 aatccaaaga ccagccggtc taatcctgcc aggtcctttt ttatctcaac acaggcaaaa
    14161 tcctgtgctt ccatcaaatt cttcactgct tctccctgat catatccgat ctcaaatgcg
    14221 agcattcctt gtggatttaa atagtttttc gcttctttga tgatcttctt ataaaaataa
    14281 agtccatctt ccttcccatc gagtgctaac atcggttcat agtcttttac ctccggcatc
    14341 agcttcccac attcttccgt tgtaatatag ggcggattcg aaatgatcag atcatagctt
    14401 cctgtaacat tctcaaaaag gtcactttga ataaatttta ccataggtgc gagattttcg
    14461 ccattagctt ttgcgatttt taatgctttt tcactgatat ctgtcccaat acatacttct
    14521 ggatttaaaa tcaatgccag actgatcgca atacagccag atcctgtaca gatatccagt
    14581 atcgtatctg gtttctttga ctgttttaac tgatcaagca cttcttccaa tactgtttct
    14641 gtatctgctc ttgggatcag tacatctggt gttaccttaa atgtatatcc cataaattcc
    14701 tgtgttccta aaatatattg cagtggaaca tgttcttttc tcttctgaat cagttcctta
    14761 tatctttctt ccgcttcttt atcattgatc tcatcggtca tcccaaacag gtagtcttcc
    14821 cttgatatat gaaaacattc ggaaaataaa taccatgcat caccggatgc attctcaatc
    14881 tgtgcttctt ctaattcctt ttgtccatag aggacccatt ctcttctatt catcttcttt
    14941 ttgttcccct ttatgaagat tttctaaata ttctctccaa tcgaagacac cttccaccga
    15001 tgcgatcgca acttcgatca tagaatgatc tggttctttc gttgtcagtt tctgaagtgc
    15061 cagtcctggt tttgagaaaa tctgtacaag tggatggtca ctgcttcctg caagtctgat
    15121 aaattcatag gaaacgccag ccaccaatgg taaaaataat aatcttaaca cgattcgtaa
    15181 ccagatagta tccactctta taaagaagaa aaataccata ctgatgatca tgacgataaa
    15241 aataaaactt gttccacatc ttttgtgtaa tcttgaaaag ttatccacat tttcaggagt
    15301 taaaggaaca cctgcttcta aacagttgat cgttttatgt tctgcaccat gatacatgaa
    15361 gactctcttg atgtcttcca ttcttgagat caacacaatg tatccaagaa atatcgctat
    15421 tcggataata ccttccataa ataaaatcac atattcattc ctgatcagct ttccgagtgc
    15481 ttcagatgcg atgtatggaa ggatcataaa taatccgatg gaaattgcca gtgaaaatac
    15541 cagtacgatt cccatgatga tcttctctgc tttctctcca agaatctttt ctaagagctg
    15601 ttctgtcctg ctctctgttt gttcttcttc gtcttcgtaa aaagatgcag aataattcag
    15661 tgtcttcatc ccgatcacca tagattccag gaaatttgcc attcctcgaa aaactggtaa
    15721 ttgaaataat ggatgtttct ctgcgaagct tacgcattct tctactttcg tctctatctt
    15781 gccattgggc tttcgcacgg atactgcata acgatccata ttcttcatca ttacgccttc
    15841 gatcactgcc tgcccgccta tacgaaccgc cattgttgtc tcctatattt ctatgagatt
    15901 tttctcgtta cataaaaaaa aggttgagat ttcgataacc tcaacctgat ttcttacttt
    15961 tctacttaac accatattta cgattgaact tatcaatacg tccacgagcc tgagtagctt
    16021 tctgctgtcc tgtgtagaat gaatgacatt ttgagcagat ttctacgtgg atatcttcct
    16081 ttgtagatcc tgttacgaat tcgttaccac agttgcatac tacttttgcc tgataataat
    16141 ttggatggat tccttcacgc atgttctttc acctcttcta aaaaatttgt tggattttgc
    16201 caacatttac atactttgac tttcgtcatt tcttgtttta taaacagctt ttttattcta
    16261 tcacaattta tttgaaattg caattacttt ttttattttc ttaataatga tcgtttggtt
    16321 agtgctacaa attctttatt gttttgggtt cgtttaaata aatctaacat ctgttccata
    16381 aattcttccg aacggtttcc ggataattct ttgtgaagtg ctgtgacaac ttctctttca
    16441 tctttggtaa gcagcagctc ttctcttctt gttcctgatt tggcaatatc aaccgctggg
    16501 aagattcttt tttctgccnn nnnnnnnnag actaattcca tattacctgt tcccttgaat
    16561 tcttcaaaga tcacgtcgtc catcttgctt cctgtttcta cgagtgccgt ggcaaggatg
    16621 gtaagacttc cgccttctct catatttctg gctgcaccga aaaatttctt cggcatatag
    16681 agagcggctg gatcaagtcc accagttaat gttcttccgc ttggcggtac tgtaaggttg
    16741 taagctcttg caagtcttgt gatactatct aataaaatta cgacatcttc tttatgttct
    16801 acgagacgtt ttgcacgttc taataccatc tctgacactc tcttgtggtg ttctggcaat
    16861 tcatcaaatg tggaataaat aacttcaacg ttctttcctt cgatagattc tttaaaatct
    16921 gtgacctctt ctggtcgttc atcgatcaat aatacgatta atttgatatc tttataattc
    16981 tgactgatac tttgtgccat ttgttttaac agtgttgtct ttcctgtctt tggaggagat
    17041 acgatcattc ctcgctgccc ttttccaacg ggtgatagca ggtcaaccat tcgcattggt
    17101 atggatgatc tctctgtttc cattcctaat ctttcgttag ggaagattgg tgtcagatct
    17161 tcaaaatgct gtcttctcgc tgccatttct ggcttatatc cattgacaga ttctacgaat
    17221 aataaggcac tgaatttctc actctgggtt ttgatacgga tcgctcctgt taagatgtct
    17281 cctgttttta aattgaattt gcggatctga gccggagata catagatatc attctcccct
    17341 ggaagatagt tttcacatcg gatgaaacca tacccgtctg gcataacttc cagaatccca
    17401 tgtgcttttt ctccgctgtc cagactcttt atattctttg gaaattcttt ctgatgatgt
    17461 tttctttctt gatgctctga ataagattgt tttggttcac gttgtatctg cggttcttcc
    17521 ttttgttttg attgttcctg atcatagttt tctaatactt tgatcagttc ttgtttcttt
    17581 aaggtcgaaa tgccttttat ttttctttct ttggcaattt cacgtaattc aattaccgac
    17641 tttttttcat atgtcattct atcttgctcc ttttacctgg gaaaatggat gaattatcct
    17701 ttaacgatcg atccagattt gtgttttctt tattctagca tagtttttca gatatttcca
    17761 atcatttgtc ttcttagttt ttttaagatc tttttttgta ttcttgaaac ctgtacctgt
    17821 gaaatatgaa agatcttcgc aatttcttgt tgagtttttc cttcaaaata tcggtaatag
    17881 atcatttgct gttctttctg ttccaaatgt ttcatggctt cctttaacag aacatgatct
    17941 accactttct cagattcatt ttctcctttt gataacttat ccagcagaga aatctcactg
    18001 ccatccttct gatatgcact ctgatgaatg gattctactt cattgcttgc ctctaaggat
    18061 gttacgatct cttctcttgt gatcttcgtg atctcactga tttcttcgat cgttgcttcc
    18121 ctttgatact gctgacaaaa cttttctctt gctttcatga tcttgtatac attttctttt
    18181 aaagatctgc tgactttgat cataccatca tctcttagga atctacgaat ctctcctgtg
    18241 atcatcggta ctgcataagt cgaaaatttc acgtcataag acaggtcaaa cttgtccacg
    18301 gctttcataa gtccaatact tccgatctga aacagatcct cagactcata tcctctgttt
    18361 gtaaatcgtc tcacaacact ccagatcaat ccaacattat taagaatgat ctgctctcgt
    18421 gctgcttcgt ttccttcttt ggtaagccgt aacagttccc tcgtcttatc cattcagctt
    18481 gtcttgatct cctattttct ttgtcatgat cactgtcgtt ccttctcctt gttttgaaat
    18541 aacctctact tgatccataa atgcttccat aaaagaaaat cccattccag tcctttcttc
    18601 ttctggtttt gttgtaaaca tcggttccat cgctttcttg acatctggaa ttccgatacc
    18661 ctgatcttta atctctattc gcaggattcc attctgatcg gaaagatcca tgctgatctt
    18721 tccatctggg ttgtgatacg catgtacaat acagtttgtc actgcttctg acactgcggt
    18781 cttgatatcg tccatttctt ccatatccgg atcaaatttc atgatataag cagccgtcac
    18841 aattcttgcg attccttcat tttctgagtc actgttaaat tctaaatgca acatttctta
    18901 tcctcccatt tctctatatt tcttctatca gttgatgcaa tcctgccata tgaatgatct
    18961 ttcttactga tgcactcata ttgctgactt tcaccgttcc tcccatcgcc tgtacctgac
    19021 gatatcttcc taagatcact ccaatccctg aactatcgat aaatcctgaa tttttaaaat
    19081 caaactcaat atctttgatc ggatatgatt ttaaaatgat atcacattta tgttttactt
    19141 tcttcgctaa gtgatgatct agatcaccat ccagataaat atataaaacc ctgttttcta
    19201 tcttgtattc tgttgccata ttctgatcgc tccttcctgc tctttgctgt gatacgttcc
    19261 tttccaagat catcttgaat ttcctgatag aaaataaaaa agaaagaagt tcaaactttc
    19321 gcttgttctt cttcctttat cttttgtttg taaacttgta ttctgtgtaa tattaattat
    19381 aaacttttgg cactcttttt acccattgca tcaattcgac tcttcgcatt gattgcctga
    19441 gatgttcctg ggaacttttc gatgatcgtc tgatatgtct tcttcgcatt cttctgatcg
    19501 gaagcacctt cataagcttt ggcaaggtaa tcatatgcat aacggtttgt atcgctgact
    19561 ttgatcgctt tcgttaaata agagatcgct gtcttgtagt cttttgccca gtatgcattc
    19621 tttcctttgc tgtataatcc agttcctgca cttggatata cctttgctgt cagatctttg
    19681 tagattgctt tcatattctt tgttgttagt ttcttactgt caatatctaa taacgcatct
    19741 gcagatcctg tataatcacg atcgctgtat ttctgtaagg cacttaacag cagatcatag
    19801 acaccgtctt tcttgcttgt tccaatgtac tcgtctaatt catcttctgc tttcttctgt
    19861 gctgcctttg cttctttaac tttcttctcg cttgctttca actgtgtgtc tttggcaagg
    19921 atctgttctt tataatcttc tacctgtgta gagttcttat gactgaagct tgcacgtact
    19981 gttggataga tcaaaactgc tgccacgatc acaccgatca caattcctgt taccatgtaa
    20041 aacatcgggc tctttccagc ttcgttttcc tgaattgaag aaatattaaa tggattcttc
    20101 tttttctttg gttcttctgg ctggctcaca acttttttct tctctttttt cttcttgtct
    20161 ttcttctctg gctcagttgt ctgtggctct tcttcgacta cttttgtctc aaattctttg
    20221 ccatattcca ctgctgattc gttgtttgga tcgatcttta agatcgtatc aaggaatgtt
    20281 cttgcattat cataattctc tcccttgatg gataataatg ccattaaaag atatgcatct
    20341 acaaaattag gaatagctgc cacaacattc tttaactgta acatcgcaag gtcatcattc
    20401 ccttgcttgg cgtagtttaa tgcctgattg tatttcttga tcatctggtt ggctttatat
    20461 aattctgtct tattcttctt gatctcttct aattctttcc catatatatt ctcttccgct
    20521 tcttccaaat ctgcacatgg ttgaaacttg gctaattctt cgttaaagtc taacattctc
    20581 ttcctcctga tttataaact ctttggccat gcctgccaga taaaaagatc cgacaacaaa
    20641 gcataccgtt gtttcctgat tcctgtctat catcttacga aaagcatcct gaaattctgc
    20701 ttcatcgatt cttctcttat gaatccccac tgtataaaag gcttcaatct ctggaatcgt
    20761 cttcagaatg ttcttcatgt attcttcttc attctgatga gatgctgcaa ataagatccg
    20821 cttcttcatt ccatggtagt ttgcctccac agtctgtaca aatccttgca ttccttggat
    20881 attgtgcgat acatccacaa tgatccctgg tgcgatctct tccatccttc cgctgatcct
    20941 gatctggctc agtgcttcat agatcttgtc ttcatcaagt cgtccacaga taagttctgc
    21001 tgtctgtaat gctgctgcaa gattggactt ctgaaattct cctgcaatgt tggtccgcac
    21061 atctctttcc ttatcatacg cattggcatt taaaaaatca atatattttc ctgctttttc
    21121 tttaaaatta agattatctt cagaaatgtc ataaattcgt gattttttct gccttgcttc
    21181 ttcagaaagg atttctttta ccttgggttc ctgttctatt gtcacaactg gaattcctgg
    21241 cttaatgatc cctgctttgt gtgttgcgat ctcttccaat gtttgtccca gaaattccat
    21301 atgatcttcg ctgattgaag tgatcacact gaccttcgga cagataacat tggtagcatc
    21361 atatcttcca cccattcctg tctcgatcat acagatatct ggttgttctt cctgaaagat
    21421 caccatcgcc atcagaaata ctgcctcaaa aaatgatggg ctgttctttc cttctgttat
    21481 tccagtttca atggcatgtc ttaccttcac cgcagctgat gcaaatcgtt catcactgat
    21541 ctctttcgca cacagcttga ttcgttcatt gatcttcaca acatgtggcg aggtaaagga
    21601 agctgtcttc tttccatgct gggtaaggag tgcttctaaa aatgcacaga cggatccttt
    21661 tccattcgtc cctgctacat ggatcatatg cagctctttt tgaggatttc ccaatcgttc
    21721 taaaaagtat tttatatctt cgagagaaga ttttgtggcg aatcctgcga ttttgtataa
    21781 atattttaca gactcttcat aagtcatggt catttcttct gttagcataa ttcaccactc
    21841 agtactgctt tcaatacctg aagtactcca tcttctttgt aagatggtgc tacaaaattc
    21901 gctgcttctt ttacttcatc tctggcattc tccactgcaa aactgtgact tgcacgtttt
    21961 aacatctcga tatcatttaa attatctcca aatgccagtg tttcatctgg tgtgattccg
    22021 tattcttcct gaaaatgtct taaggcactt cctttgttcg cacctttgtt ggcgcaatca
    22081 acccagcatt ttccggatac tacaacattc atcttcttgc cccatcgttt gatgaactca
    22141 tccccaacta catcttcggc gcaattgtga tgaaaaatac tcatcttaca tacaccttct
    22201 ggattcccta aaacatcatc tacaagatca cctctgtagc catagtctcc gaccagatgc
    22261 tggtaaatcc cgatattctc catatatgtc atgttatctt tattgatcgc aatctcacat
    22321 cctgggaact gtcttgcttc ttcaatataa gttcttacga tttctgggtc gatcacatct
    22381 acatattgat aatctttgga atgaatacaa gttccatttt ctgaaataaa actgatgtca
    22441 tcaagtactg gcgtgaatac tttctcgata cttgctctct gacggccgct tgccgctaac
    22501 acgatcgttc ctcttcgttt cagttctttg atcacgtcat aatattccgg attcagatcc
    22561 agtgtcccat ctgccactaa tgttccatca atgtctgttg cgattagttt gatcatttgt
    22621 tttcctttct tttctccata tacatgctgt atcattattt caaaaaggct gccatacttt
    22681 gtacagcaac ctttgtttta tttgtctttt tccagatcgt atggtgtcac ctgatataca
    22741 taataattca gccagttata atacatagca tttgcatgac atctccatga cttcactggt
    22801 ttcttcgttg gatcatcatc tttgtaataa ttctttggta tatctggatt cagtcctcgt
    22861 ttcacatccc ttacatattc ataatgcagt gacatcacat catattctgg atgtcctgtg
    22921 acaaatacct gacttcctgt ggaattcaaa ataatgtatg gtcctgtctc atctgattct
    22981 gctacgatcg ttaattcttt attcttgcgg atggcttctc cgtcaactcc tgtatttcta
    23041 gagtgtggca cgtagaactt atcatcaaat cctcgaataa taggaagttt attatgtaat
    23101 ggatggtgtt cataaacacc tgaaatcttc ttattcaaga tatgtttctt gatcccatag
    23161 tgataataaa gtcctgcttg tgctgcccag cagatataca aagttgatgt gacatgtgtc
    23221 ttggaccatt ccatgatctc aacaacttca tcccagtaat caacctcttc aaattctttt
    23281 aactctactg gtgctcctgt aatgatcagt ccgtcaaaac gtttgtcttt gatctctgag
    23341 aaataattat aaaatttctc catatgtgac tctgatacat gagtcggaac ataagattct
    23401 gtctgtaaaa atgtgatatc taactgtaat ggtgtatttg ataaacttct cagcaaatga
    23461 agttctgtgt ctaatttgtt tggcatcaga tttaaaattg caatctttaa aggcctgata
    23521 tcctgtgtac atgccctttc tttatccatc acaaatacat tttcttcttc taaggctgcc
    23581 ttcgccggca gatcgttgtc tattcgaatt ggcattttat caaatctcct tctctttttt
    23641 tcgattgctt tttaagctga cagcattttt cctgtcagat atttattata aaactcttgc
    23701 attttttatg caatctatat ttatcatcgt ccgatcagat taaggaaatc ttcttcgctc
    23761 agtattggaa tgtttaattc catcgctttc ttattctttg atgactgcga cattttgtca
    23821 ttgttgatca gatagtctgt tttctttgac actgatcctg tgaccttgcc gcccatctgc
    23881 tcaatctctt cttttagttg ttttctgtta tcaaaatgat caagtgatcc tgtcacaaca
    23941 aatgtttttc cttctaagat ctgttctgtc ttactttctt ctttcttctc aaactgcaca
    24001 tattgtaaca gttgttctaa gatcttctga ttatgtggtg tatgaaaata tgataccata
    24061 gcttctgcga tcatcggtcc aatctgatcg atcgcaataa aatcctctgc atcggcttct
    24121 ctgatcgcat taaaatcttc tttaaaatgt ttgcaaatca attttgcatt ggaaagtcct
    24181 acattcggaa ttcccagact ataaataaag tttgccaatg caggctgttt ggaatcattg
    24241 atcgcattga tcagattttg gtaggatttt tccccaaatc cttctaattc cagaatctct
    24301 tctttgtgat cttttaaagt gaagatgtca taaatttcat ttaatagtcc aacatcgatc
    24361 atcttctgta atgttgcttc ggatagtcca tcaacattca ttgcatctct tgatacaaaa
    24421 tgcgagaatg atttgatctt ctttgcactg caaaattcgt tggtgcagac tagggttctg
    24481 attccatttt catcttcgat ctttgtctca ccaccgcatg caggacaagt atgcggaatc
    24541 tctatattgc cgctctttgt tttattctct gcaagctgtg gaatgatcat attagctttg
    24601 tatacaacga tctcatcgcc gattcctaac tgcagttctt ccatcacact aatgttgtgg
    24661 agactggctc tgctgacagt cgttccttct aattctactg gttcaaaaat tgccactgga
    24721 ttgatcagtc cggttcttga tgggctccat tcaatctcag ttaatgttgt ctccgctgtc
    24781 tcatctgccc atttaaatgc gatggaatct cttgggaatt tggctgttct tccaagactt
    24841 tttccatatg caagatcatc atagactaat accagtccat ctgatggcag atcaaattgt
    24901 tcgatcttct ttgagaactt ctcaatctca cctcggatcg tatcttctgt gactctgata
    24961 tgttctacca catcaaatcc aaggcttgct gcccagtcta atttcttctc gaaggaatta
    25021 tcaaaatcca taccttcaac atcactgaca ctgaacgcat agaaatgaac gtggcgtttg
    25081 gctgtgatct cattgtttaa ctgtctgacg gaaccactgc ataagtttct tgggttctta
    25141 tactgactgt caacatcttc gatttcttcg ttcatcttat agaaatctga atatggaatg
    25201 atcgcttctc cacgaatgac caatgttccc tgatatgcaa tacgtcttgg aacattttca
    25261 aaaacccttg cgttgtttgt tacaacttct ccgacttccc catttccacg ggtcacggct
    25321 ttgatgagtt ctccatgttc ataggtcaga acgatcgtta aaccatcgag tttccatgac
    25381 aggattcctt cctggccgtt cagccatgct gccagctgat ctggctcctt tgtcttgtct
    25441 aatgataaca tcggagatgg atgtgcttcc ttttccaatg ctgtcatcaa ttcataccct
    25501 acatggatcg ttgggctgtt tgataaaaca gtgttcgtct ttttctctag ttctaaaagt
    25561 tcatcataca tcttatcgta ttcgaggttg ctcataactt ctttattctg ctggtaataa
    25621 gctttccctg cttcatttag tgtatgaatg agttctttca ttctgtctaa atcattcact
    25681 ttttagctcc ttctttaact ctcgtaattc atccggagtt aattttcgat atcttccttc
    25741 tttcagatcg ccgagtttaa tgttcataac acggactctc ttaagttttg tcacacggta
    25801 tccaaaatgt tcacacattc ttcggatctg ccggtttaat ccctgtgtca agatgatcct
    25861 gaactcgttt ggtccttctt ttataacttt gcatcttctt gttactgcat taatctcatc
    25921 taatggtact ggattcctca tatcatgaat gaattcattc gtaattcgtt tattcactgt
    25981 tacttcgtat tctttttcat gataatttct tgctttcatg atctgattgg ctgcttcacc
    26041 atcattggta agcaggatta atccttgaga atccttatct aatcgtccta caggatagat
    26101 ccttgttgga tagttaatat agtcgataat attatctttc tcttctttgc ttgctgtgca
    26161 tacaattcct cttggcttat gaaaagccag aaagatcaga tcttcctgaa tctttaccgg
    26221 ctttccatca aatgtcacgc tctgccccgg aagtactttg gttccgctta ctgcaacttc
    26281 tccatcgact tcgactctgc cttcctcgac ataagtatct gcctgtcttc tggagcagat
    26341 tcctgcttga cttaaatatt tatttatacg cactgcatct tctgttttac gttttgtctc
    26401 ttgcattgtt acctttccca cttgtcacaa agggcgatga tctgatcagc aaatttgccc
    26461 aggtctttgt tcgttccacc ctcattgtgc tcaattacag atttcaaacg ctcgctggct
    26521 gtaagaagtc tgatataagc actgctctgt tttggtttct tctggatgcg ttctcttgat
    26581 ccccattcag tccattcgcc tgcaataagg tcaaacacat ctccgctgta tggtgctctg
    26641 gctttgaatt tgtattcatc ttttaagcat gatgtaaatg cttcacagac cgcatcttct
    26701 ccatgaacta caaatacttc ttctggtttc ttctcgaaac tattgaccca ttttaataac
    26761 ccgttcttgt ctgcatgtcc gcttaaacct tctaattgta cgatatctgc tgccactttg
    26821 attgcctcac caaacagttt tacttctttg gctccttcga tcagtgatct tcccagagtt
    26881 ccatatgcct gatatcctac aaatatgatc gtattctctg atctccacag gttatgtttt
    26941 aaatggtgtc tgattcgtcc agcttcacac attccggatg ctgaaatgat caccatcggt
    27001 tctgtttcaa agttgatctc ctttgattct tctgtcgtaa tggataaatg aagattggaa
    27061 aatgtaatcg gattaattcc ttgtttcact aattccatag cttcctcatc atatgtttca
    27121 tcgatattca tattaaagat cttcgttgct tccactgcca atgggctgtc tacaacaact
    27181 ttgaaatctc catgattctt gatcctatct tcagccttga tctgacggat ataatataac
    27241 agctcctgtg ttcttccaac tgcaaatgat gggatcacga cattcccgcc tcggtcaaag
    27301 gttctctgga tcacattggt cagttctgtc acataatcct gatgttttcc atgataacgg
    27361 tcaccatacg tagattccat aataacataa tctgcttctc tcgtatagct cggatctttg
    27421 atcaatggct ggttaaagtt tccgatatct ccagagaata ccagtttctt ttccacatct
    27481 gcttccctta accatacttc aatgctggaa gatcctaaca gatgtccgat atctgtaaat
    27541 ttgatcgtaa tgccgtcagc aacttcaatc tgtctgccat atgggcatgg cacgaaatac
    27601 tgcatgacat tgatcgcatc atccatcgta tagatcggta caacttctgg ttttccagat
    27661 cgccttgctt tcctgttctt ccactccgct tcaaattcct gaatgtgtgc actgtcttgc
    27721 aacatgatct cacatagatc ggttgttgct cttgtcgcat agatctttcc tctgaaccct
    27781 tttgcataca acagcgggat cagtcctgaa tgatcgatat gtgcatgtgt cagcaggata
    27841 aaatcaagat tccctgcatt cactggaaga tcaacattct cgtatacatt taacccttgt
    27901 tccattccat agtcaacaat aaatcttttg ccgcaagctt ccacataatg gcaacttccg
    27961 gtcacttcat gatccgctcc gataaacatt aacttcatgg cttatacctc ctctgaaata
    28021 tagattgggc cagccggcac gatatctttt agatactcat gctcttttaa tatctgtcct
    28081 gcatcgtagt caaaaaacac ctgtgtcagc tcgtcgattg aaatttcatc ttcccatccc
    28141 tgtgcatccg ttgtgtgtat gtctaatgtc tcatcttgtg gtatgtctgc acgttgaatg
    28201 gatatcttat gattcagaca ttgaaactgg aatactccat tctgatcctt gatatatgga
    28261 tctttcacca atatcctgaa tgtaaaatcg ttctttcctt gcaagatctc tccccaagca
    28321 gtaaggttcg tgatccttgc catgatcttt ggtacttttt tgctttgtat aaaatccttt
    28381 cctattgaat ttcccttgaa taaatttcct tctgtgatat aaatcttctt acctttgtgt
    28441 cgttgtttga tctggttaat catatgttct ggctctaaag aatacgccca tcggatatat
    28501 acttcatctt cttcaaaact ttctgcaaat acgccttgca acagatcgtc ttcataataa
    28561 gctaaaaggc ttccatctcc actctggctt tctttttcca gtcgtctcag gtatggttca
    28621 tccggaactg tatatatctt gtattgttcc ataaaccact ctaaaaaatc ctggatcgtc
    28681 tgataatctt ctgccattat atgaacgatc ttatgatttc tatccgttgt atcatctgta
    28741 tatgaaatgt tatgatcatc gatcattgtt tcttcccagt ccatcacaaa tcgaaactgg
    28801 aatggttcat aatatgcttt attcgcaggc atcagatacg taaatggctg ttgctgctca
    28861 tgcatatcat tgaaacactt cttaagcatc tctgccatga tgcctcttct tctatattcc
    28921 ttccacaccg caactgctac aatataattt aatgtatatg tcttctttcc tgctcttatg
    28981 tgatatggat ttaaatggag cattcctaag atcttgttgt cttcttctgc cagtatcact
    29041 tgattcgttg catatatctc ttcaaaataa tactgtgcaa acggctctgg atcttcaaaa
    29101 atcttctgat acatgtcata tactggctgt ttgtctttgt ttttctgata aacgatctcc
    29161 atgctatttc ctttattctt attttgtttc attcagcaga cacactgcct gtgcactgat
    29221 cccgagtccc tctcctgtga atcctaaatg ttcttctgtc gttgctttga cattcacctg
    29281 atctggatct actttgaggg tcttactgat attttctttc attgttggaa tataatttaa
    29341 taactttggt ctctgtgcaa tgactgtcgc atccacattc ccaatctcgt atcctttttc
    29401 ctcgatcaat cttacaactt ctgctaacaa cttcatgctg tctgcattat gatatgcagg
    29461 gtctgtgtct ggaaaatgct ttccgatatc tcctaatgct gctgccccaa ggatcgcatc
    29521 tgtgatcgca tgcagcagaa catcagcatc tgagtgtcct aataatcctt tctcatatgg
    29581 tatatttact ccgcctaaga tcaagtctct tccttctact aactgatgga catcatatcc
    29641 cattcctaca cgcatattct tgctctcctt tttttgtttt tattataaca tgatttcact
    29701 cttttatcat gatttttagc agaaaaaaag acatattcat a
//
LOCUS       DAOMUE010000004        29026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_210_length_29026_cov_3.449449, whole genome shotgun sequence.
ACCESSION   DAOMUE010000004 DAOMUE010000000
VERSION     DAOMUE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 29026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29026
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210_length_29026_cov_3.449449"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1398)
                     /gene="dnaB"
                     /locus_tag="PLC71_00515"
     CDS             complement(58..1398)
                     /gene="dnaB"
                     /locus_tag="PLC71_00515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRM01162.1"
                     /translation="MDEAFIKKVQPNSPEAEQSVIGAMIMDRDAIADVIDIVNADDFY
                     QKQNGILFEAMTELYREGKPVDLVTLQNKLKKKDVPESMKSLEFIRDLLEVVPTSANA
                     KQYATIVREKATLRKLIKVTEDITTECYLGKDDVTEILDHTEKNVFGLLQQASGREEF
                     VPIDKVVLNTLDAIEEAASSAGRVTGIATGFTDLDYKLSGLHPSELIIVAARPAMGKT
                     AFVLNIAQKAAVRDHVPTAIFSLEMSKEQLVTRMMAMEAMVDSQSIRTGDLQETDWEK
                     IMESAGTIGRSPLIIDDTPGITIAELRSKCRRYKQIHGLDLIIIDYLQLMSGGKRSES
                     RQQEISEISRSLKALAREMNAPVIALSQLSRRVEERKPAKPMLSDLRESGSIEQDADV
                     VMFIYRDEYYNEDSEKKGIAEIIVDKQRNGSTGSVELVWLGQYTKFGNKERAPH"
     gene            complement(1411..1854)
                     /gene="rplI"
                     /locus_tag="PLC71_00520"
     CDS             complement(1411..1854)
                     /gene="rplI"
                     /locus_tag="PLC71_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRM01163.1"
                     /translation="MKVILLEDVKSVGKKDEVVEVNNGYARNVLFRKNLAIEATNKAL
                     NDLKLRKANDDKLAAEALADAKVLAKEIEEKQLVLKMKVGEGGRTFGSISTKEIAAEA
                     KKQLGYDLDKKKMHCEPIKNLGFHDVAIKVHPQVTATLRVKVEEK"
     gene            complement(1858..3933)
                     /locus_tag="PLC71_00525"
     CDS             complement(1858..3933)
                     /locus_tag="PLC71_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRM01164.1"
                     /translation="MDIMKKTKIEQYLQGPLWALLILAFVDIVFFFLDIRCGVILAVS
                     LVVFLVFQMIIYSRSKQEIIKECMDFMFEQSQVQNYLLQQLPVPYALLDGNGMIIWSN
                     DAFHKIVGENRGKSLQQIFPNLNRNLLPSDVMHSISHIEYKKKCKEGDKEKEQTLFYK
                     VEMNRIRVEDFSDENDNSCSRQMLKDNDLIAVYFFDQTLLKKYMEQNENQRMVIGLIY
                     IDNYEEILDDIDAVKRSMLIAVADRKINKFIQNVSGVVKRTERDKYFVVFEQKYLDKI
                     KENKFSILDEIKTINLGNSMHMTLSISFGINGNTYQENYEAACAGMDLALGRGGDQAV
                     IKDGEDISYYGGNCEVMERTTRVKARVKAHALKELLESKEKVVIMAHKIPDPDAIGAA
                     VGLYRLGLSLGRKAHIVMNEVTISVRAMVDELNKSGIYDEDMFIDNEQAIEITDENTL
                     LIVVDVNHANYTECEQLLSQTKTTVILDHHRKNKDMIKNPVLSYVEPYASSTCELVAE
                     ILQYVDSKPKLEPMEANAMYYGMLVDTDNFVNKTGVRTFEAAAYLKRNGADLTKVRKM
                     SRESMETYRIRAKAISEAEILYGRFAIATLVGIGVDSPTVIGAQVANELLDIDGIEAS
                     FVLTGVHERVYISARSIDEVNVQKIMEEFGGGGHLTVAGAQLVDVSLEEAKNIIHETL
                     RYLISKGEI"
     gene            complement(4192..4446)
                     /gene="rpsR"
                     /locus_tag="PLC71_00530"
     CDS             complement(4192..4446)
                     /gene="rpsR"
                     /locus_tag="PLC71_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRM01165.1"
                     /translation="MAYTKGDSPMRRRGGRRRRKVCAFCTEADAVIDYKDVATLKKYV
                     SERGKILPRRITGTCAKHQRAVTGAIKRARHLALMPYTAE"
     gene            complement(4463..4906)
                     /locus_tag="PLC71_00535"
     CDS             complement(4463..4906)
                     /locus_tag="PLC71_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRM01166.1"
                     /translation="MNKVVLMGRLTRDPDVRYSQGETPLAIARYTLAVDRRFKRNGEQ
                     DADFINCVAFGRTAEFAEKYLKQGTKMVVSGRIQTGSYTNRDGVKVYTTEVVIEEQDF
                     AESKAAAAQNSNMGVPQPSAPQPSAAASDGFMNIPDGLEEELPFS"
     gene            complement(4928..5215)
                     /gene="rpsF"
                     /locus_tag="PLC71_00540"
     CDS             complement(4928..5215)
                     /gene="rpsF"
                     /locus_tag="PLC71_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRM01167.1"
                     /translation="MMKYELALVLSAKIEDEERAAMVEKVQAMITTAGAAITNVDEWG
                     KKQLAYEIQKMKEGYYYFIQFEGETTVPAELEERLRIEETVLRFLCVKQDA"
     gene            complement(5308..5499)
                     /locus_tag="PLC71_00545"
     CDS             complement(5308..5499)
                     /locus_tag="PLC71_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HRM01168.1"
                     /translation="MEFQIGDIVKLKKAHPCGINEWEILRVGMDFRLKCVGCGRQVMV
                     PRKMVEKNFRGFIKKACQD"
     gene            complement(5625..5912)
                     /gene="spoVG"
                     /locus_tag="PLC71_00550"
     CDS             complement(5625..5912)
                     /gene="spoVG"
                     /locus_tag="PLC71_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HRM01169.1"
                     /translation="MQITDVRIRKVAKEGKMKAVVSITIDNEFVVHDIKVIEGEKGLF
                     IAMPSRKAADGEYRDIAHPINSDTRNMIQTLILEQYEAMNLGDIDATAPEV"
     gene            6214..7605
                     /gene="murC"
                     /locus_tag="PLC71_00555"
     CDS             6214..7605
                     /gene="murC"
                     /locus_tag="PLC71_00555"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRM01170.1"
                     /translation="MKMYQIDFNKPIHIHFMGIGGISMSGLAEILIKEGFSISGSDIH
                     TSDIIKHLEDKGAKVYLSQTADNITDDIDLVVYTAAINFDTNEEYKEAIRRNIPVMKR
                     ATLLGQMMHNYQNAIAVSGTHGKTTTTSMLSYILLAGNTDPTISVGGILDAINGNIRV
                     GHSETFVTEACEYTNSFLEFFPKISIILNIEEDHMDFFKDLDDIRHSFREFAHKLPDY
                     GYLIINGEIENIDYIVDGLKAEYATFGLENDSYDYCAKNIGYDAFGHAHFDYYYKGEF
                     IDHIQLNVNGEHNVKNALAAIAAAKRIGIDVDTMKKGLLGFTGAKRRFELKGTCNDFT
                     VVDDYAHHPTEIRATLESAKNYPHNELWCVFQPHTYSRTIAFLDDFAKALSLCDHVIV
                     TDIYAAREKDTGIVSSKDIVDRMADYDVDVHYIKEFDDIEKFILKNCKKNDLLITMGA
                     GNVDSIGNSLVNR"
     gene            7853..8944
                     /locus_tag="PLC71_00560"
     CDS             7853..8944
                     /locus_tag="PLC71_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HRM01171.1"
                     /translation="MNYIKVTTTYPTGGTLLPNAFVDNYMIRANGEYIKVYIYLLRMV
                     SDGANFSIPCLADVLELTERDVTRALKYWEKESLLRMELKDGEISFIELLPVSGNPHS
                     PAQTTKQEETAQATSSAASKTTVKDQTITGAEPIPAMKTLSPAELQRKSEDERFSYLL
                     YVCEMFLGKPLTCTDMNTLLYMYEDLRMSCELIEYLVEHCVSNGKKSFRYMQSVAINW
                     YEANIKTPEEAKAYSKNFRKEYYSIMRAFGLNQNPAPVQEEYMKRWLETDGFDLALII
                     EACNRTINAINKPSFPYADTILKHWKDNHIVTLEDAKNFHIRQKAPKTFANNNNQSNM
                     HNFTQRDYDFDALEQQLLQKQFADELMEN"
     gene            8948..9934
                     /locus_tag="PLC71_00565"
     CDS             8948..9934
                     /locus_tag="PLC71_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM01172.1"
                     /translation="MSLSNNDYQDIMRQYEERRLSNYRLHEQRKKDIYTTIPEIKELD
                     RQITANSISLGKQLIVRDDPALREEYRIKNQVLISQKQALLKEAGYSSDYLEPIYYCK
                     KCKDTGYIGQEQCSCFHQAMIDHLYSGSNMAKILARENFQTFREDYYSDQMTKQGLPS
                     PRRNIQKVVEHCKTFISRFPNHDNILFQGSTGVGKTFLSHCIAKEIMDRGFTCVYMTA
                     FQLFDTLAKHTFGKGKDMEQETSANLIFQCDLLIIDDLGTEMINKFVISQLFQCLNER
                     LIQERSTIISTNLSLQEFRNTYSERIFSRIIENYSWQKIYGDDIRFKKSNLT"
     gene            9989..11158
                     /locus_tag="PLC71_00570"
     CDS             9989..11158
                     /locus_tag="PLC71_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRM01173.1"
                     /translation="MRRSDRNFTKIPDGKLGIIALEGCKELGKTIDNYIIQWRSETYK
                     DFKDSVACDGYLRDTYLLDASCPRFGSGEAKGIIRESVRDMDLYIIVDVLNYSVTYSL
                     SGRVNHMSPDDHYADLKRIISASAGKAKSVNVIMPFLYESRQHKRSTRESLDCAVMLQ
                     ELISLGVDNILTFDAHDPRVQNAIPISGFDNIQPTYQFVKSLVEQCDDVHFDNDHLMV
                     ISPDEGAMQRAIYMANVVGVDVGMFYKRRDYSTVIDGRNPIVAHEFLGDSLDGKDVLI
                     IDDMISSGESMIDVAKELKRRNARRVFCLSTFGLFTNGLEVFDRAVEEGIIEKVITTN
                     LTYQTPELLSRDWYASSDVSKYVALLIDTLNHDASISDLLNPTERIKEYLEKKNM"
     gene            complement(11272..13488)
                     /locus_tag="PLC71_00575"
     CDS             complement(11272..13488)
                     /locus_tag="PLC71_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRM01174.1"
                     /translation="MAEETKETKKIIPESKKKHKKKHKKKKVLLKVSVVFLLLVVIGW
                     FTFGAKVMKLRSEAKKLVAQSSEETFKASQTSIVYDTNGKQLLKFKGEKDVYYLKYKE
                     LPVYVIAAVISVEDKNFYKHSGVDYKGISRAAVSYVVHKGRITQGGSTLTQQLAKTVF
                     LNRQKTWKRKVKEIFMAVELERKYSKEQILEFYLNNVYYANGYYGIQAASQGYFRKDA
                     KNLTMSEAAFLSAIPNNPTIYDPRTNKENTIKRRNKILKDMYEQKLIRKDQYEEAINE
                     EINVKSAQKSKTEKKNYVETYVIHCATKEIMKQKGFEFKYDFSSTSEKEKYQEEYDKM
                     YAECKRTLYSAGYHIYTSIDPDVQKQLQSSVDNALSGYTEKDKNGIYKTQASGTCIDN
                     DTGKVVAIVGGREQKNIGYTLNRAFQSPRQPGSAIKPLLVYAPALEHGWSAGSTVNDS
                     PMSTKDKNRVRNSHGSYSGQISLRRAVQKSSNVATMRIYEQLTPKTCLKYLEEMNFKY
                     LTDSDYKYYTTCIGGFTKGTTSEEMAAGYATLKNDGVYREPTCISKITTSDGDEVMSS
                     STKKRRVYSTNAANAMTDVLKSVVTGGTGVGAKVPNVDTAGKTGTTSLNKDGWFCGYT
                     PYYTTAVWVGRDDNRIMESLSGASYPKSIWSNFMNAIHSEYSSTDSMGGNYTDYQGET
                     TQQTGTQATTATESSTKGTEATTAAPTTAAPTTAAPTTAAPTTAAQQTEEQPAENQ"
     gene            complement(13624..16119)
                     /locus_tag="PLC71_00580"
     CDS             complement(13624..16119)
                     /locus_tag="PLC71_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01175.1"
                     /translation="MKQSKVIQISVFGILFLISLFIWNYMLVGRNNKGVTETSQATIP
                     LLTVSADGKNINQLHGYQGSVDASLIRDSITPIYENEVKVMLTYAKSDVSVVSYKIYD
                     TDNKTILGNGDTAFHKVKGKKTANIKIKERLENGKTYLMELSVKQNDKTIRYFTRVIY
                     GTSFHLKECLDFAQKFHDAALTEGNTEFISQYLETAEGTTSNNLANVDLKSTQEAVSY
                     ATLKPQVESVYPATVKEISENVTSLELRYILSAENTDGTKQYYQATEYFRVRYAKDRM
                     YLLNYERNMEAYMRYDTIDRSNNRILLGIGSSQKQLISKNGGKKAAFVVQDELWYYDY
                     QKSNMHKVFSFEGEDYRDSRNNYQKHAIQIMKMKNNGDIAFLVYGYMNRGKHEGENGI
                     SVYQYTASDQKIEELGFIPTQIQYDMMKNDIKKGAFLSGSGQFYFYLDGAVYQVDLDS
                     GKSKILAKNITDDMTVISENGMLALNQSKNKMKVVDLSTQKTEVITCKDDQVLRPIGF
                     IEKDFIYGVGNKKEVVRNKDGSYMYPLTNVYIRNNKKIVKEYGEDGTYITNTSVDGTT
                     IALTHSKKSGNGYKKINVSYIRYKDKQESKAVLEYGYTGTRLNQLYLAFPDDVYIQTR
                     PNYLSTNVEEQEDEIKIKFAVNDNKYRDAFVYTGGKLSGIYSNLKEAIKVAKQGGGVV
                     VNYHQMYLWEKGVAQNYGKGSNIPMIKAKNKDETDIACILMMARSEGIKISYDKIKKM
                     SGSTYDKLFKVFNKRAVNYSDCSMDDLVYAISKGRSFVAQRKNGTYVLVMSYNQTDLR
                     YYDPTNGKSVKANRKKLESEFKQAGSVYYGYAN"
     gene            complement(16297..16689)
                     /locus_tag="PLC71_00585"
     CDS             complement(16297..16689)
                     /locus_tag="PLC71_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01176.1"
                     /translation="MADRDGLGDLPLFDTEVRPVREFVAQEQRQEDDYQYITRMYSAL
                     ARQILDLIEDICDQMEYEGSRMFDEYMDRTMLLAMVDKIYDKIDHQEKQDCQKKEQLL
                     YQMILSLLGGEIYHRRCRYRRKRNMFKA"
     gene            complement(16753..17403)
                     /locus_tag="PLC71_00590"
     CDS             complement(16753..17403)
                     /locus_tag="PLC71_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HRM01177.1"
                     /translation="MRELELPDVQDVMLEYRKIKLLYRSALKEIGTKLEILNDEFKLV
                     HQYNPIEHIESRLKTDESITRKLIRKGKEITVENIQKYIDDIAGVRVICSFTPDIYRI
                     VDMISSQDDIEVLKTKDYMLNPKVNGYRSYHLIVRVPIFLSDRVVPTKVEIQIRTVAM
                     DFWASLEHKIYYKYEGNTPEYIKKELKECADLVTFLDSKMLAINEEVRSIREENKE"
     gene            complement(17520..17783)
                     /gene="ffs"
                     /locus_tag="PLC71_00595"
     ncRNA           complement(17520..17783)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLC71_00595"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(17872..19506)
                     /locus_tag="PLC71_00600"
     CDS             complement(17872..19506)
                     /locus_tag="PLC71_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HRM01178.1"
                     /translation="MERIKKLIVVILCVGMISSLNISFIYASQSNGYHPLNIKIESPD
                     VPKTVTPKRNARSLDSYYSSSELGQVTSVKDQGNTELCWAFGITSMGETSLIKQGIAS
                     TNIDFSEKHFGYFMYNRKNDILNNTKGDKTKVSGDWRYAGGNSLLAMLSLTGWYGLAK
                     ENIAPFNTGKWRLSDSVGQKDSAILKNGFFLGDTPQAAVVKSYIIGYGSVVMAYHAPE
                     ETWEETAYYGKNHEAYNCNSARQTANHIVAIVGWDDSYSRDNFNSGSRPSRDGAWIVK
                     NSWGNQEGSNGYTYISYEDKSLCEFVAGQFVKASEYKYNYFYDGSANPGILKLKKGQK
                     FANVFTAKKGSAKKKELIKAVNLVTWSANVKYSIQIYRNPKDGRPTSGTKMLKRPVTG
                     TIREAGTHTIDLSRKAEVLRGDKFAVVVKLRSSGKIGFDENDDYHWVSFVNKTKKGQS
                     YLYDHRKWNDLNPDHATMRLKAYTVMQPVNKIHLRYCKADSKNKNPKGIVLYYKGKHL
                     KKNKDYKIVKKEGHKYVIVKGRGRYRGTKKIYLKTK"
     gene            complement(19493..19978)
                     /gene="tadA"
                     /locus_tag="PLC71_00605"
     CDS             complement(19493..19978)
                     /gene="tadA"
                     /locus_tag="PLC71_00605"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRM01179.1"
                     /translation="MTQEEKYMKEAIKQAKKAAVIGDVPIGCVIVEDDKIIARAYNQR
                     NKKKTTLAHAELLAIQKASKKVNDWRLEDCTMYITLEPCQMCAGAIVQARIPKVVIGA
                     MNPKAGCAGSVLNILQIDKFNHQVEIERGILEEECSQMLSDFFKELRRRKKGDKEHGK
                     D"
     gene            20030..20929
                     /locus_tag="PLC71_00610"
     CDS             20030..20929
                     /locus_tag="PLC71_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6128 domain-containing protein"
                     /protein_id="HRM01180.1"
                     /translation="MKQKESTRLSDYQRIVSYLYEYHHGIKGSNVGFAKIEKRQNRLR
                     VYFHVKTNEQALEYKIYFYYFHQGAMNGIYLDTVKRNDAIIEFKNTYPLIDDFDKMDG
                     FLLYYSNDHFFGSQWKDHPIIIRSFVPVESAENKNENTEKTVPPEPSPETETLSETEA
                     SPTSSLSEYIEALQLEKDAKEFTHPNPPKDFDWKEYPTLPMPPSYSLDPSIKIGLEDL
                     DQIPDLDDNIKTNGFLLLNYGNFGHLMLAKDQDSGQRYLGIPGIFDNEKAFISRLFGF
                     KTFITVPLKPHKTGNFGYFILPV"
     gene            complement(20918..21775)
                     /gene="rsmA"
                     /locus_tag="PLC71_00615"
     CDS             complement(20918..21775)
                     /gene="rsmA"
                     /locus_tag="PLC71_00615"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRM01181.1"
                     /translation="MKLGNPKNTIEVIQKYDFDFQKKFGQNFLIDERVLEKIISAAEV
                     NKDDFVLEIGPGIGTMTQYLAENAREVMAVEIDKNLIPILSDTLSAYDNVSILNADIL
                     KVDIAKIVEEKNGGKPVKVVANLPYYITTPIIMGLFESHVPIDSITVMVQKEVADRMQ
                     SGPGTKDYGALSLAVQYYAEPYVVANVPPNCFMPRPKVGSAVIRLTKYKDAPIKVTNE
                     KLLFQLIRASFNQRRKTLQNGIKNFGGLNFSKEQVAQALEEMELPASVRGEALTLEQF
                     AQLSNILDR"
     gene            complement(21779..22552)
                     /locus_tag="PLC71_00620"
     CDS             complement(21779..22552)
                     /locus_tag="PLC71_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRM01182.1"
                     /translation="MIFDTHAHYDDKQFDQDREELLASMKDNGIGTIVDVGSNMETST
                     WIVEAVTRYPMMYGAVGVHPSDTAELKESDIDTLKKYAAMDRILAIGEIGLDYYWDEP
                     ERSIQKKWFEAQIELARDVKLPIIIHSRDAAKDTYDIMKALHAEEIGGVVHCFSYSKE
                     MARQFLDMGFYIGIGGVVTFKNAKTLKEVAAYTPLDRIVLETDCPYLSPEPNRGKRNS
                     SLNLNYVAEALSQIKGINKEELIAVTEENARQLYRMKEV"
     gene            complement(22549..23496)
                     /locus_tag="PLC71_00625"
     CDS             complement(22549..23496)
                     /locus_tag="PLC71_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRM01183.1"
                     /translation="MIEEEKNQFAVLIDADNISPKYAPIIFQELEAYGFPSCRRIYGN
                     WSKANGWNEELLLEYSIIPVQQFSYTSGKNATDMAMVIDAMDLLYGKKVDGFCIVTSD
                     SDFTRLAMRLREEHMYVIGMGESKTPVALTRACNKFIHLNLIYEADRGKEEDMESDEI
                     ENVTSLDEISYTIADIISENGNAPVDLGKIGNRLNAKYSDFDVRNYGYTKLSVLIDEE
                     MDNFMLVQEDTNCYVEFKELTSRETIEEEVIHFVKKNGGSLDNLSAVTLELKDKHSGF
                     NIKDYGYSRMSSFLRSIRCLTVNGNTVRLKKRREKGERR"
     gene            complement(23590..23660)
                     /locus_tag="PLC71_00630"
     tRNA            complement(23590..23660)
                     /locus_tag="PLC71_00630"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23626..23628),aa:Gly,seq:tcc)
     gene            23799..24371
                     /locus_tag="PLC71_00635"
     CDS             23799..24371
                     /locus_tag="PLC71_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01184.1"
                     /translation="MMNKIWCFMILAGLIVSSFNGKITTLSDTILSSSKEAVELCILM
                     FGIVGLWSGLMNIALSLGITTQLQKLLTPFLTFLFPNLKNQKAKEYISTNIVANILGL
                     GWAATPSGLKAMEELQKDNPDKNTATNEMCSFLILNISSLQLIPINIIAYRSQYGSVN
                     PSKILIPGLIATLGSTIVAVIYIKWKDRKR"
     gene            24368..24883
                     /locus_tag="PLC71_00640"
     CDS             24368..24883
                     /locus_tag="PLC71_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRM01185.1"
                     /translation="MITFLANLVIPLFIFTLVLYGTLKRKDIYTPFLEGVMDGFKIVL
                     EIAPTLIALFFAIQIFRSSGALDLIVRFLTPMGKLLKIPKEVLPVIFAKLFSSSAATG
                     FLLDIYKTSGPDSLAGFMSSVILSSTETCFYTLSVYYSVVGIEKIRYTLTGALLAVFV
                     GTFISVFISYF"
     gene            complement(25004..27133)
                     /locus_tag="PLC71_00645"
     CDS             complement(25004..27133)
                     /locus_tag="PLC71_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HRM01186.1"
                     /translation="MILGHTIKDYKRENLLHDVITGIIIMAVSIPISMGYSQIAGLPA
                     VYGLYGSVFPIILFALFSTSPQFIFGVDAAPAALVGSALLGMNIQSGSKEALAVVPVI
                     TFFVALWLLAFYLMHAGKLVNYISAPVMGGFITGICSTIILMQVPKLMGGTAGTGELL
                     ELLEHIEKTAEQINLPSLILGILSLVVLLAAKKIMPKFPMAVVMMAAGAVLTQVLPMK
                     EWGIKTLDAVAPGLPKWSFPNLMLIPMDEVVTISLSVAVVIMAETLLAENNFAQKNGY
                     RINDNQELLAFAVGNLVAALTGCCPINGSVSRTAMGEQYQGKTQLMSIVAGLSMILLL
                     LFGTGFIGYLPVPVLTAIVISALLGATEFELAVKLWKVSRTECFIFWGAFIGVLLLGT
                     INGVLIGIILSFAEMVIRSSKPASCFLGIQPGHKHFRDLAESRQIHEIEGVLIYRFSS
                     NLFFANAGVLQQDIEQELERRGNIKAVILDASGIGSMDITAADRIEILYKSLKEQGIR
                     FYITEHIADLNEQMRKLGIGYLIEEGCVRRTIYIALKDMGIKRPYPLEGGVDNEERSA
                     SRKRVDNRMQEFVWAFGSDTEDQIERQIAQQIKQLKQNGDVENLLHGSWSHMEAFDED
                     EWLEHLEEHLKEIVNISGKDERTLAARFEEHRREIHERIMKEHPELAQRFKERRHRLD
                     EHLKERRPEVYDLVVSLRELEKRKDME"
     gene            complement(27130..28263)
                     /locus_tag="PLC71_00650"
     CDS             complement(27130..28263)
                     /locus_tag="PLC71_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRM01187.1"
                     /translation="MSKYKIMTPGPVQVPENVRLARSLSTTNADLDPQFYDEYKETCE
                     LISELLHTKNETLILSGEGILGLEAACASMTEPGDRVLILDNGIYGAGFADFVSMYGG
                     TPVMYTKDYKNAFDVDELDAYLKEDHDFKYATVVHCDTPSGMLNDIHKICPLLKSYGI
                     MTVTDSVSGMFGNEVNVDKAQIDILCGGSQKAVSAPPGLTFVTISDEAKKAMENRKTP
                     IASFYCNLTIFKDYYKNLWFPYTMPISDIYGLKAAMDNIAADKDLIKRHARIANATRK
                     AIEAFGLKLHLESGFSDTVTVFDVPEGMTADDILERMREKHGIMLAGSFGELAGKVIR
                     IGHMGASANVGDMLEVMSGLEETLRYLGWKSENSLLGVFHTEL"
     gene            complement(28362..28883)
                     /locus_tag="PLC71_00655"
     CDS             complement(28362..28883)
                     /locus_tag="PLC71_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM01188.1"
                     /translation="MKENKVQFIAITAMAVALTYVFTAFVNVRLPIAANGGLIHLGNV
                     PLFIMAILFGKKTGAIAGGVGMGLFDLLSGWTAWAPFTFIIVGIMGYVVGVMTEKHDQ
                     YGWKVAAIFAAFVIKIVGYYIAEIILYGNFIAPISSVPGNVVQIAVAAVVVLIVIEPI
                     EIAVKKIGITQFA"
BASE COUNT         8036 a         6412 c         4545 g        10033 t
ORIGIN      
        1 ctttgtttta tttgtatatt ttatccaatt atacatgtaa gaaaaagcac ccaaaaatta
       61 atgtggtgct ctctctttat tgccaaattt ggtgtattgt ccgagccata caagctctac
      121 actacctgtt gaaccatttc gctgtttgtc gacgatgatc tctgcaattc ctttcttctc
      181 ggaatcttca ttataatatt catcacgata aataaacata acaacgtcgg catcctgctc
      241 gatcgatcct gactcacgaa gatctgatag cattggcttg gctggttttc tctcttcaac
      301 tcttcgactt agctgagata atgcgatcac tggtgcattc atctctcttg ctaatgcttt
      361 taacgatctt gagatctctg aaatctcctg ctgtctggat tcagaacgct taccacctga
      421 catcaactgc aggtagtcga taatgatcag gtccagtcca tgaatctgtt tgtatctacg
      481 acacttagat cgtaattctg caatcgtaat acctggtgta tcatcaatga tcaatggaga
      541 acgaccgatc gttcccgcac tttccatgat cttctcccag tcggtttcct gaagatcacc
      601 agtacggata gattgtgaat ctaccatcgc ttccatcgcc atcatacggg ttacaagctg
      661 ttctttcgac atctccagac taaagatcgc tgttggaacg tgatccctga ctgccgcttt
      721 ctgtgcgata ttcaacacaa atgctgtctt acccatagca ggacgtgccg ccacgatgat
      781 cagttctgat ggatgtagtc cagataattt gtaatcaaga tctgtaaatc ctgttgcaat
      841 acctgtgact cgcccagcag aacttgccgc ttcttcgatc gcatccagtg tatttaacac
      901 aactttgtca attggaacaa actcttctct tccacttgcc tgctgaagaa gtccgaatac
      961 attcttctct gtgtgatcca agatctctgt cacatcatct ttaccaagat aacattctgt
     1021 tgtgatatct tctgtaactt tgatcaattt acgaagtgtt gccttttccc tgacgatcgt
     1081 tgcgtattgt ttggcattgg ctgacgttgg aacaacttcc agaagatccc tgataaattc
     1141 aagactcttc atagattccg gcacgtcttt tttctttaat ttattctgaa gcgttaccag
     1201 atcgaccggc tttccttccc ggtataattc tgtcatcgct tcaaataaga tgccgttctg
     1261 cttctgatag aaatcatcgg cattcacaat atcgataaca tctgcgatcg catccctgtc
     1321 catgatcatc gcaccgatca cagactgttc tgcctccggg ctgttcggct gcactttctt
     1381 gataaatgct tcatccataa atatgccctc ttatttttct tctactttaa ctcttaatgt
     1441 tgctgttacc tgtggatgaa ctttgatcgc aacatcatgg aatccaagat tcttgattgg
     1501 ttcacaatgc atttttttct tatctaaatc atatcctaac tgtttctttg cttctgctgc
     1561 gatttctttt gtggaaatag atccaaatgt tcttccacct tctccaacct tcatctttaa
     1621 taccaattgt ttttcttcaa tctcttttgc taaaactttg gcatctgcta atgcttctgc
     1681 agccagttta tcatcatttg cctttcttaa ttttagatca tttaatgctt tatttgtagc
     1741 ttcaattgct aaattcttgc ggaataatac atttcttgca tatccgttgt taacctctac
     1801 tacttcatct ttttttccaa ctgattttac atcttctaat aaaataactt tcatcgctta
     1861 tatctctcct tttgatatca aatatcgtaa tgtctcatga ataatattct tggcttcttc
     1921 taatgataca tcaacaagct gtgcacctgc aactgtcaaa tgtccgccgc ctccgaattc
     1981 ttccatgatc ttctgaacat tcacttcatc gatcgatctt gcactgatat agactcgctc
     2041 atgaacacct gttaatacaa aagatgcttc aattccatca atatcaagta attcattcgc
     2101 tacctgtgca ccaataacag tagggctgtc cacaccgatt cctaccagtg ttgcgatcgc
     2161 gaacctgcca tataagatct ctgcttcact gatcgctttc gcacggatcc tgtaagtttc
     2221 catgctttct cttgacatct ttcttacctt tgtaagatca gctccatttc tctttagata
     2281 tgccgctgcc tcaaatgttc tgacacctgt tttattcaca aaattgtccg tatctacaag
     2341 cattccataa tacatggcat tcgcttccat tggctctaac tttggttttg aatctacata
     2401 ttgaagaatc tctgctacca attcacatgt agaagacgca tatggctcta catatgaaag
     2461 taccggattc ttgatcatat ctttattctt tcgatgatga tctaagatca ccgttgtctt
     2521 tgtctgactt aataactgtt cacattctgt ataatttgca tgatttacat ccactacgat
     2581 caataacgtg ttttcatctg tgatctcaat tgcttgttcg ttatcaatga acatatcttc
     2641 atcataaata ccacttttat ttaattcatc aaccatcgca cggacagaga tcgttacttc
     2701 gttcataacg atatgtgcct ttcttcccag agaaagtcca agtctgtaca gaccaacagc
     2761 tgcaccgatc gcatctggat ccggaatctt atgtgccatg atcacgacct tttcctttga
     2821 ttcaagaagt tcttttaacg catgcgcttt aactcgtgct ttgactcgtg tggtacgttc
     2881 cataacctca caattgccac catagtagga aatatcttcc ccatccttga tcactgcctg
     2941 atcaccacca cgtcctaatg ccagatccat acctgcacag gctgcttcat agttttcctg
     3001 atacgtattt ccattaattc caaaactgat gcttaatgtc atatgcatag aatttccaag
     3061 attgatcgtc ttgatctcat caaggatcga gaatttgttt tctttgatct tgtccaaata
     3121 tttctgttca aatacaacaa aatatttatc tctctctgtt ctcttcacaa ccccagagac
     3181 attctggata aatttgttga tcttacgatc tgccactgcg atcagcatag atcgtttcac
     3241 cgcatcaata tcatcaagga tttcttcgta attatcaata tagattaaac caatgaccat
     3301 tctctgattc tcattttgtt ccatatattt cttaagtaat gtctgatcaa aaaaatatac
     3361 tgcgatcaga tcgttgtcct taagcatctg tctagaacaa ctgttatcat tctcatcaga
     3421 gaaatcttct acacgaatac gattcatctc tactttatag aatagcgtct gttctttttc
     3481 cttatctccc tctttgcatt tctttttata ttcaatatga gaaatagagt gcatcacatc
     3541 cgacggaagc agattacggt tcagattcgg aaaaatctgt tgcaatgact ttcctcggtt
     3601 ctctcccaca attttatgaa atgcatcatt tgaccatatg atcattccat tcccatcaag
     3661 cagtgcatac ggaacaggaa gctgttgtaa caaataattc tgcacctgac tctgctcaaa
     3721 cataaaatcc atacattctt tgatgatctc ttgtttgctt cgggaataaa tgatcatctg
     3781 aaaaactaaa aatacgacta gtgagaccgc taagatcact ccacatcgaa tatccaagaa
     3841 aaagaacaca atgtctacaa aagccagaat aagcaatgcc cataatggtc cttgaagata
     3901 ctgttctatc tttgttttct tcataatgtc catttttttc tcctgatttg attcttacaa
     3961 ttatccttta ttataactca attgcatgaa tttgtacatt atatttaacg aaagcgagat
     4021 atttgttcaa tttacgcaat tttcttaatt attttgacat gtctgtcgca gtgcgatctt
     4081 gcccggaggg ctttttgtat cataggggat tccatcggaa tttctatgat acaaaaaaag
     4141 agctataccg caacatacgt cacaatataa ctcttcatta tctgcttaaa attattcagc
     4201 tgtatatggc attaaagcta aatgtctagc tctcttaata gctcctgtaa cagctctctg
     4261 atgtttagca catgttcctg taatacgtct tggaaggatt tttcctcttt cagatacgta
     4321 tttctttaat gttgctacat ctttataatc gattacagca tcggcttctg tacagaatgc
     4381 acatactttt cttcttctac gtccgcctct tcttctcatt ggagaatcgc cttttgtata
     4441 agccatgatt tctacctcct atttaactaa atggtaattc ttcttccaaa ccatccggaa
     4501 tattcataaa accatcgctt gctgcggcac taggctgtgg cgcgcttggc tgtggaactc
     4561 ccatattact gttctgggct gcagcagctt tgctctctgc aaaatcctgc tcttcgatca
     4621 caacttctgt tgtgtaaact ttaactccat cacggtttgt ataactacct gtctggattc
     4681 ttccagaaac aaccatctta gtaccctgtt ttagatactt ctctgcaaat tccgctgtgc
     4741 gtccgaatgc cacacagtta ataaaatccg catcctgttc accgtttctt ttgaatcttc
     4801 tatctactgc taacgtgtat ctggcgattg ccaaaggtgt ctcaccttgt gaatatctta
     4861 catcagggtc ccttgtcaat cttcccatta atactacttt attcattatt cggatccctc
     4921 ctgttaatta cgcatcctgt ttaacgcata agaaacgtaa tacagtttcc tcaatacgaa
     4981 gtctctcttc aagttctgct ggaactgtag tttcgccttc gaactggatg aaatagtagt
     5041 atccttcttt catcttctga atttcgtaag ctaactgttt cttaccccat tcatcgacgt
     5101 tagtgatcgc agcacctgct gtagtaatca ttgcctgaac tttttcaacc atagctgcac
     5161 gttcttcgtc ttcgatcttt gcacttaaaa ctaatgctaa ttcatatttc atcattactt
     5221 gcacctcctt ttggtctagt ggctctttct tcgtgaaaga gcaaggatat ttttatatac
     5281 cacgaataat tactctatca taaacttcta atcttggcaa gcttttttta taaatcctct
     5341 aaaatttttt tcaaccatct ttcttggcac catcacctgt ctgccacatc caacacattt
     5401 taaacgaaaa tccattccca ctctcagaat ctcccattca tttataccgc aaggatgtgc
     5461 tttttttaat ttgacaatat ctccgatctg aaattccatc catcaaacct ccttgttctg
     5521 atctatcttt tacactatag ttctgattat aacagaaaaa agaaccaggt ggtcaatacc
     5581 cgattctttt tctacacttt gaagaagaag ttattctctt aatcttagac ttctggcgct
     5641 gttgcgtcaa tatcccccag attcattgct tcatattgtt ctaaaattaa agtctggatc
     5701 atatttcttg tatcagaatt gatcggatgt gcaatgtctc tatattcacc atctgcagct
     5761 tttcggcttg gcattgcaat aaacagacct ttctcccctt cgattacttt aatatcatgt
     5821 accacaaatt cattatcgat tgtgattgac acaacagctt tcatcttacc ttctttggcc
     5881 acttttctaa tacgtacatc agttatttgc atgttgtcat cctcctagtt tctaataata
     5941 tagctaaaat tcgctctccg gtcaggctct ggtaacttct tctttctctt agaccgtata
     6001 tgctaatttt tattataaca ctattttcag caaatgtgaa caaaaacttg ttttttatca
     6061 gcttttttat tatgacattt tactactttt ttctccatat tcttatagta catataatat
     6121 tttccaattt aatctccgtc ttgcaagatt agttgcggtg acttataata aggaaaacaa
     6181 tgaaactata tcaacgattg aaagaaaggg cacatgaaaa tgtatcagat agattttaat
     6241 aaaccaattc atattcattt tatggggatc ggaggaatca gcatgagcgg tcttgctgag
     6301 atccttatca aagagggctt ttcgatttct ggatccgata tccatacttc cgatatcatc
     6361 aaacacttag aagataaagg cgcaaaggtt tatctcagcc agactgctga taacattaca
     6421 gatgatatcg atcttgttgt atacacagca gcgatcaact tcgataccaa cgaagaatat
     6481 aaagaagcca tccgccgtaa cattcctgtc atgaaacgtg ctacactgct tggtcagatg
     6541 atgcataatt atcagaatgc gatcgccgta tctggaactc acggtaagac aacaacaact
     6601 tctatgttat cttatatttt acttgctggt aacacagatc ctacgatcag cgttggtgga
     6661 attcttgatg cgatcaatgg gaatatccgt gtcggacatt ccgagacatt cgtaactgaa
     6721 gcatgtgaat acacaaacag tttcttagaa ttcttcccta agatcagtat tatcctcaat
     6781 attgaagaag atcacatgga cttctttaag gatcttgatg atatccgcca ttctttccgt
     6841 gaatttgccc acaaacttcc tgattatggt tatctgatca tcaacggtga aattgagaac
     6901 attgattata ttgtggacgg attaaaggca gaatatgcaa cttttggatt agaaaatgat
     6961 tcttatgact attgtgcaaa gaacatcggg tatgatgcat ttggacatgc tcatttcgat
     7021 tattactata aaggagaatt tattgaccat atccagttaa atgtcaacgg agaacataac
     7081 gtgaagaatg cactggctgc cattgctgct gcaaaacgta tcggcattga tgttgataca
     7141 atgaagaaag gacttcttgg attcactggc gccaaacgtc gttttgaact aaaaggcaca
     7201 tgcaacgact tcaccgttgt tgatgactat gcgcatcatc caacagagat ccgtgcaact
     7261 ctggaatctg ctaagaatta tccacataac gaattatggt gtgtattcca gcctcatact
     7321 tattccagaa caattgcatt ccttgatgac tttgcaaagg cattaagtct ctgtgaccac
     7381 gtgatcgtca ctgacatcta tgctgccaga gaaaaagata ctggtatcgt aagttccaaa
     7441 gatatcgtgg accgtatggc tgattatgat gtagatgtac actatattaa ggaatttgat
     7501 gacattgaaa aatttatttt aaaaaattgc aaaaaaaatg atctgttgat aactatgggg
     7561 gccggaaacg tcgattctat cggaaattcg ctcgtaaata gatagttatc cacattatcc
     7621 acagacttgt acacaattgt tgataaacaa atctgtggat ttcttttgtc cttttccaca
     7681 attgtaaaat ctatgtaaat ggcttatttc cattgtttta cgatttttgt ccacattatc
     7741 cacattatcc acagacttgt acacaatcag ggtgtggatt tccctggtat ttacaatttc
     7801 tccaatctgc gttatagtca gattaacgat aataaaagaa ggagagattg ccatgaatta
     7861 cattaaagta acaaccacat atccaaccgg tggaacgctg cttccaaacg catttgttga
     7921 taattatatg atccgtgcga acggtgaata catcaaagtg tatatttatc ttctgcgtat
     7981 ggtttctgac ggtgcaaact tcagcattcc ttgtcttgca gatgtactgg aacttacgga
     8041 gcgtgatgtt actcgtgcgt taaaatactg ggagaaagaa tccctcttac gcatggagct
     8101 taaagacggc gagatcagtt ttatagagct tctccctgtc agtggcaatc cacattctcc
     8161 tgcacagact acaaaacaag aagaaacagc ccaagctact tcttcagcag cttctaagac
     8221 tactgtgaag gatcagacga tcactggtgc tgaaccgatt cctgccatga agacgctttc
     8281 tcctgcagaa cttcagcgta agagcgaaga tgagcgtttt tcttatcttc tctacgtatg
     8341 tgagatgttc ttaggaaagc cattaacttg tacggatatg aacactttac tttacatgta
     8401 tgaagatctt agaatgtcat gtgaattgat cgaatatctt gtagagcatt gtgtatcaaa
     8461 tggtaagaag agtttccgtt atatgcagtc tgtagcgatc aactggtacg aagcaaatat
     8521 caaaactcct gaagaagcga aagcatacag caagaatttt cgtaaggaat attattctat
     8581 catgcgtgca tttggattaa atcagaatcc agcccctgtt caggaagaat atatgaaacg
     8641 atggcttgaa actgatggtt ttgatcttgc actgatcatt gaagcttgta atcgtacgat
     8701 caatgcgatc aacaaaccta gttttccata tgcagatacg atcttaaagc actggaaaga
     8761 taaccacatc gtaacattgg aagatgcgaa aaacttccat atccggcaga aagcaccaaa
     8821 gactttcgct aacaataaca accagagcaa tatgcataac tttacgcagc gtgattatga
     8881 ttttgatgcg ttagagcaac agcttttaca gaaacagttc gcggatgaac tcatggagaa
     8941 ctaacttatg tcattatcaa ataacgatta tcaggatatc atgcgccagt atgaggaaag
     9001 acggctttct aattaccgtc tccatgaaca gcgcaagaaa gatatataca caacaattcc
     9061 agagattaaa gaacttgacc ggcagatcac tgccaattct atttctctcg gaaagcagct
     9121 gatcgtccgg gatgatcctg ctttgagaga agaataccgt ataaagaatc aggtgctgat
     9181 cagccagaaa caggctctct taaaagaagc cggatattct tctgattatc tggaaccgat
     9241 ctactattgt aagaagtgta aggacaccgg atatattgga caggaacaat gttcctgctt
     9301 tcatcaggcg atgatcgatc acctgtattc cggttctaat atggctaaga ttttagcacg
     9361 ggagaatttt cagaccttcc gggaagatta ttattccgac cagatgacca agcaaggtct
     9421 tccaagccct agacgtaaca tccagaaagt cgtggaacac tgtaaaacct ttatttccag
     9481 atttccaaat catgacaaca ttttatttca gggttccact ggtgttggga agactttctt
     9541 aagtcattgt attgcaaagg aaatcatgga ccgcggtttt acctgcgtct atatgacagc
     9601 tttccagctg tttgatactt tggcaaagca tacctttggc aaaggaaagg atatggaaca
     9661 ggaaacatct gctaacctga tctttcaatg cgatctattg atcatcgatg accttggaac
     9721 tgaaatgatc aataaatttg tgatctcaca gttatttcaa tgtttgaatg aacgattgat
     9781 ccaggaaaga tcaacgatca tatccacaaa tttatctctg caagaattca gaaatactta
     9841 ttctgaacga attttttcaa ggatcattga gaattattca tggcagaaaa tctatggaga
     9901 tgatatccgt ttcaaaaaat cgaacttgac gtaacttatt tataatgtta taaaaaacta
     9961 tagaacaata tacgaaggag atagtatcat gagacgatct gaccgtaact ttactaagat
    10021 tccagacggt aaacttggaa tcattgccct tgaaggatgc aaagaacttg gaaaaacaat
    10081 cgacaactat atcatccagt ggagatctga aacttacaaa gatttcaaag actctgttgc
    10141 ctgtgatgga tatttaagag atacttatct tttagatgcc agttgcccac gttttggttc
    10201 tggagaagct aaaggtatca ttcgcgaatc tgttcgtgac atggatttat acattattgt
    10261 tgatgtatta aactacagcg taacttactc tttaagcgga cgtgtaaacc acatgtctcc
    10321 agacgatcac tatgctgact taaaacgtat catcagtgct tccgctggaa aggctaagag
    10381 tgttaatgtt atcatgcctt tcttatatga aagccgtcag cataagagaa gtacacgtga
    10441 atctttagac tgtgcagtta tgcttcagga actgatcagc cttggtgttg ataatatcct
    10501 gactttcgat gcacatgacc ctcgtgtaca gaatgcaatc cctatcagtg gatttgataa
    10561 catccagcct acttatcagt ttgtaaaatc attagttgaa cagtgtgatg atgttcactt
    10621 tgacaacgac cacttaatgg ttatcagccc agatgaaggt gctatgcagc gtgcgatcta
    10681 catggcaaac gttgttggtg ttgatgttgg tatgttctac aaacgccgtg actattctac
    10741 tgtcattgac ggacgtaatc ctatcgttgc acatgaattc cttggtgact ccttagatgg
    10801 taaggatgtt ctgatcatcg acgacatgat ctcttctgga gaaagtatga tcgacgtagc
    10861 caaagaatta aaacgcagaa atgcccgtcg tgtattctgc ttatctacat ttggtttatt
    10921 tacaaacgga ttagaagtat ttgaccgtgc agttgaagaa ggtattattg agaaggttat
    10981 cacaactaat cttacttatc agacacctga attattatct cgtgactggt atgcatcttc
    11041 tgacgtatcc aaatacgttg cactattaat tgatacatta aatcatgatg catcaatcag
    11101 tgatctgtta aacccaacag aacgtatcaa agaatatctt gaaaaaaaga atatgtaata
    11161 tatagataaa gacttcgtct tatggcggag tctttttttg cagaattttc ttatacacaa
    11221 aaatcacagg tattgaatta cctgtgattt ttaattatct caatattctt tttactgatt
    11281 ctctgctggc tgttcttctg tctgttgtgc tgccgttgta ggagccgctg ttgtaggcgc
    11341 tgccgttgta ggagctgctg tcgtaggtgc tgctgtagtt gcttctgtgc cctttgtgct
    11401 gctctctgtc gctgttgttg cctgtgttcc tgtctgctgt gtagtctctc cctgataatc
    11461 tgtataattt ccacccatag aatctgtaga agagtattct gaatgaatcg cattcatgaa
    11521 attactccag atggattttg gataagatgc tccacttaac gattccataa tcctgttatc
    11581 atctcttcct acccatactg ctgttgtata gtaaggtgtg tatccacaaa accatccatc
    11641 tttattcagg cttgttgtac ctgtcttacc ggcagtatca acattcggta ccttcgcacc
    11701 aacacccgtt ccacctgtga caacactctt cagtacatct gtcattgcat ttgctgcatt
    11761 tgtggaatat actcttctct tctttgtaga agatgacatc acttcatctc catcagaagt
    11821 tgtgatctta gaaatacacg ttggttctct atacacacca tcgttcttca atgtagcata
    11881 tcccgctgcc atctcttctg atgttgttcc tttcgtgaaa ccaccaatac atgtcgtata
    11941 atacttataa tcagaatctg ttagatattt aaaattcatc tcttccagat attttaaaca
    12001 ggtctttggt gtcaactgct catagatccg cattgttgca acgttacttg atttctgaac
    12061 ggctcttcga agtgagatct gtccagaata agaaccatga gagtttctta cacgattctt
    12121 atctttcgtt gacatcggcg aatcattcac cgttgatcct gcagaccatc catgttctaa
    12181 agctggtgca tatacaagca atggcttgat cgcactacct ggctgtcttg gactctggaa
    12241 cgcacggttt aatgtataac cgatattctt ctgttcacgt ccaccgacga tcgctacaac
    12301 ctttccagta tcattatcaa tacatgttcc agatgcctgt gtcttataaa tgccattctt
    12361 atctttctct gtatatccag acaatgcatt gtctacactg ctctgcagtt gtttctggac
    12421 atctggatca atggatgtat aaatatgata tcctgcactg tataaggtcc tcttacactc
    12481 tgcatacatc ttatcatatt cttcctgata tttttccttc tcagaagtcg aactgaaatc
    12541 atatttgaat tcaaatccct tctgtttcat aatctctttt gtcgcacaat gaatgacata
    12601 tgtttctacg taattcttct tctctgtctt actcttctgc gcactcttta cgttaatctc
    12661 ttcattgatc gcttcttcat actggtcttt acggatcagt ttctgctcgt acatatcctt
    12721 tagaatctta tttctacgct tgatcgtatt ttctttgttt gtacgtggat catagattgt
    12781 tggattattt ggaatcgcag ataaaaatgc tgcttctgac attgtcagat tcttcgcatc
    12841 cttacggaaa tatccttggc ttgctgcctg aattccataa taaccattcg cataataaac
    12901 attgttcaga tagaactcaa ggatctgctc cttggagtat ttcctctcta attctactgc
    12961 cataaagatc tctttaactt ttctcttcca tgtcttctga cgattcaaaa agacagtctt
    13021 cgcaagctgc tgcgtcagag tacttccccc ctgtgtgatc cttcctttgt gtaccacata
    13081 acttactgca gctcttgaga ttcctttgta atccacaccg ctgtgtttat agaaattctt
    13141 gtcttcgaca gaaattactg cagctatgac ataaaccgga agctccttgt acttcagata
    13201 atatacatct ttttcacctt taaacttcag taactgtttt ccgttggtgt catatacaat
    13261 acttgtctgt gatgccttaa atgtctcctc actcgactga gcaacgagtt tcttggcttc
    13321 acttctaagc ttcataacct ttgccccaaa agtaaaccag ccgattacga ccaacaacaa
    13381 aaagacaaca gaaactttca aaagaacttt tttcttttta tgtttctttt tatgcttttt
    13441 cttgctttcg ggaataatct tttttgtttc ctttgtttct tcagccatat tcctttcacc
    13501 tttcgtttgt ttttactgtt ctcattttcc ggatcatctc cagatatata tcttaatttt
    13561 aacataaaat atatcataac taaaattttt gtctcgacat agttcgcact ctaaagacaa
    13621 aatctaattg gcatatccgt aataaacgct tcctgcctgt ttaaattcac tctcaagctt
    13681 cttgcgattt gcttttacag actttccatt tgtcggatca taatatctta aatccgtctg
    13741 attataactc ataactaata catatgtgcc attctttctc tgtgccacga aagatcttcc
    13801 cttacttatt gcataaacaa gatcatccat gctgcagtca gaataattaa ctgcacgctt
    13861 attgaacact ttaaataatt tatcatatgt gctgcctgac atcttcttga tcttatcata
    13921 ggaaatctta attccttctg atcttgccat catcagaata catgcaatat ctgtctcatc
    13981 tttattcttt gctttgatca tcgggatatt agatcctttt ccataattct gtgcaacacc
    14041 tttctcccac agatacatct gatgataatt tacaacaact cctccgcctt gcttggcgac
    14101 cttgatagct tctttcagat tactgtaaat tcctgacaac ttaccgcctg tatagacaaa
    14161 tgcatcccta tatttattat catttacagc aaatttgatc tttatctcat cttcctgttc
    14221 ctcaacattt gtagaaagat aatttggtct tgtctgaata taaacatcat ccgggaatgc
    14281 aagatataac tgattcaatc ttgttcctgt atacccatac tctaaaactg ccttactctc
    14341 ctgtttatct ttgtaacgaa tataacttac attgatcttc ttatatccat ttccactctt
    14401 cttagaatgt gtcaatgcga tcgttgttcc gtctacactt gtgttcgtaa tataagtacc
    14461 atcttctcca tactctttta caatcttttt attgtttctt atatatacat ttgtcaatgg
    14521 atacatataa gaaccatctt tattccttac aacttctttt ttatttccaa ctccatagat
    14581 aaaatctttc tctatgaatc caattggacg cagtacctga tcatctttac atgtgatcac
    14641 ttctgttttc tgtgtggaaa gatccacaac tttcatctta ttctttgact gattcaatgc
    14701 aagcattcca ttctcagaga taactgtcat atcatctgta atgttctttg ccagaatctt
    14761 actctttcca gagtcaagat ctacctgata aaccgctcca tccaaataga aataaaactg
    14821 tccgctacca cttaaaaatg cacctttttt aatgtcattt ttcatcatat catactgaat
    14881 ctgtgttggg ataaagccta gttcctcaat cttctggtct gacgcagtat actgataaac
    14941 agaaatacca ttctctcctt catgttttcc tcgattcata tatccataaa ccaagaatgc
    15001 tatatctcca ttattcttca tcttcataat ctgaatcgca tgcttttgat agttgttgcg
    15061 actgtcacga taatcttctc cttcaaaaga aaatactttg tgcatgttgg acttctgata
    15121 gtcataatac cacaattcat cttggacaac gaatgctgct ttctttccgc catttttact
    15181 gatcaactgt ttctgactgc ttccgattcc caataaaata cgattattac tacgatcgat
    15241 cgtatcgtat cgcatatatg cttccatatt acgttcataa tttaacagat acattctgtc
    15301 ctttgcatat cgaaccctga aatattctgt tgcctgataa tactgttttg ttccatccgt
    15361 attctctgct gaaagaatat atctcagctc cagtgacgtt acattttcag aaatttcttt
    15421 tacggttgca ggatatacac tttcaacctg tggttttaat gttgcataag ataccgcttc
    15481 ctgtgtcgat ttcaagtcaa cattcgccag attattcgat gttgttccct ctgccgtttc
    15541 taaatactga ctgataaact ctgtattacc ttctgtcaat gctgcatcat ggaatttctg
    15601 tgcaaaatcc agacattctt ttaaatgaaa actagttcca taaatcacac gtgtaaaata
    15661 acggatcgtt ttgtcatttt gtttcactga caattccatt agatatgttt ttccattttc
    15721 cagacgttct ttgatcttaa tgtttgccgt cttttttcct ttgactttat gaaatgctgt
    15781 atcaccattt ccaaggattg ttttattatc tgtatcataa atcttatatg acacgacact
    15841 gacgtcagat tttgcatatg tcagcattac ttttacttca ttctcataaa ttggtgtgat
    15901 actgtcacgg atcaaggatg catcaacact tccctgatac ccatgcaact ggttaatatt
    15961 ctttccatct gcagacactg ttaataaagg gattgttgct tgactagttt ctgtaacccc
    16021 tttgttattt cttccaacta acatataatt ccagataaat aatgaaatta aaaatagaat
    16081 tccaaatact gaaatttgta tcactttact ttgcttcatt actttctcct tttcctaatt
    16141 ccgtgaagcc ttataatgta cattttatcc gtttttgtat atgaatacaa cctttttaag
    16201 aaatgtttaa gatgtatgtt tttcatttta cagcggattt catagtgatt ccctatgaaa
    16261 caagaaaaaa ccaggatttc tcccggctct ttctttttat gctttaaaca tatttcgttt
    16321 tcttcgataa cggcatcttc tatgataaat ctctcctcca agcagcgaaa ggatcatctg
    16381 atataataat tgttctttct tctgacaatc ttgcttctct tgatgatcga tcttatcata
    16441 aatcttatcc accatagcaa gcagcatcgt tcgatccata tattcatcaa acatcctgct
    16501 gccctcgtat tccatctgat cacaaatatc ttcgatcaga tccagaatct gtctggccag
    16561 tgcagaatac attctcgtaa tatactgata atcgtcttcc tgcctctgtt cctgtgctac
    16621 aaattctctg actggtctta cttcagtatc aaataatgga agatctccta aaccatctct
    16681 gtctgccatc ttcctgctcc tttttctcta tcaatcatat cttatgatca tttagagaaa
    16741 atattgcatg tcctattctt tattttcttc ccgaatactt ctcacttctt cattgatcgc
    16801 caacatcttg ctgtctaaaa acgtcacaag atctgcacac tccttcaatt ctttcttaat
    16861 gtattctggt gtattgccct cgtatttata ataaatctta tgctccagag atgcccaaaa
    16921 gtccattgca accgttctga tctgaatctc tactttggta ggtaccactc tgtcagataa
    16981 aaagattggt actcttacga tcagatgata gcttctgtat ccattgacct tgggattcaa
    17041 catataatcc tttgtcttta gtacttctat atcatcttgt gatgagatca tatctacgat
    17101 tcgataaatg tctggtgtaa aagaacaaat aaccctgacc ccagcgatat cgtcaatata
    17161 tttctggata ttctctactg tgatctcttt tcctttacgt atcagctttc ttgtgatgct
    17221 ctcatctgtt tttaatcttg attcgatatg ttcaatcgga ttgtactggt gcacaagttt
    17281 aaactcatca tttaatatct ctaatttcgt tccgatctct tttaatgcag aacgatataa
    17341 taatttgatc tttcggtatt caagcattac atcttgtaca tcaggtaatt ctaattctct
    17401 catatgtctc actccttacc atgtttgcta ttcctattct atcactgttt cactttgtat
    17461 tcaatagcat acaatccgag tagtaagttc ttaaatacct aaaaatgggt aaaaaaaatc
    17521 cgtgcggaat gcgttgtgct atcttccaca gacgttacct ctatagttaa ctcaacccag
    17581 gcaccctcac ggcacataga aggttccact tagtgctgct ttgttcccaa cctgacacgg
    17641 ttcatggatt cctattgcgt gagacccaga tatcaacgcc acttgtagag ggcagctcta
    17701 cagaaaacag cccgaagcaa accatcacct ctgctatagc ggattgcaga aatagggtgc
    17761 cgctacctcc ccggctagca cggaagcttt atgacttgat tatctttggc atcttttgca
    17821 agatgcatat ttgattatac taaatacttt tcttttggtc aatagttatt cttatttggt
    17881 ttttagataa atttttttcg ttccccgata tcttcctctt ccttttacga tcacatattt
    17941 gtgaccttct tttttgacga tcttgtaatc tttattcttt tttaaatgct ttcctttgta
    18001 atataatacg attccctttg gattcttatt tttagaatcc gccttacaat atctcaaatg
    18061 aatcttgttt actggctgca ttactgtata tgcctttaaa cgcatcgttg catgatctgg
    18121 gttcagatca ttccattttc tatgatcata aagataactc tgtccttttt ttgttttatt
    18181 aacaaaagat acccagtgat aatcatcatt ttcatcaaaa ccaatcttac cagaagatct
    18241 taattttact acaactgcaa acttgtctcc tctcagaacc tctgcttttc ttgaaagatc
    18301 gatcgtatgc gttccggcct ctctgatcgt tcctgttacc ggacgtttta acatctttgt
    18361 tccacttgtt ggtcttccat ctttaggatt tctataaatc tgtatactgt atttgacgtt
    18421 tgcagaccaa gttaccagat tcactgcttt gatcagttct ttcttctttg cacttccttt
    18481 tttcgctgta aatacatttg cgaacttttg tccttttttc agttttaaaa ttccaggatt
    18541 cgctgaacca tcatagaaat aattgtattt atactctgag gctttcacaa attgccctgc
    18601 aacaaattca caaagagatt tatcttcata agaaatatag gtatatccgt tacttccttc
    18661 ttgatttccc caactgtttt ttacgatcca tgctccatct ctggacggtc tggatccact
    18721 gttaaaatta tctctgctat aactatcatc ccatccaacg atcgcaacaa tatgatttgc
    18781 tgtctgtctg gcactattgc agttgtatgc ctcatgattc tttccgtaat aggctgtttc
    18841 ttcccatgtc tcttcgggtg catggtacgc cataactacg gaaccatatc ctataatata
    18901 ggattttact actgctgctt gcggtgtatc tccaaggaaa aatccattct tcagaattgc
    18961 actgtctttt tgtcctacag aatcagataa tctccatttt cctgtattaa atggtgcaat
    19021 attttctttt gcaagaccat accatcctgt tagcgacaac attgcaagca gtgaattgcc
    19081 acccgcatat ctccaatctc ctgaaacttt tgttttgtcc cctttggtat tatttaatat
    19141 atcgtttttt cgattgtaca taaaatatcc aaaatgtttc tccgaaaaat ctatattcgt
    19201 acttgcgatt ccttgtttga tcagagaagt ctctcccatc gatgtaatcc caaacgccca
    19261 gcataattct gtgttcccct gatctttcac tgatgttacc tgtccaagtt ctgatgaact
    19321 gtaatatgaa tccaatgatc tggcattcct ctttggcgtt acagttttgg gcacatctgg
    19381 agattcaatc ttaatattca atggatgata tccattagac tgtgaggcat agataaaaga
    19441 aatatttaaa gaacttatca tgccgacaca taaaataact acaataagct tcttaatcct
    19501 ttccatgttc cttgtccccc ttcttccttc ttcttaattc tttaaaaaaa tcagacagca
    19561 tctgactgca ctcttcctct aatataccac gttctatctc tacttggtga ttaaatttgt
    19621 caatctgtaa aatattcaat acagatcctg cacagcccgc ctttggattc atcgctccga
    19681 tcacaacttt gggaattcgt gcctgtacga tagctcctgc gcacatctgg catggttcca
    19741 atgtgatata catggtacaa tcttccagtc tccaatcatt aactttctta cttgctttct
    19801 gaatcgcaag aagttctgca tgtgccaatg ttgtcttctt cttattgcgc tggttataag
    19861 ctcttgcaat gatcttatcg tcttcaacga tcacacatcc aattggaaca tctccgatca
    19921 ctgccgcttt ctttgcttgt ttgattgctt ctttcatgta tttttcttct tgtgtcactt
    19981 actcttaccc cttatatcta tgtcatattt ttctttatta taacatagaa tgaagcaaaa
    20041 agagagtaca cgattgtcag attatcaacg tattgtatct tatctgtatg aatatcacca
    20101 tggaatcaag ggttccaatg tcggttttgc caaaatagaa aaacgccaaa accgtttgag
    20161 ggtctatttc catgtaaaaa caaatgaaca ggcattagaa tacaaaattt atttttatta
    20221 ttttcatcag ggtgctatga atgggattta tcttgatacg gtcaagcgaa atgatgcgat
    20281 cattgaattt aaaaacacat atcctttgat cgatgatttt gataaaatgg atggattctt
    20341 gctgtattat tcgaatgatc atttctttgg atcgcagtgg aaagatcatc cgatcattat
    20401 acgaagtttt gtacctgtgg agtctgctga aaataaaaat gagaatactg aaaaaacggt
    20461 acctccagaa ccttcacctg aaaccgaaac actttctgaa actgaagctt ctcctacatc
    20521 cagtctctca gaatacattg aagcccttca attagaaaag gatgcgaaag aattcacaca
    20581 tccaaatcca ccaaaggact tcgactggaa ggagtatccc accttgccca tgcctccttc
    20641 ttatagcctt gatcctagca ttaagatcgg actagaagat cttgatcaga ttcctgatct
    20701 tgatgacaac ataaagacaa atggttttct ccttttaaat tatgggaatt tcggccactt
    20761 aatgctcgcg aaagatcaag acagcggcca acgttatctt ggaattcctg gaatctttga
    20821 taacgaaaaa gcttttatct cccgtttatt tggttttaag acttttatca cggtgccgct
    20881 aaaaccacat aaaactggaa attttggtta ttttatctta ccggtctaag atgttactta
    20941 actgtgcaaa ctgttctaag gttaatgctt ctccacgaac agatgcaggt aattccatct
    21001 cttctaatgc ctgtgctacc tgttctttgg agaagtttaa gcctccgaaa tttttaattc
    21061 cattctggag agtttttctt ctctggttaa aagatgcacg aattaactgg aataataatt
    21121 tctcatttgt aaccttgatc ggtgcgtctt tgtatttcgt aagacggatg actgcagatc
    21181 caacttttgg tcttggcata aagcagtttg gcggcacatt cgctaccaca tatggttctg
    21241 catagtactg cactgcgagt gataatgcac catagtcttt tgttcccgga cctgactgca
    21301 tacggtctgc aacttctttc tgtaccatga cagtaatact gtcgatcggc acatgacttt
    21361 caaacaatcc catgatgatc ggggttgtaa tgtaatatgg gagattcgcc acaaccttca
    21421 ctggtttccc accatttttc tcttctacga tctttgcaat atcgactttc aaaatatctg
    21481 cattcaaaat ggacacgtta tcatatgcac ttaatgtgtc tgaaagaatc ggaatcagat
    21541 tcttatcaat ctcaactgcc atgacctctc tcgcattttc tgcaagatac tgtgtcattg
    21601 ttccaattcc tggtccgatc tctaacacga aatcatcttt gttcacttct gctgcagaaa
    21661 taatcttctc aagaacacgt tcatcaatca gaaagttctg tccaaacttt ttctgaaaat
    21721 caaaatcata tttctgaatt acttctattg tattttttgg atttccaagt ttcactcttt
    21781 atacctcttt catacgatac aattgtcttg cattttcttc agtgactgcg atcagttctt
    21841 ccttgttgat ccctttgatc tgactgagcg cttctgctac ataatttaaa tttagtgaag
    21901 aatttctctt gcctctattg ggttctggtg acagatatgg gcagtctgtc tctaatacga
    21961 ttctgtctaa cggtgtataa gctgcaactt cctttaatgt ctttgcattc ttaaatgtta
    22021 caacgccacc gattccaata taaaatccca tatcaaggaa ttgccttgcc atctcttttg
    22081 aataagaaaa acagtgaacc accccgccga tttcttctgc atgtaacgct ttcattatat
    22141 cataagtatc ctttgctgcg tccctgctat gaataatgat cggtaatttt acatctcttg
    22201 caagttcgat ctgtgcttca aaccatttct tctgaatgct gcgttctggt tcatcccagt
    22261 aataatcaag cccgatctct ccgatcgcaa gaattcgatc cattgctgca tatttcttta
    22321 gtgtatcgat atctgactct ttcaactctg ccgtatcact tggatgaacg ccgactgctc
    22381 catacatcat cggatatcgt gtcactgctt ctacgatcca tgtagacgtt tccatattcg
    22441 atcctacatc aacgatcgtt ccgattccat tgtccttcat agatgctaac aattcttctc
    22501 tgtcttgatc aaactgtttg tcatcataat gtgcatgtgt atcaaatatc atcgtctctc
    22561 tcccttttct cttcgtttct ttaatctgac tgtatttccg ttgaccgtaa gacaacggat
    22621 gcttcgtaag aaacttgaca tacggctgta accatagtct ttgatattaa atccgctgtg
    22681 tttatctttt aattctaacg ttaccgcaga aagattatct aaagatccac catttttctt
    22741 tacaaaatga attacttctt cttcaatcgt ttctctgctt gtcagttctt taaattcaac
    22801 ataacagttt gtatcttcct gcacaagcat aaaattatcc atctcttcat caattaaaac
    22861 actcagcttt gtatatccat aatttcgcac atcaaaatct gaatattttg catttaatcg
    22921 atttccaatc ttccccaaat ctactggtgc atttccattt tcactgataa tatctgcgat
    22981 cgtatatgaa atctcatcca gtgatgtaac attctctatt tcatcacttt ccatatcctc
    23041 ttctttacct ctgtccgctt cataaatcag atttagatgg ataaatttat tacatgctct
    23101 tgtcagtgcc acaggtgtct ttgattctcc cattccgatc acatacatat gttcttctcg
    23161 aagtctcatt gcaagtcttg taaaatcact gtcactcgta acaatgcaga atccgtctac
    23221 tttctttcca tataacagat ccattgcatc aatgaccatt gccatgtctg ttgcattttt
    23281 acctgaagta taagaaaatt gttgcacagg aataatagaa tattctaata acaactcttc
    23341 attccatcca tttgctttgg accagttccc ataaattctt cgacaagacg gaaatccgta
    23401 ggcttccagc tcctgaaaaa tgatcggtgc gtatttagga ctgatattat ctgcatcgat
    23461 cagaactgca aactgattct tttcttcttc tatcatattt tccctcatat ctagctattg
    23521 tttatagttc tttcctgtaa aatgaaaaaa aggatatatc ctctcgaata tatccttaaa
    23581 taaataccaa gcgggtgatg ggaatcgaac ccacgtgatc agcttggaag gctggagttc
    23641 taccattgaa ctacacccgc atatcttgtt cgctgtgttg tgttttcctc agcgacaagt
    23701 aatactatac caccactttt tataaattgc aagtgttttt ttgaattttt ttcatttttt
    23761 tgaatatctt atgataaagg ggttttgggg tatattttat gatgaataag atctggtgtt
    23821 ttatgatcct tgcaggactc attgtcagca gttttaatgg aaagatcaca acactttctg
    23881 ataccattct ttcttcttcc aaagaggctg tggaactctg tattctaatg tttggaattg
    23941 ttggattatg gtctggactc atgaacattg ctctgtctct tggaattact acacaacttc
    24001 aaaagctgtt aacccctttt ctgacttttt tatttccaaa tctgaagaat caaaaagcga
    24061 aagaatatat cagtacaaat attgtcgcaa atatattagg tcttggttgg gctgcaactc
    24121 cttccggatt aaaagctatg gaagaattac aaaaagataa ccctgacaaa aacaccgcaa
    24181 caaatgaaat gtgttcgttc ctaattctta atatatcatc tctgcaattg attccgatca
    24241 atatcattgc ctaccgaagc caatacggct ctgtcaatcc atctaagatc ttaattccag
    24301 gacttatcgc tactttaggt tccacgatcg ttgctgttat ctatatcaaa tggaaggacc
    24361 gaaaacgatg atcacatttc ttgcaaatct tgtcattcct ttattcatat ttacacttgt
    24421 tttatatgga accctaaagc gtaaggatat ctatacccct tttcttgaag gtgtcatgga
    24481 tggatttaaa attgttcttg agattgcccc aactctgatc gctttgtttt ttgcgattca
    24541 gatctttcgt tcttctggag ccttggatct gatcgttcgt tttctgactc ctatgggaaa
    24601 actattaaag attcctaaag aagttttacc cgtgatcttt gcaaaacttt tttcttcttc
    24661 cgctgcaact ggattcctgc ttgatattta taaaacttcc gggccggatt ctttggctgg
    24721 gtttatgtct tctgtgatcc ttagttcaac agaaacttgt ttttatacat tatccgtgta
    24781 ttattctgtg gttggaatag aaaagattcg atatacattg actggggcat tgcttgccgt
    24841 ttttgttggt acttttattt ctgtattcat ctcttatttt taacttttcc cgatatattt
    24901 atgatataat tttgacataa ataaatttag attgcattat aaaaatcagc atacacaaaa
    24961 aacgagtgtt atcagcatct actactgata gcactcatct tttttattcc atatcttttc
    25021 gtttctctaa ttctctcaaa ctgacaacca gatcataaac ttccggccgt cgttctttca
    25081 aatgttcatc caaacgatgt cttcgctctt taaatctctg tgcaagttct ggatgttctt
    25141 tcataatacg ttcatgaatt tcccttcgat gttcctcaaa acgtgccgcc agagttcttt
    25201 catcttttcc agaaatattt acgatctctt ttagatgttc ttccagatgt tctaaccatt
    25261 catcttcgtc aaaagcttcc atatgtgacc agcttccatg caataaattc tccacatctc
    25321 cattttgctt taactgctta atctgttgtg caatctgcct ctcaatctga tcttccgtgt
    25381 cagaaccaaa tgcccagaca aattcctgca tacggttatc tacacgcttt cttgatgcac
    25441 tgcgttcttc attatcaact ccgccttcta atggatacgg acgtttgatc cccatatctt
    25501 tcaacgcaat ataaatcgtc cttctgacac atccttcttc gatcagatat ccgattccaa
    25561 gctttctcat ctgctcattt aaatctgcga tatgctctgt gatataaaat cttattcctt
    25621 gctctttcaa tgacttataa agaatctcaa tgcggtctgc tgccgtgata tccatacttc
    25681 caattccgct ggcatcaagg atcactgctt tgatatttcc tctgcgttca agttcctgct
    25741 caatatcctg ctgcaacact cctgcattgg caaaaaacaa attgctgctg aaacgataga
    25801 tcaacacacc ttcaatctcg tgaatctgac gactctcagc taaatcacga aaatgcttat
    25861 gtcctggctg aattcccaga aaacaacttg ctggttttga tgatcgtatc accatttctg
    25921 caaaagataa gataattccg atcaatacac cattgatcgt tccaagtaac aataccccaa
    25981 taaatgcacc ccagaaaata aaacattctg tgcggctgac tttccacagt tttaccgcca
    26041 gttcaaattc tgttgctccc agaagtgcgg aaattacgat cgccgtcaat accggaactg
    26101 gcagatatcc aataaatcct gttccaaata acagtaatag gatcatagat aatcctgcaa
    26161 caatactcat taactgtgtt tttccctgat attgttctcc catcgccgtt cttgaaacag
    26221 atccgttgat cggacagcat ccagtcaatg ctgccacaag atttccaact gcaaatgcta
    26281 gtaattcttg attatcattg atacgatatc catttttctg tgcaaaatta ttctctgcaa
    26341 gaagtgtttc cgccataata acgaccgcta ccgacaaact gatcgtcacc acttcatcca
    26401 ttggaattaa cataagattt ggaaaactcc attttggaag tcctggtgct acggcatcta
    26461 atgtcttaat tccccattct ttcattggca gaacctgcgt caaaacagcc cctgctgcca
    26521 tcatcacaac tgccattggg aatttcggca tgatcttttt cgctgccaac aagacgacta
    26581 atgataggat tcctaaaatc aatgatggaa gattgatctg ttctgctgtt ttttctatat
    26641 gctctaacag ttctaataat tctccagttc ctgctgttcc tcccattaat ttcggcactt
    26701 gcatcaaaat gatcgtcgaa cagattcctg taataaatcc acccataact ggtgctgaaa
    26761 tataatttac aagttttcct gcatgcatca gataaaatgc gagcagccac aatgccacaa
    26821 aaaatgtgat cactggcaca actgcaagtg cttctttgga accactttgt atattcatcc
    26881 caagaagtgc tgatccaacc aacgctgctg gtgccgcatc cactccaaag atgaactgcg
    26941 gagatgtaga aaataatgca aataaaatga tcggaaatac agacccatat agcccataaa
    27001 ccgcaggtaa tccggcaatc tgggaatatc ccatagaaat tggaatcgat actgccatga
    27061 tgatgattcc tgtgatcaca tcatgcaaca gattttctct tttataatct tttattgtat
    27121 gtcctaatat cataattctg tatgaaacac tcccaacaaa ctattctctg atttccatcc
    27181 aagatatctc aatgtttctt ccaatccaga catcacttct aacatatctc ctacatttgc
    27241 acttgcaccc atatgcccga ttcgaatgac ttttcctgcc aattctccga aagaacctgc
    27301 cagcatgatt ccatgttttt ctctcattct ttccagaata tcatccgctg tcattccttc
    27361 tggtacatca aatactgtaa ccgtatctga aaatccactt tccaaatgta attttagacc
    27421 aaatgcttca atggcttttc ttgtcgcatt ggcgattctg gcgtgacgtt tgatcagatc
    27481 tttatccgct gcaatattat ccatcgctgc tttcaatcca tagatatcac tgatcggcat
    27541 tgtatatggg aaccataaat ttttatagta atctttgaaa atcgtcagat tacaataaaa
    27601 tgatgcgatt ggtgtttttc gattttccat agctttttta gcttcatcac tgatcgttac
    27661 gaaagtaagt cctggaggcg ctgaaactgc tttttgagat cctccacata agatatcgat
    27721 ctgtgcttta tcaacattga cttcatttcc aaacattcct gaaacagaat ctgtaactgt
    27781 catgattcca taggatttca ataatggaca gattttatga atgtcattta acattccact
    27841 tggagtatca caatgaacaa ctgttgcata tttaaaatcg tgatcttcct tcagatatgc
    27901 atctaattca tcaacatcaa atgcattttt ataatctttt gtatacataa caggagttcc
    27961 accatacata gatacaaaat ctgcaaatcc tgctccataa attccattat caagaattaa
    28021 aactctatct cccggctctg tcatagatgc acatgctgcc tctaaaccaa ggattccttc
    28081 tccgcttagg atcagtgttt cattcttcgt atgaagcagc tcactgatca gctcacaggt
    28141 ttccttgtat tcatcataga attgcggatc caaatctgca ttcgttgtag aaaggcttct
    28201 cgccagtctt acattttctg gcacctgaac aggtcctggt gtcataatct tgtatttact
    28261 catatccgtc actcactttc ttttttattt ttataatcta atataccggg atcactgcat
    28321 agaaaatgct ttggcaatcc cggtatcatt atatcatttt attatgcgaa ctgtgtaatt
    28381 ccaattttct taactgcaat ctcaattggt tcgatcacga tcagtacaac aactgctgct
    28441 acagcaatct gtacgacatt tcctggaaca gaactgattg gtgcaataaa gtttccataa
    28501 agaatgatct ctgcaatata atatccaacg atcttaatta caaatgctgc aaagattgct
    28561 gcaactttcc atccatactg gtcatgcttc tctgtcataa ctccaacaac atatcccatg
    28621 attcctacga taataaatgt aaatggtgcc cacgctgtcc atcctgacaa cagatcaaat
    28681 aatcccattc caactccacc agcgatagct cctgttttct taccgaataa aattgccata
    28741 ataaatagag gaacatttcc taaatgaatc agacctccat tagctgcgat cggtaatctt
    28801 acgttaacaa acgctgtaaa cacataagtt aatgcaactg ccattgctgt tattgcaata
    28861 aactgtactt tattttcttt cattttattt tcctcacttc ctgatttctt gtatgatata
    28921 tagtttttca agtacgctgc gttcctttat tgtgttataa tttatcgtgt tattggtatt
    28981 tttaaaatag ccagtttttt tatttttaat ggtgccagtt tatgat
//
LOCUS       DAOMUE010000005        23879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_318_length_23879_cov_3.563214, whole genome shotgun sequence.
ACCESSION   DAOMUE010000005 DAOMUE010000000
VERSION     DAOMUE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 23879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23879
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_318_length_23879_cov_3.563214"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1287)
                     /gene="serS"
                     /locus_tag="PLC71_00660"
     CDS             complement(1..1287)
                     /gene="serS"
                     /locus_tag="PLC71_00660"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRM01189.1"
                     /translation="MLDIKFLRENPDVVKQNIKNKFQDRKLPLVDEVIELDKENREIK
                     QEVQALRADRNKLSKQIGALMGQGKKEEAEEVKKQVTASADRIEELSEREKVVEEKIK
                     EIMMTIPNIIDPSVPIGKDDSENVELERFGEPVVPDFEVPYHTEIMESFDGIDLDSAR
                     KVAGNGFYYLMGDIARLHSAVIAYARDFMIDRGFTYCVPPFMIRSNVVTGVMSFDEMD
                     AMMYKIEGEDLYLIGTSEHSMIGKFIDTTTEEAELPKTLTSYSPCFRKEKGAHGIEER
                     GVYRIHQFEKQEMIVVCKPEESKDWYEKLWKNTVDLFRSMDIPVRTLECCSGDLADLK
                     VKSVDVEAWSPRQKKYFEVGSCSNLGDAQARRLRIRIKGENGNYFAHTLNNTVVAPPR
                     MLIAFLENNLREDGSVAIPEVLRPYMGGKESLQPKK"
     gene            complement(1300..1791)
                     /locus_tag="PLC71_00665"
     CDS             complement(1300..1791)
                     /locus_tag="PLC71_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HRM01190.1"
                     /translation="MTDFFNSVGMEYVVIGLAAALLLALILVIVALVKVGKLKKRYEK
                     FLSGKDGKSLEESIVKRFDKLNEIIVEQDFLEQEIQRIDEEHKSSFSKMEVYKYNAFS
                     DMGGETSFVIALLDHNNNGTLLNGMSSQNATYVYAKEIRNGASKVALSNEEKLTLEKC
                     INK"
     gene            complement(1804..2724)
                     /locus_tag="PLC71_00670"
     CDS             complement(1804..2724)
                     /locus_tag="PLC71_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRM01191.1"
                     /translation="MSAKKMGLGRGLNTLIPKAPIVDEEDVEQVKKKVSRSSKTKKKE
                     ETKKELTLPIDKIEPNPDQPRNQFDEDTLQELADSIKQYGMLQPILVTPKDDFYEIIA
                     GERRWRAAKQAGLNEVPVMIRKYNENEIVEIALIENIQRDNLNPIEEAMAYKRLMEEF
                     ELKQDEVATKVSKSRASITNSLRLLKLDPRVQKMLEEEMISTGHARALLAISNKDKQY
                     EIAQKVFDEKLSVRDIEKLVKDLKKIKKNKKEEKDVHDFLYTELEESMKQILGSKVSI
                     KNKKNNKGKIEIEYYSRDELERIVDMIRSI"
     gene            complement(2724..3494)
                     /locus_tag="PLC71_00675"
     CDS             complement(2724..3494)
                     /locus_tag="PLC71_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM01192.1"
                     /translation="MGRIIAIANQKGGVGKTTTAVNLSAALAARGKKTLLVDMDPQGN
                     TTSGLGLEKNELDKTTYEVITGEEKIQNCIVKDLFDNLSLLPANRNLAGAEIELMTVD
                     KMQFIMKHNLEKVKKQYDFIIIDCPPALGMLTVNAMTAANTVIVPIQCEFYALDGLTQ
                     LIYTIELIQKSLNPDLTIEGVVFTMYDARTNLSLQVVENVKSYLNQNIYKTIIPRNVR
                     LAEAPSHGLPINLYDPRSSGAESYDLLAEEVIENGGEN"
     gene            complement(3779..5464)
                     /gene="argS"
                     /locus_tag="PLC71_00680"
     CDS             complement(3779..5464)
                     /gene="argS"
                     /locus_tag="PLC71_00680"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRM01193.1"
                     /translation="MRNKVIDLLDQTIEELDKETIDGILEIPPKDDMGDFAFPCFQLA
                     KVFRKAPNMIATEIAEKIPASEDVSKVEAMGPYVNFFLNKENYVKEVMAKVNGTYGAQ
                     EIGKGKTVCIDYSSPNIAKNFHVGHLRTTIIGNSLYHIFDKLGYKVERINHLGDWGTQ
                     FGKLIVAYKKWGSKEAVEEKGIPELLRIYVKFHEEAEKDDTLNDQAREWFAKMEQGDE
                     EALAIWEWFKDISMVEYKRIYKMLNMDFDHYTGESFYRDKTAAVVEELKEKNLLKESE
                     GAMIVDLDEYDMAPCLVMKKDGSSIYATRDLAAIFYRKKEYNFDKCIYVTGLEQKLHF
                     AQVFKVVELMGYEWAKENLIHVPYGLVSLEGGKLSTRSGNIVYAEDILKESVSKIREI
                     IAEKNPDLKDKEDVAQKVGIGAIIFNDLYNQRIKDVTFTWEKIHSFDGETGPYVQYTY
                     ARAASVLRKTGITEVGEIDPSLVTDETSVALLKEIERFPEVIKVAADRLEPSVISRYV
                     MGVAQSFNRFYHENQCNVEDQKLKEARVKIVILAKQVIKDGLDLLGIQCPEQM"
     gene            complement(5477..6229)
                     /locus_tag="PLC71_00685"
     CDS             complement(5477..6229)
                     /locus_tag="PLC71_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01194.1"
                     /translation="MEKNKDKSHLIDQLYDYLEEQIQELKTDRNQLNHKMQDMIQEKN
                     EKEGAINNQQKIYQIRKVFSPLPLEIEEEKQPVSEDMIQIEKEIHEFQMILREKEEKI
                     TKLHDYQKGLEETCLFAEEFVEREDQKILFVPAFEQLMGYVKKLYPTQRINYQDSSES
                     SKVCMTFSFLKGFQAVLESLLNEIGVFVLNFEMNIDEYKILINIEAKPKVIKNAKDFY
                     EKRSVLENKLTKEFSIERWKTNAVVIQTMIEM"
     gene            complement(6289..6999)
                     /gene="rsmG"
                     /locus_tag="PLC71_00690"
     CDS             complement(6289..6999)
                     /gene="rsmG"
                     /locus_tag="PLC71_00690"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRM01195.1"
                     /translation="MERLREKAAAFGIKLSDHQLEQFETYYEMLVEKNKVMNLTAITE
                     KNEVIDKHFADSLALIKSGVSLTGQKILDIGTGAGFPGIPLKIAFPELEIVLLDSLNK
                     RIKFLNEVIEALGLEKITAIHGRAEDFAKQKEYREQFDYVVSRAVANLTVLSEYCLPY
                     VKEDGYFLPYKSGDIKEEAANSKKAVKILGGSIEDIISFEIPDTDMARTILKIHKTKA
                     TPKRFPRKAGLPTKEPIC"
     gene            complement(7003..8886)
                     /gene="mnmG"
                     /locus_tag="PLC71_00695"
     CDS             complement(7003..8886)
                     /gene="mnmG"
                     /locus_tag="PLC71_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRM01196.1"
                     /translation="MNNLEEKYDIIVVGAGHAGCEAALAAARMGFETICFTVSMDSVA
                     LMPCNPNIGGSSKGHLVKEIDALGGEMGKNIDKTYLQSKMLNKSKGPAVHSLRAQADK
                     KMYSISMTQVMGNTPNLTLRQGEVTEILVEDGKIKGVKTYSGAVYYAKAVVLTTGTYL
                     KARCIYGEVSNHTGPNGLQAANYLTDSLKSHGISMRRFKTGTPARIDKNTIDFSKMAE
                     QFGDERIVPFSFTNKEEDIKRDQISCWLTYTTEETHEIIKENIHRSPLFSGAIEGTGP
                     RYCPSIEDKIVKFPDKNRHQVFIEPEGEYTNEMYVGGMSSSLPEDVQYKMYHSVPGLE
                     NAKIVRNAYAIEYDCIDATQLKASLEFKEIDGLFSGGQFNGSSGYEEAAAQGLMAGIN
                     AARKLQEKDPIILDRSQAYIGVLIDDLVTKETQEPYRMMTSRAEYRLLLRQDNADLRL
                     TKIGYEAGLISQERYDKLLVKEQQIEDEMKRLEDINVGASKNVQSLLEELGSTTLKSS
                     AKMTELIRRPELTYMDLAPIDPERPEYDLDVQEQVNINIKYEGYIKRQLSQVKQFKKM
                     EKKRIPEDIDYEDVGSLRIEAKQKLSKIRPSSIGQASRISGVSPADISVLLIYLEQMH
                     HRR"
     gene            complement(8889..10265)
                     /gene="mnmE"
                     /locus_tag="PLC71_00700"
     CDS             complement(8889..10265)
                     /gene="mnmE"
                     /locus_tag="PLC71_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRM01197.1"
                     /translation="MKSNTIAAIATPMTNSGIGIIRISGDEALDIIEKVFKPKKKDKK
                     IKDVKTYTAHYGHVYDESTLLDECIVLVMKGPHSYTAEDVVEINCHGGVVVMKKVLEA
                     VFKAGATPAEPGEFTKRAFLNGRIDLSEAEAVMDLIQSKNEFAMSTSLKQLEGALGKK
                     ITEIRKQIIHSVAFIESALDDPEHYSVDGFSDELKDQVEVIINQLNEFLENADNGRIL
                     KEGIQTVIVGKPNAGKSSVLNVLLGEERAIVTDIAGTTRDTLEESIQIKGIPLNIIDT
                     AGIRDTNDLIEKIGVDKAKDLLTKADLVLYVVDTSDPLTKDDEEIMELIEDKQTIVLL
                     NKADLDQVVKVSDLKEKGFEQIVQISAKEQTGIEELYQLIQDIFFEGHVSFNDEIYLT
                     NMRHKTEVSEALKSLAMVLQSIEDGMPEDFFSIDLLDAYEHLGFITGESVGEDLVNEI
                     FAEFCMGK"
     gene            complement(10314..11120)
                     /gene="jag"
                     /locus_tag="PLC71_00705"
     CDS             complement(10314..11120)
                     /gene="jag"
                     /locus_tag="PLC71_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HRM01198.1"
                     /translation="MSAKRFTGKTESDAVMNAAMELGIPSTDLKYTVIDSGSNGFLGL
                     FKKPVIIEIKEEEKETEVMKSQTIEKEQPKKKAEKKPSKKVDEKSNRQPKKSPKVKDE
                     VPVKEPRKNKTPENIDQIVKDTEKYLDEVLRAMGLDPKLNLYYNRRDNVLNINVSGEK
                     MGALIGKHGQTLDALQYLISLYVNKESDSFVKIKLDTENYRERRQQTLEKLAGSIAYK
                     VKKNKKPIYLEPMNPNERRIIHSALQRDPEVITKSEGKDPYRKVVVMLKK"
     gene            complement(11123..12124)
                     /locus_tag="PLC71_00710"
     CDS             complement(11123..12124)
                     /locus_tag="PLC71_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRM01199.1"
                     /translation="MMLLAQAQQSTGNGGILAPFVIIFGWIIRAIYDGLAAMGIYNVG
                     LCIILFTLVSKLILLPVTMKQQRSMKINQHMQPEINKITKKYRNKRDQASMMKQQEEM
                     QKVYAKYGSSPTGGCLPTLIQFPIIMALYYVIRGVNTYIPQIARDVQFKPNLFLGMDL
                     NSAPGFRLTPLLIIPVLSFIFQFLSAKTSMSTTQMDDSTPGAGMTKSMMYTMPLMSFV
                     MCISLPIGIGLYWSASALFQYIQQVAFNYHYDHADMDKIIEKSREKAAKKKKKKGPSL
                     YEKMLGMQAEQQGQQGADSNIKKSANLKSTQNIEYSGNSKGGIASKANALNKNKGGK"
     gene            complement(12121..12363)
                     /gene="yidD"
                     /locus_tag="PLC71_00715"
     CDS             complement(12121..12363)
                     /gene="yidD"
                     /locus_tag="PLC71_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRM01200.1"
                     /translation="MKKILISIIRFYRKYLSRLKGRGTCIYVPTCSEYAIEALEKHGV
                     VKGSLLAIFRILRCNPFSKGGYDPVPEVRKGKRCKK"
     gene            complement(12370..12708)
                     /gene="rnpA"
                     /locus_tag="PLC71_00720"
     CDS             complement(12370..12708)
                     /gene="rnpA"
                     /locus_tag="PLC71_00720"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRM01201.1"
                     /translation="MKNYHSLRNNREFQMVYNEGNSKANRYLVLYYRKNDLEYNRLGI
                     SVSKKVGNSVVRHRTTRLIRESYRLNQDNLKTGYDMVVIARQTAKGQNYHTIESAFLH
                     LIDLHHLKKS"
     gene            complement(12758..12892)
                     /gene="rpmH"
                     /locus_tag="PLC71_00725"
     CDS             complement(12758..12892)
                     /gene="rpmH"
                     /locus_tag="PLC71_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRM01202.1"
                     /translation="MKMTFQPKKRQRSKVHGFRKRMSTANGRKVLKSRRAKGRNRLSA
                     "
     gene            13308..14642
                     /gene="dnaA"
                     /locus_tag="PLC71_00730"
     CDS             13308..14642
                     /gene="dnaA"
                     /locus_tag="PLC71_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRM01203.1"
                     /translation="MKNEIEKIWDDVLVKLEQEHDVSSAAINAWIKPLHIKDVQSNKI
                     ILSLDAKYDERGIQFIKKKMYDFYISLAISDLTGKKYDIEFELEKEGEKPQETPAAAP
                     EHDNNNLNPRYTFDTFVIGDNNRMAHAASIAVAEAPAEAYNPLFLYGGAGLGKTHLMH
                     SIAHYIIEHNSSLNVLYVTSEDFTNEVINSIQHKKQEELRSKYRNIDVLLVDDIQFVI
                     GKESTQQEFFHTFNALYNSKKQIILSSDKPPKELDVLEERLRSRFSWGLPVDIQPPNY
                     ETKVAILRKRAELDNIYIDDEIFDYIATHIKSNIRDLEGALNKIKVYSKLNKKPIDLE
                     LSKIALQDLIDNKTKQAITPELIMQTVAEHFNIQTSDIISKKRSHDIAYPRQICMYLC
                     KKMTDQSLVKIGEIIGKRDHSTVIHGIEKIEKDLDKDPELSKVIDVITKKMD"
     gene            14925..16028
                     /gene="dnaN"
                     /locus_tag="PLC71_00735"
     CDS             14925..16028
                     /gene="dnaN"
                     /locus_tag="PLC71_00735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRM01204.1"
                     /translation="MKIICTKSDLVHAVSIVSKAVSNNTTLPILKCILIEASTGNIKL
                     TANDMELGIETTIDGQIHQPGKIAIEAKLFSEIVRKLPDNDITIETDDQYKATITCEK
                     ACFQIMGQEGEEFPSLPEIEKNQSITLSQFSLKEVIRQTIFSISDNENTKLMTGELFE
                     VKDDQLSVVSLDGHRISIRHIELKDHYDPFKVVVPGKTLQEIVKIISGEMDENVSIYV
                     TSKHILFEFDNTRVVSRLLEGEYYKISQMLSSDYETKITINKKEFLNCIDRASLLIRE
                     SDKKPIIIDIADHSLKLNINSFFGSMEEEILIQKEGRDLLIGFNPKFLMDVLRVIDDE
                     EINIYLVNPKAPCYIKDDAGTYNYLILPVNFNH"
     gene            16044..16250
                     /locus_tag="PLC71_00740"
     CDS             16044..16250
                     /locus_tag="PLC71_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRM01205.1"
                     /translation="MEIKLKDEFIKLGQALKAAGLVDSGVVAKIVIQDGEVLVNGEVE
                     TRRGKKLYGGEVVSFDGQEIHIVK"
     gene            16262..17347
                     /gene="recF"
                     /locus_tag="PLC71_00745"
     CDS             16262..17347
                     /gene="recF"
                     /locus_tag="PLC71_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HRM01206.1"
                     /translation="MIIKSLELKNYRNYDELSMNFASGTNLLYGDNAQGKTNILESIY
                     LSATTKSHRGNKDRELIKFEENEAHIRIHFEKQGIDHQLDMHLKKNKAKGVAIDRIPI
                     RRSSDLLGQIPVILFSPEDLKIVKSGPSERRKFLDIELSQMERLYLYQLTNYNKILVQ
                     RNNLLKQIRFQNNLIETLEAWDIQLVKYGSEVIKYREKFIKHLGEVCQKIHNKLTGGK
                     EKILLEYDRDVGYDSYLTELAKKRQKDLKYSTTTVGPHRDDISFIVNGIDIRKYGSQG
                     QQRTAALSLKLAQIQLMREVMKESPILLLDDVLSELDSNRKTYLLESIKDTQTIITCT
                     GLDEFISKHLPIQRMFQIKAGKIVKEN"
     gene            17357..19279
                     /gene="gyrB"
                     /locus_tag="PLC71_00750"
     CDS             17357..19279
                     /gene="gyrB"
                     /locus_tag="PLC71_00750"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRM01207.1"
                     /translation="MGTQKNGEYGADQIQILEGLEAVRKRPGMYIGSTSEKGLHHLVY
                     EIVDNAVDESLAGFCDTIIVTLNKDGSVTVEDNGRGIPVGINKKKGVSSVEVVFTILH
                     AGGKFGGGGYKVSGGLHGVGSSVVNALSDWLEVTVCKEGHIHKQRYERGKVCYPLKVL
                     GDTDKTGTTVTFYPDATIFETIDFDYNTLKRRLRETAFLTKNLKIVLTDDRGEEQVSE
                     TFHYEGGIKEFVTYLNKGKEALYPDVIYCEGEKDGVYVEVAFQHNDSYNETSLSFVNN
                     IITPEGGTHLSGFKMALTSTFNDYARKNNLLKEKEDNLSGEDIREGLTSVISIKIGEP
                     QFEGQTKQKLGNSEARGAVNSILSEQLKYFLEQNPQVAKIICEKAVLAQRARDAARKA
                     RDLTRRKTALDGMSLPGKLADCSDKNPENCEIYIVEGDSAGGSAKTARSRATQAILPL
                     RGKILNVEKARLDKVLVNKEIQAMITAFGTGIHEDFDIEKLRYHKIIIMTDADVDGAH
                     IATLLLTFIYRFMPELIKKGYVYLAQPPLYRVEKNKKFWYAYSDEELNNILQEIGRDN
                     NNKIQRYKGLGEMDAEQLWDTTMDPEKRILLRVNLDEDSASEIDLTFTTLMGDQVEPR
                     REFIEANAKYVQNLDI"
     gene            19294..21777
                     /gene="gyrA"
                     /locus_tag="PLC71_00755"
     CDS             19294..21777
                     /gene="gyrA"
                     /locus_tag="PLC71_00755"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRM01208.1"
                     /translation="MDENTIFDKVHDIDLKKTMENSYIDYAMSVIASRALPDVRDGLK
                     PVQRRILYAMIELNNGPDKPHRKCARIVGDTMGKYHPHGDSSIYGALVNMAQEWSTRY
                     PLVDGHGNFGSVDGDGAAAMRYTEARLSKISMELLADINKNTVDFRPNFDETEKEPAV
                     LPSRFPNLLVNGTQGIAVGMATNIPPHNLREVINAVIKIIDNQVEEDRETTIEELLEI
                     IKGPDFPTGATILGRSGIDQAYRTGRGKIKVRAVTDIEAMANGKQRIIVTELPYMVNK
                     ARLIEKIATLVREKKVEGITELRDESDRSGMRICIELRRDANANVILNQLYKHTQLQD
                     TFGVIMLALVDGQPKTMNLHEMLDYYLTHQKDVVTRRTRYELNKAEERAHILEGLLIA
                     QDNIDEVIKIIRGAENIQAAKLELMERFGLSDAQAQAIVDMRLRALNGLERAKLEKEY
                     KELMERIGELKAILADEKKLLGVIKDEIALIRDKYGDERRTQIGFDVDDISMEDLIPR
                     ENTVITMTKLGYIKRMTVDNFKSQNRGGKGIKGMQTIDEDYIEELFMTTTHHYIMFFT
                     NTGRVYRLKAYEIPESGRTARGSAIVNLLQLQPGEKITAVIPLVDYEEGKYLLMATKY
                     GVVKKSSIMEYANIRKTGLAAITLKDDDQLIEVKYTEDDEDVFLVTKNGQCIRFHESD
                     VRCIGRTSMGVRGINLTGDDEVIGMQLDSQGEALLIVSANGMGKRTLTSEFTTQHRGG
                     KGIKCYKITEKTGDVVGVKAVNDDNEIMMITTEGIIIRTAVDSISILGRNTSGVKVMN
                     VGENVEVASIAKVREEKLEDEMEKNTTEE"
     gene            21929..22771
                     /locus_tag="PLC71_00760"
     CDS             21929..22771
                     /locus_tag="PLC71_00760"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRM01209.1"
                     /translation="MRTIHTDEIIKNIKEMCIEANLSLTEDMKCRFKNATESEKSPLG
                     KQILNQLQENMEIAAADQIPICQDTGMAIVFLNIGQDVHIEGMDLHDAVNEGVRQGYT
                     EGYLRKSVVKDPLIRENTKDNTPAIMHIDIVPGENLEILVAPKGFGSENMSRIFMLKP
                     ADGEEGIKKSVIQAVKDAGPNACPPMVVGVGLGGSFEKAAFLAKKALTRNLDTPSEKP
                     HIAKLEKELLEEINQLGIGPGGLGGSTTALGLNIETYPTHIAGMPLAVNICCHVNRHA
                     HRVL"
     gene            22847..23401
                     /locus_tag="PLC71_00765"
     CDS             22847..23401
                     /locus_tag="PLC71_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HRM01210.1"
                     /translation="MEKHLNVPANTEELAELKSGDYVYLTGTIYTARDAAHKRMYETL
                     LDGKELPVDVKGQFIYYLGPTPAREGQVIGSAGPTTSSRMDKYTPTMLDQGLKGMIGK
                     GKRSSEVIESIKQNGAVYFAAVGGAGALLSKCIKEAEVIAYDDLGTEAIRKLRVENFP
                     VIVVIDNQGNNLYETAVENFKKLK"
     gene            23501..23588
                     /locus_tag="PLC71_00770"
     tRNA            23501..23588
                     /locus_tag="PLC71_00770"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23535..23537,aa:Ser,seq:gct)
BASE COUNT         7362 a         4489 c         4081 g         7947 t
ORIGIN      
        1 ttatttttta ggctgcagag attcttttcc acccatatat ggtcttaaaa cttctggaat
       61 tgcaacgctt ccatcttctc ttaagttgtt ctctaagaat gcgatcagca ttctaggtgg
      121 tgctactact gtattattta atgtatgcgc aaagtaattt ccattttcac ctttgatacg
      181 gatgcgcagt cttcttgcct gtgcatcacc aagattagaa cagcttccta cttcaaagta
      241 tttcttctgt cttggagacc aagcttctac gtcaacagat tttactttca gatctgctaa
      301 atcaccagag cagcactcta atgtacgaac tgggatatcc atagaacgga ataaatcaac
      361 tgtgttcttc cataattttt cataccaatc cttgctttct tctggtttac atacaacgat
      421 catttcctgt ttctcaaact gatgaattct gtaaacacca cgttcttcaa taccatgtgc
      481 tcctttttct tttctgaaac atggagaata gcttgttagt gtttttggta attctgcttc
      541 ttctgttgtt gtatcaataa atttaccgat catagaatgt tcacttgtac cgatcagata
      601 taagtcttcg ccttcgatct tatacatcat cgcatccatt tcatcgaaac tcataacacc
      661 tgttacaaca ttgcttctga tcatgaaagg tggtacacag taagtaaatc cacgatcgat
      721 catgaagtct cttgcataag caatgactgc agaatgtaat cttgcgatat ctcccatcag
      781 ataatagaat ccattacctg caacttttct tgcactgtca agatcgattc catcaaaact
      841 ttccatgatt tctgtatgat atggaacttc aaaatcagga acaactggct caccaaatct
      901 ttcaagttct acgttttcac tgtcatcttt tccgattggt acagatggat caatgatatt
      961 tggaattgtc atcatgatct ctttgatctt ttcttcaaca actttttctc tttcacttaa
     1021 ttcttcaata cgatcagcag atgctgtaac ctgttttttt acttcttctg cttcttcttt
     1081 tttaccctgt cccatcagtg caccgatctg tttggataat ttattacgat ccgcacgaag
     1141 tgcctgaact tcctgtttga tctcacggtt ttctttatcc agttcaatga cctcatcaac
     1201 taacggtaat ttacgatcct ggaatttgtt tttgatgttc tgttttacta catctggatt
     1261 ttctcttaaa aatttaatat ctaacatttt ttcctccaat tatttattga tacatttttc
     1321 taatgtcaat ttttcctcat ttgataatgc tacttttgaa gcaccatttc taatttcttt
     1381 tgcatagaca taagttgcat tctgactgct cattccattt aaaagtgttc cattattatt
     1441 atggtcaagt aatgcgatta caaagcttgt ttctccaccc atatcagaaa atgcattata
     1501 tttatagact tccatcttag aaaaactact cttatgctct tcgtcaattc tctgtatttc
     1561 ctgttcaaga aagtcttgtt ctacaatgat ctcatttaat ttatcaaatc ttttaacgat
     1621 actttcctct aacgattttc catctttccc agataaaaac ttttcatatc gtttttttaa
     1681 tttcccaact tttactaaag caacgatcac aagaatcagt gctaaaagta atgctgctgc
     1741 aagaccaatc actacatatt ccatgcctac actgttaaaa aaatctgtca tcttatgttc
     1801 ctcctaaatt gatctgatca tatccacgat acgttctaac tcatcgcgtg aataatactc
     1861 aatttctatt ttccctttat tattcttctt atttttgata ctgactttac ttcctaaaat
     1921 ctgtttcata gattcttcta attcagtgta aaggaaatca tgaacatctt tttcttcttt
     1981 tttatttttt ttgattttct tcagatcttt taccagcttt tcaatgtctc taacacttaa
     2041 tttttcatca aaaactttct gagcaatttc atattgttta tccttatttg agattgctaa
     2101 taaagctctt gcatgtcctg tgctgatcat ctcttcctct agcatcttct gtactcttgg
     2161 atctagtttt aaaagtctta atgagttggt aattgatgca cgactcttac ttactttcgt
     2221 cgcaacttca tcctgtttta attcaaactc ttccatcaat cgcttataag ccattgcttc
     2281 ttcgattgga tttaagttat ctctctgaat gttttcaatc agtgcaattt ctacaatttc
     2341 attttcattg tattttctga tcataactgg aacttcattc aatcctgcct gttttgcagc
     2401 tctccatctt ctctcccctg caatgatctc atagaaatca tcctttggag taacgaggat
     2461 tggctgtaac attccatact gtttaataga atctgcaagt tcctgcaacg tatcctcatc
     2521 aaactgattt cttggttgat ctggattcgg ctcaatctta tcgattggca aagtaagttc
     2581 ctttttcgtt tcttcttttt tctttgtttt cgacgatctc gaaacttttt tctttacctg
     2641 ctcaacgtct tcttcgtcaa cgattggtgc ttttggaatc agcgtattta aacctcttcc
     2701 aagtcccatt ttttttgctg acattaattt tcacctccat tttcgataac ttcttctgcc
     2761 agaaggtcgt aactttctgc tcctgaagaa cgagggtcat acaaattgat tggaagtcca
     2821 tgacttggtg cttctgccag acgtacattt cttgggatga tcgttttgta aatattctga
     2881 ttcaaatatg attttacatt ctcaacaacc tgcaatgaca gatttgttct ggcatcatac
     2941 attgtaaata caacaccctc tattgtaaga tctggattca aactcttctg aatcaattca
     3001 atcgtataga tcaactgagt taatccatct aatgcataaa attcacattg aattggaacg
     3061 atcactgtat tcgctgctgt catcgcatta accgttagca ttcccaatgc tggcggacag
     3121 tcaataatta tgaaatcata ttgtttcttt actttctcaa gattatgttt catgatgaac
     3181 tgcatcttat caacggtcat caattcgatc tcggctccgg ccagatttcg atttgctgga
     3241 agtaaggata aattatcaaa caagtcttta acgatacaat tctgtatctt ctcttctcct
     3301 gtgatcacct cataagttgt cttatccagc tcgttttttt ctaatcccaa gccactggtg
     3361 gtattcccct gtgggtccat atcaacgagt aaagtctttt ttcctcttgc agctaatgct
     3421 gccgaaagat ttaccgctgt cgttgtcttc ccaactccac ctttctggtt ggcaattgca
     3481 ataattcttc ccattttttt ctccttttga acaatattat aacactaatt atttttcatt
     3541 gctacatgtt tcacgtgaaa catttattaa tttttgtgca atttcgcttt tataactatt
     3601 aagattcttt tcgtttttat attgaatgtt tcacgtgaaa catttattaa ataatcaatg
     3661 catactacta tttttcaaga agaaaaatgg agtgtcttca caaataagaa cactccataa
     3721 ttccacatat tttttaaaat cttatcatct gctatttatt tactcaaatt tgattttatt
     3781 acatttgttc agggcactga attcctaata gatccaaacc atctttaatt acttgttttg
     3841 ctaagatcac aatcttaact ctcgcttctt ttaacttctg atcttccaca ttacactggt
     3901 tttcatgata gaaacggtta aaactttgag ctactcccat tacatatctt gagatcacag
     3961 atggttctaa tctatctgct gccaccttga tcacttcagg gaatctttca atctctttta
     4021 ataatgcaac agatgtttca tctgttacta aagatggatc gatctctcca acttctgtga
     4081 ttcctgtttt tcttaataca cttgccgcac gtgcatatgt gtactgaaca taaggacctg
     4141 tttccccatc aaagctgtgt atcttctccc atgtaaatgt tacatcttta attctctgat
     4201 tatataggtc attaaagata attgctccga ttccaacttt ctgtgcaaca tcctctttat
     4261 ccttcagatc tggattcttc tctgcaataa tttctctgat cttagatact gattctttta
     4321 agatatcttc tgcatatacg atgttaccac ttcttgtaga aagttttcca ccttctaaac
     4381 ttactaatcc ataaggtaca tgaattaagt tttctttagc ccattcatat cccattaact
     4441 caacaacttt aaatacttga gcaaaatgta atttctgctc taatcctgta acataaatac
     4501 atttatcaaa gttatattct ttcttacgat agaaaatagc agcaagatca cgagttgcat
     4561 aaatactgct tccgtctttc ttcataacca gacaaggagc catatcatat tcatcaagat
     4621 caacgatcat tgctccttca ctttctttta gcagattctt ttcttttaat tcttctacaa
     4681 ctgctgctgt cttatcacga tagaaacttt ctcctgtata atgatcaaaa tccatattca
     4741 gcatcttata aattctctta tattctacca tactgatatc tttgaaccat tcccagatag
     4801 ctaaagcttc ttcatctcct tgttccatct tcgcaaacca ttcacgtgcc tgatcattta
     4861 atgtatcatc tttctctgct tcttcatgaa attttacata gattcttaac agttctggaa
     4921 tacctttttc ttcaactgct tctttgcttc cccatttttt atatgcaacg atcagtttac
     4981 caaactgtgt tccccaatca cctaagtgat taattctttc taccttatat ccaagcttat
     5041 caaaaatatg atataaagag tttccaataa tcgttgttct taaatgtcca acgtggaagt
     5101 tctttgcaat gtttggagaa gaataatcga tacatactgt ttttcctttt ccaatttcct
     5161 gtgcaccata agtaccgttt acctttgcca ttacttcttt tacataattc tctttattca
     5221 agaaaaagtt tacatatggt cccattgctt ctacttttga tacatcttca cttgcaggaa
     5281 tcttttctgc aatctctgtt gcgatcatat tcggagcttt acgaaatact tttgctaact
     5341 gaaaacatgg aaatgcaaaa tctcccatat catcttttgg tggtatttca agaatcccat
     5401 caattgtttc tttatctaat tcttcaattg tttgatctag tagatcaata actttatttc
     5461 tcataatctt ccctttctac atctctatca tagtctggat caccactgca tttgttttcc
     5521 atcgttcaat agaaaattct tttgttagtt tattttctaa aacagaacgc ttctcataga
     5581 aatcttttgc attttttatc acttttggtt ttgcttcaat attaattaat attttatact
     5641 catcaatatt catttcaaaa ttaagaacaa aaacacctat ctcatttaaa agactctcca
     5701 acactgcctg aaaacctttt aaaaatgaaa atgtcatgca tacttttgaa ctctctgatg
     5761 aatcctgata attgatacgc tgtgttggat aaagtttctt aacatatccc atcaactgtt
     5821 caaatgctgg aacaaacaat attttctgat cttccctttc cacaaattct tctgcaaaaa
     5881 ggcaagtctc ttccagtccc ttctggtaat catgtaattt tgttatcttt tcttcttttt
     5941 cacgcaggat catctgaaat tcatgaatct ctttctcaat ctgaatcata tcctcagaaa
     6001 caggctgctt ctcctcctca atctccagtg gaagcggaga aaataccttt cggatctgat
     6061 aaatcttctg ctgattattg atcgcacctt ctttttcatt cttctcctga atcatatcct
     6121 gcatcttatg attcagttga tttcgatcag ttttcaattc ctgaatctgt tcttccaaat
     6181 agtcatataa ctgatcgatc aggtgacttt tatctttgtt tttttccata attttcccac
     6241 tttcttttta tcataaacga taaggaaaag aaatgaaagt ataaacttct agcaaatcgg
     6301 ttcttttgtc ggcaatcctg cttttctcgg aaaacgtttt ggtgttgctt ttgtcttatg
     6361 aatctttaag atcgttctcg ccatatccgt atccggaatc tcaaaagaaa taatatcctc
     6421 aatgctgccg cctaaaattt tcactgcttt ctttgaattt gctgcttcct ctttaatatc
     6481 tcctgattta tatggcagaa aatatccatc ctcttttaca taaggaagac aatattctga
     6541 taaaactgtc agattggcaa cagcccttga aacaacataa tcaaattgtt ctctatattc
     6601 cttttgtttt gcaaaatctt ccgctcttcc atgaattgct gtaatctttt caagtccaag
     6661 tgcttctatc acttcattta agaatttaat acgcttattt aaagaatcca gtaacacaat
     6721 ctctaattct ggaaatgcga tctttaatgg aattcctgga aatcccgcac ctgttccaat
     6781 atcaagaatt ttctgaccag ttaaagaaac tccactcttg atcaatgcaa gactatcagc
     6841 aaagtgttta tcgatcactt cattcttctc tgtgattgct gtcagattca tcactttatt
     6901 cttctcaaca agcatctcat agtatgtttc aaactgctct aactgatgat cgctcaattt
     6961 aattccaaat gcagctgctt tttctcttaa tctctccatc tattatctcc tgtggtgcat
     7021 ctgttccaga taaatcagta atacagaaat atccgctggt gatactcctg aaattctaga
     7081 tgcctgtccg atggaagatg ggcggatctt tgataacttt tgtttcgctt caattcgtaa
     7141 gcttccaaca tcttcgtaat caatatcttc cggaattctc ttcttttcca tcttcttaaa
     7201 ctgttttacc tgtgacagct ggcgtttgat atatccttca tatttaatat taatatttac
     7261 ctgttcctga acatccagat catactctgg tctctctgga tcgatcggtg ccaaatccat
     7321 ataagtaagc tccggtcgac ggatcagttc tgtcatttta gcagatgatt ttaatgttgt
     7381 acttccaagc tcttcaagta atgactgcac atttttagac gcacccacat taatatcctc
     7441 tagtcgtttc atctcatctt cgatctgctg ttcttttact aataatttat catatctttc
     7501 ctgactgatc agtcctgctt catatccgat cttggtcaga cgaagatcgg cattatcctg
     7561 acgaagcaac aatcgatatt ctgctcttga tgtcatcata cgatatggtt cctgtgtttc
     7621 ttttgttacc agatcgtcga tcaaaactcc aatataagcc tgtgaacgat ctaaaataat
     7681 tggatctttt tcttgcagtt tgcgtgcagc attgatacct gccatcagac cttgtgctgc
     7741 tgcctcttca tatcctgaac ttccattaaa ctgcccacca gaaaataacc cgtcaatctc
     7801 tttaaattcc aatgaagctt ttagctgtgt cgcatcaata caatcatatt caattgcata
     7861 agcatttctc acaatcttag cattctctaa tcctggcaca ctgtgataca tcttatactg
     7921 tacatcttcc ggcaacgagc ttgacatacc accaacatac atctcattcg tgtactcgcc
     7981 ctctggctca ataaatacct gatgtctatt cttatctggg aatttcacga tcttatcttc
     8041 gatcgatggg cagtatctag gtcctgttcc ttcaattgca ccagaaaata acggtgatct
     8101 gtgaatattt tctttaatga tctcatgtgt ttcttccgtc gtataagtta accaacagga
     8161 aatctgatct ctcttaatat cttcttcctt atttgtaaaa ctgaatggaa caattctctc
     8221 gtctccaaac tgttctgcca tcttagaaaa atcaatcgta ttcttatcaa ttctggctgg
     8281 tgtccctgtc ttaaatcgtc tcatggagat tccatgactc ttcagggaat ctgtcaggta
     8341 atttgctgcc tgaagtccat ttggtcctgt atgattgctg acttctccat aaatacagcg
     8401 cgcctttaaa tatgttcctg tcgttaagac aacagctttt gcatagtaaa cagctcctga
     8461 atatgtctta acacctttga tcttcccatc ttcaacaaga atctctgtaa cttcaccctg
     8521 acgaagtgta agatttggtg tatttcccat cacctgagtc atggaaatag aatacatctt
     8581 cttatctgcc tgtgctctta aagaatgcac tgccggccct ttcgatttat tcaacatctt
     8641 agactgtaga taagtcttat caatattttt acccatctca ccgcccaatg catcaatttc
     8701 ttttaccaga tgtcctttgg aacttcctcc aatattagga ttacatggca tcaatgcaac
     8761 actatccatg cttactgtaa aacaaatcgt ttcaaatccc attctggctg ctgcaagtgc
     8821 agcttcgcat ccggcatgac ctgcacctac aacaataata tcatattttt cttctaaatt
     8881 attcatcttt atttacccat acaaaattct gcaaaaatct catttaccag atcttctccc
     8941 acagattctc ctgtgataaa tcctaaatgc tcatatgcat ccaaaagatc aatggagaag
     9001 aagtcttccg gcattccatc ttcaatactc tgcaacacca tagcaagact ctttaaagct
     9061 tcacttactt ctgttttatg tctcatgttc gtcaaataaa tctcatcatt aaaggataca
     9121 tgtccttcaa agaaaatatc ctggatcagc tgatacaatt cttcaattcc agtctgctct
     9181 ttggctgaaa tctgcacaat ctgctcaaat cctttttctt tgagatctga taccttcacc
     9241 acctgatcaa gatctgcttt gtttaataat acgatcgtct gcttatcttc aatcagctcc
     9301 ataatctctt catcatcttt tgttaatgga tctgaagtat ccacaacata taatacaaga
     9361 tccgcttttg tcaacaaatc ttttgcttta tccacaccga tcttctcaat gagatcattc
     9421 gtatctctga ttcctgctgt gtcaatgata ttcaacggaa tccctttaat ctgaatagat
     9481 tcttctaacg tatctcttgt tgttcctgca atatctgtga cgattgctcg ttcttctcca
     9541 agtaatacat ttaacacaga tgattttcca gcattcggtt ttccaacgat aactgtctga
     9601 attccctctt tcagaattct tccattatca gcattctcca aaaactcatt taactgattt
     9661 atgatcactt cgacttgatc tttcaattca tctgaaaatc cgtccacact gtaatgttcc
     9721 ggatcatcta atgcagattc aataaacgcc acactgtgaa tgatctgttt tcgaatctct
     9781 gtgatcttct ttcctaacgc accctctaac tgctttaaag atgtactcat tgcaaattca
     9841 ttctttgact gaattagatc cataactgct tcagcctcag aaagatcgat ccttccattc
     9901 aaaaatgctc tctttgtaaa ctctcccggc tcagctggcg ttgctcctgc cttaaatact
     9961 gcttccagaa ccttcttcat aacaaccaca ccaccgtggc agttaatctc aacaacatct
    10021 tctgctgtat aagaatgagg tcctttcata actaatacaa tacattcatc tagcaatgtg
    10081 ctttcatcat agacatgtcc ataatgtgcc gtatatgtct ttacatcttt gatcttctta
    10141 tcttttttct tcggtttaaa tactttttca ataatatcaa gtgcctcatc tccactgatc
    10201 ctgataattc caatcccact gttagtcatt ggagtggcaa ttgctgcaat cgtatttgat
    10261 ttcatataat atacctcaaa aaagggctgc ctaaattaag gcagccctat atcttatttt
    10321 ttaagcataa caacaacttt acgataagga tccttacctt cgctctttgt gatcacttct
    10381 ggatctctct gcaatgcaga atggatgatt ctacgctcat ttggattcat tggttctaag
    10441 taaataggtt tcttattctt ctttacttta taagcaatgc ttccagctaa tttctctaaa
    10501 gtctgctgtc ttctttctct ataattttct gtatcaagct taatctttac aaaagaatcg
    10561 ctttctttat tcacataaag acttataaga tactgcaggg catccagtgt ctgtccatgc
    10621 ttaccgatca atgcacccat cttctctcca gatacattga tgtttaatac gttatctctg
    10681 cggttgtagt aaagattcaa ctttggatca agtcccattg ctcttaatac ttcatctaaa
    10741 tatttctctg tatctttgac gatctgatca atgttttctg gtgtcttatt ttttcttggt
    10801 tcctttacag gaacttcatc tttcaccttt ggactcttct taggctgacg atttgatttc
    10861 tcatcgacct tctttgatgg ctttttctca gcttttttct ttggttgttc cttttctatt
    10921 gtctgagact tcataacttc tgtctctttt tcttcttctt taatttcaat gatcacaggt
    10981 ttcttaaata accctaaaaa tccattacta cctgaatcga tcactgtata cttgaggtct
    11041 gtacttggaa ttcctaactc cattgcagca ttcataactg catcagattc agttttacca
    11101 gtaaatctct ttgctgacat ccttatttac ctcccttgtt tttattgagt gcatttgctt
    11161 ttgaagcaat tcctccttta gaatttcctg aatattcaat attctgtgtt gatttcaggt
    11221 ttgcactctt cttgatatta gaatctgctc cttgctgtcc ctgctgttct gcttgcattc
    11281 ctaacatctt ttcatataga gaaggtcctt ttttcttttt cttctttgca gccttctcac
    11341 ggctcttttc aataatctta tccatatcag catgatcata atgataatta aaagcgacct
    11401 gctggatata ctggaataat gcacttgctg accagtaaag accgataccg atcggtaatg
    11461 aaatacacat tacaaatgac attaaaggca ttgtgtacat catacttttt gtcattcctg
    11521 ctcctggtgt gctgtcatcc atttgtgttg tagacataga tgtctttgca cttaagaact
    11581 ggaagataaa agataatact ggaatgatca ataatggtgt taatctgaat cctggtgctg
    11641 aatttaaatc catacctaag aataagtttg gtttaaattg tacatctctt gcgatctgag
    11701 gaatataagt attaactcca cgaattacat aatacagtgc catgatgatc ggaaactgaa
    11761 ttaatgttgg taaacatcca cctgttggac ttgaaccata ttttgcataa accttctgca
    11821 tctcttcctg ctgtttcatc atagatgcct ggtctctttt attacgatat ttttttgtaa
    11881 tcttattaat ctctggctgc atatgctgat tgatcttcat agatctctgc tgtttcatag
    11941 taacaggtaa taagatcaat ttagaaacca atgtaaataa aataatacat aaaccgacat
    12001 tatagattcc cattgcagct aatccatcat agattgctcg aatgatccaa ccaaaaataa
    12061 ttacaaacgg agctaagatt cctccgttac ccgtgctctg ctgggcttgt gccaataaca
    12121 tcattttttg cacctttttc ctttccttac ttccggaaca gggtcatatc cgccttttga
    12181 aaatggatta catcttaata ttctaaaaat tgccaaaaga cttcctttga caaccccgtg
    12241 cttctccaat gcttctatgg catattccga acaagtcgga acatatatac atgttcctct
    12301 tccctttaat ctcgataaat attttctgta aaacctgata atggatatta aaatcttctt
    12361 caattgtaat caactcttct tcagatgatg caaatctatc aaatgtagaa atgcactttc
    12421 aattgtatgg taattctgtc cttttgcagt ttgtcttgca attactacca tatcatatcc
    12481 tgttttcaaa ttatcctgat tcaaacgata gctttcacga attaagcgtg tagttctgtg
    12541 tctgactaca ctatttccta cttttttact tactgatatt cccagacggt tatattctag
    12601 atcattcttc ctataataaa ggaccaagta cctgttggct ttggagtttc cttcattata
    12661 taccatctgg aattctctgt tatttcttaa tgaatgataa ttcttcatgg ttctcttcct
    12721 tattaataga gaaaagaaaa ggtcacatct ctgcgaccta agctgataat ctatttcttc
    12781 cttttgctct tctgctcttc agaacttttc ttccgtttgc agtgctcatt ctttttctaa
    12841 atccatgaac cttagatctc tgtcttttct ttggctgaaa tgtcattttc atgctaaaac
    12901 acctccttaa caaaatttta tgattatata tctgaataat atattcgtaa aaacatcact
    12961 cctattatag acagagaaac gccaaaaagt caagtaaatt ctaagctttt ttcactttct
    13021 tatagaattt catatatatt gtgttttacg cacaagttat ccacaatttg tagataactt
    13081 gtgtataact tggtgataaa atgtgaatta taaacacagt atttgtcaaa ttcctttgtt
    13141 ttttctcgtt ttttgacatg tgtataatgt ggaacaactt atccacaata tgtgggcaac
    13201 ttttattttt cgattgaaaa aagaaatttt ttgttataca ctatatgcat agtgccgtat
    13261 tatgcacatt tataaaaata tgatttagaa agggatttat gttatccatg aaaaacgaaa
    13321 ttgaaaaaat ttgggatgat gtattagtca agcttgagca agagcatgac gtctccagtg
    13381 ctgcgatcaa tgcctggatt aagccattac atatcaaaga tgtccagtca aataagatca
    13441 ttctgtctct ggatgcaaaa tacgatgagc ggggtattca gtttatcaag aaaaaaatgt
    13501 acgattttta tatttccctt gctatttctg atctgacagg aaagaaatac gatatcgagt
    13561 tcgaacttga aaaagaagga gaaaaaccac aagaaactcc tgctgctgca cctgaacatg
    13621 ataataataa cttaaatccc cgctatactt ttgatacatt cgtaatcggc gacaataacc
    13681 gcatggcaca tgccgcaagt attgctgttg cggaagctcc agcggaagcc tataatccgc
    13741 tgtttttata tggaggtgct ggtcttggaa aaactcattt aatgcactcc attgcacatt
    13801 atattattga acacaacagt tctttaaatg ttttgtatgt aacaagtgaa gactttacaa
    13861 acgaagtcat caattcaatt caacataaaa aacaagaaga attgcgcagc aaataccgca
    13921 atatcgatgt tttattggtc gatgatattc agtttgttat tggtaaagaa agtactcagc
    13981 aggaattttt ccatactttt aatgcacttt ataactctaa aaaacagatc attctttctt
    14041 ctgataaacc tccaaaagaa ctggatgttt tagaagagag acttcgaagc cgtttcagct
    14101 ggggactacc agttgatatt cagcctccaa actatgaaac aaaggttgcg atccttagaa
    14161 aacgtgcgga attagacaat atttatattg atgatgagat ttttgactat attgctaccc
    14221 atattaagtc taatatccgt gatctggaag gtgccttaaa caagatcaaa gtttattcta
    14281 aattaaataa gaaaccgatc gaccttgaat tatcaaaaat cgcccttcag gatctgatcg
    14341 acaacaagac aaaacaagct attacacctg aattgatcat gcaaacagtt gcagaacatt
    14401 ttaatattca gacaagtgat atcatttcca aaaaaagaag tcatgatatc gcctatccac
    14461 gccagatttg tatgtattta tgcaagaaaa tgacggatca gtctcttgtt aagattggtg
    14521 agatcattgg aaaacgtgat cattctactg tcattcatgg cattgaaaaa attgaaaaag
    14581 acttagacaa agaccctgaa ttaagcaagg ttattgatgt tattaccaaa aaaatggatt
    14641 aatttttatt ttttccacat tttttcgata gttatccaca ttttccgtgg ataacctgtg
    14701 gatatcctgt gtattagtat ttttgctgat tttggtgatg ttgataaatc tatgattatc
    14761 ctctgatttt catcaagttt taaacatttt atcaaccctt ataacccttt atacttagcg
    14821 gttttttaag gttatcccca aatccacagc tactacgact attactacga cttttattta
    14881 tttatttaac aacaatctga caccgaaagg agttatacac atttatgaaa atcatatgta
    14941 caaaatctga tttagtgcat gctgtttcta ttgtttccaa agcagtatca aacaacacta
    15001 cgcttcccat cttaaaatgt attttaattg aagctagtac aggaaatatt aaattaacag
    15061 cgaatgatat ggaacttgga attgaaacta ccattgatgg acagattcat cagcctggta
    15121 aaattgcaat tgaagcaaaa cttttttctg aaatcgttag aaaattaccg gataatgaca
    15181 taacaataga aacagatgat cagtacaaag caacgatcac ttgtgaaaaa gcatgttttc
    15241 agattatggg acaagaaggt gaagaatttc cttctttacc agagattgaa aaaaatcaaa
    15301 gtatcacttt atcccagttt tctttaaaag aagtgattcg tcaaacaatt ttttcgattt
    15361 cggataatga aaatacgaaa ttaatgacgg gtgaattatt tgaagtaaaa gatgaccaat
    15421 taagtgttgt atctttagat ggacatcgaa tttccatcag acatattgaa ttaaaagatc
    15481 attatgatcc gtttaaagtt gtcgttcctg ggaaaacttt acaagaaatt gtgaagatca
    15541 tttctggaga aatggatgaa aatgtttcaa tttatgtaac ttcaaaacat attttatttg
    15601 aatttgataa tacaagagtt gtttcaagat tgctggaagg ggaatattat aagataagcc
    15661 agatgttatc tagtgattat gaaacaaaga ttacaattaa caaaaaagaa tttttaaatt
    15721 gtatcgacag agcgagtctt ttgattcgtg aatctgataa aaaaccaatt attatcgata
    15781 ttgctgacca ttcattaaaa ttaaatatta attctttctt tggatctatg gaagaagaaa
    15841 ttctgatcca aaaagaagga agagatctgt taattggctt taatccaaag tttttgatgg
    15901 atgttcttcg agtgatcgat gatgaagaaa tcaatattta tctggtaaat ccgaaagcac
    15961 cttgttatat caaagatgat gcaggaacat acaattatct gattcttccg gttaatttta
    16021 atcattaaga aaggactttt gagatggaaa ttaaacttaa agatgaattt attaaacttg
    16081 gacaggcatt aaaagcagct ggacttgtag attctggagt tgtggcaaaa attgtcattc
    16141 aggatggtga agttcttgtg aatggagaag ttgaaacaag aagaggaaaa aaattatatg
    16201 gtggcgaagt tgtatctttt gatggtcagg agattcatat cgtcaaatag tttggagtag
    16261 tatgattatt aaatctttgg aattaaaaaa ttatcgaaat tatgacgaac tttccatgaa
    16321 ttttgcgagt ggaacgaatt tattgtatgg agataatgcc cagggaaaga ccaatattct
    16381 ggaatcaatt tatttaagtg cgacaacaaa atcacaccgt ggaaataagg accgggagtt
    16441 gataaagttt gaagagaatg aagctcacat aagaattcat ttcgaaaaac agggaattga
    16501 tcatcagtta gatatgcatc ttaaaaaaaa taaagcaaaa ggtgtggcaa ttgacagaat
    16561 tccgattcga agatccagtg atctgttggg acagattccg gtaattttat tttcgcccga
    16621 agatttaaag attgtaaaga gtggtccaag tgaaagaaga aaatttctcg atattgaatt
    16681 atcccaaatg gaaagattat atctgtatca attaacaaat tataataaaa tactagttca
    16741 gagaaataat ctgttaaaac agatacgttt tcagaataat ctgatagaaa cattagaagc
    16801 atgggatatt cagctcgtaa aatatggctc agaggttatt aaatatcgtg aaaaatttat
    16861 caaacatttg ggtgaagttt gtcaaaagat tcacaataaa ttaactggcg ggaaagaaaa
    16921 aattttactg gaatatgacc gcgatgtagg gtatgatagt tatttaacgg aacttgcaaa
    16981 gaagagacaa aaagatttaa aatattctac aacgacagtg ggaccgcaca gggatgatat
    17041 cagttttatt gtgaatggga ttgatatccg caaatatggt tcccaagggc agcagcggac
    17101 agcagcgctc tctttgaagc tggcacagat ccagttgatg agagaagtga tgaaagagtc
    17161 accgatcctg ctgcttgatg atgttttatc tgaacttgac agtaatcgta agacgtattt
    17221 actagagagt atcaaggata cgcagacgat cattacctgt acaggtctgg atgaatttat
    17281 tagtaaacat ctgccgatcc aacggatgtt tcagataaaa gcaggaaaaa ttgtaaagga
    17341 aaattaggag gaaactatgg gtacacaaaa aaatggagaa tatggagctg atcagatcca
    17401 gattctggaa gggttggaag ctgtacgtaa aagacccgga atgtacattg gtagtacatc
    17461 agaaaaagga cttcatcatc tggtatacga aattgtagat aatgccgtag atgaatctct
    17521 ggctggattc tgtgatacga tcatcgtaac attgaacaaa gacggctctg taacggttga
    17581 ggataatggg cgtggaattc cggttgggat caataagaaa aaaggtgtat ccagcgttga
    17641 agttgtattt acgatccttc atgcaggagg taaatttggc ggtggaggat ataaagtatc
    17701 cggaggtctt catggtgtag gatcttctgt ggttaatgct ttatctgact ggttggaagt
    17761 tacagtctgc aaggaaggac atattcacaa acagcgttat gagagaggga aagtatgtta
    17821 tccgctgaaa gttcttggag atacagataa aactggaaca acggttacat tttatccaga
    17881 tgcaacgatt tttgagacga ttgattttga ttataatacg ctgaagagaa gattaaggga
    17941 gacagctttc ctaacaaaga atttaaaaat cgtcttaaca gatgatcgtg gggaagaaca
    18001 ggtttctgaa acgttccact atgaaggtgg tattaaagaa tttgtgactt acctgaacaa
    18061 ggggaaagaa gcactttatc cagatgtgat ctattgtgag ggtgaaaaag atggtgtgta
    18121 tgttgaagtt gcattccagc ataacgattc ttacaatgag acatccttaa gttttgtaaa
    18181 taatatcatt acgccggaag gtggtacaca tttatctgga tttaagatgg cattgacatc
    18241 aacgtttaat gattatgcga gaaagaataa ccttctgaaa gaaaaagaag ataatttaag
    18301 tggtgaagat attcgtgaag gattgacttc tgttatcagt attaagatcg gggaacctca
    18361 gtttgaaggt cagacaaaac agaaattagg aaatagtgaa gcgaggggtg cagttaacag
    18421 tattttatct gagcagttga aatatttctt agaacaaaat cctcaggttg cgaagataat
    18481 ctgtgagaag gctgtacttg cacagagagc aagagatgct gcgagaaaag cgcgagatct
    18541 tacaagaaga aagacagcac ttgatggtat gagtcttcct ggaaaacttg ccgattgttc
    18601 agataagaat ccggaaaact gtgagatcta tatcgtcgag ggagattctg cgggtggttc
    18661 tgcaaagaca gctcgttccc gtgcaactca ggcaatcctg cctcttcgtg gtaaaatctt
    18721 aaatgttgag aaggcaaggc ttgataaagt acttgtcaat aaagagattc aggcaatgat
    18781 cacagcattt ggtacaggaa ttcatgaaga ttttgatatc gagaaattaa gataccataa
    18841 gatcatcatc atgacagatg ccgatgttga tggtgcacat atcgcaacac tgcttctgac
    18901 gttcatatac cgctttatgc cggaattgat caagaaaggg tatgtatatc ttgcccagcc
    18961 tcctctttac cgtgtagaga agaataagaa gttctggtat gcttacagtg atgaggaatt
    19021 aaacaacatt cttcaggaaa tcggacgaga taacaataac aagatccagc gttacaaagg
    19081 tcttggagag atggatgcag aacagttatg ggatacaacc atggaccctg agaagagaat
    19141 tttattaaga gtaaacctcg atgaagattc agcttcagag atcgatctga catttacaac
    19201 actgatgggt gatcaggtag aaccaagacg agaatttatt gaagcaaacg caaaatatgt
    19261 acagaatctt gatatttagg aagggagaaa caaatggacg agaatacaat ttttgataaa
    19321 gttcatgaca ttgacctcaa aaaaacaatg gagaattcat atattgatta tgccatgagc
    19381 gttatcgctt ccagagctct tccagatgtg cgtgacggtt tgaagccggt acagagaaga
    19441 attctttatg cgatgataga attaaataac ggacctgata aaccacatcg taaatgcgct
    19501 cgtatcgtcg gtgatacaat gggtaaatat catccacatg gtgacagctc tatctatggt
    19561 gcgttagtta atatggcaca agaatggtcc acgagatatc cattggtaga tggacatgga
    19621 aactttggtt ctgttgatgg tgatggtgca gctgccatgc gttatacaga agcaagatta
    19681 agcaagattt ctatggagct tctggctgat attaacaaga atacagttga tttcagacca
    19741 aactttgatg agacagagaa agaaccagct gtcttacctt ctcgttttcc aaacttatta
    19801 gtgaatggta cacagggaat cgcggttggt atggctacga acattccgcc tcataacctt
    19861 agagaggtta ttaatgcagt tattaagatc attgataatc aggttgagga agatcgtgag
    19921 acaacgattg aagaattatt agagatcatt aaaggaccgg attttcctac aggagcgacg
    19981 atccttggac gttctggaat tgatcaggct taccgtacag gccgtggaaa gattaaagta
    20041 cgtgctgtta cagatattga ggcaatggca aatggaaagc agcgtattat cgtaacagag
    20101 cttccataca tggtaaacaa agcaagactg atcgagaaga tcgcaacttt ggtaagagaa
    20161 aagaaagtgg aaggaattac agagcttcgt gatgaatctg acagaagtgg tatgcgtatc
    20221 tgcatcgaac ttcgtcgtga tgcgaatgcg aacgtaatcc tgaaccagtt atataaacat
    20281 acacagcttc aggatacatt tggtgtgatc atgctggcac ttgttgatgg acagccaaag
    20341 acaatgaatc tacatgagat gctagattac tatctgacac accagaaaga tgttgtgaca
    20401 agaagaacac gctatgaatt aaataaagcg gaagaacgcg cgcatatctt agaaggatta
    20461 ctgatcgcac aggataacat cgatgaagtg atcaagatca ttcgtggagc cgagaacatt
    20521 caggcagcca agcttgaatt gatggaacgt tttggacttt cagatgcaca ggcacaggca
    20581 atcgttgata tgcgtctgag agcactgaat ggtttggaac gtgccaaact tgaaaaagaa
    20641 tacaaagaat tgatggagcg tatcggtgaa ttgaaggcaa ttcttgcaga tgaaaagaaa
    20701 ttacttggag ttatcaaaga tgaaattgca ctgatccgtg acaaatatgg cgatgaaaga
    20761 agaacacaga ttggatttga tgttgatgat atttccatgg aagatctgat ccctagagaa
    20821 aatactgtga tcacaatgac aaaacttgga tatatcaaga gaatgactgt tgataatttc
    20881 aaaagtcaga atcgtggtgg taaaggtatc aagggaatgc agacgatcga tgaggattac
    20941 atcgaagaac tgtttatgac aacgacacat cactatatca tgttctttac aaatacagga
    21001 cgtgtatatc gtctgaaagc atatgagatt cctgagagtg gaagaacagc acgaggaagt
    21061 gcaatcgtta atctgcttca gttacagcca ggagagaaga ttacagcagt gattccactt
    21121 gtagattacg aagaaggaaa atatctgtta atggcaacga aatatggtgt tgttaagaaa
    21181 tcatctatca tggaatatgc aaatatccgt aagacaggat tagcagcaat cacattaaaa
    21241 gatgatgacc agctgatcga agttaaatat acggaagatg atgaggatgt attccttgta
    21301 acgaagaatg gacagtgtat ccgtttccat gagagtgacg tcagatgtat tggacgaact
    21361 tctatggggg ttcgtggaat caatctgaca ggtgatgacg aagttattgg aatgcagctt
    21421 gacagtcagg gagaagcgtt actgatcgta tctgcaaatg gtatgggtaa acgtacactg
    21481 acttctgagt ttacaacaca gcatcgtggt ggtaaaggaa ttaagtgcta caagatcaca
    21541 gaaaagacag gtgatgttgt aggtgtgaag gctgtgaatg acgataatga gattatgatg
    21601 atcacaacag agggaattat catacgtaca gcggtagatt ctatttctat tcttggaaga
    21661 aatacttctg gagtaaaagt tatgaatgtt ggtgaaaatg ttgaagtagc cagcatcgca
    21721 aaagtccgtg aagagaagct tgaagacgaa atggaaaaaa atacaacaga agaataaaaa
    21781 taaaaatcag ggcgtagatg aattgtctat gtcctgattt cgttatataa taaaacaatg
    21841 ataccaaggt caaaatctga tgcagcttta tttgcaggaa aatttatgat attttggcag
    21901 tgacaataaa atatttttgg aggaaaacat gagaacgatt catacagatg agatcatcaa
    21961 gaatataaaa gagatgtgca tcgaggcaaa tttatctctg acagaagata tgaaatgccg
    22021 ttttaagaat gcaacagaaa gtgaaaagag cccattagga aaacagattt taaatcaatt
    22081 gcaggaaaat atggaaattg cagcagcaga tcagattcca atctgtcagg atacaggaat
    22141 ggcaatcgta tttttaaata tcggacagga tgttcatatt gagggtatgg atttacatga
    22201 tgcagttaac gaaggggtgc gtcaggggta tacagaagga tatcttagga aatctgttgt
    22261 aaaggatccg ttgattcgtg agaatacaaa agataataca cctgcgatca tgcatataga
    22321 tattgtccca ggggaaaatc ttgaaatcct agtagctcca aaaggatttg gaagtgaaaa
    22381 tatgagtcgt atatttatgt taaaaccagc agatggagaa gaggggataa agaaatcagt
    22441 aattcaggca gtcaaagatg caggacctaa tgcctgccca ccaatggttg taggtgttgg
    22501 actgggtgga agttttgaaa aagcagcatt tctagctaag aaggcattga cgagaaactt
    22561 agatacacca tctgagaagc cacatattgc gaaattagag aaagaattgt tagaagaaat
    22621 caatcagtta ggtattggtc caggagggct tggaggttca acaactgcgt taggacttaa
    22681 tattgagaca taccctacac acattgcggg aatgccatta gcagtaaata tctgctgtca
    22741 cgtaaatcgt catgcacata gagtgttata aagagttaga tattatctat cgttcagacg
    22801 aaatttttag aatatagtat tttttaaata caaggagtag aagattatgg agaaacattt
    22861 aaatgttcca gcaaatacag aagagttggc agagcttaaa tctggagatt atgtatattt
    22921 gacaggaacg atctatacag ctagagatgc ggcacataaa agaatgtatg aaacgctgtt
    22981 agatgggaaa gaattaccag tagatgtaaa agggcagttt atttattatc tcgggccaac
    23041 accagcaaga gaaggacagg tcattggatc agcaggaccg acaacaagca gcagaatgga
    23101 taaatatacc ccaacgatgc ttgatcaagg attaaaaggt atgatcggaa aaggaaaacg
    23161 atcttcagaa gtcattgaat caataaaaca gaatggtgca gtatattttg ccgcagttgg
    23221 aggagcagga gcacttcttt caaagtgtat taaggaggcg gaagtaatcg cttatgatga
    23281 tctgggaaca gaagcaattc gaaaattaag agttgaaaac tttccagtta ttgtagtaat
    23341 tgacaatcag gggaataatc tttatgagac tgcagtagaa aatttcaaaa aattaaaata
    23401 atcaatcaaa acctattgac aaaaggaaat tactttggta agatattcaa gcgtcatgtg
    23461 aatgacggat gcaattaaat atattgatat ttaggcattt gcagaagtac tcaagtggct
    23521 gaagaggtgc ccctgctaag ggtatagacc gtttacgcgg tgcgagggtt caaatccctc
    23581 cttctgcgct tattaaatct tccaaatagg aggatttaat tttttgacaa aaggtattga
    23641 catggatgta aaagtgtgat atcttataga agttgcactg cttatagcag agtaacaaat
    23701 atcatttaga aagatgatga aaagagcatt tatttatttg aattgctgga cggagaacga
    23761 tcacaacaat tgaataaaat aaattaaaaa actcttgaca gaatgaaatg aaaatgatat
    23821 gatataaaag ttgctgcagt tcttatgaag aactggtcaa caaaataaat caaaaaagt
//
LOCUS       DAOMUE010000006        21917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_382_length_21917_cov_3.215488, whole genome shotgun sequence.
ACCESSION   DAOMUE010000006 DAOMUE010000000
VERSION     DAOMUE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 21917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21917
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_382_length_21917_cov_3.215488"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /gene="nrdD"
                     /locus_tag="PLC71_00775"
     CDS             complement(<1..1495)
                     /gene="nrdD"
                     /locus_tag="PLC71_00775"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRM01211.1"
                     /translation="MIKVIKKDGTKEEFNVQKVVVAVNKSAYRALVTFTEEELDFICN
                     FVTEKVEQMRKKEVTISDMHNVVEGALEQTNPLVAKSYRDYRNYKQDFVQMLDEVYKK
                     SQSIMYIGDKENSNTDSALVSTKRSLIFNELNKELYQKFFMTTEEIQACRDGYIYIHD
                     MSARRDTMNCCLFNVQEVLKGGFEMGNLWYNEPKTLDVAFDVIGDIVLSAASQQYGGF
                     TVPNIELILEPYAEKSYEAGIERYEGLGLSRERAEEEAMKDVKREFEQGFQGLEYKFN
                     SVSSSRGDYPFITMTAGTGTGRFAKMATISMLDVRRRGQGKEGHKKPVLFPKLVFLYD
                     KNLHGPGCELEDVFEAGIRCSRQTMYPDWLSLTGEGYIASMYKKYGKIISPMGCRAFL
                     SPWYKEGGMKPADEKDEPIFVGRFNIGAVSLHLPMIYAKSKQEGKDFHEVLDYYLNLI
                     RELHIRTYEYLGEMKASTNPLAYCEGGFLGGHLGIHDKIKPILKSATA"
     gene            complement(1653..2168)
                     /locus_tag="PLC71_00780"
     CDS             complement(1653..2168)
                     /locus_tag="PLC71_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01212.1"
                     /translation="MKKYRGYLLVLLIAFCIQGCSKQQDVEDHRFVLAMGFERLNEKK
                     VLVRYSYADFDKAQSDSGTKIPSRSVTFLATSLKDANKKWKQYKSQQLNFGHLKVVLF
                     ANGKKDEKIIKELVNEPQIAKSVYVLKTDRNLSDIFKKEDELSISFGEYLSKKLEIKD
                     SKNLTLGRIYR"
     gene            complement(2155..3210)
                     /locus_tag="PLC71_00785"
     CDS             complement(2155..3210)
                     /locus_tag="PLC71_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HRM01213.1"
                     /translation="MKNNSQMITARQGNRIMILEIFSAAAMFLPQIALQKSYHSGIVV
                     VVMASVMAGIYLWIVDHAARGVSIENVLKRYRFAAIIYYVRFLLNGAFFYLCILYLTK
                     KYLLPDKSTFFIGLPLLLLAYLMNQGGLTKRGRVMEGIFWFVLLPLIFVLILSMANLS
                     WDELAVRSWCGREMLNGSILVFALMHPIEFVWFYRGDMKDGPIRMRSFAGLMILFLGV
                     FASTVGSLGKKLTMVDPEPVMSMAQGVAMPGGIMARLDLFLIAFWIVGVFCVFSGYLF
                     YGNESIKHAFSKGRIVGLSLSYGGIYVISPWIMTTFATWIRRYFFVFIYGNLVIGLFF
                     PLILFLMWRKEQKDEKI"
     gene            complement(3200..4711)
                     /locus_tag="PLC71_00790"
     CDS             complement(3200..4711)
                     /locus_tag="PLC71_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HRM01214.1"
                     /translation="MDHFHTRTHNLKGNISPDIQENIKYTTQIMQDCNDLVQKQFKIG
                     VDHEISIYIVYMDGLVNTEMLQESVIRPLLQDSFPQERTAISQYVIESADWKWIDTME
                     DAMTAVLSGNTVLFLDGEARAILFSSKLFPTRGVQNADQEVAIVGPKDSFTESLRMNT
                     ALIRRRIRDTRLKVIQKQIGTRSKTDYALMYIEDLVQKDILNKIQKQMDKICVDGIFD
                     SGMLQQYLEKDSKTPFPLYQLTQRPDKVASSIMEGRIAVVLDNSPMVLLLPVTFNVFF
                     QASDDYYNRWEITTFVRILRYIAAIISIGLPGFYVAIAGFHPEVLPTPFLLALISARE
                     GVPFPVIVEVLLMELSFELLREAGIRLPGQLGGTMGVVGGLIVGQAAVDAHLVSTIVV
                     IVVALTAIATFSIPNELFTSAFRLMKFFLIILCAFWGLYGFFLGFLAIFIHLFYLENY
                     GIPYAHPMVEERGRLSTAFQDFMVRYPLKRMKYRPEFTKEGARVRQKEDEDEK"
     gene            5024..5668
                     /locus_tag="PLC71_00795"
     CDS             5024..5668
                     /locus_tag="PLC71_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRM01215.1"
                     /translation="MAEKLKNKVTDGFQKGHPYGELPPCIHVGILNFNQMISPNYYHK
                     FCLMDEKTKEIYSRKFQFHMLELKKLKYAKEKQQRKPLYQWAKLIAAQTWEELEQESK
                     GNKYMERALEEMIKISQDEMERYLYLREEMAESDRVSQIQSAKRIGRKEGKKEGEILK
                     LITQVQKKIARGDSLEKIADDLIEDETVIKPIYQLVQGNPNKTEDDIYQMLNLL"
     gene            complement(6016..7746)
                     /locus_tag="PLC71_00800"
     CDS             complement(6016..7746)
                     /locus_tag="PLC71_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM01216.1"
                     /translation="MRERLKHKYALSEQGVNDMIKAVIIVTIANLAFMVPVGLLYLLA
                     SDILNGGIPSSKLPMYIGGMVISILLIIITTYFQYNATFLATYVESGVRRRTLAEKLR
                     KIPLSFFGKKDLADLTSTIMGDCAWLETASSHFFPQLFGSMCSTTIVTICLFFFNVKM
                     TLAAVWVLPVAFFVVFGARPISHRLNENAMKYKLECQDGIQEGLETVRDLKSYNATNT
                     YMEGLNKKIQAVEKHAVVSEAINAMFVCLSQLILKVGIGTTTLVGGILFAKGEIDALT
                     FFMYLLVVSRMYDPFQVALENLSAIISTDTQCKRMDEILSHEEQDGNKTLSNQGYDIN
                     FDHVGFSYDGNEQILKDVSFTAKQGEVTALIGPSGSGKTTVSRLAARFWDINKGTITV
                     GGMDISKIDPEILLSLYSIVFQDVTLFNNTIMENIRIGRKDATDEEVMAAAKLANCED
                     FIEKMPQKWDTMIGENGSELSGGERQRISIARAFLKDAPIILMDEATASLDVDNESLI
                     QEALSKLIKDKTVLIIAHRMRTVDGADKIVVLKDGVVAENGTPDELKACDGIYRHMVE
                     LQVEAANWKM"
     gene            complement(7749..9536)
                     /locus_tag="PLC71_00805"
     CDS             complement(7749..9536)
                     /locus_tag="PLC71_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM01217.1"
                     /translation="MEEKKEKEESLTKQLYHFAGSYKYLSILSTILAAISAFVALVPF
                     YYIWKVMQEVLRVRFDFSKAAGISTYGWRAVGFAILGMIIYMAGLMCSHIAAFHVQAQ
                     MRTAMMNHIMTLPLGYIESEGTGKIRKIVTDSSAATETYLAHTLPDKAVSKATPIGLI
                     ILMAVFDWRLGIMCLIPAAIGFVFMSSMSGKDLAESMKQYQNSLEVMSAEAVEYIRGV
                     PVVKTFGQTVFSFKRFKEAIDEYEKWTLGFTKKMRKPMVGFTTAVNSIFVFIIIAAYV
                     FGGHEITAAFGLNLLFYIIITPILTTTLMKLAYAGEAQMQVVDGLKRMGDVMNRQSLK
                     ETTNGQIPKDSSVSLRDVTFAYEGAKKNAVDHISIEIKAGEHVAFVGPSGGGKTTLAS
                     MIARFWDVKEGSVKIGGVDVRDITSSELMNQISYVFQDSKLLKMSILENVRLGKPDAT
                     DEEVMQALKDAQCMDIIDKFKDGVHTMIGAKGIHVSGGEAQRLSIARAFLKNAPVLIL
                     DEATAFADPDNEQKVQKAFENLSKDKTVIMIAHRLSTVTNADRIYVLKDGAIAEEGSH
                     GELLKSHGIYAHMWEEYNKSVNWKVGGVL"
     gene            complement(9555..10517)
                     /locus_tag="PLC71_00810"
     CDS             complement(9555..10517)
                     /locus_tag="PLC71_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM01218.1"
                     /translation="MNEKREVILPSDFSGEKGKIVCYRISSYLELLYIDIRSRRIPEV
                     QIQAYEGEAVMTVNFSFLGKCEVLLKNGGGTYVDTGEYSIDYGTASREADEFYYPRAQ
                     YHGMELILYPCPELDKELSLNGRNTKMSVELKERFLGRETPFISKADARIEQAVRVIQ
                     DDIEADADMNLLAVDVSRWLQVLCSKRDAVETNRVYYSGSQIQIARKTMDILTEDLSK
                     RYSAATLAKHFGISETSLKNYFQGVYGRGYSELLNEIRMKKAAELILKNSMKISEISD
                     AVGFATQSRFAKAFKNYYGAAPLEYKRKHNLNKDSRQMELSDNK"
     gene            complement(10762..11619)
                     /locus_tag="PLC71_00815"
     CDS             complement(10762..11619)
                     /locus_tag="PLC71_00815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRM01219.1"
                     /translation="MARRRKRRRINKGRVAALLIIILVIAAGIFFVTTRFGRTSYASV
                     NKSMGEYMVKANDDLLGKGTDKELKKSLYVPRKIDKTKKLIAFTFDDGPLKGNTERVL
                     AALEKNDARATFFMLGQNANYYPETVKKVLESGNEVSSHTWNHTYLPKLSAAQVREQE
                     DKTANAIYKACGSKPVSVRPPYGAINENVKKGIDTPLILWSVDTLDWKTKNTDATVKT
                     ILKHAKDGDIVLMHDIHKPTVAAVEKVLPILKKKGYEVCTVSELLEAKGVKAGKGDKV
                     FSATDIIRK"
     gene            complement(11638..13293)
                     /gene="ade"
                     /locus_tag="PLC71_00820"
     CDS             complement(11638..13293)
                     /gene="ade"
                     /locus_tag="PLC71_00820"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRM01220.1"
                     /translation="MMYDVIFKNGNVVDVKNEQILQADIAVKDGKIAGIGHFSGENVI
                     DCTGKYITPGFIDAHVHIESSMATPMEFSKAVMPHGTTTVIADPHELVNVKGNEAMEY
                     ILDAAGDAPLGIYVMMPSSIPATPFETNGADFTAEDMKQWKDHPLVLGLGEVMCYPAV
                     LSKEEQIMKKLELCKGMVIDGHAPGLSGEDLKAYVEAGVMTDHECTSFEEAKEKCLAG
                     MKILVREGSAARNVENIVPGLVKEPEFIAHFMFCTDDKHLDTIENEGHISYNIKKSIQ
                     LGMNPISAVKMATYNAAKTYGLNDIGVLEEGKDADIVILDSLESVEVHSVYKQGRIVN
                     TEFFTKEAKPVNESMLHTVVLKNISKDKIQVKAEGKIPVIGMIPGQIVTKFLQEEVPS
                     KDGLFTPDSEYSKLCVFERHRGTGNAAAAPIKGYGITNGAIATSVAHDSHNIIAAGDN
                     DEDIIKAVQTIEEIQGGYALVSEGRVLGTLPLTVAGLLSQKPAKDIQKGIDELLQKAW
                     KLGISRDIDPFITLSFMALPVIPSLRLTDLGLVDVDTFQLLKS"
     gene            complement(13290..13910)
                     /locus_tag="PLC71_00825"
     CDS             complement(13290..13910)
                     /locus_tag="PLC71_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01221.1"
                     /translation="MRKELHCPQIIKDGKTIGYGGDQEWFPGDFQRLAGCGSVTGSNI
                     AAIYALSREDFKDLYPIKDSQKAADYEQYLNLMQTMYRYMKPGFMGYPLIGRFAKDFK
                     RYAKDHGITLSSEQLFLPKSREQALDFILPALKEDHPVAFLILRHPAHELREDNWHWV
                     TITGFDEEEEKLIWSNCGECEEIDWNMLFDTDKKYYVGIVKFKEEE"
     gene            14028..14651
                     /locus_tag="PLC71_00830"
     CDS             14028..14651
                     /locus_tag="PLC71_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HRM01222.1"
                     /translation="MKNILISLCLALFIISASVTITLNFRPLYYHDISSLKIEETSGF
                     SKKVIRENYDALIDYNQFFYSGKLKLTLPMSREGKIHFEEVKRIFVGIECLCVITLIL
                     SCFLIKRKIRSRNIDFLRSASIITVLLPVIVGILCAFNWDAAFTLFHEIMFRNDYWIF
                     DEATDPVIMILPDAFFLHCAVMIILLILIGSLLCMGFRSFFRHRFML"
     gene            14712..15767
                     /locus_tag="PLC71_00835"
     CDS             14712..15767
                     /locus_tag="PLC71_00835"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase
                     C/glycerophosphodiester phosphodiesterase family protein"
                     /protein_id="HRM01223.1"
                     /translation="MRLSKIKTKEIRYVVWKYRLKRFLPHILTLCLAVIVAGGAFSAY
                     SVYHKKHQKQLAKQSEVQREKDVNTARKKAQKEKTTLKPWYTYHSIAHAMGGLDGKDY
                     LNSIDGFYPAYQKGYRVFEMDILLTKDNVAIGKHQWGRKLSDPTSKKGDPVSYRKFKN
                     TKIYGKYTPTSFLDVLNLMEKYPDFYLMTDSKSTEPADAKKEFNVLVQTAKKVGKEDL
                     LDRVIVQVYNQRMYWAVKSVHPFKHFVYTTYKQPDAAFYKVVKFCKQNGIEAITSPKN
                     DINDYRMELLAKQGIYSYTHSVNNAYFAKEFMKLGVYGVYSDFLSPAQVNNSYIRANC
                     PKFASRYVKTIMPGINQ"
     gene            complement(15892..16923)
                     /locus_tag="PLC71_00840"
     CDS             complement(15892..16923)
                     /locus_tag="PLC71_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM01224.1"
                     /translation="MQNPFTLTFGKSPLEPVERPVQTKEIIDTFTAEQINQQMFIITG
                     VRGSGKTVMMTEISQKLRENDQWIVIELNPATDLLKGLLSKLNSNKVCAGIIKSAKID
                     LSFFGFGVAIEGMSPITDEETAIIAILEKMKKNGKRLLITIDEVTNNEFMQVFAGSFQ
                     IFVRQDLPVFLLATGLYENIDELQNEKNLTFLYRAPKIQLKPLNNRAIMNKYKTIFKI
                     ESEQAAKMAELTKGYPFAFQVLGYLTWNHEGDYEAVIDEYEQYLSEFVYDKIWSELSQ
                     KDRIVARGIAEVDGGKIKDIRERLGMETNEFNPYRKRLIKKGILSGEMRGYVYFTLPL
                     FDEYVIDNS"
     gene            complement(17026..17508)
                     /locus_tag="PLC71_00845"
     CDS             complement(17026..17508)
                     /locus_tag="PLC71_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM01225.1"
                     /translation="MNIRTATIKDLEAVTSVEAECFPPAEAATREELAERLKYYADHF
                     WLMFDGDKLIAFVDGFVTDEEDLTDEMYAKAQLHNESGAWQMIFGVNTIPAYRKHGYA
                     GELIKCAIEDARKQERKGLVLTCKDHLVHYYAKFGFENEGVSESEHGGVKWNQMRLKF
                     "
     assembly_gap    17703..17712
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17773..18447)
                     /locus_tag="PLC71_00850"
     CDS             complement(17773..18447)
                     /locus_tag="PLC71_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM01226.1"
                     /translation="MSDKLKVNMNEYLPLRDVVFNTLRQAIITGEFAPGERLMEIALA
                     NRLGVSRTPVREAIRKLELEGLVVMIPRKGAEVARITEKDLRDVLEVRCSLEELAAEL
                     AAERMDEEGQEKLDQALVEFESAIESGDNAAIADSDMEFHDIIFDATGNPRLIQIIGN
                     LREQFYRYRLEYVKDTDYHIVLLNEHKELVNAIRAGKKEEARKIMKKHITNQEMTVIR
                     NIKEEY"
     gene            complement(18513..19379)
                     /gene="ispE"
                     /locus_tag="PLC71_00855"
     CDS             complement(18513..19379)
                     /gene="ispE"
                     /locus_tag="PLC71_00855"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRM01227.1"
                     /translation="MDHSIVLKSYGKINLGLDVLRRREDGYHEVRMIMQTVGLYDVLT
                     MKKRKDDKIEMTCNLSFLPTDERNLVYKAVKLIKDKYHIKDGVEINLSKRIPVAAGMA
                     GGSSNCAAALKGMNQLFDLGLSIDELCEIGVTLGADVPYCIWGGTALSEGIGEKLSRV
                     DAMPDCYILIAKPGISVSTAFVYKNLDLPALSKHPDIDGMLECLKEKDLTGICNRLEN
                     VLETVTIKEYPIIEKVKKHLMDQGAKGALMSGSGPTIFAIFEDKKTADDAMESLRSIE
                     DIKQAYVVRPIQ"
     gene            complement(19475..21034)
                     /locus_tag="PLC71_00860"
     CDS             complement(19475..21034)
                     /locus_tag="PLC71_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HRM01228.1"
                     /translation="MDFDKKEFYGIVAKAKKQMQITIDQDCNVADTKPDVEKMIQTRG
                     IVKIQETEMMVDRVRIKGEFVFQGLYGTNDTSAYLESLEYSLPFEEYVHIEGVLPSDY
                     VKVKYTIDDINTILINSRKISIRVLLTFSFQITEEMKEKGIIEIHEENVSVLKKDVQI
                     TDLIVNKKDIARLKEELVLPANKANIYQILWTQVDMENLQAKIGEHMIEIQGAMHIFV
                     LYLGEDAQMPVQYARWEIPVDTQLECYECMPGMIGRIGMTLGGQQLEIRPDEDGEERI
                     ISLDVTIDCDIKIYEDHKISYIDDGYSMEETLIPEYHMFDFETLVGKNQAVMKADKRF
                     RLEQESGKLLQVLSVKGSATVDDVEMTAQGLSVEGVWMADVLYLSTEDMTPVMSSSYM
                     EPFTFFVETKNLTGNEDYQLDVRVDQMTAIPTEGEEIEIRASVLFDFIAFHRVRQRIM
                     TDMKQGPLDYDKIREIPGIVGYIVKEGDTLWSIAKAYFTTVESIREQNEDISDIKPGD
                     KLLIVKEMKIF"
     gene            complement(21101..21349)
                     /locus_tag="PLC71_00865"
     CDS             complement(21101..21349)
                     /locus_tag="PLC71_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HRM01229.1"
                     /translation="MTQLDLNRVRQSIKNQIGSKIKISANRGRHKFVTAKGVITQTYP
                     NIFLVELENADGNDQNKTVSFSYQDVITRDVRMMLLEN"
     gene            complement(21656..21877)
                     /locus_tag="PLC71_00870"
                     /pseudo
     CDS             complement(21656..21877)
                     /locus_tag="PLC71_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
BASE COUNT         6058 a         4871 c         3499 g         7479 t
ORIGIN      
        1 aagctgttgc actctttaag attggtttga tcttatcatg aattcctaaa tgtccaccta
       61 agaatccccc ttcacaatat gccaacggat tcgtagaggc tttcatttct cctaaatatt
      121 cataagtcct gatatgtaat tcacggatca ggttcagata ataatctaat acttcatgga
      181 aatctttacc ttcctgtttt gattttgcat agatcattgg caaatgaagg cttaccgcac
      241 cgatgttaaa tcttccgaca aaaatcggct catctttttc atctgccggc ttcataccgc
      301 cttctttata ccatggagat aagaaagcac ggcatcccat cggactgatg atctttccgt
      361 atttcttgta catgcttgcg atatatccct ctcctgttaa agataaccag tctggataca
      421 tggtctgtct ggaacagcgg attcctgctt caaatacatc ttccagctca catcccggtc
      481 catgaagatt cttgtcatat aagaatacaa gttttgggaa taacacaggc ttcttgtgtc
      541 cttcttttcc ctgtcctctt cttctaacgt ctaacatgga gatcgttgcc atcttggcaa
      601 atcttccagt tcctgttcct gctgtcattg tgataaatgg ataatctcca cggcttgatg
      661 atacactgtt aaatttgtac tctaatccct ggaatccttg ttcaaattca cgtttgacat
      721 ctttcatcgc ttcttcttct gcgcgttctc tggataatcc aagtccttcg tatcgttcga
      781 ttcctgcttc ataggatttc tcggcatatg gctctaagat cagttcgata ttcggtaccg
      841 taaatccacc atactgctgg cttgcggcac ttaatacgat atctccgatc acatcaaagg
      901 caacatctaa tgtctttggt tcgttgtacc agagatttcc catctcaaat ccgcctttta
      961 acacttcctg tacattaaac agacagcagt tcatcgtatc acggcgggca gacatatcgt
     1021 gaatataaat atatccgtca cggcatgcct gtatttcttc cgttgtcatg aaaaatttct
     1081 gatagagttc tttatttaat tcattaaaga tcaggcttcg tttggtagaa accagtgcac
     1141 tgtctgtgtt actgttttcc ttatccccaa tgtacatgat agactggctc ttcttgtata
     1201 cttcgtctaa catctgtaca aaatcctgct tatagttacg gtaatctctg tagctctttg
     1261 ctaccagtgg atttgtctgt tctaatgcac cttcgaccac attatgcata tctgagatcg
     1321 taacttcctt cttgcgcatc tgctccactt tttccgtgac aaagttgcag ataaaatcaa
     1381 gttcttcctc tgtaaatgtt accagtgcac gatatgctga tttattcact gccactacga
     1441 ctttttgtac attaaattct tcttttgttc cgtctttttt aattaccttg atcataattc
     1501 tcccttctct atcagattct ctcatctttc actcattata ttgtatacta gatgtagtat
     1561 tttatttttc ttttacacac tagatagtgt agcttttcaa gtttaacacc gctatctggt
     1621 aaagtcaatg ttaacttttt atctctttcc ctttatctgt aaatccttcc aagagtaaga
     1681 ttcttactat cctttatttc cagttttttt gacaaatatt ctccgaatga gatcgacaat
     1741 tcgtcttctt ttttaaaaat atcagaaaga ttgcgatctg tttttaatac ataaacagac
     1801 ttcgcgatct gaggttcatt tactaattct tttataatct tttcatcttt ttttccattt
     1861 gcaaaaagca cgacttttag atgtccaaaa tttaactgct gacttttata ttgtttccat
     1921 ttcttattcg catcttttaa ggacgttgcc aaaaatgtca ccgaccttga tggtattttt
     1981 gttccagaat cactctgtgc cttgtcgaaa tccgcatacg aataacgaac caatactttc
     2041 ttttcattga gccgttcaaa tcccatcgcc aacacaaacc tatgatcttc cacatcttgt
     2101 tgtttgctgc atccttgtat gcaaaatgca attagcagca ccaaaagata ccctctatat
     2161 tttttcatct ttctgttcct ttctccacat cagaaataaa ataagtggaa aaaacaatcc
     2221 gatcaccaga ttcccataga taaaaacaaa aaaataccgt ctgatccatg ttgcaaatgt
     2281 tgtcatgatc catggactta tgacgtaaat tccaccatat gacaaactca atcccacgat
     2341 ccttcctttt gaaaatgcat gtttgatact ttcatttcca taaaataagt aaccgctgaa
     2401 tacgcaaaag acacctacga tccaaaatgc gatcaaaaaa agatccagtc ttgccatgat
     2461 ccctcctggc attgccactc cctgtgccat agacatcaca ggttctggat cgaccatcgt
     2521 aagcttcttt ccaagggaac ctacagtact tgcaaacacc ccaagaaaaa gtatcatcaa
     2581 acctgcaaat gatcgcatcc tgatcggtcc atctttcata tctccacggt aaaaccacac
     2641 gaactcgatc ggatgcatca gcgcaaatac aagaatactt ccattgagca tctctctgcc
     2701 acaccaactc ctgactgcta actcatccca tgaaagattt gccatactca agatcagtac
     2761 gaagatcagc ggtagcaata caaaccaaaa aattccttcc attactctgc ctctttttgt
     2821 taaacctccc tgattcatca gataagccag aagaagcaat ggtaatccaa taaaaaatgt
     2881 tgatttgtct gggagaagat atttttttgt aagatacaaa atacataaat aaaaaaaagc
     2941 tccattcagc agaaatctca cataataaat gatggctgca aaacgatatc tttttagtac
     3001 attttcaata gaaacccctc ttgctgcatg atctacgatc cacaaataaa ttcctgccat
     3061 cacacttgcc atgaccacca caacaattcc tgaatgatat gatttttgta gtgcgatctg
     3121 tggaagaaac attgcagctg ccgaaaatat ttcaaggatc atgatccgat tcccctgtct
     3181 tgccgtgatc atctgcgaat tatttttcat cctcgtcctc cttctgcctt accctcgccc
     3241 cttcttttgt gaattctgga cggtatttca tccgctttag tggataacga accataaaat
     3301 cctgaaatgc tgtagataat cttcctcttt cttcaaccat tggatgtgca tatggaattc
     3361 cataattctc cagatagaat aaatgaataa atattgccag aaatcctaaa aagaatccat
     3421 acaatcccca aaaggcacac agaatgatca gaaaaaactt catcagacga aacgcactgg
     3481 tgaataactc attcggaatc gaaaatgtag caattgcagt caatgccacc acgatcacca
     3541 cgatcgtact gacaagatgt gcatcaactg ctgcctgtcc aacgatcaga ccgccaacaa
     3601 cacccatcgt tcctccgagc tgtcccggaa gtctgattcc tgcttctctt aataactcaa
     3661 acgataattc catcagcagt acctccacga tcaccggaaa tggaactcct tctctcgccg
     3721 agatcaacgc cagcaaaaaa ggcgttggca acacctccgg atgaaatccc gcaattgcca
     3781 cataaaatcc aggaagtcct atggaaatga tcgctgctat ataccgtaag attcttacga
     3841 atgtcgtaat ttcccaacga ttataataat catcacttgc ctgaaagaac acattgaatg
     3901 ttacgggcaa caataaaacc attggcgaat tatcaagaac aactgcaatc cggccctcca
     3961 tgatactact cgcaacctta tccggccgct gcgtcagctg atataacgga aatggagttt
     4021 tactgtcttt ttccagatat tgctgtaaca ttccactatc aaaaattcca tcgacacaga
     4081 tcttatccat ctgtttctga attttattta aaatgtcctt ctgtaccaga tcctcaatat
     4141 acatcaatgc ataatccgtt ttacttctcg ttccgatctg cttctggatc acttttaacc
     4201 tcgtatcccg tatccttcta cggatcaatg ccgtattcat cctcagactc tccgtaaaac
     4261 tatccttggg accaacgatc gccacttcct gatccgcatt ctgtacccct cttgtcggaa
     4321 ataatttgct ggaaaataag atagcccttg cttctccatc cagaaataaa accgtatttc
     4381 cagataaaac cgctgtcata gcatcttcca ttgtatcgat ccatttccag tctgcggatt
     4441 cgatcacata ttggctgatt gctgttcttt cctgcggaaa agaatcctga agcaacggtc
     4501 tgatgacgga ttcctgaagc atttctgtat ttactaagcc gtccatgtat acaatatata
     4561 tgctgatttc gtggtcgact ccgattttga attgtttttg taccaaatcg ttgcaatcct
     4621 gcatgatttg ggttgtgtat tttatgtttt cttggatatc tggggagatg tttcctttta
     4681 ggttgtgagt tcttgtgtgg aagtggtcca tggggttttt tcctcttttc ttttttggat
     4741 agtatgtggg attttggcag gttatatact tacgatattg gtttgcaata ttttatatgg
     4801 aataaaatgt aaaaaatagt tattgtaaat atatggtttt tctggtaata taagaaacat
     4861 aagtcgattt ataaaaacat agtacatttc aacaaaaaaa caaatcaaac aagcaattct
     4921 taaaattcaa acactgtaac tacagtcatg tataatattg cagttctaca attaagaatt
     4981 tcagttcaga attgaagaat ttatgcacac aggaggataa cctatggcag aaaagctaaa
     5041 gaataaagtt accgatggtt ttcaaaaagg tcatccctac ggagaactcc caccttgtat
     5101 ccatgtaggt atcttaaatt tcaaccagat gataagccca aattattacc ataaattctg
     5161 tctcatggat gagaaaacga aggagatata tagtagaaaa tttcaatttc atatgttaga
     5221 attaaagaaa ttaaagtatg caaaagagaa acaacaaaga aagccattat atcaatgggc
     5281 aaaactgata gctgcacaaa catgggaaga actagagcag gaatcaaaag gtaataaata
     5341 catggaaagg gcgctggaag agatgataaa aatcagtcag gatgaaatgg aaagatacct
     5401 atatctacga gaagaaatgg cagaaagcga tagagtcagt cagatacaaa gtgcaaaaag
     5461 gattggacgt aaggaaggaa aaaaagaggg agaaatacta aaattaatca cacaggtaca
     5521 aaagaaaata gcaaggggag attctctaga aaagatagct gatgatttaa tcgaagacga
     5581 aactgtaatt aagcccattt atcaattagt acaaggaaat ccgaataaaa cagaagatga
     5641 catttatcag atgttgaatt tactttaaag cctacagagc attatggaaa aaattataaa
     5701 tctccgttca taaatacaaa aattaatagc caataacgta taacatacac accgtggtgg
     5761 tttgaatagc cgtccgtgta tatgagaaaa acaaaataat aatataaagg atccagaacc
     5821 attgaaattt tgccgttatt ttgacaggtt cgcagcaagc ttggtgtgct gcctgagcga
     5881 agcgagacaa ggaaagcttg cggataagcg acctgtcaaa atgacgtgca taaatttcct
     5941 ggtctggatc ctttattatt ttgtttttct catatacact ccccacgact accatttcaa
     6001 actacaaatt acaatttaca tcttccaatt agccgcctcc acctgcaatt ccaccatatg
     6061 tcgataaatc ccatcacaag ctttcaattc atccggcgtt ccgttttcag caacaacccc
     6121 gtctttcaac acaacaatct tatcagcgcc atccacagtt ctcattcgat gtgcaataat
     6181 taatacagtc ttatccttga ttaattttga taatgcttcc tgtatcaatg attcattatc
     6241 tacatccaaa gatgccgtcg cttcgtccat caggatgatt ggtgcatctt ttaagaatgc
     6301 tcgtgctatc gagattctct ggcgttctcc gccggatagt tctgagccgt tttctccgat
     6361 catggtatcc catttctgtg gcattttctc gataaagtct tcacagtttg ctaattttgc
     6421 tgctgccata acttcttcgt ctgttgcatc ttttcttccg attcggatgt tttccataat
     6481 cgtgttgtta aatagtgtta catcctgaaa tacaatggaa tatagtgata ataatatttc
     6541 tggatcgatc ttagagatat ccatgcctcc gaccgtgatc gttcctttgt tgatatccca
     6601 gaatcttgca gctaatcttg atacggttgt ttttccactt ccggatggtc cgatcagtgc
     6661 ggttacttcg ccttgttttg cggtgaagct tacgtctttt aagatctgtt catttccatc
     6721 gtaagaaaat cctacgtgat caaagttgat gtcatatccc tgattgctta gtgttttatt
     6781 tccatcctgt tcttcgtggc ttaagatttc atccattcgt ttgcactgtg tgtctgtgga
     6841 gatgatcgcg gataaattct ctaatgctac ctggaatgga tcgtacattc ttgatacgac
     6901 cagcagatac atgaagaatg ttaatgcatc gatttctcct tttgcaaata agattcctcc
     6961 gactaaggtt gtcgttccga ttcctacttt taagatcagc tgtgacaggc atacgaacat
     7021 tgcgttgatc gcttcggata ctacggcgtg tttttcaacg gcctgaatct ttttgtttaa
     7081 gccttccata taggtatttg ttgcgttgta tgattttaaa tctctaacgg tttccagccc
     7141 ttcctgaatt ccatcctggc attccagttt gtatttcatt gcattttcat ttaatcggtg
     7201 tgaaattggt cttgcaccga atacaacaaa aaatgcaact ggtaataccc atacagctgc
     7261 caatgtcatt tttacattga aaaagaataa acagatcgtt acgatcgttg tggaacacat
     7321 ggaaccgaat aactgtggga aaaagtgaga acttgctgtt tccagccatg cacagtctcc
     7381 catgatcgta cttgtaaggt ctgcaaggtc ctttttaccg aaaaatgata atggaatctt
     7441 tcttaatttc tctgccagtg tacgtcttct gactccactt tccacataag ttgcgaggaa
     7501 tgttgcgttg tactggaagt atgttgtaat aataatcaac aatattgaaa taaccattcc
     7561 tccgatatac attggcaatt ttgaacttgg aattccacca tttaaaatat cagatgccag
     7621 cagatataga agtccgactg gaaccataaa tgctaagttt gcaattgtta cgatgatgac
     7681 cgctttaatc atatcattga caccctgttc agaaagggca tatttatgtt ttaatctttc
     7741 tctcataatt ataatacacc ccctactttc cagttcactg atttgttata ttcttcccac
     7801 atatgggcgt aaatgccgtg actctttaat aattcgccgt ggcttccttc ttctgcgatc
     7861 gctccgtctt tcaaaacata gatacggtct gcatttgtaa cggttgataa tcggtgggcg
     7921 atcatgatga cggtcttgtc ttttgataag ttttcaaacg ctttctgtac tttctgttcg
     7981 ttatctggat ctgcaaatgc agttgcttca tcaaggatca gaactggagc atttttaaga
     8041 aatgctctgg caattgaaag tctctgtgct tcaccaccgg atacatgaat tcctttggct
     8101 ccgatcattg tatggacgcc gtctttaaat ttgtcgatga tatccataca ctgcgcatct
     8161 tttaatgcct gcataacttc ctcgtcggtt gcatctggtt ttccaagtct gacattttca
     8221 aggatggaca tctttaataa tttgctgtcc tgaaatacat aagaaatctg attcataagt
     8281 tcactgcttg tgatatctct cacatcaacg ccgccgatct ttacgcttcc ttctttgaca
     8341 tcccagaatc ttgcgatcat ggatgctaag gttgttttac cgccgccaga aggtccaaca
     8401 aatgcgacat gttctcccgc tttgatctca atggaaatat gatcgactgc atttttcttt
     8461 gccccttcgt atgcaaatgt tacatcccga agagatacgg aactgtcttt tggaatctgt
     8521 ccgttggttg tctcttttaa tgactgtctg ttcatcacat cacccatacg ttttaatcca
     8581 tcaacgacct gcatctgggc ttcgcctgcg tatgcaagtt tcattaatgt tgttgttaaa
     8641 attggtgtaa tgatgatata gaataataag tttaacccaa atgctgctgt gatctcatgt
     8701 ccgccaaata cataggctgc aatgatgata aatacaaaaa tggaattgac cgctgttgta
     8761 aatccaacca ttggttttct catttttttc gtaaatccca gtgtccattt ttcatattca
     8821 tcaattgctt ctttgaatct cttaaatgaa aataccgtct gtccaaatgt tttgacaacg
     8881 ggaactccac gaatatattc aaccgcttct gctgacatga cttccaagga attctgatac
     8941 tgtttcatac tttctgccag atcttttcca ctcatggaac tcatgaatac gaatccgatc
     9001 gctgccggga tcagacacat gattcctaac ctccagtcaa agactgccat taagatgata
     9061 agtccgatcg gtgttgcctt ggaaactgct ttatctggaa gggtatgtgc cagatatgtt
     9121 tctgttgctg cactggaatc tgtgacgatc tttcgaatct taccagttcc ttcgctttca
     9181 atatatccta atggtaatgt catgatgtgg ttcatcattg ctgttctcat ctgtgcctgt
     9241 acatggaatg ctgcgatatg agagcacatg agacccgcca tatagatgat cattccaaga
     9301 atcgcaaatc ctaccgcacg ccatccataa gtgctgattc ctgctgcctt tgagaaatca
     9361 aatctgactc ttaacacttc ctgcatcact ttccaaatat aataaaatgg aactaacgca
     9421 acaaatgcac tgatcgctgc aagaatggtt gagagaatgc ttaaatattt gtaactgcct
     9481 gcgaaatggt ataactgctt tgtcaggctt tcttcttttt ctttcttttc ttccattata
     9541 ttcacctccg taaattattt attgtcggac aattccatct gtctgctgtc cttatttaaa
     9601 ttgtgcttgc gcttatattc aagcggtgca gccccgtaat aattcttaaa tgcttttgca
     9661 aatcttgatt gtgtcgcaaa tcctactgca tcactgatct ctgatatttt cattgaattt
     9721 ttcagaatca attctgctgc ctttttcatt cggatctcat tcaataattc gctgtatcct
     9781 cttccatata ctccctggaa ataattctta agacttgtct cactgattcc aaaatgtttc
     9841 gcaagtgtcg ctgcagaata tcgtttgctt aaatcttctg tcagaatatc catggtcttt
     9901 ctcgcaatct gaatctgtga acctgagtag tacaccctgt ttgtctccac tgcatccctt
     9961 ttgctgcaaa gaacctgaag ccaccggctg acatccaccg caagaagatt catatctgca
    10021 tctgcttcaa tgtcatcttg aatcactctc actgcctgtt caattcttgc atctgctttt
    10081 gaaataaatg gtgtctctct tcccagaaac cgttctttta attcaacgct cattttggta
    10141 ttcctgccat ttaatgacag ttctttgtca agttctggac atggatacaa gatcaactcc
    10201 attccatgat actgtgctct tggatagtaa aattcatccg cttctcttga agcagtccca
    10261 tagtcgattg aatattcccc ggtatccaca taagtaccgc caccattttt caaaagaacc
    10321 tcgcacttgc caaggaaact gaaattcact gtcatgaccg cttccccttc gtaggcctga
    10381 atctggactt ctgggattct tcttgatctg atatcaatat acaaaagctc cagatatgag
    10441 ctgatccgat aacagacgat ctttcctttc tctcctgaaa aatctgatgg caggattact
    10501 tctcgtttct cattcatgaa aaactccttc tctcttcctt tcttttataa tttacggtta
    10561 tatgttagta tacactaact tttgattcaa gaacaaaaca gcaaaatagc tccttttagc
    10621 atctggtttg ttcttgattt tgtctttttg gtaaaaagaa tcctgtttta tggaatctcc
    10681 tgcaaacaaa aaaaggaaag gatcatcaca aaacatgata atcctttcca atttgtttaa
    10741 aatttcaaat tctaaatctc attattttct aataatatca gttgcactaa agactttatc
    10801 acctttgcct gctttcacac ccttagcttc taacagctcg gatactgtac aaacttcata
    10861 tcctttcttc ttaaggatcg gtaatacttt ctctactgct gcaacggttg gcttatgaat
    10921 atcatgcatt aacacgatat ctccgtcttt tgcatgtttt aagattgttt taacagtcgc
    10981 atctgtgttc tttgtcttcc agtctaaggt atcaacactc caaaggatca gtggtgtatc
    11041 aattcctttt ttaacattct cattgatcgc tccatatgga ggtcttacgc tgactggttt
    11101 acttccacat gctttgtaga ttgcattcgc tgtcttgtct tcctgttctc ttacctgtgc
    11161 agcacttaac tttggaaggt atgtatgatt ccatgtgtgg cttgatactt cattacctga
    11221 ctctaagact ttcttgactg tctctggata ataatttgca ttctgtccta acatgaaaaa
    11281 tgttgctctt gcatcgttct tctctaatgc tgctaatact ctttctgtat ttccttttaa
    11341 tggaccatca tcaaatgtaa atgcgatcag tttctttgtc ttatcgatct ttcttggaac
    11401 gtagagtgat ttcttcaatt ctttgtctgt tcctttaccg agcagatcat catttgcctt
    11461 taccatgtat tctcccatgg atttgttcac tgatgcataa cttgttcttc caaatcttgt
    11521 cgttacaaaa aatattcctg ctgcgatcac caggataatg atcaatagtg ctgcaactct
    11581 tcctttgttg atgcgtcttc tttttcttcg tcttgccatt gtacccttct tctttcctta
    11641 tgattttaat aattgaaatg tatctacatc cacaagtcca aggtctgtca gtcttaatga
    11701 tggaatcact ggaagtgcca taaatgataa tgtgataaat ggatcaatat ctctggatat
    11761 tcctagtttc catgccttct gtaacagctc atcgatacct ttctggatgt ctttcgccgg
    11821 tttctggctt aaaagccccg caaccgtcag cggtaaagta cctaaaactc tgccttcaga
    11881 taccagtgcg tatcctccct gaatctcctc tatcgtctgt acagctttga tgatatcttc
    11941 gtcattatct cctgccgcaa tgatgttatg tgaatcatgt gctacagatg ttgcgatcgc
    12001 accgttggtt atcccatatc ccttgatcgg tgccgcagct gcattgcctg ttccgcggtg
    12061 acgttcaaat acacatagtt tagaatattc actatctgga gtaaataacc catcctttga
    12121 tggaacctct tcctgtaaaa acttcgtgac gatctgccct gggatcattc cgatcacagg
    12181 gatctttcct tctgctttta cctgaatctt atctttggag atattcttta atacaacggt
    12241 gtgaagcatg gattcattca ctggttttgc ttcttttgta aagaattctg tgttcacaat
    12301 tcttccttgt ttgtatacag aatgtacttc gacactctcc aaagaatcaa ggatcacgat
    12361 gtccgcatct tttccctctt ctaatactcc gatatcattt aaaccatatg tctttgcagc
    12421 attatatgtt gccatcttta ccgctgatat tggattcatt ccaagctgga tactcttctt
    12481 tatattgtaa ctaatatgtc cttcattttc aatagtatca agatgtttat catcggtaca
    12541 gaacataaaa tgtgcaataa attctggttc tttgacaagt cccggaacta tattttctac
    12601 attccttgct gcacttcctt cacgaactaa gattttcatg cctgcaagac atttttcttt
    12661 ggcttcttca aaagatgtac attcatgatc tgtcataact cctgcttcca catatgcctt
    12721 taagtcttct ccgctaagtc ccggtgcatg tccgtcaatg accattcctt tacacagctc
    12781 taatttcttc atgatctgct cttctttgct tagaaccgct ggataacaca tcacttctcc
    12841 aagtcctaag acaagcggat ggtccttcca ctgcttcata tcttccgccg taaagtctgc
    12901 cccatttgtc tcaaatggtg tcgctggaat ggatgaaggc atcatgacat agattccaag
    12961 tggggcatcg cctgctgcat ccagaatgta ttccatcgct tcatttcctt tgacattgac
    13021 aagttcatgg ggatctgcga taaccgtcgt tgtaccatgc ggcataactg ctttggaaaa
    13081 ttccattggt gtcgccatac tagattcaat atgtacatgt gcatcaatga atccaggtgt
    13141 gatatatttt cctgtacaat ctattacatt ctcccctgaa aaatgaccaa ttcctgcgat
    13201 ttttccatct ttcactgcaa tatctgcctg aaggatctgt tcatttttca catcaacgac
    13261 atttccattt ttaaagatca catcatacat cattcttcct ccttaaattt tacgattcct
    13321 acataatatt tcttatctgt atcaaatagc atattccagt cgatttcttc gcactctcca
    13381 caattcgacc agatcaattt ctcttcttcc tcatcaaatc ctgtgatcgt cacccagtgc
    13441 cagttatctt ctcttaattc atgcgccgga tgtcttaaga tcagaaatgc aactggatgg
    13501 tcttccttta atgctggcaa gataaaatcc aatgcctgtt ctctactttt gggtaaaaat
    13561 agctgttctg aacttaatgt gattccatga tcttttgcat atcttttgaa atctttcgca
    13621 aatctcccaa tcagtggata tcccataaat ccaggtttca tatagcgata catcgtctgc
    13681 atcaaattta aatattgttc atagtctgct gctttctgac tatcttttat cggatataaa
    13741 tctttaaaat cttcccgact gagtgcataa atagctgcaa tattgctgcc tgtcacactt
    13801 ccacatcctg ccaggcgctg aaaatctcct ggaaaccatt cctgatcgcc gccatatcct
    13861 atggttttac cgtcctttat gatctgtgga cagtgaagtt ctttcctcat agtctgtcct
    13921 ttctgttata ctcttaaatt cattgaattc ttttatttct ttatgttaag attgttatca
    13981 ttagcagata tgatcattta caatttacag aaaggatgag aacatctatg aaaaatatcc
    14041 tgatatcctt atgtcttgca ctatttatca tcagtgcatc cgtaacgata acattaaact
    14101 tccgaccttt atattatcat gatatttcct ctttgaaaat agaagaaact tccggttttt
    14161 ccaaaaaagt gattcgtgaa aattatgatg ctttgattga ttataatcag ttcttttatt
    14221 ctggcaaatt aaaactgacc ttgcctatgt caagagaggg caagattcat tttgaagaag
    14281 taaaacgaat ctttgtcggt atcgaatgcc tttgcgtgat cacactgatc ttatcttgtt
    14341 tcctcataaa gagaaaaata aggtcacgaa atattgattt tttgagatca gcttctatta
    14401 taactgtttt attacccgta attgttggta ttttatgtgc gttcaactgg gatgccgctt
    14461 ttactttgtt tcatgagatc atgtttcgaa atgattattg gatttttgat gaagctactg
    14521 accctgtgat catgatcctt ccagatgcat ttttccttca ctgcgccgtt atgatcatct
    14581 tactaatctt aattggcagc ttactttgta tgggatttcg ttcttttttt cgacacaggt
    14641 ttatgctata atggatagat attaattatt tgtttcgaat gttacatttt tattaagcta
    14701 ggagtttagc aatgagactt tcaaaaataa aaacaaagga aatccgatat gtagtctgga
    14761 aataccgatt aaaacgattt cttccacata tcttaacact ttgccttgct gttattgttg
    14821 ctggcggagc ttttagtgct tattcggtat atcataaaaa gcatcaaaag caactggcaa
    14881 aacaatctga ggtccagcgt gaaaaagatg ttaacactgc tcgtaaaaag gcacaaaaag
    14941 aaaaaacaac actgaaacct tggtatactt accacagcat tgcccatgca atgggcgggc
    15001 ttgatggcaa agattatttg aattccatcg atggctttta ccctgcttat cagaagggtt
    15061 atcgtgtctt tgaaatggac atccttctta ctaaggacaa cgttgcgatt gggaagcacc
    15121 aatggggtcg gaaactttct gatccaacca gtaaaaaagg cgacccagtc agttaccgta
    15181 aattcaaaaa tactaagatc tatgggaaat atacaccaac ttctttcctt gatgtattga
    15241 atctcatgga aaaatatcct gatttctatc tgatgacaga ctcaaaaagc acagagcctg
    15301 cagatgcaaa aaaggaattt aatgttcttg tacagacagc taaaaaagtc ggtaaagaag
    15361 acctcttaga ccgtgtgatc gttcaggtct acaatcagag aatgtactgg gctgtaaaaa
    15421 gcgtgcatcc attcaagcat tttgtataca ccacttacaa acagccagat gcagcatttt
    15481 ataaagtcgt aaagttctgt aaacaaaatg gtattgaagc gatcacttct cctaagaatg
    15541 atatcaatga ttatcgtatg gaactgcttg caaaacaagg aatttattca tatacacatt
    15601 ccgtgaataa tgcttatttt gcgaaagagt ttatgaaact tggggtttat ggagtatatt
    15661 ctgattttct tagcccagca caggtgaata atagttatat tagggcgaat tgtccgaagt
    15721 ttgcttcacg gtatgtgaag actattatgc cgggcatcaa tcagtaaatc ctgtaggtag
    15781 tctttagtat gaagactatt caaaccacta cacaatgtgt atgttatatg atatcggtta
    15841 ataatttttg tatttatcaa ctacttcgta gatctgacct tgaattaatt tctatgaatt
    15901 atcaatcaca tactcatcaa ataatggtaa tgtaaagtat acatatcctc tcatttcacc
    15961 tgacaagatt ccctttttta tcaaacgttt tcgatatgga ttaaattcat tcgtctccat
    16021 tcctaaacgt tcccggatat ctttgatctt cccaccatca acctctgcaa ttcctcttgc
    16081 tactatccta tctttctgtg ataattctga ccagatctta tcataaacaa attcactcaa
    16141 atactgttca tattcatcaa tgactgcttc gtaatcaccc tcatgattcc atgttaaata
    16201 tccaagaacc tgaaatgcaa atggataacc ttttgtaagt tctgccatct tcgcagcttg
    16261 ttctgattca attttaaaaa ttgttttata tttattcatg attgcccgat tatttaacgg
    16321 ttttaattgt atctttggtg ctctatataa gaatgtaaga ttcttttcat tctgtaactc
    16381 atcgatgttc tcatataaac ccgttgctaa taaaaatact ggtagatcct gccttacaaa
    16441 aatctgaaaa gatccagcaa aaacctgcat aaattcatta ttcgtgactt catcaattgt
    16501 gatcaagagc ctcttcccat tctttttcat cttttccaga attgctatga tcgctgtctc
    16561 ttcatctgta attggtgaca ttccttcaat tgctactcca aaaccgaaaa atgacagatc
    16621 aattttggca gatttaatga ttccagcaca taccttgttg ctatttaact ttgataaaag
    16681 tcctttcaat aaatctgttg ctggattcaa ttctattacg atccattgat cattttctct
    16741 caatttctgt gaaatctcag tcatcattac agtctttcct gacccacgca ctcctgtaat
    16801 aataaacatc tgctgattga tctgttctgc tgtaaatgta tcaatgatct ctttcgtctg
    16861 tactggacgt tccactggtt ctaacggact ttttccaaat gtaagtgtaa aaggattttg
    16921 catttcttat cacctcttat ctcttttact gttctttact gtttttatac tattttactg
    16981 ttctttactg tttgtgtcaa catcccaact cttgcagcat tatgatcaaa atttaagcct
    17041 catctgattc cacttcactc ctccatgctc agactcagat actccctcat tctcaaatcc
    17101 aaatttcgca taataatgca ccaaatgatc cttacaagta agcactaatc cttttctttc
    17161 ctgctttctt gcatcttcga tcgcacattt gatcaactct cctgcatacc catgttttct
    17221 gtatgcaggt atcgtattca ccccaaagat catctgccac gctccacttt cattgtgcaa
    17281 ctgagctttt gcatacatct catctgtcag atcttcttca tccgttacaa atccatctac
    17341 aaacgcaatt aatttatctc catcaaacat cagccagaaa tgatcagcat agtattttaa
    17401 tcgttctgct aattcttctc ttgttgctgc ttctgctggt ggaaagcatt ctgcctctac
    17461 agatgttact gcttctagat ctttgattgt tgcggttctt atattcatat tttccccttt
    17521 tttgtttctt gtagtttatt atatgtattt attttaacac ggtgttattg tatgatgtaa
    17581 gtataaataa gtggggaaaa cattttggaa taggaggaaa gtaaaaagta tgaaaaagat
    17641 aattataggc gtgatgatcg taatttcgtt tgtatttgtg ctaaaaatca gttgtgttca
    17701 agnnnnnnnn nntttattta ttttttatca tatactcaaa atctacagtt ggccaactac
    17761 aaaaattaaa atttaatact cttccttaat attacggatc actgtcattt cctgatttgt
    17821 aatatgtttc ttcatgatct tgcgtgcttc ttctttctta cctgctctga tcgcattcac
    17881 aagctcttta tgctcattca acaatacaat atggtaatct gtatccttta catattccag
    17941 acgataacgg tagaactgtt ctcttaagtt tccaatgatc tgaatcagtc gtggattccc
    18001 tgttgcatcg aagatgatgt catggaattc catatcgctg tctgcgatgg ctgcattatc
    18061 tccagattcg attgctgatt cgaattctac taatgcctga tctaacttct cctgtccttc
    18121 ttcatccata cgttccgctg caagttctgc tgctaattct tctaaggaac aacgtacttc
    18181 taagacatct cttaaatctt tttctgtgat ccttgcaact tctgctccct ttcttgggat
    18241 catgactaca agtccttcca gttccagttt acggattgct tctcggacag gtgttctgct
    18301 gactcctaat cgattcgcaa gtgcgatctc catcagacgt tctcctggtg caaattctcc
    18361 tgtgatgatc gcctgtctta atgtattaaa taccacatct ctcaatggca gatattcatt
    18421 catgtttacc tttaatttat cgctcatagt ttcttctcct cctgctttct ccaatgtatc
    18481 ttccatactg ctttcttatt tgtgaaaata cattattgta tcggtcttac tacgtatgcc
    18541 tgtttgatgt cctcaatact tcgtaatgat tccattgcat cgtccgctgt tttcttatcc
    18601 tcaaagattg cgaagattgt tggtccactt ccactcatta aagctccctt tgctccctga
    18661 tccatcagat gtttcttaac tttctcaatg atcggatatt ccttgatcgt gactgtctcc
    18721 agtacattct ccagacgatt acagattcct gtaagatctt tctctttcag gcactccagc
    18781 attccatcga tatccggatg cttcgaaagt gctggaagat caaggttctt atatacaaat
    18841 gctgttgata cactgatccc tggttttgcg atcagaatat agcaatctgg catcgcatca
    18901 acccttgaaa gtttctctcc gattccttct gatagtgctg taccacccca gatacagtat
    18961 ggaacatctg ctccaagcgt tactcctatc tcacataatt catcgattga taatcctaga
    19021 tcaaacagct gattcattcc ttttaatgct gctgcgcaat tagaacttcc gccagccata
    19081 cctgctgcta ccggaatccg tttgctgaga ttgatctcaa ctccatcttt aatatgatac
    19141 ttatccttta tgagttttac agccttataa acgagattac gttcatcggt tggcagaaac
    19201 gaaagattac aggtcatctc aatcttgtca tctttccttt tcttcattgt aagcacatca
    19261 taaagtccaa ccgtctgcat gatcatacgc acttcatggt atccatcttc gcgtcttctt
    19321 aaaacatcca gaccaaggtt gatttttcca tatgatttta gaacaatact atgatccata
    19381 tgcactcctt tatcttatgt gaaatagttt ctatctcaca ccttgtatac agaatattat
    19441 agtattgttc tgaaaaaaat gcaatcctta tttgctaaaa tattttcatt tcttttacga
    19501 ttaaaagctt atctcctggt ttgatatcgg aaatgtcctc attttgctcc cgaatgctct
    19561 caaccgttgt aaaatacgcc ttggcaatag accatagagt gtctccttct ttgacgatat
    19621 acccaacgat ccctgggatc tcacgtattt tgtcgtagtc tagcggtcct tgcttcatat
    19681 ccgtcataat ccgctgtctg actctgtgaa aagcgataaa atcaaataaa acagaagctc
    19741 tgatctctat ctcttctcct tctgttggaa tcgctgtcat ctgatctacc cgcacatcca
    19801 gctgatagtc ctcatttcca gtcagattct tcgtctcaac aaaaaatgta aacggctcca
    19861 tataagaact gctcatcact ggtgtcatat cttctgtgga cagatacaag acatctgcca
    19921 tccacacacc ttctacagat aatccttgtg ctgtcatttc cacatcgtct acggttgcac
    19981 ttcctttaac ggataacact tgcaacagct ttcctgactc ttgttccagc cgaaagcgtt
    20041 tgtctgcttt catcaccgct tggttctttc ctaccagtgt ctcaaaatcg aacatatgat
    20101 attctggaat tagcgtttct tccatactat atccgtcatc aatatatgaa atcttatgat
    20161 cttcataaat tttaatatca cagtcgatcg taacatccaa agaaataatt ctttcttctc
    20221 catcctcatc aggtcttatc tccaactgct gtcctccgag tgtcatcccg atccgtccga
    20281 tcattcctgg catacattca taacattcca gctgcgtatc taccggaatc tcccatctcg
    20341 catactgtac cggcatctgg gcatcttctc caagatataa tacaaatata tgcattgcac
    20401 cctgaatctc gatcatgtgc tctccaattt ttgcctgcag attttccata tctacctgtg
    20461 tccataaaat ctgataaatg ttcgccttat ttgcaggaag taccaattct tcttttaatc
    20521 tcgcgatatc ctttttattt acgatcagat ctgtgatctg tacatctttc tttaacacgg
    20581 atacattttc ttcatgaatt tcaatgatcc ctttttcttt catttcttcc gtgatctgaa
    20641 aggaaaacgt caataataca cgaatgctta ttttccttga attgatcaaa atcgtattga
    20701 tgtcatcgat cgtatatttt acttttacat aatccgatgg gagcactcct tctatatgta
    20761 catattcctc aaacggaagt gaatattcca gcgattcaag gtatgctgac gtatcatttg
    20821 taccatatag cccctgaaat acaaattccc ccttgatcct tacccggtca accatcattt
    20881 ccgtttcctg tatctttacg attcctctgg tctggatcat cttttctaca tctggttttg
    20941 tatctgcaac attgcagtcc tgatcgatcg tgatctgcat ctgtttcttc gctttcgcca
    21001 cgatcccata aaattctttc ttatcaaaat ccataaaaaa attcctccct gtctgtatat
    21061 acaaatatat tcagacaaga aggattctat gcatcaccca ttagttttct aataacatca
    21121 tacggacatc tcttgtgatc acgtcctggt agctgaaact tacagtttta ttctggtcat
    21181 tgccatctgc attctctaac tctaccaaaa agatattagg atatgtctgt gtgatcactc
    21241 ccttagctgt tacaaattta tgtcgtcctc tgtttgcact aatcttgatt ttgctgccaa
    21301 tctgattctt gatggattgg cgtactctgt taagatctaa ttgtgtcata aattcacctc
    21361 ttccatatac agaatattct ggaaattcta tataatgatt ttaatattat aacattttgc
    21421 ccagtttttg tcaaatttat gcatacaata tccagtttta tcactattat taccaaacaa
    21481 gaaaactgta caccgatgag gcgtacagtt ttcttaacat attatatgga gtttattttt
    21541 atatggagtg ttttaactat tatagacatc cttgtctaat tcattttctt tatagctgac
    21601 gatcactgtc gttgccgcat ccaagttctt ccatgcatgt ttttctgttt aaaggaagtg
    21661 ttgctgcgtt agaagttgtt gtttctagaa gattcacaga gaaccatccc atggatttga
    21721 taaagtatgc aaacaatcca gctaatgttg caccgcatac ataaaaccct acaaatgttc
    21781 caattgtctt tcctgcgatc tttccaagtc tctttggatc tgccatgctg cataatgcaa
    21841 ggcttaatga tgctaaaaca attggtgcga ttgaaacggt agcatcacag actatcatat
    21901 cttcttaata aatcaac
//
LOCUS       DAOMUE010000007        20371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_435_length_20371_cov_2.937537, whole genome shotgun sequence.
ACCESSION   DAOMUE010000007 DAOMUE010000000
VERSION     DAOMUE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 20371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20371
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_435_length_20371_cov_2.937537"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLC71_00875"
     CDS             complement(<1..342)
                     /locus_tag="PLC71_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM01230.1"
                     /translation="MFQSDFGIIADYFVKRRKGYKTIENHKPIKHADEMLKFIRIFAE
                     DERFLKLNLEKDKKGAITMCTILDAVEGRGIEKGITQGETLKLIMLVQKKARKGDSIA
                     KIADDLVEDEIV"
     gene            557..1591
                     /locus_tag="PLC71_00880"
     CDS             557..1591
                     /locus_tag="PLC71_00880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRM01231.1"
                     /translation="MTLEEEYRLSCYEELTTLGNHNHIYLIKHKLSGEIFVKKVLSRF
                     SLDVYKQLRDLQISGIPKIHDLILENNSLILIEDYIHGQTLEQLLEEQTTLVYSDALA
                     IMRKLCDVLKSLHTLTPPIIHRDLKLSNIILTSENLVYIVDFDTARYYESGQEQDTEL
                     LGTKEYAPPEQYGFGQSDARSDIYALGIIFNRLLTGEYPKHQLADDRYRSIISKCIRW
                     NPEERFQTVEELKTALHDNISSDIGKPGFTLSSIHSDIPGFRTGKLWKMILAFFGYLS
                     FLYCSMTSDFTNAKTGLPLTGLQLWHERFFVFLMLLSLIFIILTTKISEPDLFWVILS
                     LLYFELFYSV"
     gene            1759..2103
                     /locus_tag="PLC71_00885"
     CDS             1759..2103
                     /locus_tag="PLC71_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01232.1"
                     /translation="MKTWKLVSGIISIVLFAFVMFQSCAAGISNSLAENGESGGSAGL
                     IVAIILLAGGIVSIATRNGSKGGNIALIVLFALGALCGFTMAGSYADLNVWAAWCLIC
                     AVFAAVSLKKGQ"
     gene            2144..2590
                     /locus_tag="PLC71_00890"
     CDS             2144..2590
                     /locus_tag="PLC71_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM01233.1"
                     /translation="MQDQLTDHLDQQLKSTSLENFSNYLKNLQQLPSLQDFFSVYIAK
                     HDLKISQIIKNSGISQSYAHEILNGTKPHPSRDYLLALCLGAHMDLKTTQHALRIAQL
                     GELYAKVPRDAAIMMHINNEKWNLIDINIFLEEHGLNVISLSKKIS"
     gene            complement(2734..3417)
                     /locus_tag="PLC71_00895"
     CDS             complement(2734..3417)
                     /locus_tag="PLC71_00895"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM01234.1"
                     /translation="MNQQHRFIFWGVLLIVVLLSVSSITFPIFVAQTVKNQNVICIDA
                     GHGGSDPGKVGNSNILEKDINLSLALKLQKLLEKKNYKVYMTRNGDYNLADSKENEKR
                     SDLSNRKQLIFENDPMAVISIHQNSYPSSDVHGAQVFYPQGSEKSKQLADHIQSALIK
                     GLDPNNHREAKANAEYYLFRDNPYPIVIVECGFLSNPEELKALQDDGYQKKAVQCILD
                     GIESYLSDI"
     gene            3800..4852
                     /locus_tag="PLC71_00900"
     CDS             3800..4852
                     /locus_tag="PLC71_00900"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRM01235.1"
                     /translation="METRLVKINNMEDCKKDMEEAGELIKAGELVAFPTETVYGLGGN
                     GLLPDAAKKIYAAKGRPSDNPLILHVCDMQMVESLVKEIPDNARRAMEHFWPGPMTVI
                     LQKADQVPDCVTGGLDTVAIRMPDHPVALELIRRSGVPIAAPSANTSGRPSPTKAEHV
                     MEDLHGKIPMILDGGAVMVGVESTIIDFTETVPVILRPGWITKESMEEFLGCEVLMNT
                     SLKASDHGIPRAPGMKYKHYAPKAEMVLVLGDDQEKVVDHINQLAKEQKEQGKKIGII
                     ASDETKDLYQADEVVSVGQRAHLETVTAHLYDVLREFDHKDVDMIYSEGFEGEPLSEA
                     IMNRMVKAAGHEILRI"
     gene            4866..5750
                     /gene="rpiB"
                     /locus_tag="PLC71_00905"
     CDS             4866..5750
                     /gene="rpiB"
                     /locus_tag="PLC71_00905"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRM01236.1"
                     /translation="MKKIIMVSTNNTCRSFIAESILRQYLLDADQKNVEVISRGLVVL
                     FPEPVHVKAADMVIKTCGIESLDFRSAQLTQEEVEESDLILTMTEEQKDKILEEYHGY
                     KEIATLNEYARVEGAIIDPYGMEDDDYERCFEQIARLVKKIWQERLGGNKMIGIGSDH
                     GGYALKQAVIKHLEEKGYAVKDYGCYSEESCDYPVYAKAVANAVKDGEVKQGILICGT
                     GIGISITANKIKGIRAALCSDTFSAHATREHNDANILAMGARVVGEGLALDIVDTFLE
                     TEFSNDERHIRRINMIED"
     gene            6206..6889
                     /locus_tag="PLC71_00910"
     CDS             6206..6889
                     /locus_tag="PLC71_00910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRM01237.1"
                     /translation="MGSLGDYLMEELSLKTSFTIPIGGGIPVSQSAVTTWVIMAIVVL
                     LCIIFVRNLKVIPEGPQVYVEALVGFIYNFIGGLVGEKGKRYIPFLGTILIYLGCANI
                     SGFFRAKPPTKDLNVTAGLAIISLFLIIYSGIHEKGLKGYIHSYVEPMPMMAAINILE
                     LFTRPLSLCMRLFGNVLGAFVLMELITHIAPAIVPLPFSCYFDFFDGLLQAYVFVLLT
                     SLFISESIE"
     gene            6945..7163
                     /gene="atpE"
                     /locus_tag="PLC71_00915"
     CDS             6945..7163
                     /gene="atpE"
                     /locus_tag="PLC71_00915"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRM01238.1"
                     /translation="MGLAAIGAGIAVLTGLGAGIGIGVATNGALGAIARQPEEAGNIN
                     KTLLLGCALAEATSIYGLVVAILLIVMK"
     gene            7182..7664
                     /gene="atpF"
                     /locus_tag="PLC71_00920"
     CDS             7182..7664
                     /gene="atpF"
                     /locus_tag="PLC71_00920"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRM01239.1"
                     /translation="MFLKLDWGIIWPIVNIVVFYLLLRKFLFGPVSEVMEKRKKMISS
                     DLDDAAQTKAEAEEIKQEYEKNLAQAKDEAGQIVSDARARAKNEYQNKMDQTKEEIAL
                     MRENARKDIEAEKQKTIAGLQTEIAGIALMAASKVVEKEANDKGNEKLLDDFLKEAGV
                     "
     gene            7668..8171
                     /gene="atpH"
                     /locus_tag="PLC71_00925"
     CDS             7668..8171
                     /gene="atpH"
                     /locus_tag="PLC71_00925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRM01240.1"
                     /translation="MKRISMTYGQVLFELGIKKESLQKAQDMLHENEELLSALENPTI
                     TKKEKENVVEKLFSDDIKSFLKVVCDNDDIACFDEAVEYYDELKRKTDKIIKAEFDYV
                     TMPKDEQLERIKQYLMKQYQADKVELTLKEEKDLIGGFVLKVGDHVYDNSLSGKMRKL
                     QQKLTWR"
     gene            8179..9678
                     /gene="atpA"
                     /locus_tag="PLC71_00930"
     CDS             8179..9678
                     /gene="atpA"
                     /locus_tag="PLC71_00930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRM01241.1"
                     /translation="MSSINPETVVSVLKDQIENYDQKTEVKEEGRIIQIADGIATVYG
                     MDQAMYGELVIFETGVHGLVLNVERNCIGCVLLGSDHGLMEGSKVARTGVQADIPVGD
                     AMIGRVVNAIGEPIDGKGEIKTDERRPIEHEAAGVIDRKSVDQPLQTGILAIDSMFPI
                     GRGQRELIIGDRQTGKTSIAVDTILNQKGQDVICIYVAIGQKASTVSQIVTTLSKHGA
                     MDYSIVVSASASDLASLQYIAPYAGTAIAEHFMHQGKDVLIVYDDLSKHAVAYRAISL
                     LLDRPPGREAYPGDVFYLHSRLLERSCRLSDELGGGSITALPIIETQAGDVSAYIPTN
                     VISITDGQIFLESELFFSGVRPAVNVGLSVSRVGGAAQTKIMKKVAGNLRIDLAQYRE
                     LEVFTQFSSDLDDGTKATLNYGSHLIELLKQPLYHPMALHKQILLLFAAGHKMLNDVP
                     VKELKAETEEMTAFFEQKYPETVKKIDEGKVLTDDIAKEIQEAIEAYLK"
     gene            9688..10557
                     /gene="atpG"
                     /locus_tag="PLC71_00935"
     CDS             9688..10557
                     /gene="atpG"
                     /locus_tag="PLC71_00935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRM01242.1"
                     /translation="MANIREIKTRMNSIEDTLKITNAMYLISSSKLKKARKQLDATTP
                     YFEMLQASLLDIMAHTPDLQHIFLNKRKKEHPKKGYLVMTADRGLAGAYNHNITKMAE
                     ENINEDDKLFVIGYMGRNYFGRRNAAQMDEDFFYTTQDPTIYRARRIAEKMIEEYVSG
                     ELDEVYIIYTKMITSITSEPKMIRVLPLNPDQYEFAGKGKRSHMTEFQPSVKAVLDRL
                     APNYIKGLVYGAMVESFSSELNARMMAMEGASKSAREMIRSLGLMYNRARQAAITQEI
                     TEVCAGAKSLKKE"
     gene            10579..11967
                     /gene="atpD"
                     /locus_tag="PLC71_00940"
     CDS             10579..11967
                     /gene="atpD"
                     /locus_tag="PLC71_00940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRM01243.1"
                     /translation="MEKGKIVQILGPVIDVEFETEQLPLIHDALTVVNKGKEVVMEVE
                     QHMGNRTVRCILLAASEGLHKGMEVTATGGPIKVPVGKQTLGRMFNVLGEAIDKGEPV
                     ESEEKWAIHREPPKFEDQSPVVEMFETGIKVIDLLAPYAKGGKIGLFGGAGVGKTVLI
                     QELIHNIATEHGGYSIFTGVGERSREGNDLWNEMTESGVIKNTALVFGQMNEPPGSRM
                     RVAQTGLTMAEYFRDQEKQNVLLFIDNIFRYVQAGSEVSALLGRMPSAVGYQPTLANE
                     VGALQERITSTKNGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRAISEQGIYP
                     AVDPLESSSRILEPDIVGEEHYEVARRVEEALQRYKELQDIIAILGMEELDEEDKITV
                     YRARKIQKFLSQPFNVAETFTGIPGKYVPVKETIRGFKAILDGEMDEYPEQAFLMVGT
                     IDDVKEKAKEVM"
     gene            11968..12381
                     /gene="atpC"
                     /locus_tag="PLC71_00945"
     CDS             11968..12381
                     /gene="atpC"
                     /locus_tag="PLC71_00945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRM01244.1"
                     /translation="MAETFYFEIIASDKKFYSGACEHVIFPAVDGLYGVLANHEDTVT
                     AVVAGELRFKVDGEWKVCIVGEGFADVTRDFVVIVVDTVERPEDIDIIRAREAKTRAE
                     ERLKQKQSRLQYYHTQAALSRAMARLKVTSKYVKK"
     gene            12575..13717
                     /locus_tag="PLC71_00950"
     CDS             12575..13717
                     /locus_tag="PLC71_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM01245.1"
                     /translation="MNFELTPQQKAVQQSARQFAQTVLQETVLEDDAAGRFPEEAYKK
                     MGEMGFVGLPFPKEYGGQGGDYLSYVLALEEISKINSSVGIAYSVCTSLYSGALINDG
                     NEEQKKKYLPEVLSGKKMGSFCLTEPDAGSDAAGCKTTAIWNGKEYILNGLKCFITNG
                     PLADYYMVVALTNPELKTKGLTAFIVEREWEGVSIGKIEDKCGIRCAQVSEVIFDNVR
                     VPKENMLGEEGKGFAVAMKDLDGGRIGVAAQGLGIAKGAYDIAFNYLKEREQFGKPLY
                     KNQYLAFKMAELETEIEMAQYMLYKAATDKQEGRSYSVPAAKAKMVCTDAAMHVATEA
                     VQMLGGNGYMKEYHVERMLRDAKITQIYEGTNEIQKLVISGNMFRK"
     gene            13739..14920
                     /locus_tag="PLC71_00955"
     CDS             13739..14920
                     /locus_tag="PLC71_00955"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HRM01246.1"
                     /translation="MKKALEGIKVVDLTSALSGPFCTMMLADYGADVLKIEPLHGDQC
                     RTWGPIDEKSGESGFYCYVNRNKKGATLNLKSEKGKQMFYDLVKDADILVENYRGGVT
                     KKLGIDYETVKKINPSIIYASCSGFGQYGPLTHRACYDIVAQAMGGMVNLTGFKDTDP
                     VKVGPSVADHVSGIYLTVGVLAALHHRDMTGEGQQVDVSMFDTIFSLLENALVNYTMA
                     GEISQRNGNIDPSIAPFDIFPCKDGFTALGVGNDRLFDTFCHTIGHEELLGDPRYETN
                     DLRCKNYLPELQELIRGWCMEHTKKEIEYIMDEAGIPCGPVLDVKEAIEHPHTQAREM
                     MVHCEHPTAGDLYFQGCVTKMSETPGVVETPSPLLGEHNREIFGLTEEEEAQLKEEGV
                     I"
     gene            14949..15413
                     /locus_tag="PLC71_00960"
     CDS             14949..15413
                     /locus_tag="PLC71_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-alanyl-CoA:ammonia lyase"
                     /protein_id="HRM01247.1"
                     /translation="MRKVSYLSYNMTTADANNPDGIVPVGRQFDFIGDVETEEMILVD
                     GDESLCLGYEDVKIYQDVYVGDMMEYKATLTHIGNTSRDCRIEVFKLATPAYRAGKED
                     YKPGDMVWFDEPVLCTEGNVRLVVKKHLQRGEQPDGAVIDPWRHLDDFPEDE"
     gene            15447..15815
                     /locus_tag="PLC71_00965"
     CDS             15447..15815
                     /locus_tag="PLC71_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HRM01248.1"
                     /translation="MKDTITFRYRMSDRDVFYGGGVVNGARSITLMEDAAKRLMAKTF
                     GNTGRCVEVKKVRLYTPCFAGDYMEYIAGIRKQEGNKVLIEVRSFKIIEVPADPPYPS
                     SIDVLEDPPLSTVVQFWYEY"
     gene            complement(15946..16818)
                     /locus_tag="PLC71_00970"
     CDS             complement(15946..16818)
                     /locus_tag="PLC71_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009264566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM01249.1"
                     /translation="MSAKKARYCIGELSTLCNIPQKTLRYYDEIGLFTPDYRDDSTHY
                     RYYSKSQIVNLMIIKTLKQMGFPLKDIRQIISENDAQSLEANINSHLETMRDDIIKRI
                     DQYTECNYLLQKIQNGIDILEASSSLPSEDLAISIEHIPKISLMYDRSEMEGYDYSEM
                     SLDRWISILRKAEHSKHKIMGPVYVTFYEDNIMNKFLSANSMVEFAVHIEDTSSTDDI
                     RDFGDFDAVTMIHIGNYEDIPTSYIQMMKWIRQNGYTVTGPATEEFILSPLDVNDETR
                     RVTKIIIPVEKESK"
     gene            complement(17057..18313)
                     /locus_tag="PLC71_00975"
     CDS             complement(17057..18313)
                     /locus_tag="PLC71_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HRM01250.1"
                     /translation="MYECSLERMTDKIKTMSQFGDAGHGGITRYSLSPEALQARGEFV
                     RRMEAIGATIKFDDMANLYATLPGSEPDLPGIVMASHCDSVKNGGNYDGILGVMGAME
                     VLETVADQNIPHKHNLTAMIWTNEEGSLYPPAMMSSGVICYDYLPEDIRVNFKHEDML
                     KSTSVLDATKTFGAALDASGYKGDKANRLNNKDYKAMFELHIEQGPILEAAGNDIGVV
                     TCVLGMVNYTIKVYGQSDHAGTTPMKYRQDALYGASKVLQYLHDELDKLDPELVYTTG
                     EIFCHPNVHTVIPDYVEFSLDARHEKPEVIEQVVKVIENIPKEVVKCKTDYEIAWTRD
                     TVYYDKELVSYVQESVDELGYSNQKINSGAGHDAQFAAYMLPTTMVFVPSKDGHSHCE
                     PEYTSPEQCTQGASVLLNAVLKCDAK"
     gene            complement(18338..18775)
                     /locus_tag="PLC71_00980"
     CDS             complement(18338..18775)
                     /locus_tag="PLC71_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRM01251.1"
                     /translation="MNPYIQIIAAFFGSLGFALLFNLRGQWLFFTALGGGLSWCVYLI
                     SGSFISHYERQYFAAAFFLGIYSELMARKTKSPSTLYLVVGMIPLIPGAALYYMMRNA
                     IQTNFKMFAERGLEAVITGGAIASGIITAMVCWNVIKLNLLKK"
     gene            complement(18791..19567)
                     /locus_tag="PLC71_00985"
     CDS             complement(18791..19567)
                     /locus_tag="PLC71_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRM01252.1"
                     /translation="MKRKKLRLTPKQAEHMLFNVLEIGSRMLVSGAEVQRVENSIYRM
                     CTAYGSETTEVFCMTYMIVVTISSKQFGTITLSRRIHNLSLNLYELLYLNALSRRMCS
                     TLPDMKTVDQKIRQIKALPKYGPIPSMLIYALISSSFTIFFGGSLPDAAVSGMIGIGI
                     RCVELLLQPLPLNRFLLVVLLSTVSGCMAASCVYTNAMFSLDKISIGNIMILIPGLMF
                     TNCIREMISENMLSGLTRLLEALFISSSIAIGFAIAYYLL"
     assembly_gap    19585..19594
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19616..>20371)
                     /locus_tag="PLC71_00990"
     CDS             complement(19616..>20371)
                     /locus_tag="PLC71_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRM01253.1"
                     /translation="AWYHYMSRPEFVEAEADKRVVHWASHLNTPVYIVHMADKEGLEA
                     CIKAKSEGHPILIETCPQYLEFTCDVYKREDGRNFVCSPPMKGQESQDALWKAIKNGM
                     IDTVATDHCPFQSYEKDWGKDDFTKIPNGCPGVENLYPYMLDAANSGKITFERAVELC
                     STNVAKIFGCDNKGSISVGKDADIVIYDKDKDFTISVDNMHSDLDHTIWEGKQLHGYP
                     IRTYLRGNVVYDNGDFVGTPGLGEYVKRSPRKH"
BASE COUNT         6646 a         3574 c         4410 g         5731 t
ORIGIN      
        1 cacaatttca tcttctacaa gatcatcggc tatctttgct attgaatctc cttttcttgc
       61 tttcttttga accaacataa ttaattttag tgtctctccc tgtgtaattc ctttttcaat
      121 tcctcttcct tctacagcat ctaagattgt acacattgta atcgctccct tcttatcttt
      181 ctctaaattt aacttcaaaa atctttcatc ttcagcaaaa atccttataa acttcagcat
      241 ctcatctgca tgtttaattg gtttatgatt ttcaatggtt ttataacctt tccttcgctt
      301 cacaaaataa tctgcaataa ttccaaaatc actctgaaac atctttattt gctcctctgt
      361 cagatatgct atctcaaata catttaagtg ttttccagtt tattccacat attttattta
      421 tttttagtat actatagtta tttcatgttt tcaatccatc ttctctacac ctttcctcca
      481 caatcaacct atgttaaact aattatataa caattattca acgacatatt acataaacca
      541 aaaggaatcc taacacatga ctttagaaga agaatatcgt ctatcctgct atgaggaact
      601 gacgactctt ggaaatcaca atcatatata tttaatcaaa cacaagttgt ctggtgaaat
      661 tttcgttaag aaagttttat ccagattttc tttggatgtt tataaacagt tgagggactt
      721 acaaatctcc ggaattccaa aaattcatga cctgatcctt gaaaataatt ctttgattct
      781 gatcgaagat tatattcatg gacaaacttt ggaacagctg cttgaggaac agacaacact
      841 ggtctattcg gatgctcttg cgatcatgcg taagctttgt gatgtgttaa aatcacttca
      901 tactctgact cctccaatca ttcatcgtga tctgaaattg tcgaatatca ttttgaccag
      961 tgaaaatctt gtctatatcg tggatttcga tactgccaga tattatgaat ctgggcaaga
     1021 gcaggacacg gaattacttg gaacgaaaga atatgcccct ccagaacagt atggatttgg
     1081 gcaatctgat gcccgatctg atatttatgc gttgggaatt atttttaatc gcttattgac
     1141 aggggaatat ccgaaacatc aattggctga tgaccgatac cgttccatca tttcaaaatg
     1201 tatccgctgg aatcctgaag aacgtttcca gacggttgag gaacttaaga cagctttaca
     1261 cgataatatt tcctctgata ttggaaaacc tggatttacg ctttcttcga tacattctga
     1321 tattccagga tttcgtactg gaaagctgtg gaaaatgatc ttggcattct tcgggtatct
     1381 ctcttttctt tactgtagta tgacttcaga tttcacaaat gcaaaaaccg gacttcctct
     1441 aactgggcta caactatggc atgaacgttt ttttgtattt ctgatgctgc tcagccttat
     1501 ttttataatt ttaactacaa aaatatccga accagatctt ttctgggtca ttctctccct
     1561 tttgtacttc gaattgttct acagtgtctg atttctttgg ggattttggt cattcttatg
     1621 atctttatgc tgctgatcga agaaatcata tggtaggttc tttatcgctg gcagtaaaaa
     1681 tttacgctgt cagcgaaaca tttctatatg aatatttgct actcttatta ttgttcatat
     1741 agaagggagg aattaaatat gaaaacttgg aaattagttt ctggaatcat ctcaattgta
     1801 ctttttgctt ttgttatgtt tcagtcttgt gctgctggaa tttcaaattc tttagcagaa
     1861 aatggggaaa gtggcggttc tgccggattg atcgttgcga tcattttatt ggctggtgga
     1921 attgtttcta ttgctacaag aaatggttca aagggcggta atatcgcact gatcgtttta
     1981 tttgcacttg gtgcactttg cggatttact atggcaggaa gttatgcaga tttaaatgta
     2041 tgggctgcct ggtgtctgat ctgtgcggta tttgcagcag tttccttaaa gaaggggcaa
     2101 tgatcgttta ttataagcac aaagagagaa aggatattta catatgcagg atcaattaac
     2161 tgatcattta gatcaacaat taaaatctac ttctttggag aatttttcga attatcttaa
     2221 gaatcttcag cagttaccaa gcctgcagga ttttttctct gtttatattg caaagcatga
     2281 cctgaaaatt tcgcagatca tcaaaaacag tgggatatca caaagctatg cccatgaaat
     2341 cttaaatgga acgaaacctc atccttccag agattatctg ctggctttgt gtcttggggc
     2401 tcatatggat ctaaaaacca cgcaacatgc tctgcgaatc gcacaactcg gtgaacttta
     2461 tgcaaaagtt ccaagagatg cagcgatcat gatgcatatc aataatgaaa aatggaatct
     2521 cattgatatt aatatctttc tggaagaaca tggtcttaat gtgatttcac tttcaaagaa
     2581 aatttcataa ataatagcaa aaagatcagc cgtaaaactt tctacagctg atctttttta
     2641 tattttcttc gttatatgat gtaagtgtca aaagtataaa ttgcgtttgt gcttgcacaa
     2701 ataagcaatt tatacttatt gacactctta tcactatata tctgatagat agctttcaat
     2761 gccatctaag atacattgaa cagctttttt ctgataccca tcatcctgaa gagctttcaa
     2821 ttcctcagga ttagataaaa aaccacactc cacgatcacg atcggataag gattatcccg
     2881 aaacagataa tactcagcat ttgcctttgc ctccctgtga ttgttcggat caagcccctt
     2941 gatcaaagca gactggatat gatccgctaa ctgctttgat ttctcagacc cttgcggata
     3001 gaaaacctga gctccatgga catcactgct tggataacta ttctgatgaa tgctgatcac
     3061 tgccatagga tcattctcaa aaatgagctg ctttcgattg gatagatcac ttcgtttctc
     3121 attttctttc gaatccgcaa gattatagtc cccatttctt gtcatatata ctttataatt
     3181 tttcttttcc agaagttttt gaagctttaa tgcaagagaa agattgatat ccttttctaa
     3241 aatattcgaa tttccaacct tcccaggatc agaaccgcca tgtccggcat cgatacagat
     3301 cacattttga ttcttgacag tctgtgcaac aaagataggg aatgtaatag aagaaacaga
     3361 taataaaacg acgatcagaa gaacacccca gaagatgaaa cgatgttgtt gattcatagg
     3421 aagaacatcc tttttctttt tattatatga tgtaagtgtc aaaagtataa attgcgtttg
     3481 tgcttgcaca aggaagcaat ttatatttat tgacacgctg aacaacgaga acggaaaaaa
     3541 tggtgtgaat acgccatttt atgggagttc gaggtgttcg aggattgcgg aattgcggag
     3601 caatggaaca atccgggtac atcagagagt ggcaaaattt attttgacac tctcatcatt
     3661 atatgatgta agtatcaaaa gtataaattg cgaagtctca atggtgcaat actttatttt
     3721 ttccatattt aatgttataa taaatccaaa tgactacatt taatcattga agaaagatta
     3781 aaagtcagga gagtttaata tggaaacaag acttgtaaaa attaataata tggaagattg
     3841 caaaaaggac atggaagaag caggagagct gatcaaagca ggcgaacttg ttgcatttcc
     3901 aacagagaca gtctatggtc ttggagggaa tggattactt cctgatgcgg caaagaagat
     3961 ctatgcagcg aagggaagac cttctgataa tccattgatc cttcacgtat gtgatatgca
     4021 gatggtagaa tctctggtca aagagattcc agacaatgca agacgtgcga tggaacattt
     4081 ctggccggga cctatgacgg tgatcttgca gaaagcggat caggtaccag attgtgtgac
     4141 aggcggactt gatacagtag cgatccgtat gccggatcat ccagttgcat tagagctgat
     4201 cagaagatca ggagtgccga tcgcagcacc aagtgcgaat acatctgggc gtccaagtcc
     4261 gacaaaagca gaacatgtca tggaagatct tcatggaaag atcccgatga tcttagatgg
     4321 gggagctgtt atggtaggtg tagaatcaac gatcattgat tttacagaaa cagttccagt
     4381 gatcctaaga ccaggatgga tcacaaagga gtccatggaa gaatttcttg ggtgtgaagt
     4441 attgatgaat acatctttaa aagcaagcga tcacggaatt ccaagagcac ctggaatgaa
     4501 gtacaaacac tatgcaccaa aggcggagat ggtgcttgta ttaggcgatg atcaggagaa
     4561 agtcgttgat catatcaatc agcttgcgaa agaacaaaag gaacagggta agaagatagg
     4621 aatcattgca tcggatgaga caaaagacct ctatcaggca gatgaagttg tttctgtagg
     4681 acagagagca catctcgaga cagtaacagc acatttatat gatgtattaa gagaatttga
     4741 ccataaggat gttgatatga tctattccga aggatttgaa ggtgaaccct taagtgaggc
     4801 gatcatgaac cgtatggtaa aagcagcagg acatgaaatc ctccgtattt aaaaaggaga
     4861 atattttgaa gaagataata atggtgagta cgaataatac gtgccgaagc tttattgcag
     4921 agtcgatcct tcgccagtat cttttggatg cagatcagaa gaatgttgaa gtgatctcaa
     4981 gagggctggt cgtattattt ccagaacctg tccatgtcaa agcagcagac atggtaataa
     5041 agacgtgcgg gattgaaagt cttgattttc ggtcggcgca gcttacgcag gaagaagtag
     5101 aagaaagcga tctgatctta accatgacag aagagcagaa agataagatc ttagaagaat
     5161 atcatggata caaagagatc gcgacattga acgaatatgc cagagtggaa ggtgcgatca
     5221 ttgacccata cggcatggaa gatgacgact acgagcgctg ttttgaacag atcgcccgtt
     5281 tagttaagaa aatatggcaa gaaagactag gaggaaacaa aatgataggt attggtagtg
     5341 atcacggtgg atatgcatta aaacaggcag ttatcaaaca tctggaagaa aaaggatatg
     5401 cagtgaaaga ttatggatgc tattcagaag aatcctgtga ttatccagtt tatgcaaaag
     5461 cagtagcgaa tgcagtcaaa gacggagaag taaaacaggg aatcttaatc tgtggaacag
     5521 gaatcggaat ctctattaca gcgaataaga ttaagggaat ccgtgcagca ctttgcagtg
     5581 acacattcag cgcacacgca acaagagaac ataatgatgc aaacattctt gcaatgggag
     5641 cacgtgttgt tggagaaggt ttagcacttg atatcgttga tacattcctt gagacagaat
     5701 tctcaaatga tgagagacac attcgtcgta tcaatatgat cgaagactag aagagaaaat
     5761 caaagttata taaaaataag atataagcca tatgaataaa gaagatatgc agacagtttt
     5821 ttagaagtga tcaacagttt gcatgtcttt ttttgtatac aaaatcactt tgaagatgat
     5881 aaaaaacagt taagattcag ttaagaagca gaaaaaaatt atcagatctt aacacaaaaa
     5941 aactgaccaa tgttcatttg tatacactga aaacccttat gttttggtgc tttttactaa
     6001 atttagaagt ggttatttgt aagtattaaa caaaaaacaa acacaatctt aacggaaaaa
     6061 gaattgacaa ataaaaattt gaggcataaa atagctttca gaacaagggt gtttacgatg
     6121 tattgtgtaa gcactctttt acttttgcag gaattaaacc cttggatctc tgcaaaaaat
     6181 taaaattaaa gaaggtgagg agaaaatggg ttcattggga gattatctga tggaagagtt
     6241 gtcacttaag acaagcttta cgatccccat cggcggaggt attccagtat cacagtcagc
     6301 cgtaacaaca tgggtgatca tggcaatcgt agtgttgctc tgcatcatat tcgtacggaa
     6361 cctgaaagtc ataccagaag gaccgcaagt ttatgtggaa gccttagttg gtttcatcta
     6421 taacttcatc ggtggtctgg tcggcgaaaa agggaaacgt tacataccat ttcttggaac
     6481 aattctgatt tatcttggct gtgcaaatat ttcgggattt ttcagagcga aaccgccaac
     6541 gaaagatctg aatgttaccg caggattagc gatcatcagt ttgtttttga tcatatattc
     6601 cggaatccac gagaaaggat tgaagggata tatacacagt tatgtggaac ctatgccaat
     6661 gatggcggcc attaacatcc ttgaattatt tacaagaccg ttatcactgt gtatgcgact
     6721 ttttggaaat gtcttaggag cattcgtttt gatggaattg atcacacata tcgcaccagc
     6781 aatcgtgcca ttaccattta gttgttactt cgactttttc gatgggttgt tgcaggcata
     6841 tgtatttgta ttgttaacat cactgtttat cagtgaatca attgagtaag atgtatcatg
     6901 acagatacac aaatttaatt tttatttaaa aggagattag aactatggga ttagcagcta
     6961 ttggagcagg aattgcagta ttaacaggtt taggagcagg tattggtatc ggtgttgcaa
     7021 caaatggagc acttggagca atcgcaagac agccagaaga agctggaaac atcaataaaa
     7081 cattattact tggttgtgca ttagccgaag ctacatcaat ttatggatta gttgttgcga
     7141 ttctgttaat cgtaatgaaa tagaagtagg aggcggaaga tatgtttctg aaattagact
     7201 ggggcattat ctggccgatc gtcaacattg tcgtatttta tctcttgctt agaaaattct
     7261 tatttggtcc agtcagtgag gtcatggaga aaagaaaaaa aatgatctca agtgacttag
     7321 atgatgcagc acagacgaaa gctgaagcag aagagatcaa gcaggaatat gagaaaaacc
     7381 ttgcacaggc aaaggatgag gcaggacaga tcgtttccga cgcaagagca agagccaaga
     7441 atgaatacca gaataagatg gatcagacaa aagaagagat tgctcttatg agagaaaatg
     7501 cccgtaaaga cattgaagct gagaaacaaa agacgatcgc aggtcttcag acagagatcg
     7561 caggtatcgc acttatggca gcttcgaaag ttgtggaaaa agaagcaaac gacaaaggca
     7621 acgagaagct tttggatgat ttcctgaaag aggcaggtgt ctagcatatg aagagaattt
     7681 ccatgacgta tggacaggta ttgtttgaac ttggaatcaa aaaagaaagt ttacaaaaag
     7741 cacaggacat gcttcatgaa aatgaagagc ttttaagtgc attagagaat ccaacgatca
     7801 caaagaaaga aaaagaaaat gtggtggaaa agttgttttc tgatgatata aaaagtttct
     7861 taaaagttgt atgtgataat gatgatatcg cttgttttga cgaggcagtt gagtattatg
     7921 atgaactaaa acgcaagaca gataagatca ttaaggcaga gtttgactat gtgacaatgc
     7981 caaaagacga acagcttgag cgaatcaaac agtatcttat gaaacaatat caggcggaca
     8041 aggtagagct tactttaaaa gaagagaaag acttgatcgg tggttttgta ctgaaagttg
     8101 gagaccacgt ttatgataac agcctgagtg gaaaaatgag aaaattacag cagaaactta
     8161 catggaggtg aggatcaggt gagcagtatc aatccagaaa cagtcgtttc tgtattaaaa
     8221 gatcaaattg aaaactatga ccagaaaact gaggtcaaag aagaaggaag aatcattcag
     8281 atcgcagacg gtattgcaac agtctatgga atggaccagg caatgtatgg agaacttgtt
     8341 atttttgaaa caggtgtcca cggacttgta ctgaatgttg aaagaaactg tatcggttgt
     8401 gttttacttg gttccgatca tggattaatg gaaggatcta aagtagcaag aaccggtgta
     8461 caggcagata tcccggttgg agatgctatg atcggacgtg tcgttaatgc aatcggagaa
     8521 ccaatcgatg gaaaaggtga gattaaaact gacgagagaa gaccaatcga acatgaggca
     8581 gcaggagtta tcgacagaaa atctgttgac cagccattac agacaggtat cttagcgatc
     8641 gactctatgt tcccgatcgg acgtggacag cgtgagctga tcatcggtga ccgtcagaca
     8701 ggtaaaacat cgatcgcagt tgatacgatc ttaaatcaaa aaggacagga tgttatctgt
     8761 atttatgtag cgatcggaca gaaagcatca acagtatcac agatcgtaac aacattaagt
     8821 aaacatggtg ctatggatta ctctatcgta gtatccgcat ctgcaagtga ccttgcatct
     8881 ttacagtaca ttgcaccata tgcaggaaca gcgatcgcag agcactttat gcatcaggga
     8941 aaagacgttt tgatcgtata tgatgatctg tctaagcacg ccgttgctta tcgtgcaatt
     9001 tccttactgc tggatcgtcc acctggacgt gaagcttacc ctggagatgt attctactta
     9061 cattcaagat tactagaacg ttcttgccga ttaagtgatg aacttggtgg tggatcaatc
     9121 acagcattac cgatcatcga aacacaggca ggagacgtat ccgcgtacat tcctacaaac
     9181 gtaatctcta tcacagacgg acagatcttc cttgaaagtg agttattctt ctccggtgta
     9241 agaccagcgg tcaatgttgg actttctgta tcccgtgtag gtggagcagc acagacaaag
     9301 atcatgaaga aagtagccgg aaaccttcgt attgacttag ctcagtatcg agaacttgag
     9361 gtatttacac agtttagttc agaccttgac gatggaacaa aagcaacatt aaattatgga
     9421 tctcatctga tcgaactgtt aaaacagcca ttgtaccatc caatggcatt acataaacag
     9481 attttacttc tgtttgcagc aggtcataag atgttaaatg atgttccagt caaagaatta
     9541 aaggcagaga cagaagaaat gacagcattc tttgaacaga aatatccaga gacagtgaag
     9601 aagatcgacg aaggcaaagt cttaacagat gacattgcaa aagagatcca agaggcgata
     9661 gaagcctatt taaagtaggt gatcactatg gcaaatatca gagaaattaa aacaaggatg
     9721 aacagtatcg aagatacatt aaagatcaca aatgcgatgt atctgatctc atcttccaaa
     9781 ttaaagaaag caagaaagca gttagatgcc acaactccat actttgaaat gcttcaggca
     9841 tctctgcttg atatcatggc acatacgcca gatcttcagc acattttctt aaacaaaaga
     9901 aagaaagaac atccaaaaaa aggatatctt gtgatgactg cagaccgtgg acttgccggg
     9961 gcttataatc ataacattac aaaaatggca gaagagaata ttaatgaaga tgataaactg
    10021 ttcgtgatcg gatatatggg acgtaattat ttcggacgaa gaaatgcagc ccagatggac
    10081 gaggatttct tctatacaac acaagatcct acgatctaca gagcaagaag aattgcagaa
    10141 aaaatgatcg aggaatatgt atcaggagaa cttgacgaag tctatattat ttatactaag
    10201 atgatcacat ccattacctc agaaccaaag atgatccgtg tgttaccatt aaacccagat
    10261 cagtatgagt ttgcaggaaa aggaaagaga tcacatatga cagaatttca gccatccgta
    10321 aaggcggtgc ttgaccgcct ggcaccaaac tatatcaaag gacttgtcta tggtgcgatg
    10381 gtagaatcat tttccagtga attaaacgcc agaatgatgg cgatggaagg tgcaagcaag
    10441 agtgcgagag aaatgataag atcactggga cttatgtata accgtgctcg tcaggcagcg
    10501 atcactcagg agatcactga ggtgtgtgcc ggagctaaat cattaaagaa agagtaacag
    10561 tgaaggaagg gaaataaaat ggaaaaagga aaaatcgtac agattcttgg tccagtaatc
    10621 gacgtagaat ttgaaactga acagctccct ttgattcatg acgccttgac cgttgttaat
    10681 aaaggaaaag aagttgttat ggaagtggaa cagcatatgg gaaatcgtac tgtacgctgt
    10741 atcctgttag cagccagtga aggacttcac aaaggaatgg aagtcacagc aactggtgga
    10801 ccaatcaaag ttccagttgg aaaacagaca ttaggacgta tgtttaacgt acttggagaa
    10861 gcaattgata aaggagaacc tgtagaatca gaagaaaaat gggcgatcca tcgtgaacca
    10921 cctaaatttg aagaccagag ccctgttgta gaaatgtttg agacaggtat taaagtcatc
    10981 gaccttttag ctccatatgc aaaaggtggt aagatcggtc tgtttggtgg tgctggagtt
    11041 ggtaagacag tcttaattca ggaactgatt cataatatcg caacagaaca tggtggatat
    11101 tccatcttca caggtgtagg agaacgttca agagaaggaa atgacctctg gaatgaaatg
    11161 acagaatccg gagttatcaa aaatacagcc ttagtctttg gacagatgaa tgagccacct
    11221 ggatctcgta tgagagttgc ccagacagga cttacaatgg cagaatattt ccgtgatcag
    11281 gaaaaacaga acgtgttatt attcatcgat aacattttcc gttatgtaca ggcaggatca
    11341 gaggtatcag cgctgttagg acgtatgcca tcagccgtag gttatcagcc aacactggca
    11401 aatgaagttg gtgctctaca ggaacgtatc acatctacaa agaacgggtc tatcacatct
    11461 gtacaggcag tttatgtgcc agccgatgac ttaactgacc cagcaccagc aacaacattt
    11521 gcccatctgg atgcaacaac tgtattatca agagcgattt ctgaacaggg tatctatcca
    11581 gccgtagacc cactggaatc aagctctcgt atcttagagc cagacatcgt tggagaagaa
    11641 cactatgaag tagcaagacg tgtagaagaa gcacttcaga gatacaaaga attacaggat
    11701 atcattgcaa tccttggtat ggaagaatta gacgaagaag ataaaatcac agtataccgt
    11761 gcaagaaaga ttcagaaatt cttatctcag ccatttaacg tagcagaaac atttacagga
    11821 atccctggta aatacgttcc tgtcaaagaa acgatccgag gattcaaagc gatcttagat
    11881 ggagaaatgg atgaatatcc agaacaggcc ttcttgatgg tcggaacaat tgatgatgta
    11941 aaggaaaagg caaaagaggt gatgtaaatg gcagaaacct tttattttga gatcattgcc
    12001 agtgacaaaa agttctacag cggagcttgt gaacacgtca ttttcccagc agtggacgga
    12061 ctttacggtg tattagcaaa ccatgaagat acagtaacag ccgttgttgc aggagagctt
    12121 cgttttaaag ttgatgggga atggaaagtt tgtattgttg gtgaaggatt cgcagatgtc
    12181 accagagatt tcgtagtcat cgtggtagat acggtggaac gcccagaaga catcgacatc
    12241 atccgtgcaa gagaggccaa aacaagagca gaagaacgac tcaaacagaa acaaagccgt
    12301 ttgcagtact atcatacaca ggcagcattg tcacgtgcta tggcacgact gaaagttaca
    12361 tcaaaatatg tgaaaaaata gagtctgaga agacaaatat agctaaatga gaaaaaagat
    12421 caactatcca gtaagtggaa agttggtctt ttttttgtac aatctgcata aaaaatatca
    12481 agaatactgc ttgactctgc actgaaagga tagactacac tacaaatata cgaggtagaa
    12541 aataaaatgt gcaatatgca gaaaggatga aactatgaat tttgaattaa ctccacagca
    12601 gaaggcagtt cagcagagtg caagacagtt tgctcagact gtattacagg aaacagttct
    12661 tgaagatgat gcagcaggac gtttcccaga agaagcatat aagaaaatgg gagaaatggg
    12721 atttgtcggg cttccatttc caaaagaata tggtggtcag ggaggagatt atttatccta
    12781 tgtattagca ttagaagaaa tctccaagat caattcatct gtaggaattg cttattctgt
    12841 atgtacttca ttatactctg gtgctctgat caatgatgga aatgaagaac agaagaaaaa
    12901 atatctgcca gaagttctgt ctggaaagaa aatgggatca ttttgtttaa cagaaccaga
    12961 tgcaggttct gatgcagcag gatgtaaaac aacagcgatc tggaatggaa aagaatatat
    13021 cctgaatgga ttaaaatgct tcattacaaa tggaccatta gcagattact atatggtagt
    13081 tgctcttacg aatccagaat taaagacaaa aggattaaca gcattcatcg tagaacgtga
    13141 atgggagggt gtatccattg gtaagatcga agataagtgc ggaatcagat gtgcacaggt
    13201 atctgaggtc atctttgata atgtaagagt tccaaaagag aacatgctcg gagaagaagg
    13261 aaaaggattt gctgttgcaa tgaaagacct ggatggagga cgtattggcg ttgcagccca
    13321 gggacttgga attgccaaag gagcttacga catcgcattt aattatttaa aagagagaga
    13381 acagttcgga aaaccattat acaagaatca gtatttagca tttaagatgg ctgagttaga
    13441 gacagagatc gagatggctc agtatatgtt atacaaagca gcaacagata agcaggaagg
    13501 cagaagctac agtgtaccag cggctaaagc gaagatggtt tgtacagatg cagcaatgca
    13561 tgtagccaca gaagctgtac agatgctggg tggaaatgga tatatgaaag aatatcatgt
    13621 agaaagaatg ctgcgtgatg ctaagatcac acagatctac gaaggaacaa atgaaattca
    13681 gaaacttgtc atcagcggaa atatgtttcg taaataagaa caaataggag gtgtcgaaat
    13741 gaaaaaagca ttggaaggaa ttaaggtagt tgatcttact tcagctttaa gtggaccgtt
    13801 ttgcacgatg atgttagctg attatggagc ggacgtatta aaaatcgagc ctctacacgg
    13861 agatcagtgc agaacatggg gtccaattga tgaaaagagc ggagagagcg gattttactg
    13921 ttatgtaaac agaaataaaa agggtgcaac attaaacctg aaatctgaaa aaggaaagca
    13981 gatgttttat gatctggtaa aggatgcaga tattcttgtt gagaattatc gtggcggggt
    14041 tacaaagaag ctggggattg attatgagac ggtaaagaag atcaatccat ccattatcta
    14101 tgcatcctgt tccggatttg gacagtatgg accattaaca cacagagcct gctatgatat
    14161 cgtggcgcag gcaatgggcg gaatggtgaa tctgactggt tttaaagata cagatccagt
    14221 caaagttggt ccatctgtcg cagaccatgt atcagggatt tatttaacag ttggtgtatt
    14281 ggcagccctt catcatcgtg atatgaccgg agaagggcag caggttgatg tatctatgtt
    14341 tgatacaata ttcagtcttc ttgaaaatgc cttagtgaat tatacaatgg caggggagat
    14401 atcgcagaga aatggaaaca tagacccatc cattgctcca tttgatattt tcccttgtaa
    14461 ggacggattt acagcactcg gggtaggaaa tgacagatta tttgatacat tctgtcatac
    14521 gatcggacat gaagagctgc ttggtgatcc aagatacgaa accaatgatc taaggtgtaa
    14581 gaattacctg ccggaactgc aggaactgat ccgtggatgg tgtatggaac atacaaagaa
    14641 agagattgaa tatatcatgg atgaagcagg aattccatgt ggaccggtat tagatgtaaa
    14701 agaagcgatc gaacatccac atacacaggc aagagagatg atggttcact gcgaacatcc
    14761 tacggcagga gatctttatt tccagggatg tgttacgaag atgtcagaaa ctccaggagt
    14821 tgtagaaacg ccttccccac ttctaggaga gcataacaga gagatctttg gattaaccga
    14881 agaagaagaa gcacagttaa aagaagaagg cgtaatttaa ttacttttat ttcataagga
    14941 ggagcaacat gagaaaagta tcttatttaa gttacaatat gacgacagcg gatgcaaata
    15001 atccagatgg aatcgtgcca gttggacgtc agtttgactt tatcggagat gttgaaacag
    15061 aagaaatgat cttggtagat ggagatgaat ctctctgtct gggatatgaa gatgtaaaga
    15121 tctatcagga tgtgtatgta ggagatatga tggagtacaa agctacttta acacatatcg
    15181 gaaatacatc aagagactgc aggattgaag tatttaagct ggctacacca gcatacagag
    15241 ctgggaaaga agactacaaa ccaggagata tggtatggtt tgatgagcct gtattatgta
    15301 cagaaggtaa tgtaagactg gttgttaaga aacacttaca acgtggagaa cagccagatg
    15361 gagcagttat tgatccatgg cgtcatctgg atgattttcc agaagatgaa taaagtacat
    15421 aagacagatt gaaaggagaa agaatgatga aagatacaat cacttttcgt tatagaatgt
    15481 cagaccgtga cgtattttac ggtggtggag ttgtaaatgg tgccagaagt atcacactga
    15541 tggaagatgc agcaaaacgt ttgatggcaa aaacatttgg aaatacagga agatgtgtag
    15601 aagttaaaaa agttcgtctt tacacaccat gttttgccgg agattatatg gaatatatcg
    15661 ctggaatcag aaaacaggaa ggaaataaag tactgatcga agtccgttct tttaagataa
    15721 ttgaagtacc agcagatcct ccatatccaa gttctattga tgttctggaa gatccaccat
    15781 tatcaacagt tgtacagttc tggtatgaat attagatctt ataaagctca ttcatagtat
    15841 taataaataa taaacctggc aaaaaggctg taaattcttt ggggtaagca aagaaattta
    15901 cagccttttt ggtgtgcatt cttattttgt ataacaaata ttaatttatt tactctcttt
    15961 ctcgacaggg ataatgatct tggtcaccct gcgagtttca tcgttgacat ccagagggga
    16021 aaggatgaat tcttctgttg ctggtcctgt aacagtgtag ccattctggc ggatccattt
    16081 catcatctgg atataagaag tcgggatatc ctcatagtta ccgatatgga tcatggttac
    16141 ggcatcaaaa tccccgaaat cacgaatatc atcggtagaa gaagtatcct cgatatggac
    16201 tgcaaattcg accatggagt ttgctgagag gaacttgttc atgatattat cttcataaaa
    16261 tgttacataa acaggtccca tgatcttatg tttggaatgc tctgctttcc gaagaatgga
    16321 gatccatcgg tctaaggaca tctcagagta atcatatcct tccatttcag aacggtcata
    16381 cattaaactg atcttaggaa tatgttcaat agagattgca aggtcttcgg aaggaagaga
    16441 agaagatgct tctagaatat caattccatt ttgaatcttt tggagcagat agttgcattc
    16501 ggtgtactga tcgatccgtt ttatgatatc atctcgcatc gtctcaagat gtgaattgat
    16561 attcgcctca agggactgag catcgttctc agatatgatc tgccggatat cttttaatgg
    16621 aaagcccatt tgttttaaag ttttaatgat catcagattg acgatctgtg attttgaata
    16681 ataacggtag tgcgtactgt cgtctcgata gtctggtgtg aataatccga tctcatcgta
    16741 atagcgtaaa gtcttctggg gtatattaca gagggtcgat aattcgccga tacagtagcg
    16801 cgctttttta gctgacataa attacctccg attcataagc aataagaatg cgaatcataa
    16861 aataaatatt acctatctgt tcattatatc ataaaagatg gataaatcat agatgatttt
    16921 attcaaaatg cataaaaata aaaagataat tcttatagaa attgtataaa atacacagaa
    16981 gaataagaga tatatcctag aggatatatc tcttattctt gacttatatg aggaagatat
    17041 tgttagcgaa atgtatttat tttgcgtcac atttcaatac ggcatttaat aatacggaag
    17101 caccctgtgt gcactgttct ggagatgtat attcaggttc acagtgagaa tgtccgtctt
    17161 tagatggaac gaataccatt gttgtaggaa gcatataagc tgcgaactga gcatcatgtc
    17221 cagcaccgga gttgatcttc tggttagaat atccaagttc atcaacagat tcctgaacgt
    17281 aagaaacaag ttctttgtca tagtaaacag tatcacgtgt ccatgcaatt tcataatcag
    17341 tcttgcattt tacgacttct ttaggaatat tttcaattac tttgacaacc tgttcgatta
    17401 cttctggttt ttcatgacgt gcatctaatg agaattctac ataatcagga attactgtat
    17461 gtacgtttgg atgacagaag atctcacctg ttgtataaac aagttctgga tctaatttgt
    17521 ctaattcatc atgcaggtac tgaagaactt tagaagctcc atataaagca tcctgacgat
    17581 acttcatagg agttgttcct gcatggtcag actgtccata aactttgatc gtgtagttaa
    17641 ccattccaag aacgcaagta acaacaccaa tgtcatttcc agcggcttca aggataggtc
    17701 cctgttcgat atgtaattcg aacattgctt tgtaatcttt gttatttaaa cggtttgctt
    17761 tgtcaccttt gtatccagat gcatcaaggg cagcaccgaa tgtctttgta gcatcaagaa
    17821 cagaagtaga ttttaacata tcttcatgtt tgaagtttac acggatatct tctggaagat
    17881 agtcatagca gataacaccg gaagacatca ttgctggtgg atataaggat ccttcttcat
    17941 ttgtccagat catagctgtt aaattatgtt tatgagggat gttctggtca gcaactgttt
    18001 caagaacttc catagctccc ataacaccta agattccgtc ataatttcca ccgttcttta
    18061 cggaatcaca gtgagaagcc attacgattc caggaagatc tggttcactt ccaggaagtg
    18121 ttgcatataa gttcgccata tcatcaaatt taattgtagc tccgatcgct tccatgcggc
    18181 gtacaaactc acctcttgcc tgcagtgctt ctggagaaag ggaataacga gtgattccgc
    18241 cgtgtccggc atctccgaac tgactcattg ttttgatctt gtctgtcatt cgttctaaac
    18301 tgcattcgta cataatagtt acctcctaaa gattgattta cttttttaat aaatttaatt
    18361 tgatcacgtt ccaacatacc atggcggtaa tgataccaga tgcgatcgct ccacctgtaa
    18421 taactgcttc taagccacgt tcggcaaaca ttttaaaatt cgtctgaatg gcatttcgca
    18481 tcatataata aagtgcggct cctggaatca atgggatcat gcctactacc agataaagag
    18541 tggaaggtga tttcgtttta cgtgccataa gttcggaata gattccgaga aaaaaagctg
    18601 ctgcaaaata ttgccgttca tagtgagata taaaggatcc gctgatcaga tatacacacc
    18661 aggagaggcc tccgcctaag gctgtgaaga acagccattg tccgcgcaga ttaaacaaaa
    18721 gcgcaaaccc aagagatcca aaaaatgcag caatgatctg tatatatgga ttcatggtac
    18781 aagcctcctt ttacagcaga tagtaagcaa tggcaaaacc aatggcgatg gaagaagaaa
    18841 taaatagtgc ttccaaaagc cttgttaagc cggataacat gttttctgag atcatttccc
    18901 tgatacaatt ggtaaacatc agtccaggga tcaagatcat gatatttcct atgcttatct
    18961 tatccagaga aaacatagcg tttgtataaa cacaggatgc ggccatacag cccgatactg
    19021 ttgacaataa tacaaccaga agaaaacggt ttagaggcag tggttgaagg agaagttcga
    19081 cacatcgtat accgattccg atcataccgg aaacggcggc atcaggaagg ctgcctccaa
    19141 aaaatattgt gaaagaagag gagatcaaag catatatgag catggagggt attggaccat
    19201 actttggcag cgctttgatc tgcctgattt tttgatcgac cgttttcata tccggaagag
    19261 tggaacacat ccttctggat aaagcattta aatagagtaa ttcatataga ttcagggaaa
    19321 ggttatgaat ccgtctcgac aaggtgatcg tgccaaattg cttggaggaa attgtaacaa
    19381 cgatcatgta ggtcatgcag aagacttcgg tcgtttccga tccatatgca gtacacatac
    19441 gatagatcga attttcgacg cgttggacct cagctccgga gacgagcatt cggctgccaa
    19501 tctccagaac attaaagagc atgtgttcag cttgtttcgg cgtaagtcgt aatttctttc
    19561 tcttcataag aatcctctga ttttnnnnnn nnnntaagtg tttgtattat agggtttagt
    19621 gttttcttgg gcttcgtttt acatattcac cgagtccagg tgttccgaca aagtctccat
    19681 tgtcatatac aacatttcca cgaaggtaag tacggattgg ataaccgtgt aactgttttc
    19741 cttcccagat cgtatgatcc agatcagagt gcatattatc aacgctgatc gtgaagtctt
    19801 tgtctttgtc gtagataacg atgtcagcat ctttacctac actgatagat cctttgttgt
    19861 cgcatccaaa gatcttggct acgtttgtag agcataattc aacagctctc tcaaaagtga
    19921 tctttccgct atttgcggca tccagcatat atggatataa gttttctaca ccagggcacc
    19981 cgttagggat ctttgtaaag tcatctttac cccagtcttt ttcataactc tggaatgggc
    20041 agtggtcagt agcaactgta tcgatcattc cattcttaat tgctttccat aaagcatcct
    20101 gactttcctg tcctttcata ggaggagagc agacgaagtt tcttccatct tcgcgtttgt
    20161 atacgtcgca tgtaaattcc agatactgtg gacaagtctc gatcagaatc ggatgtcctt
    20221 cggattttgc cttaatacat gcttccagtc cttctttgtc tgccatatgt acgatatata
    20281 ctggtgtatt caggtgggaa gcccagtgaa caactctctt gtctgcttct gcctctacaa
    20341 attccggccg gctcatgtaa tgataccagg c
//
LOCUS       DAOMUE010000008        20044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_451_length_20044_cov_2.711241, whole genome shotgun sequence.
ACCESSION   DAOMUE010000008 DAOMUE010000000
VERSION     DAOMUE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 20044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20044
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_451_length_20044_cov_2.711241"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..1071)
                     /locus_tag="PLC71_00995"
     CDS             complement(220..1071)
                     /locus_tag="PLC71_00995"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM01254.1"
                     /translation="MKKIWKIMILCMILCFFCAGCSNDDEMISEDHPKPTTEKRTEII
                     KQKTTQKKNEVTVDQEQSKQQEDRIKIAIDAGHQKKQMSEKEAIGPGSDKTKPMVSSG
                     TEGIVTKRTEYQVNLEVSLKLKSALIARGYDVYMIRETNDVSLSNKKRALMANESGSD
                     ILLRIHCNSADSQSANGALTMSPTLSNPYCRSIAADSQELSECVVSTLCRRTGAVNRG
                     VTQTDEMTGINWSKIPVTIVEMGFMSNPEEDQKLSDNHYQSMLAEGIADGVDRYYERR
                     GEKNEEK"
     gene            complement(1109..1654)
                     /locus_tag="PLC71_01000"
     CDS             complement(1109..1654)
                     /locus_tag="PLC71_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HRM01255.1"
                     /translation="MIDEILKYNKEFVEEKKYEQYKTSKYPDKKIAIITCMDTRLTEL
                     LPAALGIRNGDVKIIKNAGGVVSHAFGSVVRSVLVGIFELGIEEVMVIGHTDCGVQHI
                     NSDMMIKHMMDRGVDEEKIELIRHCGIDFDTWLEGFDCVESSVKDSVEMLRKHPLIPK
                     DVKISGYVMDSVTGELHVVEP"
     gene            complement(1667..2149)
                     /gene="ybaK"
                     /locus_tag="PLC71_01005"
     CDS             complement(1667..2149)
                     /gene="ybaK"
                     /locus_tag="PLC71_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HRM01256.1"
                     /translation="MAKKEPKTNAMRMLERAKIPFEVHRYECKEFIDGITIADMLNEP
                     YERVFKTLVTEGKGGGYFVFVIPIDQELDLKKAAKAAGEKSVEMIHVKDINKITGYIR
                     GGCTAIGMKKDYPTVLDESANAQEKIIVSGGKNGIQIELTTEDYLKACKGKTADITLR
                     "
     gene            complement(2216..2602)
                     /locus_tag="PLC71_01010"
     CDS             complement(2216..2602)
                     /locus_tag="PLC71_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01257.1"
                     /translation="MQKDFIYENYLKMPDDLEFEQAMKVYEELLEENLEEDEIYDKLW
                     DHALHCMIDYGSLRAHWKITPKTDRSNDDRTVMHDSVIHSLDELAAYTKEHGKEAKWR
                     EELGYQRKRIGDFACYVSLIYGVFAR"
     gene            2707..3966
                     /locus_tag="PLC71_01015"
     CDS             2707..3966
                     /locus_tag="PLC71_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRM01258.1"
                     /translation="MPEIQTNNLVDHGQLKIQVTSRQRAVPIPNATIEISYTGDPDSV
                     LETVSTDENGQTPVVDLPAPPVEYSMSPSENQPYSEYNLKIHSDEYKPVTISGAQILS
                     GVEGLQPVSMIPEETHTPTEEHPIVIGPHTLWGNYPPKIAESEIKPVNESGEIVLSRV
                     VIPEYIIVHDGPVGDKTAQNYYVRYKDYIKNVAACEIYSTWPRATLEANILAIMSFTL
                     NRVYTEWYRNKGHDFTITSSTAYDHKFIPGKTTYNSINTIVDEIFADYLSRPNVRQPI
                     LTQYCDGKKVSCPEWMTQWGSKYLGDQGYAPIEILRYYYGESMYINTAEQISGIPSSW
                     PGYDLTIGSRGDKVRQIQQQLNRIAKDYPSLPTIAVDGVYGESTANAVRKFQNIFGLP
                     QTGIVDYPTWYKISEIYVGVSRIAELN"
     gene            complement(4030..5688)
                     /locus_tag="PLC71_01020"
     CDS             complement(4030..5688)
                     /locus_tag="PLC71_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM01259.1"
                     /translation="MKNLNIPVGISDFERIRELNYYYVDKTGLIKTLLQGEMDQVTLI
                     TRPRRFGKTMAMNMLNSFLDIRKDSRHLFKGLEISDEVEICEKWMNQYPTLFLSFKDV
                     DGTTFENAFNFLKFIISEACKQHVYLLDSDEIDEGDKLVFQKLKTCSASMMEIQSCIL
                     RITNMLKSYYKKPVILLIDEYDVPIAKASSNGYYKEMLEIMKGLLSTALKDNSSLKFA
                     VITGCLKIAKESIFTGTNNFVSDTISSTRYNEYYGFTQQDVDKLLADAEIEEKADLIK
                     EWYDGYNFGEFEVYCPWDVMNYLRDLQNDLNARPVSYWKNTSDNAIIRSFIDYTGAAI
                     RKKLEVLIAGGSICQKIEEDLTYDYLHSSEDNLWSILYLTGYLTKVGERDKDGQIELR
                     IPNKEVKEIFESTVRKWFEDSARVTNRKDLFDAVWNKDADKATKEISTLLRMTISYYD
                     YRENFYHAFLAGIFAGAGYSVESNREHGEGRSDIVIYNDVTGQVAVFEAKYSRKLEDL
                     EKDCQKALDQINTKMYAKEFEDAYEEVLCYGIAFYKKRCLVKSK"
     gene            complement(5820..6524)
                     /locus_tag="PLC71_01025"
     CDS             complement(5820..6524)
                     /locus_tag="PLC71_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRM01260.1"
                     /translation="MNQSKLKLTTIIALTAFTVAVIPACLQRKSANAASKYTYKKGFT
                     YEKISPNIEKRITGKSYRKNKNIKLSDLRYVQVLHYGFDGKVKEGELIVNKKIAKKTV
                     KVFYALYQKKYRIERMRLIDDYGANDEKSMAANNTSAFNYRVISGTTKLSNHSYGMAI
                     DINPRINPWVKGNKVSPANGKVYKQRKTSKCKGKYKRYMIHKNDTAYKIFKKYGFSWG
                     GEWRSSKDYQHFEVNK"
     gene            6685..6813
                     /locus_tag="PLC71_01030"
     CDS             6685..6813
                     /locus_tag="PLC71_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01261.1"
                     /translation="MNTPALSEWLVVRPVTNLVQLRENPNANGSLGNYDLKYSLKE"
     gene            complement(6815..7300)
                     /locus_tag="PLC71_01035"
     CDS             complement(6815..7300)
                     /locus_tag="PLC71_01035"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRM01262.1"
                     /translation="MNLIVNADKNWGIGKNNELLVHIPNDMKMFRQMTTGKVVVMGRK
                     TLESFPNGMPLPKRTNIVLTHDKDYDAKGAIVVGSKEELLDELEEYKDEDIFIIGGES
                     IYRMMLPYCDTAYVTRTDFAYDADTYFPDLDEMPEWKLVKESEEETYFDIEYQFLVYQ
                     K"
     gene            complement(7316..8164)
                     /gene="thyA"
                     /locus_tag="PLC71_01040"
     CDS             complement(7316..8164)
                     /gene="thyA"
                     /locus_tag="PLC71_01040"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRM01263.1"
                     /translation="MSYADQVFISMCEDILENGTSTEGEKVRPHWPDGESAYTIKQFG
                     VVNRYDLSKEFPALTLRKTYIKSAIDELLWIWQKKSNNVNDLKSHIWDEWADEDGSIG
                     KAYGYQMGVKHQYKEGMMDQVDRVIYDLKHNPYSRRIMTNIYVHQDLHEMNLYPCAYS
                     VTFNVTKKPGHDKLTLNAILNQRSQDILAANNWNVVQYAALVYMMAQVCDMEPGELVH
                     VIADAHIYDRHIPIIKEMIKRETYPAPKVTLNPEVKDFYEFTTDDFKIEDYQAGEQIK
                     DIPIAI"
     gene            complement(8161..9306)
                     /locus_tag="PLC71_01045"
     CDS             complement(8161..9306)
                     /locus_tag="PLC71_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRM01264.1"
                     /translation="MKRWEIYLDIIMELLVILAGALLLIFVLPKSIVFLWPFVAGWII
                     SMMAHPVIEYLEKKVKLPKKFGSAILIAAVITGIIVVLYFAVRGIALQVIGFIQDAPD
                     IRHEIMRQGMIFQKKIEQFLSILPPSFGKQFDQWSGSIGELFKKAASSAGDYGVAHAG
                     GVAKGVTSGLLGLVVMLFAAYMFSLEREKLIAWYEKCIPGFVKHKINVFMKNSVGVLV
                     SYCLAQLKIMIVIIAILWIGFFIAGIGHSFIYSVLVGIVDIFPILGTGTVIIPWAIFK
                     LITGDIKTAVILLIIYAICLVLKQVLQPKMMGDSMGISALTTIFLIYVGLKFGGLGGM
                     LLALILGMFVINLYRLGVFDRKIAFFKRRFEMLTINSEEEEKENKKE"
     gene            complement(9396..10277)
                     /locus_tag="PLC71_01050"
     CDS             complement(9396..10277)
                     /locus_tag="PLC71_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5688 family protein"
                     /protein_id="HRM01265.1"
                     /translation="MLNEKEFYQYVEANILNYLPEMEGKKVMVQRVKRNNQISMMGLS
                     IGETKNYLVPVLYLEIFYRNYLRGQELSDTMKDIARIYRGHQVGFYLDEDKVSDYEHI
                     KKNLFYRVVNYEKNKEMLKYTPYERFLDLAVTYRWAAYRNHDGMASALVRNKELLLWG
                     VTKEQMMKDAKENTEKIFPPVMRKIQSVIPVKITDMEIPLFVLSNGDYMNGASVMLYK
                     DPLRDFANYMGHDLYILPSSIHEVILLLDDEYVQSSEELREMVRETNRMVVDQEEVLS
                     DHIYHYDREKDEIRIAK"
     gene            complement(10446..11501)
                     /gene="hisC"
                     /locus_tag="PLC71_01055"
     CDS             complement(10446..11501)
                     /gene="hisC"
                     /locus_tag="PLC71_01055"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRM01266.1"
                     /translation="MNSWRDNVRKVEPYTPGEQPKEEGVIKLNTNENPYPPSDKIKEA
                     MSGIKNLRKYPDPAATKLVEAIASYHGFNKEEVFVGVGSDDVLAVAFMTFFNGKKPIF
                     FPDITYSFYPVWAELFGIPYEKKAVNDAFEIQKEDYYAENGGVVFPNPNAPTGAFLSL
                     EVVEDIIQHNQDVVVIVDEAYIDFGGDTAKELTRKYDNVLVVQTYSKSRSLAGLRIGY
                     AIGNKELIKAMNDVKYSYNSYTMNEPSIVLGTAVLEDEEYFQETRNKIIDTREWFQIE
                     MRKIGFSFADSKANFIFATHESVPAKEIFEAAKKAKIYVRYFDQPRINNYLRITIGTR
                     DEMETLLDFLKEFTATK"
     gene            complement(11528..12718)
                     /locus_tag="PLC71_01060"
     CDS             complement(11528..12718)
                     /locus_tag="PLC71_01060"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRM01267.1"
                     /translation="MLSKEVVELSKQHSIIRDIFEYGMKRAKEVGAENVFDFSIGNPS
                     VPAPKEIDETALRLLKTADPVDIHGYTSNAGVLEVRENIAKSLNKRFDTNYTAANIFM
                     TIGAAAALGICFKTLVDGPEDEVITFAPHFPEYAVYAQGAGCRLKVISADTRAFQINF
                     EELEETINEHTKAILINSPNNPSGVVYSRETIEKLAALLEKKQKEYGHSIYIISDEPY
                     REIAYDGFEVPYVPHFYNNTLVAYSFSKSLSLPGERIGYIVAPNEAEDFEQLLPAFIA
                     SARLLSYVCVPTLYQKVVGECVDLTSDLSIYQKNKDLFYNALVDMGYECVEPGGAFYL
                     FPKALEEDANAFCEKAKEYDLLIVPGDSFGCPGYVRISYCVPTERIEKALPLFKKLID
                     DYRK"
     gene            complement(12832..13620)
                     /gene="trpA"
                     /locus_tag="PLC71_01065"
     CDS             complement(12832..13620)
                     /gene="trpA"
                     /locus_tag="PLC71_01065"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRM01268.1"
                     /translation="MKIRETFENRKKNGQKALVTYIVSGDYGYETTKENIRAMVKAGA
                     DVIEIGIPFSDPIAEGVVIQEASQHSLAGGTTLKGIFQMVKELREEIKETPFVMMMYL
                     NTIYRFGTERFFDLCNECGIQGVIVPDMPYEEKDEIQGVATAHGVDNISLVTPTSHDR
                     IAMIAKEAEGFLYCVSSIGVTGTRSEFTTDFDEFFGVIKKNATIPCAVGFGISGPEQA
                     KKMSTYCDGVIVGSAIVKLISQYGKESPEKVYEFTKSLRDALDE"
     gene            complement(13610..14800)
                     /gene="trpB"
                     /locus_tag="PLC71_01070"
     CDS             complement(13610..14800)
                     /gene="trpB"
                     /locus_tag="PLC71_01070"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRM01269.1"
                     /translation="MNKIGRFGEYGGQYVPEIVMNAVNELNEAYETYKNDPEFLAQLR
                     QLYRDYANRPSLLYYAEKMTKDLGGAKVYLKREDLNHTGAHKINNVLGQILLAKRMGK
                     KRIIAETGAGQHGVATATVAALFDMECVVYMGAEDVERQSLNAYRMELLGAKVHAVES
                     GTRTLKDAINEAMRDWATNIETTFYCIGSVMGPHPYPTMVRDFQKIIGEETKEQMKEI
                     EGKLPDAVFACVGGGSNAMGMFYDFIPDESVRLIGAEAAGRGIDTLDHAATIAKGSKG
                     IFHGMKSLFLQNEEGQIDPVYSISAGLDYPGIGPEHAHLHEIGRAEYVSITDEQAVKA
                     FEYLSKTEGVIPAIESSHAVAAAMEIVPTMSKDQSVVICLSGRGDKDVYQVARYRGVQ
                     LDEN"
     gene            complement(14793..15452)
                     /locus_tag="PLC71_01075"
     CDS             complement(14793..15452)
                     /locus_tag="PLC71_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRM01270.1"
                     /translation="MIQNQIKERSLKVKMCGMRRKEDIAYANEVKPDAIGYIFFSKSK
                     RYVTGQQARELDQNLDQKILSVGVFVNETIEKVTEIANEVPLDVIQLHGDEDVIYIEQ
                     LRQQTDKEIWKAVRVKDTKDIKEAQQLPVDKLLLDTFIEGKDMYGGTGKVMNYDLIPK
                     EGIRKPFFIAGGLHSKNIKEITEKVHPYGIDISSGIETDGYKDLKKMKEIMQITGGRH
                     E"
     gene            complement(15449..16228)
                     /gene="trpC"
                     /locus_tag="PLC71_01080"
     CDS             complement(15449..16228)
                     /gene="trpC"
                     /locus_tag="PLC71_01080"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRM01271.1"
                     /translation="MILDDIVEKRKEQLQREKDNIEPQDMKEMALNSKNKNHGFKEAL
                     KKSGLSVISEVKKASPSKGVIAEDFRPVETAIAYEDAGAAAISCLTEEHYFKGGSKYF
                     ADIRAKVDIPMLRKDFIFDEYQIYEAKVLGADAILLIAAILSEEKIKEFYDLAKSLEI
                     DCLVEVHNEKELKKVVACGCDIIGINNRNLKTFDVDLNTTSKLAPLIPYEAVLVSESG
                     MKDENDMKNVKEQGADAVLIGETFMRSDNIKETMKQLRSCL"
     gene            complement(16241..17884)
                     /locus_tag="PLC71_01085"
     CDS             complement(16241..17884)
                     /locus_tag="PLC71_01085"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component
                     II/anthranilate phosphoribosyltransferase"
                     /protein_id="HRM01272.1"
                     /translation="MILMIDNYDSFTYNVYQYIGSLYPQIQVVRNDEITIDEIRNLQN
                     LEALVISPGPGYPDSAGISKEAIKTFGKEIPVLGICLGHQAIGEVYGGKVVPAKELMH
                     GKMSEITINNKNPLFEGLEDKIYAARYHSLIIDDETFPEDLKVIGRDEKGQIMAVCHK
                     EYPVYGIQFHPESILTEMGMRILENFLTNIAGIRLGDSKKEETMSAVNQETLKPFLTK
                     IVEGNHLTEEEAYKAMDCIMSGNATDAQMGSFLTGLRMNHETPEEITGFAKVMRAKAA
                     IVPEETEAIDIVGTGGDLANSFNISTTSAFVIAAAGAKVAKHGNRSVSSKSGAADVLE
                     ALGAKIGLTPEESKKCLDKVGAAFLFAQTHHGSMKYAGPVRAQLGVRSVFNILGPLAN
                     PAMTNYIVLGVYEKELVRPMADVMKNLGVKRALIVYGDDGLDEISISSTTSVCEINGD
                     EIKEYTIDPEELGLTLAKKEDIVGGTADENAIITKDILTGKEQGAKRDIVLLNAGAAL
                     YTIGKAETIKDGVKLAAEMIDSGKANEKLEQFIAYTNVK"
     gene            complement(17881..19338)
                     /gene="trpE"
                     /locus_tag="PLC71_01090"
     CDS             complement(17881..19338)
                     /gene="trpE"
                     /locus_tag="PLC71_01090"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRM01273.1"
                     /translation="MLYPTLNKVEELLKEYQMVPVFYEVLADYMTPIRMFQALRKEGT
                     PCFMLESVENKDQWGRYSFIGINPKSEIKISGKELEVDGVKQEEEFKMSYLSDLIEKY
                     KSPVMEDYPKLTGGLIGYFGYDMIRQVEKKLTNVPEDDLKMPECHLCMYDEIIAFDHL
                     ANKAVIIQNIHKGDNIKQKYEELEDKAELILHKMERPVSLSKDRFTPAKAEVTSNLTK
                     EQYEANVKKAKEYIKNGDIFQVVLSQRFEVETDVDPFDVYRCLRTSNPSPYLYFFDFI
                     DYQVVGASPEKLVSVLNGIVATKPIAGTVPRGKTKEEDDMLVRQLVNDPKERAEHTML
                     VDLGRNDVGKVSKFGTVEVKNFMTVEKYSKVTHLVSDVQGELRDDENPINALMSVLPA
                     GTLSGAPKVRAMEIIDELENKKRGVYGGTVGYLGFDGNIDTCIAIRTVVFKDGKGYVQ
                     AGAGIVNDSVPEKEFMETKNKALAVVNAVKEAARL"
     misc_binding    complement(19485..19788)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         5342 a         4554 c         3015 g         7133 t
ORIGIN      
        1 catcccaagg actcctccaa ctacaatgat cagaatcgcg aactgataca ttcctaaaaa
       61 caatcctaaa attccaagga tccatcctag acttaatccc aaagatctca ttcctgcgat
      121 ctgacagctt tgaaaccaat tgatatccgc ttctgccatc gctttcatta atcccatgac
      181 aagtgctgcg atcacaagtg atacgatgat cgcgataatt catttttctt cattcttttc
      241 tcctcttctc tcgtaatacc ggtcaacacc atctgcaatt ccttctgcaa gcattgattg
      301 ataatgattg tccgataact tctggtcttc ctctgggttt gacataaatc ccatctcaac
      361 gatcgtcact ggaatcttac tccagttgat ccctgtcatc tcatctgtct gcgttacacc
      421 gcggttcact gccccagtcc ttctgcataa tgtacttacc acacattctg aaagttcctg
      481 actatctgcc gcgatcgatc tgcaatatgg attcgataat gtcggactca ttgtcaacgc
      541 cccatttgca gactgagaat ctgcactatt gcaatgaatc cttaataaaa tatctgaacc
      601 actttcattt gccataagag cacgcttttt attgctgagt gacacatcat tcgtctcacg
      661 gatcatatat acgtcatatc ctctcgcgat caatgcactt tttagcttta acgaaacttc
      721 caaattcacc tgatattctg ttcgttttgt gacaatacct tcggttcctg aggataccat
      781 cggttttgtc ttatcagaac caggtccgat cgcttctttt tcactcatct gtttcttttg
      841 gtgtcctgca tcgattgcga tctttatacg atcttcctgt tgttttgatt gttcctgatc
      901 aaccgttact tcattctttt tttgtgttgt cttttgcttg atgatctctg ttcttttttc
      961 tgtcgttggt tttggatgat cttcactgat catttcatca tcattgctgc atccagcaca
     1021 gaaaaaacaa aggatcatac ataagatcat gatcttccat atctttttca taccttttat
     1081 ccctctcatt tataatgctg cagttgttct atggctctac aacatgaagc tctcctgtca
     1141 cagaatccat cacatatcca gatatcttca catcttttgg aatcaatgga tgttttctca
     1201 acatttccac agaatctttt acagaacttt ccacacagtc aaatccttcc agccatgtat
     1261 caaaatcaat accacaatga cggatcagct caatcttctc ctcatcgact cctcgatcca
     1321 tcatatgttt tatcatcata tcactattaa tatgctgtac tccacagtca gtgtgtccaa
     1381 tgaccatgac ttcttcaatc ccaagttcaa agattccgac taaaacactt cgtaccacac
     1441 ttccgaaggc atggcttact accccaccgg cattcttaat gatcttcaca tctccattcc
     1501 tgatcccaag ggctgccggc aaaagttctg tcagtcttgt atccatacaa gtgatgattg
     1561 cgattttctt gtctggatat ttgcttgtct tatattgttc atacttcttt tcttctacaa
     1621 attctttgtt atactttaag atttcatcta tcataacttc tactccctac ctcaatgtaa
     1681 tatctgccgt ttttcccttg caggctttta aataatcttc ggtcgtaagt tcaatctgga
     1741 ttccattttt accgccgctt acaatgatct tctcctgtgc attggcgctt tcatcgagca
     1801 ctgttggata gtctttcttc atcccgatcg ctgtgcatcc accacggata tatcccgtga
     1861 tcttgttgat atccttgaca tggatcatct ccacagactt ctcacctgct gccttcgctg
     1921 ctttcttaag atctaattcc tgatcgatcg gaatgacaaa tacaaaatac cctccacctt
     1981 ttccttcagt cacgagggtt ttaaatactc tttcatatgg ttcatttaac atatctgcga
     2041 tggtaattcc atcaataaat tctttacatt cataacgatg tacttcaaat gggatctttg
     2101 ctctctcaag catccgcatt gcatttgttt ttggttcttt ctttgccatc ttataatgtc
     2161 ctccttactg atcgttttat ctggcaaaat ttacttttga cactctcatc atatttcacc
     2221 ttgcaaatac tccatagatc aaagaaacat aacatgcaaa atctccgatt ctttttcttt
     2281 gatatccaag ttcttctctc cattttgctt ctttgccatg ctcttttgta tatgctgcca
     2341 attcatctaa agaatggatc acgctatcgt gcatcactgt cctgtcatca ttgcttctgt
     2401 ctgtcttcgg tgtgatcttc caatgtgcgc ggaggcttcc ataatcgatc atacaatgaa
     2461 gtgcatgatc ccataacttg tcatagatct catcttcttc aagattctct tctaacagtt
     2521 cttcataaac cttcattgcc tgctcaaatt caagatcatc tggcattttt aaataattct
     2581 catatataaa atctttctgc atagtggtct cctttatctc tttggcatac atttttacac
     2641 tacttgtgca cattatatca tattttctca tatagtgaga gtaaaagatc ttccgaggat
     2701 atttttatgc cagaaataca aactaacaat cttgtcgatc atggacagtt aaaaatacag
     2761 gtaacttcaa gacaaagagc cgtcccaatc cctaacgcta cgatcgagat ttcttatact
     2821 ggtgatcctg attctgttct tgagactgta tctactgatg aaaatgggca gacacctgtc
     2881 gttgacctcc ctgctcctcc cgtggaatac agcatgtctc cgagtgaaaa tcagccttat
     2941 tctgaatata atctaaaaat acattctgat gaatataaac ctgtcacgat ctcaggtgca
     3001 cagattcttt ctggtgtgga aggcctgcag cctgtttcca tgattcctga agaaacccac
     3061 actccaacag aagaacaccc gatcgtgatc ggtccgcata ccttatgggg taattaccct
     3121 cccaagattg cggaatctga gatcaagcca gtcaacgaaa gtggtgagat cgttttaagc
     3181 agggttgtga ttcctgaata tattattgtt catgatggtc ctgtcggtga taaaactgcg
     3241 caaaattatt atgtccgcta taaagattac atcaaaaatg ttgccgcctg tgagatttat
     3301 tccacttggc caagggcaac gcttgaagca aatattcttg cgatcatgtc atttactttg
     3361 aatcgtgtgt atacagaatg gtatagaaat aaagggcatg attttacgat cacatcttct
     3421 acagcttatg atcataaatt cattcctgga aagacaactt ataattctat taatacgatc
     3481 gttgatgaaa tttttgcaga ttatctgtct cgtccgaatg tgagacagcc gatccttacg
     3541 cagtattgtg atgggaagaa ggtgtcttgt cccgaatgga tgacacagtg gggttctaaa
     3601 tacttaggtg atcagggata tgctccgatt gagatcttga gatattatta tggagagagt
     3661 atgtacatca ataccgccga acagatttca ggaattcctt cttcctggcc gggatatgat
     3721 ctgacgattg gaagcagagg agataaggtt cgacaaatac aacaacaatt gaatcgtatt
     3781 gctaaagatt atccttctct gcctacgatc gctgtggatg gtgtttatgg agaatctact
     3841 gcgaatgctg ttcggaaatt ccagaatatt tttggactgc cacagacagg gattgttgat
     3901 taccctactt ggtataagat cagtgagatc tatgttgggg ttagccggat tgcggagttg
     3961 aattgaaatc ttagatacaa caaaagcccc acaaagttcg tccttgtgga gcttttttca
     4021 tttcttattc tatttactct tcaccaaaca ccgcttttta taaaaagcaa ttccataaca
     4081 caatacttcc tcataagcat cttcaaactc ttttgcatac atctttgtgt taatctgatc
     4141 taacgccttt tgacagtctt tttccaaatc ttctaatttt cttgaatact ttgcttcaaa
     4201 tactgcaact tgtcctgtta catcattgta gattacaata tcacttcgtc cttctccatg
     4261 ctctctattc gactctacac tataacctgc tcctgcaaag attcctgcaa gaaatgcatg
     4321 atagaaattt tctcggtaat cataataact gatcgtcatt cgcagtaatg tgctgatttc
     4381 ttttgttgcc ttatccgcat ctttgttcca cactgcatca aataaatctt tccgattcgt
     4441 aactcttgca ctgtcttcaa accatttcct tacggttgat tcgaaaattt cttttacttc
     4501 tttgtttgga attcttaatt ctatctgacc atccttatca cgttctccga cttttgtaag
     4561 atatccagta agatagagaa tactccataa attatcttct gatgaatgta gataatcata
     4621 tgtgaggtct tcttctattt tctggcagat gcttccacct gcgatcaata cttctagttt
     4681 ttttcgtata gcagctcctg tataatcaat aaaggagcga atgatcgcat tatcactggt
     4741 attcttccag tagctgactg gtcttgcatt cagatcattt tgaagatctc ttagataatt
     4801 catgacatcc catggacaat aaacttcaaa ctctccaaaa ttatacccat cataccattc
     4861 tttaatcaga tccgctttct cttcaatctc cgcatctgct aaaagcttat ctacatcctg
     4921 ttgtgtaaat ccataatatt cattgtatct tgtggatgaa atcgtatctg atacaaagtt
     4981 atttgttcca gtgaaaatac tttctttggc aatctttagg catcctgtga tgactgcaaa
     5041 ctttaatgag gaattatctt ttaaggctgt actaagcaaa cctttcataa tttctaacat
     5101 ctctttgtag taaccattac tacttgcttt tgcaattgga acatcatatt catcaattaa
     5161 aaggatcact ggctttttat aataactttt cagcatattg gtaattctta atatacaact
     5221 ttgaatttcc atcatacttg cactacaagt ctttaatttt tgaaatacca gtttatcccc
     5281 ttcatctatc tcatcactat ccaacaagta tacatgttgt ttacatgctt cagatataat
     5341 aaatttcaaa aaattaaacg cattctcaaa tgtagttcca tctacatcct taaaagacaa
     5401 aaataatgtc ggatactgat tcatccattt ctcacaaatc tcgacctcat cagatatctc
     5461 aagtccttta aacaaatgtc tgctatcttt cctgatatcc aaaaaactgt tcaacatatt
     5521 catcgccatc gtttttccaa accgcctagg ccgcgtaatc aacgtcacct gatccatctc
     5581 accctgtaac aacgtcttga tcaatccggt cttgtccaca taataataat tcaactctcg
     5641 aattctctca aaatccgaaa tcccaacagg aatatttaga ttcttcatct cttcctactg
     5701 cctcctttct tttattaatt atatattgat aaacatatat tcgcagttca attatgttaa
     5761 atctttaaac caatacatgt tatagccaac aaccttccag aatcaatagt taatcatttc
     5821 tatttattca cttcaaaatg ctgataatcc ttagagcttc tccactcacc accccagctg
     5881 aatccatatt tcttaaagat cttataagct gtgtcattct tatggatcat atatctctta
     5941 tattttcctt tacactttga cgtctttctc tgcttatata ccttaccatt tgccggtgat
     6001 accttatttc ctttgaccca cggattgatc cttggattga tatcgatagc cattccataa
     6061 gaatggttgg aaagtttcgt cgttccactg atcacacgat aattaaacgc agaggtatta
     6121 tttgcagcca tagacttctc atcattcgca ccataatcat cgatcaatct cattctctca
     6181 attctgtatt tcttctgata aagagcataa aagaccttga ccgttttctt cgcgatcttc
     6241 ttgttaacga tcaactcacc ctcttttacc ttcccatcaa aaccataatg aagaacctgt
     6301 acatatctta aatcagacaa cttaatattc ttattctttc gataagattt tcccgtgatc
     6361 ctcttttcaa tattcggaga aatcttctca taagtaaagc cttttttata agtatatttc
     6421 gaagctgcat tcgcagattt tctctgtaaa caagccggaa tcacagccac tgtaaacgct
     6481 gtcaatgcaa ttatcgtagt taatttcaat tttgattgat tcatttattt tcatcctttc
     6541 caaagcttta atatttatct taatcgattt cattataaca tatattccaa agccccacac
     6601 tccatacctt gaagcatttc aaaacaacaa ttcaatatcc tcttgtataa agaatatttt
     6661 aaatcataat tgcccagtga ccgtatgaac acgcctgcac ttagtgagtg gctggtcgta
     6721 agacctgtca cgaacttagt acagctgcgt gagaatccga atgcgaatgg gtcactgggc
     6781 aattatgatt taaaatattc attgaaagag tgaattattt ttgatataca agaaattgat
     6841 actcaatatc aaagtaagtc tcttcttcac tctctttcac aagtttccac tctggcatct
     6901 catccagatc tggaaaataa gtatctgcat catatgcgaa gtctgttctt gtaacataag
     6961 ctgtatcaca ataaggaagc atcatgcggt aaatgctttc tccaccaatg ataaaaatat
     7021 cttcatcctt atattcttcc agctcatcta acaattcttc tttacttcca actacgatcg
     7081 ctccttttgc atcgtaatct ttatcatgtg ttaatacaat attcgttctc tttggaagcg
     7141 gcattccatt tggaaagctt tctaatgttt ttcttcccat gacaacgact tttcctgttg
     7201 tcatctgacg aaacatcttc atatcatttg gaatatggac taaaagttca ttgttctttc
     7261 caattcccca gttcttatct gcatttacaa taagattcat cttatcttct ccttcttata
     7321 ttgcaattgg aatatctttg atctgttctc ctgcctgata gtcttcgatc ttaaagtcat
     7381 ctgttgtgaa ttcatagaaa tctttgactt ctggattaag cgttactttc ggtgcaggat
     7441 atgtctctcg tttgatcatc tctttgatga ttggaatatg gcgatcataa atatgtgcat
     7501 ctgcgatcac atgaactaac tctcctggtt ccatatcaca tacttgtgcc atcatataaa
     7561 ctaatgctgc atactgtaca acattccagt tatttgctgc taaaatatcc tgagaacgct
     7621 ggttcaagat tgcattcaat gtgagcttat catgtcctgg tttctttgtt acattaaatg
     7681 tcacactata cgcacatgga taaagattca tctcatgaag atcttggtgg acataaatat
     7741 ttgtcatgat ccttctactg tatgggttgt gtttcagatc atagatcaca cgatctacct
     7801 gatccatcat tccttctttg tattggtgct ttacacccat ctgatatcca taagcctttc
     7861 caatcgaacc atcttcatct gcccattcat cccagatatg actcttcaga tcatttacat
     7921 tatttgattt cttctgccag atccataata attcatcaat agctgactta atatatgtct
     7981 ttcttaaagt caatgctgga aattctttgg acagatcgta acgatttaca acaccaaact
     8041 gtttgatcgt ataggcagac tctccatctg gccagtgcgg acggactttc tctccctctg
     8101 tactagttcc attctctaaa atatcttcgc acatagatat aaatacctga tctgcataac
     8161 tcattctttc ttgttctcct tttcttcttc ctcgctgttg atcgtaagca tctcaaatct
     8221 tcgtttaaaa aatgcgatct tacggtcaaa cactcctaat cggtataaat tgatcacaaa
     8281 cattcctagg atcagggcta ataacattcc tcctaatcct ccgaatttca gaccaacgta
     8341 gatcaaaaag atcgttgtca gtgctgagat tcccatactg tctcccatca tctttggctg
     8401 caagacctgt ttcaatacca gacagatcgc ataaatgatc agtaagataa ctgctgtttt
     8461 gatatctcct gtgatcagct taaatattgc ccatggaatg atcacagtcc ctgttccaag
     8521 gattggaaat atatcgacaa ttcctactaa taccgaatag ataaaagaat gtccaatccc
     8581 tgcaataaaa aatccgatcc aaaggatcgc tatgatcact atcatgatct ttaactgcgc
     8641 aaggcagtaa cttactaaga ctccaactga gttcttcata aatacattga tcttatgctt
     8701 cacaaatcct ggaatacatt tttcatacca tgctatgagt ttttctcgtt ccagtgaaaa
     8761 catataagct gcaaataaca taacaacaag ccctaacaga ccgcttgtca caccctttgc
     8821 aacaccacct gcatgtgcta ccccataatc tcctgcagaa gatgccgctt tcttgaaaag
     8881 ctctccgatc gatccgctcc actgatcaaa ctgttttcca aaagatggtg gaagaatact
     8941 taaaaattgc tctatcttct tctgaaagat cattccttgt ctcatgatct catgacggat
     9001 atctggtgca tcctgtataa atccgatgac ctgcagtgcg atacctctga ctgcaaaata
     9061 aagaaccact ataattcctg tgataactgc cgcgatcaag atcgcagaac cgaacttctt
     9121 cggtaatttt accttcttct ccagatattc gatcactggg tgggccatca ttgaaataat
     9181 ccatcctgct acaaatggcc ataaaaatac gattgatttt ggtaatacaa agatcaaaag
     9241 caatgcacct gctaatatga ccaacagttc catgataata tccagataaa tttcccaacg
     9301 tttcataatt ttttcactcc ctgatgtaat tagtatattc ttgaattttc ataccatttt
     9361 ccatctctta atgaactatt atacgttaca aattcttact tggcaattct aatctcatct
     9421 ttctctctgt cgtaatgata tatgtgatct gataaaactt cttcctgatc cacaaccata
     9481 cggtttgtct ctctaaccat ttctcgtagt tcctctgaac tttgcacata ttcatcatcc
     9541 agtaaaagga tcacttcatg aatactgctt ggaagaatat aaagatcatg tcccatataa
     9601 ttcgcgaaat ctctcagtgg atctttataa agcatgacgg atgctccatt catataatct
     9661 ccatttgata atacaaacaa tggaatctcc atatccgtga tctttactgg tatcacagac
     9721 tgaatcttgc gcatcactgg tgggaagatc ttctccgtgt tctcctttgc atccttcatc
     9781 atctgttcct ttgtcacacc ccataacagc aactctttgt tcctcactaa tgcactcgcc
     9841 atcccgtcat gattccggta tgctgcccaa cgatatgtaa ctgccagatc taagaaccgc
     9901 tcgtatggtg tatactttaa catctcttta ttcttctcat aattaaccac acgataaaac
     9961 agattcttct taatatgctc ataatctgaa actttatcct catccagata aaatccaacc
    10021 tgatgtcctc gataaattct ggcgatatct ttcatcgtat ctgataattc ctgtcccctt
    10081 aaataatttc gataaaatat ctccaaatac aacactggta ccagataatt cttggtctct
    10141 ccgatcgaca accccatcat cgatatctga ttattcctct taaccctctg caccataact
    10201 ttctttcctt ccatctctgg aagatagttt aaaatattcg cttctacata ctgataaaat
    10261 tctttctcat tcaacatggt cttctccttt tctgtggatg aaaacgcatg ccataacccc
    10321 attttatttg tttccctcat ttgtcttcat atgtaaataa tttccatatc atcatacaat
    10381 attttccagt tgattgcaat aaaaaaagaa atttattttt caaaatttct ttttttattc
    10441 aatctctatt ttgttgctgt aaattctttt aagaagtcaa gtaatgtttc catttcatct
    10501 ctcgtaccga tcgtaatcct taaataatta ttgattcttg gctgatcaaa ataacggaca
    10561 taaatctttg ctttcttcgc tgcttcaaaa atctctttgg caggtacgct ttcatgtgtc
    10621 gcaaagataa agtttgcttt ggaatctgca aaactaaatc cgatctttct catctcaatc
    10681 tggaaccatt ctcttgtatc aatgatctta tttcttgtct cctggaaata ttcttcatct
    10741 tctaagacag ctgttccaag aacgatggat ggctcattca tcgtataaga gttgtaggaa
    10801 tatttcacat cattcattgc cttgatcagt tccttgttac cgatcgcata tccgatacga
    10861 agtcctgcta aagatcttga tttggaatat gtctgaacga ctaaaacgtt atcatatttt
    10921 cttgtcagtt ctttcgctgt gtcgccgcca aaatcaatat aggcttcatc aacgatcaca
    10981 acaacatcct gattgtgttg gatgatatct tccacaacct ctaaagataa aaatgcgcct
    11041 gttggtgcat taggatttgg gaataccacc ccgccatttt ctgcgtaata atcttctttc
    11101 tgaatctcaa atgcatcatt gactgctttc ttctcatatg gtattccgaa taattctgcc
    11161 catacaggat agaaggaata tgtgatatct gggaagaaga ttggtttctt accgttaaaa
    11221 aatgtcataa atgcaacggc aagcacatca tcagatccaa ctccgacaaa tacttcttct
    11281 ttattaaatc catgataaga agcgatcgct tctaccaatt tagttgctgc aggatcagga
    11341 tatttgcgca aattcttgat cccactcatc gcttccttga tcttatcgga tggcggataa
    11401 gggttttcat ttgtatttaa cttgatcaca ccttcttctt ttggctgttc acccggtgtg
    11461 taaggttcta cttttcgtac attgtctctc cagctgttca ttgaaagtct cctttatcta
    11521 tattggctta tttgcgatag tcatcgatca gtttcttaaa taatggaagg gctttttcga
    11581 tacgttctgt tggtacacag taagaaatac gcacatatcc tgggcatcca aagctgtccc
    11641 caggtacgat caacagatca tattctttcg ccttctcgca aaatgcattg gcatcttctt
    11701 ctaatgcttt cgggaataaa tagaaagctc cacctggttc tacacattca tatcccatat
    11761 ccactaatgc attatagaat aaatctttgt tcttctgata gattgaaagg tcagaagtta
    11821 aatctacaca ttctccaaca actttctgat ataaggttgg cacacagaca taagaaagta
    11881 aacgtgcaga tgcgataaac gctggcagta actgttcaaa atcttctgcc tcatttggtg
    11941 ctacgatata accgatacgt tctcccggca gtgataagga tttactaaaa gaatatgcta
    12001 ctaatgtatt gttataaaaa tgtggaacat atggcacttc aaatccatca tacgcgatct
    12061 ctctgtatgg ttcatcggaa atgatgtaaa tgctgtgtcc atattctttt tgtttcttct
    12121 ctaataatgc agctaatttc tcaatcgtct ctctggaata aacaactccg gatgggttgt
    12181 ttggagagtt gatcaagatt gctttggtat gctcattgat cgtttcttct aattcttcga
    12241 aattaatctg gaatgctctg gtatctgcag aaatgacctt taatctgcat cctgctccct
    12301 gtgcatatac tgcatattct gggaaatgtg gtgcgaatgt gataacttca tcttctggcc
    12361 catcaactaa tgtcttaaag cagataccaa gggctgctgc cgctccgatc gtcataaaaa
    12421 tattcgctgc tgtataattt gtatcaaatc ttttatttaa agacttggca atgttttctc
    12481 ttacttccag tactccagca ttgcttgtat atccatgaat atccactgga tctgctgttt
    12541 ttaacaggcg aagtgctgtc tcatcaattt cttttggtgc tggaacactt ggattcccga
    12601 tactgaaatc aaatacattt tctgctccga cttcttttgc tctcttcatt ccatattcga
    12661 aaatatcacg gataatagaa tgctgtttcg ataattctac tacttctttt gataacatga
    12721 tctgtctctt cctttctttg ccattagttt tattttacaa aaaaatgcag aaaattacaa
    12781 caaccctcgc tgattgctgt aattttcacg taatatcatt tgacgatcat tttattcatc
    12841 taatgcatct cttaagctct ttgtaaactc ataaaccttt tcaggacttt ctttaccata
    12901 ttgactgatc aatttgacga tcgcacttcc gacaatgaca ccatcacagt atgtactcat
    12961 tttctttgcc tgttctggtc ctgaaatacc aaatcccact gcacaaggaa ttgttgcatt
    13021 cttcttgatc acaccgaaga attcatcaaa atctgttgta aattcacttc ttgttcctgt
    13081 aactccgatg gaggatacac agtataagaa tccttctgct tcttttgcga tcattgcgat
    13141 acggtcatga gatgtcggtg ttacaagact gatattgtca actccgtgag ctgttgccac
    13201 gccctgaatc tcgtcttttt cttcgtatgg catatcaggc acgatgacgc cctgaatccc
    13261 acattcatta caaagatcaa agaatctttc ggttccaaaa cgatagatcg tattgagata
    13321 catcatcatg acaaatggtg tttctttgat ctcttctctt aattctttta ccatctgaaa
    13381 gattcccttt agtgttgtac cacctgcaag tgaatgctgg ctggcttcct gaatgacaac
    13441 accctcagcg atcggatctg agaatggaat accgatctcg atcacatctg ctcctgcttt
    13501 taccatagct ctgatatttt cttttgttgt ttcgtaccca taatctccag atacgatgta
    13561 agttactaat gctttttgcc cattcttttt tctattctca aatgtctctc taattttcat
    13621 ctaactgcac tcctctgtat cttgctacct gataaacgtc tttgtctcca cggccagaca
    13681 gacagataac gactgactgg tctttactca ttgttggaac gatctccatt gccgctgcaa
    13741 ctgcatgaga tgattcgatc gctgggatca ctccttctgt tttggataaa tattcaaagg
    13801 ctttgactgc ctgttcatct gtgatactca catattctgc tcttccgatt tcatgaagat
    13861 gtgcatgttc tggtccgatt cccggataat caagtcctgc tgaaatagag tatacaggat
    13921 cgatctggcc ttcttcgttc tgtaaaaaca aactcttcat tccatgaaaa atacctttac
    13981 ttccttttgc gatcgttgct gcatgatcta acgtatcaat ccctcttcca gctgcttctg
    14041 ccccgatcag tctgacactt tcatctggga taaagtcata aaacattccc attgcattcg
    14101 atccgcctcc tacacaagca aatacagcat ctggaagttt tccttcgatt tctttcatct
    14161 gttcttttgt ctcttctccg atgatcttct ggaaatcacg aaccatcgtt ggatatggat
    14221 gaggtcccat aacagaaccg atgcagtaaa atgttgtctc aatatttgta gcccagtctc
    14281 tcattgcctc gttaatggca tcttttaaag ttcttgttcc actctctaca gcatgaactt
    14341 tcgctccaag aagttccata cggtatgcat tcagactctg gcgttccacg tcttctgctc
    14401 ccatatatac aacacattcc atgtcaaata atgctgcaac agtcgctgta gcaactccgt
    14461 gctgtcctgc acctgtctct gcgatgattc tttttttccc cattctcttt gcaagtaaga
    14521 tctgacctaa tacattgttg atcttatggg cacctgtatg gttcaaatct tctcgtttta
    14581 aatatacttt tgcccctcca agatcttttg tcatcttctc tgcgtagtat aaaagagatg
    14641 gacggtttgc gtaatctctg tataactgac gtaattgggc taagaattct gggtcatttt
    14701 tgtatgtttc ataagcctca tttaactcgt ttaccgcgtt cattacaatt tctggtacat
    14761 actgtccacc gtactctcca aatcttccta ttttattcat gtcttcctcc tgttatctgc
    14821 atgatttctt tcattttttt caaatctttg tatccatccg tttcgattcc actggaaata
    14881 tcgattccgt atggatgaac tttttctgtg atttctttta tatttttact gtgaagcccg
    14941 cctgcaatga aaaatggttt tctgattcct tcttttggaa tcaggtcgta attcattact
    15001 tttccggtac ctccatacat atcttttcct tctataaatg tatccagtaa cagtttatca
    15061 actggaagct gttgggcttc cttgatgtct tttgtatctt tcactctgac cgctttccag
    15121 atttctttat ctgtctgctg tcttaactgt tcaatataaa taacatcttc atcgccatgt
    15181 aactggatca cgtctaatgg aacttcattg gcgatttctg taactttctc aattgtttca
    15241 ttgacgaata ctccgacact taagatcttt tgatctaagt tttgatcaag ttctcttgcc
    15301 tgctggcctg tgacataacg cttgcttttt gaaaagaaaa tatatccaat tgcatctggt
    15361 tttacttcat ttgcatatgc gatatcttct tttctgcgca tgccgcacat cttcactttc
    15421 agacttcgtt ctttgatctg attttgtatc ataggcagct ccgtaactgt ttcattgttt
    15481 ctttgatgtt atcggatctc ataaatgttt caccgatcaa aactgcatcg gctccttgct
    15541 ctttgacatt tttcatatca ttttcatcct tcattccact ttcggaaact aatactgctt
    15601 cgtatggaat aagtggagca agtttacttg ttgtatttag atcaacatca aatgttttta
    15661 aatttcgatt attgattccg ataatatcgc atccacatgc aacgactttc tttaattctt
    15721 tttcgttatg aacttctacc agacaatcaa tctctaagct ttttgccaga tcataaaatt
    15781 cctttatctt ttcttcagaa agaattgctg cgatcagaag gattgcatcg gctcctaaga
    15841 cttttgcttc ataaatctga tattcatcga aaataaaatc ttttcttaac attgggatat
    15901 ctaccttagc tctgatatcc gcaaaatatt tgctgccacc tttaaaataa tgttcttcag
    15961 tcaagcagga aatggctgct gcccctgcat cttcataggc aatggctgtc tccactgggc
    16021 gaaaatcttc tgcgatcaca cctttggatg gagatgcttt tttaacttct gagatcacgg
    16081 ataatccaga tttctttaag gcctctttaa atccatgatt tttattctta gaatttaatg
    16141 ccatctcttt catatcctgc ggctcgatat tgtctttttc tctttgtaat tgttcttttc
    16201 ttttttctac gatatcatct aagatcatgt tccacctcat ttattttaca tttgtgtatg
    16261 caataaactg ttctaatttc tcatttgcct ttccagaatc gatcatctcg gctgccagtt
    16321 tcactccatc tttgatcgtc tctgcttttc cgatcgtata aagggctgct cctgcattta
    16381 ataaaacgat gtctcgtttt gctccctgtt cttttccagt taggatatct tttgtaatga
    16441 tcgcattttc atctgctgtt ccaccaacaa tatcttcttt cttggcaagt gttaatccta
    16501 attcttctgg atcgatcgta tattctttga tctcatctcc attgatctca cagacacttg
    16561 ttgtggatga gattgagatt tcatctaatc cgtcgtctcc atatacgatc agggctcttt
    16621 taactccaag attcttcata acatctgcca tcggacgaac cagctctttt tcataaacac
    16681 cgagaacgat gtagtttgtc attgctgggt ttgctaaagg tcctaagata ttaaatacag
    16741 atcttacccc aagctgtgct ctgactggtc cggcatattt catagatccg tgatgtgtct
    16801 gtgcaaataa gaaggctgct ccaactttat ccaaacattt cttgctttct tctggtgtta
    16861 atccgatttt tgctcctaat gcttctaaga catcggctgc accacttttt gaggatacac
    16921 ttctatttcc atgtttcgca acttttgctc ctgctgctgc aatgacaaat gcagatgttg
    16981 tggaaatatt aaagctgttt gcaaggtctc ctccagttcc tacgatatcg atcgcttctg
    17041 tttcttctgg cacgatcgct gcttttgctc tcatcacttt ggcaaatcct gtgatctctt
    17101 ctggtgtttc atgattcata cgaagacccg tcaggaaact tcccatctga gcatctgtcg
    17161 catttccaga catgatgcag tccatggcct tataagcttc ttcttctgtt aaatgatttc
    17221 cttccacgat ctttgttaaa aatggtttta atgtttcctg attgactgcg ctcatagttt
    17281 cctccttttt agaatctcca agtctgattc ctgcgatgtt tgttaaaaag ttttctaaga
    17341 tcctcattcc catttcagtt aagatcgatt ctggatggaa ctgaattcca tagactggat
    17401 attctttatg acatactgcc atgatctgtc ctttttcatc tcttccgata acttttaaat
    17461 cttctggaaa tgtctcatcg tcgatgatca gtgaatgata tcttgcggca tatattttat
    17521 cttctaatcc ttcaaataaa ggattcttgt tattgattgt gatctcactc atttttccat
    17581 gcatcaattc ttttgctgga acgacttttc cgccatatac ttctccgatc gcctgatgtc
    17641 ccagacagat tcccaagact gggatttctt ttccgaatgt tttaattgcc tcttttgaaa
    17701 tccctgcact gtctggatat ccaggtcctg gtgagataac aagtgcttct agattttgta
    17761 agtttctgat ctcatcaatc gttatttcat cgtttcttac aacctgaatc tgtggataca
    17821 aagatccaat gtactgatat acgttatagg taaatgaatc gtaattatct atcattaaaa
    17881 tcataacctt gctgcctcct tcactgcatt tacaacggct aatgctttat tctttgtctc
    17941 cataaattct ttctctggaa cagagtcatt gacgattcct gctcctgcct gcacatatcc
    18001 ttttccatct ttaaatacga cggtccggat tgcaatacag gtatcaatat ttccatcaaa
    18061 tccaagatat ccaacggttc caccgtaaac acctcgtttt ttattttcca gctcatcaat
    18121 gatctccatg gcccttactt ttggtgctcc ggataatgtt cctgccggaa gaacactcat
    18181 cagtgcgttg atcggatttt catcatctct taattctccc tgtacatcac tgactaaatg
    18241 tgtaactttg gaatattttt caacggtcat aaagtttttt acttctacag ttccaaactt
    18301 actgactttt ccgacatcat ttcttccaag atcaactaac atcgtatgtt ctgctctttc
    18361 ctttggatca ttgactaact gtcttaccaa catatcatct tcttcttttg tctttcctct
    18421 tggaaccgtt cctgcgatcg gtttggttgc tacaattcca tttaagacac tgactaactt
    18481 ctctggtgaa gctccaacca cctgatagtc aatgaaatca aagaagtaaa ggtatggaga
    18541 tgggtttgat gttcttaagc atcgatagac atcaaatgga tcaacatctg tctctacctc
    18601 aaatctctgg gataagacca cctgaaaaat gtcaccgttt ttgatatatt ctttggcttt
    18661 ctttacgttc gcttcgtatt gttcttttgt aagattcgat gttacttctg ccttcgcagg
    18721 tgtaaatctg tctttggaaa gtgataccgg acgttccatc ttgtgaagga tcaattctgc
    18781 tttatcttct aattcttcat atttttgttt gatattatca cctttgtgga tgttctggat
    18841 gatcacagct ttgtttgcta aatgatcgaa tgcgatgatc tcatcgtaca tgcataagtg
    18901 gcattctggc atcttcagat catcttctgg tacatttgtt aatttctttt ccacctgacg
    18961 gatcatgtca tatccaaaat atccgatcaa tcctccagtt aattttggat aatcttccat
    19021 aactggagat ttatatttct cgattagatc acttaggtaa ctcatcttga attcttcttc
    19081 ctgctttact ccatcgactt ctaattcttt tcctgaaatc ttgatctctg acttaggatt
    19141 gatcccgata aaggaatatc ttccccactg atctttattt tccacactct caagcatgaa
    19201 acatggcgtt ccttcttttc tcagtgcctg gaacatacgg atcggtgtca tataatctgc
    19261 cagtacttcg taaaatacag gtaccatctg atattctttt aatagttctt caactttgtt
    19321 taatgtcgga tataacatat ctgcctcctg atcaaaattt atcgccgtgg ttttattttt
    19381 catagtttgt atatctgtat actatgattt atagatagga ccgttttgca taagcagtac
    19441 attttctttt atttatgaaa taaaaaaggc ccatcgttcc tactactagg gacgatagac
    19501 cgtggtgcca cccatattca agaaatatct tctgatcttc tcttcttgtt aaaatatctt
    19561 agaaaatatc tctcctcact taagatactc cgctttgttc gataataaaa agcttttata
    19621 tctcgttccc gtaacgtggg ataacggcga cagttactaa gtttcataaa cagttctctg
    19681 tgctcctcac aaatccattc gcttatcaca tatctatgac cttccagcaa ccggtcactc
    19741 tctgtaagac tgtttgatga gctacttact tttgttcatc ggatttatca ctttaacttg
    19801 ttaaatattt gtttatatta tatgagtcga ttttttattt gtcaagaatt ttctgattat
    19861 aatttttgat ttgttgaaaa ttcgcatatt ttatttttct attttaacat tttataaatc
    19921 tcttctttat cagcttgtgg atgctctttg attgcttttt taattcttta ttctatgaaa
    19981 acttagcaat taatccctca tataccataa actgttccat agatgaaaat ttcacatgat
    20041 ctac
//
LOCUS       DAOMUE010000009        19522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_475_length_19522_cov_2.895361, whole genome shotgun sequence.
ACCESSION   DAOMUE010000009 DAOMUE010000000
VERSION     DAOMUE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 19522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19522
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_475_length_19522_cov_2.895361"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1262)
                     /locus_tag="PLC71_01095"
     CDS             complement(<1..>1262)
                     /locus_tag="PLC71_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRM01274.1"
                     /translation="DIRPMVQLDGGRFATSDLNDLYRRIINRNNRLKRLLELGAPDII
                     VRNEKRMLQEAVDALIDNGRRGRPVTGPGNRALKSLSDMLKGKQGRFRQNLLGKRVDY
                     SGRSVIVVGPDLKIYQCGLPKEMAIELFKPFVMKELVERKLAHNIKSAKKMVEKLQPE
                     VWDVLEDVIKEHPVMLNRAPTLHRLGIQAFEPILVEGKAIRLHPLVCTAYNADFDGDQ
                     MAVHLPLSVEAQAECRFLLLSPNNLLKPSDGGPVAVPSQDMVLGIYYLTLEKDGDKGE
                     GKYFKSENEAYLAYENKVITLHSKIKVRRTGVDKDGNPISQIVDCTLGRILFNEIILQ
                     DLGFVDRSKPENALAYEIDFHVGKKQLKQILDRCINIHGATKTAEVLDDIKAIGYKFS
                     TRGALTVSISDMTVPPEKPQILGDAQKQ"
     assembly_gap    1263..1272
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1273..1424
                     /locus_tag="PLC71_01100"
                     /pseudo
     CDS             1273..1424
                     /locus_tag="PLC71_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160427.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1406..2125)
                     /locus_tag="PLC71_01105"
                     /pseudo
     CDS             complement(1406..2125)
                     /locus_tag="PLC71_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
     gene            complement(2159..5956)
                     /locus_tag="PLC71_01110"
     CDS             complement(2159..5956)
                     /locus_tag="PLC71_01110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRM01275.1"
                     /translation="MEKNRIQPIKSGTGMRISYARQDDVLEVPNLIEIQKDSYDWFLR
                     EGLAEVFEDISPIEDYSDQLSLEFVDYQLCKDDVKYSIEECKERDATYAAPLKVKVRL
                     HNKETDEIKQHEIFMGDLPLMTATGTFIINGAERVIVSQLVRSPGIYYGIAHDKIGKE
                     LYSSTVIPNRGAWLEYETDSNDVFNVRVDRTRKVPITVLIRALGIGTNAEIKELFGEE
                     PKILASIEKDPSDNYEDGLLELYKKIRPGEPLSVDSAESLINSMFFDPRRYDLAKVGR
                     YKFNKKLALRNRISGFILAEDVLDPTTGEVLAEAGTNVDMKLADKIQNAAVASVMIQT
                     EEGATKVLSNMEVNLAEYVDFNPEEIGVHEDVFYPVLEKILEEGYEGEELKEALKKNI
                     HELIPKHITKEDILASINYNIHLEYGIGHDDDIDHLGNRRIRAVGELLQNQYRIGLSR
                     LERVVRERMTTQDIESISPQSLINIKPVTAAVKEFFGSSQLSQFMDHNNPLSELTHKR
                     RLSALGPGGLSRDRAGFEVRDVHYTHYGRMCPIETPEGPNIGLINSLATYARINEYGF
                     IEAPYRVLDKSDPENPVVTDKVVYLTADEEDNYVVAQANEPVDEDGHFVNTHVSGRFR
                     EDTSEFDKVNLDLMDVSPKMVFSVATSMIPFLENDDANRALMGSNMQRQAVPLLSTEA
                     PVVGTGMESKAAYDSGVCITARNGGVVERSTSTKIVVRNDDGQRDEYNVIKFARSNQG
                     NCMNQRPIVFVGDRVEKGDILADGASTSNGEMALGKNPLIGFMTWEGYNYEDAVLLSE
                     RLVQDDVYTSVHIEEYEAEARDTKLGQEEITRDLAGLSEDVLKDLDDNGIIRIGAEVR
                     AGDILVGKVTPKGETELTAEERLLHAIFGEKAREVRDTSLRVPHGAYGVVMDTKIFTR
                     ENGDELPPGVNKSVRVYIAQKRKISVGDKMAGRHGNKGVISRILPVEDMPYLPNGRPL
                     DIVLNPLGVPSRMNIGQVLEIHLSLAAKVLGFNVATPVFNGADENDIADTLRMANDYA
                     NTSWEEFDAKWRDKVNDDVMDYLYENRDHRKEWQGVPIDTTGKVRLRDGRTGEEFDSP
                     VTIGFMHYLKLHHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYG
                     AAYTLQEILTFKSDDVVGRVKAYEAIIKGDNIPEPGVPESFKVLLKEFQSLALDVKIL
                     DENAEEVELREDVDGGDANQDLAPMIEGDGVFNNYEESESELGALGYKHGDLSEGEDN
                     TIM"
     gene            6327..6608
                     /locus_tag="PLC71_01115"
     CDS             6327..6608
                     /locus_tag="PLC71_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01276.1"
                     /translation="MGITLKHNKLLLPAPCTAFIAGLIWDKVSSRVAVSSIFIGMICG
                     IIAYFMIPDDNINYFVGNVFSLCVPIVVIMIGSLFEKEHFDFEQLNYLF"
     gene            6812..7370
                     /locus_tag="PLC71_01120"
                     /pseudo
     CDS             6812..7370
                     /locus_tag="PLC71_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(7470..8123)
                     /locus_tag="PLC71_01125"
     CDS             complement(7470..8123)
                     /locus_tag="PLC71_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM01277.1"
                     /translation="MITAVIFDMDGVIADSEYFNVKAKHLILKRAGIEVDWHYHDKFL
                     GTTHEYMWTEMKKEFESLDKEVLYYIDQWVKTRKELIDQEGLKPMPGVVDLIRILKEK
                     GFHLAVASSSLKEDIMTNMNTFGITDCFEAFISGSECENGKPDPEIFQKAAEAIGQKA
                     ANCIVVEDSEAGVKAAKSAKMKCIGYAPEGAIKQDLHQADTVVKEFSDKLIEYIMEK"
     gene            complement(8195..>9423)
                     /locus_tag="PLC71_01130"
     CDS             complement(8195..>9423)
                     /locus_tag="PLC71_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRM01278.1"
                     /translation="LLTYLILTLTFGFSLMITEVALGRKAGTSAIRAFGHFSKKHTFI
                     GYLTTIIPFIIFPYYCVIGGWVTKYALISLQGGIHNAASDTFFTGFISKSAEPMICVL
                     VFLIATTVVVAGGVKGGVERVSTIMMPVLIVLLIGVSLFCITRPGAMAGVAYYLKPSL
                     KGFGPTTILAALGQLFYSMSLAMGIMITFGSYMPKKADLEKSVTQVEIFDTGVAFLAG
                     LMIVPAVFVFSNGDSSMLAKGPSLMFVMLPKVFDSMAFSSVIAAVFFILVLLAALTSS
                     ISLLETLVAVLMDKFHMKRGTACITMFIIALLLAVPSSLGFGAWSNITILGFDFLDFF
                     DFISNSVLMPIAAFLTCLFVGYIIKPKVIVDEVESSGKFKRKSLFLIMVKYIAPICIV
                     AILVFSVLEGLGFITV"
     assembly_gap    9424..9433
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9434..9556)
                     /locus_tag="PLC71_01135"
     CDS             complement(<9434..9556)
                     /locus_tag="PLC71_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01279.1"
                     /translation="MSNERSGFSSRLGFVLATAGSAVGLGNIWRFPYLAAKYGGG"
     gene            complement(9677..10978)
                     /gene="thiC"
                     /locus_tag="PLC71_01140"
     CDS             complement(9677..10978)
                     /gene="thiC"
                     /locus_tag="PLC71_01140"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRM01280.1"
                     /translation="MKYTTQMNAARQGIVTKEMEAVAAYEGIDVKDLMAEVAAGTIVI
                     PANKNHKCLKPFGIGNSLKTKINVNLGTSRDCLNLDVEMEKVNKAVEMGAEAIMDLSS
                     FGHTHVFRKKLVNECPAILGTVPIYDAIVYYNKALKDITSREWIDVFKMHAEDGVDFM
                     TIHCGINRNTAERFKAMKRKMNIVSRGGSLIFAWMEATGNENPFFEYYDEILDICNEY
                     DVTLSLGDACRPGCLADASDISQIEELVTLGELTARAWKKDVQVMIEGPGHMPMDQIQ
                     ANMKIQQTICHGAPFYVLGPLVTDIAPGYDHITSAIGGAIAAASGASFLCYVTPAEHL
                     RLPNVDDVKEGIMASKIAAHAADIAKGVKGAMDWDNQMAAARQKLDWEKQFELALDPE
                     KARRYRAESTPEREDTCTMCGKMCSVRNMNAVLAGEDVDVI"
     regulatory      complement(11114..11225)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(11316..12677)
                     /locus_tag="PLC71_01145"
     CDS             complement(11316..12677)
                     /locus_tag="PLC71_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRM01281.1"
                     /translation="MGKNKVEKVMKKLCVCTLSAVIGMLSIQIMPVSAASKTGMGKIT
                     NKINKAGYNLWGGYDSPEEAATYDIDFYDSGINAGVAVGGNISLKYNDFYDYGDNLKY
                     NWQIVNGNDHIAMEVSADQKTARITGRSIGDATVRLNIITDEGTEYQSIETKEMHIQI
                     SNPRLKNNKLATVLYNEGKVELEGNSTNGQERIIYRADNNNNFYVSDDGTFYGYAKQT
                     RKIYVYVDGICLEATVKCTDPQYRASCILKKGQKVSYKVSGASGYTPVTYKVGNTKYA
                     SVSSNGLTKGKKYGRTTLTISADNASVSFNIYILKSKVYKGVSKAQAICKTRPRYSQA
                     KRMRKGYVDCSSFVWKSYKPYGVNFGSKSYAPTAADIAKWCGKKKKLLKLSTYNGKSD
                     RLLPGDLIFYRKRSGKNGRYKNIDHIAMYVGNDTIVHADGSSVSYSYPWYRKVAAIAR
                     PVK"
     gene            complement(12720..13664)
                     /locus_tag="PLC71_01150"
     CDS             complement(12720..13664)
                     /locus_tag="PLC71_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRM01282.1"
                     /translation="MKLVIIEPLGVEEEKLMEIAHKALPDSVEIVSYQTKVTDTESLI
                     ERGKDADIIMVANLPLNAEVIKGCKNLKMLAVAFTGIDHIAMDACKEQGITVCNCAGY
                     SNAAVSDLVFGLLISLYRNIISCDQVVREEGTKDGLVGFELEGKKFGVIGTGAIGTRV
                     AKIAQAFGCEVYAYSRTVKDLPGIQFVGLEELLYNCDIISLHVPLNESTKGLIGKDEI
                     ALMKPDAVLINTARGPVVDSEALAEALNNGKIAGAAVDVFENEPPVSKDHPLLHAKNL
                     IATPHVAFATKEALVKRAIIDFDNVKCWMEGKPVNVMN"
     gene            complement(13873..14235)
                     /gene="rplL"
                     /locus_tag="PLC71_01155"
     CDS             complement(13873..14235)
                     /gene="rplL"
                     /locus_tag="PLC71_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRM01283.1"
                     /translation="MTTQEIIEVIKGLSVLELNDLVKACEEEFGVSAAAGVVVAAAGA
                     EGGAEEKDEFDVELTEVGGAKVKVIKAVREVTGLGLKEAKELVDGAPKVIKEGASKAE
                     AEDIKAKLEEQGAKVTLK"
     gene            complement(14278..14781)
                     /gene="rplJ"
                     /locus_tag="PLC71_01160"
     CDS             complement(14278..14781)
                     /gene="rplJ"
                     /locus_tag="PLC71_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRM01284.1"
                     /translation="MAKVELKQPVVNEIKELLTDAQSAVLVSYRGITVEQDTAMRKEL
                     READVEYKVYKNTMIRFAIQGTEFEALADDLKGPTAIAVSKTDATAPARILAKHAKNI
                     EVLEMKSGVVEGTFYDKDGIQVIASIPSRDELLAKFLGSIQSPITNFARVIKQIAEKD
                     GEGAAAE"
     misc_feature    complement(14823..14957)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(15104..15796)
                     /gene="rplA"
                     /locus_tag="PLC71_01165"
     CDS             complement(15104..15796)
                     /gene="rplA"
                     /locus_tag="PLC71_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRM01285.1"
                     /translation="MKKGKRYAELTKLVDRTNNYDPQEAVALVKKLANAKFDETIEAH
                     IRLGVDGRQADQQIRGAVVLPHGTGKEVKVLVFAKDAKAEEAKAAGAEFVGAEELIPK
                     IQKENWYDFDVVVATPDMMGVVGRLGRDLGPKGLMPNPKAGTVTMDVANAIKEIKAGK
                     IEYRLDKSNIIHVPVGKASFTEEQLADNFQTLIDAIVKAKPAAAKGQYFRNVTITSTM
                     GPGVKIDTTKFA"
     gene            complement(15860..16285)
                     /gene="rplK"
                     /locus_tag="PLC71_01170"
     CDS             complement(15860..16285)
                     /gene="rplK"
                     /locus_tag="PLC71_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRM01286.1"
                     /translation="MAKKVEGYIKLQIPAGKATPAPPVGPALGQHGVNIVQFTKEFNA
                     RTADQGDMIIPVVITVYQDRSFSFITKTPPAAVLLKKACKIQSGSGEPNRTKVATISK
                     ADLQQIAETKMKDLNAASIEAAMSMIAGTARSMGITVEE"
     gene            complement(16371..16916)
                     /gene="nusG"
                     /locus_tag="PLC71_01175"
     CDS             complement(16371..16916)
                     /gene="nusG"
                     /locus_tag="PLC71_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRM01287.1"
                     /translation="MAEANWYVVHTYSGYENKVKVDIEKTIENRKLQDEILEVRVPLE
                     DVVEMRNGVKKQVSRKMFPGYVLIHMIMNDDTWYVVRNTRGVTGFVGPGSKPVPLTPA
                     EMKPLGIQIGGEQQETPAREKIELDIEVGDMIVVTSGAWEGTTSAVTAINESKQTVTI
                     EVEMFGRATSVEIDFLDIKKA"
     gene            complement(16932..17129)
                     /gene="secE"
                     /locus_tag="PLC71_01180"
     CDS             complement(16932..17129)
                     /gene="secE"
                     /locus_tag="PLC71_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRM01288.1"
                     /translation="MEKTNKAKKTSWFKELKAEFNRIIWPTKERIAKETAVVVICAII
                     IGVIVAVLDVGIQYGIQALVG"
     gene            complement(17169..17333)
                     /gene="rpmG"
                     /locus_tag="PLC71_01185"
     CDS             complement(17169..17333)
                     /gene="rpmG"
                     /locus_tag="PLC71_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRM01289.1"
                     /translation="MEVEVVRTRVTLECTECKQRNYNMTKDKKTHPERMETKKYCKFC
                     KTHTLHKETK"
     gene            complement(17654..18859)
                     /locus_tag="PLC71_01190"
     CDS             complement(17654..18859)
                     /locus_tag="PLC71_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01290.1"
                     /translation="MVKNFLKRTLCIVLSFAMMTGTVMVGAKTKTENIHSKLPAITAS
                     QRVRAKQAKASAAAVQLNATNVYTLSDFDTLSNKWQANQEMMLYQGYSDANEYVKVGT
                     IRVGIDGTVVIPYATKVAGVDGGRLKLCYNGNPAMTLSQTNNGKMIWAGAVKAGTYAL
                     YLETACENANTPVVAAFGAAVISSQNNRTFGGNNVIASASTGAATYQQFKVSKRGVAA
                     IAVYKENSAGNVSSVTYSVQKLSGKTWKTVATGLSGAAKNNYVVTTGLAAGTYRVVAN
                     AASGEILYFINANQKASDSYGTSKKKAKEIKRKKSKKQVFLSNESTSKTHWYKIKVKK
                     TGMTYIDITGLGNKSKISVTVTGAARLKNKTISKGFRIYGKAKKGTYYIKIKKGSKGT
                     SGYQVKYTK"
     gene            complement(18871..>19522)
                     /locus_tag="PLC71_01195"
     CDS             complement(18871..>19522)
                     /locus_tag="PLC71_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01291.1"
                     /translation="THLSTGKKYNWSVDSVYVNTTPADVAKNNFGITAAYANIKKVAF
                     KVNKPEMATGVQLEVYNAKNKRVLSKTSTSMGYESMNVSKDMAYKYRARYYYTNRSNN
                     QTYYGRWSNYRYFAMPSISGKTTNKKKGIKVVLKKGRGIKQYTVSISKKSKSGFKKVK
                     TVKVSKKKSYSFQITKNGKKKLKKGTYYVQVAPKVKFGNKTYSSDVSTVASAYVYK"
BASE COUNT         5160 a         4280 c         3220 g         6842 t
ORIGIN      
        1 ctgtttctgt gcatctccta agatctgagg tttctcaggt ggcactgtca tatcggaaat
       61 agatactgtt aaggctcctc ttgtagagaa tttgtatccg attgctttaa tatcatctaa
      121 gacttctgca gtctttgttg ctccatgaat attgatacat ctgtcaagga tctgttttaa
      181 ctgtttcttt cctacatgga aatcaatctc atatgctaag gcattttctg gtttgcttct
      241 gtctacaaat cctaaatcct gtaagatgat ctcattaaac agaatacgtc caagtgtaca
      301 atctacgatc tgggaaatag gatttccatc tttgtccaca cctgtacgtc ttactttgat
      361 cttagaatga agtgtgatta ccttattttc ataagctaag tatgcttcgt tctcagattt
      421 gaagtattta ccttcacctt tgtctccatc tttctctaat gtcagatagt agataccaag
      481 taccatatcc tgagaaggaa cagctactgg accaccatca gatggtttta acaggttgtt
      541 tggtgataac agaaggaatc tacactctgc ctgtgcttcc acagataatg gaagatggac
      601 agccatctgg tcaccgtcga agtcggcatt atacgctgta cataccaatg ggtgcagacg
      661 aatcgcttta ccctctacca ggataggctc gaatgcctga attcccagtc tatgcagtgt
      721 cggtgcacgg tttaacataa caggatgttc tttaatgacg tcttctaaga catcccatac
      781 ttctggctga agtttttcaa ccattttctt agctgattta atgttgtgag ctaatttgcg
      841 ctctactaat tctttcataa cgaatggctt gaataattcg atcgccattt cttttggaag
      901 accacactga taaatcttta agtctggtcc tactacgata acggaacgtc cagaatagtc
      961 tacacgtttt cctaacaggt tctgacggaa acgtccctgt ttacctttta acatatctga
     1021 taatgatttt aatgcacggt ttcctggtcc tgtgactgga cgtccacgtc taccattatc
     1081 gatcagggca tcaactgctt cctgtaacat tctcttttcg tttcttacga tgatatctgg
     1141 agctccaagc tctaataatc tcttcagacg gttatttctg ttaatgatac gtctgtaaag
     1201 atcatttaag tcagatgtgg caaaacgtcc accatctaac tgcaccattg gacggatatc
     1261 tgnnnnnnnn nngtcccatc ggggtttatg agatttttgt aagggaagca gagatcggac
     1321 agctgtataa gttttttatt acaggagctc acggggagaa gctttacaaa gcagatccct
     1381 atgctaatga ggctgagaag cgccccggaa ctgcatccag gatcatccat tctggtttgt
     1441 tgtctgagtt taaaaatgcc tctacaactt ccagtctctt aacgattctg gctctcttct
     1501 gacctgtagc agttttgaat tcatcctgta aggattttgc ttctgcctca agatcaatgg
     1561 cctgaagcag ttctttcact gcttcagcac ccattcctac gcggaatgta tctccgtatt
     1621 tttcatacgc ttctctatat tctttttctg ttaatacttg tttatatgca agatctgtat
     1681 ctccagcatc taaaacgata tagcatgcga aatataaaac cttttccagt gttcttggtg
     1741 aaatatcaag aatcagaccc atacggctag gaattccttt aaaataccag atatgagata
     1801 ctggagctgc taattcgata tgtcccattc tttcacgacg gacacttgct tttgtcactt
     1861 caacaccgca tcggtcgcag acaacacctt tatagcgaac ttttttgtac tttccgcagt
     1921 gacactccca gtcctttgta ggcccgaaga tcttctcaca gaaaagcccg tctttttcag
     1981 gttttaatgt tcgatagttg atcgtctcag gtttctttac ttctcctcta gaccactcac
     2041 ggatcttttc tggggaagcc aacccgatct taattgcatc aaatgaaatt ttattgcttt
     2101 gatattcatt gcttgattcc atcatttaag gctgctcctt cctaaattgc tcgccaatct
     2161 acatgattgt gttatcttca ccttctgata agtctccgtg tttatatcct aaagcaccga
     2221 gttcagattc gctttcttca taattgttga agacaccgtc tccttcgatc attggtgcaa
     2281 gatcctgatt tgcatctcca ccatcaacat cttctcttaa ttcaacttct tcagcgttct
     2341 cgtcaaggat cttaacatct agggccagtg actggaattc ttttaataat actttgaaag
     2401 attctggaac acctggttca gggatgttat cacctttgat gatcgcttcg tatgctttca
     2461 cacgacctac aacgtcatcg gacttgaatg tcaggatctc ctgaagtgtg tatgctgcac
     2521 cataagcttc cagtgcccat acctccatct ctccgaaacg ctgtccaccg aactgagctt
     2581 taccacccag tggctgctga gttactaatg agtaaggacc agtagaacgt gcatggatct
     2641 tgtcatctac cagatgatgc agtttcaggt aatgcatgaa tccgattgtt acagggctgt
     2701 caaattcttc tcctgtacga ccgtctctta atcttacttt accagttgta tcaattggta
     2761 caccctgcca ttcttttcta tggtctctgt tttcatataa gtaatccatt acatcatcgt
     2821 taactttatc acgccatttc gcgtcaaatt cttcccatga tgtattggca tagtcatttg
     2881 ccattcttaa tgtatctgcg atatcgttct cgtctgcacc attgaatact ggtgtagcaa
     2941 cattaaatcc aagaactttt gctgctaagg acagatggat ctctaacacc tgtccgatat
     3001 tcatacgtga aggcacacct aatgggttca gtacgatatc taatggacgt ccgtttggta
     3061 agtatggcat atcttctact ggcagaatac gggaaattac acccttgtta ccatgtcgac
     3121 cagccatctt atctcctaca gaaatctttc tcttctgagc aatgtataca cgaacgctct
     3181 tgtttactcc aggaggaagt tcgtctccat tctctcttgt gaagatcttt gtatccataa
     3241 caactccgta agcaccatgt ggcactctta aagatgtatc acggacttct cttgctttct
     3301 caccgaagat cgcatgtaac agtctctctt cagctgttaa ttctgtttct cccttaggag
     3361 taacttttcc taccaggatg tcaccggcac gtacttctgc accgatacgg atgataccgt
     3421 tatcatctaa gtcttttaag acatcttcac ttaatcctgc aagatctctt gtgatttctt
     3481 cctgaccaag cttagtatct ctggcttctg cttcatattc ttcgatatga acagatgtgt
     3541 aaacgtcatc ctgtaccaga cgctcactta acagtaccgc atcctcgtag ttatatcctt
     3601 cccatgtcat gaaaccgatc aatgggttct taccaagagc catctctccg ttgcttgttg
     3661 aagcaccgtc tgctaagatg tctccctttt ctactctgtc tcctacaaat acgatcggac
     3721 gctgattcat acagttaccc tggttgcttc gtgcaaactt gataacattg tactcatctc
     3781 tctgaccgtc atcattacga actacgatct ttgtacttgt agatctttct acgactccgc
     3841 catttcttgc tgtgatgcag actccagagt catatgcagc cttggattcc atacctgttc
     3901 caacaacagg agcttctgta gaaagaagtg gtactgcctg acgctgcatg ttggatccca
     3961 tcagcgcacg gttggcatca tcgttctcca ggaaaggaat catggatgta gctactgaga
     4021 ataccatctt tggagatacg tccattaagt caaggtttac tttatcaaat tctgatgtat
     4081 cctcacggaa acgtccggat acatgagtat ttacgaaatg tccatcttcg tcaacaggtt
     4141 cgttcgcctg tgctactaca taattatctt cttcatcagc tgttaaataa acaactttgt
     4201 ctgtaacaac tggattctca ggatctgatt tatccagtac acgatatgga gcttcgataa
     4261 atccatactc gttaattctc gcataagtag caagagagtt gatcagaccg atgttaggtc
     4321 cttcaggtgt ctcgatcgga cacattcttc cgtaatgtgt ataatgtacg tcacggactt
     4381 cgaatccggc acgatcacga gacagaccac ctggtcctaa tgcagataaa cgtctcttat
     4441 gtgttaactc agacagtggg ttgttgtggt ccatgaactg agataactga gaacttccaa
     4501 agaactcttt cactgctgct gttactggtt tgatattgat caatgactgt ggtgagatag
     4561 attcaatatc ctgagttgtc attctttcac gaacaactct ttccaatctt gaaagaccga
     4621 tacgatactg gttctgaagc agttcaccta cagcacggat acgacggttt cctaagtggt
     4681 caatatcatc atcatgtcca attccatact ctaaatggat attatagttg atggatgcta
     4741 agatatcttc ttttgtaata tgttttggaa tcaattcatg aatattcttc ttcagagctt
     4801 cttttaattc ttctccttca tatccttctt ctaagatctt ctctaataca ggatagaaaa
     4861 catcttcatg aacaccgatc tcttctggat tgaaatctac atattcagca agatttactt
     4921 ccatgttgga aagtactttt gtagcacctt cttctgtctg gatcataaca cttgctacgg
     4981 ctgcgttctg aatcttatct gcaagtttca tgtccacatt cgttccagct tctgctaata
     5041 cttccccagt tgttggatct aaaacatctt ctgctaagat aaatccactg atacgatttc
     5101 ttaatgctaa ttttttattg aatttgtaac gtccaacttt cgcaagatca tatcttcttg
     5161 gatcgaagaa catgctgttg atcaagcttt ccgcactatc tacagataat ggttctcccg
     5221 gacggatctt cttatataat tctaacagac catcttcata gttatcagat gggtcttttt
     5281 caatacttgc aaggatcttt ggttcttcac caaataattc tttgatctct gcgtttgttc
     5341 cgatacctaa tgcacggatc aaaactgtga taggcacttt tcttgtacgg tctacacgta
     5401 cattgaatac atcattggaa tctgtttcat attcaagcca agcaccacgg tttggaataa
     5461 ctgtggaaga gtataactct ttaccaatct tgtcatgagc aattccataa taaattccag
     5521 gggaacgcac taactgactg acgataacac gttcagctcc gttgatgata aatgttcctg
     5581 ttgctgtcat taaaggaaga tcgcccataa aaatctcatg ctgcttgatt tcatcagtct
     5641 ctttattatg aagtcttact ttcactttta aaggtgcagc gtatgttgca tctctttctt
     5701 tacattcttc aattgaatat ttgacgtcat ctttacataa ttgatagtca acaaattcca
     5761 agcttaactg atcgctgtaa tcttcgattg gggagatatc ttcaaatacc tctgctaatc
     5821 cttctcttaa aaaccaatca taggaatcct tttgaatttc gatcagattt ggtacttcaa
     5881 ggacgtcatc ctgtctggca tagctgattc gcatccctgt acctgatttt attggctgta
     5941 ttctgttctt ttccatggac gtttttcacc tcttgatcta aacctggttt atatactaca
     6001 tcaactgtaa gaaaccctct atcttactat atctttctta caatcttatt cttaatttta
     6061 catagagtgt gggcagacaa acccacatta agacattttc ttatattacc atatagaatt
     6121 tatcatgtca ataactattt ttaaatacat gaaaaaagca cccgaaaatg tttctcatct
     6181 gcctttttca ttaaattctt cacttctttt cactcttatc ttgtaataaa aagattttgt
     6241 atgttacact agtcataaac aaaggcgttg taaacagata tatgtttata gcgccttttg
     6301 catttattaa gaaaggaaat cttaacgtgg gaatcacttt aaaacacaac aaactacttt
     6361 tgcccgcccc atgtactgca tttattgctg gattgatctg ggataaagta tcctcaagag
     6421 ttgccgtttc atctatcttt atcggtatga tctgtggaat cattgcttac tttatgattc
     6481 ctgatgataa tatcaactat tttgtaggta atgtattttc tctttgtgtt ccgatcgttg
     6541 ttatcatgat cggctctcta ttcgagaaag aacattttga ttttgaacaa ttaaattatt
     6601 tattctaatt gtctatgtat ggtttgccat tggaattgca gtgatcagtg tatttgcaat
     6661 tcgagaattg tttagctggt tcaaggttcc aaacgagcat atatcacaaa gcattcagaa
     6721 aggaccgaga aaaaattatg aaatgaaatg taatgttgtt tgtatacaaa cttatccaaa
     6781 aatgaatcag aaagaggaaa atctttttca tatggaaacg cttcttcgca aagctatgga
     6841 atctcatcca gatactcaac tcatcgtttt ccctgagctt gctgttactg gttatcaatg
     6901 tggtgaaaat tttaaagatt tggcagaaac tgccatgatt gactctacat ccataaaaaa
     6961 gatgagtgcc ttagctaagg aatttcatgt tcatatcgtc tatggaatgg ctgaaaaaag
     7021 aagaagatat cctttataat tctcagtttt tccttgatga cactggtgtt cttcttggaa
     7081 cttatcgcaa agttcattta tttgattccg agaaaaatta ttttacacct ggggatcaat
     7141 ttaaagtatt tgatacaaag atcggtcgta tcggcttgtt tatctgttat gacgcattct
     7201 ttccagaaac tgctagaagt cttgatccat taggaaggat tctcgcatcg atcaatgatg
     7261 aaaaagatgg aattatccat gcatctctgg attatgatat agcaaaacaa atgaagcatg
     7321 actactatac aatgttgacg gaacgtcgtc cagatgttta tattgcatga atgaatattg
     7381 tgtcctatct tctcttttta cgagaaagtt tttataaaaa aatagacagt gctctttttc
     7441 ataagaacgc tgtctatttt ttattttact tatttttcca taatatattc tataagttta
     7501 tctgaaaatt ccttcaccac tgtatctgcc tgatggagat cttgtttgat cgctccttct
     7561 ggtgcatatc caatgcattt cattttggca gatttggctg cctttactcc tgcttcggaa
     7621 tcttcgacca cgatacagtt tgccgccttc tgtccaattg cctcagccgc cttttgaaag
     7681 atttctggat ccggctttcc gttttcacat tcggaaccgc ttataaatgc ttcaaagcag
     7741 tctgtaattc caaacgtatt catatttgtc atgatatctt ctttcaatga agacgatgct
     7801 actgccagat gaaatccttt ttcctttaat atacggatca gatcaacaac ccccggcatc
     7861 ggctttagtc cttcctggtc gatcaactct tttctggttt tgacccactg atctatataa
     7921 tatagaactt ctttatcaag actttcaaat tctttcttca tctctgtcca catatattca
     7981 tgtgttgtac ccaagaactt atcatggtaa tgccagtcaa cttcaatacc tgctcgtttt
     8041 aagatgagat gttttgcttt gacattaaaa tattctgaat cagcgatcac accgtccata
     8101 tcaaaaatca ctgctgtaat catactttcc tcctaataaa acagaaacgc acaaaatgtt
     8161 aagtagtcat ctgtgcgttt ctatatcttc tgtattaaac tgtaataaat cctaatcctt
     8221 caagtactga gaacactaag atcgcaacaa tgcagattgg tgcaatatac ttaaccataa
     8281 taaggaatag tgattttctc ttaaatttcc cagagctttc aacttcatca acaattactt
     8341 ttggcttgat gatataaccg acaaataaac atgttaagaa tgctgcgatc ggcattaata
     8401 cactgttact gatgaaatca aagaaatcaa ggaagtcaaa tcctaaaatc gtaatattgc
     8461 tccatgctcc aaatcctaaa gatgatggaa ctgctagtaa cagagcaatg ataaacattg
     8521 taatacatgc agttcctcgt ttcatatgga acttatccat cagaactgct acgagtgtct
     8581 caagcagcga aatggaagag gtaagtgctg caagtaacac taaaataaag aatactgctg
     8641 caataacact tgaaaaagcc atactgtcaa atacttttgg aagcatcaca aacatcaaag
     8701 atgggccttt tgcaagcatg gatgaatctc cgtttgagaa tacaaacaca gctggtacga
     8761 tcataagacc tgcaaggaaa gcaacccctg tatcaaaaat ctcaacctgt gtgactgatt
     8821 tctcaaggtc agctttcttt ggcatatatg atccgaatgt gatcataata cccatagcaa
     8881 gggacatcga ataaaataac tgtccaagtg cagctaagat cgttgtcgga ccaaatcctt
     8941 ttaaacttgg ttttaaataa tatgcaactc cagccatcgc ccctggtctt gtgatacaga
     9001 ataaagaaac tccgatcaac aagacgatca aaactggcat catgatcgtg ctgacacgtt
     9061 ctactccgcc tttgactcct cctgcaacaa ccactgttgt agcgatcagg aaaacaagaa
     9121 cacagatcat aggttctgct gatttgctga taaaccctgt aaagaatgta tcagaggcgg
     9181 cattatgaat ccctccctga agcgatatca gtgcatattt tgtaacccat ccaccgataa
     9241 cacagtagta cgggaagata ataaacggaa tgatcgttgt aagatatccg ataaatgtat
     9301 gcttcttgct aaagtgtcca aatgcacgga tcgcactggt tcctgccttt cttccaaggg
     9361 caacttctgt gatcatcagt gaaaatccaa atgttaatgt aaggatcaga taagttaata
     9421 aaannnnnnn nnntcctcca ccatatttag cagcgaggta tggaaatctc cagatgtttc
     9481 caagacctac ggcagaccca gccgttgcaa gaacgaatcc taaacggctc gagaatccac
     9541 ttctttcatt actcataata ttaagtacct tctttcatat atattcagat acgtaaggat
     9601 atgtatctgg ctctgccgga gatcttagat atgaatcctg catggcagga ttcccagcct
     9661 ggatgctgaa ttctatttag atgacgtcca catcttctcc ggcaaggacg gcattcatat
     9721 tacgtacaga acacatcttt ccacacattg tacatgtatc ttctctctct ggtgttgatt
     9781 ctgcacggta tcttcttgcc ttttcaggat caagagcaag ttcgaactgt ttctcccagt
     9841 caagtttctg tctcgcagct gccatctggt tatcccaatc catagcgcct tttacacctt
     9901 tggcaatatc tgctgcatgt gctgcgatct tggaagccat gattccttct ttcacatcat
     9961 cgacatttgg aagacgcaga tgttctgctg gtgttacata acataagaag cttgctccag
    10021 aagctgcagc gattgctcca ccaattgcag atgtgatatg gtcatatcca ggtgcaatat
    10081 ctgttaccag aggtccaaga acataaaatg gtgctccatg acagatcgtc tgctggatct
    10141 tcatattcgc ctgaatctga tccattggca tatgtccagg tccttcgatc ataacctgta
    10201 catccttctt ccatgcacgt gctgttaatt ctcctaatgt tactaattct tcgatctgtg
    10261 aaatgtcact tgcatcagca aggcatcctg gacgacatgc atcgccaagg cttagcgtta
    10321 catcatattc gttacagata tcaaggattt catcatagta ttcgaagaat ggattctcat
    10381 tgcctgttgc ttccatccat gcaaagatca gagaaccacc acgagacaca atgttcatct
    10441 tacgtttcat tgccttaaaa cgttccgctg tgtttcggtt gatcccacaa tggatcgtca
    10501 taaaatctac accatcttct gcatgcatct taaaaacatc gatccattca cgagatgtga
    10561 tatcctttaa tgctttattg taataaacaa tcgcatcgta aataggtacg gtaccaagga
    10621 ttgctggaca ttcattgaca agtttcttac gaaatacatg agtatgtcca aaagaagaaa
    10681 gatccatgat agcttctgca cccatctcta cggctttatt aactttctcc atctcaacat
    10741 ctaagtttag acagtctctg gatgttccaa ggtttacatt gatctttgtc ttcaaagaat
    10801 tcccgattcc aaatggcttt aaacacttgt ggttcttgtt tgcagggatg acaatagttc
    10861 cagctgcaac ttctgccata aggtctttca cgtcaattcc ttcataggca gcaactgctt
    10921 ccatctcttt cgttacaatt ccctgtcttg ctgcgttcat ctgtgttgtg tacttcatga
    10981 ttgaaaacct ccaaaatata agattttggg agactcttat tcggttttgt ttattttatc
    11041 atcattctgt cttgttgaga taaaaaaaga caaactttaa aaagcctgtg atgctgcttt
    11101 acagttcgtc ttgtattcat agcatccaaa tccctacgac ggcattatcc gtatcaggtc
    11161 catggtcgaa actctcgttt cctctcagct aaggtatcac cttttaagct cccatttcag
    11221 ttacatcaat attgtaccat cctaaaaggc agatagcaag aattttgtat ccttgttatc
    11281 tgccttttgt acttctttat ctttatattt ttcttttatt ttactggtct ggcgattgct
    11341 gcaactttgc ggtaccatgg atagctgtaa gacacactgc ttccatctgc atgtacgatc
    11401 gtgtcatttc caacatacat ggcgatatgg tcgatatttt tatatcttcc attctttcca
    11461 cttcgtttac ggtaaaagat tagatctccc ggaagtaatc tgtctgattt tccgttatat
    11521 gtagaaagtt ttaataattt cttctttttt ccacaccatt tagcaatatc tgcagctgtt
    11581 ggtgcatagc tcttacttcc aaaattcaca ccatatggtt tataagattt ccacacaaaa
    11641 gaactgcagt ctacataacc tttccgcatt cgttttgcct gactgtatct tggtcttgtc
    11701 ttacagatcg cttgagcttt actgactcct ttgtatactt tggattttaa gatataaata
    11761 ttaaaagata cactcgcatt atcagcactg atcgttaagg ttgtccttcc gtattttttt
    11821 ccttttgtta atccatttga tgatacagat gcatattttg tatttccaac tttatatgta
    11881 actggtgtat atccgctggc tcctgatact ttgtaagata ctttttgccc ttttttcaga
    11941 atacaactgg ctctatattg tggatctgtg cactttacag ttgcttccag acaaattcca
    12001 tctacatata cgtatatctt tctggtttgt tttgcgtatc cgtaaaatgt cccatcatcc
    12061 gatacataaa aattattgtt attatctgct cgatagataa tgcgttcttg tccattcgtc
    12121 gagttgcctt ctaattcaac tttcccttcg ttataaagaa ccgttgcaag tttgttattc
    12181 tttaatctcg gattggaaat ctggatatgc atttcttttg tttcaatact ttgatattct
    12241 gttccttcat ctgttatgat atttaatcgg actgttgcat ctcctatgga tcttcctgta
    12301 atccttgcag tcttctgatc tgctgatacc tccatcgcaa tatgatcatt tccattgacg
    12361 atctgccagt tatatttcag attatctcca taatcataaa aatcgttata ttttaatgaa
    12421 atatttccgc caacagcaac acctgcattg atgccagaat catagaaatc aatatcatat
    12481 gttgctgctt cttccggtga atcgtatccg ccccataaat tatatcctgc tttgtttatc
    12541 ttattcgtta tctttcccat gccagtctta cttgctgctg atacgggcat gatctgaatc
    12601 gataacattc ctataacagc tgaaagcgta catacgcaca gctttttcat cactttttcc
    12661 actttatttt tccccattta tcttgtttct cttaatgtat attcgactat ttataaaatt
    12721 taattcatta cattgactgg ttttccttcc atccaacatt tcacattatc aaaatcaatg
    12781 atcgcacgtt ttaccaatgc ttcttttgtt gcaaatgcaa cgtgtggtgt cgcgatcaga
    12841 ttctttgcat gtaagagtgg atgatctttt gataccggcg gttcattctc aaagacatca
    12901 acggctgctc ctgcaatctt accgttattg agtgcttcag ctaatgcttc actatcaaca
    12961 actggacctc ttgccgtgtt gatcaaaact gcatctggtt tcatcaatgc gatttcatct
    13021 ttaccgatca aaccttttgt tgattcattt aaaggtacat gcaatgaaat gatatcacaa
    13081 ttatataaaa gttcttctaa ccctacaaat tgaattcctg gaagatcttt tactgtcctt
    13141 gaatatgcgt aaacttcaca tccaaatgcc tgggcgatct tcgcaactct ggttccgatc
    13201 gctccggttc cgatcacacc gaatttcttt ccttccagtt caaatccaac cagaccatcc
    13261 ttggttcctt cttctcggac tacctgatcg caggaaatga tatttcgata cagtgagatc
    13321 aaaagtccaa agacaagatc tgaaactgca gcatttgaat agcctgcaca gttgcatact
    13381 gtaatcccct gttctttgca tgcatccata gcaatatgat cgatccctgt aaatgctacc
    13441 gccagcatct taaggttttt gcagcctttg atgacttcag cattcaatgg caggtttgca
    13501 accataataa tgtccgcatc ctttccacgt tcgatcaggc tttctgtgtc agttactttg
    13561 gtctggtaag atacgatctc gacagaatct ggcaatgctt tatgtgcgat ttccatcaat
    13621 ttctcttctt ccacacctag tggttcaata attactaatt tcataattgt cgaatactcc
    13681 tttcgtatga ttttactctc ctattctatc atcaaatata cgattataaa agcttagaaa
    13741 tattttattt catagaggat tccatcgaag tttcttatga tccatataaa gtaaaaaaac
    13801 tgccacacta gtttccacca atgtgacagt ttcatatact ttatagaaaa tctactggag
    13861 taaatctctt aattatttaa gagttacttt agctccctgt tcttctaatt tagctttgat
    13921 gtcttcagct tctgctttag atgcaccttc tttaattact ttaggagctc catcaactaa
    13981 ttctttagct tcttttaatc ctaatcctgt tacttcacga acagctttga tgactttaac
    14041 tttagctcca ccaacttctg ttaattctac gtcgaattcg tctttttctt ctgctccacc
    14101 ttcagctcca gctgctgcta ctacaacacc tgctgctgca gatactccaa attcttcttc
    14161 acaagctttt actaaatcgt ttaattctaa tactgataaa cctttgataa cttcaataat
    14221 ttcctgagtt gtcattataa tttcctccta atattttaaa tttactgata actgatctta
    14281 ctcagctgca gccccttctc cgtctttttc agcgatctgc ttaataacac gagcaaagtt
    14341 tgtaattggg gactgaatgc ttccaaggaa tttcgcaagt aattcgtctc ttgatgggat
    14401 gcttgcgata acctggatac catctttgtc atagaatgtt ccttctacaa caccagattt
    14461 catttctaat acttcgatgt tcttagcgtg ttttgcaagg attcttgcag gagctgtagc
    14521 atctgtttta gaaacagcga ttgctgtagg tccttttaaa tcatcagcta atgcttcaaa
    14581 ttctgttccc tggattgcaa aacgaatcat tgtattctta tatactttgt attctacatc
    14641 agcttctctt aattctttac gcattgctgt atcctgttct acagtgattc cgcggtaaga
    14701 aactaatact gctgactgag catctgtaag taattcttta atttcgttta ctactggttg
    14761 ttttaactca acttttgcca ttgaaatggt gcacctcctt gatttatttt gtgtaccacc
    14821 gcacatataa aaagaaaact ctatatccgc acagacatag agtgttaatt agcatattca
    14881 ctaatactct cctcggcagg ttgttctacg ttgcgccttg cggcacctgc tgtcttcggc
    14941 agtaatactt tacatataat acacgacatt tatatatatg tcaaatgttt ttttactttt
    15001 tttgaaatgt ttttattttt atgcaattcc catatggcaa cataggattt tgcataaatc
    15061 tggggtcaga ctacatgaga ctgaccccgc ttaaagttaa atattaagca aattttgttg
    15121 tatcaatttt cactccaggt cccattgtag aagtaattgt tacgtttcta aaatactgac
    15181 cttttgctgc tgcaggtttt gcttttacga ttgcatcgat taacgtctgg aagttgtccg
    15241 ctaactgttc ttctgtgaaa gaagcttttc caactggtac atgaatgatg ttggatttat
    15301 ctaatctata ttcgatctta cctgctttga tttctttaat ggcattcgca acgtccattg
    15361 ttacagttcc ggctttaggg ttaggcatta aacctttagg tcctaagtca cgtcctaaac
    15421 gacctactac acccatcatg tctggagtag ctactactac gtcgaagtca taccagtttt
    15481 ctttctggat cttaggaatt aattcttctg ctcctacgaa ttctgctcct gctgctttcg
    15541 cttcttcagc ttttgcatct ttagcgaata caagaacttt aacttcttta ccagttccgt
    15601 gtggaagtac aactgctcca cggatctgct ggtctgcctg acgtccatct acaccaagac
    15661 ggatatgtgc ttcgattgtt tcgtcaaatt tagcgtttgc taattttttt actaaagcaa
    15721 ctgcttcctg tggatcataa ttgtttgttc tgtccactaa ttttgtaagc tctgcgtatc
    15781 tcttaccttt tttcattcta aaatacctcc tgtggtaata tcgggagttc accctcccac
    15841 tttcgtttca aatttgtgac tactcttcta ctgtgattcc catacttctt gcagtaccgg
    15901 cgatcatgct cattgctgct tcgatagaag ctgcgtttaa gtctttcatc tttgtttcag
    15961 cgatctgctg aagatctgct ttagaaatag tagctacttt agttctgtta ggttctcctg
    16021 atccagactg gattttgcaa gctttcttta ataaaactgc tgctggtgga gtctttgtga
    16081 tgaagctgaa gcttctatcc tgatatacag tgattactac agggatgatc atgtctccct
    16141 gatcagctgt tcttgcgtta aattcttttg taaactgtac aatgtttact ccgtgctgtc
    16201 caagtgctgg tccaacgggt ggtgctggtg ttgcctttcc tgctggaatc tgaagtttga
    16261 tataaccttc tactttcttt gccataatcg ttaacctcct gattattgtg gtaattacgg
    16321 gatagccctc ccactttgtt tcaaataaga ttctctattc ttattcttcg ttacgctttc
    16381 ttaatatcta agaaatcaat ttctactgaa gttgctcttc cgaacatctc gacttcaata
    16441 gttactgtct gtttgctttc attgattgca gtaaccgcac ttgttgttcc ttcccaagca
    16501 ccggaagtca caacgatcat atcaccaact tcgatatcaa gttcaatctt ctctcttgct
    16561 ggtgtttctt gctgttctcc tccaatctgg attcctaatg gtttcatctc tgcaggagtc
    16621 agcggtactg gttttgatcc aggtcctacg aatcctgtaa cacctcttgt gttacgaacc
    16681 acataccatg tgtcgtcgtt catgatcata tggatcagaa cataacctgg gaacattttt
    16741 cttgaaactt gtttcttcac accgtttctc atctctacca cgtcttcaag tggtacgcgg
    16801 acttccaaga tctcatcctg aagcttacga ttctcgatgg ttttctcaat atccactttt
    16861 actttattct cataacctga gtaggtgtga acaacatacc aatttgcctc tgccataaaa
    16921 tctccttact tctatccaac caatgcctga attccatact ggattccaac atcaagaact
    16981 gcaacaatga ctccgataat aatagcacag atcactacaa ctgctgtttc ttttgcaatt
    17041 ctttctttcg ttggccagat aattctgtta aattcagctt ttaactcttt gaaccaactt
    17101 gttttctttg ctttgttagt tttctccatg ttttcacttc cttttctctc aaagggaatt
    17161 accggaaatt attttgtttc tttgtgtaat gtgtgagttt tacagaattt gcagtatttc
    17221 tttgtttcca tgcgttcagg atgtgttttc ttatcctttg tcatgttgta attacgctgt
    17281 ttacattctg tacattctaa tgttactctt gtgcgcacaa cttccacctc cattttgttt
    17341 gttgtttaat taaaataagg cataaaaaaa agaccttctt ccaacgctaa agtctagcgt
    17401 gtttatcata tcatccatat gttggtccgt caatagattt ttcgtttttt ttacaataca
    17461 atcttgtttc tttacatata aaacgaaata ataccgcatc aacatctcgg ctgatacggt
    17521 acttattttc aaaaagagag gattaaaaat atatatgaaa aactatgtct ttattatatc
    17581 aggatcctct cttcattcaa tattctgttt ctaaacattt ctaaagaaag tataaacaaa
    17641 tatgaaggaa ttattattta gtgtatttta cctgatatcc acttgtacct ttggatcctt
    17701 ttttaatctt aatatagtaa gttccttttt ttgcctttcc atagatacgg aaaccttttg
    17761 atattgtttt attttttaat cttgctgctc ctgtgactgt tacagaaatc ttgcttttat
    17821 ttccaagtcc tgtgatatcg atatatgtca ttccagtctt cttaactttg atcttatacc
    17881 agtgtgtttt acttgtactt tcattagaca agaatacctg cttcttggat ttcttacgct
    17941 tgatctcttt tgcttttttc ttagatgttc cataactgtc agatgctttt tgatttgcat
    18001 tgatgaaata aaggatctct ccagatgccg catttgcaac gacacgataa gtccctgcag
    18061 caagtcctgt tgttactaca tagttatttt ttgctgcacc cgataatccg gttgctacag
    18121 ttttccatgt ctttccactt aatttctgaa cagaatatgt aacactagaa acatttcccg
    18181 ctgaattttc tttataaact gcgatcgctg caactcctct tttggatact ttaaactgct
    18241 gatatgtagc tgctccagta cttgcactag cgattacatt atttccgcca aaagttctgt
    18301 tattctggct tgatatcact gctgctccaa atgctgcaac cactggtgta tttgcattct
    18361 cacatgctgt ttcaaggtat aatgcatatg ttcctgcttt gactgcacca gcccagatca
    18421 tttttccatt attcgtctgt gaaagtgtca tagccggatt cccattataa cacagtttta
    18481 aacgtccacc atctactcct gcgacctttg ttgcatatgg gataacaacc gttccatcaa
    18541 ttcctacacg aattgttcca actttcacat attcatttgc atcactatat ccttgataca
    18601 acatcatttc ctgatttgcc tgccatttat ttgacagcgt atcaaaatca gacagcgtgt
    18661 aaacattcgt tgcatttaac tgcactgccg ctgcagatgc ttttgcctgt tttgctctta
    18721 ctctttgtga tgctgtgatc gcaggaagtt tgctgtgaat attttctgtt tttgtttttg
    18781 ctcctaccat tacagtccct gtcatcattg caaatgacaa cactatacat aacgttctct
    18841 ttaaaaagtt tttaaccata ttacatctcc ttatttgtat acatatgcac ttgctacagt
    18901 tgatacgtca ctggaatatg ttttatttcc aaatttaact tttggagcca cctgaacata
    18961 atatgttcct ttttttaatt ttttctttcc gttttttgtg atctggaaag agtagctctt
    19021 ctttttagaa actttgactg tttttacttt tttgaatcca gattttgact tctttgatat
    19081 ggaaactgta tactgcttaa tccctcttcc ctttttcaga actactttga tgcctttctt
    19141 cttgtttgtt gtctttcctg aaatagatgg cattgcgaag tagcgatagt tgctccatct
    19201 tccatagtat gtctgattat tacttctgtt tgtatagtag tatcttgcac gatatttata
    19261 cgccatatct tttgacacat tcatagactc ataacccata gatgttgatg tcttagatag
    19321 tactctttta ttctttgcat tatatacttc cagctgtact cctgttgcca tctcaggttt
    19381 attaacctta aatgctacct tcttaatatt agcgtatgct gctgtgatcc caaaattatt
    19441 cttcgctaca tcagcaggtg ttgtgttaac ataaacactg tcaactgacc agttatattt
    19501 ttttcctgtg gaaagatgtg ta
//
LOCUS       DAOMUE010000010        17651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_611_length_17651_cov_2.495226, whole genome shotgun sequence.
ACCESSION   DAOMUE010000010 DAOMUE010000000
VERSION     DAOMUE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 17651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17651
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_611_length_17651_cov_2.495226"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PLC71_01200"
     CDS             complement(<1..841)
                     /locus_tag="PLC71_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HRM01292.1"
                     /translation="MNTPISWIKAYVPDLDCTVQEYVDKMTLSGSHVENAVYLDKNLE
                     KIVVGRIEKIEKHPDADKLVICQVNVGDEEVQIVTGASNVFKGAMVPVVLDGGRVAGG
                     HDGSPNPENGIKIKKGKLRGVPSYGMMCSIEELGSTRDMYPEAPEDGIYIFDESKDVK
                     PGDDAVAALGLRDAVVEFEITSNRVDCFSMIGMAREAAATFEKPFYAPEVKEVGNNEK
                     AEDYISVEVEATDLCPRYTARIVKNIKLAPSPEWIQRRLAAMGIRPINNIVDITNYVM
                     EEYG"
     gene            complement(1020..2039)
                     /gene="pheS"
                     /locus_tag="PLC71_01205"
     CDS             complement(1020..2039)
                     /gene="pheS"
                     /locus_tag="PLC71_01205"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRM01293.1"
                     /translation="MKERLEAIKKAALEKIEQADAMDSLNDIRVSVLGKKGELTQVLK
                     GMKDVAKEERPIVGQMVNDTRAVIEEALEKEMTLLKKKVREEKMKREVIDVTLPGKTH
                     EKGHRHPNQIALEDLERVFIGMGYEVVEGPEVEYDKYNFEMLNIPANHPAKDEQDTFY
                     INKDIVLRTQTSPVQARIMETGQMPIRMIAPGRVFRSDEVDATHSPSFHQVEGLVVDK
                     GITFADLKGTLEQFAKEFFGPDTKVKFRPHHFPFTEPSAEVDVTCFKCGGKGCRMCKG
                     SGWIEILGCGMVHPKVLSDCGIDPEVYSGFAFGIGLERVTLLKYEIDDMRLLYENDAR
                     FLKQF"
     misc_binding    complement(2131..2361)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2399..3280)
                     /locus_tag="PLC71_01210"
     CDS             complement(2399..3280)
                     /locus_tag="PLC71_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM01294.1"
                     /translation="MKKEQIKGSLSLFLAAIIWGAAFVAQSVGMDYVGPFTFNCVRTL
                     IGGIVLIPCIAFLNRGKVRKKTNFTEKKRLLLGGICCGVALATGSTLQQFGIMYTTVG
                     KAGFITAFYIIIVPILGLFLGKKCGLSVWISVVIALAGLYFLCITDGFSIGKGDIYVF
                     LGAIAFSIHILVIDYFTQFNDGVKMSCIQFFVCGILCFVPMMLFEHPEISMILLAWKP
                     ILYAGVMSCGVAYTLQIVGQKNMNPTVASLILSLESVTSVIAGFLVLHQNLSHRELIG
                     CGLMFIAIVLAQLPQKI"
     gene            complement(3447..5537)
                     /locus_tag="PLC71_01215"
     CDS             complement(3447..5537)
                     /locus_tag="PLC71_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HRM01295.1"
                     /translation="MKETGKVFGVLQRVGRAFMLPIAVLPVAGLLLGIGRSLTNETML
                     RAYHLMKIMGPGTILYGVIKVGSDCGSIIFGNLPLIFAMGVSIGMAKAEKAVAALSGA
                     LSFFVMHEAISSMLSLYGDTYTMHAGATDQILGITSLQLGVFGGILVGLGVGYLHNRF
                     YKIKLPEVLSFFGGTRAVPVICTIVYVGVGILMFFIWPPIQNLMYSIGGIVRASGYLG
                     TFIYGVMERALIPFGLHHVFYLPFWQTSLGGTMEVAGKMIDGAQNIFFAQLADPATTH
                     FNVEATRFMAGKFPLMIFGLPGAAFAIYRCARPEQRKVVGGLMISAALTSMLTGITEP
                     LEFTFLFIAPGLYGIHCVFAGLAYMMMHVLKITVGLTFSGGLIDLFFFGIMQGQEKTN
                     WMMVIPLGIVYFIVYYSLFHFLILKFDIKTPGREEEPAQEYEEEPEYEYREDFSEELL
                     AKIAAGLGGKKNIGDIDCCVTRLRCGLKDISLVDDAMIKETGSRGILKRGNSIQIIYG
                     PYVTVLKSELEDYLRTRRAKKEIKQLLKENTHSKDKMQQESIACPIKGCVIELEEVGD
                     EAFSGGMLGEGFAVKPEEGKLYAPVDGIIAAVFRTKHALAMESKDGAEVLIHLGLDTV
                     KRGGKGFYMKVEAGQKVKKGELICEFDLEDMRVDGYVMTTPVVISNNAEYASISLKHI
                     GECNVGEEILTLYK"
     gene            complement(5596..6531)
                     /gene="manA"
                     /locus_tag="PLC71_01220"
     CDS             complement(5596..6531)
                     /gene="manA"
                     /locus_tag="PLC71_01220"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HRM01296.1"
                     /translation="MGHILRMNPVFKEMIWGGTKLKDIYGYEIPSDHTGECWAISAHK
                     NGDCTIADGEYKGKTLSWLFENHRELFGNIEGDQFPLLVKIIDAKNDLSVQVHPDDAY
                     AKEHENSLGKTECWFVLQADEGTKMVMGHHAKTKDEFVKAIENDDYDNLLNSFKIKAG
                     DFFYIPSGTLHAICSGSLIYEAQQSSDITYRVYDYHRKDADGNERQLHVQQSIDVTNV
                     PADIDQNKEFPQTILENGTKTRYVDSEFFKVDRYQLNGKNVIKNDEPFMMVSIIEGTG
                     IFEGNEVKKGDHFVICSDQKEVTFDGDMEVMISTL"
     gene            complement(6575..6961)
                     /locus_tag="PLC71_01225"
     CDS             complement(6575..6961)
                     /locus_tag="PLC71_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HRM01297.1"
                     /translation="MAQSINTRVDVVVEATSYQGLTNYGKIMVGDKGFEFFNTRNVND
                     YIQIPWEEVDYVIASVMFKGKKIPRYAIQTKKNGTFSFSSKEPKKVLRAINQYIPSER
                     LVRSLSFFDVLKRNFMPSKKNAKIKK"
     gene            complement(7129..8043)
                     /locus_tag="PLC71_01230"
     CDS             complement(7129..8043)
                     /locus_tag="PLC71_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006438934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HRM01298.1"
                     /translation="MAEKKVQLSKKDRLSVALRSTFLQGSWNYERMQNGGWCFAMIPA
                     IKKLYTTKEDQIAALKRHLEFFNTHPYVASPVIGVTLALEEDKANGAPVEDSAIQGVK
                     VGMMGPLAGVGDPVFWFTARPILGALGASLAMGGSILGPILFFVLWNVIRWAFMWYTQ
                     EFGYNVGTKITEDLSGGLLQKVTKGASILGMFVLGALIERWVSINFTPVVSKVTLSKG
                     AYIDWAKLPAGAEGIKTALTQQASGMALDPTKVTTLQDNLNSLIPGFVPLLLTLLCMW
                     LLKKNVSPIIIIIALFIIGIGGHVIGLL"
     gene            complement(8064..8867)
                     /locus_tag="PLC71_01235"
     CDS             complement(8064..8867)
                     /locus_tag="PLC71_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004442480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HRM01299.1"
                     /translation="MIMSVISMVLVVIVAFLAGMEGVLDEFQFHQPLVACTLIGLVTG
                     QLVPCIILGGSLQMIALGWANIGAAVAPDAALAAVASAIILVQGGQGRAGVDTAIAVA
                     IPLAVAGLFLTMIVRTLSVICVHQMDAAAAKGSFKGVEAWHIIAVCLQGIRIAIPAAA
                     LLAIPSSAVAAALNSMPAWLTDGMSIGGGMVVAVGYAMVINMMATKEVWPFFAIGFCL
                     AAVSDLTLIALGVIAVSMALIYLKLSENAGSGNGGSNTGDPLGDILNDY"
     gene            complement(8894..>9759)
                     /locus_tag="PLC71_01240"
     CDS             complement(8894..>9759)
                     /locus_tag="PLC71_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM01300.1"
                     /translation="PEDIKKQMEDAIAGFENQDEVLIMVDLWGGTPFNQANGLIAGHE
                     DKWAIVAGLNLPMLIDAYASRMMMDTAHEVAAQISGSGKEGVRIYPESLEPKKEEAAP
                     AAVAAPQGAIPEGTVLGDGHIKIGLTRIDTRLLHGQVATTWTKMVNPDRIIVVSDAVS
                     KDDLRKRMIIEAAPPGVKAHVIPIWKMIEVSKDPRFGETKAMLLFETPQDVLKAIEGG
                     VDIKEVNLGSLAHSTGKVVVTKAVAMGKEDVETFEKLIDKGVTFNVRKVPSDSPENMG
                     EMLKKAKAELK"
     assembly_gap    9760..9769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9770..9878)
                     /locus_tag="PLC71_01245"
     CDS             complement(<9770..9878)
                     /locus_tag="PLC71_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455841.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit EIIAB"
                     /protein_id="HRM01301.1"
                     /translation="MVGIILATHGDFAKGILQSGSMIFGDQPNVAAFTLQ"
     gene            complement(10103..10552)
                     /locus_tag="PLC71_01250"
     CDS             complement(10103..10552)
                     /locus_tag="PLC71_01250"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM01302.1"
                     /translation="MGDLIGKVAIGMIEKELKVTLPQDEAAFIALHIVNAELDLDMTE
                     MVSMTKLVNDILKIVDKHFGEQIDKESVFYERFITHLKFFAQRVYIGKEVKSDDTEFQ
                     EIIRNKYHECIECVDEIKDYVKKTCNHDITDEELMYLTVHIKRVTTR"
     gene            complement(10540..10908)
                     /locus_tag="PLC71_01255"
     CDS             complement(10540..10908)
                     /locus_tag="PLC71_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
                     /protein_id="HRM01303.1"
                     /translation="MQITKIVNNNIVISEDKNHKEVVLMGKGLGFQKHKGDEIKLSQI
                     EKTYVMKDHGMTQRFQEMLTDIPIERVKICNKIIQYAKDTLQKNINDNIYVSLTDHIN
                     FAIERVEMGVPFQNPFLWET"
     gene            11325..12227
                     /locus_tag="PLC71_01260"
     CDS             11325..12227
                     /locus_tag="PLC71_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRM01304.1"
                     /translation="MLKLISMIGSVIDKAQKDHVTAFSAQAAFFTILSFFPFMIVLLS
                     LTRYFPITPNDMIELVRTYLPPKYSGMVVPIIRSLNGKLTTTYMSITILTLLWSASKG
                     ILSLMTGLNTIHEIPEKRNYFVLRFISSIYIGLFAIAVLFGLILLLFGNSLLIQLYRF
                     EPVLENKHVFFATIRFFLAFFTFMVVFIIMYRFLPSENFKTKQILPGAFFSSAAWFVL
                     SFFFSMYFDNFSFFSTMYGGLTSILLTLFWLYFCMMMLFIGAEINHYFISQSSKKPVK
                     DLTFHMNLNRKNPAQQDSFLSFIK"
     gene            complement(12217..13437)
                     /locus_tag="PLC71_01265"
     CDS             complement(12217..13437)
                     /locus_tag="PLC71_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRM01305.1"
                     /translation="MKNLLVAQSGGPSAAINATITGVIDAGIESGRVGHVYGALNGIK
                     GVLNENFVNLDEVCDTKEKRDLLAITPAAALGSCRWKLGNPKENAEEFEEIIRILRKN
                     DIGYFIYTGGNDSMDTVYKLSVYCEEHNIDDIKVMGAPKTIDNDLGETDHCPGFGSAA
                     KFIATAFTEIACDCFVYDVPSVTIVEVMGRNAGWLTAASALARIEGRKVPQLIYLCEK
                     AFDEDRFIEDVNEALKKENNIIIAVSEGVKTADGNYVGEETKSGKEDVFGHKYLSGVG
                     KYLEGLVGEKIGCKVRSVELNILQRCAGYMLSETDIMESRNLGAFAAVSAIRGKSGKM
                     SAVRRISDDPYQVEIVLSDLSKIANYEKKVPMEWINEEGNDIKDELLTYLKPLIQGEV
                     QIPYENGIPKRFIL"
     gene            13656..15428
                     /locus_tag="PLC71_01270"
     CDS             13656..15428
                     /locus_tag="PLC71_01270"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HRM01306.1"
                     /translation="MNLNFMKLREQMKNQKVSYYLVPSEDPHQSEYVDDYFKCRQYIS
                     GFTGSAGTFLAGCEEGWLWTDGRYFTQAEGQISSDITLMKQGVSGVPTILEFLKEHLQ
                     EGDTLGVNGFTISASYGKKIAKLVKQHGASFRFDLRFVEELWAKDDRPAITKSTIYRH
                     DIKYSGEHTDSKLARVREKMKELDANAFFLSSLPDIAWLFNLRGDDIACTPLFYSYAW
                     ITIDKCFLFLRKDCISAVAFQRFKEHGISILDYTEVSAFLKDQHETVLLNPDLTNYLH
                     YNLLFKCKIIEDKNPTELMKAIKNDIQIDHLKACHINDGIAMTKFMYWLKKNVGKIPM
                     TERMISDHLEEERKKLPDYMGPSFETICAYKDHAAMMHYQSTEESDVDVKAEGMLLID
                     SGGQYYGGTTDVTRTFILGPISEEERKYFTLVLKSMLTLANAKFLFGCRGSNLDILAR
                     EPLWEDGVDYRCGTGHGVGYFLGVHEGPNAFRWRSNPENLDAVLQPGMVITDEPGVYV
                     PGKYGIRTENMLICKKWQQNEYGAFLHFEPLTLVPIDLDGVDLSLFNEKEKQLLTDYQ
                     QFVYDTLSPHLNEEESAWLHTLTL"
     gene            complement(15509..16339)
                     /locus_tag="PLC71_01275"
     CDS             complement(15509..16339)
                     /locus_tag="PLC71_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM01307.1"
                     /translation="MKKILASLMMVGLIFCLGGCNVPWMKQKTEKTKATKVIQADKVE
                     GTMKGMKLKVGVSNDMAPFSYYDSEQKKITGFDIDLLDKLSEYLGFEYELYPMNMKKL
                     EQKIKNKELDLAIAGISITDERQREFSFTDTYYETYLQIVVRKDSQITDRKEITEKKI
                     GVVEGTSSAQYAEDYLSEDNKITYYKNITKVWNDLEKGTIDATIYDTTGIQNYMQEHA
                     DNTNLSVLNEQLNSEESNYGIMFVKGYKYLDQFNVALQVLNNDGTYQKLKEQWIKTKE
                     "
     gene            16485..17261
                     /gene="sigG"
                     /locus_tag="PLC71_01280"
     CDS             16485..17261
                     /gene="sigG"
                     /locus_tag="PLC71_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HRM01308.1"
                     /translation="MARKKVEICGVNTSSLPLLSEEEKEDLFERIEQGDLLAREHYIK
                     GNLRLVLSIIQRFSGSSENADDLFQVGCIGLMKAIDNFDRNLNVKFSTYAVPMIIGEV
                     KRYLRDNHSMRVSRSLRDTAYKAINAREVLTKKLNHEPTIDVIAKEIDMKKEDVLFAL
                     DAIATPLSLYEPVYQDGGDTLYLMDQVKDKKNKEDSWVESIALKEAMEHLPDREYDII
                     KMRFFEGKTQTEVADEIAISQAQVSRLEKNALKHMKNHLV"
     gene            complement(17274..>17651)
                     /locus_tag="PLC71_01285"
     CDS             complement(17274..>17651)
                     /locus_tag="PLC71_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HRM01309.1"
                     /translation="EVVLEKLRKHIGLRHYEEDEELLEDVIIENKQAMEMTDIYHNIL
                     RSTMSLFGSIIDNNLNQVMKFLAAITIILELPTMISGLYGMNVNGVGMPFATKAYGFA
                     AISIFSLIVCLIAVYILKKKHIL"
BASE COUNT         5089 a         3898 c         2709 g         5945 t
ORIGIN      
        1 gtccgtactc ttccataacg tagtttgtga tatctacgat attgttgatt ggacggatac
       61 ccatagctgc aagtcttctc tgtatccatt ctggcgatgg agctaacttg atattcttta
      121 cgattcttgc tgtgtatctt ggacataaat cagtagcttc tacttctaca gagatatagt
      181 cttctgcttt ttcgttattt ccaacttctt ttacttctgg agcatagaat ggcttttcaa
      241 atgtagctgc tgcttctctt gccataccaa tcatgctgaa acagtctaca cgattagaag
      301 taatttcaaa ttctacgact gcatcacgaa gtcctaatgc tgctactgca tcgtcacctg
      361 gttttacatc tttgctctca tcaaaaatgt aaataccgtc ttctggtgct tctggataca
      421 tatcgcgagt gcttcctaat tcttcaatag aacacatcat accgtaagaa ggaactccac
      481 gaagtttacc ttttttaatc ttaattccat tttcaggatt tgggcttccg tcatgtccgc
      541 ccgcaacacg tccgccatct aaaacaacag gaaccatagc tcccttaaat acgttagatg
      601 cgcctgttac aatctgtact tcctcgtcac ctacatttac ctgacagata actaatttat
      661 ctgcatctgg atgtttctcg attttttcaa ttcgtccaac aacgattttc tccagattct
      721 tatcaaggta tactgcattc tctacgtggg aaccagacag agtcatcttg tctacatatt
      781 cctgtacagt acaatccaaa tctggaacat atgctttaat ccatgaaatt ggtgtattca
      841 tttttgtctc cttttatatc tataaacttg aatatatttt gaatttatct taccgagata
      901 attctagttt ctcatcatgt taaccttttt acatttcatc gaattaggtt tctcggtctt
      961 actaaaaaat atttgaataa ttctttctgc tgattgatga aataactttt caacacgctc
     1021 tagaactgct taaggaatct ggcatcgttc tcatataaca gacgcatatc atcaatctca
     1081 tattttaaga gtgtaactct ctcaagaccg attccgaatg caaatcctga atatacttct
     1141 gggtcgattc cgcaatcgct taatacctta gggtgtacca ttccacatcc aagaatctcg
     1201 atccatccgc ttcctttaca catacggcat ccttttccac cacatttgaa acatgtcacg
     1261 tctacttccg cacttggctc tgtaaatggg aaatgatgtg gacggaattt tacttttgta
     1321 tctggtccga agaattcttt tgcaaactgt tctaatgttc cttttaagtc tgcaaatgtg
     1381 attcctttat ctacaactaa tccttctacc tgatggaaag atggagaatg tgttgcatct
     1441 acttcgtcag aacggaatac acgtccaggt gcgatcatac ggattggcat ctgtcctgtt
     1501 tccatgatac gagcctgtac aggagatgtc tgtgttctta acacgatatc tttgttgata
     1561 tagaatgtat cctgttcatc ttttgcagga tgatttgcag gaatatttaa catttcaaag
     1621 ttgtatttat cgtattctac ttctggtcct tctacaactt cgtatcccat accgataaat
     1681 actctttcta aatcttccag cgcgatctgg tttggatgac gatgtccttt ttcatgagtt
     1741 tttccaggta atgtaacgtc gatgacttca cgtttcatct tttcttcacg aacttttttc
     1801 tttaataatg tcatctcttt ttctaatgct tcttcgatga ctgctctggt atcattgacc
     1861 atctgtccaa cgatcggacg ttcttctttg gctacgtcct tcattccttt taatacctga
     1921 gttaattcac cttttttacc cagtacagat acacggatat catttaaact atccattgca
     1981 tctgcttgct cgattttctc caaggcagct ttcttgattg cctctaatct ctctttcatc
     2041 tgcttagttc tcctttattg atcttttcta tattctcgga tcaggttact ttttcttctg
     2101 tattcaggca aaagaaaaag aacccgtccg caaagggacg agttcttaat atatactcgc
     2161 ggtaccaccc taattcctat ggtatcgtta aacttcttct taattaccat aagcacttaa
     2221 gtctctgcgt aacgggcaga ctccgttatc tcctactgat acatgatatg aaaatgattg
     2281 ttgcatcaca ttcaaagata cagctacggt gtgaaattcg gaaacttttg gtttcttact
     2341 gtgcaccatc aatgccttta acgttattat tctataacat tcttatggca tttgtcaatt
     2401 aaattttctg tggaagttgt gccaatacga tcgctataaa catcaaccca caccctatca
     2461 actctctatg gctcaaattc tgatgcagaa ccaaaaatcc agcgatcaca gaggtcacag
     2521 attccagact aaggatcaac gatgctaccg taggattcat attcttctgc cccacgatct
     2581 gtaatgtata agcaactcca catgacatca ctcctgcata taagattggt ttccatgcaa
     2641 gtaagatcat gctgatctcc ggatgttcaa aaagcatcat tggaacaaaa caaagaatcc
     2701 cgcagacaaa aaactgaata catgacattt tcactccatc attaaattgt gtaaaataat
     2761 cgatcactaa aatatgaatc gaaaatgcaa ttgcacctaa aaatacatag atatcccctt
     2821 ttccaatcga aaatccgtcc gtaatgcata aaaaataaag tcctgcaagt gcgatcacaa
     2881 cgctgatcca gacggataat ccgcactttt ttcctaaaaa taatcccaaa attggaacaa
     2941 tgataatata aaatgctgta ataaatcctg cttttcctac agttgtatac atgattccaa
     3001 attgctgcaa ggtgcttcct gtagctaatg caactccgca acagattccg cctaatagca
     3061 atctcttttt ctccgtgaag tttgttttct tccttacttt tcctctgttt agaaacgcaa
     3121 tacacggaat caatacaatg cctccgatca atgtacgaac acaattaaat gtaaatggtc
     3181 caacataatc cattccaaca ctttgtgcaa caaatgcagc gccccatatg atcgcagcta
     3241 aaaataatga taatgatcct ttgatctgtt cttttttcat acttttcaaa ccttctcttt
     3301 cgttagcata ttgaatttat tttactacgg tagttttttg tctgcaagta aaaagaaaga
     3361 atgcatctta tgatctgata tcgatcataa atacattctt tctttttaag tttaagaatt
     3421 gtatctatat ttaattttat agttacctat ttataaaggg tcagtatttc ttcccctacg
     3481 ttacactctc caatatgttt caatgaaata cttgcgtatt ccgcattatt actgataact
     3541 accggagtcg tcataacata tccatctact ctcatatctt ctaagtcgaa ctcacagatg
     3601 agctctcctt tttttacttt ctgaccagct tctactttca tatagaagcc ttttccacct
     3661 cgctttactg tatcaagccc aagatgtatc aaaacttctg ctccatcttt ggattccata
     3721 gcaagggcat gttttgtcct aaacactgct gcgatgatcc catctactgg tgcatatagt
     3781 tttccttctt ctggtttaac tgcaaagcct tcccctaaca ttccaccaga aaatgcctca
     3841 tctccaactt cttctagttc gatcacacat cctttgatcg gacaggcgat actttcctgt
     3901 tgcattttat ctttggaatg tgtattttct tttagtaatt gtttgatttc tttctttgct
     3961 cgtcttgtcc ttaaataatc ttccagttct gattttagca ccgttacata tggaccataa
     4021 atgatctgaa tgctgtttcc tcgtttcaaa attccccttg atcctgtttc tttgatcatg
     4081 gcatcatcca ccagacttat atctttcagt ccacatcgaa gtcttgtaac acaacagtca
     4141 atatcaccga tattcttttt gcctccaagt cccgctgcga tctttgctaa taattcttct
     4201 gaaaaatctt ctctgtattc atattctggc tcttcttcat attcttgtgc tggttcttct
     4261 tcccttcctg gtgttttgat atcaaatttc aagatcagaa aatggaataa ggaataatac
     4321 actatgaaat aaacaattcc cagtggaatg accatcatcc agtttgtttt ttcttgtcct
     4381 tgcatgattc caaaaaagaa cagatcgata agtcctccag aaaatgttaa tccaactgta
     4441 atcttcaata catgcatcat catatatgca agtcctgcaa aaacacaatg tattccatat
     4501 aatccaggtg caataaataa aaatgtaaat tccaacggtt ccgtaattcc tgtgagcatt
     4561 gatgttaatg ctgctgaaat cataagtcca ccgacaactt ttctttgttc tggtctggca
     4621 caacggtaaa ttgcaaatgc cgctcctggc aatccaaaga tcatcaacgg aaattttcct
     4681 gccataaatc ttgttgcttc cacattaaaa tgtgttgttg ctggatctgc taattgagca
     4741 aaaaagatat tttgtgcccc atcgatcatt ttcccagcaa cttccatcgt tccacctagc
     4801 gatgtctgcc agaacgggag ataaaataca tgatgaagtc caaatgggat caatgcacgt
     4861 tccatcactc cataaataaa cgtccctaaa tagcctgaag ctctcacaat tcctccaatg
     4921 gaatacatca gattctggat cggaggccag ataaagaaca ttaatattcc aactccaaca
     4981 tatacaatgg tacaaataac cggcactgcc cttgtcccgc caaaaaacga taaaacttct
     5041 ggaagtttta ttttataaaa acggttatga agatacccga caccaagtcc gacaagaatt
     5101 cctccaaata cacctaactg cagtgatgtt attccaagaa tctgatccgt tgctcccgca
     5161 tgcattgtat atgtatcacc atacaatgat aacatcgatg aaatagcttc gtgcataacg
     5221 aaaaaagaca atgcccctga taatgctgct accgctttct ctgcctttgc catcccaatt
     5281 gaaactccca ttgcaaatat caacggaaga ttgccaaata taatacttcc acaatcactt
     5341 ccaactttaa ttactccata caaaatagtc cccggaccca tgatcttcat cagatgatag
     5401 gcccgaagca ttgtttcatt tgtcaaagat cgtccaattc caagcaaaag acctgcaact
     5461 ggcaaaacag cgattggaag cataaatgca cgtccgactc tttgcaagac gccaaatacc
     5521 tttcctgttt ccttcatata aaacttcctt attctatata ttcttataat ccttctttat
     5581 ctattatggt catgatcata atgtggaaat catgacttcc atatctccat caaaagtaac
     5641 ttctttctga tcggaacaga ttacaaaatg atcacctttc tttacttcat ttccttcaaa
     5701 aattcctgtt ccctctatga tgcttaccat cataaatggt tcgtcattct tgatcacatt
     5761 ttttccattc aactgataac gatccacttt aaagaattca ctgtcaacat atcttgtctt
     5821 tgtaccattc tcaagaattg tctgcggaaa ttctttgttc tgatcaatat ctgctggtac
     5881 atttgtcaca tcaatagatt gctgtacatg aagctgcctt tcatttccat ccgcatcttt
     5941 tctatgataa tcataaacac gatatgtaat atcagaagac tgttgtgctt catagatcag
     6001 cgatccgctg cagatcgcat gtaatgttcc agatggaata tagaagaaat ctcctgcttt
     6061 gatcttgaat gaatttaaca gattgtcata atcatcattc tcgatcgctt tgacgaattc
     6121 atcttttgtc tttgcatggt gtcccatgac catctttgtt ccttcatccg cttgaagaac
     6181 aaaccaacat tctgtctttc ctaaagaatt ctcatgttct ttcgcatacg catcgtctgg
     6241 atgcacctgt acagacaggt cattttttgc gtcaatgatc tttacaagta atgggaactg
     6301 gtctccttcg atatttccaa ataattctct gtggttttca aataaccagg ataatgtttt
     6361 tcctttatat tctccatctg cgatcgtaca atctccattc ttatgtgcag aaattgccca
     6421 acactctcct gtgtgatcac tcggaatctc atatccataa atatctttta attttgttcc
     6481 accccagatc atttctttaa aaactggatt cattcttaaa atatgtccca tagccatctt
     6541 ctccttcttt accttgtaac tccaaaaatt acatttattt tttaatcttc gcattcttct
     6601 ttgatggcat aaaattacgt ttcaacacat caaaaaagct taaagaacgc accagacgtt
     6661 cggatggtat atactggttg atcgcacgca acactttctt aggttctttt gatgaaaatg
     6721 aaaatgttcc atttttcttt gtctggatcg cataacgtgg gattttcttt cctttaaaca
     6781 tcacagatgc gatcacataa tcaacttctt cccatggaat ctgaatataa tcattgacat
     6841 ttcttgtatt aaagaattca aatcctttgt ctccaaccat gatctttcca tagtttgtaa
     6901 gtccctgata cgatgttgct tccacaacaa catcaactct tgtattaatt gattgtgcca
     6961 taagctttcc tccgattctg taggttcttc tttatttatt ctatacaatt ttctttgctt
     7021 atacaagatg acttttcgtt cattcttacc gatatgtttt ttatattctt cttatataag
     7081 caaaaagccc ggacttccgg gccttttgta tatgtgctag atcatctctt ataataatcc
     7141 gataacgtgt ccaccaatac caataataaa taatgcgatg atgatgatga ttggagaaac
     7201 gtttttcttt aataaccaca tgcataacag tgttagcagt aatggtacaa atccagggat
     7261 caatgagttt aagttatcct gaagagttgt aacttttgtt ggatcaagtg ccataccgga
     7321 tgcctgctgt gttaatgctg ttttgattcc ttctgctcct gctggaagtt tagcccaatc
     7381 gatgtaagct cctttactta atgtaacctt agatactaca ggtgtaaagt tgatacttac
     7441 ccaacgttcg atcaaggcac caaggacgaa cataccaagg atagatgctc cttttgttac
     7501 tttctgaagt aatccacctg ataagtcttc tgtgatcttt gttcctacat tgtaaccaaa
     7561 ttcctgtgta taccacatga atgcccaacg aattacattc cataatacga agaataagat
     7621 tggtccaagg atacttccac ccattgcaag ggaagctcct aaagctccaa ggataggtct
     7681 tgctgtgaac cagaatactg gatcaccaac accagctaaa ggtcccatca ttccgacttt
     7741 aacaccttgg atcgcactgt cttctacagg tgcaccattt gctttatctt cttccagagc
     7801 cagtgtaaca ccgataactg gagatgcaac atatggatgt gtattgaaga attccagatg
     7861 acgttttaat gctgcgatct gatcttcttt tgttgtatat aatttcttga tagctgggat
     7921 cattgcgaaa caccaaccac cattctgcat acgttcatag ttccaagaac cctgaaggaa
     7981 agtagaacgt aatgctacag acaatctatc ttttttactt aattgaactt ttttctctgc
     8041 catttttctt acctccttct taatcaataa tcatttaaaa tatcgcctaa tgggtcacca
     8101 gtattgcttc caccgttacc agatcctgca ttctcagaca gtttcaggta gatcagtgcc
     8161 atagatacag cgataacacc taatgcgatc agtgttaagt ctgaaacagc agccagacag
     8221 aaaccaattg cgaagaaagg ccatacttct tttgtagcca tcatgttgat aaccattgcg
     8281 taacctacgg ctacgaccat accaccaccg atggacatac catctgttaa ccatgctggc
     8341 atactattta atgctgcagc tacggcagat gatggaattg caagtaatgc tgctgctggg
     8401 atcgcgatac ggataccctg taaacaaact gcaatgatat gccatgcttc tacacctttg
     8461 aagctacctt ttgcagcagc tgcatccatc tgatgaacac agataacgga aagtgtacga
     8521 acgatcattg ttaagaataa tcctgctact gctaatggga ttgctactgc gatggcagta
     8581 tcaacaccag ctcttccctg tccaccctga acaagaatga ttgcagatgc aacggctgct
     8641 aatgcagcat ctggggcaac ggctgcaccg atgttagccc atcctaaagc gatcatctga
     8701 agactacctc caaggatgat gcaaggaaca agctgtccgg ttacaagtcc gattaatgta
     8761 caagctacta atggctgatg gaactggaat tcatccaaaa caccttccat accagccagg
     8821 aatgcaacga taacaactaa taccattgaa ataactgaca tgataattcc tcctatatat
     8881 ttttctaaat tgattattta agttctgctt tcgctttctt aagcatttca cccatgtttt
     8941 ctggagaatc gcttggtact ttacgtacat taaatgtaac acctttgtcg atcagtttct
     9001 cgaaagtttc tacatcttct ttacccatcg caacagcttt tgttactact actttacctg
     9061 ttgaatgtgc aagtgatcct aagttaacct ctttgatgtc aactccacct tcgattgctt
     9121 ttaatacatc ctgaggtgtt tcaaacagaa gcattgcttt tgtctcacca aatcttggat
     9181 cttttgatac ttcgatcatc ttccagattg ggataacgtg tgcttttact cctggaggtg
     9241 ctgcctcaat gatcattctc ttacgaagat catcttttga tacggcatca gatacaacaa
     9301 tgatacggtc tggatttacc atctttgtcc atgttgtagc cacctgtcca tgtaataaac
     9361 gtgtgtcgat acgagtaaga ccgatcttga tgtgaccgtc tccaagtact gttccttctg
     9421 ggatcgctcc ctgaggtgct gccacagcag ctggtgcagc ttcttctttc ttaggttcta
     9481 gagattctgg atagattctc acaccttctt ttccacttcc ggaaatctga gctgcaactt
     9541 catgtgctgt gtccatcatc atacgagatg cgtatgcatc gatcaacatt ggaagattta
     9601 atccagcaac gattgcccat ttatcttcat gtccggcaat taatccgtta gcctgattaa
     9661 atggagttcc accccataaa tcaaccataa tcagcacttc atcctggttt tcgaatccgg
     9721 caatggcatc ttccatctgt tttttgatgt cttctggtcn nnnnnnnnng ctgtaatgta
     9781 aaagccgcaa catttggctg gtcaccaaaa atcatggacc cggactgtaa gatacccttg
     9841 gcgaaatcac catgtgtagc aagaataatt cctaccattt tttattcctc cttttctata
     9901 ttttcataag ctaattaatt atatatatta taagaaaatt cttataaata aaaaaacctg
     9961 attcaaatcc gtaaatctcc accatccaaa tccccctgca aaaatacttt cacaaagaaa
    10021 cctggaccct ctggaaaatc catgattcaa atcaagttta gcctgcatta ccagtaacaa
    10081 tcctgatcaa caatataaac ttttatcttg tcgtaactcg cttgatatgt acggttaaat
    10141 acataagttc ttcatctgta atatcatgat tgcaagtctt cttcacgtag tctttgatct
    10201 catctacgca ttcaatacat tcgtgatact tattccttat gatctcctga aattctgtat
    10261 catcactttt cacttctttg ccaatataaa ctctttgtgc aaagaacttc agatgcgtga
    10321 taaatctctc atagaaaaca gactccttat caatctgctc tccaaaatgt ttatccacga
    10381 tcttcaaaat atcattcaca agcttcgtca tagaaaccat ctctgtcatg tccagatcaa
    10441 gttccgcatt cacaatatgc aatgcaatga acgccgcttc atcctgtgga agcgtaacct
    10501 tcagttcttt ctctatcata ccgatcgcta ctttcccgat caggtctccc ataaaaatgg
    10561 attctggaat ggtactccca tctcaactct ctcaatcgca aaattgatgt ggtctgtaag
    10621 tgaaacatat atattatcat taatattctt ctgtaatgta tccttcgcat actgaatgat
    10681 cttattacaa atcttcactc tctcgatcgg aatatcagtt aacatctcct gaaaacgctg
    10741 tgtcatacca tgatctttca tgacataagt cttctcaatc tgactcaact taatctcatc
    10801 tcccttgtgc ttttgaaagc caaggccctt tcccatcagt acaacttctt tatgattctt
    10861 gtcctctgaa attacaatgt tattatttac aatttttgta atctgcacaa ctttacctca
    10921 tacttctcta acgaaaagac ttcctgttgc gcattcacaa aaaaaataaa cctcacttat
    10981 aaacttccct tctgtgaaat tcaataagtt ggtttagcct gcactaccag taacaatcct
    11041 cctcgtaatc tcctgttaac ttatgtctac attttaacaa ggtatcttca ctttgtcaat
    11101 agaatattta agaattattt tataatttat ttattttttt aaataattat aattcttttt
    11161 tgactccgtt aggaactata tcacaatttt ctttactatg caataggtct ttttgtctgt
    11221 tttagatatt ttttctcttt tggcgcaatt cctttgcttt tagaatgctt gtataatcaa
    11281 tcaaattatg atattatgag gtatcaatta agaggtataa tattatgtta aaattaattt
    11341 ctatgattgg atccgtcatc gataaagctc agaaagacca cgttacagcg ttttctgcgc
    11401 aggctgcatt ctttacgatc ctatcatttt ttccatttat gatcgtattg cttagtctta
    11461 caagatattt tccgatcaca ccgaatgata tgatcgaact ggtacgcacc tatcttccac
    11521 caaagtacag tggtatggta gtcccgatca tccgatcttt aaacggaaaa cttactacta
    11581 cgtatatgtc gatcacaatt cttacattat tgtggtctgc atccaaaggg atcttatcct
    11641 tgatgactgg acttaatacg attcatgaaa ttcctgaaaa gagaaactat tttgttttgc
    11701 gttttatctc aagtatttat attggacttt tcgcgatcgc agtattattc ggtctgattc
    11761 ttttattatt tggtaattcc ctgctcattc agttatatcg ctttgaacca gtcctcgaga
    11821 ataaacatgt attttttgca acgatccgtt ttttccttgc attttttaca tttatggtcg
    11881 tttttatcat tatgtatcgt tttttaccaa gtgaaaattt caaaacaaag cagatccttc
    11941 ctggagcatt ttttagttct gctgcatggt ttgtattatc atttttcttt tcaatgtatt
    12001 ttgataattt ttctttcttt agtactatgt acggaggatt gaccagtatc ttgttaactt
    12061 tgttctggtt gtatttttgt atgatgatgc tttttatcgg tgcagagatc aatcattatt
    12121 tcatctcaca atcgtcaaaa aagcctgtaa aagacctgac attccatatg aacttaaata
    12181 gaaagaatcc tgcacagcag gattctttct tgtcttttat aaaataaatc gttttggaat
    12241 tccattttca tatggaatct gtacttcacc ttggatcaac ggttttaaat atgtcagtaa
    12301 ttcgtcttta atatcattcc cttcttcatt gatccattcc atcggaactt tcttttcata
    12361 attcgcaatc tttgacagat cagaaagtac gatttctacc tgatatggat catctgaaat
    12421 tcttcgcact gcagacattt ttccactctt tccgcgaatc gcactgacag ctgcaaatgc
    12481 tccaagattt cttgattcca tgatatcagt ctcacttaac atatatccag cacatctctg
    12541 aaggatgttt aattctacag atctgacttt acatccgatc ttctctccta ccagtccttc
    12601 cagatattta ccgacaccag aaagatattt atgtccaaat acatcttctt ttcctgattt
    12661 tgtttcttct ccaacgtaat ttccgtcagc agtttttaca ccttcactta ccgcaataat
    12721 gatgttattt tcttttttta atgcttcatt tacatcttca ataaatcgat cttcatcgaa
    12781 tgctttctca caaagataga tcaactgagg aacttttcgc ccttcgatcc ttgcaagtgc
    12841 agatgctgcc gtcaaccatc ctgcatttct tcccataact tctacgatcg ttacggatgg
    12901 gacatcataa acaaagcaat cacacgcaat ctcggtaaac gcagtcgcga taaatttggc
    12961 tgcagaacca aatccagggc aatggtcagt ctctccaaga tcattatcga tcgtctttgg
    13021 agctcccatt accttaatgt catcaatatt atgttcttca cagtaaacag acagtttata
    13081 tacggtgtcc atagaatcat ttccaccagt atatataaaa tatcctatgt cattctttct
    13141 taaaatacgg atgatctctt caaattcttc tgcattttct ttgggattcc ccagtttcca
    13201 tcgacaggaa ccaagtgccg ctgccggtgt gatcgctaat agatctcttt tttcttttgt
    13261 gtcacatact tcatcgagat ttacaaaatt ctcgttcagc acacctttaa ttccattcag
    13321 agcaccataa acatgaccca cacgtccact ctcaattcca gcatcgatca ctcctgtgat
    13381 cgtcgcattg attgctgcag atggtcctcc tgactgagca actagtaaat tcttcataag
    13441 tttctccttt atacttttat atttgttcaa tcccgattat accgtatttc ttttattttt
    13501 ttcaactttt ttgacaaaat accatatatt tttacatttg ctttttcttt acgaatccct
    13561 attcatctac tataattaac ataaaccgat cctgaaatct catataaaac atatattttc
    13621 aggattttaa tacgacaaaa tggaggtttt tggacatgaa tctaaatttt atgaaacttc
    13681 gcgaacaaat gaaaaaccaa aaggtttctt attatcttgt tccaagtgaa gatccacatc
    13741 aatccgaata cgtagatgat tactttaaat gcagacaata catctctggt tttactggtt
    13801 ctgccggtac ctttcttgct ggatgcgaag aaggatggct ttggactgat ggacgttatt
    13861 ttacgcaggc cgaaggacag atctcttctg atatcacatt gatgaaacaa ggggtttccg
    13921 gtgttcctac gattcttgaa tttttgaaag aacacctgca ggaaggtgat acacttgggg
    13981 taaacggctt tacgatctca gcttcttacg gaaaaaaaat cgcaaaacta gtgaagcagc
    14041 atggtgcatc ttttcgtttt gatcttcgtt ttgtagaaga actctgggcc aaagatgatc
    14101 gccccgcgat cacaaaatcc acaatttacc gtcatgatat caaatacagt ggagaacata
    14161 ctgattcaaa actggcacgt gtcagagaaa aaatgaaaga acttgatgcc aatgcatttt
    14221 tcttatcctc tcttcctgat attgcatggc tttttaactt gcgtggagat gacattgcat
    14281 gcactccatt attttatagt tatgcatgga tcacgataga caaatgtttt ctttttcttc
    14341 gaaaagattg tatttcagca gttgcttttc aacggtttaa ggaacacggc atttccatcc
    14401 tcgattacac agaagttagt gctttcttaa aagaccaaca tgaaactgtt ttgttaaatc
    14461 cagatcttac aaactatctg cattataatc tcttattcaa atgcaagatc attgaagata
    14521 agaatccaac agaactaatg aaagctataa aaaatgacat tcagatcgat catttgaaag
    14581 catgccatat caatgatggg atcgctatga caaaatttat gtactggctt aagaaaaacg
    14641 tcggaaaaat ccctatgaca gaacgtatga tctctgacca tctcgaggaa gaaagaaaaa
    14701 aactacccga ttacatgggc cctagtttcg agacaatctg tgcttataaa gatcatgctg
    14761 caatgatgca ttatcaatcc actgaagaat ccgatgtcga tgtcaaagct gaaggtatgc
    14821 ttctcattga ttctggtgga caatattatg gtggtacaac cgatgtcaca agaactttta
    14881 ttcttggacc aatttcagag gaagaaagga aatactttac actggtatta aaatctatgc
    14941 tgactcttgc caatgcaaaa ttcctgtttg gctgcagagg aagtaatctt gatattcttg
    15001 caagagaacc tctctgggaa gatggcgttg attatcgttg tggaaccgga catggtgttg
    15061 gatattttct tggtgttcat gaaggtccaa acgcttttcg ttggagatcg aatcccgaaa
    15121 atcttgacgc agtactacag ccaggtatgg tcatcacaga tgaaccaggt gtttatgttc
    15181 ctggaaaata cggaatccgt acagaaaata tgctgatctg taaaaaatgg cagcaaaatg
    15241 aatacggtgc tttccttcat ttcgaacccc tgacacttgt accgatcgat cttgacggtg
    15301 ttgacctttc tttatttaac gaaaaagaaa aacaattgtt aactgattac cagcagttcg
    15361 tatatgatac actttctcca caccttaacg aggaagaatc tgcatggctt catacactca
    15421 cactttgaat cttacaagag cctttcttaa caaacaaaat ggcaggaact tttcatctta
    15481 catatagttc ctgccatttt cttatctttt attcttttgt cttgatccac tgctccttaa
    15541 gcttctgata cgtcccatca ttattgagaa cctgaagtgc tacattaaac tgatccagat
    15601 atttgtatcc cttcacaaac ataatcccat aattactttc ctcagaatta agttgttcat
    15661 tcaacacact aagattcgta ttatctgcgt gttcttgcat ataattctgg attcctgttg
    15721 tatcataaat cgttgcatcg atcgttcctt tttcaagatc gttccatact tttgtaatgt
    15781 ttttataata tgtgatcttg ttatcctctg acaaataatc ctccgcatat tgtgcactgg
    15841 atgttccttc tacaacaccg atcttcttct ctgtaatctc cttacgatct gtgatctggc
    15901 tgtcttttct gaccacgatc tgaagatatg tttcataata agtgtctgta aatgaaaact
    15961 ctctctgtct ttcatctgtg attgaaattc ctgcgattgc aagatctaat tctttgttct
    16021 tgatcttttg ctctaatttc ttcatattca ttggatataa ttcatattca aagcccagat
    16081 attcactgag tttatcaaga agatcaatat caaaccctgt gatcttcttc tgctcactgt
    16141 cataatatga aaatggtgcc atatcattcg atacaccaac ctttaatttc attcctttca
    16201 ttgtcccttc aactttatct gcctgaataa ctttcgtagc cttcgttttc tctgtctttt
    16261 gtttcatcca tggaacgttg catccaccaa gacaaaaaat aagtccaacc atcataagcg
    16321 atgctaatat cttcttcatc tttactctcc tttgatcgta tttatcctca tcatagcacc
    16381 tgaaaaaata taagtcaatt tcctcttcca tttttttcaa cgtataaatt ccaccagatc
    16441 tgtacatgat tttattatac gaatcacaaa tggaggaact tattatggca cgaaaaaaag
    16501 tagaaatctg tggcgttaac acttcttctc tccctttact ttctgaagaa gaaaaagaag
    16561 atttatttga gcggatcgag caaggtgatc ttcttgccag agaacattat atcaaaggaa
    16621 atctacgact tgttcttagt atcatccaac gattttcagg aagtagtgaa aatgcagatg
    16681 atctttttca agttggctgc atcggtctta tgaaagccat tgataatttt gaccggaatc
    16741 taaatgtgaa attctctact tatgctgttc ccatgatcat tggtgaagtc aaacgctatc
    16801 ttcgtgataa tcatagtatg cgtgtaagtc gttctcttcg tgataccgca tataaagcga
    16861 tcaatgcaag agaagttctt accaaaaaat taaatcatga gcctacgatc gacgtcattg
    16921 caaaagaaat cgatatgaaa aaagaagatg tcttatttgc acttgatgcg atcgcaaccc
    16981 ctctttctct ttatgaacct gtttatcagg atggaggtga tactctttac cttatggatc
    17041 aggtaaaaga taaaaaaaac aaagaagata gctgggtgga atccattgcc ttaaaagaag
    17101 caatggaaca tcttccagat cgagaatatg atattatcaa aatgcgattc tttgaaggaa
    17161 aaacacaaac agaagttgca gacgaaatcg ccatttccca ggcacaagtc agccgtctgg
    17221 aaaaaaacgc cctaaaacac atgaagaacc acttagtgtg attcttcaat atcttatagt
    17281 atatgtttct tcttcaaaat atacaccgcg atcaaacaga cgatcaaaga aaaaatcgag
    17341 attgctgcaa acccatatgc tttcgtcgca aacggcattc caacaccatt gacgttcata
    17401 ccatacaatc ccgagatcat cgttggtaat tccaaaataa tcgtgatcgc tgccaagaat
    17461 ttcataacct gattcaggtt attatcaatg atcgatccaa acaaagacat tgtacttcgc
    17521 aggatattat gataaatatc cgtcatctcc atcgcctgtt tattctcaat gatgacatct
    17581 tccaacagtt cttcatcttc ttcataatgt ctaaggccaa tatgttttct taatttctcc
    17641 aacactactt c
//
LOCUS       DAOMUE010000011        17456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_624_length_17456_cov_3.041262, whole genome shotgun sequence.
ACCESSION   DAOMUE010000011 DAOMUE010000000
VERSION     DAOMUE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 17456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17456
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_624_length_17456_cov_3.041262"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..556)
                     /locus_tag="PLC71_01290"
     CDS             complement(149..556)
                     /locus_tag="PLC71_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM01310.1"
                     /translation="MFNENLKIIRKAKGYTQEELAIKIHVVRQTISKWEKGLSVPDAD
                     TLSKLADVLEINVSELLGSEIKEEANKNEVAEQLAKISEQLALKNRRHKRIWKVIGVI
                     LLAVIVINMLLVVLGTVTYNEKNADITTIEEKF"
     gene            complement(636..965)
                     /locus_tag="PLC71_01295"
     CDS             complement(636..965)
                     /locus_tag="PLC71_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HRM01311.1"
                     /translation="MDNVKNDRYYIQKIRNDLQFIVQHMKDVDIEELNANEILLDSML
                     FRMIQLSENAKKLSDEYKEKRGYIPWNAMYGLRNRIVHDYGNVDLNIVFETLKNDIPE
                     LLEMIKG"
     gene            complement(958..1434)
                     /locus_tag="PLC71_01300"
     CDS             complement(958..1434)
                     /locus_tag="PLC71_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HRM01312.1"
                     /translation="MMGELKKMRTEKKMTQQQVADLVGISLRSYKSYENDEKKQGSLK
                     YKYILEKLSEINPIDEEHGIIDIEYITEKCGNVFQKYDVNFCYLFGSYAKSKAKPTSD
                     VDLLISANVKGLKFYGLVEEIREALHKKVDVLEINQLKDNLELTQEILKDGIKIYG"
     gene            complement(1743..2078)
                     /locus_tag="PLC71_01305"
     CDS             complement(1743..2078)
                     /locus_tag="PLC71_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis protein"
                     /protein_id="HRM01313.1"
                     /translation="MKIEYNEKEIAAMAEFHKGNRAEGLRLQEEFASEFRKEYADKDH
                     CPCQKACRYHGNCKECVAIHRAHQEHVPNCMRPMINKKLKILSKLTEHTLANEIEAPK
                     EVLRKEFQK"
     gene            2199..3566
                     /locus_tag="PLC71_01310"
     CDS             2199..3566
                     /locus_tag="PLC71_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM01314.1"
                     /translation="MKRIDFENGSVTQNIFAAALPMLVAQILNLLYNIVDRIYIARIP
                     HIGTAALGAVGLCFPLVVIITAFANLFGSGGAPLFSISRGQKKNKQAVHIMNTSFTMV
                     CFSAVVLMLIGLLFARPLLVLFGASKDALVYAFPYMMIYLIGTLPSMIAIGMNPFINA
                     QGYSTIGMFSVAIGAVANLLLDPFFIFVLGFGVRGAAIATVISQCLSASFVLFFLTKK
                     AELKVRFLHKNELSESLGYAKNIISLGTAGFIMQLTNGLVSIVCNNVLSVTGGDIYIS
                     VMTIVSSVRQLVETPIYAMNEGGSPILSYNYGARRPARVRKAMIVMSAMILCYTAVMW
                     SVIIFAPGALIRVFSSDPSLVKDSVPALNQYFAAFIFMDLQYIGQTVFKSLNKKKHAI
                     FFSLLRKVFIVIPLTYFMPYVLHMGTSGVFLAEPVSNVIGGGFCFITMLCTVLPELRR
                     MDNCD"
     gene            complement(3577..5064)
                     /locus_tag="PLC71_01315"
     CDS             complement(3577..5064)
                     /locus_tag="PLC71_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM01315.1"
                     /translation="MNIKRAKQEIKDSIEAYLTKDNYGEYKIPSIRQRPILLMGPPGI
                     GKTQIMEQIARECEIGLVAYTITHHTRQSAIGLPFIREKEYSGQTYSVTEYTMSEIIA
                     SVYEKIEQTGNKEGILFIDEINCVSETLAPTMLQFLQCKMFGNQKVPDGWIIVAAGNP
                     PEYNKSVRDFDVVTLDRMKKIDVEADYEVWKEYAYQAGIHPAILAYLEIRKEYFYRME
                     TTVDGKAFVTARGWEDLSQLLKVYEELGKDIDREVVFQYLQHLKIAKDFANYYELYKK
                     YKEDYGIERILKGQWNQETIGRLKFAAFDEKLSVVGLLNGKLSEMFAESYRQDAYVTE
                     LFGWLKQLKTGKTLSQVVKEIHEDFNEKKKTEKLGKAQEHMLLDVIKTLESYEQISKA
                     EHLEAEAEFERVREEFKKEPAKLEQQIENTTKALDNAFDFMEEAFGQSQEMVVFVTEL
                     NVNYYSVWFIKENGCDKYYQYNKGLLFDEQQAEIMAEMDELERKF"
     gene            complement(5054..6364)
                     /locus_tag="PLC71_01320"
     CDS             complement(5054..6364)
                     /locus_tag="PLC71_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HRM01316.1"
                     /translation="MEENINERLSELGTKILATTRNELYIHMRFLDVALSSFAYVIDP
                     SVNGVGTDGMCMFYHPGAMYRQNRVRMNRAYLHMVLHCILHHVTRRKGRDKTLWNISC
                     DIAVESIIDHWHLKCIHMVQTRLRKDIYGQLERNLKVLTAEGIYRQLVSFGLEEKKIK
                     ELQMEFRVDDHQYWPEDPKQDLHQEVENKWKNISEKTETDMETFSSEESQQAGDLIGQ
                     VQVENRDRYDYREFLRKFSVLREEVSVDPDSFDYIFYSYGLSMYGNMPLIEPQEWKEV
                     KKVEDFAIVIDTSMSCSGDLVKKFLEETYGVLTEAESFFHKVNIHIIQCDEMVQNDQK
                     ITDEKELKEYMEHLELIGEGGTDFRPAFEYVNHLIEQGEFYHLKGLIYFTDGKGTYPK
                     KKPPYQTAFIFMQEEYEDVDVPPWAVKLIVDAEDIDEGEEEHEY"
     gene            complement(6387..7346)
                     /locus_tag="PLC71_01325"
     CDS             complement(6387..7346)
                     /locus_tag="PLC71_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HRM01317.1"
                     /translation="MLKIQYRDAKDGGIEVLRCFSKEDQVEIPSEIEGKRVTKIGDYA
                     FSAHKRKEDEAKEIMIGEDFLGSEDEPMFCGEAVKEVYLPPDTVEIGKYAFYGCVNLT
                     TLGFSDSLLRTGAGIFTGCKLQKIYYDVYEDKKSALRDVVRDTRFPLQVNIRYKEEEK
                     QSRLFYPEYYEEAVENTPARIVETHFHGTGYQYRQSFLRGEIDYRKYDDIFPVAAVQE
                     DLDIVWEILAGRMLMPYGVSDFAWMQYEGYVKQNQCEMMNYLKEEDQMPFINLMAEKN
                     YFSKEGIEAAIDWASRKQKTELLSILMNEQHKRFPKKKKTFEL"
     gene            7515..8318
                     /locus_tag="PLC71_01330"
     CDS             7515..8318
                     /locus_tag="PLC71_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM01318.1"
                     /translation="MERLIVLGTGNAQAIHCYNTCYAMQKGEEYFLVDAGGGNGILSQ
                     LDKAGIPLESIHNIFVTHAHNDHILGMVWMIRMIATSMNKGTYDGTMTIYCHEELVHT
                     IKTLTKLTVGKKFYKHFDERIVFHVIAHGDTIQILGDSFTFFDIGSTKEKQFGFTSVM
                     ENGLRVSFPGDEPYRESLYDFVKDSDWLLHEAFCVYGERDIFKPYEKHHSTVKDACEL
                     AEHLKIPNLVLWHTEDKNMLHRQEKYMKEGKQYYTGTLYIPEDLDVLKL"
     gene            complement(8335..8904)
                     /locus_tag="PLC71_01335"
     CDS             complement(8335..8904)
                     /locus_tag="PLC71_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRM01319.1"
                     /translation="MEEKKRTIVMFGDSLTDYFPMEKLKNIDTQFINSGVAGDTIAEM
                     GARLAYDVFPYKPDIIIMQGGANDFLMSLYPGARVLAERLIRLARRIRQQLPDTKIYI
                     ESMYPAYTKRIGLMPSWAEGKSNKEIQKINTEIQRLCKEDNFEYVDVFDKLIGEDGQL
                     SREYTVDGIHLQENAYDVVAAIIYHLMEG"
     gene            complement(8908..9678)
                     /locus_tag="PLC71_01340"
     CDS             complement(8908..9678)
                     /locus_tag="PLC71_01340"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HRM01320.1"
                     /translation="MGKIYLEGADNTRDLGGLKTTEGAVIKDKQLIRSNRLSRITQKD
                     KILLETEYHLQKILDLRTPMEVEQEPDLEVAGAVYENIPFFMESMVGVSREQETRKQM
                     LHMEEFPEMSDIYTMMIKEEFCRKQISQAVREIMNTKNGAVLWHCTEGKDRCGLLSAT
                     ILFLLDVSEDDVMEDYLKTNKAAITRVEKLKKKLHLAGLHREKIEKIEGYFVAKEEFL
                     NTALKTMKEEYGSINQFMIKGLNISMQQKEDFKQKVLE"
     gene            complement(9703..12345)
                     /locus_tag="PLC71_01345"
     CDS             complement(9703..12345)
                     /locus_tag="PLC71_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HRM01321.1"
                     /translation="MKNKKHIVTGILAHVDAGKTTLSEAILYTAGNLRKIGRVDNGDA
                     FLDTYELERSRGITIFSKQAEVSMNEMDLTLLDTPGHVDFSAEMERTLQVLDYAVLVI
                     SGADGVQGHTLTLWSLLERYKIPTFLFINKMDQDGTQKDQLLKELQERLSSGCIDFGE
                     KGSDEFYEMIAMEDEALLDHYLESGEITESKIREMIAERKIFPCFFGSALKLQGVNEF
                     LSDLAFYTEEREYPEEFGARIFKITRDENGNRLTHLKVTGGNLKVKTVITESDEKINQ
                     IRIYSGEKYETVNELQAGRICAVTGLSDTIPGQCLGAEQGGYEPVLEPVLTYQMILPE
                     ETSASVILPKLRQLEEEEPELHIIWNEELQEIQVQIMGEVQLEILKSLIKERFDVDVE
                     FGQGNIVYKETIADVVEGVGHFEPLRHYAEVHLLMEPLERGSGVVIDTQCSEDVLDKN
                     WQRLIITHLLERDHRGVLTGAPITDIKITLAAGRAHQKHTEGGDFRQATYRAVRQGLR
                     MAQSVLLEPYYKFRLEVPQQMIGRAMTDIEKMQGTFDTPDTAGEMSVLQGIAPVSTMQ
                     GYQKEVLAYSKGMGRLFTTLNGYDICHNEEEIIEASGYDPDRDLENPCGSVFCSHGAG
                     YNVAWNEVPEHMHLESVLAKEIEEEEFDELTQRRAYIEEESIDTEEIDRILEQTFYAN
                     QHNKSKWKKKKTARLVQDYHTSAEKSSVKRDMSKKYLLVDGYNIIYAWDDLKELLDTS
                     VDAARGKLLDEMSNYQGMKGMELIVVFDAYRVKGHETEITDYMNIHVVYTKEAETADQ
                     YIEKFAHTHGRKYDVTVATSDGLEQIIIRGQGCRLLSARELKRDYEEAKAQIRTEYLE
                     NQKNEKTYMMDGISLEKEQPEKEN"
     gene            12565..13095
                     /locus_tag="PLC71_01350"
     CDS             12565..13095
                     /locus_tag="PLC71_01350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM01322.1"
                     /translation="MRDALRDEMIKKICVRDTDNILDVGCGDGTFLHELTRWKDVEGY
                     GIDESEDKILIAKQTWPELHFETGYSDFLSFDDNSFRVITVCDDFHTFKDPQKFVNEA
                     FRVLVPGGRLYIGESALPEALRIVSNIPSYLTSGDDRRHSTYETLEYFKNAGLTKFRV
                     YKKKRLLLISAKKPLE"
     gene            complement(13147..13728)
                     /gene="spoIIR"
                     /locus_tag="PLC71_01355"
     CDS             complement(13147..13728)
                     /gene="spoIIR"
                     /locus_tag="PLC71_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HRM01323.1"
                     /translation="MIKGILFLAIACMIFVAGQRKSNNIIQQNLKSNLIRFHVRANSN
                     SRMDQTYKLKVRDAVIDQISPELSEAKTKEEAFKILKMQKNRIKNTAQEILKKEGVKQ
                     KVNVHFVQEKFPEKNYGQYTFPEGIYDAVRIDLGAAKGHNWWCVMFPDLCVTKDDKVR
                     INNTARKKMEKLLGKKTIRKITKDKYLGWLFKV"
     regulatory      13878..13960
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(14137..15810)
                     /locus_tag="PLC71_01360"
     CDS             complement(14137..15810)
                     /locus_tag="PLC71_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HRM01324.1"
                     /translation="MMNKKWWHNKVAYQIYPKSFKDTNGDGIGDIPGIISKLDYLKDL
                     GVDILWISPMYQSPMVDNGYDISDYYAIDPMFGSMDEMKQLIKEAKKRNMYILMDLVV
                     NHCSSEHEWFQKAMADPEGEYGSYFYIKDGKDGQPPTNWRSYFGGSVWEKIPGYDNKF
                     YLHSFAKEQPDLNWENETVREKIYEMICWWMDQGLSGFRIDAIMNIKKDLTWSDLKPD
                     GPDGLADVYKITGKVEGIGDFLMEMKHRCFEPYDALTVGEAMFVKEDILPQFIGEDGY
                     FSTIFAFEPCHAYRKGKNYMNYDWPQPFDEWREETFHNQEIVAKAGFEANIIENHDQP
                     RGASLFIPEEDYGFYSLSALATIIFCERGLPFLYQGQEIGMSNRQWKYDEFNDLETIN
                     QYQIALKAGMSKEQALEIARHHSRDNARTPMQWSSEENAGFSKGKPWMPVNENYKVVN
                     VAEEEKEYGSILNFYKRLIAFYKSEEYNEILTYGDFRPIYEKEDHIFAYSRSYGCQKL
                     VLICNFSSKEKDIELSEDYENVIFVNYEQTTVIGNTLKMKPYECVIYEM"
     gene            complement(15807..16787)
                     /locus_tag="PLC71_01365"
     CDS             complement(15807..16787)
                     /locus_tag="PLC71_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRM01325.1"
                     /translation="MEYYGTLGPTCCEPTILKKMFDAGMTGIRLNLSHSSLEGSHAWI
                     YHYFEAAKQTNGEKKLMIDLIGPELRIGYLEGDMGLSTGAHVVIGHGGILVPEEIMPH
                     IRRDQEILLDDGKIKLIAERKISPRSWRCRIVCGGVLTARKSIALPGCNINNPTLTEA
                     DLKNLSLAKQYQVTDVMLPFVRNKEDLIILREALKQNNCEDIRIFAKIENMTGVEHLE
                     ELLPYCDYIVIARGDLGNVMPLSELPKVQAYIAKVCKEHNKNFMVVTQMLDSMIRNPY
                     PTRAEVNDIYHAVHQGANALMLTGETAHGRYPVEAMKMLVETANGSLEEL"
     gene            complement(16883..>17456)
                     /locus_tag="PLC71_01370"
     CDS             complement(16883..>17456)
                     /locus_tag="PLC71_01370"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRM01326.1"
                     /translation="TYVESPFKDPNWELVRVKGSKKAFLWVYEKDGYINLNIKVDPEW
                     REFWREAYDAVIPGYHQNKKYWNTIILDGTIPEKDIKKMIKESYDIITDSPTKRIYEA
                     VKQIPKGHVATYGQVAAMAGNPKMSRAVGNALHKNPDPENIPCFRVVNAKGELAGAFA
                     FGGEGKQAKLLEEDGVVVVDGKVDLDVYGM"
BASE COUNT         4621 a         3836 c         2626 g         6373 t
ORIGIN      
        1 acgaaacctt aaattgcgag aattttcagc tatcgattac ctaaatatat aaaccaatac
       61 agtaaaacat acacatcacc ttaaattttc caatcttgtg aattgtccac tctatatact
      121 cgaaatctac agttggccaa ctacaaaatc aaaatttctc ttcaattgta gtaatatccg
      181 catttttttc gttatatgtc accgtgccca aaaccaccag aagcatatta ataacaatca
      241 cagctaacaa tataactcca ataactttcc atatcctctt atgccgtcta ttcttaagcg
      301 ccaattgttc actaatcttc gcaagttgtt ctgctacttc attcttattg gcttcttctt
      361 ttatttcact acccaataat tcactcacat taatctctaa tacatccgct aatttggata
      421 atgtatctgc atctggtacg gatagtcctt tttcccattt gctgatagtt tgtcttacta
      481 catgaatttt gatcgcaagt tcttcttgtg tgtatccttt tgcttttctt attattttca
      541 gattctcatt aaacataatt attgtctctc cttatatttt gatgtattga atttacacca
      601 aaatatgtcg ttgcggcaag caacgtgggt taacattatc cttttatcat ctctaacaat
      661 tctggtatat cattttttaa tgtttcaaag acaatattta aatcaacatt tccataatcg
      721 tgtacaatac gatttctcaa tccatacata gcattccatg gaatatatcc tcttttctct
      781 ttatactcat ccgacaattt ctttgcattt tcagataact gaatcattcg aaacaacatt
      841 gaatccagta atatttcatt tgcattcaat tcttctatat ctacatcttt catatgttgt
      901 actataaatt gcaaatcgtt tcgtattttt tgaatgtaat aacgatcatt ctttacatta
      961 tccatatatt tttatcccat cttttaatat ttcttgtgtt aattctaaat tatcttttaa
     1021 ttgattaatt tctaaaacat ctactttttt gtgtagtgct tctctaattt cttcgactaa
     1081 tccataaaat tttagtcctt ttacatttgc tgaaattaac agatccacat cactggttgg
     1141 ctttgctttc gatttagcgt aagatccaaa aagataacaa aaatttacat catatttttg
     1201 aaatacattt ccgcattttt cagttatata ttctatatca ataattccat gttcttcgtc
     1261 tattggattt atttctgaaa gtttttctaa tatatatttg tatttcaaac ttccttgttt
     1321 tttctcatca ttctcatatg atttataaga acgtaaagat attcctacca aatcagcaac
     1381 ttgttgttgt gtcatctttt tttcagttct catttttttt aactcaccca tcataattgc
     1441 acctcttaat ttataatagt gcaatttttg cacttcgtca attcgaaggt gcaaaaattg
     1501 caccattatt taaattctat aataggtatt tttttcttaa taaatttttt tattctatga
     1561 tcatggctgg cttatctgca tattcatcga gaaaaatcaa tactttcttg ccgtataaaa
     1621 gatttagatc actggataac ttccttgtaa cctttgatat ccctgatctg tccagatgga
     1681 aaggccttta aataaatctc ctcgattctc atattgtctg gaaaatagca ttccttataa
     1741 atctactttt gaaattcctt ccgcaaaacc tctttcggtg cctcaatctc atttgccaat
     1801 gtatgttccg ttaattttga caaaattttt aatttcttat tgatcatcgg gcgcatgcag
     1861 tttggaacgt gttcctgatg ggctctgtga attgccacac attctttgca atttccatga
     1921 tatctacagg ctttttgaca tgggcagtgg tctttgtctg catattcttt tcgaaattct
     1981 gatgcgaatt cttcttgtaa tcttaaacct tctgctctgt ttcctttgtg gaattctgcc
     2041 attgctgcga tttctttttc attatattcg attttcatat actataccta ctcctttatc
     2101 catatagatt ttcttttaat ttgtattata atagaataaa tgaaacgatg ccatgataaa
     2161 taaaaatata atgaaactgg aaagagataa tcttaaaaat gaaacgaatt gattttgaaa
     2221 atggttctgt aacccaaaat atctttgcag ctgcacttcc tatgcttgtt gctcagatct
     2281 taaacttatt atataacatt gttgaccgta tttatatcgc acgaatccca catattggaa
     2341 cagctgcact tggtgctgtt ggactgtgct ttccgctggt tgtgatcatt actgcatttg
     2401 ctaacttatt tggaagtggc ggagctcctt tattttcgat cagccgaggg caaaagaaaa
     2461 ataaacaggc tgtccatatc atgaatacat catttactat ggtttgtttt agtgctgtcg
     2521 tattgatgct cattgggctt ttatttgcac gcccgctttt ggtattattc ggagcttcca
     2581 aggatgctct cgtttatgct tttccatata tgatgatcta tctgatcggt acactgccat
     2641 ctatgatcgc cattggaatg aatccattta ttaatgcaca aggatattca accatcggaa
     2701 tgttttctgt tgcgatcggt gccgttgcca acttactgct tgacccattc tttatttttg
     2761 tattagggtt tggcgttcgg ggtgctgcga tcgcaactgt gatctcacaa tgtctttctg
     2821 cttcttttgt gttatttttc cttacaaaaa aagcagaatt aaaggtacgt tttcttcata
     2881 aaaatgagct gtcagaaagt ttaggatatg caaagaatat catcagcctt ggaactgccg
     2941 gatttatcat gcagttaact aacggtctgg taagtattgt ttgcaacaat gtactttctg
     3001 tgactggcgg agatatttat atctctgtca tgacgatcgt atcaagtgta cgacaacttg
     3061 tggaaacccc gatttatgcc atgaatgaag gcggttctcc gatcttgagt tataactatg
     3121 gtgccagacg tccagctcgt gtaagaaagg ctatgatcgt tatgtctgct atgatcctat
     3181 gctatacagc cgttatgtgg agtgttatca tttttgctcc tggtgcattg atccgtgtat
     3241 tcagctctga tccttcactt gtaaaagatt ccgttcctgc attgaatcag tattttgctg
     3301 cttttatctt tatggatctg cagtatattg gacagacagt atttaaatct ctaaacaaaa
     3361 agaaacatgc gatctttttc tcattgcttc gtaaggtatt tattgttatt ccattaactt
     3421 attttatgcc ttatgtattg cacatgggaa cttccggggt attccttgcg gaacctgttt
     3481 ctaatgtgat cggtggaggc ttttgcttca ttacgatgct ttgtactgtg ctacctgaac
     3541 ttagaagaat ggataactgt gattgatctt tttatgttaa aattttctct ccagttcatc
     3601 catctctgcc atgatctccg cttgctgttc atcaaacaaa agtcctttat tatattgata
     3661 atacttatca catccatttt ccttgataaa ccagacactg taataattaa cattcagttc
     3721 tgtcacaaat acaaccattt cctgactttg tccaaaggct tcttccatga agtcaaatgc
     3781 attgtctagt gctttggttg tattctctat ctgctgttct aattttgctg gttctttctt
     3841 gaattcttct ctcacacgtt caaattctgc ttctgcttct aaatgttcag ctttgctgat
     3901 ctgttcgtat gactccagag tcttgatcac atcaagcagc atatgttcct gtgcttttcc
     3961 aagtttctct gtctttttct tctcgttgaa gtcctcgtgg atttctttga ctacctgact
     4021 taatgtcttg cctgttttaa gttgttttag ccagccaaat aattccgtaa cataagcatc
     4081 ctgtcgataa gattcagcaa acatttcgga caattttcca tttaacaatc cgacaacact
     4141 taatttctca tcaaatgctg cgaattttaa tctgccgatc gtttcctgat tccattgtcc
     4201 ttttaaaata cgctcgattc catagtcttc tttgtatttc ttatataatt cataatagtt
     4261 ggcaaagtct ttggcgatct tcaggtgctg taagtactga aataccactt ctctgtcaat
     4321 atcttttcca agttcttcgt aaacttttaa taactgagac agatcttccc atcctctggc
     4381 tgtaacaaac gcctttccat caactgttgt ttccatacga tagaaatatt ctttacggat
     4441 ttcaagatat gcaaggattg ctgggtggat tcctgcctga tatgcgtatt ctttccacac
     4501 ttcatagtct gcttccacat caatcttctt catacggtcc aaggtaacta cgtcaaaatc
     4561 tctgacagat ttattgtatt ctggtggatt tcctgccgct acgatgatcc agccatctgg
     4621 tactttctga tttccgaaca tcttacactg caaaaactga agcattgttg gtgccaatgt
     4681 ctcagataca cagttgatct catcaataaa taaaatacct tctttatttc cagtctgttc
     4741 aatcttttca taaacagatg caatgatctc actcatcgta tattccgtaa cagaatatgt
     4801 ttgtccacta tattcttttt ctctgataaa tggcaaaccg atcgcactct gtcttgtgtg
     4861 gtgcgtgatc gtataagcta ccaatccaat ctcacattct cttgcgatct gttccatgat
     4921 ctgtgttttt ccaattcccg gagggcccat caaaaggatc ggacgctgtc tgatggatgg
     4981 tattttatat tctccataat tatcctttgt aagataagct tcaatggaat ctttgatttc
     5041 ctgttttgct cttttaatat tcatgttctt cttctccttc atcaatatct tcggcatcga
     5101 cgatcaattt aactgcccat ggcggtacat ccacatcttc gtattcttcc tgcataaaaa
     5161 taaatgctgt ctgatatggt ggtttcttct ttggataggt tccttttcca tctgtgaaat
     5221 agatcaatcc cttcagatga taaaattcac cctgttcaat cagatgattc acatattcaa
     5281 atgctggccg gaaatctgtc ccaccttctc cgatcagttc caaatgttcc atatattctt
     5341 ttaattcttt ttcatctgtg atcttctggt cgttttgtac catctcatca cactgaatga
     5401 tatgaatatt taccttatga aaaaagctct ctgcttccgt taacacacca tatgtttctt
     5461 ccagaaattt ctttaccaga tcaccagaac atgacattga tgtatcgatc acgatggcaa
     5521 agtcttcaac tttctttact tccttccact cctgcggctc gatcaatggc atatttccat
     5581 acatagaaag tccataactg taaaatatat agtcaaacga atctggatct acactgactt
     5641 cttctcttaa aaccgaaaat tttcttaaaa attcacgata atcataacgg tctctgtttt
     5701 ctacctgtac ctgtccgatc agatcccctg cttgttgtga ctcttcactg gaaaatgtct
     5761 ccatatctgt ttccgttttt tcactgatat tcttccattt attttctact tcctgatgaa
     5821 gatcttgttt gggatcttcg ggccaatact ggtggtcatc tacccgaaat tccatctgca
     5881 gttcttttat tttcttctct tctaatccaa aagaaaccaa ctgtcgataa atcccctcgg
     5941 ctgtcaatac tttgaggttc ctctcaagct gcccgtaaat gtctttgcgc agccttgttt
     6001 gcaccatatg gatacatttt aaatgccagt gatcaataat tgattcgact gcgatatcac
     6061 aagaaatatt ccatagagtc ttatctcttc cttttcttct tgtcacatga tgtaaaatgc
     6121 agtgaagcac catatgaaga tatgcacgat tcatccggac tctgttttgc ctgtacatag
     6181 ctcctggatg ataaaacata cacattccat ccgttccaac accatttaca gaaggatcga
     6241 tcacataagc aaaactgctt aatgccacat ctaaaaaacg catatggata tataattcat
     6301 ttcttgtcgt cgcaaggatc tttgttccaa gttcactgag acgctcattg atattttctt
     6361 ccattattca tttcccttta tcttatctat aattcaaatg ttttcttttt ctttggaaat
     6421 ctcttatgtt gttcgttcat caaaatactt aaaagttctg ttttctgctt tctggatgcc
     6481 cagtcgatcg ctgcttcgat tccttcttta ctgaaataat tcttctctgc catcaaattg
     6541 ataaatggca tctgatcttc ttcttttaaa taattcatca tctcgcattg gttctgcttt
     6601 acatagccct cgtattgcat ccatgcaaaa tctgataccc cgtatggcat taacatacgc
     6661 ccagctaata tctcccatac aatatccaga tcttcctgaa ctgctgccac tggaaaaata
     6721 tcatcatatt tacgatagtc gatctctcct cgcaaaaaac tttggcgata ttgatatcct
     6781 gttccatgaa aatgtgtctc cacaattctt gctggtgtat tttccacagc ctcttcataa
     6841 tactctggat aaaataaacg actttgtttt tcttcttctt tataacggat atttacctgc
     6901 aatggaaaac gagtatctct tacaacgtct cttaaagcac tttttttatc ctcataaaca
     6961 tcatagtaga tcttttgcag cttgcatcct gtaaaaattc ctgctcctgt tcttaaaaga
     7021 gaatctgaaa acccaagtgt tgtcagattt acacatccat aaaatgcata tttccctatt
     7081 tccacagtat ccggtggcag ataaacttct tttactgcct ccccacaaaa catcggttca
     7141 tcttcactgc ccaaaaagtc ttcgccgatc ataatctctt ttgcttcgtc ttcttttcgt
     7201 ttatgcgcag aaaatgcata atcaccaatc ttcgtaactc tttttccttc aatttccgaa
     7261 ggaatctcta cttgatcttc tttgctgaaa caacgaagaa cttcgattcc tccatctttt
     7321 gcatctctgt attgtatctt aagcatcgtc ttttccttcg cctttctcca tgtatacact
     7381 atccttatta tagctgactg acaggaaata tcaagaatcg taaaaaggaa tttcccatct
     7441 tcataaaaat gtaatataat gcagaaagat acgatgattt tatacactta tgatgatata
     7501 taaaggagat ttttatggaa cgactgatcg ttttaggaac tggaaatgca caggcaattc
     7561 attgctataa cacttgttat gccatgcaaa aaggtgaaga atacttcctt gtcgatgccg
     7621 gcggcggaaa tggaattcta tctcagcttg acaaagctgg aatccctctt gaaagcattc
     7681 ataatatttt tgtgacacat gctcataatg atcatatttt aggaatggtc tggatgatcc
     7741 gtatgatcgc tacttctatg aacaagggaa cttatgatgg cactatgacg atctactgtc
     7801 atgaagaact ggttcatacg atcaaaacac tgacaaaact taccgttggt aagaaatttt
     7861 ataagcattt tgatgaacgt attgtatttc atgtcattgc acatggagat acgattcaga
     7921 ttcttggaga ttcttttaca ttctttgata tcggctctac aaaagaaaaa cagtttggtt
     7981 tcacatcggt tatggaaaat ggattaaggg tatcctttcc tggagatgaa ccttatcgtg
     8041 aatctcttta tgattttgtg aaagattctg actggctgct tcatgaagca ttttgtgtgt
     8101 atggtgagag ggatattttt aaaccttatg aaaaacacca tagtacggta aaagatgcct
     8161 gtgaattagc agaacatcta aagattccaa atctggttct atggcataca gaagataaaa
     8221 atatgctgca tcgtcaagaa aaatatatga aggaaggcaa gcagtattat actggaacac
     8281 tttatatacc tgaagatctg gatgtattga aattgtaatt tggaaggtta atgatcaacc
     8341 ttccatcaaa tgataaatga tcgcagctac aacatcatag gcattttcct gcaaatgtat
     8401 tccgtccacg gtatactctc ttgacaactg cccatcttct ccgatcaatt tatcaaatac
     8461 atcaacatat tcaaagttat cttctttaca caatctttgt atttctgtat tgatcttttg
     8521 aatctcttta ttggattttc cttctgccca ggacggcatc aatccaatcc ttttggtata
     8581 tgccggatac atagattcaa tataaatctt tgtatctggc aattgctgcc gaatccttcg
     8641 tgcaagtctg atcaatcgtt ctgctaacac tcttgctcct ggatacaatg acatcaggaa
     8701 atcatttgca ccaccttgca tgataatgat atctggttta tatggaaata cgtcataagc
     8761 caatcttgca cccatctctg cgatcgtatc ccctgcaact ccactgttaa tgaactgtgt
     8821 atctatattt tttaattttt ccattgggaa atagtcggtc agggaatccc caaacataac
     8881 aattgttctc ttcttctctt ccattgtcta ctccagtact ttttgtttaa aatcctcttt
     8941 ttgctgcatg gaaatattta atcccttgat cataaactga ttaatagaac catattcttc
     9001 tttcatggtt ttaagtgctg tatttaaaaa ttcttctttt gccacaaaat agccctctat
     9061 tttttcgatt ttttcgcgat gcaatcctgc taaatgaagt ttctttttta atttttctac
     9121 tcttgtgatc gcagctttat tggtctttag ataatcttcc atcacgtcat cttcagacac
     9181 atcaagcaaa aataaaatgg ttgctgataa caatccacaa cggtcttttc cttctgtaca
     9241 atgccataaa accgcaccat tttttgtatt cataatctct ctgactgcct gtgagatctg
     9301 ctttctgcaa aattcttctt tgatcatcat cgtataaata tctgacatct ctggaaattc
     9361 ttccatatgt aacatctgtt ttcttgtttc ctgctcccgg ctgactccca ccatgctttc
     9421 cataaaaaac ggaatattct cataaacagc tcccgccact tccagatctg gctcttgttc
     9481 tacttccatt ggtgttctta aatccagaat tttctgtaaa tgatattctg tttccagtaa
     9541 aatcttatct ttctgcgtaa tccttgataa acggttgctg cggatcagct gtttatcttt
     9601 gatcactgca ccttctgtcg tttttaatcc gccaaggtct ctcgtattat ctgcaccttc
     9661 cagataaatc ttccccatgt taccgcctcc atttttgtat gatcagtttt ccttctctgg
     9721 ctgttctttc tccaaggata ttccatccat catataagtt ttctcatttt tctgattctc
     9781 cagatattct gttctgatct gtgcttttgc ttcttcatag tctctcttta attctcttgc
     9841 agaaagcaga cggcagcctt gtccgcggat aatgatctgt tccaatccat cggatgttgc
     9901 aactgttaca tcgtattttc gtccatgggt atgggcaaat ttttcaatat actgatctgc
     9961 tgtttctgct tcttttgtgt agactacatg aatattcatg taatctgtga tctctgtttc
    10021 atgtcctttc acacgatatg catcaaacac aacgatcagc tccatgccct tcattccctg
    10081 ataattactc atctcatcaa gtaattttcc tctggctgca tctacactcg tatctaataa
    10141 ttccttcaga tcatcccatg cgtaaatgat attatatcca tcaaccagca ggtatttctt
    10201 ggacatatct ctttttaccg atgatttttc tgcagatgta tgataatcct gtacaagcct
    10261 tgctgttttc ttcttcttcc atttactttt attatgctgg tttgcataaa atgtttgttc
    10321 aagaatccga tcgatttcct ctgtatcaat tgattcttct tcaatataag cccttcgctg
    10381 tgtcagttca tcaaattcct cttcctcgat ttctttcgca agaacgctct ctaaatgcat
    10441 atgttctggt acctcattcc atgcgacatt atacccggct ccatgggaac agaaaacaga
    10501 tccacatgga ttttcaagat ctctatctgg atcatatcca ctggcttcta tgatttcttc
    10561 ttcattatgg cagatatcat atccatttaa ggttgtaaat aatcttccca tacctttgga
    10621 atatgctaat acttcttttt ggtatccttg catcgtagaa actggtgcta ttccttgaag
    10681 cactgacatc tctcctgcag tatctggtgt atcaaacgta ccttgcatct tttcaatatc
    10741 tgtcatagct cttccgatca tctgctgtgg aacttccagc cggaacttat aatatggctc
    10801 taataaaaca ctctgtgcca tacgaagccc ctgtcttact gcacggtatg tcgcctgacg
    10861 gaagtctcca ccttctgtat gcttctgatg tgcccgtcct gctgccagcg tgatcttgat
    10921 atctgtgatc ggtgctcctg ttaaaacccc tctatgatct ctttctaata aatgtgttat
    10981 gatcagtctc tgccaattct tatccaatac atcttcgctg cactgtgtgt cgataacaac
    11041 tccacttccc ctctccagcg gttccatcaa caaatgtact tctgcataat gtcttaacgg
    11101 ttcaaaatgc ccaacacctt ctacaacatc tgctatcgtc tctttgtata caatattgcc
    11161 ctgtccaaat tccacatcta catcaaagcg ttctttgatc agactcttta ggatctccaa
    11221 ttgaacctct cccatgatct gcacctgaat ctcttgtaat tcttcattcc agatgatgtg
    11281 aagttctggt tcttcttctt ccagctgacg aagttttgga agtatcacgc ttgcacttgt
    11341 ctcttctggc aatatcatct ggtaagtaag tactggctcc aacacaggtt catatccgcc
    11401 ctgctctgct ccaagacact gcccagggat cgtatctgaa agtcctgtaa ccgcacagat
    11461 tcttcctgcc tgcagttcat ttactgtctc atacttttct cccgaataga tacggatctg
    11521 attgatcttt tcatcacttt ctgtaataac tgtttttacc tttaaatttc cacctgttac
    11581 tttcaaatgt gtcagacgat ttccattttc atctcttgtg atcttaaata tacgtgcacc
    11641 aaattcctct ggatattctc tttcttctgt ataaaatgca agatcactca gaaattcatt
    11701 gactccttga agttttaacg ctgatccaaa aaagcatggg aaaatcttac gttctgcgat
    11761 catttcccgg atcttggatt ccgtgatctc acctgattcc agataatgat ccagtaacgc
    11821 ctcatcttcc atagcgatca tctcataaaa ctcatcactg cccttttctc caaaatcaat
    11881 acatccgctg cttaaacgtt cctgtaattc cttcaacaat tgatcttttt gtgttccgtc
    11941 ctgatccatc ttgttaataa ataaaaatgt tgggatctta tatctctcaa gcagactcca
    12001 taatgtcaaa gtatggccct gcacaccatc tgctccactg atcacaagaa ccgcataatc
    12061 aagcacctgc agggttctct ccatctccgc agaaaaatcc acatgtcctg gtgtgtcaag
    12121 caaagtaaga tccatctcat tcattgaaac ctctgcctgc ttggaaaaaa tggtaattcc
    12181 tctggaccgt tctagttcat aagtatctaa aaatgcatct ccattatcca cacgtccgat
    12241 ttttctcaaa ttccctgcag tatataaaat tgcctctgat agtgttgtct tcccggcatc
    12301 cacatgagcc agaataccag ttactatgtg ctttttattc ttcataaatt tctttttcct
    12361 tttttcgtat tcactgtaaa taagtataac acatatcctg ttttcatgtt aaccgaatac
    12421 atccataaga ttatttgaca aatttttctt ctatgttata atcaaaaatt agtgctcttc
    12481 tttccatgta taccagaatc tggtataatg taagttaata aagagacggc atacaaacca
    12541 caaggcatac gaaaggagtt tcttatgaga gatgctttac gggatgaaat gatcaagaaa
    12601 atatgcgtca gagatacaga taatatatta gatgtcggtt gcggtgatgg taccttttta
    12661 catgaactga cacgctggaa ggatgttgaa ggatatggta tcgatgaatc tgaggataag
    12721 attcttatcg caaagcaaac ttggccagaa cttcattttg agacaggtta cagtgatttt
    12781 ctttcttttg atgataacag ctttcgtgtg atcactgtat gtgacgattt ccacaccttt
    12841 aaagatccac agaagtttgt taatgaagca tttcgtgtct tagttcctgg cggaagactt
    12901 tatattggag aatctgccct tcctgaggca ttgcgaattg tttctaatat tccatcctat
    12961 ctgacttctg gtgatgatcg cagacattct acttatgaga ctttagaata ttttaagaat
    13021 gcagggctta ccaaattccg tgtatataag aagaaacgtc tacttcttat ctctgcaaag
    13081 aaaccattag aataaattcc agaacttaaa atggagctga tacgatctgt atcagctccg
    13141 tttttcttat actttaaaca accatccaag atatttatct tttgttattt ttctgatcgt
    13201 ttttttccct aaaagtttct ccatcttttt tcttgctgta ttgttgatcc ttaccttgtc
    13261 atcttttgtt acgcatagat ctgggaacat gacacaccac cagttgtgcc cttttgctgc
    13321 tcccagatcg attcttactg catcgtaaat accttctgga aatgtatatt ggccatagtt
    13381 tttctctgga aatttttctt gtacaaaatg tacgtttacc ttttgtttca ctccctcttt
    13441 ctttaatatt tcctgtgctg tattttttat tctgtttttt tgcattttta agattttaaa
    13501 tgcttcttcc ttcgtttttg cttcactcaa ttctggactg atctgatcga tcacggcatc
    13561 tcttactttc aatttatatg tctgatccat cctgctgtta ctattagctc tcacatgaaa
    13621 tcgaatcaga ttgcttttca gattttgctg tattatgtta ttggattttc tttgtcctgc
    13681 cacaaaaatc atacatgcta tggccagaaa cagaatccct ttgatcatgt tttttctact
    13741 catattcaga ggatgtcctg tttttttctt tttatacaca acctctttct aattgtctga
    13801 aaatttatac aatatcaata acattttctt aaaattcagg gttgacgaat ctgaccagtt
    13861 atagtaacct aatatccatg aggtcatatg actgacggag cacgtggaat caaccacatg
    13921 ggagtatgat cattaatcgc cggccgtctg ggcaatcttg tctggacgtg cggcgttttt
    13981 tagtttgtta ctacattttt gacagaaaaa atatctgtca gccctaaaaa atcactcaat
    14041 ttttctcttt ttaaattttt cacttggcac gctccactgt ccagattttt actaaagata
    14101 aaaaagaatc ctgatcgttc aggattcttt tttgatctac atctcataaa taacacattc
    14161 atacggtttc atttttaacg tatttccaat gacagttgtc tgttcataat tcacaaatat
    14221 aacattttca tagtcttctg ataattcaat atctttttcc ttagaactaa agttacagat
    14281 taataccagt ttctgacatc cataagacct gctatatgca aaaatatgat cttctttttc
    14341 atatattggt ctgaaatcac catatgttag tatctcatta tattcttctg atttataaaa
    14401 tgcgatcaat cgcttgtaaa aattcaggat cgatccatat tctttttctt cttctgcgac
    14461 attcaccact ttatagttct cattaactgg catccacggt tttccttttg aaaatcctgc
    14521 attttcttcc gaactccatt gcattggtgt tctcgcattg tctctgctgt gatgtcttgc
    14581 aatctccaat gcctgttctt tggacattcc tgccttcaat gcgatctgat actgattgat
    14641 cgtttccaaa tcattaaatt catcatattt ccattgacga ttgctcatcc cgatctcttg
    14701 cccctgatat aggaaaggca gacctctctc acagaaaatg atcgttgcaa gtgctgataa
    14761 gctgtaaaat ccataatctt cttctgggat aaacagactt gcccctcttg gctgatcatg
    14821 gttctcaatg atattcgctt caaaccctgc cttggcaaca atctcttgat tatgaaaagt
    14881 ttcctctctc cattcatcaa atggctgtgg ccagtcatag ttcatataat ttttgccttt
    14941 ccgataggca tggcatggtt caaatgcaaa aatcgtagaa aaatatccat cttcaccgat
    15001 aaattgtgga agaatatctt cttttacaaa catagcctcc ccaacggtca gtgcatcata
    15061 tggttcaaag caacgatgct tcatctccat caagaaatct ccgattcctt cgacttttcc
    15121 tgtgatctta tatacatctg cgagtccatc tggaccgtct ggtttcagat cactccatgt
    15181 cagatctttc tttatattca tgatcgcatc aatccgaaag cctgaaagtc cttggtccat
    15241 ccaccaacag atcatctcat aaatcttctc acgaacagtc tcattctccc agttcagatc
    15301 tggttgttct tttgcaaaag aatgcaaata aaacttattg tcataccctg ggatcttctc
    15361 ccagacactg cctccaaaat aagatctcca gttggtcggt ggctgcccat ctttcccgtc
    15421 tttgatataa aaatatgatc catattcccc ttctggatct gccattgcct tctggaacca
    15481 ttcatgttca ctggaacaat gattcacaac aagatccatc agaatataca tatttctttt
    15541 ctttgcttcc ttgatcaatt gtttcatctc atccattgat ccaaacatcg gatcgatcgc
    15601 ataataatcg gaaatatcat aaccgttatc caccatcgga gactgataca tcggagatat
    15661 ccacaaaata tcaaccccta ggtcttttaa ataatctaac ttgctgataa ttccaggaat
    15721 gtcgccgatt ccatcaccat ttgtatcctt aaaactcttt gggtaaattt gatatgcaac
    15781 tttgttatgc caccattttt tattcatcat aattcttcta aactcccatt tgcggtttct
    15841 actaacatct tcatggcttc taccggatat ctgccgtgag ctgtttcccc cgtcagcatc
    15901 aatgcattcg ctccttgatg aactgcatga taaatatcat ttacttcagc tcttgttgga
    15961 taagggtttc ggatcataga gtctagcatc tgtgtaacta ccatgaaatt tttgttatgt
    16021 tctttgcata cctttgcaat atatgcctgc acttttggaa gttcggataa tggcatgaca
    16081 tttcctaagt ctcctcttgc tataacaata taatcacagt atggaagtaa ttcttccaaa
    16141 tgctctactc ctgtcatatt ttctattttg gcaaagattc gaatatcttc acagttgttt
    16201 tgttttagtg cttctcttag tatgatcaga tcttctttat tccgtacaaa tggcagcata
    16261 acatcagtta cttgatattg ttttgccaaa gatagattct tcagatcagc ctcagttaac
    16321 gtcggattgt tgatattaca tcctggcaaa gctatgcttt tacgtgccgt caatactcct
    16381 ccacagacaa tgcggcatct ccaggatcgt ggtgatattt tccgttctgc gatcagtttt
    16441 attttcccgt catctaataa aatttcctga tctcttctga tatgcggcat gatttcttct
    16501 ggtactaaaa ttccaccatg tcctataacc acatgtgctc ctgttgaaag gcccatatct
    16561 ccttccagat atccaattcg aagttctggc ccgatcagat cgatcattaa tttcttctct
    16621 ccatttgtct gcttcgctgc ctcaaaataa tgataaatcc atgcatggct tccttctaat
    16681 gaactatgtg ataaattcaa ccggatccct gtcattccgg catcaaacat cttctttaaa
    16741 atcgttggtt cgcaacaggt tggtcccaag gttccgtaat attccattgt gcatcacttc
    16801 ctttgtggtt attttaacat tttgaggagt ggtttccaat ggatttgatg gaaaaattct
    16861 agttctttta aatcttattt ccctacatcc catacacatc cagatcaacc ttcccatcaa
    16921 caaccacaac tccatcttct tctaacaatt tcgcctgttt tccttctcct ccaaacgcaa
    16981 atgctcctgc taattctcct tttgcattga cgactcgaaa acatggtata ttctctggat
    17041 ctggattctt atgcagggca tttcctactg ctcgtgacat ctttggattt cctgccatgg
    17101 ctgctacttg tccataggtt gccacatgcc cttttggaat ttgtttgact gcttcgtaga
    17161 ttctttttgt cggactgtcc gtgatgatat cgtagctttc tttgatcatt tttttgatgt
    17221 ctttttctgg aatggttcca tctaagatga tcgtgttcca atatttttta ttctgatggt
    17281 atcctgggat gacggcatcg taggcttctc tccagaattc tctccattct ggatctactt
    17341 tgatattcag attgatataa ccatcttttt catagaccca taagaaagct tttttacttc
    17401 cttttaccct tactaattcc caattaggat ctttgaatgg agattccaca tatgtg
//
LOCUS       DAOMUE010000012        17112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_642_length_17112_cov_2.943777, whole genome shotgun sequence.
ACCESSION   DAOMUE010000012 DAOMUE010000000
VERSION     DAOMUE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 17112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17112
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_642_length_17112_cov_2.943777"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..3723
                     /locus_tag="PLC71_01375"
     CDS             13..3723
                     /locus_tag="PLC71_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRM01327.1"
                     /translation="MKKKRQWFMCLGMIGVFTASVILSVFGQRKVKAQLNTENAGKIT
                     SSWADEKHIFSSFDDEKALSVDASYGFKMHATDQTKTIIEAGKKAAKLPEIEMPDFLK
                     KYTWFQTSQNTAGNIKIRKTNMEIYQCKADGSNGHWEKLDLVMTVTGIEKYKNQDGYV
                     AIGSGINGCAYVGIEEMTLKSDFYKAGTNTPVTIKSNMTLKDIDTYQYIGIKANKIHG
                     EYVSKNTKLSCKRSGETSIYYADFPDNYSSEDFTCVGFTFASDSFEYTFGRSLEKAPT
                     KEEQYVGYGQNMIRFDPVDPTKEVIGEDGTKTEHLRVQDLAKSWDYEVSQVIAGEIPK
                     AHYFDKFAFEDQIESCLKILDIKVYGDDEDVSSQFDISQNGNLVRAELKNPKDAEFYK
                     KSVYKLKIKVKMNIPENASKEQLDQLRKIWKEHGHYNTKETMITEKNTAKTIINDKIE
                     MTNEVRVDIELPKENVDTPGLLIRKETKQYEYQAKDEITYKVTVKNQNEKAKTAYFTI
                     QDTSFASVMDMKLQDIKINGLDKKDYILQTENNGFILKSKGDYALPYGQTIEITYTVK
                     SGVLTNGKVIDNTASAWAAGIPETKASRQVYINSPKNNVIKSAPVQLYKKGDHVTYQA
                     VITNPNPGTFMRNLEIQDELQAEGIKIIPGTVAVISGGKNIASECKITFDPSGRSYEI
                     ITPLALKNGTIPAMGSEEGKKTGSYENLRMTDKIEITYQAVIEEDGLEGQEVKNIMHV
                     AATKNSNGDLIREDKEIPSGSGMADETIKVKAPKLQITKQSDKKVYAVGETGYYKLHI
                     TQGKEGMTAQNVKVVDEFEKEGMKVQKIEVKLNEKDITSDCKIDAKDHQFTIETGKDL
                     GENDVMTVAYQVVFEKRIEGAVKNTAVAGSDNTEDDQDENTVVVKPPVLKIEKSTAHK
                     SYKEGQSGEYKIRVTQRNENMTAHQVVVEDHFEQEGMEISQIRVKYNGEDITKQCEII
                     IDENLRKFKIITGKDVSDKDELLVIYQTAFKKMITGDIKNIAESYSDDADKVRDDQVV
                     VMEAVQPALMITKKVDKTTYKVGDICEYQLVVTQTIKDAIAKNIVIEDQLSRNGAKVI
                     KNSIKIYAPDGSDITRQCTITAGENKYVIETGKSLSYDESIKVSYQVKLKEASLSGKT
                     LKNTATGKADFVKPVSAVRLVKIKKESKSTVTTGNKADRSNTSEYKRSPGTRDAASTP
                     KTGDSFDEKWIFALLAASLGGSFIYWKKKKKH"
     gene            3799..4116
                     /locus_tag="PLC71_01380"
     CDS             3799..4116
                     /locus_tag="PLC71_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01328.1"
                     /translation="MAKDFFGRLEETIQTTGKAAAQKAKEVADSAKRHNDLRVAKRDL
                     NDLYRQVGEKYFEEHMDYPQAEYIDLFNLIEKLRGDIEILKQEIDGGDDEAAEEDIED
                     IEE"
     gene            4199..4960
                     /locus_tag="PLC71_01385"
     CDS             4199..4960
                     /locus_tag="PLC71_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRM01329.1"
                     /translation="MIDTIGMTDIGNGRPSNQDAYLLRQKEKNGKLYLLAVVCDGMGG
                     LKKGEIASHYIVEVLKQWFDETLFDGLETMGIDMIEQNLIDLIRKINQNLLLMSRRME
                     ETKSMGSTLTLLFVEEHEFFVLNVGDSRTYWFDRDRKFVTTDHTLAELELRAGHLTKE
                     QAKKDPRRHILIQCVGVTKDIRIDCYKGRLNPGMEFLLCTDGFYGLLEEEEIYQVEAS
                     KEQIKEWVQKCFQKVKRRGELDNLSCVIVKVPNKK"
     gene            4882..6465
                     /locus_tag="PLC71_01390"
     CDS             4882..6465
                     /locus_tag="PLC71_01390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRM01330.1"
                     /translation="MFSESKKKRRIRQSVLCDRQSSEQEIGKIYHQRYQVRKILGEGG
                     MGKVFLALDQKTGKEVAVKIVKDQKQWDRERVILQKLKHTKGVPELFFAGKEKELFLV
                     MEYILGNSLKRYGRVCGKLYKKKSLLWMIKICKVLNKIHEQGIIHMDLKPENIMLDRS
                     GNIYLIDFGAALFAGEKLSGYGTKNYASKKQAKTEERADIYFDIYSLGKTMESLLKAT
                     DAVQKIIEKCLIDDDAKRYHNIRQIQADFERILRICRMKKGFMVVIAVFCIQNLWNQI
                     QREEQREKEVIVQKKSQREIKKAMAYFYGTDQIQKNTQLARVYLERCRGMKKNVSSYL
                     VLLDVLDDRRNDISAEKLMKIVQDCQTDVHDFWSAYFYLHFYTVNTAKLSENVWKEAE
                     KMLEQIQKYPQKEEYQKLVEADRINLYEREAEKGDDRKFLEETERVFKENLRGDPAWK
                     LYERKLVYLESKQPDISREFERFLKKYPKVMDAYVEYSIYLCRNNKMTQAQRVYREGR
                     KQTGMTSKRAEELRRKLGL"
     gene            6462..8567
                     /locus_tag="PLC71_01395"
     CDS             6462..8567
                     /locus_tag="PLC71_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01331.1"
                     /translation="MIRKYKIYIIMGITVLLLFMVLPVDTKGDWEIPWDITLEGEGND
                     PAFRSSTVMEIFLKNGTAPKDITVFVNQQKINPIWDYEQSTQISFQEEGIYKVHIVHK
                     NGYEEIRQVMVELNNPTTAKITAGAYTPGAWSKKNVVLEAYGAKAVSDIQFYEYKIGQ
                     DEWKQMKNNKLEISKDFDELVYIRAVSKAGREGVISQIPVRVWKQKPELAKIQCDQEP
                     IGGWYSKIPVFSYDLKEKEGPQVHLYAQLTDLKTKEILTGIDQIPRIRKDGRYQLDIY
                     TKDESGNQSEQLYQTTCFVDTDNPEIFVRYQNPRSEILKYQKAQIIVKDENLKKGNMI
                     LRTSGKQVKPWKLEGDHYETEVIFLKDGKQTLSVQAEDLAGNKMIIQEKSFIIDTQKP
                     RIKIQGIDNGRSYSKPVKIQVDVKDQNLNPDKTYIYLNGKKMDSVMIKKDGYYTIDVK
                     TEDLAGNQNRCSRKFTVNQKGIQIHFLQEDLKEKQISTKNLKPGFRIESLEPVQVMAF
                     LVNGQKKEYQWKEDKVYIKDPITENGKCSVSLHVKDSAGNEKTSEEIQFFYDTQKPVI
                     KIEGLDQKSECEYGKQISILLENKTDQWEKVILDGKEQKLEHHKITWKELAPGKHVLH
                     LKAVDQAGNKTDQRITFKITKVLPKAVKQIVVKQDKIPSKKDEKNQKHPNLWLLFLIG
                     TSIFVSVKMFCSYRKSRHS"
     gene            8635..9333
                     /locus_tag="PLC71_01400"
     CDS             8635..9333
                     /locus_tag="PLC71_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRM01332.1"
                     /translation="MAEEVKETREDFEAQLEESLNKASTNEDAVWNRLEQMKEDGDVL
                     ALTVGGVVNGGVIVYVEGIRGFIPASLLSTKYVEDLNVWLQKDVEAKIITVEPEEQRL
                     VLSAKAVEKEKERKERENKINELKVGTVVEGTVENIMPYGAFVDIGDGISGLVHISQL
                     SQKRVKSPEEVVKEGDAVTVKIIKVANGKVSLSIKALEDDKEAAKKAEETGVEYTEDA
                     AGTSLGDLLANIKL"
     gene            9346..10446
                     /gene="aroC"
                     /locus_tag="PLC71_01405"
     CDS             9346..10446
                     /gene="aroC"
                     /locus_tag="PLC71_01405"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRM01333.1"
                     /translation="MAGSGFGTNFRITTWGETHGAGVGVVVDGCPAGLPLEREDVQKY
                     LDRRKPGQSKYTTQRKEGDQVEILSGIFEGRTTGTPISMVVYNKDQRSKDYSNIKDIY
                     RPGHADFTFDMKYGFRDYRGGGRSSGRETIGRVAAGAVAAKVLKELGIEIKAYAKAIA
                     GIEVSKDHFDFAEMNNNPFNMPDKEAAKLVQEKADQMIKEMDSIGGIIECQITGMPVG
                     IGETVFDKLDAELSKAIMSIGSVKGFEIGDGFEAANLRGSENNDDFICVDGKVAKETN
                     HSGGVLGGMSDGSAIVFRAAIKPTPSIAQTQKTVNRDLDNVEIEIHGRHDPMIVPRAV
                     VVVESMAAVTILDGVLKNLGATMDNIKKIYGE"
     gene            10449..11576
                     /gene="tgt"
                     /locus_tag="PLC71_01410"
     CDS             10449..11576
                     /gene="tgt"
                     /locus_tag="PLC71_01410"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRM01334.1"
                     /translation="MYELIKKDGRAKRGRLTTVHGVIETPVFMNVGTVAAIKGAVSTD
                     DLKDIGTQVELSNTYHLHVRTGDKLIKEFGGLHKFMHWDRPILTDSGGFQVFSLAKLR
                     KIKEEGVSFASHIDGRKIFMGPEESMQIQSNLGSTIAMAFDECPPHPATREYMQNSVD
                     RTTRWLRRCKKEMDRLNSLEDTVNPHQMLFGINQGGTFEDIRIEHAKEISKMDLDGYA
                     LGGLAVGETHEEMYNILEKTVPYLPEDKPTYLMGVGTPVNILEAVDRGVDFFDCVYPT
                     RNGRHGHVYTNQGKLNMFNKKYELDHRPIEEGCQCPACRSYSRAYIRHLLKAKEMLGM
                     RLCVLHNLYFYNTMMSEIRQAIEEGNYKEYKKRKIDGMMNQ"
     gene            11603..11896
                     /gene="yajC"
                     /locus_tag="PLC71_01415"
     CDS             11603..11896
                     /gene="yajC"
                     /locus_tag="PLC71_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRM01335.1"
                     /translation="MDQVTMTILMLVVCFGLMYFLTIRPQKKKEEELKQMMETLEVGD
                     SVLTTSGFYGVVVDKVDETTIIVEFGNDKHCRIPMEKSAIQAMEKANASSSEE"
     gene            complement(11976..12803)
                     /locus_tag="PLC71_01420"
     CDS             complement(11976..12803)
                     /locus_tag="PLC71_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRM01336.1"
                     /translation="MIDLHIHSTCSDGTFTPKQIVQKVIAKGLYGFSLTDHDTVDGIP
                     EILAMDLPDDLKFIPGIEISCDALHREIHVLGYGINYKDQQLNHTLNMLRDKRFQRNL
                     DMIELFQKDGYPITLEKLQNGDPHTVITRAHFARTLISEGICSSMDQAFSKYLGEKCK
                     YYVPKPFFDPKDCLRLILDAGGIPVLAHPFLYKFSNEDTKHLIHDLKEEGLAGIEVYH
                     SSHHIGQITKLRQWQKEYDLLATGGSDFHGTNKPDIEIGTGRGPLFVPDHLIDDLMN"
     gene            12901..13266
                     /locus_tag="PLC71_01425"
     CDS             12901..13266
                     /locus_tag="PLC71_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HRM01337.1"
                     /translation="MDMGKKGLYMIQSLVFAYIATGILLCLLAFIIYQSNAGMKIANL
                     GITLTYILASVFAGMLIGSKIGKKKFLWGFAAGMLYFMILTLISWIFQKNIVLFSTER
                     ITACFLCIAGGTLGGMLAI"
     gene            13323..13466
                     /gene="scfA"
                     /locus_tag="PLC71_01430"
     CDS             13323..13466
                     /gene="scfA"
                     /locus_tag="PLC71_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HRM01338.1"
                     /translation="MKHIKTLTNKTLQNTVKKGGCGECQTSCQSACKTSCTVGNQTCE
                     HSK"
     gene            13562..14938
                     /gene="scfB"
                     /locus_tag="PLC71_01435"
     CDS             13562..14938
                     /gene="scfB"
                     /locus_tag="PLC71_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HRM01339.1"
                     /translation="MVHQYKNNGYNMVLDVASGSVHVVDDLTYEVIALYEDHTLEEII
                     KELSSWDEKDVKESYGEVTELKDAGVLFTEDCYEEYICGFKDRPTVVKALCLHIAHDC
                     NLACRYCFAEEGEYKGRRALMSAEVGKKALDFLVENSGNRRNLEVDFFGGEPLMNFDV
                     VKEIVAYGRSLEETHDKKFRFTLTTNGVLLNDDIMEFANKEMDNVVLSVDGRKEVHDY
                     MRPFRNGKGSYDFIIDKFKKFADSRNQQKYYVRGTFTHHNLDFSKDVMHLADAGFEQI
                     SVEPVVAPDTADYAITEEDLPILMDEYDLLAKEMIKREKEGKGFTFFHFMIDLTGGPC
                     VAKRLSGCGSGTEYLAVTPWGDLYPCHQFVGEEDFLMGNVYEGVKKDEIVKEFKACNV
                     YSKEECKKCFARFYCSGGCSANSYNFNGTIDGTYEIGCKLERKRVECALMVKAALALE
                     GEKENEEA"
     gene            14925..>17112
                     /gene="secD"
                     /locus_tag="PLC71_01440"
     CDS             14925..>17112
                     /gene="secD"
                     /locus_tag="PLC71_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRM01340.1"
                     /translation="MKKRNSSGKYIAGLILLIALCVFGAFITTKGITKKKIGRASNIE
                     LGLDLAGGVSITYEVDGKNVSDSDMKDTVYKLQKRVEGYSTEAEVYQEGKDRINIEIP
                     GVTDANKILDELGKPGTLSFAVQKQVKVKKNGKTTTQTTLDTVLTGQNVKKAKAVTNQ
                     NQTTGKKEYLVALEFDAKGTKAFAKATKANIGKPIYIIYDNQVISAPNVQEAITKGEC
                     TIDGMESYEAAENLASSIRIGSLPVKLNELRSNVVSAKLGVNAIQTTVKAGVIGFVLV
                     CLIMIAFYAVPGMIACVALAIYMLMMLLALNGFNATLTLPGLAGIILGIGMAVDANVI
                     IYARIQEEIGAGKSTGAAIKSGFGKAASAIIDGNVTTLIAVLVLWFKGSGTVKGFAQT
                     LGMSVLISMFTALVISRFLVYAAYHFGLRDKKWYGKPRTIKTRDFVRTGKKYVAVAGV
                     IILIGLAALPMNRSKIGSILNYDLEFSGGTASTITFKDDQKVNDSLEKQVVKAYEKVS
                     KSTSVQSQKVKANNQMVVKSVELNLSQRKQIESTLKKDYKVKSVTTENISSTISNEMQ
                     RDAFISVVISAICMLIYIAIRFKDVKFGSSAIIALINDVLVVFAAYSVGRLSVGGTFI
                     ACMLTIIGYSINSTIVIFDRIRENLKLQTIRTRDDIKALVNQSISSTLVRTINTSLTT
                     FVMVLALFICGVSSLREFALALMVGVIGGAFSSVFLTGPLWYMMKTR"
BASE COUNT         6249 a         2494 c         3975 g         4394 t
ORIGIN      
        1 ggagagtaca ttatgaagaa aaagagacaa tggttcatgt gccttgggat gatcggagtt
       61 tttacggcat ccgtaatctt atccgtgttt ggacagcgaa aggtgaaagc acagttaaat
      121 actgagaatg cggggaagat cacaagcagt tgggcagatg agaaacatat tttctcatca
      181 tttgacgatg aaaaagcact ttcagtggat gcatcctacg gttttaagat gcatgcaaca
      241 gatcagacga agacgatcat agaagcagga aagaaagcag caaaattacc agagatcgag
      301 atgccggatt tccttaaaaa gtatacatgg tttcagactt cacaaaatac cgcagggaat
      361 attaagatcc gaaaaacaaa tatggagatc tatcaatgca aagccgatgg aagtaatggc
      421 cattgggaaa aattagattt ggtcatgacg gttactggaa tcgaaaagta taagaatcaa
      481 gacggttatg tggcgattgg aagcggcatc aatggatgtg cttatgttgg aatcgaagag
      541 atgacattaa aaagtgattt ttataaagca ggaacgaata caccagtaac gatcaaaagt
      601 aatatgacgt taaaggatat tgatacatat cagtatatcg ggatcaaagc aaacaagatt
      661 cacggagaat atgtatcaaa gaatacaaaa ttaagttgta aaagatcagg ggaaactagt
      721 atttattatg cggattttcc agacaattac agtagtgaag attttacttg tgtaggattt
      781 acatttgcat cagattcttt tgaatataca ttcggaagga gtcttgaaaa agcaccaaca
      841 aaagaggagc aatatgtagg atatggacag aatatgatcc gctttgaccc agttgaccca
      901 accaaagagg tgatcggaga agatgggacc aagacggaac atctaagagt gcaagatctg
      961 gcaaaatctt gggattatga agtcagtcag gtaattgcgg gagaaattcc aaaggcacat
     1021 tattttgata agtttgcatt tgaagatcag atcgaatcct gtttaaagat tctggatatc
     1081 aaggtttacg gagatgatga ggatgtaagc agtcagtttg atatttcaca aaacggcaat
     1141 cttgtaagag cagaacttaa gaatccgaaa gatgccgaat tttataaaaa atctgtttat
     1201 aaattaaaga tcaaagtaaa gatgaatatt ccggagaatg catccaaaga acagttagat
     1261 caattgcgta agatatggaa agaacatggg cattataata caaaggaaac gatgatcaca
     1321 gaaaaaaata cagcgaaaac gatcattaat gataagatag aaatgaccaa tgaggttcgt
     1381 gtagatatcg agctgccaaa agaaaatgta gatacgccgg gtcttttgat caggaaagag
     1441 acaaaacaat atgaatatca ggcaaaagat gagatcacct ataaggtaac agtgaaaaat
     1501 caaaacgaaa aagcaaagac agcttatttt acgattcagg atacatcctt tgcaagtgtg
     1561 atggatatga agttacagga tataaaaatc aatggtcttg ataagaagga ttacatatta
     1621 cagacagaaa ataatggatt tattttgaag tcaaaaggtg attatgccct tccatatgga
     1681 cagacgatcg agatcacata tacagtaaaa agtggagtcc ttacaaatgg aaaagtgatc
     1741 gataatactg catctgcatg ggcagcagga attccagaaa caaaggcatc aaggcaggtg
     1801 tatatcaact cgccaaaaaa taatgtgata aaatcagctc cagtacagct ttataaaaaa
     1861 ggagatcatg taacttatca ggcagtgatc accaatccaa atccgggaac ttttatgaga
     1921 aatttggaga ttcaggatga acttcaggca gaagggatca agatcatacc aggaaccgta
     1981 gcggttatat ctggtgggaa aaacattgca tctgagtgta agatcacatt tgatccaagt
     2041 ggaagaagtt atgagatcat aacaccactt gcactgaaaa atggtacaat tccagcaatg
     2101 ggttcggaag aaggaaagaa aacaggatct tatgaaaatc ttcgcatgac ggataagata
     2161 gagatcactt atcaggcagt gatcgaagaa gatggactcg aaggacagga agtaaagaat
     2221 attatgcacg ttgctgcgac aaaaaacagc aatggagatc tgatccggga agataaagag
     2281 attccgtcag gaagcgggat ggcagatgag acgatcaagg tgaaagcacc aaaactgcaa
     2341 atcacaaaac aatcggataa aaaggtttat gcagtaggag aaactggata ttataaactt
     2401 catatcacac aaggaaaaga aggcatgaca gcacagaatg taaaagtcgt ggatgagttc
     2461 gaaaaagaag gtatgaaggt tcagaagatc gaagtaaaac ttaatgaaaa agatatcaca
     2521 tcggattgca agattgatgc gaaagaccat cagtttacga ttgaaacagg aaaagatctt
     2581 ggtgaaaatg atgtgatgac ggtagcttat caggttgtat ttgaaaagag gattgaagga
     2641 gccgtgaaga atacagcagt tgcaggaagt gataatacag aagatgatca ggatgaaaat
     2701 acagtcgttg taaaaccacc tgtgttaaag attgaaaaaa gtactgcgca taagtcttac
     2761 aaagaaggac aaagcggaga atacaagatc cgtgtgacac agagaaatga gaatatgaca
     2821 gcacatcagg tagtcgtaga agatcatttt gaacaagaag gaatggagat ttcacagatc
     2881 cgtgtaaaat acaatgggga agatatcacc aagcaatgtg agatcataat agatgagaat
     2941 cttagaaaat ttaagatcat cacaggaaaa gatgtcagtg acaaagatga acttctggta
     3001 atatatcaga cagcatttaa gaagatgatc acaggagata tcaaaaacat tgcggaaagc
     3061 tatagtgatg atgcggataa agtcagagat gatcaagtgg tagtaatgga agctgtacag
     3121 ccagcattga tgatcaccaa gaaggttgat aaaacaacat ataaagttgg tgatatctgc
     3181 gagtatcagc tggttgttac gcagacgatc aaagatgcga tcgcaaaaaa tattgtgatc
     3241 gaagatcagc tttcaagaaa tggagctaag gtgatcaaga acagcattaa gatttatgca
     3301 ccagatgggt cagatattac cagacagtgc acgatcactg caggagaaaa taagtatgtc
     3361 attgagacag gaaagagttt atcttatgat gaatccataa aagtatcgta tcaggtaaaa
     3421 ctaaaagaag catcactttc tggaaagaca ttaaaaaata cagcgacagg aaaagcagat
     3481 tttgtcaaac cagtcagtgc agtacgttta gtgaagatca aaaaagaatc gaaatcaaca
     3541 gtgacaacgg gaaataaagc agatcgatcc aatacttctg aatataaaag atcaccagga
     3601 acaagggatg cagcgagcac accaaaaaca ggagattcct tcgatgagaa atggattttt
     3661 gcattgttgg cagcatcttt aggtggaagt tttatatatt ggaaaaagaa gaaaaaacac
     3721 tagtaattct agggacaaag cctttacgaa acactgagga taaggtacaa tgaaagaaaa
     3781 atgttgggag tgacgtatat ggcaaaagat tttttcggac gtctggaaga aacgattcag
     3841 accacaggga aagcagcggc gcagaaagct aaagaagtcg cagattccgc aaaaagacac
     3901 aatgatctaa gagtcgccaa gagagattta aatgatctgt accgtcaggt gggagagaag
     3961 tattttgaag aacatatgga ttacccacag gcagagtata ttgacctgtt taatctgatt
     4021 gagaagcttc gtggagatat cgaaatcctg aagcaggaga ttgacggagg agacgacgaa
     4081 gcggcggaag aagatatcga agatatcgag gaataaacta ttaacaaaca caacaaaaca
     4141 gtgaaagaag cttgtaaaga tttatcttta tgagcttttt ttcatttagg aggaatttat
     4201 gatagataca attggaatga cagatattgg gaatgggaga ccatccaatc aggatgctta
     4261 tctgttgcgt cagaaggaaa agaacggaaa gctttatctg ctcgcagtcg tctgtgacgg
     4321 catgggaggt ttgaaaaaag gagagattgc cagtcattat atcgtggaag tattaaaaca
     4381 atggtttgat gaaacgttgt ttgatggatt agaaactatg ggaattgata tgatcgagca
     4441 gaatctgatc gatctgatcc gcaagatcaa ccagaatctt ttgttaatga gtcgtcggat
     4501 ggaagaaaca aaatccatgg gaagcacatt aaccttattg tttgttgaag aacatgaatt
     4561 ttttgtctta aatgtcggtg attcgagaac ttactggttt gacagggacc gtaaatttgt
     4621 aacgacagat cacactctgg cagagttgga actgcgtgct ggacatctca caaaagaaca
     4681 ggcaaaaaaa gatccgagaa gacatatttt gattcagtgt gtaggtgtga caaaagatat
     4741 ccggattgac tgttacaaag gcagattaaa tccaggaatg gagtttttat tatgtacgga
     4801 tggcttttat gggctgcttg aggaagaaga aatctatcag gtagaggcat caaaagaaca
     4861 gatcaaagaa tgggtgcaga aatgttttca gaaagtaaaa agaagaggag aattagacaa
     4921 tctgtcctgt gtgatcgtca aagttccgaa caagaaatag gaaagattta tcaccaacgt
     4981 tatcaagtcc gaaaaatact tggagaaggc ggaatgggta aagtttttct tgcactggat
     5041 caaaagacag gaaaagaggt cgctgtgaag atcgtaaagg atcaaaaaca gtgggacagg
     5101 gagagggtga tcttgcaaaa attaaaacat accaaaggtg tgccggaatt attttttgca
     5161 ggaaaggaaa aggaactttt tctggtgatg gaatatattc ttggaaattc gttaaaacga
     5221 tatggcagag tctgtggaaa actttataaa aagaaaagtt tattgtggat gatcaaaatc
     5281 tgcaaagtgc tgaataagat tcatgaacaa ggaatcattc atatggatct gaaaccggaa
     5341 aatatcatgc ttgatcgatc tggaaatata tacttgatcg attttggagc ggcattgttt
     5401 gcgggagaaa aattaagtgg atatggaacc aaaaactatg catcaaaaaa acaggcaaag
     5461 acagaagaaa gagcagacat ttattttgat atatatagtt taggaaaaac aatggaatct
     5521 ttattgaaag caacagatgc tgtccaaaag atcatagaaa aatgtctgat cgatgacgat
     5581 gcaaaaagat atcacaacat aaggcagatt caggcagatt ttgagagaat attgagaatc
     5641 tgtcgtatga agaaagggtt catggtggtg attgctgttt tttgtatcca gaatctctgg
     5701 aatcagatac agagggaaga gcaaagagag aaagaagtca ttgtacagaa aaagtctcaa
     5761 agagagataa aaaaggcaat ggcttatttt tatggaacgg atcagataca aaagaataca
     5821 caattggcac gagtgtatct tgaaagatgt agagggatga aaaagaatgt gtcatcgtat
     5881 ttggtgcttt tagatgtttt agatgacagg agaaatgata taagtgcgga aaaacttatg
     5941 aagatcgtac aggattgtca gaccgatgtg catgatttct ggtcggcata tttttatctt
     6001 catttttata cagtaaatac agcaaaactg tcagagaatg tatggaaaga agcagaaaaa
     6061 atgttagaac agatacaaaa atatccacaa aaggaagaat atcagaaatt agtggaagca
     6121 gaccggatca atttatatga aagagaagca gaaaaaggag atgatagaaa gtttttggag
     6181 gaaacagagc gggttttcaa agaaaatctt agaggagatc ctgcatggaa attgtatgaa
     6241 agaaagcttg tttatctgga atcaaaacag ccagatatca gcagggagtt tgaacgattt
     6301 ctaaagaaat atccaaaagt tatggatgcc tatgtggagt atagtattta tttatgcagg
     6361 aacaataaaa tgacacaggc acaaagggtc tatagggaag gtagaaaaca gacaggaatg
     6421 acaagcaaaa gagcagagga attgaggagg aaacttgggt tatgatcaga aaatataaga
     6481 tatatatcat catgggaatc acagtacttc ttctttttat ggttttaccg gtcgatacga
     6541 agggagattg ggaaatccct tgggatatta ctctggaagg agaaggtaat gatcctgcat
     6601 ttcgaagcag tacagtgatg gaaatatttc taaaaaatgg gacagcacca aaggatatta
     6661 cagttttcgt gaatcaacag aaaataaatc cgatctggga ttacgaacaa agtacacaga
     6721 tcagttttca ggaagaaggg atttataagg ttcatattgt tcataaaaat ggatatgaag
     6781 aaataaggca ggttatggtc gaattaaata atccaacaac agccaagatc acagcgggag
     6841 cgtatactcc aggagcatgg agcaaaaaga atgtggtatt ggaagcttat ggagccaaag
     6901 cagtttctga tattcaattt tatgaatata aaataggaca agatgaatgg aagcagatga
     6961 aaaataacaa gttagagatc agcaaagatt ttgatgagct tgtttatatc agagcagttt
     7021 caaaggctgg aagagaagga gtgatcagtc agattccagt gagagtatgg aaacaaaaac
     7081 cagaacttgc aaagatccaa tgtgatcagg aacctattgg tggatggtat tcgaaaattc
     7141 ctgttttctc atatgatctg aaggaaaaag aaggaccaca ggtgcatctt tatgcgcagc
     7201 ttacagattt gaagacaaaa gagatactga caggaatcga tcagattcct aggatcagga
     7261 aggatggaag atatcagctt gacatctata cgaaagatga atctggaaat caatcagaac
     7321 aactctacca aacgacttgt tttgtggata cagataatcc agagattttt gttcgttatc
     7381 aaaatccaag atcagaaatt ttaaaatacc agaaagctca gatcatagtt aaggatgaaa
     7441 atctgaaaaa gggaaatatg atcttaagaa catcggggaa acaggtaaag ccatggaagt
     7501 tggaaggtga tcattatgaa acagaagtta tatttttaaa ggacggaaaa cagacattgt
     7561 ctgttcaggc agaagatctg gcaggaaata agatgatcat acaggaaaag tcgtttatca
     7621 ttgatacaca aaaaccaagg attaaaattc aaggaattga caatggaaga tcatattcaa
     7681 agcctgtaaa aattcaagta gatgtgaaag atcaaaatct gaatccagat aagacatata
     7741 tttatcttaa cggaaagaag atggattccg ttatgatcaa aaaggatgga tattatacca
     7801 ttgatgtgaa aactgaagat cttgcaggaa atcaaaatag gtgcagcaga aaatttactg
     7861 tcaatcaaaa aggcatacag attcattttc tgcaggagga tttaaaggaa aaacagatat
     7921 caacaaaaaa tctgaagcca ggatttcgca ttgaaagtct ggaaccagtg caggtaatgg
     7981 catttcttgt gaatgggcaa aaaaaggaat atcaatggaa agaagataaa gtttatataa
     8041 aagatcctat cacagaaaat gggaaatgca gtgtcagtct gcatgtaaaa gacagtgccg
     8101 gaaatgaaaa aacaagtgaa gaaatacagt ttttttatga cacacaaaaa ccggtgatca
     8161 aaatagaagg gcttgatcag aaatctgaat gtgaatatgg aaaacagatc agcatccttc
     8221 tggaaaataa aacagatcag tgggagaagg ttattttgga tggaaaagaa caaaaactgg
     8281 aacatcataa gattacatgg aaagaattgg ctcctggaaa gcatgttctt catctgaaag
     8341 cagtggatca ggcaggaaat aaaactgatc aaaggatcac atttaagatc acaaaagtcc
     8401 ttccaaaagc agtgaaacaa attgtggtaa aacaagataa aataccatca aaaaaagatg
     8461 aaaaaaacca aaaacatccc aatttatggc ttttatttct cataggaaca agcattttcg
     8521 tatctgtaaa aatgttttgt agttatagga aatccaggca ttcttgaatc tttttcattc
     8581 tatgttataa taaaggttac gtatatagtt caatcaaatg gaggaatgga tattatggca
     8641 gaagaagtaa aagaaacaag agaagatttc gaagcacagt tagaagaatc tttaaataaa
     8701 gcatcaacaa atgaagacgc agtatggaac cgtttagagc agatgaaaga agatggggat
     8761 gttctggcat tgacagttgg cggtgttgta aatggtggag ttatcgttta tgtcgaagga
     8821 atccgtggat ttattccagc atctttatta tcaacaaaat atgtagaaga tctgaacgta
     8881 tggttacaga aagatgtgga agcaaagatc atcacagtag aaccagaaga acagagactt
     8941 gtcttatctg caaaagctgt tgagaaggaa aaagaaagaa aagaacgcga gaataagatc
     9001 aatgaattaa aagttggtac agttgtagaa ggtacagttg aaaatatcat gccatatgga
     9061 gcatttgttg atatcggtga tggaatcagt ggattagttc atatttcaca gttaagccag
     9121 aagagagtaa aatctccaga agaagttgtg aaagaaggag atgctgttac agttaagatc
     9181 attaaagtcg caaatggaaa agtaagttta agtatcaaag cattagaaga tgataaagaa
     9241 gcagctaaga aagcagaaga aactggtgtg gaatacacag aagatgcagc tggaacaagt
     9301 cttggtgact tacttgcaaa tatcaaatta taggaaggaa catatatggc aggatcaggt
     9361 tttggcacaa attttcgaat tacaacatgg ggggaaactc atggtgcagg cgtaggtgtc
     9421 gtggtagatg gatgtccggc tggattacct ctggagagag aagatgttca gaaatatctg
     9481 gaccgcagaa aaccagggca gtcaaagtat acaacacaga gaaaagaagg agatcaggtt
     9541 gagattcttt ctggtatctt cgaaggaaga acaacaggga ctccgatctc catggttgta
     9601 tataacaagg atcagaggtc taaagattac agcaatatca aagatattta ccgtccagga
     9661 catgcagatt ttacatttga catgaaatat ggattcagag attaccgcgg tggcggcaga
     9721 tcttctggac gagagacgat cggacgtgta gcagcaggag ctgtagcagc gaaagttttg
     9781 aaagaactgg gaattgagat caaagcttat gcaaaagcga tcgccggaat tgaagtatct
     9841 aaggatcatt ttgactttgc ggagatgaat aacaatccat ttaacatgcc agataaagag
     9901 gcagcaaaac ttgtacagga gaaagcagac cagatgatca aagagatgga ttccatcggt
     9961 ggtatcattg aatgtcagat cacaggaatg ccagttggaa tcggggaaac cgtttttgat
    10021 aaactggatg cagaactttc gaaagccatt atgtccattg gatcagtaaa aggcttcgag
    10081 atcggagatg gatttgaggc tgccaacctg cgagggtcag agaataatga tgattttatc
    10141 tgtgttgatg ggaaggtagc aaaagagaca aatcattctg gaggagttct tggaggaatg
    10201 agtgatggaa gtgcgatcgt attccgtgca gccatcaaac cgacaccatc gatcgcacag
    10261 acacagaaga cagtcaatcg tgatcttgat aatgtagaga ttgaaattca tggccgtcat
    10321 gatccaatga tcgtgccaag agctgtagtt gtggtagaat caatggcagc tgttacgatt
    10381 cttgatggag ttttaaagaa tcttggtgca acaatggata atattaagaa aatttatggt
    10441 gaataagaat gtatgaatta atcaaaaaag acggaagagc aaaaagaggc cgtttaacaa
    10501 cggtccatgg tgtgatcgaa acaccagtat ttatgaatgt cgggactgtg gcagcgatca
    10561 aaggtgcagt cagtacagat gatctcaaag atattggaac acaggtggag ttatctaata
    10621 catatcatct gcatgtaaga acaggagata agctgatcaa ggaatttgga gggctgcata
    10681 agtttatgca ctgggaccgc ccaatcctta cagattctgg cggatttcag gtgttttcat
    10741 tggcgaagct tcgtaagatc aaagaagaag gcgtttcttt tgcttctcat attgatggaa
    10801 gaaagatttt catgggacca gaagaaagta tgcagatcca gtcaaactta ggatcaacga
    10861 tcgcgatggc atttgacgaa tgtccgccac atcctgcaac aagagagtat atgcagaatt
    10921 ctgttgatcg tacaacaaga tggttgagac gatgcaaaaa agagatggac cgcttaaatt
    10981 ctctggaaga tacagtcaat ccacatcaga tgttatttgg gatcaatcag ggtggaacat
    11041 ttgaagatat ccgaatcgaa cacgccaaag agatttccaa gatggatcta gatggatatg
    11101 cattaggtgg acttgcggtt ggagaaacac atgaagagat gtacaatatc cttgaaaaga
    11161 cagtgccata tctgccagaa gataaaccaa cttacctgat gggggttgga acaccggtga
    11221 atattttaga agcagttgac cgtggagttg atttctttga ttgtgtatat ccaacaagaa
    11281 atggccgtca tggacatgta tatacgaatc agggaaaact taatatgttc aataaaaaat
    11341 acgaactgga ccatcgcccg atcgaagaag gatgccagtg cccagcctgt agaagttaca
    11401 gcagagccta tatccgacat cttttaaaag caaaagaaat gctaggaatg agattatgtg
    11461 tcttgcataa cttgtatttt tataatacaa tgatgagtga gattcgtcag gcaatcgaag
    11521 aaggcaatta taaggaatac aagaaacgta agatcgatgg catgatgaac cagtaatctt
    11581 attagaaaga tggaggaaga aaatggatca ggttacaatg acaattctga tgttggttgt
    11641 ttgttttgga ttaatgtact ttttaacgat cagaccacag aagaaaaaag aagaagaatt
    11701 aaaacagatg atggaaactc tggaagttgg agacagcgtg ttgacaacaa gcggatttta
    11761 cggagttgtt gtagataaag ttgatgaaac aacaatcatt gttgaatttg gtaacgacaa
    11821 acattgccgt attccaatgg agaaatcagc aatccaggca atggaaaaag cgaacgcatc
    11881 atcatctgaa gaataagatg aatatttgat caaaagaaaa gaatctgaga cagagaatag
    11941 tatctgtatt tcagattctt ttttgtttct taatattaat tcatcagatc atcaatcaaa
    12001 tgatctggga caaataacgg tccgcgtccg gttccgattt caatatctgg tttattcgtt
    12061 ccatggaagt ctgatcctcc agttgcaaga agatcatatt ctttctgcca ttggcgaagt
    12121 tttgtgatct ggccgatatg atgggaagaa tgataaactt cgattcctgc gagcccttct
    12181 tctttcagat catgaatgag atgtttggta tcttcatttg agaatttata taaaaatgga
    12241 tgtgcaagaa ccggaattcc gccagcatca aggattaatc ttaagcaatc ttttggatca
    12301 aaaaatggtt ttgggacata atatttacac ttttcaccaa gatatttgga aaaagcctga
    12361 tccatggaag aacaaatgcc ttctgagatc aaagttcttg caaagtgagc tcgtgtgatc
    12421 acagtatgag gatcaccatt ttgcagtttt tcaagcgtga tcggataacc gtctttttga
    12481 aagagttcga tcatatcaag atttctctga aaccgtttgt cacggagcat gttgagagta
    12541 tgatttaact gctgatcctt atagttgatt ccgtatccta atacatgaat ctcacgatga
    12601 agtgcatcac atgaaatttc gattccagga atgaatttca gatcatctgg aagatccata
    12661 gcaagaattt ccggaattcc atcgacagta tcatgatctg tcagagaaaa tccatataat
    12721 ccttttgcta tcactttttg aacaatctgt tttggtgtaa aagttccatc ggaacaagta
    12781 gaatgtatat gaagatcgat cataggaaaa ctcctttcgt atttttaatg tcagtttatc
    12841 acaaaaaggc atagattcaa aataccagtc atagattaaa tagaaaaagg agcaattgtt
    12901 atggacatgg ggaaaaaggg actctatatg atacaaagtc tggtatttgc ctacattgcc
    12961 acaggaattt tattgtgtct tcttgcattt atcatctatc aaagcaatgc aggaatgaag
    13021 atcgcgaatc ttggaatcac acttacatat attcttgcat ctgtttttgc tggcatgctg
    13081 atcggaagta agatcggaaa aaagaagttt ttatggggat ttgcagcagg aatgttatac
    13141 tttatgatct tgactttgat ctcgtggatt tttcaaaaaa atatcgttct gttttccaca
    13201 gaaaggatca cagcatgttt cttatgtatt gcaggaggaa ctttaggtgg aatgcttgct
    13261 atttgaaaat gaatatgata caatatagca agttatttta tgagaatcag gaggacgata
    13321 agatgaaaca tattaaaact ctgacaaata aaacattaca gaacacagtg aaaaaaggtg
    13381 gatgtggtga atgccagaca tcttgccagt cagcatgtaa aacatcttgt acagtaggta
    13441 accagacctg cgaacacagc aaatagtcaa atcgaacaaa gactttgtgc ttgaggcgtt
    13501 ccttgtgggc cgcctcattt tttcgatgaa agaaaataac agataaaggg agaaagaaac
    13561 aatggttcac cagtataaaa ataatggata taacatggtc cttgatgtag caagtggatc
    13621 tgtccatgtg gttgatgacc tgacatatga ggtgattgct ttatatgagg atcatacatt
    13681 agaagagatc atcaaagaat tatcatcctg ggatgagaaa gatgtcaaag aatcttatgg
    13741 agaagttaca gaattaaaag atgcaggtgt attatttaca gaagactgtt atgaagaata
    13801 catttgtgga ttcaaagaca gaccaacagt tgtaaaggca ctctgtcttc atattgcaca
    13861 tgattgtaac cttgcctgcc gatactgttt cgcagaagaa ggagaataca agggaagacg
    13921 tgcattaatg agtgctgaag tcggtaagaa agcattagat tttcttgtag agaattcagg
    13981 aaaccgtaga aatctggaag ttgatttctt tggtggagag cctttgatga actttgatgt
    14041 agtgaaagag atcgttgcat atggacgttc tttggaagaa acacatgata agaagttccg
    14101 ttttacattg acaacaaatg gtgtattact aaacgatgat atcatggaat ttgccaataa
    14161 agagatggat aacgttgtat taagtgtgga tggaagaaaa gaagttcacg attacatgag
    14221 accattccgt aatggaaaag gaagctatga ttttattatc gacaaattca agaaatttgc
    14281 agacagcaga aaccagcaga aatattatgt aagaggaact tttacacatc ataacttaga
    14341 tttctcaaaa gacgttatgc atttagcaga tgcaggattc gaacagattt ctgtagaacc
    14401 tgttgtagca ccagatacag cagattatgc gatcacagaa gaggatcttc caattttgat
    14461 ggatgaatat gatcttcttg caaaagaaat gatcaaaaga gagaaggaag gaaaaggatt
    14521 taccttcttc cactttatga tcgacttaac aggagggcca tgtgttgcaa aacgtttatc
    14581 cggatgtggt tcaggaacag aatatcttgc agtgactcca tggggagatt tatatccatg
    14641 ccatcagttt gtaggagaag aagacttctt aatgggtaat gtttacgaag gagtcaagaa
    14701 agatgagatc gtcaaagaat tcaaggcatg taatgtatac tccaaagaag aatgtaaaaa
    14761 atgttttgca agattctatt gcagtggtgg atgcagtgca aactcttata actttaacgg
    14821 tacgatcgat ggaacttatg agatcggatg taaattagag cgcaagaggg tagaatgtgc
    14881 attaatggta aaggcagcat tggcattaga aggggagaag gagaatgaag aagcgtaata
    14941 gcagtggcaa gtatattgca gggctcattt tactgatcgc tttgtgtgta tttggagcat
    15001 ttatcacaac caagggaatc acaaagaaga agatcggtcg agcaagcaat atcgaacttg
    15061 gattagacct tgcaggtgga gttagtatta catatgaagt agatggcaag aatgtatctg
    15121 atagtgatat gaaagatact gtatacaaac ttcagaagcg tgtagaaggg tacagtacag
    15181 aagcagaagt ttatcaggaa ggaaaagatc gtatcaatat cgagattcct ggagtaacag
    15241 atgcaaacaa gattttagat gagcttggta aaccaggaac actttctttt gctgtccaga
    15301 aacaggtaaa agtaaagaag aatggaaaaa caacgactca gacaacatta gatactgtat
    15361 tgacaggtca aaatgttaag aaagccaaag ctgtaactaa tcagaatcag acaactggta
    15421 agaaagaata tttagttgca cttgaatttg atgctaaggg aacaaaggca tttgcgaaag
    15481 ctacaaaagc caatattgga aaaccaattt atattatcta tgataatcag gtgatcagtg
    15541 ccccaaatgt acaggaagcg atcacaaaag gagaatgtac gatcgatggt atggaaagtt
    15601 atgaagcagc agagaattta gcttcatcaa ttcgaatcgg ttccttacca gtgaaattaa
    15661 acgaattacg ctctaacgta gtatctgcga aattaggagt gaatgcgatc cagacaacgg
    15721 tcaaagcggg agtcatcgga tttgtactgg tatgcttgat catgatcgca ttttatgcag
    15781 tgcctggtat gattgcgtgt gtggcacttg cgatctatat gctgatgatg ttattagcac
    15841 tgaatggatt taatgcgaca ctgacattgc cgggacttgc aggtatcatc ctcggtatcg
    15901 gtatggcagt tgatgcaaac gttatcattt atgccagaat tcaggaagag atcggggcag
    15961 gaaagagtac aggagcagcg atcaaatctg gattcggaaa ggcagcatca gcgatcatcg
    16021 atggtaacgt tacaacattg atcgcagtat tagttttatg gtttaaagga tcaggaacgg
    16081 tcaaaggatt tgctcagaca cttggaatga gtgtattgat ctctatgttt acagcacttg
    16141 tgatcagccg attccttgta tatgcggcat atcattttgg acttcgcgac aagaaatggt
    16201 atggaaaacc aagaacgatc aaaacaagag attttgtccg tacaggtaag aaatacgttg
    16261 cagttgcagg tgtgatcatt ttgatcggac ttgctgcact tccaatgaat cgaagcaaga
    16321 ttggatcaat cttgaattat gatctggaat tctcaggtgg tacagcatct acgatcacat
    16381 tcaaggatga ccagaaagtc aatgattctt tagaaaaaca ggttgtgaaa gcttacgaga
    16441 aagtcagcaa gtcaacctct gttcagagtc agaaagtaaa agcaaataat cagatggttg
    16501 taaagagtgt agaattgaat ctgagccaga gaaaacagat cgaaagtaca ttaaagaaag
    16561 attataaagt aaaatctgta acaacagaga atatcagttc aacgatcagt aatgaaatgc
    16621 aaagagatgc atttatttcc gttgtgatct cagcgatctg tatgctgatc tacattgcga
    16681 ttcgatttaa agacgtgaag tttggatcca gtgcgatcat cgcattgatc aatgacgtat
    16741 tggtcgtatt tgcagcatat tctgttggaa gattatctgt tggtggaaca tttatcgcat
    16801 gtatgttaac gatcattgga tattctatca actcaacgat cgttatcttt gaccgtatcc
    16861 gtgagaatct gaaacttcag acgatccgga caagagatga tatcaaagca cttgtgaatc
    16921 agagtatttc aagtacattg gttcgtacga tcaatacaag tttgacaaca tttgttatgg
    16981 tgttagcgtt attcatctgc ggagtatcct ccttaagaga atttgcatta gcattgatgg
    17041 tgggtgtgat cggaggagca ttctcttctg tattcttaac tggaccactc tggtatatga
    17101 tgaagacgcg aa
//
LOCUS       DAOMUE010000013        16467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_706_length_16467_cov_2.858945, whole genome shotgun sequence.
ACCESSION   DAOMUE010000013 DAOMUE010000000
VERSION     DAOMUE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 16467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16467
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_706_length_16467_cov_2.858945"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLC71_01445"
     CDS             <1..694
                     /locus_tag="PLC71_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM01341.1"
                     /translation="IGGSTLVLQNQCRFLAEQSGATVISIDYRLAPEAPFPAAFDDCK
                     DVVKWLVHHADHWNIDPNNLSIAGESAGGALAVSCGLSEVGKYLKLVIPIYGALDVCS
                     ASDLDYWDYDLYDVIPEHKKYVITRLNRFRNLNGTLQNLYLNDISDAKNPMASPLYAT
                     DLSNLSNVLMIEAEYDYFRLSNDLFAEHLWNADIPCEVIRYQGMDHGFYDRLGYCEQT
                     KDCILEIAKHIK"
     gene            823..2190
                     /locus_tag="PLC71_01450"
     CDS             823..2190
                     /locus_tag="PLC71_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM01342.1"
                     /translation="MNNNKEFLATEPIGKLLLRLAIPTLAAQMINMLYNIIDRIYIGH
                     IPGSGALALTGVGVCMPLIMIISAFAALVGNGGAPRASILMGKKDLDSAENILGNCFT
                     MQIIISVILTLIMFFGNRTFLMAFGASENTIEYAVSYMNIYSLGTIFVQLTLGMNAFI
                     TAQGFAKTGMYSVLIGAVINIILDPIFIFACHMGVRGAALATIISQACSCIWVLSFLF
                     GTRSTLRIKKQNLTLKAPVILPCLALGLSLFIMQASESIISVCFNSSLLKYGGDVAVG
                     AMTILTSVMQFAMLPLQGLGQGAQPIMSYNYGAKNTDRVKGTFFLLLKASLTYSIILW
                     GLVMLFPQVFAGIFTSNPALVIFTKKALRVYMAVMFMFGIQISCQMAFNSLGKAISSI
                     VVAVMRKFVLLIPLIYIMPHIFTTDQTMAVYMAEPVADFIAVTFTAILFSVQFKKALA
                     AIRLS"
     gene            complement(2265..3287)
                     /locus_tag="PLC71_01455"
     CDS             complement(2265..3287)
                     /locus_tag="PLC71_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM01343.1"
                     /translation="MKVMNVAVVGAGIYGINHVNAYTWNPNTNLVAVCDLNKEITDRI
                     AEQYNVKTYNDVNEMLDNEEIDAVSIATPDAFHMDPALAAIRHGKPILVEKPLATTSE
                     DAKKILAEAEKYNVRVAVDYHKRWDPAAINVRNELQNEESGAPIRGYMCMDDIIDVPT
                     EWFNWADKSSPVHFLGTHCYDQVRWYMGCEVEEVYAVGTKKVLKARGVDTYDSIQATL
                     KMENGCYWTVENSWILPKGFAKNNDGRTQILCENAMFRIDSQNRGVEIYNNSKLRTPN
                     SYFILNNCGRPSGFGIEPINDFIYCLQNDLPFIADGNDGLQAELIAEAVHESLEKGVA
                     VKLKRD"
     gene            complement(3326..4150)
                     /locus_tag="PLC71_01460"
     CDS             complement(3326..4150)
                     /locus_tag="PLC71_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HRM01344.1"
                     /translation="MSNEMNTQEEKKVLTQKDLNKAMFRWYMSAEMPLNFENMQGIAF
                     CGSISHILKKLYTKKEDLSEALKRHLLLYNCNVTAGGLILGTTIAMEEQRAKDPSKMP
                     DMAITGLKTGLMGPVAALGDSFDWGIIGTLMKIAAATLAAAGNPLALVVLLLFVGYCI
                     GELILTTNMTYKKGRESIKTIMGSGLMQDVISGANVLAMFMMGAMTASMVTLKTVLKI
                     STVVVQDKLDSIFPNVFPLIVLFLIYWLIRKKKVGTGKIVIGIIAVSILLSFVGIF"
     gene            complement(4143..4907)
                     /locus_tag="PLC71_01465"
     CDS             complement(4143..4907)
                     /locus_tag="PLC71_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRM01345.1"
                     /translation="MNTVIIAIVMGIMYWWCRGMILSYFGFLFISSPVTVGLVAGLLM
                     GQPMQGLIIGGGIALVFAGIFAPGANLPTDECLAATCVIPIAIGSGMNATTAIALAVP
                     VGLLGSFVTNLRKVINTYFVAKANKYAEEGNADAIWRCATIYPALLAIPLLFLPVFII
                     NMVGQDVVINIMKALPTFVTHGLEVAGGVLPALGFALIMNMIGKNKLIPFVFLGYIVV
                     SVGGVNSLTAGIIAICIAFITVFQRQEIAEEVREHE"
     gene            complement(4926..5402)
                     /locus_tag="PLC71_01470"
     CDS             complement(4926..5402)
                     /locus_tag="PLC71_01470"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM01346.1"
                     /translation="MAEIKLVRVDYRLIHGQVVARWLKVCPVRRIVLVDDVLGSDEFM
                     SDIYKMAVPKGTDVDIVTIDKAKEVLDNTDDTVFLISKDIETCLKLVEKGVELPAINV
                     GAVPNEEGRKLITTGVAISQKELDMLEELNSKGIEITVQPMPEISAISFDAVKRKM"
     gene            complement(5408..5818)
                     /locus_tag="PLC71_01475"
     CDS             complement(5408..5818)
                     /locus_tag="PLC71_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM01347.1"
                     /translation="MMIGLIIATHGNSSVELIKGAEMFAGPLSQCRAWTLNPGDDIQK
                     GEQMLEEYVDELDQEEGVLIMADLFGGTPSNLATKIALRRENVETVTGVNLPMIIQFV
                     TERETQTLDELVESCIETAQDGIKCPTKIMKERR"
     gene            complement(5994..8627)
                     /locus_tag="PLC71_01480"
     CDS             complement(5994..8627)
                     /locus_tag="PLC71_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRM01348.1"
                     /translation="MAKENNKDRILKLLKECKNHQTAESIAVQLNIQRNTASGILNEL
                     VREGIVQKEKTRPVIFSYIQPEDQLPEDPFTTFIGADQSLKDAVEKCKLSAGYPNKGM
                     PILLFGSSGVGKSLLAEYIYQYAKFIGTIPEDAPFVVLNCADYANNKELLSSVLFGYK
                     KGAFTGANKDTKGLIEEADKGYLFLDEVHRLSPEGQEKLFRYMDKGIISRMGDSGQNC
                     ELNVRLIFATTEGRETMLDTFLRRIPVDVVLPDFQERTLEEKYELILFLIHQESKTMN
                     TTFQVSSNVINRLLTFQGKGNIGTLKNIIRLSCAKAYNERSKGQEVIPLNLSHLSSDT
                     LLHGNGESIPYINEMIEVSPDQDAVWKISMTEQADVDFSEKVINDLIQEYLEKDISTI
                     KFRKIIYDEVNRIEDIVIDQEIHPYVQNLYTQAVRNILIYLQDNYGLKYSGVMEMVFV
                     KMLYVLNNQNKHTDDRKYEHLAKQLQRVMYRYYKMGLIFYEMLHQAVDYETNPWFVRM
                     FAMLYFYSIARDEMDYTNAIIVSHGPATASSITSTVNKVFETYIFEAFDMEYDTPKKD
                     VVKRIKRYLKNTNTSKGLLIFVDMGSLLDISEDIKDDVEGDLGIVNNITTEMALEAGE
                     LILKHEDLQNIMDTIIEHHVTKKSFVPSKQKPKAILLCCTTGLGTTDKMKMLLQGCLE
                     GIDIDVVEMTYAELSTEGNRCDVFRKYDIQFIITTSKLMIQGVTTLMLNELIDERGEK
                     VIYSTVGRYCDKDKTQRFIENIVRSFTIKNLIGQLTILNPDKIMGDVEETVSKLEILE
                     DTTYSIDQKKMLYIHMCVMVERLILEKGRLPQEDMTDDLKCRESFIKNLKESFSVIEN
                     KYNVSLNEREILMIYYLTENN"
     gene            complement(8814..9311)
                     /locus_tag="PLC71_01485"
     CDS             complement(8814..9311)
                     /locus_tag="PLC71_01485"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HRM01349.1"
                     /translation="MLHIVLHEPEMPANTGNIGRTCVACGAVLHLIEPLGFRLNEKMI
                     KRAGLDYWDKLDVRTYINFEDFLEKNNDPKIYMATTKAKHVYSAPAYEDDEDVYIMFG
                     KESAGIPEEILLNYEETSIRIPMLPHIRSLNLSNSVAIVAYEVLRHQGFKAFQMEGQL
                     HHYKW"
     gene            complement(9342..10511)
                     /locus_tag="PLC71_01490"
     CDS             complement(9342..10511)
                     /locus_tag="PLC71_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM01350.1"
                     /translation="MRDFLSKRVVSLEPSGIRKFFDIVSEMPDAISLGVGEPDFDTPW
                     RVREEGIYSLERGRTFYTSNAGLKELRYEISEYLERKYELVYDPNHEITVTVGGSEGI
                     DIAMRTILDPGDEVIVVQPCFVSYVACVVMAGGTPVIVSLKEEDKFKLKKEQLEAAVT
                     DKTKAMIISFPNNPTGAIMTKEELEPIAEFAKEHDIVVISDEIYSELTYGRDHVSIAS
                     LPEMKDRSIVINGFSKAFAMTGWRLGYVAAPRYMMKQMVKVHQFCIMAAPTTSQYAAI
                     EAMRSCDDEVEEMRTAYNQRRRFLVHEFQRMGIECFEPEGAFYIFPSIKKFGMTSEEF
                     ATKLLNEEKVAIVPGSAFGACGEGYLRVSYAYSIEELKEALGRLERFINHLPKKQ"
     gene            complement(10511..11005)
                     /locus_tag="PLC71_01495"
     CDS             complement(10511..11005)
                     /locus_tag="PLC71_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRM01351.1"
                     /translation="MNTELRELILKLIEKNSRLAIHDLAVMLDVAETEVANEIADMEK
                     EHIICGYHTLINWDNTSKDQLTAMIEVKVTPQRGQGFDRIAERIYNFPEVKAVYLMSG
                     GYDFMVMLEGKTMKEISMFTSSKLAPLESVVSTVTHFVLKKYKDHGTVLEAKKVDERQ
                     AVTP"
     gene            complement(11035..12033)
                     /locus_tag="PLC71_01500"
     CDS             complement(11035..12033)
                     /locus_tag="PLC71_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HRM01352.1"
                     /translation="MKIGKLSESALQKVVFEQLHTRRDEVLVGPGIGEDCAALKLQEG
                     EVFVTSTDPITGTVKEIGRLAVHVTANDLASAGAETIGLMVTALLPPMIKEAQIKKMM
                     QQINAECEKLNIMVLGGHTEITAAVTQPVLSVTGIGKVKEDELILSGGAKPGQDIVVT
                     KYLGMEGTGIIANEKEDELKEWFSDTFIDDAKAFLEDISVVPEGLIARKYASCMHDIT
                     ESGVYGALWEISKASGVGVEVCIEDIPLRQHTIEFCERYDLNPYQLISSGSMLITTDH
                     GRTLVNELEQAGIKATIIGHTTDSRDKVIYRQGKAANLEAPRQDELYKIYSENNRR"
     gene            complement(12036..13511)
                     /locus_tag="PLC71_01505"
     CDS             complement(12036..13511)
                     /locus_tag="PLC71_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRM01353.1"
                     /translation="MKKKIIIAVAVIVILAAGGTFYLNHKVSSAVKDGKIIKGVSCEG
                     ISIGGMTRSEAKDAIESHMKEIHQEKITLYVDDERSSAKIEDLGAFAEADKTVEEAYA
                     LGRSGSIFTKYSDVKEKKHKLPVYRKYDKAKFEKNVKKATKKIVSEPRNASVKRKDGK
                     FVVIKEKTGYTLNMNETFANFKKAVEAEKHQFELDVVKKKAKYTSKDMAEIKDVLGTY
                     TTEYGGSPYGRKVNVANGASKINGSMVYPGETLSVYKTVSPFTKENGYALAGSYENGQ
                     TVQTYGGGICQVSTTLYNAVIRAELKIVERFPHSMTVHYVPRSADAAIAGTHKDMKFK
                     NTFNTPIYIEGKANGSTITFTVYGKKKDPKRTVEFLSETTQVKESSESTVSDNTLAEG
                     QKVLESYGHTGYSARLWKIVKINGKQVSKKVFNTSTYMSTPTVYRVGTKKAEDKKKDS
                     KETTAQKSETTTAAQKSTTAAQKSTTAKRSAAVKSQGSTGN"
     gene            complement(13633..14340)
                     /locus_tag="PLC71_01510"
     CDS             complement(13633..14340)
                     /locus_tag="PLC71_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRM01354.1"
                     /translation="MKDYVAFDLETTGLSPDSDQIIEIGAVKIRDGKISGKYNCIIHP
                     EIEVSDFIINLTGISRDMLRKGIPLKEGVEEFLEFSGDLPVLGHNVMFDYKFMKMAAA
                     SFKYPFEKTGVDTLKVARKLLTGLENKKLETLCAHYHYVNQAAHRAYDDALATAVVFE
                     QMKKEFPTEEEIFQPQQLQFKVKKERPATSKQKKYLENLMKYHAIGECLDIDQMTQRE
                     ASKKIDHIILNYGVMKK"
     gene            14476..15348
                     /locus_tag="PLC71_01515"
     CDS             14476..15348
                     /locus_tag="PLC71_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM01355.1"
                     /translation="MIKLIVSDLDGTLLNSKQQISDRTLRAIKQIQRKGLRLLINTEQ
                     NYFDAKKLLDAYDISCDIACFGGSCIFDTSGDQLHASYIPTKRIPEMLRIFGSCRTFY
                     EIHSTRGLCVLGSKEGYANYLSSEVVPALREEFPSQILDEESYICERLENAHFYDNGQ
                     LLLSENPQIIKITTQSLDQQKLEQLTNSLHTQVPHFAVSHQSPYRLDITAVNALKGAA
                     VHFYTEKYQISLKDTMVIGDSENDYSMLGLPYIESIAMGNADDIIQEICLHHTADNDH
                     DGVAIVLEQILSRQ"
     gene            15450..16331
                     /locus_tag="PLC71_01520"
     CDS             15450..16331
                     /locus_tag="PLC71_01520"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM01356.1"
                     /translation="MIKLISSDMDGTLLDSYRQITPINVEAIKRAQENGINFIINTGR
                     EYPNARGLVEAAGLKCDLICSNGACAFDKDGNLLFEHILDKETVRTIFDAFNKYGMEP
                     ALFTTEGRISLLPLEEREIYTRDVFIPAIQVNHPDMKYTLDDYRELVDDVIFVDGQEA
                     LLNSDHRILKITSNSPDHESLKKLRVELEKIPGLAVVSTVPTDIEITSINAQKGSSLL
                     DYAKKDGLKPEEIIAIGDSENDYSMLSIPGIHSVAMENACDMIRNICVYQTRANTRDG
                     IAYIINCILTDRENFKL"
BASE COUNT         4408 a         3577 c         2667 g         5815 t
ORIGIN      
        1 tattgggggc agcactttgg tcttacaaaa tcaatgccgt tttcttgctg agcagtctgg
       61 tgcaactgta atctccattg attatcgtct agcacctgaa gctccattcc cagctgcttt
      121 tgatgattgt aaagatgtgg tcaaatggtt agtacatcat gctgatcact ggaatattga
      181 tcctaataac ctttctatcg ctggtgaaag tgctggtggt gctctggctg tttcctgcgg
      241 tttatctgaa gttggaaaat acttaaaact tgtgattcct atttatggag ctttggatgt
      301 atgttccgct tctgatcttg attattggga ttatgatcta tacgacgtga ttcctgaaca
      361 taaaaaatat gttatcactc gtttaaatcg ttttcgaaat ttaaatggta cattacaaaa
      421 cttatatctt aatgatattt cagatgcaaa aaatcctatg gcatctcctt tgtatgccac
      481 agatttatca aacctttcta atgtattaat gattgaagca gaatatgatt attttcgatt
      541 atctaatgat ctgtttgccg aacatctatg gaatgctgat attccttgtg aagttatccg
      601 ctatcaggga atggatcacg gattttatga cagacttgga tattgtgaac agacaaaaga
      661 ctgtattctt gagattgcaa aacatataaa ataatatatt gataccaaac agaaatgaat
      721 tcttggttcg taagaattca tttctgtttt tttgtctaca ttttccaatt tatatgtgtt
      781 acaattgatt cttgtattct acatgaagaa aggaaaataa agatgaataa taataaagaa
      841 ttcttggcaa ccgaacccat cggaaagtta ctgctaagac ttgcgatccc gactttggca
      901 gcgcagatga tcaatatgct ttacaatatt attgaccgta tttatattgg acatattcct
      961 ggaagtggcg ctttagctct gactggagtt ggagtttgta tgccgctgat catgatcatt
     1021 tcggcatttg ccgctcttgt tggaaatggt ggtgcccctc gtgcttcgat cttgatgggt
     1081 aagaaagatc ttgattctgc ggaaaatatt cttggaaatt gttttacgat gcagatcatt
     1141 atctctgtga tcttaacttt gatcatgttt tttggcaacc gtacattttt gatggcattt
     1201 ggagccagtg agaatacgat cgaatatgca gtcagttata tgaatattta ttctcttggc
     1261 acgatttttg tacagttaac acttggaatg aatgcattta tcactgcgca aggatttgcg
     1321 aaaactggaa tgtattctgt tttgatcgga gccgtgatca atatcatctt agatccgatt
     1381 tttatctttg catgccatat gggcgttcgt ggtgccgcac tggctacaat catatctcag
     1441 gcttgttctt gcatctgggt attgagtttc ttatttggga caagatcgac acttcgtatc
     1501 aagaaacaaa acctgacact gaaagctcct gtgatcctgc cttgtctggc tcttggactt
     1561 tctttgttta tcatgcaggc aagtgaaagt atcatctctg tgtgttttaa ctcttctttg
     1621 cttaaatacg gtggtgatgt ggctgttggt gcaatgacga tccttactag tgtaatgcag
     1681 tttgcgatgc ttcctcttca gggacttgga cagggtgcac agccgatcat gagttataat
     1741 tacggtgcaa agaatacgga tcgtgtaaag ggtactttct tcctcttatt aaaggcaagt
     1801 ttgacttact ctatcatctt atggggactc gtcatgttat tccctcaagt gtttgctggt
     1861 atttttactt caaatccagc tcttgttata tttacaaaga aggctcttcg tgtttacatg
     1921 gcagttatgt ttatgtttgg aattcagatt tcctgccaga tggcatttaa ctctcttgga
     1981 aaagcaattt cttctattgt tgttgctgtt atgagaaaat ttgtactttt aattcctttg
     2041 atctatatca tgcctcatat ttttacaaca gatcagacca tggctgttta tatggcggaa
     2101 cctgttgctg attttattgc agtaacattt acagcgatct tgttttctgt acaatttaag
     2161 aaagctcttg ctgcgatccg tttatcctaa aaaagaaaag tctggaaaat tctaatactt
     2221 ttccagactt ttctttttat catagggcca tagacgaaag agtcttaatc tctctttaat
     2281 ttcactgcca cacctttttc aagactttca tgaactgctt ctgcgatcaa ttctgcctgt
     2341 aatccatcgt ttccatcagc tataaatgga agatcattct gtaaacagta gataaaatcg
     2401 ttaattggtt caattccaaa tccacttggt cgtccacagt tatttaagat gaaataagag
     2461 tttggtgtgc gtaatttgct attgttatag atttctactc cacgattctg agagtcgatc
     2521 ctaaacattg cattttcaca caggatctgt gttcttccat cattattttt tgcgaatcct
     2581 tttggtaaga tccaagaatt ttctactgtc cagtagcatc cattttccat tttcagtgtt
     2641 gcctggatag agtcgtatgt atcaactcct cttgctttta acactttctt tgttcctact
     2701 gcgtatactt cttctacttc gcatcccata taccaacgaa cctgatcata acaatgtgtt
     2761 cctaagaaat gtacagggct tgatttgtct gcccagttga accattctgt tggaacatca
     2821 atgatatcat ccatgcacat atatccacgg attggtgctc ctgattcttc gttctgaagt
     2881 tcatttcgta cattgatcgc cgctggatcc catctcttat gatagtcgac cgcaactctt
     2941 acattgtatt tttcagcttc tgctaaaatt ttctttgcat cttctgatgt tgttgctaat
     3001 ggcttctcca ccagaattgg ttttccatga cggatcgctg ctaatgccgg gtccatgtgg
     3061 aaagcatctg gtgttgcaat agatacagca tcaatttctt cgttgtctaa catttcattt
     3121 acgtcattgt atgtttttac attgtattgt tctgcaatgc gatctgtgat ctctttattt
     3181 aagtcacaca ctgcaactaa atttgtgttt ggattccacg tatatgcatt tacatggtta
     3241 attccataga ttcctgctcc tactactgca acattcataa ctttcatttt aaatctcctt
     3301 tttaactaca tgacacttta attttctaga aaattcctac aaaagaaagc aaaatggaaa
     3361 ctgcgatgat tccaattacg atctttccgg ttcctacttt tttctttctg atcaaccagt
     3421 agatcaagaa taaaacgatc agtgggaata catttgggaa aatagaatct aacttatcct
     3481 gtactacgac tgtactgatc tttaacacag tttttaatgt taccatggaa gcggtcatag
     3541 cacccatcat aaacatggcg agtacattgg ctccggagat aacatcctgc attaaaccag
     3601 atcccatgat cgttttaata gattctcttc cctttttgta tgtcatattt gttgttaaga
     3661 tcaattcacc aatacaatat ccaacgaaca gtaataatac gactaatgcc agtggatttc
     3721 ctgctgccgc aagtgttgct gctgcgatct tcatcaatgt tccgatgatt ccccagtcaa
     3781 agctgtctcc taaagctgcg acaggtccca tcaatcctgt ttttaaacct gtgattgcca
     3841 tatcaggcat cttagatgga tctttcgctc tctgctcttc cattgcgatg gttgttccaa
     3901 ggatcagacc acctgctgtt acattacagt tatataataa taggtgacgt tttaaggctt
     3961 ctgataaatc ttcttttttg gtatataatt tctttaaaat atgtgaaata cttccgcaaa
     4021 atgcaattcc ctgcatattt tcaaagttca gaggcatctc agcggacatg taccaacgga
     4081 acattgcttt atttaagtct ttttgtgtta agactttctt ttcttcttgt gtattcattt
     4141 cattactcat gttctcttac ctcctctgca atttcctgtc tctggaatac tgtaataaat
     4201 gcaatacaga ttgcgataat tccagctgtt aatgagttta ctcctccaac ggatactacg
     4261 atataaccta agaatacgaa aggaatcagt ttgttctttc cgatcatatt catgatcagt
     4321 gcgaatccta aggctggtaa aactcctcct gctacttcta atccatgtgt cacaaatgtt
     4381 ggaagtgcct tcataatgtt aattactaca tcctgaccta ccatgttaat aataaacact
     4441 ggtaagaata gtagtggaat tgctaataac gctggataaa tcgttgcgca tctccagata
     4501 gcatctgcat ttccttcttc tgcatattta tttgcttttg ctacaaagta tgtgttgatc
     4561 acttttctta aattggttac gaatgatccg agtaatccaa ctggtactgc taatgcgatc
     4621 gctgttgttg cattcattcc tgatccgatt gcgatcggaa taacgcatgt tgctgccaga
     4681 cactcgtctg ttggaaggtt cgctcctggt gcaaaaatac ctgcaaatac cagtgcgata
     4741 ccaccaccga taattaatcc ttgcattggc tgtcccatca gtaaccctgc tacaagtcct
     4801 accgttacag ggcttgagat aaacaggaat ccgaaatatg ataagatcat tcctcggcac
     4861 caccaataca taattcccat gacaattgct atgattacag tattcatata tttttcctcc
     4921 ttttgttaca tcttacgttt tactgcatcg aaacttattg ctgatatttc aggcattggc
     4981 tggacagtga tctcaatacc tttactattt aattcttcca gcatatcaag ttccttttgt
     5041 gaaattgcaa ctcctgttgt gatcagtttt cttccttcct catttggtac tgctccaaca
     5101 ttgatcgcag gtaattcaac tcctttctct actaatttca gacatgtctc aatatctttg
     5161 gaaatcaaaa ataccgtatc gtctgtgtta tctagcacct cttttgcttt atcaatggtt
     5221 acaatatcaa catctgttcc ttttggaact gccatcttgt aaatgtcact cataaattcg
     5281 tcacttccaa gtacatcatc tactaatacg attcgtctta ctgggcaaac ctttaaccat
     5341 cttgccacta cctgtccatg gatcagtctg taatctactc tcactaattt aatttctgcc
     5401 ataattctta cctccgctct ttcattattt ttgttggaca tttaattcca tcctgtgctg
     5461 tttcgataca actttctacc agttcatcta atgtctgcgt ttctcgctct gtcacaaact
     5521 gaatgatcat tggaagattc actcctgtta ctgtttcaac gttttctctc ctcaaggcaa
     5581 tttttgttgc caggttggaa ggtgttcctc caaacagatc tgccatgatc aagactcctt
     5641 cttcctgatc taattcgtct acgtattctt ctaacatctg ctcccctttt tgaatatcat
     5701 ccccagggtt tagggtccat gctctgcact gacttaatgg tcctgcaaac atctctgctc
     5761 ctttgatcaa ttcaacactt gaattgccgt gtgttgcaat gattaatccg atcatcatac
     5821 ctcctctctt ggtcatccat atttctattt cgacacattt taattttttg tttttcacat
     5881 cttacacttt tttataaagc aagatccgtg ccaacttttt atttttttgc aaaataaaaa
     5941 aagcacatat tcattttttc gcataaatac gtgctttttt catcttaaaa tttttaatta
     6001 ttttctgtca gataatagat catcaagatc tccctctcat tcagcgacac attgtacttg
     6061 ttttctatga cactaaaact ctcttttaaa ttttttatga agctttcccg acacttcaga
     6121 tcatctgtca tatcttcctg tggcagcctt cctttttcaa gtatcaatcg ttctaccatg
     6181 acacacatat gaatatacag catctttttt tgatcgatcg aataagttgt atcttctaaa
     6241 atctccagct tgctgacagt ttcttcgaca tctcccatga tcttatctgg attcaggatc
     6301 gtaagctgcc cgatcagatt ctttattgta aagctcctta cgatattctc aataaatcgt
     6361 tgtgtcttgt ccttgtcaca atatcgtcct actgtggaat aaatgacttt ctctcccctc
     6421 tcgtcaatca attcatttaa cataagtgtc gtaacgcctt ggatcataag cttgcttgtc
     6481 gttatgataa actgtatatc atatttacga aagacatcgc atcggtttcc ctccgtactt
     6541 aattctgcat aagtcatctc cacaacatca atatcaatgc cttccaggca tccttgcagc
     6601 aacatcttca tcttatctgt cgttcctaaa cctgtcgtac agcataaaag aattgctttt
     6661 ggtttttgtt tgcttggaac aaatgatttc tttgtaacat gatgctcgat gatcgtatcc
     6721 ataatatttt gaagatcttc atgttttaag atcaattctc ctgcttccaa tgccatttct
     6781 gtcgtaatat tattgacaat ccccaaatct ccttcgacat catcttttat gtcctctgaa
     6841 atatcaagta atgatcccat atcaacaaag atcaacaacc ctttagaggt attcgtattt
     6901 ttgagatatc gcttaatacg ttttactaca tctttctttg gcgtatcata ttccatatca
     6961 aaagcttcaa atatataagt ttcaaatacc ttattgacag tacttgtaat actagatgcc
     7021 gtcgctggtc catgtgatac gatgatcgca tttgtataat ccatctcatc ccttgcaata
     7081 ctgtaaaaat ataacatcgc aaacatcctt acaaaccatg gatttgtctc ataatctact
     7141 gcctgatgaa gcatctcata gaaaataagt cccatcttat aataacggta catcactctc
     7201 tgcaattgtt ttgccagatg ttcgtatttc ctgtcgtctg tatgcttatt ctgattattc
     7261 agtacataaa gcatctttac aaataccatt tccatcacac cactgtattt gagaccgtaa
     7321 ttatcttgaa gatagatcaa aatatttctc actgcctgtg tatacagatt ctgcacatat
     7381 ggatgaattt cctgatcgat cacgatatct tcgattcgat tcacttcatc ataaatgatc
     7441 tttcgaaact taatggttga tatatctttc tcaagatatt cttggatcag atcattgatc
     7501 actttctccg aaaaatccac atccgcctgt tctgtcatag aaatcttcca cactgcgtcc
     7561 tgatctggtg acacttcgat catttcattg atatatggaa ttgattctcc atttccatga
     7621 agtaatgtat cggaagacag gtggcttaaa ttaagaggta tcacttcctg tcctttactt
     7681 ctttcattgt atgcctttgc acaggataat cgaatgatat tttttaacgt tccaatattg
     7741 ccttttcctt ggaatgtcag caatcgattg atcacattac tgctcacctg aaatgtagta
     7801 ttcatcgttt tggattcctg atggatcaaa aacaagatca attcatactt ctcttccaaa
     7861 gttctttcct gaaaatctgg cagcactaca tctactggaa ttcttcttag aaatgtatcc
     7921 agcatagttt ctctgccttc tgttgttgca aagatcagtc gaacattcaa ttcacaattt
     7981 tgtccactgt ctcccattct ggaaatgatc cctttatcca tatatcgaaa taacttttcc
     8041 tgtccttctg gcgataggcg atgaacttca tccaaaaaca gatacccttt gtctgcttct
     8101 tcaatcaggc cttttgtatc cttattggcc cctgtaaacg ctcctttttt gtatccaaat
     8161 aagacagacg ataataattc tttattattc gcataatccg cacagtttaa tacaacaaaa
     8221 ggggcatcct ctggaattgt tccaatgaat tttgcatatt ggtagatgta ctctgccaaa
     8281 agactttttc caactccact tgatccaaat aataaaatcg gcataccctt gtttggataa
     8341 cctgccgata acttacactt ctctactgcg tcttttaaac tttgatctgc tccaataaat
     8401 gttgtaaatg gatcctctgg taattgatct tctggttgta tgtaagaaaa tataaccggc
     8461 cgtgtttttt ctttctggac aattccttct cttacaagct catttaaaat tccactcgct
     8521 gtgtttctct gaatatttaa ttgtactgca atactctccg ccgtttgatg attcttacat
     8581 tccttcaaca atttcagaat acgatcctta ttattttctt ttgccatact ttttcttatc
     8641 ctttcttatt tcgtactcca agtctcgtct tctttgttgt tcttaaatat atacaattct
     8701 tattctcatc ataccacaca tgatcggcaa tttgttaaaa tatttgccac ttgaatatta
     8761 ttatgttaaa aaaggaactg catgatccat ttgcagttcc ttctattatc attctaccat
     8821 ttatagtgat gcagctgtcc ttccatctga aatgccttaa atccctgatg tctcagcact
     8881 tcataggcaa cgatcgctac agagttagat aaatttaatg accggatatg tggcagcatt
     8941 gggattctta tagaagtctc ttcataattc aataagattt cttctggaat tccagcgctt
     9001 tcttttccaa acatgatata tacatcttcg tcatcttcat atgctggtgc cgaatataca
     9061 tgttttgctt ttgttgttgc catgtaaatc ttgggatcat tgttcttctc tagaaaatcc
     9121 tcaaaattga tatatgtacg tacatctaat ttgtcccaat aatcaagacc ggctctcttg
     9181 atcatctttt cgtttaaacg aaatcctagt ggttcgatca gatgcagaac agcaccgcat
     9241 gccacgcagg ttcttccgat atttccagtg tttgccggca tctccggctc atgtaatact
     9301 atgtgaagca ttattaactc tccaatctct taacttttta tctattgctt ctttggaaga
     9361 tgattgataa atcgttccaa acgccctaaa gcttctttta gttcttcaat ggaatatgca
     9421 taggaaactc tgagatatcc ttctccacaa gccccgaatg cagatcccgg aacgattgct
     9481 actttctctt catttaacag ttttgttgca aattcttcag atgtcatgcc aaatttctta
     9541 atagatggaa agatgtaaaa ggcaccttct ggttcaaaac attcgattcc cattcgttga
     9601 aactcatgta ccaaaaatcg tctgcgctga ttgtatgcag tcctcatctc ttcgacttca
     9661 tcatcacagg aacgcatcgc ttcgatcgct gcatactgac tagtcgtcgg cgctgccatg
     9721 atacagaact gatgaacctt gaccatctgt ttcatcatgt accttggtgc tgcaacataa
     9781 ccaagtctcc atccagtcat tgcaaatgcc ttggaaaatc cattgatcac gatcgatctg
     9841 tctttcatct ctggcaagga tgcgatactg acgtgatctc ttccataggt aagttctgaa
     9901 taaatctcat cagaaattac aacaatatca tgttcttttg caaactctgc gattggttct
     9961 aactcttctt ttgtcatgat cgcacccgtt ggattgtttg gaaatgaaat gatcattgct
    10021 tttgttttat ctgtgacagc agcttctaac tgctcttttt taagcttaaa tttatcttct
    10081 tccttaagac ttacgatcac aggagttcca cctgccataa caacacatgc cacataagaa
    10141 acgaaacatg gctgtacaac gatgacttca tctcctggat caaggattgt acgcatcgca
    10201 atatcaatcc cttcactccc gccaacggtc actgtgatct catgatttgg atcatagacc
    10261 aattcgtatt ttctttctaa atattcactg atctcatagc gcagctcctt taaccctgca
    10321 ttcgatgtat aaaaggttct gcctcgctcc aatgagtaaa ttccttcttc cctgactctc
    10381 catggtgtat caaaatccgg ttcccccaca cctaaggaaa tggcatctgg catctcgctt
    10441 acaatgtcaa aaaacttgcg gattcctgat ggttccagac ttacaactct ctttgataaa
    10501 aaatctctca ttatggagtc accgcctgtc tctcatccac tttctttgct tcaagaacag
    10561 ttccatgatc tttatatttc ttcaatacaa aatgtgttac tgtactgaca acagattcca
    10621 gtggtgctaa tttggatgat gtgaacatag agatctcttt catcgtcttt ccttctaaca
    10681 taaccatgaa atcatatccc ccagacatca gataaacggc ttttacttct gggaaattat
    10741 aaatacgttc tgcaatgcgg tcgaatccct gacctctttg tggtgttact tttacctcga
    10801 tcatcgctgt cagttgatcc ttgcttgtat tgtcccagtt gatcaaagta tgatacccac
    10861 agatgatatg ttctttttcc atatctgcga tctcattggc gacttccgtc tccgcgacat
    10921 ctagcatcac cgccagatca tggatcgcta aacgactgtt tttctcgatc aattttaaaa
    10981 tcagttctct taattctgtg ttcatactgt ttcctccatt tttctctctg aaaattatct
    11041 gcgattattt tcagaatata ttttatataa ctcgtcctgt ctaggtgcct ccagattcgc
    11101 tgcctttccc tgacgataga ttactttatc tctggaatct gttgtatgac caataatggt
    11161 tgctttgatg cctgcctgtt ccagttcatt gactaatgtt cttccatgat ctgttgtgat
    11221 cagcatagat ccacttgaga tcaattgata tgggttcaga tcatatcgtt cacaaaattc
    11281 gatcgtatgt tgtcttaatg gaatatcttc aatgcaaacc tccaccccga caccagatgc
    11341 tttggaaatc tcccataaag caccataaac tccgctttct gtgatatcat gcatacaaga
    11401 cgcatatttt ctggcgatca gaccttctgg cacgacagag atatcctcta gaaatgcctt
    11461 tgcatcatca ataaatgtat cagaaaacca ttcttttaac tcgtcttctt tttcatttgc
    11521 gatgattccc gttccttcca taccaagata ttttgtgact acgatatcct gacctggttt
    11581 cgccccgcca gataagatca attcatcttc tttcactttt ccgattcctg tcacagataa
    11641 tactggctgc gtaaccgctg ccgtaatctc tgtatggccg cctaatacca tgatatttaa
    11701 tttctcacac tctgcattga tctgctgcat catcttcttg atctgtgctt ccttgatcat
    11761 tggtggcaga agtgctgtca ccataagtcc gatcgtctca gctcctgcgg atgcaagatc
    11821 gttggcagtt acatgaactg ccagacgtcc gatctctttt accgtgcctg tgatcggatc
    11881 tgttgatgtg acaaatactt ctccttcctg aagtttcaat gccgcacaat cttccccgat
    11941 tccaggtcct actaagactt catctctcct tgtatgcaat tgctcaaaca caactttctg
    12001 caatgcagat tctgataatt ttcctatttt catccttaat ttcctgtgct tccctgagat
    12061 ttcacagccg ctgatctctt cgctgttgta gacttctgtg ctgccgttgt agatttctgt
    12121 gctgctgttg ttgtctcaga cttctgtgct gttgtttctt ttgaatcttt tttcttatct
    12181 tccgctttct ttgttcctac acgataaacc gttggtgttg acatatatgt ggaagtatta
    12241 aatacctttt tggatacctg tttaccattg atcttcacga tcttccaaag tcttgcactg
    12301 tatcctgtat gtccatagct ttccaaaact ttctgtcctt cggcaagcgt attatcgcta
    12361 actgtacttt cagaactttc ttttacctgt gttgtctcag ataagaattc taccgtacgt
    12421 tttggatctt ttttctttcc atagaccgta aatgtgatcg ttgaaccatt tgcttttcct
    12481 tcaatataga ttggagtatt aaatgtattc ttaaacttca tatctttatg agttcctgcg
    12541 atcgctgcat ctgcagatct tggtacataa tgtacggtca tggaatgtgg aaaacgttct
    12601 acgatcttta attctgcacg gataactgca ttatacaaag tagtagatac ctggcagatt
    12661 ccaccaccat atgtctgaac cgtctgtcca ttttcatagg aacctgcgag tgcatatcca
    12721 ttttcttttg taaatggtga tacggtttta tatacagata atgtctctcc tggataaacc
    12781 atgcttccat tgatcttact tgcaccatta gcaacattta ctttacgacc atatggagat
    12841 ccaccgtatt cggtcgtata agttccaagt acatctttga tctctgccat atcttttgat
    12901 gtatattttg cttttttctt tacaacatcc agttcaaatt gatgtttctc agcttcaact
    12961 gcttttttga aatttgcaaa tgtttcattc atatttaatg tataacctgt cttttctttg
    13021 atcacgacaa atttgccatc cttacgtttc acacttgcat ttcttggttc tgaaacaatc
    13081 ttctttgttg ctttttttac attcttctca aacttcgctt tatcatattt gcgataaacc
    13141 ggaagtttat gtttcttctc ttttacatcg gaatactttg taaaaatact tccgcttctc
    13201 ccaagtgcat aagcttcttc cactgtcttg tctgcctcag cgaatgctcc aagatcttcg
    13261 atcttggcac ttgatctttc atcgtctaca taaagtgtga tcttttcctg atgaatctcc
    13321 ttcatatgag attcaatcgc atcttttgct tctgatcttg tcattccacc gatagaaatt
    13381 ccctcacatg aaaccccttt tatgatcttt ccatctttta ctgcactgct caccttatgg
    13441 tttaggtaaa aagttccacc tgctgccaaa ataacgatca ctgcaaccgc aatgataatt
    13501 tttttcttca tcgtctcttc cttcttcctc tccaaattca ttcttacatc atcaaatatc
    13561 ctgctaccat tgttccaacc atcacaatga tcagaagaat cgcaataacg gatacaaaaa
    13621 atcttttctt tttcatttct tcatcactcc ataatttaat ataatatgat caatcttctt
    13681 tgatgcttct ctctgtgtca tctgatcaat atctaagcac tcccctatcg catgatattt
    13741 catcagattc tctagatact tcttttgttt tgatgtagcc ggacgttctt tctttacctt
    13801 gaattgaagc tgctgtggct gaaagatttc ctcttctgtt ggaaattctt tcttcatctg
    13861 ctcaaacaca acagctgttg ctaatgcatc atcgtaagca cggtgtgctg cctgattcac
    13921 ataatgataa tgagcacaga gtgtctccag cttcttattt tcaagtccag ttaataactt
    13981 ccttgctact tttagcgtgt caactcctgt tttctcaaac ggatacttaa atgaagctgc
    14041 tgccatcttc ataaacttat agtcaaacat gacgttatgt ccaagaaccg gaagatcccc
    14101 gctaaattcc aaaaattcct caacaccttc tttcagtgga attcccttcc ttaacatatc
    14161 cctgctgatt cctgttaaat tgatgataaa atcactgact tcgatctctg gatgaatgat
    14221 acagttatac tttccactga tcttcccatc tctgatctta acagctccaa tttcaataat
    14281 ctgatctgaa tctggggata accccgttgt ctccagatca aatgcaacat aatctttcat
    14341 ctgtcgttac ctctgagaat ttatacttat ttgtgcaagc acaaacgcaa tttatactta
    14401 ttgacacttt atttttatgc ttttttatgt tacgattaac ctagatataa ttagaaaaca
    14461 aaggaggacg gcaagatgat aaagctgatc gtttctgatc ttgacggaac actgcttaac
    14521 agcaaacaac aaatcagtga tcgtacactc cgtgcgatca aacaaattca aagaaaggga
    14581 ctccgtcttt tgatcaatac ggaacagaat tattttgatg ccaaaaaact cctcgatgca
    14641 tacgatattt cttgtgatat cgcatgtttt ggcggttcct gcatctttga tacttctggg
    14701 gaccagctgc atgcttctta tattccaacc aaacgtatcc ctgagatgct gcggatcttt
    14761 ggttcctgtc gtacttttta tgagatccac agtacccgtg gattatgtgt cttaggttcc
    14821 aaggaaggat atgcaaatta tctgtcttct gaagttgttc ccgctttgag agaagaattt
    14881 ccaagccaaa tactagacga agagtcttat atctgtgaac gacttgaaaa tgctcatttt
    14941 tatgataatg gacaacttct tctttctgag aatccacaga tcattaaaat cacgacacag
    15001 tccttagacc agcagaaact tgaacagctt acaaacagcc ttcatacgca ggtcccacac
    15061 tttgcagtat cccatcagag tccatatcgt ctcgatatca cagctgtaaa tgcattaaaa
    15121 ggtgcagccg ttcattttta tactgagaaa tatcagattt cactgaaaga taccatggtg
    15181 atcggtgaca gtgaaaatga ttattctatg cttggacttc cttatatcga aagcattgct
    15241 atgggaaatg cagatgatat cattcaggag atctgtctgc atcatacagc tgataacgat
    15301 catgacggcg ttgcgatcgt gctggaacag attctttcca gacaatgatc attgaaatat
    15361 attttaaagc aatttggaat gttttatcta ttccaattgt gatactataa agatgagaag
    15421 aaattacacc caatacagga ggctatcaca tgattaaatt aatttcatcc gatatggatg
    15481 gtactcttct tgacagctat cgtcagatta ccccgatcaa tgtcgaagcg atcaaaagag
    15541 ctcaggaaaa tggtatcaat tttattatca acacaggacg tgaatatcca aatgcacgcg
    15601 gtcttgtgga agcagccgga ttaaaatgcg acttaatctg cagtaatgga gcttgtgctt
    15661 ttgataaaga tggaaatctt ttattcgagc atattcttga caaagaaact gtccgcacca
    15721 tttttgatgc atttaataaa tacggaatgg aacctgcatt atttacaaca gaaggacgta
    15781 tttctcttct tccattagaa gaacgtgaaa tttatacaag agatgtattc attcctgcca
    15841 ttcaggtcaa tcacccagat atgaaatata ctttagatga ttaccgtgag ttagttgatg
    15901 atgttatctt tgttgatggt caggaggcgt tattaaacag tgaccaccgc atcctaaaga
    15961 tcacatcaaa ctctccagat catgaatctt taaagaaatt acgtgtagaa ctcgaaaaga
    16021 ttccgggact tgctgttgta tctacggttc caacggatat tgagattact tctatcaatg
    16081 ctcagaaggg aagctcttta ctagattatg caaaaaaaga tggattaaaa ccagaagaga
    16141 tcatcgcgat cggtgacagt gaaaatgatt attctatgtt atctatccct ggtattcatt
    16201 ccgttgctat ggagaatgct tgtgatatga tccgcaatat ctgtgtttac cagacacgtg
    16261 cgaatactcg tgatggaatt gcttatatta ttaattgtat acttacagat cgagagaatt
    16321 tcaagttgtg atctgtaagt agtgttggtc atgtatgagt atttattata atttcctgtc
    16381 tactgcaaca taacctgaaa accaataacg tccaccagct tgaattttct taattttata
    16441 attgaaagtc ttcaggtatt tagagaa
//
LOCUS       DAOMUE010000014        15602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_773_length_15602_cov_2.848974, whole genome shotgun sequence.
ACCESSION   DAOMUE010000014 DAOMUE010000000
VERSION     DAOMUE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 15602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15602
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_773_length_15602_cov_2.848974"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..234
                     /locus_tag="PLC71_01525"
     CDS             61..234
                     /locus_tag="PLC71_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HRM01357.1"
                     /translation="MIPKDPVMLLSYINTQLRDFYDSYEDLCKSLDLDQNEVKQKLSM
                     IGYEYDESLNQFK"
     gene            292..993
                     /locus_tag="PLC71_01530"
     CDS             292..993
                     /locus_tag="PLC71_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM01358.1"
                     /translation="MELIRLENVKKIYQTGEVITKAVDGIDFTVDQGEFALIIGASGA
                     GKTTVLNILGGMDTATSGAVYIDGEDISKWNKKQLTGYRRDDIGFVFQFYNLVPNLTA
                     TENVELALQISKNPLKAEEVLKDVGLGHRLNNFPAQLSGGEQQRVSIARALAKNPKIL
                     LCDEPTGALDYQTGKSILKLLQDTCHKKGKTVVVITHNLAIAPMADRVIEMKNGKISN
                     ITINEHPQPVETIEW"
     gene            1003..>3969
                     /locus_tag="PLC71_01535"
     CDS             1003..>3969
                     /locus_tag="PLC71_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRM01359.1"
                     /translation="MKKTALHKDIYRTITGTLPRFLSIFFIVVLGVAFYSGIRVTQKD
                     MHITADHQFDGSRLMDVKVMGTLGISEENLTALRKLKNVQSVEGGHSVDVIARKQGSD
                     EEHAIKVMGKTDKLNQITIIDGKLPQASGECLVDEWYAQQNDLKTGDVLNLSSGNEDD
                     LKDTLKDTTYKITGIGSSSEYLSRSRGSTGIGTGTLSGFIVVQPSEFSSDIYTEVYLT
                     AKGAKQERAYSDAYKNKVKQLEEEIKDISKIENEKRLRSVQKEAEEKIDKKEKKFKKE
                     RKKAYKKLDQAEKKLKKSRQKIKDSKAKLAKSKKEISDGEQQIKNAKAQYQSGIRQIQ
                     NARKQLKTQKQTIRSKSKELKTQEAKLKTQEKNLQTKEQQIQGAMQAGMISQDEAKAS
                     LAQINAGKEQIKTARSKINAGKKQISRAKTKIAKAETTLDQKSSQLKKAKQTIKTNEA
                     KLVSARKQITSGEKKIAKAESKLSSGEKKLKRSRKKAEKKFKEAEQKIADARQDVKDI
                     KKGKWYVLNREKMQSYVEYGQEADRIAALGRVVPVIFFLVAALVSLTAMTRMVEEQRT
                     QIGMMKALGYSGVHIAMKYVTYALAATLSGSILGAVIGEKLLPWIIINAYKMMYTGLG
                     DVYTPLEIEYSVMAGGLAVGVVVLAVLSACYKELREKPAQLMRPVAPKEGKRILLERI
                     PFIWKRLSFIWKATMRNLFRYKKRFFMTIFGIGGCMALLLLGFGIKDSISAISEKQYG
                     EIITYDFSITHKDGISETKKEDLIQYAKKQEHMTDLIEVSESAVTIRANGKKQDATMI
                     QPMSKKDLNGYISLQDRKSKTKYQLDDDGIILTEKAASLLGVKKGDSIKIQRSGDKQI
                     TAKISQITENYMQHKVYMTPKLYEKLYHKKAQTTGIYCIEKNISKKKMQENGKQILAR
                     DEASTLHFCADDEKTMSDMVNNLNIVVVVLIVSAGLLAFVVLYNLNNINISERRMELA
                     TIKVLGFYDGEVLQIRS"
     assembly_gap    3970..3982
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3983..4248
                     /locus_tag="PLC71_01540"
     CDS             <3983..4248
                     /locus_tag="PLC71_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM01360.1"
                     /translation="EVGAYVYRENILLTILGTIAGIILGIILHKYVIFTTEVDLIMFG
                     RQIYVQSYVYSILLTIAFSILVNFFMYFQLKKIDMVESLKSTE"
     gene            4267..5583
                     /locus_tag="PLC71_01545"
     CDS             4267..5583
                     /locus_tag="PLC71_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM01361.1"
                     /translation="MKMNELMIYQVFPLRALTDGQPSHGILEMIDWIQHIKKLGMNAV
                     YFSPVFSSSDHGYDTKDYQKIDERLGTNEDFKKMCDAMHEEGIQVILDGVFNHVGREF
                     FAFEDVREKKWDSPYKDWFYINFDGNSCYDDGFWYEGWEGHFELVKLNLDNEQVRNYL
                     LESVGIWIDKLGIDGLRLDVAYMLNRNFMKVLVDFVHSKKPEFLMIGEMLHGDYTQLV
                     NPELLDSVTNYECYKGLYSSFNTHNMHEIGYSLNRQFGPEEWTLYKGLPLYNFVDNHD
                     VERIASQLEDKEHLPLIYAMEFAMPGVPGVYYGSEWGMEGKKEQGSDDSLRPRIHKED
                     LVENELTNYIAQLAKVRAGSPALKYGDYLQLAIAPDVLVFQRRTNEDRIIFAMNCGDG
                     EFTAHFDAQAGCGIDLITGDSVDFGGGLTIQGKTAMIIRTEEVSLP"
     gene            5653..7200
                     /gene="rny"
                     /locus_tag="PLC71_01550"
     CDS             5653..7200
                     /gene="rny"
                     /locus_tag="PLC71_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRM01362.1"
                     /translation="MVGIIIAIIATLVIAVPVSAYVANTRSKKAMEITIGNAEDKARE
                     IIDEAIKAADAKKREASLEVKEETIKARHDFEKETKERRAELQKYEQRVLSKEETVEK
                     KATVLERKEQSLTAKEKNIDTEKAQLQELQAKHLQELERISGLTSDQAKEQLLSAVKE
                     DVKHETAMYVKEMETRAKEDARKKAKEYVVTAIQKCAVDHVAETTISLVQLPNDEMKG
                     RIIGREGRNIRSIENATGVELIIDDTPEAVVLSSFNPVRREIARIALEKLIVDGRIHP
                     ARIEEMVEKATKEVETMMREEGEAATLELGVHGIHPELVRLLGRMKFRTSYGQNALKH
                     SMEVAQLSGLLAAEIGVDVRMAKRAGLLHDIGKSIDHEVEGSHVELGVNLCKKYKENP
                     IVINAVASHHGDEEPESLIACLVQAADAISAARPGARSETLESYTTRLKQLEEIADSF
                     QGVDKTFAIQAGREIRVMVVPEQISDDDMILLARDISNQIEENLDYPGQIKVNVIRES
                     RVVDYAK"
     gene            7279..7809
                     /locus_tag="PLC71_01555"
     CDS             7279..7809
                     /locus_tag="PLC71_01555"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRM01363.1"
                     /translation="MKGYEKLEQHLIHQGKVVGFYEDIVKLPSGKVVTWDLVKHKGAA
                     AVVPVTDKGTILLVRQYRNALDQETLEIPAGGIEPGETPLECVTREIEEETGFIAGKM
                     THLMTVITAIGFCDEKIPIYVATDLKLSKQHLDEDEFIDVEEYTIDEIKDMIFSGKII
                     DAKTISGVLGYLNMEK"
     assembly_gap    7931..7940
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7941..8451
                     /locus_tag="PLC71_01560"
     CDS             <7941..8451
                     /locus_tag="PLC71_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01364.1"
                     /translation="GSVFTNFVYQDYLAYGGTVRSLVLNNTDLYKASISLFFYILFKR
                     GKQYAFIYLGAYLLQPWIFLYSFAFCMAFFFGSMLSLQIVQMGIKGLVLVFMSLFPHF
                     ICYVITLLLLIKRNFYAQKKEEMLYEQRHSFLFRFSIWFEITFIILGCILESFINPSI
                     MSGILKLWK"
     gene            complement(8475..9503)
                     /locus_tag="PLC71_01565"
     CDS             complement(8475..9503)
                     /locus_tag="PLC71_01565"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRM01365.1"
                     /translation="MGMHKIAKMPSPEELKEEMPLSEEAKQIKAARDKEIRDVFTGAS
                     DKFLVIIGPCSADNETAVMDYLGRLARVQKDLADKLLIIPRIYTNKPRTTGDGYKGMV
                     HQPDPEKAPDMASGLRSVRKLHMEALEEFHMPAADEMLYPGNWPYMEDLLSYVAVGAR
                     SVENQQHRLTVSGFDIPAGMKNPTGGDLTVMMNSITAGQHQHDFIANGYEVKTDGNPL
                     THAILRGSVNRHGNNIPNYHYEDLTRVAYMYEKFQLKNPAVIVDANHSNSGKQWDQQP
                     RIVQEVLHSRSFLPEIKSLVKGIMIESYIEDGNQSIGNGVYGQSITDPCLGWDKSAAL
                     LYRMADYI"
     regulatory      9702..9844
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            9992..10609
                     /locus_tag="PLC71_01570"
     CDS             9992..10609
                     /locus_tag="PLC71_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM01366.1"
                     /translation="MAGDTMTMKRTKFNIRTIAVTGMLGALATVLMFLEFPIPMLIPP
                     FIKFDFSELPALLAAYAMGPVSGIAVCFIKNAINLLHTQTGGVGELSNFILGVCFVLP
                     AGLIYKRKKTQKSAMIGALAGAVLMSVVSVFSNYFVVYPVYTNFMPMQAILGAYQAIN
                     KNVKTLWDALIWFNMPFTFIKGMCSVVITFFIYKKISPILKGTNR"
     gene            complement(10673..11065)
                     /locus_tag="PLC71_01575"
     CDS             complement(10673..11065)
                     /locus_tag="PLC71_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRM01367.1"
                     /translation="MNIHVGDEIELTEEVVPENTAEKWGSGSLPVYATPAMISLIEQS
                     AVNLLAGKLEEGQTTVGTNLNIAHVSATPVGGTIKCHCSLTEIDRKRLVFHIEVTDNK
                     GRVGIGTHERFIVASEPFMEKAAKKLED"
     gene            11223..11852
                     /locus_tag="PLC71_01580"
     CDS             11223..11852
                     /locus_tag="PLC71_01580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRM01368.1"
                     /translation="MLGLKLYLIRHGETDWNKEFKIQGSSDIELNEYGRELAFITREG
                     LRHIPFDIAYTSPLKRAKETAEIILGGRNIPLYEDKRVQEACFGSFEGATLKELRERQ
                     DPFLKFFDAPEEFKKIEGCETHEEVIARSSDFFNDMILPNEKKYQHIAVFSHGAWIHS
                     LLTYIYQREIKEYWHAPRQENCGVTTIEIEDGVCHVLKESEIFYNKDGE"
     gene            11856..12314
                     /locus_tag="PLC71_01585"
     CDS             11856..12314
                     /locus_tag="PLC71_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HRM01369.1"
                     /translation="MTKRQFMEELRSSLEGMVSQAVIQENMNYYEDYINEQIRNGKNE
                     QDVLNELGSPRLIARSIIDAKGEDDDYVQTEYYEEEQDEGTPEDMFGGNTHIFTMNST
                     KFKLGCLLSVIIFIFIIYLLFHVFSAMMVVLGPVIIIGVIVALIMKITGR"
     gene            12337..13473
                     /locus_tag="PLC71_01590"
     CDS             12337..13473
                     /locus_tag="PLC71_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HRM01370.1"
                     /translation="MDINEEAYAIQEEVVEFRRALHRCPEIAFHETMTMEYIREHLEE
                     WEISYKIFDPSGIIAVVGNGKKETIALRADMDALEVQEETGLDFASLNHGCMHACGHD
                     GHTAILLATAKILKKHESELDRRVYLVFQPAEETAEGARFMLKTGVLDSVKRIYGVHI
                     FNGIPSGKISLEAGPRMAATNWLAVHLYGRSGHAGKPHEGIDTAVAVSSMVMNFQSII
                     SRNLNPLDPAVLTIGKVEAGTARNVIAGEAKIEGTARTFSRQAQEMIEHRVKEIAKAH
                     EQMYGVDVSVDFQQSSHGALINDPGVVEDVMEKAGEVFDPSEFTHVEAMMLGEDFANY
                     LEEMDGCFAFIGGGDHPANHHGKFDFDEKALISGVRLMLTFVIV"
     gene            13574..15064
                     /gene="thrC"
                     /locus_tag="PLC71_01595"
     CDS             13574..15064
                     /gene="thrC"
                     /locus_tag="PLC71_01595"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRM01371.1"
                     /translation="MNLVYHSTRNSEETATASEAILKGLTSDGGLFVPDSIPKLNVSL
                     EDLTKMSYQEIAYAVMKEFLTDFTEEELKTCINNAYDSKFDTEEIAVTKKVDGAYYLE
                     LFHGATIAFKDMALSILPHLLVTSARKNNVKNEIVILTATSGDTGKAALAGFADVPGT
                     KIIVFYPKSGVSPIQEKQMVTQKGDNTYVIGIKGNFDDAQTGVKKMFSNKELAKVMND
                     NGFQFSSANSINIGRLVPQVVYYVKAYADLLKQGALKAGEPMNVVVPTGNFGNILASY
                     YAKQMGIPIGKFVCASNKNKVLFDFFETGKYDRNREFYVTTSPSMDILISSNLERMIY
                     RIAGNDAKQCAKFMEALTKDGEYVITDAMKAELSEFFGAFGSEEETAVKIKEVYDKEG
                     YVMDTHTAVAAVAYDKYKAATGDDKTPTVIASTASPYKFTRSVMDAIDPAYDAEDDFE
                     LVDELNKVSKTAIPKAIEEIRTAPVLHDTVCETAAMEDEVKKILGI"
BASE COUNT         5425 a         2508 c         3562 g         4084 t
ORIGIN      
        1 tgagagtcta cattatctcg atgtgagtat gtttaatcac attttgagta gcagacagac
       61 atgataccaa aagacccagt gatgttatta agctacatca atacacaatt acgagatttt
      121 tatgattcat atgaagacct ttgtaagtca ctcgatcttg atcaaaatga agtaaaacaa
      181 aagctttcca tgatcggata cgaatatgat gaatcattaa atcagtttaa gtagcagact
      241 agtcgaatca atgaaaatat gttaaaattg tacgtaaagg agaacatatt tatggagttg
      301 attcggttag aaaatgtaaa gaaaatatat cagacaggag aggtgatcac caaagccgta
      361 gatgggatcg attttacggt ggatcagggt gagttcgcct tgatcattgg cgccagtggt
      421 gcaggaaaaa ctaccgtttt gaacatttta ggaggtatgg atactgcgac cagtggcgct
      481 gtttatattg atggagaaga tatctccaaa tggaataaaa aacaactgac aggatatcga
      541 agagatgaca taggatttgt atttcagttt tacaatctgg tgcccaatct tactgcaaca
      601 gagaatgtag aattggcact tcagatcagt aagaatcctc tgaaagcaga agaagtatta
      661 aaagatgtgg gacttggaca ccgtctgaat aattttcctg cgcagttatc tggtggggaa
      721 cagcagagag tttctattgc acgtgcattg gcaaagaatc caaagatctt gctctgtgat
      781 gagccaactg gtgccctgga ttatcagaca ggaaaatcta ttttaaaatt attgcaggat
      841 acctgtcaca agaaaggaaa aacagtggtt gtgatcacac ataaccttgc gatcgcaccg
      901 atggcagacc gtgtgatcga gatgaagaac ggaaagatca gcaatattac gataaacgaa
      961 caccctcagc cggtggagac gattgaatgg taggaaatgc gtatgaagaa aacggcatta
     1021 cataaagata tatacagaac gatcacagga acccttccga gatttttatc aatctttttt
     1081 atcgttgtac tgggtgttgc attctattca ggaatccgtg tgacacaaaa agatatgcat
     1141 atcacagcag accaccagtt tgatggaagt cggctgatgg atgtcaaagt catgggaaca
     1201 cttgggattt cagaagagaa tctgacagcc cttcggaagt taaaaaatgt gcagtcggtc
     1261 gaaggaggac attctgtaga tgtgatcgca aggaagcagg gaagtgacga agaacatgca
     1321 atcaaagtta tggggaagac tgataaatta aaccagataa cgatcataga tggaaaactt
     1381 ccacaagcat cgggagaatg tctggtagat gaatggtatg cccagcaaaa tgatctgaaa
     1441 acaggggacg ttttaaattt gtcttctgga aatgaagatg atcttaagga tactctgaaa
     1501 gatacaacct ataagatcac aggaatcgga agttcatcag aatatctttc aagatccaga
     1561 ggaagcactg gcattggaac gggaacgttg tcaggcttta ttgtggtgca accatcagaa
     1621 ttttcctcag atatctatac agaagtttat ctgactgcga agggagcgaa gcaagagaga
     1681 gcttacagtg atgcttacaa aaacaaagta aaacagctgg aagaagagat caaagatatt
     1741 tccaaaatag aaaatgagaa acggttaaga tctgttcaga aagaagccga agagaagatc
     1801 gacaagaaag aaaagaaatt caaaaaagaa cgaaaaaaag catataagaa actagatcaa
     1861 gccgagaaaa agctaaagaa atccagacaa aagatcaaag attccaaagc aaaattagcg
     1921 aaaagcaaga aagagatctc tgatggagag cagcagatca agaatgcaaa agctcagtat
     1981 cagtcaggga tcagacagat tcagaatgcc agaaagcagt taaagacaca gaaacaaaca
     2041 attcggtcaa aaagcaaaga attaaaaaca caggaggcaa agctaaagac acaggagaag
     2101 aacttacaga caaaagaaca gcagattcag ggtgccatgc aggctggaat gatctcacag
     2161 gatgaagcga aagcttcctt agcacagatt aatgcaggaa aagaacagat caagacagca
     2221 agaagcaaga ttaatgcagg gaaaaaacag atcagcaggg caaagacaaa gattgcaaag
     2281 gctgaaacaa ccttggatca aaaaagcagc cagctaaaga aagcaaagca aacgatcaaa
     2341 acaaatgaag caaaattagt atcagcaaga aaacagatca cctccggaga aaaaaagatt
     2401 gcaaaagcag aatcaaaact ttcatccggg gagaaaaaat taaagagatc aagaaagaaa
     2461 gcagaaaaga aattcaagga agccgagcag aagattgcag atgccagaca ggatgttaag
     2521 gatatcaaga aagggaaatg gtatgtctta aaccgtgaga agatgcagtc ttatgtggaa
     2581 tacggacagg aagcagacag gattgcagcg ttaggacgag tggtaccggt catctttttc
     2641 ttagttgcag cactggtaag cttaacagcg atgacacgta tggtcgaaga gcagagaaca
     2701 cagatcggta tgatgaaagc attgggatac agtggagttc atattgcaat gaaatatgtg
     2761 acctatgccc tcgcagcaac gcttagtgga agtattctag gggcagtgat cggtgaaaaa
     2821 ctgcttccat ggatcatcat taacgcttat aaaatgatgt atacaggact gggagatgtc
     2881 tatacgccat tagagatcga atattctgtg atggcaggtg gactggcggt tggagttgtt
     2941 gttttagcag tattatctgc atgttataaa gaattaagag aaaaacctgc acagctgatg
     3001 cgtccagttg ctccaaaaga aggaaaaagg attttgttag aacggattcc atttatctgg
     3061 aaacgattaa gttttatctg gaaagcaacg atgcgaaacc tcttccgcta caagaagaga
     3121 ttctttatga cgatctttgg aatcggcgga tgtatggcat tattactctt aggatttgga
     3181 atcaaagatt cgatttcagc aatctcagaa aagcagtatg gggagatcat tacttatgat
     3241 ttctcgatca cacacaaaga cgggatcagc gagacaaaga aagaagatct gatccagtat
     3301 gcaaagaaac aagaacatat gacagatttg atagaagtaa gcgaatctgc agtgacgatc
     3361 cgtgctaatg ggaaaaaaca ggatgcaacg atgatccagc caatgtccaa aaaagatctg
     3421 aatggctata tcagccttca ggatcgaaag agtaaaacaa agtatcagtt agatgatgat
     3481 gggatcatct taacagaaaa ggcagcgtcc ctgcttggtg tgaaaaaagg agattcgatc
     3541 aagatccaac ggtctggaga taaacagatc acagcaaaaa taagtcagat cacagaaaat
     3601 tacatgcagc ataaagtcta catgacacca aaactttatg agaaactgta tcataaaaaa
     3661 gcacaaacga caggtatata ttgtattgaa aagaatatat ctaagaaaaa gatgcaggaa
     3721 aatggaaaac agattcttgc aagagatgaa gcctcaacac tgcatttttg tgccgacgat
     3781 gagaagacaa tgtcagatat ggtcaataac ttaaatattg tggtggttgt tctgatcgta
     3841 tctgcaggac ttcttgcatt tgtggtatta tataacttaa ataacattaa catttccgag
     3901 cgtagaatgg aacttgcaac gatcaaagtt cttggattct atgatggtga agtcctgcag
     3961 atacgatcan nnnnnnnnnn nngtgaagtc ggagcgtatg tgtatcgaga gaatatcctt
     4021 ttaacgatcc ttggaacgat cgcaggaatt attttaggaa ttattttaca caaatacgta
     4081 atcttcacaa cagaagttga cttgatcatg tttggaaggc agatttatgt gcaaagctat
     4141 gtttacagta ttttactaac aattgctttt tcgattctcg taaacttctt tatgtatttc
     4201 caactaaaga agattgatat ggtagaatcg ttaaaaagca cagagtgaca gaaaggaaaa
     4261 gagactatga aaatgaacga attaatgatt tatcaagtgt ttccactaag agcattaaca
     4321 gatgggcagc catctcatgg aattctcgag atgatagact ggattcaaca tattaaaaaa
     4381 ttaggaatga atgcagtata tttttcacct gtattttcat ccagtgatca tggatatgat
     4441 acaaaagatt atcagaagat cgatgagaga cttggaacca atgaagactt taaaaagatg
     4501 tgtgatgcca tgcatgaaga aggaatccag gtgatcttag acggggtatt taatcatgtc
     4561 ggaagagagt tctttgcatt tgaagatgtg agagaaaaga aatgggattc tccatataaa
     4621 gattggttct atattaattt tgatgggaat agctgctatg atgatggatt ctggtacgaa
     4681 ggatgggaag gacattttga actggtcaaa ttaaatcttg acaacgaaca ggttagaaat
     4741 tatttattag aatctgtcgg aatatggatc gataaattag ggattgatgg attaagactg
     4801 gatgttgctt atatgttaaa ccgcaatttc atgaaagtgt tggttgactt tgttcacagc
     4861 aagaaaccag aatttttgat gatcggagaa atgcttcatg gtgattacac acagcttgtg
     4921 aatccagagc tgttggattc tgtgacaaat tatgaatgtt ataaaggttt gtattcaagt
     4981 ttcaatacac acaatatgca tgagatcgga tattccctaa accgtcagtt tggaccagaa
     5041 gaatggacgt tgtacaaagg acttccatta tataattttg tagataacca tgatgtggag
     5101 agaatcgcaa gtcagttaga ggataaagag catttaccat tgatctatgc aatggaattt
     5161 gcgatgccag gagttccggg tgtttactat ggaagtgaat ggggaatgga aggaaagaaa
     5221 gaacagggaa gtgatgacag cctgcgtcca agaatccata aagaagattt agtagaaaat
     5281 gaattaacaa actatatcgc acaattggca aaagtaagag ctggatcacc agctttaaaa
     5341 tacggtgatt acttacaatt agcgatcgca ccagatgttc ttgtattcca gagaagaacg
     5401 aatgaagacc gcatcatttt tgcaatgaac tgtggtgatg gcgaatttac agcacatttt
     5461 gatgctcagg caggatgcgg gattgatctg atcacaggag attccgttga ttttggaggg
     5521 ggacttacga tccaaggaaa aacagcaatg ataattcgaa cagaagaggt atcccttcct
     5581 tgacttttat gttaaaaaca tataaaatta aatgagtgtg aatacacaaa aaattgaata
     5641 aggaggaaac ctgtggttgg tattattata gccattatcg caactctggt tatcgcagtt
     5701 ccggtatctg cctatgtagc gaatacaaga agcaagaagg caatggaaat tacgatcggg
     5761 aatgcagaag ataaagcaag agagattata gatgaagcaa tcaaagcagc agatgcgaag
     5821 aaacgtgaag cttctttgga agtaaaagaa gaaactataa aagcgagaca tgacttcgaa
     5881 aaagagacaa aagaacgcag ggcagaactg cagaagtatg agcagagagt tctttctaag
     5941 gaagagactg tcgagaagaa agcaaccgtg ctggaacgca aagaacagtc attaacagcc
     6001 aaagaaaaga acatcgatac agagaaagct cagttgcagg aactgcaggc aaaacatcta
     6061 caggaactgg aaagaatttc tgggttgacc tctgatcagg caaaagagca attattatca
     6121 gcagtaaaag aagatgtgaa acacgagaca gcaatgtatg tcaaagaaat ggagacaaga
     6181 gcgaaagaag atgcccgaaa gaaagccaag gaatatgtgg taactgcaat tcagaagtgt
     6241 gcagttgatc atgtggcaga gacaacgatt tcgctggtac agcttccaaa cgatgagatg
     6301 aaaggaagga tcatcggtcg tgaaggacgt aatatccgtt ccattgagaa cgctacggga
     6361 gttgaattga tcatcgatga tacaccggaa gctgttgtac tgtcaagctt taatccggtt
     6421 cgacgcgaga tcgcaagaat cgctttagag aaattgatcg tggatggaag aatccatccg
     6481 gcacgcatcg aggagatggt tgagaaagct acaaaagaag tcgaaactat gatgcgcgaa
     6541 gaaggggaag cagcaacatt agaacttggg gttcatggaa tccatccaga acttgtcaga
     6601 cttcttggaa gaatgaagtt ccgtacaagc tatggacaga atgcgttaaa gcattccatg
     6661 gaagttgctc agttatccgg acttttagca gcagagatcg gtgtcgatgt gagaatggcc
     6721 aagagagcag gtctgcttca tgacattggt aaatccatcg accacgaagt agaaggttct
     6781 cacgtagaac ttggtgtgaa tctgtgtaag aagtataagg agaatccaat tgtcatcaat
     6841 gcagtggcat ctcatcatgg ggatgaagag ccagaatcat tgatcgcatg tctggtacag
     6901 gcagctgatg caatctcagc agcacgtcca ggtgcaagaa gcgagacact ggaaagttat
     6961 acaacaagat taaaacagct ggaagagatc gcagactcct tccagggtgt tgataaaact
     7021 tttgcaattc aggcaggtcg agaaattcga gttatggtag ttcctgaaca aatcagtgac
     7081 gatgatatga tcttactggc gagagatatc tcaaaccaga tcgaagagaa tctggattat
     7141 ccaggacaga tcaaggtcaa tgtgatcaga gagtcacgag ttgttgacta tgcgaaataa
     7201 caagaagcga tagagaaatc tatcgctttt tccaatttat aacgtgagtg tcaaaagtat
     7261 aaataaagga gattttttat gaaaggatac gagaaactag aacaacatct gatacatcag
     7321 ggaaaagttg ttggatttta tgaggatatt gtaaaacttc caagcggcaa agttgtcaca
     7381 tgggatctgg tcaaacataa aggggcagca gctgttgttc ctgttacaga caaaggaacg
     7441 atcttacttg taagacaata ccgtaatgcc cttgatcagg aaacactgga aatcccggca
     7501 ggtggaattg aacccgggga aacacctctg gagtgtgtca caagagagat tgaagaagaa
     7561 acaggtttta ttgctggaaa aatgacccac ctaatgactg tgatcacagc catcggtttc
     7621 tgtgatgaaa agattccgat ttatgttgca actgatctaa agctgtctaa acagcatttg
     7681 gatgaggatg aatttatcga tgtagaagaa tatacaatcg atgagatcaa agatatgatc
     7741 ttttctggaa agatcattga tgcaaaaaca atttcaggag ttcttggata tctgaatatg
     7801 gagaaataaa agactagatc aggaaaacag aatataaaag acaggcactg cataagatat
     7861 gacaggagga aaaaattgtg gaaaaagaga aattcttttc aggattatgg atcatcattg
     7921 ggttaatctt nnnnnnnnnn tgggagtgta tttacgaatt ttgtatacca ggattatctc
     7981 gcatatggag gtaccgtaag atcactggtt ttaaataata cggatctata taaagcaagt
     8041 atcagtctgt ttttttatat tttgtttaaa aggggaaaac aatatgcatt tatttatctg
     8101 ggggcatatc ttttgcaacc ttggattttt ttatacagtt ttgcattttg tatggcattt
     8161 ttctttggat caatgttatc tttgcagatc gtgcaaatgg gcatcaaagg actggttctt
     8221 gtatttatga gtttatttcc acattttatc tgttatgtga tcaccttgct gctgctgatc
     8281 aagcgaaact tttatgccca aaaaaaagaa gaaatgctct atgaacagag acattccttc
     8341 ttatttcgtt tcagtatttg gtttgaaata acatttataa tactgggatg catcctggaa
     8401 agtttcatca atccgtcgat catgtcagga attctgaagc tttggaagtg atatatctag
     8461 gatcttatac gtttctatat ataatctgcc atgcggtaaa gcagagcagc agatttgtcc
     8521 catccaagac aaggatcagt gatagactgt ccatatacac cgttaccgat ggactggttg
     8581 ccatcttcga tataactttc gatcatgata cccttaacaa gagatttgat ctcaggtaag
     8641 aaacttctgc tgtgaagaac ttcctgtacg atacgaggct gctgatccca ttgtttccca
     8701 gagttagagt ggtttgcatc aacgataaca gcagggttct ttaactggaa tttctcgtac
     8761 atataagcaa cacgagtcag atcttcataa tggtagtttg gaatattgtt tccatgacgg
     8821 ttgacagatc cacgtaagat ggcatgtgtc agagggttac catctgtctt gacttcatat
     8881 ccatttgcaa tgaaatcatg ctggtgctgt ccagctgtga tagagttcat cataacagtt
     8941 aagtcaccac cagttgggtt cttcattccg gcaggaatat caaatccact aactgttaaa
     9001 cgatgctgct ggttctctac agaacgagct ccaacggcaa cataagataa aagatcttcc
     9061 atatatggcc agtttcctgg ataaagcatt tcgtctgcag caggcatatg gaattcttct
     9121 aatgcttcca tatgaagctt gcggacagaa cgaagtccac tagccatatc tggtgctttc
     9181 tcagggtctg gctgatgaac catacctttg tatccgtcac ctgttgtacg aggtttgttt
     9241 gtataaatac gagggatgat cagtaactta tcagcaagat ctttctgaac tcttgctaaa
     9301 cgtccgagat aatccataac agcagtttca ttatctgcag aacatggccc gatgataact
     9361 aagaatttat cagatgcgcc tgtgaataca tcacggattt ctttgtctct tgcagctttg
     9421 atttgttttg cttcttcact cagaggcatc tcttctttta gttcctctgg agaaggcatt
     9481 ttagcgattt tgtgcattcc catagttgtt ttctccttaa caattcatac aataaagcaa
     9541 atttgcttgt gtatatttta acaaataata atctttgtgt aaagataagg aaatgtgaaa
     9601 tgggacttta caaaacagac aggagttatt ataataaaat ttataaacag gaaacacttt
     9661 cattttctgg taattgaata ttgaagtaga gaagattgat tctccaactt cagggcagag
     9721 tgagatacga aagtatcaat tctcgactgg cggtgatact ccaagagagg caagtccgcg
     9781 agcgtttggc cgatcaggtt agaatcctga accaacagta tagtctggat gaaagaagta
     9841 gttggatgac atatcttaag atgttatgca gatagaagat atgtgatcaa gtcagaagcc
     9901 ctgtcgttta tgtagataaa tggacaggat tttttaattt atagggagac cctataaatc
     9961 taagatttta aatcagaaag ggagaattat catggcagga gacacaatga caatgaagag
    10021 aacgaagttc aatatcagaa cgatcgcagt gacaggtatg ttaggagcat tagcaactgt
    10081 gctgatgttt ttggaatttc caattccaat gttgatacca ccatttatca agtttgactt
    10141 ttcagaactt ccagcattac tggcagcata tgcgatggga ccggtatctg gaattgcagt
    10201 atgttttatc aagaatgcga tcaaccttct tcatacacag acaggtggag tcggagaact
    10261 atctaacttt atcttaggtg tatgctttgt attaccagca ggacttattt ataagagaaa
    10321 gaaaacacag aagagtgcaa tgatcggagc attagcagga gcagtcctga tgtcagtagt
    10381 cagtgtattt tcaaactact ttgttgtata tccagtatac acaaacttca tgccaatgca
    10441 ggcaattctt ggagcatatc aggcgatcaa caagaatgta aagacattat gggatgcatt
    10501 gatctggttt aatatgccat ttacatttat caaaggaatg tgcagtgttg tgatcacatt
    10561 ctttatctac aagaagattt ctccaatctt aaagggaaca aacagataag aagataagaa
    10621 aaaggtgtcg gaataagaat tccggcacct tttgcgtaca cagagttaaa aattaatctt
    10681 ctaatttttt agctgctttt tccataaaag gttcactggc aacgatgaat ctttcgtgag
    10741 taccgattcc aacacgtcct ttgttatcag ttacttctat atggaatacc agacgtttgc
    10801 ggtcgatctc tgttaaggaa caatgacatt tgatcgttcc accaactggt gttgcggaaa
    10861 catgcgcaat gtttaaattt gttccaacag tagtctgtcc ttcctcaagt tttccagcta
    10921 gtagattaac tgcagactgc tcgatcaaag agatcatggc aggtgtggca taaacaggaa
    10981 ggcttccgct tccccatttc tcagcagtgt tttctggaac tacttcttct gttaattcga
    11041 tttcgtctcc aacatgaatg ttcataattt tctctcctaa aattttagtt tatatgtata
    11101 tctttagtat agcaaagttc gttaaaaatt ccatagtttt ttcacatttt tacaaaaaat
    11161 tgcacaagga agaaagactg gactcgattt aaaaaataag gtatgctatt ggtaaattgg
    11221 aggtgcttgg tttgaaatta tatttgatca gacatggaga aacagattgg aacaaggaat
    11281 ttaagattca gggaagttca gacatcgaat tgaatgaata tggaagagaa ttggcattta
    11341 ttacgcgaga aggtcttaga catattccat ttgatatcgc atataccagt ccgttgaaac
    11401 gtgcgaagga aacagcagag atcattcttg gaggcaggaa tattccgcta tatgaagata
    11461 aacgggttca agaagcgtgt tttggatcgt ttgagggtgc aacgttaaag gaattgagag
    11521 aaagacagga tccatttctc aaattctttg atgcgccaga agaatttaaa aaaattgaag
    11581 gatgtgagac acacgaagag gtgatcgcaa gatcatctga ttttttcaat gatatgatct
    11641 tgccaaatga gaaaaaatat caacatatag cagtattttc tcatggagca tggatccaca
    11701 gcttacttac atatatttat cagagagaga ttaaggaata ctggcatgcg ccaagacagg
    11761 aaaactgtgg tgtgacaacg atcgagattg aagatggtgt ctgccatgta ttaaaggaat
    11821 cagagatttt ctacaataaa gatggagaat gatatatgac aaaaagacaa tttatggagg
    11881 agttaagatc ttccttagag ggaatggtat cacaggcagt gattcaggag aacatgaatt
    11941 actatgagga ttatatcaat gagcagatcc gaaatggaaa gaacgaacag gatgtattga
    12001 atgaattagg aagtccgaga ctgatcgcca gatcgatcat tgatgccaaa ggtgaggatg
    12061 atgactatgt acagacagag tattacgaag aggaacagga tgaaggaaca ccagaagata
    12121 tgtttggtgg taacacacat atttttacga tgaattctac aaagttcaaa cttggatgtc
    12181 ttctgtcagt gatcatattt atttttatca tttatttact atttcatgta tttagtgcaa
    12241 tgatggttgt gttaggcccc gtgatcatta tcggtgtgat cgtagctttg atcatgaaga
    12301 tcacaggaag gtaaggaaac aaagaaagga atcaatatgg atataaatga agaagcatat
    12361 gcgatacagg aagaagtggt cgaatttcga agagcactgc accgttgtcc tgagatagca
    12421 tttcatgaaa cgatgacgat ggaatatatc agagaacatc tggaagaatg ggagatttct
    12481 tataagattt ttgatccatc aggaatcatt gcagttgttg gcaatggcaa gaaagaaacg
    12541 atcgcattaa gggcagacat ggacgcactg gaagtacagg aagagacagg acttgatttt
    12601 gcatctttga atcacggatg tatgcatgca tgtggacatg atggacacac agcaatctta
    12661 cttgcaacag caaagatatt gaagaaacat gaatcagaac ttgaccgcag agtatatctt
    12721 gtatttcagc ctgcggaaga aaccgcagaa ggagccagat tcatgttgaa gacaggagtt
    12781 ttggacagtg tgaaacgcat ttatggagtc catattttta acggtatacc gtctggcaag
    12841 atttcactgg aagcaggccc aagaatggca gcgacaaact ggctggcagt tcatttatac
    12901 ggaagatcag gacatgcagg gaaaccacat gaagggattg atactgcagt ggcagtatct
    12961 tccatggtta tgaattttca gagcatcatc agcaggaatt taaatccatt agatccagca
    13021 gttttaacga tcggaaaggt cgaagccggg acagcgagaa atgtgatcgc aggggaagca
    13081 aagatcgaag ggactgcaag aacattttcc agacaagctc aggagatgat cgagcacaga
    13141 gtcaaagaga tcgcgaaagc ccatgaacag atgtatggag tagatgtttc ggttgatttt
    13201 caacaatcaa gccatggggc actgatcaat gatccgggtg ttgtagaaga tgtcatggag
    13261 aaagcagggg aagtctttga tccgtcagag tttacccatg ttgaagcaat gatgttagga
    13321 gaagattttg caaattatct ggaagaaatg gatggatgtt ttgcatttat cggtggcggc
    13381 gaccaccctg caaaccatca tggaaagttt gattttgatg aaaaagcatt gatcagtggt
    13441 gtccgtctca tgctgacatt tgtaattgta taagatatag ttcatgaaaa tcagttgtag
    13501 acaggaacaa catatgatat aatcaaaaac caacagaaac acgtatattt aatagaagaa
    13561 aaggtaggga aatatgaatt tagtatacca cagtacaaga aatagtgaag aaacagcaac
    13621 cgcctctgaa gctattttaa aaggattaac aagcgatgga ggattatttg taccagacag
    13681 cattccaaaa ttaaatgtat cgttggaaga tctgacaaag atgtcctatc aggaaattgc
    13741 ttatgcagtc atgaaagaat tccttacaga ttttacagaa gaagaattaa agacatgcat
    13801 taataatgca tatgacagca aatttgatac agaagagatc gcagtgacaa agaaagtaga
    13861 tggagcatat tatctggaat tattccatgg agcaaccatc gcatttaaag atatggcatt
    13921 atctattctg ccacatttac ttgtaacatc agcaagaaag aataatgtaa agaatgagat
    13981 cgtgatctta acagcaacat ccggagacac aggaaaagcg gctttagcag gatttgcaga
    14041 tgtaccggga acaaagatca tcgtattcta tccaaagtct ggagtcagcc caattcagga
    14101 aaaacagatg gtaacacaga agggtgacaa tacatacgtg atcggaatca aaggaaactt
    14161 tgatgatgct cagacaggtg ttaagaagat gttctctaac aaagagttag cgaaagtgat
    14221 gaatgacaac ggattccagt tctcatctgc aaactcaatc aacatcggac gtttagtgcc
    14281 acaggttgta tattatgtaa aggcttatgc tgatttatta aaacagggtg cattaaaggc
    14341 cggagaacct atgaatgtag tcgttccaac aggaaacttt ggtaatattt tagcttctta
    14401 ttatgcaaaa cagatgggta ttccaatcgg caaatttgtc tgcgcatcta ataagaataa
    14461 agtattattt gatttctttg agacaggaaa atacgatcgt aacagagaat tctatgttac
    14521 aacatctcca tctatggata ttctgatctc aagtaactta gagcgtatga tctatagaat
    14581 cgcaggaaat gatgcaaaac agtgtgccaa atttatggaa gcattgacaa aagacggtga
    14641 atatgtgatc acagatgcca tgaaagcaga attatctgag ttctttggag ctttcggaag
    14701 tgaagaagaa acagcagtga agatcaaaga agtctatgac aaagaaggat atgtgatgga
    14761 tacacataca gcagtcgcag cagttgcata tgacaaatat aaagcagcga caggagatga
    14821 caagactcca acagtgatcg cttctacagc aagtccatac aaattcacaa gaagtgttat
    14881 ggatgcgatc gatccagcat atgatgcaga agatgacttt gaattagttg atgaacttaa
    14941 caaagtatct aagacagcaa ttccaaaagc gatcgaagag atcagaactg caccagttct
    15001 tcatgatacg gtatgtgaga cagcagcaat ggaagatgaa gttaagaaga ttcttggaat
    15061 ctaatgaatc ataaaaaagc aacccatgga agaaaatggg ttgctttttt tatgcgaaaa
    15121 tgttatgata aaccaagata gggaataaat ggaagagaat acaatatcag ataattataa
    15181 aaacatatca acaagataaa tctaagatga gatcaatcag atctgacaaa tccattcacg
    15241 acatatgatg atcgaatgat gatgaatcca agggaagctg acaattgaat aagaccattt
    15301 tccggcacag tccgggagga catattccgc catgatctgc gggcaatgaa ttttatgcca
    15361 tgaacaatag gattgaggtt cttttatgat accacgaaga gcacagggag cgaacaagga
    15421 tatgataatg agacttctgc atggtcatgg aggtgagtgc agcctgtcat aagaatgagg
    15481 ggagccggga ggctatgaga cattttgtct gttggagaat gagggaaata taaagatatg
    15541 acaaggagga gagtacatta tgaagaaaaa gagacaatgg ttcatgtgcc ttgggatgat
    15601 cg
//
LOCUS       DAOMUE010000015        15421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_790_length_15421_cov_2.625081, whole genome shotgun sequence.
ACCESSION   DAOMUE010000015 DAOMUE010000000
VERSION     DAOMUE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 15421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15421
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_790_length_15421_cov_2.625081"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..>610)
                     /locus_tag="PLC71_01600"
     CDS             complement(49..>610)
                     /locus_tag="PLC71_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRM01372.1"
                     /translation="VGIDRYLGEHIPVVSCDNYAAGQMATRHLIDKGCQHILCIRGNS
                     KLKMPGNNRSQAYIQEMEKVGLPQMILEVPFIMENVEKQKLIYDMLNAHPEIDGIFAG
                     DDSLAVIALHVARQKGINIPKDLKIIGVDGTKQILGFVPELTTIQQPVKQIAKVAVDK
                     LIDLIEGKTAESCMDLPVKLLEGQTT"
     assembly_gap    611..620
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<621..1013)
                     /locus_tag="PLC71_01605"
     CDS             complement(<621..1013)
                     /locus_tag="PLC71_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM01373.1"
                     /translation="MKPGIKDVAKVAGVSPTTVSRVLNNRGYISEETRKKVYDAMEEI
                     NYYPNEIARALLNNRTYFVGVIVPTVTSPFHGEVVEQIEYYLSQKNYKMLLCNSKNQM
                     DTEKAYIDMLRRNQVDGMIVGTHNAVVET"
     gene            complement(1041..1547)
                     /locus_tag="PLC71_01610"
     CDS             complement(1041..1547)
                     /locus_tag="PLC71_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRM01374.1"
                     /translation="MNYKQAKIADSAKVAKETVLVGNITIGEESTVLFFTAMRCEGEE
                     SIVIGNQSNIQENCTIHVDEGNSVKIGDGVTVGHNSVIHGCQIGDNSMIGMGSVVMNG
                     AKIGNHCLIGAGSLVTQNTVIPDRSLVMGSPARVKRTLTEDEIKYLEGSREEYVEVGK
                     LLREDGIL"
     gene            complement(1551..2042)
                     /locus_tag="PLC71_01615"
     CDS             complement(1551..2042)
                     /locus_tag="PLC71_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01375.1"
                     /translation="MKKEKVTTNKVTSKLEKVLWAVLILMFIVSLIGMFLGQYMDGNV
                     FYKMCVIMTVLAAAILSAERFYFGKEMGRDMPEAFKIGYWLIIAWAVILLIHAAGLAF
                     LPFLMELEYRVLWVVVPTVSLLWMTRYIFGDNLDWDKTDKKGWKEGRMNYSQKSNKKN
                     IGR"
     gene            complement(2054..2689)
                     /locus_tag="PLC71_01620"
     CDS             complement(2054..2689)
                     /locus_tag="PLC71_01620"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRM01376.1"
                     /translation="MRVLILTGGTVEENFAKDYISKWNPDQVITADKGLLYAKRLNIK
                     PDIILGDFDSCSKDIMQEFSTDKKIIVPCEKDDTDTGLAIQKAIETGADEILMIGGTG
                     TRLDHVMGNFGQLFYAHSKGVKAELVDANNRIRVLNHEDTISKKDQFGKYVSLIPIYE
                     ARGVTLTGFKYPLKDHTLVFEQSLAISNELEAEEGIISFSEGKLLLIESRD"
     gene            complement(2689..3564)
                     /gene="rsgA"
                     /locus_tag="PLC71_01625"
     CDS             complement(2689..3564)
                     /gene="rsgA"
                     /locus_tag="PLC71_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRM01377.1"
                     /translation="MQGKIIKGIAGFYYVQTEDKLYECKAKGIFRNKKMKPLVGDNVE
                     IDILDETEQEGNITKIYERHNELIRPAVSNVDQALIIFAAKNPKPNYNLLDRFLMMME
                     KQDVPVIVCFNKVDLAKEKELKLLEKVYENCGHIVRFISVKEEEGIDEIRELIHGKTT
                     VLAGPSGVGKSSLMNLLQPEAQMETGEVSEKIKRGRHTTRHSELIRIEKDTYVLDTPG
                     FSSLFIHMFEEDEIKDYFQEFAPYEDECRFQGCSHTHEPDCGVKKALEEGKISKIRYD
                     NYVNIYTELKEKRKW"
     gene            complement(3592..5433)
                     /gene="pknB"
                     /locus_tag="PLC71_01630"
     CDS             complement(3592..5433)
                     /gene="pknB"
                     /locus_tag="PLC71_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HRM01378.1"
                     /translation="MLEIGTMLSGRYEVLKRVGSGGMADVYMAKDHKLNRNVAVKVLK
                     SEYVEDEKFLKKFETEAQAVARLSHPNIVNVYDVGIEDGINYIVMELAEGMTLKEYIR
                     RKGYLPPKETVEISLQIASAISHAHKNHIIHRDIKPQNILVSEIGNVKVTDFGIAKAT
                     SSNTVTSTATAMGSVHYISPEQTKGRFCDEKSDIYSLGITMYEMVTGHVPFDHENGVT
                     IALMHLQNEITPPSQIRDGIPDSLEKIILKCTMKKPEDRYQTADELIADLKLVFEDTS
                     GEYVGIVPTIDDSPTIMIDQNELTQRIQTNDDTQPVEDSDSNAYLEEDDEEEETGMNS
                     KIEKLVIVLAAVVGAIILISIVVFVVKSSGLFKSGKSTTQSTIAATTEAKHHNTTEKY
                     TVDNYIGLSLEAAREAIDNKFKINVTEEYSDTYAKGLVMQQDPESDTELEEGKTIKLV
                     VSKGQKIEDKVSVPDVRSKTKSGAQSELKAAGLKVSIKEAYSDDVAKGEVISQTPSHG
                     SKVSKNTTVVITVSKGKKEDQTVSVPNLRYYTESEASQELQRFGLSLGSVLTEYSDSV
                     EKGLVIRQRISSGSKVKKGTAVGIYVSLGPRQTATTADEESTTTDEE"
     gene            complement(5446..6165)
                     /locus_tag="PLC71_01635"
     CDS             complement(5446..6165)
                     /locus_tag="PLC71_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRM01379.1"
                     /translation="MRSVGASHIGKVRTENQDRIFFHDQPVGNLKNLYIVADGMGGHR
                     AGGHASSFAVERFVQLIEESKQTDPIELMKSSIASVNQEMIAESQSNEEYRGMGTTFV
                     AATISDGKVTVMNIGDSRLYYGNQELTQITVDHSYVEELIHAGIMEREEGRFHPKKNV
                     ITRALGTVMATPDFFEFDAKDGYLLLCSDGLSNMISDEELKELLLEHSQIANKVNQMI
                     TRANEYGGKDNISLVIVDLKR"
     gene            complement(6177..7208)
                     /gene="rlmN"
                     /locus_tag="PLC71_01640"
     CDS             complement(6177..7208)
                     /gene="rlmN"
                     /locus_tag="PLC71_01640"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRM01380.1"
                     /translation="MDLKSMTLQEMQEYMESIGEKKFRAKQIYEWFHKHLALSLDEMN
                     NVPKKLKEKIEQTQEIYGVKPVDCFISKIDGTRKYLFELYDGNIIESVLMKYKHGNSV
                     CISSQAGCRMGCKFCASTLGGLDRNLTPSEMLSQIYYIQRDTEERVSNVVMMGTGEPM
                     DNYDNVLRFLELITSEDGLNISQRNITISTCGIVPKIKELAQKHLQITLAISLHSPND
                     EMRRGLMPIAMKYSIDELLDACHYYFKETNRRMTFEYSLVAGVNDQPVHAEELAGRLK
                     GFPCHVNLIPVNPIKERDFKQSMPKSVMEFKKILEKNRVNVTIRREMGADINAACGQL
                     RRKKLQSRL"
     gene            complement(7213..8556)
                     /gene="rsmB"
                     /locus_tag="PLC71_01645"
     CDS             complement(7213..8556)
                     /gene="rsmB"
                     /locus_tag="PLC71_01645"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRM01381.1"
                     /translation="MQNPRECALDVLIKVDKKEELSHIAISNVLEKYQFSEKRDRAFF
                     TRLVEGTLERQITIDYVADQFSKTKIRKCKPLIRALLRMGIYQILYMDQVPDSAACNE
                     AVKLAKKRGFSRLSGFVNGVLRNISRKKDEIQYPSREKNLETYLSVAYSIPEWIIKFF
                     QKTYDNETVEKIIASYLEERKTTLCCLISKGGKEAVRKELEAEGVTVEDGSLIENAVQ
                     ISDYDFLYRLKAFREGSCYVQDESSMLCAKLASVQKEQFVMDLCSAPGGKSLYVADQL
                     KGSGRVLSRDLTEYKTDLIEDNIDRVGFTNMESEVFDARVLDEEHIEAADIVIADVPC
                     SGLGIMGKKNDIKYHISEEGMKDLVKLQREILANAVQYVKHGGTLIYSTCTINPAENE
                     ENFRWILDHFDFEAVDITKELPKDLKIETAQEGFIQLLPGIHPCDGFFIGKLRRK"
     gene            complement(8561..9490)
                     /gene="fmt"
                     /locus_tag="PLC71_01650"
     CDS             complement(8561..9490)
                     /gene="fmt"
                     /locus_tag="PLC71_01650"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRM01382.1"
                     /translation="MKAVFMGTPEFAVPTLQALIDHHEVLAVVTQPDKQRGRGKKMQF
                     PPVKEKAVEYDIPVYQPQRARDEEFIEELKNLNPDVIVVVAYGQILPESILNIPKYGC
                     INVHGSLLPKYRGAAPIQWAVLDGEEKTGITTMYMEKGLDTGDMIDKAEVVLDKKETA
                     GSLHDKLMVLGANLLLETLKKLEDGTAVREKQNDEESCYAKMLSKDMGQIDFTKSARE
                     IECLIRGMNPWPSAYTSLDGKTMKVWEADVLDAQSKEEPGTIVEVNKKEIIVATGSND
                     LALHEIQLAGKKRMQVQAFLLGYQVEAGTKLGE"
     gene            complement(9492..9971)
                     /gene="def"
                     /locus_tag="PLC71_01655"
     CDS             complement(9492..9971)
                     /gene="def"
                     /locus_tag="PLC71_01655"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRM01383.1"
                     /translation="MAIRQIRVLGDDVLRKKCKEVKEMTPRMHTLVEDMYDTMYEAMG
                     VGLAAPQVGILKRIVVIDTGEEGECVTLVNPVITLKEGEQVGEEGCLSLPGKVAVVKR
                     PDHVICEAFDEDMNPITVEGFGLFARAICHETDHLDGILYPDVAEEPARDVTMEEVE"
     gene            complement(9990..12215)
                     /gene="priA"
                     /locus_tag="PLC71_01660"
     CDS             complement(9990..12215)
                     /gene="priA"
                     /locus_tag="PLC71_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRM01384.1"
                     /translation="MEKLYADIIINISHEALDKVFAYKVPFSMIKDIRVGQQVVVPFG
                     RSNKEQTGYVVNLSRTVDYDESKVKEILRIETDHVGVESNMIELAAWIRENYGSTMIQ
                     ALKTVLPVQEKVARKARKYIELCIEKVHGPAMLDEYFSKHYVAKARLLAALLDHGKIS
                     WEMASKDLKISKSTVDSMEREGILHVVTEYYYRNPGEFSIAKAEAHVLNEQQQELIDE
                     FREDFLREDHKTYLLHGITGSGKTEVYLAAIEEVIKQGKQAIVLIPEIALTYQTVTRF
                     TKRFGERVSILNSRLSKGERYDQWLRAQRGDVDVIIGPRSALFVPFPNLGLIVIDEEH
                     EGSYKSEQTPKYHAREVAIKKAQMEGASVILGSATPSVESYKHALDGTYRLWELTKRA
                     KEAVLPQVYIEDLREELKAGNRSMFSRRLKELIKDRLNKGEQIMLFLNRRGYAGFVSC
                     RSCGHVMECPHCDISMTYHRDGRLRCHYCGYEQPMLKVCPECGSPYIGTFGLGTQKVE
                     AALYKEFPQAKVLRMDMDTTKRKNSHEQILSAFSDGEADILVGTQMIVKGHDFANVTL
                     VGVLAADLSLHANDYRAGERTFQLLTQAAGRAGRGDKPGEVVIQTYSPEHYSIQTAAK
                     QDYHAFYKEEISYRSLMRYPPEWQMLVVFASGEDKAWLDKVMDAMANVVKHMDLMVIG
                     PSEAGFGKINDQYRKVIYIKNKDYETLVKAKNRLEQWIDLNKIRKNLLVNFDFNPMNS
                     Y"
     gene            complement(12219..13571)
                     /gene="glmM"
                     /locus_tag="PLC71_01665"
     CDS             complement(12219..13571)
                     /gene="glmM"
                     /locus_tag="PLC71_01665"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRM01385.1"
                     /translation="MGKYFGTDGFRGEANINLTVEHAYKVGRYIGWYFNQKSSSRAKI
                     VIGKDTRRSSYMFENALVAGLTASGADAYMLYVTTTPSVAYVVRTEQFDCGIMISASH
                     NPYYDNGIKLINAKGHKMEAEVENEIEAYIDGEIHEIPFATKKEIGRAKDYAAGRNHY
                     IGYLISTATRSFKGVKVGLDCSNGSSFAIAENVFKALGAETHVINNEPDGTNINTNCG
                     STHIEILQGYVKENHLDVGFAYDGDADRCIAVDENGQVVDGDLILYVCGQYLKKHGDL
                     NNNTVVTTVMSNLGLYKAFDAAGIAYEKTAVGDKYVNENMVKNGHALGGEQSGHIIFS
                     KFATTGDGILTSLKIMEAMIEQKQSLAQLAEPVEIFPQILENVRVYDKAVAQADEDVQ
                     KAVKEVEAELGDEGRILVRESGTEPLVRVMVEARSHEICREKVDKVVKVLREKGHVIK
                     "
     gene            complement(13669..14505)
                     /locus_tag="PLC71_01670"
     CDS             complement(13669..14505)
                     /locus_tag="PLC71_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HRM01386.1"
                     /translation="MNMWTRSELKERGKAALHRNYWIVVLATLVVTALTGGTGNAAGN
                     GSLQEGSNIAAGNGDAASNLIQTLFSFFGVAGDMIYKNLIGPMYNGMYVSFILILIIT
                     AFSVGLLFKIFVGNLFEVGGCRFYEENSEHKTRIALIVDGFLNGAYLRNVATIFCRDL
                     YTVLWTLCLIIPGIVKSYEYKMIPYILAENPRISRKRAFEISKNMMDGEKWNAFVLDL
                     SFIGWNLLSTITFGIVGVLYVNPYVNTTWAEFYKVMRENALETGNATREELIGLRKEA
                     DI"
     gene            complement(14633..>15083)
                     /locus_tag="PLC71_01675"
     CDS             complement(14633..>15083)
                     /locus_tag="PLC71_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRM01387.1"
                     /translation="RFGRLAVPVINDEDYKFEIGKGKVLREGTDVAIIANGLCVAESL
                     DAAEKLAAEGINAQVINMATVKPLDTELVLEAAKATGKVVTVEEHSVIGGLGSAVCDV
                     LSEQLPTPVLKIGVNDVFGHSGPAVELIKEFGLDGDSIAAKVKEFVK"
     assembly_gap    15084..15093
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15094..>15421)
                     /locus_tag="PLC71_01680"
     CDS             complement(<15094..>15421)
                     /locus_tag="PLC71_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HRM01388.1"
                     /translation="GIAESNMIGVAAGLATTGKVPFASSFAMFAAGRAFEQVRNSVGY
                     PHLNVKIGATHGGISVGEDGATHQCCEDIALMRTIPGMTVIVPSDDIEAKAAVKAAAA
                     MEGPVYM"
BASE COUNT         3900 a         3724 c         2339 g         5438 t
ORIGIN      
        1 gcatcaaaaa aggaagagta gtattttctc ttcctttttt attctctgtt atgttgtctg
       61 tccttccagt aattttactg gaagatccat gcagctttct gctgtttttc cttcgatcag
      121 atcgatcagc ttatccaccg ctacctttgc aatctgttta actggctgct ggatcgttgt
      181 cagttctgga acaaatccta aaatctgctt tgttccatct actccgatga tctttagatc
      241 tttcggaata ttgattcctt tttgccttgc gacatgaagt gcgatcactg caagcgaatc
      301 atctcctgca aaaataccgt caatctcagg gtgtgcattt aacatatcat aaataagttt
      361 ttgcttctcc acattctcca tgatgaacgg tacttctaaa atcatctgag gaagccctac
      421 tttctccatc tcttgaatat aagcctgact tcgattgtta cctggcatct ttaatttact
      481 gtttcctcgg atacataaaa tgtgctgaca tcctttatcg atcaaatgtc tagttgccat
      541 ctgccctgct gcatagttat cacatgaaac gactggaata tgttctccaa gataacggtc
      601 aattcctacg nnnnnnnnnn tgtctctacg actgcgttat gtgttccaac gatcattcca
      661 tctacctgat tgcgtcttag catatcaata taggctttct ctgtatccat ctgatttttg
      721 ctgttacaca gaagcatctt ataatttttc tgtgataaat aatattctat ctgttctaca
      781 acttctccat gaaatggtga tgttacagta ggaacgataa ccccgacaaa atacgttctg
      841 ttatttaaca acgctctggc aatctcattt ggataataat tgatctcttc catagcatca
      901 tatacttttt ttcttgtttc ttcgctgatg tatcctcggt tatttaatac cctggatact
      961 gttgtgggag aaactcccgc tacttttgcc acatctttta ttccaggttt cattccatac
     1021 cttccatttc taacttattc ttaaagtatc ccatcttctc gaagcagctt tcctacttct
     1081 acatattctt ctctgcttcc ctcaagatat ttgatctcgt cctctgtcaa tgtacgtttg
     1141 actcttgctg gacttcccat aactaaagat ctatctggga tcactgtatt ttgtgttaca
     1201 agacttcctg ccccgatcaa gcagtgatta ccgatctttg ctccattcat aacaacagat
     1261 cccatcccga tcatggaatt atctccgatc tggcatccat ggatcacgct gttatgtcca
     1321 actgtcactc catcgccgat ttttacacta ttcccttcat ctacatggat cgtacaattt
     1381 tcctgtatat tactctgatt tccgatcaca atggactctt ctccttcaca gcgcattgct
     1441 gtaaagaaca gtaccgtaga ttcttctcct atagtaatat tcccaactaa aacagtttct
     1501 tttgctactt ttgctgaatc agcgatcttt gcctgtttgt aattcatgtt ctatctcccg
     1561 atattcttct tattgctctt ctgagagtag ttcattcttc cttctttcca acccttcttg
     1621 tctgtcttat cccagtctag gttgtctccg aagatatatc ttgtcatcca taataagctg
     1681 accgttggaa ccactaccca taatactctg tattcaagtt ccattaaaaa tggtaaaaat
     1741 gccagtcctg ctgcgtggat cagtaagatc actgcccatg cgatgatcag ccagtaaccg
     1801 atcttaaatg cttctggcat atctcttccc atttcttttc caaaataaaa acgttctgct
     1861 gataaaatgg cggcagctaa tactgtcatg atcacacaca ttttatagaa tacgttacca
     1921 tccatatatt gtcctaaaaa cattccgatc aatgatacga taaacatcag gatcagaaca
     1981 gcccagagaa ccttttcaag cttacttgtt actttgtttg ttgttacttt ttctttcttc
     2041 atcttgtata acctcaatct ctactttcta tcagaagcag ttttccttca ctaaatgaaa
     2101 tgattccttc ttctgcttct aattcattac tgatcgcgag cgattgctca aacactaatg
     2161 tatggtcttt taatggatat ttaaatcctg ttagtgtgac acctcgtgcc tcatatattg
     2221 gtatcaagga tacatattta ccaaactggt ctttctttga tattgtatct tcatgattta
     2281 aaaccctgat acgattgttt gcatcaacaa gttctgcctt cacacctttg ctgtgtgcgt
     2341 aaaacaactg tccgaaattg cccataacat gatcgagtct tgttccagtt ccacctatca
     2401 taaggatttc atctgcacct gtctcaattg ctttttgtat tgcaagtcct gtatcagtat
     2461 cgtctttttc acatggtacg ataatcttct tgtctgttga aaattcttgc attatatcct
     2521 tactgcaact gtcaaaatca cctaaaataa tatctggttt tatattcagt ctcttagcat
     2581 aaagcagtcc tttatcagct gtaataacct gatctgggtt ccatttactg atatagtctt
     2641 tcgcgaaatt ttcttcgact gttccgcctg ttaaaatcaa tactcgcatt accattttct
     2701 cttttctttt agttctgtat aaatatttac ataattatca taacggatct ttgagatctt
     2761 tccttcttcc aatgcctttt tcactccaca gtctggttca tgtgtatgac tgcatccctg
     2821 gaatcggcat tcatcttcat atggtgcgaa ttcctgaaaa tagtctttga tctcatcttc
     2881 ttcaaacata tgaatgaata aagaactaaa ccctggggtg tctaggacat acgtatcttt
     2941 ctcgatacgt atcagctctg aatgtcttgt tgtatgacgc cctcgcttga tcttctcact
     3001 gacttcccct gtttccatct gtgcctctgg ctgcagaagg ttcatcaaag atgattttcc
     3061 gacccccgat ggtccagcaa gaaccgtcgt cttaccgtgg atcagttccc tgatctcatc
     3121 aattccttct tcttccttaa cgctgataaa acgaacgata tgtccacagt tttcgtagac
     3181 tttctctaat aatttcagtt ctttttcttt tgcaagatct accttgttaa agcagacgat
     3241 cacaggaaca tcctgtttct ccatcatcat cagaaaacga tctaacagat tataatttgg
     3301 ctttggattc tttgctgcaa atatgatcag tgcctgatct acatttgaca cagctggacg
     3361 gatcagctcg ttatgtctct catagatctt tgtaatattt ccttcctgct ctgtctcatc
     3421 taaaatatcg atttctacat tgtctccgac taacggtttc atcttcttgt ttcggaagat
     3481 tccctttgcc ttacattcat ataatttatc ttctgtctgt acgtaataaa atcctgcgat
     3541 tccttttatg atctttcctt gcatgaaata tctcctgcac ctgcttttcc tttattcttc
     3601 atctgttgtt gttgattctt catccgctgt tgtcgctgtc tgacgaggtc caagactgac
     3661 atagatccca acggctgttc ccttcttaac cttgcttcca gaactgatcc tctgacgaat
     3721 gacaagtcct ttttccacac tgtcactata ttctgttaaa acacttccca atgataatcc
     3781 gaacctttgc agttcctgac ttgcttcaga ttccgtgtaa taacgaagat ttggaacgct
     3841 gaccgtctga tcttccttct tgcctttgct gactgtgatc acaacagttg tatttttcga
     3901 aactttactt ccatgtgatg gagtctgaga gataacttct cctttcgcaa catcatcact
     3961 gtaagcttct tttattgaga ctttgagtcc tgctgctttt aattcactct gtgcaccaga
     4021 cttcgtctta cttcgcacat caggtactga aactttatct tcgatcttct gtcctttgct
     4081 cacaactaac ttgatcgttt ttccttcttc taattccgta tcgctttctg gatcttgctg
     4141 catgaccagt ccttttgcat acgtatcgga atattcttct gtcacgttga tcttgaactt
     4201 gttatcaatc gcctctctag ctgcttctaa agacaatccg atataattat caactgtgta
     4261 tttttctgta gtattgtgat gtttggcttc tgttgttgct gcgatcgtac tttgtgttgt
     4321 tgattttcct gatttgaata atccagaact tttgacaaca aatacgacaa tagaaatcaa
     4381 aatgatcgca cctaccactg ctgcaagaac aatgacaagt ttctcgatct ttgagttcat
     4441 tcctgtttct tcttcctcat catcctcttc cagatatgca ttgctatctg aatcttccac
     4501 aggctgtgta tcatcatttg tctggattct ctgtgtcagt tcgttctgat cgatcatgat
     4561 cgttggagaa tcatcgatcg ttggaacaat tcccacatat tcaccagaag tatcttcaaa
     4621 cacgagtttc agatctgcga tcaattcatc tgctgtctga tagcggtctt ctggcttctt
     4681 cattgtacat tttaaaatga tcttctctaa actgtctggt attccatctc tgatctgact
     4741 tggcggtgtg atctcatttt gcagatgcat caatgcgatc gttacaccat tttcatgatc
     4801 aaatggcaca tgtcctgtta ccatctcata catcgtgata cctaaagaat aaatatcact
     4861 tttttcatca cagaatcttc cttttgtttg ttctggagaa atgtaatgca cagatcccat
     4921 cgctgttgct gttgatgtaa cggtattgct gcttgttgcc ttcgcaattc caaagtctgt
     4981 gacctttaca tttccaatct ctgaaactaa aatattctgt ggtttaatat ctctatgaat
     5041 aatatgattc ttatgggcat gtgagatcgc tgacgcgatc tgcagggaaa tctctaccgt
     5101 ctccttcgga ggtaaatacc cttttcttcg gatatattct ttcagagtca tcccttctgc
     5161 aagctccata acaatataat tgatcccatc ttcaattcca acatcgtata cattcacgat
     5221 attaggatga gacagccttg caactgcctg tgcctctgtc tcgaatttct ttaaaaattt
     5281 ctcatcttct acgtattcac tttttaaaac cttcactgcg acgttccggt ttaatttatg
     5341 atctttcgcc atataaacgt ctgccattcc accgcttcca acgcgcttta gcacttcata
     5401 acggcctgac agcatcgtgc caatctctaa catctatcat tcacctcatc tctttaaatc
     5461 cacaattacc aatgaaatat tgtcttttcc tccgtactcg ttcgcacggg tgatcatctg
     5521 gttgacctta ttggcaatct gactgtgctc aagaagaagt tccttcaatt cttcgtcgga
     5581 tatcatattg gaaagtccat cactgcacaa aagcaaatat ccatctttgg catcaaactc
     5641 aaagaaatct ggtgtcgcca ttacggtacc tagtgctctt gtgatgacat tcttctttgg
     5701 atggaatctt ccttcctctc tttccatgat tcctgcatga atcagttctt ccacataaga
     5761 atgatccact gtaatctgtg ttaattcttg atttccataa tataatctac tatcaccaat
     5821 attcataacc gttactttgc catcagagat ggttgctgca acgaatgttg ttcccattcc
     5881 cctatattct tcattactct ggctttctgc gatcatttcc tgattcacag acgcaatgga
     5941 tgatttcatt aattcgatcg gatcggtctg tttcgattcc tcgatcagct gtacaaatcg
     6001 ttccactgca aaagaggagg catgcccacc ggcacggtgt cctcccattc catctgccac
     6061 gatataaaga ttttttaagt ttccaacagg ctggtcatga aagaaaatcc tatcttgatt
     6121 ctctgtcctt accttgccta tatgtgaagc ccctacggat cgcatatcgt tctcctttac
     6181 aatcttgact gcagtttttt tcttctaagc tgtccacagg ccgcattgat atctgcgccc
     6241 atttctcttc ttatggtaac atttacacga tttttttcaa gtatcttttt aaattccata
     6301 acggatttcg gcatagattg cttaaaatca cgctctttga tcggattaac tggaatcagg
     6361 tttacatgac atggaaaacc tttcaatctg cctgctaatt cttctgcatg aactggctgg
     6421 tcattaaccc ctgccacaag gctgtattca aatgtcatcc tacggtttgt ctctttaaaa
     6481 taataatgac atgcatctag cagctcatcg attgaatatt tcattgcgat cggcatcagt
     6541 cctcttctca tctcatcatt tggtgagtga agagaaatgg ctaatgtgat ctgtaaatgt
     6601 ttctgtgcaa gttctttgat ctttggcacg atcccacacg ttgagatcgt aatatttctc
     6661 tgactgatat ttaatccatc ctctgatgtg atcagttcta aaaatctcaa tacgttatca
     6721 taattatcca tcggttcccc tgttcccatc atgacaacat tggatactct ttcttctgta
     6781 tctctttgaa tataataaat ctgtgaaagc atttcagatg gcgtcagatt acgatctaat
     6841 cctccaagcg tagatgcaca aaacttacat cccatacggc accctgcctg agatgaaata
     6901 cagacagaat ttccatgttt atacttcatc aaaacacttt cgataatatt cccatcatat
     6961 aactcaaata agtattttct tgtaccatca atttttgaaa taaagcagtc tacaggtttt
     7021 acaccataaa tctcctgtgt ctgttcgatc ttttctttta atttttttgg tacattattc
     7081 atctcatcaa gagacagagc aagatgtttg tgaaaccatt cataaatctg ttttgcacgg
     7141 aatttcttct caccaatgga ttccatatat tcctgcatct cctgaagtgt cattgatttt
     7201 aaatccataa ctttattttc tccttaattt accaataaag aatccatcac atggatgtat
     7261 tcctggcaac aactgaataa atccttcttg ggcagtttca atctttagat cctttggcag
     7321 ttcttttgta atatcaactg cttcaaaatc aaagtgatca agaatccatc ggaaattttc
     7381 ttcattctct gctggattga tcgtgcaggt actatagatc aatgtacctc catgcttgac
     7441 atattgcact gcatttgcaa gaatctctcg ctgcaacttc acaagatcct tcatgccttc
     7501 ttctgaaatg tggtatttaa tatcattttt ctttcccatg atcccaagtc ctgaacatgg
     7561 tacatctgcg atcacaatat ccgcagcctc aatatgttct tcatccaaca ctctcgcatc
     7621 aaatacttcg gattccatat tcgtaaatcc aacacgatca atgttatctt cgatcagatc
     7681 tgtcttgtat tccgttagat cccttgataa cactcgcccg cttcccttca gctgatctgc
     7741 aacatataag gacttaccac ctggtgcact gcaaagatcc atcacaaact gctctttctg
     7801 gacactggca agtttggcac acaacatgga actttcatcc tgtacataac aacttccttc
     7861 acgaaatgct ttcaaacgat acaaaaaatc ataatctgag atctgcactg cattttcgat
     7921 caaacttcca tcttctactg taacgccttc tgcttccagt tcttttctga cagcttcttt
     7981 cccacccttg gaaatcagac aacataatgt tgtttttctc tcttccagat aagaagctat
     8041 gatcttctct actgtttcgt tatcatatgt cttctgaaag aattttatga tccattctgg
     8101 aatagaataa gcaactgata aatatgtctc aagattcttc tctcttgatg gatattggat
     8161 ctcatctttc ttccttgaaa tattacgaag tacaccgttc acaaaacctg ataatcggga
     8221 aaatcctcgt ttctttgcaa gtttcactgc ctcattacat gcagcagaat ctggaacctg
     8281 atccatataa agaatctgat agattcccat acgaagcaat gcacggatca gtggtttaca
     8341 ttttctgatc ttcgtctttg agaactgatc tgcaacatag tcgatcgtga tctgacgctc
     8401 taaagttcct tccaccagtc ttgtaaaaaa tgcacggtct cttttttcag aaaactggta
     8461 tttttctaat acatttgaaa ttgcgatgtg tgataattct tcttttttat ctactttgat
     8521 cagtacatcc aatgcacatt ctctcggatt ttgcatcgta tcattctcct aattttgttc
     8581 cggcttctac ttgatatcca agtaaaaatg cctgtacctg catacgtttc tttcctgcca
     8641 gctgaatctc atgaagtgca agatcattgc ttcctgttgc tacgatgatc tcttttttgt
     8701 tgacttctac gatcgttcct ggttcttctt tactctgtgc atcaagcaca tctgcttccc
     8761 agactttcat tgtctttcca tctaaggatg tataagcact tggccatgga ttcattccac
     8821 ggatcagaca ttcgatttct cttgctgatt ttgtgaaatc aatctgtccc atatctttac
     8881 ttagcatttt tgcataacag ctttcttcgt cattttgttt ttctctgacg gctgttccat
     8941 cttctaattt ctttaatgtt tctaataata aatttgctcc taataccatc aatttatcat
     9001 gcagagaacc agctgtttct ttcttatcta aaacaacttc tgctttatcg atcatatctc
     9061 cggtatctaa tcctttttcc atatacatgg ttgtgattcc tgtcttttct tctccatcaa
     9121 gaactgccca ctggattggt gctgctcctc tgtattttgg aaggagtgat ccatgaacat
     9181 tgatacatcc atactttgga atatttaaga tactttctgg taagatctgt ccataagcaa
     9241 caacaacgat cacatctggg ttgaggtttt ttaactcttc aatgaattcc tcatctctgg
     9301 ctctctgtgg ctgatatact gggatatcat attccactgc tttttctttg actggaggaa
     9361 actgcatctt ctttcctcta cctctttgtt tatccggctg tgtcacaaca gcaagcacct
     9421 catggtgatc gatcagtgcc tgcagtgttg gtactgcgaa ctccggtgtt cccataaata
     9481 ctgctttcat cttattctac ctcttccatc gtgacgtctc ttgctggttc ttctgctaca
     9541 tctggatata aaattccatc caaatgatct gtttcatgac agattgctct tgcaaataaa
     9601 ccaaatcctt ctactgtgat cggattcata tcttcatcaa atgcttcaca gatcacatga
     9661 tctggacgtt tcacaactgc tacttttcct ggaaggctta aacatccttc ttctcctacc
     9721 tgctctcctt ctttcagtgt gatcactgga ttgacaagag ttacacattc tccctcttct
     9781 ccagtatcaa tgactacgat tctttttaag attcccacct gaggtgctgc aagaccaaca
     9841 cccattgctt catacattgt atcatacata tcttctacta atgtatgcat tcttggtgtc
     9901 atttctttta cttctttaca tttttttctt aaaacatcgt ctcctaatac tcgaatctgt
     9961 cgaattgcca taattgttct ccctttcttt taataactgt tcattgggtt aaaatcaaaa
    10021 ttgaccagta aatttttcct gatcttattt aaatcgatcc actgttccag acggttcttt
    10081 gctttcacca gtgtctcgta atctttattc ttaatatata tcactttgcg gtattgatca
    10141 ttgatcttgc caaatcctgc ctcgctcggc ccgatcacca tcagatccat atgttttacc
    10201 acatttgcca tcgcatccat tactttatca agccatgctt tatcttctcc tgatgcaaat
    10261 acgaccaaca tctgccattc tggtggatat ctcatcaagc ttcggtagct gatctcttct
    10321 ttgtaaaatg cgtgataatc ctgtttggct gcagtctgga tactgtaatg ttctggggaa
    10381 tatgtctgga tcactacttc tcctggtttg tcgcctcttc ctgctcttcc cgcagcctgt
    10441 gtcaataact ggaatgttct ttctcctgcc cggtaatcat tggcatgcag ggaaagatct
    10501 gctgccaaaa ctcctaccaa tgttacattc gcaaaatcat gtcctttgac gatcatctgt
    10561 gttcccacta aaatatctgc ttctccatca gaaaatgccg ataagatctg ttcatgacta
    10621 ttctttctct tcgttgtatc catatccata cgaagcactt ttgcttgtgg aaattctttg
    10681 tataatgcag cttccacttt ctgggttcca agaccgaatg ttccaatata cgggcttcca
    10741 cactctggac aaaccttcag catcggctgt tcatatccgc aataatggca gcgcagtctt
    10801 ccatctctgt gatatgtcat agaaatatca caatgaggac attccatgac atgaccacag
    10861 cttctgcatg aaacgaatcc cgcataacca cgccggttta agaacaacat gatctgttcg
    10921 cctttattca aacgatcctt tatcagttct ttcagtcttc ggctgaacat gctgcggttt
    10981 cccgctttta attcttctct tagatcttca atatagactt gcggtaacac tgcttctttg
    11041 gctcgttttg tcaactccca cagccgataa gtcccatcca gtgcatgttt ataactctca
    11101 actgatggtg tcgccgaacc taagatcacc gatgcacctt ccatctgtgc tttcttgatc
    11161 gcaacttctc ttgcatggta tttgggagtt tgttcactct tgtaactgcc ttcatgttcc
    11221 tcatcgatca caatcagccc aagattcgga aatggaacaa acaatgcaga gcgtggtcca
    11281 atgatcacat ccacgtcccc cctctgtgca cgaagccact gatcgtatct ctcacctttg
    11341 gataatcgtg aattcagaat tgatacacgc tctccaaatc gtttggtaaa ccttgtaacc
    11401 gtctgataag tcagtgcgat ctctggaatc aggacgattg cctgttttcc ttgtttgatc
    11461 acttcttcga tcgctgcgag ataaacttct gtcttcccac ttcctgtgat tccatgtaaa
    11521 agataggttt tgtggtcttc tcttaaaaaa tcttcccgaa attcatcgat cagttcctgt
    11581 tgctgttcat ttaacacatg cgcttctgct tttgctatac tgaactctcc aggattccgg
    11641 taataatatt ctgtcacaac atgaagaatt ccttctcttt ccatgctgtc tactgtactt
    11701 tttgaaatct ttagatcttt cgatgccatt tcccatgaga tcttcccatg gtctaataaa
    11761 gctgcaagaa gtcttgcttt cgccacataa tgcttactga aatattcatc aagcatcgct
    11821 ggtccatgca ctttctcaat gcatagctca atgtattttc tcgcttttcg tgcaactttt
    11881 tcctgcactg gaagtacggt ttttaatgcc tggatcattg ttgatccata attctctctg
    11941 atccacgcag ctaactcgat catatttgac tccacaccaa catgatccgt ctcaatcctt
    12001 aagatttctt tgactttact ctcatcatag tcaactgtcc gtgaaaggtt gactacatat
    12061 cccgtctgtt ccttattaga acgtccaaat gggacaacga cttgctgtcc cacccgaata
    12121 tccttgatca tggaaaaagg caccttatag gcaaacacct tatccagtgc ctcatgtgaa
    12181 atattaataa ttatatccgc atataatttt tccatagatt atttgattac gtgacctttc
    12241 tctcttaaaa ctttgaccac tttatctact ttctcacggc agatctcatg gcttcttgcc
    12301 tcaaccataa cacgtactaa tggttctgtt ccactctcac gtacaagaat tcttccttca
    12361 tctccaagtt ctgcttctac ttccttaact gctttctgta cgtcttcatc tgcctgtgca
    12421 actgctttgt catatacacg gacattttca aggatctgtg ggaagatctc aacgggttct
    12481 gctaactgtg caaggctttg tttctgctcg atcattgctt ccatgatctt taaggatgtt
    12541 aaaataccat ctcctgttgt tgcaaacttg ctgaaaatga tatgtccaga ctgttctcca
    12601 cctaatgcgt gaccattctt caccatattc tcattaacat atttatctcc aacagcagtc
    12661 ttctcataag caattcctgc tgcatcaaac gctttatata atccaagatt ggacattaca
    12721 gttgtaacaa ctgtattatt gttcaagtct ccgtgtttct tcagatactg tccacataca
    12781 taaaggatca gatctccatc gacaacctgt ccattctcat cgactgcgat acaacggtct
    12841 gcatctccat catatgcaaa tccaacatct aagtggtttt cttttacata tccctgtaag
    12901 atctcgatat gtgtagatcc acagtttgta ttgatatttg ttccatctgg ttcattattg
    12961 atcacatgtg tctctgcacc aagtgcttta aatacattct ctgcgatcgc aaatgagctt
    13021 ccgttagagc agtctaatcc gactttgaca cctttaaatg atcttgttgc tgtagagatc
    13081 agatatccga tataatgatt tcttccggct gcatagtctt ttgctcgtcc aatctctttc
    13141 tttgttgcaa atgggatttc atggatctcc ccatcaatat atgcttcgat ctcattttca
    13201 acttctgctt ccatcttatg ccctttggca ttgatcaatt tgattccatt atcatagtat
    13261 ggattgtggc ttgctgagat catgatcccg cagtcaaact gttctgttct tacgacataa
    13321 gccacacttg gtgtcgttgt tacataaagc atatatgcat ctgctccaga tgctgtcaat
    13381 cctgctacta aagcattctc aaacatatag ctgcttcttc ttgtatcctt accgatgaca
    13441 atctttgctc tgctgcttga cttctggtta aaataccatc caatataacg tccgacttta
    13501 tatgcatgtt ctacagttaa atttatattt gcttctccac ggaacccgtc tgttccaaaa
    13561 tattttccca tcttaatact ccttgcatcg tatcgttttt gtttttacca cctgacatta
    13621 taccatgatt acgatcgtct atcaaatata gaatcaatga agtttttctt aaatatctgc
    13681 ttcttttcta agcccgatca gttcttctct tgttgcattt cctgtttcca atgcgttttc
    13741 cctcatgact ttataaaatt ctgcccatgt tgtattaaca tacggattca catataaaac
    13801 tcctacaatc ccaaatgtga tcgttgataa cagattccat ccgataaatg acagatcaag
    13861 tacgaatgca ttccattttt ctccatccat catattttta ctgatctcaa acgctctctt
    13921 ccttgatatc cgagggtttt ccgcaagaat gtaagggatc attttatatt cataactttt
    13981 cacgattcct ggtataatta aacacaacgt ccacagcact gtataaagat ccctgcaaaa
    14041 gatcgtagca acatttctta agtatgcacc attgaggaat ccatcaacga tcaatgcgat
    14101 tcttgtctta tgttcgctgt tctcctcata aaaacgacag cctccaactt caaacaggtt
    14161 tcctacaaaa atcttaaaca acaaacctac actaaaggct gttatgatca agatcagaat
    14221 aaatgacaca tacattccat tatacatcgg acctatcaga tttttataga tcatatcacc
    14281 tgcaactcca aaaaacgaaa ataatgtttg tatcaaattt gatgccgcat caccatttcc
    14341 tgctgctata ttacttcctt cctgcaggga accattccct gctgcattac cagttcctcc
    14401 cgttaatgct gtcactacaa gtgttgcaag cacaacgatc caataattgc gatgcagtgc
    14461 tgccttccct cgttccttca attcacttct tgtccacata ttcattcacc tctccttttt
    14521 gtaaattgtt ttgatcctaa aatcattttc atcataacac aaaagcagta cacctgctac
    14581 aaaaacaagt gtactgcttt ttattcttgg tttattatgc ttaaaagaaa atctatttta
    14641 caaattcttt taccttggca gcgatactgt caccatctaa tccgaattct ttgatcagtt
    14701 ctactgctgg tcctgaatgt ccgaatacgt cattcacacc gatctttaat actggtgttg
    14761 gaagctgttc agataataca tcacatacag cacttcctaa tccaccgatc acagaatgtt
    14821 cttctactgt aacaacttta ccagttgctt tcgctgcttc taatacaagt tctgtatcta
    14881 atggttttac agttgccata ttgatcacct gagcattgat tccttctgct gctaatttct
    14941 cagctgcatc taaggattca gcaacacata atccattagc aatgatcgct acgtctgttc
    15001 cttctcttaa gactttacct tttccgatct cgaatttgta atcttcatcg ttgatcactg
    15061 gtacagccag acgtccgaat ctcnnnnnnn nnncatataa acaggtcctt ccatcgctgc
    15121 tgcagctttt actgctgctt ttgcttcaat atcatcagat ggaacgatca ctgtcattcc
    15181 agggattgta cgcattaatg caatatcttc acaacactga tgtgtagctc cgtcttcacc
    15241 tacggaaata ccaccatgag tagctccgat cttaacattt aaatgtggat atccaacaga
    15301 gtttcttacc tgctcaaatg cacgtcctgc tgcgaacatt gcgaaagaac ttgcaaatgg
    15361 aacttttcct gttgttgcaa gacctgctgc aacaccgatc atattagact cagcaatacc
    15421 a
//
LOCUS       DAOMUE010000016        15341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_800_length_15341_cov_2.997907, whole genome shotgun sequence.
ACCESSION   DAOMUE010000016 DAOMUE010000000
VERSION     DAOMUE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 15341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15341
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_800_length_15341_cov_2.997907"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1815)
                     /locus_tag="PLC71_01685"
     CDS             complement(70..1815)
                     /locus_tag="PLC71_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRM01389.1"
                     /translation="MTKEIMNAVNTQIFGVWFLIGAALVFWMQAGFAMVEAGFTRAKN
                     AGNIIMKNLMDFCIGTCVFILIGFSLLLGEDMIGLIGKPGFDIFTSYANFDWSSFVFN
                     LVFCATTATIVSGAMAERTKFLSYCIYSAVISALIYPIEAHWIWGGGWLSQMGFHDFA
                     GSCAIHMVGGLSALIGAKFLGPRIGKFTKDKDGNITKVNAFPGHNIPLGALGVFILWL
                     GWYGFNGAAATSVEQLGSIFVTTTISPAVATVVCMVFTWVKYGKPDVSMSLNASLAGL
                     VAITAPCDVTDAFGAIVIGAVSGLLVCFGVWLLDYKLRIDDPVGAVAVHFMNGLWGTL
                     ATGLFATKTAPGSELNGLFYGGGFGLLKIQIIGIVCVCAWTAVTISLTFTIIKATIGL
                     RVTEEEEIIGLDGPEHGLTSAYAGFSMMDISNTMIMEENANTVLGEEDYERASKVQKA
                     AAIQVSKAPDLVPTGMYKVVIIAKLTRYDKLRKAMNDVGVTGMTVTQVMGCGIQKGAG
                     ERYRGAEVDATLLPKVKVEVIVGNIPVENIIEAAKKALYTGHIGDGKIFVYNVDKVIK
                     IRTGEENLAALADVE"
     gene            complement(2063..2776)
                     /locus_tag="PLC71_01690"
     CDS             complement(2063..2776)
                     /locus_tag="PLC71_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM01390.1"
                     /translation="MTEIKFNVLIAEDEENIGNFISTILKTNGYKPIIATTGNQAIQC
                     AESYCPDVILLDLGLPDMDGIDVISEIRKWAVTPIIVISARNRESEKVKALDAGADDY
                     ITKPFGNQELLARIRTALRHRRQPMGAETKTPMYHAGQLKIDFDKRRVFLGEEEIRLT
                     QIEYKLVSLLAENAGKVITYESIIKDIWGPHADTNNRILRVNMANIRRKIEKNPAVPK
                     YVFTEVGVGYRMLENELNS"
     gene            complement(2773..3924)
                     /locus_tag="PLC71_01695"
     CDS             complement(2773..3924)
                     /locus_tag="PLC71_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRM01391.1"
                     /translation="MEQNEKGLMQWEKDFLISITILIIATIIAHIFFYQTAHNINVPM
                     IYTLATFFISRYTKGYGWGIVSSLLSVIFINWRFTFPYFKINFMIDGYPMTFLGILVI
                     AIITNMTTTSLNREKEAAVKREKELAKLNEYNKQINELQIEKEKEKMRSNLLRAISHD
                     LRTPLTGMIGASSTYLEAKDQLDEDAKDQLVMGIKEDANWLLNMVENLLSITRIQSNG
                     SDTQPHVKKVPEPLEEVVAEAIQRYQKRYPDSELKVKIPEDFLMVPMDPTLIEQVIMN
                     LLENAWVHSGSDAPIEFDIKEEGNKVVFYIRDHGNGIAPERLDQLFDGCAGALDHESR
                     KGMGIGLSICKSIVMAHQGDVYARNYEDGAEFSFSLPMDEKELTEEKEL"
     gene            complement(3927..4349)
                     /locus_tag="PLC71_01700"
     CDS             complement(3927..4349)
                     /locus_tag="PLC71_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HRM01392.1"
                     /translation="MTKDILINISGLQMDVDPNDPIEMMTTGAYYLRNGKHYILYDEL
                     SEDNEIVKNVIKIGPKSVELTRKGGQSSHMVFEEGKENLSYYDTPFGSLLMGVNTSNI
                     DWIEEEEQMHLKVDYDLSMNSDHVSKCKIDVNIRPNGE"
     gene            complement(4364..5167)
                     /gene="murI"
                     /locus_tag="PLC71_01705"
     CDS             complement(4364..5167)
                     /gene="murI"
                     /locus_tag="PLC71_01705"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRM01393.1"
                     /translation="MNAPIGVFDSGVGGLTVAREIMRQLPEESMIYFGDTARVPYGTK
                     SKDTIVRYSRQIVNFLLSKGVKAVVIACNTASALALADLQELYNVPIIGMVQPGAIAA
                     MNATKNKNIGIIGTNATIKSGQYGQYLRKLDPSVTVVTKACPLFVPLVEEGLIDDRIT
                     EDMVSRYLREFKQYDIDSLILGCTHYPLLINPIQRFVGDKVTLVNPAYEVAKTLKQML
                     AQRDMAASEDHTAKYEYYVSDMADQFLSFADKVLPCRVDQVQPVDIENY"
     gene            complement(5180..6241)
                     /locus_tag="PLC71_01710"
     CDS             complement(5180..6241)
                     /locus_tag="PLC71_01710"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HRM01394.1"
                     /translation="MKKNVVVIFGGDSSEHDVSCLSATTVIKNMDTEKYNVILVGITK
                     EGRWLLVDGVKDIEDGSWREGEVKAFISPDTTTRSLVILAEGTYKLQKVDVIFPVLHG
                     MNGEDGTVQGLFELSKIPYVGCGVLASAVSMDKVYTKIIVDHIGIDQAKFVHVRESDF
                     EHLEEAMDRVEKEIPYPIFVKPSCAGSSKGVSKAENRKELEAALYEAVKHDRNILCEE
                     TIVGREVECAVLGDRTQVKSTCVGEILAAEEAAFYDFDAKYNNAESKTVVDPEMPEES
                     KRKIKEDAEAIFRAVDGFGLSRVDFFLEESGRVVFNEINTLPGFTSISMYPMLWKACG
                     LDIPELLDTLIDQAMTRYR"
     gene            complement(6293..7099)
                     /locus_tag="PLC71_01715"
     CDS             complement(6293..7099)
                     /locus_tag="PLC71_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRM01395.1"
                     /translation="MILLWILVLIIVYIKMIDPGKKRKHPVLNVKYYAHRGFHGEEGI
                     PENSMAAFKKAKRSGYGIELDVQLTKDGVMVVHHDYDLKRTCGVNKKITDLTYRELCR
                     YRLMGTRERIPRFVEVLREVDGKVPLLVELKMETCNRKLCKKVAKALDQYKGLYCMEC
                     FHPYALYWFKKNRPEVIRGQLSEQFLKEKEEGTFTRKIGYFIVKNLLTNFITKPDFIA
                     YHYKYKDCLPLKICRRFYKIPIYGWTFRDKKAYKENEPYFEGFIFEKFVL"
     gene            complement(7096..8442)
                     /locus_tag="PLC71_01720"
     CDS             complement(7096..8442)
                     /locus_tag="PLC71_01720"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRM01396.1"
                     /translation="MKIKMPAQAAKVIQTLEQHGFEAYIVGGCVRDSILGRTPGDWDI
                     TTSASPQEVKEIFDHTVDTGIEHGTVTVLMNHEPYEVTTYRVDGKYEDHRRPNEVHFT
                     KSLREDLLRRDFTINAMAYNDSEGIIDMYDGMTDLKNQTIRCVGEAKKRFDEDALRIL
                     RALRFQAQLGFQIEEETEEAIKNQAKFLKDIIAERIQVELEKLITSAHPEVLVNAYKL
                     GVTKIIFPEFDIMMETPQNNPHHKYNVGIHTIEAMKQIKAEHIYRWTMLLHDVGKPTA
                     RVEGPDKDHFKMHPVIGEEMARKILRRLKFDNQTIKQVTTLVRWHDRRFASIEEVNKK
                     TVRRWVSQLTPELFTRLMEVQKADISAQSDYQRDKKEQVLQETKKLFEEIIEEKNCLS
                     IKELKINGKDLMDMGVPQGKEIGQILSWLLDQVLEDPQLNERETLTRMAEEKRDEK"
     gene            complement(8579..10015)
                     /gene="proS"
                     /locus_tag="PLC71_01725"
     CDS             complement(8579..10015)
                     /gene="proS"
                     /locus_tag="PLC71_01725"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRM01397.1"
                     /translation="MAKDKKLVEAITAMEDDFAQWYTDVVKKAELADYSSVRGCMILR
                     PNGYAIWENIQKVLDAKFKETGVENVAMPMFIPESLLKKEEDHVEGFAPEVAWVTHGG
                     DKELQERLCVRPTSETLFCDFYSKIIQSHRDLPKLYNQWVSVVRWEKTTRPFLRTSEF
                     FWQEGHTAHATEEEAEARTVQMLNMYADFCEQYLAIPVVKGQKTEKEKFAGAHSTYTI
                     EALMHDGKALQSGTSHNFGDGFAKAFDIQYTDKDNKLQYVHQTSWGMSTRIIGAIIMV
                     HGDDSGLVLPPRIAPVQTMIVPIMQKKEGVLDKAEEIRKRLASSYKVKVDDSDKSPGW
                     KFSEQEVRGIPTRIEIGPKDIEKNQAVIVRRDTREKIIVSLDEIETKLGEVLEQMQND
                     MLERAKKHLEEHTVEARNWDEFKAHIEKQDGFVKAMWCGETECEEAIKDETTATTRCM
                     PFEQEHLSDVCVHCGKPAKKMVYFGKAY"
     gene            10148..11482
                     /locus_tag="PLC71_01730"
     CDS             10148..11482
                     /locus_tag="PLC71_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM01398.1"
                     /translation="MANENKMGYLPIKKLILQMSLPPMCSMFLQYSYNLVDSMFVANI
                     NENALAAVSLSFPITTLMISLSIWIGVGINVLIAGYLGEKEQDLADCATTLGLLVALI
                     VGICINILALLIMKPYFSAFTNNPAIFDYAMQYMSVCAFMQVPNMVHIAIQKVFQGTG
                     NMITPMWFQIAGVIFNFVFDPLLIFGIGPFPKMGIRGAALATVLGYALSMVIAFLLLV
                     FTKQKVRIKIKGFHVDSHMLKNLFSYGFPSFVMNALGSFMLTFTNLFLTAYSDTVVAF
                     FGAYFKVQQMIFMTVNGLIQGCLPVMRFNYSAKQPERLKEAFHFGTQAATVMMIGGTC
                     LLLFLPKQILFLFAASESMIKIGVPAMRILSIGLVFCGFSIMIATYQQAIEKIQSSML
                     IHILRQGILLVPLMWLFNHMFGINGIWISFPVTEILVFIVAVLLQTVISKPQ"
     gene            complement(11606..12754)
                     /gene="fucO"
                     /locus_tag="PLC71_01735"
     CDS             complement(11606..12754)
                     /gene="fucO"
                     /locus_tag="PLC71_01735"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HRM01399.1"
                     /translation="MVNRIVLNETSYHGAGAIQEIATEATKRGFKKAFVCSDPDLVKF
                     GVTKKVTDVLDNAGLAYEVYSQIKPNPTIENVQTGVAAFKAAGADYLIAIGGGSSMDT
                     AKAVGIIINNPEFEDVRSLEGVADTKKPCVPIIAVPTTAGTAAEVTINYVITDVEKKR
                     KFVCVDPHDIPVIAVIDPEMMSSMPKGLTAATGMDALTHAIEGYTTKAAWEMTDMFHI
                     KAIELISDSLRGAVENTPEGREGMAMGQYIAGMGFSNVGLGIVHSMAHALGAVYDTPH
                     GVANAILLPTVMEYNADATGDKYKYIAKAMGVEGVEDMTQEEYRKAAVDAVKKLSADV
                     GIPADLKEIVKEEDIQFLAESAYADACAPGNPKDASVEDIIGLYKSLL"
     gene            complement(12890..13438)
                     /locus_tag="PLC71_01740"
     CDS             complement(12890..13438)
                     /locus_tag="PLC71_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HRM01400.1"
                     /translation="MDVSEKMKYKLANAMKELLVHTPVDKITVKQIVDQCDVTRPTFY
                     RHFKDKYDLINWYFDVLAQMSFKQMGISLTLREGLLKKFEFIKGEGQFFAAAFSSESQ
                     NCLMEYDYQCIYQFYCDIIHKQGVDKIPEELEFLLRMYCRGSIAMTVEWATTGMKMSP
                     EQLADQLIDAMPPKLYDLFKDI"
     gene            complement(13571..14587)
                     /locus_tag="PLC71_01745"
     CDS             complement(13571..14587)
                     /locus_tag="PLC71_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRM01401.1"
                     /translation="MAAKRDLYEVLGISKTADEKTIKKAYRKLAKKYHPDMNPGDKTA
                     EQKFKEATDAYNILSDPEKRKLYDQYGHAAFDGTGAESEFYKNYQNYGGAHGGYQEFH
                     FEGGDGDDIFGDIFGSMFGGGRNNKGFHRSSFHQGGFGSSDGFGGGGFGSGSFRQKGS
                     DAHADISISFDEAVTGCDKVIHLQDQNGAMQSLQVHIPAGIDTGKSVRLRGKGNPGIG
                     GGEAGDLFLKVTVGEKQGFERKGNDVYTTINVPFSTAVCGGEAVIKTLYGNVICKIKE
                     GTQSGSKIRLRGKGMPLMKNPSQKGDQYAVVQIQVPRNVSQQAKNKLKEFEALCGQRG
                     NASY"
     gene            complement(14669..15112)
                     /locus_tag="PLC71_01750"
     CDS             complement(14669..15112)
                     /locus_tag="PLC71_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRM01402.1"
                     /translation="MMMPSIFGGNLFNDDWMDFSFPDIDKELYGKHAKNLMKTDVKEK
                     DGNYEVAIDLPGFKKDEITAELKDGYLTISAAKGLDKDEKDKEGKYIRRERYAGNMSR
                     SFYVGKDITEKDIHGKYENGILMLDIPKKAPEKKVEEKKFVTIEG"
BASE COUNT         3986 a         3465 c         2457 g         5433 t
ORIGIN      
        1 cacatgctat ctttcatttt ttctctaaat actcgatgac cttcaactca tacaatttaa
       61 atttaattct tattcaacgt ctgctaatgc tgcaagattt tcttctcctg tacggatttt
      121 aataacttta tctacgttgt atacaaagat cttaccatct ccgatatgac ctgtgtaaag
      181 agctttcttc gcagcttcaa tgatgttttc tactgggata tttcctacga taacttcaac
      241 tttaactttt ggaagtaatg ttgcatcgac ttcagcacct ctgtaacgtt ctccggcacc
      301 tttctggata ccgcagccca taacctgagt gactgtcata ccagttacac caacatcgtt
      361 cattgctttt cttaacttgt cgtatcttgt taattttgcg ataataacaa ctttatacat
      421 tcctgtaggt acaagatctg gagctttgct gacctgtata gctgctgctt tctgtacttt
      481 agaagctctt tcgtaatctt cttctccaag aacagtattt gcattttctt ccatgatcat
      541 tgtgttagaa atatccatca tactgaatcc agcatatgct gatgttaaac catgttctgg
      601 tccatccaga ccgatgatct cttcttcttc tgttacacga agaccaatcg ttgctttgat
      661 aattgtaaat gttaaactga tcgtcacggc tgtccaagcg cagacacaaa caataccgat
      721 gatctgaatc tttaataatc caaatccacc accatagaaa agtccattta attcacttcc
      781 tggtgcagtt tttgttgcaa ataatcctgt tgctaatgtt ccccataaac cgttcataaa
      841 atgaactgct acggctccaa ctgggtcatc aattcttaat ttataatcta ataaccatac
      901 tccaaaacaa acaagtaatc cagaaactgc tccgatcaca attgctccaa aagcatctgt
      961 gacatcacat ggtgctgtga tcgctacaag tcctgcaaga gatgcattta atgacattga
     1021 tacatcaggt tttccatatt ttacccatgt aaataccata caaacaactg ttgcgactgc
     1081 tggtgaaatt gttgttgtta cgaagataga tcctaactgt tcaacagatg ttgctgctgc
     1141 accgttgaat ccataccagc caagccataa gataaatact cctaatgctc caagtggaat
     1201 attgtgtcct gggaatgcat ttacttttgt aatatttcca tctttatctt ttgtgaattt
     1261 accaatacgt ggtcctaaga attttgctcc gatcagtgcg gataatccac caaccatatg
     1321 aattgcacaa gatcctgcga aatcatggaa tcccatctga gataaccatc caccacccca
     1381 gatccaatgt gcttcaattg gatagatcag tgcagagatt actgcagaat aaatacagta
     1441 agataagaat tttgttctct ctgccatggc tccggatacg atcgttgctg ttgttgcaca
     1501 gaatactaag ttgaatacaa aactggacca gtcaaaattt gcataagatg taaaaatgtc
     1561 aaatccaggt tttccaatca atccgatcat atcttccccg agtaataaac tgaaaccgat
     1621 cagaataaat acacaagtac caatacagaa gtccatcagg tttttcatga taatgtttcc
     1681 ggcattctta gctcttgtaa atccggcttc aaccattgca aatccggcct gcatccagaa
     1741 taccaatgcg gctccgatca ggaaccatac cccaaagatc tgggtattta ctgcgttcat
     1801 aatttctttt gtcatatctt tgtcctccca tttcgaaagt ttttgttttt tcattcagga
     1861 gaaacgcgcg tgtctttact caatgaattg attgaaggct tttagaaatc cttcttgata
     1921 aaaagaaaag gcgttatgag gaatgatctg tgtcatctca taacgccttt gttatggttg
     1981 gaattataaa cgccttattt ttatttgtca ataatatttt tagcattttt tatgcaaaga
     2041 atgtatcttt tttagcacaa tcttaactat ttaattcgtt ctcaagcata cggtatccga
     2101 ctccgacttc tgtaaaaaca tactttggaa ctgctggatt tttctcaatc ttgcggcgga
     2161 tatttgccat atttactcgc aaaatcctat tgttcgtatc tgcatgaggt ccccagatat
     2221 ctttgatgat agactcatat gtaataacct ttcctgcatt ctctgcaaga agggatacca
     2281 gtttatattc aatctgtgta agacggattt cttcttctcc aagaaagact cttcgcttat
     2341 caaaatcaat ctttaactga cctgcatgat acattggtgt cttcgtctct gctcccattg
     2401 gctgtcttct atgtcttagg gcagtacgga ttctagcaag cagttcctga ttgccaaatg
     2461 gtttggtgat atagtcatct gctccggcat ccagggcttt tactttctct gattctctgt
     2521 tccttgcaga aatcacgatg atcggagtaa ctgcccattt tctgatctcg gagatcacat
     2581 cgattccatc catatccggt agtccaaggt ctaataagat tacatctgga cagtatgatt
     2641 ccgcacactg gatcgcctga tttcctgtag ttgctatgat tggtttgtag ccattggttt
     2701 ttaagatcgt tgatataaaa ttgccgatgt tttcttcatc ttcagcaatc aatacattaa
     2761 attttatctc tgtcatagtt ccttttcctc cgtcaattct ttttcatcca ttggcagact
     2821 aaaactaaac tctgctccat cctcatagtt tcttgcatag acatcacctt gatgtgccat
     2881 aacgattgat ttacagatac ttaatccgat acccatgccc ttacggcttt catggtctaa
     2941 tgcacctgca cagccgtcaa acaattgatc caagcgctct ggtgcgatac catttccgtg
     3001 gtcacggata tagaatacaa ctttgttccc ttcttctttg atatcaaatt caataggtgc
     3061 atcacttcca gaatgtaccc atgcattttc caaaaggttc ataattacct gctcgatcaa
     3121 agtcggatcc attggaacca ttaaaaaatc ttctgggatc tttaccttta attcactgtc
     3181 tggatatcgc ttctgataac gctggatcgc ttctgctaca acttcttcga gaggttccgg
     3241 tactttcttt acatgtggct gtgtgtcact tccattgctc tggattctcg tgatactaag
     3301 caggttttcc accatgttta acaaccagtt tgcatcttct ttgattccca tgacaagctg
     3361 atcttttgca tcttcatcca gctggtcttt ggcttctaaa tatgtcgaac ttgccccgat
     3421 cattccagtc agcggagtcc taagatcatg tgagatcgct cttaataaat ttgagcgcat
     3481 cttctccttt tctttctcaa tctgaagctc attgatctgc ttgttatatt catttaattt
     3541 tgcaagttct ttctctctct tgacagctgc ttctttctct cggtttaagc ttgttgttgt
     3601 catattcgtg atgattgcga tcactaaaat tccaagaaat gtcatcggat atccgtcaat
     3661 catgaaatta atcttaaaat atggaaacgt aaatctccaa ttaataaaaa taacgcttaa
     3721 cagtgaagaa acaattcccc atccatatcc ttttgtatat cttgagataa aaaaagttgc
     3781 caatgtataa atcattggca cattaatgtt gtgagctgtc tgatagaaaa aaatatgcgc
     3841 aatgatggtc gcaatgatca ggatcgtgat cgagatcaaa aaatccttct cccactgcat
     3901 caaacctttt tcattctgct ccatgtttat tctccattcg gccggatatt cacatctatc
     3961 ttacattttg agacgtgatc agagttcata ctcagatcat aatccacttt aaggtgcatc
     4021 tgttcttctt cttcgatcca gtcaatattg cttgtattga cccccatcaa aagacttcca
     4081 aatggtgtat cgtagtaaga gaggttttct tttccctctt caaagaccat atgagaagac
     4141 tgtcctcctt ttcttgtaag ttccacactc tttggtccga tctttatgac attttttacg
     4201 atctcgttgt cttcagaaag ttcatcgtat aaaatataat gttttccatt tctcaggtaa
     4261 taggcacctg tcgtcatcat ctcgatcgga tcatttggat ctacatccat ctgaagtcct
     4321 gaaatattaa ttaatatatc ttttgtcatc ttatcttcac ttcctaataa ttctcaatat
     4381 ctaccggctg tacctgatcc acacggcatg gaagtacttt gtcggcaaat gataaaaact
     4441 gatccgccat atcactgaca taatattcat acttcgcagt atgatcttct gatgccgcca
     4501 tatctctctg tgcgagcatc tgctttaaag tcttcgcaac ttcataggca gggtttacaa
     4561 gtgtgacctt atcccctaca aatcgctgga tcggattgat caacaatgga taatgcgtgc
     4621 atccaaggat caaagaatca atatcatact gcttaaattc tcttaaatat ctggatacca
     4681 tatcttctgt aatacggtca tcgatcagtc cttcttctac tagcggaaca aataatggac
     4741 atgcttttgt aacgacagtt acagatggat ctagtttcct taaatattgg ccatactgtc
     4801 cactcttgat cgttgcattt gtcccaatga tcccgatatt cttattcttc gtcgcattca
     4861 ttgctgcgat cgcacctggc tgtaccatac cgatgatcgg cacattataa agttcctgta
     4921 aatcagctaa ggcgagcgca ctcgctgtat tacaggcgat gaccactgct ttaacaccct
     4981 tactcaacag aaaattgacg atctgtctgg aatagcgcac gatcgtatct ttggacttcg
     5041 ttccataggg tacacgtgca gtatccccaa aatatatcat actttcttcc gggagctggc
     5101 gcatgatctc tcttgcaaca gtgagcccgc caactccaga atcaaacacc ccgattggtg
     5161 cattcatatc aaattcctcc taacgatatc ttgtcattgc ctgatcgatc aatgtatcaa
     5221 gtaactcagg aatatcaagt ccgcatgctt tccataacat tggatacata ctgatcgatg
     5281 tgaatcctgg caatgtgttg atctcgttaa atacaactct tcctgattct tctaagaaga
     5341 agtcaaccct tgataatcca aatccgtcaa cagctctaaa gattgcttct gcatcttctt
     5401 tgatcttgcg tttagattct tctggcatct ctggatcaac aaccgtttta gactctgcat
     5461 tgttgtattt tgcatcaaaa tcatagaatg ccgcttcttc ggccgcaagg atctctccaa
     5521 cacatgtact cttcacctgt gttctgtcac ctaacaccgc acattccact tctcttccga
     5581 caattgtttc ctcacaaagg atattacggt catgtttcac tgcttcgtat aatgccgctt
     5641 ctaattcttt tctattctct gctttggaaa ctcccttaga agatcctgca caagatggtt
     5701 ttacgaagat cggatatgga atttcctttt ccacacgatc catggcttct tctaaatgtt
     5761 caaaatcgct ttctctgaca tgaacaaact ttgcctgatc gattccaata tgatcgacga
     5821 tgatctttgt gtatacttta tccattgaaa ctgctgatgc caatacacca catccaacat
     5881 atggaatttt agacaattca aacagtccct gaacggttcc atcttcacca ttcatcccat
     5941 gaagaactgg gaagatcaca tcaactttct gaagtttata tgtaccttct gcaaggatca
     6001 ctaaagaacg agttgttgta tcaggagaaa taaatgcttt cacttctcct tctctccaac
     6061 ttccatcttc aatatcttta acaccatcta ctaataacca tcttccttct tttgtgatac
     6121 caactaagat cacattatat ttttcagtgt ccatgttctt gatgactgtc gttgctgaca
     6181 gacatgaaac atcatgttca gaggaatctc ctccaaagat cactactaca ttctttttca
     6241 ttgatttaaa ctcctttatc atcataaaaa gtatttcatc attatcattt gattatagca
     6301 caaatttttc aaaaataaat ccttcaaaat aaggttcatt ctctttatat gcttttttat
     6361 cgcgaaatgt ccatccgtaa attggaatct tgtaaaatct gcggcagatc ttgagtggaa
     6421 gacagtcttt atatttatag tgataggcta tgaaatctgg ttttgtaata aaatttgtca
     6481 ataaattctt cacaataaaa taaccgattt tgcgagtaaa cgtcccttct tctttttctt
     6541 tcaaaaactg ctcacttaac tgtccacgga tcacttctgg acggtttttc ttaaaccaat
     6601 acagagcata cggatgaaaa cattccatac aatataatcc tttatattga tctaaagctt
     6661 ttgcaacttt cttgcataat ttacggttgc atgtttccat ttttaattct actaataatg
     6721 gtactttgcc atcaacttct cttaaaactt ccacaaacct tggaattctt tctctcgttc
     6781 ccatcaatcg atatctgcat aattcacgat aagtaagatc tgtgatcttt ttatttactc
     6841 cacatgttct cttaagatca tagtcatggt gaactaccat tacaccgtct ttggtcagct
     6901 gaacatctaa ttctattcca tagcctgatc tctttgcttt cttgaaagct gccatggaat
     6961 tttctggaat tccttcttct ccatgaaatc ctctgtgtgc atagtatttt acatttaata
     7021 ccggatgttt tcttttcttt cccggatcga tcatcttgat gtatacaata attaagacca
     7081 atatccataa caggatcatt tctcatccct cttttcttct gccatccgcg ttaatgtttc
     7141 gcgttcattt aactgtggat cttctaatac ctgatctaac agccaggata aaatttgacc
     7201 gatttctttt ccctgtggaa ctcccatatc catcaagtct tttccattga tctttaattc
     7261 tttgatggaa aggcagtttt tctcttcgat gatttcttcg aataatttct ttgtttcttg
     7321 taatacctgt tctttcttat ctctctgata atcactttgt gcactgatat ctgctttctg
     7381 gacctccatc agtcttgtaa acaactctgg ggttaactgg ctgacccatc gtctgactgt
     7441 tttcttattg acttcttcta tagaagcaaa tctacggtca tgccatctga caagagtcgt
     7501 tacctgcttg atcgtctggt tgtcaaactt taatcttcgg aggatcttcc ttgccatctc
     7561 ttctccgatc accggatgca ttttaaaatg atctttatct ggaccttcaa ctctcgctgt
     7621 tggttttccc acatcatgaa gcagcattgt ccaccggtaa atatgttctg ctttgatctg
     7681 tttcattgct tcgatcgtat ggattccaac attgtatttg tgatgtggat tattctgcgg
     7741 tgtttccatc ataatatcaa attctggaaa aatgatcttc gtcacaccta gtttatatgc
     7801 attgaccaat acttctggat gtgccgatgt gatcagtttc tcaagctcta cctgaattcg
     7861 ctcagcaatg atatctttta ggaattttgc ctggttttta attgcttctt cggtttcttc
     7921 ttcgatctga aatcctaatt gagcctgaaa tcttagggct cttaaaattc tcaatgcatc
     7981 ttcatcaaat cgtttctttg cttccccgac acaacggatc gtttgatttt taaggtctgt
     8041 cataccatca tacatgtcaa tgatcccctc actgtcatta tatgccatcg cattgatcgt
     8101 gaaatctctt cgtaacagat cttctcttaa tgactttgta aaatgcactt cattgggacg
     8161 tctatgatct tcgtattttc catcgacacg ataggttgtt acttcgtatg gttcatgatt
     8221 cataagaacc gtaactgttc catgttcgat tcctgtatct accgtatgat cgaagatttc
     8281 tttgacttcc tgaggtgaag ctgatgttgt gatatcccag tcacccggcg ttcttcctag
     8341 tatggaatct ctgacgcagc ccccaacgat atatgcctca aatccatgtt gttctaatgt
     8401 ctgtattacc tttgctgcct gtgcgggcat cttgatcttc atcctatcac ctctcctttt
     8461 ctttgttctt atatctgctt ctcatattta tttactattc ttacaaatgt atctcgcaaa
     8521 cggaagaatt gccggaaatg tatcttcata atccccggca attgttatca ttatactctt
     8581 agtaagcttt tccaaagtaa accatcttct tcgcaggttt tccacagtga acacatacgt
     8641 cacttaaatg ttcctgctca aatggcatac atcttgttgt agctgttgtc tcgtctttga
     8701 tcgcttcttc gcattctgtt tctccacacc acattgcttt tacgaaacca tcctgtttct
     8761 cgatgtgggc tttgaattca tcccagtttc tagcctctac tgtatgttct tccagatgtt
     8821 tctttgctct ctcaagcata tcgttctgca tctgttctaa tacttcacca agttttgtct
     8881 cgatctcatc taaagataca atgatcttct ctcttgtatc acgacgaacg atcaccgcct
     8941 gattcttctc gatatctttt ggtccgatct cgattcttgt aggaattccg cgtacttcct
     9001 gttcactgaa tttccatcct gggctcttat cagaatcatc aacttttact ttgtaagaag
     9061 aagcaagtct ctttctgatc tcttctgctt tatcaagcac tccttctttc ttctgcatga
     9121 tcggtacgat cattgtctgt actggtgcga ttcttggtgg taatacaaga cctgaatcat
     9181 ctccgtgaac catgatgatc gctccgatga tacgtgtaga cattccccat gatgtctgat
     9241 gtacatactg aagtttatta tctttatctg tatactgaat atcaaatgct ttcgcaaatc
     9301 catcaccaaa gttatggctt gttccagact gtaatgcttt tccatcgtgc attaatgctt
     9361 caatcgtata tgtggaatgt gcacctgcga atttctcttt ctctgtcttc tgtcctttta
     9421 caactggaat tgccagatac tgctcacaga aatctgcata catatttaac atctgtaccg
     9481 ttcttgcttc agcttcttct tctgtcgcat gtgctgtatg tccttcctgc cagaagaact
     9541 ctgaagttct taaaaatggt cttgttgttt tttcccaacg tacaacagat acccactgat
     9601 tatataattt aggtaaatct ctgtgagact ggatgatctt tgaatagaaa tcacagaata
     9661 atgtctcaga tgtaggtctt acacaaagac gttcctgtaa ttctttatct ccaccatgag
     9721 ttacccatgc aacttctggt gcaaatcctt cgacatggtc ttcttctttc tttaaaaggc
     9781 tttctggaat aaacattggc attgcaacat tctccacacc tgtttcttta aatttggcgt
     9841 ctaaaacttt ctggatattc tcccagatcg catatccatt cggacgtaag atcatacatc
     9901 cacgaacgct tgaataatct gctaattctg ctttcttaac aacatctgta taccactgtg
     9961 caaaatcatc ttccatcgca gtgatcgctt ctactaattt cttatctttt gccattgcaa
    10021 aatgtctcct tttctatggt tatatacaca gtttttgtgt acaatgatac taagaataat
    10081 attactgtaa aatgtcggat ttttcaaggg gtgacgtttt atttaactac acaaaaagga
    10141 gttttttatg gcaaatgaaa acaagatggg gtatcttccc atcaaaaagc tgatattgca
    10201 gatgtccctt cctccgatgt gttccatgtt tctgcaatat tcttacaacc ttgttgacag
    10261 catgttcgtg gctaatatca atgaaaatgc acttgcggca gtttctcttt ccttcccgat
    10321 cacaaccctt atgatctccc tgtccatctg gattggggtc ggtatcaacg ttttgatcgc
    10381 aggttatctt ggagagaaag aacaagacct tgctgactgt gcaacaacac ttggattgtt
    10441 ggtagccttg atcgtcggta tctgtatcaa tatccttgca ctattgatca tgaaaccgta
    10501 cttttcagca ttcacaaaca atccagctat ttttgactat gcgatgcagt atatgtccgt
    10561 ctgtgctttt atgcaggttc caaacatggt acatatcgcc atccaaaagg tctttcaggg
    10621 tactggaaat atgatcactc ctatgtggtt tcagatcgca ggagttatct ttaactttgt
    10681 tttcgatcca ttactgatct ttggaatcgg accattccct aagatgggaa tccgtggtgc
    10741 tgcactggct actgtccttg gatatgcttt atctatggtg attgcttttc ttctgcttgt
    10801 ttttacaaaa cagaaagttc gtatcaagat caaaggattc catgttgatt ctcatatgtt
    10861 aaagaatctg ttttcctatg gatttccttc atttgttatg aatgctcttg gatcttttat
    10921 gctgacattt accaatctgt ttttaacagc ttattcagat actgttgttg ccttttttgg
    10981 cgcctatttc aaagtacaac agatgatctt catgacagta aatggcttga tccaaggatg
    11041 tttgcctgtc atgagattca actacagtgc caaacagcca gaacgtttaa aagaagcttt
    11101 tcattttggc acacaggcgg caactgttat gatgatcggt ggaacatgcc tgcttctgtt
    11161 tttaccaaaa cagatcttgt tcctgtttgc agcttctgaa tcaatgatca aaatcggagt
    11221 tccagccatg cggatcctgt cgatcggttt agttttctgt ggattttcta ttatgatcgc
    11281 tacttatcag caggcaattg agaagatcca atctagtatg ttgatccaca tcttacggca
    11341 gggaatttta ttggtgcctt tgatgtggct gtttaatcat atgtttggga taaatgggat
    11401 ttggatctca tttcctgtaa cagaaatttt agtgtttatt gtggctgttt tattacaaac
    11461 tgttatttca aaaccacaat aaaaattatt atttcttgta ttgaaaaagc tcactcctgt
    11521 aaaaataaaa aaggtagcag attccttttc cgaagaaatt ggaatctgct accttacatt
    11581 ttatacttta tcacattttt aaatattaca gtaaagattt atataatcca ataatatcct
    11641 caacacttgc atccttaggg tttccaggtg cacatgcatc tgcataagca gattctgcta
    11701 agaactggat atcttcttct ttcacaattt cttttaagtc tgctggtatt ccgacatctg
    11761 ctgataattt tttaaccgca tcaacggctg cttttctgta ttcttcctgt gtcatatctt
    11821 cgacaccttc tacacccatt gcttttgcga tatatttgta tttatctcct gttgcatctg
    11881 cattgtattc cataactgtt ggaagtaaga tcgcattcgc tactccatga ggtgtatcat
    11941 aaactgcacc taatgcatga gccatagaat gtacgattcc aagtcctaca tttgagaatc
    12001 ccattcctgc aatgtactgt cccattgcca ttccttctct tccttctggt gtattctcaa
    12061 cagctcctct taagctgtca gagatcagtt cgatggcttt gatatggaac atatctgtca
    12121 tttcccaagc tgcttttgtt gtgtatcctt cgatggcatg tgttaatgca tccatacctg
    12181 ttgctgctgt taatcctttt ggcatagaag acatcatttc tgggtcgatc actgcgatca
    12241 ctggaatatc atgagggtct acgcatacga atttacgttt cttttcaacg tctgtgataa
    12301 cgtagttgat cgttacttct gcagctgttc cggctgttgt tggaactgcg atgattggta
    12361 cgcaaggttt cttagtatct gcaactccct ctaagcttct cacatcttca aattctgggt
    12421 tgttgatgat gattccaaca gcttttgctg tatccataga agatccacca ccaatagcga
    12481 tcaggtaatc agctcctgct gccttaaatg ctgcaactcc tgtctgtacg ttttctatcg
    12541 ttggatttgg tttgatctgt gaatatactt catatgcaag tcctgcatta tctaatacat
    12601 ctgttacctt ctttgtaact ccgaatttta caagatctgg atcagaacaa acaaatgctt
    12661 ttttgaatcc acgttttgtt gcttctgttg cgatctcctg aattgctcct gctccgtgat
    12721 aagatgtttc atttaataca atacgattta ccattttgta cactcctttt atattgacat
    12781 ttttttaact atcttcagta taaaattgtt ttttcacaaa tacaagttac acatttggca
    12841 aaatgtaaaa aatgttacac ttcatcaaaa gtgtaacatt ttgttatctt tatatatctt
    12901 tgaacagatc atatagcttt ggaggcatcg catcgatcag ctgatctgca agctgttccg
    12961 gcgacatctt catccctgtt gtcgcccatt ctacagtcat ggcaattgaa cctcggcaat
    13021 acattctcaa taagaattct aattcttctg gaattttatc aactccctgt ttgtgaatga
    13081 tatcacaata gaattgataa atgcactgat aatcatactc catcagacag ttctgactct
    13141 cgctcgaaaa agctgctgcg aaaaattgtc cttcaccttt gatgaattca aatttcttga
    13201 gcaacccttc tcttaatgtc agactgatac ccatctgttt aaacgacatc tgagccaata
    13261 catcaaaata ccagttgatc agatcatatt tatccttaaa atgccgataa aaggttggac
    13321 gagttacatc gcactgatct acgatctgtt tcactgtaat cttatctact ggcgtatgca
    13381 ccaacaactc tttcattgca tttgctaatt tatatttcat tttctctgat acgtccatct
    13441 cgtcccacct tctttcatat atggttttat tgtaccttat ttgacacaat ttataaaggc
    13501 aaaaaaggct atcatctgct tttgtctcca aataacaacc ttttttatct attatcaaga
    13561 agtttcatgt ttaataagag gcatttcctc tttgaccaca aagtgcttcg aattctttta
    13621 atttgttttt tgcctgctgg gagacatttc ttggaacctg aatctgaaca actgcgtatt
    13681 gatctccctt ctgagatgga ttcttcataa gcggcattcc ttttccacgc agtctgatct
    13741 tagatcctga ctgagttcct tctttgatct tacagataac atttccatat aatgtcttga
    13801 tcactgcttc acctccacaa acggctgtgc taaatggaac attgattgtt gtatacacat
    13861 catttccttt tcgctcaaat ccttgtttct caccgactgt tactttcagg aataaatcac
    13921 ctgcttcacc gcctccgatt cctggatttc cttttcctct taagcggaca ctttttcctg
    13981 tatcaattcc ggctggtata tgaacctgta atgactgcat cgcaccattt tgatcttgca
    14041 aatgaatgac tttatcgcag cccgtaaccg cttcatcaaa actaatagag atatctgcgt
    14101 gtgcgtcgga acctttctgt ctgaaggatc cacttccgaa tccaccacct ccaaatccat
    14161 cagaagagcc aaaacctcct tgatggaaac tgcttcgatg gaatcctttg ttgtttcttc
    14221 cgccaccgaa catacttcca aagatgtcac caaagatatc atcgccatct ccaccttcaa
    14281 agtggaattc ctgatatcct ccatgagcac caccatagtt ttggtaattc ttatagaact
    14341 cagattctgc tccggttccg tcaaaggcag catgaccata ttgatcatat aattttctct
    14401 tctctggatc acttaatata ttataagcat cggtcgcttc tttgaatttc tgttctgctg
    14461 ttttatctcc gggattcata tctggatgat atttctttgc cagtttccga tatgctttct
    14521 tgatcgtttt ttcatctgca gttttactga ttccaagaac ttcatataaa tcacgttttg
    14581 ctgccatttc gtttcacctt cttttccttt cattctcaaa tagatagaaa gattccccgg
    14641 gaatctgatc atcccgggga aagttctttt atccttcgat cgttacgaat ttcttttctt
    14701 ctactttctt ttccggagct ttcttcggaa tatccagcat cagaatacca ttctcgtatt
    14761 taccgtggat atctttctct gtgatatctt tgccaacata gaagcttctg ctcatgtttc
    14821 ctgcgtaacg ttcacgacgg atatatttac cttctttatc tttctcgtct ttatctaatc
    14881 cttttgctgc actgatcgtt aaatatccgt ctttcagttc agctgttatc tcatctttct
    14941 taaatcctgg aaggtcgatc gctacttcgt agtttccgtc tttttccttc acatcagtct
    15001 tcattaagtt ctttgcgtgt ttaccataca gttctttatc aatatctggg aaagaaaaat
    15061 ccatccagtc gtcgttaaat aagtttcctc caaaaatact aggcatcatc atcataagtc
    15121 atctctcctt tttctttata ataatgtttg ggcgtgtcag ggtaattcct gacacttgcc
    15181 ttttatgtga agcatattga gaagttctca atatgacttg ttgtatctta tggagccttc
    15241 ggttctttgg cttctctctt aagcccttcc ctcttgctct agttgtacta taacacctat
    15301 tattagcact gtcaataggt gagtgctaat tttttgagat t
//
LOCUS       DAOMUE010000017        15233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_809_length_15233_cov_3.104889, whole genome shotgun sequence.
ACCESSION   DAOMUE010000017 DAOMUE010000000
VERSION     DAOMUE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 15233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15233
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_809_length_15233_cov_3.104889"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /gene="rpsH"
                     /locus_tag="PLC71_01755"
     CDS             <1..156
                     /gene="rpsH"
                     /locus_tag="PLC71_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRM01403.1"
                     /translation="KPGLRVYAGAEELPKVLGGLGIAIISTNKGLLTDKEARKQNVGG
                     EVLAFVW"
     gene            244..786
                     /gene="rplF"
                     /locus_tag="PLC71_01760"
     CDS             244..786
                     /gene="rplF"
                     /locus_tag="PLC71_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRM01404.1"
                     /translation="MSRIGRLPIDIPAGVEVKIEEGNKVTVKGPKGTLEKCLPVEMTI
                     KQEDNQVVVTRPNDLKKMKSLHGLTRSLIANMITGVSEGYEKKLEINGVGYRAQKKGK
                     EITFNLGFSHPVVMTDPEGIETEMDGQNIIIVKGIDKEKVGQYAAEIRELRKPEPYKG
                     KGIKYADEVIRRKVGKTGAK"
     gene            801..1169
                     /gene="rplR"
                     /locus_tag="PLC71_01765"
     CDS             801..1169
                     /gene="rplR"
                     /locus_tag="PLC71_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRM01405.1"
                     /translation="MIRKESRSKIRAKKHYKLRNRINGTSERPRLSVFRSNDHMYAQI
                     IDDTVGNTLVSASTTQKDVKAELEKTNDVAAASYLGKVIAERALAKGITEVVFDRGGF
                     IYHGKIEALADAAREAGLKF"
     gene            1184..1693
                     /gene="rpsE"
                     /locus_tag="PLC71_01770"
     CDS             1184..1693
                     /gene="rpsE"
                     /locus_tag="PLC71_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRM01406.1"
                     /translation="MKHTLIDPSQLELEERVVSIKRVTKVVKGGRNMRFAALVVVGDK
                     NGHVGAGLGKAAEIPEAIRKGKEAAMKSLVEVPIDEKGTTPHDFTGNFGGASVLLKTS
                     PEGTGIIAGGPSRIVLELAGYKNIRSKSLGSNNKQNVVLATIEGLKNLKTPEQVAKLR
                     GKSVEELLG"
     gene            1707..1889
                     /gene="rpmD"
                     /locus_tag="PLC71_01775"
     CDS             1707..1889
                     /gene="rpmD"
                     /locus_tag="PLC71_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRM01407.1"
                     /translation="MADKLKITLVKSTIGAIPKHKKTVEALGLKKLHKTVELPDNAAT
                     RGMIHQVKHLVKVEEI"
     gene            1917..2360
                     /gene="rplO"
                     /locus_tag="PLC71_01780"
     CDS             1917..2360
                     /gene="rplO"
                     /locus_tag="PLC71_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRM01408.1"
                     /translation="MELSNLRPAEGSKHSDAFRRGRGHGSGNGKTAGKGHKGQKARSG
                     GGVRPGFEGGQMPLYRRIPKRGFKCRNSKEIVGINLSALERFEDGAEVSVEALIEAGI
                     VKNPRDGVKILGNGELTKKLNVKANAFSASAKEKIEALGGKAEVI"
     gene            2362..3678
                     /gene="secY"
                     /locus_tag="PLC71_01785"
     CDS             2362..3678
                     /gene="secY"
                     /locus_tag="PLC71_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRM01409.1"
                     /translation="MSNAVVSAFKNKQLRKKLLFTTLILIVVRFGSQLPIPEIDSAQI
                     SAYLKSTLGDSFSLLNSFTGGSFMQMSVFALSVTPYITSSIIMQLMTIVIPALEEMQK
                     DGEDGRKRMAKITRYVTVVLAIIEGAGLAIGFANQGALGTDYTTFTIVTMIIALTAGA
                     VLVMWLGERITESGIGNGISIILLVNIVSGMPGDFTSLYNQFMKGKQIGPALIAGCVI
                     VGVVLAVVVFVIVLSDAERHIPVQYSKKMQGRKLVGGQQSKIPLKVNTAGVIPIIFAS
                     SIMQFPIMLQNVLKYENNGFIGKALTSLNSSTWFDASHPKRSIGLLIYIVLVVLFAYF
                     YTSITFNPLEISNNMKKQGGFIPGIRPGKPTVDYLNKILKYIIFIGAAGLTIVAVVPF
                     FFNGVFGASVSFGGTSIIIVVGVILETIKQIQSQLLVQNYSGFLSE"
     gene            3832..4473
                     /locus_tag="PLC71_01790"
     CDS             3832..4473
                     /locus_tag="PLC71_01790"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRM01410.1"
                     /translation="MKIIMLGAPGAGKGTQAKQIAQAYDIPHISTGDIFRANIKEGTE
                     LGKKAQEYMDKGLLVPDELVCDLVVDRIHKDDCEKGYILDGFPRTIPQANALDEALAK
                     DGEKIEFAIDIEVPDENIINRMSGRRSCKDCGAIFHVQYNPPKKENCCDVCGGELILR
                     DDDKAETVKKRLDVYHEQTAPLIAHYKQAGSLHEIDGTQDIKVVFEEIKSILG"
     gene            4484..5239
                     /gene="map"
                     /locus_tag="PLC71_01795"
     CDS             4484..5239
                     /gene="map"
                     /locus_tag="PLC71_01795"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRM01411.1"
                     /translation="MAVTIKSKKEIELMREAGKMLEEVHNKLADFVKPGISTLEIDQF
                     GEKAIRDLGCVPNFLNYNGYPASICVSVNDEVVHGIPSKDRILQDGDIVSLDAGLIHE
                     GYHSDAARTHAVGNISEEAKRLIEVTKQSFFEGIKLAVPGNHLFDISEAIQKYVESNG
                     YSVVRDLVGHGIGTHLHEDPQIPNFKQRRKGMKLRPGMTLAIEPMVNEGSYDVVWLDD
                     DWTVVTEDGSLAAHYENTIVITEDGCEILTLSE"
     gene            5250..5468
                     /gene="infA"
                     /locus_tag="PLC71_01800"
     CDS             5250..5468
                     /gene="infA"
                     /locus_tag="PLC71_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRM01412.1"
                     /translation="MAKSEAIEVEGVVLEKLPNAMFKVEIEGGHVILAHISGKLRMNY
                     IKILPGDKVTLALSPYDLSKGRIIWRDK"
     gene            5567..5680
                     /gene="rpmJ"
                     /locus_tag="PLC71_01805"
     CDS             5567..5680
                     /gene="rpmJ"
                     /locus_tag="PLC71_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRM01413.1"
                     /translation="MKVRASVKPICEKCKVIKRKGSVRVICENPKHKQRQG"
     gene            5955..6323
                     /gene="rpsM"
                     /locus_tag="PLC71_01810"
     CDS             5955..6323
                     /gene="rpsM"
                     /locus_tag="PLC71_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRM01414.1"
                     /translation="MARISGVDLPREKRVEIGLTYIYGIGLSSAKRILAEANVDPDTR
                     VRDLTDDEVKRISAVINDTQVVEGDLRREIALNIKRLKEIGCYRGKRHRQGLPCRGQK
                     TKTNARTRKGPKKTVANKKK"
     gene            6446..6838
                     /gene="rpsK"
                     /locus_tag="PLC71_01815"
     CDS             6446..6838
                     /gene="rpsK"
                     /locus_tag="PLC71_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRM01415.1"
                     /translation="MAKVTKKSAKKRVKKNVQHGQAHIQSSFNNTIVTLTDAQGNALS
                     WASAGGLGFRGSKKSTPYAAQMAAETATKAALVHGLKSVDVMVKGPGSGREAAIRALS
                     AAGLEVTSISDVTPVPHNGCRPPKRRRV"
     gene            6858..7451
                     /gene="rpsD"
                     /locus_tag="PLC71_01820"
     CDS             6858..7451
                     /gene="rpsD"
                     /locus_tag="PLC71_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRM01416.1"
                     /translation="MAVDRTPVLKRCRSLGLDPVFLGIDKKSNRSLNRANRKKSEYGL
                     QLCEKQKAKFIYGVLEKPFRNYYAKAERMKGQTGTNLMTLLELRLDNVLFRLGYGRTR
                     KECRQIVDHKHVLVNGKQVNIPSYQVKAGDVIEIKEKHRTSPRYKEILEVTEGRMIPS
                     WLEADHDNFTGTVKELPSRDEIDVPVNETLIVELYSK"
     gene            7491..8447
                     /locus_tag="PLC71_01825"
     CDS             7491..8447
                     /locus_tag="PLC71_01825"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRM01417.1"
                     /translation="MFEFEKPNIEINEISEDNRFGRFVVEPLERGYGTTLGNSLRRIM
                     LSSLPGAAVSSVKIKGVLHEFSSIPGVKEDVPEIIMNIKSLAIKNNSSSAETKQAYIE
                     FSGKGVVTAGDIKADSDIEILNPELVIATLSDDSSKLDMELTITNGRGYVGADKADKE
                     GKAIDEIAVDAIYTPVERVNLKVENTRVGQITDYDKLTLDVFTNGTLTPDESVSLAAK
                     VLCEHLNLFVDLSENAKTAEVMVEKEDDEKEKVLEMSIDELELSVRSYNCLKRAGINT
                     VEELTNKTSEDMMKVRNLGRKSLEEVLAKLSELGLSLSSGEE"
     gene            8513..9049
                     /locus_tag="PLC71_01830"
     CDS             8513..9049
                     /locus_tag="PLC71_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HRM01418.1"
                     /translation="MAKYRKLGRTASQRKALIRAEVTNLLHHGKIVTTEAKAKEIRKV
                     AEKLIALAVKEKDNFETVTVDAKVAKKDENGKRVKEVVDGKKVTVYETVQKEIKKDLP
                     SRLHARRQMLKVLYPVTEVPTEAAGKKANTKKVDLTAKLFDEYAPKYAERNGGYTRIV
                     KIAQRKGDAALKVVLELV"
     gene            9195..10046
                     /locus_tag="PLC71_01835"
     CDS             9195..10046
                     /locus_tag="PLC71_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRM01419.1"
                     /translation="MSILKIKDLVHKYVTYGDTEEDKQEIAAIDHIDINVEKGEFVAI
                     LGKNGSGKSSLAKHINGLLMPTEGTVYIKGMDTKDESTLLKVRQSAGIVFQNPDNQIV
                     GTTVEEDTAFGPENLGIASKDIWTRITAALTGVGMIAYKEASPNHLSGGQKQRVAIAG
                     IMAMEPECIVLDEPTAMLDPEGRRHVLSLVKALNKEKNITILMVTHHMEEVILADRII
                     VMDQGKIVMDGKPKEIFSRVDEMKTLGLEVPYATELAWELKKEGVNLSDTITTKEELV
                     EELCQFV"
     gene            10031..10891
                     /locus_tag="PLC71_01840"
     CDS             10031..10891
                     /locus_tag="PLC71_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRM01420.1"
                     /translation="MSIRLEHVSYAYEDNSSKKNYALKDINLEIKKEEFIAVIGHTGS
                     GKSTLIQHLNGLIKPTSGTIYFDDEDIYSEGYPLRELRGKVGICFQYPEHQLFETTIL
                     DDVCFGPMNFGKTKEEATEIAVKCLKDVGLPEKLYQKSPFELSGGQKRRVAIAGILAM
                     EPEYFILDEPTAGLDPVGKDQILNLLKKLHEEKKITIILVSHSMEDVADYAQCVIVMN
                     GGQVMYEGDKKEVFSHKKELEEAGLAVPFYRQVCEELADRGFPIQRDLLTLEETKEAI
                     LEGLKGLRGK"
     gene            10893..11702
                     /locus_tag="PLC71_01845"
     CDS             10893..11702
                     /locus_tag="PLC71_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRM01421.1"
                     /translation="MLKDITIGQYYKEDSLIHRLDPRVKLFAVLIYVITLFMHKAPAT
                     YVMSLLFLIITIKISKVPVKYILRGLKAIAVILLFSVMINMLFIPGKPVIQFWIISIS
                     AEGIRTTIYLGSRLIMLVLGTSLLTFTTTPNELTDGLDKSLGFLNKVGVPVHEIAMMM
                     SIALRFIPILTEELEKIMKAQTARGIDFESGGLLKRVKSMVPIIVPLFVAAIRRANDL
                     AMAMESRCYHGGVGRTKLKPLKYEKRDKIAYIVLFVFLAVMIYLSFFSPWR"
     gene            11709..12467
                     /gene="truA"
                     /locus_tag="PLC71_01850"
     CDS             11709..12467
                     /gene="truA"
                     /locus_tag="PLC71_01850"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRM01422.1"
                     /translation="MKRVKLIVAYDGTGYCGWQVQPNEITVEGVLNKHISELTGEDIK
                     VIGASRTDSGVHALGNVAVFDTESRIPGEKMAKALNARLPEDIIIQDSKEVPLDYHPR
                     FQDTRKTYEYTFYNARYDNPVTSRHHHFVYVPLDVEKMKEAASYFLGEHCFISMCSSK
                     AQVTSYVREIYECDVTQNGRYITMRVTGSGFLYNMVRLMAGTLLEVGRGKQEPQWVKE
                     ILASKERVTPGPKLPAKGLTLIQIEYPEDRENAE"
     gene            12457..13398
                     /locus_tag="PLC71_01855"
     CDS             12457..13398
                     /locus_tag="PLC71_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM01423.1"
                     /translation="MQNSFIIFQKLLVLFGFMLIGYLSYKKKWISDDTSSQISGLIVN
                     IFNPALIISGVIGSVGNGNWNLVIMDLILAVILFVVLILISPAFVRILSVKKDERNIY
                     AVMLIFSNLGFMGIPIIEELYGRGAIFYVALYTLVYNILFYTYGIYLFEKERAMQTGQ
                     KAKIIFHWKKMINPGMVACLAALLIFAFQIDAPAPAVSFVQYLGNAAIPLSMIITGVS
                     LAKMPLIEVFKDIKMYQFTFLKMLVIPMIAAFAIRLFHLDPVLSGIMVLMFGMPNGSM
                     AVMMAIDYGLDSSICSRGIVLTTLLSIITLPIVAYLI"
     gene            13656..14090
                     /gene="rplM"
                     /locus_tag="PLC71_01860"
     CDS             13656..14090
                     /gene="rplM"
                     /locus_tag="PLC71_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRM01424.1"
                     /translation="MKSFMASPSTIEREWYVVDATGHTLGRLASEIATILRGKNKPTY
                     TPHIDTGDNVIVINAEKIKVTGKKLDQKIYYHHSDYVGGMKEQTLKEKLAKKPEDVIY
                     LAVKGMLPKGPLGRQMIKKLHVYAGAEHNHAAQQPKALEIKY"
     gene            14112..14504
                     /gene="rpsI"
                     /locus_tag="PLC71_01865"
     CDS             14112..14504
                     /gene="rpsI"
                     /locus_tag="PLC71_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRM01425.1"
                     /translation="MATAKYYGTGRRKHSVARVYLVPGTGKITINKRDIDEYLGLETL
                     KVVVRQPLVATDTIDKYDVLVNVHGGGFTGQAGAIRHGISRALLQVDGEFRSTLKKAG
                     FLTRDPRMKERKKPGLRKARRAPQFSKR"
     gene            14627..14755
                     /locus_tag="PLC71_01870"
     CDS             14627..14755
                     /locus_tag="PLC71_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01426.1"
                     /translation="MLSYISKARSGSGIFNAEKMSVYVPLGTQQMLSDYGYHIVTE"
     gene            14773..>15233
                     /locus_tag="PLC71_01875"
     CDS             14773..>15233
                     /locus_tag="PLC71_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01427.1"
                     /translation="MKLKNLTKKLTTLAITALMATTLVSTQNVLAASKKAQKKANKAY
                     AQAIKKNKVAKFTSYSLIEVTGGGTKELVTVNLNSNAEGEARNLNIYTYNKGKVKKIW
                     STSDQIIAYNKKQKTIAGLQYSVLSENGSEREKYYIYKYDGKKIVKKASYF"
BASE COUNT         5605 a         2400 c         3283 g         3945 t
ORIGIN      
        1 aaaccagggc ttagagtata cgcaggggct gaagagcttc ctaaagtatt aggaggactt
       61 ggtattgcga tcatctctac aaacaaagga ctgttaacag ataaagaagc aagaaaacag
      121 aacgtgggtg gagaagtgtt agcttttgta tggtaagatt tactgtacat taaccagcat
      181 tcactattct gaaaacagaa gggacacacc cttgtccctt ccgacaattt aaggaggtaa
      241 cctatgtctc gtataggtag attaccaatc gatatccctg caggagtaga ggtaaagatt
      301 gaagaaggaa acaaagtgac tgtaaaaggt cctaaaggaa cattagaaaa atgtttacca
      361 gtagaaatga caatcaaaca ggaagataat caggttgtcg taacaagacc aaatgactta
      421 aagaaaatga aatctttaca tggtcttaca agatctttga tcgcaaacat gatcacaggt
      481 gtatctgaag gatacgaaaa gaagcttgaa atcaacggtg ttggttacag agcacagaaa
      541 aaaggaaaag aaatcacttt caaccttggt ttctctcatc cggtagttat gactgatcca
      601 gaaggaatcg aaacagagat ggacggacag aacattatta tcgttaaagg tatcgacaaa
      661 gaaaaagttg gacagtacgc agctgaaatc agagaactta gaaaaccaga accttacaaa
      721 ggtaaaggta ttaagtatgc tgatgaagtt atcagacgta aagttggaaa aactggggct
      781 aaataaagga gtgatatagg atgattagaa aagaatcaag aagcaagatt cgtgcgaaaa
      841 aacactacaa gcttcgtaat cgaatcaatg gaacaagcga aagacctcgc ttaagtgtat
      901 ttagaagtaa tgatcatatg tacgctcaga ttattgacga cactgttgga aatacattag
      961 tatctgcttc aacaactcag aaagacgtaa aggcagaact ggaaaaaaca aatgatgttg
     1021 ccgcagctag ctacttaggt aaagtaatcg ctgaaagagc tttagcaaaa ggtatcacag
     1081 aagttgtctt tgacagaggc ggatttattt atcatggtaa aattgaagca ttagcagatg
     1141 cagctcgtga agctggtctg aaattctaat aggaggaagt caaatgaagc atacacttat
     1201 tgaccctagt cagttagagc tggaagaaag agttgtatcc atcaaacgtg ttactaaagt
     1261 agtaaaaggt ggacgtaaca tgagatttgc agcactggtt gtagtagggg ataaaaatgg
     1321 tcatgttggt gctggattag gaaaagcagc tgaaattcca gaagcaatcc gcaaaggaaa
     1381 agaagcagct atgaagagcc ttgttgaagt accaatcgat gaaaaaggaa caacaccaca
     1441 cgactttaca ggaaacttcg gtggagcatc tgtattatta aaaacatctc cagaaggtac
     1501 tggtatcatc gctggtggac cttcccgtat cgttctggaa cttgcaggat ataaaaacat
     1561 ccgttcaaaa tctttaggtt ctaacaacaa acagaacgtt gtattagcaa caatcgaagg
     1621 attaaagaac ttaaaaactc cagagcaggt agcaaaacta cgtggaaaat cagtagaaga
     1681 gctcttagga taggaggaat tttgaaatgg cagataaatt aaaaatcaca ttagtaaaat
     1741 ctacaattgg tgcaattcca aagcacaaaa aaactgttga agcattaggt cttaaaaaac
     1801 ttcacaaaac agttgagctg cctgataacg cagcaacacg tggaatgatt caccaggtga
     1861 aacatttagt taaagtagaa gaaatctaat aatattcggc aaggaggtgt aacggaatgg
     1921 aattatcaaa cttacgccct gcagaaggat ctaaacatag cgacgctttc agaagaggtc
     1981 gtggacatgg ttcaggaaat ggaaaaactg caggtaaagg acataaagga cagaaagctc
     2041 gttcaggtgg tggagtaaga cctggattcg aaggtggaca gatgccttta tacagacgta
     2101 ttcctaagag aggtttcaaa tgcagaaact ctaaggaaat cgttggtatc aacctttcag
     2161 ctttagaaag atttgaagac ggagcagaag tatctgtaga agctttaatc gaagcaggaa
     2221 tcgttaaaaa tcctagagat ggagttaaaa tcttaggaaa cggtgaatta acaaagaaat
     2281 taaatgtaaa agcaaatgca tttagtgcta gtgctaaaga gaagatcgaa gcacttggag
     2341 gaaaagctga ggtgatctag tatgtcaaac gctgttgtca gcgcttttaa aaacaagcaa
     2401 ttaagaaaga agctattatt tacgacacta attcttattg ttgtacgttt tggatcacaa
     2461 ctaccaattc cggaaatcga tagtgcacag atcagtgcct atttaaagag tacattaggc
     2521 gattcgttca gcctattgaa ctcttttacc ggaggctcct tcatgcagat gtcagtattt
     2581 gcattaagtg taactccgta tatcacatct tccatcatca tgcagctgat gacgatcgtt
     2641 attccagctc ttgaagagat gcagaaagat ggagaagacg gaagaaaaag aatggcaaag
     2701 attacaagat atgtaacggt tgtacttgcg atcatcgaag gcgcaggact tgcgatcgga
     2761 tttgccaatc aaggtgcatt aggtaccgat tatacaacgt ttaccattgt tacaatgatc
     2821 attgctttaa ccgcaggtgc agttctggtt atgtggcttg gagaacgtat tacagagagt
     2881 ggaattggaa atggtatttc cattatcctg ctcgtaaaca ttgtatccgg aatgccggga
     2941 gattttactt ctctttataa tcagtttatg aagggaaaac agatcgggcc agctctgatc
     3001 gcaggatgtg tgatcgtagg agttgttctg gcagttgtcg tattcgttat cgttttatca
     3061 gatgcggaaa gacatatccc agtacagtat tctaagaaga tgcagggaag aaaacttgtt
     3121 ggtggacagc agagtaagat tccgctgaaa gttaacacag caggtgtaat cccaattatc
     3181 tttgcatcat ccattatgca gttcccaatc atgctgcaga atgtcttaaa atacgagaat
     3241 aacggattca ttggaaaagc attaacaagt ttgaactcat ctacctggtt tgatgcatcc
     3301 catccaaaaa gatcgatcgg tttattgatc tatattgttt tagtagtact gtttgcttat
     3361 ttctatacat cgattacatt taatccatta gagatttcaa acaatatgaa gaaacagggt
     3421 ggatttatcc caggaatccg accgggaaaa cctacagtag attatttgaa taaaatatta
     3481 aaatatatta tatttatagg tgctgcagga ctaacgatcg tggcagttgt tccattcttc
     3541 tttaatggag tatttggagc aagcgtttca tttggaggaa catctatcat catcgtagta
     3601 ggtgttatcc ttgagacgat caagcagatc cagtcacagt tgttagtaca gaactattct
     3661 ggttttttga gtgagtaact ataagaaaag ctgtagtgac aactacagct ttttgtgctt
     3721 atatggaatg tttttatata agcacaaaag cccaatggca ttaaaaacga tggtatggaa
     3781 acaaattgta tggtaactaa aaataaaaga gagacaaaag gaggatttgt catgaaaatt
     3841 attatgttag gagctccagg tgctggaaaa ggtactcagg caaaacagat tgcacaggcg
     3901 tacgatattc cacacatctc aacaggtgat attttcagag caaatattaa agaaggaaca
     3961 gagcttggaa agaaagcaca ggaatacatg gacaaaggac tgttagttcc agacgaactt
     4021 gtatgcgatc ttgttgttga tcgtatccac aaagatgatt gtgaaaaagg gtatatttta
     4081 gatggatttc caagaacgat tccacaagca aatgcattag atgaagcact tgcaaaagac
     4141 ggtgagaaga ttgaatttgc aattgatatc gaagttccag atgaaaatat catcaacaga
     4201 atgtcaggaa gaagatcctg caaagactgt ggagcaatct tccatgttca gtataatcct
     4261 ccaaagaaag aaaactgctg cgacgtatgt ggtggagaat taattcttcg cgatgacgac
     4321 aaagcagaaa ctgtaaaaaa acgtctggat gtttatcatg aacagacagc tccactgatc
     4381 gcacactata aacaagcagg aagcttacac gagatcgatg gaacacagga tatcaaagtt
     4441 gtttttgaag aaattaaatc tattttagga taagaggcag accatggcag ttacgatcaa
     4501 atcaaaaaaa gaaatcgaac ttatgcgaga agccggtaaa atgttagaag aggttcacaa
     4561 caagttagct gattttgtaa aaccaggaat ttctacatta gagatcgacc agtttggaga
     4621 gaaagcgatc agagatcttg gatgtgtacc aaacttctta aactataatg gatatccggc
     4681 atccatctgt gtatctgtca atgacgaagt cgtacatggt attccaagca aagaccggat
     4741 tttacaggat ggagacattg taagtctgga tgcaggactg atccatgaag gatatcattc
     4801 cgatgcagca agaacacatg cggttggaaa tatttccgaa gaagcaaaga gactgatcga
     4861 agtaacaaag caaagtttct ttgaaggaat caaacttgca gttccaggaa atcatttgtt
     4921 tgacatatct gaagcaattc agaaatatgt agaaagtaat ggttattctg tagtcagaga
     4981 tttagtggga catggaattg gaacacatct tcatgaagat ccacagattc cgaacttcaa
     5041 acagagaaga aaaggaatga aattaagacc aggaatgacg cttgccattg aaccaatggt
     5101 aaatgaaggt agttatgatg ttgtctggct ggacgatgac tggacagttg ttactgaaga
     5161 tggaagtctg gctgcacatt atgaaaacac gatcgtcatc acagaagatg gttgtgaaat
     5221 attgactttg tctgaatagg aggaataaaa tggcaaaatc agaagcaatt gaagttgaag
     5281 gagtagtgtt agaaaagtta ccaaacgcta tgttcaaggt agaaatcgaa ggtggacacg
     5341 taattttagc tcacatcagt ggaaagctta gaatgaatta catcaagatt ttaccaggtg
     5401 acaaagtaac actggcatta tcaccatatg atttatcaaa aggaagaatt atttggagag
     5461 acaaataaat tatttggaaa gataaataaa tttagaaaaa gtcttgaaat attcagaata
     5521 tgatgatata atcatacccg aacgtttttt gaaaggagat attcaagtga aggttagagc
     5581 atctgtaaaa ccaatttgcg aaaaatgcaa ggttatcaag agaaaaggat ctgtcagagt
     5641 aatctgtgag aatccaaaac acaaacagag acagggataa ttacgacgtt cgataagaat
     5701 atcgcaataa tccttggttt gttgtgtatt cacctattta ctgcaagaac agtttagagg
     5761 tgtattttgt gatgtgtaga gatacactcg cataatttag gcggctgcag tatgagtaca
     5821 ggtacgctca tactgattat ttatagatag aaagataatt tgaggaagag acatatatat
     5881 catttcaccg tactgcggat atatggattg cccttaatag acaagtaatt atttattttg
     5941 gaggaaagaa aaacatggct cgtatttcag gtgttgattt accaagagaa aagcgtgtag
     6001 agattggtct tacttatatt tatggaatcg gtctttcaag cgcaaaacgt attttggcag
     6061 aggcaaatgt tgatcctgat actcgtgtac gcgacttaac tgatgatgaa gttaaaagaa
     6121 tcagtgcagt tatcaacgat acacaggttg tagaaggtga tttaagaaga gaaatcgctc
     6181 ttaacattaa gagattaaaa gaaattggat gctacagagg aaaacgtcat agacagggac
     6241 ttccttgccg tggacagaaa acaaaaacta acgcaagaac acgtaaaggt cctaagaaaa
     6301 cagtagctaa caagaagaaa taataaacac taaatttgta gtgttatgac acacccggct
     6361 gtatggacag tcaggaatag cataagattt gcagtatagg caatcttttt ggaattgaaa
     6421 tgaagaaaga aaggtatacg taattatggc taaagttaca aagaaaagtg caaaaaaacg
     6481 tgtaaagaag aatgtacagc acggacaggc acatattcag tcatctttta ataatacaat
     6541 cgttacatta acagatgcac agggaaatgc tctttcttgg gcaagtgcag gtggattagg
     6601 attcagagga tccaaaaaat ctacaccata tgcagctcag atggcagctg aaactgcaac
     6661 aaaagcagca ttagtgcatg gattaaaatc agtagatgta atggttaaag gtccaggatc
     6721 aggaagagaa gcagcaatcc gtgctttatc agcagcagga ttagaagtta caagcatcag
     6781 cgatgtaaca cctgttccac ataatggatg tcgcccacca aaacgtagaa gagtctaatt
     6841 taggaggata attaaagatg gcagtagata gaactccagt cttaaagaga tgtagatcct
     6901 taggtttaga tcctgtcttt ttaggaattg ataaaaaatc aaacagaagt ttaaatagag
     6961 caaacagaaa gaaaagtgaa tacggacttc agctttgcga aaaacagaaa gctaaattca
     7021 tttatggtgt attagaaaaa cctttccgta actactatgc aaaagcagaa agaatgaaag
     7081 gtcagactgg tacaaacctg atgacattat tagagcttcg tttagataac gtattattcc
     7141 gtttaggata cggaagaaca agaaaagaat gcagacagat cgttgaccat aaacatgtat
     7201 tagttaatgg aaaacaggtt aacattcctt cttaccaggt taaagctggg gacgttatcg
     7261 aaatcaaaga aaaacataga acatctccaa gatacaaaga gatcttagaa gtaacagaag
     7321 gaagaatgat tccttcatgg ttagaagcag atcatgataa ctttacagga actgtaaaag
     7381 aacttccatc aagagatgag atcgacgtac cagttaacga aacattaatc gtcgagttat
     7441 attctaaata ataaccctca agttaaacct aagccaaaag gagggcacgt atgtttgaat
     7501 ttgaaaaacc aaatattgag attaatgaga tttcagaaga taaccgtttt ggacgctttg
     7561 ttgtggaacc tttggaaaga ggttatggta caactttagg taattcctta agacgtatca
     7621 tgctttcttc tctgccaggt gcagcagtaa gttcagtaaa gatcaaaggt gttctgcatg
     7681 agttcagttc catcccaggg gttaaagaag atgtacctga gatcatcatg aatatcaaat
     7741 ctctggcaat taaaaacaac agctcatccg ctgaaacaaa gcaggcttat attgagtttt
     7801 caggaaaggg tgtagtaact gccggagata ttaaagcgga cagtgatatc gaaatcttaa
     7861 atccagagct tgtgatcgca acattaagtg atgacagcag caaactggat atggagctta
     7921 caatcacaaa cggacgcgga tatgtaggcg cagacaaagc agacaaagaa ggaaaagcaa
     7981 tcgatgagat cgcagttgat gcgatttaca caccagttga gcgtgtaaac ttaaaggttg
     8041 agaatactcg tgtaggtcag atcacagatt atgacaaatt aacattagat gtattcacaa
     8101 acggaacact aacaccagat gagtctgtaa gtttagcagc gaaagtatta tgcgagcact
     8161 taaacttatt tgtagatctt tctgagaatg ccaagacagc agaagttatg gtagaaaaag
     8221 aagatgacga gaaagaaaaa gttcttgaaa tgagcattga tgaattagaa ttatctgttc
     8281 gttcatacaa ttgcttaaag agagctggca tcaatacagt agaagaatta actaataaga
     8341 cttcagaaga tatgatgaaa gttcgtaact taggacgtaa atcattagaa gaagttttag
     8401 ctaagttaag tgaattagga ttatccctaa gttcaggaga agaatagaaa ccagacccgc
     8461 ctaggctgaa ggcataagta agcaaggcga aaaaacacag gaggtcatta taatggcaaa
     8521 atacagaaaa ttaggaagaa cagcttccca gagaaaagca ctaatccgtg cagaagtaac
     8581 aaatctgtta catcatggaa aaatcgttac aactgaagca aaagcaaaag aaatccgcaa
     8641 ggtagctgag aaactgatcg ctttagctgt aaaagaaaaa gacaactttg aaacagttac
     8701 agttgacgct aaagttgcta agaaagatga aaacggaaaa cgcgtgaaag aagttgtaga
     8761 tggtaagaaa gttactgttt atgaaacagt tcagaaagaa atcaaaaaag atttaccatc
     8821 ccgcttacat gcaagaagac agatgttaaa ggtattatat cctgtaacag aagttcctac
     8881 agaagcagct ggtaagaaag ctaatacaaa gaaagttgat ttaacagcta agttattcga
     8941 cgagtatgca ccaaaatatg ctgaacgtaa cggtggttac acaagaatcg ttaagattgc
     9001 tcagcgtaaa ggtgatgcag cattaaaagt agtacttgaa ttagtttaat caacagttca
     9061 atactgacta tattatttaa attccaaatc gacaacaaca aagaggtctt attccggaac
     9121 aactgggata agacttttct ttttttatgg gaaatagtat actatcctta ttacggatca
     9181 gataggagaa tattatgtcc atattgaaaa taaaagactt agttcacaaa tatgtgacct
     9241 atggagacac agaagaagac aagcaagaga ttgcagcaat cgatcatatt gatatcaatg
     9301 ttgagaaagg tgaatttgta gcaatccttg gaaagaatgg ttctgggaaa tcatcgcttg
     9361 cgaagcatat caatggactt ttgatgccga cagaaggcac agtatatatc aaaggaatgg
     9421 atacaaaaga tgaaagtacc ctcttaaaag tcagacagag tgcaggaatc gtatttcaga
     9481 atccagataa ccagatcgta ggaacaacgg tagaagaaga cacagccttc ggtccagaaa
     9541 acctcggaat agcttccaaa gatatctgga caaggatcac agcggctctt acaggtgtgg
     9601 gaatgatcgc atataaagaa gcaagtccca atcatctttc tggcggacag aaacagagag
     9661 tagcgatcgc agggatcatg gcaatggaac cagaatgtat cgtattagac gaaccaacag
     9721 ccatgctaga tccagaaggc cgcagacatg ttttatctct tgtgaaagca ttgaataaag
     9781 aaaagaatat tacgatcctt atggtaacac atcatatgga agaagtaatc ttagcagatc
     9841 gtatcatcgt gatggatcaa ggaaagattg tcatggatgg aaaaccaaaa gagatctttt
     9901 caagagttga tgagatgaaa acattaggtc tggaagttcc gtacgcaaca gaacttgcat
     9961 gggaactaaa aaaagaaggt gttaacttgt cagatacgat aacaacaaag gaagagctgg
    10021 tggaggaatt atgtcaattc gtttagaaca tgtaagttac gcatatgaag ataattctag
    10081 caagaagaat tacgcattaa aagatataaa cttagaaata aaaaaagaag aattcattgc
    10141 agtgatcgga cacacaggtt ctggaaaatc aacattgatc cagcatctaa atggattgat
    10201 caaaccaacg tctggaacaa tatatttcga tgatgaagat atatattcag aaggataccc
    10261 attacgagaa ttacgtggaa aagtcggtat atgtttccag tatccagaac atcagctatt
    10321 tgagacaacg attttagatg atgtatgttt tggaccaatg aactttggaa agacaaaaga
    10381 agaagcaaca gagattgctg tcaaatgttt aaaagatgtt ggattaccag agaaattata
    10441 tcaaaaatcg ccatttgaat tatctggcgg acagaagcga agagtagcga tcgcagggat
    10501 tcttgcaatg gagccagaat atttcatttt ggatgaacca acagcaggac ttgatccggt
    10561 cggaaaagat cagatcttaa atctgttaaa gaaacttcat gaagaaaaga agattacgat
    10621 cattctggta tcacacagta tggaagatgt tgcagattat gcacagtgtg tgatcgttat
    10681 gaatggtgga caggtcatgt atgaaggaga taaaaaagaa gtattctctc acaagaaaga
    10741 actagaagaa gcaggacttg ctgtaccgtt ttatcgtcaa gtatgtgaag aactcgcaga
    10801 cagagggttc ccaatccaga gggatttatt gacattagaa gaaacaaaag aagcaatttt
    10861 agaaggacta aaaggactga gaggaaaata aaatgctcaa ggatataaca attggacaat
    10921 attataaaga agactctctg atccacagac tcgatccgag agtgaaatta tttgcagtct
    10981 tgatctacgt cattacatta tttatgcata aagcacctgc aacttatgtg atgtcactat
    11041 tatttttgat cataacgatc aagatttcaa aggtacctgt aaaatatatt ttaagaggat
    11101 taaaagcaat tgcagtgatc ttattattct ctgtaatgat caatatgtta tttattccag
    11161 gaaaaccagt gatccagttc tggatcatat caatttctgc agaagggatt aggacaacta
    11221 tttatctggg aagccgtctg atcatgctgg tattaggaac gtctttactg acatttacaa
    11281 caacaccaaa tgaactgaca gacggactag acaaatcact gggattttta aataaagtcg
    11341 gagtaccagt acatgagatt gctatgatga tgtcaatcgc actaagattt attccaatct
    11401 taacagaaga attagaaaag atcatgaaag cccagacagc cagaggaatc gactttgaat
    11461 ccggaggtct cttaaaacga gtaaaaagca tggttccaat tatagtacca ctgtttgtag
    11521 cagcaattcg aagagcgaat gaccttgcaa tggcaatgga atcccgttgt taccatggcg
    11581 gtgttggaag aactaaatta aaaccattaa aatacgagaa aagagataaa atagcgtata
    11641 tcgtcctgtt tgtattttta gcagtgatga tctatttatc ctttttcagt ccatggaggt
    11701 aagatacgat gaaacgagta aaattaatcg tagcctatga tggaactggt tattgtggat
    11761 ggcaggtaca gccaaacgag attacagtcg aaggtgtctt aaataagcat atatcagaac
    11821 tgacaggaga agacatcaaa gtgatcggag ccagcagaac ggattcagga gtccacgcat
    11881 taggaaatgt agctgtattc gacacagaaa gccgtattcc gggagagaag atggcaaaag
    11941 cattaaatgc aagactacca gaagatatca ttattcagga ctccaaagaa gtgccattag
    12001 actaccatcc aaggtttcag gatacaagaa agacatatga atataccttt tacaatgcaa
    12061 gatacgacaa tcccgtgaca agcagacatc atcattttgt atatgtacca cttgatgtgg
    12121 agaaaatgaa agaagcagct tcttatttct taggggaaca ctgttttatc agtatgtgca
    12181 gcagtaaagc acaggttaca tcctatgtca gagagattta cgaatgtgat gtgacacaaa
    12241 atggcagata tatcacaatg agagtcacag gaagtggttt tctctataat atggtacgtt
    12301 taatggcagg aacgctttta gaggtcggaa gaggaaaaca agaaccacaa tgggtcaaag
    12361 aaatcttagc atccaaagaa agagttacac caggaccaaa acttccagca aaaggcttga
    12421 cattaattca gatcgaatat ccggaggacc gcgagaatgc agaatagctt tatcatattt
    12481 cagaaattgt tagtattgtt tggttttatg ctgatcggat acctaagtta taagaaaaaa
    12541 tggatcagtg atgatacaag ttcacagata tcaggactga tcgtaaatat atttaatcct
    12601 gcattgatca tatctggcgt gatcggttcc gttggaaatg gaaactggaa tcttgtgatc
    12661 atggacctga tccttgcagt gatcttattt gtggtattga tcctgatcag tccggcattt
    12721 gttcgtattc taagtgtaaa aaaagatgaa agaaatatct atgcagtgat gctgatcttt
    12781 tcgaatcttg gatttatggg gattccgatc attgaagaat tatacggtag aggagctatt
    12841 ttttatgtag cactgtatac actggtatat aatattctct tttacaccta tggaatttat
    12901 ctttttgaga aagaacgtgc catgcagaca ggacaaaaag ctaagatcat attccactgg
    12961 aaaaagatga tcaatccagg aatggtcgca tgtctggctg cgttattgat ttttgcattt
    13021 cagatcgacg caccagcacc tgcggtaagt tttgtacaat acttaggaaa tgcagcaatc
    13081 ccactctcca tgatcattac cggggtatct ttagcgaaga tgccgctgat cgaagttttc
    13141 aaagacatca agatgtatca gtttacattt ttaaagatgc tggtgatccc aatgattgca
    13201 gcatttgcga tccggctgtt tcatttagat cctgttctct cggggatcat ggtattaatg
    13261 tttggaatgc caaatggaag catggcagtg atgatggcaa tcgattatgg attagacagt
    13321 tccatttgca gcagaggaat cgtactgaca acactgttat cgatcataac gttgccgatt
    13381 gtagcatatc ttatttgaca ttgagattta tcaacaatgt tttaacatat acggacataa
    13441 tatacccata tattgtggat aaaattttga aatcaagacc atcttgtgga taacatcaaa
    13501 aatttatcaa aaacaacaag aaaaaatctt gacacacccg gctgcgtatt atataataag
    13561 aaactgtgac atgcgagaga atactacccc aatgccccgg tgggagtatt taatatagac
    13621 acatatgatt gatagaaatt ttaaggagga acaccatgaa aagttttatg gccagcccat
    13681 caacaattga aagagaatgg tatgtagttg atgctacagg acatacatta ggacgtttag
    13741 catctgagat cgcaacaatc ttaagaggta aaaacaaacc aacttataca ccacatatcg
    13801 atacaggaga taacgtgatc gttatcaacg ctgagaagat taaagtaact ggtaagaaat
    13861 tagaccagaa gatctactat catcattcag actatgttgg tggaatgaaa gaacagacat
    13921 taaaagaaaa attagctaaa aaacctgaag acgtaatcta tcttgcagtt aaaggaatgc
    13981 ttccaaaagg acctttagga agacagatga ttaagaaact tcacgtatat gcaggtgcag
    14041 agcacaacca tgcagctcag cagccaaaag cattagagat caagtattaa tttttgaagg
    14101 aggaagaagc agtggctaca gcaaaatatt acggaactgg tagaagaaaa cacagtgttg
    14161 ccagagtata tttagtacct ggaacaggaa aaatcacaat caacaaaaga gatattgatg
    14221 aatatcttgg attagaaaca ttaaaagtag ttgtacgtca gccattagtt gcaacagata
    14281 caattgacaa atatgatgta ttagtaaacg ttcatggtgg tggatttaca ggacaggctg
    14341 gagcaatccg tcatggtatt tccagagctt tattacaggt tgatggagaa ttccgttcaa
    14401 cattaaagaa agctggattc ttaacaagag acccaagaat gaaagaaaga aagaaaccag
    14461 gtcttcgcaa agcacgtcgt gcaccacagt tctcaaaacg ttaatcatac gttacggaag
    14521 atacgaagaa tatacagagc ctttcaatgg agaaatctat tggaaggctt tttctaatga
    14581 tttggtttgg aaaacttaat ttcttagaaa tatagcggca aaagatatgc taagctatat
    14641 ttcaaaagca agaagtggtt ctggtatatt taatgcagaa aaaatgtcag tttatgtacc
    14701 actaggaaca caacaaatgt tatctgatta tggatatcat attgtaacag aataagatag
    14761 gaggaaggta aaatgaaatt aaaaaatcta acaaaaaaat taacaacttt agcaatcaca
    14821 gccttaatgg caacaacatt ggtttcaact caaaatgtat tagctgcatc aaaaaaagca
    14881 cagaagaagg cgaacaaagc ctatgcacaa gcaatcaaga aaaacaaagt agccaaattt
    14941 acaagctatt cattaattga agtaacagga ggcggaacaa aagaactggt cactgtgaat
    15001 ttaaatagta atgcagaagg cgaagctaga aatttaaata tttatacata caataaagga
    15061 aaagtaaaga aaatttggtc aacatcggat caaataattg catataataa aaaacagaaa
    15121 acaattgcag gcttacagta ttcagtatta agtgaaaatg gttctgaaag ggaaaaatat
    15181 tatatttata agtatgatgg aaagaaaatt gtaaaaaaag catcatattt ctt
//
LOCUS       DAOMUE010000018        15188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_817_length_15188_cov_3.191898, whole genome shotgun sequence.
ACCESSION   DAOMUE010000018 DAOMUE010000000
VERSION     DAOMUE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 15188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15188
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_817_length_15188_cov_3.191898"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PLC71_01880"
     CDS             <1..593
                     /locus_tag="PLC71_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM01428.1"
                     /translation="MGKKVEIKNMEFKSLIGAMESDSINAIIAGMTKTEKREKAVDFS
                     DSYYTTNQAIILKKDSKITKLAQLDGKKIGVQEGTTGDLISSGDKFGDGKKAKVLVKG
                     AKVKRFKKGVDAVMDLKNGSVDAVVIDELTANEFVKKNAKKLKIVIDSAGSEYYCIAL
                     TKGNTAYKEEVNKQIKAIKEDGTYDKLKAKYIDSKN"
     gene            619..1296
                     /locus_tag="PLC71_01885"
     CDS             619..1296
                     /locus_tag="PLC71_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM01429.1"
                     /translation="MLDSFYTAFIEEQRYLLYLDGLKMTILVSVIAIIIGVVLGTLLA
                     LMRLTAEQKGKSTLLSKIAYVYIDIIRGTPTLTQLMIMYFVILKGQSGLLVGSLTFGL
                     NSAAYVAEIIRAGIQAVDQGQMEGGRSLGLSYVQTMKDIILPQAIKNILPALGNEFIV
                     LVKETSILGYVAIVDLTKVSDFVISRIFEALAPLLGTALIYYVIVKFLTLGLNALERR
                     LRQSDRR"
     gene            1283..2005
                     /locus_tag="PLC71_01890"
     CDS             1283..2005
                     /locus_tag="PLC71_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM01430.1"
                     /translation="MIDVKNLHKYFGSLEVLKGIDCHIDKGECVCVIGPSGSGKSTFL
                     RCLNLLETPTKGDIVIDDMHLTEKDFDVDAMRKRVGMVFQHFNLFPHLTILENVTLAP
                     IRHKMMTEEQAKEKAMELLNRVGVGDKADNYPAQLSGGQKQRVAIARSLALSPEVMLF
                     DEPTSALDPEMVGEVLEVMKQLAQEGMTMVVVTHEMGFAREVANRVFFMDGGGILEEG
                     TPEQIFDHPQEERTKAFLSKVL"
     gene            2217..3521
                     /locus_tag="PLC71_01895"
     CDS             2217..3521
                     /locus_tag="PLC71_01895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM01431.1"
                     /translation="MNQNVKITRQQAEEEAKMLEKVFDVVRFLEGDFFEQMQDDPRIG
                     VKIGMCQCYDFWKKNKPCENCTSMKAYAEKKQKTKLEFLDADVFLVISRYLEIDDEAC
                     VMELVKHLENDTLIDTDGRDKLVGKLKGYQDKLYIDPVTGVYNRRYFEDEIRNMQNSA
                     GVAMIDLDDFKLYNDIYGHDMGDQVLCIVADVIKNCIRKTDKLIRYGGDEFLLILSDM
                     VRGTLRGKLLQIQEAIENATIPNCSRLKLTASIGGVISEDGQIDEAIAKADQLMYKAK
                     DHKAQVITECDKTIFKKEKIQNKPKILIVDNSEFNRAILKEILEETYEIIEADGGNEA
                     LHKIDEYGMKISLVLLDIIMPEKDGFEVLKYMEEERLISDIPVIIISSEDSANYIRRA
                     YEMGVSDYINRPFDANIVYQRVSNTVKLYAKQRRLMAMVTRV"
     gene            3557..4381
                     /locus_tag="PLC71_01900"
     CDS             3557..4381
                     /locus_tag="PLC71_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM01432.1"
                     /translation="MQLKKLMIEVMVFFVLGCFLCACGKTKDQTRKTTKDANKEIMIG
                     GDNYPPFNYTDENGNLAGIDVEFAREAFHRMGYKPVFVNIDWENKKNLVEQGKIDCIW
                     ACFSVTGRENDYHWAGPYMLSRQVIAVNDSSKINKLSDLKNKIIAVQSTTKPEELFLK
                     QTDPKIPKVKQVYSMENRELIYTSLSRGYVDAIAAHEISIRQYMSDFEAKYRILDEDI
                     LETGIGVAFAKNDKRGIEKELSKTLKAMVKDGSAKKILQKYVPDAEKYLEVNSLER"
     gene            4371..6527
                     /locus_tag="PLC71_01905"
     CDS             4371..6527
                     /locus_tag="PLC71_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM01433.1"
                     /translation="MRDKADKRLWTLSIVGCVIVVLTVLFVSVKIETNNTKNRIHSTM
                     EYIKDQYFMYNSFNEAVEARSLVRMVEKVQQTARNFKTDKQALEKEYLKQYAREQRLT
                     GIIVLNDKNKQIAQYHTGPRFKKVLGYVMEKETLRDVKNYPQKSYMARIELKNGGYID
                     LASYGRKDAKGTVIGYQYVKTEYAKNNNLTIQNVLAGYKRENDGTIVITNGNDIIASN
                     DEKLIDTKVQEDSIINEIRQSVKVGQFAIIRNGIKQYFTSLDKGRNYYIYVYAEDRNL
                     VKTLTSNIGIAVILYGIFVAILMMIKARSNQQYERIQKQREEEYKKELEKSAKEAKKA
                     NIAKTEFLQRMSHDIRTPINGIRGMVEVGDYYKSDIEKQSECRKKIWEASGFLLELIN
                     EVLDMGKLESEEVILERRSFNFFQLFQEIRMVIEKQARERGINIVVHKYNVIHEDLIG
                     SPVHVKRVVMNILTNAIKYNKNNGKICIEFNEIQKNYNTIIIRFKCEDTGIGMSESFQ
                     KTIYEPFAQEKAGARTVYGGTGLGMPITKSLVEKMGGTISFESEQGVGTTFYIELPFQ
                     IDHNIKHEELKTKEIKKASIKGVNVLLAEDNELNMEIAEFVLESAGANVIKAFNGKEA
                     LEIFKESKLGEIDIILMDVMMPVMDGLEAARYIRWSNKENARDIPIIAMTANAFTEDR
                     RRVLEAGMNEHLAKPLESEVLIKTIANYCGKVDHFK"
     gene            6671..6982
                     /locus_tag="PLC71_01910"
     CDS             6671..6982
                     /locus_tag="PLC71_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01434.1"
                     /translation="MERIAEKLSEIEKTARAIVDNAQEQKHQMEMQMQKKRDAFDADM
                     EKETNEKILKIQSDLATNMEKLLKKQEEQNNNEIESLKQDFKEHHSKYAKQILERVIK
                     V"
     gene            6995..8062
                     /locus_tag="PLC71_01915"
     CDS             6995..8062
                     /locus_tag="PLC71_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRM01435.1"
                     /translation="MRDVMRYSGIVTKVAAMRAKLLKPQDYEQLASMDTVTDIIEYLK
                     QTKSYGKFITQMDESLYHRGNIEKVLIQSLYDDYTRLYRFADMQQKDFLKIFMKRYEV
                     DLVRYCLRIVFNHSNVPFDLNYKKPFFDKYSKIRIDQLVTAKNIDHLVDYLKNTEYYA
                     PLSRIRQSGASTLADYELALDLYYFSMMWKERKGNWDKKDKEMLTKELGAKIDLLNLQ
                     WIYRAKKYYHMLAPDIYTLLIPIQHKLSNQEFKDLVEAPSVEEFKRRLNETYYGKKYE
                     FGEQVQIESIVNECVEHILTIAYRNHPYSLASIQQYLFLKEEEIYKITTALECIRYGL
                     SQRETLQYLVQKQRRQGGNAS"
     gene            8059..10008
                     /locus_tag="PLC71_01920"
     CDS             8059..10008
                     /locus_tag="PLC71_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRM01436.1"
                     /translation="MIVKMKFLSISGPRTDIDRVSASYLSKYEMQLENAITELKTTDN
                     LMPFMDVNPYKEPLSKAEQYVLMLAQPKGEMDTSLTADEILTMIRDLNHDYLDLKARE
                     DRLKKQKEELLEKLHELEPFQPLELNLKEVYGYQYMKVRFGRVNVEYYQKLEKYLFDD
                     LNAIFLEGIRNENYVYGCYFAAHKEMGKIDSTFKSLHFENIALPDDYEGMPAEICRNL
                     RNQIDEIEKEIENVQKQMKDFLSVKAKKIRGARYKLEELSNNFDVRKMAACMENEEQE
                     DYYILCGWMSEKDTQAFIEETKDDDRITIIVEEGREKFFGDPPTKLQNPKFFKPFEMF
                     IRMYGLPSHDEMDPTIFVALTYTFIFGAMFGDVGQGLCLFIGGGLLYKIKKWNLAGII
                     SVAGIFSTFFGFMFGSFFGFEGTIIKPMWLSPMHAMMKLPFVGQLNTVFIVAVAFGMA
                     LILLAMVFQIINAKKRGDKENLFFSPNGVAGLVFYGFLVLTIVLYMTGHKTPGNIMMI
                     IFLGIPVIMFLLKEPLGQLVEGKKPKAEGGVGMFLVQGFFELFETMLSFFSNTISFVR
                     IGAFAVSHAAMMEVVLMLGGITDGAGNPNWIIIVLGNIIVCGLEGLVVGIQVLRLEYY
                     EMFSRFYTGSGREFHPYDNHAGKEN"
     gene            10021..10455
                     /locus_tag="PLC71_01925"
     CDS             10021..10455
                     /locus_tag="PLC71_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HRM01437.1"
                     /translation="MTLAIKLAIIAALILSIFIPFGYFLHGERTKKRYKRSIAFNAFF
                     FFGIIVIAGVMMFVTNPVQAAQAGSDAGMATGLGYLAAALSTGLSCVGGGIAVASAAS
                     AALGAISEDPSALGKSLIFVGLAEGVCLYGLIISFMIIGKLG"
     gene            10473..10781
                     /locus_tag="PLC71_01930"
     CDS             10473..10781
                     /locus_tag="PLC71_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRM01438.1"
                     /translation="MKMYLISDNLDTLTGMRLAGVDGIVVHERDELKETIEKAMSDPE
                     IGIVLLTEKFGREFPELIDEIKLERKMPLLIEIPDRHGTGRKKDFITSYVNEAIGLKL
                     "
     gene            10800..11396
                     /locus_tag="PLC71_01935"
     CDS             10800..11396
                     /locus_tag="PLC71_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HRM01439.1"
                     /translation="MTTLTVEEKISHIREAAMEEARARGNEIIDQHQKALEGVFKTHK
                     QEAVMQADTRIKTETASARQQLNTVTSKGQLKLRRQLSRVQNELKNKLFEEVREMAEE
                     YMKTEAYKELLVSYIAKAARFADGNPLTIYINSSDEDKKEFLEKRTGMTVTVSEEDFL
                     GGIRSIIPGRNILIDHSFSGALEKEYEEFTFKGGVTGE"
     gene            11389..13155
                     /locus_tag="PLC71_01940"
     CDS             11389..13155
                     /locus_tag="PLC71_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRM01440.1"
                     /translation="MSKTGTIYGINGPVIYLAGNTGFKMSEMVYVGKEKLVGEVISLD
                     KDRTTIQVYEETSGLKPGEIVEASGEAVSVTLAPGILDNIFDGIERPLERIAENAGAF
                     ITRGISVDSLDMEKLWDTKLVVNEGDVLHGGDIYAQVQETRAIVHKCMVPPDLTGTVT
                     FVASDGEHAIKDHMITLRLDDGTEKQLTMIQRWPIRVPRPVHDRIPACVPLVTGQRIL
                     DTMFPIAKGGTAAIPGGFGTGKTMTQHQIAKWSDADIIIYIGCGERGNEMTQVLEEFS
                     ELVDPKSGNPLMDRTTLIANTSNMPVAAREASIYTGLTLAEYYRDMGYDVAIMADSTS
                     RWAEALRELSGRLEEMPAEEGFPAYLASRLSAFYERAGMMHNLNGTDGSVTIIGAVSP
                     QGGDFSEPVTQNTKRFVRCFWGLDKSLAYARHFPAIHWLTSYSEYLTDLGGWYRDHVS
                     PNFVDYRNRLMAILNQESSLMEIVKLIGGDVLPDDQKLTLEIARVIRLGFLQQNAFHK
                     DDTCVSMEKQFKMMEVILYLYQKSRELVARGMPMSVLKAEGIFEKVIAIKYDVPNDNL
                     QLLDLYRKQIDDFYDAVLEKNA"
     gene            13171..14556
                     /locus_tag="PLC71_01945"
     CDS             13171..14556
                     /locus_tag="PLC71_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRM01441.1"
                     /translation="MGIEYLGLSSIKGPLVILEGVQDAFFDEIVEFTVDGKTKKIGRI
                     IELYEDKAVIQVFEGTENMSLDNTRTKLTGHPMEVSLAPDMLGRTFNGIGKPIDGLGP
                     LITDVKRDVNGLPLNPVRRKYPRNYIRTGISAIDGLTTLIRGQKLPIFSGNGLPHDQL
                     AAQIVKQASLGDGSDEPFAVVFGAMGVKHDVADFFQKTFEESGVADHVCMFLNLANDP
                     VVERLITPKVALTAAEYLAFDCNMHILVILTDMTSFAEAMREVSSSKGEIPSRKGYPG
                     YLYSELATLYERAGIIEGAEGSVTQLPILTMPNDDITHPIPDLTGYITEGQIVLDRNL
                     NGQSIYPPISVLPSLSRLMKDGIGEGYTREDHQDLANQLFSAYAKVGEARNLASVIGE
                     DELSPIDKKYLEFGKAFEERYIGQGPEENRSMIETLDLGWELLKILPREELDRIDTKI
                     IDKYLPKEQDS"
     gene            14570..>15188
                     /locus_tag="PLC71_01950"
     CDS             14570..>15188
                     /locus_tag="PLC71_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRM01442.1"
                     /translation="MDPREFPTKGNLIAAKNSLTLAKQGYDLMDKKRNILIRELMDLI
                     DEAKDIQEEIDTTFTRAYACLQRANIQHGISKVEELAYTVPIEDSIQIQTRSIMGTEI
                     PHVKYDAHENQLTYAMDGTFESIDVAREAFRQVKDLTIKLSMVENAAYRLATSIKKTQ
                     KRANALKNITIPTYTELVYKISNELEEKEREEFTRLKVIKRMKQKN"
BASE COUNT         5348 a         2268 c         3560 g         4012 t
ORIGIN      
        1 gtatgggtaa aaaagttgag atcaaaaaca tggaattcaa atctctgatc ggtgctatgg
       61 aatctgattc tatcaatgcg atcattgctg gaatgacaaa gacagagaaa cgtgaaaaag
      121 ctgtagattt ctctgacagc tactatacaa caaatcaggc gatcatctta aagaaggata
      181 gcaagatcac aaaattagct cagcttgatg gaaagaagat tggtgttcag gaaggaacaa
      241 caggagattt aatttcatct ggagataaat ttggagatgg taagaaagcg aaagtccttg
      301 tcaaaggtgc aaaggtaaaa cgttttaaga agggcgttga tgctgtcatg gatctgaaaa
      361 atggatcagt tgatgcagta gtcatcgatg aattaacagc aaatgaattt gtgaaaaaga
      421 atgcgaagaa gttaaaaata gtgatcgatt ctgcaggatc tgaatactat tgtatcgcac
      481 ttacaaaagg taatacagct tacaaagaag aggtcaataa acagattaaa gcaattaaag
      541 aagatggaac atatgataag ctgaaagcta aatatattga ttccaaaaac tagaaaatag
      601 gaaggtaagg tttcaaaaat gttagattca ttttataccg catttattga agaacaacgt
      661 tacctgctat acctcgatgg actaaagatg acaattcttg tgtcagtcat tgcgatcatc
      721 ataggtgtgg tacttggtac attgttagca ttaatgcgtt tgacagcaga gcagaaagga
      781 aagagtacac ttttatcaaa aatcgcatac gtgtacattg atatcattcg tggaacacca
      841 acgttgacac agttgatgat catgtatttc gttattttaa aaggtcaaag tggactgctt
      901 gtaggttcct taacctttgg tctaaacagt gcagcttatg ttgcggagat catccgtgca
      961 ggaattcagg cggttgatca gggacagatg gaaggtggac gttctcttgg attatcctat
     1021 gtacagacaa tgaaagatat tatcctgcct caggcgatta agaacattct tcctgccctt
     1081 ggaaatgaat ttatcgtact ggtaaaagaa acttctattt taggatatgt agcgatcgtc
     1141 gatctgacaa aggtatcaga tttcgtaatt tcaagaatct tcgaagcact ggcaccattg
     1201 cttggaacag cactgatcta ctatgtgatc gttaagttcc tgacattagg attaaacgca
     1261 ttagaaagga gactgagaca aagtgataga cgttaagaat ttacataaat atttcggatc
     1321 tctggaagtg ttaaaaggca ttgactgcca tattgacaaa ggagaatgcg tttgcgtgat
     1381 cggaccatcc ggatctggaa agagtacatt tttaagatgc ttaaacttat tagagactcc
     1441 gacaaaggga gatattgtca tcgatgatat gcatttgaca gagaaagact ttgatgtaga
     1501 tgcgatgaga aaacgagttg gaatggtatt tcagcatttt aacttattcc cacatctgac
     1561 aatccttgag aatgttacat tagctccgat ccgtcacaag atgatgacag aagagcaggc
     1621 aaaagaaaaa gcgatggaat tgttaaaccg tgtaggcgtt ggtgataaag ctgataatta
     1681 tccagcgcag ttatctggtg gacagaaaca gcgtgtagcg atcgcaagat cactggcatt
     1741 atcaccagaa gtcatgctgt ttgacgaacc aacttcagca cttgaccctg agatggttgg
     1801 agaagtatta gaagttatga aacagcttgc ccaagaaggt atgacaatgg ttgtcgtaac
     1861 tcatgagatg ggatttgcaa gagaagttgc taatcgtgta tttttcatgg atggtggagg
     1921 aatccttgaa gaaggaacac cagaacagat ttttgaccat ccacaggaag agcgaacaaa
     1981 ggcattttta agcaaagtat tataaaagat cataagaata agaaggaaga tacacatact
     2041 ttaatgttat atcgcccttc tttttgtata taaaaaaata aatttgatat taatttagga
     2101 aatctttgta aaattcaggt ttctcgatgg aaaacgacaa aatgatgtgc tatcataaaa
     2161 gggtatttaa gtgaattagg ggaatatatc aaataaaaat aggaagggga taagaagtga
     2221 accagaatgt aaagataacc agacaacagg cagaagaaga agctaaaatg ctggagaaag
     2281 tatttgatgt tgtcaggttt ttggaggggg atttttttga gcagatgcaa gatgatccac
     2341 gcatcggagt aaaaattgga atgtgtcagt gctatgattt ttggaagaag aataaaccat
     2401 gtgagaattg tacatcaatg aaagcatacg cggagaagaa acaaaaaaca aaactggaat
     2461 ttttggatgc agatgttttt ttggtgattt caagatattt ggaaatagat gatgaagcat
     2521 gtgtcatgga acttgttaag catcttgaga atgatacgtt gatcgatacc gatggaaggg
     2581 acaaacttgt tggcaagtta aaagggtatc aggataaatt atacatagat ccggtaacag
     2641 gcgtttacaa tcgaagatat tttgaagatg agattcgtaa tatgcagaat tcggcagggg
     2701 ttgcgatgat cgatctggat gattttaaat tgtataatga tatttatgga catgatatgg
     2761 gagatcaggt tctctgtatt gttgcggatg tgatcaagaa ttgtattcgc aagacagata
     2821 agttaatacg ttatggcgga gatgaatttt tattgatctt gtcggatatg gtaagaggta
     2881 ctttgcgtgg aaagctgctt cagattcaag aggctattga gaatgcaacg attccaaact
     2941 gctctagatt gaagcttaca gcaagtattg gaggagtgat atcggaagat gggcagatcg
     3001 atgaggcgat cgcgaaagca gaccagttga tgtataaggc aaaagatcat aaggcacagg
     3061 tcataacaga atgtgataaa acgatattta aaaaggaaaa aattcagaat aaacctaaaa
     3121 ttttgatcgt tgataattca gaatttaacc gtgcgatctt gaaagagatt ttagaggaaa
     3181 cctacgaaat catagaagct gatggtggaa atgaagcatt gcataagatc gatgaatatg
     3241 gaatgaaaat ttcgcttgta ctattagata tcattatgcc agaaaaggat ggttttgaag
     3301 ttttgaaata tatggaagaa gagcgtttaa tctcagatat tccagttatt ataatctcaa
     3361 gtgaggattc tgcgaattat atcagaagag cttatgagat gggagtttct gattatatta
     3421 accgcccatt tgatgccaat attgtctatc agagagtatc taatacagtg aaattatatg
     3481 caaaacaaag aagattgatg gcaatggtta caagagttta ggacattatt tacaaacaga
     3541 tcatacgagg taaactatgc agttaaagaa gcttatgatt gaagtcatgg tctttttcgt
     3601 attaggctgc tttctttgtg catgtggaaa gacaaaagat cagactagaa aaacaacaaa
     3661 agatgcgaac aaagagatta tgatcggtgg agacaattat ccgccattta actataccga
     3721 tgagaatggc aatctggcag gaatcgatgt agaatttgcc agagaagcct ttcatcgtat
     3781 gggttataag ccggtctttg taaatatcga ttgggagaat aagaaaaatc ttgtggaaca
     3841 aggaaaaata gattgtatat gggcgtgctt ttcagtgaca ggaagagaaa atgattatca
     3901 ctgggcaggt ccttatatgc tgagccgtca ggtgatcgca gtgaacgaca gcagcaagat
     3961 caataagttg tctgatctta agaataaaat cattgcggtt cagtctacaa ccaaaccaga
     4021 ggaattattt ctaaaacaaa cagatcccaa aataccaaaa gtaaaacaag tctacagtat
     4081 ggaaaacaga gaattgatct atacttctct gagcagagga tatgtagacg ctattgcagc
     4141 acatgagatc tcgatcagac agtatatgtc tgattttgaa gcaaaataca gaattcttga
     4201 tgaagatatt cttgagactg gaattggtgt tgcgtttgca aaaaatgata aaagaggaat
     4261 agaaaaagaa ctgtcaaaga cattaaaagc aatggtcaaa gatggaagtg ccaaaaaaat
     4321 tctacaaaaa tatgttcctg atgcagaaaa atatctggag gtgaacagcc ttgagagata
     4381 aagcggataa aaggttatgg acattgagta ttgttggctg tgtgatcgtt gtcttgacag
     4441 ttctttttgt gtccgtaaaa atagaaacaa acaataccaa aaacaggatt cattcgacga
     4501 tggaatacat caaggatcaa tatttcatgt ataacagttt taatgaagca gttgaagcaa
     4561 gaagtcttgt cagaatggtt gaaaaagtcc agcagacggc gagaaatttc aaaacagata
     4621 aacaggcatt ggaaaaagaa tatttaaaac agtatgcaag ggaacaaaga cttacgggga
     4681 ttattgtatt aaatgataag aataaacaaa ttgcacaata tcatactgga ccaagattta
     4741 aaaaagtttt gggttatgtc atggagaaag aaacacttcg tgatgtaaag aattatccac
     4801 aaaaatcata tatggcaaga attgagctga aaaatggagg ctatattgat ctggcatcgt
     4861 atggaagaaa ggatgcaaaa ggaacggtta taggatatca gtatgtaaaa accgaatatg
     4921 cgaaaaacaa taatcttaca atccaaaacg tgcttgctgg atataagcgg gaaaatgatg
     4981 gaacgatcgt aatcacaaat ggaaatgata ttatagcatc aaatgatgaa aaattaattg
     5041 atacaaaggt acaggaagat tctattatta atgagatccg acaaagtgta aaagtaggac
     5101 agtttgcaat cattcgaaac ggtatcaaac aatattttac aagtcttgat aagggacgaa
     5161 attattatat ttacgtgtat gcagaagaca ggaatcttgt aaaaacattg acgagtaaca
     5221 ttggaatagc ggttatttta tatggaattt ttgtggcaat cttaatgatg atcaaagcaa
     5281 gatccaatca acaatatgaa cgcattcaga aacaacgaga agaagagtat aaaaaagaat
     5341 tagaaaaatc agcaaaagaa gcaaagaaag ccaatattgc caaaacagaa tttctgcaaa
     5401 gaatgagcca tgatatccgg acaccgatca atggaatccg gggaatggta gaagttggag
     5461 attattataa aagtgatata gaaaaacaat cagaatgccg caaaaaaata tgggaagcat
     5521 caggcttctt actagaattg atcaatgaag ttttggatat gggaaaattg gaatcggaag
     5581 aagtgatcct ggagagaaga agttttaatt tctttcaatt gtttcaagag attcgaatgg
     5641 ttattgaaaa acaggctaga gagagaggaa ttaatatagt tgtacataaa tataatgtga
     5701 ttcatgaaga tttgatcgga agtccagtgc atgtaaaaag agttgtgatg aatattttaa
     5761 caaacgcgat caaatataac aaaaataatg gaaagatctg catagaattt aatgaaattc
     5821 agaaaaatta taatactatt attattcgat ttaaatgtga agacactgga attggaatga
     5881 gtgaatcatt ccaaaagacg atctatgagc catttgcaca ggaaaaagca ggggcacgaa
     5941 cagtttatgg tggaacggga cttgggatgc cgattacaaa aagtcttgta gagaaaatgg
     6001 gaggaacaat ctcatttgag agtgagcaag gtgtgggaac gacgttttat attgagcttc
     6061 catttcagat cgatcataat ataaagcacg aagaacttaa gacaaaagag ataaaaaaag
     6121 catcgatcaa gggtgtcaat gtattgcttg ccgaagataa tgaattgaat atggaaattg
     6181 cagaatttgt tctggagagt gccggggcga atgtaattaa ggcatttaat ggaaaagaag
     6241 cattggaaat tttcaaagaa tcgaaactag gagaaattga tattatctta atggatgtga
     6301 tgatgccggt gatggacgga ttagaagcag cgagatatat aagatggagc aataaagaaa
     6361 atgccagaga tattccgatc attgcgatga cagcgaatgc atttacggaa gatcgtagga
     6421 gagttttgga agcgggaatg aatgagcatt tggcgaaacc gttggaatct gaggtgttga
     6481 tcaagacgat cgcgaattat tgtgggaagg tcgatcattt taaataataa tacaacagat
     6541 tgtaaaacat tgcatgaaaa ctgtcaaaaa attgtgaata attgctaaaa acatgcaaaa
     6601 aaacagataa aatactagca aaaataaata gaaaggacta caatggttgg tatgaaagga
     6661 ggttttactg atggaaagaa tagcagagaa actttcagag atcgaaaaga cagcgcgtgc
     6721 gatcgtagat aatgcgcagg aacaaaagca tcagatggaa atgcagatgc agaaaaagag
     6781 ggatgcattt gatgcagata tggaaaagga gacgaatgaa aagatattaa agattcagtc
     6841 ggatcttgca acaaacatgg aaaaacttct aaagaaacaa gaagaacaga ataacaatga
     6901 aatagaatct ttaaaacaag acttcaaaga acatcacagc aaatatgcaa aacagatatt
     6961 agagcgggtg atcaaagtat aggaggcgat agcattgaga gatgtgatgc gctacagcgg
     7021 aattgtgacg aaagtcgcag caatgcgtgc aaagctttta aaaccacagg attatgaaca
     7081 gctggcttct atggatacag tgacagatat cattgaatat ctgaaacaga caaaatcgta
     7141 cggaaaattt atcacacaga tggatgaatc actgtatcac agaggtaata tcgaaaaagt
     7201 cctgatccag tcattatatg atgattacac aagactttat cgtttcgcag atatgcagca
     7261 aaaagatttt ttaaagatct ttatgaagcg gtatgaagta gatttggttc gatattgctt
     7321 aagaattgta tttaatcaca gtaatgtacc atttgatctg aattacaaaa agccattttt
     7381 tgataaatat tcaaagatac ggattgacca gctagtgacg gcaaagaata ttgatcatct
     7441 ggtagattac ctgaagaata cagaatatta cgcaccactg tcaagaatcc gacagtcagg
     7501 ggcatcgaca ttagcggatt atgaactggc attggattta tattattttt ctatgatgtg
     7561 gaaagaacgc aagggaaatt gggataaaaa agataaggaa atgctgacta aagaattagg
     7621 agcaaagatc gatctattaa atcttcaatg gatttaccga gcaaagaaat attaccatat
     7681 gctcgcacca gatatttaca cattgttaat tccgattcaa cataaacttt cgaatcagga
     7741 atttaaagat ctggtcgaag cgccatcagt ggaagaattc aaaagaagat taaatgaaac
     7801 ttattacgga aaaaaatatg agttcggaga acaggtgcag attgagagta ttgtgaatga
     7861 atgtgtggaa catatcttaa cgatcgctta cagaaaccat ccgtattctc tggcaagtat
     7921 acaacaatat ttatttttaa aggaggaaga gatttataag atcaccacgg cactggaatg
     7981 tattcgctat ggattatcac aacgggagac attacagtat ctggtgcaaa agcaacgacg
     8041 tcaaggaggt aatgcatcat gatcgtaaag atgaaattct taagcatttc cggaccgaga
     8101 actgatattg acagggtaag tgcatcttac ctttccaaat acgaaatgca gctggaaaat
     8161 gcgatcacag aattgaaaac gacagataac ctcatgccgt ttatggatgt gaatccttac
     8221 aaagaaccgc tttctaaagc ggaacagtat gtattgatgc ttgcacagcc aaaaggagag
     8281 atggatacat ctcttacggc agatgagatc ctgacaatga tcagggatct caatcatgat
     8341 tatctggacc ttaaggcaag ggaagaccgg ttaaagaagc agaaagaaga attattagaa
     8401 aaacttcatg aacttgaacc gtttcagcct ttagagttaa atttaaaaga agtttatggc
     8461 tatcaatata tgaaagtcag attcggacga gtaaacgtag agtattatca gaaacttgaa
     8521 aaatatttat tcgatgatct caatgcaatt tttttagaag gaattcgaaa tgaaaattat
     8581 gtgtatggat gttattttgc ggcacataag gagatgggaa agattgattc tacctttaaa
     8641 tctctgcatt ttgaaaatat cgcacttcca gacgattacg aaggaatgcc ggcagagatt
     8701 tgcagaaatt taagaaatca gatcgatgag attgaaaaag aaatcgaaaa tgtccagaaa
     8761 cagatgaaag atttcttatc agtcaaggca aagaagattc gaggagcgag atataagtta
     8821 gaagaattat ccaataactt cgatgtgcgt aaaatggcag cctgtatgga aaatgaagaa
     8881 caggaagatt actacattct ctgtggatgg atgagtgaaa aagatacaca ggcatttatt
     8941 gaggagacaa aagatgatga taggatcacg atcattgttg aagaaggaag agaaaaattc
     9001 ttcggtgatc caccaacgaa attacagaat cccaaattct tcaaaccatt tgaaatgttt
     9061 atcagaatgt atggactgcc atcccatgat gagatggatc caaccatttt cgtggcactg
     9121 acttatacct ttatctttgg agcgatgttt ggggatgttg gtcagggatt gtgtttgttt
     9181 atcggcgggg gattactata taagatcaag aaatggaatc tagcaggaat catttctgtt
     9241 gccggaattt tttctacatt ctttggcttt atgtttggaa gcttctttgg atttgaagga
     9301 acgatcatta aaccaatgtg gttatcgcca atgcatgcga tgatgaaatt accgtttgtt
     9361 gggcagttaa atactgtatt tatcgtggca gttgcatttg gtatggcact gatcctgctt
     9421 gcgatggtat ttcagatcat caatgcaaag aaacgtgggg ataaagaaaa tttattcttc
     9481 agcccaaatg gagttgcagg acttgtattt tatggattcc ttgtcttaac gatcgtgctt
     9541 tatatgacag gtcataaaac accaggaaat atcatgatga tcattttcct tggaattcca
     9601 gtgatcatgt tcttattaaa agaaccatta ggacagctcg tggaaggaaa gaaaccaaaa
     9661 gcagaaggcg gcgttggaat gtttctggtg cagggatttt ttgaattgtt cgagacgatg
     9721 ttaagtttct tctctaacac gatctcattt gtgcgtatcg gggcatttgc agtgagccat
     9781 gcggctatga tggaagttgt tctgatgctc ggagggatca cagatggagc aggaaatcca
     9841 aactggatca tcattgttct tggaaatatt atcgtgtgtg gactagaagg tctggttgtc
     9901 ggaattcagg tattgcgtct ggaatattat gaaatgttca gtcgtttcta tacgggatca
     9961 ggaagagaat tccatccgta tgacaatcat gcaggcaagg aaaattagga ggtatcggtt
    10021 atgacattag caatcaaatt agcgatcatc gcagcattaa tcttaagtat atttattcca
    10081 tttggttatt ttcttcatgg agagagaaca aagaaacgtt ataaacgaag tattgcattt
    10141 aacgcttttt tcttcttcgg gatcattgtg atcgccggag tgatgatgtt tgtcacaaat
    10201 ccggtacagg cagcacaagc aggttctgat gcaggaatgg ctacaggact tggatatctg
    10261 gcagcagcat tatcaacagg actttcttgt gttggtggtg gtatcgccgt agcgagtgcc
    10321 gcaagtgcag ctttaggagc gatcagtgaa gatccaagtg ccttaggtaa atcactgatc
    10381 ttcgtaggtc tggcagaagg tgtatgtctg tatggattga tcatttcatt tatgatcatt
    10441 ggtaaacttg gatagatcag gaagtgatag ttatgaagat gtatttgatc agcgataact
    10501 tagatacatt gacaggaatg cggttagctg gggttgatgg gatcgtagtt catgaaaggg
    10561 atgaattaaa agaaacgatc gagaaagcta tgagtgatcc agagattggg atcgtgcttt
    10621 tgacagagaa atttggaaga gaatttccag aattgatcga cgagatcaag ctggagcgga
    10681 aaatgccgct tctgatcgag attcctgaca ggcacggaac tggacggaag aaagatttta
    10741 tcacgtctta tgtcaatgag gcgattggat tgaaattgta agccggctgt agaaggaagg
    10801 tgacaacatt gacagtagaa gaaaagattt cacatatcag agaagctgcc atggaagaag
    10861 caagagccag aggaaatgaa atcatagatc agcatcagaa ggcattggaa ggcgtgttca
    10921 aaacacataa acaagaagct gtgatgcagg cagatacgag gatcaagacc gaaacagcat
    10981 cggcaaggca gcagttaaat acagtaacat ccaaaggaca gttaaaactt cgaaggcagt
    11041 taagcagagt tcagaatgaa ttaaaaaata aattgtttga ggaagtccgg gaaatggcag
    11101 aagaatacat gaaaaccgaa gcgtataaag aattacttgt atcctatatt gcaaaagcgg
    11161 cgagattcgc agatgggaat ccattgacaa tctatattaa cagttcggat gaggacaaaa
    11221 aagaattcct cgaaaaacga actggaatga cagtaacagt cagcgaagaa gattttcttg
    11281 gaggaatccg ttcgattatt ccgggaagaa atatcttgat cgatcattca ttttcaggtg
    11341 ctttggagaa agaatacgaa gaatttacct ttaaaggggg tgtgacaggt gagtaagact
    11401 ggaacaattt atggaattaa cggacctgtc atttatctgg ctggaaatac tggatttaag
    11461 atgtcagaaa tggtttatgt cggcaaagaa aaattagttg gagaagtcat ttctctggat
    11521 aaagaccgta cgacgattca agtatatgaa gaaacatcag gattaaaacc gggcgagatc
    11581 gtggaagcaa gcggggaggc agtatccgtc acattagctc ccggaatcct tgataacatt
    11641 tttgatggaa ttgaacgtcc cttagagagg atcgcagaaa atgcaggtgc atttatcaca
    11701 agaggtatca gcgtagattc gcttgatatg gagaaattgt gggacacaaa attagttgtc
    11761 aatgaaggag atgttcttca tggcggtgat atctatgccc aagtgcagga aacaagagcc
    11821 atcgtacaca aatgcatggt gccgccagat cttacaggaa cggttacttt tgtggcatct
    11881 gatggagaac atgcgatcaa ggatcatatg atcacgttaa gattggacga tggaacggaa
    11941 aaacagttaa caatgatcca gagatggcca atcagggttc caagacctgt tcacgacagg
    12001 attccggcat gtgtgccact ggtaacaggc cagagaatac tggatacaat gtttccgatt
    12061 gctaaaggtg gaactgcggc aattccaggt ggttttggta ctggaaagac aatgacacag
    12121 catcagattg cgaaatggtc cgatgcggat attatcattt atatcggatg cggggaacgt
    12181 ggaaatgaga tgacacaggt attggaagaa ttttcagaat tagttgatcc aaagagtggt
    12241 aatccattga tggaccgaac aacactgatc gccaacacat ccaatatgcc ggtagcagca
    12301 cgtgaagcat ctatttatac aggacttacg ttggcagaat attaccgtga tatgggatat
    12361 gatgtcgcga tcatggcgga ttctacttca agatgggcag aagcgttaag agaattgtcc
    12421 ggtcgtctgg aagagatgcc agcagaagaa ggatttccgg catatttagc ttccagatta
    12481 tcagcttttt atgagagagc tggaatgatg cacaacttaa atggaacgga cggttctgta
    12541 acgatcatcg gtgcagtatc tccgcaggga ggagatttct cagagccagt tacacagaat
    12601 accaaacgat ttgtccgttg cttctgggga cttgataaat ccttggctta cgcaagacat
    12661 tttccagcga ttcactggtt aacgagttac agtgaatatc tgacagatct cggcggctgg
    12721 tacagagatc atgtatcccc caattttgtg gactatcgaa atcgtctgat ggcgatctta
    12781 aatcaggaaa gtagtttgat ggagatcgta aaactgatcg gtggggatgt tcttccagat
    12841 gatcagaaat taacgttaga gatcgcaagg gtgatccgat taggatttct gcagcaaaat
    12901 gctttccata aagatgacac ctgcgtgtcc atggagaagc aatttaaaat gatggaagtt
    12961 attttatatc tgtatcaaaa gagcagggaa cttgtagcga gaggaatgcc aatgagcgtg
    13021 ctaaaagcag aagggatctt tgagaaagtc attgcgatca aatacgatgt tccgaatgac
    13081 aatctgcaat tactggatct ctaccgtaaa cagattgatg atttctatga cgcagtactg
    13141 gaaaagaatg cataaggagg cggtcatttt atgggaattg aatatttagg actaagtagc
    13201 atcaaaggtc cgcttgtcat tctggaagga gtacaggacg cattttttga tgagattgtc
    13261 gaatttacgg ttgatggaaa aacgaaaaaa atcggaagga tcattgaatt atacgaagat
    13321 aaagcggtga ttcaggtatt tgagggaacg gaaaatatgt cccttgataa tacaagaaca
    13381 aaattaactg gacacccgat ggaagtctct cttgcaccag atatgctagg aagaacattt
    13441 aacggaatcg gaaaaccaat cgatggatta ggaccactga tcaccgatgt caaacgagat
    13501 gtcaacggcc ttccattaaa tccggtccgc agaaaatatc caagaaacta tatccgtaca
    13561 ggtatttcag caattgacgg attaacaaca ctgatccgtg gacagaaact tcccatcttc
    13621 tcaggaaacg gacttccaca cgatcagctg gcagcccaga tcgtaaaaca ggcatcctta
    13681 ggagatggtt cagatgagcc atttgcggta gtctttggag caatgggtgt caaacatgat
    13741 gttgcggatt tctttcagaa gacatttgag gaaagtggtg ttgcagatca tgtatgtatg
    13801 ttcttaaatc ttgcaaatga cccagttgta gaacgattga tcacaccaaa agtagcctta
    13861 actgcagcag aatatcttgc atttgactgt aatatgcata tcttagttat cctgacagat
    13921 atgacatcgt ttgcggaagc gatgcgtgag gtatcttcat cgaaaggaga aattccatcc
    13981 agaaaaggat atccaggata tttgtacagt gaacttgcaa cattatacga acgtgcagga
    14041 atcatcgaag gagcagaagg ttcggtcaca cagttaccaa tcttaactat gccaaatgat
    14101 gatatcactc atccaattcc tgacttaact ggttatatta cagaagggca gatcgtactg
    14161 gatcgtaatt taaacggaca gtccatttat ccaccaatca gtgttttgcc atcgctttct
    14221 cgtctgatga aagatggaat cggagaagga tacacaagag aagatcatca ggatcttgca
    14281 aaccagttat tttccgctta tgcgaaagtg ggagaagcaa gaaacttagc atctgttatc
    14341 ggagaagatg agttatcccc aattgataaa aaatatctgg aatttggaaa ggcatttgag
    14401 gaacgatata tcggtcaggg gccggaagaa aaccgttcca tgatcgaaac attggatctt
    14461 ggatgggaat tattaaagat cctgccaaga gaagaattgg atcgaatcga cacgaagatc
    14521 atcgataaat acctgccaaa agagcaggac agctaaaggt ggtgagacta tggacccacg
    14581 agaattcccg accaaaggaa atctgatcgc agccaagaac tctcttacat tggcaaaaca
    14641 gggttacgat ctgatggata aaaaaagaaa tatcctgatt cgtgagctga tggatctgat
    14701 cgatgaggcg aaagatattc aggaagagat tgatacaaca tttacaagag cgtatgcatg
    14761 ccttcagagg gcgaatatcc aacatgggat cagtaaggta gaagaacttg cttatacggt
    14821 tccaatcgag gattcgatac agattcagac aagaagtatc atgggaacag agattccaca
    14881 tgtgaaatat gatgcacatg agaatcagtt gacatatgcg atggatggga catttgaatc
    14941 aattgatgtt gccagggaag cgtttcggca ggtgaaggat ctgacaatta aattgtcaat
    15001 ggttgaaaac gcggcgtatc gtttagcaac tagtattaag aaaacgcaga agagggcaaa
    15061 tgcgttgaag aatattacga ttccgactta taccgagttg gtttataaga tttcgaatga
    15121 gttggaagag aaggagaggg aggaatttac gaggcttaag gtgattaagc gtatgaagca
    15181 gaaaaata
//
LOCUS       DAOMUE010000019        15009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_840_length_15009_cov_2.418951, whole genome shotgun sequence.
ACCESSION   DAOMUE010000019 DAOMUE010000000
VERSION     DAOMUE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 15009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15009
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_840_length_15009_cov_2.418951"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PLC71_01955"
     CDS             <1..1239
                     /locus_tag="PLC71_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0046358 - butyrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyryl-CoA:acetate CoA-transferase"
                     /protein_id="HRM01443.1"
                     /translation="TPVALDEALAKRMPDLENIQIHGGIVMRPLAITQIENPADHFTW
                     NSWHMGGLERKWINEGFSFYAPIRYSELPSYYRDCLPGTDVVMMQVAPMDAHGYFNFG
                     PSASHTAAMLEKAKCVIVEVNENMPRCLGGFEEGIHISKVDMIVEGNNPAIDELGGGG
                     AATEVDQAVARLIVDQIPDGACLQLGIGGMPNAVGSLIAESDLKDLGVHTEMYVDAFV
                     DIAKAGKITGSHKNIDRYRQTYAFAAGTKKLYDYLNDNPECMSAPVDYTNSIATISSI
                     DNFISINNAVDVDLYGQISAESSGIRQISGAGGQLDFVMGAYLSKGGKSFVCLSSTFT
                     NKKGETFSRILPTLHNGSVVTDTRSNAHYIVTEYGMANLKGKSTWERAEALINIAHPD
                     FRDQLIKDAETAHIWRRSNK"
     gene            complement(1332..2354)
                     /locus_tag="PLC71_01960"
     CDS             complement(1332..2354)
                     /locus_tag="PLC71_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HRM01444.1"
                     /translation="MEHIQKDDIIIRKGILHILDSHTGYLGLSSQLLEMGPDLMEFVR
                     GHIFKIMESDDAKRCQFNGSVSPVLSLLESMEESDDESFIEATRVLGENLFDIMCDSV
                     TIPAADLLCVTFQVQSVIHIALLKMNYKVTYVHREEEDAEANDIVKQRVMPTDSAKLT
                     EAVIIDLTEYKVRLVEKKYEMLNGDKINYLSERFLQCYADLAPKKKFQILNKVINDIN
                     NHYPEDGIAKRLDMKSRLREEFTENQAFKVNEIGDKLFGNHQGKKQEFDEKIERYDMQ
                     YDTFTVAKENTVKKLEYQMLETDTGIEIKIPMEEYNTKDNVEIIEEPGGGSTIIIKNI
                     ENVKIK"
     regulatory      complement(2770..2857)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2987..4021)
                     /locus_tag="PLC71_01965"
     CDS             complement(2987..4021)
                     /locus_tag="PLC71_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRM01445.1"
                     /translation="MGLEEYRGVYVFAQQVDNELSGIAFELLGKAKDLAKDLDTDVTA
                     VLVGSDVKGLVDDLAKYGADKVIVVDDPELKEYRTEPYTHALASVINEYKPEIMLVGA
                     TAIGRDLGPRVSARVQTGLTADCTVLEIGDFPLRAIPGKEQKHNQLLMTRPAFGGNTI
                     ATIACPDNRPQMATVRPGVMQKIDPIEGAKAEVIEYNPGFTPDNKYVEIMDIVKSVTD
                     TVDIMDAKILVSGGRGVGEEGFGMLKELAEVLGGEVSCSRAVVDNGWQPRDIQVGQTG
                     KTVRPNVYFAIGISGAIQHVAGMEESDIIVAINKDETAPIFDVADYGIVGDLNKIVPA
                     LTEKLRAVVK"
     assembly_gap    4100..4109
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4110..4832)
                     /locus_tag="PLC71_01970"
     CDS             complement(<4110..4832)
                     /locus_tag="PLC71_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRM01446.1"
                     /translation="MKIVVCVKQVPDTKGGVKFNPDGTLDRGAMLTIMNPDDKAGLEA
                     ALRIKDEYGAEVTVLTMGLPKADEVLREAMAMGADKGILVTDRVLGGADTWATSTTLA
                     GALRNLDYDLIITGRQAIDGDTAQVGPQISEHLDIPVISYAEDIKIDGDSVVVQRQYE
                     DRYHILKAKMPCLVTALAELNEPRYMTPGGIFDAYDAEVTVWGRKDLKDVEDSNLGLA
                     GSPTKIAKASDKVRKGAGEKVTL"
     gene            complement(4846..5997)
                     /locus_tag="PLC71_01975"
     CDS             complement(4846..5997)
                     /locus_tag="PLC71_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HRM01447.1"
                     /translation="MDFTLSKKHEMARTLFKEFAEKEVKPLAQDVDENEQFPVETVAK
                     MAKAGFLGIPVPKEYGGQGCDPLTYVMCVEELSKVCGTTGVIVSAHTSLCIDPIQTFG
                     TEEQKKKYIPDLASGKKLGAFGLTEPGAGTDAQGQQTKAVLDGDEWVLNGSKCFITNG
                     SYADVYIVIAVTGVVEKRGRKMKEISAFIVEKGTPGFTFGTKEKKMGIRGSATYELIF
                     EDCRIPKENLLGKQGKGFGIAMHTLDGGRIGIASQALGLAEGALERTIEYVKERKQFG
                     RSIGQFQNTQFQLADMATKVEAAKQLVYKAAMAKATQKVYSVEAAMAKLYAAEVAMEV
                     TTKAVQLHGGYGYIREYDVERMMRDAKITEIYEGTSEVQRMVISGNLLK"
     gene            complement(6085..6924)
                     /locus_tag="PLC71_01980"
     CDS             complement(6085..6924)
                     /locus_tag="PLC71_01980"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HRM01448.1"
                     /translation="MKVGVIGAGTMGSGIAQAFAQTEGYEVCLCDINEEFAANGKAKI
                     AKGLEKRVARGKMEQAAADAILAKITTGVKDICTDCDLIVEAAIENMEIKKQTFKELQ
                     DICKEDAIFATNTSSLSITEIGAGLDRPMIGMHFFNPAPVMKLVEVIAGINTPKEVVD
                     KIKAIAEEIGKTPVQVEEGAGFVVNRILIPMINEAIGIYADGVASVEGIDTAMKLGAN
                     HPMGPLALGDLIGLDVCLAIMEVLYNEFGDTKYRPHPLLRKMVRGGLLGCKTGKGFYD
                     YSK"
     gene            complement(7012..7803)
                     /locus_tag="PLC71_01985"
     CDS             complement(7012..7803)
                     /locus_tag="PLC71_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004852940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRM01449.1"
                     /translation="MEFVTYEQDGFVGVVTINRPKALNALNSEVLKEIEATFDAIDLD
                     ATRAVVLTGAGDKSFVAGADIGEMSTLTKAEGEAFGKTGNDVFRKIETFPIPVIAAVN
                     GFALGGGCEISMSCDIRICSENAIFGQPEVGLGITPGFGGTQRLARIVGVGKAKEMIY
                     TAFNIKAEEAYRIGLVNAVYPQEELLAAAKKLANKIASNAPIAVRACKKAINEGLQVD
                     MDQAIVVEEKAFGSCFETEDQKAGMGNFLEKDKAKKLKVVPFQNK"
     gene            complement(7840..9024)
                     /locus_tag="PLC71_01990"
     CDS             complement(7840..9024)
                     /locus_tag="PLC71_01990"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HRM01450.1"
                     /translation="MAKKVVIAGACRTAIGKMGGVLSTVPAVDMGSIVIKEALKRAGV
                     PADQVDHVYMGCVIQAGLGQNVARQASLNAGLPIETPAVTVNVVCGSGLNCVNMAAQM
                     IQAGDADIVVAGGMESMSMAPFALPKARFGYRMNNGTLVDTMVNDALTDAFNHYHMMI
                     TAENVAEQWGLTREELDEFAAASQQKAVAAQESGRFKDEIVPVEVKQRKKTVIVDTDE
                     GPRAGTTAESLAGLRCCSGKEGGVVTAGNASGINDGAAAVVVMSEEKAKELGVTPMAT
                     YVTGALGGVDPSIMGVGPVASTKKALAKADLTIDDMDLIEANEAFAAQSVAVARDLKF
                     DMSKVNVNGGAIALGHPVGASGCRIFVTLLHEMQKRDAKKGLATLCIGGGMGCTTIVE
                     RD"
     gene            complement(9570..10079)
                     /gene="ilvN"
                     /locus_tag="PLC71_01995"
     CDS             complement(9570..10079)
                     /gene="ilvN"
                     /locus_tag="PLC71_01995"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRM01451.1"
                     /translation="MDNQMERRWLSLFVENRAGVLARISGLFSGKAYNIHSLTVGETE
                     DPTMSRMTICIDADEKTFEQVKKQLNRSVEVIKVIDYTDTSIRTKEIMFIKVKSLTEK
                     QMAEIFQMAEVFRFLIKDMGKDSLLIEAIRTETKNDSILKVFKERYTNIEVVRGGPVA
                     IDCISMQDR"
     gene            complement(10091..11836)
                     /gene="ilvB"
                     /locus_tag="PLC71_02000"
     CDS             complement(10091..11836)
                     /gene="ilvB"
                     /locus_tag="PLC71_02000"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRM01452.1"
                     /translation="MGKMTGNEFLVKALQEEGVEKIFGYPGACVIDIFDEIYKQDQVE
                     LILPRHEQGLIHAADGYARATGKVGVCLATSGPGATNLVTGIATANYDSVPLVCFTGQ
                     VATNLIGNDTFQEIDIVGVTRNIAKFVIVVRKREDLNRLIKEAFYIARTGKPGPVLLD
                     LPKDVMAELGSDEYPEEVNIRGYKPNTKPHHGQILRAMNMIYDAKRPLFLIGGGAKIA
                     GAQEEMCKLMETLKVPVVTTIMGRGIVPTNHELYYGNIGMHGNYAANQAVNDCDLLIS
                     IGTRFNDRITGKLGTFASDAKIIHIDIDTAAISKNVTVDVPLVGDAKEAIALMLSIAE
                     NEEPCKVDEWLSRMNEWHEQKPLVMEEKEKLVPKQVIDAINEYFDEPIVTTDVGQNQM
                     WTTQFLELYGRRQMLTSGGLGTMGYGLPAAIGAAIGNPGREVVCVSGDGGFQMNSQEI
                     ATAVIQELPITICILNNGYLGMVRQWQDLFYDKAYAGTCLRRRKSCEHHCDGDFSKCP
                     PYTPDFVKLAESYGAQGIRVFKKEEIIPAFEKAKAKTDGPTIIEFIIENEELVMPMVK
                     PNGSITDLIMERGEE"
     gene            complement(11838..12431)
                     /gene="scpB"
                     /locus_tag="PLC71_02005"
     CDS             complement(11838..12431)
                     /gene="scpB"
                     /locus_tag="PLC71_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRM01453.1"
                     /translation="MKNKLAAIEGILFSMGTSVTRRQLMEALEIDNEQFDELIKLLQT
                     EYDKEERGIRLLELDDAYQLCTKTEYYGSLIRIVNHPKKPRLTDVMLETLSIIAYKQP
                     VTKQEIEAIRGVKCDHPINKLLEYRLIKEAGRLDAIGRPILFRTTEEFLRCFGVKSTD
                     DLPVVGEEQVEDFKIQAQEEVKYTLDETSLEEEMEEM"
     gene            complement(12439..13179)
                     /locus_tag="PLC71_02010"
     CDS             complement(12439..13179)
                     /locus_tag="PLC71_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRM01454.1"
                     /translation="MGIDIKVNAFEGPLDLLLHLIEKNKVDIYDIPISEITSQYLDYI
                     RSMEEEDLDVMSDFLVMAATLLKIKAKMLLPVKEEEEEEGDPREELVRRLIEYKIYKY
                     ASLQLKEREMMAEKSFFRSPDIPPQVLAYREEIDPVQMLSEVTLQRISEVFQFVMRKR
                     QDKLDPIRSEFGEIKQEEIKLEDKITEVYNYIIQYKEVNFYDLLNEQETKEAVIVTFL
                     AVLELMKTGKIYVQQENIEDDIFIRALE"
     gene            complement(13183..14067)
                     /locus_tag="PLC71_02015"
     CDS             complement(13183..14067)
                     /locus_tag="PLC71_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM01455.1"
                     /translation="MIPLWIWIIAVLLVVGIAVAVLLSSYENKRLTVEYYEIESEKIP
                     EAFDGYRIVFLTDLHCAQFGENNQELIERIDECRPDMILVGGDMVISRESSNEEVPLA
                     LMKELSAKYPIYYADGNHELKLARNEEIFGVRYKDYVHSLKEYNIHHISNETIVIQSE
                     TGFISLTAFDLEKKYYQRFHVPRMPLSHMESVVGSSPGNIFHILLAHNPNYLKTYADW
                     GSDLVLAGHFHGGMVRIPKFGGVISPQFQIFPKYDAGEFHEGETTMILSRGLGNHSIK
                     LRLFNKPEISCIELKKRD"
     gene            complement(14257..14610)
                     /locus_tag="PLC71_02020"
     CDS             complement(14257..14610)
                     /locus_tag="PLC71_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HRM01456.1"
                     /translation="MYQVTIKGKGAMVDAFREEGIFVTFGDNAPDTLKDFCYSIDVNP
                     VDGEVTPGCKLVIDGKEFKIVKVGEVANENLKNLGHVTYNFGGEDAECLPGTICMEKA
                     ELPALDIGSTIAIVK"
     gene            complement(14644..>15009)
                     /locus_tag="PLC71_02025"
     CDS             complement(14644..>15009)
                     /locus_tag="PLC71_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HRM01457.1"
                     /translation="AKLLQPLCGNIIGLVILGFICSIPFLSCLLGPGAVIAQIVGTLI
                     GVEIGKGNIEPSLALPALFAINTQCACDFVPVGLGLAEADPETVEVGVPAVLYSRFIT
                     GVPRVIVGYLASFGLYAAK"
BASE COUNT         4054 a         3460 c         2326 g         5159 t
ORIGIN      
        1 actccagttg ctttagacga agctttagca aaacgtatgc ctgatcttga aaatattcag
       61 attcatggtg gtatcgttat gcgtccatta gcaatcactc agattgaaaa cccagcagat
      121 cattttacat ggaattcatg gcatatgggt ggtttagaaa gaaaatggat caacgaagga
      181 ttctctttct acgctccgat ccgttattct gaattaccaa gctattaccg tgattgtctt
      241 ccaggaacag acgttgttat gatgcaggtt gcacctatgg atgctcatgg atattttaac
      301 tttggtccaa gtgcttctca tacagcagct atgcttgaaa aagcaaaatg cgttatcgtc
      361 gaagttaatg aaaatatgcc tcgttgcctt ggtggtttcg aagaaggtat ccatatttcc
      421 aaagttgaca tgatcgttga aggaaacaat cctgctattg acgaattagg tggtggcgga
      481 gctgctacag aagtggatca ggcagttgca agactgatcg ttgatcagat tcctgacgga
      541 gcctgcttac agcttggaat cggtggtatg cctaatgctg taggatcttt gatcgcagaa
      601 tccgatttaa aagaccttgg tgttcataca gaaatgtatg ttgatgcttt cgttgatatt
      661 gcaaaagccg gtaagatcac aggttctcac aagaacatcg accgttaccg tcagacttat
      721 gctttcgcag caggtacaaa aaaattatat gattatttaa atgataaccc agagtgcatg
      781 agcgctcctg ttgattatac aaacagcatc gctacgattt catctatcga taactttatc
      841 tctatcaaca atgcggtaga tgttgactta tatggacaga tcagtgctga gtcttcagga
      901 atccgtcaga tcagtggtgc cggtggacag cttgatttcg ttatgggtgc ttacttatct
      961 aagggtggaa agagttttgt atgcttatct tctactttca caaataagaa aggggaaaca
     1021 ttctcaagaa tccttcctac attacataat ggatctgtcg ttacagatac tcgttcaaat
     1081 gctcattaca tcgttacaga atatggtatg gcaaacttaa aaggtaagtc tacatgggaa
     1141 cgtgcagaag ccctgatcaa tatcgctcat ccagatttca gagatcagct gatcaaagat
     1201 gcagaaactg ctcatatctg gagaagatcc aacaaataag ctaacaatcg attattttaa
     1261 taattgtttt ttaaattttc caaaccaaat aaaaatcccg gaccttttac ggatccggga
     1321 tttttatttg gttatttaat ttttacattt tctatattct taatgatgat cgtactgcct
     1381 ccgcctggct cttcaatgat ctcaacatta tctttggtat tatattcttc catcggaatc
     1441 ttgatctcaa ttcctgtatc cgtctctaac atctgatatt ccagtttctt gactgtattt
     1501 tcttttgcta ctgtaaatgt atcatattgc atatcatatc gttcaatctt ctcatcgaat
     1561 tcctgcttct taccttgatg atttccaaat agtttatccc cgatctcatt caccttaaac
     1621 gcctgatttt ccgtaaattc ttctcttaat cggcttttca tgtccaaacg tttcgcaatc
     1681 ccatcttctg gataatggtt gtttatatcg ttaataactt tatttagaat ctgaaatttc
     1741 ttctttggtg ccagatctgc atagcattgt aagaatctct cagaaagata gttgatctta
     1801 tccccgttca acatctcata ctttttctct acaagacgta ctttgtattc tgtcagatcg
     1861 atgatcactg cttctgtaag ctttgccgaa tccgttggca taactctttg tttcacgata
     1921 tcatttgctt ctgcatcttc ctcttctcga tgtacataag tcactttgta attcatctta
     1981 agcagtgcga tatgaattac actttgtacc tgaaaagtca cacaaagaag atccgctgca
     2041 ggaatcgtta cactatcaca catgatatca aacagatttt ctcctaaaac tcttgtcgcc
     2101 tctataaagc tctcatcatc tgattcttcc atgctctcta acaaacttaa tactggcgaa
     2161 acacttccat tgaactgaca tctttttgca tcatcacttt ccataatttt aaaaatatgt
     2221 cctctgacaa attccattaa atctggtccc atctccaaaa gctgactgga taagccaaga
     2281 tatcctgtgt ggctgtccaa aatatgtaaa attccttttc tgataataat atcatctttc
     2341 tgtatatgtt ccatgattga ttgtctcctt tttgttttat ccattgtgta tcattttatc
     2401 atatcgatca tttttacgca aaaagaaaga atcctgctat acagaattct tctacgaaaa
     2461 taaaaagtca tggttcccta caccatgact tcttctaata tgaaaaatcc ccatgtattc
     2521 ccaccaatga aggatcggca accccttgcc ggtgatctta ctgtgaaccc cttcacaaat
     2581 aaaatctgtg gaaaacataa aatttcatat tctaatagga ccaacttctg atattctcct
     2641 cggtatagca gattctatta tctggctccc tacttcctga caacaaaaaa ccgttcatag
     2701 aattatctct tctataacgg ctgcctctat ctggattaaa tacccatatc acattccctt
     2761 aatgctaaat gtggcaaccg gctgtcatag tccaataaga ccttagaccc ttctggcttt
     2821 gcgtccctgc ctttcaacag gtttgcccta tgaataaata tgaactttta actatgcata
     2881 tagaatatca cactttccat aatctgtcaa ttatttttaa caatacataa agtccgcgga
     2941 agtattgatc cgcggactct ttaaaaatta aattctcaaa aaatgattat ttaacaactg
     3001 ctcttaattt ttctgttaaa gctggaacga tcttgtttaa gtctccaaca attccgtaat
     3061 cagctacatc aaagatagga gctgtttcat ctttgttgat tgcaacgatg atatcagatt
     3121 cttccatacc tgcaacgtgc tggattgctc cagaaatacc aattgcgaag tatacgttag
     3181 gacgtacagt ttttcctgtc tgtccaacct ggatatctct tggctgccat ccgttatcaa
     3241 caacggcacg agaacagctt acttctcctc cgagaacttc agctaattct tttaacattc
     3301 caaatccttc ttctccaact ccacgtccac cagatactaa gatcttagca tccatgatat
     3361 ctactgtatc tgtaacagat ttaacgatat ccatgatttc aacgtatttg ttgtctggag
     3421 taaatccagg attgtattcg ataacttcag cttttgctcc ttcaatagga tcaatcttct
     3481 gcataacacc aggacgaact gttgccatct gaggtctgtt gtctgggcaa gcgattgttg
     3541 cgatcgtatt tccaccgaat gctggacgag tcattaataa ctgattgtgt ttctgttctt
     3601 ttcctgggat tgctcttaat gggaaatcac cgatttcaag aactgtacag tcagctgtta
     3661 aacctgtctg aacacgtgca gatacacgag gtccaaggtc acgaccgatt gctgtcgcac
     3721 caactaacat gatctctggt ttgtattcat tgatcacaga tgctaatgca tgtgtatatg
     3781 gttctgttct gtattctttt aattctggat cgtcaactac gataacttta tcagcaccgt
     3841 attttgcaag atcatctact aatcctttaa cgtcagaacc tacaagaaca gcagttacgt
     3901 ctgtatctaa gtcttttgct aagtcttttg cttttccaag taattcgaat gcgattccgc
     3961 ttaattcgtt atctacctgc tgtgcaaaga cgtatacgcc tctatattct tctaaaccca
     4021 ttttattcac cacttttcct tttcactaaa ttacatgttt ttcagctaat ttatccatga
     4081 tataatctac tgcttcttcn nnnnnnnnna agtgtaactt tttctccagc accttttctt
     4141 actttgtcag atgcttttgc gatcttagtt ggagatccag caagaccaag gttagaatcc
     4201 tctacatctt taaggtcttt tcttccccaa acagttactt ctgcatcata tgcatcaaaa
     4261 attccacctg gagtcatata tcttggctcg tttaattcag ctaatgctgt aactaagcaa
     4321 ggcattttag cttttaagat gtggtatcta tcttcatact gacgctgaac aactacgctg
     4381 tcaccgtcaa ttttaatgtc ttctgcataa gagataactg gaatatcaag atgttcagaa
     4441 atctgtggtc caacctgagc tgtatcacca tcgatagcct gacgtcctgt gatgatcaga
     4501 tcatagtcaa gatttcttaa tgctccagct aatgtagtag aagttgccca tgtgtcagct
     4561 cctcctaata ctctgtcagt tacaaggata cctttatcag cacccattgc catagcttca
     4621 cgaagaactt cgtcagcttt tggaagtccc attgtaagaa ctgtaacttc agctccatat
     4681 tcatctttaa ttctaagtgc tgcttctaat ccagctttgt catctgggtt catgatagta
     4741 agcatagcac ctctgtctaa tgtaccatct gggttgaatt taactccacc ttttgtatct
     4801 ggtacctgtt ttacacaaac aactatcttc acggtaagta cctccctatt tcaataagtt
     4861 tccagaaata accatacgct gaacttcaga tgttccttcg tagatttctg tgatcttagc
     4921 atcgcgcatc atacgttcta catcgtattc tctgatgtat ccgtaaccac catgtaactg
     4981 aacagctttt gttgttactt ccatagcaac ttctgctgca tataatttag ccatagcagc
     5041 ttcaacactg tatactttct gagtagcttt agccatagca gctttgtata ctaactgttt
     5101 tgcagcttct acttttgttg ccatatcagc taactggaac tgtgtattct ggaactgtcc
     5161 gatagaacgt ccgaactgtt ttctttcttt tacatattcg attgttcttt ctaaagcacc
     5221 ttctgcaagt ccaagagcct gagaagcgat accaatacgt cctccatcaa gagtatgcat
     5281 tgcgataccg aaacctttac cctgttttcc taacagattt tcttttggaa tacggcagtc
     5341 ttcaaaaatt aattcatatg tagctgatcc gcggataccc attttctttt cttttgttcc
     5401 gaatgtgaaa cctggagttc ctttttctac gatgaatgca gaaatttctt tcatctttct
     5461 tccgcgtttt tcaacaacac ctgttactgc gattacgata taaacgtctg cgtagctacc
     5521 atttgtaatg aagcacttag atccgtttaa tacccattcg tctccgtcta atacggcttt
     5581 tgtctgctgt ccctgtgcat ctgtaccagc acctggttct gtcagaccaa aagctcctaa
     5641 tttcttacca gatgctaaat ctgggatata ttttttcttc tgttcttctg taccaaaagt
     5701 ctggattgga tcgatacaaa gagatgtatg tgcagaaacg ataacacctg ttgtaccaca
     5761 aacttttgat aattcttcaa cacacataac gtatgttaat ggatcgcatc cctgtccacc
     5821 atattcttta ggaactggaa tacctaagaa tcctgcttta gccatttttg ctacagtttc
     5881 aactggaaac tgttcatttt catccacgtc ctgtgcaaga ggctttactt ctttttcagc
     5941 aaactctttg aaaagagttc ttgccatttc atgtttctta cttaatgtga aatccattct
     6001 ttattcctcc atcatgattt ctataaagtt aacttctgca tccggagttc accatctccg
     6061 gatgcaaaag aaaagctctt tttcttactt gctgtaatcg tagaatcctt taccagtctt
     6121 acatcctaat aatcctccac gaaccatctt tcttaataat ggatgtggac gatattttgt
     6181 atcaccaaat tcattgtaaa gtacttccat gattgcaagg caaacgtcta atccaattaa
     6241 atcacctaaa gctaaaggtc ccattggatg gtttgctcct aatttcatag ctgtatcaat
     6301 accttctaca gaagctactc catcagcata aattccgatt gcttcgttga tcatagggat
     6361 taagattctg ttaactacga atccagcacc ttcttcaacc tgtacaggtg ttttaccgat
     6421 ttcttcagcg attgctttga tcttatcaac aacttctttt ggtgtgttga taccagcgat
     6481 aacttcaacc agtttcataa caggagctgg gttaaagaaa tgcataccga tcattggacg
     6541 atcaagtcct gcaccaattt ctgtgattga taaagaagat gtatttgtag caaagatagc
     6601 atcttcttta cagatatcct gtaattcttt gaatgtctgt tttttgattt ccatgttttc
     6661 aatagctgct tctacgatta aatcgcagtc tgtacagata tcttttacac ctgttgtgat
     6721 ctttgctaag atagcatctg cagctgcctg ttccattttt cctcttgcaa cacgtttttc
     6781 taaacctttt gcgattttag cttttccgtt tgctgcaaat tcttcattaa tatcacataa
     6841 gcatacttca tatccttctg tctgtgcaaa tgcctgtgcg attccgctac ccattgttcc
     6901 agcgccgatt actccaactt tcattataat tcctcctgta aaattatttt actatgtaat
     6961 tttccggtct gcggctgata ccgcagactg gaaatatatt aaacttcatt attatttgtt
     7021 ctggaaagga acaactttta atttcttagc tttgtctttc tctaagaagt tacccattcc
     7081 tgctttctgg tcttctgttt caaaacagct tccaaaagct ttttcttcaa caacgattgc
     7141 ctgatccata tctacctgaa gaccttcgtt gattgctttc ttacatgcac gtactgcgat
     7201 tggtgcatta gatgcgatct tgttagctaa tttcttagct gctgctaata attcttcctg
     7261 tggatatact gcattcacaa gtccgattct gtaagcttct tcagctttga tgttaaatgc
     7321 tgtatagatc atttctttgg cttttccaac tccgacgata cgagcaagtc tctgtgtacc
     7381 accgaatcct ggtgtgattc caagaccaac ttcaggctgt ccaaagattg cgttttcaga
     7441 acagattctg atatcacaac tcatggaaat ctcacatcca ccacctaaag cgaatccatt
     7501 gactgctgcg ataactggaa tagggaatgt ttcgatcttg cggaatacat cgtttcctgt
     7561 cttaccgaaa gcttctcctt ctgcttttgt taatgtactc atctctccga tgtcagcacc
     7621 ggcaacgaaa gatttatctc cagcacctgt taatacaacg gctcttgttg catctaagtc
     7681 gatcgcatca aaagttgctt cgatttcttt taatacttca ctgtttaatg cgtttaatgc
     7741 ttttggacga ttgattgtta caacacctac aaaaccgtcc tgttcatatg taacgaattc
     7801 cattctaaga attctccttt cctaattatc tataattaat tagtctcttt caacgatagt
     7861 tgtgcaaccc attccaccac cgatgcaaag tgttgcaaga cctttcttag cgtctctctt
     7921 ctgcatttca tgaagcagtg taacgaagat acggcatcct gaagctccta ctggatgtcc
     7981 aagagcgatt gctccaccgt ttacgtttac tttgctcata tcgaatttca gatctcttgc
     8041 tactgctaca gactgtgctg cgaaagcttc gtttgcttcg attaagtcca tatcgtcaat
     8101 tgttaagtct gcttttgcta aagctttctt tgtagaagct actggtccaa cacccatgat
     8161 agatggatct acgccaccaa gagctcctgt tacatatgta gccattggtg taacacctaa
     8221 ttctttggct ttttcttcgc tcattactac gactgctgct gcaccatcgt tgattcctga
     8281 tgcgttacca gctgtaacaa ctccaccttc ttttccagag cagcatctta atcctgctaa
     8341 agattcagct gttgttccag ctcttggtcc ttcgtctgta tctacgataa ctgttttctt
     8401 tctctgtttt acttctacag gaacgatctc atctttgaat cttcctgatt cctgagctgc
     8461 gactgctttc tgctgagaag ctgctgcaaa ctcatctaat tcttcacgag ttaatcccca
     8521 ctgttctgct acgttttcag ctgtgatcat catgtgatag tgattgaatg catctgttaa
     8581 tgcatcgtta accattgtat ctaccagtgt tccgttattc atacgatatc cgaaacgtgc
     8641 ttttggtaat gcaaatggag ccattgacat gctttccata ccaccggcaa caacgatatc
     8701 agcatctcct gcctgaatca tctgtgccgc catgtttaca cagtttaatc ctgatccaca
     8761 tactacgtta actgtaactg ctggtgtttc aattggaagt cctgcgttta aggaagcctg
     8821 acgagcaacg ttctgtccta atcctgcctg gataacgcat cccatgtata catggtctac
     8881 ctgatcagct ggaactcctg ctctttttaa tgcttctttg attacgattg atcccatgtc
     8941 tactgctggt actgtgctga gtactccacc cattttacca atcgctgtac ggcatgctcc
     9001 tgcgataact actttctttg ccatatcaat tttcctccat attcttgatt attatacttt
     9061 ggcgatcttc tctctcgccg atatgttaat actatcacaa tcttttctca ttttcaattg
     9121 ctatttttta aaaagatttt caataaattg tgcaaagtgc caaaagccgc ataaatacgt
     9181 gttttttcga tgtatacaat gtccttgtcg gattgtattt tttttaacac aatccgacat
     9241 aacttataac actttctcac cgaattgata actttcttgt attttctatg aaataatcct
     9301 gattttttcg tgaaaaaaat aacaattttt acaatataaa tatatttaca ctctttatag
     9361 attgattttt tgttaaattt acttcacttt caacatgtct aacttaacac atccctgtat
     9421 tttttcattg aaagaaaaaa ctttttattc ctatgaaaaa tccccatatt ttttaaaaac
     9481 ttatttctgt acacgcaaaa aagagagctt ggtgtgtgcg ttaaaacacc aaactctctt
     9541 ttctcttcta tattattgtc ttctgatcat taacgatcct gcatactgat acaatcgatc
     9601 gctaccggac cacctctgac tacttcaata tttgtataac gttctttaaa cacctttaaa
     9661 atagaatcat tctttgtctc tgtacgaatt gcttctatta ataagctgtc ttttcccata
     9721 tccttgatca ggaaacggaa cacttctgcc atctggaaga tctctgccat ctgcttctct
     9781 gtaagacttt tcactttgat aaacatgatt tcctttgttc ggatcgatgt atctgtatag
     9841 tcaataactt taataacttc cacgcttcga tttaactgtt tcttgacctg ttcaaatgtc
     9901 ttttcatctg catcaataca gatcgtcata cgagacattg ttggatcttc tgtttctcca
     9961 actgtaagac tgtgtatatt gtaggctttt ccagagaata gtccggaaat tcttgcaaga
    10021 acaccagcac ggttttctac gaacaaggaa agccatcttc tttccatctg attatccatt
    10081 ttcattcctc ctattcttct ccacgttcca tgatcagatc tgtgatagat ccatttggtt
    10141 tcaccattgg cataacaagt tcttcattct caatgataaa ttcaatgatc gttggcccat
    10201 ctgttttcgc ctttgctttc tcaaatgctg ggataatttc ttctttctta aagactctga
    10261 tcccctgtgc tccataactt tcagcaagtt ttacaaaatc tggtgtatat ggcggacatt
    10321 ttgaaaaatc tccatcacaa tgatgttcac aactctttct tcttcttaaa catgtacctg
    10381 catatgcttt atcatagaag agatcctgcc actgtctcac cattccaaga tacccattat
    10441 ttaaaataca gattgtaata ggaagttcct gaatcacagc tgtcgcgatc tcctgggaat
    10501 tcatctgaaa tccaccatct ccagatacac aaacaacttc tcttcctgga ttaccaattg
    10561 ctgcaccaat tgctgctgga agcccataac ccatcgttcc gagtccacca gatgtcagca
    10621 tctgtcgtct tccatatagt tctaagaact gtgtcgtcca catctgattc tgtccaacgt
    10681 ctgttgtaac aattggttca tcaaagtact cattgatcgc atcaataacc tgttttggaa
    10741 ctaatttttc tttttcttcc attactaatg gcttttgttc atgccattca ttcattcttg
    10801 acagccattc atctacttta catggttctt cattttctgc aatagacaac attaatgcga
    10861 tggcttcttt tgcatctcct accaatggca catcaaccgt aacgttctta gaaatcgccg
    10921 ctgtatcaat atcaatatga atgatcttgg catccgatgc aaatgttcca agttttcctg
    10981 taattcggtc attaaatctt gttccaatgg aaatcaacag atcacaatca tttactgcct
    11041 gatttgctgc ataatttcca tgcattccaa tatttccata atataattca tgatttgttg
    11101 gtacgatccc tcgtcccatg atcgttgtaa caactggaac ctttaatgtt tccattaatt
    11161 tgcacatctc ttcttgtgcc cctgcgatct tcgcacctcc accaattaag aataatggac
    11221 gtttcgcatc atagatcata ttcattgcac gaagaatctg tccatgatgt ggtttcgtgt
    11281 ttggtttata tccacggata ttgacttcct ctggatattc atcacttcct aattctgcca
    11341 tcacatcttt tggaagatct aataagactg gtcccggttt ccctgttctt gcaatataaa
    11401 atgcctcttt gatcaggcga ttcaaatcct ctcttttacg gacaactata acaaatttcg
    11461 cgatatttct tgtaactccg acaatatcga tctcctgaaa tgtatcatta ccgatcagat
    11521 ttgtggcaac ctgtcctgta aaacacacca atggcacact gtcataattc gctgttgcta
    11581 ttcctgtaac aaggtttgtg gcacctggtc cacttgttgc cagacagaca ccaacttttc
    11641 ctgtagctct tgcatatcca tctgccgcat ggatcaaccc ctgctcatgt cttggaagaa
    11701 tcagttctac ttgatcctgc ttatagattt catcaaaaat atcaatgaca cacgcacccg
    11761 ggtatccaaa gatcttctct acgccttctt cctgtaatgc ctttaccaga aattcatttc
    11821 ctgtcatttt acccatacta catctcctcc atctcttctt ccagactagt ctcatctagt
    11881 gtatatttca cttcttcctg agcctgtatt ttaaaatctt ccacctgttc ttctccaaca
    11941 actggaagat catctgttga ttttactcca aaacatctca aaaattcttc tgtcgtacga
    12001 aacaagatcg gacgcccgat cgcatccagc cgtccagcct ctttgatcag ccggtattct
    12061 aacagcttgt tgatcggatg atcgcatttc acacctcgga tcgcttcaat ctcttgtttc
    12121 gttactggct gcttataagc aatgatcgat aaagtctcaa gcattacatc cgtaagcctt
    12181 ggtttctttg gatgattcac aattcttatc aatgatccat aatattctgt tttggtacaa
    12241 agctgataag catcatccag ttccaataac cggattcctc tctcttcttt gtcatattct
    12301 gtctgtaaca gtttgatcaa ctcatcaaac tgttcattat ctatctcaag tgcctccatc
    12361 agctgccgcc ttgtcacact tgttcccatc gaaaataaaa ttccttcgat cgctgcaagt
    12421 ttattcttca taaattcatt actccagtgc ccgtatgaaa atatcatctt cgatattttc
    12481 ctgttgaacg taaatctttc ctgtcttcat caattcaaga accgccagaa atgtgacgat
    12541 cactgcttct tttgtctcct gttcgtttag aaggtcataa aagttcactt ctttatattg
    12601 tataatataa ttatacactt ccgtgatctt atcttctaat ttaatctctt cctgcttgat
    12661 ctccccaaat tcgcttcgga tcggatctaa cttatcctgt cgttttctca tcacaaactg
    12721 aaaaacttca gaaatcctct gtaaagttac ctctgataac atctgcacag gatcgatctc
    12781 ttctctgtat gcaagtactt gcggtggaat atctggtgat ctaaaaaaac tcttctctgc
    12841 catcatctct cgttctttca actgtaaaga ggcatattta taaatcttat attcaatcag
    12901 tcttcttaca agttcttctc tcggatctcc ttcttcttcc tcttcttcct tcactggaag
    12961 caacatcttc gccttgatct ttaataatgt ggctgccata acgagaaaat cactcatcac
    13021 atcaagatct tcctcttcca tagagcggat ataatcaaga tactggcttg tgatctcaga
    13081 gattggaata tcataaatat ccactttatt cttctcgatc agatgcaaca gaagatctaa
    13141 tggtccttca aatgcattta ccttaatatc tattcccata aatcagtctc tcttctttaa
    13201 ttcaatgcat gaaatctctg gtttattaaa caatcttaat ttgatggaat ggtttccaag
    13261 tccacggctt aagatcatcg ttgtctctcc ctcatgaaac tctcctgcat cgtattttgg
    13321 aaaaatctga aattgcggtg agatcacacc tccaaatttc ggaattctta ccattcctcc
    13381 atggaaatga ccagctaaga caagatcact gccccaatct gcatatgttt ttaaatagtt
    13441 tgggttatgt gctaataaaa tatgaaaaat gtttcctgga gaacttccta ccacagattc
    13501 catatgagaa agcggcatgc gcggtacatg aaatctctga taatatttct tctcaagatc
    13561 aaacgcagtt aaagaaataa atccagtctc tgactgaatt acgatcgttt cattcgaaat
    13621 gtgatgtatg ttatactctt tcagactatg aacataatct ttataacgca caccaaagat
    13681 ttcttcattc cttgcaagtt tcaattcgtg gtttccatcc gcataataga tcggatactt
    13741 tgcagataac tctttcatca gtgcaagcgg cacttcctca ttcgaagatt cgcggcttat
    13801 taccatatct cctccaacca ggatcatatc cggtctgcat tcatcaattc gttcgatcaa
    13861 ttcctgatta ttctctccaa actgtgcaca atgcagatct gttaaaaaca cgatccgata
    13921 tccatcaaat gcttctggaa tcttttctga ctctatttcg taatattcaa ctgtcaatct
    13981 cttattttca taactgctta aaagcactgc cacggcaatc cccacaacca acagcactgc
    14041 tatgatccat atccataaag gaatcataaa cactccttct ctctgtttcc caatccatct
    14101 tctgattcgt aaatgtcttt ttcctatttt actagatata gcaaaaaaaa acaacccata
    14161 gcacaacatc tttaggaata ttgtacaaaa aaagagaatg cccagattct gacatcctct
    14221 ttttctcatt ataatattac tgtctggaaa gcagccttat tttacgattg cgatcgtgct
    14281 tccgatatca agtgctggaa gctcggcttt ttccatgcag atagttcctg gaagacactc
    14341 agcgtcttct ccaccgaagt tatatgtaac atgtcctaaa ttcttcagat tctcgttggc
    14401 aacttctcca acttttacaa tcttgaattc tttaccgtca attacaagtt tacatccagg
    14461 agttacttct ccatctactg gatttacatc gatggaatag cagaaatctt tcagagtatc
    14521 tggtgcgtta tcaccaaatg taacgaagat cccttcttct ctaaatgcgt ctaccatagc
    14581 tccttttcct ttaatcgtta cctgatacat aatactactt tcctttctca ctaataatta
    14641 tttttatttt gctgcatata atccaaagct tgctaagtaa ccaacgataa ctcgaggtac
    14701 accagtgata aatcttgagt acagtacagc tggtacacca acttctactg tttcaggatc
    14761 tgcttctgca agtccaagtc ctactggaac gaagtcacaa gcacactgtg tattgattgc
    14821 aaataatgct ggaagtgcaa ggcttggttc aatgtttcct ttaccaattt ctacaccgat
    14881 caatgttccg acgatctgag caattacagc tccaggtcca agcagacagc ttaagaatgg
    14941 aatagaacaa ataaatccta agattacaag tccaatgata tttccacata atggctgtaa
    15001 taatttagc
//
LOCUS       DAOMUE010000020        14606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_873_length_14606_cov_3.516872, whole genome shotgun sequence.
ACCESSION   DAOMUE010000020 DAOMUE010000000
VERSION     DAOMUE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 14606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14606
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_873_length_14606_cov_3.516872"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /gene="lysS"
                     /locus_tag="PLC71_02030"
     CDS             complement(<1..1461)
                     /gene="lysS"
                     /locus_tag="PLC71_02030"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRM01458.1"
                     /translation="MAQQNKDTNELIKVRRQKLADLQAAGKNPFEIMKYDVTHHSKEI
                     KDNFEELEGKEVAVAGRLMFKRVMGKASFCNVQDLQGGIQAYVARDEIGVESYQDFKK
                     MDIGDIVGIKGKVFATKTGEKSIHAEEVILLSKSLKPLPEKFHGLTDTDTRYRQRYVD
                     LIMNEESKEVFIKRSKIISKIRSYLDGQGFMEVETPMLVSNAGGASARPFETHYNALS
                     EDVKLRISLELYLKRLIVGGLEKVYEIGRVFRNEGVDTRHNPEFTLMELYQAYTDYHG
                     MMDLTENLYRYLAEEVCGGTKIQYKDFEIDLGKPFERITMVDAVKKYSGVDFKEIKTL
                     EEARAAAEEHHVEYEERHKRGDILNLFFEEFVEDKLIQPTFVMDHPVEISPLTKRKPE
                     DPDYVERFEFFMNGWEMANAYSELNDPIDQRERFKAQEELLAQGDEEANTTDEDFLNA
                     LEIGMPPTGGIGFGIDRMVMLLTNSTAIRDVLLFPTM"
     gene            complement(1477..1956)
                     /gene="greA"
                     /locus_tag="PLC71_02035"
     CDS             complement(1477..1956)
                     /gene="greA"
                     /locus_tag="PLC71_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRM01459.1"
                     /translation="MAEAKKNILTPDGLKALEDELQELKVVRRKEIAQKIKEAREQGD
                     LSENAEYDAAKDEQRDMEARIEEIEKILKNAVVIDEKFEKGVISLGCHVLLEDITLGK
                     EVTYHIVGSTEADILQNKISNEAPIGRALLGKKIGDVVSVEGINQVNDFRVIDVQRK"
     gene            complement(2026..3024)
                     /gene="dusB"
                     /locus_tag="PLC71_02040"
     CDS             complement(2026..3024)
                     /gene="dusB"
                     /locus_tag="PLC71_02040"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRM01460.1"
                     /translation="MRSNKLKPLVIGDITIEHPLALAPMAGVTDLPYRLLCKEQGAGM
                     MCTEMVSAKGLYYGNRKSEPLMATDPKEIPVAIQIFGSDPEIMGQMAAKVNDGRFQMI
                     DINMGCPVPKIVNNGDGSALMKNPELAGKVIKAVVDAVDIPVTVKIRKGFNDELINAP
                     EMAQVAQENGAAAIAVHGRTREQYYSGTADWSIIRKVKEAVDIPVIGNGDITCAKDVL
                     RMQEETGCDGFMIGRRAKGNPWIFKEILHFFETGEEMPRPSLEEVVAMMLRHGQMMID
                     FKGEYIGVREMRKHVAWYTFGFPGAAKLREKVNHTETYEDLEELLQNYLKATNGRD"
     gene            complement(3011..3775)
                     /locus_tag="PLC71_02045"
     CDS             complement(3011..3775)
                     /locus_tag="PLC71_02045"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRM01461.1"
                     /translation="MLLALDVGNTNITIGVFKRKEIIEVFRITTKLPRTSDEFGILIS
                     SLLKEKGLDEGEVKAAIIGSVVPNIMHSLSSGLIKYFDVKPIIVGPGIKTGINLGRFN
                     PREVGADRIVDLVAGYEIYGGPALVIDYGTATTYDIVTENGVFLAGVIAPGIRTSANV
                     LYRDAARLPEIEILKPKTILANSTIDSMQAGLYYGVVGETKYMVQKMKEETGFDNMKV
                     IATGGLGNMIAQTVDEIDYYDPTLTLKGLQIIYEKQ"
     gene            complement(3779..4111)
                     /locus_tag="PLC71_02050"
     CDS             complement(3779..4111)
                     /locus_tag="PLC71_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6145 family protein"
                     /protein_id="HRM01462.1"
                     /translation="MYQDKVTLCGSNAYEKKYYLNPDFSGLPEQIKQELQIACVLFTA
                     EVGGVLTLEFDEEGKLNFVTHVEDNDFFYDEIGSELMIKKLRQEKQDFWESLELYYKI
                     FLLGEDEV"
     gene            complement(4157..5137)
                     /locus_tag="PLC71_02055"
     CDS             complement(4157..5137)
                     /locus_tag="PLC71_02055"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRM01463.1"
                     /translation="MKSKILKELKNADDYISGQQLCERLGVSRTAVWKHIKALRAEGY
                     EIDSVTNKGYKLMMEPDLLTKEEVASCLHTKWLGKDLHCYETMDSTNLEIRRLAEAGA
                     GHGTVAITEEQTMGKGRRGRSWLAEAGAGIAMSFLLKPQIEAQNSSMLTLVTALAVNE
                     AICETTGIRAKIKWPNDIIVNGKKICGILTEMSLQMDEINYIVVGLGINVNIDHFPED
                     LSDKATSLQIEGGRKIKRAPLAAKVLECFEKYYDMFLKTEDLSMLQDEYNKLLVHTDQ
                     KIKVVRGSKEEIFLSRGINHRGELLVEDEDGKVSEVSSGEVSVRGILGYV"
     gene            complement(5153..6121)
                     /gene="argF"
                     /locus_tag="PLC71_02060"
     CDS             complement(5153..6121)
                     /gene="argF"
                     /locus_tag="PLC71_02060"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRM01464.1"
                     /translation="MEEQKMDAKTSLKGRSFLTLKDFTPEEIGYFLDLAAKLKADKKK
                     GITGNSLKGKNIALLFEKPSTRTRCSFTIGCVDEGAHPEYLGKDDIQLGHKESVEDTA
                     RVLGRMFDGIEFRGFLHENVEKLAKYSGVPVWNGLTDDYHPTQILADFLTLREQFGEI
                     KGLNFVFAGDGRNNMSNSLMIGCSKMGLNFTCLAPKSLWPNEELVKQCEEYAKESGAK
                     IRFTEDVKEAVEGADCIYTDVWASMGEEDKAAERIALLRPYQVNKEMLEMTGKDTTVV
                     MHCLPAVKGHEITEDVFEHYADVIFDEAENRMHTIKAVMVATLGEY"
     gene            complement(6219..6869)
                     /locus_tag="PLC71_02065"
     CDS             complement(6219..6869)
                     /locus_tag="PLC71_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRM01465.1"
                     /translation="MKTIVCYGDSNTYGYNPENGFRYEYEERWTTILQKELKDSAIVI
                     PEGLNGRTTSFEDELRPGRNGATYLDPCLHSHGPIDLVVLMLGTNDLKIRFQATPTDI
                     GKGIDRLIKMIKSITPQKRQDGRSAKILLMAPPHLGDDLTEIPSGEEMGFERGISYSK
                     RLAPIYEDWAKFHNIEFLDAAKYAKASEIDACHLTRESHRKLGEMVAKKCKEILEI"
     gene            complement(6974..7960)
                     /locus_tag="PLC71_02070"
     CDS             complement(6974..7960)
                     /locus_tag="PLC71_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRM01466.1"
                     /translation="MIMSIILFIIVIALLAMIISSTVRIVPQAHAYVVERLGAYQGTW
                     SVGLHVKVPFIDRVARKVNLKEQVVDFPPQPVITKDNVTMQIDTVVYFQITDPKLYSY
                     GVENPIMAIENLTATTLRNVIGDLELDETLTSRETINTQMRATLDVATDPWGIKVNRV
                     ELKNIIPPAAIQDAMEKQMKAERERREAILIAEGEKKSAILRAEGQKESMVLQAEGDK
                     EAAILRAEAKKEATIREAEGQAEAIRAIQKANAQGIESIKAAKADNAVIQLKSLEAFA
                     KAADGKATKIIIPSEIQGMAGLVKSITEVAKDDEKNIADLIEEQVQDTVKEQ"
     gene            complement(7962..8393)
                     /locus_tag="PLC71_02075"
     CDS             complement(7962..8393)
                     /locus_tag="PLC71_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRM01467.1"
                     /translation="MYAVYWLIASAVFLLVEIMTLGLTSIWFAGGAVVAAIVALFGVP
                     FWVQMLIFIVVTCLLFALTRPVAKRYLNSKVQKTNTDALIGQTALVKETINNMESKGL
                     VQLNGQDWTARSFEAGEIIPEGSEVIVKEIRGVKLIVEREV"
     gene            complement(8394..9167)
                     /locus_tag="PLC71_02080"
     CDS             complement(8394..9167)
                     /locus_tag="PLC71_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRM01468.1"
                     /translation="MITTTQNKQIKQIIKLKKSARERRKQELFLVEGIRMFTEIPEEY
                     LYKVYASEDFYNEHRVIFEHMDVELVSPQVMKEISDTMTPQGVLALVKMMKYSLEDLL
                     EQEKPLFLVLENLQDPGNLGTILRTGEGAGINGVIMSHDTVDIYNPKVTRSTMGSIFR
                     VPFVYVDDLLEVAETMKQKNIITYAAHLNGTDYTKEDYQKGTAFFIGNEGNGLTDKLT
                     DKVQKKIKIPMCGKVESLNAAMASGLLVYEARRQRQGVD"
     gene            complement(9190..9837)
                     /locus_tag="PLC71_02085"
     CDS             complement(9190..9837)
                     /locus_tag="PLC71_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRM01469.1"
                     /translation="MKKSVAVLGLGKFGSSIARSLAKGGAEVLAVDKDEDLVRDIADK
                     VTCAVCVDISDKEMMNNIGLEGMDAVVIATAEHLDSGVMALMIVKEIGVPYVLAKADG
                     TLKGEILKRVGADEIVYPEEEMGARIANNLLGGSMTDLFDLSADTSIVKVPARKEWIN
                     KSLVDINFRGKYNVNVIAIEGEGGVDGAPDPTKPIKDEENLVLIGKKEDLVKLRK"
     gene            complement(9891..11243)
                     /locus_tag="PLC71_02090"
     CDS             complement(9891..11243)
                     /locus_tag="PLC71_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRM01470.1"
                     /translation="MKELRPVQVILLGFLITIFAGSILLALPIATQSGQATPYIDALF
                     TATTSVCVTGLIVETTMTHWSIFGQAVIILLVQIGGLGVITITTGMFFMLRKRITLGN
                     RMLIQESMGLNTMTGLVPLVKKILIGTGIIEGIGAVLYATQYVPEFGIGYGIWAAIFN
                     SISAFCNAGMDIVRDDSLRSYVTNPVMNFTTMGLIILGGLGFVVWKDLWQGFKKIVKD
                     KVPVKRMIQQWRFQTKIVLSITSFLILFGTILIFLFEYHNPATMESLSLPQKIQASLF
                     QSVTTRTAGFETVAQGALTDASSLVSMFLMIIGGSPTGTAGGVKTVTFAILVFCVLSV
                     AKQEESITLFKRRVPQNLLSKALAIIVINLIVLMTSVLLLLVFDHGTFMDSCYECVSA
                     LATVGLTKGLTPNLTIAGKVIIIITMYLGRVGPISMAIGFSQKNKKKMVMYPEQDLIL
                     "
     gene            complement(11315..12304)
                     /gene="pfkA"
                     /locus_tag="PLC71_02095"
     CDS             complement(11315..12304)
                     /gene="pfkA"
                     /locus_tag="PLC71_02095"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRM01471.1"
                     /translation="MGKPNIKTIGVLTSGGDAPAMNAAVRAVARIGKERGLKVKGIRK
                     GYNGLLNEDIIDLTPTETADTIFRGGTILFTARCEEFKTEEGQKRGAEICKAHGIDGL
                     VVIGGDGSFQGAKKLAALGINTIAVPGTIDLDIACTEYTIGFDTAVNTAMEAIDKIRD
                     TSTSHERCSIVEVMGRGAGYIALWCGMATGAEDILIPESFDGNLPKVEQQIIEHLLRN
                     RAKGKTHHMVINAEGVGHSYSMAKRIESATGIETRATILGHMQRGGSPTAKDRVYASM
                     MGAYAVDLLCAGKTNRVVGYQNGRFMDMDIDEGLAMTKDIDDYQLQIAHLLGK"
     gene            complement(12392..>14606)
                     /gene="dnaE"
                     /locus_tag="PLC71_02100"
     CDS             complement(12392..>14606)
                     /gene="dnaE"
                     /locus_tag="PLC71_02100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRM01472.1"
                     /translation="VRKYGKDQVVQIITFGTMAARAVIRDVGRVLDIPYAKVDGISKM
                     VPNELNITIDKALKISKDLRNAYEQDEETHYLIDMSKRLEGLPRHASMHAAGVVIGKT
                     AIDEYVPLATGADNAAVTQFTMTTIEELGLLKMDFLGLRTLTVIQDAEKMVRRKVPDF
                     DITKIDPTDKEVYEMIGNGHTEGVFQLESSGMKSFMKELKPQNMEDIIAGISLYRPGP
                     MDFIPRYIEGKNHQESIHYECEQMEEILEPTYGCIVYQEQVMQIVMKLAGYTLGRSDL
                     VRRAMSKKKGDVMIKERQNFVYGNEEEGIPGCINNGIDEKTANQIWDEMLDFAKYAFN
                     KSHAAAYAVVAYRTAYLRCHYPVEFMAALLTSVLGNTNKISEYSYACKKLGIKILPPD
                     INYGEGAFSVDHGNIRYGLTAIKSLGRPVIEAIIRERNLNGPYRDFKDLVERLSSKEI
                     NKRTVENLIKSGALDCFHVTRKQQMIVYASVIDSIQKERKNEIAGQMSLMDLLGEEDQ
                     QSFQISYPDVGEYEKEQKLAMEKEVLGIYVSGHPLEDDIERLERSVTAHTSDFVIDEE
                     TGKTKIHDRESCIIGGLISEMSVKLTKKNQNMAFITLEDLRGTVEVVVFPRQYATYQS
                     LLREDAKVFIKGTTQISEEESKVICNQIISFEDSRQELWVQFADKEAFFKEEDALKGI
                     LTSYPGKNPVVIYCKTERAVNRLGRDYMVSGDENLLFDLEKRYGKENIRVRSLGI"
BASE COUNT         3685 a         3546 c         2317 g         5058 t
ORIGIN      
        1 cattgtcggg aataataata cgtctctgat cgctgtagaa tttgttaata acattaccat
       61 tctgtcgata ccgaatccga taccacctgt aggtggcata ccgatctcta atgcatttaa
      121 gaagtcttca tctgttgtgt tggcttcttc gtcaccctgt gcaagaagtt cttcctgtgc
      181 tttgaaacgt tctctctgat cgatcggatc atttaactca gagtatgcat ttgccatctc
      241 ccatccattc ataaagaact caaaacgttc tacataatct ggatcttctg gttttctctt
      301 tgttaatgga gagatttcta ctgggtgatc cataacgaat gttggctgga tcagtttgtc
      361 ttctacgaat tcttcgaaga ataagtttaa gatatctcca cgtttgtggc gttcttcgta
      421 ttctacatgg tgttcttcag cagctgctct tgcttcttct aaagtcttaa tctctttaaa
      481 gtctactcca gagtatttct taactgcatc taccattgtg attctctcaa aaggttttcc
      541 aagatcgatc tcgaaatctt tatactgaat ctttgttcca ccgcatactt cttctgcaag
      601 gtaacgatat aagttctctg tcagatccat cattccgtga tagtctgtgt atgcctgata
      661 taattccatt aatgtaaact ctgggttatg acgtgtgtca acaccttcgt tacggaatac
      721 acgtccgatc tcgtaaactt tctcaagtcc accaacgatc agtctcttta agtataactc
      781 tagggaaata cgtaatttta catcttcact taatgcattg tagtgtgttt caaatggtct
      841 tgctgatgct ccacctgcat tggatactaa catcggagtt tctacttcca tgaatccctg
      901 tccatctaga taagaacgga tcttactgat gattttagaa cgtttgatga atacttcttt
      961 ggattcttca ttcatgatca ggtcaacata tctctgacga tatcttgtat ctgtatctgt
     1021 taatccgtgg aatttctctg gaagtggttt taaactctta gataataaaa tcacttcttc
     1081 tgcatggata gatttttctc ctgtctttgt agcaaagact ttacctttga tacctacgat
     1141 atctccgata tccatcttct tgaaatcctg ataagattct acaccgatct catctcttgc
     1201 tacatatgcc tgaattccac cctgaaggtc ctgtacgtta cagaaagatg ctttacccat
     1261 cacacgtttg aacattaaac gtcctgcaac tgctacttct tttccttcta attcttcaaa
     1321 attatctttg atttcttttg aatgatgtgt tacatcatat ttcatgatct cgaacggatt
     1381 cttcccagct gcctgtaagt cagccagttt ctgtcttctt actttgatca gttcgtttgt
     1441 atctttattc tgctgtgcca cttttcttac ttcctcttac tttctctgta catcaatgac
     1501 tctgaagtcg tttacctgat tgattccttc tacggatacg acatctccga tcttctttcc
     1561 aagtaaagct ctaccgatcg gtgcttcatt ggaaatcttg ttctgaagga tatctgcttc
     1621 tgtagaacct acgatatggt atgttacttc ttttccaaga gtgatgtctt ctaataatac
     1681 gtgacatcca agactgatga cacctttttc aaacttctca tcgataacaa cggcgttctt
     1741 taagatcttt tcgatctctt cgatacgtgc ttccatgtca cgctgttcat ctttggctgc
     1801 atcatattcc gcgttctctg ataagtcacc ctgttctctt gcttctttga tcttctgtgc
     1861 gatctcttta cgtcttacga cttttaattc ttgtaattca tcttctaatg cttttaaacc
     1921 gtctggggtt aatatattct tctttgcctc tgccataaca ctattccttt cgttctaaat
     1981 gttaactgat taattatatc ttacctaagt ttcttttgtc aattcctaat ccctgccatt
     2041 cgtggctttg agataatttt gcagtaattc ttctaagtct tcgtatgtct ctgtatgatt
     2101 taccttctcg cgaagttttg ctgcccctgg aaaaccgaat gtataccatg cgacatgttt
     2161 ccgcatctca cggactccga tgtattctcc tttaaaatcg atcatcatct gtccatgtct
     2221 caacatcatg gcgacaactt cttctaagga tggtcttggc atttcttctc ctgtctcaaa
     2281 gaaatgtaag atctctttaa aaatccatgg atttcctttg gctcttcttc cgatcataaa
     2341 tccgtcacag cctgtctctt cctgcattcg taatacatct tttgcacatg tgatatcacc
     2401 atttccaatt accggaatat caacagcttc ttttaccttg cgaatgatcg accagtctgc
     2461 tgttcctgaa tagtactgtt ctcttgttct tccatgaact gcgatcgctg ctgctccatt
     2521 ctcctgtgca acctgtgcca tctctggtgc attgatcaat tcatcgttaa atccctttcg
     2581 gatcttaact gtaaccggga tatccacagc atctacaact gctttgatca ctttccctgc
     2641 taattccgga ttcttcatca atgcggatcc atcaccgtta ttcacgatct ttggaaccgg
     2701 acatcccatg ttgatatcga tcatctgaaa ccgcccatca ttgactttcg ctgccatctg
     2761 acccatgatc tctggatcgg atccaaaaat ctggatcgct actggaattt ccttcggatc
     2821 tgttgccatc aatggctcag atttacggtt cccatagtat aatcctttgg cacttaccat
     2881 ctcagtgcac atcattccag caccctgttc tttacacaac aaacgatatg gtagatctgt
     2941 aactcctgcc atcggtgcaa gtgctaacgg gtgttcaatt gtaatatctc caataaccag
     3001 aggttttaat ttattgcttc tcataaatga tctgtaatcc cttcaatgtt aatgttggat
     3061 cgtaatagtc gatctcatca accgtctgtg cgatcatatt tccaaggcct ccggttgcga
     3121 taactttcat gttatcaaag cctgtctctt ctttcatctt ctgtaccata tattttgtct
     3181 caccaacaac tccatagtac aaacctgcct gcatactgtc aatcgtgctg tttgcaagga
     3241 tcgtctttgg ttttaagatt tcaatctctg gcagacgtgc tgcatctcta tatagtacat
     3301 ttgctgaagt gcgaattccc ggtgcgatca cacctgctaa gaatacgcca ttctctgtga
     3361 caatatcgta tgttgttgca gttccatagt caatgacgag tgctggtccg ccatatattt
     3421 catatcctgc aaccagatcc acgatcctgt ctgctccaac ttctcttgga ttaaatcttc
     3481 caagattgat ccctgtcttg atcccaggtc ctacaatgat cggttttaca tcaaaatatt
     3541 tgatcaaacc actactcagt gaatgcatga tatttggcac aacggatcct atgatcgctg
     3601 ccttcacttc accttcatcc aatccttttt cctttaatag gcttgaaatc agaattccaa
     3661 attcatcgga tgttcttggt aactttgttg taattcgaaa tacctctata atctcttttc
     3721 ttttgaatac gccgatcgtt atattggtat tcccaacatc gagtgctaat aacataattt
     3781 atacctcatc ttctcccaaa aggaatattt tataatatag ttccaaggac tcccagaaat
     3841 cctgtttttc ctgtcttagt ttcttgatca tcagttcact tccgatctca tcgtaaaaga
     3901 aatcattatc ttccacatga gtaacaaaat ttaatttgcc ttcttcatcg aactctaatg
     3961 tcaagactcc gccgacttct gctgtaaata gtacacatgc aatctgcagt tcctgtttga
     4021 tctgttctgg taatccagaa aaatcagggt ttaagtaata tttcttctca tatgcattgg
     4081 aaccgcataa tgtaacttta tcctgataca tgtttttgta tttctccttg tttcttgtat
     4141 atattctcgg aatctcttat acgtatccca agatacctct tactgatacc tctccggatg
     4201 aaacttctga tacctttcca tcttcatcct ctaccagtaa ttctccacga tgattgatgc
     4261 ctcttgataa gaatatctct tctttggaac ctctgacaac cttgatcttc tggtctgtat
     4321 gtaccagaag tttattgtat tcatcttgca acatgctaag atcttctgtc ttcaaaaaca
     4381 tatcgtaata tttctcaaaa cattctaaca ctttcgctgc caatggagct ctcttgatct
     4441 ttcttcctcc ctcgatctgg agtgaagttg ccttatcact taaatcttct ggaaaatgat
     4501 cgatatttac attgatacca agtcccacta caatgtaatt gatctcatcc atctgaaggc
     4561 tcatctctgt taggattcca cagatcttct ttccattcac aatgatatcg tttggccatt
     4621 tgatctttgc ccgaattcct gttgtctcac agattgcttc atttaccgct aatgcagtaa
     4681 ctaacgtaag catagaagaa ttctgagctt cgatctgagg ttttagaaga aagctcatcg
     4741 cgatccctgc tccagcttct gccaaccagc ttcgtcctct tcttcctttt cccatcgtct
     4801 gttcttctgt gatcgcaacg gttccgtgac cggcacctgc ctctgccaga cggcggatct
     4861 caagatttgt agaatccatt gtctcataac aatgaagatc ttttccaagc catttggtat
     4921 gaaggcagga tgctacttct tcttttgtca aaagatctgg ttccatcata agtttatagc
     4981 ctttatttgt cacagaatca atctcgtaac cttctgctct taatgctttg atatgcttcc
     5041 agacagcagt ccttgaaact cccaaccgct cacaaagctg ctgtcctgat atataatcat
     5101 ctgcattctt taattccttg agaatcttac ttttcattga atatcctatc tcctaatatt
     5161 ctcctaaagt cgctaccata acagctttga tcgtgtgcat acgattttct gcttcatcaa
     5221 aaatcacatc tgcataatgt tcaaaaacat cttctgtgat ctcatgtcct ttaactgctg
     5281 gcaggcaatg cataacaact gtagtatctt ttcctgtcat ctctaacatt tctttattta
     5341 cctgatatgg tcttaataaa gcaattcttt ctgctgcctt gtcttcttca cccatagatg
     5401 cccatacgtc tgtgtaaatg cagtctgcac cttccacagc ttctttcaca tcttctgtaa
     5461 aacggatctt agctccagat tctttcgcat actcttcgca ctgttttacc agttcttcat
     5521 ttggccataa actctttggt gctaagcatg tgaaattcaa tcccatctta ctgcatccga
     5581 tcattaaaga gttgctcatg ttatttcttc cgtctccagc gaatacgaag tttaatcctt
     5641 tgatctctcc aaactgttct cttaatgtta agaagtccgc taagatctgt gttggatggt
     5701 aatcatctgt taaaccattc catactggaa ctccactgta ttttgctaat ttctctacgt
     5761 tctcatgtaa gaatccacga aattcgatcc cgtcaaacat tcttccaaga actcttgctg
     5821 tatcttctac agattctttg tgtcctaact gaatatcatc ttttcctaga tattctgggt
     5881 gtgcaccctc atcaacacat ccgatcgtaa atgaacatct tgttcttgta cttggttttt
     5941 caaataaaag agcgatattt ttccctttta gactgtttcc tgtgatccct tttttcttat
     6001 ctgcttttaa tttcgctgct aaatctaaaa aataaccaat ttcttctggt gtaaagtctt
     6061 ttaatgttaa gaaacttctt ccctttaagc ttgtctttgc gtccatcttt tgttcctcca
     6121 ttttgttgtg tcttggatgt ctctgccctt tgatccctgt cagacatcat atagttgtta
     6181 ttatatagga atgcatcttt gaaaacaacc cgaaaccctt atatttcaag gatttccttg
     6241 cattttttcg caaccatttc acctaatttc ctatgacttt ctcttgtcag gtggcaggca
     6301 tcgatttcgg aagcttttgc atattttgct gcatccagaa attcaatgtt atgaaacttt
     6361 gcccagtctt cataaatcgg tgccaatctc ttggaataac tgatccctct ttcaaatccc
     6421 atctcttctc cacttggaat ctctgtcaga tcatccccaa gatgcggtgg tgccattaaa
     6481 aggatctttg cggatcgtcc atcttgtctc ttctgtggag tgatcgattt gatcatcttg
     6541 atcagacggt cgatcccttt tccgatatct gttggtgttg cctgaaaacg gatcttaagg
     6601 tcattcgttc caagcattaa cactaccaga tcgatcggtc cgtggctgtg taaacatgga
     6661 tctaaatatg ttgcaccatt tcttcctggt cttaactcat cttcaaaact tgttgttctg
     6721 ccgtttaatc cttccggaat cacgatcgca gaatctttca gttccttttg caaaatggtt
     6781 gtccatcgtt cttcatattc ataacggaat ccattttccg gattatagcc ataagtatta
     6841 gaatctccat aacaaacgat tgtcttcatc ttttatctcc catcaatatc aaacatggca
     6901 aaagccggaa cacctcttca gtgctccggc ataccgccaa gggttgtcat attttcataa
     6961 acattttctg atcttactgt tcttttacag tgtcttgtac ctgctcttcg atcagatctg
     7021 caatattctt ctcatcgtct ttggcaactt ctgtaattga ttttacaaga cctgccatcc
     7081 cctgaatctc agatgggata atgatctttg tagcttttcc gtcagctgct ttagcaaatg
     7141 cttctaagct ctttaactgg atgactgcat tgtcagcttt ggctgccttg attgattcaa
     7201 taccctgtgc attcgctttc tggatcgcac ggatcgcttc tgcctgacct tctgcttcac
     7261 gaatcgttgc ttctttcttc gcttccgcac gaaggattgc tgcttcctta tctccctctg
     7321 cctgaaggac catagattct ttctgtcctt ctgcacgtaa gatcgcggac ttcttctcac
     7381 cttctgcgat cagaatcgct tctcttcgtt cacgttctgc tttcatctgt ttctccatgg
     7441 catcctgaat ggctgctggt ggaatgatgt tcttaagttc cacacggttt actttgattc
     7501 cccatggatc tgtcgcaacg tcaagtgttg ctctcatctg tgtattgatc gtctctctgg
     7561 aagttaatgt ctcatctaat tccagatcac cgatgacatt tcgaagtgtt gtagctgtca
     7621 gattctcgat tgccatgatc gggttctcta caccgtaact atataatttt ggatcagtaa
     7681 tctgaaaata tacaactgta tcgatctgca tcgtaacgtt atcttttgtg ataactggct
     7741 gtggtggaaa atccacaacc tgctctttta agttgacttt tcttgcaaca cgatcaataa
     7801 atgggacttt gacatgaagt cctacagacc atgttccctg ataagcaccc agtcgttcta
     7861 caacataagc atgtgcctgc ggcacgatac gcactgtgga tgaaatgatc atcgcaagca
     7921 atgcgataac aataataaat aaaataatcg acataatcat cttatacctc tctttctacg
     7981 atcagtttca cgcctcggat ttcttttacg atcacttcgc tgccttctgg aatgatctct
     8041 ccagcctcaa agcttctcgc agtccaatcc tggccattta actgaaccaa ccctttgctt
     8101 tccatgttat tgatcgtttc tttgacaagt gctgtctgtc cgatcaatgc atcggtattc
     8161 gttttttgca ccttgctgtt taaatatcgt ttggcaactg gtcttgtcag tgcaaacaac
     8221 agacatgtta ccacgataaa tatcaacatc tgtacccaaa atggaacacc aaatagtgct
     8281 acgatcgctg caacaacggc tccacctgca aaccagatac ttgtaagtcc aagtgtcatg
     8341 atctcaacaa gtaaaaatac cgcggatgcg atcagccaat aaactgcata catttaatcc
     8401 accccctgtc tctggcgtct tgcctcataa actaaaaggc ctgatgccat ggctgcattt
     8461 aatgattcta ccttaccaca cattggaatc ttgatcttct tctgtacttt gtctgtcaac
     8521 ttatctgtca gaccatttcc ctcattgcca atgaaaaatg cggttccttt ttgataatct
     8581 tccttcgtat agtctgttcc attgagatgt gctgcatacg tgatgatatt cttttgtttc
     8641 atcgtttctg cgacttctaa aagatcatcg acatagacaa atggcacacg aaatattgat
     8701 cccatcgtgg agcgcgtaac ctttggatta taaatatcta ctgtatcatg actcatgata
     8761 acaccattga tccctgcacc ctctcctgtt cgaaggatcg tcccgagatt ccccggatcc
     8821 tgaagattct caagcaccag aaataatggt ttttcctgtt ctagaagatc ttctaaagaa
     8881 tatttcatca tcttcaccag tgcaagaact ccctgtggtg tcatcgtatc ggaaatctct
     8941 ttcataactt gaggtgatac caattccaca tccatatgtt caaaaatcac tctatgttcg
     9001 ttataaaaat cttcagatgc atagacttta tataaatatt cttctgggat ctctgtaaac
     9061 atacgaattc cctctacaag aaataattct tgttttcttc tttctcttgc actcttcttt
     9121 aatttgatga tctgtttgat ctgcttattc tgagtggttg tgatcatatt cttatccacc
     9181 tccgatctat tattttctta atttgaccag atcttctttc ttaccgatca gcacaagatt
     9241 ttcctcatct ttgatcggtt tcgttggatc tggagccccg tcaacaccac cttctccttc
     9301 gatcgcgatg acgttaacgt tatattttcc acggaaatta atgtctacta atgatttgtt
     9361 gatccattct tttcttgctg gtactttaac gatacttgtg tctgcagaaa gatcaaacag
     9421 atctgtcata cttcctccaa gcagattgtt tgcgatcctt gctcccatct cttcttctgg
     9481 ataaacgatc tcatcggctc caacacgttt taatatctct ccttttaaag ttccgtctgc
     9541 cttcgcaaga acatatggca caccgatctc tttgacgatc atcaatgcca taacaccgga
     9601 atctaaatgc tctgcagttg cgatcacaac tgcatccatt ccttccaatc cgatgttgtt
     9661 catcatttct ttatcagata tatccacaca gactgcacaa gttactttat ctgcgatatc
     9721 tcttacaaga tcctcatctt tatcaactgc cagtacttct gctccacctt tggcaagtga
     9781 ccttgcgata ctgcttccaa actttccaag tcctaaaact gcaactgact tcttcattct
     9841 tctccgttac ctccgttgcg atatcatgtt ataatgtgaa tctatttcac ttataagatc
     9901 agatcctgtt ccggatacat gaccatcttt ttcttattct tctgtgaaaa accgatcgcc
     9961 atagaaatcg gtccgacacg ccctaagtac atggtaatga tgataatcac tttccctgcg
    10021 attgtcaaat tcggtgtcaa tcctttggtt aaaccaactg tcgcgagtgc tgatacacat
    10081 tcatagcatg aatccataaa agtcccgtga tcaaatacca acagcaataa cacagatgtc
    10141 atcagcacga tcaggttgat cacaatgatc gcaagtgcct tcgacaacag attctgtgga
    10201 actcttcgtt taaataacgt aattgattct tcttgttttg ccacagagag cacacaaaat
    10261 acaagaattg cgaatgtcac tgtcttgaca ccgcctgcag taccagttgg tgatcctccg
    10321 atgatcatca gaaacataga aaccagcgaa gatgcatccg tcaatgcgcc ctgtgctacc
    10381 gtctcaaacc ctgcggtacg tgtcgtcaca gactgaaaca gtgatgcctg tatcttctgt
    10441 ggcaatgaca gactctccat agttgctgga ttatgatatt caaacagaaa gatcaatatt
    10501 gtaccaaaca agatcaaaaa tgacgtaatg gataacacga tcttggtctg aaatctccat
    10561 tgttgtatca tcctttttac aggaacttta tcttttacga tctttttgaa tccctgccaa
    10621 agatctttcc atacaacaaa tccaagacct cccaggatga tcagccccat tgtcgtaaaa
    10681 ttcataactg gattcgttac atagcttcgc agactgtcgt ctcttacaat atccattcct
    10741 gcattacaaa atgccgagat tgaattaaag attgctgccc atattccata tccaatgcca
    10801 aattccggca catactgagt cgcataaaga actgctccaa ttccttctat gatccctgtc
    10861 ccgatcagaa tcttcttaac taatggaact aaacctgtca ttgtattaag acccatagac
    10921 tcctggatca acattctatt tccaagagtg atcctctttc gaagcataaa aaacattccg
    10981 gttgtgatcg taatgacacc aagtcctccg atctgtacaa gcaatatgat cactgcctgc
    11041 ccaaatatac tccaatgtgt cattgttgtc tcaacgatca aacccgtcac acacacagat
    11101 gttgttgctg taaataatgc atcaatataa ggcgttgcct gcccgctttg tgttgcgatc
    11161 ggcagtgcaa gcagtatcga tcctgcaaat attgtaatta aaaatccaag caatatgacc
    11221 tgtaccggtc gtaactcttt cataaaataa tgctcctata tagagaaata gaggaatcct
    11281 agataaacag gattcctcta agatcatgtt ttatttattt tcctaataaa tgtgcgatct
    11341 gaagctgata atcatcgata tcttttgtca ttgcgagtcc ttcgtcaatg tccatatcca
    11401 tgaatcttcc gttctggtat ccaacaacac ggtttgtctt tcctgcacat aataagtcaa
    11461 ctgcgtaagc acccatcata gaagcgtata cacggtcttt tgctgttggg cttccaccac
    11521 gctgcatatg tccaaggatc gtagcacgtg tttcaatacc tgttgcagat tcgattctct
    11581 tcgccatact gtaagaatgt ccaacacctt cggcattgat taccatatga tgtgtcttac
    11641 cttttgctct gttacgaagt aaatgttcga tgatctgctg ttctacctta ggaaggtttc
    11701 catcaaagct ttctgggatc aggatatctt ctgctcctgt tgccatacca caccataatg
    11761 cgatatatcc agctccacgt cccataacct ctacgatact acatctttca tgagatgttg
    11821 atgtatcacg gatcttgtcg atcgcttcca tcgctgtgtt aacagctgta tcgaatccga
    11881 tcgtatattc tgtacatgcg atatctaagt cgatcgttcc tggaacagcg atcgtgttga
    11941 ttccaagcgc tgctaacttc ttagctccct ggaaagaacc gtctccacca ataactacca
    12001 atccgtcgat tccatgtgct ttacagatct cagcaccacg tttctgacct tcttctgtct
    12061 taaattcttc acatctggct gtgaaaagga ttgtacctcc acggaagatc gtatcggctg
    12121 tttctgttgg tgttaaatcg atgatgtctt cgtttaacag tccattgtaa cctttacgga
    12181 ttcccttaac ttttaatcct ctttctttac caattctggc tacggcacgc actgctgcgt
    12241 tcatagcagg tgcatctcca ccacttgtta aaactccaat tgttttaatg tttggttttc
    12301 ccatttgcaa tatcctccaa ttattaatcc atacaattct taacttggct tttattctaa
    12361 tccatttaaa gaaatttttc aatagtcttt gttatattcc taacgatcta acccgaatat
    12421 tttcttttcc atatcgtttt tcgagatcaa ataataaatt ctcgtcacca gaaaccatat
    12481 aatctctccc tagacggttc acagcacgct ctgttttgca atagatcacc actggatttt
    12541 taccgggata ggaagttaaa atacctttta atgcatcctc ttctttaaaa aatgcttctt
    12601 tatccgcaaa ttgtacccat aattcctgtc tgctatcctc aaaactgatg atctgattac
    12661 atatgacttt gctttcttct tcagaaatct gtgttgttcc tttgataaat actttggcat
    12721 cttctcttaa aagagactga tatgtcgcat actgtcttgg aaatacgaca acttccacgg
    12781 ttcctcttaa gtcttccagt gtgataaatg ccatattctg attcttcttc gtaagtttca
    12841 cactcatctc actgatcaaa ccgccaatga tacagctttc ccgatcgtga atctttgtct
    12901 ttcctgtttc ttcatcgatc acaaaatcag aagtatgtgc tgttacactt cgttcaaggc
    12961 gttctatatc atcctctaaa ggatgtccac tgacgtagat cccaagcact tccttttcca
    13021 tggcaagttt ctgctctttc tcatattccc caacatctgg ataggaaatc tgaaaagact
    13081 gctggtcttc ttctccaaga agatccatca gactcatctg tcctgcgatc tcatttttcc
    13141 gttctttctg gatactatcg atcacactgg cgtagacgat catctgctgc tttcttgtta
    13201 catgaaaaca atctaaagct cccgatttga tcagattttc tacagttcgt ttattgatct
    13261 ctttgcttga taatcgttcc acgagatctt taaaatctct gtatggtcca tttaaatttc
    13321 gttctcttat gatcgcttca atgactggcc gtcccagact cttgatcgca gtcagtccat
    13381 agcggatatt tccatggtct acagagaacg caccctctcc atagttaata tctggtggca
    13441 aaatcttaat tcctaatttc ttgcaggcat aactgtattc tgaaatcttg tttgtatttc
    13501 caagaacaga ggttaacaag gctgccataa attctactgg ataatggcat cttaaatagg
    13561 ctgtacgata ggcaactact gcatatgctg ccgcatgaga tttgttaaat gcatattttg
    13621 caaagtctaa catctcatcc cagatctggt tcgctgtctt ttcatcaata ccgttattga
    13681 tacatcctgg aattccttct tcttcatttc cataaacgaa attctgtcgt tctttgatca
    13741 tgacatcgcc ttttttcttg gacatcgcac gacgtacaag gtcacttcgt ccaagcgtat
    13801 atccggctag tttcataacg atctgcataa cctgttcctg ataaacgata catccatagg
    13861 ttggctccag aatctcttcc atctgttcac attcataatg tatactttcc tgatgattct
    13921 ttccctcaat gtatcgtgga ataaagtcca ttggtcccgg tcgatacaaa gaaattccag
    13981 caatgatatc ttccatattc tgtggtttta gttctttcat aaatgacttc attcctgaac
    14041 tttccaactg aaacacacct tctgtatgac catttccgat catctcataa acttctttgt
    14101 ctgtcggatc aattttcgtg atatcaaaat ccggcacttt tcttcgcacc attttctctg
    14161 catcctgaat gactgttaat gtacgaagtc ccaaaaagtc catcttcaac agaccaagtt
    14221 cttcaatcgt tgtcatcgta aactgagtaa cagctgcgtt atctgcacct gttgctaatg
    14281 gcacatattc atcaatcgct gtcttaccga tcacgactcc agctgcatgc atggaagcat
    14341 gtcttggaag tccttctagt ctcttcgaca tatcgatcag ataatgtgtt tcttcgtcct
    14401 gctcataagc atttctcaag tccttgctga tctttaatgc tttatcgatc gtaatattta
    14461 attcatttgg aaccatcttt gatattccat ctacttttgc atatggaata tccaaaactc
    14521 ttccgacatc acggatcacg gctcttgcag ccatagttcc aaaagtgatg atctgtacta
    14581 cttgatcttt cccgtatttt cttaca
//
LOCUS       DAOMUE010000021        14271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_916_length_14271_cov_2.531303, whole genome shotgun sequence.
ACCESSION   DAOMUE010000021 DAOMUE010000000
VERSION     DAOMUE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 14271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14271
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_916_length_14271_cov_2.531303"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..771
                     /locus_tag="PLC71_02105"
     CDS             166..771
                     /locus_tag="PLC71_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM01473.1"
                     /translation="MLDKSLVYKDIVMCLPFEDLEDLKVPVLPDGYSYKMFESGDEIA
                     WAKLEVLVGEFDCFEDASAYFAKTFLTHEELLADRVCFIVNPEGEIVATTSSWFKTNS
                     DIRFPLIHWVSTSPKEQGKGLGKAIVLFALSRFLVVEPDADFIFLHTHTWAYKAVGMY
                     QKMGFRITKKALPNSRTDFSCLDVLKDVLPNSIIAQLLEKD"
     assembly_gap    1051..1060
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1160..2323)
                     /locus_tag="PLC71_02110"
     CDS             complement(1160..2323)
                     /locus_tag="PLC71_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRM01474.1"
                     /translation="MTNLLISLFIKDKENVKDAKVRNAYGKMAGIVGICCNIFLFATK
                     FFAGIITGAISITADAFNNLSDAASSIITLVGFGMAGKPADEDHPFGHGRMEYVSGLV
                     VSILILMMGVELFKTSVEKVIHPEVITEQWISYVILVISIALKFWMYMFNKGIGWRIH
                     SETMKATAADSLNDCISTAAVVGGMLVFHYFHLNIDGIVGIFVACFVLWGGYESIKDT
                     MNPLLGESPDPELIAKITETVLNHKMVIGVHDIVVHDYGPGRRIVSLHAEVPYNKDMM
                     EVHDLMDHIEMRLMEEYHCEATIHMDPVVTDDEEVNETRKEVFNIVKKYDKTLSVHDF
                     RMVKGPTHTNVIFDLVIPYGKDYEPEKIVKDLKQKIKDEMQGKHYAVIRVDRF"
     gene            complement(2418..3659)
                     /locus_tag="PLC71_02115"
     CDS             complement(2418..3659)
                     /locus_tag="PLC71_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRM01475.1"
                     /translation="MGQTMIEKIISRNVGVDSVKPGQIQTVNVDRVMIHDIFIPFVAE
                     KFEEMGFTKLWDPDKVVLIYDHMVPASTTDDIRHFKIGDAFAKKYGMKNVHRSDGICH
                     QLMTECGYAKPGNIVFGTDSHTTTYGCVGCFSSGIGYTEMAAILGTGEMWVRVPETIK
                     VVIDGKLPENVSSKDIILRLIGDLTAAGATYKALEFSGSTVDEMSVAARMTMSNMAIE
                     AGAKAALFAPDEKTAEYSKVDLADVDWLYGDEDAEYCQTITYKAEDLVPVVACPSQVD
                     KIRAVKEVEGTELDQVFIGSCTNGRLEDLKAAAEILKGKKVADYVKLIVTPASRKIYK
                     QAVDAGYMKILAEAGAIITHPGCGLCCGRAGGIMTDGERVLATNNRNFLGRMGTSKVE
                     IYLGSPKTAAASAIAGKIVEA"
     gene            complement(3664..4155)
                     /locus_tag="PLC71_02120"
     CDS             complement(3664..4155)
                     /locus_tag="PLC71_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HRM01476.1"
                     /translation="MEQFKGKVWVLGDDIDTDIIIPTEYLALPTVEDMKQYGFSPLRP
                     ELAGQIGEGDIIVAGSNFGCGSSREQAPEIIKALKVKCVIAKSFARIFFRNSINNGLL
                     LIENDKIQDAVKEGDEIEVTLGEKITYNGTDYEIHSLPDNLMEIINAGGLVEAMKKRN
                     GVK"
     gene            complement(4182..5426)
                     /locus_tag="PLC71_02125"
     CDS             complement(4182..5426)
                     /locus_tag="PLC71_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRM01477.1"
                     /translation="MGMTIGEKIIARAAKVDQVKAGEIHTIEVDRLMSNDGTTHLTID
                     MYHNQLKHPKIADKDKLVFIMDHNVPAENPKTAAAHRKMRNFAKEHEIKLYEGKGVCH
                     QIMVENHVCPGEFIMGADSHTCTYGALGAFGTGIGCTDFLYAMVTGQSWVLVPDTIRF
                     NLHGKLREGVYARDLMLSIIGMVGANGCNYKIMEFAGEGAHNLDIDERLVLCNLAVEA
                     GAKTGIVEPDEKVVEYVESRGRKAENLFKSDDDAVFEKVYDINLDEIKPVVARPHQID
                     DVVDAKEVKDVKIDEAFLGSCNNGRIEELRVAAEILKGKKVSDSVRFLIAPASNEVYM
                     QALDEGLIDIFMESGAMVMNCNCSVCWGSCQGVIGKDEVLISTGTRNFKGRAGHPDSY
                     VYLGSAATVTASAIAGKITTAD"
     gene            complement(5431..5907)
                     /gene="leuD"
                     /locus_tag="PLC71_02130"
     CDS             complement(5431..5907)
                     /gene="leuD"
                     /locus_tag="PLC71_02130"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204192.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRM01478.1"
                     /translation="MGRVFKFHNDVDTDQIIASQYLLLPNIEEMKVYTFESIDKDFAK
                     KVKEGDIVVAGENFGCGSSREQAPSVLKALGVKAVVAKSFARIFYRNSINIGLPVIIS
                     KDLYDKVEDGADIDINLSEGWIKTEDETLPCTKLPPYMQNILDHGGLINFLNEGEE"
     gene            complement(6019..6459)
                     /locus_tag="PLC71_02135"
     CDS             complement(6019..6459)
                     /locus_tag="PLC71_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HRM01479.1"
                     /translation="MRQIANNVYIPVLKDLVEEGKEVSMMISGSSMNPFLIHQRDYIL
                     MKKPEEELKAGDMVFFQRRDEAYVMHRIHHINKEGKLFIIGDAQVDMEGPIDKEQVFA
                     IITKVKRKGKWIAPGDFWWEFFEHIWLHLIPFRRFLMKLYGIRR"
     gene            complement(6456..6725)
                     /locus_tag="PLC71_02140"
     CDS             complement(6456..6725)
                     /locus_tag="PLC71_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRM01480.1"
                     /translation="MKRNTDFMLRDIAGEVILVPTGAATQQFNGMITLNEVAAFIWKN
                     LDESKSKEELVDKIMDEFEVDEETARTDVEGFVGALYEHGLVLDE"
     gene            complement(6722..8206)
                     /locus_tag="PLC71_02145"
     CDS             complement(6722..8206)
                     /locus_tag="PLC71_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRM01481.1"
                     /translation="MNFSMIRYVIGLVMLFESAFLSLPCLIALIYHEKKGFSFWIMLF
                     VCLIIGILFVMKKPKKTVYYAKEGFLTVAISWIVMSFFGALPFVINGDIPSVVDAMFE
                     TVSGFTTTGSSILTDVEALARCSLFWRSFTHWVGGMGVFVFVLAVMPLVGGQNIHLMR
                     AESPGPSVGKLVPKIRKTSMILYKIYIFMTIVMVVLLLLGKLPLFDSLLLAFGTAGTG
                     GFSILNSGCASYSPYIQYLIAIFMILFGVNFNVYYFILIKKFKDAIHYEELKYYLLFI
                     GASVAMITYNIHSLFPTIEQAFRHALFQVGTVITTTGYASTDFNKWPEFSKFILVMLM
                     FSGACAGSTGGGMKVSRLVIMLKTVKKELLSYLHPRTVRKVKFNGRIVEHEVIRGINV
                     YMIAYAFVLMFSVLIISIDNFDFTSNFTAVVATLNNIGPGLNIVGPTGNFSMYSNLSK
                     IVMIFDMLAGRLELFPMLLLINPYTWKEIKIRKNKKNKKEGHSI"
     gene            complement(8222..9583)
                     /gene="trkA"
                     /locus_tag="PLC71_02150"
     CDS             complement(8222..9583)
                     /gene="trkA"
                     /locus_tag="PLC71_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRM01482.1"
                     /translation="MNIIIVGCGRIGSTLVEELSGENHDISIIDEKAHVVQKLAETHD
                     VLGVIGNGASYSILSDAGVETADIIIAVTNSDELNLLCCLIAKKAGRCETVARVRNPV
                     YYDEISFIKEEMGISVVLNPELSAAYEIARLLRFPSAMEVNKFSKGRIEMLKYRIPHN
                     SKLDGMTLIDVSNQLNCEILISAVERKKELIIPNGSFQLREKDIISFVGRPNCAMEFF
                     RKIGERKNQVHEAMIIGGSKIGFYLSKLLVSSGIGVKLIDKDRDQCERLNESIPDAMI
                     IHGNGNDHDMLLEEGLTDVDAFIALTNHDEENMFLSLYAQSQSDAKLVTKVQKLNYDA
                     MIERLDLGSMIYPEYLTAQYIIQYVRARQNSIGSNIENLYKLMDGRAEALEFHIQKGS
                     PVIGRPIKDLLLIDNLLICSIYRKGKPMIPNGQDHMEEDDLVVVVTTKKGLHDVKDIL
                     KRG"
     gene            complement(9795..10349)
                     /gene="hxlB"
                     /locus_tag="PLC71_02155"
     CDS             complement(9795..10349)
                     /gene="hxlB"
                     /locus_tag="PLC71_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HRM01483.1"
                     /translation="MKVKENLEHILKELQDATFKIEEEQIENVLKLIAPDKKIFLTGK
                     GRSGLAAKGFANRLMHLGFQAYVIGEISTPHTKAGDLLIITSGSGETDALVSIAKKAK
                     ESGLYLGLVTMNPQSTLGKMADGMIILPGDSKGNNEEKHSIQPMGSQFEQMSFLIFDA
                     IVLKLMENWNQTSEQMFMRHADLE"
     gene            complement(10346..11002)
                     /gene="rpe"
                     /locus_tag="PLC71_02160"
     CDS             complement(10346..11002)
                     /gene="rpe"
                     /locus_tag="PLC71_02160"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRM01484.1"
                     /translation="MKIISPSLLNSDTYQIKEQFEALEKCGINYIHIDITDGHFVPMI
                     SFGANTVKDLRKHTDFVLDCHLMVENPENLIPVIADAGADIITVHTEATKHIYRSIQT
                     IKKCGKKAGIAINPGTPVSMIKEILPMADLVLCMTTNPGVFGESFIPSVADKVKNLCE
                     LREQKGYSYQIQVDGSINDQTAIVCKKVGADIFVSGSYIFGGNIEERIHKIMDAGEEM
                     "
     gene            complement(11005..11520)
                     /locus_tag="PLC71_02165"
     CDS             complement(11005..11520)
                     /locus_tag="PLC71_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HRM01485.1"
                     /translation="MGVFFGEVTLETVKGRASYHDISDEIRENIKKSELKNGCVVISS
                     AHTTCSLYFDECMHDTNYWGDEYLQADINDVMEKIAPSMKMENQYHSPGPKHIEFGLS
                     LGSKDYPAEKWTMLNTDAHLKSSIFGSPSLTFIVKDGEIQLGALGKVYFVDWDQLRER
                     TRKVQIMVMGE"
     gene            complement(11530..11814)
                     /locus_tag="PLC71_02170"
     CDS             complement(11530..11814)
                     /locus_tag="PLC71_02170"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM01486.1"
                     /translation="MKKLLIVCGAGHATSTIAVAKVSAWLEKEGYSDKVKIYQSKIAD
                     ELNKIDDYDAVISTTIVPDSIKDKVIQGLPLLNGMGVDQVYDQLKTRLGL"
     gene            complement(11854..>12825)
                     /locus_tag="PLC71_02175"
     CDS             complement(11854..>12825)
                     /locus_tag="PLC71_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HRM01487.1"
                     /translation="VDIWNFWFPCFLGLVAQAVTGNFVTGILGALVAVMLQWLLADIF
                     QKKVSEFFGYPGIAISHMMALSGALIAVPLNWIFDRIPGFNKIEADSETIAKRFGFFG
                     DTVVIGIIIGMIVGFLAKYDFAKAAQLGMATGAVMKIMPKMVAMFMEGLMPIAEAAKE
                     FANKRLGGRSVNIGMDAALTVGHSTVMSTNLLMVPISLALAIILPGNQTLPFGDLAFY
                     AFGICLMIPIFRGNVVRSIVGCSIYMVSMLYLSTWLAPIITNVFKIAHYNVGTSGQVT
                     SVLCGIWPAGLFAVATKSLGNIGLAVIGVVVIALLVYVNKIRAQKEN"
     assembly_gap    12826..12843
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12844..13187)
                     /locus_tag="PLC71_02180"
     CDS             complement(<12844..13187)
                     /locus_tag="PLC71_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HRM01488.1"
                     /translation="MLSVLQWFIGLGSTVFLPIIIFILGIIFGVKPAKAAISGFTVGI
                     GSIGLNLVIGLLSDNLGTAIQVMGEHYGFSLNIMDIGCGVGGPLAFSTTFGIILIPMS
                     LIVNLLFVMLGWT"
     assembly_gap    13261..13270
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13271..13649)
                     /locus_tag="PLC71_02185"
     CDS             complement(<13271..13649)
                     /locus_tag="PLC71_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM01489.1"
                     /translation="MYFDQRLCFFHQKVESQDQLFQMMTDKMLEAGCVKENYYEGISN
                     REKEYPTGLLVNQTGFAIPHTDSERVNKSQICFLSLDEPIEFEDMVDKSHKISVELVF
                     MLAMAQPHEQVNTLQNLIALFQDD"
     gene            complement(13668..>14271)
                     /locus_tag="PLC71_02190"
     CDS             complement(13668..>14271)
                     /locus_tag="PLC71_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM01490.1"
                     /translation="DLIISSVDIEDPEISYVKVNPLLTKDDYANILDAYTKQVLLIKN
                     NVCDNQKNGIKAPTLKKYLEGKFIFLKQDLDSKEKCLDFIIDVLEKDNAVYDEFREAI
                     YKREKLGVTCLDTGVALPHADPQTIKKSRIILLTLKHPVDWGGTLVSLIVVTAFPEEE
                     MNQIRDVINELYQLIGEKEDVNTFIRFETIQEVLKVFHES"
BASE COUNT         4065 a         3163 c         2090 g         4915 t
ORIGIN      
        1 aaacggaagg gtgtgtatgt tagacgttat tggttttcaa tttttgtagt gattcgtaga
       61 aagtactttc tatttaattt atactctgtg acttttcatt caatagcttg tatactctaa
      121 ttaatcaatt tattgttatt tttaatttta tgggggtttt tgtttatgct tgataagtct
      181 cttgtttata aagatattgt gatgtgtctg ccttttgagg atttagagga tcttaaggtt
      241 cctgttttgc ctgatggata ttcttataag atgtttgaat ctggggatga gattgcttgg
      301 gcgaagcttg aagtgttggt tggagagttt gattgttttg aggatgcttc tgcttatttt
      361 gctaaaactt ttttaactca tgaagaactt ttagctgacc gggtttgttt tattgtgaat
      421 cctgagggag agattgttgc tacgacgagt tcttggttta agacaaatag cgatattcgg
      481 tttcctttga tccattgggt gtctacttct cctaaagaac agggaaaggg gcttgggaag
      541 gcaattgttt tgtttgcttt gtctcgtttt cttgttgtag agccagatgc tgattttatt
      601 tttcttcaca cgcatacttg ggcttataag gctgttggta tgtatcagaa gatgggcttt
      661 cgtattacga aaaaagccct gccaaatagc aggactgatt tttcttgtct ggatgttttg
      721 aaggatgtac ttcctaattc gattattgct cagcttttag agaaagatta agatgctgtc
      781 cagtcatatt gatcttatat atactaacac acacttatat ttttaatcat cttaattgac
      841 ataaaaatat ataaatgtta ctataataat agaaaaggtg ctaccgatga cggtttgcct
      901 gatataaacg attgaataac cgctcacata acttgtcagg tcaatgggcg gttatttctc
      961 tatcttggca tcatgctttc cacgcacata accaagacta taaaaagcca cacacagcga
     1021 aatactgcaa tttgacaaat cggtgcttgt nnnnnnnnnn atttgacatc cctaggttta
     1081 tatttcaaat attttaacaa atcggtgctt gtttaagccc tttcatcttc tgtcacatac
     1141 gcgtaagcct acctacaaat cagaatctgt ctacacgaat cactgcataa tgtttcccct
     1201 gcatctcgtc tttaatcttc tgcttaagat ccttcacgat cttctctggc tcataatctt
     1261 tcccataagg aatcacaaga tcaaagatca cattcgtatg tgttggccct ttgaccattc
     1321 gaaagtcatg aactgacagt gtcttgtcat atttcttaac aatattaaaa acttcttttc
     1381 ttgtctcatt cacttcctca tcatccgtca ctacaggatc catatggatt gttgcttcgc
     1441 agtgatattc ttccatcaaa cgcatttcaa tatgatccat cagatcatgt acttccatca
     1501 tgtctttatt gtatggaact tctgcatgca aagagacaat tcttcttcct ggaccatagt
     1561 cgtgaacaac gatatcatga actccgatga ccatcttatg atttaataca gtctctgtga
     1621 tcttagcgat caattctgga tctggggatt ctcctaatag ggggttcatg gtatctttga
     1681 ttgattcata accaccccag agtacaaaac atgcaacaaa gattccgaca attccatcaa
     1741 tatttaaatg aaaataatgg aatacaagca ttcctccaac aacagctgct gttgaaatac
     1801 aatcatttaa actatccgct gctgtcgctt tcatcgtttc agaatgaatt ctccatccga
     1861 ttcctttgtt aaacatatac atccaaaatt ttaatgcgat cgagatcact aatattacat
     1921 aagaaatcca ttgttctgtg atcacctctg gatgaatcac tttttctact gatgttttaa
     1981 ataattccac tcccatcatc aagatcagga tagatacaac aagtcctgat acatattcca
     2041 tccttccatg tccgaatgga tgatcttcat ctgctggttt tccagccatt ccaaatccga
     2101 caagtgtgat aatagaagat gctgcgtcag ataaattgtt aaacgcatct gctgtgatag
     2161 agattgctcc tgtgatgatt cctgcgaaaa atttggttgc aaataagaag atgttacaac
     2221 agattcctac aatccctgcc atctttccat aagcatttct tacttttgca tcctttacat
     2281 tttctttgtc tttaataaat aatgatatta ataagtttgt cataaaaaac tccatctaca
     2341 tagtaaatgg gagtcctaat gacccccaat gtactttttc tttatattta tgaggatctt
     2401 tacctcatat gttcacttta tgcttctacg atcttacctg cgattgctga tgcagctgct
     2461 gtcttaggtg atcctaagta gatttctact tttgacgttc ccattcttcc taagaagtta
     2521 cggttgtttg ttgcaagaac acgttctcca tctgtcatga tcccgcctgc acgtccacaa
     2581 cataatccac atcctggatg cgtgatgatc gctccggctt ctgccaaaat cttcatgtat
     2641 ccagcatcca ctgcctgttt ataaatcttt ctactagctg gtgttacgat caattttaca
     2701 taatcagcaa ctttctttcc ttttaagatc tctgctgccg ctttcagatc ttctaatctt
     2761 ccgtttgtac aagatccgat aaatacctga tcaagctctg ttccttcgac ttctttgaca
     2821 gctctgatct tatctacctg tgatggacat gccactactg gtactaaatc ttctgcttta
     2881 tatgtgattg tctggcaata ctcagcatct tcatctccat ataaccaatc aacatcagct
     2941 aaatctacct tagaatattc agctgtcttt tcatccggtg cgaaaagtgc tgctttcgct
     3001 cctgcttcga ttgccatatt tgacattgtc attctggctg caacactcat ctcatcaact
     3061 gtgcttccag agaattctaa tgctttataa gtagctccag ctgctgttaa atctccgatc
     3121 aatcgtaaga taatatcttt agaacttaca ttttcaggaa gttttccatc aatgactact
     3181 ttgatcgtct ctggaactct tacccacatc tctcctgttc caaggatcgc tgccatctct
     3241 gtataaccga ttcctgaaga gaaacatcca acacaaccat aagttgttgt atgagagtct
     3301 gttccgaata cgatattccc aggttttgca tatccacatt ctgtcattaa ctgatggcag
     3361 atcccatcac ttctgtgaac atttttcatg ccatatttct tggcaaatgc atcaccgatc
     3421 ttgaaatgtc tgatatcgtc tgttgtactt gctggtacca tgtggtcata gatcaatacg
     3481 actttgtctg ggtcccataa ctttgtaaat cccatttctt cgaatttctc tgctacgaat
     3541 ggaataaaga tatcatgaat cataacacgg tctacattta ctgtctggat ctgtcctggt
     3601 tttacactgt ccacaccaac gtttctgctg atgatctttt caatcattgt ctgtcccata
     3661 tctctatttc accccatttc ttttcttcat ggcttctact aatccaccgg cgttgatgat
     3721 ctccatcaag ttatctggaa gggaatggat ctcataatct gtaccattat atgtgatctt
     3781 ctctcctaaa gtaacttcaa tctcatcgcc ttctttaact gcatcctgaa tcttgtcatt
     3841 ctcaattaac agtaatccat tattgatgga attacgaaag aaaatacgtg caaatgattt
     3901 ggcaatgacg catttaactt ttaatgcttt gatgatctct ggtgcctgtt ctcttgaaga
     3961 accgcatcca aagttgcttc ctgctacgat aatatctcct tcaccgatct gtcctgctag
     4021 ttctggacga agtggagaaa atccatactg tttcatatct tctacggttg gaagtgccag
     4081 atattctgtt gggatgatga tatctgtatc aatatcatct ccaagcaccc aaacctttcc
     4141 tttaaactgt tccatatcta ttctatcctt ctctcttaaa tctagtctgc tgttgtgatc
     4201 ttacctgcga tcgctgatgc tgtgaccgtc gctgctgatc ctaaatatac ataagaatcc
     4261 ggatgtccgg cacgtccttt gaagtttctt gttcctgtag aaatcaaaac ttcatctttc
     4321 ccgatgacac cctgacagct tccccagcaa acgctgcagt tacagttcat aaccattgct
     4381 ccggattcca tgaaaatatc gatcagacct tcatctaatg cctgcatata aacttcattg
     4441 gaagctggtg cgatcaaaaa tcttacgctg tcacttactt tcttaccttt taagatttca
     4501 gcagcgactc ttaattcttc gatacgtcca ttgttacatg atcctaagaa tgcttcatcg
     4561 attttcacat ccttgacttc tttggcatcc acaacatcat cgatctgatg aggtcttgca
     4621 acaactggtt tgatctcgtc taagttgata tcataaactt tctcaaatac cgcatcatca
     4681 tcgctcttga ataagttctc agcttttctt ccacgacttt ctacgtattc tacaactttc
     4741 tcatctggct caacgattcc agtcttagct cctgcttcaa cagcaaggtt gcaaagaact
     4801 aaacgttcat caatatcaag gttatgagca ccttctccag caaattccat gatcttgtag
     4861 ttacatccat ttgctccaac cattccgatg atagaaagca ttaaatctct ggcgtataca
     4921 ccttctctta atttaccatg caggttaaaa cggatcgtgt ctggaactaa tacccaagac
     4981 tgtcctgtta ccatagcgta taagaaatca gtacatccga ttcctgtacc aaatgctcct
     5041 aaagctccat atgtacacgt atgggagtct gcacccatga taaattctcc tggacataca
     5101 tgattctcta ccatgatctg atggcagaca ccttttcctt catataattt gatctcatgc
     5161 tcttttgcaa aattacgcat ctttctatgt gctgctgctg tttttggatt ctccgcaggc
     5221 acattgtggt ccatgataaa taccagtttg tctttgtccg cgatctttgg atgttttaac
     5281 tggttatgat acatatcaat tgttaaatgt gtcgttccgt cattactcat taaacggtca
     5341 acttcaatcg tatggatctc tcctgctttt acctgatcta ctttcgcagc tcttgcaata
     5401 atcttttctc cgatggtcat tcccatgttc ctattcctcc ccttcattca agaaattgat
     5461 caatccgcca tgatctaaga tattctgcat atatggtggc agcttggtac atggaagggt
     5521 ttcatcctct gttttgatcc atccttcaga caggttgata tcaatgtcag ctccgtcttc
     5581 cactttgtca tataagtctt tgctgatgat cactggtaat ccgatattga tagagtttct
     5641 atagaagatt ctcgcaaatg atttagcaac cactgctttg acacctaacg ctttcagtac
     5701 acttggcgcc tgttctctgg atgacccaca tccaaagttc tctcctgcta caacaatatc
     5761 tccttctttt accttcttgg caaaatcttt atcaattgat tcaaaagtat atactttcat
     5821 ctcttcaata tttggcaata acaaatattg tgacgcgatg atctgatctg tatctacgtc
     5881 gttatggaat ttaaatacac gtcccattga tcttcctcct gttttgtatc ttttattttt
     5941 aaggatacta taaattggaa aaaaaatcta tgtctgataa caaaaaaaga atggtaatac
     6001 gtaaaatatt accacccttt atcttcgaat tccgtataat ttcatcaaaa atctccgaaa
     6061 tggaatcaaa tgcagccaga tatgttcaaa aaactcccac caaaaatctc caggtgcgat
     6121 ccattttcct ttacgcttta cttttgtaat gatcgcaaaa acctgttctt tgtcaatcgg
     6181 tccttccata tctacctgtg catctccgat aataaacagt tttccctcct tgttgatgtg
     6241 atgaatccga tgcatcacat acgcctcatc tcttctctga aaaaacacca tatccccagc
     6301 tttcaattct tcttctggct tcttcatcaa aatatagtct ctttgatgga tcaaaaacgg
     6361 attcatactg ctcccactga tcatcatgct gacttctttt ccctcttcta ccagatcttt
     6421 caataccggg atataaacgt tatttgcaat ctgtctcatt catccaatac cagtccatgt
     6481 tcataaagtg ctccgacaaa tccttctaca tctgtccttg ctgtttcttc atctacttca
     6541 aattcatcca tgatcttatc aaccaattct tcttttgact tcgattcatc taaattcttc
     6601 caaataaatg ctgccacttc atttaatgtg atcattccat taaactgctg agttgcagcc
     6661 cctgttggta caagaattac ttcacctgcg atatcacgaa gcataaaatc tgtattacgt
     6721 ttcatatgct atggccctct ttcttatttt ttttattttt tcttattttt atttctttcc
     6781 atgtatatgg attgatcaac aataacattg gaaataattc cagtcttccc gctaacatat
     6841 caaagatcat aacgatcttg gaaagattcg aatacattga aaagtttcca gttggtccta
     6901 cgatattaag cccaggacca atattattca atgttgcaac aactgccgta aaattgcttg
     6961 taaaatcgaa gttatcaata gaaatgatca gtacagaaaa catcagtaca aatgcatatg
     7021 cgatcatata aacattgatc cctcgaataa cttcatgttc tacaattctt ccgttgaatt
     7081 tgaccttacg taccgttctt ggatgtaagt atgagagcag ctctttcttg acggtcttta
     7141 acataatgac caatcttgat actttcatac caccaccggt acttccggca catgctcctg
     7201 aaaacatcag cattaccaga ataaactttg aaaattccgg ccatttatta aaatctgttg
     7261 atgcatatcc ggttgtcgtg atcactgttc ctacttgaaa aagtgcatgg cggaatgcct
     7321 gctcgatcgt tggaaataaa ctgtgtatat tatatgtgat cattgctaca gatgcaccaa
     7381 taaataataa gtaatatttt aattcttcat aatggatcgc atctttgaac ttcttgatca
     7441 aaataaaata atatacatta aaatttactc caaataagat cataaagatc gcgatcaaat
     7501 attgtatgta tggtgagtaa gatgcacacc ctgaattcaa tatcgagaat ccgccagttc
     7561 cagcagtacc gaatgcaagt aacagcgaat caaataatgg aagctttcct aaaagcaaca
     7621 atactaccat cacaatagtc ataaaaatat aaatcttata caagatcata gatgtttttc
     7681 gaatcttagg aaccagtttt ccaacagatg gtcctggact ttctgctctc atcagatgaa
     7741 tattctgacc accaaccaat ggcatgacag ctaatacaaa cacgaataca cccataccac
     7801 ctacccagtg agtaaaactc ctccagaata aactacatct tgctaatgct tctacatccg
     7861 ttaagatact tgatcctgtt gtagtaaatc ctgaaaccgt ttcaaacatc gcatctacaa
     7921 ccgatggaat atctccattg atcacaaatg gaagtgctcc aaaaaaactc atcacaatcc
     7981 aactgatcgc aactgttaaa aaaccttctt ttgcataata aactgtcttc tttggtttct
     8041 tcataacaaa aagaattccg atgatcagac atacaaatag catgatccag aaagaaaaac
     8101 ctttcttctc atgatagatt aatgcgatca gacaaggaag tgataaaaat gcactctcaa
     8161 agagcattac caagcctatt acgtatcgaa tcattgaaaa attcattata ccctgccccc
     8221 attatcctct ctttaagatg tctttgacat cgtgtaatcc ttttttagtt gtgacaacta
     8281 ccaccagatc atcttcttcc atatgatctt gaccatttgg aatcattggt tttcctttac
     8341 gataaatgct gcagatcagc agattatcaa tcaataacag atctttaatt ggtcttccaa
     8401 tgactggaga acctttctga atatgaaatt ccagtgcttc cgctcttcca tccattaatt
     8461 tgtagagatt ctcaatatta cttccgatgg aattttgtct tgctcttaca tactgaataa
     8521 tatattgtgc tgtcagatat tccggataga tcatacttcc aagatctaat cgttcgatca
     8581 tggcatcgta attcaatttc tgtacctttg tgaccaattt tgcatcactc tggctctgtg
     8641 catacagaga cagaaacata ttctcctcat cgtgattcgt aagggcaata aatgcatcta
     8701 catccgtcag accttcttct aacagcatat catggtcatt tccatttcca tggatgatca
     8761 tcgcatctgg aatactctca tttaatctct cacattgatc tctgtcttta tcgatcagtt
     8821 ttacaccaat tccactagaa acaagtaatt ttgataaata aaatccaatc ttacttccac
     8881 ctatgatcat tgcttcatga acctgattct ttctttcccc aatcttcctg aaaaattcca
     8941 ttgcacagtt tggccttcca acaaatgaaa ttatatcttt ctctcttaac tgaaaagaac
     9001 catttggaat gatcaattct ttcttacgct ctaccgcaga gatcaatatc tcacaattta
     9061 actgattact gacatcgatc aatgtcattc catctaattt agaattatgt gggatacggt
     9121 attttaacat ctcgatcctg cctttagaaa acttattcac ttccattgca gaagggaatc
     9181 ttagtaatct tgcaatctca tatgccgcac ttaattctgg attcagcaca acagaaattc
     9241 ccatctcttc ctttatgaaa ctgatctcat cataataaac aggatttctc actctggcaa
     9301 ccgtctcaca tcttcctgcc ttcttcgcga tcagacaaca caaaagattc aattcgtcag
     9361 agttcgtaac tgcaataatg atatctgctg tctcaactcc tgcgtctgac agaatactat
     9421 aacttgctcc attcccgatc acacctaata catcatgtgt ttcagcaagt ttctgtacaa
     9481 catgtgcttt ctcatcaata atggaaatgt catggttctc gccacttaat tcctcaacaa
     9541 gcgtagaacc gattctacca cacccaacaa tgataatatt cattttttac cctcaattca
     9601 ttaacacttt cttaacaaat ttttatactt tttttaatac ttgtcctatt ctatcacagt
     9661 ctgtcagaaa ttacaattta agaaatatcc ttttaattat gacatctaaa aatatcctgt
     9721 aagaccaaac ataatttatc tgatcttaca ggatattttc ttcccacaca tcatctttca
     9781 acgcatggat atcattattc taaatctgca tgccgcataa acatctgctc cgatgtctga
     9841 ttccagtttt ccataagttt taatacgatc gcatcgaaga ttaaaaatga catctgttca
     9901 aactgacttc ccattggctg tatcgaatgt ttctcttcat tatttccttt ggagtcacct
     9961 ggaagtatga tcatcccatc tgccattttc cccagtgtgg attgcggatt cattgtaaca
    10021 agtccaagat atagtccaga ttctttggct ttttttgcaa tactgaccaa ggcatctgtt
    10081 tctcctgacc cagatgttat gatcaataga tctccagctt tcgtatgtgg tgttgaaatt
    10141 tctccaatga catacgcctg aaatccaaga tgcatcaatc tgtttgcaaa acccttcgcc
    10201 gccaaccctg atcttccttt tccagttaaa aatatctttt tgtctggtgc tatcagtttt
    10261 aaaacatttt caatctgttc ttcttctatt ttaaaagttg cgtcttgaag ttcctttaaa
    10321 atatgttcta aattttcttt tactttcaca tctcttctcc tgcatccata atcttgtgaa
    10381 ttcgttcttc gatatttcct ccaaatatat agcttcctga aacaaaaata tctgcgccta
    10441 cctttttaca cacaatggct gtttgatcat taatgcttcc atctacctga atctgataag
    10501 aatatccttt ttgttctctc aattcacaaa gattctttac tttgtctgca actgatggaa
    10561 taaaactttc tccaaacaca ccgggattag tggtcatgca aaggacaaga tcagccattg
    10621 gcaaaatctc cttgatcata gataccggag ttccgggatt gatcgcaatt ccagcttttt
    10681 ttccacactt tttgattgtc tgaatagatc ggtagatatg cttcgttgcc tctgtatgca
    10741 cggtgatgat atctgccccc gcatccgcaa tcactggtat cagattttct ggattttcaa
    10801 ccatcagatg acaatccagt acaaaatcag tatgttttct cagatctttt actgtatttg
    10861 cgccaaacga gatcattggt acaaaatgtc catctgtaat atcaatatga atgtagttta
    10921 ttccacattt ttctaatgct tcaaactgct ccttaatctg atatgtatct gaatttaata
    10981 atgaaggact gataattttc atttttattc tcccatcacc ataatctgta cttttcttgt
    11041 tcgttctctt aactgatccc agtctacaaa atagactttt cccaaagctc caagttggat
    11101 ttctccgtct tttacgataa atgttaatga cgggctgcca aaaatggaag atttcagatg
    11161 tgcatctgta ttaagcattg tccatttttc tgctggataa tccttagatc caagacttaa
    11221 tccaaattca atatgttttg gtcctggact atgatactga ttttccattt tcatggatgg
    11281 cgcaattttt tccatcacat cattaatatc tgcctgcaga tattcatctc cccagtaatt
    11341 tgtgtcatgc atacattcgt caaaatacag agaacatgtg gtatgcgcac ttgatatgac
    11401 aacacatcca ttttttaact cagatttttt gatattttct ctgatttcat cagaaatgtc
    11461 atgataagat gctcttcctt taactgtttc taatgtcact tctccaaaaa aaactcccat
    11521 gatctgctcc tataatccaa gtcttgtttt taactgatcg tatacctgat ctactcccat
    11581 tccgtttaat aatggtaatc cctgaatgac tttatctttg atagaatctg gcacgatcgt
    11641 tgttgatatc accgcatcat aatcatcaat tttatttaat tcgtcggcaa tcttagactg
    11701 ataaatcttt actttatcgc tgtatccttc tttttctaac catgcgctga cttttgctac
    11761 cgcaattgtg gatgttgcat gtcctgctcc acatacaatt aataattttt tcatgatact
    11821 acctcctatg aaatatctta ttttataaag attctaattt tctttctgtg ctctgatttt
    11881 atttacatat actagtaatg cgatcacgac aactccaatc actgctaatc caatatttcc
    11941 taatgatttt gttgcaactg cgaataatcc cgctggccag ataccgcaaa gtacggatgt
    12001 tacctgtcca cttgttccaa cattataatg tgcgatctta aatacatttg taatgattgg
    12061 tgcaagccat gtagaaagat acagcataga taccatataa atactacatc ctacaataga
    12121 tctcacaaca tttcctctaa aaattgggat cattagacag attccaaatg catagaatgc
    12181 aagatctccg aatggtaagg tctggtttcc tggtaagatg atcgctaatg ctaaagaaat
    12241 tggtaccatt aataagtttg tactcataac tgtagaatgt ccaacagtta gagcagcatc
    12301 cataccaata tttacacttc ttccacctaa tctcttattg gcgaattctt tggcagcttc
    12361 tgcaattggc attaaccctt ccataaacat tgctaccatc tttggcatga ttttcataac
    12421 cgcacctgtt gccattccta actgtgcagc ttttgcgaaa tcatattttg ccaagaatcc
    12481 tacgatcatt ccaatgatga ttccgataac aactgtatct ccaaaaaatc caaaacgttt
    12541 tgcgatcgtt tctgagtcgg cttcaatttt attaaatccc ggaattctgt caaaaatcca
    12601 gtttaatggt actgcgatca atgctcctga taacgccatc atatgagaaa ttgcaattcc
    12661 tggatatcca aaaaattcac taactttctt ctggaaaatg tctgctaata accactgaag
    12721 cataactgca actagtgctc ctaaaattcc tgtaacaaaa tttcctgtaa ctgcctgtgc
    12781 aactaatcca aggaaacatg gaaaccagaa gttccagata tctacnnnnn nnnnnnnnnn
    12841 nnngtccacc caagcatgac aaataataaa ttaacgatca atgacattgg gatcaatata
    12901 attccaaatg ttgttgagaa tgccagcggt ccaccgacac cacaaccaat atccataata
    12961 tttagtgaaa atccgtaatg ttctcccatt acctgtattg ccgttccaag gttatctgat
    13021 aataatccaa tgaccagatt cagaccaata ctaccaattc caactgtaaa acctgaaatc
    13081 gctgcttttg ctggttttac tccaaaaata atacctaata tgaaaataat gatcggtaaa
    13141 aataccgttg accctaatcc gataaaccat tgtaatacac ttaacatttc ttcttctctc
    13201 tttctctatc gcttattcaa ttcctgatgc atttaaaatc tgcataagtt ctttctctgt
    13261 nnnnnnnnnn catcatcctg aaacagtgcg atcaaattct gtaatgtatt tacctgctca
    13321 tgtggctgtg ccattgctaa cataaatacc aattccactg aaatcttatg acttttatcc
    13381 accatatctt caaattcaat tggctcatct aatgataaaa agcaaatctg cgatttattc
    13441 actcgctcag agtctgtatg cggaatcgca aagcctgtct gatttactaa taatcctgta
    13501 ggatattctt tttctctgtt actgattcct tcatagtaat tttcttttac acatcctgct
    13561 tctaacattt tatctgtcat catctgaaac aactgatcct gactttccac tttttgatga
    13621 aagaaacaca atctctggtc aaaatacatt caagttctcc tttcatatca tgattcatga
    13681 aatactttta aaacttcttg tattgtttcg aatcttataa acgtatttac atcttctttt
    13741 tctccaataa gctgatagag ttcattgatc acatcccgaa tctggttcat ttcttcttct
    13801 ggaaatgcag ttacaacgat cagtgatact aaagttccac cccagtcaac gggatgtttt
    13861 aaagttaata ggattattct ggatttttta atcgtctgcg gatctgcatg tggcagtgcc
    13921 acacctgtat ccaaacatgt cacaccaagt ttttctcttt tgtaaatagc ttccctaaat
    13981 tcatcataca ctgcattatc tttttctaat acatcaataa taaaatctag acacttttct
    14041 ttactatcaa gatcttgttt aaggaaaata aatttccctt ccaaatattt tttcaatgtc
    14101 ggtgctttaa ttccattttt ctggttatca cacacattat ttttgatcag taatacctgt
    14161 tttgtatatg catctaggat attggcataa tcatcttttg taagcagcgg atttaccttc
    14221 acataagaaa tctctggatc ttcaatatca acagatgata tgatcagatc t
//
LOCUS       DAOMUE010000022        14015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_954_length_14015_cov_2.549499, whole genome shotgun sequence.
ACCESSION   DAOMUE010000022 DAOMUE010000000
VERSION     DAOMUE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 14015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14015
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_954_length_14015_cov_2.549499"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1668)
                     /locus_tag="PLC71_02195"
     CDS             complement(262..1668)
                     /locus_tag="PLC71_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRM01491.1"
                     /translation="MKNVKKMIQAGLKKFTVITILGVFLMTSLIPVSAATKVSKIKWS
                     AYRKTMYVGNAQRFAVKITPAKASKAKLGWKTSNKKIAKVSAKGVVTPVKAGKATITC
                     YVKSQKSKKVTCKVTVKKQKVKAITFAKASVAVQKGKKVSNPAIVTPTYAANKKVTYK
                     SSSTSVATVSTSGVVTGKKVGTATITATAADGSKKKNSYKVTVVTPITKNSAKFIAHR
                     GLSAEAPENTIKAYELAGGAEFWGAETDVRMTKDKKFILQHDLTFKRLCGVDKKPEDM
                     TLSEIQKLTIKSGNNISKYKNVKSATTVATLEDYLTTCKKYNMVPVIEIKMEFVEYGN
                     ETTNDSRMQAVTKNNMEDLYALTNQIMGNKEYMFIAYDFETMVQMRKVLDDNATTSTN
                     VKLQHVTNNPDQGMINYYKKRKIELDANCDKISLSDIKAFKDGGVNVGLWTVDDTERV
                     AEYIAQKVDYITTNTKFW"
     gene            complement(1760..4486)
                     /locus_tag="PLC71_02200"
     CDS             complement(1760..4486)
                     /locus_tag="PLC71_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRM01492.1"
                     /translation="MIKEQRKYCYGVYTLMFLLMCIVAFLPFFTEGKSFVWGAGVEDG
                     LSQHFSALAYYGEALREFFRNLLAGHPKLVMWDMSLGYGADILSTLNYYAIGDPLNLL
                     YGFVSPKNTETMYDFMILLRMYLAGITFIMYARKMKKRSYGTVIGALVYVFSGFCFRL
                     GLRHPFFINPMIYFPLLCLGIEKIYQRERPYVFIFAVCVSAMSNYYFLYMLTIFAVIY
                     AWIRFYKYTEENKMKNFFLTILKFGIYYTLGIAMAAVILLPSVIGFLGNGRYGNGVDW
                     KSLIVYPGKYYLLFIENFIGYGNMGSNTNAGYLPIVGIVVLFTLFSQRMKHKKYRAAF
                     IASIIALILPIFGYAFNGFSYANNRWAFALSFIVALLTAEMYPRLFVMSKRQQIGIGA
                     GIIIYTVFCIIVNASGEEILKNKGIMAACGLIAVFYILLLIFQRLGYDTQKRIVRVSM
                     AILLLISVGVHGYYRFDPKGYAYTQEFMDQGQAYRTLKEDNIRMLSKVNDPSVYRVHA
                     EGYRYKNYGLINHLNTISGYYSITAKCVTDTIKGYDTLGMQYADKYKGVDQRLGLLSL
                     AGVKYITVAHNSQVAKDVSSKGNVPYGVEKQSKKGNITLYKNKYALPFAYAYDSYMTE
                     QQYEQLNGIGKEQAMLAQIILNQHPADKEIQHNEQRNDPDIQTISLPETRISSPKGKK
                     YADITVPVEKDKETYLYFKNLVYHGKKNGDDNFILTGRKGTKGILVTQNDVQQKIHIQ
                     STFNPYYFGRKDYIVKINHQTSKAKEKVRLNFLSPGEYEFDDISLITVPKKDVLARLK
                     ERKENSMKQIQYEGNHFRGVYHAKKDQILCVTIPYSKGWKATVNGNRTKIYKANGMFM
                     GIIMKKGIQSVKLDYETPGLKIGAWISLVAWIGLGIYGLYFEKYRKNLLNQR"
     gene            4634..5050
                     /locus_tag="PLC71_02205"
     CDS             4634..5050
                     /locus_tag="PLC71_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRM01493.1"
                     /translation="MLNLIKKLYNDSRIRYLFFGGLTTLVNLGMFALLKGPCGIDYKI
                     SNFFSVAIAICFAFVVNKLYVFQSKSESFTDTIHEFIKFVLGRLVTMAVEVGGVPFCV
                     EILHQPQMIAKLETQVIVTVVNYFISKFLVFKSQKK"
     gene            complement(5118..6047)
                     /locus_tag="PLC71_02210"
     CDS             complement(5118..6047)
                     /locus_tag="PLC71_02210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM01494.1"
                     /translation="MDKMLSVVLPAYNEEQMIKKTAQVVGDLLEKEQIPYELVFVNDG
                     SKDHTWEKILELKEERAHIKGVCFSRNFGKEGAVFAGITQAEGDCIAVMDCDLQHPPE
                     TLIKMYRLWEDGYQVIEGVKASRGRESFIHKMFAKTFYSIISDATGIDMSRASDFKLM
                     DRQAAEEFLKLPERNVFFRALSSWVGFKTTYVEFDVQEREAGESKWSFKSLVRYAVNN
                     ITSFSAAPMQIVTFFGVVFFVFAVLLGIQSLYMYFSGHAVAGFTTVILLLLLVGSILM
                     FSLGVIGYYIAKIYDEVKMRPRYIISEVIKSKE"
     gene            complement(6070..7293)
                     /locus_tag="PLC71_02215"
     CDS             complement(6070..7293)
                     /locus_tag="PLC71_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRM01495.1"
                     /translation="MKKRMESDLLLKIISVVFAFLLWMFVINTDNPVIKKTFSDVPVD
                     MLNEQVLDDLNQTYKIESGDTVSFTVKGKKDVVDRLTKSDFRATADVSSMSDVHAIQI
                     EVEALRYKSQLDIDTGGATVKVVLEDVKSDQIPVNVVVKGTPASGYTVSTQTATPNLI
                     SVSGPKSVVSRIKQIVAKVDVSGLKKDVTMAQNVKCYDEDGDEVSQDRIKLDTTKIKV
                     KIGLSRTKTIPFTVETKGTPGKGYVLGSIDYEPKHIEITGAEDDLEKISSIKLANLDI
                     SDSTKSIEKTIKASDIKLPDGITFVKSVDKIKNIVIRANIEKKTKRTLTIPTEQIALI
                     NNTKNYDVKFDDSELKVKISGLRSVVDKVVVKDLNPKIDMQLYEPGTHTVQVQLTKIK
                     NMSIEKNVSAKITVE"
     gene            complement(7271..>7901)
                     /gene="cdaA"
                     /locus_tag="PLC71_02220"
     CDS             complement(7271..>7901)
                     /gene="cdaA"
                     /locus_tag="PLC71_02220"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRM01496.1"
                     /translation="ITAILIVFQPELRSALEQLGRKNWVTDIFSTDSRQEELEYTART
                     LQEISRAAIEMGKVKTGALIVLQREVALGEYERTGIPIDAKVSSQLLINIFEHNTPLH
                     DGAVIIHNNRIVSATCYLPLSDSVEIGKEMGTRHRAAVGISEVSDSITVIVSEETGGI
                     SIARDGKIIRHLDPQRLRDELAVLREEKGQNKKFKLWKGRNRNEKKNGK"
     assembly_gap    7902..7911
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7912..8159)
                     /locus_tag="PLC71_02225"
     CDS             complement(<7912..8159)
                     /locus_tag="PLC71_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HRM01497.1"
                     /translation="MSKFFDGLEKYIYWTSIPKIGLNDIVDILIVSVMIYLIVKWIKT
                     TRAWVLLKGIIILLCIAFAAYLLQLHTVSWILSNTMGVG"
     gene            complement(8156..9154)
                     /locus_tag="PLC71_02230"
     CDS             complement(8156..9154)
                     /locus_tag="PLC71_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01498.1"
                     /translation="MEKLLIYRERFLKFLTARGRYIQSGMRFLGGTVLFYVLGELFGY
                     TETFSQPFFIFMMGVISVFIPISALSLIFYVVIFLELLHVSLEVTLFFALVVVLYFLV
                     YQRVFPETRIYLMMVPIFFYFQLPACLPIFVGMFCGIAGLPAILMGTVIYYLSNILQQ
                     TMNQLASGSAHGKVYSLIAARAIDNKDLLLYFVVFCLVTALVTAIRKRGAAHGWNISI
                     LAGGVVYLICMFIGGYLVNNEINITSQIGTIIGSIAIALIIQFLYNVIDYTREETFEF
                     EDEEYYYYVRAIPKVTVEEEEFNVTQITVPSHRFSLKRKEHKQEHKEQDKEKGEEI"
     gene            complement(9132..9587)
                     /locus_tag="PLC71_02235"
     CDS             complement(9132..9587)
                     /locus_tag="PLC71_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01499.1"
                     /translation="MISEEKVKIMAEMAKYEKNYGKGDFRIYKYTKKDYVRSEKIKTS
                     IALLISFLIMTGLLCIMQLDIVIMKLREGKVIVSAAVIIGIYVAVFFVYLHFTKIRAS
                     RQYDEVRARVLIYDKHLEELETLYKEKENEDISPTIVEEEDEDGKIIDI"
     gene            complement(9687..10232)
                     /locus_tag="PLC71_02240"
     CDS             complement(9687..10232)
                     /locus_tag="PLC71_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HRM01500.1"
                     /translation="MKKWICTVCGYVHEGEQPPEKCPQCGVPADKFKEQVEGEKEWAA
                     EHVVGVAKGVSEDILADLRANFEGECSEVGMYLAMARVAHREGYPEIGLYWEKAAYEE
                     AEHAAKFAELLGEVVTDSTKKNLEMRVDAEHGATEGKFDLAKRAKAANLDAIHDTVHE
                     MARDEARHGKAFEGLLKRYFG"
     gene            complement(10366..10761)
                     /locus_tag="PLC71_02245"
     CDS             complement(10366..10761)
                     /locus_tag="PLC71_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRM01501.1"
                     /translation="MQPATKKSKQRDAIIKFLMTRKDHPTADMVYMNIKEEFPKISLG
                     TVYRNLALLSERGEILKLSYEGGADHYDACTDPHYHFVCQECGAVIDLEMAPIDEKLN
                     EIASENFPGKITQNIVYFKGICKNCLKNH"
     gene            complement(10950..12347)
                     /locus_tag="PLC71_02250"
     CDS             complement(10950..12347)
                     /locus_tag="PLC71_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRM01502.1"
                     /translation="MSYKKFDQADLEVIKNIIKDDERILYAENINEEYSHDELGGAQS
                     YPDVVVKATSAEEISEIMKYAYENNIPVTPRGAGTGLVGSSVAIEHGIMIDSSLMNHI
                     LELDEENLTITVEPGVLLMELAAYVEDHDFFYPPDPGEKSATIGGNISTNAGGMRAVK
                     YGVTRDYVRGLEVVLPNGKIVEFGGKVVKNSSGYSLKDLMIGSEGTLGFITKATLKLL
                     PLPKKAISLLIPFKTLKEAIDTVPLIIKSKAIPTAIEFMQREVIIDAEEYLGKKFPDS
                     QSDAYLLLKFDGNSTEEIEADYDKVAKLCLEAGALDVLISDTEEREESIWKARGAFLE
                     AIKGSTTDMDDMVVPRNKVNEMVEYLHNLHNEVDIRIKSFGHAGDGNLHSYILKDDLS
                     QEEFEKRMAEAMEKIYEKAAQLGGKVSGEHGIGFAKKPYLRESLDPETVELMSGIKKA
                     FDPKNILNPHKVFQS"
     gene            complement(12353..>13244)
                     /locus_tag="PLC71_02255"
     CDS             complement(12353..>13244)
                     /locus_tag="PLC71_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRM01503.1"
                     /translation="INHPVYALFMGNNISDKAEELLHYGVDKVFVYDFPELARFKIES
                     YTSVFEDFIKKHQPCAILTGATTVGRQLAPRVAARMKTGLTADCTILEMDENTDLSQI
                     RPAFGGNIMAHILTPNNRPQMATVRYKVMNAPERTEETHGEIISCAIDKEKIKAHVDV
                     LDIVKKEPEKFIESADVLVVAGRGIKKEEDLAMIRELAELLDGQVACTRPLAEAGWVE
                     AKCQVGLSGRTVRPKLIITCGVSGAIQFVAGMDHSDCIVSINSDEKAPIFKTAHYCLV
                     GDVYDIVPKLIEKIKAAKGA"
     assembly_gap    13245..13254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13255..13556)
                     /locus_tag="PLC71_02260"
     CDS             complement(<13255..13556)
                     /locus_tag="PLC71_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HRM01504.1"
                     /translation="MPKLVIHQEKVEDPQVLVDICPFGAMEEKDGKLSINAACKMCKL
                     CVKKGPAGAVEYVEDEKKEEIDKSQWNGIAVYVDHVDGEIHPVTYELIGKARELAS"
     gene            complement(13572..>14015)
                     /locus_tag="PLC71_02265"
     CDS             complement(13572..>14015)
                     /locus_tag="PLC71_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HRM01505.1"
                     /translation="CGKQTTDGDTAQVGSEVSEWLNIPSVSNVKKISKVESDAITIEM
                     DLPEELEIAKVQYPCLLSVDKDIFQPRLPSYVKKMDTADREIEVYSLDDMEDKDETHY
                     GLNGSPTQVKRIFPPTANDHHEVWDGDGSEVADKLFHMITDMKFI"
BASE COUNT         3578 a         3112 c         2224 g         5081 t
ORIGIN      
        1 tactacattc ctgtaacttt tcttttcatc ttactttctt ctctggtatt cttttgctgt
       61 cttcacgatc tgaaagactc tttctgtgtc cattttgctt tcgcagagca ttctgatccg
      121 ttctcctagc catgtaacta atttatctct tcttgatgtt gtgatagatg gtgatcgttt
      181 taattatacc acaaaccata acaccaaaaa agagacaaca aacatcttca tctgctgtct
      241 cttttcttgt gttttctatt cttaccagaa ctttgtattc gttgtaatat aatccacttt
      301 ctgtgcaata tactctgcga ctctttctgt atcgtccaca gtccaaagtc ctacattcac
      361 tccaccatct ttgaatgcct tgatgtctga gagtgaaatc ttatcacaat ttgcatccaa
      421 ttcaatctta cgtttcttgt aataattgat cattccctga tctggattgt ttgttacatg
      481 ctgtaatttt acatttgtgg atgttgttgc gttatcgtct aagacttttc tcatctgtac
      541 cattgtttca aagtcatagg caataaacat gtattcttta ttccccatga tctgatttgt
      601 taatgcatac agatcttcca tgttgttctt tgtcactgcc tgcattcgag agtcattggt
      661 tgtttcattt ccgtattcta cgaattccat cttgatctcg ataactggta ccatgttgta
      721 cttcttgcat gttgttaaat aatcttctaa tgttgcaact gtcgttgctg attttacatt
      781 cttatatttt gaaatgttat ttccagattt gatcgtcagt ttctggatct ctgataatgt
      841 catatcttct ggtttcttat caactccgca taatctcttg aatgtcaggt cgtgctgcag
      901 aatgaatttc ttgtcttttg tcatacggac atctgtttct gctccccaga attctgctcc
      961 acccgctaat tcataggctt tgatcgtatt ctctggtgct tcggcactta atccgcggtg
     1021 tgcgataaac ttggcactat tttttgtgat cggtgttaca acagtcactt tgtaactgtt
     1081 tttcttcttt gatccatcgg ctgctgttgc tgtgatcgta gctgttccga ctttctttcc
     1141 tgttacaact ccacttgtgc tgactgttgc cacgcttgtg ctagaagatt tgtatgttac
     1201 ctttttattt gctgcgtaag ttggtgttac gatcgctgga ttgctgacct ttttcccttt
     1261 ctgtacggct acgcttgctt ttgcaaacgt gatcgctttc actttctgtt ttttcactgt
     1321 gactttgcat gttactttct ttgatttttg tgatttcacg taacatgtga tcgttgcttt
     1381 tcctgctttg acaggtgtga caactccttt tgcactgact ttggcaatct tcttattact
     1441 tgttttccat ccaagcttcg ctttggatgc tttggctggt gtgatcttca ctgcaaatct
     1501 ctgtgcattt ccaacataca ttgtctttct atatgctgac catttgatct ttgatacttt
     1561 cgttgctgcg gatactggaa tcagacttgt cataagaaat acacctaaga tagtgatcac
     1621 tgtgaatttc tttaatcctg cctgtatcat tttttttacg tttttcatag ttcctcccac
     1681 tcatttaaca tttttgtaac atttgtctaa gggctatttt atcatgtttt ttgtattttt
     1741 accagtatct cttcttattt taacgttgat ttaatagatt ttttctgtac ttttcaaagt
     1801 acaatccata tattcccagt ccgatccatg ccaccagact gatccatgcc cctattttta
     1861 atcctggtgt ttcataatct aatttgacac tttgtattcc ttttttcatt atgatcccca
     1921 taaacatccc gtttgcctta taaatctttg tgcgatttcc attgactgtt gctttccatc
     1981 cttttgagta tggaatcgtt acacacagaa tctggtcttt tttcgcatga tagactcctc
     2041 tgaaatgatt tccttcgtat tggatctgtt tcattgagtt ctccttgcgt tcctttaatc
     2101 tggcaagaac atctttttta ggaactgtga tcaaactgat atcatcaaat tcatattcac
     2161 ctggagacaa gaaatttaat cttacttttt cttttgcctt gcttgtctga tgattgatct
     2221 ttacaatgta atcttttctt ccaaaatagt atggattgaa ggtgctttga atatgaattt
     2281 tctgttgtac atcgttttgt gtcacaagga ttccttttgt tccctttctt ccagtcagta
     2341 tgaagttatc gtctccattt ttctttccat ggtaaacgag atttttaaaa tacagataag
     2401 tttccttgtc tttctctact ggcaccgtaa tatccgcata tttcttccct tttggagatg
     2461 agatccttgt ctctggcaat gagatcgtct gtatatcagg atcgtttctt tgttcgttgt
     2521 gttgaatctc tttgtctgcc ggatgctgat tcaatatgat ctgtgcaagc attgcctgtt
     2581 ccttgccgat tccatttaac tgctcgtatt gctgctctgt catataagaa tcatatgcat
     2641 atgcaaatgg cagtgcatat ttattcttat ataatgtgat atttcctttt ttactttgtt
     2701 tctcaactcc gtatggtaca ttccccttgg aacttacatc ttttgcaacc tgactgttat
     2761 gtgctactgt tatgtatttt acgccagcta atgacaggag tccaagtctt tgatcgactc
     2821 ctttatattt atctgcatac tgcattccca acgtatcata tcctttgatc gtatctgtca
     2881 cacattttgc tgtgatactg taatatccgc tgatcgtatt caaatggttg atcagtccat
     2941 aatttttata tcgatatccc tctgcatgta ctcgataaac agatggatca tttaccttac
     3001 tcagcatacg gatattatct tccttcaatg ttcgatatgc ttgtccctga tccataaatt
     3061 cctgtgtata agcatatcct tttggatcga atctgtaata tccatgcact cccactgata
     3121 tcaggagcaa gattgccatg ctcactcgca caattctctt ctgtgtatca tagccaagtc
     3181 tttgaaagat caacaataga atataaaata ctgcaattaa tccacaggct gccatgatcc
     3241 ctttattctt caggatttct tctcctgatg catttacgat gatacaaaat acagtatata
     3301 tgatgattcc tgctccgatc ccgatctgct gtcgttttga catcacaaac agacgtggat
     3361 acatttctgc tgtcaacaat gccacaataa agctcagtgc aaatgcccac cggttgtttg
     3421 cataggaaaa cccattaaat gcatatccaa agattgggag aatcaaagca atgatacttg
     3481 ctataaatgc cgctctgtat tttttatgtt tcattcgctg tgaaaacagt gtaaataata
     3541 caacgatccc aacgatcggc agatatcctg cgttcgtatt gctgcccata tttccgtatc
     3601 caataaagtt ttcaataaac agcagataat atttccctgg atatacgatc aatgatttcc
     3661 agtcaacccc attgccgtaa cggccatttc ctagaaatcc tatgacggat ggcagtaaaa
     3721 tcactgctgc cattgcaatt ccaagtgtat aatatattcc aaacttcaag atcgtcagaa
     3781 aaaagttttt catcttgttt tcttcggtgt atttataaaa acggatccac gcatagatca
     3841 ccgcaaatat cgtcagcata tataaaaaat aataattgct cattgctgat acgcagactg
     3901 cgaaaataaa tacatacggt ctttctctct gatagatctt ctcaatccca agacataaca
     3961 gcggaaaata gatcatcgga ttgatgaaga atggatggcg cagtcctagt cgaaagcaaa
     4021 atccgctgaa tacatagacc agtgccccga tcactgttcc ataacttcgt tttttcattt
     4081 ttcttgcata catgataaac gtgatccctg ccagatacat tcgtaacaag atcatgaaat
     4141 catacatagt ttctgtattc tttggcgaaa caaatccata gaggaggttt aacggatcac
     4201 cgatcgcata gtaattcaat gttgagagga tatctgcacc atacccaaga ctcatatccc
     4261 acatcacaag ttttgggtgt cccgccagaa gattacggaa aaattctctt agtgcttccc
     4321 cataatatgc taatgctgaa aaatgctggc ttaatccgtc ttctactcct gctccccata
     4381 caaagctttt tccttcggta aaaaatggca gaaatgccac gatacacatc agtaaaaaca
     4441 tcagtgtata gactccatag caatactttc tttgttcttt tatcattata cttttccttt
     4501 accctttacc cttttttgtt cattatcacg cttagtatac cacattttgg attgaaaatt
     4561 cctgaaaaat gtcttaagat attggatgta tgtacatttt agaatatttt atatctatcg
     4621 gaggtaatat gatatgttaa atttgataaa gaaattatat aatgattcgc gaattcgtta
     4681 tttgtttttt ggtggattga caaccctcgt taatcttgga atgtttgctt tattaaaagg
     4741 accatgtgga attgattata agatcagcaa ctttttctct gttgcgattg cgatctgttt
     4801 tgcatttgtt gtaaataagc tatatgtatt tcagtctaaa tctgaatcct tcacagatac
     4861 aattcatgaa ttcatcaagt ttgttctcgg tcgtcttgta accatggctg tcgaagtggg
     4921 cggagttcct ttctgtgttg agattcttca tcagccacag atgatcgcca agttagaaac
     4981 acaagttatt gttacggttg tcaattattt tatcagtaaa tttcttgttt ttaaatctca
     5041 aaaaaaatga ttccctgtat cacataaaat ccctgagatc agatattcat ctcatccgat
     5101 ctcagggatt tgtattgtta ttctttggat ttgatcactt ctgagataat gtatctcgga
     5161 cgcatcttca cttcatcata aatcttcgca atataatatc cgatcacacc taagctgaac
     5221 atcaaaatgc ttccaaccaa cagtaacaac aagattactg ttgtgaatcc tgcaactgca
     5281 tgtccagaaa aatacatata aagcgattgt attccaagta gtactgcaaa tacgaagaat
     5341 acaacgccaa aaaatgtgac gatctgcatt ggtgcggcgc taaatgatgt gatattattc
     5401 actgcatatc gtactaatga tttgaatgac catttggatt ctcctgcttc tcgttcctgc
     5461 acatcaaatt ctacgtatgt tgtcttgaat cctacccagg atgacaatgc acggaaaaat
     5521 acatttcgtt ctggaagttt tagaaattct tctgctgctt gtcgatccat caacttaaaa
     5581 tcagatgctc tggacatatc gattcctgtc gcatcactga taatactgta aaatgttttt
     5641 gcaaacatct tgtgaatgaa actttctctt cctctggatg ctttcactcc ctcgatcacc
     5701 tgatatccgt cttcccataa acgatacatc tttataagtg tttctggcgg atgttgaaga
     5761 tcacagtcca ttactgcaat acagtctcct tctgcctgtg tgatccctgc aaaaacggct
     5821 ccttcttttc caaaatttct ggaaaagcag actcctttga tatgtgctct ttcttccttg
     5881 agttctaaga tcttctccca tgtatgatct ttagatccgt catttacgaa tactaattca
     5941 tatgggatct gttctttctc aagcaggtct cctaccacct gtgctgtttt cttgatcatc
     6001 tgttcttcgt tataagctgg aagaacaacc gataacatct tatccattgt aaatatctct
     6061 ccttatattt tattctaccg taatctttgc actgacgttc ttctcaatcg acatattctt
     6121 gatctttgtc agctgaacct gtaccgtatg cgttccgggt tcatacagct gcatgtcaat
     6181 ctttggattt aaatccttca ctacaacttt atctactact gagcgtaatc cgctgatctt
     6241 cacctttaac tctgagtcat caaatttgac atcataattc ttcgtattat tgatcaatgc
     6301 gatctgttct gtcggaatcg tcaatgttcg tttcgtcttc ttctcgatgt tcgcacggat
     6361 cacgatattc ttgatcttat ctacagattt tacaaatgta ataccatctg gcagtttaat
     6421 atcggacgct ttgatcgttt tttcaatact ctttgtactg tcactgatat ctaaattcgc
     6481 cagtttgata ctgctgatct tctccagatc atcttcagct ccagtaattt ctatatgttt
     6541 tggttcataa tcaatgcttc caagtacata tcccttgccc ggcgttcctt ttgtttccac
     6601 tgtaaatggt attgtcttcg ttcttgataa tccaatcttt actttaatct tggtcgtatc
     6661 aagtttgatg cggtcctgac ttacttcatc tccgtcttca tcataacact tgacattctg
     6721 agccattgtt acatctttct tcaatccgga tacatctact ttcgctacga tctgtttgat
     6781 cctgcttacc acactctttg gcccagatac agagatcaga ttcggcgttg ctgtctgcgt
     6841 gcttaccgta tatccactcg ctggtgttcc tttcaccaca acattgactg gtatctggtc
     6901 actcttcaca tcttccaata cgactttgac cgttgcacca cctgtatcga tatctaactg
     6961 actcttataa cgtaaagctt caacttcgat ctggatcgca tgaacatctg acatagaaga
     7021 tacatctgcc gttgcgcgga aatctgactt cgtcaaacga tctacaacat ctttctttcc
     7081 cttcacagta aagcttacgg tatctccaga ttcaatctta taagtctgat taagatcgtc
     7141 taaaacctgt tcattcagca tatcaaccgg tacatcgctg aaagtcttct tgatgaccgg
     7201 gttatcggtg ttgataacaa acatccaaag tagaaaggca aaaacaacag aaattatttt
     7261 taaaagcaga tcactttcca ttcttttttt catttcgatt ccgccccttc catagtttga
     7321 atttcttatt ctgtcctttt tcttctctca ggacagccag ttcatctctc aatctctgtg
     7381 gatccagatg tctgatgatc tttccatccc ttgcgataga aataccacca gtttcttcgg
     7441 atacgatcac agtgatactg tctgaaactt cactgatccc tactgctgct ctgtgtctgg
     7501 tacccatttc ctttccaatc tctacactat cgcttaatgg aagatagcaa gttgctgaca
     7561 cgatcctgtt gttgtgaatg atcaccgcgc catcatggag tggtgtattg tgttcaaaga
     7621 tattgatcag aagctggctg ctgaccttcg catcaattgg aattcctgta cgctcatact
     7681 ctccaagtgc tacttctctt tgcagtacga tcagtgctcc tgtctttacc tttcccatct
     7741 caatcgctgc tcttgatatc tcttgcagag ttcttgctgt atattccagt tcttcctgac
     7801 ggctatctgt agagaaaata tcagtcaccc agttctttct tccaagctgt tccaatgcgc
     7861 ttcggagttc tggctggaag acgatcaaaa tcgctgtgat tnnnnnnnnn ncccacaccc
     7921 attgtattgg aaagaatcca tgaaactgta tgtaattgca gtaaatatgc cgcaaatgca
     7981 atacaaagca agataatgat cccttttaat aatacccagg ctcttgttgt tttgatccat
     8041 ttgacgatca gatagatcat aacactgacg atcagaatat ctacaatgtc attcagaccg
     8101 atcttcggaa tcgatgtcca atatatatat ttttcaagtc catcaaaaaa tttactcaaa
     8161 tttcctctcc cttttctttg tcctgttctt tatgttcttg tttatgttct tttcttttta
     8221 aactaaatct atgtgatggt accgtgatct gtgtaacatt aaattcttct tcttccacag
     8281 tcactttcgg tatcgctctt acataataat aatattcttc atcttcaaat tcaaatgttt
     8341 cttctctggt atagtcgatc acattgtata aaaactgtat gatcagggcg atcgcaatgc
     8401 ttcctatgat cgttccgatc tgtgatgtga tattgatctc attgttaacc agatatcctc
     8461 ctatgaacat gcagatcaga tatacaactc ctcctgcaag gattgaaata ttccatccat
     8521 gcgctgctcc ccgcttgcga attgctgtta cgagtgctgt caccagacag aataccacaa
     8581 aatacaaaag cagatcctta ttgtcgatcg ctcttgctgc gatcagactg tagaccttcc
     8641 catgtgctga tcctgatgcc agctggttca ttgtctgctg aaggatattg gacagataat
     8701 agatcactgt tcccattaag atcgctggaa gtcctgcaat tccacaaaac atcccaacaa
     8761 agatcggtaa acatgcaggc agttgaaaat aaaagaagat tggcaccatc atcagataaa
     8821 tccttgtctc tggaaaaact ctctgatata caagaaaata taatacaact accagtgcaa
     8881 aaaataatgt cacttctaac gatacatgaa gtaactctaa gaatataacg acataaaaga
     8941 tcagcgataa tgctgatatt ggaataaaaa cactgatcac tcccatcata aagataaaaa
     9001 atggctgtga aaaggtctct gtatatccaa acaattcccc taatacataa aataaaacag
     9061 ttcctccaag gaatctcatc cctgattgaa tatatcttcc tctcgccgtg aggaatttca
     9121 aaaatcgttc tctatatatc aataattttt ccatcttcgt cttcctcctc gactatcgtt
     9181 ggactaatgt cttcattctc tttttcttta tataatgttt ctagttcttc taaatgcttg
     9241 tcatagatca gtacccttgc ccttacctca tcatactgcc tggatgctcg tatcttcgta
     9301 aaatgcaggt agacaaaaaa taccgcaaca taaattccta tgatcacagc cgcagatact
     9361 ataactttac cttctctcag cttcatgatc acaatatcta actgcatgat acacaacaat
     9421 cctgtcatga tcagaaatga gatcaacaag gcaatgcttg tcttgatctt ctccgatctg
     9481 acataatctt tcttggtata tttgtaaata cggaaatcac cttttccata attcttctca
     9541 tacttggcca tctcagccat aatctttact ttctcttctg aaatcatatg ctttcacctg
     9601 ctttttcagt cttattttta ttatagcatg gaatatagca aaaaaaaagg aggtatgaaa
     9661 actcatacct cctaaatcaa ttcttcttat ccgaagtatc tctttaataa tccttcaaat
     9721 gctttaccat gacgtgcttc gtctcttgcc atctcatgaa ctgtatcatg gattgcatct
     9781 aagtttgctg ctttcgcacg ttttgcaagg tcaaatttac cttctgttgc tccatgttct
     9841 gcatctacac gcatctctaa gttcttcttt gtgctgtctg ttacaacttc acctaataac
     9901 tcagcaaatt ttgctgcatg ttctgcttct tcgtatgctg ctttctccca gtataatccg
     9961 atctctggat atccttctct gtgagctact cttgccattg caaggtacat tcctacttca
    10021 gaacattctc cttcgaagtt tgctcttaga tcagctaaga tatcttcgct gacacctttg
    10081 gcaacaccta ctacgtgttc tgctgcccat tctttttctc cttctacctg ctctttgaat
    10141 ttatctgctg gtactccaca ctgtggacat ttctctggtg gctgttctcc ttcgtgtaca
    10201 tatccgcata ctgtacaaat ccatttcttc ataattcttg ttctccttta tactttatat
    10261 gatcatttat gttgctttgc ctttcggctt agtgtctttt gtttaaaaat agtaatgatt
    10321 actattttta acttgtcttt atattaccat cacattttac ttttgtcaat gattttttaa
    10381 acaatttttg cagattcctt taaaataaac aatattctgt gtaatctttc caggaaagtt
    10441 ctcacttgcg atctcattta acttctcatc gatcggtgcc atctccaaat cgatgactgc
    10501 tccacattcc tgacacacaa aatgatagtg tggatcggtg catgcatcgt aatggtctgc
    10561 tcctccttcg taagatagct ttagtatttc ccctcgctca cttaataatg caagatttcg
    10621 atagactgtt ccaagactga tcttaggaaa ctcttctttg atgttcatat ataccatatc
    10681 ggcagttgga tggtctttgc gagtcattaa aaactttatg atggcatctc tttgcttgct
    10741 ttttttcgtt gctggctgca taacctcttc ctttcctctt taattcaaat aataactgtt
    10801 actgttttta gtatatatat ttttatacat tatgtcaacc atttcgaaga aaattgtata
    10861 aatttatata cacaaagcag acaggtattt gatttcctgt ctgctttgtg catttttaaa
    10921 acttagggag tataaccaaa gtgtcagttt tagctctgga atactttatg tggattcaag
    10981 atattctttg gatcaaatgc tttcttaatt ccactcatta actcaactgt ttctggatca
    11041 agagattctc tcaaatatgg cttcttcgca aatccgattc catgttctcc ggatactttt
    11101 ccacctaact gtgcagcttt ctcgtagatc ttctccattg cttctgccat tcgtttttca
    11161 aattcttcct gacttaagtc atcttttaag atataagaat gaaggtttcc atctccagca
    11221 tgtccgaaac tcttgatacg tatatctact tcattatgta agttatgaag gtattctacc
    11281 atttcattga ctttatttct tggtacgacc atgtcatcca tatcagttgt agatcctttg
    11341 atcgcttcaa ggaaggctcc tctggctttc cagatagatt cttctctttc ttctgtatct
    11401 gagatcagta cgtcaagtgc tcctgcctct aaacaaagtt ttgcaacttt atcataatct
    11461 gcttcaatct cttctgttga attgccgtca aatttgagca gaaggtacgc atcggattgg
    11521 gaatctggga attttttacc taggtactct tctgcatcaa tgatgacctc cctctgcata
    11581 aactcaatgg cagttgggat tgctttggac ttgataatga gaggtactgt gtcaattgct
    11641 tcttttaagg ttttaaatgg tatcagtaaa ctaattgcct ttttaggcag agggagtaat
    11701 tttaatgtgg cttttgtgat aaagcctaag gttccttcgg aaccgatcat caggtctttt
    11761 aaggaatatc cggaactgtt ttttacaacc tttcctccaa attccacgat ctttccattt
    11821 ggaaggacaa cttcaagacc tctgacataa tctctggtca cgccatattt tacagcacgc
    11881 ataccaccgg catttgtact gatatttcca ccgatcgttg cggatttctc tcctggatct
    11941 ggtgggtaga agaaatcatg atcttccacg taggcagcaa gttccattaa taaaactcct
    12001 ggttctacgg tgatcgttaa gttttcttca tcaagttcta atatatgatt catgaggctt
    12061 gaatcgatca tgatcccgtg ttcgatcgct acggaagatc ctaccagacc ggttccagct
    12121 cctcttggtg tgaccggaat attattttca tatgcatatt tcatgatctc agagatttct
    12181 tctgcagaag tcgcttttac tacaacatct gggtagcttt gtgctcctcc taattcatca
    12241 tgactgtatt cttcgttgat attttctgca tataaaattc tttcatcgtc ttttatgata
    12301 tttttgataa cttctaaatc agcttgatca aatttcttat aactcatatc tcttacgccc
    12361 ctttcgcagc tttaatcttc tcgatcagtt ttggtacgat atcgtagaca tctcctacca
    12421 gacaataatg tgctgtctta aagatcggtg ctttttcatc ggaattaatg gatacgatgc
    12481 agtcagagtg atccattcca gctacaaact gaatggctcc tgatacacca caggtaatga
    12541 tcagtttcgg gcgtacggtt cttccactta atcctacctg acattttgct tctacccacc
    12601 ctgcttctgc aagtggtctt gtgcaggcta cctgtccgtc taacagttct gctaattctc
    12661 tgatcattgc aaggtcttct tctttcttga ttcctcgtcc tgcaacgact aaaacatcgg
    12721 cagattcgat aaacttctct ggctctttct ttacaatatc taatacatcc acatgtgctt
    12781 tgatcttttc tttatcgatc gcacatgaaa tgatctctcc atgtgtttct tctgttcttt
    12841 ctggtgcatt catgactttg tatcttaccg ttgccatctg tggacggtta tttggtgtta
    12901 agatatgtgc catgatgttt ccaccaaatg ctggtctaat ctgagaaagg tctgtgttct
    12961 catccatctc aagaatcgta cagtctgctg ttaatcctgt cttcattctt gcagctaccc
    13021 ttggtgccaa ctgtcttccg actgttgttg ctcctgtcag gatcgcacat ggctgatgtt
    13081 tcttgatgaa atcttcaaat acagatgtat aagattctat tttgaatctt gctaactctg
    13141 ggaagtcgta aacaaatact ttatcaactc cgtagtgtaa taattcttct gccttatctg
    13201 aaatgttatt tcccataaat aaagcataga ctggatgatt gatcnnnnnn nnnnttactt
    13261 gccagctctc gtgcttttcc gatcaattcg tatgttactg gatgaatttc tccatcaaca
    13321 tgatcgacat aaacagcgat tccgttccac tgactcttat caatttcttc ttttttctca
    13381 tcttctacat attctactgc tccagctggt cctttcttca cacataattt acacatctta
    13441 catgcggcat tgatagaaag ttttccgtct ttttcttcca tcgcaccgaa tggacaaata
    13501 tcaaccagaa cctgtggatc ttctactttt tcctgatgta ttactaattt tggcattctc
    13561 atttccccct tttagataaa tttcatatct gtgatcatat ggaataattt gtctgcgact
    13621 tcacttccgt ctccgtccca tacttcatga tggtcattcg ctgttggagg gaaaattcgt
    13681 tttacctgtg ttggtgaacc atttaatcca taatgtgttt cgtctttgtc ttccatatca
    13741 tctaaggaat atacttcaat ttcacggtct gcggtatcca tcttctttac ataggatgga
    13801 agtcttggct ggaaaatgtc tttatctacg gataacaaac atggatactg aactttagca
    13861 atctccagtt cttccggcag atccatctcg atcgtgatcg catcggattc tactttagat
    13921 atcttcttta catttgatac gcttgggata tttaaccatt cagatacttc ggaaccaacc
    13981 tgtgctgtgt caccatcagt tgtctgtttt ccaca
//
LOCUS       DAOMUE010000023        13834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_975_length_13834_cov_2.616590, whole genome shotgun sequence.
ACCESSION   DAOMUE010000023 DAOMUE010000000
VERSION     DAOMUE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 13834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13834
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_975_length_13834_cov_2.616590"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..1300
                     /locus_tag="PLC71_02270"
     CDS             299..1300
                     /locus_tag="PLC71_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HRM01506.1"
                     /translation="MEKFINQSPLFLVIICLIIGFAFLMKGADYFVEGSSSVAKRLKV
                     PSIIIGLTIVAMGTSLPETAVSVTASIANNNALAISNVVGSNIFNMMFVIGVCSILAT
                     IGVKRETIIRDIPLSLVCATLLLIFGYLAFGDRSHMILGRFDGIIFLIIFAVYVLMMV
                     FQAMRARNNQEVVQLETMENEELMIMSYPKSLLCIVGGAAAIAFGGDLTVDAASRIAI
                     DFGMSQNLVGLTIVSIGTSLPELVTSIVAARKNEVDMAVGNAIGSNIFNILMVLGIAS
                     AISPLQLIGENVIDIIVLVVFSVVVWIFGATKKKISRTEGIAMICMYLCYSVYIIMR"
     gene            1365..2354
                     /locus_tag="PLC71_02275"
     CDS             1365..2354
                     /locus_tag="PLC71_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM01507.1"
                     /translation="MIRLATIGTNFITDWLMEGILELDEIQLTAVYSRNLEKGKQFAA
                     KYNVEKVYDNYEEMAKDPEIDAVYVASPTYMHYAHSITMINYGKHVLCEKPVCSNREE
                     LDILIKAAKEQGVVFMEAMKNVHSPGFHAMMDNLHKIGTVRRATIQYCQYSSRYDKFK
                     KGIIENAFNPKLSNGALMDIGSYCIHFLASLFGKPEKILADSLFLENGVDGAGSVIAS
                     YGDKQAEIIYSKITDSKLPTQIQGENGCMIIEQCPIIKKITIYYRNGETEELQFDKRD
                     NPMIYETKDFIRLIKEHKVDHQFLRSSEIEMDITDEVRRQTGIVFPADKIREQ"
     gene            2396..3166
                     /locus_tag="PLC71_02280"
     CDS             2396..3166
                     /locus_tag="PLC71_02280"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM01508.1"
                     /translation="MRKIIAAACLMAAMTLCVGCSSAKDGSKDTTKATTETKMKVQSK
                     YKVPKITAAKKTDQLADAQKGETIVTMKVKGYGEMQFKFFMKKAPLAVKNFVTLASNG
                     YFDGQIFHRVINDFMIQSGDPTGTGTGGESIWGEDFDNEVCEELLPLRGSLCMANSGA
                     DTNGSQFFIVQAKADTAKKGLEEGNVELTKKQQALFLDQGGYPSLTGSYTVFGQMING
                     YDVLDKISGCKTQDNGSGEVSQPVKKVVIEKMTVSNAE"
     gene            3190..4581
                     /locus_tag="PLC71_02285"
     CDS             3190..4581
                     /locus_tag="PLC71_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRM01509.1"
                     /translation="MLIVALDTSKKEPLYEQIYNSIKEEIITGVLPFGARLPSARRLS
                     KHLDVSRNTVDTAYAQLCAEGYIESKPKRGFFVCQVEELAELHIPVKIEEEEEEIRPE
                     KIPYDFSSAGVDMEQFPYHIWRKLLKEIMINDNSELFQKGNFQGDLELRKAIMYYLRQ
                     SRGVHVHASQIVVAAGMENLLFLLRQILGEKVSIGIENPVYKNAYEILKELDFKIHPI
                     SMDESGMCVEQLQKTDANLAYVTPAHQYPTGVIMPIGRRSQLLGWAKKDPDHYIIEDD
                     YDSEFRYHGKPIPAMQGLDDGENVIYMGTFSKAIAPAIRASYMVLPKKLAKRYQQIGQ
                     TFSCAVSRIDQKVLTLFLTEGHFERHLNRMRNIYKEKHDLLIKEIKKLNSDIQIYGHG
                     AGAHIIVRFPVSDQKEFQKKLRQKGVMIYPLSWYYIEGKPLKEEYILGFTRMKKEDMI
                     KGIEIIGEVLYSK"
     gene            4600..5985
                     /gene="clpX"
                     /locus_tag="PLC71_02290"
     CDS             4600..5985
                     /gene="clpX"
                     /locus_tag="PLC71_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRM01510.1"
                     /translation="MSEEKNQNEIEEVTNEDEHKDVEHYCYLCRRPESAAGKMLELPN
                     NIFICTDCMQRTLNSMGNGQFGFIDARNMDLDSMDITKMMNDMQSMQPKAEVKKKKKD
                     KKEEPEFTLKDIPAPHQIKAMLDEYVIGQDYAKKVMSVAVYNHYKRVVTNTMDEIEID
                     KSNMLMIGPTGSGKTYLVKTLARLLKVPLAITDATSLTEAGYIGDDVESVLSKLLQAA
                     DNDVDRAEHGIVFIDEIDKIAKKQNTNSRDVSGESVQQGLLKLLEGAEVEVPVGATSK
                     NAMVPLTTINTRNILFICGGAFPNLEGIIKKRLMKKTSIGFGADLKDCFDEEENIIAQ
                     VTNEDLREYGFIPEFIGRLPMIFTLEGLTKEMLVKILKEPKNAILKQYQKLLELDEVK
                     LEFDEGALEAIAEQALKKKTGARALRAIIEKFMLDIMYEIPKDDTIGSVTITRDYIEN
                     HGNPEIHLRDQ"
     gene            complement(6091..7116)
                     /locus_tag="PLC71_02295"
     CDS             complement(6091..7116)
                     /locus_tag="PLC71_02295"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRM01511.1"
                     /translation="MEKKNIDWANIGFGYMPTDYRFVANYKDGAWDEGELTTDANITI
                     SECAGVLQYAQTCFEGMKAYTTEDGHIVVFRPDLNASRMADSCRRLEMPVFPEDKFVE
                     AIEKVVAANEAFVPPFGSGATLYIRPYMFGSDPVIGVKPANEYQFRIFTTPVGPYFKE
                     GAKPITIRVSDYDRAAPRGTGNIKAGLNYAMSLYPIMEAHRQGFDENMYLDPATRTKV
                     EETGGANFIFITKDGKVVTPKSDSILPSITRRSLVYVAKEYLGLEVEEREVLFDEVKD
                     FAECGLCGTAAVISPVGKIYDHGNEICFPSGMEKMGPVIQKLYDTLTGIQMGHIEAPE
                     GWIKVIK"
     gene            complement(7235..8293)
                     /locus_tag="PLC71_02300"
     CDS             complement(7235..8293)
                     /locus_tag="PLC71_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01512.1"
                     /translation="MLKKNKSIALVMILALLIITSSCAPKNTQAEIDKPAKTSASWER
                     KLCRSKLDIISSYGDDQAFHPKVLNFEKPWNGYRYWMAYTPYPGADQRKENPHVCVSN
                     DRIHWKPFEGEGKAVSLDPLTPFEDPDKQYNSDTHLVYRKDIDTLECYWRQVDNRTRI
                     DKIMKRTTTDGIHWSKAQNVLVSDARTDRILSPAIIYENGRYKMWTVNCKRKFPVLYR
                     ESKDGFHWTTPSTIQLKYPSDRLRSWHLDVIHTEKGYEMLVVAFYKGHRHREMNLYYT
                     SSKNEHDFKKCITILKPSEKEKAWDNRGIYRSSFFVENGIYYIYYSGIGYPKGPNSSQ
                     GVGLTYGPSVKNIHGYDA"
     gene            8520..10475
                     /locus_tag="PLC71_02305"
     CDS             8520..10475
                     /locus_tag="PLC71_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HRM01513.1"
                     /translation="MDQMKYLKELAVRYPNVAAASTEIINLQAILHLPKGTEHYVSDV
                     HGEYEKFSHIIRNGSGAIRSRIEDEFGNTLTTKQKKNLASVIYYPEQKMDLMEEELPK
                     NFLLEWQHDTMVRLVHVARSVGNKYTRSKVRKAMSPEFAYVMEELMVEHRRADKKRYV
                     EQILETIITTGRVRQFIAAMAHLIQDLAIDHLHVIGDIYDRGSGPHRIMDCIMKTANV
                     DIQWGNHDILWMGAASGHRACICNVVRICARYNNLDVLENGYGINLIPLARFALECYK
                     DDECELFHASGEVDESNIREEELNKKMHKAIAIMQFKVEGQLIKRRPDFLMDQRLLLD
                     KIDYEKGTITLDGKEYELKDKNFPTIDPNDPYKLTKEEEYVMEHLVTVFKYCAYLQEH
                     IRFFFAKGHLYKVFNGMLLYHGCVPLNEDGTFREVEIEGRKYAGKELYDVLEHLARQG
                     YYEEKDMKSRKYGQDIMWFIWSNENSPVYGKAKMATFERYFLDDADLKKEKKDYYYQW
                     YENEAVINQILEEFGLDTSTGKIVNGHMPVAVKKGESPIKCGGKLFVIDGGFSKAYQK
                     TTGIAGYTLVSNSYGLKLVAHEPFVSRTIAIREETDIHSDTIVINKVTKRKCVMDTDN
                     GKALQEKVADLEQLLWAYQKGLITERI"
     gene            10560..12236
                     /locus_tag="PLC71_02310"
     CDS             10560..12236
                     /locus_tag="PLC71_02310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRM01514.1"
                     /translation="MLCENAAQSNDYFEFIGEYSGVLDYVKEEFNTECITVIDQRFAI
                     AYVKKNGRTSIYGQNYPYNTIPRCFGLMDTQMLEDVGVAQVRRSTLDLYGNGVLVGMI
                     DTGIDYEHPAFRYEDGSSKIYSLWDQTIEGDSEDTFLGYGTEYTKEQIEEALKSDVPQ
                     QKVPSKDESGHGTFLAGLIAGNEDNETGFSGIAPNAGLIVVKLRKAKDYLKEYYCIDP
                     KYEAYAETDIMLAVHYIDHIAEQLQRPIVIFLGIGTNLASHLGTGPLDQYLSGRAMLR
                     GVAVVTSAGNEGQARHHYSGQVSQNDAKVEVKVGESEYGFTMELWGLAPNRYYVDIES
                     PSGQKTGRIQGGLSGQRYVTFLLEKTRLIVEYFTVDTSAGAPVIVMRFQNPAPGIWNI
                     YVSDDGVGNREFDLWLPITNFISEDTFFLESTPYNTLVAPSNTGLLISCGSYNSNTGS
                     LAIDSSRGFPRNAVKPDFTAPGVEILGPLPRKRYGRKSGTSVSGAITAGIAALMLEWG
                     IMQKNDMQINTTRIKNYLIRGARRDPERIYPNREWGYGQVDLYETFLGIR"
     gene            complement(12252..13568)
                     /locus_tag="PLC71_02315"
     CDS             complement(12252..13568)
                     /locus_tag="PLC71_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRM01515.1"
                     /translation="MKKASPFICGTVILTLSNFFSRLIGFYNRIFLAGVIGAHQIGIY
                     QLIFPVYLLGFAVCFQGFQIALSKITAEKKATGNINAARYVLRITIILTLCLCIIFSF
                     FVFWYAELICSVFLHEPSCVPCLRLAVLVLPFVGIKNCIHGYCLGIENSGVPAFSLCL
                     EQISRVSSIFLLSVFMIEKMPVPAFLAVCGMAVGEIVSFFFTLIFYFLHKKSADSLSK
                     STLSASKLSVFHELLSLGIPLTLNSFSVTLLQSVQSILIPMMLYRFYGNRYRSVEIYG
                     ILSGMALPFIMFPSTITNALSLMLLPKISAAKTHNDPSYLRRCAILPLIFCTFLGIGA
                     FGGFFVFGPWIGNFFFHDPLCGKYLRSLSFLCPFLYCSSILSTILNGLGKTKDTLLHN
                     SVSLVIQICFILFAIPLYGVNGYLWGLFLSCLFLCFVNYRKVMLHV"
     gene            13704..>13834
                     /locus_tag="PLC71_02320"
     CDS             13704..>13834
                     /locus_tag="PLC71_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRM01516.1"
                     /translation="MKLSTKGRYGLRAMVDIAVYSQDSPVPISAIAERQNISVRYLE"
BASE COUNT         4832 a         2294 c         2991 g         3717 t
ORIGIN      
        1 caacataatt aattttatta tacggtttta tgattcaatt tgcaatttaa actatacttg
       61 acagatcaat atttttttgt taaagtaatc aaacaatcaa tattccatga gggagtagtt
      121 cgcgttatac aaagacgtga tttgtcaaca gactcggtaa tgactcgcag atatcaagca
      181 agtcagcctg gcaaatatta aagaatgaga ctcatgtatg ttcttatagt acatctggaa
      241 catacatgag tttttcttat gtacaaataa aaagacaatt ttgaaaggag aaaaacaaat
      301 ggagaaattt atcaaccaat ccccattatt tttagtgatc atctgtctga tcataggatt
      361 tgcattttta atgaaaggag cagactattt tgtagaagga agttcaagtg tcgccaaacg
      421 tttaaaagtt ccttctatta tcataggatt gacgatcgta gccatgggaa catcacttcc
      481 agaaactgca gtcagtgtaa cagcgtctat tgccaataat aatgctctgg cgatcagtaa
      541 tgtagttggt tctaatatct ttaatatgat gtttgtgatc ggagtctgtt caattttagc
      601 aacgatcggt gtaaaaagag aaacaattat aagagatatt ccattatctc ttgtatgtgc
      661 gacattactt ctcatatttg gttatcttgc attcggagat agatcacata tgatccttgg
      721 acgttttgat ggaatcattt tccttattat atttgccgta tatgttctta tgatggtttt
      781 tcaggcaatg cgtgccagaa ataatcagga agtcgtacaa cttgaaacaa tggaaaatga
      841 agaattgatg atcatgtctt atccaaagag tctgttatgt atcgttggag gtgcagcagc
      901 cattgcattt ggtggagatc ttacagttga tgccgcaagc aggattgcca tagattttgg
      961 aatgagccag aatcttgtag gattaacgat tgtttcgatc ggaacgtctc ttccagaact
     1021 ggtcacatcc atcgtagccg caagaaaaaa tgaagttgat atggcggtcg ggaatgcgat
     1081 tggttccaat atctttaaca tccttatggt acttggaatc gcatctgcga tcagtccatt
     1141 acagctaatt ggtgaaaatg tgatcgacat catagtttta gtcgtatttt cagttgtagt
     1201 atggatcttt ggagcaacga aaaaaaagat ttcaagaacc gaaggaattg caatgatctg
     1261 catgtatctt tgctatagtg tgtatattat tatgcggtag gcgtgatcat tgacattaca
     1321 ctgtcacaat tataaaatat aagatatatg atggaggaga atcaatgatc agattagcaa
     1381 cgataggaac caattttatt acagactggc tgatggaagg aattttagaa ttagatgaaa
     1441 tccaattaac agctgtatat tcaagaaatt tagaaaaagg gaagcagttt gcagcaaaat
     1501 ataatgtaga aaaagtatat gataactacg aagagatggc aaaagatcca gagattgatg
     1561 cggtttatgt agcgagtcca acttatatgc attatgcaca ttcgatcaca atgatcaatt
     1621 acggaaagca tgtgctttgt gagaaacctg tttgctccaa ccgtgaggaa ttagatatcc
     1681 tgatcaaagc agccaaagaa caaggagttg tatttatgga agccatgaag aatgtacatt
     1741 ctccaggatt ccatgcgatg atggacaatc ttcataagat tggaacagtg agaagagcaa
     1801 cgattcagta ttgtcagtat tcttcaagat atgacaaatt taaaaaaggg atcattgaaa
     1861 acgcatttaa tcccaaatta tcgaatggag cactgatgga tattggatct tactgtatcc
     1921 atttccttgc aagtttattt gggaaaccag aaaagattct cgcagacagt ttgttccttg
     1981 aaaatggagt ggatggtgca ggaagtgtga tcgcatcgta tggtgataaa caggcagaga
     2041 tcatatattc caagattaca gattccaaac tgccaacaca gattcagggt gaaaatggat
     2101 gtatgatcat tgaacaatgt ccgatcatca agaagatcac gatctactat agaaatggag
     2161 aaacagaaga attacaattt gacaaaagag ataatccgat gatctatgaa acaaaagatt
     2221 ttatcagact gatcaaagaa cataaagtgg atcatcaatt tttaagatca tcagagattg
     2281 agatggatat cacggatgaa gtgcgaagac agacaggaat cgtatttccg gcggataaga
     2341 tccgagaaca gtgatcgcaa gtgagcaatc tcttaaaata gaaaggaaat caaaaatgag
     2401 aaagatcata gcagcagcat gtctgatggc agccatgaca ctttgtgttg ggtgttcatc
     2461 tgcaaaagac ggcagcaaag atacaacaaa agcaacaaca gaaacaaaga tgaaagtgca
     2521 atcaaaatat aaagtaccaa agattacagc agcaaagaaa acagaccaac ttgcggatgc
     2581 acaaaaggga gaaacgatcg tcactatgaa agtcaaaggt tatggagaaa tgcagtttaa
     2641 gttcttcatg aagaaagcgc ctttggctgt gaagaatttt gtaactcttg catcgaatgg
     2701 atactttgat ggacagatct ttcacagagt catcaatgat tttatgatcc agagtggaga
     2761 tccaacagga acaggaacag gcggagaaag tatttgggga gaagactttg ataatgaagt
     2821 atgtgaggaa cttctgcctc ttcgcggttc tttatgtatg gcaaattccg gagcagatac
     2881 gaatggaagt cagtttttta tcgtgcaggc gaaagcagat acagcgaaga aaggtctgga
     2941 agaagggaat gtagagctca caaagaaaca acaagcgtta ttcttggatc agggtggata
     3001 tccatcctta acaggaagct atactgtgtt tggacaaatg atcaatggat atgatgtgtt
     3061 agataagatt tcaggatgca agacacaaga taatggaagt ggagaagtaa gccagccagt
     3121 gaagaaagtt gtaatagaga aaatgactgt ttcaaatgca gaataaatta gaggaaaaca
     3181 ggtggttata tgctgatcgt tgcacttgat acttcaaaaa aagagccgtt atatgagcag
     3241 atttataatt ctatcaaaga agaaattata acaggagttt tgccctttgg ggccagactc
     3301 ccttctgcaa ggagactttc caaacattta gatgtcagca ggaatactgt ggacactgca
     3361 tatgcacagc tttgtgcaga aggatatatt gaatcaaaac caaaacgtgg tttctttgtc
     3421 tgtcaggtag aagaactggc agagcttcac attccagtta agatcgaaga agaggaagaa
     3481 gaaatacgac cagagaagat accatacgac ttttcatcag caggagttga tatggaacag
     3541 tttccttatc atatctggag aaaactacta aaagagatca tgatcaatga taacagtgag
     3601 ttatttcaaa aggggaattt tcaaggagat ttggaattgc gcaaagcgat catgtattat
     3661 ctgagacaat ccagaggtgt tcatgttcat gccagccaaa tcgtagtggc agcaggaatg
     3721 gagaatctgc tatttttact acgtcagatt ttaggagaaa aagtttccat tggaattgaa
     3781 aatccagttt acaaaaatgc gtatgaaatc ttaaaggaat tagattttaa gattcatccg
     3841 atctccatgg acgaaagtgg aatgtgtgta gaacaattgc agaaaacaga tgcgaactta
     3901 gcatatgtaa ccccggcaca ccaatatccc acaggcgtga tcatgccaat agggcgaaga
     3961 agtcaattat taggatgggc taaaaaagat ccagatcatt atattatcga agatgattat
     4021 gacagcgagt tcagatatca tggaaaacca attccagcga tgcagggatt agatgatgga
     4081 gaaaatgtga tctacatggg aacattttca aaagcgatcg caccagcgat ccgagcaagt
     4141 tatatggtgc tcccaaagaa acttgcaaag agatatcagc agattggcca gacattttcc
     4201 tgtgcagtat caaggattga tcagaaagtt ttgacgctgt ttctgacaga agggcatttt
     4261 gaacgacatt taaaccgtat gaggaatatt tataaagaga aacatgatct attgatcaaa
     4321 gaaattaaga agttaaactc cgatattcaa atatatggtc atggagcagg agcacatatc
     4381 atcgtaagat tccctgtcag tgatcaaaaa gagtttcaaa aaaaattaag acaaaaaggt
     4441 gtgatgatct atccgctctc atggtattat atagaaggaa aaccattgaa agaagaatat
     4501 attttggggt ttacgagaat gaaaaaagaa gatatgataa aagggattga aattatagga
     4561 gaagtactct attcaaaata aaaagtggag gacaatcata tgtcagaaga gaaaaatcaa
     4621 aatgagattg aggaagtaac aaatgaggat gaacacaaag atgtggaaca ttactgctat
     4681 ctttgcagac gtccggaatc tgcagccggt aagatgttag aacttccgaa taatattttt
     4741 atatgtacag attgtatgca gaggacatta aattctatgg gcaatggtca gtttggattt
     4801 attgacgcaa gaaatatgga tcttgacagc atggatatca caaagatgat gaacgatatg
     4861 cagagcatgc agccaaaagc ggaagtaaag aagaaaaaga aagacaaaaa agaagagcca
     4921 gagtttacat taaaagatat tccagcacca catcagatca aggcaatgct ggatgaatat
     4981 gttattggac aggattatgc gaagaaagtc atgtctgttg ctgtatacaa tcattataaa
     5041 cgagtggtta caaatacaat ggatgagatc gaaatcgata aatcgaatat gctgatgatc
     5101 ggaccaacag gaagtggtaa aacatatctg gtcaagacac ttgcaagact gttaaaagta
     5161 ccactagcga tcacagatgc tacatcctta acagaagcag gatatattgg tgatgacgta
     5221 gaaagtgttt tatcaaagct cttacaggcg gcagataacg atgtagaccg tgcagaacat
     5281 ggaatcgtat tcattgacga gattgataag attgccaaga aacaaaatac aaacagcaga
     5341 gatgtcagtg gagaatccgt tcagcaagga ttgctaaaac tcttagaagg agcagaagtc
     5401 gaagtgccag tcggagctac aagcaagaat gcaatggtgc cactcacaac gatcaataca
     5461 aggaatattc tttttatctg tggtggagcc ttcccgaact tagaaggaat catcaaaaaa
     5521 cgtctgatga agaaaacatc gatcggattt ggagcagatt taaaagactg ttttgatgag
     5581 gaagaaaaca tcattgcaca ggttacgaat gaagatttga gagaatatgg attcattcca
     5641 gaatttatcg gaagacttcc aatgatattt acattagaag gactgacaaa agagatgtta
     5701 gtcaagattc taaaagaacc aaagaatgcg atcttaaaac agtatcagaa gttattagaa
     5761 ttagatgaag taaaattaga attcgatgaa ggggctctgg aggctatcgc agaacaagct
     5821 ctgaaaaaga aaacaggagc cagagcatta cgtgcgatca tcgagaaatt catgctcgac
     5881 attatgtacg agataccaaa agacgacacg atcggaagcg taacgatcac aagagattat
     5941 attgagaatc atggaaatcc agagattcat ctaagagatc agtaaataag aatgatacgc
     6001 caatatgtat aaatgaaatg cagccagaaa gttaaatcaa taactgtctg gctgcatttt
     6061 atgttattta atattctatt aacgaaagtc ttatttaata accttgatcc atccttcagg
     6121 ggcttcaata tgacccatct ggattcctgt taaagtatcg tataatttct ggatgacagg
     6181 tcccatcttt tccattccgc ttgggaagca gatttcatta ccgtgatcat aaatctttcc
     6241 tactggagag ataactgcgg ctgttccaca taatccacat tcagcgaaat ctttgacttc
     6301 atcaaataat acttcacgtt cttcgacttc aagtccaagg tattctttgg ctacataaac
     6361 taaagaacga cgagtgatag atggaaggat actgtcagat ttaggtgtta caactttacc
     6421 atcttttgta ataaagatga agttagctcc acctgtttct tctacttttg ttcttgttgc
     6481 tgggtcaaga tacatattct catcaaatcc ctgacgatga gcttccatga ttgggtaaag
     6541 gctcatagca tagtttaatc cggctttgat attacctgtt ccacgaggag cagcacgatc
     6601 ataatcactg acacggatag taataggttt agctccttct ttgaagtatg gtcctactgg
     6661 tgtagtaaag atacggaact gatattcatt tgctggtttt acaccgatca caggatcact
     6721 tccgaacata taaggacgga tatataatgt agcaccagaa ccgaaaggtg gtacgaatgc
     6781 ttcattcgct gcaacgactt tctcgatcgc ttctacaaat ttatcttcag ggaatacagg
     6841 catctcaagt cttctgcatg aatctgccat acggcttgca tttaagtcag gacggaatac
     6901 aacgatatgt ccatcttctg ttgtgtaagc tttcattcct tcaaaacatg tctgagcgta
     6961 ctgtaataca ccggcacatt cagagattgt gatgttggca tctgttgtta attcaccttc
     7021 atcccatgct ccatctttgt aattagctac aaaacggtaa tctgttggca tataaccgaa
     7081 accgatgtta gcccagtcga tatttttctt ttccataata ttaatgcact tccttttatt
     7141 cgtattgtag agtcgttagc cagtctttgg atggtcggct aaaaattctt attgctaaca
     7201 gtttacacct gaatatttat aacgtcaaga gattttatgc atcgtaacca tgaatgtttt
     7261 tcaccgatgg accatatgtg agtccaacac cctgagagct gtttggtcct tttggatatc
     7321 caataccact gtaataaata taataaattc cattttccac aaaaaagctg ctacggtaaa
     7381 taccacggtt gtcccatgct ttttcctttt cagatggttt taagatcgta atacatttct
     7441 taaaatcatg ctcatttttg gatgaagtat aataaagatt catttcccgg tgcctatgcc
     7501 ctttgtagaa ggcaacgacg agcatttcat agcctttctc tgtatgaatg acatccagat
     7561 gccagcttcg aagtctgtca gatggatatt ttaactgaat cgtgcttgga gttgtccaat
     7621 gaaaaccatc tttactttca cggtataaaa cagggaattt tcttttacag ttgacagtcc
     7681 acatcttata tcgtccgttt tcataaatga tcgccggact tagaatacga tcagtgcgtg
     7741 catcggaaac caatacattc tgagctttac tccagtggat accatcggtt gttgtacgtt
     7801 tcatgatctt atcgatcctg gtacgattat cgacttggcg ccagtaacat tctaaggtgt
     7861 cgatatcttt tctatataca agatgggtgt ctgaattata ctgtttgtca gggtcttcaa
     7921 atggggtcaa tggatcaagt gaaacagctt ttccctctcc ttcaaatggc ttccaatgaa
     7981 tcctgtcatt ggagacacag acgtgtggat tttcttttcg ctgatctgca cctggatatg
     8041 gagtgtatgc catccaatag cgatagccgt tccatggttt ttcaaagttt aagacttttg
     8101 ggtgaaatgc ctgatcatca ccgtaagaac tgatgatatc aagttttgat ctgcaaagtt
     8161 tcctctccca agaggcactt gttttggctg gtttatcaat ttctgcctgt gtgttttttg
     8221 gtgcacaaga agatgtgatg atcaataaag ctaagatcat caccagtgca atgcttttgt
     8281 tcttttttaa cataatctcc tccttattgt aagtagcctg tgaagatcaa ggacagtaaa
     8341 tatggaaatt tacatagatt ttacctaggc tgtttacagt ataacataag aaacagcctt
     8401 gttttcataa aagtataata attaaggaaa atgaaagaaa gttattggga aagtatgaaa
     8461 aataatgcta taatattaca gaacaatcaa aaaagcgtaa acgaaagggg agccttctca
     8521 tggaccagat gaagtatcta aaggaactgg cagtgcgtta tccaaatgtc gcagctgctt
     8581 caacagagat catcaatctt caggcaatct tgcatcttcc caaaggaaca gagcattatg
     8641 tttctgatgt tcacggcgaa tatgagaaat tcagccatat cataaggaat ggatcaggag
     8701 cgatccgaag caggattgaa gatgaatttg ggaatacact gacaacaaag caaaagaaaa
     8761 atctggcatc agttatttat tatccggagc agaagatgga tttaatggag gaagaattac
     8821 caaagaattt ccttcttgaa tggcagcatg atacgatggt acgtctggtt catgttgcaa
     8881 gatcagtcgg gaataaatat acaagatcaa aagtaaggaa agcaatgtca ccagaatttg
     8941 cctatgtgat ggaagaattg atggtagaac accggagagc tgataagaaa cgctatgtag
     9001 agcagattct tgagacgatc atcacgacag gaagggtcag acagtttatt gcagcgatgg
     9061 cccatctgat ccaggatctg gcgattgatc atctgcatgt gatcggagat atttacgata
     9121 gaggatctgg tccacacagg atcatggact gcatcatgaa gacagcgaat gttgatattc
     9181 agtggggaaa tcacgatatt ttatggatgg gagctgcatc tgggcaccgc gcatgtattt
     9241 gtaatgttgt aagaatctgt gcaagataca ataatctgga tgttttggaa aatggttatg
     9301 gcatcaatct gatcccgctg gcaagatttg cattggagtg ttacaaagat gatgaatgtg
     9361 aattattcca tgcatctgga gaagtggatg agagtaatat ccgagaagaa gaattgaata
     9421 agaagatgca caaagccatt gcgatcatgc agtttaaagt agaaggtcaa ttgatcaaac
     9481 gccgtccaga ctttttgatg gatcaaagat tactattaga caagatcgac tatgaaaaag
     9541 gaacgatcac attagatgga aaagaatatg aattaaaaga taagaatttt ccaacgatcg
     9601 atccaaatga tccatataaa ttgaccaaag aagaagaata tgtgatggaa catctggtaa
     9661 ctgtgtttaa atactgtgct tatcttcagg aacatataag attcttcttt gcaaaaggac
     9721 acttatacaa ggtattcaat ggaatgcttc tatatcatgg ctgtgtaccg ctaaacgaag
     9781 atggaacatt tcgtgaagta gagattgaag gaagaaagta cgcagggaaa gaactttatg
     9841 atgttttaga acatctggca agacagggtt attatgaaga aaaagatatg aaatccagaa
     9901 aatatggaca agacatcatg tggttcatct ggtcaaatga aaactcacca gtctacggaa
     9961 aagcaaagat ggcaacgttt gagcgctatt tcttggatga tgcagatctt aagaaagaaa
    10021 agaaagatta ttactatcag tggtatgaaa atgaagcagt gatcaaccag atcttagaag
    10081 aatttggatt agatacatcg acaggcaaga ttgtaaacgg gcatatgcca gtagcagtaa
    10141 agaaaggcga aagcccgatc aaatgtggag gaaagctatt tgtcattgat ggtggattct
    10201 cgaaagcata tcagaagaca acagggatcg caggttatac cttagtgtct aattcttacg
    10261 gattaaaact cgtagcacac gaaccatttg tatcaagaac tatagcgatc agagaggaaa
    10321 cagatatcca ttcagatacg atcgttatta ataaggtaac gaaacgtaaa tgtgttatgg
    10381 atactgataa tggcaaggca ctgcaggaga aagtggcaga tcttgaacag ttattatggg
    10441 cttatcagaa gggacttatt acggagcgta tttaaattaa ttttcataaa ttgtatcata
    10501 cacatagatt aacagatgga aattgggtgc ataaactata aaaaacctga ggtatttcta
    10561 tgctatgtga aaatgcagcc cagagtaatg attattttga atttatcggg gaatacagcg
    10621 gagtacttga ctatgtaaaa gaagaattca acacagaatg tattacagtg atcgatcaga
    10681 gattcgcgat cgcatatgtc aaaaagaacg gacggacatc catatatgga caaaattatc
    10741 catataatac gattccacgt tgtttcggtc tgatggacac acagatgtta gaagatgtcg
    10801 gagtagcgca agtcagaagg agtaccttgg atctttatgg aaatggtgtt ttagttggaa
    10861 tgatcgatac aggaatcgac tatgaacatc cagcatttcg ctatgaagat ggcagcagta
    10921 agatatattc tctctgggat cagacaatag aaggtgactc agaagataca tttctgggat
    10981 atgggacaga atatacaaaa gaacaaatag aagaggcact aaaaagcgac gtgccacagc
    11041 agaaagtacc atcgaaggat gaatccggac atggtacttt tttagcaggt ttaattgctg
    11101 gaaatgagga taacgaaaca ggattttcag gaattgctcc gaatgcagga ttgattgtag
    11161 tgaaattaag aaaagcaaaa gactatttga aagaatatta ctgtattgat ccaaaatacg
    11221 aagcatatgc agaaacagac atcatgctgg cagttcacta tatcgatcat attgcagaac
    11281 agttgcaaag accgatcgtc atattcttag gtattggcac caaccttgcg tctcatcttg
    11341 gaacaggacc attagaccag tatctgtcag gcagagcgat gcttcgggga gtcgcagttg
    11401 tcacaagcgc tggaaatgag gggcaggcaa gacatcatta cagtggtcag gtttcacaaa
    11461 atgatgcaaa agtggaagtc aaagttggag agtcggagta tggatttaca atggaactat
    11521 ggggattagc tccgaaccga tattatgtag atattgagtc accgtcagga cagaaaacag
    11581 gtaggataca aggtgggtta agcgggcaaa gatatgtaac atttttatta gaaaaaacaa
    11641 ggctgatcgt agaatacttt acggtagata catctgcagg agctccagtt atcgttatga
    11701 gatttcaaaa tccggcacca ggaatctgga acatctacgt cagtgacgat ggcgtaggaa
    11761 atcgtgaatt tgatctgtgg cttccaatca caaactttat cagcgaagat acatttttct
    11821 tggaatccac accatataat acactggtag cgccatcaaa tacaggattg ctgatatctt
    11881 gtggaagtta taattccaac acaggaagcc tcgcgatcga cagcagcagg ggatttccaa
    11941 gaaatgcggt taaaccagat tttacagcac caggagtaga aattctagga ccattaccaa
    12001 ggaaacgcta tggcagaaaa tcgggaacga gcgtttcagg agcaattacc gcgggtatag
    12061 cagcattgat gttagaatgg gggatcatgc agaaaaatga tatgcagatc aatacgacaa
    12121 gaattaaaaa ttatctgatc cggggagcaa ggagagatcc ggaaagaatc tatccaaaca
    12181 gagaatgggg gtatggtcaa gtggatctgt atgagacatt tttgggaatt cggtaacagc
    12241 tattttctga ctcatacatg cagcattact tttcgataat ttacgaagca aagaaacaaa
    12301 caactaagaa acagtcccca caaatatccg ttcactccat acaaaggaat cgcaaataaa
    12361 ataaaacaaa tctggatcac aagagataca ctattatgaa gcagtgtatc ttttgttttt
    12421 ccaagcccat ttaatatggt agataagatg gaagaacaat acaaaaacgg acataaaaaa
    12481 gacaatgatc ttaaatattt tccgcaaaga ggatcatgaa aaaagaaatt tccaatccag
    12541 ggtccaaaaa caaagaatcc gccaaaagct ccaattccaa gaaatgtaca aaagataaga
    12601 ggtaagatcg cacaacgtcg aagataagag ggatcgttgt gagttttggc agcagaaatc
    12661 ttaggcagaa gcatcaaaga taatgcattg gtgatcgtag aaggaaacat gataaaaggc
    12721 agtgccatgc cgcttaagat gccatagatc tcgacgctgc ggtaacgatt tccataaaaa
    12781 cgatataaca tcattgggat cagaatactt tgcacactct gtaataaggt gacagaaaaa
    12841 ctattcaaag tcagtgggat cccaagggat aacagttcat gaaatacaga aagttttgat
    12901 gcagaaagag tagactttga caagctgtca gcagactttt tgtgtaagaa atagaagatc
    12961 agagtaaaga aaaatgaaac aatctctcca acagccattc cacagacagc tagaaaagca
    13021 ggaactggca ttttctctat catgaataca gaaagtaaaa agatagagga tacccttgaa
    13081 atctgttcca gacacaaaga aaaagcagga acacccgaat tctcaattcc aaggcagtac
    13141 ccatgaatac agtttttgat cccgacaaat ggaagaacaa gaacagcaag tctcaggcat
    13201 ggtacacagc ttggttcatg aagaaaaact gaacagatca gttctgcata ccaaaataca
    13261 aagaaggaga aaatgataca aaggcacagt gtaagaatga tcgtaatgcg aagtacataa
    13321 cgagcagcat taatatttcc cgtagctttt ttctcagcag tgatcttaga aagtgcaatc
    13381 tgaaatccct gaaaacagac agcaaaccca aggagataaa caggaaagat caattgataa
    13441 attccaatct gatgtgcacc gatcacgcct gccagaaata ttctattata gaagccgatg
    13501 agtcttgaaa aaaaattaga gagtgttaag atcacagttc cgcaaataaa cgggcttgcc
    13561 tttttcatag gaaataccag ccatttttaa tcattgtatg aaataaaaaa aaaagatatg
    13621 acaaataatg ttgacataag atacatgctt tgttaagata acaaatgaaa tccgagttat
    13681 atagtcggga ttgaggtgag agaatgaaat tatcgacaaa aggacgctat ggattaagag
    13741 cgatggtaga cattgcggta tacagtcagg acagcccggt accgatcagt gcgatcgcag
    13801 agagacagaa tatttcagtc agatacttag agca
//
LOCUS       DAOMUE010000024        12702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1117_length_12702_cov_3.134815, whole genome shotgun sequence.
ACCESSION   DAOMUE010000024 DAOMUE010000000
VERSION     DAOMUE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12702
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1117_length_12702_cov_3.134815"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1490)
                     /gene="rlmD"
                     /locus_tag="PLC71_02325"
     CDS             complement(111..1490)
                     /gene="rlmD"
                     /locus_tag="PLC71_02325"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005345752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRM01517.1"
                     /translation="MEFKKNQIVELYIDDIGNEGEGIGHIDGYALFLKDAVIGDKVRA
                     KIIKTKKNYGFARVEEVIEASKDRVSPRCSKARQCGGCTLQHLAYEKQLEYKFNKVKN
                     CLERIGGLENIEEKMEPILGMEEPFYYRNKAQFPVGYDKEGNLITGFYAGRTHHIIDC
                     THCMIQHPVNEQILLKVLDYMKKNNVTAYDEKTHKGLVRHIVTRVGFKTGEIMVCLVV
                     NGSKKNLRNLEMLVDSLTEVEGMTSICVNINKEKTNRILGSKIEEVYGKPYIYDYIGN
                     VKYQIGPLSFFQVNPTQTKVLYEKAMEYADLKGEETVWDLYCGIGTISLFLAQKAKKV
                     YGVEIVKEAIDDARLNAKMNGFDNAEFFVGKAEEVLPREYEKNGVYADTIVIDPPRKG
                     CDSKLLETMVKMAPKRIVYVSCDPATLARDLKVLSEQGYEVEKVCAVDQFSHSSHVET
                     VVGLQRKDT"
     gene            complement(1554..2495)
                     /locus_tag="PLC71_02330"
     CDS             complement(1554..2495)
                     /locus_tag="PLC71_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM01518.1"
                     /translation="MRYISDLHEGDQVSEIYLCKTKSPGTSKFGKSYYSLTLQDKTGM
                     IDGKVWELTNAIGHFEAMDYILVRGQVTSYQGSNQLNIQQIRKAQEGEFDMADYMPST
                     KKDIDGMFKELLAMISKTKNQYLKQLAEKIFIEDKNFAREFKVHSAATSVHHGYIGGL
                     LEHSLSVAKICESYANLYPQLNRDLIVMCALWHDMGKVEELSSFPENDYTDEGQLVGH
                     IVMGAIKLDRLIREIPGFPPKLANEVKHCMLAHHGELEFGSPKKPALLEAIALSQADN
                     LDAKMETFAEIMEKQKEDQEWSGFQRLLDTRIRKTSI"
     assembly_gap    2620..2629
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2664..4724)
                     /gene="ppk1"
                     /locus_tag="PLC71_02335"
     CDS             complement(2664..4724)
                     /gene="ppk1"
                     /locus_tag="PLC71_02335"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRM01519.1"
                     /translation="MKTQQKIFMNRELSWLKFNERVLEEAENKEVPLCERLTFASIYQ
                     SNLDEFFMVRVGSLIDQMLLDKNMKENKTKMTPQEQIDAIIPQVQKLNRRKDSVYEEM
                     MDSLKEHNIHLVNFQKISKKESEYLRAYFQAEIAPLISPTIIGKRQPFPFLKNKEIYA
                     VAVLETKNGKEKLGIIPCGNETFDRLINISGKDAYMLSEEMILHYVPRIFKGYHVKAK
                     TLIRITRNADIDADSMYDEDLDYRDFMVELIKKRKKLAPVRMELSREMDGEIIDLLCE
                     YLELERKYVFYTQTPLDLSFVFKIQDILRKETELFFEKRVPQRSPQFNEEMPIMDQIE
                     KEDKLLSYPYESMRPFLKMLQEAAEDKDVVSIKMTLYRVAKQSKIIASLIEAAENGKD
                     VTILVELKARFDEENNIEWSRQLEDAGCRVIYGLDGYKVHSKLCLITRKKKGKVSYIT
                     QIGTGNYNEKTSRLYTDLSLMTANVDIALEAAEVFQALSMGETVEETDHLLVAPHCLQ
                     NKVIHMIDREIEHAKAGEPAYIGLKMNSLTDKKIMEKLIKASKAGVKIDMVIRGICCL
                     IPGVKGETDNIQVRSIVGRYLEHSRIYIFGTKGREKVYIASADFMTRNTLRRVEVAVP
                     IYNTDIKMQLIEMFITMLSDNVKAREEDHNGNYKIPKNQDTPLNSQEFFYKQAYLNAK
                     NVNS"
     gene            4915..7317
                     /locus_tag="PLC71_02340"
     CDS             4915..7317
                     /locus_tag="PLC71_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRM01520.1"
                     /translation="MNQKVLQTLEFDKIINILTGYATTELGKLMCANLKPMTEEADIL
                     NAQDQTQDALTRIYKRGNVSFFGVSDLSPSLARLKMRGTLGTGELLDIARVLESVKNA
                     VSYGVRMEDDLEADSLDELFESLVPLDDLLHEIRRCIISEEEISDDASSTLKHIRRAM
                     KQTNQKIHTQLTTLVSSASNQDKLQDAIVTMRNGRYCIPVKQEYRSSFQGMIHDQSAS
                     GNTLFIEPMSVVTLNNELKELEGKEQSEIEHILSILSEQASYGVDDLAHNQKTLVLLD
                     FIFAKAKYAKDIDASKPIFREDGIINIKQGCHPLLDRKKVVPINVSLGKDFSMLIVTG
                     PNTGGKTVSLKTVGLLSLMGQAGLHIPAFQGSSLGIFREIFADIGDEQSIEQNLSTFS
                     SHMTNIVSIVQQAHRDSLVLLDELCGGTDPIEGAALAISILSDLHGRGIKTMATTHYS
                     ELKMFALSTDDIENASCEFDVETLSPTYRLMIGIPGKSNAFAISRKLGLDEHIIEGAA
                     DQIDESVKDFETILADLEKSKQTIEKEQEEILEYRKEIETLRKSLKSRQDNIKEKRDK
                     MLRDAREEAHNIISEAKEIADSTIREYNKLKKQNKNPDANKKMEHMRSDLRGRMTKLE
                     GQMAYKSKKKNKKRHEANDFHVGDEVYVTSLSLAGTVSTLPNAKGDLYVQMGMMRSLV
                     NIKDLEITKTAKDVKRENQRNESRNRGRTAINKSASIRPEINVMGMTVDEAIAQLDKY
                     IDDACLANLAQITVVHGKGTGALRKGLHNYFKQLKKQKRISGYRDGEYGEGDLGVTVV
                     IL"
     gene            7342..>7772
                     /locus_tag="PLC71_02345"
     CDS             7342..>7772
                     /locus_tag="PLC71_02345"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HRM01521.1"
                     /translation="MTYYDILFSFFIYGFLGWCSEVAFAAFKQHSFVNRGFLNGPICP
                     IYGIGVTVVVASLQPYVGNLILLYITSTILVTFLEWLTGFLLEKMFHHRWWDYSEMPL
                     NIGGYVCPLFSAIWGVACVLIVKLIYPFTDKLVSFFPIWLG"
     assembly_gap    7773..7801
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7802..8225
                     /locus_tag="PLC71_02350"
     CDS             <7802..8225
                     /locus_tag="PLC71_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01522.1"
                     /translation="IVDICITVQGILKFNKRLALMEDIAKELRNISDQIGENIYDSMM
                     DGLEAQDRLKARADVIREKVDEAKQENADELKARMDSLKQRAEELRSKYEKMAKLPTF
                     TSRRILRAFPKMMPREHVREFGQLRRILKKKRDELKKS"
     gene            complement(8356..8871)
                     /locus_tag="PLC71_02355"
     CDS             complement(8356..8871)
                     /locus_tag="PLC71_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM01523.1"
                     /translation="MGFFFKNKKKNKEEETVTPVVETTPEVPAKEGMILVREGIKLGL
                     PTVSMEEAIVAAGELLRDLGYVDDDYIPAMIRRNEEASVYMGLGLAIPHGTEDAKRDV
                     KRSGIIVMQYPDGVEFNGGTAQLVIGIAGVGDEHLEILGQITEAVTEEEILEELKKTT
                     DVDYILNTFTN"
     gene            complement(9014..10465)
                     /locus_tag="PLC71_02360"
     CDS             complement(9014..10465)
                     /locus_tag="PLC71_02360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM01524.1"
                     /translation="MYNKKKKIKHKTLKIFCLLIGTIVTAVFLEAGILYLVNSQNVYK
                     TSKVLLDQTIEIVEKNKQSEVRTVVADIPVYKGIALYVADKETGRIYGATDVSKIGVK
                     LDDMGLRKQRKKIAKEKIASGIIRVDGKKNYYILKKTQKYIVGVTYWIAVDNKSNLIA
                     ILIMVVYLSIAAVGILFMVLRLSKVKKKNKEQSVMISSISEMSNIDKMTGCFNRKAYD
                     EDISEIRMDSQFIYVSMDVNGLKIINDRQGHAAGDELICVAASCMKCRFNKYGKVYRM
                     GGDEFAAILFVKREQFEWIRRQFDGDIKYWSCNRIKELSISYGYVSSSECQWDSMKEI
                     SDVADIRMYEEKAMYYKKNGVDRQGQPASYVALYRLYTQILRINLEKDRYKIINWEET
                     KNKKKQDSIGALSEWFHNFEDIRLIHSDDLVKYLRKTKIEYLKKQFANKKKFVTITYR
                     RKEGDGYKRITLEIIPENENSQNTYEGFLYVKE"
     gene            complement(10489..11448)
                     /locus_tag="PLC71_02365"
     CDS             complement(10489..11448)
                     /locus_tag="PLC71_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HRM01525.1"
                     /translation="MNIKKVAVLGAGAVGSYVIWGLSNNKNITLGVVADGERNERLKN
                     QGLMINGTKYTPQVWTPEEAHNVDLLIVSLKYGSLRGALKDIQTIVGENTTVMSLMNG
                     VDSEEIIAEKIDKSHILYSFIKVASQKKEDGYHFDPETTVGIYFGELQQPYESERVKA
                     VNDIFENTGLHWIITDDIQAEIWGKFKLNVCNNLPQAILGVGVGCYEDSEHMAAIREG
                     LRAEVEAVAKAKGIDLSMINAKSGRGSAVKASARYSTLQDIDSGRHTEIDMFSGAMMR
                     MGKELGIPTPYNEYTYHMIKALEEKNDGVFEYQGENDRVSWSK"
     gene            11639..12673
                     /locus_tag="PLC71_02370"
     CDS             11639..12673
                     /locus_tag="PLC71_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM01526.1"
                     /translation="MENPFTLTFGQKPTEFISRTNQIGKIIHTFDMENPSNKVYMVAG
                     VRGSGKTVSLAEIADHYSSNDQWIVLRLSADTDLIAGAVSELTRVSQFHDLDLGLNLN
                     LGIAELSVNKTNDSLEKEAMLRNILEKLKNKGKKVLFIIDEIINNSYVKVFASNFQIY
                     ITQNYPVYLVMAGLFDNISNLQNEKSLTFLYRAPKIFLEPLSIPAITTSYRSVFDISP
                     SEAVEMAKLTKGYPFAFQILGYLKWETNDDLEKLLPKFDEELIIYAYEKIWSELSELD
                     RKIVYVISTGVYKTGEIREKLSISPQLLNTYRKRLMERGVVNGSVRGELTLALPRFEE
                     YIEMYCEVNL"
BASE COUNT         3502 a         2545 c         2087 g         4529 t
ORIGIN      
        1 gtctaaggca acaactacgg ttactctctg cacaagtatc ttttccttgt gattttcacc
       61 gattttgttg aaactgaaaa actctacaaa gtgcgtaaat tcaaggattt ctaggtatct
      121 ttcctttgta gtcccaccac agtctccaca tgtgaactat gactaaattg gtcgacggcg
      181 cagacttttt cgacttcgta gccttgttct gataatactt ttaaatctct ggctagtgtt
      241 gctggatcac agcttacata tacgattcgt ttgggtgcca ttttcaccat tgtttctaac
      301 agtttactgt cgcatccctt tcttggtggg tcaatgacga tcgtgtctgc atagactcca
      361 tttttctcgt attctcttgg aagtacttct tctgcttttc ctacgaagaa ttctgcatta
      421 tcaaagccat tcatcttagc atttaatctt gcatcgtcga tggcttcttt gacgatttct
      481 actccataaa ctttctttgc tttctgagct aggaataagg aaatagttcc gattccgcaa
      541 tagagatccc atacggtttc ttcgcctttt aaatctgcgt attccatagc tttttcatat
      601 aatacttttg tttgtgttgg attgacctgg aagaaagata gtggtccgat ctggtatttt
      661 acattgccaa tataatcata aatgtatggt ttaccatata cttcttcaat cttgcttccc
      721 aagatcctgt ttgtcttttc tttgttgata ttgacgcaga tacttgtcat tccttcgact
      781 tctgttaagg aatctacgag catttctaag tttctcagat tctttttgct cccattcaca
      841 acaagacaga ccatgatctc acctgttttg aatccgactc ttgttacaat gtggcggacc
      901 aatcctttat gggtcttttc gtcataagct gtaacattgt ttttcttcat gtagtccaag
      961 acttttaata aaatctgttc gttcactgga tgttggatca tacagtgtgt gcaatcaata
     1021 atatgatgcg tccttcctgc ataaaatcct gtgataagat tcccttcttt gtcatatccg
     1081 actggaaact gagctttgtt tcgatagtaa aatggttctt ccatcccaag gatcggttcc
     1141 attttttctt cgatattttc taagcctcca attctttcta aacagttttt cactttatta
     1201 aatttatatt ctaactgctt ttcataagct aaatgctgta atgtacatcc tccgcactgt
     1261 cttgctttgg aacagcgtgg gctgactctg tctttggatg cttctatgac ttcttctact
     1321 cttgcgaagc cataattttt ctttgttttg atgatctttg cacgaacttt gtctccgatg
     1381 actgcatctt ttaaaaagag ggcataacca tctatatggc cgatgccctc tccttcattc
     1441 ccaatgtcat cgatatagag ttctacgatc tgatttttct taaattccat acgcacctct
     1501 gtatcttttt cttggttaaa ctgttgtatt gatcctattt tattataatt cttttagata
     1561 gaggtcttac ggattcttgt atctaataaa cgctggaatc ctgaccattc ctgatcttct
     1621 ttttgtttct ccatgatctc tgcaaatgtt tccatcttcg catctaaatt atctgcctga
     1681 cttagtgcaa ttgcttctaa taatgcaggt ttctttggag aaccaaattc taattctccg
     1741 tgatgtgcta acatacagtg ttttacttca tttgcaagtt ttggtgggaa acctggaatc
     1801 tcacggatca gacggtctaa tttgatcgct cccatcacaa tgtgtccaac taactgacct
     1861 tcatctgtat agtcattctc tggaaagctt gataattctt cgactttccc catatcatgc
     1921 cataatgcac acattacgat caagtctctg tttaactgtg gatacagatt tgcatagctt
     1981 tcgcaaatct tcgctacaga taaactgtgc tctaaaagcc ctccgatata cccatggtgt
     2041 acgcttgttg ctgcggaatg tactttgaat tctcttgcaa agtttttatc ctctataaaa
     2101 atcttctctg ctaactgttt taaatactgg ttctttgtct tgctgatcat tgcaagaagc
     2161 tctttgaaca tcccatcaat atccttcttg gtacttggca tataatctgc catgtcaaac
     2221 tcaccttcct gagctttacg gatctgttgg atgtttaact ggttagaacc ctggtaactt
     2281 gttacctgtc ctctgactaa aatgtagtcc atcgcctcga aatgtccgat cgcattggta
     2341 agttcccata cctttccgtc gatcattcct gttttatctt gtagtgtcag ggaataatat
     2401 gattttccaa atttggatgt tcccggtgat tttgttttgc ataaataaat ctcggaaacc
     2461 tgatctcctt cgtgcaaatc actaatatat ctcattgctt tgtgcctttc taaattcgtt
     2521 cttcgccagc caactctcct ggcgattttt aaatcctcta ttttttacct atgttattat
     2581 gccacagttt agttgtaaaa tctagtgaaa tttacttatn nnnnnnnnna tgaacgtcat
     2641 gatttcaaaa ggcacaaaac tgtttatgaa tttacatttt tcgcattcaa atatgcctgc
     2701 ttatagaaaa attcctgtga atttagtggt gtatcctgat ttttaggaat tttataattt
     2761 ccattgtgat cttcttctct tgcttttaca ttatcactta acattgtaat aaacatctcg
     2821 atcagctgca ttttaatatc tgtattgtag atcggtacag ctacctcgac tcttcttaat
     2881 gtatttcttg tcataaaatc tgctgacgct atataaactt tttctctgcc cttcgttcca
     2941 aaaatataga ttcttgaatg ttctaagtat cttcccacaa tacttcttac ttgaatatta
     3001 tctgtttcac cctttactcc cggaatcaga caacagattc ctcgaatgac catgtcaatc
     3061 ttcacacctg ctttggatgc tttgatcaat ttctccatga tctttttgtc tgttaaagag
     3121 ttcattttta atccaatata agctggttct ccagcttttg catgttcaat ttcccgatcg
     3181 atcatatgga tcactttatt ctgtagacag tgtggagcta ccagcagatg atccgtttct
     3241 tccacagtct ctcccataga taatgcctga aaaacttccg cagcttccaa cgcaatatct
     3301 acatttgctg tcattaaaga aagatctgta taaagtctgc ttgtcttttc attgtagttt
     3361 cctgttccga tctgtgtgat ataagatact tttccttttt tctttcttgt gatcagacat
     3421 aatttagagt gtactttata accatctaat ccatagatca cgcggcatcc tgcatcttct
     3481 aactgtctgg accattcaat attattttcc tcatcaaatc tagcctttaa ttctacaagg
     3541 atcgtgacat cttttccatt ttctgctgcc tcgatcaaag atgcaatgat cttactctgc
     3601 tttgcgacac gatataatgt cattttgatt gaaacaacat ctttatcttc tgccgcttcc
     3661 tgaagcattt ttagaaatgg ccgcatactt tcatatggat aagagagtaa cttatcttct
     3721 ttttcaatct gatccataat tggcatttct tcgttaaact gtggtgatct ttgaggaact
     3781 cttttctcaa aaaacaattc tgtctctttt cttaaaatat cctgaatctt aaatacaaaa
     3841 gacagatcta acggtgtctg tgtatagaat acatattttc tttcaagttc cagatactca
     3901 cataaaagat caatgatctc tccatccatc tctcttgata attccattcg gactggtgct
     3961 aatttctttc ttttcttgat cagttctacc atgaaatcgc gataatcaag atcttcatca
     4021 tacatagaat ctgcatcaat atctgcattt cttgtaattc ggatcaatgt ttttgctttg
     4081 acatggtatc ctttgaagat tcttggcaca tagtgaagaa tcatttcttc tgataacata
     4141 taagcatctt ttccagaaat attgatcaat ctgtcaaacg tttcatttcc acatgggata
     4201 attcctaatt tttcttttcc atttttagtt tctaacacag caactgcgta gatttcttta
     4261 ttctttaaaa aaggaaatgg ctgtctcttc cctatgatcg taggagagat caatggcgcg
     4321 atttctgctt gaaaataagc tcttaaatat tcgctttctt ttttagatat tttttgaaaa
     4381 tttaccagat ggatgttgtg ttctttcaga gaatccatca tctcctcata tacgctgtct
     4441 tttcttctat ttaatttctg tacctgtgga atgatcgcat caatctgttc ctgtggtgtc
     4501 atctttgtct tattttcttt catattctta tcaagtaaca tctgatcgat caaagatcca
     4561 actcttacca taaagaattc atctaagttg ctctggtaaa tggatgcaaa tgtcaatcgt
     4621 tcacatagtg gtacttcttt attctcagcc tcttctaata cgcgttcgtt aaacttaagc
     4681 caggacagtt cccggttcat aaaaatcttc tgctgtgttt tcatcttttc ctccaaatct
     4741 ctatacgatg taatttctat tgtactggtc aaatgtaaaa tcatactgct ttttatgtta
     4801 agttatcgta aattaacgtc tggaattgcc attttcccta tgtttacagt ccaaacaatc
     4861 tgtgttaaac tctttagtga gttgcaataa tttaatgtaa atgaggaatt tttcatgaat
     4921 cagaaagtat tacaaacact tgaatttgat aagattataa atattttaac aggatacgca
     4981 acaaccgaac taggtaagtt gatgtgtgcc aatttaaaac caatgactga agaagctgat
     5041 atcttaaacg ctcaggatca gacacaggat gcattgacac gtatctataa acgtggaaat
     5101 gtgtcttttt ttggagtaag tgatctttcc ccttctcttg cccgtttgaa gatgagaggt
     5161 acacttggaa ctggggaact tcttgatatc gctcgtgttc tggaatctgt aaagaatgcc
     5221 gtttcttacg gagtccgcat ggaagacgat cttgaagcag attctctcga cgagcttttt
     5281 gaaagccttg ttcctttaga tgatcttctg cacgagatcc gccgctgtat tatttctgaa
     5341 gaagagatca gtgatgatgc ctcttctact ttaaaacata tccgtcgtgc aatgaaacag
     5401 acaaaccaga agattcatac acagttaact acgctggtaa gctctgcatc caatcaggat
     5461 aaattacagg atgcgatcgt tacaatgcga aatgggcgtt actgcatacc agtcaagcaa
     5521 gagtacagaa gttcattcca aggtatgatc catgatcagt ctgcttctgg aaatacgctg
     5581 tttattgaac caatgtctgt tgtaacatta aataatgaat taaaagaatt ggaaggaaaa
     5641 gaacagtctg agattgagca tattctttct atattaagtg aacaggcttc ttatggtgtt
     5701 gatgatcttg cacataacca gaagaccctt gtattattag acttcatttt tgcaaaggca
     5761 aaatatgcga aggatattga tgcatccaaa cctattttcc gtgaagatgg aattatcaat
     5821 atcaaacagg gatgtcatcc tttactagac cgcaaaaaag tcgttccgat caacgtatct
     5881 ctcggcaaag atttctccat gctgatcgtg accgggccaa atacaggtgg taaaactgta
     5941 tctttaaaaa ccgttggatt attatctttg atgggacagg caggccttca tattcctgca
     6001 ttccaaggat cttctcttgg aatttttcgt gaaatttttg cagatatcgg cgatgagcag
     6061 agtattgagc aaaacttaag taccttctct tctcatatga caaatattgt atcgatcgtt
     6121 cagcaagctc atcgagattc tttagtatta ctcgatgaat tatgtggtgg tactgatcca
     6181 atcgaaggtg ccgcacttgc gatctctatt ttaagtgatc tgcacggacg tggaattaaa
     6241 acaatggcga caacccatta cagtgaactt aaaatgttcg cactttccac cgatgatatc
     6301 gaaaatgctt cctgcgaatt tgatgtagaa acgctttctc caacataccg tctgatgatc
     6361 ggtattcctg gaaaaagtaa tgcgtttgca atttcccgca aattaggatt ggatgaacat
     6421 atcattgaag gtgctgcaga tcagattgat gaatctgtca aagattttga aacgatctta
     6481 gctgaccttg aaaagagtaa acagacgatt gaaaaagaac aggaagagat ccttgaatac
     6541 cgtaaggaaa tcgaaactct ccgtaagagt ttaaaatctc gtcaggataa tataaaagaa
     6601 aaacgtgata agatgcttcg cgacgcccgt gaagaggctc acaatatcat ctctgaggcg
     6661 aaagagatcg cagattctac gatccgtgaa tataacaaat taaagaaaca aaacaagaat
     6721 ccagatgcaa ataagaagat ggaacatatg cgaagtgatc ttcgcggccg catgacaaaa
     6781 ttagaaggtc aaatggctta caaatccaag aaaaagaaca agaaacgtca cgaggcgaat
     6841 gatttccatg ttggtgatga agtttatgta acaagtcttt cccttgccgg aactgtttcc
     6901 acgctcccaa atgccaaagg tgatctgtat gttcagatgg gtatgatgcg ttctcttgtt
     6961 aacatcaaag accttgagat cacaaaaacg gccaaagatg taaaacgtga aaatcaaaga
     7021 aatgaaagtc gtaaccgagg acgtactgcg atcaacaaat ccgcttccat ccgacctgag
     7081 atcaatgtta tgggtatgac ggttgatgaa gcaatcgcac agcttgataa gtacatcgat
     7141 gatgcgtgcc tggccaactt agcacagatc actgttgtcc atggaaaagg aactggtgct
     7201 ttacgtaaag ggcttcataa ttactttaaa cagttgaaga aacaaaaacg tatctccggt
     7261 taccgtgatg gtgaatatgg tgaaggagat cttggtgtaa ctgttgttat actgtaaaaa
     7321 agatataaag aaggagattt tatgacttat tacgatatct tatttagctt ttttatatat
     7381 ggatttcttg gttggtgttc tgaagtcgct tttgcagctt ttaaacaaca ttcctttgta
     7441 aaccgtggat ttctaaatgg tccgatctgt ccgatttatg gaattggagt gacggttgtc
     7501 gttgcttctc ttcagcctta cgtcggaaac ttgatcttgc tttatattac ctccaccatt
     7561 ctcgttactt ttcttgaatg gctaacgggt tttcttcttg aaaagatgtt tcatcacaga
     7621 tggtgggatt attctgaaat gccattgaat atcggcggct atgtatgtcc tttattttct
     7681 gcgatctggg gcgtcgcttg tgtattgatc gtgaaactta tctatccttt tacggataaa
     7741 ctagtttctt ttttcccgat atggcttgga aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 ntatagttga tatttgcatc acagttcagg gtattttaaa attcaacaaa cgtcttgccc
     7861 ttatggagga tattgcaaaa gaactgcgta atatttctga tcagatcggt gaaaatatct
     7921 acgatagtat gatggatggt ctggaggctc aagaccgatt aaaagctcgt gcagatgtta
     7981 ttcgagagaa agttgatgaa gccaaacagg aaaatgcaga tgaactcaaa gctcgtatgg
     8041 attctttaaa acagcgagct gaggaacttc gtagcaaata cgaaaagatg gcaaaacttc
     8101 ctacatttac atcacgccgt attttacgtg cattccctaa gatgatgcct cgtgaacatg
     8161 taagagaatt tggacagtta cgacgcatcc tgaagaaaaa aagagatgaa ttgaaaaagt
     8221 catgataaat ttttaactgt aaaaatgtca ggtacatcta agtttctcga aaacttagat
     8281 gtacctgaca tttttttctt tttattttct atccaattaa cacagttttt ccacttcctg
     8341 acgttacgaa agaaactaat tcgtgaatgt attaagaata taatcaacgt ccgtcgtctt
     8401 ctttaattct tccagaattt cttcttctgt cactgcttct gtgatctgtc caagaatctc
     8461 taaatgttca tctccaactc cagcaattcc aatgacaagc tgtgctgttc cgccattaaa
     8521 ttcgactccg tctggatact gcatcacaat aattccgctt ctttttacgt ctctttttgc
     8581 atcttctgtt ccgtgtggaa ttgcaagtcc taatcccatg tatacagatg cttcttcatt
     8641 tcttcggatc attgctggaa tataatcatc atctacatat ccaagatctc ttaaaagttc
     8701 ccctgctgcc acgatcgctt cttccatgct taccgttgga agtcccagtt taattccttc
     8761 ccttaccaaa atcatacctt ctttcgctgg tacttctggt gttgtttcta caactggtgt
     8821 tactgtttct tcctctttat tttttttctt attcttgaaa aagaatccca taattgtctc
     8881 ccttctaaat ccaattttta ttccattatt tttttctatc tctattatag caatgaaatt
     8941 ttttaacgtc tatttttagt agtgctaatt ttttcagtaa attttgtgtc attattttgt
     9001 tttctttgat aaatcattcc ttaacatata aaaatccttc atatgtattt tgtgaattct
     9061 cattttctgg aataatttct aacgttatcc tcttgtaacc atctccttct tttcttcgat
     9121 aggtgatcgt tacaaatttc tttttatttg caaattgctt tttcagatat tcaatctttg
     9181 tcttcctaag atatttcacc aaatcatctg aatgaataag ccgtatatct tcaaaattgt
     9241 gaaaccattc tgacagagct cctatggaat cttgtttctt tttattcttg gtttcttccc
     9301 aatttataat cttataacga tctttttcca gattgattcg gagaatctgt gtatacaatc
     9361 tgtaaagagc tacataggag gctggctgtc cctgacggtc tactccattt ttcttgtaat
     9421 acatcgcttt ttcttcatac atacgaatat cagcaacatc tgatatctct ttcatggaat
     9481 cccattggca ttcactgctt gaaacatatc cataagaaat cgaaagctcc ttgatccggt
     9541 tacagctcca gtacttgata tctccatcaa attgccgtct gatccattca aactgttctc
     9601 ttttcacaaa tagaattgct gcaaattcat ctccacccat tctataaact tttccatatt
     9661 tattaaacct gcacttcatg caagatgctg ccacacagat caattcatct cccgctgcat
     9721 gtccttgtct gtcattgata atctttagtc cattcacatc catagaaaca tatataaatt
     9781 gagaatccat gcgtatctct gaaatgtctt cgtcatatgc ttttcgatta aaacatcctg
     9841 tcatcttatc gatattcgac atctctgaaa tagaggaaat catcacagat tgttccttat
     9901 tcttcttttt aactttcgac aaccttagta ccataaatag aataccgact gctgcaatac
     9961 tcaaatatac aaccatgatc aatattgcga tcaaattact cttgttgtct accgcaatcc
    10021 aatatgtaac acccacgata tatttctgtg ttttctttaa gatataataa ttcttttttc
    10081 catcgacacg aatgattccc gatgcgatct tctccttcgc aatcttcttt ctctgcttgc
    10141 gaagccccat atcatctaat ttcactccta tctttgaaac atctgtagcc ccatagattc
    10201 ttccagtttc tttatccgct acatataaag caattccttt atatactgga atatctgcta
    10261 caacagtcct cacttcactt tgtttgtttt tctctacgat ttcaattgtc tgatctaaca
    10321 acactttgga tgttttatat acattctgac tatttactag ataaagaatt cctgcttcca
    10381 aaaatactgc tgtcacgatc gtacctatca gcagacagaa aatcttcaac gtcttatgtt
    10441 ttattttttt ctttttgtta tacatgcatt ttcccttatt ttacattatt atttagacca
    10501 agacactcta tcattctccc cttgatattc gaatactcca tcattctttt cttctaatgc
    10561 tttgatcata tgatatgtat attcattata tggggttgga atccccaatt ctttccccat
    10621 tctcatcatt gcaccagaga acatatcaat ctctgtatgg cgaccagaat caatatcctg
    10681 taatgtcgag tatcttgcag atgctttaac cgcactcccc cgtccagact ttgcattgat
    10741 catacttaag tcgattcctt tcgctttagc aactgcttca acttctgctc ttaatccttc
    10801 tcggatcgct gccatatgtt cactatcttc atagcatcca actccaactc ccaagattgc
    10861 ctgtggcaga ttattgcaga catttaattt gaatttgccc cagatttctg cttgaatatc
    10921 atctgtgata atccagtgaa gacctgtatt ttcaaaaata tcatttactg cttttactcg
    10981 ttcgctttcg tatggttgtt gtagttctcc aaaataaatc cctactgttg tttctggatc
    11041 aaaatgatat ccatcttctt tcttctgtga tgctacttta ataaaagagt ataaaatatg
    11101 tgatttatca attttctccg ctatgatctc ttcactgtca actccattca tcagactcat
    11161 gaccgttgtg ttttctccta cgatcgtctg aatatccttt aatgcccctc tcagacttcc
    11221 gtattttaaa gatacgatca gaagatccac attgtgtgct tcttctggag tccacacttg
    11281 cggtgtgtat tttgttccgt tgatcattaa tccttggttt ttgaggcgtt catttctctc
    11341 accatctgct acaactccca gtgtaatatt tttattattt gacagtcccc agataacgta
    11401 agaacctacg gcacctgctc caagaactgc tacttttttg atgttcattg ttttttggtt
    11461 cctcctgatc gtcgtttttt gattgttatt cttattttaa catttttgga gtgtcttttg
    11521 tgtatattct tgttttattt atatttattt attataataa acataaataa atataaataa
    11581 acatatattc atataataaa cataaataaa cataaataaa tatggaggtg aatagaaaat
    11641 ggaaaatcct tttactttaa catttggtca aaaacctacg gaattcattt cgcgcactaa
    11701 ccaaattgga aagatcattc atacatttga tatggaaaat ccttcaaata aagtttatat
    11761 ggttgctggt gtcagggggt ctggaaagac ggtttctctt gctgaaattg ctgatcatta
    11821 ttcttcaaat gatcaatgga ttgttttaag attgagtgct gatacagatt tgatcgctgg
    11881 tgcagtttcc gaacttaccc gcgtttcaca atttcatgat ctggatcttg gattaaatct
    11941 aaatctcgga attgctgagt tgtcggtaaa caaaacaaat gattctttgg aaaaagaagc
    12001 tatgcttcgt aatattcttg agaagttaaa aaataaagga aagaaagttc tttttatcat
    12061 tgatgagatt ataaataatt cttatgtcaa agtatttgct agtaattttc agatttatat
    12121 tacacagaat taccctgtgt atcttgtaat ggctggtttg tttgataata tcagcaatct
    12181 tcaaaacgag aagagtctga catttttata tcgtgctcca aaaatttttc tggaaccact
    12241 tagcattcct gcaattacta caagttatcg ttctgtattt gacatttctc caagtgaggc
    12301 tgttgagatg gcgaaactta caaaggggta tccttttgct tttcagattc ttggttattt
    12361 gaagtgggag acaaatgatg atcttgaaaa actgcttcct aagtttgacg aggaattgat
    12421 catttatgct tatgagaaga tatggagtga actttctgaa cttgaccgga agattgttta
    12481 tgtaatttct acgggagttt ataaaactgg ggaaattcgt gagaaattat cgatatctcc
    12541 acagttattg aatacctatc gaaagcgatt gatggaacgt ggtgttgtga atggttctgt
    12601 tcgtggagaa ctgactttag cgctgcctcg atttgaggaa tatattgaga tgtattgtga
    12661 agtgaatctt tagtttcgct tcatctcgat gaggactctc gc
//
LOCUS       DAOMUE010000025        12660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1121_length_12660_cov_3.275526, whole genome shotgun sequence.
ACCESSION   DAOMUE010000025 DAOMUE010000000
VERSION     DAOMUE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12660
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1121_length_12660_cov_3.275526"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..185
                     /locus_tag="PLC71_02375"
     tRNA            104..185
                     /locus_tag="PLC71_02375"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:138..140,aa:Tyr,seq:gta)
     gene            198..271
                     /locus_tag="PLC71_02380"
     tRNA            198..271
                     /locus_tag="PLC71_02380"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:232..234,aa:Met,seq:cat)
     gene            295..367
                     /locus_tag="PLC71_02385"
     tRNA            295..367
                     /locus_tag="PLC71_02385"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:328..330,aa:Phe,seq:gaa)
     gene            516..1898
                     /locus_tag="PLC71_02390"
     CDS             516..1898
                     /locus_tag="PLC71_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM01527.1"
                     /translation="MTSIYNKLKQLQYKEDYPFHMPGHKRNLKIDPLLDAISQIDITE
                     ITGFDDLHHPEEMIRELMDDLKQIYGTKESYLLVNSSTAGNLAAIAALCNIGDKILVA
                     RNCHKSVYHAIELLGLDPIYIYPEIDEYGICKGITKEQIENIITKETSIKAMVLVSPT
                     YEGRVSDIEGISDVLHRNNIPLIVDEAHGAHFIYHEAFPESAANSGADIVIQSLHKTL
                     PAFTQTGLLHLCTDCVTREMMQKKLSIFQSSSPSYVLIASIEQCIHICNENRGYFQQY
                     YEKLWILREKLEELKYIKLVPTDDIGKLVFSVKDTTISGEELFEILRDNYHLEMEMSE
                     LYYVIAMTSVCDTQEGYDRLYQALKEIDSEITKKNTEYLFLENDFHQNKKMLKPEEAA
                     TKDRIQIDYDDAKDEIAAEFIFLYPPGIPLVVPGEVIDKYVIDKIRQYEQYNMKVIGL
                     DDHKIYIINR"
     gene            1911..2537
                     /gene="tmk"
                     /locus_tag="PLC71_02395"
     CDS             1911..2537
                     /gene="tmk"
                     /locus_tag="PLC71_02395"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRM01528.1"
                     /translation="MGLFLTVEGPDGAGKTTQIELLRDYLSDKGYDIIVCREPGGTPI
                     SEAVRNVILNKEFTEMGHMTELLLYAAARAQLIEEVIRPALEDGKIVICDRFVESSAV
                     YQGIARGMGIDLVYKVNQFAIGDTMPDLTIMIDLDAEAGISRKKKQAELDRMESETID
                     FHKKVVEGYRQLASLHPDRIYTVDGSLTIEEIHQKIVSQVNKKFHGKG"
     gene            2544..3536
                     /gene="holB"
                     /locus_tag="PLC71_02400"
     CDS             2544..3536
                     /gene="holB"
                     /locus_tag="PLC71_02400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRM01529.1"
                     /translation="MKSFDAIIGHKKIISHFEEAIKTGKVSHAYLLSGEDGSGKMMIA
                     KAVAKALLCEHKDGCGECAACKQVDSLNHPDVIYITHEKYEIRVDDIRKGINETIDIK
                     PYSGDYKIYIIDDADRMNAGAQNALLKTLEEPPAYAVILLLTNNKDRLLDTILSRCVS
                     MTLGSVRESEIEDYLKANTGASHADIAFAAAFSLGNIGRALHVLDTEEFKDMLNDTMN
                     VITHMKSMEIYEVVSYAKSLTKYKNEIYDFLDIIMVWYRDMLILKTTGSLNQLVFKDK
                     YRQLKDQEIYISFEGISHILDEVEKARRRLIANVNFEVAIEMLLVTIKENGKVW"
     gene            3530..4432
                     /locus_tag="PLC71_02405"
     CDS             3530..4432
                     /locus_tag="PLC71_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HRM01530.1"
                     /translation="MVEVIGVRFRTAGKIYFFNPKEFQVKSGDHVIVETARGIEFGSV
                     VSERRQVKEKCIGDELKPVLRIATKEDEERAAKNREKEKEAYKICLDKIKEHELDMKL
                     VSTEYTFDNNKVLFYFTADGRVDFRELVKDLAAVFRTRIELRQIGVRDEAKMLGGMGI
                     CGRKLCCNTFLSEFAPVSIKMAKEQNLSLNPTKISGVCGRLMCCLKNEQETYEYLNSK
                     LPNVGEKLKTKDGVVGEVQRVDVLRQKVKLIVEDENGDKEIQEYKIDDLLMRKKKPQG
                     CQGCSKGCNNKNQGCNKGHGKRKN"
     gene            4413..5147
                     /locus_tag="PLC71_02410"
     CDS             4413..5147
                     /locus_tag="PLC71_02410"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HRM01531.1"
                     /translation="MERERIDDLGINGYQIIQKEQGFCFGMDAVLLSDFAQVKNGGRV
                     LDLGTGTGILPILMEAKTKAVHLTGLEIQPEMAEMAARSVKLNHLEDKIEIVEGDIKE
                     ASAIFSHDSFDTITSNPPYMIGQHGLKNPSETKAIARHEILCTFADITAAAKKLLKNK
                     GKLFLVHRTFRLSEILCQLSKDGLEPKRIRFVHPYIDKEPNIFLLEAVKGGKSRMTVE
                     PPLIVYQKEGKYTEEIYKIYGMNPEE"
     gene            5150..5995
                     /gene="rsmI"
                     /locus_tag="PLC71_02415"
     CDS             5150..5995
                     /gene="rsmI"
                     /locus_tag="PLC71_02415"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRM01532.1"
                     /translation="MSGTLYLCATPIGNLEDITFRVINALKEVDLIAAEDTRHSIKLL
                     NHFEIKTKMTSYHEYNRVEKAKVLVKQLQEGKDIALITDAGTPGISDPGEELVRQCHE
                     AGITVTALPGACALINALIISGQPTRRFCFEAFLPSDKKERKQILDSLENETRSIIIY
                     EAPHRLVRTLEELHEVLGDRSMTLCRELTKKHESVFKSTLGEILAYHRENPPKGECVM
                     IIEGKSFQELKEESQEEFLKIPLSEHMNRYMDQGYSKKEAMKLVAKDRGVGKREIYQQ
                     LLEEE"
     gene            complement(6078..6347)
                     /locus_tag="PLC71_02420"
     CDS             complement(6078..6347)
                     /locus_tag="PLC71_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRM01533.1"
                     /translation="MKSTGIVRRLDELGRITLPIELRRNFDVNERDPLEIFVDDDKII
                     LKKYNPADIFTGEDMDDLIDYKGKKVSRKSIIELAKLAGFEITEA"
     gene            complement(6460..8430)
                     /gene="metG"
                     /locus_tag="PLC71_02425"
     CDS             complement(6460..8430)
                     /gene="metG"
                     /locus_tag="PLC71_02425"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRM01534.1"
                     /translation="MSKKPYYITTAITYTSGKPHIGNTYEIILADSIARYKRMEGYDV
                     FFQTGTDEHGQKVELKAEEKGVTPKEFVDDVAGEIKRIWDLMNTSYDRFIRTTDEDHE
                     KQVQKIFKKLYEQGDIYKGEYEGLYCTPCESFFTESQLVDGKCPDCGRPVQPAKEEAY
                     FLKLSKYADRLIEHINTHPEFIQPESRKNEMMNNFLLPGLQDLCVSRTSFSWGIPVDF
                     DEGHIVYVWLDALTNYITGIGYDCDGNSSDKFNKFWPADLHLIGKDIIRFHTIYWPII
                     LMALDLPLPKQVFGHPWLLQGDGKMSKSKGNVLYADDLVDFFGVDAVRYFVLHEMPFE
                     RDGVISWELMIERMNSDLANILGNLVNRTISMSNKYFGGIVEDKGVVGDYDDDLKAVV
                     TGTRDKVAEKMADLRVADSMTEIFNLFRRCNRYIDETMPWVLAKDEASKDRLATVLYN
                     LVEGITIGASLLSPYMPETSEKIFAQLNTSMVEFDNLATFGNYKSGTKVTEKPQILFA
                     RLDEKEVMEKAKVLMEKQAASVKAANAAVEEDTVIDIEPKDEITFDDFTKMQFQVGEI
                     IACEEVKKSKKLLCSQVKIGSQVKQIVSGIKAYYKPEDMVGKKVMVLVNLKPAKLAGV
                     LSEGMLLCAENDKGELALVTPDKDMPAGAEIC"
     gene            8546..10315
                     /locus_tag="PLC71_02430"
     CDS             8546..10315
                     /locus_tag="PLC71_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRM01535.1"
                     /translation="MEFAGRLKKIRIKLIMIGICLIFLGGYSLIQEWKDQKKNTTKKV
                     FDQYTDAFFKENISTNEITMHFFLENPEKYRLEQPKNLYPSMNQGSILNEKIKISKEI
                     EKLKKFKQKELTKKQQNTYMVLMDYLRRQKNLSMYPYYERILGKTSGQQAQILLTLSE
                     YRLKNEKDIKSYFQLLKGLDGYFDSLIEYSKEQVKRNLFLSDQSLKEVLQQIQNVIKQ
                     KENNMLVATFNLRIADIKGINAAQKKKYCRENKKLIGTKVLPAYEKLYSHLQALNGNG
                     KNENGLYYYKNGKEYYKVLVAQKTGSDKTIEEMIEISDRSIEKCLRKLIKLQKKYPNI
                     INEYRNHKKNIKIVQNPQNILNKLRQKMVKYYPKAPKVSCKIKYVHKTMEEYTSPAFY
                     MVPEIDSYKENVIYINKAQTAELYPTLAHEGYPGHLYQNVYYAARNDDPVRYLLDYPG
                     YSEGYATYVEVFSYSMMDAEGYGDIYQQMNMEMYEYNLALCSRVDLGVHYEGWKKKDV
                     RAYLRSFGMDDSQADELFQMIIENPANYLSYYIGYQEFHELLTDYKKMAGNKYSLKAY
                     HTEILDAGPCSFDILRRRIESNL"
     gene            10331..12016
                     /locus_tag="PLC71_02435"
     CDS             10331..12016
                     /locus_tag="PLC71_02435"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRM01536.1"
                     /translation="MSTKAYYTHEEIGKGKPGFSKTRSIIEAAFYGNNVVEVKDLKQA
                     YELAKNSPGTIVTDMPVYKPEAIGLDEGSKVLLFNDGSVTGRCAAARRISSEPGVDIN
                     DLDLKLMDAIYDTRWTTMYHAQVYIGLDPEFMVKAHLLIPEGEENILYSWMLNFQYMN
                     PEYNEMYKNSQPVADETDIYIFSDPQWTHEEHPLGLTYFNTDTNCAALLGMKYFGEHK
                     KGTLTIAWAIANRNGYASCHGGQKEFRLDNGEKFVASFFGLSGSGKSTLTHATHNNKY
                     EEIQVLHDDAFIINTETGASIAMEPTYFDKTADYPTGCPDNKYLLTAQNCSATRDSEG
                     KVVLVTEDIRNGNGRAIKSKLWSPNRVDKINDPVNAIFWIMKDPTIPPIVKIKGASLA
                     SVMGATLATKRSSAERLAPGVDPNRLVVEPYANPFRTYPLVNDYKKFRKLVKERNVDC
                     YIINTGDFMGKDIKPATTISAIEQVVEGTGEFKQWGPFTDIEVLEMEGYVSDFNDMNY
                     VETLKARMTDRLNFVFEKDVEDGGYNALPTQAKEALAKVIGEAHNLEVENSNN"
     gene            12082..12492
                     /locus_tag="PLC71_02440"
     CDS             12082..12492
                     /locus_tag="PLC71_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM01537.1"
                     /translation="MVVDLFHGISYEEEKAVITDEFKDISNKDLHIIDAIGIKKPKNM
                     STIAGDLNITVGTLTTAINNLLKKGYVNRKRGEKDRRVVYISLSEKGRKAYSHHREFH
                     KRMIQGLLDTLNEQEQDLLVKVMQYFHDYFREHM"
BASE COUNT         4484 a         1989 c         2622 g         3565 t
ORIGIN      
        1 cagagcagct gatttgtaat cagcaggtta ccggttcaag tccggtcaac ggcttcacac
       61 ctggtcataa ttatgttatg atttaggtgt ttaaaatata tatggttgga ttcccgagtg
      121 gccaaagggg gcagactgta aatctgttgg caccgccttc gaaggttcga atccttctcc
      181 aaccacttaa tttttatcgc gggatggagc agtctggtag ctcgtcgggc tcataacccg
      241 aaggtcgttg gttcaaatcc ggctcccgca attttttatt ttaataagta gtatgcccag
      301 atagctcagt cggtagagca gaggactgaa aatcctcgtg tcgctggttc gattccggct
      361 ctgggcatct tttttatttt ataaagaaat tcaaagagtt aactttcaga aagaatgaaa
      421 aaaccgaaag ttgactcttt tttttaattc ctattcaata ggaattaagt atgatataat
      481 aaaaatgaaa aaatatgttt acaaagggat aaaaaatgac atcaatttat aataagttga
      541 aacaactaca atacaaagaa gattatcctt ttcacatgcc aggacataaa agaaatttaa
      601 agatcgatcc attattagat gcgatttccc aaattgatat tacagagatc acaggatttg
      661 atgatcttca ccatccagaa gaaatgattc gagaattgat ggatgattta aaacagattt
      721 atggaacaaa agagagttat ctactggtaa acagcagcac tgcggggaat ttagctgcaa
      781 tagctgcatt gtgtaatatt ggagataaga tactggttgc gagaaactgt cataagtcag
      841 tctatcacgc gatagaattg ttgggacttg atccaatcta tatttatcca gagattgatg
      901 aatatggaat ctgtaaaggc attacgaagg aacagataga aaatatcata acaaaagaaa
      961 cttcaattaa ggcaatggtc cttgtttcac caacatatga aggaagagta tcagatatcg
     1021 aaggaatatc agatgttctg catcgaaata atataccatt gatcgtagat gaagctcatg
     1081 gggctcattt tatttatcat gaggcatttc cagagagtgc agcaaatagc ggagcagaca
     1141 ttgtgataca aagtctgcac aagacattgc ccgcatttac gcaaacggga ttacttcatt
     1201 tgtgcacaga ttgtgtcaca agagaaatga tgcaaaagaa attgtcaata tttcagagca
     1261 gcagcccatc ctatgtgttg atagcatcaa ttgaacaatg tatccacata tgtaatgaaa
     1321 atcgtggata ttttcaacaa tactacgaga aattgtggat actacgtgaa aaactagaag
     1381 aattaaaata tatcaaactg gtaccaacgg atgatattgg aaaacttgtg ttttcggtaa
     1441 aagacacgac aatatctgga gaagaactat ttgagatttt aagagacaat tatcatttag
     1501 agatggaaat gtcagaactt tattatgtga tcgcaatgac aagtgtgtgc gatacacaag
     1561 aagggtacga tagattgtat caggcattaa aagagatcga cagtgagatc acaaaaaaga
     1621 atacagagta tttatttttg gaaaatgatt ttcatcaaaa taaaaagatg ttaaaaccag
     1681 aagaagctgc aacaaaagat cgaatacaga ttgattatga tgatgcaaaa gatgagattg
     1741 cagcggaatt tatttttttg tatccaccag gaattccact tgttgtgcct ggagaagtga
     1801 tcgataagta tgtgatcgat aagatcagac agtatgagca atataatatg aaagtgatag
     1861 gactggatga tcataaaata tatataataa atagatgaga ggatataaat atgggattat
     1921 ttttgaccgt tgaaggacca gatggagctg gaaaaacaac acaaatagaa ttactaagag
     1981 actacttatc tgacaaaggt tatgatataa tagtatgtag agaacctggc ggaacaccga
     2041 tcagtgaggc tgtaagaaat gtgatcctta acaaagaatt tactgagatg ggacatatga
     2101 cggaattact gctttacgca gctgccagag ctcaattgat cgaagaagtc attcgtccgg
     2161 cattagaaga tggaaagatc gtaatctgtg atcgatttgt ggaatcttct gcagtctatc
     2221 agggaattgc cagaggaatg ggaattgatc ttgtttacaa agtaaatcaa tttgcaatcg
     2281 gagatacgat gccagatctg acgattatga tcgatctgga tgcagaagct ggtatttcaa
     2341 gaaagaaaaa acaggcagag ctggaccgaa tggaaagcga gacaattgat tttcataaga
     2401 aggttgttga aggatacaga caacttgcga gcctgcatcc agatagaatc tatactgttg
     2461 acggaagtct gacgatcgaa gagattcatc agaagattgt ttcacaggtc aacaaaaaat
     2521 ttcatggtaa agggtgatgt agtatgaaga gttttgatgc aattatagga cataaaaaaa
     2581 taatttctca ttttgaagaa gcaatcaaga cgggaaaagt ttcccatgcc tatttattaa
     2641 gtggcgaaga tggaagcgga aagatgatga tcgcaaaggc agttgcgaag gcacttctgt
     2701 gcgaacacaa agatggatgt ggagaatgtg cagcatgtaa gcaggtggat tcattaaatc
     2761 atccagatgt catttatatt actcatgaaa agtatgaaat cagagttgat gatattcgaa
     2821 aagggatcaa tgagacgatc gatattaaac cgtatagcgg agattataag atttatatca
     2881 ttgatgatgc ggatagaatg aatgcaggag cgcagaatgc cttattaaag acattagaag
     2941 aaccacctgc atatgcagtg attcttctat taacaaacaa taaggataga ttgttagata
     3001 cgatcctctc aagatgtgtg tccatgacat taggaagtgt cagagaaagc gaaattgaag
     3061 attatctaaa agccaataca ggggcaagtc atgcagatat cgcatttgca gcagcatttt
     3121 cacttgggaa tattggacgt gcattgcatg tactggatac agaagaattt aaagatatgc
     3181 tgaatgatac aatgaacgtg atcactcata tgaagagtat ggagatttat gaagttgtat
     3241 cgtatgcgaa gagtctgaca aaatataaga atgagattta tgatttcctt gatatcatta
     3301 tggtatggta tcgagatatg ctgattctta agacaacagg atctttaaat caacttgtat
     3361 ttaaagataa ataccgtcag ctaaaagatc aggagattta tatttcattt gagggaatca
     3421 gtcatattct tgatgaagta gaaaaggcta gaaggcgact gatcgcgaat gttaattttg
     3481 aagtggcaat cgaaatgctg cttgtgacga taaaggagaa tggtaaagta tggtagaagt
     3541 aattggagtg cgatttcgta ctgcaggaaa gatatatttc tttaatccaa aggaatttca
     3601 ggtaaagtca ggagaccacg tgattgtgga aactgccaga ggaattgaat ttggatcagt
     3661 cgtttctgag agaagacagg taaaggaaaa atgcatcgga gatgaattaa aacctgtttt
     3721 aagaatcgca accaaagaag atgaagaaag ggcagcgaag aatagagaga aagaaaaaga
     3781 agcatataag atctgccttg ataagatcaa ggagcatgag cttgatatga agcttgtatc
     3841 aacagaatat acatttgata ataataaagt attattttac tttacggcag atggacgagt
     3901 tgattttcgt gaacttgtaa aggatctggc agcagtgttc aggacaagaa ttgaattacg
     3961 acagatcggc gtcagagatg aagccaagat gcttggtggt atgggaatct gtggaagaaa
     4021 attatgctgt aatacatttt tatctgagtt tgcacctgtt tctatcaaga tggccaaaga
     4081 gcagaatctg tcactgaatc cgacaaagat ttcaggagtc tgcggaagat tgatgtgctg
     4141 tcttaagaat gaacaggaaa catatgagta tttaaacagt aagcttccaa atgttggtga
     4201 gaaattaaag acaaaagatg gcgtagttgg agaagttcag agagtagatg tacttcgaca
     4261 gaaggtaaaa ctgatcgtgg aagatgagaa tggagataaa gaaatccagg aatataagat
     4321 tgatgatctt ctgatgagaa agaaaaagcc acaaggatgt cagggatgca gcaaaggatg
     4381 caataataag aatcaaggat gtaataaagg acatggaaag agaaagaatt gatgatcttg
     4441 ggatcaatgg ttatcagatc atccagaaag aacagggatt ttgttttgga atggatgcgg
     4501 ttcttttatc agattttgca caagttaaga atggtgggag agttctagac ctcggtacgg
     4561 ggactggaat cctcccaatt ttgatggagg caaagacgaa ggctgttcat cttacagggc
     4621 ttgaaataca gccagagatg gcggagatgg cggcaagaag tgtcaaatta aatcatttag
     4681 aagataagat agagatcgta gagggtgata tcaaagaggc ttctgcgatt ttttcgcatg
     4741 atagttttga cacgatcaca tcaaatccac catatatgat cggacagcat ggacttaaga
     4801 atccgtcgga gacgaaagca atcgcaagac atgagatact ttgtacattt gcggatatca
     4861 cggcagcagc taaaaagctt ttaaagaata aaggaaagct gtttctggta cacagaacat
     4921 tcagattgtc agagatttta tgccagctgt ctaaggatgg attagagcca aaaagaatcc
     4981 gttttgtgca tccatacatt gataaagaac cgaatatatt tttattagaa gcagtaaaag
     5041 gcggaaagtc aagaatgaca gttgagcctc cattgatcgt ttatcaaaaa gaaggaaaat
     5101 acacagaaga aatttataag atttatggaa tgaatccgga ggaataagca tgagtggaac
     5161 attatatttg tgtgcgacac cgattggtaa tctggaagac attacttttc gtgtgataaa
     5221 cgcattaaaa gaagttgatt tgatcgcagc ggaagacaca aggcacagta tcaaattatt
     5281 aaatcatttt gaaattaaga caaagatgac aagctatcat gagtataatc gtgtggagaa
     5341 agcaaaagtt cttgtaaagc agttgcagga aggaaaagat atcgcactca ttacagatgc
     5401 tgggacaccg ggaatttcag atccaggaga agaattggta agacagtgcc atgaagcagg
     5461 aatcacagtt actgcattac cgggagcatg tgcgttgatc aatgcactga tcatctctgg
     5521 gcagccaaca agaagatttt gttttgaagc atttcttcca tcagataaaa aagaaagaaa
     5581 acagattctt gattctctgg aaaatgagac aagatctatt atcatttatg aagcaccaca
     5641 tcgtttagta aggactttgg aagaactaca tgaagtgctc ggagacagat ccatgacatt
     5701 atgcagagaa ctcacaaaga agcatgagag cgtttttaaa tcaacacttg gggaaatcct
     5761 tgcctatcat agagaaaatc caccaaaagg cgaatgtgtg atgatcatag aaggaaaatc
     5821 atttcaagaa ttaaaagaag agagtcagga agaatttcta aagattccat tgtcagagca
     5881 tatgaaccgt tatatggatc agggatattc aaagaaagaa gcgatgaaac tggtagccaa
     5941 agatcgaggt gttggtaaga gagagattta tcagcagttg ttggaagagg aataaaaata
     6001 tatcaacaaa aaaggtatgg gaaaataata tcaattccca taccttttgt ttgtctttat
     6061 tgaaatgatc caaaaaatta cgcttcagta atttcgaatc cagcaagttt agctaattca
     6121 atgatagatt ttcttgagac tttctttcct ttgtagtcaa tcagatcatc catatcttct
     6181 ccagtaaaaa tatctgcagg attatatttc tttaaaataa tcttatcgtc atctacaaat
     6241 atttcaagag gatcgcgttc gttcacatca aagttacgac gtaattcgat cggtaaagta
     6301 attcttccaa gttcgtcaag ccttcttaca atgcctgtac ttttcatgtg taaacctcct
     6361 taatgtctta caatcatttt atatcttaaa ccatagcata ccaaaatctg gttgtcaatt
     6421 catttgttta cttataaact agagaataaa taagtataat taacagattt cagcacctgc
     6481 tggcatatct ttatctggtg ttacaagagc taattcaccc ttatcatttt cagcacaaag
     6541 taacattcct tcagagagaa ctcctgctaa tttggctggt tttaagttca caagtaccat
     6601 aactttctta ccgaccatat cttctggttt ataataagct ttgataccag aaacaatctg
     6661 ttttacctgg cttccgatct taacctgaga gcaaagaagt ttcttggatt tcttcacttc
     6721 ttcacaggca atgatctcac caacctggaa ctgcatctta gtgaaatcat caaaagtgat
     6781 ctcatctttt ggttcaatgt cgatgacagt atcttcttct acagcagcgt ttgcagcttt
     6841 gacagaagca gcctgtttct ccattaagac tttggctttt tccataactt ctttctcatc
     6901 taatcttgca aataagatct gaggtttttc agttactttt gtaccactct tgtagtttcc
     6961 aaatgttgca aggttatcaa attcaaccat agatgtattt aactgagcaa agatcttctc
     7021 agatgtctca ggcatatatg gtgataataa agatgcacca attgtgattc cttcaacaag
     7081 gttgtataaa acagtagcca gacggtcttt tgaagcttcg tcttttgcta atacccaagg
     7141 catagtctca tcaatataac ggttgcagcg acggaataag ttaaagatct cagtcattga
     7201 atctgcaaca cgaaggtcag ccatcttctc agcaacttta tctcttgtac ctgtcacaac
     7261 ggctttcaga tcatcatcat aatctcctac aacacccttg tcttctacga ttcctccaaa
     7321 atatttgttg gacatagaaa tcgtacggtt tacaaggtta ccaagaatat ttgcaaggtc
     7381 agagttcata cgctcgatca tcaattccca tgagatcaca ccatcacgtt cgaaaggcat
     7441 ttcatgaaga acgaaataac gcactgcgtc aactccgaag aagtcaacca gatcatctgc
     7501 gtataaaacg ttacctttgg atttactcat cttaccatcg ccctgaagca gccaaggatg
     7561 gccaaatacc tgtttaggaa gtggaagatc tagtgccatc aggatgattg gccagtaaat
     7621 tgtatgaaaa cggatgatat ctttaccgat caggtgaaga tctgcaggcc agaacttgtt
     7681 aaacttgtct gaactgtttc catcacagtc ataaccgata cctgtgatat agtttgttaa
     7741 tgcatctaac catacataaa cgatgtgtcc ttcatcaaag tcaactggga ttccccatga
     7801 aaatgatgtt cttgaaacac acagatcctg aagtccagga agaaggaagt tgttcatcat
     7861 ctcattctta cgggattctg gctgaataaa ttcaggatgt gtattgatgt gttcgatcag
     7921 acggtctgca tatttgctta attttaagaa ataagcttct tcttttgcag gttgtactgg
     7981 acgtccgcag tcaggacatt taccatctac taactggctc tctgtgaaga aagattcaca
     8041 aggtgtacag tataaacctt cgtattcacc tttgtagata tctccctgtt catataattt
     8101 cttaaaaatc ttctgaacct gtttctcgtg atcttcatca gttgttctga taaaacggtc
     8161 ataagaagtg ttcataagat cccagattct cttgatttct ccggcaacat catcaacgaa
     8221 ttcttttggt gttacccctt tttcttctgc ttttaattct actttctggc catgttcgtc
     8281 agttcctgtc tggaaaaata cgtcatagcc ttccattctt ttatatcgag caatactgtc
     8341 tgccaggatg atctcatatg tgttaccaat atgaggtttt ccggaagtat aagtaattgc
     8401 agtcgtaata taatatggtt tcttactcat aatgatcctc cgattttctg tttctatcct
     8461 tataattata gtacatcttg tgcggatagt ctattttttt tctttaaaaa ataagatatt
     8521 aacaagtgtt tatttggagg tagaaatgga atttgcaggg agattgaaaa agatcagaat
     8581 aaagttgatc atgattggaa tttgtctgat atttctagga ggatatagtc tgatccaaga
     8641 atggaaagat caaaaaaaga atacaacaaa aaaagtattt gatcagtata cggatgcgtt
     8701 ttttaaagaa aatatatcaa caaatgagat cacgatgcat ttctttttag aaaatccaga
     8761 aaaatataga ttagaacaac cgaaaaatct ttatccatcc atgaatcagg gaagtatctt
     8821 gaatgaaaaa attaaaattt caaaagaaat tgagaagtta aaaaaattta aacaaaagga
     8881 attgacgaaa aaacagcaaa atacatatat ggttttgatg gattatttaa gaagacagaa
     8941 aaatctttcg atgtatccgt attatgaaag aatccttggg aaaacatctg ggcagcaggc
     9001 acagattctt ctgacattaa gtgaatatcg tctgaaaaat gagaaagata tcaaaagcta
     9061 ttttcaatta ttgaagggac tggatggata ttttgattca ctgatcgaat acagcaagga
     9121 acaggtaaag agaaatttat tcctatctga tcagtcttta aaagaagtat tgcagcagat
     9181 tcagaatgtc ataaagcaaa aagaaaacaa tatgttggtt gcaacattta atttaagaat
     9241 tgcagatatt aaaggaatta atgcagctca gaaaaagaaa tactgcagag aaaataagaa
     9301 attgatcggt acaaaggtgc tgcctgctta tgaaaaatta tattcccatt tgcaggcatt
     9361 aaatggaaat gggaaaaatg aaaatggtct gtattattat aaaaatggaa aagaatatta
     9421 taaagtattg gttgcacaga aaacaggatc agataaaacg atagaagaaa tgatcgaaat
     9481 cagtgatcga tccattgaaa aatgccttcg aaaacttata aaattgcaaa aaaagtatcc
     9541 aaatatcata aatgaatata gaaatcataa aaaaaatata aaaattgtgc aaaatccaca
     9601 aaatatactt aacaaactaa ggcaaaagat ggtaaaatat tatccaaagg caccaaaagt
     9661 tagctgtaaa ataaaatatg tgcacaaaac catggaagaa tataccagtc ctgcgttcta
     9721 tatggtacca gagattgatt cttacaagga gaatgtaatt tacattaata aggcacagac
     9781 tgcagagtta tatccaactt tggcacatga aggataccca ggacatttat accagaatgt
     9841 atattatgcg gcaaggaacg atgatccagt gcgctatctg cttgattatc ctggatattc
     9901 agaaggatat gcaacgtatg tagaagtttt tagttacagt atgatggatg ctgaaggata
     9961 tggcgatatt tatcaacaga tgaatatgga gatgtacgag tacaatctgg cactttgttc
    10021 aagagttgat cttggagttc attatgaagg atggaagaag aaagatgtca gggcgtattt
    10081 aagatcattt ggaatggatg attctcaggc agatgaatta tttcagatga ttatcgagaa
    10141 tcctgcaaat tatttatctt attatatcgg ttatcaggag tttcatgagc ttttgactga
    10201 ttataagaaa atggcaggga ataaatacag cttaaaagcg taccacacag agattttaga
    10261 tgcaggccca tgtagttttg acattttgag aagacgcatc gaaagcaacc tatgaatgga
    10321 ggagtaactt atgtcaacaa aagcttatta cacacatgaa gaaatcggaa aaggaaagcc
    10381 tggattttca aaaacaagat ccattattga agcagcattc tatggaaata acgttgtaga
    10441 agtaaaagat ttaaagcagg cttatgaatt agcaaagaat tcgcctggaa cgatcgttac
    10501 agatatgcca gtatataaac cagaagcgat tggtttagat gaggggtcca aagttctatt
    10561 atttaatgat ggatcagtga ctggacgctg tgcagccgca agaagaattt ccagtgaacc
    10621 tggtgtcgat ataaatgatc tggatttaaa attaatggat gcgatctatg atacaagatg
    10681 gacaacaatg taccatgcac aggtatatat cggattagat cctgaattta tggtaaaagc
    10741 acatctttta attccagaag gtgaagaaaa tattttatat tcctggatgt taaatttcca
    10801 gtatatgaat cctgaataca atgaaatgta taagaattca cagccagttg cagatgagac
    10861 agatatctat attttctcag atccacagtg gacacatgaa gaacatccat taggattaac
    10921 atattttaat acagacacaa actgtgctgc acttcttggt atgaaatact ttggggaaca
    10981 taagaaaggt acattaacaa ttgcatgggc gatcgcaaat cgtaacggat atgcttcctg
    11041 ccatggtgga cagaaagaat tcagattaga taatggagag aaattcgtag catctttctt
    11101 cggattatca ggatcaggaa aatccacatt aacacatgca acacataata ataaatacga
    11161 agagattcag gttctgcatg atgatgcatt tatcatcaat acagaaacag gtgcatctat
    11221 tgcaatggaa ccaacatatt ttgataaaac agcggactat ccaacaggat gcccagataa
    11281 taaatactta ttaacagcac agaattgctc tgcaacaaga gatagcgaag gaaaagtcgt
    11341 tcttgtcaca gaagatatcc gtaacggaaa tggtcgtgcg atcaagtcca aattatggtc
    11401 accaaaccgt gtagataaga tcaatgaccc agtaaatgca atcttctgga tcatgaaaga
    11461 cccaacaatc ccaccaattg ttaagatcaa aggagcttct ttagcatctg ttatgggagc
    11521 aacacttgca acaaaacgaa gcagtgcgga acgtctggca cctggagtag atccaaaccg
    11581 tttggttgtg gaaccatatg caaacccatt tagaacatac cctcttgtaa atgactacaa
    11641 gaagtttaga aaacttgtaa aagaaagaaa tgtagactgc tatatcatta atacaggaga
    11701 cttcatgggt aaagatatca aacctgcaac aacaatttcc gcaattgagc aggttgtaga
    11761 aggaactgga gaattcaaac agtggggacc atttacagat atcgaagtgc ttgagatgga
    11821 aggatatgtt tcagatttca atgatatgaa ttatgtggaa acattaaaag caagaatgac
    11881 agacagactg aactttgtct tcgaaaaaga cgtagaagat ggtggataca atgcacttcc
    11941 tacacaggca aaagaagctt tagcaaaagt tattggagaa gcacacaatt tagaagtgga
    12001 aaattctaat aattaaaggt ataatactct taaatcgttg tttaggaggt aaattgattg
    12061 gataaagatc gtttaaataa tatggtggta gacttattcc acggaatttc atatgaagaa
    12121 gagaaagccg tgatcacaga tgagtttaag gatatttcga acaaagatct gcatattatt
    12181 gatgcgatcg gaatcaagaa accgaagaat atgtcaacga ttgcaggaga tttaaacatt
    12241 acggttggta cacttacaac cgctatcaat aacctcttga agaaaggata tgtaaatcgc
    12301 aaacgcggag agaaagatcg tagagttgtt tatatatctc tttctgagaa ggggaggaaa
    12361 gcatacagtc atcacagaga atttcataaa cgtatgatcc agggactgct tgatacgctt
    12421 aatgagcagg aacaggatct gttggtgaag gttatgcagt attttcatga ttactttcgt
    12481 gaacatatgt aaaatttatt tgttagtttg atagctgtag tctttgtgta tctgagaaaa
    12541 acgaaataaa agaataaaag atccagacca gaaattttgc tcgccgtttt gaacggtcgc
    12601 ttatacgcaa attttccttg cataactcac tcgctacgcg agttcaaaca atgcaccaaa
//
LOCUS       DAOMUE010000026        12194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1202_length_12194_cov_2.747838, whole genome shotgun sequence.
ACCESSION   DAOMUE010000026 DAOMUE010000000
VERSION     DAOMUE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 12194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12194
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1202_length_12194_cov_2.747838"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="PLC71_02445"
     CDS             complement(<1..593)
                     /locus_tag="PLC71_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01538.1"
                     /translation="MSQSQYYVTHYFNAQNASSFMAVSLIGLIVVLAIVLLTAKLQKH
                     YKINIENPTEVISWIESFYEMVFSSTSVLIFLSLYYVLDERIPQAAYFWNKYQDILLM
                     VFLVCSVFLTFWFDRIFVHLKMISSEQKASVRLISAFYIILILLYIKFIYNDSNYDSL
                     IFYFVMLAIGRFVYFDSTLDYIKESLKSVVKYLPLLI"
     gene            838..1710
                     /locus_tag="PLC71_02450"
     CDS             838..1710
                     /locus_tag="PLC71_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM01539.1"
                     /translation="MKKSVRITAMIFAIFAILIAVEIIISYKCLTVTDYKIKSDKIKE
                     TTKIVLISDLHNSQFGSKNKRLVDKIQKQDPDLILMDGDMLNEDGKNAQTAVELISAL
                     KKTAPVYYALGNHEIAYRQRRDKNLYQKLQKAGAKVVEKEYEDIKVRSNKIRIGGLYE
                     YAFAVDGAGNMVKKNIPSKVRDFLMDYENTDAFKIMLSHRPDSFVFGQAADTWKIDLV
                     VSGHVHGGQVRIPGKGGLYGGDQGWFPEYTDGIHHFKTVNHMIITRGLGSDKEKLPRF
                     HNIPEIVVIRLEKR"
     gene            1729..2583
                     /locus_tag="PLC71_02455"
     CDS             1729..2583
                     /locus_tag="PLC71_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01540.1"
                     /translation="MKKYQIADFIFGIHMEESLEIPENFKKFSIDTDEEVQVEYFLEV
                     VDKLPELDGECVSQRIDLKVYKKDNLEYRRMNFMGMEESYGQYEEISQDQVKLYLKRE
                     FVHMINVDTVFVSLFAMEKRMLAKDAMVLHCSVLKVKSGVILFSGPSGIGKSTQAGLW
                     TKYRKARVINGDRTLLKKENGRWMSLGWPICGSSEICYNEAYPVKAVIFLGQAEENDG
                     RRLRYFDAIKAMISQITVNGWNPQFVEKIWNLTEDFAAQIPVFEYGCNMEEAAVDTLE
                     NLLLDILE"
     gene            2598..3836
                     /locus_tag="PLC71_02460"
     CDS             2598..3836
                     /locus_tag="PLC71_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRM01541.1"
                     /translation="MNLTNVFIDILRSAMTQTSCDMIEGLSTQDWEKIYDISQKQQLA
                     PMIYQQIFSNESFQNSDPGFQQFWKGDTIDQAGNQARKSILFLMLFDKMRQNGLTPLI
                     VKGIVCRDLYPNPDLRTSNDEDLYIPRDQFGKMDEFLQAEGFMREELIKDKVYQEVPY
                     QNPRNGLYFELHMDLFPQESGAYGHFNQLFEDAFDTCMETDIQGSKVLTLNPKQHFLY
                     LVCHSLKHFLHSGFGVRQACDLLYFAKKYHDQLDFHEIRNIMKEYHMDSFAMNVLDIG
                     VCYLGFTWEELGLSKPSDVEIDCTALLDDMLDGGIFGQNDMNRVHSANITLNAAENES
                     VNAASGILASLFPGKEYIKTNYPYSRNYPFLLPVAYLHRIFKYLTHRNTDAINTGEKS
                     SAQIGMERVKLLEKYKIVEK"
     gene            3865..4740
                     /locus_tag="PLC71_02465"
     CDS             3865..4740
                     /locus_tag="PLC71_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01542.1"
                     /translation="MLDQEKFFNTYKVQEAFEDSGLSWDTLEKIYEDYTRRLPEMKKI
                     ADRLQDEISKVIDFHVHSIHNRCKDPEHLIEKIIRKVGVEKRQKYKNINERNYLRIVR
                     DLMGIRILILSKEEWRTVHDFLLKVDEDSRYDMHMAEMPRAYIRYGDRDIFNYTIHKE
                     YTDKGYRSQHYIFKYGNYYFEVQVRTIAEEVYAEFDHYVKYPYRENNHFLKRYTSVVA
                     ELLNSVDEIISTCFQFGEAGWLDIEKYFAQDSYSDLQRMSKEPHKREQEQIPRANDGV
                     IDAQSYMNRMILRKG"
     gene            4746..4997
                     /locus_tag="PLC71_02470"
     CDS             4746..4997
                     /locus_tag="PLC71_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRM01543.1"
                     /translation="MERYDDGFAEERQRIERLVEEIIAGEHKIDKLPNSSAGFSGAAQ
                     AEYFSKARYHLESDVSNLIVNHKQQLDELQLLKMIKGQE"
     gene            4994..5524
                     /locus_tag="PLC71_02475"
     CDS             4994..5524
                     /locus_tag="PLC71_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01544.1"
                     /translation="MIEHNLFEMENYRELIDEVGVDKFRERFEELQKTAMEFIEMAGF
                     SETSYCNERILMQVILDYFMDVMRLKEFHSIERIRTEKLFAYTISWIVRRKPIQFRDY
                     SEEERDIFINERFAAYLLINECLMCGTKHFVQEAYAEKLVEYTEMLLYYFKYRQCDPK
                     TLELLIESFKMGGLVH"
     gene            5544..7016
                     /locus_tag="PLC71_02480"
     CDS             5544..7016
                     /locus_tag="PLC71_02480"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRM01545.1"
                     /translation="MGILVFDVGTSSMKGALLNDKAEILCQFHRKYHPTFYSSVKVTQ
                     DPEIWRKALYDIARDVGDWCKDQNEEVEMISLTAQRTSIIPVDHKGDPLCDAVMWQDK
                     RHIEVCTRLSAMNGQIIRKSGTMVNPVFSGSKMAWLKETQPEIYKKADRIFVVADYLL
                     YHMTGQRKLDRTYASRSHLMNLRTGRWDSVLLDIFGIEKNKLCDLIDPGELHGYTNEK
                     FAASTRLKTGIPVYSAGGDQQCSALGMGIYEEGDMEVTSGTGAFIIGIAEKVPIVLLG
                     NPIINYSAVKGKYIVEASILTCSAAFDWFCNNFYKEVNGDVYQKIDQEISQSPPGANG
                     CMLLPFFQGRATPQWNPKATASFTNVTLSTTRGDMVRALLEGIAYEIRNNLDIVEGQI
                     GKADQIHIGGGLSNSHEFNQIQADVYGRKLIHYQHREAGIIGAWTSTMVESGKVSDHE
                     EALERAGRNNSLQIYEPVEKNIEKYQELRADMNHLYQKIY"
     gene            7329..7778
                     /locus_tag="PLC71_02485"
     CDS             7329..7778
                     /locus_tag="PLC71_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM01546.1"
                     /translation="MSEVIEARNIKLNVDASDWRDSMVKSGQLLVDSDYITKDYIDLT
                     IKCVEENGPYIVIIPGLALSHSRPDVSVNKTGLSLITLSKPVCFDCDNDPVDIVLTLA
                     ATDDTSHLQKLQSMAEFISDEENIEFIKNAKSAEEVAKAINEFEPEE"
     gene            7794..9233
                     /locus_tag="PLC71_02490"
     CDS             7794..9233
                     /locus_tag="PLC71_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HRM01547.1"
                     /translation="MEILNTVFTFVSNNILTKAEFFVGLIVLVGYILLGRKWYETLAG
                     FLKAVVGYMILNVGSAGLINTFRPILAGLNQKFHLNAVVIDPYFGLNAVNAALENIGL
                     SAAWTMTSLLLAFIWNIILLIFRKFTKLRTLLLTGHIMVQQATTVTWIVFLFIPGLRN
                     IWGAVAVGILVGTYQAVFSNLTVEPTDRLTDGEGGFAIGHQQMFAIWLADKIAPKIGK
                     KEDSIENIKLPGFLQMFSDNVVSTSVLMFIFFGIIMLVLGEDFMRSLDDTFSQTTAFG
                     FYIFSKALSFTVYLNVLQAGVRMFVAELTESFKGISDKLLPGAIPAVDCAVAYGFAPA
                     NAVTVGFFCGALGQFLAIAGLLIFHSPVMIIAGFVPLFFDNASIAVYANHRGGVKAAM
                     ILPFISGIIQVLGGAVCAYVLQLVAFGGWHGNFDFSTIFLAVSYIVKYLKVPGVILCV
                     IAMLVIPQIQYAKSDKEKYFTVGQEDDEY"
     gene            9298..9591
                     /locus_tag="PLC71_02495"
     CDS             9298..9591
                     /locus_tag="PLC71_02495"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM01548.1"
                     /translation="MKIKAIAACGNGMGSSQMIKMKINKVFKRLGIDAEIGHMSVGEA
                     KGVANNYDMIFVSETFTSQFRTGKDTKVIGLKNLLSEKEIEAKIREALDMPAE"
     gene            9607..10479
                     /locus_tag="PLC71_02500"
     CDS             9607..10479
                     /locus_tag="PLC71_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HRM01549.1"
                     /translation="MYDIKKRPLGIYEKALPNDFTWLEKMQAAKQAGFDYIEISIDES
                     DERLARLDWSDEQIEEIRSYMKETGIIIPSMCLSGHRRFPFGSKNKEIRDKAFEIMEK
                     GIILAQKLGVRVIQLATYDVYYEESDEETKALFLEGMKKSVEMASHAGVILAMEIMDT
                     PFVGTILRAMHYIKEIRSPYFKIYPDMGNLTQFSADVESEFELGISETVAIHVKETNP
                     GVFKCVPFGEGTVRFTDIFKKLKDLNYTGMFLIEMWADNTKKQTMEEAVETIRQARLF
                     VEDKMEQAGIKVPA"
     gene            10490..11317
                     /locus_tag="PLC71_02505"
     CDS             10490..11317
                     /locus_tag="PLC71_02505"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM01550.1"
                     /translation="MYRMVVTDLDGTLLNNKKQVSEANVKAIQNLKDQNITFVMATGR
                     SDVMTRAYTKQLKNVDIVIGCDGAVIRNIRTGEILCENHLRSETCHTTFEICKKYGLQ
                     YYVFAKDELVSDDPQNERFLVHQKFNQTVEEDEEIPMQIVDDLNEYVNDHIIYKIVVS
                     HNDTSYLDKVAEVIKKETDADAIRSGKKVLAVKARGVSKAEAIKKLAQKLGILIKDII
                     AFGDEVNDIEMLTLVGLGIAMENADDVVKEAADQIAGRNDQDGVGKELEKIFGNAGK"
     gene            11334..12026
                     /locus_tag="PLC71_02510"
     CDS             11334..12026
                     /locus_tag="PLC71_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HRM01551.1"
                     /translation="MLDELKQQVYEANMELPAKGLVTYTWGNVSGIDRERGLFVIKPS
                     GVDYEKLTPDMLVVMDLNGEKVEGDLNPSSDTPTHIELYKAFPEVGGIVHTHSPWATS
                     WAQAGRSIPCYGTTHADYFFGDIPCARSLTADEINGEYEKNTGIVIAETFEGKNPIYV
                     PGVLCTNHGPFSWGADAAEAVHNAVVMEEVAKMAYRCEHLKKDIEEAPQVLQDKHFFR
                     KHGENAYYGNDL"
     gene            12071..>12194
                     /locus_tag="PLC71_02515"
     CDS             12071..>12194
                     /locus_tag="PLC71_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HRM01552.1"
                     /translation="MSKISEMTRESWIESTFPEWGTWLVEDIENEVVAPGNVAMW"
BASE COUNT         4282 a         1741 c         2787 g         3384 t
ORIGIN      
        1 aaaatcagca aaggtaggta tttcactaca cttttcaaag attccttgat gtaatcaagc
       61 gttgaatcaa aatagacaaa acgaccgatc gcaagcatta caaaataaaa gatcaaagaa
      121 tcataatttg aatcgttata aataaatttt atgtacaaca agatcagaat gatataaaag
      181 gcagagatca gtcgtacgga ggctttttgt tcggatgaga tcattttcag atgaacaaag
      241 atccggtcaa accagaatgt taaaaataca gaacatacta gaaataccat caacaaaata
      301 tcttgatatt tattccagaa ataagccgcc tgagggatcc tttcgtcgag gacataataa
      361 agagaaagga agatcagtac ggaggtgctt gaaaatacca tttcataaaa gctttcgatc
      421 cagctgataa cttctgttgg attttcaata ttgatcttat aatgtttttg aagctttgcg
      481 gtaagtaaaa cgatggcgag gacaacaatt aggccgatca aagaaacagc catgaagctg
      541 ctggcatttt gtgcattaaa ataatgtgta acataatatt gtgattgtga catatataac
      601 cccttcttta aactaaaata atgacatgaa aatcaaataa tctcactaaa cacatatttt
      661 aacacctttt tatataaatt caatggatac tatatttcat cataaaaact taagaaagtt
      721 ttttggacaa cagaaaggca gtggatttgt gtgagaaatt tatctataga cttgtgagca
      781 gagactggac aatgttataa tataaatagc acattttttg aaagtggtga gagaagattg
      841 aagaaatcag taaggatcac agcaatgata tttgccattt ttgctatatt gatagcggta
      901 gaaattatca tatcttataa atgtctgact gttacggatt acaagattaa gtctgataag
      961 atcaaggaaa cgaccaagat cgtattaata tcggatcttc ataatagtca gtttggaagc
     1021 aagaataagc gcttagtgga taaaattcaa aaacaagatc cagatcttat cttgatggat
     1081 ggagatatgt tgaatgaaga tgggaagaat gcacagacag cagtagaact tataagtgca
     1141 ttgaaaaaaa cagcacctgt atattacgca ttggggaatc atgagattgc gtatagacag
     1201 cgcagggata agaatctata tcagaagtta cagaaagcag gtgcgaaggt tgtagagaaa
     1261 gaatatgagg atataaaggt aagaagcaac aagatccgga tcggaggcct ttatgagtat
     1321 gcatttgcag tagatggggc tggaaatatg gttaagaaaa atattccgtc caaagtaaga
     1381 gattttttga tggattatga gaacacagat gcatttaaga tcatgttgtc tcacagacca
     1441 gatagttttg tttttgggca ggcagcagat acatggaaga tcgatctggt agtcagtggg
     1501 catgtacatg gtggacaggt aagaattcca ggtaaaggag gtctttacgg aggagatcag
     1561 ggatggtttc ctgaatatac ggatggaatc catcatttta agacagtgaa tcatatgatc
     1621 attacaagag gacttggaag tgataaggaa aaactaccaa gatttcacaa tattccagag
     1681 attgtagtga tccgacttga aaaacgttag aacaaagagg atgagaggat gaagaaatat
     1741 cagattgcag attttatttt tggaattcat atggaagaat cgttagaaat tccagagaat
     1801 tttaaaaaat tttcgattga taccgatgaa gaagtacaag tggaatattt ccttgaggtt
     1861 gtggataaat taccagagtt ggatggggaa tgtgtatcac aaagaattga tctgaaagta
     1921 tataagaaag acaatttgga ataccgccgg atgaatttta tgggaatgga agaatcctat
     1981 ggacaatatg aagaaatttc acaagatcag gtaaaattgt acttaaaacg ggaatttgtg
     2041 catatgataa atgttgatac agtctttgta tctctttttg cgatggaaaa aagaatgttg
     2101 gcaaaggatg cgatggtatt gcattgttct gtactcaaag tgaaatcagg agtgatctta
     2161 tttagcggac caagtggaat tggtaagtca acgcaggcag gattgtggac gaagtatcgg
     2221 aaagcaagag ttatcaatgg tgatagaact ttattaaaaa aagagaatgg cagatggatg
     2281 tctcttggat ggccgatctg tggttcttca gagatctgtt ataatgaagc ttatcctgtg
     2341 aaagcagtca tttttcttgg acaagcagag gagaatgatg gaaggagact tcgttatttt
     2401 gatgcgatca aggcaatgat cagtcagatc acagtgaatg gatggaatcc gcagtttgtg
     2461 gagaagatat ggaatcttac agaagatttc gcggctcaga tcccggtgtt tgagtatgga
     2521 tgtaacatgg aagaagcggc agtagatact ttagagaatc tgcttttgga tattttagaa
     2581 taaaggagta tgtgaagatg aacttaacca atgtatttat agatatatta agaagtgcta
     2641 tgacgcagac ttcctgtgat atgatagagg gactttctac acaagactgg gagaagatct
     2701 atgatattag tcagaaacag caactggcac cgatgatcta tcagcagatt ttttcaaatg
     2761 aatcatttca aaatagtgat ccagggtttc agcagttctg gaagggagat acgatcgatc
     2821 aggcaggaaa tcaggcaaga aaaagtattc tgtttttgat gttgtttgat aaaatgcgcc
     2881 agaatggatt gactccattg atcgtgaaag gaattgtttg cagagatcta tatccgaatc
     2941 cagatcttcg aacatccaat gatgaagatc tttatatccc aagagatcag tttgggaaga
     3001 tggatgaatt tttgcaggca gagggattta tgagagaaga actgatcaag gataaagttt
     3061 atcaggaagt tccatatcag aatccaagga atggattata ttttgaactg catatggacc
     3121 tgtttcctca ggaatccgga gcgtatgggc attttaatca attatttgaa gatgcgtttg
     3181 atacttgtat ggaaacagat attcaaggga gtaaagtatt gacgctgaat ccaaaacagc
     3241 attttttata tcttgtatgc cacagtctga aacattttct tcatagtgga tttggagtca
     3301 gacaggcatg tgatctatta tactttgcga agaaatacca cgatcagtta gactttcatg
     3361 agatcaggaa tatcatgaaa gaatatcata tggattcttt tgctatgaat gtattggata
     3421 ttggagtctg ctatcttgga tttacttggg aggagctagg gctttcaaaa ccgtctgatg
     3481 tagaaattga ctgcacagca ttattggatg atatgttaga tggtggtatt tttggacaaa
     3541 atgatatgaa tcgtgtacat agtgcaaata ttacgttgaa tgcagcagag aatgaatcag
     3601 tcaatgcagc ttccgggatc ctggcttctt tatttccagg gaaggaatat atcaagacga
     3661 actatccata ttcaaggaac tatccgtttt tactgccagt ggcgtattta cacaggatat
     3721 ttaaatactt aactcatcgt aacacagatg cgatcaatac aggagaaaaa agcagtgcac
     3781 agattgggat ggaacgtgtc aaattattag agaaatataa gattgtagag aaataaatat
     3841 aaaagtgaaa agaggtgatt cctcatgctg gatcaggaga aattttttaa cacatacaaa
     3901 gtacaggaag catttgagga ttctggactt tcctgggata cgcttgagaa gatttatgag
     3961 gattatacga gacgtttacc agagatgaaa aagattgcag atcgtttaca ggatgagata
     4021 tcaaaagtaa tagattttca tgtacattca attcataacc gatgtaagga tccagagcat
     4081 ttgattgaga agatcatacg gaaagttgga gttgaaaaac gtcagaaata taaaaatatt
     4141 aatgaacgga attatctgag gatcgtgcgt gatcttatgg gtatccgaat tttgatctta
     4201 tcaaaagaag aatggcgtac ggtacatgac tttttgctga aagttgatga agattccaga
     4261 tatgatatgc atatggcgga gatgccaagg gcttatatcc gttatgggga tcgtgacatt
     4321 tttaattata cgattcataa agaatataca gataagggat atcgttcaca gcattatatt
     4381 tttaaatatg ggaattatta ttttgaagta caggtacgaa cgattgctga agaagtatac
     4441 gcagaatttg accattatgt aaagtaccca tatcgtgaga ataatcattt tctaaagcgg
     4501 tatacaagtg ttgtggcaga attgttgaat tctgtggatg agatcatttc gacatgtttt
     4561 cagtttggag aagcaggctg gcttgacatt gagaaatatt ttgcacaaga cagctacagt
     4621 gacttgcaaa gaatgtcgaa agaaccgcat aagagagaac aagaacagat tccaagagcc
     4681 aatgacggag tgatcgatgc tcaaagttat atgaatcgga tgatcttaag aaagggataa
     4741 accagatgga gagatatgac gacggattcg cagaagaaag acaacggatc gaacgattag
     4801 tagaggagat cattgctgga gaacataaga ttgataaact gccgaattct tccgcaggat
     4861 ttagtggagc tgcacaggca gaatattttt cgaaggcaag atatcattta gaatcggatg
     4921 taagtaatct gatcgtgaat cataaacagc aactggatga attacaattg ttaaagatga
     4981 taaaggggca agaatgatag aacacaattt atttgagatg gaaaattaca gagagctgat
     5041 cgatgaggtc ggtgtcgata aattccgtga acggtttgag gaactgcaga agacagcgat
     5101 ggaatttatt gaaatggcag gattttctga gacatcttac tgtaatgaaa gaattttaat
     5161 gcaggtgatc cttgattatt ttatggatgt gatgcggtta aaggaatttc atagtattga
     5221 gaggatccgc acagaaaagc tgtttgcata cacgatttcc tggatcgttc ggagaaaacc
     5281 aattcagttt cgggattatt ccgaagaaga gagagatatt tttatcaatg aacgttttgc
     5341 tgcatatctg ctcatcaatg aatgtctgat gtgtggaacg aaacattttg tacaggaagc
     5401 ttatgcagaa aaattagtag aatatacaga aatgctgcta tattatttca aataccgtca
     5461 gtgcgatcca aagacattag aacttttgat cgaatcattt aagatgggcg gacttgtgca
     5521 ttagaaggaa aggggattta ggtatgggaa tccttgtttt tgatgtagga acctcgagta
     5581 tgaaaggtgc attgctaaac gataaggcag agattttatg ccagtttcac agaaaatatc
     5641 atccaacctt ttattcttca gtcaaggtaa cgcaggaccc agaaatatgg agaaaagcat
     5701 tatatgatat agcaagagat gtgggtgact ggtgtaagga tcagaacgaa gaagtagaga
     5761 tgatttcttt gacggcacaa agaacatcga tcataccagt tgatcataaa ggagatccat
     5821 tatgtgatgc tgtgatgtgg caggataaaa ggcatattga agtttgtacg aggctttctg
     5881 cgatgaatgg acagatcatc agaaaaagcg gtacaatggt gaatcctgta ttttcaggga
     5941 gtaagatggc atggcttaaa gagacacaac cggagattta taagaaagca gacaggattt
     6001 ttgttgttgc agattattta ttatatcata tgacagggca gagaaaactt gatcgtactt
     6061 atgctagccg gtctcatttg atgaatttaa gaaccgggcg gtgggacagc gtgcttcttg
     6121 atatctttgg aatcgagaag aataagcttt gtgatctgat cgatccagga gaacttcatg
     6181 gatatacaaa tgagaaattt gcggcaagta caagacttaa gacgggaatt ccagtctatt
     6241 cggctggtgg tgatcagcag tgcagcgcgc ttgggatggg aatttatgaa gaaggtgaca
     6301 tggaggttac aagtggaacg ggcgcattta tcatagggat cgcagagaaa gtaccaattg
     6361 tgcttctggg taatccgatc attaattatt ctgcggtaaa aggaaaatat attgtggaag
     6421 caagtatctt aacatgtagt gcggcatttg actggttttg caataatttt tataaagaag
     6481 tgaatggaga tgtttatcaa aagatcgatc aggaaatctc acagtctcca ccaggagcca
     6541 atggatgtat gttgcttcca ttttttcagg gaagggcaac accacagtgg aatccaaaag
     6601 caactgcatc atttacaaat gtaacgctga gtacgacaag aggcgatatg gtgagagctt
     6661 tgttggaagg aattgcttat gagatccgca acaatttaga cattgtggaa ggtcagattg
     6721 gcaaggcaga tcagattcat attggtggtg gcttgagcaa cagccatgaa tttaatcaga
     6781 ttcaggcaga tgtctatgga agaaaactga tccattatca gcatagggaa gcagggatca
     6841 ttggagcatg gacaagcaca atggtggaaa gtgggaaagt ttcagatcat gaggaggctc
     6901 tagaacgggc aggtaggaat aattcattac agatttatga acctgttgaa aaaaatattg
     6961 agaaatatca ggaacttcga gcagatatga accatttata ccaaaaaata tattaggaat
     7021 aaacaagaat atttataaaa aactgaacca ctgtaaaagg ggcaactcat aggagtaatt
     7081 tatgagttgt ctcttttttt gtacatttta aacaaaatgt atgggtttgc acttgtgtgc
     7141 atatggattg tatttatgga agaacaatat atttataaat gtcaatctaa aatatttatt
     7201 ttgcacatat gtgcaaagat tgagaatttg ttgcacggat gtgcagcgaa tgataagatg
     7261 taaacaactt aaggagatgc acataaaagc aacatagaaa atatggagaa tgaattagga
     7321 ggaaccaaat gagtgaagta atcgaagcaa gaaacattaa gttgaatgta gacgcatctg
     7381 attggagaga ttccatggtc aaatcaggac agctcttagt agatagcgat tatattacaa
     7441 aagattacat tgatttgacg atcaaatgtg tggaagaaaa cggaccatat attgttatca
     7501 ttccaggact tgcattatct cattcaagac cagatgtgag tgtaaataaa acaggattaa
     7561 gtttgattac attaagcaag ccagtctgtt ttgactgtga taatgatcca gtagacattg
     7621 ttcttactct ggcagcaact gacgacacat ctcatctgca gaaacttcag tccatggctg
     7681 agtttatttc agatgaagag aacattgaat tcattaaaaa cgcaaaatca gctgaggaag
     7741 ttgccaaagc gatcaatgaa tttgaaccag aagagtaaga agggagaaag aggatggaaa
     7801 tattaaacac agtatttaca tttgtatcga acaatatttt gacgaaagcg gagttcttcg
     7861 taggactgat cgtcttagtt ggatacatct tattaggaag aaaatggtat gaaacattag
     7921 caggtttctt aaaagcagtt gtaggttata tgattttaaa tgtaggttca gctggattga
     7981 tcaacacatt ccgaccaatc cttgcaggat taaaccagaa gtttcattta aatgcggtag
     8041 ttattgaccc gtattttgga ttgaatgcag taaatgcagc acttgaaaat atcggattat
     8101 ccgcagcatg gactatgaca tcattgttac ttgcatttat ctggaacatc atcttattga
     8161 tcttccgtaa attcacaaaa cttcgtacct tattgttaac tggacatatc atggtacagc
     8221 aggcaacaac cgtaacatgg atcgtattct tatttatccc aggacttcgt aatatctggg
     8281 gagctgtagc agtcggaatc ttagtcggta cataccaggc agtattttct aacttaacag
     8341 ttgaaccaac agatcgttta acagacggtg aaggtggatt tgcgatcgga catcagcaga
     8401 tgtttgcaat ctggttagca gacaagatcg caccaaagat cggtaaaaaa gaagattcta
     8461 ttgaaaatat caaacttcca ggattcttac agatgtttag tgacaatgta gtatctacat
     8521 ccgtattaat gtttatattc tttggaatta tcatgttagt acttggggaa gatttcatga
     8581 gaagtttaga tgacacattc tcacagacaa cagcatttgg attctatatt ttctctaaag
     8641 cattaagctt tactgtttac ttaaacgtat tacaggcagg tgttcgtatg ttcgttgcag
     8701 aacttacaga atcattcaaa ggtatttcag acaaactgct accaggagca atcccagcag
     8761 tagactgtgc ggtagcttat ggattcgcac cagcaaacgc agtaacagta ggtttcttct
     8821 gtggagcact tggacagttc ttagcgatcg caggactttt gatcttccat tcaccagtta
     8881 tgatcatcgc aggattcgta ccattattct ttgataacgc aagtatcgca gtatatgcaa
     8941 accacagagg tggagttaaa gcagccatga tcttaccgtt tatcagtgga atcattcagg
     9001 tattaggagg agctgtttgt gcttacgttt tacagttagt agcattcggt ggatggcatg
     9061 gaaacttcga cttctcaacg atcttccttg cagtatctta tattgttaag tatttaaagg
     9121 tacctggagt tatcctttgt gttatcgcaa tgttagttat tcctcagatt cagtatgcga
     9181 aatctgataa agaaaagtat ttcacagtag gtcaggaaga cgacgagtac taaaaagcaa
     9241 tatcaaatca atttttaata aaacaatcaa tcagccaaaa ttcaggaggt atttattatg
     9301 aaaatcaaag caatcgcagc atgtggaaat ggaatgggat caagtcagat gatcaaaatg
     9361 aagatcaaca aagtattcaa aagattagga attgatgcag aaatcggaca tatgagtgtt
     9421 ggagaagcaa aaggtgtagc aaacaattac gatatgatct ttgtatctga aacatttaca
     9481 tcgcagttta gaacaggaaa agatacaaaa gtcattggac ttaaaaattt attatcagaa
     9541 aaagaaatcg aagcaaagat cagagaagca ttagatatgc ctgcagaata aatcggggtg
     9601 agatagatgt acgatatcaa aaaaaggcca ttagggatat atgaaaaagc tcttccaaat
     9661 gatttcacat ggcttgaaaa aatgcaggca gcaaagcagg caggatttga ttatattgag
     9721 atttctattg atgaatctga tgagagatta gcacgacttg attggagtga tgaacagatc
     9781 gaagaaatcc gctcttatat gaaagagaca gggatcatca ttccaagcat gtgcttaagt
     9841 ggtcatagaa gattcccatt tggaagcaag aacaaagaaa taagagataa agcatttgag
     9901 atcatggaaa aaggaatcat cttagcacag aagttaggag tcagagtaat ccagcttgca
     9961 acttatgatg tatattatga agaatctgat gaagaaacaa aagctttatt cttagaagga
    10021 atgaaaaaat cagtcgagat ggcaagccac gcaggagtga tccttgcaat ggaaatcatg
    10081 gatacgccat ttgttggaac gattcttcgt gcaatgcact atatcaaaga aatccgttcc
    10141 ccatatttta agatttatcc agatatggga aatctgacgc agttttcagc ggatgttgag
    10201 agtgaatttg aacttggaat cagtgaaacg gtagcgatcc atgtcaaaga gacaaaccct
    10261 ggagtattta aatgtgttcc atttggagaa ggaaccgtaa gatttacaga catttttaaa
    10321 aaattaaaag atttaaatta cacaggaatg ttcttgatcg aaatgtgggc agataacaca
    10381 aagaaacaaa cgatggaaga agcagttgaa acaatcaggc aggcacgctt atttgtagaa
    10441 gataagatgg aacaggcagg gatcaaagta cctgcataag gaggaaatca tgtatcgaat
    10501 ggtagtgacc gatcttgatg gaacattatt aaacaataaa aaacaggttt cagaggccaa
    10561 tgtaaaggca attcaaaacc taaaagatca aaatataacc tttgttatgg caacaggacg
    10621 atcagatgtt atgacgagag catacacaaa acaattaaaa aatgtcgata ttgtcattgg
    10681 gtgtgatgga gctgtcattc gaaacattag gaccggagaa atcttatgtg aaaatcattt
    10741 aagaagtgaa acctgtcata cgacatttga aatttgtaaa aaatatggat tacagtatta
    10801 tgtctttgca aaagatgaac tagtatccga tgatcctcag aatgagagat ttctggtcca
    10861 tcagaaattc aatcagactg tagaggaaga tgaagagata ccaatgcaga ttgtggatga
    10921 tttaaatgaa tatgtaaatg atcatatcat ttacaagatc gttgtaagtc acaatgacac
    10981 aagttattta gataaagtag ccgaagtgat caaaaaagaa acggatgcag atgccattcg
    11041 ttctgggaaa aaagtccttg cagttaaagc aaggggagtt tccaaagcag aagcgatcaa
    11101 aaagcttgca caaaagttag gtattttgat caaagatatc atcgcatttg gtgatgaagt
    11161 aaatgatatt gagatgctta cgttggttgg acttggaatc gcaatggaaa atgcagatga
    11221 tgtcgtgaaa gaggcagcag accagattgc aggaagaaat gatcaggacg gcgtaggaaa
    11281 agaattagag aaaatatttg gaaatgcagg aaagtaagaa aggagttaag aagatgcttg
    11341 atgaattaaa acagcaagta tacgaagcaa atatggaatt accggcaaaa ggactggtaa
    11401 cttatacatg gggaaatgtc agtgggattg acagagaacg tggtttgttt gtcattaagc
    11461 caagtggtgt tgactatgaa aaactaacac cagatatgtt agttgtcatg gatttgaatg
    11521 gggaaaaagt tgagggagat ttaaatcctt cctctgatac accaactcat atcgagcttt
    11581 acaaagcatt tccagaagtc ggtggaatcg ttcatacaca ttctccttgg gcgacttcat
    11641 gggcacaggc aggaagaagc attccatgtt atggaacaac acatgccgat tatttcttcg
    11701 gagatattcc atgtgcacga tctttaacag ccgatgagat caacggagag tatgaaaaga
    11761 atacagggat tgtgatcgca gaaacatttg aaggaaaaaa tccaatctat gtaccaggtg
    11821 tcttatgtac aaaccatggc ccattttcat ggggagcaga cgcagcagaa gcagttcata
    11881 atgcagttgt tatggaagaa gtagcaaaga tggcgtaccg ctgtgaacat ctgaaaaaag
    11941 atattgagga agcaccacaa gtattacagg acaaacattt cttcagaaaa catggagaaa
    12001 atgcttatta cggaaatgac ttataggata tagagataga agatttcgga tagaaaagga
    12061 gatcaaggtt atgagtaaaa ttagtgaaat gacaagagaa agctggattg aaagcacatt
    12121 cccagaatgg ggaacatggt tagtagaaga tattgaaaat gaagtagtag caccaggaaa
    12181 tgtggcaatg tggt
//
LOCUS       DAOMUE010000027        11983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1241_length_11983_cov_2.576040, whole genome shotgun sequence.
ACCESSION   DAOMUE010000027 DAOMUE010000000
VERSION     DAOMUE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 11983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11983
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1241_length_11983_cov_2.576040"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLC71_02520"
     CDS             <1..560
                     /locus_tag="PLC71_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HRM01553.1"
                     /translation="IVVRETELEKTLEEIGKKPALVITDSQAFGKVSKIVPEDIKLTS
                     FSILFARYKGDLEEEVRGVKALEHLEEGAKILIAEGCTHHRQCDDIGTVKIPRWLKQY
                     TGKDFDIHTSSGNSFPEDLDTYDLVIHCGGCTLNRREMKNRIARVKDAGVPIVNYGIF
                     IAATQGILKRTLEPFEEASRILNEI"
     gene            680..2140
                     /gene="gltX"
                     /locus_tag="PLC71_02525"
     CDS             680..2140
                     /gene="gltX"
                     /locus_tag="PLC71_02525"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRM01554.1"
                     /translation="MSKVRTRFAPSPTGRMHVGNLRTALYAYLIARHAGGDFILRIED
                     TDQERQVEGAEEIIYNTLKKAGMNHDEGPDIDGGVGPYVQCERQAQGLYLKYAKELID
                     KGEAYYCFCDQERLDTLKVKSGDVVISHYDKHCLNLSKEEVQAKLDAGVPYVIRQNNP
                     TEGTTSFVDEIYGEITVDNIELDDMILIKSDGYPTYNFANVVDDHLMGITHVVRGNEY
                     LSSTPKYNRLYDAFGWEKPVYIHCPLITDEEHHKLSKRKGHSSFEDLIEQGFLPETIV
                     NFVALLGWSPGGEQEIFSLKELEEIFDYKHMSKTPAVFDMNKIKWMNGEYIKAMDFDR
                     FKELAMPYVTETIHREMDFDKILSMVKTRIELFTEIPGHIDFFEAVPEYDVEMYKHKK
                     MKTTPETSLTVLKEIYPVIEAQEDFTNDALYEMLVSFAKGHEYKNGYVMWPVRTAVSG
                     KQMTPGGATELMELLGKEESLKRINDAIAKLEAALA"
     gene            2172..>3333
                     /locus_tag="PLC71_02530"
     CDS             2172..>3333
                     /locus_tag="PLC71_02530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRM01555.1"
                     /translation="MHKDGPMMVLAGPGSGKTLVITKRIQYLISHYQIPPQRILVITF
                     TRAAANEMKERFWRLAGETLPVSFGTFHSVFFTILKYAYHYSADNILPEHKKYDFIRE
                     IITDHELEIDDEADFMRQIIQEISLVKGQMIPLDYYHSGCCGDEVFKEIYRAYEEKLH
                     ENRWIDFDDILVYTYELLKEREDIRKAWQQKFPYILIDEFQDINKIQYEIVKMLAGET
                     KNLFIVGDDDQSIYKFRGARPELMLNFPKDFENTRQVILNRNYRCGEEIVQVAEDIIS
                     YNTKRFEKKMQAREDAASMVEVRTFKDHYEENKHIIYTIKEEMAKKTPLSQIAILYRT
                     NQGPRQLIAALMAYNIPFYMQDAVPNLFDHWISKNIIDYMHLAMGDRKRSTFL"
     assembly_gap    3334..3343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3344..4002
                     /locus_tag="PLC71_02535"
     CDS             <3344..4002
                     /locus_tag="PLC71_02535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRM01556.1"
                     /translation="MNRPKRYISREYLTESQVSFDDLLEKVKDKPWLYEYVEDFKEDL
                     RIMKNMTPLMAINYIRKSVGYNAYLAEYARFRKIQEEEFTQVLEELQESAKGYDTYEA
                     WFDHIKEYTEELNRQSKENQKEKEGVVVSTMHSTKGLEFERVFLPDVNEDVIPHKKSM
                     KEEDVEEERRLFYVGVTRAKKYLHILSVKKLYNKDSRPSRFVEELRSRQEKRGVLHGK
                     "
     gene            3992..4570
                     /locus_tag="PLC71_02540"
     CDS             3992..4570
                     /locus_tag="PLC71_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HRM01557.1"
                     /translation="MENRAMPRAGEFYMHFKGKLYQIVTVAIHTETEEPLVVYQAMYG
                     TFKTYARPLAMFLSEVDHEKYPDVQQTYRFQKVELCENAGDQYGIKIKETEQDSEAEL
                     VDEVTAQENECDETEETADIEPLLKFLDETDLHERLNILLQYRDQITETMLESMGMAM
                     DCVLNGKTLEEKYYELDKVIRTKLQYEKKPRI"
     gene            complement(4600..4917)
                     /locus_tag="PLC71_02545"
     CDS             complement(4600..4917)
                     /locus_tag="PLC71_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HRM01558.1"
                     /translation="MSEFNEVDNIIEEETPVLHLELEDGTEQDCAVIALFSVEELDDQ
                     EYIALITVEDLESDDEEGALLLYRYNEDPEDPDTFSLDNIETDEEYETVTAVLDEILE
                     DEE"
     gene            5062..6228
                     /locus_tag="PLC71_02550"
     CDS             5062..6228
                     /locus_tag="PLC71_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRM01559.1"
                     /translation="MEEYKFLLDIAIILAMTKSFSLVSKKFNMPPVVGALIAGIILGP
                     VVLNVVSPSDSVLNLAEVGVVVLMFQAGLETDIDELKKSGIPAFVIALCGVLIPLIMG
                     AVLSVIYGNNIMESIFIGVILTATSVSITVEALQDMGHLKGRVGTAILGAAIIDDILG
                     IVLLSIMTSLGGASGFHIQAILKILIQMAAFFAISIIGGVGIKKFFEWLSTYKNPEIE
                     RHRRRVPVFALAFCLALAFLAEVFGIADITGAYLAGIILCRLPERFYIYNKIEVLSYM
                     LITPVFFASIGLNVNIHGMTQTLIIFTIILCVIAVISKIAGCGFGALLCKYTKRESVQ
                     IGAGMVCRGEVALIVAQKGISVGLLSEIFFAPVVIMVLVTTLLSPIILKMLFAK"
     gene            6292..7230
                     /locus_tag="PLC71_02555"
     CDS             6292..7230
                     /locus_tag="PLC71_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRM01560.1"
                     /translation="MIDTVVSVKLPIGEMLRIKRNRLMPDVVTGEEKRICLVSGIYGD
                     ELGGQYICGEIIRRIKAEFENLTGIVDVYPAVNPLGLDARTRAVPISDIDYSSAFPGD
                     INGGLEDYTAAKLIEDIKGASCCIDIHSSNIFLQEVPQVRLNNDYDETLLHLSKYMNT
                     DLVWIHPSQTVNEGSLAFTLNQMGIPTMVTESGAAFRIRYEYCRQIIDGIFSVMAELG
                     IWKGKVQGKTKVPFIHEHQIFYLNCESSGLFVSYKRLKDPVKKGEIIGIVQNPLMGAT
                     EEEVVAPTDGILMTLREHPSVTEGSLVARVIGGNAL"
     gene            7227..8174
                     /locus_tag="PLC71_02560"
     CDS             7227..8174
                     /locus_tag="PLC71_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HRM01561.1"
                     /translation="MKKEIIYSSGNYLRDEFKIEGYLFGKQSEGSEPAACIVGAMRGN
                     EYQQLYICSQLVKRLKELENSGAIVGDNQILVIPCVNNFSMNVGMKYWVSDNSDINRQ
                     FPGNFDGEPTSRLAAHLFERVKGFRYGIHFASFFRSGDFVPHVRMPATGKESTSLASL
                     FGLPYVLTSKQRNFDKKTLTYNWQINGTDAFSVYSGETDNIDVNLARKAVSAVLRFLT
                     RMGILKYNCHNGYIASIIEEEDLVSVKASAPGFLRRFVTINEEVNRGQLLGEVINPYN
                     GEILSEIRSTADGIIFYAEDQPTILENASAFQVIKKLHE"
     gene            8188..11943
                     /locus_tag="PLC71_02565"
     CDS             8188..11943
                     /locus_tag="PLC71_02565"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRM01562.1"
                     /translation="MGEVRRVYVEKKKDYAVKAKELHASLKQYLGLDVESVRVLVRYD
                     IENLSDASYEKALVTVFSEPPVDDVYEETLPLADGEISFSVEFLPGQFDQRADSAQQC
                     VKLLNEDEDALIRSATTYVVKGDLTDEQLEAVKEYCINPVDSREAAADKPETLVMDFE
                     EPADVKILDGFKDMEEDKFKELYDSLNLAMTFKDFLHIQNYYKGEEKRDPSMTEIRVL
                     DTYWSDHCRHTTFSTELTSIGFQDGFYRAPLEEAYYSYMDDFEEMYKDRDDKYVCLMD
                     MALMAMKRLKKDGILDNMEESDEINACSIVVPVEIDGKEEEWLISFKNETHNHPTEIE
                     PFGGAATCLGGAIRDPLSGRSYVYQAMRVTGAADPTVPLKDTLKGKLPQYKIVNEAAN
                     GYSSYGNQIGLATGYVNEVYHPDYVAKRMEIGAVMGAAPRKNVIRENSDPGDIIILLG
                     GRTGRDGCGGATGSSKVHTEESIETCGAEVQKGNPPTERKIQRLFRREEVSRLIKKCN
                     DFGAGGVSVAIGELADGLVVDLDKVPKKYAGLDGTELAISESQERMAVVVDPKDVDQF
                     LAYSAEENLEAVEVAVVTEDPRLVLKWRGKEIVNISRAFLDTNGAHQETEVFVEIPHR
                     VESYFNRKDETGTFEEVCKNTLSDLNVCSQKGLVERFDSSIGAATVTMPYGGKYQMTP
                     IQTMVAKLPVLKGECDTVTMMSYGFDPYLSKWSPFHGAVYAITESIAKIVASGGDYRN
                     IRLTFQEYFKRLGEDKTRWGQPVAALLGAYQAQMGFGLASIGGKDSMSGTFNDIDVPP
                     TLVSFAVDVAKTTDVITPELKRAGNRLIRICIPTDAYNLPIYSEVKKVYKRITKLIKD
                     GIIKSAYAIGGGGALEAVAKMAFGNKLGVIFDEEVELKDLTDPKFGSFVVEMSTRDVH
                     KLAIPGLMLGEVTDKHEFVFGDEVVTLEEAIDTWKKPLEKVFPTRSEGDQSIVATTRH
                     YTKGNIYVCKNKVAKPNVFIPVFPGTNCEYDSAKAFERAGANVITKVFKNLDAQGIRD
                     SVEVFEKAIKESQIIMFPGGFSAGDEPDGSAKFFATAFRNEKLKEAVMDLLNNRDGLA
                     LGICNGFQALIKLGLVPEGKIVEQNAKSPTLTTNRIGRHISKVAYTRIASNLSPWFNN
                     VHVDDIFSIPVSHGEGRFVADEDVIQKLFENGQVATQYVDLNGDPTMNEEFNPNGSYH
                     AIEGITSPDGRVLGKMGHSERRDSYVGINIYGQKDQKIFESGVEYFK"
BASE COUNT         4047 a         1990 c         2760 g         3176 t
ORIGIN      
        1 ctattgttgt aagagaaacg gaattagaaa agacattaga agagatcggc aagaaaccag
       61 cattggtcat tacagactct caggcatttg gcaaagtctc aaagattgtg ccagaagata
      121 tcaaactgac ttcattttcg attctgtttg caagatataa aggtgatctg gaagaagaag
      181 tcagaggagt gaaagccttg gaacatttag aagaaggggc gaagatcttg atcgcggaag
      241 gatgcactca tcacagacag tgcgatgaca tcggaacggt gaagatccca agatggttaa
      301 aacaatatac aggtaaggat tttgatatcc atacaagcag tggaaactct ttccctgaag
      361 atctggatac ttatgatctg gtcattcatt gtggcggatg tactttaaat agaagagaga
      421 tgaaaaaccg tatcgcaaga gtcaaagatg caggtgttcc gatcgtgaac tatggaatct
      481 ttatcgcggc aacgcaggga atcttaaaga gaaccttaga gccatttgag gaagctagca
      541 ggatcttaaa tgagatttaa gacggattgt ttcaaaaaat caacataaat taacgaaaat
      601 caataagact gcttctttac aaagaaagat aagttttgta gaatagaaag atagaattaa
      661 aaccaaatgg gaggaaatca tgagcaaagt aagaacaaga tttgcaccaa gccctacagg
      721 gcgtatgcat gtaggtaact taagaacagc cctttatgca tatctgatcg cacgtcatgc
      781 aggcggagat tttattttaa gaattgaaga tacagaccag gaaagacagg tagaaggggc
      841 agaagagatt atctacaata cccttaagaa agccggaatg aaccacgatg aaggaccaga
      901 catcgatggt ggagtcggac catatgttca gtgtgagaga caggcacagg gtctttattt
      961 aaaatatgca aaagaactga tcgacaaggg agaagcatat tactgtttct gtgatcagga
     1021 gagattagac acactgaaag taaaatcagg agatgttgtg atcagtcatt atgacaaaca
     1081 ttgcttaaac ttatccaaag aagaagttca ggcgaaatta gacgcaggag taccatatgt
     1141 tatccgtcag aataacccaa cagaaggaac aacatctttc gttgatgaga tttacggaga
     1201 gatcacagtg gataatatcg aattagatga tatgatcctg atcaaatcgg atggatatcc
     1261 aacatacaac ttcgcaaacg tagttgatga ccatttaatg ggaatcacac atgtagttcg
     1321 tggtaatgaa tacttatcat ctacaccaaa gtacaatcgt ttatatgatg cattcggatg
     1381 ggaaaaacca gtatacattc attgtccact gatcacagac gaagaacatc acaaattgag
     1441 taagagaaaa ggtcattctt ccttcgaaga tctgatcgag cagggattct tgccagagac
     1501 gatcgtaaac ttcgttgcat tacttggatg gagtccagga ggagaacagg aaatcttctc
     1561 attaaaagaa ttagaagaga tctttgatta caaacatatg tctaagacac cagcggtctt
     1621 tgatatgaac aagatcaaat ggatgaatgg tgaatatatc aaagccatgg actttgaccg
     1681 tttcaaagaa cttgctatgc catatgtaac agagacaatt cacagagaga tggattttga
     1741 taagattctt tctatggtga agacaagaat tgagttattt acagagattc cgggacacat
     1801 tgacttcttc gaagcagttc cagaatatga tgtagagatg tacaaacata agaagatgaa
     1861 gacaacacct gagacttcat taaccgtatt aaaagagatc tatccagtga tcgaagcaca
     1921 ggaagatttt acaaatgatg cattatatga gatgttagta tcttttgcaa aaggacatga
     1981 gtacaagaat ggttatgtaa tgtggccagt tcgtacagca gtctctggaa agcagatgac
     2041 accaggtgga gcaacagaat taatggaact tctgggaaaa gaagaatcat taaagagaat
     2101 caatgatgcg atcgcgaaac tggaggcagc acttgcatag ccagctgagc aaggcacaga
     2161 aagaagcagt gatgcacaaa gatggaccga tgatggtact tgcaggacca ggttcaggga
     2221 agacacttgt gatcacaaag agaattcaat atttgatatc gcactatcag atcccaccgc
     2281 agcggattct ggtcattaca tttaccagag cagcagcgaa tgagatgaaa gagcggtttt
     2341 ggcggctggc aggggaaacc ctgccggttt cttttggaac ctttcattct gtgtttttta
     2401 cgattttaaa atatgcctat cactatagtg cagataatat tctgccggaa cataaaaaat
     2461 atgactttat ccgtgagatc atcacagatc atgagctgga gattgatgat gaagcagatt
     2521 ttatgcgtca gatcatacaa gagatcagct tagtcaaagg gcagatgatc cctcttgact
     2581 attatcacag tggatgctgt ggggatgaag tgtttaaaga aatttaccgg gcatatgaag
     2641 aaaaactgca tgagaaccgt tggatcgatt ttgatgatat tttggtttat acgtatgagt
     2701 tgttaaaaga gcgtgaggat atcaggaaag catggcagca gaagtttcca tatattttga
     2761 tcgatgagtt tcaggatatc aataagattc agtatgagat cgtcaagatg ttagcaggag
     2821 agacaaagaa cctctttatc gtaggagatg atgatcagtc tatttataag tttcgaggtg
     2881 caagaccaga actgatgtta aattttccaa aggattttga gaataccaga caggtgatct
     2941 taaaccgaaa ctatcgttgt ggggaagaga tcgtacaggt tgcggaagat atcatttctt
     3001 ataatacaaa acgttttgaa aagaagatgc aggcaagaga agatgctgca agtatggttg
     3061 aggtccgtac atttaaagat cactatgagg agaataagca tatcatttac acgatcaagg
     3121 aagagatggc aaagaagaca ccgttatccc agatcgcgat cctttatcgg accaatcagg
     3181 gaccaagaca gctgattgct gcattgatgg catacaatat cccattttat atgcaggatg
     3241 cagtgccgaa tctgtttgat cactggattt ccaaaaatat catagattat atgcaccttg
     3301 caatgggaga tcgcaaaaga agcacatttt taannnnnnn nnntcatgaa ccgccccaag
     3361 cgttatatca gcagggaata tctgaccgaa tcacaagtgt catttgatga tctgttagag
     3421 aaggtcaaag ataaaccatg gctttatgaa tatgtagaag attttaagga agatcttcgg
     3481 atcatgaaga atatgactcc gttgatggcg atcaattata tccggaaatc cgtgggatac
     3541 aatgcgtatt tagcagaata tgcaagattt cgcaagattc aggaagaaga atttacgcaa
     3601 gtattagaag aacttcagga gagcgcaaaa ggctatgata catatgaggc atggtttgat
     3661 catatcaaag aatacaccga agaattaaac cgccagtcaa aagagaatca gaaagaaaaa
     3721 gaaggagttg tggtcagcac gatgcacagc accaaaggat tggaatttga acgtgtcttt
     3781 ttgccagatg tcaatgaaga cgtgatccca cataagaagt ccatgaaaga agaagacgtg
     3841 gaagaagaaa gacgtttgtt ttacgttgga gttacaagag ccaagaaata tctgcacatc
     3901 ttgtcagtaa agaaacttta taacaaagac agcaggccgt caaggttcgt agaagaacta
     3961 agaagccgtc aggagaaaag aggtgttttg catggaaaat agagcgatgc caagagcagg
     4021 cgaattttat atgcatttta aaggaaaact ttatcagatc gttacggttg caatacatac
     4081 agagacagaa gaaccattgg tggtctatca ggcaatgtat ggaacgttta agacatatgc
     4141 aagaccactt gcgatgtttt taagtgaagt tgatcatgaa aaatacccag atgtgcaaca
     4201 gacatatcgt tttcagaaag tagaattgtg tgaaaatgca ggagaccagt atggtataaa
     4261 aatcaaggaa acagagcaag attcagaagc agaactggtt gatgaagtga cagcacagga
     4321 aaatgaatgt gatgagacag aagaaacagc agacattgaa ccactgttga aattcttaga
     4381 tgaaacagat cttcatgagc gcctgaatat tttattacag tatagagatc agatcacaga
     4441 aacgatgctg gaatccatgg gtatggcaat ggattgtgtc ctgaatggaa aaactctgga
     4501 agaaaagtat tatgaacttg acaaagtgat ccgaacaaaa cttcaatatg aaaaaaagcc
     4561 ccgcatataa gatgcaaggg cttttttatt aatatcatat tattcttcgt cttctaagat
     4621 ctcatctaag acagcagtga ctgtctcata ttcttcgtct gtttcaatgt tgtcaagact
     4681 gaaagtatca ggatcttcag ggtcttcgtt gtaacgatat aaaagtaaag ccccttcttc
     4741 gtcatcactt tctaagtctt caacagtgat caatgcgata tattcctgat catctaattc
     4801 ttctacagag aataaagcga tcacggcaca gtcttgttca gttccatctt ctaattctaa
     4861 atggagcaca ggagtttctt cttcaatgat attatctact tcgttaaatt cactcatttt
     4921 gatacctctt ttcttagtct tttctccact atactaaagg atcataaaaa aagcaagaaa
     4981 aggataaaaa aagatcactt tgtctaagaa ttgacaagaa aaaacaggta tgatacgata
     5041 cgaactaatg gaggtaaaac aatggaagaa tataagtttt tgctggatat cgcaatcatt
     5101 ctggcaatga ccaagtcttt tagtcttgtg tcgaagaagt ttaacatgcc gccagtcgtt
     5161 ggagcattga tcgcgggaat cattttagga ccagtggtcc ttaatgtagt gagtccttca
     5221 gattcagttt taaatctggc tgaagtaggt gtagttgttt taatgtttca ggcaggtctt
     5281 gagacagata ttgatgagtt gaagaagagt ggaattcctg cattcgtcat tgcgttatgt
     5341 ggagtattga tcccacttat catgggagct gttttatccg ttatatatgg aaataatatc
     5401 atggaatcga tctttatcgg agtaatccta acagcaactt cagtaagtat cacagtagaa
     5461 gcattacagg atatgggaca tttaaaagga agagtcggaa cagcaatcct tggggcagcg
     5521 atcattgatg atatcttggg aatcgtttta ttatcaatta tgacaagcct tgggggagca
     5581 tctggattcc atattcaggc aatccttaag atcttgatcc agatggcagc attctttgcg
     5641 atttctatta tcggtggagt tgggatcaag aagttctttg agtggctttc tacttataag
     5701 aatccagaga ttgaacgtca tagaagaaga gttccggtgt ttgcacttgc attttgctta
     5761 gcacttgcat ttcttgcaga agtgtttgga atcgctgata tcacaggtgc atatcttgca
     5821 gggatcattt tatgcaggct cccagaacga ttttatatat ataacaagat tgaagtttta
     5881 tcgtatatgc tgatcacacc agtattcttt gcaagtatcg gtctgaatgt aaacattcat
     5941 ggaatgacac agacgttgat catctttacg atcattttat gtgtgatcgc agtgatctcg
     6001 aagatcgcgg gatgcggttt tggagcattg ttgtgcaaat atacgaagag agaatctgta
     6061 cagatcggag caggaatggt atgccgaggt gaagttgcac tgatcgttgc acagaaagga
     6121 atttctgttg gattattatc agaaattttc tttgcaccag tagtcatcat ggttctcgtc
     6181 acaacactct tatcaccgat cattttaaaa atgttatttg caaaataaga cgtgagggtc
     6241 aaaagtaaaa tatttacgat acagataaaa caatagaaaa gaggctttaa tatgatagac
     6301 acagtcgtat ctgtaaaact tccgatcgga gagatgctaa ggatcaaaag aaatcgtctg
     6361 atgccggatg tggtgactgg agaggagaag agaatctgtc tggtgtctgg aatctacgga
     6421 gatgagcttg gcggacagta tatctgcgga gagatcattc gccggatcaa ggcagaattt
     6481 gagaatctga caggtattgt cgatgtgtat ccagcggtca atccattagg actggatgca
     6541 cgaacaagag cagtaccaat ctcagatatc gattacagca gtgcatttcc aggagacatc
     6601 aatggaggtc tggaagatta tacagcagct aaattgatcg aagatatcaa aggagcatcc
     6661 tgttgcattg atattcattc tagtaatatc tttttgcagg aagttcctca agtacgttta
     6721 aataacgatt acgatgagac attacttcat ctgtcaaaat atatgaatac ggatcttgtc
     6781 tggatccatc cttctcagac agtgaatgaa ggaagtttag ctttcacttt aaaccagatg
     6841 ggaatcccaa ctatggttac ggaatccggt gcagcatttc gtatccgtta cgagtactgc
     6901 cgccagatta tagatggaat tttttcggtg atggcagaac ttggtatctg gaagggaaag
     6961 gttcaaggca aaacaaaggt tccgtttatc cacgaacacc agatctttta tttaaattgt
     7021 gaatccagtg gattgtttgt ttcttacaag cgattaaaag atccggtaaa gaaaggcgag
     7081 atcataggga tcgtacagaa tcctttaatg ggagcaacgg aagaagaagt ggttgcccca
     7141 accgatggta ttttgatgac attaagagaa catccaagtg taacggaagg gtccttggta
     7201 gccagagtga tcggagggaa tgcactatga aaaaagaaat catatattcc tcaggaaatt
     7261 acttaagaga tgagtttaag atcgaaggat atctctttgg aaaacaaagt gaaggatctg
     7321 aaccagcggc atgtattgtc ggagcaatgc gtggaaatga atatcagcag ttatatattt
     7381 gttctcagtt agtcaaacgt ttaaaagaac tggagaacag tggtgcgatc gttggagata
     7441 atcagattct ggtcattcca tgtgtgaata atttttcaat gaatgtcgga atgaaatact
     7501 gggtatctga taacagtgat atcaaccgtc agtttcctgg gaattttgac ggggaaccaa
     7561 caagccgtct ggcagcacat ttatttgaac gggttaaagg attccgctat ggaatccatt
     7621 ttgcatcatt tttcagaagc ggggattttg tgccacatgt gcgaatgcct gcaacaggaa
     7681 aagaaagcac cagccttgca agtctgttcg ggctgcctta tgtattaaca agcaaacaac
     7741 gaaattttga taaaaagaca ttaacttata actggcagat caatgggacg gatgcatttt
     7801 cggtatattc tggggaaaca gataatatcg atgtgaatct ggccaggaag gctgtatctg
     7861 ccgtgcttcg cttcttgact cgaatgggta ttttaaaata taattgccat aacgggtata
     7921 ttgcgagtat tatagaagaa gaggatcttg tatccgtcaa agcaagtgct ccgggattcc
     7981 taagacgttt tgtgacgatc aatgaggaag ttaaccgagg tcagctgctt ggtgaagtca
     8041 ttaatccata taatggagag attttatcag agatccgttc aacggcagat gggatcatat
     8101 tctatgccga ggatcagcca acgattttgg aaaatgcatc tgcatttcag gtgattaaga
     8161 aactacatga ataaatggga ggaaatcatg ggagaagtta gaagagtcta tgttgaaaag
     8221 aaaaaagact acgcagtaaa agcaaaagag ctgcatgcat cattgaagca gtatcttgga
     8281 ctggatgtcg aatctgttcg tgtccttgtc cgctatgaca tcgagaatct atccgatgca
     8341 agttatgaaa aagcactggt tacagtattt agtgaaccac cagttgacga cgtgtatgaa
     8401 gagacattac cacttgccga tggggaaata tctttcagcg ttgagtttct gccaggtcag
     8461 ttcgatcaga gagcagattc tgcacagcag tgtgtgaaac tgttgaatga ggacgaagat
     8521 gccctgatca gaagtgcaac aacttatgtt gtaaaaggtg acttaactga cgaacagtta
     8581 gaagcagtca aagaatattg catcaaccca gttgactcaa gagaggcagc tgcagacaaa
     8641 ccagagacat tagtgatgga ttttgaagaa ccagcagatg ttaagattct ggatggattc
     8701 aaagatatgg aagaagacaa atttaaagaa ttatatgatt ctttaaatct tgctatgaca
     8761 ttcaaagatt tccttcatat ccagaattat tataaaggag aagaaaagag agatccttcc
     8821 atgacagaga tccgtgtact ggatacttat tggtcagatc actgtcgtca tacaacattc
     8881 tccacagaac ttacaagcat tggattccag gatggattct atcgtgcacc attagaagaa
     8941 gcatattatt cttacatgga tgactttgaa gagatgtaca aagaccgtga tgacaaatat
     9001 gtctgcttaa tggatatggc attaatggcg atgaaacgtc ttaaaaaaga tggaattctt
     9061 gacaatatgg aagaaagcga tgagatcaac gcctgcagca ttgttgtacc agtagaaatt
     9121 gatggtaagg aagaagaatg gctgatcagt ttcaagaatg agacacataa ccatccaaca
     9181 gagatcgaac catttggtgg agcggcaaca tgtcttggag gagcgatccg tgatccatta
     9241 tcaggacgtt cttatgtata tcaggcaatg cgtgttacag gtgctgctga tccaacagtt
     9301 ccattaaaag acacattaaa aggtaaatta ccacagtata agattgtaaa tgaagctgcg
     9361 aatggataca gttcttacgg aaaccagatc ggtcttgcaa caggatatgt taatgaagta
     9421 tatcatccag actatgttgc aaaacgtatg gagatcggtg ccgttatggg tgcagcacct
     9481 agaaagaacg tgatcagaga gaactctgat ccaggagata tcatcatctt attaggtgga
     9541 agaacaggac gtgacggttg tggaggagca acaggatcat ctaaagttca tacagaagaa
     9601 tctatcgaga catgtggagc tgaagttcag aaaggtaatc caccaacaga acgtaagatc
     9661 cagagattat tcagaagaga agaagtcagc cgtctgatca agaaatgtaa cgactttggt
     9721 gcaggtggag tttccgtagc gatcggagaa cttgcagatg gtctggttgt agatcttgat
     9781 aaagtaccta agaaatatgc aggacttgat ggaacagaat tagcgatttc tgaatctcag
     9841 gaacgtatgg ctgttgttgt agatccaaaa gacgtagatc agttccttgc atactctgca
     9901 gaagagaact tagaggctgt agaagttgct gttgtaacag aagatccaag attagtactg
     9961 aaatggagag gaaaagagat cgttaatatc tcaagagctt tccttgatac aaacggagca
    10021 catcaggaaa cagaagtatt tgtagagatc cctcaccgtg tagaatctta ctttaaccgc
    10081 aaagacgaga caggtacatt tgaagaagtg tgcaagaata cattatctga tcttaatgta
    10141 tgttcacaga aaggtctcgt agagagattc gacagctcta tcggagcagc aacagttaca
    10201 atgccatatg gtggaaaata ccagatgaca ccaattcaga caatggtcgc aaaacttcca
    10261 gtattgaaag gagaatgcga tacagtcaca atgatgagtt atggatttga tccatatctg
    10321 tccaaatgga gcccattcca tggagcagtt tatgcgatca cagaatctat cgcgaagatc
    10381 gtggcttctg gtggagatta tagaaatatc cgcctgacat tccaggaata ctttaagcgt
    10441 cttggagaag ataagacacg ctggggacag ccggttgcag cactacttgg tgcatatcag
    10501 gcacagatgg gatttggact tgcatcgatc ggtggaaagg acagtatgtc tggaacattc
    10561 aatgacattg atgtaccacc aacactggta tcttttgcag tcgatgtagc gaagacaaca
    10621 gatgttatca caccagaact taagagagca ggaaaccgtc tgatccgtat ctgtatccca
    10681 acagatgcat acaatcttcc aatctattct gaagtgaaga aagtatacaa acgtattaca
    10741 aaactgatca aagatggaat tatcaaatct gcatatgcaa ttggcggcgg aggagcttta
    10801 gaagcagttg ccaagatggc atttggtaat aaattaggtg tgatctttga tgaagaagta
    10861 gaattaaaag acttaacaga tcctaagttt ggatcatttg ttgttgaaat gagcacaaga
    10921 gatgttcata aacttgcgat tccaggatta atgttaggtg aggttacaga caaacatgaa
    10981 tttgtatttg gtgacgaagt tgtaacatta gaagaagcaa tcgatacatg gaagaaacca
    11041 ttagagaaag tattcccaac aagatctgaa ggagatcaga gcatcgttgc tacaacaaga
    11101 cactatacaa aaggaaatat ttacgtatgc aagaataaag ttgcaaaacc aaatgtattt
    11161 attccagtat tcccaggaac aaactgtgaa tatgattcag cgaaagcatt tgaacgtgca
    11221 ggagcgaacg tgatcacaaa ggtatttaag aacttagatg cacagggaat ccgtgattca
    11281 gtggaagttt ttgaaaaagc gatcaaagaa tcacagatca tcatgttccc aggtggattc
    11341 agtgccggtg atgaaccaga tggatctgca aaattctttg caacagcatt tagaaatgaa
    11401 aaattaaaag aagcagtcat ggatctgtta aataaccgtg atggactggc acttggtatc
    11461 tgtaacggat tccaggccct gatcaaatta ggacttgttc cagaaggtaa gatcgtagaa
    11521 cagaatgcga aatcacctac actgacaaca aaccgcattg gtcgtcatat ttctaaggtt
    11581 gcttatacaa gaattgcttc taatctgtca ccatggttta acaatgtaca tgtagatgat
    11641 atcttcagta taccagtatc tcatggagaa ggacgattcg tagcagatga agatgtgatc
    11701 cagaaactgt ttgaaaatgg acaggtagca acacagtatg ttgacctcaa tggtgatcct
    11761 acaatgaatg aagaattcaa tccaaatggt tcttaccatg cgattgaagg tatcacaagt
    11821 ccagatggac gagttcttgg taagatggga cattcagaac gtcgtgacag ctatgtagga
    11881 atcaatattt acggacagaa agatcagaag atcttcgaat ccggagtaga atatttcaaa
    11941 taagagtaca aaaaaagaac cttgcaagaa aaagcaaggt tct
//
LOCUS       DAOMUE010000028        11827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1267_length_11827_cov_2.893646, whole genome shotgun sequence.
ACCESSION   DAOMUE010000028 DAOMUE010000000
VERSION     DAOMUE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11827
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1267_length_11827_cov_2.893646"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            979..1395
                     /gene="rpsL"
                     /locus_tag="PLC71_02570"
     CDS             979..1395
                     /gene="rpsL"
                     /locus_tag="PLC71_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRM01563.1"
                     /translation="MPTFNQLVRKGRKTSVKKSTAPALQKGFNSLKKKATDTSSPQKR
                     GVCTAVKTATPKKPNSALRKIARVRLSNGIEVTSYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGTRYHIVRGTLDTAGVANRMQARSKYGAKRPKKK"
     gene            1566..2036
                     /gene="rpsG"
                     /locus_tag="PLC71_02575"
     CDS             1566..2036
                     /gene="rpsG"
                     /locus_tag="PLC71_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRM01564.1"
                     /translation="MPRKGHIAKRDVLADPIYNNKVVTKLINNIMLDGKKGVAQKIVY
                     GAFARIEEEAGKPALEVFEEAMNNIMPVLEVKARRIGGATYQVPIDVRPDRRQALALR
                     WITTFSRKRGERTMEERLAKELLDASNNTGASVKKKEDMHKMAEANKAFAHYRF"
     gene            2105..4216
                     /gene="fusA"
                     /locus_tag="PLC71_02580"
     CDS             2105..4216
                     /gene="fusA"
                     /locus_tag="PLC71_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006782259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRM01565.1"
                     /translation="MAGREYPLERTRNIGIMAHIDAGKTTTTERILYYTGVNYKIGDT
                     HDGTATMDWMEQEQERGITITSAATTCHWTLQDHCKPKKGALEHRINIIDTPGHVDFT
                     VEVERSLRVLDGAVAVFCAKGGVEPQSENVWRQADTYNVPRMAFINKMDILGANFYGA
                     VDQMKDRLGANVAVLQLPIGKEDDFKGIIDLFEMEAYIYNDEKGEDISIVEIPEDMKE
                     EAELYHTELIEKICELDDDLMMQYLEGEEPSVDDLKAALRKGTCECKVVPVCCGTAYR
                     NKGVQKLLDAIIEFMPAPTDIPAIKGVDEDGNEVERHSSDDEPFSALAFKIMTDPFVG
                     KLAFFRVYSGTCKSGSYVLNATKNKKERVGRILQMHANKREELDIVYSGDIAAAVGFK
                     STTTGDTICDEQHPVILESMEFPEPVIDVAIEPKTKGDQGKMAEALAKLAEEDPTFRA
                     HTDHETGQTIISGMGELHLEIIVDRLLREFKVEANVGAPQVAYKETFTKAVDVDSKYA
                     KQSGGRGQYGHCKVHFEPMDANGEELFKFESTVVGGSIPKEYIPAVGEGIEEAAQSGI
                     LGGFPVLGVKATVYDGSYHDVDSSEMAFHIAGSMAFKEAMQKAGAILLEPIMRVEVTM
                     PEEYMGDVIGDINSRRGRIEGMDDIASGKMVRAFVPLSEMFGYSTDLRSRTQGRGNYS
                     MFFEKYEPVPKNVQEKVLADK"
     gene            4383..5576
                     /gene="tuf"
                     /locus_tag="PLC71_02585"
     CDS             4383..5576
                     /gene="tuf"
                     /locus_tag="PLC71_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRM01566.1"
                     /translation="MAKEKFERTKPHCNIGTIGHVDHGKTTLTAAITKVLAERVEGNT
                     AENFEDIDKAPEERERGITISTAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVAATDGVMAQTKEHILLSRQVGVPYIVVFMNKCDMVDDEELLELVEMEIRELLN
                     EYEFPGDDIPIIQGSALKAIEDPAGPWGDKIMELMDAVDSYIPDPQRDTDKPFLMPVE
                     DVFSITGRGTVATGRVESGVLHVSDEVEIVGIKEETRKVVVTGVEMFRKLLDEAQAGD
                     NIGALLRGVQRDEIERGQVLCQPGSITCHTKFTAQVYVLTKDEGGRHTPFFNNYRPQF
                     YFRTTDVTGVIELPEGTEMCMPGDNVEMTIELIHPIAMAQGLSFAIREGGRTVGSGRV
                     ATIIE"
     gene            5726..7405
                     /locus_tag="PLC71_02590"
     CDS             5726..7405
                     /locus_tag="PLC71_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM01567.1"
                     /translation="MNQTLKLPVGIENFEDIRKLGFYYIDKTKLIEQLLQNWGKVNLF
                     TRPRRFGKTLNMSMLRTFFEIGMDKSLFEGLYISKNKELCDEYMGKYPVIFLSLKGID
                     GLTFEEAIIRITTIIKNEARRHHYLKNSDKLIEEEIKQFQSLLDGKADDITDSIRLLS
                     ELLCKHYGKKTIILIDEYDVPLDKAFQKGYYDEMVELMRGLLGQVLKTNDFLQFAVLT
                     GCLRISKESIFTGLNNFEVLSILNVQYDECFGFTDTEVKKILSDYNLSDHYLQIREWY
                     DGYRFGNTDIYCPWDVIRYCKSLCADPDALPEDFWSNSSGNEIVRRFIDKADIQTKNE
                     IERLISGECIEKEIAEELTYNELDKSIENLWSVLFTTGYLTQQGRTQNGKYLLSIPNT
                     EIRNLFTKKIKEWFSDVSKNDGKTLEIFCNAFIEKKPEKIEQLFGDYLWNTISIRDTA
                     IAKEKKENFYHGILLGLLGYKSSWLIKSNAESGIGYSDILIEAPENRTGIVIELKYAE
                     DGNMDAACKQALKQIEDKDYIAKLKQDGMRNFIKYGVACYKKDCKVMIYEE"
     gene            7433..8212
                     /locus_tag="PLC71_02595"
     CDS             7433..8212
                     /locus_tag="PLC71_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM01568.1"
                     /translation="MGKETFEGKKTHCTIGIIGYRGHGKTTLTDAIKKTLTTKEERNI
                     SLTSSIEYGAILVVSAIDGIMSQTAEQIMIAHHMGMQHIVVFMNKCDMVDDMEMLELV
                     EMGIRDQLYQCGFVGSDIPFIYGSALKALEDPDGEWGDKIMELMDAVDKYIPDSQPEM
                     DQDSSIHTKFTAEVYMLDINEGGRDTGYFDDDRLQFYFKTTDVTGEIQLPIEIDMAMP
                     GETLDITIELIQPIKITEEEPFIIRDDECTVGLGRVATIIE"
     gene            complement(8355..8702)
                     /locus_tag="PLC71_02600"
     CDS             complement(8355..8702)
                     /locus_tag="PLC71_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM01569.1"
                     /translation="MKIDLKELGKRIKLTRKGQDLTQEMLAEKAGVSQHYIYEIEAGR
                     KAMSIHSFASLTTALDVSADYLLFGDGQRIFDVDTPAHHNNQLMEIAAELNPSQRDHV
                     LEILEVMVPYIKN"
     gene            8821..9414
                     /locus_tag="PLC71_02605"
     CDS             8821..9414
                     /locus_tag="PLC71_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5688 family protein"
                     /protein_id="HRM01570.1"
                     /translation="MRKNEKEGVMQRENERIIYHLMHFDEKDKEWSERPYLLLEDMAI
                     VFDILDLDDRSIQRIDHKKANKEQWSDQFLWESAKKNTKQFLPAKFEPVYKDFRGHTE
                     DRPVFMVSNKIQRYGAGVICYEDFLGDISRKYNKSLYLLPTSIHEMLLLFDDGEDQEE
                     DLLHILEKSDQQLSKEEFLSENIYYYDKYMGELISLF"
     gene            9441..10037
                     /locus_tag="PLC71_02610"
     CDS             9441..10037
                     /locus_tag="PLC71_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRM01571.1"
                     /translation="MIKKTDQTKKGVELMIPGIVIGVIVLVIVIWLISSYNGFVTLRN
                     KTEEAFSAIDVSLKKRYDLIPNYVETVKGYAKHESETLEKVVAARNQAMTATNIDDKI
                     KQENVLSGALKSLFAVSESYPELKANENFNKLQDQLQRLEEEIAGSRRYYNGVVNRYN
                     TKTELFPGNIIAGIFGFKRKPLFEVSNESERENVQVKF"
     gene            10061..>11827
                     /locus_tag="PLC71_02615"
     CDS             10061..>11827
                     /locus_tag="PLC71_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HRM01572.1"
                     /translation="MRKWITCTIMAVMMAAMIFMGTGNNVFANDEVTYDSNMQTKKFD
                     VDVKVGEDGSYTVTENIKVKFVNPRHGIYRYIPYKGKVITSDKNGDQKKLLYYADFTL
                     LSNDSKTDVDEDSDGNSQVLRFGSADYTVSKGNYRFTYQLIPKYQGDTYDYLYYNIFP
                     TLWRNKIPEGSTFTIHFPKKTDLKSVNFYYGRYGESKYAKDVITLKYDEQKNQIQGTL
                     KKTLPFKNGLTCYSDLGDGYFTAKHEIGVDRWIFGLSIGILVITAALLVLFGRDEKII
                     PSIQYQPPEGMDSAVVGYVLDGSVDDKDVISLILYWADKGYLKMKEKGQKDMEFIKLK
                     DIPDSEPRYQKTMFEALFKNRKKVKASSLQYKFADTVQVVKDDIKYDYKKNIYATSSK
                     VARIVSFVLLQLPICLFAFIMMIFSPDGILNLILPLMAWVLYFIGMFLACHSVDKWYA
                     ISKGARTGLPIASAVLSILGFIFYGLYYYVKIQRGELFDFFHVYLVIVAVSFIGVILT
                     AFMKKRTHQCVEWMGYLAGLRDFIETAELDRMKVLAKDHPDMFYHILPYSMVFGLFDL
                     YAKKLDALKLPAPDWYVYGGSGP"
BASE COUNT         4235 a         1813 c         2538 g         3241 t
ORIGIN      
        1 cgcagacaga taaccatttt tataatattc caccaacttc agtctatcaa actataatct
       61 gtttattgta tgtaaaatcc ttaagaaatc tgaaaaaact atttaattga caaagtaaac
      121 agatattgat ataataaaat caacaaatga aagataagtt ttaaaagaaa ggagaatcgc
      181 ccaagttgct ccataaggct aacggaaagg cttttaaaac cggcagctga tggatcagaa
      241 gtattaagat gattgggaat catgagactg actcaacagg atattatgat ctcgggagag
      301 aacataagag tcctagaagc ggatagaacg gtaaaggagt caacctcata aaaataatac
      361 gaagaataga gagaatgaac aagatcttaa gataagatca gaagatgatc aaagaaaaag
      421 aatccaaaca agaaagattc caagagacga ggaatccaaa caagaaagat tcagataaga
      481 gatcaacaaa cattcgatca gctgaccggt ggagcattta aaaaatttgt aactggtaac
      541 taaagcaaag tgtaacccgt taacaaaata atttggaact tattaactga atattttctc
      601 aagaataaga gattgatttt tatatattaa gaaaatggcg taaagaagcc atataacaaa
      661 gaaatcgaaa cattgagatc aagaaaagag atcagaatag aagctgtcag ggtcgatatc
      721 agagatgtga cgagaaagca tttctgaaga cagtgggata agaaaagatt tcgaataaga
      781 tccatagaat gagatttcca aaaagataaa aatatataga aattaataaa aatcttggga
      841 aaaggtcttg acaaaggcat gcaggcattt tataatagcc tagcatgcct ttttggggag
      901 caaaaccaag aagtgcatgt atagtcacaa ggtgaattta agtaaccaca gtagaaaaca
      961 tgtatggagg tgaaatcaat gccaactttt aaccaattag ttagaaaagg gagaaaaaca
     1021 tctgtaaaga aatctacagc accagcactt cagaaaggat ttaactcttt aaagaagaaa
     1081 gctacagaca caagctcacc acagaagaga ggtgtatgta cagctgttaa aactgcaact
     1141 cctaaaaagc ctaactctgc tttacgtaag atcgccagag ttcgtctttc taacggaatc
     1201 gaagtaacaa gttatatccc aggtgaagga cataacttac aggaacatag tgttgttctg
     1261 attcgtggtg gtcgtgttaa ggacttacca ggtactcgtt accatatcgt tagaggtaca
     1321 cttgatacag caggtgtagc taacagaatg caggctcgtt ccaaatatgg agctaaaaga
     1381 cctaagaaaa aataaaacaa aactaaattg gtacaaagca gcgtaattaa acgctacctt
     1441 gtatagttgt gccggaactt tattgtttcc tacaaaggtt acaggagata taaaagtcct
     1501 gcacgaacat gcaataacta agtatgagta ccgatgaatt aatgattatt aaggagggaa
     1561 gaaccgtgcc acgtaaagga catattgcga aaagagacgt attagcagat cctatttaca
     1621 acaataaggt tgtgacaaaa ttaatcaaca acattatgtt agatggtaaa aaaggagttg
     1681 ctcagaaaat cgtatacgga gcatttgccc gtatcgaaga agaagctgga aaaccagctt
     1741 tagaagtatt cgaagaagct atgaacaaca tcatgccagt tcttgaagta aaagctagac
     1801 gtatcggtgg agcaacatat caggtgccta tcgacgttag accagacaga agacaggcat
     1861 tagcgcttcg ttggatcaca actttctcac gtaaaagagg agaaagaaca atggaagaac
     1921 gtcttgcaaa agaacttctt gacgcttcca acaatacagg agcatctgta aagaaaaaag
     1981 aagatatgca caagatggca gaagcaaaca aagcattcgc acattacaga ttctaatttc
     2041 aagaatctgt tctggcgtct tgccagatat gcttctatgt taaaacttat aggaggaaaa
     2101 aatcttggct ggaagagaat atccattaga gagaaccaga aatatcggaa tcatggctca
     2161 tattgatgct ggtaaaacaa caacaacaga gcgtatcctt tattacactg gtgtaaacta
     2221 taaaatcggt gatactcatg atggtactgc tactatggac tggatggagc aggaacagga
     2281 aagaggtatc acaatcactt ctgcagctac aacatgtcat tggacactgc aggatcattg
     2341 caagcctaaa aaaggtgctt tagagcatcg tatcaatatc attgatactc cagggcacgt
     2401 tgacttcaca gttgaagtag aacgttctct gcgtgtactt gatggtgcag tagcagtctt
     2461 ctgtgcaaaa ggtggagtag agcctcagtc tgaaaacgta tggagacagg ctgatactta
     2521 taacgtacct agaatggcat tcatcaataa gatggatatc ttaggtgcta acttctacgg
     2581 agcagttgat cagatgaaag atcgtctggg agctaacgta gcagtacttc agttacctat
     2641 cggtaaagaa gacgacttca aaggaatcat cgacttattc gagatggaag cctacatcta
     2701 caatgatgaa aaaggtgaag acatctctat cgttgaaatc ccagaagata tgaaagaaga
     2761 agcagaactt tatcacacag aattgattga aaagatctgt gaattagacg acgatctgat
     2821 gatgcagtac cttgaaggtg aagaaccaag tgttgatgat ttaaaagctg cattaagaaa
     2881 aggaacatgc gaatgtaaag tggttccagt atgctgtggt acagcatata gaaataaagg
     2941 tgttcagaaa ttactggatg ctattatcga atttatgcca gctccaacag acatcccagc
     3001 tatcaaaggt gtcgatgaag atggtaacga agttgaaaga cattcatctg atgatgaacc
     3061 attctcagct ttagcattta agattatgac agacccattc gtaggtaaac ttgcattctt
     3121 ccgtgtttac tctggaacat gcaaatcagg atcttacgta ttaaatgcaa caaagaacaa
     3181 aaaagagcgt gttggacgta tcttacagat gcatgctaac aagcgtgaag aattagatat
     3241 cgtatattca ggagatatcg cagctgcagt aggtttcaaa tctacaacaa ctggtgatac
     3301 aatctgtgat gaacagcatc ctgtaattct ggaatccatg gaattcccag aaccagttat
     3361 cgacgtagcg atcgaaccta aaactaaagg tgatcagggt aaaatggctg aagcacttgc
     3421 taagctcgct gaagaagatc ctacattcag agctcataca gatcatgaaa caggacagac
     3481 aatcatttcc ggaatgggtg aacttcatct ggaaatcatc gttgaccgtc tgcttcgtga
     3541 attcaaagta gaagctaacg ttggtgctcc tcaggtagca tacaaagaaa catttacaaa
     3601 agccgttgac gttgacagca aatatgctaa acagtctggt ggtcgtggtc agtatggtca
     3661 ctgtaaagtt cactttgagc caatggatgc caatggagaa gaactgttca aattcgaatc
     3721 tacagttgtt ggtggatcta ttcctaaaga atatatccca gcagttggag aaggtatcga
     3781 agaagcagct cagtctggta tcttaggagg attcccagta cttggtgtta aagctacagt
     3841 atatgatgga tcttaccatg atgtcgattc tagtgaaatg gccttccaca ttgccggatc
     3901 tatggcattt aaagaggcta tgcagaaagc tggagctatc ttgcttgagc caatcatgag
     3961 agtcgaagtt acaatgcctg aagaatacat gggagacgtt atcggagata tcaactctcg
     4021 tcgtggacgt atcgaaggta tggatgatat cgcgagcggt aagatggttc gtgcattcgt
     4081 tccattatct gaaatgttcg gatattctac agacttacgt tctagaactc agggacgtgg
     4141 taactactct atgttctttg aaaaatacga accagtacca aagaacgtac aggaaaaagt
     4201 acttgctgac aagtaagaaa attaatatat aaatatttcg aggctatttt gtcaaaatag
     4261 tcttgaaata tttgtttaaa tgaaatataa tcaaatttag gacggccctt tgctgggcaa
     4321 ctatattgaa gtttgaaaca ttattgtaat tgcccatagg gcgaataagg aggacaatta
     4381 aaatggcaaa agaaaagttt gagagaacta aacctcattg caacattggt actattggac
     4441 acgttgacca tggtaaaaca actttaacag cagcaatcac taaagtttta gctgaaagag
     4501 tagaaggaaa cacagctgag aactttgaag atatcgataa agctccagaa gaaagagaac
     4561 gtggtatcac aatctctaca gctcatgttg agtatgaaac agaaaaacgt cactatgcac
     4621 acgttgactg cccaggccat gctgattatg taaagaacat gatcactggt gcagctcaga
     4681 tggacggagc tatcttagta gtagctgcta ctgatggtgt aatggctcag acaaaagaac
     4741 atatcctgtt atctcgtcag gtaggtgttc cttacatcgt agtattcatg aacaaatgtg
     4801 atatggtaga cgatgaagaa ttattagaat tagtagaaat ggaaattcgt gaattattaa
     4861 atgaatacga attcccagga gacgacattc ctatcatcca gggatctgct ttaaaagcta
     4921 tcgaagatcc agctggacca tggggagata agatcatgga attaatggat gctgttgaca
     4981 gctacattcc agatccacag cgtgatacag acaaaccttt cttaatgcca gtcgaagacg
     5041 tattctctat cacaggacgt ggtacagtag caactggtag agtagaaagt ggtgttcttc
     5101 acgtatctga cgaagttgaa atcgttggta tcaaagaaga aacaagaaaa gttgttgtaa
     5161 ctggtgtaga aatgttccgt aaactgttag acgaagctca ggctggagat aacattggtg
     5221 cattattacg tggtgttcag agagacgaaa tcgaaagagg acaggttctt tgccagccag
     5281 gttcaatcac atgccataca aaatttacag ctcaggtata cgttttaaca aaagatgaag
     5341 gtggacgtca tactccattc ttcaacaact atcgtccaca gttctacttc agaacaacag
     5401 acgttacagg tgttatcgaa ttaccagaag gaacagaaat gtgcatgcct ggtgacaacg
     5461 tagaaatgac tatcgaatta attcacccaa tcgctatggc tcagggatta tctttcgcta
     5521 tccgtgaagg tggacgtaca gtaggatcag gaagagttgc tacaatcatc gagtaattct
     5581 tacttctacg attgaattta ggataatatt tatgagactc agaaagttta ggctttctgg
     5641 gtcttttttg ttgtgagcaa atataaaaca agctataata aaagagacac attggtcgta
     5701 aggcaaaatt aataggagga tccgaatgaa tcaaacatta aagctgccag ttggaattga
     5761 gaattttgaa gatattcgca agctaggatt ttattatata gataagacaa aattaattga
     5821 acagctctta caaaattggg gaaaagtaaa tctgtttaca cgaccacgtc gttttggaaa
     5881 gacactcaat atgagtatgt tacgtacttt ttttgaaatt ggtatggata aatcactttt
     5941 tgaaggccta tatatttcta aaaataaaga attatgtgat gaatatatgg gtaagtatcc
     6001 agtaatcttt ctgtcattga agggaatcga tggattaact tttgaagaag cgattattcg
     6061 gattacaacg attattaaaa atgaagcacg aagacaccat tatctaaaaa atagtgataa
     6121 attaatagaa gaagagatta agcaatttca gagcctttta gatggaaaag ctgatgatat
     6181 aacagatagt atcagactat tatcagaact tttgtgcaaa cattatggga agaagacgat
     6241 tattcttatt gatgaatatg atgttccgct agataaagct tttcagaaag gctattatga
     6301 tgaaatggtt gaactcatga gaggtctttt ggggcaagta ctaaaaacaa atgattttct
     6361 acaatttgca gttttgacag gatgtcttag aatttcgaaa gaaagcattt tcaccggact
     6421 taataatttt gaagttttat ctattctcaa tgttcagtat gatgaatgtt ttggatttac
     6481 cgatacagaa gtgaaaaaga ttcttagcga ttataattta tcagatcatt accttcagat
     6541 tagggagtgg tatgatggtt atcgtttcgg aaatacagat atctattgcc catgggatgt
     6601 gattcgttat tgtaaaagtc tatgtgcaga tcccgatgca ttaccagaag atttttggtc
     6661 taattctagt ggaaatgaaa ttgttcgtag atttatagat aaggcagata ttcagacaaa
     6721 gaatgagatt gagcgtttga tcagtggaga gtgtattgaa aaggaaattg cagaggagct
     6781 tacctataat gagttggata agagtataga aaatttgtgg agtgtgcttt ttacaacagg
     6841 atatctaaca caacagggac gaactcaaaa tggtaaatat cttttgtcga taccaaatac
     6901 agagataaga aatctattta caaaaaagat caaagaatgg tttagtgatg tttcaaaaaa
     6961 tgatggaaaa acactagaaa tattttgtaa tgcgtttata gagaaaaaac cagaaaaaat
     7021 tgaacagctt tttggagatt atctatggaa tacgatcagt ataagagata cagcaatcgc
     7081 aaaagagaag aaagaaaatt tttatcacgg aattttactc ggtcttcttg gatataagtc
     7141 aagctggctg ataaagtcaa atgcagaatc aggaatcgga tacagtgata ttctgataga
     7201 ggctccggag aatcgaacgg gaatcgtcat tgaattaaag tatgccgaag atggaaatat
     7261 ggatgcagcg tgcaagcagg ctttaaaaca gattgaagat aaagactata ttgctaaatt
     7321 gaagcaagat ggaatgcgaa atttcattaa atatggtgtt gcttgttata agaaagactg
     7381 taaagtcatg atttatgaag aataaaaaca actgatgatg ggagatataa atatggggaa
     7441 agaaacattt gaaggaaaga aaactcattg cacgatagga ataattggat atcgaggtca
     7501 tggaaaaacg actttaacag atgcaatcaa aaaaactctt acaacaaaag aagagagaaa
     7561 tataagcttg acatctagca ttgaatatgg agctatccta gtagtatctg caatagatgg
     7621 aatcatgtca caaacagcag aacagattat gattgctcat catatgggaa tgcaacatat
     7681 cgtagtattt atgaataaat gtgatatggt agatgatatg gaaatgctgg aattggtaga
     7741 aatggggatt cgtgaccagc tatatcagtg cggatttgtt ggatctgata ttccgtttat
     7801 ctatggatct gctttaaaag cccttgaaga tccagatgga gaatggggag ataagatcat
     7861 ggaattgatg gatgcggtgg ataaatacat tccagattca cagcctgaaa tggatcaaga
     7921 ttcctctata catacaaagt ttacagcaga agtatacatg ttagatatca atgaaggtgg
     7981 acgagacaca ggatactttg atgatgaccg tctgcagttt tatttcaaaa cgacagatgt
     8041 aacaggagag attcaattgc caatagaaat agatatggct atgccagggg aaacactgga
     8101 tataacaatt gaattaattc aaccaattaa gataacagaa gaagaacctt ttatcattcg
     8161 tgatgatgaa tgtacggttg gattgggaag agttgctaca atcatcgagt aattcttaca
     8221 ctattcttca tggagtagtg taaaaaaagt aaaaatcaaa aaataaatat cagatcaaat
     8281 aaaaatagtg tcaagatcaa taaaaatctt ggcactattt tacgttagaa aaatctatcg
     8341 tattttgcaa tcaatcaatt tttaatataa ggaaccatca cttccagaat ctcaagcaca
     8401 tgatctctct gtgatggatt aagttcagct gcaatttcca tcaattgatt attgtgatga
     8461 gccggggtat caacatcaaa tattctttgc ccatcaccaa ataaaagata atcagcagat
     8521 acatccaatg cagtagtaag agaagcaaaa ctatgaatag acatggcttt gcgtccagct
     8581 tcgatttcat agatataatg ctgactgaca ccagcttttt cagcaagcat ttcctgtgtc
     8641 agatcttgcc cttttcgggt aagtttgatg cgttttccta attcttttaa atcgattttc
     8701 ataaaatacc tccataaaaa atagtattac caggagttat gaaaaaccac tatccaatag
     8761 tgacagtgac gactataaat attgctgaca gcaacattga atgttatgat agataggaaa
     8821 atgagaaaaa acgagaaaga aggagttatg cagagagaaa acgagcgtat tatctatcat
     8881 ttgatgcatt ttgatgagaa ggataaggag tggagtgaga gaccatattt actcttggaa
     8941 gatatggcaa ttgtttttga tattcttgat ctggatgatc gatccataca aagaatcgat
     9001 cataaaaagg caaacaaaga acaatggtcg gatcagtttt tatgggagag tgcaaagaag
     9061 aatacaaaac agtttctgcc tgctaaattt gagcctgtat ataaagattt tcgaggacat
     9121 accgaagaca gaccggtatt tatggtgtca aacaaaatac agagatatgg agcaggtgtg
     9181 atttgctatg aagattttct aggcgacatt tcaagaaaat ataataagag tttgtatctc
     9241 ctgccaacca gtattcatga aatgttatta ctgtttgatg acggagaaga tcaggaagag
     9301 gatctattgc acatattaga gaagtctgat cagcaattaa gcaaagaaga atttctgtca
     9361 gaaaatatct attattatga taaatatatg ggagagttga tcagcttatt ttagtgttga
     9421 agttttgata cgaataacgt atgataaaga aaactgacca aacaaagaaa ggggtagagc
     9481 tcatgatacc agggatcgta atcggagtga tcgtgcttgt cattgtgata tggctgatca
     9541 gttcctacaa tggatttgta acattaagaa ataagacaga agaggcattt tcagcaatcg
     9601 atgtgtcatt aaagaagcgt tatgacttga ttccaaacta tgtagaaacc gtcaaaggtt
     9661 atgcgaaaca cgaatcagag acattagaga aagtcgtagc cgcaagaaat caggcgatga
     9721 cagcgacaaa tatcgatgat aagatcaaac aggaaaatgt actgtcagga gcactgaaat
     9781 ctttatttgc agtgtctgaa agctatccag aattaaaagc taatgagaac tttaataaat
     9841 tgcaggatca gcttcagaga ttagaagaag agatcgcagg ttccagaaga tattataatg
     9901 gagttgtaaa ccgctataat accaagacag agttattccc aggcaatatc atcgcaggaa
     9961 tctttggatt taagagaaaa ccattatttg aagtttcgaa tgaatctgag cgtgagaatg
    10021 tacaagtaaa attctaagga acggacaggg ggaaagcact atgagaaagt ggatcacctg
    10081 taccataatg gcagtcatga tggctgccat gatttttatg gggacaggaa acaatgtgtt
    10141 tgcaaatgat gaagtcactt atgactctaa tatgcagaca aagaaatttg atgtagatgt
    10201 caaagtcgga gaagatggaa gttatacagt aacagagaat atcaaagtca agtttgtcaa
    10261 tccgaggcat ggaatttatc gttatatccc atacaaaggg aaggtgataa caagcgataa
    10321 aaatggagat cagaaaaaac tactttatta tgcggatttt acactgttat caaatgacag
    10381 taagacagat gtagatgaag attctgacgg taatagtcag gtattgaggt ttggttctgc
    10441 ggattatacc gtaagtaaag gaaactatag atttacgtat caattgatcc ctaaatatca
    10501 gggagatacc tacgattatt tgtactataa tattttccca acactctgga gaaataagat
    10561 tccagaagga agtactttta cgattcattt tccaaagaaa acagatctta agagtgtaaa
    10621 tttctattat ggacgttatg gggaatcaaa gtatgcaaaa gatgtgatca ctttgaaata
    10681 tgatgagcag aagaaccaga tccaaggaac attaaagaag acactgccat ttaaaaatgg
    10741 attgacatgc tatagtgatc tgggtgatgg gtattttaca gcaaaacatg aaatcggagt
    10801 agatcgatgg atttttggac tatcaatagg aattcttgtg atcacagctg ccttacttgt
    10861 tttgttcgga cgtgatgaaa agatcatccc atcgattcaa tatcagccgc cagaaggaat
    10921 ggacagtgca gttgtcggat atgttctgga cggttctgtg gatgataagg atgttatttc
    10981 tttgatcctt tattgggccg ataagggcta cctgaagatg aaggaaaaag gtcaaaagga
    11041 catggaattt atcaaactca aagatattcc agactcagaa ccaagatatc agaaaacaat
    11101 gtttgaagct ttatttaaga acagaaaaaa agtgaaagct tcttctttac agtacaaatt
    11161 tgcagatacc gtgcaggtag tgaaagatga tatcaaatat gattataaga aaaatattta
    11221 tgcaacatcc tcgaaagttg caaggatcgt atcctttgta ttgttgcagc ttccaatctg
    11281 tttatttgca tttattatga tgatatttag tccagatggg atcttaaatc tgatccttcc
    11341 attgatggcg tgggtattgt attttatagg aatgtttctt gcatgtcaca gtgtagataa
    11401 atggtatgcg atttcaaagg gagcaagaac gggacttccg atcgcatcgg cagtattgtc
    11461 tattcttgga tttattttct atggattata ttattatgtg aagatacaaa gaggagaatt
    11521 atttgatttc ttccatgtgt atcttgtgat cgttgcagta tcatttatcg gagtgatcct
    11581 gactgcattt atgaagaaga gaacccacca atgtgtggaa tggatgggat atctagcagg
    11641 acttcgtgat tttattgaaa ctgcggaatt agaccgaatg aaggtattgg cgaaagatca
    11701 tccagacatg ttttatcata ttctgcctta ttccatggta tttgggttgt ttgatcttta
    11761 tgcaaagaaa ctggatgcat taaaacttcc tgcgccagac tggtatgtct atggcggcag
    11821 tggtcct
//
LOCUS       DAOMUE010000029        11744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1288_length_11744_cov_3.143212, whole genome shotgun sequence.
ACCESSION   DAOMUE010000029 DAOMUE010000000
VERSION     DAOMUE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1288_length_11744_cov_3.143212"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLC71_02620"
     CDS             <1..225
                     /locus_tag="PLC71_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRM01573.1"
                     /translation="LPHSSISQRSYGTAISWANKQPGDLICYNAINGIGHVGIYIGNN
                     RVIHAGSTATGIHTSVANYRSVNCVRRIVR"
     gene            487..1332
                     /locus_tag="PLC71_02625"
     CDS             487..1332
                     /locus_tag="PLC71_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRM01574.1"
                     /translation="MSSHTHDVYASYSRYVTANNVVVRKKASNKGKIVGSYKKAAKVR
                     CYKKKRSYTKIKYGRYYRYIATRYLSKKKPVVVTAQKAANITTDTYTRYVTASSLTIR
                     KKASTSAAKAGSYKKGTQITCYGERSGWTTVRYSGVYCYVSSQYLSESKPEEDTWEVY
                     GTATGQSVVDYAMQFRGNPYVWGGESLTNGVDCSGFTMQVYKHFGYSLPHSSTAQRYE
                     GTAVSWNEKQPGDLICYQVVNGVGHVAIYIGDNQIIHAGSKDTGINVRNADYRAVWGV
                     RRIVQ"
     gene            complement(1407..>2462)
                     /locus_tag="PLC71_02630"
     CDS             complement(1407..>2462)
                     /locus_tag="PLC71_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRM01575.1"
                     /translation="VFVEGIIFIVLSLTNVREAIFDAIPLNLKKGVSVGIGIFIAFIG
                     LQNAKLVIGNKSTLVSITNFTKDFHTAGICSLLAVIGLLITVILYIKKVPGSILIGIL
                     ATWVIGMLCQITGIYVPDFKTGYYSLFPTFAMTDFSKLGETFGKCFQYDLGKVGIFNF
                     IAVVLSFLFVDLFDTLGTLVGVSTKAGMLDEEGKLPGIKPALMADAVATTAGAVLGTS
                     TVTTFVESSSGVAAGGRTGLTAVVSGFLFLISTLFAPLFTAIPSFATAPALIMVGFLM
                     FGAISDIKFTDDNMTEAVPAYLCIIAMPLFYSISEGISIGIISYVILNVVCGKAKKIT
                     PLMYVLAVLFILKYAVL"
     assembly_gap    2463..2472
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2473..2782)
                     /locus_tag="PLC71_02635"
     CDS             complement(<2473..2782)
                     /locus_tag="PLC71_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRM01576.1"
                     /translation="MLENLFKLKENHTSVKTEVIAGITTFMTMAYILAVNPSVLSAAG
                     MDPTAVLLATCIASFIGTLCMGLTANLPFVLSAGMGLNAYLAYTVVGVMGYHWQVALL
                     A"
     gene            3250..6460
                     /gene="carB"
                     /locus_tag="PLC71_02640"
                     /pseudo
     CDS             3250..6460
                     /gene="carB"
                     /locus_tag="PLC71_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            7180..7683
                     /locus_tag="PLC71_02645"
     CDS             7180..7683
                     /locus_tag="PLC71_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRM01577.1"
                     /translation="MEERWDIYDKNKQLTGRTMKRNDWTLKDDEYHLTVLGVIRRSDG
                     KFLITKRVMTKAWAPGWWEVSGGAAQAGEASYDAVLREVKEETGLDVKDAEGGYMFTY
                     KRENPGEGDNYFVDVYRFTLDIDDSDVNFQEAEIDGYMFASIDEIKVFAQEGIFLHYD
                     SIKKVFE"
     gene            7688..9061
                     /locus_tag="PLC71_02650"
     CDS             7688..9061
                     /locus_tag="PLC71_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM01578.1"
                     /translation="MSSTIKRNKYEIDMCNGTIMDKLISFSLPLMLSGILQLMFNAVD
                     IIVIGRFSGSQSLAAVGSTTALINMFTNLFIGISLGANVLSARFYAAGKDKEMSETVH
                     TAITLALISGIIMVFVGLIFAKPVLELMDTPDDVINLSTLYMRIYFMGMPFFMLYNYG
                     ASILRAVGDTKRPLFYLIVAGIANAGLNMCLVIIFHLGVAGVAIGTVISQLISSILAL
                     RCLYRSETSYQLRFSKLKIQKEYLKQIFQVGIPAGIQSTVINFSNVLLQSSVNSFGSI
                     AMAGYTAANNIFGFLYVTVNAVTQACMSFTSQNYGVGKWKRMDRVLIDCLILSFVAMM
                     ILGNSAYFFGPKLLTIYTSNSKVIQCGMEILLYTTVTYFLCGFMDLFPGALRGMGRSG
                     VPMILSIIGTVGTRIVWIFWIFPNHRSLDILFISYPASWIITIVLQVICYYFVRKQLY
                     AKMRAEA"
     gene            complement(9139..9369)
                     /locus_tag="PLC71_02655"
     CDS             complement(9139..9369)
                     /locus_tag="PLC71_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01579.1"
                     /translation="MFGKRKKRREAIRNKLREKSEYKMKSENVAIPEIHTKDFDEIEE
                     HIDEIEQEKEEKHSILYDNVAIPEVHIHKKEK"
     gene            complement(9391..10578)
                     /gene="gltS"
                     /locus_tag="PLC71_02660"
     CDS             complement(9391..10578)
                     /gene="gltS"
                     /locus_tag="PLC71_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HRM01580.1"
                     /translation="MTIQLDMYQTIAVAVVVLMLGNFLKHRIAILERFCIPAPVIGGV
                     IFAIFTCVCYVTGFAEFSFDDILKEVCMVFFFTSVGFQANLKVLKSGGKSLIIFLILV
                     IMLIICQNFLAVGLSKALQISPLVGLCTGSIPMIGGHGTAGAFGPVLEDFGVKGASTL
                     CTAAATFGLIAGSIMGGPVGKRLIEKKNLLKTAIPEDNSLLIEEEKKHERHTSMYPAA
                     VFQLIIAMGIGTIISKLLSMTGMTFPIYIGAMIAAAFMRNIGEYSGQFTIYMGEINDI
                     GGISLSLFLGIAMITLKLWQLADLALPLITLLAGQTILMFLFTYFVVFNIMGRDYDAA
                     VLSSGVCGFGMGATPNAMANMQAICERYVPSVKAYLLVPLIGSLFADFLNSLVITFFI
                     NFL"
     gene            complement(10597..11592)
                     /locus_tag="PLC71_02665"
     CDS             complement(10597..11592)
                     /locus_tag="PLC71_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRM01581.1"
                     /translation="MRGDILKKKLMVLCAVAIMISTCLTGCKMNSGKIRFGSAGIGGT
                     YQIFGDTFANLISSKSKKYNIEVKTTAGSAANLRLLSKDYIQMAVAQMDLINDAYNRT
                     GIFENDKKYQGYRAVASLYTEACQIVVPADSDIQSMDDLEGKKVCIGEEESGTEQNAL
                     QILNAYGLNERLVDTVNLNYTDAAKKLKSGDIDAFFCTAGVQTTVINELSKQCKIRLV
                     SLDQKGVSRLKKSYKFYTEYTIPAGTYVNQTKEIKTVGVKAVLLASDKLSEDTVKDIT
                     QILFKNQQQIQYALPVDISLDETTAVDGITIPFHDGAAAYYKQHGITVNTEKGSN"
BASE COUNT         3960 a         2060 c         2430 g         3284 t
ORIGIN      
        1 cttccacaca gttccatttc ccagagaagc tatggaacag caatttcatg ggcgaataag
       61 cagccgggag atctgatctg ctataatgcg atcaatggaa tcggacatgt tggaatttat
      121 attggaaata atcgtgtgat tcatgcagga agcacggcga caggaataca tacatcagta
      181 gcaaattaca gatcagtaaa ttgtgttcga agaattgtaa gataatgtag ttaaaaaata
      241 ataaggaaga aggcatgcag acgatcaaag atttgattca aaacgatgaa aaatgttgca
      301 aataggtaca tttttcataa gaatttatga aaaaaatatt acgaaatggt ttacatatga
      361 aaaatgaggt agtaaactag aagtagtcgg taaaaaatgg aactaaaggg aggaaatatg
      421 aagagaaaga ctactggaat cctcatttcc tgtgtagtaa gtatatgttg tatggtggtc
      481 ttatccatga gttcacatac acatgatgta tatgcttcct attcaagata tgtaacagcc
      541 aataacgtag tggttcgtaa aaaagcatca aataagggaa agatcgttgg atcatacaag
      601 aaagcagcaa aggttcgctg ctacaagaag aaaagatctt atacgaagat caaatatgga
      661 agatattatc gatatatcgc aacaagatat ctgtcaaaga agaaaccagt tgttgtaaca
      721 gcacagaaag cagcgaatat aacaacagat acatatacaa gatatgtaac ggcaagttct
      781 ttgacgatcc gtaagaaagc gtcgacaagt gcagcaaagg caggatcata caagaaaggt
      841 acgcagatca cctgttatgg agaacggtct ggatggacaa cggtaagata tagtggagtt
      901 tattgttatg taagttcaca gtatctatct gagtcaaaac cggaagaaga tacatgggaa
      961 gtttatggaa cggcaacagg acaaagtgtt gtagactacg caatgcagtt tagaggcaat
     1021 ccttatgtat ggggtggaga aagccttacg aatggagttg actgttctgg attcacgatg
     1081 caggtatata aacattttgg atattcactg ccacacagtt caacagcaca gagatatgaa
     1141 ggaacagcag tatcatggaa tgagaagcag ccaggagatc tgatctgcta tcaggtagtg
     1201 aacggtgtag gacatgtagc gatctacatt ggagataacc agatcattca tgcaggaagc
     1261 aaagacacag gaatcaatgt gcggaatgct gattacagag ctgtatgggg tgtcagaaga
     1321 atcgttcagt aggacaagca ttttaattta atatgagata agaataaaac agccgtatcg
     1381 atcatgatac ggctgttttt ctatacttat aaaacggcat atttcaggat gaataatact
     1441 gcaagtacgt acattaaagg agtgatcttc tttgctttac cacaaactac attcaagatc
     1501 acataagaga tgatcccgat agagattccc tcagagatac tatagaataa tggcattgca
     1561 atgatgcaaa ggtaagcagg tacggcttct gtcatattgt catctgtaaa tttgatatca
     1621 gagattgctc caaacattaa gaacccaacc atgatcagcg caggggctgt cgcaaatgat
     1681 gggattgctg taaagagcgg tgcaaataat gttgagatca ggaataagaa tcctgaaaca
     1741 acagctgtta aaccagtacg tccacctgca gcaacaccag aagaagattc aacgaatgtt
     1801 gtaacagtag aagtaccaag aaccgcacca gctgttgtag caacggcgtc tgccattaat
     1861 gcaggtttga ttccaggaag tttaccttcc tcgtcaagca ttccagcttt tgtgctgaca
     1921 ccgactaaag ttccgagtgt atcaaacaga tcgacaaata agaaagataa tacaacggcg
     1981 ataaaattaa agattccgac tttgcccaag tcatactgga aacatttacc gaaagtctct
     2041 cctaattttg aaaaatctgt cattgcaaat gtagggaaca ggctgtaata acctgttttg
     2101 aaatcaggaa catagattcc tgttatctgg cagagcattc cgatcaccca tgtagcaagg
     2161 attccgatca ggatagatcc aggtactttt ttaatataaa gaatgactgt gatcaataat
     2221 ccaataactg caagtaaaga acagattcca gcagtatgga aatcttttgt aaagtttgtg
     2281 atagatacca atgtagattt atttccaata acaagttttg cgttctgaag accgataaag
     2341 gcaataaaga taccgattcc gacagaaact cccttcttaa gattcaaagg gatcgcatca
     2401 aagatcgctt cacgaacatt ggtcaaagat aatacgatga agatgatacc ttcaacgaat
     2461 acnnnnnnnn nncagcaagt aaagcaacct gccagtgata acccatcaca ccaacaacgg
     2521 tataggcaag ataagcattt aatcccatac cagcggaaag tacgaatgga aggttcgcag
     2581 taagtcccat gcataaagtt ccgataaagg aagcgataca tgtagctaat aatacagcag
     2641 tagggtccat gccagctgca gaaagcacac ttgggtttac cgcaaggatg taagccattg
     2701 tcataaatgt agtaatacca gcaatgactt cagtttttac ggaggtatga ttctccttta
     2761 atttaaataa gttttccaac atagtttttt tccgtatgta aaaacatacg ttcctctctt
     2821 tctttattga aaatctataa aaaagtaaca aacatcatta tattataggc aaaaatgaaa
     2881 agcaaggcgg gaaaatctga caaaaataaa aggaaaccat ctggggtgtg atggtttcct
     2941 ttttgaaaag agaggattaa aaatatatat gaaaaaacta tacctgtagg atgtttcctg
     3001 aaattctttt attgtcttat cttttaggtc tttccttgag gtatggttat agtataatca
     3061 tttccatttc gagattcaat atacagaata taaacaaata taaacgattt ataaattgtc
     3121 attaagaaat tcttacgaat tggtaagaaa atccaaagtt aacatagtta aaaaaacttc
     3181 acaaaatgat tccgatttca ctgattttct tatataataa taataaagtc taaaggagga
     3241 attattttta tgccaaaaag aacagatatt aacaaagtgt tgatcattgg ctctggtccg
     3301 atcatcatcg gtcaggcatg cgaattcgac tattcgggga cacaggcgtg taaggcgctt
     3361 cgtaaacttg gatatgagat cgtattggtc aactccaatc cagcaacgat catgacagat
     3421 ccagagacag ctgacgaaac ttacattgaa acgcagaacg tttagaggag atcattgcaa
     3481 aggaacgtcc agatgcttta cttccaaact taggtgggca gtccggatta aatttatgcg
     3541 aagagttata taaaaagggt attttagata agtataatgt caaagtaatt ggtgtacagg
     3601 tagatagtat tgaacgtgga gaagaccgta tcgagtttaa gaagacaatg gatgaacttg
     3661 gaatcgagat ggctcgaagc gaggttgctt acagcgtaga agatgggttg aagatcgctg
     3721 ataagcttgg ttatccagtg gtacttcgtc cagcttacac aatgggtgga gccggtggag
     3781 gactggtata taacaaagaa gaattaaaga ccgttatggc aagaggactt caggcaagtt
     3841 tagttggaca ggtacttgta gaagaatcca ttcttggatg ggaagaatta gaattagaag
     3901 ttgtccgtga tgcagaagga aatatgatca cagtctgctt cattgagaat atcgatccat
     3961 taggagtgca tacaggagac tccttctgtt ccgcaccgat gctcacgatc tctgaagaat
     4021 gtcagcagag attacaggaa caggcataca agattgtaga ttctgtacag gtgatcggtg
     4081 gaacaaatgt acagtttgct catgatccag tgacagaccg tattattgtt atcgagatca
     4141 atccaagaac atcaagatcc tcagcattag catccaaagc gacaggattc ccgatcgctt
     4201 tagtatctgc aatgcttgca gcaggtttaa cattaaaaga tattccatgt ggaaaatacg
     4261 gaacattaga taaatatgta ccagacggtg actatgtcgt tatcaagttt gcacgttggg
     4321 catttgagaa attcaaaggt gtcgaagaca aacttggaac acagatgcgt gccgttggtg
     4381 aagttatgag tatcggtaag acatacaaag aagcattcca gaaagcgatc cgaagcttag
     4441 agacaggacg ctacggactt ggacatgcga acaactttga tactttaaca aaagaagaat
     4501 tattaaagaa attagtgtca ccatcaagcg aacgtcattt catcatgtac gaagcattaa
     4561 gaaaaggtgc aactgtcgat gagatctttg aactgacaaa agtaaaaaca tactttatcg
     4621 agcagatgaa agagttagtt gaagaagaag aaaagcttct tgcatgcaaa ggaaacatgc
     4681 catctgatga aatgttaaca tcagcgaaga aagatggttt ctctgacaaa tatttaagtc
     4741 agttattaga gattccagaa gaagatatca gaaataaacg tatttcaatt ggtgtcgaag
     4801 aagcatggga aggggttcat gtaagcggaa cgccagacag tgcatattat tattcaacat
     4861 acaatgcaga agacaagaac ccagtatcta cagataaaca aaagatcatg atcttaggtg
     4921 gaggtccaaa ccgtatcgga cagggtatcg aatttgacta ctgctgtgta catgcatctc
     4981 aggcactgaa gaaaatggga ttcgagacga tcatcgtaaa ctgcaaccca gagacagtat
     5041 ctacagatta cgatacatcc gataaattat atttcgaacc attaacagtg gaagatgtat
     5101 taagtattta taataaagaa aaaccattag gagtgatcgc tcagtttggt ggacagacac
     5161 cactgaatat tgctgccgaa cttgagaaaa atggtgtgaa gattcttgga acatctccat
     5221 ctgtcattga ccttgcagaa gaccgtgatc tgttccgtga aatgatggat aaattagaga
     5281 ttccaatgcc ggaatcagga atggcaacaa cagtagaaga agcattagag atcgcaggaa
     5341 agattggata tccagtgatg gtaagacctt cctacgttct tggtggacgt ggaatggaag
     5401 ttgtctatga tgacgaaagc atgcagcagt aggggtaaca cctgatcgtc cgattttaat
     5461 tgaccgtttc ttaaatcatg cgatggaatg tgaagcagat gcgatcagtg acggaacaca
     5521 cgcatttgta ccagcagtga tggaacatat cgaacttgca ggagttcatt caggagactc
     5581 cgcttgtatt cttccatctg ttcatatttc agaagaaaac ctggaaacga tcaaagaata
     5641 cacaagaaag atcgcagaag aaatgcatgt caaaggacta atgaatatgc agtatgcgat
     5701 cgaagatgat aaagtttatg tattagaagc aaacccacgt gcatctcgta cagtaccttt
     5761 ggtatccaaa gtatgtaacg tgagaatggt tccactggca acacagatca tcacatctga
     5821 attaacagga aaaccatcac cagtaccaga attaaaagaa caggcaattc catattatgg
     5881 agtcaaagaa gcagccttcc cattcaatat gttccaagaa gtagacccag ttctaggacc
     5941 agaaatgcgt tctacaggag aagttcttgg attatccaaa tcctatggag aagctttcta
     6001 caaagcacag gaaggtgttg gagcgaaact tccattagga ggaacggtac tgatttctgt
     6061 aaatcgcaag gataaagaag aagttgtaga agtagcaaaa gcattcgcag acgacggatt
     6121 caagatcctt gcaacagaaa acacatgtaa actgatcaaa gaagctggaa ttgaagctga
     6181 gaaagtcaat aaattaagcg aaggaagacc aaacatcctt gaccttatca caaatggtaa
     6241 tatcgatatt attgtaaact ctccagtcgg aaaagacagc gtgaacgatg atagttacct
     6301 aagaaaaggt gcgatcaaag cgaagattcc atattataca acgatcgcag cagccaaagc
     6361 agcagctgaa ggaatcgctt atgtaaaagc acatcaggat cagaatgaag aagtgaattc
     6421 attacaggga ttacatgcac agatttctga taagttttaa tttgtaagtt ggccaactgt
     6481 agtctttcag tatatgataa aaaataaata aacgattgga agagcagcta gagaattttg
     6541 ctcacgtttt ttgcagatcg ctgatccgcc ggctttcctt gtgcaactcg ccttcaaggg
     6601 tgtttaaatc agaactcttt gaggctcaga cagcacacca agccggctgc gaatccgcaa
     6661 aaaacgtggc aaaattctaa tgctgctctt ccgatcgttt atttattttt tatcatatac
     6721 cagaagtcta aagtgtggac aattacaaga ttgaaaaatt caagtggggg tgtatggaga
     6781 acagcattga ttgtaaaata ggaaattttc agacagaagg tgcttgtaga ttttagctaa
     6841 acttctgtta acgaatacaa aaattatcaa tcaataccat acaacataca cactgtatag
     6901 tggtttgaat cacgtaaatt atttgaatat tataaaaaga gatcatgtgt cggcaaccgt
     6961 acggacacgt ctgcacttag cgagtggatg gtcgttagac cattcaggag cttagtacag
     7021 ctacgtggga gtccgaacgc aagtgggttg cagacacatg atctcttttt ataatattcc
     7081 accaataatt tccgtcttca aactacaaat cacaatttgc aataaatttc gtaaaatgtt
     7141 aagattaata aagaaacaca cccaaggaga aatgcaaaaa tggaagaaag atgggacatt
     7201 tacgataaga ataaacaact gacaggccgt acaatgaaga gaaatgactg gacattaaaa
     7261 gatgatgaat atcatctgac agttcttgga gtcattagaa gatcagatgg caaatttttg
     7321 atcacaaaga gagttatgac aaaggcatgg gcaccaggat ggtgggaagt gtctggtggt
     7381 gctgcacagg caggggaagc atcttatgat gcagttcttc gtgaagtgaa agaagaaaca
     7441 ggactggatg ttaaagatgc tgaaggcgga tatatgttta catacaagag agagaatcca
     7501 ggagaaggag ataactattt tgtagatgtt tatcgtttta cattagatat cgatgacagt
     7561 gatgtaaact ttcaggaagc agagattgac ggctatatgt ttgcctcaat agatgagatc
     7621 aaagtatttg cacaggaagg aattttcctg cactatgaca gtataaaaaa ggtatttgag
     7681 taaggcaatg agttcaacaa taaaacgtaa taaatatgaa attgatatgt gcaatggaac
     7741 gattatggat aaactgatct cattttcgtt accattgatg ttatccggaa ttttgcagct
     7801 gatgtttaat gcagtggata ttatagttat tggaagattt agtgggagcc agtcgcttgc
     7861 agcggttgga tcaaccactg ccctgatcaa tatgtttacc aatttattta ttggaatatc
     7921 ccttggagcg aatgttttat ccgcaagatt ttatgcggcg ggcaaagata aagaaatgtc
     7981 tgaaaccgtt catacggcaa tcacactggc gctgatcagc ggaattatta tggtctttgt
     8041 aggattgatc tttgcaaaac cagtattaga attaatggat acaccagatg atgtgatcaa
     8101 tctttccact ctttacatga gaatttattt tatggggatg ccgtttttta tgctttataa
     8161 ttatggagca tccattttaa gagcggtcgg agatacaaaa cgtccgctgt tttatttgat
     8221 cgttgctgga attgcaaatg caggtcttaa catgtgtctg gttatcattt ttcatcttgg
     8281 cgttgcagga gttgcaatcg gaacggtaat ttcacagctg atttcaagta tacttgcgct
     8341 tcgatgtctg tatcgatcag aaacaagtta ccaactgcgt ttttcaaaat taaagattca
     8401 aaaggaatat ttaaaacaga tttttcaggt gggaattcca gcaggaattc aaagcactgt
     8461 tattaatttt tctaatgtat tattgcagtc atcagtgaat tcttttggat caatcgcaat
     8521 ggcaggatat acggcagcaa ataatatttt tggatttttg tatgttacag tgaatgcagt
     8581 gacacaggct tgcatgagtt ttaccagtca gaactatggt gttggaaaat ggaaacgaat
     8641 ggatcgtgtc ttgatcgact gtctgatctt atcatttgtt gcaatgatga tcttgggaaa
     8701 cagcgcttat ttctttggtc ctaaactctt gacgatctat acaagtaatt caaaagtaat
     8761 tcaatgtgga atggaaatct tgttatatac aacggttacg tatttcctct gtggatttat
     8821 ggatctgttt cctggagcac ttcgtggaat ggggcgttct ggtgtgccaa tgatcctttc
     8881 gatcattggg acagtaggaa caagaatcgt atggattttc tggatcttcc cgaatcatag
     8941 atctttggat attttattta tatcatatcc agcatcctgg atcataacaa tcgtattaca
     9001 ggtgatctgt tattactttg tgagaaaaca attatatgca aaaatgagag cagaagcata
     9061 ataacaaaca ggcagtacat taaccagaac tggaagaatg tactgccatt ttttgttgga
     9121 atcctgtata tcggaattct atttttcctt tttatgaata tgaacctcag gaattgctac
     9181 attatcatag agaatagaat gtttttcttc tttttcctgt tctatttcat caatatgttc
     9241 ctcaatctca tcaaaatctt ttgtatggat ctcgggtatt gcaacatttt cgcttttcat
     9301 tttatattct gatttctcac ggagcttatt tcggattgct tcgcgtcttt tttttctttt
     9361 tccaaacatt tttcatcgtc tccctctaga ttatagaaaa ttgataaaga acgtgatcac
     9421 cagactattt aagaagtctg caaataaact accgatcaaa ggtaccagaa ggtaagcttt
     9481 gacggatggg acataacgtt cacagattgc ctgcatattc gccatggcgt ttggcgtggc
     9541 acccattccg aaaccacata cgccagaaga taatacagcg gcatcatagt ctcttcccat
     9601 gatattaaat actacaaaat aagtaaataa gaacatcaaa atagtttgtc ctgcaagtaa
     9661 tgtgatcagt ggaagtgcaa ggtctgcaag ctgccataat tttaaagtga tcatggcgat
     9721 tcctaagaat aaagataggc tgataccacc aatatcattg atctcaccca tataaattgt
     9781 aaattgtcca gaatattcgc cgatgttacg cataaatgca gcagcaatca tggcaccgat
     9841 atagattggg aatgtcatac ctgtcatgga aagcaatttg gaaatgatcg ttccaatccc
     9901 catggcgatg atcagctgga acacggcagc tggatacata cttgtgtgac gttcatgctt
     9961 tttctcttct tcaattaaca ggctgttatc ttcaggaatt gcagtcttta aaagattttt
    10021 cttctcaatc agacgttttc caacgggacc gcccatgata cttccagcga tcaatccaaa
    10081 tgtagcagca gctgtacata aagtgctggc acctttaaca ccaaagtctt caagaacagg
    10141 tccaaatgca ccagcggtac catgaccacc gatcataggg atagaacctg tacagagtcc
    10201 aaccaaagga ctgatctgta gtgctttgga cagaccgact gcaagaaagt tctggcatat
    10261 gatcaacatg atcacaagga tcaggaaaat gatcaaagat tttccaccac tttttaagac
    10321 ttttaagttt gcctgaaatc caacagatgt aaagaagaat accatacaga cttcttttag
    10381 aatatcatca aaggaaaatt cggcaaatcc agttacataa catacacagg taaagattgc
    10441 aaagatcaca ccgccgatca caggtgcagg aatacaaaat cgttctaaga tcgcaatcct
    10501 gtgttttaaa aagtttccta acatcaaaac gactacggca accgcgatcg tctgatacat
    10561 atctagttgt attgtcattt atttacaccg cagcggttaa ttgcttcctt tctctgtatt
    10621 tactgtgatt ccatgttgtt tataataggc agcagcaccg tcatggaatg gaatcgtgat
    10681 accatcaacg gcagttgtct catcaagaga aatatctaca ggtagtgcat actgaatctg
    10741 ctgttgattc ttaaacagaa tctgtgtaat gtctttgaca gtatcttcgg aaagtttatc
    10801 acttgcaaga agaacagctt tgacaccaac agttttaatt tctttagtct gatttacata
    10861 tgtacctgca ggtattgtat attctgtgta aaatttataa gatttcttca gacgagaaac
    10921 ccctttttga tcaagactga ccagacgaat cttacactgt tttgacagct cattgatcac
    10981 agtcgtctga acacctgcgg tacagaaaaa tgcatcaata tcgccagatt ttaatttctt
    11041 ggcggcatct gtataattca gatttacagt atcaacaagt ctttcgttta atccataagc
    11101 atttaaaatc tgcaacgcat tctgttcagt tccagattct tcttcaccaa tgcagacctt
    11161 tttcccttct agatcatcca tagactgaat gtctgaatcc gcaggaacca caatctgaca
    11221 ggcttctgta taaagggaag caacagcacg atatccctga tatttcttat cattttcaaa
    11281 aataccagtg cggttataag catcattgat caaatccatt tgggcaacag ccatctgaat
    11341 gtaatcttta gataacagac gaagatttgc agcagaacct gcagttgttt ttacttcaat
    11401 gttatatttt ttactcttag atgagataag gttggcaaaa gtatcaccaa aaatctggta
    11461 agtaccaccg attccagcag aaccaaagcg gatttttcca gaattcatct tacacccagt
    11521 caagcatgta ctgatcataa ttgcgacagc acaaagtacc ataagtttct ttttcaaaat
    11581 atcacctctc atttttaaca tttccttgtc tttattttaa cattaaattg ttatttcgaa
    11641 tagttttagg gcaaaaaaaa ttattcatga ccttcgttat atagtttttg tatgttttca
    11701 ggaagtttat caggcaacat ggcatttata gcatcttcat tacc
//
LOCUS       DAOMUE010000030        11737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1290_length_11737_cov_2.606061, whole genome shotgun sequence.
ACCESSION   DAOMUE010000030 DAOMUE010000000
VERSION     DAOMUE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11737
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1290_length_11737_cov_2.606061"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1396)
                     /locus_tag="PLC71_02670"
     CDS             complement(104..1396)
                     /locus_tag="PLC71_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01582.1"
                     /translation="MRKCGKGTLLLMSAVMAASLFTGCGKGKSSQAQKNEVYATIKKR
                     DQSASAAQICAWLSYRAKNNRLFQNEGIELSYCSDNLAVFSDSVGSVIYDRTKKKVIA
                     AVDLDKIGCDHFYSEGDEENPGLETAIKVSKDQKWMIIYNQLQGKVNGNIYAYSLKRC
                     DNMKFAKITPSKQISEKDKLYQTIIKDHKYTQKESDSIPGKIGDKIRAMDVSSSKYAY
                     QWKDSKGIRKQSVLVVDKDGLKLYTLSGKENQPDIQSEMVDLKTSDKIKLNTQLPEYE
                     YTGKDLRIKAVFDETKKRSDEDKEEGLVTIPMLNIYKIVETKSGAEVYANFWSETYYR
                     YGSLLKNYSGGSYPGVMYLKKTKDGYRVIKTRYAKDGAFLEKSIWKLCKGYPDVAVRM
                     MKDSVTQNQRKKVLQKYVSQNKLKIKAYKEYGWQYVNL"
     gene            complement(1539..2801)
                     /locus_tag="PLC71_02675"
     CDS             complement(1539..2801)
                     /locus_tag="PLC71_02675"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRM01583.1"
                     /translation="MILIKNARVMDPESGFDQVTDILLDGKRIRQIGKVDDVSGIEQV
                     IDASGMIAAPGLIDVHVHFRDPGFTYKEDLQTGSAAAAAGGFTTVVCMANTKPVVDSV
                     DIYKEIEDRCEQLPIKVWQAAAVSKGFEGKELTDMDALYEAGVRGFTDDGIPLMDEKL
                     VEEAMKKAKELDVPISLHEEDPAYIKQPGVNQGKVSEQLNYGGASYLAEAVMVKRDCE
                     LAVKTGAKVDIQHISSGVAVDYVKEAKEKGANVYAEASPHHFTLTEEAVLKYGTLARM
                     NPPLRTEEDRQRIIKGLQEGTIEIIATDHAPHSKEEKDKPLDQAPSGITGIETSLALG
                     VTELVEKGYLSMMQLLEKMTINPAKLYNMEQGRLQEGKPADVVLFDPEEEWEVKEYKS
                     KATNSPFTGWKLKGKVKYTICDGKIIEL"
     gene            complement(2825..3271)
                     /locus_tag="PLC71_02680"
     CDS             complement(2825..3271)
                     /locus_tag="PLC71_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01584.1"
                     /translation="MRKIIFNKKFLAIVGICLSVAGGFAIKQKITTKASDHSFEVNGM
                     NVSIQQCEGKSEEIMEEDLDETISNEVMALEEKGHNYEIGDTIETEEVAFVPMTKEID
                     DETAYNAFGTITSKSGNNYVIVVKSEKELTQDNLETVAEAVKEQVK"
     gene            complement(3344..>4885)
                     /gene="ppk1"
                     /locus_tag="PLC71_02685"
     CDS             complement(3344..>4885)
                     /gene="ppk1"
                     /locus_tag="PLC71_02685"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRM01585.1"
                     /translation="VERMIFLSGEGRRYVLLEDIILYFCDELFENYTVEEKAVLCITR
                     SADINPDDEIYESTDDYRTHMKKIIKMRARLKPVRLEIEGNRHKEIKKYLSERLNISE
                     QDIFKSQAPLDLKYVYKLTDKVSKEERKNGCYRPFVPALNRDFIPGVSVTKQILEEDK
                     LLSFPFDSMDTFIELLKESAKDKETLSIKITIYRLARQARIVKYLCEAAENGKEVLVL
                     MELRARFDEENNINYSEILEEAGCKVMYGMEDYKVHSKVCLITKKNSRGIYYITQIGT
                     GNYNESTSKLYTDLSLMTASEEIGHDASVFFHNMATFNLQGTYEHLLVAPSSLQDGII
                     KRIEREKMKAESFQPCGIFMKMNSLTDRKIIDELSKASNAGVPIFLLIRGICCIRPGI
                     PGKTENIRVERDDTEIYISSADMMTRNTRRRVEVAAPIYDPRLKQRILKMINVMKQDN
                     IQAQVLLSNGEYTTKKKREDNMNSQIHFLNEAKRLAPKEKTQKQNFFYQVKGIFFGKK
                     KLRKK"
     assembly_gap    4886..4916
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4917..5457)
                     /locus_tag="PLC71_02690"
     CDS             complement(<4917..5457)
                     /locus_tag="PLC71_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRM01586.1"
                     /translation="MMNKNYDFSYTQDRELSWLKFNERVLREADKKNVPIFERLKFLE
                     IFTNNLDEFFMVRVGSIHEMSLIHDNHRDIRSDLTPEEQLDEIAKHVRPLYELRDKIF
                     AKVSRELADKKIKRCQIEELEKEEKKKLKTYYEAMILPVLSPIVIGKQHPFPHIPNKV
                     LQIGLILKKKEKISFGIIGL"
     gene            complement(5536..>6027)
                     /locus_tag="PLC71_02695"
     CDS             complement(5536..>6027)
                     /locus_tag="PLC71_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRM01587.1"
                     /translation="YDLTQHQNTSGEDLTYFDDVNKERYIPYVIEPSLGADRVTLAFL
                     CSAYDEEELEGGDTRTVLHFHPALAPVKIGILPLSKKLSDGAMKVYEELAKEYNCEFD
                     ERGNIGKRYRRQDEIGTPYCVTFDFDSLEDGAVTVRDRDTMEQERVKIEDLKAYFAEK
                     FAY"
     assembly_gap    6028..6037
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6038..6924)
                     /locus_tag="PLC71_02700"
     CDS             complement(<6038..6924)
                     /locus_tag="PLC71_02700"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012739580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRM01588.1"
                     /translation="MKLSMEKIVNLAKVRGFVYSGSEIYGGLANTWDYGNLGVELKNN
                     VKKAWWKKFIQESPYNVGIDAAILMNPQTWVASGHLGNFSDPLMDCKECHERFRADKL
                     IEDYMQEHGMEIEGSVDAWSKEEMESFINEHDVVCPTCGKKNFTEIRQFNLMFKTFQG
                     VTEDAKNTVYLRPETAQGIFVNFKNVQRTSRKKIPFGIGQIGKSFRNEITPGNFTFRT
                     REFEQMELEFFCEPDKDLEWFEYWKNYCINWLQTLGIKEDEMRVRDHEKEELSHYSKA
                     TSDIEFMFPFGWGELWGIA"
     gene            7121..7453
                     /locus_tag="PLC71_02705"
     CDS             7121..7453
                     /locus_tag="PLC71_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM01589.1"
                     /translation="MYQIDYTNVGKRMKTLRKQAGYTQEQIADALDVTVAFISNIEND
                     RVKMNLRVLSYYAKLLNVSIDYLLRPESDENGEKDDVLNKEILRLLEKFSTDDKEKFV
                     KVLRILCK"
     gene            complement(7530..8252)
                     /gene="recO"
                     /locus_tag="PLC71_02710"
     CDS             complement(7530..8252)
                     /gene="recO"
                     /locus_tag="PLC71_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRM01590.1"
                     /translation="MSQEIKVTGMVLQAGNIGEYDKRIVLLTKERGRISAFARGAKKA
                     TSQYAAACQPFTFGEFSLYEGKNSFNLMWAGVENYFDTLKKDLDLIYYASYFCELASY
                     LTRENNDELEILKLLYQTLRALEKKTIDIELVRCIYEIKILALYGIGIEASRCVKCGS
                     REDLIWFDARESGVVCNKCEHGKVKVSPATLYTLQYILYSSIEKLYTFKVSADVLWEL
                     KKIIREYMRYHVNHEFKSLLFL"
     gene            complement(8255..9160)
                     /gene="era"
                     /locus_tag="PLC71_02715"
     CDS             complement(8255..9160)
                     /gene="era"
                     /locus_tag="PLC71_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRM01591.1"
                     /translation="MANEFKSGFVTFIGRPNVGKSTLMNRLIGQKIAITSSKPQTTRN
                     RIQTVYTDERGQIIFLDTPGINKAKNKLGNYMLQVAERTLNEVDVVLWLVEPSTFIGG
                     GEKYIIEQLSKIKTPIILVINKIDTVEEKDILEAIDTYKDVCEFAEIIPVSALKGKNT
                     DDVIDSIYKYLDEGPMYFDEDTITDQPERQICAELIREKALRLLSQEIPHGIAVVIDA
                     MKVRRHGHIVDIDATIVCERDSHKGIIIGKQGSMLKKIGTQARIEMENLLDMKVNLQL
                     WVKVKKDWRDSDMLLKNYGYDKKEV"
     gene            complement(9184..>11737)
                     /locus_tag="PLC71_02720"
     CDS             complement(9184..>11737)
                     /locus_tag="PLC71_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRM01592.1"
                     /translation="HVYLDAVFYPNIYKKPEILKQEGWHYDLLNEDAPLTYNGVVFNE
                     MKGVFSSPDQQLARIIQKSLLEDTPYGFESGGDPKAIPDLTYEMFLDFHKTYYHPSNS
                     YIYLYGDMDVDEYLTFIDEHYLKDFDEKQIDSSWEKQEPFTEVKRVEEYYPVGENDSE
                     EEKTYLSYNAVIGDSLDAKLYLGFEILNRVLFDAPGAPVKKALMDAQIGKDIQSSYDN
                     GIMQPVFSVIAQEARDDQEDEFVKILEKNLAKIAKEGIPRRNLLAAFNYYEFKYREAN
                     FGRFPKGLMYGLQMYDSWLYDDEKPFIHIKTNEIFKQLREEIENGYFENLIKEYLIDN
                     NHKTIVVMKPKKGLQKIKDQEEADKLKAYKDSLSEEEVKKLVEETKQLKASQEEASTK
                     EELEKIPVIDIEDIRKDVKPLSNVESELGGVKVLWHQYFTNKIAYVKLAFDMSHVPMD
                     LVPYASFLAEILTIVDTTHYSYQELGNEISIETGGISATMDVMPTDVHEFLPMFILKT
                     KCFYSNIEKAFDLLKEVAFESKLDNKKRLKEIIGQIYTNLKITLTETGHKSAANRAMS
                     YFSEYAAYREAIQGITMYETVKKWYEDFDEEYDNIVNGLKEAAKMIFEKQNMTISYTG
                     KEEAPEFMKAEVESFIEGLYEDQKQGEKVKVTCTKSNEGFATAGGVQYVACAGNFKDA
                     GLEYTGALKVLQMIFSYEYLWIQIRVKGGAYGCMCSFSDQGDSMFVTYRDPNLSESYK
                     VYDEAADYVADFDADDRDMKKYIIGTIGSMDMPMEAVDMGARSFHAYFLGKTEADLQK
                     VRDQVLSCTQEDIRALAPIVKAVVEAGNRCCIGNEEKIDQNKEYFDEVKHVL"
BASE COUNT         3072 a         2616 c         1767 g         4241 t
ORIGIN      
        1 tcctttttgc atcctgtcat catagatgga atcatactaa tacacaagat acttgcgatc
       61 gtttttttca atgtaagttt catattcttt acttactcct tctttataaa tttacatact
      121 gccatccata ctctttgtaa gctttgatct ttaatttatt ctgactaaca tatttttgta
      181 agactttctt tctctgattt tgtgtgacgg aatctttcat catccttact gcaacatcag
      241 gatatccttt acataacttc cagatcgatt tctccagaaa tgctccatct tttgcgtatc
      301 ttgtcttgat tactcgatag ccgtcttttg ttttcttcag atacatgacc cctggataac
      361 ttccaccaga gtagttcttt aacagacttc cataacgata atatgtttca ctccagaaat
      421 tcgcataaac ttctgctccg gattttgttt caacgatctt ataaatattc agcattggga
      481 ttgtaacaag tccttcttct ttatcttcgt cagatctctt ttttgtctca tcaaagactg
      541 ccttgatcct caagtctttc cctgtatatt cgtattctgg aagttgggta ttaagtttta
      601 ttttgtcgga tgttttaaga tctaccattt cactttgtat gtctggctgg ttttcttttc
      661 ctgataaagt atatagtttt aggccgtctt tgtctaccac taacaccgat tgttttctga
      721 ttcctttgga atctttccac tgatatgcat acttacttga tgagacatcc attgcacgga
      781 tcttatctcc gatctttcca ggtatgctgt ccgattcttt ttgtgtatat ttatgatctt
      841 tgatgatcgt ctgatacaat ttgtcttttt cactgatctg tttggatggc gtgatttttg
      901 caaatttcat gttatcacac cgtttgagcg aatatgcata aatatttccg tttacttttc
      961 cttgtaattg attgtaaatg atcatccatt tctgatcttt tgacaccttt atagccgtct
     1021 caagtcctgg attttcttca tctccttcac tgtagaaatg atcacatccg attttgtcaa
     1081 gatctaccgc agcaatgact ttcttctttg ttctatcata gatcacactt ccgacagagt
     1141 ctgaaaatac ggcaaggtta tctgagcaat acgacaattc tattccttca ttctgaaaca
     1201 acctattatt ttttgccctg taagaaagcc aggcacagat ctgtgctgca gatgctgact
     1261 gatctctttt tttgattgtt gcatagactt catttttctg tgcctgacta ctcttaccct
     1321 tcccacaccc tgtaaacaac gatgctgcca taacagcact catcaatagt aaagtacctt
     1381 ttccacactt tctcatcttt gttcctcctt attctcttat tgtctcctat cttattgttt
     1441 tcaactttat tatacaaaga aaaagcatca aaaaagaaaa caaaatctta agattcttct
     1501 acataattct taatgatttt gttttcttct tgtatacatt ataattctat gatcttgcca
     1561 tcacagatcg tatattttac ttttcctttt aatttccatc ctgtaaatgg agagttcgtt
     1621 gctttggatt tatattcctt gacttcccat tcttcctctg gatcaaacaa gacaacatcc
     1681 gcaggtttcc cttcctgcaa tcttccttgt tccatattat atagttttgc aggattgatc
     1741 gtcatcttct caagaagctg catcatagaa agatatcctt tctccacaag ttctgtgact
     1801 ccaagtgcca acgatgtctc gatccctgta attccacttg gtgcctgatc taatggtttg
     1861 tctttctctt ctttggagtg tggggcgtgg tctgtcgcaa tgatctcgat cgtaccttcc
     1921 tgtaatcctt tgatgatcct ctgacggtct tcttctgttc gcagtggcgg gttcattctt
     1981 gccagtgttc cgtattttaa tacagcctct tctgtcaacg tgaaatggtg tggagacgcc
     2041 tctgcgtaaa cattcgcacc tttttctttg gcttctttca cgtaatctac cgcaacacca
     2101 gaactgatat gctggatatc gactttcgct cctgttttga ctgctaattc acagtctctc
     2161 tttaccatca cggcttctgc caggtaagaa gctccaccat aatttaactg ttcggagact
     2221 tttccctggt tcactcctgg ctgtttaata tatgcaggat cttcttcatg aagactgatc
     2281 ggtacatcta attctttggc tttcttcatg gcttcttcta cgagcttctc atccatcagt
     2341 ggaattccat cgtctgtaaa tccacgaacc cctgcttcat agagtgcatc catgtctgtt
     2401 aattcttttc cttcaaatcc tttggacaca gctgctgcct gccatacttt gatcggaagt
     2461 tgttcacatc tgtcttcgat ctctttgtaa atatccacag aatccacgac aggctttgta
     2521 tttgccatac atactaccgt tgtaaatcca ccagctgctg ccgcagcaga acctgtctgt
     2581 aaatcttcct tgtatgtaaa tcccggatca cgaaaatgta catgtacatc gatcaatcca
     2641 ggggctgcga tcatcccgga agcatcaatg acctgttcaa tgccagagac atcatctacc
     2701 ttaccgatct gtctgatcct cttcccgtct aataaaatat ctgttacctg atcgaatccg
     2761 ctttctggat ccatcactct tgcattctta attaaaatca tctctgcctc cacgatcata
     2821 tgttttattt tacctgttct ttcactgctt cagcgacagt ttctaaatta tcttgtgtca
     2881 gttctttttc tgatttcacg acaataacat agttatttcc gctctttgat gtgatcgtcc
     2941 caaatgcatt gtatgctgtc tcatcatcaa tttcttttgt cattggtaca aacgctactt
     3001 cttctgtctc gatcgtatct ccgatctcgt aattatgtcc tttttcttcc agtgccatta
     3061 cctcattaga gattgtttca tctagatctt cttccatgat ctcctcagat tttccttcac
     3121 actgctgaat gctgacattc attccattga cttcaaaact gtgatcagag gctttggtcg
     3181 tgatcttctg tttgatcgca aatcctcctg ctacagataa gcagattcct acaatggcta
     3241 aaaacttttt gttaaaaata atttttctca tggttctatc cctccttgaa tgctggcatt
     3301 ttagtcagat atagtatacc ctattttata gataaattgc gagctatttt tttcttaatt
     3361 ttttctttcc aaaaaatatt ccctttacct gatagaaaaa attttgtttt tgtgtcttct
     3421 cttttggagc aagtcttttt gcttcattta aaaagtggat ctgtgaattc atattatctt
     3481 cacgtttttt cttagttgta tattctccgt tggaaagcag tacctgtgcc tgaatgttat
     3541 cctgcttcat cacattgatc atcttcagga ttctttgttt taaccttgga tcatagatcg
     3601 gtgctgcgac ttcaactctt cttcttgtat tccttgtcat catatccgca gatgaaatat
     3661 aaatctctgt atcatctctt tctacacgga tattctccgt cttccctggg attccaggac
     3721 gaatacagca gattccacga atcagcagaa atatcggaac tcctgcattt gacgccttcg
     3781 ataactcatc gatgatctta cgatctgtta aagagttcat tttcataaag atcccacatg
     3841 gctgaaagct ttctgctttc attttttcac gttcgatcct ttttatgatc ccatcctgaa
     3901 gacttgatgg tgccacaaga agatgttcat acgttccttg aagattaaat gttgccatat
     3961 tgtgaaagaa aacagatgca tcatgtccaa tttcttcgga tgctgtcata aggcttaaat
     4021 ctgtatataa tttcgatgta ctttcattat aatttcctgt tccgatctgt gtgatgtaat
     4081 aaattccacg actgtttttc ttcgtgatca gacaaacttt agaatgaact ttgtaatctt
     4141 ccattccata cataacctta caaccagctt cttctaagat ttcagaataa ttgatgttat
     4201 tttcttcatc aaatcttgcc ctaagttcca tcaatacaag aacttctttt ccattttccg
     4261 ctgcctcaca taaatatttg acgatccttg cctgccttgc caaacgataa atggtgatct
     4321 tgatagaaag agtttcttta tcctttgcac tttctttcaa taattcaata aatgtatcca
     4381 tgctgtcaaa cggaaaagat aataatttat cttcctcaag aatctgtttt gtcacactga
     4441 ctcctggaat aaaatctctg tttaacgctg gtacaaatgg acgatagcat ccattttttc
     4501 tctcttcttt tgacacttta tctgtcagct tgtaaacata ttttaaatcc aacggcgcct
     4561 ggcttttaaa gatgtcctgt tctgaaatat ttaaacgttc tgagaggtat tttttaatct
     4621 ctttatgacg attgccctca atttctaagc gaactggttt taatctggcc ctcatcttaa
     4681 tgatcttctt catatgtgta cgataatcat ccgttgattc ataaatctca tcatctggat
     4741 tgatatcagc acttcttgta atacataaaa ctgccttttc ttctaccgta taattctcaa
     4801 ataattcatc gcagaaatac aaaatgatgt cttctaaaag tacatatctt cttccttccc
     4861 ctgatagaaa gatcatacgc tccacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaag
     4921 tccaatgatc ccaaaagaga tcttttcttt cttctttaag atcaatccga tctgcaatac
     4981 tttattcgga atatgaggaa atggatgctg tttcccgatc acgatcggag aaagtactgg
     5041 cagtatcatc gcctcataat atgtctttaa ttttttcttt tcctcttttt ctaattcctc
     5101 gatctgacag cgtttgatct tcttgtctgc tagttctctg gatacttttg caaagatctt
     5161 atctcttaat tcatacagtg gtctgacatg ttttgcgatc tcatctaact gttcttctgg
     5221 ggtcagatcc gaacggatat ctctgtgatt atcatggatc agactcattt catgaatgct
     5281 gcccactctg accataaaaa attcatccag attgttcgta aatatctcta aaaatttcag
     5341 acgttcaaaa ataggaacgt tctttttatc tgcttctctt aaaacacgct cattaaattt
     5401 caaccaggat aattccctgt cctgtgtgta agaaaaatca tagtttttgt tcatcatcct
     5461 gccctccatc attcattcta tatcctatga aaaaatccgg agagatattg atattccccc
     5521 ggattctcat tcttattaat atgcaaattt ttctgcaaaa tatgctttca gatcttcaat
     5581 cttaacacgt tcctgttcca ttgtgtcacg gtcacgaact gtaacagctc catcttctaa
     5641 agaatcgaaa tcgaatgtta cacagtaagg tgttccaatc tcatcctgtc ttctataacg
     5701 cttaccaata ttaccacgtt cgtcgaattc acagttatat tctttcgcta attcttcata
     5761 aaccttcata gcaccatcgc ttaacttctt agataatggt aagataccga tcttaacagg
     5821 tgctaatgct ggatggaaat gaagaactgt tcttgtatct ccgccttcta attcttcttc
     5881 atcatatgca gagcataaga atgcaagtgt tacacggtct gctccaagag aaggttcgat
     5941 cacatatggg atgtaacgtt ctttgttcac atcatcaaaa tatgttaaat cttcaccaga
     6001 tgtattctga tgctgagtta agtcatannn nnnnnnntct gcgatacccc ataattcacc
     6061 ccatccgaat gggaacataa attcaatgtc ggatgttgct ttagaatagt gagataactc
     6121 ttctttctcg tggtcacgca ctctcatctc atcttctttg attccaagtg tctgtaacca
     6181 gttgatgcag taattcttcc aatattcaaa ccattctaaa tctttgtctg gttcacagaa
     6241 gaattctaat tccatctgtt cgaactcacg agtacggaat gtgaagtttc ctggtgtgat
     6301 ctcgttacgg aaagatttac cgatctgtcc gattccaaat gggatcttct ttcttgatgt
     6361 tctctgaaca ttcttgaagt ttacaaaaat tccctgtgct gtctcaggac gtaagtaaac
     6421 tgtattcttc gcatcttctg taacaccctg gaatgtcttg aacatcaggt tgaactgacg
     6481 gatctctgta aagttcttct ttccacaagt tgggcataca acatcatgct cattgatgaa
     6541 gctttccatc tcttctttgg accatgcatc cacagatcct tcgatctcca ttccatgttc
     6601 ctgcatataa tcttcgatca gtttatctgc acggaaacgt tcatgacatt ctttacaatc
     6661 cattaatgga tctgagaagt taccgagatg tcctgatgca acccatgtct gagggttcat
     6721 taagattgca gcatcaattc ctacattata tggactttcc tgaatgaact tcttccacca
     6781 agctttcttt acattgttct taagttctac accaaggttt ccatagtccc atgtattagc
     6841 aagtcctcca taaatttctg aaccgctgta tacaaatcca cgtaccttgg caaggttaac
     6901 gattttctcc attgataatt tcataatagc ctttacctct tcaaattttt gtattattat
     6961 aatattgtat cttgtaataa gtaatttttc aaggtatatt tcaaaagctt cacacttgaa
     7021 aatatatcaa atatactgaa tgaatattac tttttatatt tttttatatt ataatactta
     7081 atgttattat atgagatatg aagaaaggga atttataacc atgtatcaaa ttgactatac
     7141 caatgttggt aaaagaatga aaactctgcg taagcaggca gggtacactc aagaacagat
     7201 cgctgatgca ctcgatgtaa cagttgcatt catcagcaac attgaaaatg accgcgtaaa
     7261 gatgaacctt cgtgtactat cttattacgc aaaacttctg aatgtatcta ttgattatct
     7321 tcttcgtcca gaatcagatg agaatggtga aaaagatgat gtactgaaca aagaaattct
     7381 tcgtctattg gaaaaattct ctacagacga caaagaaaaa tttgttaaag ttcttcgcat
     7441 tttatgcaaa tagattacat atctcagaca taaaaagagc cgtatgaccg gctctttttt
     7501 tattcactct ttttcgttaa aatctgatct tataaaaata acaaagattt aaattcatga
     7561 ttcacatgat aacgcatata ttcgcgaatg atctttttta attcccataa aacatccgca
     7621 ctgactttaa aggtataaag cttctcgatc gaactgtaaa gaatgtactg aagtgtatat
     7681 aatgttgcag gacttacttt cacttttcca tgttcacatt tgttacatac cactccactt
     7741 tctcttgcat caaaccagat cagatcttcc ctgctcccac attttacaca tctggatgct
     7801 tcgattccga ttccatataa tgcaaggatc ttaatctcat aaatacagcg gaccaattca
     7861 atatcaatcg tctttttttc taatgcacgc agtgtctgat agagtaattt taagatctct
     7921 aactcatcgt tattctccct tgtcaggtag gatgcaagtt cacaaaaata actcgcataa
     7981 taaatgagat ccaggtcttt tttcagagta tcaaaataat tctcaacccc tgcccacatc
     8041 agattaaaag aattttttcc ctcatataaa gaaaactctc caaacgtaaa tggctgacag
     8101 gctgccgcat actggctggt tgctttttta gcacctcttg caaatgccga aatccttcca
     8161 cgttcttttg taagaagtac gatccgctta tcatactccc caatattgcc agcctgtaag
     8221 accattcccg tcactttgat ctcttgtgac atctttatac ttctttctta tcatatccat
     8281 agttcttaag cagcatatcg ctgtctctcc agtctttctt gactttaacc cataactgaa
     8341 gatttacttt catatccaga agattttcca tctcgatcct tgcctgtgta ccgatcttct
     8401 taagcataga tccctgcttt ccgataatga tccctttatg agaatctctc tcgcatacga
     8461 tcgttgcatc gatatccacg atatgtccat gacgtctgac tttcatcgca tcaatgacaa
     8521 cagcgatccc atgcgggatc tcctgactta acagacgaag agctttctca cggatcaatt
     8581 ccgcacagat ctgtctctct ggctgatctg tgatcgtatc ttcatcaaag tacattggac
     8641 cttcatctaa atatttgtaa atactgtcga tgacatcatc cgtattcttg cctttcaatg
     8701 cagataccgg gatgatctct gcaaattcac atacatcttt gtatgtatcg atcgcttcta
     8761 agatgtcttt ttcttctact gtatcaatct tgttgatgac taaaatgatc ggtgttttga
     8821 tcttagataa ctgttcaatg atatattttt caccaccacc gatgaacgta cttggttcta
     8881 ctaaccaaag tacgacatcg acttcattta atgttcgttc tgcaacctgt aacatatagt
     8941 ttccaagttt atttttcgcc ttgttgatcc ctggtgtatc aaggaagatg atctgccctc
     9001 tttcatcggt gtacactgtc tggatacgat ttcttgttgt ctgcggtttg cttgatgtaa
     9061 tcgcgatctt ctgtccgatc agacggttca ttaatgttga ctttccaacg tttggtcgtc
     9121 cgataaatgt tacaaaaccg gatttaaatt catttgccat aaattgtcct ttccctgttt
     9181 tctctatagt acatgtttta cttcatcaaa atattcttta ttctgatcga tcttctcttc
     9241 atttccaata cagcagcggt ttcctgcttc tactactgct ttaacgatcg gtgctaacgc
     9301 tctgatatct tcctgtgtac aggataatac ctgatctctg accttctgaa gatcagcttc
     9361 tgtcttgcct aagaaatatg catgaaaact tcttgccccc atatcaacag cttccattgg
     9421 catatccatg cttccgatcg ttccaatgat atatttcttc atatcacgat catctgcatc
     9481 aaaatctgcc acataatctg ctgcctcgtc gtatacttta taagattcag aaaggtttgg
     9541 atcacggtat gtcacaaaca tagaatctcc ctgatctgag aaactgcaca tgcatccata
     9601 cgcaccacct ttgacacgga tctgaatcca cagatattca taggagaaga tcatctgaag
     9661 cacttttaac gctcctgtat actcaagtcc tgcatcctta aagtttccag cgcatgccac
     9721 atactgaaca ccacctgcag ttgcaaatcc ttcattgctc tttgtgcatg taactttgac
     9781 tttctcacct tgtttctggt cttcatataa tccttcgata aagctttcca cttctgcctt
     9841 catgaactca ggtgcttctt cttttcctgt ataactgatc gtcatatttt gtttttcaaa
     9901 gatcattttt gcagcttctt ttaaaccatt gacgatattg tcatattctt catcgaaatc
     9961 ttcataccat ttcttcacag tctcatacat cgtgatcccc tgaattgctt cacggtatgc
    10021 tgcatattct gagaaataag acattgcacg gtttgccgca ctcttgtgtc ctgtctctgt
    10081 caatgtgatc ttaaggtttg tataaatctg tccgatgatc tcttttagtc gtttcttatt
    10141 atctaatttg ctttcaaatg ccacttcttt taataaatca aatgcttttt cgatattaga
    10201 atagaagcat tttgtcttta agataaacat tggaagaaat tcatgcacat ccgttggcat
    10261 aacatccatt gttgcgctga tgccacctgt ctcaatgctg atctcgtttc ctaattcctg
    10321 ataactatag tgtgttgtat caacgatcgt taagatctct gctaaaaatg atgcatatgg
    10381 cacgagatcc attggcacat gactcatatc aaatgcgagc ttgacgtaag cgatcttgtt
    10441 tgtaaaatac tgatgccata atactttcac accaccaagt tcagattcca cattggataa
    10501 tggtttgaca tcttttctga tatcttcaat gtcgatcact ggaatcttct ctaattcttc
    10561 ttttgtagat gcttcttcct gtgatgcttt taactgtttc gtctcttcga ctaatttctt
    10621 tacttcttct tcggataaag aatctttgta agcctttaat ttatccgctt cttcctgatc
    10681 tttgatcttc tgtaagcctt tctttggctt catcaccacg atcgttttat ggttattatc
    10741 gatcagatat tctttgatca gattctcaaa atagccattc tcgatctctt ctcttaactg
    10801 tttgaagatc tcatttgtct tgatatggat aaatggtttc tcatcgtcat ataaccagct
    10861 gtcgtacatc tgaagtccat acatcaatcc ctttggaaaa cgtccaaagt ttgcttctct
    10921 gtatttgaat tcatagtaat taaatgctgc taaaagattt cttcttggaa ttccttcttt
    10981 tgcgatcttg gctaagttct tttctaaaat cttaacaaat tcatcttcct gatcatccct
    11041 tgcttcctgt gcgatcacag aaaatactgg ctgcatgatc ccattatcat aagaactttg
    11101 aatatctttt ccaatctgtg catccatcaa agctttcttc actggtgctc caggtgcatc
    11161 gaataatact ctgtttaaga tctcaaatcc taagtaaagt tttgcatcca agctgtctcc
    11221 gatcactgca ttgtaggata aataagtctt ttcttcttcg gagtcattct ccccgactgg
    11281 atagtattct tccactcgtt tcacttctgt aaatggttct tgtttctccc atgatgaatc
    11341 gatctgtttc tcatcgaaat cttttaaata atgttcatcg ataaatgtca gatattcatc
    11401 aacatccata tccccataca gataaatata gctgttggat ggatgataat aagtcttgtg
    11461 gaaatcaagg aacatctcat aagttaaatc tgggattgcc tttggatctc cgcctgattc
    11521 aaacccatat ggtgtgtctt ccaataaaga cttctgaatg atacgtgcaa gctgctggtc
    11581 tggagatgag aagactcctt tcatctcgtt aaacacaact ccattatacg tcagaggagc
    11641 atcttcgttt aacagatcat aatgccatcc ttcctgcttt aggatctctg gcttcttata
    11701 gatatttgga taaaatacag catccagata aacatgc
//
LOCUS       DAOMUE010000031        11466 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1342_length_11466_cov_2.837525, whole genome shotgun sequence.
ACCESSION   DAOMUE010000031 DAOMUE010000000
VERSION     DAOMUE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 11466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11466
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1342_length_11466_cov_2.837525"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..948)
                     /gene="ispD"
                     /locus_tag="PLC71_02725"
     CDS             complement(160..948)
                     /gene="ispD"
                     /locus_tag="PLC71_02725"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRM01593.1"
                     /translation="MGEKIAAIVLAAGEGKRMGSGIPKQYMLIKSRPLVYYALKAFEE
                     SAVDEVILVTGEDEIDYCKEYIVDRYHFNKVKKIVPGGWERYASVYFGLEAIEDADYV
                     LIHDGARPMISAELIQKCIYYVKKYRACVVGMPSKDTIKVVDEENYAIRTPERKTLWQ
                     VQTPQCFEFDLVKKAYEKMMENDDGKVTDDAMVVETYGNVPVKLFEGGYDNIKVTTPE
                     DVRLATNLVRVRKVFHKLHVLHDKLIYLQMKAYKKRFREKKQKK"
     gene            complement(953..1981)
                     /gene="tsaD"
                     /locus_tag="PLC71_02730"
     CDS             complement(953..1981)
                     /gene="tsaD"
                     /locus_tag="PLC71_02730"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRM01594.1"
                     /translation="MSDKIKILAIESSCDETAAAVVEDGRCVKSNIISSQIELHKLYG
                     GVVPEIASRKHVEKVNFVVREALKEAGETLDSIDAIAVTYGPGLVGALLVGVAEAKAL
                     AFAKKKPLVGVHHIEGHICANYIEHPDLEPPFLCLVVSGGHTHLVDVKDYGKYEIIGC
                     TRDDAAGEAFDKVARAIGLGYPGGPKIEALAKEGDKNAISFPRAKVNDSKMDFSFSGL
                     KSSVLNYLNKCEMKGEEVNRADVAASFQEAVVDVLSSHGVEAAKELGYKKLAIAGGVA
                     SNGTFRAKMIERCKEAGIEFYHPSPIFCTDNAAMIGVCGYYDYINGVRSGLDLNAVPG
                     LKIGTRQG"
     gene            complement(1991..2905)
                     /locus_tag="PLC71_02735"
     CDS             complement(1991..2905)
                     /locus_tag="PLC71_02735"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRM01595.1"
                     /translation="MLDVCLLGTGGMMPLPYRKLTACMTRFNGHSLLIDCGEGTQVGI
                     KERGWSVKPLDAICFTHFHADHISGLPGLLLTLGNAERTEPLLIIGPKGVERVVNSLR
                     VIAPELPFPIEFYELNEKEHVIEMDGYKIEAFYVKHNVPCYGYNMIIPRAGKFDPVKA
                     KENDIPMKYWNRLQKGETVTSEDGRVLTSDMILGSPRPGIKLTYCTDTRPVQTISEHA
                     KNADLFICEGMYGEEGKEKKAREYKHMTFYEAAHLAKEADVKQMWLTHYSPSLVRAEE
                     FMGKVKSIFPRAKAGKDGKTITLDFQEE"
     gene            complement(2916..3650)
                     /gene="rimI"
                     /locus_tag="PLC71_02740"
     CDS             complement(2916..3650)
                     /gene="rimI"
                     /locus_tag="PLC71_02740"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRM01596.1"
                     /translation="MDKSSITAREWHVRKALPEDLPMIAEIEKLSFHRPWSFDSIESF
                     YYRKTSDIYVWRDRNRISGYIIAEHVLDQAELHRIAMIPFIRKMGIGTLLFHEFRRRY
                     QEIGVDTIYLEVRESNEAAYQFYLKNGFEEYGRRAKYYKDPIEDAILMFCEISTDDQS
                     FPLDSSETPVYNDERKEENEMKCNVCGQLLNNKTDYIEVKKEWGYFSNKDTQIHEFKI
                     CERCYDRIVKQFEISPKVTEKSEILS"
     gene            complement(3637..4347)
                     /gene="tsaB"
                     /locus_tag="PLC71_02745"
     CDS             complement(3637..4347)
                     /gene="tsaB"
                     /locus_tag="PLC71_02745"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRM01597.1"
                     /translation="MKLLVLDSSGLVASVALIEDDQLIAEYTTGNKLTHSQTLLPMLD
                     EVIKRTSFEIEDIDAVAVAKGPGSFTGLRIGAATAKGLGLALDKPIIPVPTVDGLAYQ
                     LFGTSMIICPMMDARRKQVYTGFYRFEGSEMKVLKEQCAQSVEDTLIQLREYNEPVVF
                     LGDGVPVYKEEILEKMGDLAIFAPAHANRQRAAAVGTLAQVYFAQGIYESADEFVPEY
                     LRKSQAEREREERLHGQK"
     gene            complement(4348..4773)
                     /gene="tsaE"
                     /locus_tag="PLC71_02750"
     CDS             complement(4348..4773)
                     /gene="tsaE"
                     /locus_tag="PLC71_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRM01598.1"
                     /translation="MIVQSFCAEDTYEIGKKIGQEAQPGQVICLYGDLGVGKTVFTKG
                     LADGLGITEPIQSPTFTIVREYEEGRLPLYHFDVYRIGDIEEMDEIGYEDYVYGEGVC
                     LIEWANLIEEILPDHYQKITIRKDLEKGFDYREIEMEEQ"
     gene            complement(4777..5412)
                     /locus_tag="PLC71_02755"
     CDS             complement(4777..5412)
                     /locus_tag="PLC71_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HRM01599.1"
                     /translation="MKFHTQLKAKDIFKFSLAYTYSGVQAILTIFMLGVGAYMIVHGI
                     GQPEGQTNIIFGVVVIALFVVINPLMIYVKAKKQAIENPVYKNPTYYTLRDDGIFVEL
                     GEESATIEWNRVYKITHFMGLTLLYTGKQQAFVFPDYELGDDREKMLDYMKEHIKEAR
                     KKAVQTQTDGGDISKYSKASAETEQEETEVETETQVEASGSEVQEKSEGEE"
     gene            5535..6077
                     /locus_tag="PLC71_02760"
     CDS             5535..6077
                     /locus_tag="PLC71_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HRM01600.1"
                     /translation="MKATGIIRRIDDLGRIVIPKEIRKTMHIRESDPLEIFTERDGDI
                     ILKKYSPIGELGNSAKLYAESIAAQIPHTICICDQDCVIAAAGPNAKKLIGKLLDEKA
                     EEALIDRKHILMDREKEDFFPLILDFPEVFRYVCLSTIIAESEAVGFVCILSIEDELD
                     DQEFKIAKIASDFLGKQLEA"
     gene            complement(6148..7077)
                     /locus_tag="PLC71_02765"
     CDS             complement(6148..7077)
                     /locus_tag="PLC71_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HRM01601.1"
                     /translation="MEIFKMTGKVFDIRRFSTHDGDGIRTTVFLKGCPLKCVWCQNPE
                     GISIRKRPIYFENRCMHCKTCIAKSKNQGVTMENDQIHIHPNRNENWNTIIDWCPTGA
                     IAMDSREMSVEMVMEEIRKDKSFYRYGNGGVTISGGEPLLQWKFTKELLKACKKEGIH
                     TAIETSLYADQEVIKEILPYLDRIFADFKLATEKDHMHYTGVSNQKIKDNIRYLLETS
                     NRDKVIIRTPMIPGMTATKDNIKGIAKYLNGIYQYVSYEILNYNPLAEAKYHLVDREY
                     CFEENPKLYTKEQMQEFKSWAVEGGLENIIIES"
     gene            complement(7064..9418)
                     /locus_tag="PLC71_02770"
     CDS             complement(7064..9418)
                     /locus_tag="PLC71_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HRM01602.1"
                     /translation="MEGLYQTQRIKDLKDKMLSEPRYASIEQALIITKTYQENEDDPK
                     IIQRAKSLKAALEKMEIRVEPEELIVGNRTAGVRYGVVFPESGSTWVDKEFETLPTRP
                     QDRFNVHQEDIETFRKVIKPYWEGKSLEDVLNQRYGKEINEIAKVVKINQKDHAQGHI
                     CPDCVKWLKMGPQGLLDQAEEKLKICKEGQKDFYESVKIVMEGAKHFMVRYHDLIMDM
                     AENESDETRKHTMIKVAKNCKNISERPVQTFHEAVQSTWFLFVILQMESNASSFSPGR
                     LDRHLEAYYEKDIKEGILDKQQALEIIECLWLKFNEIVYMRNSHGAKYFAGFPIGFNI
                     AIGGQDENGNDTYNDLSFLFLEAQKHLGLPQPNLSVRLHEGTSDELLKESVRVVAKGS
                     GMPQFFNDKAVIPSIQELGIEEKDARDYAIVGCVELTTQGNNLGWSDSAMFNLNKALE
                     VALTGGICLLTGEKIAPDYGNLETYETFKELEAAFKKQIDYFMDKMLLACEEVEKAHI
                     DLLPSPFLSASIENCMENGMDVTAGGAKYNLSGIQMIQVANLADSLVAIKQLVYDEKK
                     CTQKEMLDALKNNFEGYEILRAMCVNKVPKYGNDIDEVDKQGTKWADYFKNRLRTFKN
                     YRKGPYHTGMYTVSAHVPMGENVGATPDGRYAKEPLADGGMSPVYGRDIKGPTAVLKS
                     VSKLDKTLTTNGGLLNMKFLPEFFKTETGIDKFANFLRTFVDLEIPHIQFNVVRKEDL
                     LAAKKNPEQYRGLTVRVAGYTAYFTELADELQNEIIARTSYGDI"
     gene            complement(9446..>10220)
                     /locus_tag="PLC71_02775"
     CDS             complement(9446..>10220)
                     /locus_tag="PLC71_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRM01603.1"
                     /translation="KMFVEERHQKILHLLQENEKVKVKELSKRFEVTEDCIRKDLASM
                     EAKDLLKRTYGGAVLPDTTHPGHTNFVSTRKDKNIKEKQQIAKKAVKLIRPGDMIFLD
                     ISTTNIELAKEIQKQELEITVISCMMDIAQIFSQTKKVKFILLGGEFNRAQNGFLGEL
                     TIQMMKKFRFDACFMGVVGADVENDEVMTYVPEDGIMKSHAMKRSRRRYLVMENCKFD
                     FKANYVYTTFEDVDGILCEEKPSKKISEKLKEYQVEVIE"
     assembly_gap    10221..10230
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10375..>10691)
                     /gene="uvrA"
                     /locus_tag="PLC71_02780"
     CDS             complement(10375..>10691)
                     /gene="uvrA"
                     /locus_tag="PLC71_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391723.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HRM01604.1"
                     /translation="KLAAELSKRSTGKTIYILDEPTTGLHFADVHKLVDILARLTEGG
                     NTVIVIEHNLEVIKTADYIIDMGPEGGSGGGTVVATGTPEEICEVEKSYTGQYLKGIL
                     NR"
     assembly_gap    10692..10701
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10702..11466)
                     /gene="uvrA"
                     /locus_tag="PLC71_02785"
                     /pseudo
     CDS             complement(10702..11466)
                     /gene="uvrA"
                     /locus_tag="PLC71_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391723.1"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; frameshifted; incomplete;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
BASE COUNT         2969 a         2625 c         1755 g         4097 t
ORIGIN      
        1 cgcccttgcg gacaaacggt tttcaagacc gcctcgttat gaccacttcg atacctctcc
       61 ataacgtatt ctgttgttgt cgttttgtgt gtctcacgcg acgacttgtt tataatacca
      121 aaccagaata catatgtcaa cacttttttt aaagtttttt tatttttttt gttttttctc
      181 tctaaatcgc tttttatagg ccttcatctg tagatatatc aacttatcat gtaacacatg
      241 aagcttgtga aaaactttcc ttacacgtac aagattcgtt gcaagtctaa catcctctgg
      301 tgtcgtgacc ttaatgttat cataaccacc ttcaaacagt tttaccggca cattcccata
      361 tgtttctact accattgcat catcggttac ttttccatca tcattttcca tcatcttttc
      421 atatgccttt tttacaaggt caaattcaaa acattgtggt gtctgaacct gccacaatgt
      481 ttttctctca ggtgtacgga tcgcgtaatt ctcctcatca acaactttaa tcgtatcttt
      541 cgatggcatt cccacgacac atgcacgata tttttttaca tagtatatgc atttttgtat
      601 taattctgca ctaatcatcg gacgtgctcc atcatggatc agtacataat ctgcatcctc
      661 aattgcttca agcccaaaat aaacggatgc ataacgttcc catccgcctg gcacgatctt
      721 tttcacctta ttaaaatgat aacggtctac aatatactct ttacagtaat ctatctcgtc
      781 ttctcctgta acaagaataa cctcatctac agcgctctcc tcaaaagcct tcagcgcata
      841 gtacaccaaa ggccttgatt tgatcagcat atattgtttt gggattccgc ttcccattct
      901 ttttccttct cctgctgcca aaacgattgc cgcaattttt tctcccatgt atttacccct
      961 gtctggtacc aatctttaat ccaggtaccg cattcagatc taaaccgctt cttacaccat
     1021 tgatatagtc atagtatcca caaaccccga tcattgctgc attatctgta cagaaaatag
     1081 gagatggatg ataaaattca attcctgctt ccttacatct ctcgatcatt tttgcacgaa
     1141 atgttccatt ggatgcaact ccacctgcga ttgcaagttt cttgtatcct aattctttcg
     1201 ccgcttctac tccatgagaa ctcaaaacat ctacaaccgc ttcctggaaa gaagccgcga
     1261 catccgcacg gtttacttct tcccctttca tctcacattt atttaaataa tttaatacag
     1321 aggattttaa cccgctaaag ctaaagtcca tcttggaatc atttactttg gctctaggga
     1381 atgaaattgc attcttatct ccctcttttg ctaaggcttc aatctttgga cctcctggat
     1441 atcccagtcc aatcgcacgt gctactttat cgaaggcttc tcctgctgca tcatctcttg
     1501 tacacccaat gatctcgtat tttccataat ccttgacatc aacaaggtgt gtatgccccc
     1561 ctgatacgac cagacataaa aatggaggtt ctaaatctgg atgttcaata taattagcgc
     1621 agatatgtcc ttcaatatga tgcactccta ccaatggttt cttctttgca aacgcgagtg
     1681 ctttcgcttc tgccacacca actaatagtg caccgactaa tcctggtcca taagtaaccg
     1741 cgatcgcatc aatactgtct aatgtttctc ctgcttcttt taatgcttct cttactacaa
     1801 agttcacttt ctctacatgt tttcttgagg caatctcagg gacaacaccg ccatatagct
     1861 tatgcagttc gatctgagat gaaatgatat tggattttac acatcttcca tcctctacaa
     1921 ctgctgccgc tgtctcatcg caggaagatt cgattgccag aatcttaatc ttatcgctca
     1981 tcgtattctc ctattcttcc tgaaaatcta aagttatcgt cttaccgtct tttccagctt
     2041 ttgctctagg gaaaatcgac ttcactttcc ccataaattc ttctgcccgg actaatgacg
     2101 gactataatg agttaaccac atctgtttta catctgcttc ttttgcaaga tgcgcagctt
     2161 cgtaaaatgt catgtgttta tattctcttg ctttcttttc ttttccttct tctccataca
     2221 ttccttcaca aataaataaa tctgcattct ttgcgtgttc agaaattgtc tggactggtc
     2281 ttgtatccgt acaatatgtc aatttaattc caggtctagg acttcctaag atcatatcac
     2341 ttgtaagtac acgaccatct tcacttgtca cagtttctcc cttttgaagc ctgttccagt
     2401 atttcatcgg gatgtcattt tcctttgcct ttacaggatc aaacttccca gctcttggga
     2461 tgatcatatt atagccataa caaggcacat tatgttttac atagaaagct tcaattttat
     2521 agccatccat ctcaataacg tgctctttct catttaattc ataaaattcg attggaaatg
     2581 gaagttctgg tgcgatcacg cgcagtgaat tcacaaccct ttctacgcct tttggtccta
     2641 tgatcaaaag aggttctgtt cgttctgcat ttccaagtgt taaaagaagt cctggaagtc
     2701 cactgatatg atctgcatga aaatgtgtaa aacagatcgc atctaatggt ttaacactcc
     2761 agcccctctc tttaattcct acctgtgttc cctccccgca gtcgatcaac agactatgcc
     2821 cgttgaatct tgtcatacat gcggtcaact ttctatatgg aagcggcatc attccaccgg
     2881 ttccaagtaa acatacatct aacataaatc accttttatg ataatatctc actcttctct
     2941 gttacctttg gagaaatctc aaattgtttt acgatacgat cgtaacatct ctcacaaatc
     3001 ttaaattcat gaatctgcgt atctttgttt gagaaatacc cccattcttt tttaacttct
     3061 atataatctg ttttattgtt taataactgt ccacatacat tacatttcat ctcattctcc
     3121 tccttacgct catcattata aacgggcgtt tctgaagaat ccagtggaaa tgattggtca
     3181 tctgttgaaa tttcacaaaa cattaagatc gcatcctcga ttggatcctt ataatacttt
     3241 gctctgcgtc catattcttc aaatccattc tttaaataaa actgatacgc tgcttcgttt
     3301 gattctctga cctccaaata gatcgtgtct acccctatct cctgatacct tcgtctgaac
     3361 tcatgaaaca acaatgtgcc aattcccatt ttccttataa atggaatcat tgcgatccta
     3421 tgtaattctg cctgatctaa cacatgctca gcgattatat atccactaat acgatttctg
     3481 tctctccata cataaatatc actagtttta cgataataga aagactcaat actatcgaaa
     3541 ctccatggtc tgtgaaaaga aagtttttct atctctgcga tcatcggaag atcttctgga
     3601 agtgctttcc tcacatgcca ttctctggct gttatactac ttttgtccat gaagcctctc
     3661 ttctctctct cgttcagcct gagattttct taaatattct ggcacaaatt cgtctgcact
     3721 ttcataaatt ccctgtgcaa aatatacttg tgctaacgtt ccgactgctg ctgctctctg
     3781 gcggtttgca tgtgctggag caaaaattgc cagatcaccc atcttctcta agatttcttc
     3841 tttatatact ggtacgccat ccccaaggaa tacaactggt tcattgtatt ccctcaactg
     3901 tatcaaagta tcttctacac tctgggcaca ttgttccttt aagactttca tttcactgcc
     3961 ttcaaatcgg taaaatcctg tatacacttg ttttcttctg gcatccatca ttggacaaat
     4021 gatcatggac gttccaaata actgatatgc aagcccatct acagttggca caggaatgat
     4081 cggtttatcc agtgccagcc ctaatccttt ggctgttgct gcaccaatgc gaagtcccgt
     4141 aaaagaacct ggtccttttg ctaccgcaac cgcatcaata tcttctatct caaaggatgt
     4201 tctcttgatt acttcatcta acatcggaag taacgtctga gaatgtgtta atttatttcc
     4261 agtcgtatac tctgcgatca actgatcatc ttcgatcaat gccacagaag ctaccagacc
     4321 agagctgtcc agtactaata atttcatcta ttgttcctcc atttcaatct ctcgatagtc
     4381 aaatcctttt tctaaatctt tacggatcgt aatcttttga taatgatctg gcagaatctc
     4441 ctcgatcaga tttgcccatt cgatcagaca gactccttct ccataaacat aatcctcata
     4501 tccaatctcg tccatctctt caatatcacc aatacgataa acatcaaaat gatatagtgg
     4561 aagccttccc tcttcatatt ctcttacgat cgtaaaagtc ggactctgaa ttggttctgt
     4621 gataccaagt ccatctgcca acccttttgt aaatacagtc ttaccaaccc caagatctcc
     4681 atataaacag atcacctgtc caggctgtgc ctcctgtccg atcttctttc cgatctcata
     4741 cgtatcttct gcacaaaaac tttgtacgat cattttctat tcttctcctt ctgatttctc
     4801 ttgaacttct gatcctgatg cttcaacctg agtttccgtt tctacttctg tctcttcctg
     4861 ctcagtttct gctgatgcct tggaatattt tgaaatatct cctccatctg tctgtgtctg
     4921 aacagccttc tttcttgctt ctttgatgtg ttctttcata taatctaaca tcttttctct
     4981 gtcatcacca agttcataat ctggaaatac aaatgcctgc tgttttcctg tatacaataa
     5041 tgtcagcccc ataaaatgtg tgatcttata aactctgttc cactcgatcg tcgcactttc
     5101 ttctcctagc tctacaaaaa taccatcatc tcgtaaagtg tagtacgttg gattcttgta
     5161 tactggattc tcaattgcct gtttctttgc ttttacatat atcatcagtg gattaatgac
     5221 aacaaataat gcaataacga ctacaccaaa aataatattc gtctgtcctt ctggctgacc
     5281 gattccgtgg acgatcatat atgctccgac tcccagcata aaaatcgtca aaatcgcctg
     5341 tactccggaa tacgtgtatg ctaatgaaaa tttaaaaata tctttcgctt ttaattgtgt
     5401 atgaaatttc atcttaattc tccttacctt ttctctgtta atgaaatatt ataccataat
     5461 ccgggtataa aaccaatgat tttacaaata ctacaagcat acacgaaaac caatgaatat
     5521 ggaggatctt aagaatgaaa gctacaggta ttatacgaag gattgatgac cttggccgga
     5581 tcgttatacc aaaagaaata cgaaaaacca tgcatatcag agaaagcgat cctttggaga
     5641 tatttacaga gagggacggc gatatcattt taaagaaata ttccccgatc ggtgaactag
     5701 gaaacagtgc gaagctatat gcagaatcga tcgctgctca gattccacat acgatctgta
     5761 tttgtgatca agactgtgtg atcgctgctg ccggaccaaa tgccaaaaaa ctaattggaa
     5821 aattattaga tgaaaaagct gaagaagctt tgatagatcg aaaacatata ttgatggacc
     5881 gtgaaaaaga agatttcttt cctctgatct tggacttccc agaggtattt cgctatgtgt
     5941 gtctttctac gatcattgca gaaagtgaag ccgttggatt tgtatgtatt ctttctattg
     6001 aagatgaatt agatgatcag gagtttaaaa ttgccaaaat cgcttctgat tttctaggaa
     6061 aacagctcga agcataacgt aaaaaagtga aatacagtcg cacaaaacgg ctgtacattt
     6121 cacttttttc ttcatgatac atatttatta tgattcaata ataatatttt ctaatccacc
     6181 ttcgactgcc cagcttttaa attcctgcat ctgttctttt gtgtacagtt tgggattttc
     6241 ttcaaaacaa tattctctat ctaccaagtg atattttgct tctgccagtg gattgtagtt
     6301 taagatttca taggaaacat actgatagat cccatttaaa tattttgcaa ttcccttgat
     6361 gttatctttt gttgcggtca ttcctggaat catcggtgtt cttatgatga ccttgtctct
     6421 atttgatgtc tctaataagt atctgatatt atccttgatc ttttgatttg aaaccccagt
     6481 gtaatgcata tgatctttct ctgttgctaa tttaaagtct gcaaaaatcc ggtctaaata
     6541 tggaaggatc tctttgatca cttcctgatc tgcatataag gatgtctcga tcgcggtatg
     6601 gattccttct tttttgcatg cttttaacag ctcttttgta aacttccact gtaataatgg
     6661 ttcccctcct gagatcgtaa ccccgccatt tccataacga tagaatgatt tatctttgcg
     6721 gatttcttcc ataaccattt ctacgctcat ctctctggaa tccatggcga ttgctcccgt
     6781 tggacaccag tcaatgatgg tattccagtt ttcatttctg tttggatgaa tgtgtatctg
     6841 gtcattttcc atcgtaacgc cctgattttt tgattttgca atacacgtct tacaatgcat
     6901 gcagcggttt tcaaaataaa tgggtctttt tcggatggaa attccttctg ggttctggca
     6961 ccagacacat tttaacggac atccttttaa aaatactgtg gtacggattc cgtctccatc
     7021 gtgcgttgaa aatctgcgaa tatcaaatac ttttcctgtc atcttaaata tctccataac
     7081 ttgttcttgc gatgatctca ttttgcagtt catcggcaag ttctgtaaag taggctgtgt
     7141 aacccgcaac tctgacggtc aatccgcggt attgctctgg attcttcttt gctgcgagca
     7201 agtcttcttt tcttactacg ttaaactgga tatgtggaat ctcaagatct acaaatgttc
     7261 ttaagaaatt tgcaaactta tcaattcctg tctctgtctt gaaaaattca ggaaggaatt
     7321 tcatatttaa taatccgcca ttggttgtaa gtgtcttatc tagtttggat acggatttta
     7381 atacggctgt tggtcctttg atatctcttc cgtaaacagg tgacattcct ccatctgcta
     7441 atggttcctt cgcatatctt ccatctggag ttgcgcctac attttcaccc attggcacat
     7501 gggctgatac ggtatacatt cctgtatgat atggaccttt tcgatagttt ttaaatgttc
     7561 ttaatcgatt cttgaagtaa tctgcccatt ttgttccttg tttgtcgact tcatcaatgt
     7621 catttccgta ctttggaact ttattgacgc acattgctct taagatttca tatccttcaa
     7681 aattgttctt taatgcatca agcatttctt tttgtgtgca ctttttttca tcatacacca
     7741 gctgcttgat tgcgacaaga ctatccgcaa gatttgcaac ctgaatcatc tgaattccgg
     7801 ataaattgta ttttgcaccg cctgctgtga catccattcc attttccata cagttttcaa
     7861 tggatgctga taaaaatgga gatggcagaa gatcgatatg ggctttttct acttcttcac
     7921 atgcaagaag catcttatcc atgaaataat caatttgttt cttgaatgct gcttccaact
     7981 ctttaaatgt ctcgtacgtt tcaaggtttc cataatctgg tgcgatcttt tctccggtta
     8041 agagacagat tcctcctgtc aatgccactt ctaatgcttt atttaagtta aacattgcag
     8101 aatcactcca tccaaggtta tttccctgcg ttgttaattc cacgcatcca acgatcgcat
     8161 aatctcttgc atctttttct tcgattccta attcctgaat ggatggaatg actgccttgt
     8221 cattgaagaa ctgtggcatt ccgcttcctt tggctacgac tctcactgat tcttttaata
     8281 attcgtccga cgttccttca tgaagtctta cggaaaggtt tggctgtgga agtcctaaat
     8341 gtttctgagc ttctaagaac aggaatgata aatcattata tgtatcattt ccattctcat
     8401 cctgaccgcc gatcgcgata ttaaatccaa tcgggaatcc tgcaaaatat tttgctccgt
     8461 gagagtttct catatatacg atctcattaa atttcaacca taaacattcg atgatctcta
     8521 acgcctgctg tttatctaag attccttctt tgatatcttt ttcataataa gcttctaaat
     8581 gtctgtctaa tcttcctggt gaaaatgaag atgcattgga ttccatctgt aagattacaa
     8641 ataagaacca tgtagactgt actgcttcat gaaatgtctg tactggacgt tcagaaatat
     8701 tcttacagtt tttggctact ttgatcatgg tatgttttct tgtttcatct gattcatttt
     8761 ctgccatgtc catgatcagg tcatgataac ggaccataaa gtgtttcgct ccttccatga
     8821 cgatcttaac actttcatag aagtctttct gtccttcttt acatatcttt agtttttctt
     8881 ctgcttgatc aagtagtccc tgcggtccca tctttaacca tttgacacaa tctgggcaga
     8941 tatgtccttg tgcgtggtct ttctggttga tctttacaac tttggcgatc tcgttgatct
     9001 cttttccata tctctggttc aggacatctt ctaaggattt tccttcccag tatggtttga
     9061 tgaccttacg gaatgtctca atatcttctt gatgtacatt gaagcggtcc tgtggtcttg
     9121 ttgggagtgt ttcaaattct ttatctaccc atgtgctacc actctctgga aatacaactc
     9181 cataacgaac tccagctgta cgatttccaa cgatcagttc ttcaggttct acacggatct
     9241 ccatcttctc taaagctgct tttaatgatt ttgctctctg tatgatcttt ggatcatctt
     9301 cattctcctg atatgttttt gtaatgatca gtgcctgttc aattgatgcg tatcttggct
     9361 ctgataacat tttatctttt aaatctttga ttcgctgtgt ctgatataat ccttccatag
     9421 aatttgtcct ttctaattta attctttact caataacttc tacctgatat tcttttagtt
     9481 tttcactaat tttttttgac ggcttttctt cacataaaat accgtcaaca tcttcaaatg
     9541 ttgtatatac ataattagct ttaaagtcaa atttacagtt ttccattacc aaatatcgtc
     9601 ttctacttct tttcatcgca tgagatttca taattccatc ttctggtaca tatgtcatta
     9661 cttcatcatt ctctacatct gctcccacaa ctcccataaa gcaagcgtca aatctgaatt
     9721 tcttcatcat ctgaattgtc aattctccca gaaatccatt ttgagcacga ttaaattctc
     9781 cacctaacaa aataaatttt actttctttg tctgtgaaaa aatctgtgca atatccatca
     9841 tgcagcttat aactgtaatt tcaagttctt gtttttggat ttcttttgcc aattcaatgt
     9901 ttgttgttga aatatccaga aaaatcatat ctccagggcg aattagcttc actgcttttt
     9961 ttgcaatttg ctgtttttct tttatattct tatcttttct tgttgacaca aagtttgtat
    10021 gtccgggatg cgtagtatct ggaagtactg ctcccccata agttcttttt agaagatcct
    10081 ttgcttccat acttgctaaa tcttttcgaa tacaatcttc tgttacttca aatcgtttgc
    10141 ttagctcctt gacttttact ttttcatttt cctgaagcaa atgtaagatt ttctgatgtc
    10201 gttcttccac aaacatcttt nnnnnnnnnn gttatttatt attttatatt gtttttattt
    10261 attgtcaagt caaaacaaga aaagacagcc attcaaaatg actgtctcag actgtagaca
    10321 aatatttgtt tttcttttat ttctgtcaca tacacacaag cctatccaca gattttatcg
    10381 attaagtatc cctttcaaat actgcccagt ataagatttc tcaacctcgc agatctcttc
    10441 tggtgtccct gttgcaacaa ctgttccacc gcctgatcca ccttctggtc ccatatcgat
    10501 aatataatcc gcagtcttga tcacttccag attatgctcg atcacaatca cagtattgcc
    10561 accttctgtc agtcttgcaa ggatatccac cagtttgtga acatctgcaa aatgaagtcc
    10621 tgtcgttggc tcatccagga tatagatggt ctttcctgta cttcgtttgc ttaattctgc
    10681 tgccagtttg annnnnnnnn ngtcctaatc gaatgtatcc taatccaaca tctcttaatg
    10741 tctccatctt acggctgatc gatggaatat tagagaagaa atcacatgct tcttccactg
    10801 tcatattcag aacatcataa atactctttc ccttgtattt aacttcaagt gtttctctat
    10861 tgtatcgttt tccatgacat acttcacaag ggacatacac atctggtaaa aagtgcatct
    10921 caatcttgat gatcccatct cctgcacagg cttcgcatcg cccgcctttc ttgttgaaac
    10981 tgaatcttcc tttattgtat ccttttgctt ttgcatcttt tgtcatcgca aacagatctc
    11041 tgatctgatc aaagactcct gtgtaagttg caggattgga tcttggtgtt cttccgatcg
    11101 gtgactggtc gatcgctatg atcttatcta actggtcaaa tccttcgatg tctttgtgta
    11161 gtcctggttt cgtcttagcc ctgtttagtt cttttgccag acgtttgtat aaaatctgat
    11221 tgactaaaga actctttcct gatccagaga ctcctgttac acaagtgaaa attccaagtg
    11281 ggatttctac atcaatattc tttaaattat tctcctgtgc cccgactact tttaggaatc
    11341 ctgatggttt tcttctgact tgtggtactg gaatcttgat ctttccgctt aaataagctc
    11401 ctgtgatgga tttcttattc ttcatgatct gttttgcagt tcccgttgca acaacttctc
    11461 caccat
//
LOCUS       DAOMUE010000032        11375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1357_length_11375_cov_3.276943, whole genome shotgun sequence.
ACCESSION   DAOMUE010000032 DAOMUE010000000
VERSION     DAOMUE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 11375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11375
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1357_length_11375_cov_3.276943"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLC71_02790"
     CDS             complement(<1..498)
                     /locus_tag="PLC71_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRM01605.1"
                     /translation="MSSNYTPWERVQISRSAERPTSLDYISTIFERYMSLHGDRHFGD
                     DKAIVGGLASIGGRPVTVIGQQKGRSTKENIKRNFGMPSPEGYRKSLRLMKQAEKFHR
                     PIILFVDTPGAFCGLEAEEGGVGEAIARNLFEMSNIKVPILSIMIGEGGSGGALAMAV
                     GNEVWS"
     gene            complement(488..1372)
                     /gene="accD"
                     /locus_tag="PLC71_02795"
     CDS             complement(488..1372)
                     /gene="accD"
                     /locus_tag="PLC71_02795"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRM01606.1"
                     /translation="MSLKDMFKKRTLSPQEIKQAERVRKAKDTLLEFQAPEGLFQKCN
                     CCGKPVYNEDLIENDYVCPVCGNYFRIRPKTRIAMVLDKDTFEEWDAKMDTSDPLNFP
                     GYKNKLERQRSVTNLDEAVITGKGKILGKDVVIAVMGADFMMGSMGEVVGEKITRAVE
                     RATQMRLPIIIFTCSGGARMQEGIVSLMQMAKTSAALKRHDEAGLLYITVLTDPTTGG
                     VTASFAMLGDVILAEPGALIGFAGPRVIEQTIGQKLPEGFQRAEFLLEHGFVDKIVER
                     KKLRKVLVTIIRSCEYEQ"
     gene            complement(1393..>2127)
                     /locus_tag="PLC71_02800"
     CDS             complement(1393..>2127)
                     /locus_tag="PLC71_02800"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203701.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRM01607.1"
                     /translation="QILADKYGNTIHLGERDCSIQRRHQKLIEESPSPAISDEQRKKM
                     GEIAVKAAKAAEYYSAGTIEFLRDKHGDFYFIEMNTRIQVEHPVTEWVTGIDLIREQI
                     RIAAGKHLTYTQEDVHVHGHAIEVRINAEDTEHDFRPSPGTVTNVHFPGGKGVRIDSA
                     LFAGYQIPPYYDSMIAKLIVYDKNRKLALRKLRNALGELVLEGVKTNIDYQYEIINDE
                     DFIEGNVDTGFIERFQKKHQSENEAE"
     assembly_gap    2128..2137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2138..2809)
                     /locus_tag="PLC71_02805"
     CDS             complement(<2138..2809)
                     /locus_tag="PLC71_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HRM01608.1"
                     /translation="MFKKILIANRGEIAVRIIRACREMDIMSVAICSEADKDALHAQL
                     ADQTICIGPAPAKDSYLDMERVLGAAMAVGADAIHPGFGFLSENSKFVRMCEKCNITF
                     IGPSAEIIDRMGNKSAARKTMMDAGVPVVPGTKDAVYEAEAGQKIADDMGYPVMIKAS
                     AGGGGKGMRVCYDSEDFVHTFETAKLEAENAFGDGTMYIEKYIEDPRHIEFQIFGDGR
                     GNAIHL"
     gene            complement(2825..3250)
                     /gene="fabZ"
                     /locus_tag="PLC71_02810"
     CDS             complement(2825..3250)
                     /gene="fabZ"
                     /locus_tag="PLC71_02810"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRM01609.1"
                     /translation="MVMDIKDIMNTIPHRSPFLLVDRIEEMEEGKRIVGKKCVTYNEP
                     FFAGHFPQEPVMPGVLIIEALAQTGAVCALSADEYKGKIAFLGGVNKAKFRGKVVPGD
                     VLTLEVEMIKVKGPVGVAQGTATVDGKKVASAEITFMIG"
     gene            complement(3270..3713)
                     /gene="accB"
                     /locus_tag="PLC71_02815"
     CDS             complement(3270..3713)
                     /gene="accB"
                     /locus_tag="PLC71_02815"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRM01610.1"
                     /translation="MEVKEMKELIAAVSSANVDEFEYNNEEFSIRIAKKKAKIATVDA
                     SVAGVPVQSAAIMPEQNEEQPEELLYGDEIKAPLVGTFYVAPSEGAEPFVSVGDKVKK
                     GQVIGIVEAMKLMNEVESEYDGTVAAILVENGEMVEYGQPLMVIQ"
     gene            complement(3731..4966)
                     /gene="fabF"
                     /locus_tag="PLC71_02820"
     CDS             complement(3731..4966)
                     /gene="fabF"
                     /locus_tag="PLC71_02820"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRM01611.1"
                     /translation="MKRVVVTGMGAITPIGNSVEEFWNGIKEQKIGFAPITYFDATEY
                     KAHLAAEVKGFNAKDYMSPKAARRMELFSQYAVAATKEAIEDAGLDLEKEDTTRIGVS
                     VGCGVGSLQMIETTTRTLDKKGPNRVKPLAVPMMISNMAAGNVSIAFGLRGKSINVVT
                     ACATGTQSIGEAYRSIQVGEADVMVAGGTEAAVSEVAVGGFAALTALSGSDDPKRASI
                     PFDKERDGFVIGEGSGIVVLESLEHAQARGADILAEVVGYGATSDAFHITSPCEDGEG
                     AARAMVFAMDEAGITPDKVDYINAHGTSTHANDLFETKAIKKALGEHAYDVKISSTKS
                     MIGHGLGAAGAIEFITCVKSIEEDYIHPTVGLKVPDEECDLDYTLEPVTDRKVNYVMS
                     NSLGFGGHNATLLVKKFED"
     gene            complement(4988..5728)
                     /gene="fabG"
                     /locus_tag="PLC71_02825"
     CDS             complement(4988..5728)
                     /gene="fabG"
                     /locus_tag="PLC71_02825"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008726615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRM01612.1"
                     /translation="MLSGKVAIVTGASRGIGRETALTLAGYGATVIVNYCGSKDKAEA
                     VVEEITAAGGTAKAYQGDVSDFDVAKEMITSVKKEFGSIDILVNNAGITKDNLIMKMS
                     EEEFDKVIEVNLKGAFNCMKHVSRIMLKQRSGHIINMSSVSGVMGNAGQVNYCASKAG
                     IIGMTKSLARELGSRGITVNAIAPGFIETEMTDVLSEDVKENLLGSIPLKRMGQTKDI
                     AETVAFLASDKAAYITGQTISVDGGMGM"
     gene            complement(5731..6651)
                     /gene="fabD"
                     /locus_tag="PLC71_02830"
     CDS             complement(5731..6651)
                     /gene="fabD"
                     /locus_tag="PLC71_02830"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRM01613.1"
                     /translation="MGKIVFMFPGQGAQYVGMGKDFYDSFACSKEIFDKANEVLDIDV
                     KKLCFEENEDINITEYTQAAMVTASVAILKKIEEMGLKPDLTAGLSLGEYCALVASDV
                     MSFEDAVKVVRQRGILMQDTVPAGEGAMSAVLGMKKEAIEAVLPDVEGIVTIANYNCP
                     GQIVISGESKAVAEAGEALKEAGAKRVLPLKVSGPFHSPMLKPAGEKLLDVLADVEVD
                     DPTVPYVSNTTAEFITSNDQVKELLGRQVYSSVCWEQSIEKMIADGADTFVEIGPGRT
                     LCGFMRKIDRNVKAINIAKVEDLEKLKEIM"
     gene            complement(6765..6998)
                     /gene="acpP"
                     /locus_tag="PLC71_02835"
     CDS             complement(6765..6998)
                     /gene="acpP"
                     /locus_tag="PLC71_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRM01614.1"
                     /translation="MLEKMKEIIADQLGVSEDEVTLEASFKEDLDADSLDLFELVMAL
                     EEEYDVEIPSDDLAELNTVGDVINYLKDKGVED"
     gene            7250..8272
                     /locus_tag="PLC71_02840"
     CDS             7250..8272
                     /locus_tag="PLC71_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM01615.1"
                     /translation="MICFSSYNDESYRKKEDNILIEVKHLTKKYGTHLAVDDLSFTIE
                     KGRIYGFLGPNGAGKSTTMNIMTGYIGASSGEVIINGHNTLEVPEKAKKCIGYLPEIP
                     PLYLDMTVSEYLYFAAELKKIPKADRNAQVIKVMELIKLVDMKDRLIKNLSKGYRQRT
                     GLAAAILDFPDIIILDEPTVGLDPKQIIEIRELIRTLAKDHTVILSSHILAEVQEICD
                     DILIISKGKLIAEGSPDELEELTHGKETIELTILADETSVKQVLSDFSDIDDVNWLDV
                     SKDSCHIQINTNKDSQEICADLSLKFAQKKMPVTQMFVNKATLEDIFLELTEDVMEVT
                     NNACYL"
     gene            8256..9122
                     /locus_tag="PLC71_02845"
     CDS             8256..9122
                     /locus_tag="PLC71_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM01616.1"
                     /translation="MLAIYKREVRSYFNSMIGCVFAAFLTIIGGVYFMVYNLYSGYPF
                     FAYSLAGVIFMLLISVPVLSMKSFAEDRKNKTDQLLLTSPVSLVEIVLGKYLAMVTVF
                     AIPCVIYCIFPLIIKLQGTAHFLVDYSSILAFFLLGCVFIAIGMFLSSLTESPVIAAI
                     TTCAALFFFYLLDNLLQYFPSSAISNLVIWIIIFALIGLLIYHITKNQMIAGIVTGIG
                     CIAGIVVYFVKKTLLESLFSTLLGHLVLTDIFYNFSQNYIFDLGGLLTYLSLIILLVF
                     LTVQTFEKRRWS"
     gene            9135..10547
                     /locus_tag="PLC71_02850"
     CDS             9135..10547
                     /locus_tag="PLC71_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HRM01617.1"
                     /translation="MDKIKKLFQSNDSKRGSYTLAMTSIVIAIVIIFNMIVNLIPENK
                     RQFDISSTNIYEISSKSEKIIKKVDDDVTFYVLAEKDSTDKRIKTFINKYASLSSKLH
                     VKWIDPVLHPSALTKYGTEENNIVISCKKTNRTTTVSFDDILVSSDSYYSTSSSASSF
                     DGDGQLTSALNYVTSDKEYKAYYTSGHGESSLSSEVTSLLTKSRISTSDLLLMTATSI
                     PDDCDLLIIDGATSDFTKDEVKLLSSYLKKGGKVVTLLAQTNKSMKNLYGLLKDYGLT
                     VQSGYIADTERSYQGNYYYLIPNLSVSGDMASGISSNSVMMINSKGMTQSTPVRDSIS
                     TDAFMTTSSNGYAVTEKKQTQGTYVLGATSTESVKVKNSKGKKVTKESRLTVYGSNML
                     IDEQITSSFSSLENLTLFTNSVTACLNNADNVSISPKSLEVSYNTIAHPGPFSILVVF
                     VIPVGLIIGGFIVWFRRRRR"
     gene            10557..>11375
                     /locus_tag="PLC71_02855"
     CDS             10557..>11375
                     /locus_tag="PLC71_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRM01618.1"
                     /translation="MKQKKTLQILLLVFAGLIVLYTGVNLYQKQQSSKEDSDQTMISD
                     LKSLTSISYNNNGTTLSFIKQNGKWYYTKNKKYPITQSSLESLASTFQKVEAVRELKN
                     ADALSDYGLDKPTYTVSVKDKTGKSMTYYIGNATGENYYVTYGDKSKIYTVSSDITSS
                     LSYSLDNLIETDTFPTLSTGNLKKVLVTQNGKTKTYTSKKKNLDGISGGLGVFTFGDC
                     QNYYTSQTDLVKYGLDKDSRITVDITYKDTDTKKTKNVVLYIGKKDSDQKNYYVQ"
BASE COUNT         3158 a         2534 c         1863 g         3810 t
ORIGIN      
        1 actccacact tcatttccaa cagccatagc caaggctcca ccacttcctc cttctccgat
       61 catgatagaa agaatcggta ctttgatgtt tgacatctcg aataagtttc ttgcgattgc
      121 ctctccgact ccgccttctt ctgcctctaa tccacagaat gctcctggtg tgtctacaaa
      181 gagaatgatc ggacgatgaa atttctctgc ctgtttcatc agtcttaggg attttctgta
      241 tccttctggt gatggcattc caaaatttcg tttgatattt tctttggttg agcgtccttt
      301 ttgctgtccg atgactgtca ctggtcttcc tccaatagag gctaatccac caacgatcgc
      361 tttgtcatct ccaaaatgac ggtctccgtg taaggacata tatcgttcaa aaatcgtaga
      421 aatataatca agacttgttg ggcgttctgc ggatcttgag atctgcactc tctcccatgg
      481 tgtataatta ctgctcatat tcacaactcc ttatgatcgt taccagcact tttcttaatt
      541 ttttgcgctc tacgatctta tctacaaatc catgttctaa caaaaattct gctctctgaa
      601 atccttctgg taacttctgc ccgatcgtct gttcaataac tctcggtccc gcaaatccga
      661 tcaatgctcc tggttctgct aagataacat ctccaagcat tgcaaaactt gctgtaacac
      721 ctccagttgt aggatctgtt aatactgtga tatataataa tcctgcttca tcatggcgtt
      781 tcaatgctgc ggatgttttt gccatctgca tcagagatac gattccttcc tgcattctag
      841 ctccaccaga gcatgtgaaa atgatgatcg gtaatctcat ctgtgttgca cgttcaactg
      901 ctcgtgtgat cttctcacca acaacttctc ccatgcttcc catcatgaaa tctgctccca
      961 tgactgcgat cacaacatct tttccaagaa tctttccttt tcctgtgatg accgcttcat
     1021 caagattcgt tacggagcgc tggcgttcca atttattctt atatcctggg aagttcagtg
     1081 gatcgcttgt atccatcttt gcatcccatt cttcaaaagt atctttatct aataccatgg
     1141 caattcttgt ctttggacgg attctaaagt aattgccaca gactggacat acataatcat
     1201 tttcgatcaa atcctcattg tatacaggct ttccgcaaca gttacacttc tggaataatc
     1261 cttctggtgc ctgaaattca aggagcgtgt cctttgcttt tcgtacacgc tctgcctgtt
     1321 tgatctcctg aggtgataat gtccttttct taaacatatc ttttaatgac atgatactca
     1381 ccttaacttt ctttattcag cttcattttc agactgatgt ttcttctgaa aacgttcaat
     1441 aaatcctgta tcaacattgc cttcgataaa atcttcatca ttgatgatct catactgata
     1501 gtcaatgttt gtcttcacgc cttctaatac aagttctccc aatgcattac ggagtttgcg
     1561 aagtgccaac ttacgatttt tatcatatac gatcagtttt gcgatcatag aatcatagta
     1621 tggtggaatc tgatatcctg cgaataatgc agaatcaatt ctcactcctt ttcctcctgg
     1681 aaaatgtaca tttgttacag ttcctggact tggacggaag tcatgttctg tgtcttccgc
     1741 attgattctt acttcgattg catgtccatg aacatgtaca tcttcctgtg tataagtcag
     1801 atgttttcct gcggcgatac ggatctgttc acggatcaga tcgattcctg taacccattc
     1861 agtcactgga tgctctacct gaattcttgt gttcatctca ataaaataga aatctccatg
     1921 tttgtcacgt aagaattcaa tcgttcctgc actgtagtat tctgctgctt ttgctgcttt
     1981 gactgcgatc tcacccatct tctttcgctg ttcgtcactg atcgcagggg atggtgattc
     2041 ttcgatcagt ttctgatgtc ttctctgaat agaacaatct ctttctccaa gatgaatcgt
     2101 atttccgtat ttatcagcaa gaatctgnnn nnnnnnncag atggatcgcg ttgccgcgcc
     2161 cgtcgccgaa aatctggaat tcgatatggc gtggatcttc gatatatttt tcaatataca
     2221 tcgttccgtc gccaaatgca ttctctgcct ctaacttcgc tgtctcaaaa gtatgtacga
     2281 agtcttcaga gtcatagcag acacgcattc cctttccacc acctcctgcg gaagctttga
     2341 tcataactgg gtatcccatg tcatctgcga tcttctgccc tgcttctgcc tcataaacgg
     2401 catcttttgt accaggaact actggaactc cagcatccat cattgtcttt cttgctgcgg
     2461 atttgttacc catgcggtca atgatttctg cggatggtcc aataaatgtg atattgcatt
     2521 tttcacacat tcttacaaat ttgctattct cagacaaaaa tccgaatccc ggatgaattg
     2581 catccgctcc gactgccatt gcagccccaa gtacacgttc catgtcaaga taactgtctt
     2641 tggcaggtgc tggtccgata cagatcgtct ggtctgccag ctgggcatgg agtgcatctt
     2701 tatctgcttc agaacagatt gctacggaca tgatatccat ctcacggcac gcacggatga
     2761 tacgcactgc gatctctcct ctgtttgcaa ttaaaatctt cttaaacatt taagaaaccc
     2821 tctcttatcc aatcataaat gtaatctctg cagatgccac tttcttacca tctacagttg
     2881 ctgttccctg tgcaacaccg actggtcctt tgactttgat catctcaact tctaatgtca
     2941 gcacatctcc tggaacaacc tttcctctga attttgcttt gttgactcca cctaagaatg
     3001 caatctttcc tttgtactca tctgcagaaa gtgcacatac agctccagtc tgtgctaatg
     3061 cttcaatgat cagtacccca ggcatcactg gttcctgtgg aaaatgtcct gcaaaaaatg
     3121 gttcattata tgtcacacat tttttaccaa cgatacgttt tccttcttcc atctcctcaa
     3181 tacggtccac taataaaaat ggacttctgt gagggatcgt attcataata tctttaatat
     3241 ccattaccat aataaatatt ctgcctttct tactggatga ccattaatgg ctgtccatat
     3301 tcaaccatct caccattctc aactaaaata gctgcaactg ttccatcgta ttcactttcc
     3361 acttcattca tcagtttcat tgcttcaacg attccgatta cctgtccttt tttgactttg
     3421 tcaccgacag atacaaatgg ctcagctcct tctgatggtg ctacataaaa tgtaccaaca
     3481 agtggggctt tgatctcatc tccatagaga agctcttctg gctgttcttc attctgttct
     3541 ggcatgatcg cggcactctg tactggtact cctgcaacag atgcatcaac tgttgcgatc
     3601 tttgcctttt tcttagcgat tctgattgaa aattcttcgt tattatattc aaattcatcg
     3661 acatttgcag aagatactgc agcgatcaat tctttcattt ctttaacttc catgtcagtc
     3721 tccttctaaa ttaatcctca aatttcttca caagaagtgt tgcgttatgt cctccaaatc
     3781 caagagaatt gctcataacg taatttacct tgcgatctgt tactggttct aatgtataat
     3841 caagatcaca ttcttcatct ggaaccttta atcctacggt tgggtgaata taatcttctt
     3901 caattgattt aacacatgtg ataaattcaa tcgcaccagc tgctccaagt ccatgaccga
     3961 tcatagattt tgtagagctg atcttcacat catatgcatg ttctcctaat gctttcttga
     4021 ttgcttttgt ctcaaataag tcatttgcat gagtacttgt tccatgtgca ttgatataat
     4081 caactttatc tggtgtgatt cctgcttcat ccattgcaaa taccatagct cttgctgcac
     4141 cttctccatc ttcacatggg gatgtgatat ggaatgcatc acttgttgca ccatatccga
     4201 caacttctgc taaaatatct gctcctcttg cctgtgcatg ttcaaggctt tctaaaacaa
     4261 cgattcctga tccttctcca atgacaaatc catctctctc tttgtcaaat ggaatagatg
     4321 ctctcttcgg atcatcagat ccacttaaag ctgttaacgc tgcaaatcca ccaactgcaa
     4381 cttctgatac tgcagcttct gttccaccgg ctaccattac atcagcttct ccaacctgaa
     4441 tggatctata tgcttcacca atagactgtg ttcctgtcgc acatgctgta acaacgttga
     4501 tactctttcc gcgaaggcca aatgcaatag aaacatttcc tgcagccatg ttagagatca
     4561 tcattggaac tgctaatggt ttcactctgt tcggaccctt tttatctaat gttcttgttg
     4621 ttgtttcgat catctgaagg cttccaactc cacatccaac agatacacca attcttgttg
     4681 tatcttcttt ctcaagatca agtcccgcat cttcgattgc ttcttttgtt gcagctacag
     4741 catactgtga aaataattcc atacgtcttg ctgcttttgg tgacatataa tctttggcgt
     4801 tgaaaccttt gacttctgca gccagatgag ctttgtattc tgttgcatca aaatatgtaa
     4861 tcggtgcaaa tccgatcttc tgctctttga ttccattcca aaattcttcc acgctgtttc
     4921 cgatcggtgt gatcgcaccc ataccagtta caacgacacg tttcatgctc ttcatataaa
     4981 atctccttta cattcccatt ccaccgtcaa cactgatcgt ctgacctgta atatatgctg
     5041 ccttgtcaga tgctaagaaa gcaactgtct ctgcaatatc ttttgtctgt cccattctct
     5101 ttaatggaat gcttcctaat aaattctctt tcacatcttc tgacagtaca tccgtcatct
     5161 ctgtctcaat aaatcctgga gcgatcgcat tgaccgtgat tcctctggaa cctaattctc
     5221 ttgccagaga ttttgtcata ccaatgattc ctgcttttga tgcacagtaa ttgacctgtc
     5281 ctgcattacc cataactcca gatacagaag acatattaat gatatgtcca gatctctgtt
     5341 ttaacatgat tcttgaaaca tgtttcatgc agttaaatgc accttttaag tttacttcaa
     5401 tgactttatc aaattcttct tcactcatct tcatgatcag gttatctttt gtgattccgg
     5461 cgttattcac cagaatatca atagacccaa attctttctt tacagaagtg atcatctctt
     5521 tcgccacatc aaaatcggat acatctccct gatatgcttt tgctgttccc ccagctgctg
     5581 tgatctcttc tacaactgcc tccgctttat ctttggaacc acagtaattt acgatcacag
     5641 ttgctccata tcctgcaaga gtcagtgccg tttctcttcc gatcccgcgg cttgcacctg
     5701 ttacgattgc aacttttcca cttaacatga ttacatgatc tcctttaatt tctctaaatc
     5761 ttctactttc gcaatattga tcgctttgac attacgatcg atcttgcgca taaatccaca
     5821 taatgttctt cctggtccga tctctacaaa tgtatctgca ccatcagcga tcatcttctc
     5881 aatagattgt tcccagcata cagaagaata aacctgtctt ccaagcaatt ccttaacttg
     5941 atcattactt gtgataaatt ctgctgttgt attggatacg tatggaactg ttggatcatc
     6001 cacttcaaca tctgctaata catcaagcaa tttctctcca gctggtttta acattggaga
     6061 atgaaatggt ccactgacct tcagtggcag aactctctta gctcctgctt ctttcaacgc
     6121 ttctccagct tctgcaaccg cttttgattc tcctgaaata acgatctgtc caggacagtt
     6181 atagtttgcg atcgtaacga ttccttcaac gtctggtaaa actgcttcga tcgcttcttt
     6241 cttcattcca agaaccgctg acatcgcacc ctctcctgct ggaacagtat cctgcataag
     6301 aattcctctc tgccttacga ctttcactgc atcttcaaag ctcatcacat cagatgctac
     6361 aagtgcacag tattctccaa gacttaatcc cgctgtcaga tcaggcttta atcccatttc
     6421 ttcgatcttc tttaagattg caacacttgc agttaccatc gctgcctgtg tatactctgt
     6481 aatattgata tcttcattct cttcaaaaca aagtttcttt acatcaatat ccagaacttc
     6541 attcgccttg tcgaaaatct ccttgctgca tgcaaaagaa tcataaaaat cctttcccat
     6601 tccaacatat tgcgctccct gtcccggaaa cataaatacg atcttaccca ttatctttac
     6661 ctcgttgatc tataaaataa tctataatta gtttgatgtt caaaatattt tcttcaaaaa
     6721 attgacttat aacaggaatg ttataagcca atcttacaat ttctttagtc ttctacacct
     6781 ttgtctttta agtagttgat aacatcacca actgtgttta attctgcaag atcatcagat
     6841 ggaatctcaa catcatattc ttcttctaat gccataacta attcaaataa atctaatgaa
     6901 tcagcatcca gatcttcttt aaaagatgct tctaaagtaa cttcatcttc gcttacacct
     6961 aactgatctg caatgatctc tttcatcttc tctaacataa tgattctcct tcacactcta
     7021 attagtttga ttctcaaagt atacattctt caactgtctt ctgtcaagtt ttcttgtaaa
     7081 tttactttta tattatacga aactatacgt gattatgcaa gcctatcctt gccattctat
     7141 gcatctttta tgaaacattt tcatattatt ctgtatacta tatactccat aaaacacttc
     7201 acaaaattac cacattttta acacaaactg ttcagacttt tatcacagtt tgatttgttt
     7261 ttcttcatat aatgatgaga gttatcgcaa aaaggaggac aatattttga tagaagtaaa
     7321 acatcttacc aaaaaatatg gcacacatct cgccgtagat gatctgtcat ttaccattga
     7381 aaaagggcga atctatggat ttttgggtcc aaatggagcc ggtaaatcta caactatgaa
     7441 tattatgact ggatatatcg gtgcgtcgag tggagaggtt atcatcaatg gtcacaatac
     7501 tctggaagta cctgagaaag caaaaaaatg tatcggatat cttccagaga tcccaccttt
     7561 atatcttgat atgacagtat ctgaatatct gtattttgct gctgaattaa aaaaaattcc
     7621 gaaagctgac cgcaatgcac aggttataaa agttatggaa ctgatcaaac ttgttgatat
     7681 gaaagaccga ttgatcaaaa acctttccaa aggttatcgc cagagaacag gtcttgccgc
     7741 tgcaattctt gatttcccgg atattatcat cttggatgaa ccgaccgttg gtctcgatcc
     7801 aaagcagatt atcgaaatcc gtgaactgat ccgtacactt gctaaggacc atacggtgat
     7861 cttaagttct catattcttg ctgaagttca ggaaatctgt gacgatatcc tgatcatttc
     7921 caaaggaaaa ttgatcgctg agggaagccc tgatgaatta gaagaattaa cacacggaaa
     7981 agaaaccatt gagcttacga tcctagcgga tgaaacttcc gtaaaacagg ttctttctga
     8041 tttttctgat attgatgatg tcaactggct tgatgtaagt aaagatagct gccatatcca
     8101 gatcaataca aacaaagatt cacaggaaat ctgtgctgat ctttctctta aatttgcaca
     8161 gaaaaaaatg cctgtgacac agatgtttgt aaacaaggct actcttgaag atattttcct
     8221 agaattaacc gaagatgtaa tggaggtgac aaataatgct tgctatctat aaaagggaag
     8281 tccgttccta ttttaactct atgatcggat gcgtatttgc agcattctta acgatcattg
     8341 gaggtgtata ttttatggtt tataaccttt atagtggtta tccatttttt gcctactctc
     8401 tggcgggcgt tattttcatg ttattgattt ctgtgcctgt tttatctatg aaatcttttg
     8461 cagaagaccg aaagaataaa actgaccagt tattattaac ttctcctgtt tcacttgttg
     8521 agatcgtatt aggaaaatat cttgcaatgg tgactgtatt tgcaattcca tgtgtgatct
     8581 actgtatttt cccactgatc atcaaacttc aaggaactgc ccattttctt gttgattatt
     8641 catccattct ggcctttttc ctgcttggat gtgtatttat tgcaatcgga atgtttttat
     8701 catctttgac agaaagtcct gtgatcgcag cgatcactac ttgtgcagca ttgtttttct
     8761 tttatctgtt ggacaatctg ctgcagtatt ttccttccag tgcgatcagt aatctggtca
     8821 tctggatcat tatatttgct ctgatcggtc ttctgatcta ccatatcaca aaaaatcaga
     8881 tgatcgctgg cattgtcact ggcattggat gtatcgctgg tatcgttgtt tattttgtga
     8941 agaaaacatt gcttgaaagt ctgttttcta ctttattagg acaccttgta ttaactgata
     9001 ttttttataa tttttctcag aattacattt ttgatctcgg aggacttctg acttatctgt
     9061 cattgatcat tcttctggtg ttcttaacag tacagacatt tgaaaaaaga cgttggagtt
     9121 aggaggaagt tatcgtggat aaaatcaaaa aattatttca aagcaatgat tcaaaacgtg
     9181 gatcttatac tcttgctatg acatccatcg tgatcgcgat cgttatcatt tttaatatga
     9241 tcgtcaatct gatccctgaa aataagcgac agtttgatat cagtagtacc aatatttatg
     9301 aaatctcatc aaaaagtgaa aagatcatca aaaaagttga tgatgacgtc actttttatg
     9361 ttcttgcaga aaaggacagc actgataaaa ggatcaaaac tttcatcaat aaatatgctt
     9421 ctttatcaag caagcttcat gtcaaatgga tcgatcctgt gcttcatcca tctgcattga
     9481 caaaatacgg aaccgaagaa aacaatatcg tcatctcttg taagaaaacc aaccgtacaa
     9541 caactgtttc gtttgatgat atattagttt cctcagattc ttactacagt acttcctctt
     9601 ccgcttcatc atttgatggg gatgggcagc ttacaagtgc tttaaattat gtaacaagtg
     9661 acaaagaata taaagcttat tatacttctg gccatggaga atcatcctta tcttccgagg
     9721 tcacaagtct tcttacaaaa tcaagaattt ccacaagtga tctgctttta atgactgcaa
     9781 catccattcc tgatgattgt gatctgctga tcatcgatgg ggctacaagc gattttacaa
     9841 aagatgaagt aaaactatta tcttcttacc tgaagaaagg tggaaaagtt gttactttac
     9901 ttgcccagac aaataagagt atgaaaaact tatatggtct gttaaaagac tacggtttaa
     9961 ctgttcaaag cggatatatt gcagatacag aacgttcata tcaaggaaat tattattact
    10021 tgatcccaaa tctttctgta agcggtgata tggcttctgg aatttcatca aactctgtta
    10081 tgatgatcaa ttcgaaaggt atgacacaga gtactccagt tagagattct atttctaccg
    10141 atgcatttat gacaacatcc agcaacggat atgctgtcac tgagaagaaa caaacacagg
    10201 gaacttatgt ccttggtgca acttccacag aatctgtcaa agtcaaaaac agcaaaggaa
    10261 agaaagttac aaaagaaagt cgcctgactg tttacggaag taatatgctg atcgatgagc
    10321 agatcacaag ttctttctca tccttagaaa acctgacatt atttacaaac tctgttaccg
    10381 catgtttaaa taatgccgat aatgtttcta tctctccaaa gagtctggaa gtcagctata
    10441 atacgattgc tcatcctgga ccattcagta tccttgtagt ctttgtaatt ccagttggtc
    10501 tgatcattgg cggattcatt gtatggttta gacgcagacg ccgttaggag gttttcatga
    10561 aacagaaaaa aacattacag attttacttc tcgtatttgc aggacttatt gtcctgtata
    10621 cgggagtgaa tttataccag aaacagcaat cttccaaaga agattctgat cagacaatga
    10681 tctctgattt aaagagttta acttccatct cttataataa caacggaacg actctttctt
    10741 tcataaaaca gaatggcaaa tggtattata caaaaaataa aaaatatccg atcactcagt
    10801 cctctctcga aagtcttgct tctacatttc aaaaagttga ggctgtcaga gaacttaaaa
    10861 atgcagatgc tctaagcgac tatggtcttg acaagccaac ttatacagtt agtgtcaaag
    10921 acaaaactgg aaaatccatg acttattata ttggaaatgc aaccggtgaa aattactatg
    10981 taacttatgg agataaatca aagatttata ccgtatcttc tgatattaca tctagtttat
    11041 cttattcttt agataatctg atcgagacag atactttccc tacgttaagt actggaaatc
    11101 ttaaaaaagt tttggtcact cagaacggta agacaaagac ttatactagc aagaagaaaa
    11161 atcttgatgg aatctctggc ggtttgggtg tatttacttt tggagactgc cagaattatt
    11221 atacgagtca gacagatctt gtaaaatatg gactggataa ggattctcga atcacagtcg
    11281 atatcactta taaggatacg gatacgaaga aaacaaaaaa tgttgtgctt tatatcggta
    11341 aaaaagattc tgatcaaaag aattattatg tacag
//
LOCUS       DAOMUE010000033        10708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1493_length_10708_cov_2.654088, whole genome shotgun sequence.
ACCESSION   DAOMUE010000033 DAOMUE010000000
VERSION     DAOMUE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10708
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1493_length_10708_cov_2.654088"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLC71_02860"
     CDS             complement(<1..750)
                     /locus_tag="PLC71_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HRM01619.1"
                     /translation="MSILFLILKIIGILILIPIVLLLILLIFPICYQIEGDFDGKSPK
                     AQVKVSWAVIFLRAKAFYEEELNFGIRIFGIPIYDSRKEHWSVFGEHKKKDKKKHKKR
                     SDSKKKQKNKKNNKKRVEKKKSTYPSEDEDVFNLTWDEKEERTKTSTTRKMYERSEDK
                     ISESKIDKTTDKEKEFFGKRIVNFLKKCYNRCKNFITKIRKITNKMEMIGDLLEDEDI
                     IDAVKRIKRYGVNGVKLLLPQKLNAKIIFGFE"
     gene            complement(747..1064)
                     /locus_tag="PLC71_02865"
     CDS             complement(747..1064)
                     /locus_tag="PLC71_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01620.1"
                     /translation="MKVSERVYISEILKLQKKRLFCKIQEKKSISRLYCITLPLWNSA
                     FLEIYPYNELLSDIHEGKDITIVGMAAGRDDAVVMLRRMIDDLYHKGELEDVTTYFCG
                     EDK"
     gene            complement(1171..2613)
                     /gene="pyk"
                     /locus_tag="PLC71_02870"
     CDS             complement(1171..2613)
                     /gene="pyk"
                     /locus_tag="PLC71_02870"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRM01621.1"
                     /translation="MLTKKTKIICTMGPATDDDEVLKDLMRSGMDIARLNFSHGDHEE
                     QLGRIKRIKKFREELNLPIAILLDTKGPEIRTGLLETDDDVELVTGQEYTLTTRDIKG
                     NNEITSITYAELPQDVEAGNTILIDDGLIELKVKEIKDGTDIVCDVINGGLLGSRKGV
                     NVPNVRVNLPSITEKDKADIEFGLENGIDFIAASFIRNAEAVEEIKDIIGAHNMHVGV
                     ISKIENMEGVENIDAIIDASAGIMVARGDLGVEVPAEEVPFLQKEIIRKCNDAFKPVV
                     TATQMLDSMIRNPRPTRAEATDVANAIYDGTDAIMLSGETAKGKYPVEAVKMMNQIAI
                     STEVHLSTKIKDYRSKYINRGISAAVAYSAVQTAHNVNAKCILASSMSGFTTRIVSKF
                     KPDAQIIGLSPDDAIVRQMQIYWGVRPFKSHKAETTKDLLDEATDIVLDAGLAEKDDI
                     LVLTGGGPANHRGKGVTNMLKVVKIGEFGE"
     gene            complement(2660..3529)
                     /locus_tag="PLC71_02875"
     CDS             complement(2660..3529)
                     /locus_tag="PLC71_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01622.1"
                     /translation="MWLNKLEEKFGRYAVPNLPKIIVLLYAVGFVIANISPRLYSLLE
                     LNPYLILHGQVWRIVTFLLIGPETNLIFVIFVLLFYYSIGSSLEQVWGTFRFNMYYLI
                     GVLGTIVGAFLTYAILTFAYGEGYGAFVNMDTFYLNMTLFLAYASMFPEMEVYLYMIL
                     PIKVKWLGYLDGLYLIYIFLSSGFTVAGISVKVSIVAALLNFLLFFFSMKKIRRMGAN
                     FKAKTIHKKKARAYKAKTITPKKKNGAMHECAVCHRTELDDPELEFRYCSKCDGNYEY
                     CQDHLFTHKHVKR"
     gene            complement(3555..4487)
                     /locus_tag="PLC71_02880"
     CDS             complement(3555..4487)
                     /locus_tag="PLC71_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRM01623.1"
                     /translation="MIKYYRTMDHQIHEISEPMEGTWISLIHPTAAELAKIATDYKID
                     IDDLRAPLDEEERSHIEVEEGYTLFIVDVPTTEERKEKEYFLTIPCGIILTEKVIITV
                     CLEDTAVLADFKNGRVRNFWTFKRTRFILQILYRNASLYLQYLRIIDKKSDSVEKQLH
                     ISTKNQELIELLELEKSLVYFSTSLRSNELVLEKMLKIDKIKQYPEDEELLDDVIIEN
                     KQAIEMADIYSNILSGTMDAYASVISNNLNIVMKVLAIITIVMSIPTMIASFWGMNVP
                     VPLAHSAHGFLILVIVSLILTIFGGYILGKKNMF"
     gene            complement(4612..4956)
                     /locus_tag="PLC71_02885"
     CDS             complement(4612..4956)
                     /locus_tag="PLC71_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRM01624.1"
                     /translation="MMQTNSCKGTVYVVKEKDSLYKIAKAHGVRVKDIMRQNPFVNVY
                     NLQIGDELCVPGFTNVIEGAFIPYVVKKGETILDIAKMHKTTVENLAKSNKKIRELTL
                     PVGTVLLIDKAK"
     gene            5173..9390
                     /locus_tag="PLC71_02890"
     CDS             5173..9390
                     /locus_tag="PLC71_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HRM01625.1"
                     /translation="MKRLGIDIGSTTVKVAVIDEQHNILFSDYQRHFAKIQETLSSLL
                     KKAKDQIGEMTFAPTVTGSGGLSISSYLDIPFCQEVVCVSSALQDYAPQTDVAIELGG
                     EDAKIIYFTNGIDQRMNGVCAGGTGSFIDQMASLLQTDAGGLNEYAKDYDTIYPIAAR
                     CGVFAKTDIQPLINEGATKPNLAASIFQAVVNQTISGLACGKPIRGNVAFLGGPLHFL
                     TELKEAFIRTLNLKDDEIIAPTHSHLFAAVGAALNAKEEVTTDFEHLLKQFEKKIELQ
                     QEVDRLEPLFKSEQEYKNFVKDHNRHVVKRGDLATYKGNCYLGIDAGSTTTKVALAGE
                     DGELLYSYYNNNNGSPLHAVVEALHEIDAQMPKTAKIVSSCSTGYGEHLVKAALNLDF
                     GEVETIAHYYAAAFFDPDVDCILDIGGQDMKCIRIKNGVVDDVQLNEACSSGCGSFIE
                     TFAKSLNHSVQEFAKVALTAQNPIDLGSRCTVFMNSKVKQAQKEGATVGDISAGLAYS
                     VIKNALYKVIKLTDPSDLGKHIVVQGGTFYNDAVLRSFERISGCHAVRPDIAGIMGAF
                     GSALIARERYEDGMETSMLPLEEIFAMSVDTSMTRCKGCTNHCLLTINKFSNGRRYIT
                     GNRCERGIGKEPNAENIPNLYDYKLHRTFDYEPLSDEKATRGVIGIPRVLNIYENYPF
                     WYTFFTDLGFKVVVSPESSRKIYELGIESIPSESECYPAKLAHGHVMWLIKQGIKDIF
                     YPCIPYERDEMEGTNNHYNCPIVTSYAENIKNNMEELATEHINFMNPFLALDNEEALK
                     SRLFEELEAQYHLTADEINHAVDKAYAELSQVRTDIQNKGEEVLAYLAETGRTGIVLC
                     GRPYHIDPEINHGIPELINSYGIAVLTEDSISHLSKVERPLNVQDQWMYHSRLYAAAN
                     YAKANKQLEVIQLNSFGCGLDAVTTDTVKDILTKSGRIYTVLKIDEVNNLGAARIRIR
                     SLISAVRVRKKHKIEPKPVSPAIKRVEFTKEMRKNYTLLVPQMSPIHFEFLEPALQSC
                     GYNVEVLNQGGMEDVNTGLKYVNNDACYPSLIVIGQLMNALLSGKYDPHKIALVISQT
                     GGGCRATNYISFIRRALEKAGYGYVPVVGLSAQGIEKNSGFSFTPGLLNKAVQAIVYG
                     DVFMRTVYHTRPYEVKPGSVNKLHRQWAAKCKRDIAKGNFYTFQKNIRGIIRDFDRIP
                     LKDIKKPRVGIVGEILVKFLPDANNHLVELLEREGAEAVVPDLLDFFMYSFYNSNFKY
                     RYLGKSKKSAIVSNSAIWIVERYRQTLRKELAKSNRFEPPAYIKDLAEYAKPFVSIGN
                     QTGEGWFLTGEMVELIHSGAPNIVCTQPFACLPNHVVGKGVIKEIRQAFPDANIVAID
                     YDPGASEVNQINRIKLMLSAAEKNMNK"
     gene            complement(9509..9916)
                     /locus_tag="PLC71_02895"
     CDS             complement(9509..9916)
                     /locus_tag="PLC71_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HRM01626.1"
                     /translation="MSIKTDKAAQNHQKGYNCAQAVACAFAEEMGIDESIIFKMMEGH
                     GLGMGCMEGTCGAITGAVAVIGAIKSCGDIEKPVSKAATYKLSGELVKRFQEKNQATK
                     CKDLKGIETGKVLRSCPGCIEDATAILEEILAE"
     gene            complement(9936..>10708)
                     /locus_tag="PLC71_02900"
     CDS             complement(9936..>10708)
                     /locus_tag="PLC71_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRM01627.1"
                     /translation="EIKDPFTSGENKEEKEGILDIKLTLNGNKKINIEMQNTYQEDWT
                     ERSLFYNCRMFTEGFKKGEPYWKLSPCIHVGILNFNMMKSPGYYHKVTLRDEKTNELY
                     SRKFQFHMIELKKTKTAKGKARKHPLYRWARLIAATTWEEVAQESAGNRYMERIQEEM
                     VKMSQDERDRYLYLREEMAASDRVSQLQSAENRGVRAGKLLNQISMIQKKVKKNKNLE
                     QIADELEESTTKIRPIYDQVKQHPDKTAEEIYNLINNE"
BASE COUNT         2965 a         2210 c         1879 g         3654 t
ORIGIN      
        1 ttcgaatcca aatataatct ttgcatttag tttctgtgga agtaacagct ttaccccatt
       61 cactccatat ctcttgatcc gcttcactgc atcaatgata tcctcatctt ccaatagatc
      121 tccgatcatt tccatcttat ttgtgatctt acggatcttt gttatgaaat tcttgcatct
      181 attataacat tttttgagaa aattcacgat tcttttgcca aaaaactctt tctctttatc
      241 tgttgttttg tctatcttag attctgatat cttatcttct gatctttcgt acatttttct
      301 tgttgttgat gtctttgttc tttcttcttt ttcatcccat gtgagattaa atacatcttc
      361 atcttcagaa ggatatgtac tttttttctt ttctactcgt ttcttattgt tttttttatt
      421 tttttgtttc tttttagagt ctgatctttt cttatgtttt ttcttatctt tctttttatg
      481 ctctccaaat acagaccagt gttctttcct tgaatcataa atcggtattc caaaaatcct
      541 gatcccaaaa ttcagctctt cttcatagaa tgcttttgca cgtaaaaata taactgccca
      601 tgataccttt acctgtgctt tgggactctt cccatcaaaa tctccttcga tctgataaca
      661 gatcgggaag attaaaagga tcaacaataa cacgatcggt atcaggatca ggattcctat
      721 gatctttaat attaaaaata gtatactcat ttatcttccc cacaaaaata tgttgttaca
      781 tcctcaagtt ctcctttatg atacagatca tcgatcatgc gtcttaacat aacaactgca
      841 tcatctcgtc ctgctgccat tcctacgatc gtgatatctt ttccttcgtg gatatcagat
      901 aatagttcat tatatggata tatttccaga aatgcactgt tccagagcgg cagtgtaata
      961 cagtacaatc ttgaaattga tttcttttct tgtattttac aaaatagacg tttcttttgt
     1021 aattttaata tctctgatat gtatacacgt tctgaaactt tcactcattt ttcctcctga
     1081 aaattctccc cattcatttc tgataaaatt cctgataaaa caaaaaggga tgtattacta
     1141 catccccatt ttaacatata ttgttgtcga ttactcacca aattcgccga tttttacaac
     1201 tttcagcatg tttgtaacac cttttccacg atggttagct ggtccaccac ctgttaagac
     1261 taagatgtca tctttctcag caagtcctgc atctaatacg atatctgttg cttcatctaa
     1321 cagatctttt gttgtctcag ctttgtgaga tttgaaagga cgtactcccc aatagatctg
     1381 catctgacgt acgatcgcat catctggtga cagaccgatg atctgtgcat ctggtttgaa
     1441 tttagatacg attcttgttg tgaatcctga catacttgat gctaagatac attttgcatt
     1501 tacattgtgt gctgtctgta ctgcagaata agcaactgct gctgaaattc cacgattgat
     1561 atatttagat ctgtaatctt tgatctttgt gcttaagtga acttctgttg agattgcgat
     1621 ctgattcatc atcttaacag cttctactgg atatttacct tttgctgttt ctccagataa
     1681 catgattgca tctgttccat catagatcgc atttgccacg tctgtagcct cagctcttgt
     1741 tggacgtggg ttacggatca tagaatctaa catctgtgta gctgtaacaa ctggtttgaa
     1801 tgcatcgtta cattttctga tgatctcttt ctgtaagaaa ggtacttctt cagcaggtac
     1861 ttcaacacca aggtcaccac gggcaaccat gataccagca gatgcatcga tgatcgcatc
     1921 aatgttttca acaccttcca tgttctcgat cttagagatt acaccaacat gcatgttatg
     1981 tgcaccgatg atatctttga tctcttctac agcttctgca ttacggatga atgatgctgc
     2041 gatgaagtca attccattct caagaccgaa ttcgatatct gctttatctt tttctgtgat
     2101 agatggtaag ttaactctta cgttaggtac gttaacaccc ttacggcttc ctaaaagtcc
     2161 tccgttgata acgtcacata cgatatctgt tccatctttg atttctttaa cttttaactc
     2221 aattaatccg tcatcgatca ggattgtgtt tccagcttct acatcctgtg gaagttccgc
     2281 atatgtaatg ctggtaattt cattatttcc tttgatgtct cttgttgtca gtgtatattc
     2341 ctgacctgtt actaattcaa catcatcatc tgtttctaat aatcctgtac ggatctctgg
     2401 tccttttgta tcaagtaaaa tagcgattgg taaattcagt tcttctctga acttcttaat
     2461 tctcttgata cgtcctaact gctcttcatg atctccatga gagaagttta aacgagcaat
     2521 gtccattcct gaacgcatca ggtcttttaa tacctcatcg tcgtctgttg caggtcccat
     2581 tgtacaaatg atttttgtct tcttggttag cataattttt ctcctttggt ttcaaaaata
     2641 tatttaaata ataaacaact caccttttca catgcttatg ggtaaataaa tgatcttgac
     2701 aatattcata attcccatcg cattttgaac aataacgaaa ttccaattct ggatcgtcaa
     2761 gttctgttct gtggcacact gcacattcgt gcatcgcacc attcttcttc tttggcgtga
     2821 tcgtcttagc tttgtacgct ctcgccttct tcttatgtat tgttttagcc ttgaagtttg
     2881 ctcccattct tcgaatcttc ttcatagaga agaaaaatag tagaaaattt aacaaagctg
     2941 caactataga tactttaacc gaaattccgg caactgtaaa gcctgaactt aaaaaaatgt
     3001 agattaaata taatccatct aagtatccaa gccattttac cttgattgga aggatcatat
     3061 acaaatatac ttccatctct gggaacatag acgcatatgc taaaaataac gtcatgttca
     3121 gataaaaagt atccatgtta acaaacgctc catagccttc tccatatgcg aatgtaagga
     3181 tcgcataagt taaaaatgcc ccaacgatcg ttcctaaaac tccgatcaaa taatacatat
     3241 tgaatcggaa tgttccccat acctgctcta atgagcttcc gatcgagtaa taaaataaca
     3301 atacaaatat tacaaagatc aaattcgtct ctggtccgat caacaggaat gtcacaattc
     3361 tccagacttg tccatgcaag atcagatacg gattcaattc cagtaaacta tataatcgtg
     3421 gtgatatatt ggcgatcaca aaaccgactg catacaataa aacaatgatc ttagggagat
     3481 tcggtaccgc atatctgcca aacttctctt ccagtttatt aagccacata aaaaaccttt
     3541 cttatcatca aaaattaaaa catatttttc tttccaagga tatatcctcc aaagatcgta
     3601 aggatcaagg atacgatcac aaggatcaaa aatccatgtg ctgaatgtgc cagtggtact
     3661 ggaacattca ttccccagaa actcgcgatc atcgtcggaa tcgacataac gatcgttatg
     3721 atcgcaagaa ccttcattac aatattcagg ttgtttgaga taacggatgc gtatgcatcc
     3781 atcgtaccac ttaagatatt gctgtagatg tctgccatct caatcgcctg tttattctct
     3841 atgataacat cgtccagtaa ttcctcatct tctggatact gtttgatctt atcaatcttt
     3901 aacatcttct caagcaccag ttcgtttgat ctcagggatg tagagaagta aaccaaactc
     3961 ttctctaatt ccagaagctc gatcagttcc tggttctttg ttgaaatatg aagctgcttc
     4021 tcaacagaat cactcttctt atcaatgatt cgcagatact gcagatacaa ggatgcattc
     4081 cgataaagga tctgcaagat aaatcttgtc cttttaaatg tccagaaatt tcgaaccctt
     4141 ccattcttaa agtctgctaa cactgctgta tcttccagac agaccgtaat gatcactttc
     4201 tctgtcagga tgataccaca tggaatcgtc aggaagtatt ctttttcctt tctctcttct
     4261 gttgtcggaa cgtctacgat aaataaagta taaccttctt ccacttcgat atgagaacgt
     4321 tcttcttcat ccagtggtgc tcttaagtca tctatatcaa tcttatagtc tgttgcgatc
     4381 ttcgcaagtt ctgcagctgt gggatgaatt aacgatatcc aggttccttc cataggttca
     4441 ctgatttcat gaatctggtg atccatcgta cgataatact tgatcattgt ccgctccttt
     4501 ctatggaagt accttgtcca taagttcccc acaaacagga ctctatgaat cctgttttat
     4561 catctgaaat atctaaaatt ccaaactttt acctattcca ttataaataa ctcattttgc
     4621 tttgtcaatt aacagtactg taccaactgg aagtgttaac tccctaattt ttttgttact
     4681 ttttgctaaa ttttccactg ttgttttgtg catcttggct atatcaagga ttgtttctcc
     4741 tttttttaca acatatggaa tgaaagctcc ttcgatcacg ttcgtaaatc caggcacaca
     4801 taattcatct ccaatctgta aattatatac atttacaaaa ggattttgtc tcatgatatc
     4861 ttttaccctg acaccatgtg ctttcgcgat cttatataaa ctatcctttt cctttacaac
     4921 atacactgtc cctttacacg aatttgtctg catcattctt ggcattcctt cgctttttta
     4981 tagaatatgc agcagcattc ccttgtttcc attattttct ccgatttttt tagataatat
     5041 tcctatttgc aattattttg aaaatgatta taatagatat aatgtccatg tatattggac
     5101 agatatgttt ttttaacatt attttaacgc ttgaaattat agtactttta tagaaagcag
     5161 gtatacgaga agatgaaacg attaggaatt gatattggct caacgacagt caaggtcgct
     5221 gtcatcgacg aacaacataa tatacttttc tctgattatc agagacattt tgccaagatt
     5281 caggaaactt tatcttcctt attaaaaaag gcaaaagacc agattgggga aatgacattt
     5341 gctccaaccg ttaccggttc cggaggactt tccatttctt cttacttaga tattccattc
     5401 tgtcaggaag ttgtctgtgt atcaagtgca cttcaggatt atgcacctca gacagatgtt
     5461 gccattgagc ttggtggtga agatgctaag atcatttatt tcacaaatgg gatcgatcaa
     5521 cgtatgaatg gtgtctgtgc cggtggtacc ggatcattta tcgatcagat ggcctcttta
     5581 ttacaaacag atgctggcgg attaaatgaa tatgcgaaag attatgatac gatctatccg
     5641 atcgcagccc gctgcggtgt attcgccaaa acagatatcc agcccctgat caacgaaggt
     5701 gctacaaaac caaaccttgc tgcctctatt ttccaggcag tcgtaaacca gacgatcagt
     5761 ggtcttgcat gtggtaaacc gatccgtgga aatgttgctt tcctcggagg acctcttcat
     5821 ttccttacag aattaaaaga agcatttatc cgtacattaa acctaaaaga tgatgagatc
     5881 atcgctccta cacactctca tttatttgca gctgtcggtg ctgccctgaa tgctaaagag
     5941 gaagttacaa ctgattttga acatctttta aaacagtttg agaagaagat cgaacttcag
     6001 caggaagttg accgtttaga gcctttattt aagagtgaac aagaatataa aaatttcgta
     6061 aaagatcata accgtcatgt tgtgaaacgt ggagatcttg ctacatataa aggaaattgt
     6121 tacttaggga tcgatgccgg ttccactaca acaaaagttg ctttagctgg tgaggatgga
     6181 gaacttcttt acagctacta caataacaat aacggaagtc ctcttcacgc cgttgttgaa
     6241 gcgcttcatg agattgacgc acagatgcca aagacagcta agatcgtatc ttcctgttcc
     6301 acaggatacg gagaacatct tgtaaaagct gccttaaatc ttgactttgg agaagttgag
     6361 acgatcgcac attactatgc agctgcattc tttgatcctg atgtggactg tatccttgat
     6421 atcggtggtc aggatatgaa atgtatccgt atcaaaaatg gagttgttga tgatgtacag
     6481 ttaaacgaag catgttcttc tggatgcggt tcatttattg aaactttcgc aaaatcctta
     6541 aatcattctg tacaggaatt tgccaaagtt gctttaacag cacagaatcc gatcgatctt
     6601 ggttcacgtt gtacggtatt tatgaattct aaagtaaaac aggcacagaa ggaaggtgca
     6661 actgtcggag atatctctgc tggtcttgct tattctgtga tcaagaatgc tttatataaa
     6721 gttattaagt taacagaccc atctgacctt ggaaaacata tcgttgtaca gggtggtaca
     6781 ttttataatg atgcggttct tcgaagcttt gaacgtatct ccggatgtca tgctgtaaga
     6841 cctgatatcg caggtatcat gggtgccttt ggttccgcac tgattgcgag agaacgttac
     6901 gaagatggta tggaaacttc tatgcttcca ttagaagaaa tctttgcgat gagtgttgat
     6961 acttccatga cacgatgcaa aggctgcaca aatcactgcc tgttaacgat caataagttc
     7021 tctaacggac gccgttatat cactggtaac cgttgtgaac gtggtattgg gaaagaaccg
     7081 aatgctgaaa atattccaaa cctttacgat tacaaacttc atcgtacctt tgactacgaa
     7141 cctttatctg atgagaaagc cactcgtgga gtcattggta tccctagagt tttaaatatt
     7201 tatgagaact atccattctg gtatacattc tttacggatc ttggatttaa agttgtggta
     7261 tctcctgaat cttctcgtaa gatttatgag ttaggtatcg aatctattcc aagtgaatct
     7321 gaatgttacc ctgcaaaatt agcccatggt catgtgatgt ggctgatcaa acagggaatc
     7381 aaggatatct tctacccttg tattccttat gaacgtgacg aaatggaagg tacaaacaac
     7441 cattataact gcccgatcgt tacttcttat gcagaaaata tcaaaaataa catggaagaa
     7501 cttgcaacag agcatatcaa ctttatgaat ccattcttag cactggataa tgaagaagct
     7561 ttaaaatccc gtttatttga ggaattagaa gctcagtatc atttgactgc tgacgaaata
     7621 aatcacgcgg ttgataaagc ttatgcagaa ctttcacagg ttcgtacaga tatccagaat
     7681 aagggtgagg aagtccttgc ttaccttgct gaaactggca gaactggtat cgttctatgt
     7741 ggtcgtccat atcatatcga tcctgagatc aaccatggta ttccagaatt gatcaactct
     7801 tatggaattg ctgttttaac tgaggattct atttctcatt tatcaaaggt tgaacgtcca
     7861 ttaaacgtac aggatcagtg gatgtatcat tccagactat atgctgcagc aaactatgca
     7921 aaagcaaata aacagttaga agtcattcag ttaaactctt tcggttgcgg actggatgct
     7981 gttacaactg atacagtgaa agatatctta acaaaatccg gacgtatcta cacagtatta
     8041 aagattgatg aggttaataa ccttggagcg gcaagaatcc gtatccgctc actgatcagt
     8101 gctgtccgtg tccgtaaaaa acataagatc gaaccaaaac ctgtatctcc agcgatcaaa
     8161 cgtgtagaat ttacaaaaga gatgcgtaag aactatacac tgttagttcc tcagatgtct
     8221 ccgatccatt ttgagttctt agagcctgct ctgcagtctt gtggatacaa cgttgaagtt
     8281 ttaaatcagg gtggtatgga agatgttaac acaggtctta agtatgtaaa taatgatgcc
     8341 tgctatccat ctctgatcgt tatcggacag ttaatgaatg cattgctttc tggtaaatat
     8401 gatcctcata agatcgcact tgtcatctcc cagacaggtg gtggatgccg tgcaacaaac
     8461 tatatcagtt ttatccgccg tgcacttgaa aaagctggtt acggttatgt tcctgttgtt
     8521 ggtttaagtg cccagggaat cgagaaaaac agtggattta gctttacacc tggtttatta
     8581 aacaaagcag ttcaggcgat cgtttatggt gatgtcttta tgagaacggt ttatcataca
     8641 cgtccttatg aagtaaaacc tggttctgtc aacaaattac acagacaatg ggctgctaaa
     8701 tgtaaacgtg atattgccaa aggtaacttc tatacattcc agaagaatat ccgtggaatc
     8761 attcgtgact tcgaccgcat tccattaaag gatatcaaga aacctcgtgt cggaatcgtt
     8821 ggagagatcc ttgttaaatt ccttccagat gcaaataacc acttagtaga acttctggaa
     8881 cgtgagggtg cggaagctgt tgtacctgat ctgttagact tcttcatgta cagcttctac
     8941 aacagtaact tcaaataccg ttaccttggt aaatccaaga aatctgcgat cgtcagcaac
     9001 tccgcaatct ggatcgttga acgttatcgt cagactcttc gtaaagagct agccaagagt
     9061 aaccgttttg agccgcctgc atatatcaaa gaccttgcag aatatgcaaa accattcgta
     9121 tcgatcggta accagactgg tgagggttgg ttcttaacag gtgagatggt tgaactgatt
     9181 cacagcggtg ctccaaatat cgtatgtaca cagccatttg catgcttacc gaaccatgtc
     9241 gttggtaagg gtgttattaa agaaatccgt caggcattcc ctgatgcgaa catcgtggcg
     9301 atcgactatg atccgggagc tagtgaggtt aaccagatca accgtattaa gctgatgctt
     9361 tctgcggctg agaagaatat gaataaatag ttatttttct attgtttaga atcaaatgct
     9421 aataaaaaga agccatgttc cggcatagat aaatcttgtc tatgcttgta acatggcttc
     9481 ttgtttgttt ctttctattt caattagatc attctgctaa aatttcttcc agaatggctg
     9541 ttgcatcttc aatgcagcct ggacaggatc ttaagacttt tcccgtttca atccctttta
     9601 ggtccttaca ttttgttgct tgattcttct cctgaaagcg ttttacaagc tctcctgata
     9661 gtttataggt tgctgccttg gaaactggtt tctcgatgtc tccacagctt tttatggcac
     9721 cgatgactgc aacggctcct gtgatcgctc cacatgttcc ttccatgcag cccattccta
     9781 atccgtgacc ttccatcatc ttgaaaatga tggattcgtc gattcccatt tcttcagcaa
     9841 atgcacaggc aactgcctgg gcacagttgt agcctttctg gtggttttgt gctgctttgt
     9901 ctgttttgat actcatttgt aatgtcctcc aatttttatt cattattaat tagattgtag
     9961 atttcttctg ctgttttgtc tggatgttgt tttacttgat cgtagattgg tcttatcttg
    10021 gttgttgact cttctagttc atctgctatt tgttctagat tcttgttttt ctttactttt
    10081 ttctgaatca tagaaatttg atttagtaat tttcctgccc ttaccccacg attttccgca
    10141 ctctgtaact gactcactct gtcactcgct gccatctctt ctctcaaata tagatatctg
    10201 tcccgttcgt cctggctcat ctttaccatt tcttcttgta tcctttccat atatctattt
    10261 cctgctgatt cctgtgcaac ctcttcccat gtcgttgcgg cgatcaatct tgcccaacgg
    10321 tatagaggat gctttcttgc tttgcctttt gctgttttgg ttttctttaa ttctatcata
    10381 tggaattgaa attttctact gtagagttca ttggtttttt cgtctcgtag tgtcactttg
    10441 tggtaatatc caggactttt catcatgttg aaatttaaga ttcctacatg gatacaagga
    10501 gaaagcttcc agtaaggctc gcctttttta aaaccttctg tgaacatccg gcagttgtag
    10561 aaaaggcttc tctctgtcca gtcttcctga taggtatttt gcatttctat gttgatcttt
    10621 ttatttccgt taagagttaa cttgatatct agaattcctt ctttttcttc cttattttct
    10681 ccgcttgtaa atggatcttt gatttcta
//
LOCUS       DAOMUE010000034        10667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1498_length_10667_cov_2.774689, whole genome shotgun sequence.
ACCESSION   DAOMUE010000034 DAOMUE010000000
VERSION     DAOMUE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1498_length_10667_cov_2.774689"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..434)
                     /locus_tag="PLC71_02905"
     CDS             complement(186..434)
                     /locus_tag="PLC71_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRM01628.1"
                     /translation="MCQEQVEKALQQSGKRITQQRKILLDVILNGEWECCKEIYYEAV
                     KRDSSIGMATVYRMMTTLEEIGVIERRCVFRVRDEVEL"
     gene            756..1154
                     /locus_tag="PLC71_02910"
     CDS             756..1154
                     /locus_tag="PLC71_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRM01629.1"
                     /translation="MKIQESAENYLEAILILKQNKGSVRSIDIAHYLNFSKPSVSIAM
                     KNLRNSDMITVDSDGFISLTEAGHKIADTIFERHTVLSNMLIKLGVPAEIAAEDACRM
                     EHVISPESFHAIKAHLNSEEHIKALSNQNK"
     gene            complement(1251..1613)
                     /locus_tag="PLC71_02915"
     CDS             complement(1251..1613)
                     /locus_tag="PLC71_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRM01630.1"
                     /translation="MADIIIVLILIVIAVFAIKQTKKHMHGESPCCGGGSGTVKKVKE
                     KHLDGPVMGTKTMKIKGMTCEHCVNNVTNAINKVAGASAKVSLKDKEAIISYDRELSN
                     IELRKAVEDAGYEVISLS"
     gene            complement(1763..1936)
                     /locus_tag="PLC71_02920"
     CDS             complement(1763..1936)
                     /locus_tag="PLC71_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HRM01631.1"
                     /translation="MNPVSIIILLLIIAYCAFVIYKQVQKKKQGGCGGNCAGCSGCSS
                     FQVKDSSVKKKGR"
     gene            complement(2036..4222)
                     /gene="feoB"
                     /locus_tag="PLC71_02925"
     CDS             complement(2036..4222)
                     /gene="feoB"
                     /locus_tag="PLC71_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRM01632.1"
                     /translation="MAINIALAGNPNCGKTTMFNALTGANQYVGNWPGVTVEKKEGKL
                     KGKRKGEDITITDLPGIYSLSPYTLEEVVSRDFLLNENPDVIIDLVDATNIERNLYLT
                     TQLIETGVPVVIALNMADLLEKRGIKIDVERLSMLLNCPIVETSALKGKGLDEVVEEA
                     IKVAKKNTVDLPKEIFSKDVEAAIAEVKNVLPSSISEDKRRWYAVKFLENDSKVAESV
                     VLSGNGAKVVEDNRTKIEKAEDDDMESIVTDGRYQFIQKIVSTTVKKSGEKLTISDKI
                     DRIVTNRILGIPIFIAIMFVVYYISVTTIGTLVTDWTNDTFVAEMIQPAAQSFLEGIG
                     ASAAIQDLIVNGIIGGLGAVLGFVPQMAILFLFLSILEDCGYMVRIAFVMDRVFRHFG
                     LSGKSFIPLLISSGCGIPGIMASKTIEQDNDRRLTIMTATFIPCGAKLPVIAMMGGVM
                     TSYATGSYEAGGLMAPCMYFIGIVAVLVAAIILKKTKPFSGKPAPFVMELPQYHIPSA
                     KTVLLHVWERLKGFIIKAGTILFLACVVMWFLSGYGFVNGSFGAVEDPGNSLLAVIGG
                     AIAPIFAPLGFDNWKAVAASLSGFSAKESIVSTMGVLANVTGDASEDAVTVAEAVRTW
                     FPSAVAAFSFLLFNLLDSPCLAAISTMAKEMQSRKWFWFAILFQNIFAYVVTLIVYQI
                     GTFATGGAFTVGTAVGIVLLLVMLFLLFRPDPYKDQKVYSKRSVQA"
     gene            complement(4324..4548)
                     /locus_tag="PLC71_02930"
     CDS             complement(4324..4548)
                     /locus_tag="PLC71_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HRM01633.1"
                     /translation="MTLGDAAVGSTVIVAKIDGDGAYKRRIMDMGITKGSEIFIRKVA
                     PLGDPVEITVRGYELSVRKNDAQCVLVKEA"
     gene            complement(4660..4869)
                     /locus_tag="PLC71_02935"
     CDS             complement(4660..4869)
                     /locus_tag="PLC71_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRM01634.1"
                     /translation="MPLIMVGIGETCTIQRIGGSEETRRFLANLGFVQGAEVTVLSAI
                     GGNVIVNIKDSRVAINKDMARHIMV"
     gene            complement(5001..5288)
                     /locus_tag="PLC71_02940"
     CDS             complement(5001..5288)
                     /locus_tag="PLC71_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HRM01635.1"
                     /translation="MSIVIIGGHDRMVRQYETICKKHNCKAKIFTQMPSCLNKKIGNP
                     DLVVLFTNTVSHKMVRCAVSEAKNKNIEVVRSHSSSQAALTEILEQRCEIA"
     gene            complement(5390..5821)
                     /locus_tag="PLC71_02945"
     CDS             complement(5390..5821)
                     /locus_tag="PLC71_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HRM01636.1"
                     /translation="MSEVIVAFWTGTGNTAEMAEYVAEGIKEGGAEAKVVSVDDISAA
                     DLKDAKAFALGCPSMGVEQLEESTMEPFVEEVCGFASGKTIGLFGSYGWGDGEWMRNW
                     VEQMEGAGATVVGGEDAICQEAPDEDAQDELKAMGKQLAAI"
     gene            complement(5876..6487)
                     /locus_tag="PLC71_02950"
     CDS             complement(5876..6487)
                     /locus_tag="PLC71_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HRM01637.1"
                     /translation="MRYQVELSHVKWKKCMQMQMAMHCAPVLAGLKPSNAVTLDYIDS
                     KELIQSLAGSDIKCGLIYSGNGKCLWLLYREQQVNQYLMDPENQRFLKHCGYSSFQIQ
                     NILYTLKNRYRLYKAGQADFPHELGLILGYPLCDVIGFIKNNGQNCLYCSYWKVYDNL
                     SQTKKIFEVYDKVRFQMVKLVEYGKSFKELVDMYTNYQVPRYA"
     gene            complement(6673..8406)
                     /gene="dnaK"
                     /locus_tag="PLC71_02955"
     CDS             complement(6673..8406)
                     /gene="dnaK"
                     /locus_tag="PLC71_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRM01638.1"
                     /translation="MSKVIGIDLGTTNSCVAVMEGGVPVVISNAEGGRTTPSIVAFAK
                     NGERLVGDPAKRQAVTNVSRTVASVKRQMGTDRKVKIDGKKYTPEEISAMILAKLKKD
                     AESYLGEEVTRAVITVPAYFSDAQRQATKNAGKIAGLSVERIINEPTSAALAYGLDKS
                     VSQKIMVYDLGGGTFDVSVIEIGDGVVEVLATAGNNHLGGDDFDQRIMNYLIGEFKKA
                     EGIDLSKDQTAVQRIKEEAEKAKKELSSSMTVQINLPFIAVGKDGPKHMDITLTRSKF
                     EELTSDLIDQTAGPVHQALSDAGISTSELHMVLLVGGSTRMPAVSEKVRQLTGKEPSK
                     NMNPDECVALGAAIQGGKIAGEAGLNEILLMDVTPLSLSIETAGGVATRLIDRNSTIP
                     TRYSQIFTTAANFQTTVEIKVLQGERAMARDNQVLGTFKLKGIKRAMRGVPQIEVTFD
                     IDVNGILNVSAKDLATGKEQSITITASSNMSDKDIERAMEDAKAYESEDQEKRDAIGS
                     YNEGENMLYKGEAYLAQHKKELSREQRSELKSAISDLKHSMKRIKPQNITEEQGRAIK
                     EASERVNQLIR"
     gene            complement(8441..9190)
                     /locus_tag="PLC71_02960"
     CDS             complement(8441..9190)
                     /locus_tag="PLC71_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRM01639.1"
                     /translation="MSDPYEILGVQRGASEEEIKKAYKRLSRKYHPDANLDNPKAAEE
                     KFKELQQAYQQVMKEKTTGYSQSSYGGYQSQGNPYGGQGNPYGNQQGNPYGEWNPFED
                     IFGQFSGYYTNGGAQQRQKTTTGYEKDTHLRAAGNYVRNGYYQEARNVLDGMEPARRN
                     ARWYYYSAAANQGLGNNVTAMEHAKQAVSMEPDNYEYQMLLQQLQGNGSWYQQRQSTY
                     QNPYSTGGDWCMKMCMLNLMCNCCLGGRYWC"
     gene            complement(9183..10028)
                     /locus_tag="PLC71_02965"
     CDS             complement(9183..10028)
                     /locus_tag="PLC71_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HRM01640.1"
                     /translation="MFGYIIVNKSEMKFREFDVYHGYYCGLCRKLKEHYGKFGQITLS
                     YDMVFVLMTLTSLYELETTKSMKRCVTHPLQKHEERVNNITDYVAHMNILLTYYKCKD
                     DWNDDRKLEKLVLEKILYHKSGFSRNFYREKWNKINDILEKLSEEEKKDNQDIDQMSG
                     MFGKVMAEIMLYQDDEWKELLNQFGFFLGKFIYLMDAYEDIEDDLKNHNYNPLKNIYT
                     KPEFEDMIHQILTMMMAECSKAFEQLPLIDDIDILRNVLYSGVWYRYEQVREKREKEK
                     EEKNV"
     gene            complement(10030..10455)
                     /locus_tag="PLC71_02970"
     CDS             complement(10030..10455)
                     /locus_tag="PLC71_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01641.1"
                     /translation="MRDKFNQFMQGRYGNDDLNRFLMKIILAAFVLSLFTGRIVFGGV
                     VSLSRIFYWIALALLIYCYIRMFSRNIYKRSEENRWFLNKTSNMRGLWSNRKYKAQQM
                     KNYHIYKCPGCGQKIRIPRGKGKIEVRCPKCRTTFVKRS"
BASE COUNT         2871 a         2350 c         1627 g         3819 t
ORIGIN      
        1 ttataatatc catacagctt ataggcgcta atgcaaaata ctgcaataca gagcaagaga
       61 aagatatgat atagtatctt tttcataacc acgattctcc cttttctttt actataacag
      121 aaaaagtatc tgatttcatt gattgaaacc aaatactttc ttaatttttt atgattgatt
      181 taattttaaa gctctacttc atctcttact cgaaatacgc atcttctctc gatgactccg
      241 atctcttcga gtgttgtcat catgcggtat actgttgcca ttccaatgga agaatctcgt
      301 ttgactgctt cgtaatagat ctctttgcag cattcccatt ctccattcaa gataacatct
      361 agtagaatct ttctctgttg tgtgattctc ttaccacttt gttgtaaagc cttctctact
      421 tgttcttggc acatgctgtt ctccaccttg ccttgctgtt gtatgcatga ctgcgggata
      481 aagtctatgg catgacactt tatctcgcat tcatactaca taaatgatta tactatataa
      541 aggtcgtttt gatgagttag tctcatctaa ttacatatat aaggttagca ttacctaacc
      601 attttgtcaa tagtttttct taattttttt attttttcac ggtatttttt ctcatgtgct
      661 tccattgaaa atacacactg tttatgatac aatgataacc aaaataaaca tatcttgtag
      721 actagatata caattattag taaggaggaa tctttttgaa aatacaagaa tccgctgaaa
      781 attatttgga agctatttta attttgaaac aaaacaaagg gtctgtaaga tccattgata
      841 ttgctcatta tttgaatttc tctaaaccaa gtgtcagtat tgctatgaaa aatcttcgta
      901 attctgatat gatcaccgtt gattctgatg gtttcatctc tttaactgaa gctggtcata
      961 agattgctga tacgattttt gagcgccata ctgttttatc taacatgctg atcaaacttg
     1021 gagttcctgc agagattgca gctgaagatg cctgccgtat ggaacatgtg atcagccctg
     1081 aaagctttca tgcgatcaaa gcacatctga attctgaaga acatattaaa gctctcagca
     1141 atcaaaacaa ataatttgaa acaaaagcac ttttttctgt atattacaga tcttaaaagt
     1201 gcttttttgt tttctactgg tttcgataac ttcttctaca acaccgttct ttatgaaagt
     1261 gatatgactt catatcctgc atcttctact gcttttctaa gctcaatatt acttaattca
     1321 cgatcatatg aaataattgc ttctttatct tttaaagata cttttgctga agctcctgca
     1381 actttattga tcgcatttgt cacattgttg acacagtgtt cacaagtcat acctttgatc
     1441 ttcattgttt tcgttcccat cacaggacca tctaaatgtt tttcttttac tttctttaca
     1501 gtacccgatc cgccgccaca gcaaggactt tccccatgca tatgtttctt tgtctgtttg
     1561 atcgcaaaaa ctgcgataac aataaggatg agtacaataa taatatctgc cattttatga
     1621 tacctctttt tactttacat tatttattta gtaatttacg gatcagttta taaagctggt
     1681 atccgatcac cccgataaga tataatccaa taaagattgc aaatgcataa aacatagaac
     1741 ctgttgaatt tcccattgct gattaccttc cttttttctt gactgatgaa tccttcacct
     1801 gaaaactgct gcatcctgag cagcctgcac agttgcctcc acatccgcct tgtttcttct
     1861 tctgcacctg tttataaata acaaatgcgc aatatgcaat aatcaataac aatatgataa
     1921 tacttactgg attcataatc gtccctcctt tatatcatat cttttttata tgaaatttga
     1981 taagcaagaa gccggatgtt tacacatcca gcccctctat tatataccat ttcgactacg
     2041 cctgaacaga tctctttgaa tatactttct gatctttgta tggatctggt ctgaatagca
     2101 ggaataacat tacaagcaat aacacgatac ctactgctgt tcctactgta aatgctccac
     2161 ctgttgcaaa tgtaccgatc tgatatacga tcagtgttac aacataagca aaaatattct
     2221 ggaaaagaat cgcaaaccag aaccattttc tagactgcat ttcttttgcc attgtagaga
     2281 ttgctgctaa acatggagag tctaacaggt taaacagtaa gaatgaaaat gctgctacag
     2341 cacttgggaa ccaagttctt actgcttctg ctactgtgac agcatcttct gatgcatctc
     2401 cagttacgtt tgcaagtaca cccattgttg atacgataga ttcttttgcg gagaatcctg
     2461 ataaagaagc tgcaacagct ttccagttgt caaatccaag tggtgcaaag attggtgcga
     2521 tcgctccacc gataactgct aataaactgt ttcctggatc ttcaacagct ccgaatgaac
     2581 cattgacgaa tccatatcca cttaagaacc acataacaac acaagctaag aacaggattg
     2641 ttccggcttt gatgatgaat ccttttaatc tttcccatac atgaagtaat actgtcttag
     2701 cagatgggat atgatactgt ggaagttcca ttacgaatgg agctggcttt ccagagaatg
     2761 gttttgtctt ctttaagatg attgctgcaa ctaatacggc tacgatacca atgaagtaca
     2821 tacatggtgc cattaatcca cctgcttcat aacttcctgt tgcataactt gtcataacac
     2881 cacccatcat agcgataact ggaagtttcg ctccacatgg aatgaatgtt gctgtcataa
     2941 ttgtcagtcg tctgtcgttg tcttgttcga tcgtcttaga tgccatgata ccaggaattc
     3001 cacatcctga agaaatcagt aatggaatga aggatttacc tgataatccg aagtggcgga
     3061 atacacggtc cattacgaac gcaatacgta ccatatatcc acagtcttct aagatagaca
     3121 ggaataagaa taagattgcc atctgaggaa cgaatccaag gacagctcct aaaccaccaa
     3181 tgataccatt aacgatcaga tcctggattg ctgcgcttgc accgattcct tctaagaagc
     3241 tttgagcggc tggctggatc atctcagcta caaatgtatc atttgtccag tctgttacta
     3301 atgtaccgat cgttgttaca gaaatatagt atacaacaaa catgatcgcg ataaagattg
     3361 ggatacctaa gatacggttt gtcacaatac ggtcgatctt atcagagatc gttaattttt
     3421 ctccgctctt cttcactgta gtggaaacga tcttctgaat gaactgataa cgtccatctg
     3481 ttacgatact ttccatatca tcgtcttcag ctttttcaat ctttgttctg ttatcttcta
     3541 ctacttttgc accatttcct gaaagtacta cgctttctgc tactttgctg tcattttcca
     3601 ggaattttac tgcataccat cttctcttat cttcagaaat agatgatgga agtacatttt
     3661 taacttcagc gattgctgcc tctacgtctt tagagaaaat ctcttttggc agatctacgg
     3721 tattcttctt cgcaactttg attgcttctt ctactacttc atcaagacct tttcctttta
     3781 atgcggatgt ctcaacgatt ggacagttta ataacataga aagacgttcc acatcaattt
     3841 tgatcccacg tttttctaat aaatctgcca tatttaaagc gatgacaact ggtacaccag
     3901 tttcgatcag ctgtgttgtc agatataagt ttctctcgat gttagttgca tcaactaagt
     3961 cgatgataac atctggattt tcatttagta agaagtctcg gctgactact tcttctaatg
     4021 tatatggtga aagagaatag atacctggaa ggtcagtgat cgtgatgtct tccccttttc
     4081 tctttccttt taatttacct tctttttttt ctacggtaac acctggccag ttaccaacat
     4141 actggttagc tcctgtcagg gcattgaaca tggtagtttt accacagttc gggttacccg
     4201 caagtgcgat attaatagcc atttcttttt tacctccctg gggaattgct tccctcttaa
     4261 ataatagctt ttcttgatat tagatattac taacttttat tagatactac taactcttat
     4321 gtattaagct tctttaacta atacacactg agcatcattc tttcttacag aaagttcgta
     4381 tcctctgact gtaatttcaa caggatctcc aaggggagct actttacgaa tgaaaatttc
     4441 gcttcctttt gtgattccca tatccataat acgacgtttg taagcaccgt ctccatcgat
     4501 ctttgcaact ataactgtgc ttccaactgc tgcgtctcct aaagtcatgg tcatatctcc
     4561 tttcttctca atatttcatt gtcttcaaat ctatcaagca tctatcaaat aaaccgctac
     4621 gtgacaattt attcctataa acgcaatata acaaacatat taaaccataa tatgtctagc
     4681 catatcctta ttaattgcaa ctctggagtc tttaatgttc acaatgacat ttcctccaat
     4741 tgctgataat acagttactt ctgctccctg tacaaaaccg aggtttgcta agaatcttct
     4801 tgtttcttca cttccaccga ttctctgaat tgtacatgtt tctccaattc caaccataat
     4861 caaaggcatg atatcttccc ctttttctct attattgtta gttgtaacta tcttttgtta
     4921 gtgtaaacta atttcctttt acaatttgtt agtatattct aacttcaaaa tactgtcaag
     4981 gggtttttta tattttttcg ttatgcaatt tcacaacgtt gctctaaaat ttctgttaat
     5041 gcagcctgac tgcttgagtg acttcttact acttcaatat ttttattctt tgcttcactg
     5101 acagcacaac gtaccatttt gtgtgaaact gtgttcgtaa ataatacgac taaatccggg
     5161 tttccaattt tcttattcag acatgatggc atctgtgtaa atatctttgc cttgcagtta
     5221 tgcttcttac agattgtttc atactgacgt accatgcggt catgtcctcc aataattaca
     5281 atactcattt tgtgttccct ccttctagat attgtcttgc tgtttttaat acagccggac
     5341 atatgatcca tacgcccggc tgtttatttt cttattcttt atttataaat tagattgctg
     5401 ccagctgttt acccatggct tttaattcat cctgagcatc ttcatctgga gcttcctgac
     5461 agattgcgtc ttctccgcca acaacagttg ctcctgctcc ttccatctgc tctacccagt
     5521 ttctcatcca ttctccgtct ccccatccgt aagatccgaa taatccgatt gtttttcctg
     5581 aagcgaatcc gcagacttct tcaacaaatg gttccattgt tgattcttct aactgttcaa
     5641 ctcccataga tgggcatcca agtgcaaatg cttttgcatc ttttaaatct gctgcgctga
     5701 tatcgtctac agaaacaact tttgcctcag ctccgccttc tttgattccc tctgcaacat
     5761 attctgccat ttctgctgta tttcctgttc ctgtccaaaa tgcaacgatt acttcgctca
     5821 tgacttatcc tcctaatgat tgtctttctt ttttgtttga atgttttatt tttagttaag
     5881 catacctagg tacctgataa tttgtgtaca tgtcgacgag ttctttaaaa ctctttccgt
     5941 attccacaag tttgaccatc tggaaacgaa ctttatcata aacttcaaag atctttttcg
     6001 tctgagaaag gttgtcgtaa accttccagt agctgcaata taaacaattt tgtccattgt
     6061 ttttgataaa tccgatcaca tcacataatg gatatccgag aattaagcca agttcatgtg
     6121 gaaaatctgc ctgccctgct ttgtaaagcc tgtaacgatt cttaagtgta taaaggatat
     6181 tttgaatctg aaaggatgaa tagccgcagt gttttaaaaa tctttggttt tctggatcca
     6241 tcagatactg gttaacttgc tgttcacgat acagcagcca gagacattta ccatttcctg
     6301 aatagatcag tccacacttg atatcacttc ccgcaagtga ctgaattaat tcttttgagt
     6361 caatatagtc gagagttact gcattggatg gctttagccc cgcaagtaca ggtgcacaat
     6421 gcatcgccat ctgcatctgc atacattttt tccattttac atgacttaat tctacttgat
     6481 atctcattga ttcctcctta ggttagtctt aactaactat ttgcatcata cacctataca
     6541 atagatttgt ctatagtaaa aaatgagaag atttatcact atattttctg cttttatagt
     6601 cattaatctt cccattttta tatcttttat tctattttca tatccattaa tacaatcggt
     6661 ccaaacactt tcctatcgta tcaactgatt tacacgttcc gatgcttctt tgatcgctct
     6721 gccttgttct tctgtaatat tttgtggttt gatccgtttc atcgaatgtt tcagatcact
     6781 aattgctgat ttcaactctg atctttgttc tctggataat tcttttttat gctgggctaa
     6841 atatgcctca cccttataaa gcatattttc tccctcatta taacttccga tcgcatcacg
     6901 tttttcctga tcttcggatt catatgcctt agcatcttcc attgctcgtt ctatgtcttt
     6961 atctgacatg ttagaacttg ctgtgatcgt aatggactgt tcttttcctg tcgcaagatc
     7021 tttggcagac acattcagaa ttccattgac atcgatatca aatgttactt cgatctgtgg
     7081 aaccccacgc atggctctct tgatcccttt taatttaaaa gttccaagga cctgattgtc
     7141 ccttgccatc gcacgctctc cctgcaaaac tttgatttct acagttgtct ggaaatttgc
     7201 tgctgtcgta aaaatctgac tgtatcttgt tgggattgta gaatttcggt cgatcaatct
     7261 agttgcaacg cctccagctg tctcaatgga taaggacaac ggtgtaacat ccattaataa
     7321 aatctcattt aaccctgctt ctcctgcgat ctttccaccc tgaattgctg cacccagagc
     7381 tacacattca tctggattca tattcttgct tggttctttg cctgttaact gtcttacttt
     7441 ctctgaaact gctggcattc tggtagaacc gccgactaac agtaccatat gaagttcgct
     7501 ggtactgatt cctgcatcag ataacgcctg atgaactggt cctgctgtct gatcgatcaa
     7561 atctgaagtc agttcttcaa atttggatct ggttaatgtg atatccatat gtttagggcc
     7621 atctttccct actgcgataa atggcagatt aatctgtaca gtcatagaag aagataactc
     7681 tttctttgct ttttctgcct cttctttgat tctctgaaca gctgtctgat ccttggacag
     7741 atcgatccct tctgcttttt taaattcccc gatcagataa ttcatgatcc tttgatcaaa
     7801 atcatctcct ccaagatgat tatttccagc cgttgccagt acttcgacaa ctccatcacc
     7861 aatctcgatc accgaaacgt caaaagttcc tccaccaaga tcatatacca taatcttctg
     7921 ggatacggat ttgtctagtc catacgccaa tgcagcagat gtcggttcgt taatgatacg
     7981 ttccacactt aatcccgcaa tctttccagc attctttgta gcctgtctct gagcatcgct
     8041 aaaataagct ggtactgtga tcacggctct cgttacttct tccccgagat agctttctgc
     8101 atcctttttt aatttggcca ggatcatcgc tgaaatttct tctggtgtat atttcttccc
     8161 atcaattttt actttacgat ctgttcccat ctgacgtttt acggatgcaa ctgtacgact
     8221 cacattcgtt accgcctgtc gttttgctgg atctccaacc agtcgttctc catttttcgc
     8281 aaatgccact attgatggcg tggttcttcc accttctgca tttgagatca caactggtac
     8341 tccaccttcc atcactgcta cacaagaatt tgttgttccc aaatctattc ctattacttt
     8401 gctcatagtc ttccctccta aatcagtgtt gttttacaga tcaacaccag tatctgccac
     8461 cgagacaaca gttgcacatc aaatttaaca tgcacatctt catacaccag tctccacctg
     8521 tggaatatgg attctgataa gtactttgtc tttgctgata ccaagaacca tttccttgca
     8581 gctgttgtaa taacatctga tattcataat tatcaggttc catggaaact gcctgttttg
     8641 catgttccat agcagttaca ttatttccaa ggccttgatt ggcagctgca ctgtaatagt
     8701 accatctcgc atttcttctg gctggttcca tgccatctaa aacattcctt gcttcctgat
     8761 aataaccatt ccttacataa ttacctgctg ctcttaaatg cgtgtccttc tcatatcctg
     8821 tcgttgtctt ctgtctttgc tgagctccgc catttgtgta ataaccacta aattgtccaa
     8881 aaatatcctc aaatggattc cattctccat acggatttcc ttgttgattt ccatatggat
     8941 tcccttgtcc accataagga ttcccctgac tctggtatcc accataagaa ctttggctgt
     9001 atccggttgt tttctccttc ataacctgtt gataagcttg ctggagctct ttaaatttct
     9061 cctcagctgc ctttggatta tcaagattgg catctggatg gtattttctg cttaatcttt
     9121 tatatgcttt ttttatttct tcttctgaag cacctctttg cactcctaga atttcatatg
     9181 gatcagacat tcttttcttc cttttctttt tctcgtttct ctcgtacctg ttcgtatcga
     9241 taccatactc cggaatataa gacatttctc aatatatcta tatcatcgat cagtggaagc
     9301 tgttcaaaag ctttcgaaca ctctgccatc atcatcgtta gaatctgatg gatcatatct
     9361 tcaaattccg gttttgtata tatatttttc agtggattat agttgtgatt cttcagatca
     9421 tcttcaatat cttcatatgc atccatcaaa taaatgaatt taccaaggaa aaatccaaat
     9481 tgattcagaa gttctttcca ctcatcatcc tgatacaaca tgatctctgc catgaccttt
     9541 ccaaacatcc ctgacatctg gtcgatatcc tgattgtctt ttttctcttc ttctgacagc
     9601 ttttctaata tatcattgat cttattccat ttttccctat aaaaatttct ggaaaaccca
     9661 cttttatggt ataaaatttt ttctaatacc aacttctcta atttgcggtc atcattccaa
     9721 tcatctttac atttgtaata agttaacaaa atattcatgt gtgcgacata atccgtgata
     9781 ttgtttactc tttcctcatg tttttgcaat ggatgtgtga cacatcgctt catactcttt
     9841 gttgtttcta attcataaag actggttaag gtcatcaata caaagaccat atcataggaa
     9901 agtgtgatct gaccaaactt tccataatgt tcttttaatt ttctgcacaa accacaatag
     9961 tatccgtgat agacatcgaa ttcgcggaat ttcatctctg atttgttgac aatgatatac
    10021 ccaaacattt tagctcctct ttacgaatgt ggtacgacac tttggacaac gaacttctat
    10081 ctttcctttt cctcttggaa ttctgatctt ctgtccacat cctggacatt tgtaaatatg
    10141 gtagttcttc atctgctgtg ctttgtattt tctattgctc cagagtcccc gcatattgga
    10201 tgttttattc aggaaccatc tgttttcttc ggatcgttta tagatgtttc ttgaaaacat
    10261 acggatatag caataaatca aaagtgctaa tgcaatccaa taaaaaatac ggctaagtga
    10321 tactacgccg ccaaatacga ttcttcctgt aaatagtgat agtacaaatg ctgctaaaat
    10381 aatcttcatt aaaaagcggt tcagatcgtc atttccgtat ctgccctgca tgaattggtt
    10441 gaatttatct ctcatagact tctccttctt tgatatctgt tctattatgg atatcacatt
    10501 taagtataat ggtaattata cgcaaaccta tgcagaatag caatgaattg gttgtaaaga
    10561 aattataaat tttattttat aattctaaga tatcaaaaac cattctcatc atgctcgtga
    10621 atataattga catttataat tatttttgtt aatatattcc atgtaaa
//
LOCUS       DAOMUE010000035        10341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1573_length_10341_cov_2.166051, whole genome shotgun sequence.
ACCESSION   DAOMUE010000035 DAOMUE010000000
VERSION     DAOMUE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10341
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1573_length_10341_cov_2.166051"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLC71_02975"
     CDS             complement(<1..447)
                     /locus_tag="PLC71_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HRM01642.1"
                     /translation="MILKELLERSEYTLVQGSEDVEISTLVYDSRKIEKGSVFVCISG
                     AAFDGHKFVAQAAELGAAAVVVEKDVEVPENLTVIKFDNTRLALAEMSAAYFGYPAEE
                     LTTIGITGTKGKTTTTYMIRAVLEHAGQKTGLIGTIETIIGDERIPS"
     gene            complement(483..1334)
                     /gene="dapF"
                     /locus_tag="PLC71_02980"
     CDS             complement(483..1334)
                     /gene="dapF"
                     /locus_tag="PLC71_02980"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRM01643.1"
                     /translation="MKFTKMHGCGNDYVYVNCLEEKINNPNKVAKFISDRHFGIGSDG
                     MICICPSDKADFRMAMYNSDGSEGAMCGNGVRCIAKYVYDYGLTDKTTITIETKGGIK
                     ELDLTVEDGKVTWVKVDMEAPITKASQIPAIYDDLDEVIDQPVIVDGKEYNITCISMG
                     NPHGVVFVDSVADIDIEKIGPKFENHPMFPDRVNTEFIELVDDKTIKMRVWERGAGET
                     LACGTGACASAYASYLNKKTGKKVLVHLLGGDLQIEYDEEKDTIFMTGPATTVFDGEI
                     DLTGIDD"
     gene            complement(1525..2796)
                     /locus_tag="PLC71_02985"
     CDS             complement(1525..2796)
                     /locus_tag="PLC71_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM01644.1"
                     /translation="MESFREMSKEELQAQEVEVNKKYQEFKDLHLSLNMARGKPAPDQ
                     IDHANGMLETMTDYHAKDGLDVRNYGVLDGLPEMKEIFSDLLDIPAKNIIVGGNASLN
                     LMFDQMMRLVVFGTAGEKPWGQLEKVKFLCPAPGYDRHFSVTETLGFELITVDMTSEG
                     PDMDQVEKLVAEDESIKGIWCVPKYSNPQGICYSDETVDRLASMKTAAKDFRIFWDNA
                     YGVHLIFEDVKVKNILDACKEAGNDNRAYYFFSTSKITFPGAGVSIIASSDDNIAEIK
                     KVMTAQTIGYDKINQLRHVRFFGGIVGVRKHMQILADCLKPKFETVLNTLEKELKGSG
                     LITWENPKGGYFVSVDVLPGCAKRVVELAKEAGVALTGAGATYPYKNDPKDSNIRLAP
                     SYPTVEELQQAMDLFCVCVKKAGIEKLLEQA"
     gene            complement(2859..3869)
                     /locus_tag="PLC71_02990"
     CDS             complement(2859..3869)
                     /locus_tag="PLC71_02990"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRM01645.1"
                     /translation="MKKVSVIGAGSWGSALAVLLANNGHEVTLWTHDPHEIEMLSTKR
                     EQVEKLPGVKLPDNIMIEADLKTALTDEDVVVMAVPSPVVRMVAKQMSPFIKDGQIIV
                     NVAKGIEDVTYKTLSDIIEEEIPNAEVCVLSGPSHAEEVGRGIPTTVVVGAKNKETAE
                     MLQDVFMNKVFRVYTSSDIVGIELGGALKNVIALAAGTVDGLGYGDNTKAALMTRGIA
                     ELTRLGEALGGKPETFSGLTGVGDLIVTCTSVHSRNRKAGYLMGKGMTAEEAMKEVKM
                     VVEGVYSAKAALGLAKKCNVAVPIVEAVNRVLFENADPKEEVSNLLLRERKQEHTNLE
                     WN"
     gene            complement(3885..4508)
                     /gene="plsY"
                     /locus_tag="PLC71_02995"
     CDS             complement(3885..4508)
                     /gene="plsY"
                     /locus_tag="PLC71_02995"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRM01646.1"
                     /translation="MAVCIVVCMIVGYLFGCIQTGYIVGRVNKIDIRDYGSGNSGTTN
                     TLRTLGKTAAIITFLGDAVKGLVAVNLARYVIMPAFHVEGEAYLWLLTGFAVVLGHNF
                     PFYLGFKGGKGIATTGGVIFAFHWQLGLVCAAVFLIITAISKYVSLGSICMVLIIPFI
                     LYFNNPHDWKLVIVGCIFTAMAIWRHRANIGRLMNGTENKLGQKVKR"
     gene            complement(4508..5830)
                     /gene="der"
                     /locus_tag="PLC71_03000"
     CDS             complement(4508..5830)
                     /gene="der"
                     /locus_tag="PLC71_03000"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRM01647.1"
                     /translation="MSKPIIAIVGRPNVGKSTLFNALAGEKISIVKDTPGVTRDRIYA
                     DVTWLNYQFTLIDTGGIEPDSGDLLLSHMRGQAEIAMETADVIIFLTDVRQGLVDADY
                     QVADMLRRSGKPIVLAVNKVDNYEKFVLDTYEFYNLGLGTPFPVSANSKIGFGDLLDE
                     VLVHCNPQDADEKEDERPRVAIIGKPNAGKSSLINKLLGEDRLIVSDIAGTTRDAIDT
                     TVKRNGKEYVFIDTAGLRKKARVKEDIERYSVIRTVAAVERCDVAILVIDAEEGITEQ
                     DAKIAGIAHERGKGMIIAVNKWDLIEKNDKTIYKFTNQVREVLSYMSYAELVFISAKT
                     GQRLPKIFDVLDMVIENHALRVQTGVLNEILTEAVAMKQPPSDKGKRLKLYYITQVSV
                     KPPTFVVFINDRQLMHFSYTRYLENQIRNTFGFRGTPIHIIARERKER"
     gene            complement(5947..6765)
                     /gene="pgeF"
                     /locus_tag="PLC71_03005"
     CDS             complement(5947..6765)
                     /gene="pgeF"
                     /locus_tag="PLC71_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRM01648.1"
                     /translation="MHFKKSNDNQSTILHEGSVPYLTFKKLDQAGVRHGFSTRMGGVS
                     EGMFSTLNLSYNRGDKKEAVDENFRRISEAIGFDHTKLVFSNQIHETRIHKVTKEDCG
                     KVMKDMDGLATNEQGIPLYTGYADCVPLFFYDPVEKAAALAHSGWRGTVGRIGAKMVQ
                     FLENEYGSKKENIIAAIGPSICRSCYEVSEDVADEFKKELTGHDAKEFLDDKGNGKYQ
                     LDLWKANEIILTEAGIRPENLDITDICTCCNPDLLFSHRASHGKRGNLAAFIVL"
     gene            complement(6755..7102)
                     /locus_tag="PLC71_03010"
     CDS             complement(6755..7102)
                     /locus_tag="PLC71_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRM01649.1"
                     /translation="MKKNNRETGAKAEAIACWFLKQQGYDVLEQNFYTKVGEIDIIAK
                     EDQTLVFVEVKYRKDDKKGYPAQAVDQRKQQKIRKSAMIYLKKNHLSFEQPIRFDVVE
                     ILGKKIRVIKHAF"
     gene            complement(7125..7781)
                     /locus_tag="PLC71_03015"
     CDS             complement(7125..7781)
                     /locus_tag="PLC71_03015"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRM01650.1"
                     /translation="MTKKEEREARKLEKLRLEKERMYEMFSFEREYGDHQAICGIDEV
                     GRGPFAGPVVAAAVILPKDCDILYLNDSKKLSEKKRELLYDEIYEKAVAIGIGMSSEE
                     VIDEINILQATYKAMQQAISKLSIKPDLLLNDAVTIPDVEIEQIPIIKGDAKSASIAA
                     ASIVAKVTRDRMMKEYDIIYPGYDFAKNKGYGTKAHIEGIKKQGICDIHRRTFVKKYI
                     "
     gene            complement(7799..8320)
                     /gene="lepB"
                     /locus_tag="PLC71_03020"
     CDS             complement(7799..8320)
                     /gene="lepB"
                     /locus_tag="PLC71_03020"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRM01651.1"
                     /translation="MFKSILRLLGTILIAVAFAFVLIMCVGERTKVSGHSMEPHLHDH
                     DQVLLDKLTYRFRDPKRYEIIVFPGPEGGDQFFVKRVIALPGETVKITKGKVYVNDKE
                     VKDYSKDHTTDSCELKGKFHLSSDEYFVLGDNRDNSNDSRYKEVGPVKRSKITGRIIF
                     RFYPWKNAGVIRP"
     gene            complement(8346..>8443)
                     /locus_tag="PLC71_03025"
     CDS             complement(8346..>8443)
                     /locus_tag="PLC71_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HRM01652.1"
                     /translation="EKASKIVLEEFRNGKLGKISLETPEEGTKEA"
     assembly_gap    8444..8453
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8454..9190)
                     /gene="ylqF"
                     /locus_tag="PLC71_03030"
                     /pseudo
     CDS             complement(8454..9190)
                     /gene="ylqF"
                     /locus_tag="PLC71_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756911.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
     assembly_gap    9277..9286
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9287..9756)
                     /gene="lepB"
                     /locus_tag="PLC71_03035"
     CDS             complement(<9287..9756)
                     /gene="lepB"
                     /locus_tag="PLC71_03035"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRM01653.1"
                     /translation="MRKGYTVTYQMRKKQRQQREIVIQYVLKFVGVVLLAFLLVRFVC
                     FSFTIQGDSMSPTIERGEKHLVNRLIYQIKGPSRYDVVIFKSDDKNGNYYVKRVIGLP
                     GEKVQIKNGRIYVNGKKTKAFSSQKILSAGLAADEITLKSKQYFVVGDNYNNSE"
     gene            complement(9864..10211)
                     /gene="rplS"
                     /locus_tag="PLC71_03040"
     CDS             complement(9864..10211)
                     /gene="rplS"
                     /locus_tag="PLC71_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRM01654.1"
                     /translation="MSAQDIIKSIEDAQLREVAEFKTGDTVRVHAKVKEGNRERIQVF
                     EGTVIKRQNGGARETFTVRKQASGVGVEKTWPVHSPIIEKIEVVRRGKVRRAKLYYLR
                     DRVGKAAKVKERV"
BASE COUNT         2699 a         2433 c         1484 g         3705 t
ORIGIN      
        1 ggatggaatt ctctcatctc cgatgattgt ctcaatcgtt ccgatcaatc ctgttttctg
       61 tcctgcatgt tctaggactg cacggatcat atacgttgtt gttgtctttc ctttggttcc
      121 tgtgataccg atcgttgtca gttcttctgc tggatatcca aagtatgctg ctgacatctc
      181 ggctaaagca agtcttgtat tatcaaactt gatcactgtc agattttctg gtacttctac
      241 atctttttct actacgactg ctgccgctcc aagttcagca gcctgtgcta caaatttatg
      301 accatcaaag gctgctccgc tgatacatac aaatacactg cctttttcaa tctttcttga
      361 atcatatact aatgtagaaa tctctacatc ttcacttcct tgcacgaggg tatattctga
      421 acgttctaat aattctttta atatcatgtt ctatctccaa taattcttct atctttgtac
      481 tcttaatcgt caattcctgt aagatcgatc tcgccatcaa acactgttgt tgctggtcct
      541 gtcataaaga tcgtatcttt ttcttcatca tattcaatct gaagatctcc acctaaaaga
      601 tgtaccaaga ctttctttcc tgttttctta ttcagataag atgcataagc acttgcacat
      661 gctcctgttc cgcaggcaag agtttctcct gcacctctct cccatactcg catcttgatc
      721 gttttatcat caacaagttc aataaattct gtattcacac gatctgggaa cattggatga
      781 ttttcaaact ttggtccgat cttctcaatg tcgatatctg caacagaatc tacaaataca
      841 actccatgtg gatttcccat agaaatacat gtgatattat attcttttcc atcaacgatc
      901 actggctgat cgatcacttc atcaagatca tcatagattg caggaatctg tgaagctttt
      961 gtgattggtg cttccatatc aactttcacc catgtcactt tgccgtcttc aactgttagg
     1021 tctaattctt taattccgcc ttttgtctcg atcgtgatcg ttgttttatc tgtcagtcca
     1081 taatcataga catattttgc aatacagcgg acaccatttc cgcacatggc tccttctgaa
     1141 ccatcagagt tatacatggc cattctaaaa tcagctttat ctgatggaca gatacagatc
     1201 attccatcag aaccaattcc aaaatgccgg tcactgataa attttgccac tttatttggg
     1261 ttattgatct tttcttctaa acagtttaca tatacgtagt cattgccaca accgtgcatc
     1321 tttgtaaatt tcatgtataa tcaactcctt tttattattt tacgaataaa cagttttatt
     1381 ttaccacatc aacgtgttaa ttgtatagat acaatctttg aatttatatt ttaaaaacca
     1441 aatactagta ttacaaaaac aaatgaggaa catgccttat caccattgat aaagaatatt
     1501 cctcatagta ttttttatga actcttatgc ctgttctaac agtttttcaa ttcctgcttt
     1561 cttcacacat acacaaaaca gatccattgc ctgctgtaac tcttctactg ttgggtaaga
     1621 aggtgccagt ctgatattgc tgtcttttgg atcattctta tatggatatg tagctcctgc
     1681 tcctgttaat gcaacacctg cttctttggc aagttctaca actctctttg cacatcctgg
     1741 aaggacgtct acagatacaa aatatccacc ttttggattc tcccatgtga tcaatccgct
     1801 tccttttaac tctttctcta acgtattaag aactgtctcg aatttaggtt ttaaacaatc
     1861 tgctaagatc tgcatatgtt ttctgactcc tacgataccg ccgaagaaac gtacatggcg
     1921 aagctgattg atcttgtcat atccgatcgt ctgggctgtc atcactttct tgatctctgc
     1981 aatattatca tcgcttgatg caatgataga tactcctgca cctgggaatg tgatcttgga
     2041 tgttgagaag aaataatatg ctctgttatc atttcctgct tctttacatg catctaagat
     2101 attcttaact tttacatctt caaagattag atgtactcca tatgcattat cccagaagat
     2161 tctgaaatct ttggctgctg tcttcataga agctaaacga tcaaccgttt catcactata
     2221 gcagattccc tgtggattgg aatattttgg cacacaccag attcctttga tactttcatc
     2281 ttccgcaacc agtttctcta cctgatccat atctggacct tcagatgtca tatcgactgt
     2341 gatcagttca aatcctaaag tttctgtaac tgaaaaatgt ctgtcatatc caggagctgg
     2401 acataagaat tttactttct ctaactgtcc ccaaggcttc tcacctgctg ttccaaatac
     2461 aaccagtctc atcatctgat caaacatcag attcagactc gcatttcctc caacaatgat
     2521 attctttgct ggaatatcta ataaatcgct gaatatttct ttcatctctg gaagtccatc
     2581 aagaactccg taattacgca catccagacc atcttttgca tgataatctg tcattgtctc
     2641 aagcatgcca tttgcatgat cgatctgatc tggtgctggt tttcctcttg ccatgtttag
     2701 tgaaagatga agatctttaa attcctgata ctttttgttc acttccactt cctgtgcctg
     2761 taactcttct tttgacattt ccctaaatga ttccattgtt gtcttccttc cttcatgttt
     2821 ttatgttttg tatatattta tgtttttata tatcctgatt aattccattc aagatttgta
     2881 tgttcttgtt ttctctcacg aaggagtaag tttgaaactt cttcttttgg atccgcattc
     2941 tcaaataaca ctctgttcac tgcttccaca attggtacag ctacattgca tttctttgca
     3001 agtccaaggg ctgctttcgc tgaatatacc ccttctacaa ccatttttac ttctttcata
     3061 gcttcctctg cagtcatacc ttttcccatt aagtatcctg ctttacgatt tcgactatgt
     3121 acgcttgtac atgtaacgat cagatctcca actcctgtaa ggcctgaaaa tgtctctggt
     3181 tttccaccaa gtgcttctcc aagacgtgtc agttctgcga ttcctcttgt cattaatgct
     3241 gcttttgtat tatctccata tccaaggcca tcaaccgttc ctgctgctaa tgcaataacg
     3301 ttctttaatg ctcctccaag ctcaattcca acaatgtcag aacttgtgta tacacggaat
     3361 actttattca taaatacatc ctgaagcatt tccgctgtct ctttattctt tgcaccaact
     3421 acaaccgttg taggaattcc tcttcctact tcttctgcat gactaggccc tgataatacg
     3481 caaacttctg catttggaat ctcttcttca atgatatccg ataatgtctt atatgtaaca
     3541 tcttcgatac cttttgcaac gttaacaatg atctgcccgt cttttataaa tggggacatc
     3601 tgttttgcaa ccatacgaac aacaggtgaa ggaactgcca taacaacaac atcttcatct
     3661 gttaaagctg ttttaagatc tgcctcgatc ataatattat ctggcaattt tactccaggt
     3721 aatttctcta cctgctctct ctttgtggaa agcatctcta tttcatgcgg atcatgtgtc
     3781 cacaatgtca cttcatgtcc attatttgct aataataccg caagggcact tccccagctt
     3841 cctgcaccga taacgctaac tttcttcatc tgtttaagtt cctcctaacg ttttactttc
     3901 tgacctaatt tattctcagt accattcatc aaacgcccaa tattcgcacg atggcgccag
     3961 attgccatgg ctgtaaatat acatccaaca ataactaatt tccaatcatg tggattgtta
     4021 aaatacaaaa taaatggaat gatcagcacc atgcagatcg atcctaacga tacatactta
     4081 gaaatagccg taatgatcaa aaatactgct gcacagacca acccaagctg ccagtgaaat
     4141 gcaaaaataa ctcccccagt tgtcgctatt ccctttccac ctttaaaccc aagataaaac
     4201 gggaaattat gtcccagtac tactgcaaat cctgtcaaaa gccaaagata agcttctcct
     4261 tctacatgaa aagccggcat gatcacatat ctcgcaagat tcaccgccac aagtcctttg
     4321 acggcatctc caagaaatgt aatgatcgct gctgtctttc ccaaagttct taatgtattc
     4381 gttgtcccgg aatttccact tccgtaatct ctgatatcga tcttatttac tcgtccgaca
     4441 atatatcctg tctgaataca cccaaacagg tatccgacga tcatacacac aacaatacaa
     4501 actgccatta tctttccttc ctttccctcg caataatatg aattggtgtt cctctgaatc
     4561 caaatgtatt acggatctgg ttctcaagat atcttgtata tgaaaaatgc attaactgac
     4621 gatcattgat aaacacaaca aatgttggcg gttttacaga aacctgtgta atatagtaaa
     4681 gttttaatct ctttcctttg tctgatggtg gctgtttcat cgctaccgcc tctgtaagga
     4741 tctcgtttaa gactcctgtc tgtacacgta atgcatgatt ctcgatcacc atatcaagaa
     4801 catcaaaaat cttaggaagt ctctgtcctg tcttagcaga gatgaaaaca agttctgcat
     4861 atgacatata agataagact tctctaacct ggtttgtaaa cttgtagatt gtcttatcgt
     4921 tcttctcgat gagatcccat ttgttaaccg caatgatcat accttttcca cgttcatggg
     4981 caattcctgc gatcttagca tcctgttctg tgattccttc ttccgcatca atgactaaga
     5041 ttgccacatc acaacgttct actgctgcaa ccgtacggat cacactgtaa cgttcgatat
     5101 cttctttgac tctggctttc tttcgaagtc cggctgtatc gatgaataca tattcttttc
     5161 cattacgttt gaccgttgta tcaatcgcat ctcttgttgt tcctgcaata tcagaaacga
     5221 tcagacggtc ttctcccaat aatttattga tcaatgaaga ttttcctgca tttggctttc
     5281 cgatgattgc aactcttggt ctttcatctt ctttctcatc tgcatcctgt ggattgcagt
     5341 gaacaagaac ttcatctaac agatcaccga atccaatctt agaattcgct gaaactggaa
     5401 atggtgttcc aagccccaga ttgtaaaatt cataagtatc caaaacgaat ttttcgtaat
     5461 tatctacttt attgactgct aacacaattg gttttcctga tcgtcttaac atatctgcca
     5521 cctgatagtc tgcatcgaca agcccctgtc ttacgtctgt aaggaagatg atcacatctg
     5581 ctgtctccat tgcgatctct gcctgtcctc tcatgtggct taataataga tcaccactgt
     5641 ctggttcaat accacctgta tcaatcaatg taaactgata gtttaaccat gtcacatctg
     5701 catagatacg gtctcttgta actcctggtg tatctttaac aattgaaatt ttttctcctg
     5761 ccagtgcatt aaacaatgta gactttccta cattcggtct tcctacaatg gcaatgatcg
     5821 gcttactcat aatctatata cctctctaaa tgtttcaaaa cctgtactgt ttcttagtat
     5881 accttatctt atgaaattcg tccagtgtga agaatttcac aagatatatt ctttttattt
     5941 acctttttat aacacaataa atgctgccag attaccacgt ttcccatgac ttgctctatg
     6001 cgaaaacagt agatcaggat tacaacatgt gcagatatct gtaatatcaa gattttctgg
     6061 acggattcct gcttctgtca aaataatctc atttgctttc cagagatcaa gctggtattt
     6121 tccattccct ttatcatcca aaaattcttt tgcatcatgt cctgtcagtt cttttttaaa
     6181 ctcatctgca acatcttcac taacctcgta gcaacttcgg cagattgatg gtccaatggc
     6241 tgcgatgata ttttcctttt tacttccata ttcattttct aaaaattgta ccatcttggc
     6301 accgattctt ccaacagtgc ctctccatcc tgaatgtgct aaagctgcag ccttttctac
     6361 cggatcataa aaaaacaaag gcacacaatc tgcatatcct gtataaagcg gaatcccctg
     6421 ctcatttgta gcaagaccat ccatatcttt cataactttt ccacaatctt ctttcgttac
     6481 cttatggatt ctcgtctcat gaatctgatt tgaaaatacc agctttgtat gatcgaatcc
     6541 gatcgcttct gagattcttc tgaaattttc atccacagct tcttttttat ctccacggtt
     6601 atagcttaaa ttcaacgtgg aaaacattcc ttcgctgact ccacccatac gtgttgaaaa
     6661 tccatgtctg actcctgcct gatccaattt tttaaatgtc agatatggaa cagacccctc
     6721 atgtaatatc gtagactgat tatcgtttga ttttttaaaa tgcatgtttt atcaccctga
     6781 tcttctttcc taaaatctcc actacatcaa aacggatcgg ctgttcaaaa gatagatgat
     6841 tcttctttaa ataaatcatt gcactctttc ggatcttctg ctgttttctt tggtctacag
     6901 cttgtgccgg atatcccttt ttgtcatctt ttctgtattt tacttccaca aataccaatg
     6961 tctgatcttc ttttgctata atatcaatct ctccgacttt tgtataaaag ttttgttcaa
     7021 ggacgtcata tccttgctgt ttcagaaacc aacaggcaat cgcttctgct tttgcccctg
     7081 tctccctgtt atttttcttc atattatata tacttttttg ttttttatat gtatttcttt
     7141 acaaatgttc ttctatggat atcacagatt ccctgctttt tgattccttc aatatgtgct
     7201 ttggttccat atcctttatt ttttgcaaag tcatatcccg gataaattat atcatattct
     7261 ttcatcatac ggtcccttgt aactttcgca acaatacttg ccgctgcgat cgatgcactt
     7321 tttgcatctc ctttgatgat cggaatctgt tcgatctcaa catctggaat tgtgaccgca
     7381 tcattcaata aaagatctgg ttttatcgaa agctttgaaa ttgcctgctg cattgcctta
     7441 taagttgcct gcaggatatt gatctcatcg atcacttctt ctgaagacat tccaataccg
     7501 attgcaactg ctttctcata aatctcatca tataaaagct cccttttctt ctctgaaagc
     7561 tttttggaat cattcagata taaaatatca cagtcttttg gcaaaatcac tgcagccgca
     7621 acaacaggtc ctgcaaatgg tcctcttcca acttcatcaa ttccacaaat cgcctgatga
     7681 tctccatatt ctctttcaaa agaaaacatc tcatacattc tttctttctc aagccttaat
     7741 ttttctaact tcctggcttc tcgttcttct ttctttgtca taagtcttaa aacccttcct
     7801 atggccggat cacacctgca ttcttccatg gataaaaccg gaaaataatc ctccctgtga
     7861 tctttgacct ctttactgga ccaacctctt tgtatctgct gtcattacta ttatctctgt
     7921 tatctcctaa aacaaaatat tcatctgatg aaagatgaaa ctttcctttt aattcacatg
     7981 aatctgtcgt atgatctttg gaataatctt ttacttcttt gtcatttaca tagacttttc
     8041 cttttgtgat ctttaccgtt tctcctggaa gtgcgatcac tcgctttaca aaaaactgat
     8101 cgccaccttc tggtcctgga aatacaataa tctcatatcg tttaggatcc cgaaaacgat
     8161 atgttaactt atctaacaac acttgatcat gatcatgcaa gtgcggttcc attgaatgtc
     8221 cacttacttt cgtccgttca ccaacacaca taataagaac aaatgcaaat gcaaccgcga
     8281 tcaaaatggt acccagcaac cttagaatac ttttaaacat tcttatatct gcccctcaaa
     8341 ggcttttatg cctcttttgt cccttcttct ggtgtttcca gagaaatttt tcctaatttc
     8401 ccatttcgga attcctctaa tacgatcttt gatgcttttt cgtnnnnnnn nnncaattac
     8461 gatttagcgc aatctgttct aagattttca ctttatcttc attttcatct acttgatagc
     8521 gatcagcaag aattccctga taatgatttt ttaaatactc gatcagttcg atcgccattt
     8581 catcctgatt gaggatttca tctctgatcg atccgatcaa tgccagacga agcccaacct
     8641 gctggtcttc aaatttaggc cataaaattc caggggtatc taataattca atattcttat
     8701 ttaatttgat ccactgtttt ccttttgtaa ctcctggttt atttcctgtt tttgtacagg
     8761 cttttccaac cagactgttg ataaatgttg attttccaac gtttgggatt cctgcgatca
     8821 ttgctctgat cggacggttt ttaatcccac gttttctatc tctctcaagt ttttccttgc
     8881 aggcatccat cacgatcgcc tgtgtacctt tgacaccttt tcctgatctt gcatttgttc
     8941 ttgctacata gaatcctttt tctttaaaat attcttccca ctgtgcagtc acacgttcat
     9001 ctgccatatc tgtcttattt aacaagatca atcgtgactt gttcgctgcc aactggtcaa
     9061 tatccggatt tctgctgctt agtggaattc ttgcatccac aagttcgatc ataatatcga
     9121 tcagtttcat gttttcctgc atcatacgtt ttgctttggt catatgaccc gggtaccact
     9181 gaaaatccat tctttatttc tccttccctc tgtttaccac ggccagtatt tgataccgac
     9241 ctttccaatg atatttgttc ttttgatatt tccaacnnnn nnnnnntctt cactattatt
     9301 atagttatct cccacaacaa agtactgctt actctttaat gtaatctcat ctgctgctaa
     9361 tcctgcagat aaaatcttct gacttgaaaa tgcttttgtt ttctttccgt ttacatagat
     9421 tctgccgttt ttgatctgaa ccttttctcc cggaagacct atcactcgtt ttacatagta
     9481 atttccattc ttgtcatctg atttaaatat gaccacatca tacctgcttg gtcccttgat
     9541 ctgatagatc aggcgattca ccagatgttt ttcacctctt tcaatcgttg gtgacataga
     9601 atcaccttga atcgtaaaac tgaaacacac aaatcttact aacaaaaatg ccagtaacac
     9661 aactccgaca aattttaaga catactgaat cacgatttcc ctttgctgac gctgtttctt
     9721 gcgcatctga taggttactg tgtatccttt cctcatagaa gaactccttt cctcttcgta
     9781 tagaaagatt cgtaaaattc ctgtatacta aaacagggat aagctataca gcctatccct
     9841 ctcttttgac aatctttgtc tgattatact ctttctttta cttttgctgc tttaccaaca
     9901 cgatcacgta agtagtataa tttagcacgt cttactttac cacgtcttac tacttcaatc
     9961 ttttcgatga ttggagaatg tactggccat gttttttcaa caccaacacc acttgcctgt
    10021 tttcttacag taaatgtttc gcgtgctcca ccattctgtc ttttgatgac tgttccttcg
    10081 aaaacctgaa ttctttcacg gtttccttct tttactttcg cgtgaactct tacagtgtct
    10141 cctgttttga attcagcaac ttctcttaac tgtgcatctt cgatgctttt aataatatcc
    10201 tgtgcgctca ttgttgttcc tccttgaacg tttttagatg ttcttaatac caaatgtaac
    10261 agaggaccat ccatatttat aacaacttga acatattatc atattttttt atgtgaagca
    10321 agtgtttttt tgtatttttt a
//
LOCUS       DAOMUE010000036        10222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1602_length_10222_cov_2.686732, whole genome shotgun sequence.
ACCESSION   DAOMUE010000036 DAOMUE010000000
VERSION     DAOMUE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10222
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1602_length_10222_cov_2.686732"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PLC71_03045"
     CDS             complement(<1..632)
                     /locus_tag="PLC71_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRM01655.1"
                     /translation="MKYVTKIILIMTCLIIMLPFGGCGRKKQITAQKAGVLKPEAPGK
                     EVLDHGEAVVDISNVAQGYVALRYKGSAKKISVEVIGKNNKVYKYFIERTEEPTYFPL
                     TSGNGTYQISVYENVQDDEYSVLMMDSFEVKLKNKFLPFLYPNQYVEFTSKTKAVKEA
                     KKLAKDSKDDLAIVKAVYNYVVKNVKYDNEKAENVQSGYLPSVDETLKTK"
     gene            complement(696..1562)
                     /locus_tag="PLC71_03050"
     CDS             complement(696..1562)
                     /locus_tag="PLC71_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM01656.1"
                     /translation="MEIQQLYYYMELCKQKNFTEAGYACNMTQGALSKQIRKLENELG
                     ITLIRRNTRKFELSKEGEIFLSYAKKMTGTYEEMLKNVQKNQEIKIGCMPVLAPYHFA
                     RLVADFRKEYPDIKLVIDERIASEIQENSDRYDFLILRENMMEDQKKFRFSPLYDDKL
                     CAVLYEKHPLYGRDRLQLKELKDDVFIFPERGSGSYEVFYKSCEKAGFEPKIAFEFPQ
                     ANTIMSFVSEGVGVTITFSTVYREAKCAGVKMIPLEDELHSVISLFYRKNKPLDYAKK
                     QFLNYVREHLYT"
     gene            1724..2383
                     /locus_tag="PLC71_03055"
     CDS             1724..2383
                     /locus_tag="PLC71_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HRM01657.1"
                     /translation="MKNKKRFFYHRLLFYILGLLIMSVGSNLFLKAALGVAPSCTMAL
                     TLSYLSPIHSYALFNFLINSTFLVCEALILHEFGKMQLTQLGLTFVYSLFIEFTSYGL
                     FFIEPHGLAAKILLSVIACAVLSIGVGFTISSGFAVMPMEGLVKTISDKKNISFGAVR
                     VCLEVLFTVCSAISSFVLLPELPSVGIGTVIAAFLSGNITNIFSSLFHKRINAYLGFQ
                     A"
     gene            complement(2475..3380)
                     /locus_tag="PLC71_03060"
     CDS             complement(2475..3380)
                     /locus_tag="PLC71_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRM01658.1"
                     /translation="MRKLSRRAASIALAAAMLFTTAGVSQKKVEAASTGKLSVTGYQD
                     YNDAQKILKEVNKYRKKNGRKALKMDRGLTNSAIMRGFETTIYIPETSPHRRPNGKLS
                     KSINKKIIYENCAQSAGTTPKQIVKGWINSSTHRKGLLLSNAKSVGVAAVTVVSRDGY
                     EANTWVLDFSSSKAKKVEKSKARKQFTKNIVTKNSYLKKSYLKLDSRYGTIWETEKMQ
                     MKPTYQGKMMKEYGVYPTVINTSSFTSWKSSNPSVASVDSNGRITGNKAGTATISVKL
                     KTGTKITISKKIKVVPTNQQIDPWE"
     gene            complement(3417..4049)
                     /gene="nth"
                     /locus_tag="PLC71_03065"
     CDS             complement(3417..4049)
                     /gene="nth"
                     /locus_tag="PLC71_03065"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRM01659.1"
                     /translation="MNKKELALEVIERLKNEYPDADCTLEYDQAWKLLVSVRLAAQCT
                     DARVNVVVKGLFEKYPTVEALAAADVADIEEIVRPCGLGKSKARDISKCMKVLRDEYN
                     GKIPTDFKSILSLPGVGRKSANLIMGDVFGKPAIVTDTHCIRLVNRIGLVDGIKDPKK
                     VEMALWEIIPPEEGSDFCHRLVWHGRDVCTARTKPHCERCCLNDICAQII"
     gene            4157..4885
                     /locus_tag="PLC71_03070"
     CDS             4157..4885
                     /locus_tag="PLC71_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01660.1"
                     /translation="MKKSFLKGASIFLASVLTVGMCSAYSVNAKAKFSLPKKTTLSYD
                     KKDNGGNGFYTNVIYIKGTNRSNSESILRNGMKVKSSNRKVISTKTFGKDYYLIADNN
                     QVGIGVKLKKTGSTKITAKFKYRGKTYSVKTTVKVIKYSNPFKKIVVDGRDCTSDFKN
                     KNGSSISFMGRNIKITPNKNWRVKSIYTYKPGATKMKKVKGSSVTIPTNCRAFITMEN
                     KKNKLTEKVTFDEMDTSEDPGFIF"
     gene            complement(4982..6337)
                     /locus_tag="PLC71_03075"
     CDS             complement(4982..6337)
                     /locus_tag="PLC71_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM01661.1"
                     /translation="MEITDSARNRMFSNKDLVRLIIPLIIEQMLTVTVGLADSIMTAN
                     VGEEAVSGVSLVDSVMVLMINLFSALATGGAVVAGQFLGQRKNIMACKTADQLILFIV
                     SLSVVIMAGIYACKNFILNVVFGNIEPGVMHNAEIYLMIVTASIPFIAIYNGCAALFR
                     IMGNSKISMNMSMLMNGINIAGNAILIYGFKFGVEGVAIPTLVSRIVAAVVMVRLLAK
                     QNQDVHLSKHIHFHFKGYLIQKILHIGVPNGIENSMFQLGRIMVLSIVAGFGTSSITA
                     NAIGNVVASFELIPGIGVGFAVLTVVSRCIGAGDYEAARYYTKKLLKVAYAALIALNI
                     IVVMILPLIIQVYHLSPETAAITRKILLYHTLCCVTIWPPAFTIPNTLRAANDVKYCM
                     VVASATMWIFRIFCSVVLGKWMGLGVFGVWVAMTLDWVFRGILFVKRYIQGKWQRMAL
                     I"
     gene            complement(6528..7133)
                     /locus_tag="PLC71_03080"
     CDS             complement(6528..7133)
                     /locus_tag="PLC71_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRM01662.1"
                     /translation="MKKNTIICISREFGSGGREVGQQLSKKLGIPFYDKEILARVAEE
                     SDMSEEFVKNHEENAVYSHIINVPIGHRFPGVDTEEIMTPEKLFMIQAHVIHELAAEN
                     ESCIFVGRCADVVLKDHENCYSFFIHSPLEERIKRIVETEHVTERQAKKMIKQTDWER
                     SSYHNYYTKQKWGDPGNYDLMINSARTGIQKAVEIIMDYVK"
     gene            complement(7213..8163)
                     /locus_tag="PLC71_03085"
     CDS             complement(7213..8163)
                     /locus_tag="PLC71_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRM01663.1"
                     /translation="MGNKDITDFNEAQLRKRAASKEELNICLTNNYAFQKIFKNEEIV
                     KGFLIALLHLKETDIEELEVTDPFVEGEVKEEKEGILDVKLILNGGKKINIEMQNTYQ
                     EDWAERSLFYNCRMFTEGLKKGESYYELPPCIHVGILDFNYLKSPGFYHRILLKDDTT
                     GELYSSKFQFHVIELSKIATTKGKARKQDLYKWAKLISASTWEEIREESEGNHYMEKV
                     RDEMIKMSQDESERYLYLREQMAIRDKASQLRSAENIGIRKGELLKLVTLVQRKIEKG
                     DTVDKIADDLLEDQEIIEKIYNLIRKYPDKDEKEICNFLI"
     gene            complement(8249..8437)
                     /locus_tag="PLC71_03090"
     CDS             complement(8249..8437)
                     /locus_tag="PLC71_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6472 family protein"
                     /protein_id="HRM01664.1"
                     /translation="MAKKKSAGCEYCSNYVYDEDYEYYVCDINLDEDEMARFMSYSDW
                     DCPYFQMDDEYAIVRKQN"
     gene            complement(8449..9144)
                     /locus_tag="PLC71_03095"
     CDS             complement(8449..9144)
                     /locus_tag="PLC71_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM01665.1"
                     /translation="MDLNKLKEIQAVVFDMDGLMFDSERYVQKSWDIAGERLGYGPLG
                     HNIVNTLGTNLTNRKKYFLEHYGNDFPFDKFLDGYRDAYYELVEDGVPAKKGLHEILK
                     VLREKGLKIGVATSSSEEHAVSNLKREGIFDYFDSVITGNMIEHGKPEPDIYIEACRQ
                     LKVNPSKAIALEDAINGIRSAHGAGMNPVMIPDIVQDTSKVDDILFGKCESLLEFAEI
                     LQGVTLDIEETQK"
     gene            9270..>10222
                     /locus_tag="PLC71_03100"
     CDS             9270..>10222
                     /locus_tag="PLC71_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HRM01666.1"
                     /translation="MKSSSNLKSLLKNIDRKGYPAYKNTQGTYDFGTYFLSIDHVQGD
                     PFASPSKLSIHVKGRSAGFPASFYDTKYKQVALCDHLTRLFYQTLSKISFRAKGSGKS
                     GLFSVSKCGQQVLERTACTINPSNGDISVHFEAGFPANGRTVNARELDKMLFGLLPDC
                     VSSSLFYKNIDQKMLESVIYLSEDQHTIRKKLSSMGLVAFIADGSVLPRESGISQKPL
                     RNCVKFQSPDTYRVSFELPHQGMITGMGIPKGITLIVGGGYHGKSTLLKALELGVYDH
                     VKGDGREFVITDPTAMKIRAEDGRSITNTDISMFINNLPNG"
BASE COUNT         2836 a         2147 c         1507 g         3732 t
ORIGIN      
        1 ttctttgtct tcaatgtctc atctaccgat ggcagatatc cactctgaac attctctgct
       61 ttctcattat catattttac attcttgacc acataattat aaacagcttt gacgatcgcc
      121 agatcatctt tggaatcttt tgccagtttc ttcgcttcct ttactgcttt cgtcttactt
      181 gtaaattcta catactggtt aggatataaa aatggcagaa atttattttt taatttcacc
      241 tcaaaactat ccatcatcaa aacactgtat tcatcgtcct gcacattttc atatacagag
      301 atctgatatg taccatttcc actggtcaat ggaaaatatg tcggctcttc tgtccgttca
      361 ataaaatatt tatatacttt attattcttt ccgatcactt caacactgat cttctttgca
      421 cttcccttat atcgcagtgc tacatagcct tgagccacat tcgatatatc aaccacagcc
      481 tctccatgat ccaaaacttc cttccccggt gcttctggct ttaatactcc tgctttctga
      541 gctgttatct gcttcttccg tccacaccca ccgaatggta acataatgat caagcatgtc
      601 atgatcagta ttatttttgt gacatacttc ataagaattc tccttatatt tactttcttt
      661 agtatagcag attcacaata tgcctgctaa ttcacttacg tatacaaatg ctcccttaca
      721 taattcaaaa actgcttctt tgcataatcc aatggcttat tttttctata aaatagtgat
      781 atcacagaat gtaattcatc ttctagtggt atcattttaa ctccagcaca tttagcttct
      841 ctataaaccg ttgaaaatgt gatcgtaacc ccaacccctt cacttacaaa actcatgatt
      901 gtatttgctt gtggaaattc aaaagctatt ttcggttcaa atcctgcttt ctcacaactc
      961 ttataaaaca cttcgtaact tccgctgcct ctctctggga agataaaaac atcatctttc
     1021 aattctttca gttgaagtcg atctcttcca taaagcggat gtttttcata taacactgca
     1081 cacaatttat catcatacag tggggaaaaa cggaattttt tctgatcttc catcatattt
     1141 tctctcaaga tcaagaaatc ataacgatcg gagttttcct gaatctcact tgcgattctt
     1201 tcatcgatca caagtttgat atctggatat tcttttctga aatccgccac caatcttgca
     1261 aaatgatatg gtgcaagaac cggcatacaa ccaatcttaa tttcctgatt tttctgaaca
     1321 tttttcagca tctcttcata ggttcctgtc attttctttg cataagataa aaagatctct
     1381 ccttctttgg acaactcaaa tttccttgta ttcctgcgga tcagtgtaat tcctaactca
     1441 ttttccaact tacggatctg ttttgataat gcaccttgtg tcatgttaca ggcatatcca
     1501 gcttctgtga aatttttctg tttacataat tccatataat aatataattg ctgtatttcc
     1561 atttttccat acccttttca aatatcttcg tatattccta tttggaatat atcaaatctc
     1621 aactggaatt gcaatacttc gctctctcta tatataataa gaaacacaaa agggaatacg
     1681 attgataaaa tatgaaattt tgatgaaagg aatcgttgat attatgaaaa acaaaaaaag
     1741 atttttttac catagattgt tattttatat tttaggactg ttgatcatgt ctgttggttc
     1801 taatttattt ttgaaagcag ctcttggagt tgcacctagc tgtacgatgg ctttgacatt
     1861 aagttatctt tccccaattc acagctatgc cttatttaac ttcctgatca actctacttt
     1921 cttagtttgc gaagctctga tccttcatga attcgggaaa atgcagttga ctcagcttgg
     1981 tttgactttt gtttattctt tatttatcga atttacttct tatggtttat tctttattga
     2041 acctcatgga cttgccgcta agattttact atctgtcatc gcatgtgccg ttctatcaat
     2101 aggtgtcgga ttcacgatca gttccgggtt cgctgttatg ccaatggaag gacttgtgaa
     2161 aactatctct gataagaaaa atataagctt tggcgctgtc agagtttgtc tggaagtttt
     2221 atttactgta tgttctgcga tctcttcttt tgtattgctt ccagaattac catccgttgg
     2281 aatcggaacc gtgatcgccg ctttcttaag tggtaatatt acaaatatat tttcttctct
     2341 atttcataag agaatcaatg cctaccttgg atttcaggca tgatcctata catgatccta
     2401 taaaaaaacc gtaatctcaa atgaacctct tgggattacg gttttttatt tcaccctgat
     2461 tctttagttt ttgcttattc ccatggatca atttgctgat tcgtcggtac gactttgatc
     2521 ttctttgaaa tcgttatctt tgtccctgtt tttaatttta cagaaatcgt tgctgttcca
     2581 gctttatttc ctgtgattct tccattcgag tcaacagatg ccacggatgg attactactc
     2641 ttccatgatg tgaaagatga tgtattgatc actgttggat acacaccgta ctctttcatc
     2701 attttgcctt gatatgttgg cttcatctgc attttttctg tttcccatat tgtaccgtat
     2761 ctgctgtcta gttttaaata actcttcttt aaatatgaat tctttgtcac gatatttttt
     2821 gtaaattgct ttcttgcctt ggatttttct actttctttg ctttggaact gctaaagtct
     2881 aatacccagg tattggcttc gtatccgtct cttgacacaa ccgtaactgc tgcaacccct
     2941 acactttttg cattcgaaag taacagacct tttctatgtg tggaagaatt gatccaaccc
     3001 tttacgatct gcttcggtgt agttcctgcg gattgtgcgc agttttcata gatgatcttc
     3061 ttgttgatac tttttgaaag ctttccattt ggtcttctat gcggacttgt ttctggaata
     3121 tagatcgttg tttcaaatcc acgcataatt gctgaatttg tcaatcctcg atccatcttc
     3181 aatgctttgc gtccattttt cttacggtat ttgttcactt cttttaagat tttctgtgca
     3241 tcgttataat cctgatatcc tgtaacagat aattttcctg tagaagcagc ctctaccttc
     3301 ttctggctga ctcctgcagt tgtaaatagc attgccgctg ctaatgcaat actggcagct
     3361 cttcttgata gtttcctcat aaactttctc ctttcatact tttgatcatg gtacctttat
     3421 ataatttgtg cgcagatatc attcaaacaa catctctcac aatgaggctt tgttcttgcc
     3481 gtacagacat cccttccatg ccataccagc ctgtggcaga aatcacttcc ttcttctggt
     3541 ggaataatct cccataatgc catctcaact tttttagggt cttttatccc atcaaccaat
     3601 ccaatccggt tgaccaagcg gatacaatgt gtatccgtaa cgatcgcagg tttgccaaaa
     3661 acatctccca tgatcaggtt ggcacttttt cttccgaccc caggaagact taggattgat
     3721 ttaaaatcgg ttgggatctt accgttatat tcatctctta aaactttcat acatttgctg
     3781 atgtctcttg ccttgctctt tccaagacca cagggccgta cgatttcttc aatatctgca
     3841 acgtctgctg ctgccagtgc ttctactgtt ggatattttt caaataatcc ttttaccacg
     3901 acattcaccc gtgcatccgt acactgagcc gccagtctta cgctaactaa taatttccac
     3961 gcctgatcat attccagtgt gcagtctgca tctggatatt cattcttcag acgctcaatg
     4021 acttctagtg ccagttcttt tttattcata acagtatcct ccttgaaggt tgttttatcc
     4081 ttattatacc ttacattttt gcatatttct atgctataat gattttgtat tttttgttca
     4141 tcgaaaggag aattttatga agaaaagttt tttaaaaggt gcctctattt ttctggcatc
     4201 tgtattaaca gtaggtatgt gttctgctta tagtgtaaat gcgaaagcaa aattttctct
     4261 acctaaaaag acaacattaa gttatgacaa aaaagataat ggcgggaatg gtttttacac
     4321 aaatgtgatc tatatcaagg gaacaaatcg ctctaattca gaatccatcc tgcgtaatgg
     4381 tatgaaggtt aaatcttcaa atagaaaggt tatttctaca aaaacttttg gaaaagatta
     4441 ttatcttatt gcagataaca atcaagtagg aatcggtgta aaacttaaga aaacaggatc
     4501 aacaaaaatc actgcaaaat ttaaatacag aggtaaaact tattctgtaa aaacaacagt
     4561 caaagttata aaatactcaa atccttttaa gaagatcgta gtggatggaa gagactgtac
     4621 ttctgatttt aagaataaaa acggatcttc gatctccttt atgggacgta acatcaagat
     4681 cactcctaat aagaactgga gagtaaaatc tatttatact tacaagccag gagctactaa
     4741 aatgaaaaaa gtaaaaggtt cttccgttac aatcccaaca aattgccgtg cttttattac
     4801 aatggaaaat aaaaagaata aattaacgga aaaagttact tttgatgaaa tggatacttc
     4861 tgaagatcct ggatttatat tctagacaca aattttaaaa catcccagaa tcgtcttttt
     4921 atacctgacg attctgggat gttcttatct tctacaattt ctttatttga tctgtttcat
     4981 gttaaatcaa tgccattctc tgccatttcc cttgtatgta tcgtttgaca aacaggattc
     5041 ctctgaatac ccagtctaat gtcattgcta cccagactcc gaagactcca agtcccatcc
     5101 attttccaag aacaacgctg cagaagattc taaatatcca catcgtggct gatgctacaa
     5161 ccatacagta tttgacatca tttgcggctc ttaatgtgtt cgggatcgtg aatgctggcg
     5221 gccagatcgt tacacagcat aaagtatgat ataataagat ctttcttgtg atcgctgctg
     5281 tttctggtga taaatgatat acctgaatga tcagtggtaa gatcataact acgatgatat
     5341 ttaatgcgat caatgctgca taggctactt ttaacagctt ctttgtatag tatcttgcag
     5401 cttcataatc tccagctccg atgcatcttg atacaacggt cagcactgca aatcctactc
     5461 cgattcctgg gattaactca aatgatgcta ctacatttcc gatggcattg gctgtgatag
     5521 atgatgtccc aaatcctgca acgatactta ataccatgat tcttcctagc tggaacatac
     5581 tattttcaat tccgtttggt actccgatat gtaaaatctt ctgtattaaa tatcccttaa
     5641 aatgaaaatg gatatgtttg cttaaatgca catcctgatt ttgttttgcg agcagtctta
     5701 ccataacaac tgctgcaaca attcttgata ctaatgttgg gatcgcaact ccttcaactc
     5761 caaatttaaa cccatagatc aaaatcgcat ttcctgcgat gttgataccg ttcatcaaca
     5821 tagacatatt catagaaatc tttgaattcc ccatgatacg gaataatgcg gcgcatccat
     5881 tatatattgc aataaatgga atagatgccg tgacgatcat cagatatatt tccgcgttat
     5941 gcataactcc tggttcaata tttccaaaca ctacatttaa aataaagttt ttacatgcat
     6001 aaattcctgc catgatcact acagataatg aaacaataaa taagatcaac tggtctgctg
     6061 tcttacatgc catgatattt tttctctgtc cgagaaactg ccctgcaacg acggctcctc
     6121 cagttgccaa tgccgaaaac agattgatca ttaataccat aacactgtct accaatgaaa
     6181 ctcctgatac agcttcttct ccaacattcg ctgtcatgat agagtccgca agtcctaccg
     6241 ttacagttag catctgctca atgatcaacg gtatgatcaa tcgtacgaga tctttatttg
     6301 aaaacatcct gttccttgca ctgtctgtaa tttccatatt atttcctttc tttttctgtg
     6361 ttttaaagat tatttctgtg ttttgtcttt tattttacag tttttatctt atcacacttt
     6421 tgattttgtt caaggtctga ttaaaaatat ttacattttc tcatttttat acaaaaaata
     6481 tacaaggtat cttttgatac cttgtataca ttgcatatca tcgtttttta tttcacataa
     6541 tccatgataa tttcaactgc tttctggatt cctgtccttg ctgaattgat catgagatca
     6601 taattaccgg ggtctcccca tttttgtttt gtataataat tatgataaga agatctctcc
     6661 caatcggttt gcttgatcat cttcttcgct tgtcgttctg tcacatgttc tgtctctacg
     6721 atccgtttga tccgttcttc tagaggactg tggataaaga aactgtagca gttttcgtga
     6781 tccttaagca ccacatctgc acatcttccc acgaaaatgc aggattcatt ctctgctgcc
     6841 agttcatgga tcacatgtgc ctggatcata aataatttct ctggtgtcat gatctcttct
     6901 gtgtcaacgc ctggaaatct gtgtccgatc gggacattga taatgtggct gtagactgca
     6961 ttctcctcgt gattctttac aaactcttcc gacatatcac tttcttcggc aactcttgcc
     7021 aaaatctctt tgtcgtagaa aggaattcca agcttcttgg ataactgctg tccgacctct
     7081 cttcctccac tgccgaattc tctggaaatg catatgatcg tatttttctt catattgcaa
     7141 cacccctttt cttgaattac tttatgtatt cctttggtaa ttgtattaac catcctgtga
     7201 ctggttttat tcttatatca aaaaattaca aatttctttt tcatctttat caggatattt
     7261 tcttattaaa ttatatattt tctcaataat ttcttgatct tctaacagat catctgctat
     7321 tttatctacc gtatctcctt tttcaatttt tctctgtact agtgtcacta attttaaaag
     7381 ttcacctttc cttattccta tattctctgc acttcttaat tgactcgctt tatcgcggat
     7441 tgccatttgt tcccttaaat aaagatatct ctcactctca tcttggctca tcttaatcat
     7501 ttcatccctc accttttcca tgtaatgatt tccttcagat tcttctctga tttcttccca
     7561 agtacttgct gaaattaatt tagcccattt ataaagatct tgttttcttg cttttccttt
     7621 tgtcgttgct attttactta attctatcac atgaaattga aatttactac tatacaattc
     7681 acctgttgta tcatctttta ataaaattct atgataaaat ccaggacttt ttaaataatt
     7741 aaaatcaaga attcctacat gaatacatgg aggtaattca taataagatt ctcccttttt
     7801 taatccctct gtaaacatcc gacaattata aaataaactt ctctctgccc aatcttcctg
     7861 atacgtattt tgcatctcga tattaatctt tttgccacca tttaagatta atttgacatc
     7921 tagtattccc tctttttctt cttttacttc accttccaca aaaggatccg taacctccaa
     7981 ctcttcaata tctgtttcct ttaaatgcaa caatgctatt aaaaatcctt ttactatctc
     8041 ttcattctta aatatctttt gaaatgcata attattggtc aaacaaatat ttaattcttc
     8101 cttacttgct gctctctttc ttaattgcgc ttcattgaaa tctgtaatgt ctttattccc
     8161 catattttct ccttatatta caaattatgt aaattgtttc atttatattt taacagattt
     8221 ccatttattg tcaattattt cttacctttt aattttgctt ccttacaatc gcatattcat
     8281 cgtccatctg gaaataaggg caatcccaat cgctatagct cataaatctt gccatctcat
     8341 cttcatctaa attgatatca catacataat attcataatc ttcatcatac acataattac
     8401 tacaatattc gcaccctgcg cttttcttct ttgccatata ttctctcctt atttttgtgt
     8461 ttcttctata tctaatgtta cgccctgtaa aatctctgca aattccagca aagattcaca
     8521 ttttccaaat aatatatcat cgactttact tgtatcctgc acgatatctg ggatcatcac
     8581 tggattcatg cctgctccat gtgcggaacg gattccattg atcgcatctt ctaatgcgat
     8641 cgcttttgat ggattgactt ttaattgtct gcatgcttcg atataaatat ctggctctgg
     8701 ctttccgtgt tcgatcatat ttcctgtgat cacggaatca aagtagtcaa aaatcccttc
     8761 tcgtttcaga ttacttacgg cgtgttcttc actggaagat gttgcaactc cgatctttag
     8821 tcctttttct ctgaggactt tcaaaatctc atgaagacct ttcttggctg gcacaccgtc
     8881 ttcaaccaat tcataatatg catctctgta tccatccaaa aatttatcaa atggaaaatc
     8941 atttccgtaa tgttctaaga aatatttctt acgatttgta aggtttgttc ccaatgtatt
     9001 gacaatatta tgccccagag gtccatatcc cagacgttct cctgcaatat cccatgactt
     9061 ctgtacatag cgttcactgt caaacatcag cccgtccata tcaaaaacaa cagcctgtat
     9121 ctcttttaat ttattaagat ccatcgtctc tattccgcct tccttgttga tcatctttct
     9181 atgataccac ggattccctt tgatttcatc tggtttacaa aaatcgcact gactgttatc
     9241 atggatttta ggattaggag gaactattta tgaaatcatc atcgaattta aaatcactat
     9301 taaaaaatat tgaccgtaaa ggatatcctg catataaaaa tacgcaaggt acttatgatt
     9361 ttggaactta ttttctttcc attgatcatg tacaaggcga tccttttgca tctccatcaa
     9421 aattaagtat tcatgtgaaa ggaagatcgg ctggatttcc tgcttctttt tatgatacaa
     9481 aatataaaca ggttgcactc tgtgatcatt tgacacgatt attttatcaa actttatcta
     9541 agatctcttt tcgtgccaaa ggttctggaa aaagcggttt attttctgtc agtaaatgtg
     9601 gacaacaggt actagaacga actgcatgta cgatcaatcc ttccaacggt gacatctctg
     9661 ttcattttga agctggtttc cctgcaaatg gacgaactgt aaatgcgaga gaattggata
     9721 aaatgttgtt tggactgctg ccagattgtg tttcatcttc tttgttttat aaaaatattg
     9781 atcaaaaaat gttggaatca gtaatttatt tatccgaaga tcaacataca atccgtaaaa
     9841 aactgtcttc tatgggactt gtcgcattta ttgcagatgg ctctgttttg cctagagaaa
     9901 gtggtatctc acagaaacct ttaagaaact gtgtgaaatt ccagtcacca gatacttacc
     9961 gtgtatcttt tgaacttcca catcaaggaa tgatcacggg tatgggaatt ccgaaaggaa
    10021 tcaccctgat cgtcggtggt ggatatcatg gaaaatcgac gttattaaag gcattggaac
    10081 ttggtgttta cgatcatgtc aaaggggatg gtcgtgaatt tgtcattact gatccaactg
    10141 ccatgaagat tcgtgcagaa gatggaagaa gtattacaaa tacagatatt tctatgttca
    10201 ttaataacct gccaaatgga aa
//
LOCUS       DAOMUE010000037        10146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1618_length_10146_cov_2.395005, whole genome shotgun sequence.
ACCESSION   DAOMUE010000037 DAOMUE010000000
VERSION     DAOMUE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1618_length_10146_cov_2.395005"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PLC71_03105"
     CDS             <1..650
                     /locus_tag="PLC71_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01667.1"
                     /translation="LEKVYEPTGDIKNTGCMSDVVVDEDGTIYFANDSKYIMSVGKKV
                     EDTKKSDVITVKPVTKTVKSTRSVKLTWKKKKSAKGYVIYAKAGKGKYKKVTTVGKKT
                     SKTIKVNKDSSYKFKVKPYKLTKKNKKKYFKAYAAKAKFGSKTVKVTFKNVTGYASYK
                     VQMKAGKAAYKTVKTTTKGGTITYTKKKAKVGTTYKFRLKGINKVNGKTVSVTIK"
     gene            850..2967
                     /locus_tag="PLC71_03110"
     CDS             850..2967
                     /locus_tag="PLC71_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRM01668.1"
                     /translation="MKEKINVSEIFGSHVFNDSVMKARLPKAVYKDLKKTIEEGTELN
                     PAIADVVANEMKEWAIEKGATHYTHWFQPLTGVTAEKHDAFINPTDDGKVLLEFSGKE
                     LIKGEPDASSFPSGGLRATFEARGYTAWDCTSPAFIKETDNACILCIPTAFCSYKGEA
                     LDKKTPLLRSMQALDIQTTRLLNVLGNKNVKRVSTSVGPEQEYFLVDEEKYKQRKDLI
                     FTGRTLFGAMPPKGQEMDDHYFGIIKPRIEGFMKDLNIEAWKLGISAKTEHNEVAPAQ
                     HELAPIYNSNNVATDHNQLLMETMRRVARRHGLKCLLHEKPFAGINGSGKHNNWSMVT
                     NEGKNLLDPGKTPHENQQFLLILASIIAAVDKHADLLRMSASTPGNDHRLGANEAPPA
                     IISIFLGDQLEDVVKQLVTRGEATESKHGDKLASGVHSLPAFEKDATDRNRTSPFAFT
                     GNKFEFRMVGSTQSISDPNVVLNTIVADVFADVCDRLEKVEGGVEAVAMEAHEITKEL
                     LTQHQRVIFNGNGYSDEWVAEAERRGLPNLHSMVDAIPTLTTDKAVKMFEKFGVYTKE
                     ELESRVEVLYEQYSNTINIEGLATLDIAKKQIVPAVMKYQRKLANGVAELKTVGVDSS
                     VQEKLLEDITANLKDLFAAINKLEADIPEAQALESEAQARCYHDTIFTDMSAVREPAD
                     KLEMLVAKEDWPMPSYGDLIFEV"
     gene            3140..3910
                     /locus_tag="PLC71_03115"
     CDS             3140..3910
                     /locus_tag="PLC71_03115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRM01669.1"
                     /translation="MKKKFFAYMIIGILLFTFLAQGKGTKTYWFQIASQGEGESGFLT
                     NSNLNQKKVYLTFDDGPSDHTAQILDILKKHKVKATFFVVGKETEHAKKMYQRIVLEG
                     HTLAMHSYSHNYDQIYANVGAFSKDLMKLQKYLYDLTDVKPYIYRFPGGSSNHCAKNI
                     KPYIRYVNKKGLLYFDWNALNEDAVNFEQSPQQLNKKILKDVRRQKTSIVLMHDLHET
                     TNTVKALDPLIKTLKKEGYQILPITKNTKPLHHVSIDK"
     gene            3968..4570
                     /gene="yihA"
                     /locus_tag="PLC71_03120"
     CDS             3968..4570
                     /gene="yihA"
                     /locus_tag="PLC71_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRM01670.1"
                     /translation="MIIKSVNLETVCGITSKLPDNTLPEVAFAGKSNVGKSSLINALM
                     NRKSYARISAQPGKTQTINYYNINEELYYVDLPGYGFAKVTQSVKEKWGHMIENYLQT
                     SKQLKMVFLLIDIRHKPSKNDVMMYDWILHNGYRPVIIATKLDKLKRSQVPKHLKEIR
                     TELQMDKETPIFPFSALSKQGRDEIWDFIESMVLQQEEAE"
     gene            complement(4571..5104)
                     /locus_tag="PLC71_03125"
     CDS             complement(4571..5104)
                     /locus_tag="PLC71_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HRM01671.1"
                     /translation="MEQEQLTLYKLMILYLIEKVDFPLSNSQISEFILDKGYTNYFTV
                     QRAFHELEEENMLRVKQIRNMSHYALSDEGSEAIEMFEYQIPNSIKEDIAQFLKEKEY
                     ELRKEASVTADFYPSKGDEYTVNLKIREKENLLLEINLNVVSRDQAVYICDHWEKKHS
                     DVYSMLIEKLLFDEPNK"
     gene            5227..5415
                     /locus_tag="PLC71_03130"
     CDS             5227..5415
                     /locus_tag="PLC71_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01672.1"
                     /translation="MKRKYQWILCAGLIIFVIAAFCVLVSKDNKEEVKGAFVREGGYQ
                     SECLYQSARKCMSCASRD"
     gene            5360..5983
                     /locus_tag="PLC71_03135"
     CDS             5360..5983
                     /locus_tag="PLC71_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AA"
                     /protein_id="HRM01673.1"
                     /translation="MNVYIKAQESVCLVHPEIKIKDIMSLYCTDKDLEQKIKNQSVYH
                     FIGDHDQRKCFSVLMLVEVIKQVDKTLDVINLGAEDFIVYYKRKQEESKIFHYGKIVF
                     VILVAFFGASFSIMTYNTDVGIEELFYNVYEMIMGQAPDGPNILHISYAAGLTIGIIL
                     FFDHAGKIKLNDDPTPFEVQMRLYGRDVNDTLMINAGRKGEEADVDS"
     gene            5970..6386
                     /locus_tag="PLC71_03140"
     CDS             5970..6386
                     /locus_tag="PLC71_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AB"
                     /protein_id="HRM01674.1"
                     /translation="MWIRSFFLIFYGLCAGGLIAASFLAFLSMLGVIPRLAGLTKSMK
                     NARMYESFVALGGILGTLVFLYRWQIKAGYPLLVLYGLFGGIFVGCMIGALAETIKSL
                     PIFSRRLNLRSGIPYVIYGIAFGKMLGCWLYFYIFS"
     gene            6399..6872
                     /gene="spoVAC"
                     /locus_tag="PLC71_03145"
     CDS             6399..6872
                     /gene="spoVAC"
                     /locus_tag="PLC71_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HRM01675.1"
                     /translation="MIQVKKEPSKEEYQQIVKNLTPKHNSLLNCTKAFVTGGTFCVLG
                     EVMKQLMMKYLNLSKKDSLLYVTIELILLSVLLTGFNFYGKITKFGGAGGLVPITGFA
                     NSVASAAIEYQKEGQVFGIGVKIFTIAGPVILYGIFSSFVTGLIYLFTKQWGWFL"
     gene            6869..7882
                     /locus_tag="PLC71_03150"
     CDS             6869..7882
                     /locus_tag="PLC71_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HRM01676.1"
                     /translation="MTSICGMQSLKFENAPYLKGWASVAGKKEGEGPLGNLIDQIIED
                     PYFGQESWELAEGRFMKQAAMLAISKADLHKKDIRYAFAGDLLEQNTATFSGMKELGI
                     PLFGLFGACSTVGEAMSLAAMSVAGGFAKHSLAIASSHIGSAEKQFRFPLEYGNQRPL
                     SATWTVTGSGGFIVSKEIGPVKIQGITTGKIVDYGMEDPMNMGACMAPAAAEVIYQHF
                     VDFGSKPEDYDAVYTGDLGEVGKKILLHLLRENGYDLSKVHGDCGIEIYENETQDTHS
                     GGSGCACSAVVLASMIIPKLIDGTWKKVLFVPTGALMSKTSFNEGENVAGIAHAIFLE
                     GVR"
     gene            7885..8241
                     /gene="spoVAE"
                     /locus_tag="PLC71_03155"
     CDS             7885..8241
                     /gene="spoVAE"
                     /locus_tag="PLC71_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HRM01677.1"
                     /translation="MEYIRAFLVGGIICILVQILMDHTTLQPGRIMVLLVIAGVILGA
                     LGIYKRIEAFGGCGATVPLSGFGFSLWKGMKEAVDNHGFLGLFEGGFTAAAVGTSAAL
                     IFSYLASLIFQPKIKK"
     gene            complement(8326..>10146)
                     /locus_tag="PLC71_03160"
     CDS             complement(8326..>10146)
                     /locus_tag="PLC71_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM01678.1"
                     /translation="NPTRLNPVTHPENNASRRSLVLQNMLEQHYITKKEYREALSDDV
                     YTRIQKNAAAAPAKKTTNSYFEDALILQVVKDLKKQLGYDETKAYNAIYSGGLKIYST
                     QDQQIQKIADSVTNDASNYPKATKIALSYSLSAKTVSGKDVVYSDHNLLDYMRKNNLG
                     NQLIFTDEASAKTVVTKFKQYILSKGETITNESFQTTIQPQISMTIMNQSNGTVEALV
                     GGRGNKTSDLSLNRATGTTRQPGSTFKILSAFLPALDKNHMTLATVYEDAPYNYIDTN
                     RPVRNYYKGYKGYSTIREAITNSMNVVSAKTIADVTPKTSFEYLMNLGFSTLVSNETT
                     SNGNVYTDITQSLSLGGLTYGVTNIELTSAYASIANGGTYQKPVLYTKVVDHNGNILL
                     SNTQKGKRVMKESTAFLLTDAMKDVITKGTGKSAKLSSSMAVAGKSGTTSNSYDYWFS
                     GYTPYHTASVWMGYDKNTNFASDNTHKKIWAKVMNEIIKTKQEQTTDFKKPADIVKAK
                     VCKESGKLAIKGVCDHDPRGSRVITEYFEKGTVPTQTCDIHVAVEVCKTSDKLATKNC
                     PANKKQRKIYIVRKKGSHGKTADSPYMLPKKYQSVFCKKH"
BASE COUNT         3442 a         1615 c         2147 g         2942 t
ORIGIN      
        1 cattggaaaa agtttatgaa ccaactgggg acataaagaa tacaggatgt atgagtgatg
       61 tagtggttga tgaagatggt acaatttatt ttgcaaatga ttctaaatat attatgtcag
      121 taggcaaaaa agttgaagat acaaaaaaat cagatgtaat cacagtaaaa ccagtcacaa
      181 aaacagtaaa aagtacaaga agcgtaaaac tgacatggaa aaagaaaaaa tccgccaaag
      241 gctatgtcat ttacgccaaa gcaggaaaag gtaaatacaa aaaagtaaca accgtaggaa
      301 agaaaacatc aaagacgatc aaagtcaaca aagactcatc ctacaaattt aaagtaaaac
      361 catacaaact aacaaagaaa aacaaaaaga aatattttaa agcctatgca gccaaagcca
      421 aattcggaag caagacagtt aaagtaacat ttaaaaatgt cacaggatat gcaagctaca
      481 aagtgcagat gaaagcagga aaagcagcat ataagacagt aaaaacaaca accaaaggcg
      541 gaacgatcac atatacaaag aaaaaagcca aagtaggaac aacttacaaa ttccgcttaa
      601 aaggaatcaa taaggtaaat ggaaaaactg tttccgtaac gatcaaatag aagtactgga
      661 taaaatacaa aaaagggctt gattttcaaa aagaatcatt gtatcctata accagtgaag
      721 ttactgacaa gggcgtcaaa caattagttt catattgttt gtcgcccctt tttttctact
      781 atcagttaga taatagaaga aaaatatata gattatgctt caaatgtaag agaagaaagg
      841 aagaactcta tgaaggaaaa aatcaatgta tcagaaatct tcggatccca cgtatttaac
      901 gacagtgtta tgaaagcacg tcttccaaag gccgtttaca aagatctgaa aaagacaatc
      961 gaagaaggaa cagaattaaa tccagcaatt gcagacgtag ttgcgaatga gatgaaagaa
     1021 tgggctattg aaaagggtgc tacacattat actcactggt tccagccatt aacaggtgtg
     1081 acagcagaga aacatgacgc tttcattaac ccaacagacg atggtaaagt tttacttgag
     1141 ttctcaggaa aagaactgat caaaggtgaa ccagatgcat cttcattccc atctggtgga
     1201 ttaagagcaa catttgaagc aagaggatat acagcatggg attgtacatc accagcattc
     1261 atcaaagaaa cagataatgc ttgtatctta tgtattccaa cagcattctg ttcatacaaa
     1321 ggtgaagcat tagacaagaa gacaccactt cttcgttcta tgcaggcatt agatatccag
     1381 acaacaagat tattaaatgt attaggaaat aagaatgtga aacgtgttag cacatccgtt
     1441 ggaccagaac aggaatattt cttagttgat gaagaaaaat ataaacagag aaaagacttg
     1501 atcttcactg gacgtacatt attcggagca atgcctccaa aaggacagga aatggatgac
     1561 cattactttg gaatcatcaa accaagaatc gaaggattca tgaaagattt aaatatcgaa
     1621 gcatggaaac ttggaatcag tgcgaagaca gaacataacg aagtagctcc agcacagcac
     1681 gaattagcac caatctataa ctcaaacaac gtggcaacag accacaacca gttattaatg
     1741 gaaacaatga gacgtgtagc aagacgtcat ggattaaaat gcttacttca tgagaaacca
     1801 tttgcaggaa tcaatggatc aggaaaacat aacaactggt ctatggtaac aaatgaagga
     1861 aagaacctgt tagatccagg aaaaactcct catgaaaacc agcagttctt actgatctta
     1921 gcatctatta ttgcagcagt ggataaacat gcagatttac ttcgtatgtc agcttccaca
     1981 ccaggaaatg accacagatt aggagctaac gaggcacctc cagcaatcat ctctatcttc
     2041 cttggagatc agttagaaga tgttgtaaaa cagcttgtaa caagaggtga ggcgacagaa
     2101 tccaaacatg gagacaaatt agcatctggt gttcattcat taccagcatt tgagaaagat
     2161 gcaacagacc gtaacagaac atcaccattc gcatttacag gaaacaaatt cgaattccgt
     2221 atggtaggtt ctacacagtc tatctctgat ccaaacgttg tattaaatac aatcgtagca
     2281 gatgtctttg cagatgtatg tgacagatta gagaaagtag aaggcggagt tgaagcagta
     2341 gctatggaag ctcacgagat cacaaaagaa ttattaacac agcatcagag agttatcttt
     2401 aatggtaacg gatattctga cgaatgggta gctgaagctg agagaagagg acttccaaac
     2461 ttacacagca tggtagatgc aatcccaaca ttaacaacag ataaagcagt taagatgttt
     2521 gagaaatttg gagtatacac aaaagaagaa ttagagtctc gtgttgaagt attatatgaa
     2581 cagtattcta atacaattaa cattgaagga cttgcaacat tagatatcgc taagaaacag
     2641 attgttccag cagttatgaa ataccagaga aaacttgcaa atggcgttgc tgaattaaag
     2701 acagttggtg ttgacagctc tgtacaggaa aaattgttag aagatatcac agcaaactta
     2761 aaagatctgt ttgctgcaat caacaagtta gaagctgata ttccagaagc tcaggcatta
     2821 gaaagtgaag ctcaggctcg ctgctatcat gatacgattt tcacagatat gagtgccgta
     2881 cgtgaaccag cagacaaact ggaaatgtta gtagcaaaag aagactggcc aatgccaagt
     2941 tacggagatc tgattttcga agtataataa ttacatagtt tgtaaaactt cttaatacac
     3001 aaagagaaag cagcagagaa atctgttgct ttctttttgt gtatcaacca ttgtttgaaa
     3061 aatattccag aatatatttt ttgaaatttc atattctaaa gaaaaagaat atcttacaaa
     3121 attagaaaag aggaagaata tgaaaaagaa attttttgca tatatgatca tagggattct
     3181 tctgttcaca tttctggcac aagggaaggg aacaaagaca tactggtttc agattgcaag
     3241 tcagggtgaa ggggaaagtg gctttttaac caacagtaat ttgaatcaaa agaaagttta
     3301 tttaaccttt gatgatggac catccgatca tacggcacag attcttgata ttctaaagaa
     3361 acataaggtg aaggcgacat tctttgtcgt aggaaaagaa acggaacatg ccaagaaaat
     3421 gtatcaaaga atcgtgttag aaggtcatac attggcaatg cattcctaca gtcataatta
     3481 tgatcagatt tatgcgaatg taggtgcatt ttcgaaagac cttatgaaac tacagaaata
     3541 tttatatgat ctaacagatg taaagccata tatttaccgg tttccgggtg gcagcagtaa
     3601 tcattgcgca aaaaatataa aaccgtatat ccggtatgtg aacaaaaagg gattacttta
     3661 ttttgactgg aatgcattga atgaagatgc ggttaatttt gaacaaagtc ctcagcagtt
     3721 aaacaagaag attttaaagg atgtcaggag acagaaaaca agtattgtat taatgcatga
     3781 ccttcatgag acaacaaata cagtgaaagc attagatccg ctgatcaaaa cattgaaaaa
     3841 agaagggtat cagatccttc cgatcactaa aaatactaag ccgctccatc atgtatccat
     3901 tgacaaataa tgggctttca cgtagaatat gttcggtaaa ggatttattt tacgtgagga
     3961 gaatgacatg atcataaaaa gtgtaaattt agaaaccgtc tgtgggatca caagtaaatt
     4021 accagacaat acattgccag aagtggcatt tgctggaaaa tcaaatgtag gaaaatcatc
     4081 actgatcaat gcactgatga accgcaaatc ttacgcgaga atttcagcac agcctggaaa
     4141 gacacagacg atcaattatt acaatattaa cgaagaactt tattatgtag accttccagg
     4201 atatggattt gcgaaagtaa cacaatccgt caaggaaaaa tggggacaca tgatcgaaaa
     4261 ttacttacag acatcaaaac agctaaaaat ggtcttttta ctgatcgata tcagacataa
     4321 gccatcaaag aacgatgtta tgatgtatga ctggattctt cacaatggat atcgtccagt
     4381 gatcattgca acaaagcttg ataaactaaa gagaagccag gtgccaaaac atctaaaaga
     4441 gatccgcaca gagcttcaga tggataagga aacaccaatt ttcccattct cagcattatc
     4501 gaaacaggga agagatgaga tttgggattt cattgagtca atggtgcttc aacaagaaga
     4561 agccgaataa ttatttattc ggctcgtcaa ataacaattt ttcaattagc atagaataga
     4621 catcggagtg ttttttctcc caatggtcac atatgtaaac tgcctggtct cttgagacaa
     4681 catttaagtt gatctcaagg agcaggtttt ctttttctct gatcttaaga tttacggtat
     4741 attcgtctcc tttggatgga taaaaatcag cagtcacact ggcttcctta cgaagctcat
     4801 attctttttc ttttaaaaac tgagcgatat cttctttgat agagtttgga atctggtatt
     4861 caaacatctc aattgcttca gatccctcgt cactgagcgc gtagtggctc atattacgaa
     4921 tctgcttgac tctgagcata ttttcttcct ctaactcatg aaaagctctc tgaacagtaa
     4981 aataattggt atatccctta tcaaggataa attcagaaat ctgtgaattg ctcaaaggga
     5041 aatctacctt ctcaattaaa tataaaatca taagcttata aagtgttaat tgttcttgtt
     5101 ccatggctga tctcctttga tttatcgata aaagtcacta tgaaaaatta taacaccaca
     5161 tgatttttct tgcaacttat gaatgaaaag ttaaaaatga cagataataa cgctgaggtg
     5221 ataagaatga aaagaaagta ccagtggatc ctatgtgcag gactgatcat ttttgtgatc
     5281 gcagcgtttt gtgtcttagt atccaaagat aacaaggaag aagtgaaagg tgcatttgta
     5341 agagaaggag gatatcaaag tgaatgttta tatcaaagcg caagaaagtg tatgtcttgt
     5401 gcatccagag attaaaataa aggatatcat gtccctgtac tgcaccgaca aggatttgga
     5461 acagaaaata aagaatcaaa gtgtgtatca ttttatagga gatcatgatc agagaaaatg
     5521 tttttctgtt ttgatgttag ttgaagtgat aaaacaggtg gataagacat tagatgtgat
     5581 caatttaggt gccgaagatt ttattgtata ttacaagaga aaacaagaag aatcaaagat
     5641 ttttcattat ggaaagatag tctttgtaat tttagtcgca ttttttggtg caagtttttc
     5701 aattatgaca tataacacgg atgttgggat cgaagaattg ttttataacg tttatgaaat
     5761 gatcatggga caggcaccag atggtccaaa tattcttcat atttcttatg cagcaggatt
     5821 gacgatcggg atcattctat tttttgatca tgcaggtaag atcaagttaa atgatgatcc
     5881 aacaccattt gaagtacaga tgaggcttta cgggagagat gtgaatgata cattgatgat
     5941 caatgcaggg cggaaagggg aagaagcaga tgtggattcg tagtttcttt ctgatctttt
     6001 acggattgtg tgcaggcggc ttgatcgcag ccagttttct ggcatttttg agtatgttag
     6061 gtgtgatccc gagattagca ggattgacga agagtatgaa aaacgccaga atgtatgaaa
     6121 gttttgtggc acttggaggg attcttggga cattggtctt tctttatcga tggcagatca
     6181 aagctggata cccgcttctt gtactgtatg gattgtttgg aggaatcttt gtgggatgta
     6241 tgatcggagc tttggcagaa acgatcaaaa gtcttccgat cttttcaaga agattaaatt
     6301 tgagatcagg gattccatat gtaatctatg ggatcgcatt tggaaaaatg ctaggatgtt
     6361 ggctttattt ttatatattt tcataaaatg aagaaagggt gatacaagtg aaaaaagaac
     6421 caagcaaaga agaatatcaa cagatcgtga aaaatcttac accaaaacat aatagtctgt
     6481 taaattgcac aaaagcgttt gtaacgggtg ggacattttg tgttcttggt gaagttatga
     6541 agcagttaat gatgaaatat ttgaatctct ccaagaaaga ttcattatta tatgtgacaa
     6601 ttgaattgat cttactaagt gtcttattaa caggatttaa tttctatgga aaaattacaa
     6661 aatttggcgg tgcaggcggg cttgtgccga ttacgggatt cgcaaattca gtggcttcag
     6721 ctgcaatcga atatcagaaa gaaggacagg tttttggtat tggcgttaag atttttacga
     6781 tcgcaggacc agtgatctta tatggaatct tctcatcatt tgttactgga ttgatttatt
     6841 tattcacaaa gcaatggggg tggtttctat gacatcgatt tgtggtatgc agagtctaaa
     6901 gtttgagaat gcgccgtact taaaaggctg ggcatcagtt gcagggaaga aagaagggga
     6961 aggtcctctg ggaaatctga tcgatcagat cattgaagat ccatactttg gacaagaaag
     7021 ctgggaattg gcagaaggcc gttttatgaa acaggcagca atgcttgcga tcagtaaagc
     7081 tgatcttcat aagaaggaca tccgttacgc gtttgcgggt gatcttttgg aacagaatac
     7141 agcaacgttt tctggaatga aagaactagg gataccgttg tttggacttt ttggagcatg
     7201 ttcaacggta ggagaagcga tgtctctggc agcgatgagc gttgcaggag ggtttgcaaa
     7261 gcattcactt gcaattgctt ccagtcatat cggaagtgct gaaaaacagt tccgttttcc
     7321 gttagaatat ggaaatcaga gaccattatc tgcgacatgg actgtgacag gatctggagg
     7381 ttttattgtg tcaaaagaaa ttggtcctgt aaagattcaa gggatcacaa caggaaagat
     7441 cgttgattat gggatggaag atccgatgaa tatgggggct tgcatggcgc cagcagcggc
     7501 agaagtaatc tatcagcatt ttgtggattt tgggtcgaag cctgaagatt atgatgcagt
     7561 atatacagga gatcttggag aagttggcaa gaagattttg ttgcatttgc ttcgtgaaaa
     7621 tggatatgat ctttcgaaag ttcatgggga ttgtggaatt gaaatctatg agaatgagac
     7681 gcaggatact cattctgggg gctccggctg tgcctgtagt gcagtggttc ttgcatcgat
     7741 gatcataccg aagctcattg atggaacgtg gaagaaagta ttgtttgtgc caacaggagc
     7801 attaatgtca aagacaagtt ttaatgaagg tgaaaatgtt gcagggatcg cacatgcgat
     7861 ctttctggaa ggagtaagat aataatggaa tatatcaggg cgtttttagt aggaggcatt
     7921 atttgtatcc tggtgcaaat attgatggat catacgacat tacagccggg aaggatcatg
     7981 gtactcttag ttattgcagg tgttattctt ggggcactgg ggatttataa aagaatcgaa
     8041 gcgtttggag gatgtggtgc gaccgtccca ttgtctggtt ttggattcag tttatggaaa
     8101 ggaatgaaag aggcagttga caatcatggg tttctagggt tattcgaagg aggattcacg
     8161 gcagcagcag tcggaacatc agcagcattg atcttttctt atctggcatc tttgatcttt
     8221 cagccgaaga tcaagaaata aaggcaagag tatgaaataa agagaaaaaa gggattctga
     8281 tttgagagaa tcccttttta atataaaaat atagaagatc aacggttaat gtttcttgca
     8341 gaaaacagat tgatattttt ttggaagcat ataaggagag tctgctgttt ttccatgact
     8401 gccctttttg cggacaatgt aaatttttct ttgttttttg tttgcaggac aattctttgt
     8461 ggcgagttta tcgcttgttt tacatacttc aactgcaacg tggatatcac aggtctgggt
     8521 aggaacggtt cctttttcaa aatattctgt gatcacacgg ctgcctcttg gatcgtgatc
     8581 acaaacccct ttaatagcaa gttttccaga ctctttgcag acttttgctt ttacaatgtc
     8641 agctggtttt ttaaaatctg tagtttgttc ttgttttgtt tttatgattt cattcataac
     8701 ctttgcccat atttttttat gagtgttatc agaagcaaaa tttgtattct tgtcatagcc
     8761 catccagaca gaagctgtat gataaggagt ataaccgctg aaccagtaat cgtaactgtt
     8821 tgaagtagtt cctgattttc ctgcgacagc catagatgaa gaaagttttg ctgattttcc
     8881 agttcctttt gtaataacgt ctttcatagc gtcggttaaa aggaaagcag tactttcttt
     8941 catgacacgc ttacctttct gagtattaga aagaagaata ttaccattat gatctacgac
     9001 ttttgtgtat aaaacaggtt tctggtaggt tccgccattt gcaatggaag catatgcaga
     9061 agtcaattct atatttgtaa caccataagt caggccacca agagataagg actgggtaat
     9121 atcggtatat acatttccat ttgaagttgt ttcattagac accaaagtgg aaaaaccaag
     9181 attcatcaaa tattcaaacg aagtcttagg tgtaacgtca gcaatggttt tggccgaaac
     9241 aacgttcatg gagtttgtga tcgcttcgcg gatcgttgaa taacctttat aacctttgta
     9301 atagttgcgt actggtctgt tagtgtcaat atagttataa ggagcatcct catatacggt
     9361 tgcaagtgtc atatgattct tatcaagtgc aggtaaaaat gcagaaagaa tcttaaaagt
     9421 agaacctggt tgtcttgtag taccagtagc acgattcaag gaaagatcgg aagtcttatt
     9481 tccacgtcca ccaactaagg cttctacagt tccgttagat tgattcatga tcgtcatgga
     9541 tatctgaggc tggatcgttg tttggaaaga ttcatttgta atggtttcac cttttgataa
     9601 aatatattgt ttaaactttg taacaactgt ctttgcactt gcttcatctg taaaaatcaa
     9661 ctgatttcca aggttatttt ttctcatata atcaagcaga ttgtgatcag aataaacaac
     9721 gtcttttcct gaaacagttt ttgcagataa agaataagaa agcgcgattt ttgtggcttt
     9781 tggatagttt gaagcatcgt ttgtgacgct atctgcaatc ttctggatct gttgatcctg
     9841 agtagagtat attttcaatc cgccactgta aatagcatta taagcttttg tttcgtcata
     9901 ccccagttgc ttttttaaat cttttacaac ttgaaggatt aatgcgtcct caaaataaga
     9961 atttgttgtt ttttttgcag gtgcagcagc agcatttttt tgaattcttg tgtatacatc
    10021 atcactaagt gcttctcgat attctttttt tgtgatataa tgttgctcta acatattttg
    10081 taaaacaagt gatcttctgg aagcgttgtt ttccggatga gttacaggat ttaaacgtgt
    10141 cggatt
//
LOCUS       DAOMUE010000038        10073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1633_length_10073_cov_2.346377, whole genome shotgun sequence.
ACCESSION   DAOMUE010000038 DAOMUE010000000
VERSION     DAOMUE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10073
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1633_length_10073_cov_2.346377"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLC71_03165"
     CDS             <1..341
                     /locus_tag="PLC71_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRM01679.1"
                     /translation="RSIMGTEIPYVKYDAHENQLTYAMDGTFESIDIAREAFRQVKDL
                     TIKLSMVENAAYRLATSIKKTQKRANALKNITIPTYTGLVYKISNELEEKEREEFTRL
                     KVIKRMKQKG"
     gene            complement(775..>981)
                     /locus_tag="PLC71_03170"
     CDS             complement(775..>981)
                     /locus_tag="PLC71_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01680.1"
                     /translation="RGVLMNRIKLAVIADDFTGGADAASFLKRKHANVVLLTGIPTSL
                     ILWDHKANILVLAIIFSTVVEAAC"
     assembly_gap    982..991
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<992..1582)
                     /locus_tag="PLC71_03175"
     CDS             complement(<992..1582)
                     /locus_tag="PLC71_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01681.1"
                     /translation="MQIIKTSVIENIKHNFEELFPAEKKTAQYILDHLEEVTLLNISQ
                     LAKKAHASEASIVRMAKHLGYNGFFQMRLLLSNDLLCAAKLICEARMCHIIGAGNTIP
                     IASDLRFRLQRNGQSCMYSSLPEQYFNNIALGDSSGQVFGGHIVKGYTPTLTTVDLII
                     FEIKNIEMLRAHLHHFLTALNLAYADPARPQQAGSAT"
     assembly_gap    1720..1729
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1832..2728
                     /gene="gnd"
                     /locus_tag="PLC71_03180"
     CDS             1832..2728
                     /gene="gnd"
                     /locus_tag="PLC71_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HRM01682.1"
                     /translation="MKLKMVGLGKMGFNLALNMKDHEVDVEGFDVNEEARKKAEESGI
                     KSYETLKDLVSADQKDVIWVMLPAGKITNSVLDELSGLCKKGDIVIDGGNSDHRDSLK
                     TAKKMEEKGIYFFDIGTSGGVYGARHGASFMCGGDPEVFKNDLQEMLESIATTNGCLY
                     TGKTGSGHYLKMIHNAMLYGYMQTLGEGFELLEKSEFDYDLEHVADSLSKSSVIRGWL
                     LELAANAFRKDPDLEKIKGVVGASKTTGWTIESACELGVPIPIISTSLMMRLRSKQDD
                     SFSAKVIASLRDEVGGHKAQSK"
     assembly_gap    2739..2748
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2749..3058
                     /locus_tag="PLC71_03185"
     CDS             <2749..3058
                     /locus_tag="PLC71_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01683.1"
                     /translation="WFYKIERDEKNKYINITWNTTMTYIKSILGVVGVGACLFNLVWL
                     AAICLTILAVALTYYLYRYGNLVRILHSHEQKKGLEYTGSQYSFKDPLIVTIPQVRNI
                     "
     gene            3055..4266
                     /locus_tag="PLC71_03190"
     CDS             3055..4266
                     /locus_tag="PLC71_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HRM01684.1"
                     /translation="MNRQENLIQILLKAEKPLTTQELAEQLNVSSRTIRSDLEKIESE
                     ILVHSMKLEKKPRVGIWIEGTQDEKDALFLDVKGEHDLVESYSKEYRRGCILVQILLS
                     KNKIYPYKLQNNLYVSKSTIEKDLQEISKWLEKYDLSLMKKPSIGLYVSGDEENIRNA
                     VAALAGELSEKNQSIESLMETYLDIDVKEIEDIIHNWNDNYNMHLNEVNINNLAFHAS
                     VMLMRIGKNKALLIENSKALDSETFSYKEEFDILINELSTYGNCEIPKDEADYLLMHL
                     LGMYLNESSFLENDFLNDLRKIAENIAEDFILKSDKIMSLDLESNDQFKRSLMLHLLP
                     TVYRLKYGLNLYNPLLNEIKTNYASYYYLALIINSSFKKYIGVNASEGEIAYVALHLS
                     VAVQRLSVLLE"
     assembly_gap    4478..4487
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4488..4974
                     /locus_tag="PLC71_03195"
     CDS             <4488..4974
                     /locus_tag="PLC71_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM01685.1"
                     /translation="IVDVCSFLNRRKNPSINKTNFSMQTVKVFHEQIDKIQILQEMSN
                     YLRMCGAVTPRFFEGVLKRENMGSTEVGDGIILTHGFHEDVKRTQIAFCKLDHPIVWK
                     TQEVDFIVMLAVAKTDAKNVMQMNWLYKMLSNMEIVNKIRECKKDREIYETLIEASKK
                     L"
     gene            4990..5469
                     /locus_tag="PLC71_03200"
     CDS             4990..5469
                     /locus_tag="PLC71_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM01686.1"
                     /translation="MNLLDILDLDLIDLDMDATDKDGVLKQLSSMLYKKGNIKDLDKF
                     LEAVYERESIGETGLGGIAIPHGLTDQVINASVAIGKVKQPVEWESLDDQPVSLIFLL
                     AAPTGDLQKTHLQNLSQLASVVAHKAHVDALMKCETKEEFFELFETYFNEFTKRKEA"
     gene            5472..5792
                     /locus_tag="PLC71_03205"
     CDS             5472..5792
                     /locus_tag="PLC71_03205"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HRM01687.1"
                     /translation="MNIVGITSCTCGIAHTYMAREKLLETGEKFGWSVKIETQGSGGV
                     EFALTDEDINAADCVLIASDVAVSGTERFKGKPMVKVPVATAIKSPEHLLRKIEEKLG
                     ALKK"
     gene            5815..6876
                     /locus_tag="PLC71_03210"
     CDS             5815..6876
                     /locus_tag="PLC71_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HRM01688.1"
                     /translation="MLKDVFKNLKQHCMSGISYMIPVIVIGGFCTALARLAGNVDTAG
                     TIGYAFLQAGNAAFALMMSVLCAGIAYSICGKPGIAPGVVAGYLSTQVKASFLGALIC
                     GFLIGIMILWMQEHFPDSKALKSMYPIVIYPVISGIIATLLIMFVFGPPLAALTQAAV
                     DFFMNMNTGSKFLLGFILGCMTGFDMGGPVNKICFSVVSAFAASGIWGPAAGKNAAAM
                     APPMGMAISALVLTPKKYTEQEREDAKVAIAMSLCQVTEGALPFAFNDPKRVIPAVTI
                     GSGVAHGLILTWGVTVPVLHGGIFSVPLASNPMLWIAAWLIGAMVTAVIVSVTKPVRP
                     VETVHEDEELKDDFDIEIG"
     gene            6967..7998
                     /locus_tag="PLC71_03215"
     CDS             6967..7998
                     /locus_tag="PLC71_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008818428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01689.1"
                     /translation="MKIEKKYLDEMKRCYCASNISFDGENHVLLASEDPEVACNMYYG
                     KDFEKKQNVWTIPGGCMSIVPIPGKEKEFLAVQEFYLKVTPSLAKIVWGKYEDGEWKF
                     KDVVSIPYVHRFGIFHANGINYLIAATVATSKKEKNDWSVPGRIYVAELPEDPSKGVE
                     LEILVDGLYRNHGFWQTKKGGKDVGYFGSDQGILRVSAPEKRGGQWKTELIMEGHIGE
                     IATIDIDNDGEDEIMTIEEFHGDTIQIYKKIDGKYTKVWKYDNEIDFAHALVGTKLAG
                     QNAFVCGVRRKDCELFAVTYEDGEYKVTMIEKGVGPANLCVVNEKDRDIIVAANHTAN
                     EAAVYFVTE"
     gene            8031..8732
                     /locus_tag="PLC71_03220"
     CDS             8031..8732
                     /locus_tag="PLC71_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HRM01690.1"
                     /translation="MLEELKQKVLEANLLLPQHGLVTFTWGNVSEIDREKEIVAIKPS
                     GVEYSKMTAEDIVLVDLEGNILEGELRPSSDVDTHLEFYRNWPGIGGVVHTHSTWATG
                     FAQAGKDIIALGTTQADYFDGAVPCTRFMTDEEIKGSYELETGKVIVEEFQKREIDPE
                     RVPGALVHSHGPFTWGSDGFNAVHNAVVLEECAKMNAIAMLVKNPEIGSMQQTLLEKH
                     FNRKHGPGAYYGQSK"
     gene            8716..9558
                     /locus_tag="PLC71_03225"
     CDS             8716..9558
                     /locus_tag="PLC71_03225"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HRM01691.1"
                     /translation="MVNQNKEYYLGLYEKSMPNTLSWEEKLKVVKEAGFDYLEMSIDE
                     TEEKLARLDQSVDEIEKAIEKTGVPIKSICLSGHRKYPLGSHDPKIRERGMEIMEKAI
                     CLAARLGVRVIQLAGYDVYYEESDFQTRDYFKMNLKEAAIMAAKQGVLLGFETMETPF
                     MDTVEKAMAYVKDVDQAYLGVYPDIGNLKNASLLYNVDVNEDIMTGKGHIFATHLKET
                     VPGKYREIPFGTGHTEFVRNIKTLKRLGVRMFVGEFWYVGNDDWKQVIIDANDFLRDK
                     LEQA"
     gene            9693..10073
                     /locus_tag="PLC71_03230"
                     /pseudo
     CDS             9693..10073
                     /locus_tag="PLC71_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285556.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3596 a         1440 c         2168 g         2829 t
ORIGIN      
        1 caagaagtat catgggaaca gagattccat atgtgaaata tgatgcacat gagaatcagt
       61 tgacgtatgc gatggatggg acatttgagt caattgatat tgcaagagaa gcgtttcggc
      121 aggtgaagga tctgacgatc aagctttcta tggtggaaaa tgcagcttat cggttggcaa
      181 cgagtattaa gaagacacag aagagggcaa atgctcttaa aaatattacg attccaactt
      241 atactggatt ggtttataag atttcgaatg agctggaaga gaaggaaaga gaggaattta
      301 cacggcttaa agtgattaag cggatgaagc agaaaggatg aatttaatcc aaacaaaagg
      361 acagaagatt gccctcaatc atcaatcttc tgtcctttat gacatttcca tgaaatcaca
      421 tctccatagc tttcaaaaag aaatcctcac tatcaaaatt tccagatttc aatataagct
      481 gcaaatgcct attttcttag ctgttgtata aaatgcaggg gattttttct gttttaaaat
      541 ctttcaacac agcatcacta tatattaaca atggactttt ctgcgcatca ataagtgatg
      601 taatgtaagt tagatcatca ctggtttcat tgatagataa acattcataa ttttcgctaa
      661 aatgttgtat ttgcagcctg gtagcttttg aacagcttcc acataaaatg atcgtgcggt
      721 tatcaaaaga tgtcttaaaa ttttcctgcg gggaagaaaa atcaaaaaga aattctaaca
      781 agccgcttcc accactgtag agaaaattat cgccaaaaca aggatatttg ctttgtgatc
      841 ccataagatt agggatgtgg gaattcctgt taataagaca acatttgcgt gcttccgttt
      901 cagaaaactt gcagcatcgg ctccgcctgt gaaatcatct gcaatcacag caagtttaat
      961 acgattcata agtacacccc tnnnnnnnnn nagtcgctga tcccgcctgc tgagggcggg
     1021 cgggatcggc gtatgccaaa ttcagtgctg tcaggaagtg atggagatgc gcacgcagca
     1081 tttcaatatt tttgatctca aaaataataa gatcaaccgt agtcagtgtt ggagtatatc
     1141 cttttacaat gtggccacca aagacctgac cagaagaatc accaagtgca atgttattaa
     1201 aatactgttc tggcaatgaa gaatacatac aggattgccc gttcctctga aggcggaatc
     1261 taagatcaga agcaatcggg attgtatttc cagcccctat aatatgacac attctggctt
     1321 cacagatcaa ttttgcagca cagagcagat catttgaaag aagaagccgc atttggaaga
     1381 aaccattata ccctagatgt tttgccattc tgacaatgga agcttcactt gcatgagctt
     1441 ttttagcaag ctgtgagata ttaagaaggg ttacttcttc aagatgatca aggatatatt
     1501 gtgcagtttt tttctctgca ggaaagagtt cttcaaagtt atgtttaata ttttcgatga
     1561 ctgatgtttt tataatttgc atggacagct cctttctgat actaagaaag ttaacacgaa
     1621 ttataaagtg tgtcaatttg atatctttca aaaagtggaa atgcgaaaag tggaaatgtg
     1681 gaaattcaaa tgcggaaatg gaggaaaatg tggaaatatn nnnnnnnnna cctttggttt
     1741 ggaatagact aaaaaatata gaaatgtcac aaaagaaatc atataataaa cccatcgaaa
     1801 gacaaaagtg acaaaagaaa ggagccaagt catgaaatta aaaatggtag gtcttggaaa
     1861 aatgggattt aatttagccc tgaatatgaa agaccacgag gttgatgtag aaggattcga
     1921 tgtaaatgaa gaagccagaa agaaggctga agaatctgga attaaatcat atgaaactct
     1981 aaaagatctg gtatctgcgg atcagaaaga tgtgatctgg gttatgttac cagctggaaa
     2041 gatcacaaat tcagtattag atgaattaag tggtttatgt aaaaaaggag acatcgtgat
     2101 cgatggtgga aattcagatc atcgagacag cttaaagaca gcaaagaaga tggaagagaa
     2161 aggaatttat ttctttgata ttggaacaag tggaggggtt tacggagcaa gacacggagc
     2221 aagttttatg tgtggaggag atccggaagt atttaaaaat gatcttcagg aaatgttaga
     2281 aagcattgca acaacaaacg gatgtcttta cacaggaaaa acaggaagtg gtcattatct
     2341 taaaatgatt cacaatgcaa tgttatatgg atacatgcag acattaggag aaggatttga
     2401 acttcttgaa aaaagtgaat ttgattacga tctggaacat gttgcagatt cacttagtaa
     2461 atcaagtgtt attagaggat ggctgttaga acttgcagca aatgcattta gaaaagatcc
     2521 agatctagaa aagatcaaag gagttgttgg agcaagtaaa acaacaggat ggacaatcga
     2581 gtctgcttgt gaacttggag ttccaatccc aattatttct acttcactga tgatgcgttt
     2641 aagatcaaaa caggatgatt cattttcagc gaaagtgatc gcatctttaa gagatgaggt
     2701 tggaggacac aaagctcagt caaaataaag gagaaattnn nnnnnnnngt ggttttacaa
     2761 gattgaaaga gatgaaaaaa ataaatatat caatatcaca tggaatacga caatgacata
     2821 catcaaaagt attctaggag ttgtcggtgt aggagcatgt ttgtttaatc ttgtatggct
     2881 tgcagcaata tgcttaacga ttttagctgt ggcactgaca tattatttat atcgatatgg
     2941 aaatctagtt cgaatacttc atagccatga acaaaaaaag ggattagaat atacaggtag
     3001 ccaatattca tttaaagatc cattgatcgt aacaattccg caggtgagaa atatatgaac
     3061 agacaagaaa atttaattca gattctttta aaagcagaaa aaccacttac aacacaagaa
     3121 ttagcagagc agttaaatgt cagcagccgg acaatccgat ctgatcttga aaaaatagaa
     3181 tcagaaatcc tggttcattc catgaaactt gaaaagaaac caagagttgg tatttggatt
     3241 gaaggtacac aagatgaaaa agatgcattg tttcttgatg taaaaggaga acatgactta
     3301 gtagagtctt attcgaaaga ataccgaaga ggctgcattt tagtacagat attgctgtca
     3361 aaaaataaaa tatatccata taaattacag aataatcttt atgtaagcaa gtcaacaatt
     3421 gaaaaagatc ttcaggagat ctcaaaatgg ctggagaaat atgatcttag tttaatgaag
     3481 aaaccaagta taggtcttta tgtcagtgga gatgaagaaa atattcgaaa tgcagttgct
     3541 gcactagcag gtgaactgag tgaaaaaaat cagtccattg aaagcttgat ggaaacatat
     3601 ttggatatag atgttaagga aatcgaagac attatacata actggaatga caattataat
     3661 atgcacttaa atgaagtgaa tataaacaat ctggcttttc atgcatcggt tatgttaatg
     3721 agaataggta aaaataaagc attattaata gaaaattcaa aagccttgga tagtgagaca
     3781 ttttcttata aagaagagtt cgacatccta ataaatgaac tttcaacata tggaaattgt
     3841 gagattccaa aagatgaagc agattatctt cttatgcatc ttttaggtat gtatttgaat
     3901 gaaagctcat ttttagaaaa tgatttttta aatgatctga gaaagattgc tgaaaatata
     3961 gcagaagatt ttattttgaa atccgataag atcatgtcat tagatttaga atcaaatgac
     4021 cagtttaaaa gatcactgat gcttcatttg cttccaacag tatatcgttt gaaatatgga
     4081 ttaaatctat ataacccatt attgaatgag attaagacta attatgccag ctattattat
     4141 ctggctctga tcatcaattc aagtttcaaa aaatatatcg gagtaaatgc aagtgaagga
     4201 gaaattgctt atgtggcatt gcatttgtca gtagctgtac agcggttgtc tgttctcttg
     4261 gagtaggagt atcaagatta ctttctgtaa agcttcagga aaacttccca gatgtaacat
     4321 ttattcattg ttccatgaat gatgaggaac agttagaaaa atgtaaatat atcatttcaa
     4381 cagtggaact taacacagac aaaccttatg tacaagtaaa tccgctgctg atggaagtag
     4441 atgtacagaa aatacgaaca ctattaagaa aaaatccnnn nnnnnnnaat tgttgatgtt
     4501 tgttcatttt taaatcgaag aaaaaatcca agtattaata aaacaaattt ctccatgcag
     4561 acagtaaaag tctttcatga gcaaattgat aagattcaga tcttgcaaga gatgtcaaat
     4621 tatcttcgca tgtgcggcgc agtgacacca agattttttg aaggtgtttt aaaaagagaa
     4681 aatatgggat ctacggaagt aggagatggt attattctga cacatggatt ccatgaggat
     4741 gtaaagcgaa cacagattgc attttgcaag ctggatcatc cgattgtctg gaagacacag
     4801 gaagttgatt ttatcgtaat gttagcagtt gcaaaaacag atgcaaaaaa tgtaatgcaa
     4861 atgaactggt tgtataaaat gttaagcaat atggagatag tcaacaagat aagagaatgc
     4921 aagaaagaca gagaaatata tgaaacatta atagaagcaa gtaaaaaact ttaaaggagg
     4981 gtacatatta tgaatttatt agacattctg gatcttgatt taatagatct ggatatggac
     5041 gcaacagata aagatggagt tttaaaacag ttaagttcca tgctttataa aaaaggaaat
     5101 attaaagatc tggataaatt cctggaagcg gtttatgaaa gagaaagcat tggagaaaca
     5161 ggtcttggag ggattgcgat tcctcatggt ttaacagatc aggtaatcaa tgcaagtgtt
     5221 gcaatcggaa aagtaaaaca gccagtagaa tgggaaagcc ttgatgatca gccagtaagt
     5281 ctgattttct tattagcagc accaactgga gatcttcaga agacacatct tcagaatcta
     5341 tcacagcttg ctagcgttgt agcacataaa gcacatgtcg atgcgttaat gaaatgtgaa
     5401 acaaaagaag aattttttga actatttgaa acttatttta atgaatttac aaagagaaag
     5461 gaagcttaat tatgaatatt gttggaatta catcttgcac ttgtggaatc gcacatactt
     5521 atatggctag agaaaaactg ttagaaactg gagaaaaatt tggatggagt gtaaaaatcg
     5581 aaacacaggg aagcggtggt gttgaatttg cgttaacaga tgaagatatc aatgctgcag
     5641 attgtgtatt gatcgcatct gatgttgctg tatctggaac agaaagattc aaaggaaaac
     5701 caatggttaa agtacctgtt gcaacagcaa tcaaatcacc agaacatctt cttcgtaaga
     5761 ttgaagaaaa attaggtgca ttgaagaaat aaaagtactg aaaggagaga agatatgtta
     5821 aaagacgtat ttaaaaactt aaagcagcat tgtatgagtg gtatctctta tatgatccct
     5881 gttatcgtta tcggtggatt ttgtacagca cttgctcgtc tggcaggaaa tgttgataca
     5941 gcaggaacta ttggatatgc attcttacag gcaggaaatg cagcatttgc attaatgatg
     6001 tccgtacttt gtgcaggaat tgcttactct atctgtggta aaccaggaat tgcaccaggt
     6061 gtagtagcag gatatttgtc aacacaggta aaagccagtt tccttggagc attaatttgt
     6121 ggattcttaa ttggtattat gatcttatgg atgcaggaac atttcccaga ttcaaaagct
     6181 ttgaaatcaa tgtatccgat cgttatttat ccggtaatat ccggaattat agcgacgctg
     6241 ttgatcatgt ttgtctttgg accgcctctt gcagcattaa cacaggcagc tgttgacttc
     6301 tttatgaata tgaacacggg atcaaaattc ttattaggat ttatcttagg atgtatgacc
     6361 ggatttgata tgggaggtcc tgtgaataaa atttgtttct ctgttgtcag tgcattcgca
     6421 gcctctggaa tctggggacc tgcagccgga aaaaatgcgg cggctatggc acctccaatg
     6481 ggtatggcaa tttcagcact ggtattaact cctaaaaaat atacagaaca agaaagagaa
     6541 gatgcaaaag ttgcgattgc aatgtcattg tgtcaggtaa cagagggtgc attaccattt
     6601 gcctttaatg atccgaaacg agtcattccg gcggtaacaa ttggttcagg tgttgctcat
     6661 ggattaatct taacttgggg agtaacagtt cctgtactgc atggtggaat cttctcggtt
     6721 ccactggcaa gtaatccaat gttatggatt gcagcatggt tgatcggagc tatggttaca
     6781 gcggtaattg tttctgtaac aaaaccagtg cgtccagttg aaacagtaca tgaagatgaa
     6841 gaattgaaag atgattttga tattgaaatt ggataatcag acaattcgca gtaaaacgta
     6901 ttttatagaa gaatagagaa gctgcattat gcagcttttc ttatacccaa aaaaggagaa
     6961 aaaaccatga aaattgagaa aaaatattta gatgagatga aaagatgcta ctgtgcaagc
     7021 aatatttcct ttgatggaga aaatcatgta ctacttgcaa gtgaggatcc agaagttgcc
     7081 tgcaacatgt attatggaaa agatttcgaa aagaaacaaa atgtctggac aataccaggt
     7141 ggatgtatgt caatcgttcc aattccagga aaagagaagg aatttcttgc agtacaggaa
     7201 ttttatttaa aagtaacacc atctttagct aagatcgtat ggggaaaata tgaggatgga
     7261 gaatggaaat ttaaagatgt agtatctatt ccatatgtgc atcgttttgg tatttttcat
     7321 gcgaacggaa ttaattatct gatcgcagca acggttgcaa catccaaaaa agaaaagaat
     7381 gactggagtg taccgggacg tatttacgtt gcagaattac cagaagatcc atcaaaaggt
     7441 gtagaacttg aaattttagt agatggatta tatagaaatc atggattttg gcagacaaaa
     7501 aaaggtggaa aagatgtcgg atattttgga agtgatcagg gaatccttcg tgtcagtgca
     7561 ccagagaaac gaggaggcca gtggaagaca gaactgatca tggaagggca tatcggagaa
     7621 atagcaacga ttgatattga taatgacggt gaagatgaga tcatgacaat cgaagaattt
     7681 catggagata cgattcagat ttataaaaaa attgatggaa aatatacaaa agtctggaag
     7741 tacgataatg aaatcgattt tgcacatgcc cttgttggaa caaaattagc tggacagaat
     7801 gcatttgtat gtggcgtaag aagaaaagat tgtgaattat ttgctgttac ttatgaagat
     7861 ggagaatata aagtcacaat gatagaaaaa ggagtaggtc ctgcaaatct gtgtgtagtt
     7921 aacgaaaaag acagagatat cattgtagca gcaaatcaca cagcaaatga agcagctgta
     7981 tactttgtaa cagaataaaa cctgaataca tttaataagc gagggatttt atgttagaag
     8041 aattaaaaca gaaagtatta gaggcaaatt tattattacc acagcatgga ttagttacat
     8101 ttacatgggg aaatgtatca gagattgacc gggaaaaaga aattgttgca attaaaccaa
     8161 gtggagtaga atacagcaag atgacagcgg aagatattgt tcttgtagat cttgaaggaa
     8221 atattcttga gggagaatta agaccaagtt ctgatgtaga tacacatttg gaattttatc
     8281 gaaactggcc aggaattggc ggtgttgtac atacacattc cacatgggca actggttttg
     8341 cacaggcagg aaaagatatt attgcgttag ggacgacgca ggcagactat tttgatggag
     8401 cagttccatg taccagattc atgacagatg aagaaataaa gggaagctat gaattagaga
     8461 caggaaaagt gattgtagag gaattccaaa aaagagaaat tgatccagag agagtaccag
     8521 gagctttagt acatagtcat ggaccattta catggggaag tgatggattt aatgcagttc
     8581 ataatgcggt tgttttagag gaatgtgcaa aaatgaacgc aattgcgatg ctggtcaaaa
     8641 atccagaaat tgggtcaatg cagcagacat tattagagaa acattttaac cgtaaacatg
     8701 gtccgggggc gtattatggt caatcaaaat aaagaatatt atctgggact ttatgagaaa
     8761 agtatgccta acacactttc atgggaagaa aaactgaaag tggtaaaaga agcaggtttt
     8821 gattatctgg aaatgagcat tgatgagaca gaagaaaagc ttgcaagact tgaccagtca
     8881 gtggatgaaa tagaaaaggc aatcgaaaaa acaggagttc ctataaaatc tatttgctta
     8941 agtggtcatc gaaaatatcc attaggctca catgatccaa agattaggga acgaggaatg
     9001 gaaatcatgg agaaagccat ttgtctggca gcaagattag gagtacgagt catacagctt
     9061 gccgggtatg atgtatatta tgaagaaagc gattttcaaa caagagatta ttttaagatg
     9121 aatctgaaag aggctgcgat catggcagca aagcaggggg tattgctagg atttgaaaca
     9181 atggaaacac cttttatgga tacagtagaa aaggctatgg catatgtaaa agatgtggat
     9241 caggcatatc ttggagtata tccagacatc ggtaatctga aaaatgcttc tcttctatat
     9301 aatgtagatg tgaatgaaga cataatgaca ggaaaaggtc atatttttgc aacacattta
     9361 aaagagacag ttccaggaaa atatagagaa atcccatttg gaacaggaca tactgagttt
     9421 gtaagaaata taaagacttt aaaaagactg ggagtcagaa tgtttgtagg agaattctgg
     9481 tatgtcggaa atgatgactg gaaacaggta attatagatg caaatgattt cttaagagat
     9541 aagctggaac aggcataata gaataaattt attgaaaaat ggaattgtaa taagacagtt
     9601 ccattttttt gtatgtatta tttaaataac accactttac aacttttttc ataaaaaaat
     9661 atcgtacaga actatctgat gtataatgaa aaatacatcc ggcttttcta tgtgttgatg
     9721 ataccatgat atccaagttt ggcaaaaaat ttgaaaatgt ttcaaaactg ttcgatcatg
     9781 ttgcacacaa tggttccaac tatctcaatg gacactgttt tgtaagtatc atgatatgcg
     9841 tgcctgtttt ggatcatgat aagatatctt acctgtgtta ttgatgaata tccaaacctt
     9901 gatctcattg gcaatgcaag gattgattct gtcaagtatg atctcgctcc cgaacggact
     9961 gggcgcagag gacgtcctgc caagcatgga aaacgtctat ttttcagcac aatcttttca
    10021 gaagatctga aggtcttttg tacggaggag gaacactcat cttctgacca gac
//
LOCUS       DAOMUE010000039         9671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1734_length_9671_cov_2.701643, whole genome shotgun sequence.
ACCESSION   DAOMUE010000039 DAOMUE010000000
VERSION     DAOMUE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9671
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1734_length_9671_cov_2.701643"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /gene="pepT"
                     /locus_tag="PLC71_03235"
     CDS             complement(<1..1193)
                     /gene="pepT"
                     /locus_tag="PLC71_03235"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRM01692.1"
                     /translation="MKAYERLLNYVKILTPSNENSQTVPSSDCQFDLARVLENEMKEL
                     GLSDVYLDDKCYLYGKLPATKGYENCPAIGFIAHMDTVSDFCDHPIRPILTKNYNGEA
                     LPLGDSSLVLDPKVFPHLSSLKGRTLITSDGTTILGADDKAGIAEILTMVERLISKEI
                     PHGPISIAFTPDEEIGSGAEYFDIKRFDADFAYTLDGDTEGEIQFENFNACKAEFEIT
                     GFNVHPGSSKDTMINASLVAMEINSCLPSMETPRNTEDYEGFYHLIDMNGDVSHATLH
                     YIVRDHDQGLFEAKKQTLRLIAKNMNEKWGNGCVTLTITDQYQNMAEIIKDCPHLIKN
                     AKKACENAGVAPLVLPIRGGTDGCQLSFRGLPCPNLGTGGHAYHGPYEHISVEGMDMC
                     VDIVV"
     gene            1351..1542
                     /locus_tag="PLC71_03240"
     CDS             1351..1542
                     /locus_tag="PLC71_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01693.1"
                     /translation="MFITFLQMGLANEVLLIPLLYLLNAVDGVIYGQALTDYIAVILS
                     TIIWKQYQNSITKEKDSVK"
     gene            1865..3415
                     /locus_tag="PLC71_03245"
     CDS             1865..3415
                     /locus_tag="PLC71_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HRM01694.1"
                     /translation="MFIHFLLAMLPIIWLIIALSGLKMAGHVACPIALIITVIEALFL
                     WKQKIIDVLTGGLEGFAMAIWPICLVIVAAVFTYNLVVHTKNMELIKKMLTSVSRDKR
                     VLVLIIAWGFGGFMEGMAGFGTAVAIPAGILCGLGFEPIFAATVCLVANTTPVAFGSI
                     GIPTVTAANVTGFSPHMTASYVVLQLAIMVILVPFLVVFITGKHEGAKGIGDYKEILF
                     ITLMSGLSFLVPQYLTAKFVGAELPAVIGSVCSMAVTIILAKVMLKGKASKFDVEIED
                     NDDTTMTVKDAFVAWSPFILVLLFLLLTSTLVPAIHDPLSAIKSNVPIYSGEGASPYT
                     FTWVSTPGVLILIAAFIGGLIQGCPVGEIFVVLGKTVIQMFKTIITIMAVLATAKIMG
                     YSGMTQSIADFIVRVTGSFYPLVAPLIGSIGTFVTGSSTSSSVLFSKLQASTGAELNI
                     NQIWLVAANTVGSTAGKIISPQSIAVATAATATVGKESEILTKVIKYFVLFVIIYGLV
                     CYFGVKLI"
     gene            3471..4898
                     /locus_tag="PLC71_03250"
     CDS             3471..4898
                     /locus_tag="PLC71_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRM01695.1"
                     /translation="MSEYQYNKVTPEMIEKFKEIAPKRVLVGDEINEDFTHDEMAIYG
                     KARPEVLVEATSTEEVAAVVKLCNENKVPVTPSGARTGLVGGAVSIGGGVMISLTKMN
                     KILGYDKENFVVKIQSGVLLNDLAQDAEKQGLLYPPDPGEKFATVGGNVATNAGGMRA
                     VKYGCTRDYVRAMTVVLPTGEIVKLGATVSKTSSGYSLLNLMIGSEGTLGIITELTLK
                     VIPAPKSVISLIIPYENLEDCIATVPQFFMHHLAPQALEFMEKEVVMDTEKFLGKQVY
                     PKELEGTEIGAYLLATFDGNSEEQLEDIIEQASEVVLEAGAIDVLVADTPALKKDAWA
                     VRGALLEAIEADTVLLDECDVVVPTNKIAEFLTYTKSLEAEADFRVKSFGHAGDGNLH
                     IYTCSNDMEEGEFKKQVAVFMDKVYAKATEFGGMISGEHGIGHGKMDYLAESLGPVQM
                     RIMEGVKEVFDPNMILNPGKICYKL"
     gene            4987..6123
                     /locus_tag="PLC71_03255"
     CDS             4987..6123
                     /locus_tag="PLC71_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM01696.1"
                     /translation="MAYLISEEAKDLLQDVHEFCENEVKEQCKAYDVSGEWPKEIYDK
                     AIEMQLHSLEVPEEYGGPGLSRVDVAALIEEMAIADAGFATTISASGLGTKPVLIAGN
                     EEQKKHVCQKIIDGGFGAFCLTEPGAGSDASAGTTTAVKDGDEWVLNGRKCFITNGGI
                     ASFYCITAMTDKTKGVKGISMFLVDAGTPGLSTGNEENKMGIRTSNTCDVVLEDCRIP
                     AANLVGEEGRGFSIAMKTLDQARAWMGCVATGIAQRGINEGIAYAKERVQFGKPVIKN
                     QAMQFKVADMEIKTETARQMVAHALTKMDMGLPHSKEAAIAKCYASDIAMEVASEAIQ
                     MFGGYGYSREYPVEKLLRDAKIFQIFEGSNEIQRIVVANNTIGR"
     gene            6145..6948
                     /locus_tag="PLC71_03260"
     CDS             6145..6948
                     /locus_tag="PLC71_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRM01697.1"
                     /translation="MNILVCIKQVPDDSVEIHLNAEGTPDLANVTPVVNAFDTYALEM
                     AARLKESSEGEVTVVCVGEDSAKNSLKNCLAVGADHAFLVSDDAFKGSDTTGIANILK
                     NVIAKLEADNGQKFDLVFCGKEATDAALGQGGLMLAEELGTSVITNITEIALDGDKVT
                     AKQETEEGYRTVEASAPCVVTVTKPEYDPRYPTIKNKMAARKKPIGDIKEADLADLEK
                     EKVGESNAKVQIVKLYEPAKKEAGIKIQEETPEDSAIKAVAMMADAKVF"
     gene            6967..7944
                     /locus_tag="PLC71_03265"
     CDS             6967..7944
                     /locus_tag="PLC71_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRM01698.1"
                     /translation="MAECKNIMVYVETAEGNPVKVGLEMLSPAKKLADKVTAVVIGGG
                     VADAAKEVAKAGADQVICVDSEDYKEFNLDAYAQVLVELVKEENPEAVFVGGTQDGKD
                     IAPVVAAKCNTGCASDVLDIKAEDGEVIYTCPLYGGTVLEDVKIKTTPQIATLRSGAF
                     QKVEDPTEGEVVAKEVKVADDVIKAKVTESVKEIAETINLEEAEVIVSGGRGMGSKEN
                     FALVDDLAKLLGGVVGATRPAIEDGWVSKIHQVGQSGKIVAPKLYIACGISGATQHVS
                     GIMNSGYIVAINKDEDAPIFDVANVGIVGDVMKVLPVMIEEIKKIKDAQ"
     gene            complement(8073..8279)
                     /locus_tag="PLC71_03270"
     CDS             complement(8073..8279)
                     /locus_tag="PLC71_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HRM01699.1"
                     /translation="MGNKCLDCTALTLTIIGAINWGLIGFFRFDLVAFLFGDMSWLSR
                     VVYALVGLCGLYMLAFYRHCGRDI"
     gene            8462..8962
                     /locus_tag="PLC71_03275"
     CDS             8462..8962
                     /locus_tag="PLC71_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD domain-containing protein"
                     /protein_id="HRM01700.1"
                     /translation="MKVIFLDVDGVLNSQQLFEKCEDDQLISVDEDNIKNLKTIVDAT
                     GAKIVLSSSWRYGWAEHSDAVQDWCQILVDILAKYDLKIIDKTEYLSSGRREDEIKDW
                     LDKCEEKIEGFVILDDGAYEWHRHGFDKHLVKTDFCTGGLREEDADKAIKILNKKRLF
                     SFFKKY"
     gene            8977..>9051
                     /locus_tag="PLC71_03280"
     CDS             8977..>9051
                     /locus_tag="PLC71_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRM01701.1"
                     /translation="MDINKVTTAMIDYYQGQPKRIQHFL"
     assembly_gap    9052..9061
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9062..9468
                     /locus_tag="PLC71_03285"
     CDS             <9062..9468
                     /locus_tag="PLC71_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM01702.1"
                     /translation="VHAYAKLIGEQEGLDKEILDILEVAALTHDIGIKISEEKYNSSA
                     GKYQEVEGPAVAQQMLEDLQYDKAKTDRVCYLIGHHHTYDQIDGIDYQILVEADFLVN
                     LAEEQSSRETIESVKGKIFKTKTGIWLINKIF"
     gene            9541..9613
                     /locus_tag="PLC71_03290"
     tRNA            9541..9613
                     /locus_tag="PLC71_03290"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9574..9576,aa:Phe,seq:gaa)
BASE COUNT         3423 a         1621 c         2088 g         2529 t
ORIGIN      
        1 tccacaacaa tatcaacaca catatccatt ccttcaacgc taatatgttc ataaggtcca
       61 tgataagcat gtccaccagt tccaagattc ggacaaggaa gtcctcgaaa acttaactga
      121 catccatctg tacctccgcg aatcggcaga accaatggag ctacaccagc attttcacaa
      181 gccttctttg catttttgat caaatgagga caatccttaa taatctcggc catattttga
      241 tactgatccg taatggttaa agtaacgcaa ccattacccc atttttcgtt catatttttc
      301 gcgatcaaac gtaatgtctg tttcttcgcc tcaaacaacc cctgatcatg atctctcaca
      361 atgtaatgta atgttgcatg actaacatca ccattcatgt cgatcaaatg atagaatccc
      421 tcataatctt ccgtgtttct aggagtctcc atagaaggta gacaagagtt gatctccata
      481 gctacaagtg aggcattgat catcgtatcc tttgaagaac caggatgtac attaaaacca
      541 gtaatctcaa actcagcttt gcaggcatta aaattttcaa actggatctc accctcggta
      601 tcgccatcca aagtataagc aaaatcagca tcaaaccgct tgatatcaaa atactcagca
      661 ccacttccaa tttcttcatc tggagtaaat gcaatgctga tcggaccatg aggaatctct
      721 ttactgatca gacgttctac catcgtaaga atctcagcaa tcccagcctt atcatctgca
      781 ccaaggatcg ttgtaccatc cgatgtgatc aaagtacgac ccttgagaga agaaagatga
      841 gggaaaactt tcggatcaag aaccagacta ctatctccaa gaggaagagc ttctccatta
      901 taattcttcg tgaggatcgg acggatcggg tgatcacaga aatcagaaac agtatccata
      961 tgagcaataa atccgatcgc aggacagttt tcatagccct ttgtggcagg taatttgcca
     1021 tataggtaac atttgtcatc taagtagaca tcactgagac caagttcctt catttcattt
     1081 tctaagactc gggcaagatc aaactgacaa tcagaagaag ggacagtttg gctgttttca
     1141 ttgcttggag ttaaaatttt gacgtaattt aataaacgtt cgtaagcttt catgattatt
     1201 tattcacctc gtgataattt cttttgcgtt ttataaagta tatagataat ataacacata
     1261 tctagtgtat caaccatcgt attaaactag actctcgtag tctatgggaa tgcaccagtt
     1321 gcagcaatcg gaattgtatt caaagcaaat atgtttatta catttttaca aatgggacta
     1381 gccaatgaag ttttgctgat cccgttattg tatcttttaa atgcggttga cggagtcata
     1441 tatgggcagg cattgacaga ctatattgca gtgatattgt ccacgatcat atggaagcaa
     1501 tatcaaaata gtataacgaa agaaaaagac tccgtaaaat agttcgaaat acgaaatttg
     1561 tggaaattga tgaaaaatta acaaataaat aaaaaacagt atttatacaa atggcttaaa
     1621 atggttggat tgaaaatagt atgtttgtaa aaatgctgct ttttttgtgc aatttctaca
     1681 aaaaagaaaa gaaaatagaa aaagaataaa tcgttttgca aaaaaaatcc cttgactttt
     1741 tgtcggattg gtcctaccat ataagtatag taactggtag gaccaatctt actatgtcgc
     1801 gtatacgctg aaaaatagga tatcggaacc cgctcatatt tgaaaagaaa ggagagaact
     1861 taagatgttc attcactttt tattagcgat gcttcctatc atctggctga tcattgcatt
     1921 aagcggatta aagatggcag gacatgtagc ctgtccgatc gctttgatca ttacagtcat
     1981 tgaagcactt tttttatgga agcagaaaat tattgatgtc ctcacaggag ggttagaagg
     2041 atttgcaatg gcaatctggc caatctgtct ggttatcgtg gcagcagtgt ttacttacaa
     2101 cttggtcgta catacgaaaa acatggaact tattaaaaaa atgcttactt ctgtatcaag
     2161 agataaacga gttcttgtgc tgatcatcgc atggggattt ggaggattta tggaaggtat
     2221 ggcaggattc ggaacagctg tagcgattcc agcaggaatc ctttgcggat taggatttga
     2281 accaatcttt gcagcaactg tttgcctggt agccaatacg acaccagtag catttggatc
     2341 catcggaatt ccaacggtta cagcagcaaa tgtaacagga tttagtccac atatgacagc
     2401 atcttatgtt gtattacaac ttgcgatcat ggtaattctc gttcctttcc ttgttgtttt
     2461 catcacagga aaacatgaag gagctaaagg aatcggtgat tataaagaaa ttttatttat
     2521 tacattgatg tcaggtcttt cattcttagt accacaatat ctgacagcaa aatttgtagg
     2581 agctgaactt ccagcagtta tcggatctgt atgcagtatg gcagtaacga tcatccttgc
     2641 aaaagtaatg ctgaaaggta aggcaagtaa atttgatgta gaaatcgaag ataatgatga
     2701 tactacgatg acagtaaaag atgcatttgt tgcatggagc ccattcattc ttgtattact
     2761 gttcttactt ctgacttcaa cattagtacc agcgattcat gatccattat cagcgatcaa
     2821 atcaaatgta ccaatttata gtggagaggg agcaagccca tatacattta catgggtatc
     2881 aacaccagga gtgctgatct tgatcgcagc atttattgga ggattgattc agggatgtcc
     2941 agttggtgag atctttgttg ttttaggcaa gacagtgatt cagatgttta agacgatcat
     3001 tacgatcatg gcagtgcttg cgacagctaa gatcatggga tacagcggaa tgacacagtc
     3061 tatcgcagat tttatcgtac gtgtaacagg aagcttttat ccattagttg caccactgat
     3121 tggatctatc ggaacattcg taacaggaag ttctacatca agcagcgtac tattctccaa
     3181 gttacaggca agtacaggag cagaattaaa tatcaaccag atctggctgg tagctgcaaa
     3241 tacagttggt agtaccgcag gaaagattat ttctccacag agtatcgcag tagcgacagc
     3301 ggcaacagca acggtaggaa aagaaagtga aatcttaaca aaggttatca aatatttcgt
     3361 attattcgtt atcatctatg gattagtttg ctactttgga gtcaaattga tctgaaaatg
     3421 ataaagataa aagatattaa aaagctataa acttttaagg aggaagataa atgagtgagt
     3481 atcagtataa caaagtgaca ccagagatga tcgagaaatt caaagagatc gctccaaaac
     3541 gtgtcttagt gggtgatgaa atcaatgagg atttcaccca tgatgagatg gcaatctacg
     3601 gaaaagctag accagaagtt ttagtagaag caacatcaac agaagaagtt gcagctgtcg
     3661 ttaaactttg taatgaaaac aaggttcctg taacaccaag tggtgcaaga acaggattag
     3721 taggaggagc cgtaagtatc ggtggcggtg ttatgatttc tcttacaaag atgaacaaga
     3781 tccttggata tgacaaagaa aactttgtag tgaagattca atcaggagtc cttttaaatg
     3841 atcttgcaca ggatgcagaa aaacaaggat tattatatcc accagatcca ggtgagaaat
     3901 tcgcaacagt cggtggaaac gtagcaacca acgctggtgg tatgagagct gttaagtacg
     3961 gatgtacaag agattatgta cgtgccatga cagttgtatt accaacaggt gagattgtaa
     4021 aacttggagc tactgtatca aagacaagtt caggatacag cttattaaac ttaatgatcg
     4081 gttctgaagg tactttgggt attatcacag aacttacatt aaaagtaatt ccagcaccaa
     4141 aatcagtgat cagtctgatc attccttatg aaaacttaga ggattgtatt gcaacagtac
     4201 cacagttctt tatgcatcat ctggcaccac aggctctgga atttatggaa aaagaagttg
     4261 ttatggatac tgagaagttc ttaggaaaac aggtatatcc aaaagaatta gaaggaacag
     4321 agattggagc atatttatta gcaacatttg atggaaattc tgaagaacaa ctggaagata
     4381 tcattgaaca ggcatctgaa gtagtattag aagcaggagc aattgatgta ttagttgctg
     4441 atacaccagc cttaaagaaa gatgcatggg ctgtccgcgg agcattactt gaggcaattg
     4501 aagcagatac tgtactgtta gatgaatgtg atgttgtagt tccaacaaat aagattgcag
     4561 aattcctgac atatacaaaa tcattagaag cagaagctga tttcagagtt aagagctttg
     4621 gacatgctgg agacggtaac ttacatatct atacatgcag taatgacatg gaagaaggag
     4681 agtttaagaa acaggttgca gtctttatgg ataaagtata tgcaaaagca acagaattcg
     4741 gtggaatgat ttctggagaa catggaatcg gacatggaaa gatggactac ctagcagagt
     4801 ctctcggacc agttcagatg aggatcatgg aaggtgtcaa agaagtcttt gatccaaata
     4861 tgatcttaaa tccaggtaag atctgctaca aattataatt ttaataggaa acgattaatc
     4921 aaacataaac aagtaacata aaacatatac aaagcttttt taaggttatt ttaggaggat
     4981 atcaagatgg catatttaat ttcagaggaa gcaaaagact tattacagga tgtacacgag
     5041 ttctgtgaaa acgaagtaaa agaacagtgt aaagcatacg acgtcagtgg agaatggcca
     5101 aaagaaattt atgacaaagc cattgaaatg cagttacatt cattagaagt accagaagaa
     5161 tacggtggac caggattatc aagagtagac gttgcggcac tgatcgaaga aatggctatc
     5221 gcagatgcag gatttgcaac aacaatttct gccagtggat taggaacaaa accagtactg
     5281 atcgcaggaa atgaagaaca gaaaaaacat gtatgccaga agatcattga tggtggattt
     5341 ggagctttct gtttaacaga accaggagca ggatctgatg ctagtgcagg aacaacaaca
     5401 gctgtcaaag acggagatga gtgggtatta aatggaagaa aatgcttcat tacaaatgga
     5461 ggaatcgctt ctttctactg tatcacagct atgacagata aaacaaaagg tgttaaagga
     5521 atctctatgt tcttagttga tgctggaaca ccaggattaa gcacaggtaa tgaagaaaat
     5581 aaaatgggta tccgtacatc aaacacatgt gatgttgtat tagaagactg tcgtattcca
     5641 gctgcaaact tagttggaga agaaggaaga ggattctcaa tcgcaatgaa gacattagac
     5701 caggcaagag cttggatggg atgtgttgca acaggtatcg cacagcgtgg tatcaatgaa
     5761 ggtattgcat atgccaaaga aagagttcag tttggtaaac cagtcatcaa aaatcaggca
     5821 atgcagttta aagtagctga tatggaaatc aaaacagaaa cagctcgtca gatggttgca
     5881 catgctttaa caaagatgga tatgggatta ccacattcca aagaagcagc gatcgcaaaa
     5941 tgctatgcat ctgatatcgc aatggaagta gcttctgaag caatccagat gttcggtgga
     6001 tatggataca gcagagaata tccagttgag aaattattaa gagatgctaa gatcttccag
     6061 attttcgaag gaagtaacga aattcagaga atcgttgtag caaataacac aatcggaaga
     6121 tagaacataa gaaggagtaa gactatgaac attttagtat gtatcaaaca ggttccggat
     6181 gattctgtag aaatccattt aaatgcagaa ggaacaccgg atttagcaaa cgtaacacct
     6241 gttgtaaatg catttgacac ctatgctctc gaaatggcag cacgattaaa agaatcctct
     6301 gaaggagaag taacagttgt atgtgtcgga gaagatagtg caaagaactc tctgaaaaac
     6361 tgtcttgcag ttggtgcaga tcatgcattt ttagtaagtg atgatgcatt caaaggatct
     6421 gacacaacag gaatcgcaaa tatcctaaaa aatgtgatcg caaaattaga agcggacaat
     6481 ggtcagaaat ttgatcttgt attctgtgga aaagaagcaa cagatgccgc acttggacag
     6541 ggtggcttaa tgttagctga agaacttgga acaagtgtga tcacaaatat tacagaaatc
     6601 gctttagatg gcgataaagt gacagcaaaa caggaaacag aagaaggata ccgtacagta
     6661 gaagcttctg caccatgtgt tgtaacagtc acaaaaccag aatatgaccc acgttaccca
     6721 acgatcaaga ataaaatggc agcaagaaag aaaccaatcg gcgatattaa agaagctgat
     6781 cttgctgatc ttgaaaaaga aaaagtcgga gaatctaatg caaaggttca gatcgttaaa
     6841 ttatatgaac cagcgaagaa agaagccgga atcaagattc aggaagaaac accagaagat
     6901 tcagcaataa aagcagtcgc tatgatggca gatgccaaag tattctagga agggagaaca
     6961 aaggacatgg cagaatgtaa gaatatcatg gtatatgtag aaactgcaga aggaaatcca
     7021 gtcaaagttg gattggaaat gttatctcca gccaagaaac ttgccgataa agttacggca
     7081 gttgttattg gaggtggagt agcagatgca gcaaaagaag tagcaaaggc cggagccgat
     7141 caggtcatct gtgtcgacag cgaagattac aaagagttta atctcgatgc atatgctcag
     7201 gttttagtag aattagtaaa agaagaaaat ccagaagctg tatttgtagg tggaacacag
     7261 gatggaaaag atatcgcacc agttgttgca gcaaaatgta acacaggatg tgcatccgat
     7321 gtattagata tcaaagcaga agatggagaa gttatctata catgcccact ttatggtgga
     7381 actgtattgg aagatgtaaa gatcaagaca acaccacaga tagcaacatt acgttccgga
     7441 gcattccaga aagtggaaga cccaactgag ggtgaagttg tagccaaaga agtaaaagta
     7501 gctgatgatg tgatcaaagc aaaggtcaca gaaagtgtaa aagaaatcgc agaaacgatc
     7561 aacctggaag aagcagaagt tatcgtatct ggtggacgtg gaatgggaag caaagaaaac
     7621 ttcgcattag ttgacgatct tgcaaaactc ttaggaggag ttgtaggagc aacaagacca
     7681 gccattgaag acggatgggt atcaaagatt catcaggttg gacagtctgg aaagatcgtt
     7741 gcaccaaaat tatatattgc atgcggaatc tctggagcta cacagcacgt atcaggaatc
     7801 atgaattctg gatacattgt agcaatcaac aaagatgaag atgcaccaat ctttgacgta
     7861 gccaatgtag gaatcgttgg agacgttatg aaagtgcttc ctgttatgat cgaagagatc
     7921 aagaagatca aggatgcaca gtaaataaat atctattttt aaaatagctt gtttctatac
     7981 ttaatgtata ttcaccattt ataaaaggtc agattatggc gtatgccata gcctggcctt
     8041 tctttccgtg tataattaat aagaagaaac ttttatatat cacgtccaca atgccggtaa
     8101 aatgccagca tatacaatcc acataatcca acaagtgcat acacaactcg tgacagccag
     8161 ctcatatctc caaacaaaaa tgcaacaaga tcaaatcgga agaatccgat caatccccaa
     8221 tttatagcac caatgatcgt aagcgttaaa gctgtacaat ccaaacattt atttcccata
     8281 ataaaatcaa acctccttga tgtgaaatgt aaagaattcc agtatagtat ttacaaaaaa
     8341 aaggatttta tgtacaaggt attaaaaacc ttaaaataca tttacgttta aaaaaaaaga
     8401 gagtataata ctcatcaaag accatgtctg actaccgtga aggtaaagga ggaacaaagg
     8461 catgaaagtt atttttttag acgtagacgg agtattgaat tcacagcaat tattcgagaa
     8521 atgcgaagat gatcagctga tttctgtaga tgaggataac attaagaatt taaaaacaat
     8581 cgtagatgca acaggagcaa agatagtatt atcatcatcc tggagatacg gatgggcaga
     8641 acacagtgat gctgtgcagg actggtgtca gattctagtt gacatcttag cgaaatacga
     8701 cctaaagatc atagataaga cagaatacct aagcagtgga agaagagaag atgagatcaa
     8761 agattggctg gataagtgtg aagagaagat tgaaggtttt gtcatattag atgatggtgc
     8821 atatgaatgg catagacacg gatttgacaa acatctggtc aagacagatt tttgtacagg
     8881 tggattaaga gaagaagatg cagacaaagc aattaagatc ttaaataaga aaagattatt
     8941 cagctttttc aaaaaatact aaaggagagt taacagatgg atataaataa agtgacaaca
     9001 gcaatgatcg attactatca aggtcagcca aagagaatcc agcatttctt gnnnnnnnnn
     9061 naagtacacg cgtatgcaaa gctgatcgga gaacaggaag gattagataa ggaaatttta
     9121 gacatcctgg aagtggctgc attaacacat gatatcggta tcaaaataag cgaagaaaaa
     9181 tacaacagta gtgcaggaaa ataccaagag gttgaaggac cggcagttgc acagcagatg
     9241 ctggaagact tacaatatga caaagcaaaa acagacagag tctgctattt gatcggacat
     9301 caccatacat atgatcagat tgacggtatt gattatcaga ttcttgtaga agcagatttt
     9361 ttagtaaatc ttgcagaaga acaaagttct agagagacaa ttgagtccgt aaaaggcaag
     9421 atttttaaga caaaaacagg aatctggctg ataaataaaa ttttttgaaa aaaaagcttg
     9481 ccaagagtcg aggaatatga taaagtatag aagtcgcaga aatgagacaa caacttaact
     9541 gcccagatag ctcagtcggt agagcagagg actgaaaatc ctcgtgtcgc tggttcgatt
     9601 ccggctctgg gcattgtatg cgggtgtagt tcaatggtag aactccagcc ttccaagctg
     9661 atcacgtggg t
//
LOCUS       DAOMUE010000040         9656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1737_length_9656_cov_2.231747, whole genome shotgun sequence.
ACCESSION   DAOMUE010000040 DAOMUE010000000
VERSION     DAOMUE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1737_length_9656_cov_2.231747"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLC71_03295"
     CDS             <1..241
                     /locus_tag="PLC71_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRM01703.1"
                     /translation="QETDLIKKIEQKENIKRIYEELEKMEMNKEKKTLIMRYGLYGRE
                     PMTQKEVAKKLHISRSYVSRIEKKALMLLKSKIKT"
     gene            459..1202
                     /locus_tag="PLC71_03300"
     CDS             459..1202
                     /locus_tag="PLC71_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HRM01704.1"
                     /translation="MDAMKIANSIPMWLACALPVAFVILQAVLFARGAYKSGKKLGLN
                     DKQMKKAMKSSAVTSIGPSIVVLSAMLSLLVSVGGPIGWMRLSMIGSVMFESIAAGLG
                     TSAVGVQLGTDTLTPEALGMAVWTMILCSIGWALFATFSANKMDKIEKKVSRGNTGTL
                     TTIASCAIIGVFSAMCASHLSKPFYSMMMKADQITMSGAWKNALACVLGAVIMFVLTK
                     VANKKEIGWLKEWSLTITILGAMIITALV"
     gene            1219..1911
                     /locus_tag="PLC71_03305"
     CDS             1219..1911
                     /locus_tag="PLC71_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01705.1"
                     /translation="MNNQDYYNKEYVPQVNKIGKITGYLGVLLSFTPALVLAVVYGIL
                     PKPAALLTAFISGASAFGVLWFVEPISYFPVVGAAGTYMAFLSGNISNMRIPCASMAQ
                     VAADVEPGTEKGSVVATLGMAVSIVINVSVLTIGAILGTSVLSMLPDTIKAALNYLLP
                     ALFGALLVQFGMKMKKHSVIMVVFAIILYFMIGMGYFNWLPGASNWLGTLGCVFVSIA
                     VGMATLKNTTKE"
     gene            1927..3294
                     /locus_tag="PLC71_03310"
     CDS             1927..3294
                     /locus_tag="PLC71_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRM01706.1"
                     /translation="MIKQDVYDEVQGLEPLCQEICHTLWNHPELSGNERESANFLRKI
                     LSEEGFTIENNEHLEHAFIAEYGKGKPVIAILGEYDALPGLSQKADIKKEMVSEDGVG
                     HGCGHHMLGAAATTAAIAIKRFLEKGKQKGTVRFYGCPEEELLSGKVKMAYHHMFDGC
                     DAAVSWHPSDTNMAHDQAYLANASAHFYFHGVSSHAAFAPEQGRSALDAVELMSVGAN
                     YLREHVIDRTRIHYSTDSGGFAPNIVPDQASAWYFVRAPHMKDVKSTMERLKKVAQGA
                     AMMTETTVDIEMGCGCCELSTNEAFADLAYANLIEADGPKYTKEELRFAKELQKTVEQ
                     GIIEKGKNLHQVYDEAMILGVSPRDKWKEAELSASSDTGDVSRIMPTCLFTTACWPIG
                     CSPHTWQATASGGTSIGDKGALYASKVAAGIVYDLFTKPQVLAKITKEFRERNKEPYT
                     PMYEE"
     gene            3422..5068
                     /locus_tag="PLC71_03315"
     CDS             3422..5068
                     /locus_tag="PLC71_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HRM01707.1"
                     /translation="MGIAVKELLTLEYFKDYHVIAGKSGLHKEIQGTTLLEAPDALRW
                     AKGKELVLSSGYVLAQEPDCLEEAFKSGSMQGTSAMMIKRGRYLDEIPQRLIDLFDQY
                     EIPLISMPFSVPWMELMSQINTAVMNRTIRRFKVHSNNHLQVSDQSYKVQKINKILRA
                     VEVEMKFPAFIYDLAEEKSYYSSPDFKRITESFGLSESDYWEPSMPYTKHTLCDYINM
                     ARIRLINPGNLEGPRVSWIIIPIVMEDIVQAYFVVMESREFIDYYDEFAIRIAFLTLQ
                     GVYEQIMIAENMGNIGFENFIHLALDYTEKDAKKLFYQANIQGISVNTAYQYIVFHQC
                     NEGYNARNERNTFLEAFQQSKLGKIGKIAFLDENDGLILLEAEKIHNSIPWTKDTTTE
                     LLKEFQRYIGLKFADMRLEFGICQEEKTLLEVRECVKKCQKVLKMGSIIFPEKDIWEY
                     EMLGPLTWIEIPENELKEMLRKYREMMEEEKNIELLKTLKIYLENNMNYSVTAEKMYV
                     HINTIRKRIDKVNDLVKIDWEDHVDRLKMEILLQFLGLEE"
     gene            5133..5774
                     /locus_tag="PLC71_03320"
     CDS             5133..5774
                     /locus_tag="PLC71_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM01708.1"
                     /translation="MEMHAYSEDYLLTAQRILGDMLDYAVNEYEFDPDEFYKMFLVSD
                     VSRQFQEGNPTYIAGKNGCEIVKEVIRSAGLIMEEIPDEMYLDKSPEYWAGWALAYYQ
                     WYTARPFMKIYKVVTIEDLLKMYSVYHEMDIMKFVEAINEKWDQYYTETNLKRLRKIA
                     GLSQRELADLSGVALRQIQLFEQKKRNINHTRAIDVLKIGKVLGCKSEDLLEI"
     gene            5933..6304
                     /locus_tag="PLC71_03325"
     CDS             5933..6304
                     /locus_tag="PLC71_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRM01709.1"
                     /translation="MEKLTNLEEMIMKCIWDYGEEIPFLQIGKELKERYGKEYKRTSI
                     RTYLFRLEDKEYLKVDKRGRNAYVYPIISEKEYRKEKAEDILDNWFDGSAKELFTALS
                     EKIPKSEKERLKGVLDDMDFI"
     gene            6285..7739
                     /locus_tag="PLC71_03330"
     CDS             6285..7739
                     /locus_tag="PLC71_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRM01710.1"
                     /translation="MIWILYKNMLMLFSAVLLNFILGTIIYIIWKLLGRLAESKGYVE
                     INYWIWKIVLLAFLCPVGIIVLFGIKKKGLYGFDFWYANKIRILLTVLGIVWFIGSVI
                     RAFIYMKKMHQMHKMLQKGRKCQPEIIKKKQQICCELGQKNNIDIVKHKGIKIPMAYG
                     IFHRRIVLPEIEYSEEEIDIILHHELIHHKHHDLLWKAIFMMVHITYWFHPGMKDLIR
                     QLDQWGEAYCDRTASYYIESMKTYFNVIIDIATEEKECNTYCMGLCENSELLVLRMKR
                     MQSYLNQKPLKRITAVSLILIILGISSITVAASTIGFAKGYVYVADQTMDEESVIETE
                     KVIDYKIDRKEKIRSQKREGIEVIKQGAIIPDVNDAGILDLKLKAKERMETKKKYIDK
                     KKGKEIEISVSTGLEEDHVSKKIAIGIIEPNGKERYIEEGRNLWHGFKINKSGDYVIF
                     IENYGEKDVDISGYCSVNPYITKEEDKKFEESNN"
     gene            7720..8157
                     /locus_tag="PLC71_03335"
     CDS             7720..8157
                     /locus_tag="PLC71_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01711.1"
                     /translation="MKKVIISICIMVSLMGVFVNVNASEDQEVITSNQVLEDGFLEET
                     EEEEFLNFSTKVNVDWTVKPQILKRSKGFTKKAGSIIYINLKIFPHKKVRIGVIKGGK
                     VKKYYEVTTGLEKEIPVKDTDTYSVFVQNMTNSTINAKGSYIK"
     gene            8337..9428
                     /locus_tag="PLC71_03340"
     CDS             8337..9428
                     /locus_tag="PLC71_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRM01712.1"
                     /translation="MRKFRKYTATALVAMMVAGMTMSGYQPADVSASQESEYIIVTKD
                     KKSMKTVESKYNTMIDTQEQNTDFLKDENVLVSKMTAKEADIVNKDQKVVSVEKNTMV
                     NASETESTTAAKEDQGTDSQWNLKSIHVDKAKDTATDEKVKVALLDSGIDYQEGLNVK
                     ERVNLIEDDNEFSPMFEDSSNHGTSIASLIVSNNGKVKGINKNVELYSARILDENKQA
                     PISRVVEGIYWAIDKKVNIISISFGTNQYSEALKQAIDEANAKGILVIAAAGNQGENG
                     TDNVEYPAAFENVVAVGSVDSKAEVSDFSSTGKEVDVVAPGEAVRATGAFGETMVTSG
                     TSMAVPHVVGAVFCGRKIQVNQKILSKIC"
BASE COUNT         3685 a         1373 c         2083 g         2515 t
ORIGIN      
        1 acaggaaacg gacttgatca aaaagatcga gcagaaagaa aatatcaaaa gaatctatga
       61 agaattagag aaaatggaga tgaataagga gaagaaaaca ctgatcatgc gttatggact
      121 ttatggaaga gaaccaatga cacaaaaaga agtagcaaaa aaacttcata tttcaagatc
      181 ctatgtaagc aggattgaaa agaaagcact gatgttatta aaaagtaaaa taaagacatg
      241 acacacttat atcttagata ttaagagaaa atggtagttt ttcttttatt aatttaataa
      301 agtgaaaaaa tcttcactat acaaagaaaa taatcataaa aatcttcgaa aacaaagatg
      361 ccatatcaaa aagtgaaaaa tttttcatat catttcaccc acaaaacagg taatataatt
      421 gcatacaaaa cacacggacc gataaggagg ataaaaatat ggacgcaatg aaaatcgcaa
      481 atagtatccc aatgtggctt gcctgtgcac ttccagtggc atttgtcatc ttacaggcag
      541 tcttattcgc aaggggtgct tacaaatcag gaaagaaact aggacttaac gataaacaga
      601 tgaaaaaagc aatgaaaagc agtgcggtaa catcgatcgg accatccatc gttgtattaa
      661 gtgcgatgct ttctctgtta gtatctgtag ggggaccaat cggatggatg agattatcta
      721 tgatcggttc cgttatgttt gaatctatcg cagcaggact tggaacatcc gctgtcgggg
      781 tacagcttgg aacggacaca ttaacaccag aagctttagg aatggcagtc tggaccatga
      841 tcctttgctc tatcggatgg gcgttattcg caacattctc tgcaaataag atggataaga
      901 tcgaaaagaa agtttccaga ggaaatacag gaacactgac aacgattgct tcttgtgcga
      961 tcatcggtgt attctcagca atgtgtgcaa gccatttatc caaaccattt tactctatga
     1021 tgatgaaagc agaccagatc acaatgagcg gagcatggaa gaatgcctta gcatgtgtgc
     1081 ttggagccgt gatcatgttt gtacttacca aagttgcaaa taaaaaagag atcggatggt
     1141 taaaagaatg gtccctgacg atcacaattc ttggagctat gatcattaca gcattagttt
     1201 aagcaggagg aagatacgat gaacaatcag gattattata ataaagaata tgtaccacaa
     1261 gtcaataaga tcggtaagat cacaggatac cttggagttc ttctttcctt tactccagca
     1321 cttgtattag ctgttgtcta tggaatctta ccaaaaccag ctgcattatt aaccgcattt
     1381 atcagcggag ccagtgcctt tggagtgtta tggtttgtag aaccgatctc ttactttcca
     1441 gtcgtcggag ccgctggaac ttatatggca ttcttatcag gaaatatctc taacatgaga
     1501 atcccatgtg caagtatggc acaggtggca gcagatgtag aaccaggaac agaaaaagga
     1561 tctgttgttg caacccttgg aatggcagta tctatcgtga tcaacgtatc tgttcttaca
     1621 attggagcaa ttcttggaac ttctgtatta tccatgcttc cagatacgat caaagccgca
     1681 ttaaactact tgctgccagc tttatttgga gctttattag tacaatttgg aatgaaaatg
     1741 aaaaaacaca gcgtcatcat ggttgtattt gcgatcatct tatattttat gatcggaatg
     1801 ggatacttta actggttgcc aggggcatcc aactggcttg gaaccttagg atgtgtcttc
     1861 gtaagtattg ctgttggaat ggcgacatta aagaatacaa caaaggaata gaaagcaggg
     1921 gataaaatga tcaaacagga cgtttatgat gaggtgcaag ggttagagcc gttgtgtcag
     1981 gaaatctgtc atactttatg gaatcaccca gaattatctg gaaatgaaag agaatctgcc
     2041 aatttcttaa gaaaaatctt gagtgaagaa ggattcacga ttgagaacaa tgaacattta
     2101 gaacatgcgt ttattgcaga atacggaaaa gggaaaccag ttatcgcaat tcttggagaa
     2161 tacgatgcac ttcctggatt atcacaaaaa gcagatatta aaaaagaaat ggtatccgaa
     2221 gatggtgtcg gacacggatg tggacaccat atgttaggtg ccgcagctac aactgcagct
     2281 attgcaatca aacgtttctt agaaaaaggg aaacaaaaag gaacagtcag attctatgga
     2341 tgcccagaag aagaactgtt aagtggaaag gtaaagatgg catatcatca tatgtttgat
     2401 ggatgtgatg cagcagtgag ctggcatcca tctgatacaa acatggcaca tgatcaagca
     2461 tatcttgcga atgcatctgc acatttttat ttccatggag tatcatcaca tgcagcattt
     2521 gctccggaac agggaagaag tgctttagac gccgtggaat taatgagtgt tggagcaaac
     2581 tatttaagag aacatgtcat tgaccgcaca agaattcatt attctacaga cagcggtgga
     2641 tttgcaccca atatcgtgcc agaccaagca agtgcatggt attttgtcag agcaccacat
     2701 atgaaggatg tcaaaagcac aatggaacgc ttaaagaaag tagcccaagg tgcagcgatg
     2761 atgacagaga cgacagtgga tattgaaatg ggatgtggat gctgtgagtt atccacaaac
     2821 gaagcatttg cagaccttgc atatgcgaac ctgattgaag cagatggacc caaatacaca
     2881 aaagaagaac tacgatttgc aaaagagttg cagaaaaccg tggaacaagg tatcatagag
     2941 aaaggaaaaa atcttcacca agtttatgat gaagcaatga tcttaggtgt ttcgccaaga
     3001 gataaatgga aagaagcaga actaagtgca tcttctgata caggagatgt cagcaggatc
     3061 atgccaacct gtctgtttac aactgcatgc tggccaatcg gatgctctcc acatacatgg
     3121 caggcaaccg caagcggagg gacttccatc ggagataaag gagccttata tgcatctaaa
     3181 gtggcagcag gaatcgtata tgatctcttt acaaaaccac aggtcttagc gaagattaca
     3241 aaagaattta gggaacgaaa taaagaacca tacacaccga tgtatgaaga atgatcgata
     3301 tgcaaagaag catccggttc ctatgagcag taggaatcgg attttttctt atatatatgg
     3361 atgtttgata gaagatgaat gatttataga aaaaacacga taaaaatgga ggaaataaag
     3421 aatgggaatc gctgtcaagg aactactgac actagaatac tttaaagact atcacgtgat
     3481 cgcagggaaa tcaggcttac acaaagagat tcagggaaca accctccttg aagcacccga
     3541 tgccctgcgc tgggcaaaag gaaaagagct ggtcctttcc tcaggatatg tattagctca
     3601 ggaaccagac tgcctcgaag aagcatttaa gtcaggaagc atgcagggaa cctctgccat
     3661 gatgatcaaa cgaggccgct atcttgacga gataccacag cgattaatag atttgtttga
     3721 tcaatacgag attccactga tcagtatgcc attttctgtt ccatggatgg aattgatgag
     3781 tcagatcaat accgcagtga tgaaccgaac gatccgaaga ttcaaagtcc acagcaataa
     3841 tcatctgcaa gtatcggatc aatcctacaa agtccagaag atcaataaga ttctaagagc
     3901 cgtagaagtc gagatgaaat ttccggcatt tatctatgat cttgccgaag aaaaaagcta
     3961 ctacagttcc ccggatttta aacgaatcac agaatccttc ggattatccg aaagcgatta
     4021 ttgggaacca tcaatgccct atacgaaaca taccttatgc gattacatca atatggcaag
     4081 gatccgcctg atcaatcctg gcaatttaga aggaccacgt gtcagctgga tcattattcc
     4141 gatcgtcatg gaagatatcg tgcaggcata ttttgtcgta atggaatcca gagaatttat
     4201 cgattactac gatgaatttg ccattcgtat cgcgttttta acattgcaag gagtctatga
     4261 acagatcatg atcgcagaga atatgggaaa tatcggattt gagaacttca ttcatttagc
     4321 cctcgattac acagaaaaag atgccaagaa actcttttat caggcaaata tccaaggaat
     4381 ttccgtaaat acagcttatc aatacattgt atttcaccaa tgcaatgaag gatacaacgc
     4441 acgaaatgaa agaaacacat ttttggaagc cttccagcaa agcaagttag ggaagatcgg
     4501 aaagattgcc tttctggatg aaaatgatgg cttgatctta ttagaagcag aaaagatcca
     4561 caacagcata ccctggacca aagacacaac aacggaatta ttaaaagaat tccagagata
     4621 catcggatta aaatttgccg atatgagact ggaatttgga atctgtcagg aagaaaagac
     4681 actgttagaa gtcagagaat gtgtcaagaa atgccagaaa gtattaaaga tgggaagcat
     4741 tatcttccct gaaaaggata tctgggaata cgaaatgtta ggaccattaa catggattga
     4801 gattcctgag aatgagttaa aagaaatgtt aaggaaatat cgggagatga tggaagaaga
     4861 gaagaatatt gagttgttaa agacattaaa gatttatctg gaaaataata tgaactacag
     4921 cgtgaccgcg gagaagatgt atgtgcatat taatacgata agaaagagaa ttgataaagt
     4981 gaatgatctg gtgaagattg attgggagga tcatgtggat cgattgaaga tggagatttt
     5041 attgcagttt ttgggattgg aggagtgaaa atattaattt ttgttgagga agtatcgcac
     5101 ttgcgatata ataaaaataa gaggaaataa aaatggagat gcacgcttat tctgaggact
     5161 atttgctaac agcacaaaga attctaggag atatgttaga ctatgcagta aatgaatatg
     5221 aatttgaccc agacgagttt tataaaatgt ttttagtatc cgatgtatcc agacaatttc
     5281 aagaaggaaa tccaacatat atagcaggaa aaaatggctg tgaaatagta aaagaagtaa
     5341 taagatcagc aggacttata atggaagaaa ttccagatga aatgtatttg gacaaatctc
     5401 cagaatattg ggctggctgg gcattggcgt actatcaatg gtatacagca agaccgttta
     5461 tgaagatata caaagtagta acgatagaag atcttctaaa aatgtattcc gtatatcatg
     5521 aaatggatat tatgaaattc gtagaagcaa tcaatgaaaa atgggatcaa tattatacag
     5581 aaacaaattt aaaacgttta cgtaaaattg caggattgtc acaaagagaa cttgcagatt
     5641 tgtccggtgt tgcactgaga cagattcaat tatttgagca aaaaaaacga aatattaatc
     5701 acacaagagc gattgatgta ttgaagattg ggaaagtttt aggatgtaaa tcggaagact
     5761 tgttagagat ataaagtaaa aagggtagat gtcattttgg catctatctt ttttattgat
     5821 ataattagca aatcaataaa gttatacgaa cgactaaaaa ataagttgaa tggatgatta
     5881 aaatatgtta atataaaaat aactaaaaaa gttatttaaa gaggtggaga atatggaaaa
     5941 attaacaaat ttagaagaaa tgatcatgaa atgtatttgg gattacggag aagagattcc
     6001 atttttacaa attggaaaag agttaaaaga acggtatggc aaggaataca agagaacaag
     6061 tatacgaact tatctatttc gtttagaaga taaagaatat ttgaaagtag ataaaagggg
     6121 aagaaatgcc tatgtttatc cgattatttc ggaaaaagaa tatcgaaaag aaaaagcaga
     6181 agatatactg gacaattggt ttgatggatc agcaaaagaa ttatttactg cattaagtga
     6241 aaagattcca aaaagcgaaa aagaaaggct aaaaggtgta ttagatgata tggattttat
     6301 ataaaaatat gctgatgctg ttttcagcag tactgcttaa ttttatatta ggaacgatca
     6361 tatatattat ttggaagtta cttggaagac ttgcagaaag caagggatat gtggagatta
     6421 attattggat atggaagatc gtactattag catttctgtg tccagtgggg attatagtac
     6481 tttttggaat aaagaagaaa ggattgtatg gttttgattt ttggtatgcg aataaaatta
     6541 gaattctgtt aacagtatta ggaatcgtgt ggtttattgg aagtgtgata agggctttca
     6601 tatatatgaa aaagatgcat cagatgcata aaatgttaca gaaaggaaga aaatgtcagc
     6661 ctgagatcat caagaaaaaa cagcagattt gttgtgaatt aggacaaaaa aataatattg
     6721 atattgtaaa acacaaagga ataaaaattc caatggcata tgggattttt catcgaagaa
     6781 tcgttttgcc tgaaattgaa tatagtgaag aagaaataga tattattttg caccatgaat
     6841 tgattcatca taaacatcat gatctgttat ggaaagcaat ctttatgatg gtccatatta
     6901 cttattggtt tcatccagga atgaaagatt tgataagaca gttagaccaa tggggagaag
     6961 catattgtga tagaacagct agctattata tagaaagcat gaagacttat tttaacgtga
     7021 tcatagatat agcaacggaa gaaaaggaat gtaatacata ttgcatggga ttatgcgaaa
     7081 atagtgaact tttggttctt cgaatgaaaa gaatgcagtc ttatttaaat caaaagccat
     7141 taaaaaggat aacagcagtt agtttgatat tgatcatatt gggtataagt tcgataactg
     7201 tggctgcatc tacgattgga tttgcaaaag gatatgttta tgttgcagat cagacaatgg
     7261 atgaggaatc agtaatagaa acagagaaag ttatagatta taaaatagac agaaaagaga
     7321 aaatacggag tcaaaaaaga gaaggaatag aagtgataaa acaaggagct ataattccag
     7381 atgttaatga tgcgggaatt cttgacttaa aacttaaggc aaaagaaaga atggaaacga
     7441 aaaagaaata tatagataaa aagaaaggga aagaaatcga aatttcagtt agcacagggt
     7501 tagaagagga tcatgttagt aagaaaatag caataggaat tattgaacca aatggaaagg
     7561 aaagatatat tgaagaagga agaaatttat ggcatggttt taaaataaac aaaagtggag
     7621 attacgtaat ttttattgaa aattatggag aaaaggatgt ggatatttca ggttattgtt
     7681 cagttaatcc atatataacg aaagaggagg ataagaaatt tgaagaaagt aataattagc
     7741 atatgtataa tggtgagttt aatgggggtt tttgtaaatg taaatgcatc agaagatcag
     7801 gaagtgataa cttcaaacca ggtgttggaa gatggatttc tagaagaaac tgaagaagaa
     7861 gaatttttaa atttttcaac aaaagtaaat gtggattgga ctgtgaaacc tcaaatactt
     7921 aaaagatcga aaggttttac aaaaaaagca ggtagtataa tctatattaa tttaaaaatt
     7981 tttccacata aaaaagttcg aattggagta atcaaaggtg gaaaagttaa gaaatattat
     8041 gaagttacta cagggttgga aaaagaaata ccagtaaaag acacagatac atatagtgtt
     8101 tttgtacaaa atatgacaaa ttcaacgatt aatgctaagg ggagctatat taagtaatgt
     8161 ataaaataag tagaaggagg tgatgtcata tgaacaatta aaaaatccat gtaatacaaa
     8221 agacgggaat cttcaacaat ctgtataata tacagataca cgtatcacat ggattcttat
     8281 attatagcaa aatacatcag aaaatcaaga ttttactaca gtataggagg aaacacatga
     8341 gaaagtttag aaaatacaca gccacagcat tagtagcaat gatggtggca gggatgacaa
     8401 tgtctggata tcagccagca gatgtttcgg caagtcagga aagcgaatat atcattgtaa
     8461 caaaagataa aaaatctatg aaaacagtag aaagcaaata caatacaatg atagatactc
     8521 aagaacaaaa tacagatttt ctaaaagatg agaatgtatt agtatcaaaa atgacagcaa
     8581 aagaagctga tatcgtaaac aaagatcaga aagttgtatc tgtagaaaag aacacaatgg
     8641 tcaatgcatc ggaaacagaa agtacaacag cagcaaaaga agatcaggga acagacagtc
     8701 aatggaatct gaaaagtatt catgtagata aagcaaagga tacggcaaca gatgaaaagg
     8761 taaaagtagc attattggat tcaggaattg attatcaaga aggattaaat gtaaaagaaa
     8821 gagtcaattt aatagaagat gataatgaat tttctccaat gtttgaagac agttccaatc
     8881 atggaacatc cattgcaagc ttaattgtca gtaataatgg aaaagtaaaa ggaatcaata
     8941 aaaatgttga attatattca gcaaggattc tggatgaaaa taaacaggca ccaatcagca
     9001 gagtcgtaga aggaatttat tgggccattg ataagaaagt aaatattatc agcatcagtt
     9061 ttggaacaaa tcaatattca gaagccttaa aacaggcaat tgatgaagcc aatgcaaaag
     9121 gaatcttagt aatcgcagca gcaggaaatc aaggagagaa tggaacagat aatgttgaat
     9181 atccagcagc atttgaaaat gttgtagcag tcggttccgt agattctaaa gcagaagtaa
     9241 gcgatttcag ttctacagga aaagaagttg atgtagtagc accaggagaa gcagtaagag
     9301 caacaggtgc atttggtgaa acaatggtta catctggaac aagtatggca gttcctcatg
     9361 ttgttggtgc agtcttctgt ggcagaaaga tacaagtaaa tcaaaagatt ttatcaaaaa
     9421 tctgttagaa gaaagtgcaa gaaatgtagg agacaaagaa tcctatggaa atggactgat
     9481 cgactatgaa tatgcagaga agcaatacac aaaagcagaa gaacaatatg agcagggaca
     9541 ggatattgaa ctaaaggata atacaaaaac aattaaaact tataataatt cctctgatga
     9601 gaataaagtt tctggaacat ggtcagcggc gggacaccag aaatatttaa ctgaca
//
LOCUS       DAOMUE010000041         9637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1744_length_9637_cov_3.443018, whole genome shotgun sequence.
ACCESSION   DAOMUE010000041 DAOMUE010000000
VERSION     DAOMUE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9637
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1744_length_9637_cov_3.443018"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /gene="ftsH"
                     /locus_tag="PLC71_03345"
     CDS             complement(<1..1407)
                     /gene="ftsH"
                     /locus_tag="PLC71_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRM01713.1"
                     /translation="MNPKKSNSGFLVVLLILVIGAYYFISVFQPGSGDYTYQQFMKDV
                     KSDKVTAVTIKQNKEVPTGTITAEFGKDDYKSCEVTDVNQAINDIKKADSSLANKISI
                     KGIDHSGDMISNLIGVVLPIGVVIFFMVMMMRQNGGGGGKMMDFGKSRAKLANPNGKK
                     VTFNDVAGLTEEKEELEEVVEFLKNPGRFIKIGARIPKGVLLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSDFVEMFVGVGAARVRDLFAEAKKHSPCIVFIDEIDAVARRRGSGL
                     GGGHDEREQTLNQMLVEMDGFGVNEGIIMIAATNRVDILDPAILRPGRFDRKVGVGRP
                     DVKGREDILKIHCRQKPLGDDVDLHEIARTTAGFVGADLENLMNEAAIQAARDDRAYI
                     MKEDIDKSFIKVGIGTEKKSRVVPESERRITAYHESGHAILFHLLPDVGPVYTVSIIP
                     TGTGAAGYTMPLPENDNVF"
     gene            complement(1423..1944)
                     /gene="hpt"
                     /locus_tag="PLC71_03350"
     CDS             complement(1423..1944)
                     /gene="hpt"
                     /locus_tag="PLC71_03350"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRM01714.1"
                     /translation="MENIRVMISEEDVNKRIAEMGAQISEDYAGKEIHLIAILKGSVF
                     FACELAKRITIPVTIDFMSVSSYGNSRESSGIVKIAKDLDEPILDKEVLVVEDIIDSG
                     KTLNHLKPMLESRNPASLHLCTLLSKPDRREVEVPVRYIGFEVPDEFVVGYGLDYAQK
                     YRNLPYIGVAEEE"
     gene            complement(1868..3310)
                     /gene="tilS"
                     /locus_tag="PLC71_03355"
     CDS             complement(1868..3310)
                     /gene="tilS"
                     /locus_tag="PLC71_03355"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRM01715.1"
                     /translation="MKESAKNDKVNYMNKVKELIKEKNLIQNGDHIIIGVSGGADSVY
                     LLLILNELRESMNLTITAVHINHMIRREAVDDQKFVQDLCGQLSIPCKIFEIDVKQIA
                     KREKMSLEEAGRKARYDAFAKTMKKYNADKIAIAHHQNDQAETFLYRVVRGTGIYGAG
                     AMREKDGNLIRPLLCVKKEEIVKYLKDAGQAWVEDASNQDDAFARNQIRNQVIPRLEM
                     INEQAVEHIGWLCEDIKEVTTYLTDQIEESFLRCTKPIGQGFEINCDQLLLENHWIQK
                     QVLKKVLEETAGKKKDLERRHIEDLLTLVENGTGKRISLPYNMVAEKNYQYIKVQKGN
                     ISDKQEMTGKILCEEVTDLTNIVENDCIKIIDYDRIETGVQLRCRKPGDFFTFGKDQK
                     RKSLSRYFIDEKIPRQLREEIPLVADGSHIVWIVGRRISEYYKIKESTKRYLKLEFKR
                     EGDDSNGEYQSNDFRGRCKQTNRGDGSADQ"
     gene            complement(3307..4713)
                     /locus_tag="PLC71_03360"
     CDS             complement(3307..4713)
                     /locus_tag="PLC71_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRM01716.1"
                     /translation="MEILRKKEFEEDRLFQNFHEMILYHMRQMQQCLTTAGKMFCDHN
                     DEEETCEKRGRDVVESVILKECVGCCENQKCQFTLADKDRLGQIIEKQGGLSYADLKR
                     CHPCKNGQDFIEEANQIYERELFLRAMRNQMKQLRKIVGEQYIRAGNTLGDFFQGKVS
                     LTADNKKLYEKITKGFAKSQLRLKEIYFYDNPEKGKQIYLYLKKKKGREISTRQAAVL
                     LSSMTMEKMKPLPDQKKMIGNYYEIYGFTPEEKFHVIAGIHTSPMIAGDENGDSFSMG
                     KVREGSFVSMISDGMGTGQEAKKESRKIIEVMEELLGAGINESQSIQLLRSMMILQPE
                     KEKYATLDFFQLDLFAGIGTFFKIGACPCLLKRKNDVEIIQVDTIPVNLLLHTEKIPI
                     YRKKMESEDILVMLSDGAFDGFSQNGLEMAARYLKEMDVVRPQAIADGLYEQITTNKE
                     FEKRDDMTIMVLGIWDRY"
     gene            complement(4877..5731)
                     /gene="nadC"
                     /locus_tag="PLC71_03365"
     CDS             complement(4877..5731)
                     /gene="nadC"
                     /locus_tag="PLC71_03365"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRM01717.1"
                     /translation="MSGVTMRINIDEYLMNALKEDITQEDVSTNAVMPEPKQGEVNLI
                     CKQDGVICGLEVFERVFKLLDETVVFETELKDGDKVTKGQLMGVVKGDIRALLSGERV
                     ALNYLQRMSGIATMTSELSKELEGYKTKLLDTRKTTPNMRPFEKYAVTVGGGHNHRYN
                     LSDGVMLKDNHIGAAGGIKEAVAMAKDYAPFVRKIEVETENLDMVKEAVEAGADIIML
                     DNMDDETMKEAVKLIDGRAETECSGNVTKERLKTIREIGVDYVSCGALTHSAPIMDIS
                     LKNLHPVE"
     gene            complement(5706..7007)
                     /locus_tag="PLC71_03370"
     CDS             complement(5706..7007)
                     /locus_tag="PLC71_03370"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRM01718.1"
                     /translation="MKTADVIIVGSGASGLFNALFLPKDYKVIVITKKEVEESDSFLA
                     QGGISALKNPEDYDSYFEDTMKAGHYENDKGAVEKMIKESPQIIQDLKDFGVRFDLKD
                     GEIEFTREGGHSTFRILHHEDLTGKEITSHLYEEAKKRDNITIMENCTMIDIIEKDGE
                     CKGIVYKDADGNLDTIEAPDTVLATGGLGGLFKHSTNFRHLTADSLAICLRHNVELEN
                     INYIQIHPTTFYSKKPGRRFLISESVRGEGAYLLNKDGERFTDELKPRDVVTGEICKQ
                     MKKDGSDHVYLSVTHLDGERIKHRFPNIYKQCLDEGYDMTKEPIPVTPAQHYFMGGIK
                     VNLDSKTSMEHLYAVGETSCNGVHGKNRLASNSLLEALVFAKAAAKDITEHPSKAETV
                     EVDLSKYQNEEQREKENEQLVLDEIKRKDRSFYDQWCNDEN"
     gene            complement(7042..7950)
                     /gene="nadA"
                     /locus_tag="PLC71_03375"
     CDS             complement(7042..7950)
                     /gene="nadA"
                     /locus_tag="PLC71_03375"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRM01719.1"
                     /translation="MENLKKEIEQLKQEKNAVLLAHYYVDDAVQEIADYVGDSYYLAK
                     VALKESKDVICFAGVNFMGESAKILNPDRTVIMPDQKADCPMAHMVDVEKIEEMRKKY
                     DDLAVVCYINSTAEIKAHSDVCVTSSNALKVVTALPQKNIFFVPDENLGRYIGSKLPE
                     KNFIYNDGFCHVHRSITAENVKKAKEVHPEAEVLVHPECTPDVVALGDYVGSTSGIID
                     YATASDKDVFIICTEMGVLYELKQKNPGKTFYSVGHRQFCPNMKRISLENVRDTLRDM
                     KNQVELPEELRLNAKKALDEMLRIAQ"
     gene            complement(8170..9486)
                     /locus_tag="PLC71_03380"
     CDS             complement(8170..9486)
                     /locus_tag="PLC71_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HRM01720.1"
                     /translation="MKLQESERTEFKSIVVTDIKKEIIAFANCDGGKIYIGIEDDGTV
                     IGVEDSDSVALQVSNMVRDAIKPDLTMFLHYETKEIEQKQVVIIDIQRGTERPYYIAK
                     KGLRPEGVYVRQGYSSVPATDTAIRKMIKETDGDHFEDMRSLDQELTFCETKKEFQKR
                     NITLEQPQMKTLGIINQDGIYTNLALLLSDQCVHTIKTAVFEGKNQNIFKDRKEFTGS
                     LFAQMEAVYDYIDFRNQTHSTFEKLYRIDNRDYPEVAVREALLNCLVHRDYGYSSSIL
                     ISMYEDRLEFTSIGGLVTGVTLEDVMMGISVCRNKRLANVFYRLELIEAYGTGIQKIM
                     NAYQDQFIKPEIKVSNNVFKIILPNTNAQAELREELQYHTHIQPEDEETQIMQMIDQE
                     KRIQRKQVQERLGISQTTSGRILKQMVKKGLIMQRGRGKNTCYVKK"
BASE COUNT         2442 a         2196 c         1398 g         3601 t
ORIGIN      
        1 aaagacatta tcgttctcag gaagcggcat cgtatatcca gctgctcctg ttcccgttgg
       61 aataatagat acagtgtaaa ccggacctac atctggtaac agatggaata agattgcatg
      121 tccagattca tgatatgcag tgatccttct ttcactttct ggtaccacac gactcttctt
      181 ctctgttccg attcctactt tgataaatga tttatcaata tcttctttca tgatataggc
      241 tctgtcatct cttgctgcct gaatggctgc ctcattcatc agattctcaa ggtctgctcc
      301 cacaaaacct gctgttgttc ttgcgatctc atgaagatca acatcatctc ctaatggttt
      361 ctgacgacag tgaatcttta agatatcttc tcgccctttg acatctggtc ttccaacgcc
      421 aaccttgcgg tcaaatcgtc ctggtcgtaa gatcgctgga tctaagatat ccacacggtt
      481 tgtagctgca atcatgatga ttccttcatt cactccaaaa ccgtccatct caactaacat
      541 ctggtttaat gtctgttcac gttcatcatg tccaccacca agtccagagc ctcttcgtct
      601 tgcaactgca tcgatctcat cgataaatac gatacaagga gaatgtttct tagcttctgc
      661 aaataagtca cgtacacgag ctgcaccaac accgacgaac atttccacga aatcagatcc
      721 tgaaatgctg aaaaatggta cattcgcttc tcctgcaact gcctttgcaa gtaatgtctt
      781 acctgttcct ggaggtccta caagaaggac gccctttgga attctggctc cgatctttat
      841 aaatcgtcct ggattcttta agaattccac gacttcttct aattcttctt tctcttctgt
      901 cagtcctgca acatcgttaa atgttacttt cttaccattt ggattggcaa gtttggcacg
      961 gctttttcca aaatccatca tcttgccgcc accgccacca ttctggcgca tcatcataac
     1021 catgaaaaat atgacaactc cgatcggtag tactaccccg atcagattag agatcatatc
     1081 cccactgtga tcgattccct taatactgat cttattggca agagagctgt cagctttctt
     1141 aatatcattg atcgcctgat tgacatctgt aacttcacag ctcttataat catcttttcc
     1201 aaactctgct gtgatcgtcc cagttggtac ttctttattc tgtttaatag ttaccgcagt
     1261 tactttgtca gactttacat ccttcataaa ctgctgatag gtgtaatcac ctgaacctgg
     1321 ctggaacacg ctgataaaat aatatgctcc gatcaccaag atcagcagga ctactaaaaa
     1381 tccactgtta cttttcttcg ggttcaacgt ttagtctcct ctctactctt cctctgctac
     1441 tccaatgtaa ggaaggttac gatatttctg tgcataatct agtccatatc caacaacaaa
     1501 ttcatctggt acttcaaaac caatatatct gactggtact tcaacttcac gacgatctgg
     1561 tttgcttaac aatgtacaca gatgaagtga cgcaggattt ctgctctcaa gcattggttt
     1621 taaatgattt aaagtctttc ctgagtcaat gatatcttct acaactaata cttctttatc
     1681 taagattggc tcatctaagt cttttgcgat cttaacgatt cctgaggatt ctctggaatt
     1741 tccataacta gacactgaca taaaatcaat tgttactgga atcgtgattc tctttgcaag
     1801 ttcacacgca aaaaaaacac ttccttttaa gatggcgatc agatggattt cttttcctgc
     1861 ataatcttca ctgatctgcg ctcccatctc cgcgattcgt ttgtttacat cttcctctga
     1921 aatcattact ctgatattct ccattggaat catctccttc tcttttgaac tcaagcttta
     1981 aataacgctt ggttgattct ttgatcttat aatattcgct gattcttctg ccaacgatcc
     2041 atacaatatg actgccatct gccaccaagg gtatctcttc ccgcaattgt cttggaatct
     2101 tctcatcaat aaaatacctg cttaatgatt ttctcttctg atctttgcca aatgtaaaaa
     2161 aatctcctgg cttccggcat cgtaattgta cacctgtttc tattctatca taatcaatga
     2221 ttttgataca atcattttcc acgatatttg tcaaatctgt aacctcttcg cacaaaattt
     2281 ttcctgtcat ttcttgttta tctgatatat ttcctttttg taccttaata tactgataat
     2341 ttttctcggc aaccatatta tacggaaggg aaattcgttt ccctgttcca ttttctacca
     2401 aagttaacag atcttcaata tgtcttcttt ctaaatcttt cttcttacca gctgtttctt
     2461 ccaaaacttt ttttagaacc tgtttctgga tccaatggtt ttccaataac aattgatcac
     2521 aattgatctc aaatccttgt ccaataggct ttgtacatct taaaaatgac tcttctatct
     2581 gatctgtcag atacgtcgtc acttctttga tatcttcgca cagccatcct atatgctcca
     2641 ccgcctgctc attgatcatc tcaagtcttg gaattacctg atttcggatc tgatttcttg
     2701 caaatgcatc gtcctgattg cttgcatctt ctacccaggc ctgcccagca tcttttaaat
     2761 attttacgat ttcttctttt tttacacata aaagcggacg gatcagattt ccatcttttt
     2821 ctctcatggc acccgcccca taaattccag ttcctctcac cactcgatat aaaaatgttt
     2881 ctgcctgatc attttgatga tgtgcgatcg caatcttatc agcattatat tttttcattg
     2941 ttttcgcaaa tgcatcatat ctcgcttttc ttcctgcttc ttctaatgac atcttttcac
     3001 gcttggcgat ctgttttaca tctatttcaa atattttaca cggaatcgaa agttgtccac
     3061 aaagatcctg cacaaacttc tgatcatcca cagcttctct tcgaatcatg tgattgatgt
     3121 ggactgctgt gatcgtgaga ttcattgatt ctcttaattc atttaaaata agtaacagat
     3181 atacagaatc tgcaccgcca gaaacaccga taatgatatg atctccattt tgtattaaat
     3241 ttttttcttt gataagttct tttactttat tcatgtaatt tactttatca ttctttgcag
     3301 attccttcaa tacctgtccc agattcccaa taccatgatc gtcatatcat ctcttttttc
     3361 aaattctttg tttgttgtaa tttgctcata taatccatct gcgatcgcct gtggacgtac
     3421 cacatccatt tctttcaaat atcgtgctgc catctccaaa ccattttgcg aaaatccgtc
     3481 gaaggctcca tcgcttaaca tgactaaaat atcttcactt tccatctttt tgcggtaaat
     3541 tggaattttc tcggtatgta ataagaggtt cactggtatc gtatctactt gaatgatctc
     3601 cacgtcgttt tttctcttta aaagacatgg gcaggctcca attttaaaga atgttccgat
     3661 tcctgcaaat aaatctaact gaaaaaaatc taatgtcgca tatttttctt tttctggttg
     3721 taagatcatc atggacctta aaagctgaat actttgactt tcgttaattc ctgctcctag
     3781 aagttcttcc atcacttcaa ttattttcct actttctttt ttcgcttcct gtcctgttcc
     3841 cattccatcc gagatcatag acacaaaact tccttccctt acttttccca tggaaaaact
     3901 gtctccattc tcatcccctg cgatcattgg cgaagtgtgg attcctgcga ttacatggaa
     3961 tttttcttct ggtgtaaatc cataaatctc ataataattt ccgatcatct ttttctgatc
     4021 tggcaatggc ttcattttct ccattgtcat ggaacttaac aacactgctg cttgtcttgt
     4081 gctgatttcc ctgccttttt tctttttcag atatagatag atctgttttc ctttctctgg
     4141 attatcgtag aaataaattt cttttagtct cagttggctt tttgcaaaac cttttgtgat
     4201 cttctcatat aatttcttat tatctgccgt taatgatact tttccctgaa agaaatcacc
     4261 cagtgtattt ccggctctga tatactgctc cccgacaatt tttctcagtt gtttcatctg
     4321 atttctcatt gcacgcagaa ataattcccg ttcataaatc tgatttgctt cctcgataaa
     4381 atcctgtcca ttcttgcatg gatgacatct ttttaaatct gcatagctta atccaccttg
     4441 cttttcgatg atctgcccaa gcctgtcctt atctgctaat gtaaactgac atttttgatt
     4501 ttcacagcat ccgacacatt cttttaagat cacactttca actacatctc ttccccgttt
     4561 ttcacacgtt tcttcctcat cgttatgatc acagaacatc tttcccgctg tcgtgagaca
     4621 ttgctgcatc tggcgcatat gataaaggat catttcatga aaattttgaa ataaccggtc
     4681 ttcttcgaat tctttttttc ttaagatttc catagttttc cctcctgcaa gattattata
     4741 gaggattttg tatgcaattt ctgtcgaaat aaaaaatgtt attttctgga aattattatc
     4801 aaaaaaatcc gatgttttta taagaacaat cctatgacta ttcttataaa aacatcggat
     4861 tttatatctt tattctttac tctaccggat gtaaattctt caaagaaatg tccataatag
     4921 gagctgaatg tgttaaagct ccacaggata cataatccac tccgatctca cggatcgtct
     4981 ttaggcgttc ttttgtaaca tttcctgaac actctgtttc agctcttccg tcgatcagtt
     5041 tgacagcttc tttcattgtc tcatcatcca tgttatctaa catgatgatg tctgcgcctg
     5101 cttctacggc ttcttttacc atgtcaagat tctctgtctc tacttcaatc tttctgacaa
     5161 atggtgcata gtcttttgcc atagctaccg cttctttgat tccacctgca gcaccgatat
     5221 ggttatcttt taacatgact ccatcggata agttgtagcg gtgattatgt ccaccaccaa
     5281 ccgtcactgc gtatttttca aatgggcgca tatttggtgt tgtttttctt gtatctaata
     5341 actttgtttt atatccttca agctctttgg ataactcgct tgtcattgtc gcgattccac
     5401 tcattctctg caagtagttt aatgcaactc tctctccaga taaaagggca cggatatctc
     5461 ctttgacaac tcccattagc tgtcctttgg tcactttatc tccgtctttt aattctgtct
     5521 caaaaacaac agtttcatct aaaagtttga agactctttc aaatacttct aatccacaga
     5581 tgaccccatc ctgtttacag attaaattta cttctccctg ttttggttct ggcatcactg
     5641 cgtttgtact gacatcttcc tgagtgatat cttcttttaa tgcattcatc aaatattcat
     5701 cgatgttaat tctcatcgtt acaccactga tcataaaaac ttctgtcctt tcgtttaatt
     5761 tcgtcaagaa caagctgttc attctctttc tcacgctgtt cttcgttctg atacttactt
     5821 aaatctactt cgactgtctc ggcttttgat ggatgttctg tgatgtcttt cgctgctgcc
     5881 ttggcaaata caagtgcttc caataatgaa ttacttgcca gacggttctt tccatgaacc
     5941 ccattacagc ttgtctcacc gactgcatat aaatgttcca tagatgtctt gctgtctaag
     6001 tttactttaa ttccacccat gaaatagtgc tgtgccggtg ttactggaat cggttctttt
     6061 gtcatgtcat atccttcatc caagcactgt ttgtagatgt ttgggaatct atgtttgatt
     6121 ctttctccat ctaaatgtgt aacagataaa taaacatgat cacttccgtc tttcttcatc
     6181 tgtttgcaga tttctccagt tacaacgtct cttggtttta actcatctgt aaaacgttct
     6241 ccatctttat tcagcaggta agctccctct cctctgactg attcagagat taagaatctt
     6301 cttcctggtt tctttgaata aaatgttgtt ggatggatct ggatatagtt aatattctct
     6361 aattcaacat tatggcgcaa gcagatagcc agactgtctg ctgtcagatg tctgaagttt
     6421 gtagaatgtt taaataaccc tccaagtcct cctgttgcaa gaacagtatc cggagcttcg
     6481 atcgtatcta aattaccatc tgcatcttta tatacaattc ctttacattc tccgtctttc
     6541 tcaatgatat cgatcatcgt acaattttcc atgatcgtaa tattatcacg tttcttcgcc
     6601 tcttcgtata aatggcttgt gatctctttt cctgtcagat cttcatgatg aagaatacgg
     6661 aatgtggaat gtccaccttc tcttgtaaac tcgatctctc catccttcaa gtcaaatctt
     6721 acaccaaaat ctttcagatc ctgaataatc tgtggtgatt ctttgatcat cttctcgacg
     6781 gcacctttat cattctcgta atgtccggct ttcatagtgt cctcgaaata tgaatcataa
     6841 tcttccggat tcttcagcgc tgagattcct ccctgcgcca aaaatgaatc actttcttca
     6901 acttccttct ttgtaataac gattactttg taatccttcg gaagaaataa tgcattaaat
     6961 agccctgaag caccacttcc tacaatgatc acatctgctg ttttcataat tctctttgcc
     7021 tttccttgga actttcctgt cttactgcgc gattcttaac atctcatcta atgctttctt
     7081 agcatttaat cgcagttctt ctggtaattc tacctgattc ttcatgtctc ttaatgtgtc
     7141 tcttacattc tctaaactga tacgcttcat atttggacag aactgacgat gtcctacaga
     7201 gtagaatgtc tttcctgggt tcttctgttt taattcatat aaaacaccca tctctgtaca
     7261 aatgataaat acgtccttgt cagatgctgt cgcatagtca atgattcctg atgtacttcc
     7321 tacataatca cctaaagcta caacgtctgg tgtacattct ggatgtacta aaacttctgc
     7381 ttctggatgt acttctttgg ctttctttac gttctcggct gtgatacttc tatgtacatg
     7441 acagaatccg tcattgtaaa taaagttctt ctctggaagt ttgcttccga tataacgtcc
     7501 gaggttctca tctggtacga agaaaatatt cttctgtgga agtgctgtta caactttcag
     7561 agcattggat gaagttacac agacatcaga atgtgctttg atctctgctg tagagttaat
     7621 ataacagaca acagcaagat catcatattt cttacgcatc tcttcaatct tctctacatc
     7681 gaccatatga gccattgggc agtctgcctt ctgatctggc atgatcactg tacggtctgg
     7741 gtttaagatc ttggcactct cacccataaa gtttacgcct gcaaaacaga tcacatcttt
     7801 ggattctttt aatgcaactt tggcaagata ataagaatcc ccaacataat cagcaatttc
     7861 ctgaacggca tcgtctacat aatagtgtgc tagtaaaaca gcattttttt cttgttttaa
     7921 ttgttcgatc tcttttttta aattttccat ctttctctcc ttgttattaa attttaatat
     7981 accacgacca ccccttctgt tcaagtattt gtgatgtttt tatatgataa ataccagtga
     8041 ttttacctct ttttcagatg cttcaatcac tggtattatt ataaattcta tttattttat
     8101 atggtcacta tatattttca tatgcatttg ctgttttcgt ccgatcatat cttgtacatt
     8161 gtctcgtaat tattttttca catagcatgt gttctttcct ctgcctctct gcatgatcaa
     8221 tcctttttta accatctgct tcaaaatcct tccactcgtt gtctgactga ttcctaatct
     8281 ttcctgaact tgttttcttt gaattctttt ttcttgatcg atcatttgca tgatctgtgt
     8341 ttcttcgtct tctggttgta tatgtgtatg gtattgcagt tcttctctta attctgcctg
     8401 tgcatttgtg tttggtaata tgatcttaaa tacattatta ctgactttga tctctggttt
     8461 tataaactga tcttgataag cattcatgat cttttgtatt cctgttccat aagcttcgat
     8521 caattctaaa cggtaaaaaa cattcgctaa tcttttattc ctgcatacag aaatccccat
     8581 catcacatct tctaatgtaa ctcctgttac caatccccca atggatgtga attctaaccg
     8641 atcttcgtac atactgatca gaatactaga actatatccg taatctctgt gtacaagaca
     8701 gtttagcaat gcttctctta ctgctacttc tggataatct ctattatcaa ttcgataaag
     8761 tttttcaaaa gttgaatgtg tttgatttct gaagtcaatg taatcatata ctgcttccat
     8821 ttgtgcaaat agtgatcctg taaattcttt tctatcttta aatatatttt gattctttcc
     8881 ttcaaaaact gctgttttaa tcgtatgcac gcattggtct gaaagtaata aagcaagatt
     8941 cgtataaatt ccatcttggt ttataattcc taaagttttc atctgtggct gttctaatgt
     9001 gatgtttctc ttttggaatt ctttttttgt ttcgcaaaaa gtcaattctt gatctaatga
     9061 tctcatatct tcaaaatgat caccatctgt ttctttaatc atctttctaa tagctgtatc
     9121 cgttgctggg acagaagaat atccttgtct tacatatacg ccttctggtc ttaacccttt
     9181 cttcgctata tagtatgggc gttctgttcc tctttgaata tctatgatca caacttgttt
     9241 ttgttctatc tcttttgtct cataatgcaa gaacatggtt aaatctggtt taatcgcatc
     9301 tctaaccata ttgcttactt gcaatgctac actgtctgaa tcttctaccc cgataactgt
     9361 cccatcatct tcaattccaa tatagatttt tcctccatca caattggcaa atgctatgat
     9421 ttctttcttg atgtctgtga ctacaatgga tttgaattcc gttctctcag attcttgtaa
     9481 cttcatgaaa aaccctcctt gaaattattc tgtctgttct ttctaacttt attctaacca
     9541 ttctaaccat tcttgtcaat tataaatcac agtgtttgta tgctgtatag tggtttgaat
     9601 cacgtaaatt atttgaatat tataaaaaga gatcatg
//
LOCUS       DAOMUE010000042         9393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1825_length_9393_cov_2.181838, whole genome shotgun sequence.
ACCESSION   DAOMUE010000042 DAOMUE010000000
VERSION     DAOMUE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9393
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1825_length_9393_cov_2.181838"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PLC71_03385"
     CDS             complement(<1..877)
                     /locus_tag="PLC71_03385"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRM01721.1"
                     /translation="MKHFNEQISKAKTIAITGHIHPDGDCIGSCLALKQYILDNYSGK
                     KVDVYLESISTEFRFLSHAQEIITESKDDESYDLFFVLDCGSEDRYEPFAAMVRCAKT
                     LIGIDHHISNDGFGDFYKIDPQASATCEVLCQIFEEDKISKECAQCLYTGIVHDTGVF
                     KHSNTTRKTMEYAGMLLEKGVSTTKIIDETFYQKTFVQNQLLGKALLKSQLYADGQII
                     ISNLSEQDFEELHASTSDSDGIIDQLRITKGVEAAIFLYPIENGYKVSMRSNEKVKVS
                     EIAKTHGGGGHIRAAG"
     gene            complement(864..1235)
                     /gene="rbfA"
                     /locus_tag="PLC71_03390"
     CDS             complement(864..1235)
                     /gene="rbfA"
                     /locus_tag="PLC71_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRM01722.1"
                     /translation="MRKNSIKNTRINGEVQRELSRIISREIKDPRIAPMTSVVDAVVT
                     SDLKQCKAYISVLGNDEEKEETMKGLNSAVGYIRRELAHSINLRNTPEITFILDDSIE
                     YGVNMSKKIDELNHGSENETL"
     gene            complement(1257..3509)
                     /gene="infB"
                     /locus_tag="PLC71_03395"
     CDS             complement(1257..3509)
                     /gene="infB"
                     /locus_tag="PLC71_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRM01723.1"
                     /translation="MAKFRVYEIAKKYNKDNKEILEILKANHVEVKNHMSTIGDEQIK
                     MIENALKPKKEAKQDHNSKKQENSKRKDNKKAQNMEKKNNKKTQEVKNNKNNSRNKNN
                     DGDKKILRKDNPQNSQKFGKNNKKNKKFNNKNQNQNQEHKKKKKTSGPGAFIKPEPVK
                     KPEVDPNAPVKIPPVLTLKELADALSIPANDIIKKLLISGAGMLNVNSELDFEKAEEI
                     AMEFDRLVEMEEQVDVIEELLKEEEEDETDMIVRPPVVCVMGHVDHGKTSLLDKIRSS
                     HVTTGEAGGITQHIGAYVVETSNGKITFLDTPGHEAFTSMRMRGAQSTDIAILVVAAD
                     DGVMPQTIEAINHAKAAGIEIIVAINKIDKESANIERVKQELIEYELVPEDWGGSTIF
                     CPVSAHTGEGIDELLDMVSLTAEVLELKANPNRKARGIVLEAQLDKGRGPVATVLVQK
                     GTLHVGDAVAIGSAHGKVRAMINDKGKRIKTAGPSTPVEILGLSEVPNAGEVMMVMDS
                     EKEARSVAEKFIAYGREKMLSETKQQLSLDDLFNQIQAGSVKELNIIVKADVQGSVEA
                     VKQSLVKLSNDEVAVKVIHGGVGAITESDVNLAAASNAIIIGFNVRPEPAAKDAASAE
                     KVDLRLYRVIYNAIEDIEAAMKGMLDPEFVEKVTGHAEVRQIFKASGVGTIAGSYVLD
                     GTIQRDSSARIIRDGIVVHEGKLASIQRGKDAVKEVRSGFECGLVMESYNDIKEGDQI
                     ESFIMEEVPR"
     gene            complement(3530..3829)
                     /locus_tag="PLC71_03400"
     CDS             complement(3530..3829)
                     /locus_tag="PLC71_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HRM01724.1"
                     /translation="MNKGLSLLGLAFRSGNLVSGEFAAREAVRKKTAALIIVANDASD
                     NTKKMFENQCKHYQVPFYCWGLKEELGHAIGKEFRASIAVTDQGFAEALLKQLDK"
     gene            complement(3822..4091)
                     /locus_tag="PLC71_03405"
     CDS             complement(3822..4091)
                     /locus_tag="PLC71_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HRM01725.1"
                     /translation="MNRKIPTRKCIGCGELKEKNQLVRIVRSKEGEISLDMTGKKNGR
                     GAYICPDPQCLKQAFKKKGLDRSFKIGVPESVYETLKKEMEELYE"
     gene            complement(4113..>4407)
                     /locus_tag="PLC71_03410"
     CDS             complement(4113..>4407)
                     /locus_tag="PLC71_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HRM01726.1"
                     /translation="VEEKSAEVVVPDYQLSLAIGNEGQNARLAARLTGYKIDIKSESQ
                     AAEEAAKVSEEPEADEFEIEEDLFNEEINDDFVEDAENTEEAVEDFDAEDIEE"
     assembly_gap    4408..4417
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4418..5309)
                     /gene="nusA"
                     /locus_tag="PLC71_03415"
     CDS             complement(<4418..5309)
                     /gene="nusA"
                     /locus_tag="PLC71_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRM01727.1"
                     /translation="MSELIAALNQLEKEKGIDKDVIMEAIENSLLAACKRDFGSADNV
                     VVNMDRETGDIYVYAIKEVVDEVYDPALEISLGKAKAIDQNLNLGDTVRIEITPKDFG
                     RIAAMHARSVIVQKIKEEERRVVYDHFYCKEKDIVTGVVQRYVGENVSVSLDDKTDAL
                     LMKSEQIRGEVFKPTERIKLYIVEVKETNRGPRILVSRTHPDLVKRLFEKEVAEIQDG
                     TVEIKNIVREAGSRTKMAVWSNDKNVDPVGACVGLNGARVNAIVNDLKGEKIDIINWN
                     EDPCVFIENALSPSKVVSVAV"
     gene            complement(5325..5789)
                     /gene="rimP"
                     /locus_tag="PLC71_03420"
     CDS             complement(5325..5789)
                     /gene="rimP"
                     /locus_tag="PLC71_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRM01728.1"
                     /translation="MARRVDIETKTEELVLPIIEANNFELVDVEYVKEGANWYLRVYA
                     DKEGGIAIDDCVLISRSLEEKLDAEDFIEDAYILEVSSPGLGRPLKKEKDYVRSVGKS
                     IDIKLYKAIDKQKEFTGILKENMEDQIVLTIEDQDITFEKKSIASARLSLDF"
     gene            complement(5937..>8762)
                     /locus_tag="PLC71_03425"
     CDS             complement(5937..>8762)
                     /locus_tag="PLC71_03425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HRM01729.1"
                     /translation="AGFDTGFIRENAKRLNLPYDHTVVDTLGLARCLMGHLGKFTLDN
                     ICKHLNIILETHHRAVDDATATGKIFVEFISMLKEKDITDLDQVNEFANDNVDLIKKG
                     RMYHGIILVKNNTGRVNLNRLVSESHLNYFNRRPRMPKSLINKYRDGLIIGSACEAGE
                     LYQALLDERSDETIANIVSFYDYLEIQPVGNNEFMIGSERVENVNSIEDIQKFNQKIV
                     DLGEQFHKPVVATCDVHFLNPDDAIYRSILLAGKGFKDADQQAPLYFRTTQEMLDEFA
                     YLGSEKAKEVVITNTNKINDMIENISPIHPDKCPPVIPDSDKTLREICYNRAHEIYGD
                     DLPERVTSRLEKELNSIIGNGYAVMYIIAQKLVWDSNDHGYLVGSRGSVGSSFAATMS
                     GITEVNPLPAHYICPECHFVDFDSEQVQKYAKMGMSGFDMPDAYCPKCGAKMTKEGQD
                     IPFETFLGFKGNKEPDIDLNFSGEYQGKAHAYVEVIFGKGKAFRAGTIGTLAEKTAYG
                     YVLKYLEERGISKRRCEIERLALGCTGVKRTTGQHPGGIIVVPHDKEIYDFTAVQHPA
                     NDMNTPIITTHFEYHSIDQNLLKLDILGHDDPSMIRRMEDITGIDAQTIPMDDKGVMG
                     LFHGTETLGITPEDIDGTPLGSLGVPEFGTDFVIQMLQDTHPQSFSDLVRISGLSHGT
                     DVWLGNAQTLIENGDAVISTAICCRDDIMVYLINQGLEQEASFKIMEGVRKGKGLTDE
                     QEQMMRDHNVPDWYIWSCKQIKYMFPKAHAAAYVMMAWRIAWYKVYHPLAYYAAYFSI
                     RAKAFSYEDMCLGKERLDEKMSIIKNTPKHEVKNADLDLLREMKIADEMYARGYEFWP
                     LDIYKAKAKDFQVIDGKIMPALTSIDGMGEKAAQQVEEAAKGEPFTSLENFRNRTKAP
                     QACIEKMKELGIMGDLSDTDQLSFDFL"
     assembly_gap    8763..8772
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8773..>9393)
                     /locus_tag="PLC71_03430"
     CDS             complement(<8773..>9393)
                     /locus_tag="PLC71_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HRM01730.1"
                     /translation="MYDKFDHEISLGSVAGIKAITDFREKRQDTSMEKRVELHLHTVM
                     SDNDSVVQIKDIVNRAYEWGHPAVAITDHGVLQGFPIARHAYEDLRLKEDDPFKIIYG
                     VEGYFVDDLGDLIIDSKGQSLMDSYVVFDIETTGFNSVNDRIIEIGAVRVVEGEIKET
                     FSEFVNPERPIPYKITQLTTITDDMVKDAGTIEEILPQFLKFCEGSV"
BASE COUNT         2428 a         2163 c         1391 g         3391 t
ORIGIN      
        1 atcctgctgc acggatatgt cctccacctc catgtgtttt agcaatctct gaaaccttaa
       61 ctttctcatt cgaacgcata cttactttat agccattttc gatcggatat agaaaaattg
      121 ctgcttccac accttttgta attcgcagct gatcaatgat cccatcactg tcagatgttg
      181 atgcatgaag ctcttcaaaa tcttgttctg aaagattcga aataataatc tgtccatctg
      241 cataaagttg actttttaat aatgctttcc ctaacaactg attttgtaca aatgtcttct
      301 gataaaatgt ctcatcaatg atctttgtag ttgaaacacc cttctccagt aacattcccg
      361 catattccat cgttttacgt gttgtatttg aatgcttaaa cactccggta tcgtgcacga
      421 ttcctgtgta taggcactgg gcacattctt ttgaaatctt atcctcttca aaaatctggc
      481 acaaaacttc acaagttgca ctcgcctgtg gatcgatctt ataaaagtcc ccaaatccat
      541 cattactgat atgatgatcg attccgatca atgtttttgc acatctgacc attgctgcaa
      601 atggttcata acgatcttca gagccacaat ctaaaacaaa aaacagatca tagctttcat
      661 catctttcga ctctgtgatg atctcctgcg catgacttaa gaaacgaaac tctgtgctga
      721 ttgattctaa ataaacatca actttcttcc cagaataatt atctaaaata tactgtttta
      781 aggcaaggca actgccaata cagtctccat ccggatggat gtgtcctgta atggcaattg
      841 ttttagcctt tgaaatctgt tcattaaagt gtttcattct cactcccatg atttaattca
      901 tctatcttct ttgacatatt tactccatat tcaatggaat catctaaaat aaatgtaatc
      961 tctggtgtat ttcttaaatt aatagagtgt gcaagttctc ggcggatata acctaccgca
     1021 ctgtttaagc ctttcatcgt ttcttctttt tcttcatcat ttccaagaac actgatatat
     1081 gctttgcact gtttcagatc cgatgtcaca acggcatcaa caacagatgt cattggtgca
     1141 attcttggat ctttgatctc tctgctgatg atacggctta attctctctg aacttcacca
     1201 ttgatccttg tatttttaat gctgtttttt ctcatattgc tactcccttc aggaaattat
     1261 cttgggacct cttccataat aaaggattcg atctgatccc cttctttgat gtcattatag
     1321 ctttccataa caagaccaca ttcaaatccg ctgcgaactt ctttgaccgc atcttttcca
     1381 cgttggatag atgcaagttt tccttcatga acaacaatac catcacgaat aatacgtgct
     1441 gagctgtcac gctgaattgt tccatctaat acataagatc ctgcgatcgt tccaactcca
     1501 gaggctttga aaatctgacg tacttctgca tgccctgtaa ctttctctac aaattcagga
     1561 tctagcatac ccttcatagc tgcttcaata tcttcaatcg cattgtagat gacacgatac
     1621 agacgaaggt ctactttctc tgcacttgct gcatctttag ccgctggttc tggacgaaca
     1681 ttaaatccga taatgattgc attagaagct gctgctaagt ttacatcaga ttctgtgata
     1741 gcacctacac caccatgaat gactttgact gcgacttcat cattagaaag ttttacaaga
     1801 ctctgtttaa ccgcttctac agatccctgt acatcggctt ttacaatgat attcaattct
     1861 ttgacgcttc ctgcttggat ctggttaaat agatcatcta atgataactg ctgttttgtc
     1921 tcagataaca ttttttcacg tccataagca ataaatttct ctgctacgga tcttgcttct
     1981 ttttcgctat ccataaccat cataacttct ccagcatttg gaacttcact taaacctaag
     2041 atctcaactg gtgtagatgg tcctgctgtt ttaatacgtt ttcctttgtc atttatcatt
     2101 gcacgaactt taccatgagc acttccgatc gctactgcat caccaacatg taatgtacct
     2161 ttctgtacta atacagttgc aactggtcca cgacctttat caagttgtgc ttctaagaca
     2221 atacctcttg ctttacgatt tggatttgct tttaattcca aaacttctgc tgttaaagaa
     2281 accatatcta ataattcatc gattccttct cctgtatgtg cagatactgg acaaaagatc
     2341 gtgcttccac cccagtcttc tggtaccaat tcatattcga tcagttcctg ttttactctc
     2401 tcaatgttgg cgctttcttt atcaatctta ttgatcgcaa caatgatctc gattcctgct
     2461 gctttcgcat ggttgatcgc ttcgatcgtc tgtggcataa caccgtcatc tgcagctacg
     2521 actaagattg cgatatctgt agactgggca cctctcatac gcatagatgt gaatgcctca
     2581 tgtcctggtg tatctaagaa tgtgatcttt ccattggatg tttcaacaac ataagctcca
     2641 atatgctgtg tgataccacc tgcttctcct gttgtaacat gagaagaacg gatcttgtca
     2701 agtaaagaag ttttaccatg gtcaacatga cccataacac agacaactgg tggtcttacg
     2761 atcatatctg tttcgtcttc ttcctcttct tttaacagtt cttcgatcac atctacctgt
     2821 tcttccatct ctaccaggcg atcaaattcc attgcgatct cttctgcttt ttcaaaatca
     2881 agttcagagt ttacatttaa cattccagct ccgctgatca gtaatttctt aatgatatca
     2941 tttgcaggaa tagataatgc atctgcaagt tctttcaatg ttaatactgg aggaatcttt
     3001 actggcgcat ttggatctac ttccggtttc tttactggtt ctggtttaat aaaggcacca
     3061 ggtcctgatg tttttttctt tttcttatgc tcctgattct ggttctggtt tttattgtta
     3121 aatttcttat tctttttatt atttttccca aacttctgag agttctgagg attatctttt
     3181 cttaagatct ttttatcccc atcattattt ttatttcttg aattattttt attgttcttt
     3241 acttcctgag tttttttatt atttttcttt tccatattct gtgctttttt attatctttt
     3301 ctttttgaat tttcctgttt ctttgaatta tgatcctgtt ttgcttcttt ctttggtttc
     3361 aacgcattct cgatcatttt gatctgctca tctcctatgg tactcatgtg gtttttcact
     3421 tctacatgat tggctttcaa gatttctaaa atttctttat tgtctttatt atatttcttt
     3481 gcaatttcat aaactctgaa ttttgccata aatatacctc ctgcgattct tatttatcaa
     3541 gctgttttaa aagagcttct gcaaaacctt gatcagtcac tgcgatcgat gctcgaaact
     3601 ccttcccgat cgcatgccca agctcttctt ttaatcccca gcaataaaaa ggtacttgat
     3661 aatgtttaca ttgattctca aacatctttt ttgtattatc ggaagcatca ttggctacaa
     3721 tgataagagc tgctgtcttt ttcctgacag cttctcttgc ggcaaattct ccactgacca
     3781 gattaccgga tctgaatgca agacctagaa gagataatcc tttattcata aagttcttcc
     3841 atctcctttt ttaatgtctc atagaccgat tccggtactc ctatcttaaa agaacgatct
     3901 agtccttttt tcttaaatgc ttgttttaga cactgtggat cagggcatat atatgcccct
     3961 ctcccatttt tctttccagt catatcaaga ctgatctcgc cttcttttga tcttacgatc
     4021 cggactaatt gattcttttc ttttaattca ccgcacccga tacattttct tgttggaatt
     4081 tttcgattca ttttatcacc tgctgatatt tcctattctt caatatcctc tgcatcaaaa
     4141 tcttcaacag cttcttctgt attctctgca tcttctacga aatcatcatt gatctcttcg
     4201 ttaaaaagat cttcttcaat ctcaaattca tctgcctctg gttcttcact gacttttgct
     4261 gcttcttcag ctgcctgtga ttcactctta atgtcaatct tatagcctgt taatcttgca
     4321 gctagtctcg cattctgtcc ttcgttaccg attgctaatg ataactggta atctggaact
     4381 acaacttctg cactcttttc ttctacannn nnnnnnncaa ctgccacaga aacaactttt
     4441 gatgggctta atgcattctc aatgaataca caaggatctt cattccagtt gatgatatcg
     4501 attttctcgc cttttaagtc attgacgatt gcattcactc tggcaccatt taatccgaca
     4561 catgctccaa ctggatctac attcttatca ttggaccata cagccatctt tgttcttgat
     4621 cctgcttcac gcacaatgtt cttgatctct actgttccat cctgaatttc cgcaacttct
     4681 ttctcaaata aacgttttac aagatctgga tgtgttcttg aaacaagaat acgtggtcca
     4741 cgatttgttt cttttacttc tacaatataa agtttgattc tctctgttgg tttgaacact
     4801 tcacctctga tctgttcaga tttcattaac agagcatctg ttttgtcatc taagcttaca
     4861 cttacattct ctccaacata acgctgaaca acacctgtta cgatatcttt ttctttgcag
     4921 tagaaatgat catatacaac tcttctttct tcttctttga ttttctgcac aataacgctt
     4981 cttgcatgca ttgctgcaat acgtccgaaa tcttttggtg tgatttcaat tcttacagta
     5041 tctccaagat ttaagttctg atcgattgcc tttgcttttc caagactgat ctccagagca
     5101 ggatcataaa cttcgtctac aacttctttg atcgcataaa cataaatatc tcctgtttca
     5161 cgatccatat taactacaac gttatctgca cttccaaaat ctcttttaca tgcagcaagc
     5221 agggaatttt caatggcttc catgataacg tctttatcaa tacctttttc cttctccaac
     5281 tgatttaatg ccgcaattaa ttcactcatt ctctttcttc ctccttaaaa atctaacgac
     5341 aaacgagcac ttgcaatgct ctttttctca aatgtaatat cctgatcttc gattgttagt
     5401 acaatctgat cttccatgtt ttcttttaaa ataccagtaa attccttttg cttatcaatt
     5461 gctttataaa gcttgatgtc aatggacttt ccgacacttc ttacataatc tttttctttt
     5521 tttagtggtc ttccaagtcc tggagaactt acttctaaaa tatatgcatc ttcgataaaa
     5581 tcttctgcat ctagtttctc ttctaagctt ctgctgatca gcacgcagtc atcaattgca
     5641 attccacctt ctttgtctgc gtaaactctt aaataccagt ttgctccttc tttgacatat
     5701 tctacatcca ccagttcaaa attgttggct tcaatgatcg gtaataccag ttcttctgtt
     5761 tttgtttcaa tatcaactcg tcttgccaat gtatcaaccc tttcttttgt agtgttattt
     5821 cttcattgtt acacaaaata ggagtggaca tatgcccact cctaagtcat cttttcttaa
     5881 gctatattta gtttaacatg aaaaatatct cttgacaaga gttttttcat atattatcaa
     5941 agaaaatcga aacttaactg atcagtatct gatagatctc ccataattcc aagttctttc
     6001 attttctcga tacatgcttg tggtgctttt gttctgtttc taaagttttc caaggatgta
     6061 aatggttctc ctttggctgc ttcttctacc tgctgtgccg ctttttcacc cattccatca
     6121 atactggtaa gtgctggcat gatctttcca tcgatcactt gaaaatcttt tgcctttgcc
     6181 ttgtaaatat ccagtggcca gaattcatag cctcttgcat acatttcatc cgcaatcttc
     6241 atttctcgta acagatcaag atctgcattt ttaacttcgt gttttggtgt attcttaata
     6301 atactcatct tttcatccaa acgttctttt ccaagacaca tatcttcata agaaaatgcc
     6361 ttcgctcgaa tactgaaata tgctgcataa tatgctaatg ggtgataaac cttataccat
     6421 gcgatcctcc atgccatcat aacataagct gccgcatggg ctttcgggaa catatatttg
     6481 atctgtttac aagaccagat ataccaatct ggcacattgt gatctctcat catctgttct
     6541 tgttcatctg taagtccctt tccttttcgg actccttcca tgatcttaaa tgacgcttcc
     6601 tgttccaatc cctgattgat cagatatacc atgatatcat ctcgacaaca gattgctgtg
     6661 gaaataaccg catctccatt ctcgatcaat gtctgagcat ttccaagcca tacatcggtt
     6721 ccatgagaca gaccagaaat acggacaaga tcagaaaaac tctgtggatg cgtatcctga
     6781 agcatttgaa ttacaaagtc tgtcccaaat tccggtactc caagtgatcc aagcggtgtc
     6841 ccatcaatat cttctggtgt gatccctaag gtttctgttc catgaaataa tcccataacg
     6901 cctttatcgt ccattgggat cgtctgtgca tcaattcctg tgatatcttc cattcttcgg
     6961 atcatagatg gatcgtcatg tcccagaata tcaagtttta ataggttctg gtcaatcgaa
     7021 tggtattcaa aatgtgtcgt aatgatcggt gtattcatat cattggctgg atgttgtacc
     7081 gctgtaaaat cataaatttc tttatcatgc ggtactacaa tgattccacc cggatgctga
     7141 ccagttgttc tcttaactcc cgtacatcca agtgctaatc tctctatttc acaacgacgt
     7201 ttactgattc ctcgttcttc cagatatttc aacacataac cataggctgt tttctctgcc
     7261 agtgtaccga tcgttcccgc tcggaatgct tttccttttc caaagataac ttctacataa
     7321 gcatgtgctt ttccctgata ttctcctgag aagtttaagt cgatatccgg ctctttattt
     7381 cctttaaacc caaggaatgt ctcaaacggg atatcctgtc cttctttcgt catctttgct
     7441 ccacactttg gacagtatgc atctggcatg tcaaaacctg acatacccat cttcgcatat
     7501 ttttgcacct gttctgagtc aaaatccaca aaatgacatt ctggacagat ataatgtgct
     7561 ggcaacggat ttacttccgt gatccctgac atcgttgctg caaaggaaga tcctaccgat
     7621 cctcgtgatc ctaccagata cccatggtca ttcgaatccc ataccaattt ctgtgcgatg
     7681 atgtacatta ccgcatatcc atttccaata atagagttta attctttttc caaacgactt
     7741 gtcactcgtt ctggaagatc atctccataa atctcatgtg ctctattata acagatttct
     7801 cttaatgttt tgtctgaatc tgggattacc ggcgggcatt tatccggatg gatcggagaa
     7861 atattttcaa tcatatcatt gatcttattc gtgtttgtta tgacaacttc tttggctttc
     7921 tcacttccaa gatacgcaaa ctcatctagc atctcctgtg ttgtcctaaa ataaagtggt
     7981 gcctgctggt cagcatcttt aaatcctttt ccagctaata aaatagaacg atagattgca
     8041 tcatctggat ttagaaaatg tacatcacat gtcgcaacga ctggcttatg gaattgttct
     8101 ccaagatcta cgatcttttg attgaatttc tgaatatctt cgatcgaatt cacattctca
     8161 acacgttcgg atccaatcat aaattcattg tttcctactg gctgaatctc cagatagtca
     8221 taaaatgata caatattcgc aatcgtttca tctgagcgtt catcaagcaa tgcctgatat
     8281 aattctcctg cttcacacgc agatccaatg atcaatccat ctctatattt attgatcaga
     8341 gactttggca ttcttgggcg acggttaaag taatttaaat gtgattcgga taccaagcgg
     8401 tttaaattaa cacgccctgt attattctta accagaataa ttccatgata catgcgtcct
     8461 tttttgatca gatcgacatt atcatttgca aattcgttga cctgatctag atctgtaata
     8521 tctttttctt ttaacataga aatgaattct acaaaaatct ttcctgttgc tgttgcatca
     8581 tcaactgctc tatggtgtgt ttccagtatg atatttaaat gtttacaaat gttatccagt
     8641 gtaaatttac caagatgtcc cattagacat cttgcaagcc ccagtgtatc cacaactgta
     8701 tgatcatatg gaagattcaa ccttttcgca ttttcgcgga taaaccctgt atcaaatcct
     8761 gcnnnnnnnn nnaacagatc cctcacagaa ttttaaaaac tgtggaagaa tctcttcgat
     8821 cgttcccgca tcttttacca tatcatctgt gatcgttgtc agctgagtga tcttatatgg
     8881 aattgggcgc tctggattga caaattcact gaatgtttct ttaatctctc cttccacgac
     8941 tcttaccgca ccaatttcta taattcggtc attgacactg ttaaatcctg ttgtctcaat
     9001 atcgaataca acataactgt ccattagtga ctgccctttt gaatcaatga tcaaatctcc
     9061 aaggtcatca acgaaatatc cttctacccc ataaatgatc ttaaatggat catcttcttt
     9121 tagtctcaag tcttcgtatg catgacgtgc gattggaaat ccctgcaata caccgtgatc
     9181 tgtaatcgca accgccggat gtccccattc gtaggcgcga tttacaatat ctttgatctg
     9241 taccacactg tcattatcgc tcatcactgt atggagatgt aattccacac gtttttccat
     9301 ggatgtgtcc tgacgttttt ccctgaaatc tgtaatcgcc ttgattcctg ccacagatcc
     9361 tagagagatc tcatgatcaa atttgtcata cat
//
LOCUS       DAOMUE010000043         9244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1880_length_9244_cov_3.446403, whole genome shotgun sequence.
ACCESSION   DAOMUE010000043 DAOMUE010000000
VERSION     DAOMUE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1880_length_9244_cov_3.446403"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="PLC71_03435"
     CDS             complement(<1..175)
                     /locus_tag="PLC71_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HRM01731.1"
                     /translation="MLPYISEFIGTMLLILLGDGVVANVNLERSGMKGAGAVQITFAW
                     GLAVLVPAFIFGSS"
     gene            complement(215..1708)
                     /gene="glpK"
                     /locus_tag="PLC71_03440"
     CDS             complement(215..1708)
                     /gene="glpK"
                     /locus_tag="PLC71_03440"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HRM01732.1"
                     /translation="MKKYIMALDAGTTSNRCILFNEQGVICSVAQKEFTQYFPHPGWV
                     EHDADEIWSSMLGVAVEAMNKLNITPDQVAAIGITNQRETTIIWDKNTGEPVYHAIVW
                     QCRRTSEYCDQLKEKGLTEKFRQKTGLMIDAYFSGTKVKWILDNIPGAREKAEAGDLL
                     FGTVETWLIWKLTKGKVHVTDYSNAARTMLFNINTLEWDDEILEELGIPKSMLPQARP
                     SSEVYGMADESYFGKEIPIGGAAGDQQAALFGQTCFTAGEAKNTYGTGAFLLMNTGTK
                     PVFSDNGLITTIAWGLNGEVNYALEGSIFVAGAAIQWLRDEMRLVDSSPDSEYMAKKV
                     KDTNGCYVVPAFTGLGAPHWDQYARGTIVGITRGVNKYHVIRATLESLAYQTYDVLKA
                     MEADSGIKLSALKVDGGASANNFLMQFQSDILNTEVRRPRCVETTAMGAAYLAGLAVG
                     YWKDKNDVINNWNIDREFHPEMQEAEREEKLAGWEKAVKYSFGWAKN"
     gene            complement(1890..2933)
                     /locus_tag="PLC71_03445"
     CDS             complement(1890..2933)
                     /locus_tag="PLC71_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HRM01733.1"
                     /translation="MSVITFVCAGQMNSAITFPAFENGHEVRLVGSPLDRDIIDGLKK
                     DNFHITLKRTLHDGIKYYQIEELEEALEGADLILGGVSSFGLDWFCDEILPVLPEDVP
                     LLTVTKGMVDLDDGTLVPYPHIFEQRQPEGKHINFNAIGGPCTSYELADHDDSHVAFC
                     GKDMETLKFIKSLLTTDYYHISLSTDVVGVECAVAMKNAYALGVSLAVGLAEKRDGEI
                     GAVHYNSQAALFGQGVKEMIHLLELIHGGPENIIHGAGDLYVTVFGGRTRKIGTLLGR
                     GLTFDQAMEELQGVTLESIVIATRTARAVRKLAERGVVSLDDFPLLMHVDAIINEGAE
                     VDIPWEKFTTVVE"
     gene            complement(2978..3328)
                     /locus_tag="PLC71_03450"
     CDS             complement(2978..3328)
                     /locus_tag="PLC71_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HRM01734.1"
                     /translation="MTDLICIVCPKGCHLKVDEENGYKVTGNGCPRGAAYGEKELVNP
                     TRVITSTVRVEAKTARRVPVKTASDIPKGKIYDIMKELDKVTMKAPVAIGDVVIPNVL
                     GLGVDVVACKKVAE"
     gene            complement(3331..4596)
                     /locus_tag="PLC71_03455"
     CDS             complement(3331..4596)
                     /locus_tag="PLC71_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM01735.1"
                     /translation="MKYDVIVVGGGPAGLAAAVEASKNGAEKVLVVERDQELGGILNQ
                     CIHNGFGLHYFKEELTGPEYAGKFIDMLNETNIEVMLDTMVLDVDETEKCVHAMNKKN
                     GYMKLEGKSIILNMGCRERTRGAIAIPGDRPAGVFTAGAAQRYVNIEGYMVGKKVLIL
                     GSGDIGLIMARRMSLEGAKVVGCVELCPYSNGLNRNIVQCLNDYDIPLYLSHTITDIQ
                     GDKRVEKVIVSKVDEKNQPIPGTEMEFDVDTVLLSVGLIPENELTRSAGITMDPATSG
                     PVVYENMETSAEGIFASGNVVHVHDLVDFVTAESQKAGKSAAEYVKNGETKDQTCIDI
                     KNGDGVNGIVPQKVRPSNVDKKVEVMFRARNVFTDVAIKVRSNGEELASFKREHMAPG
                     EMEKIVIPKAFLDKVENGEITVSVEKVGA"
     gene            complement(4596..6035)
                     /locus_tag="PLC71_03460"
     CDS             complement(4596..6035)
                     /locus_tag="PLC71_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM01736.1"
                     /translation="MYDVAIIGAGITGCAIAYELGKYNVKAVIVEKENDVSVGTTKAN
                     SAIIHGGYDPKPGTKMAEYNIKGNVYTKELCEKLDVPYKQIGALVVAFSEEEKKTLEE
                     LKQRGITNGVPDMEIWDKEKLLKEEPNLSDKAVAALSSPNVGIVSPWELALALAETAV
                     LNGVEVKLNTEVSGIEKENDTYKIETNNGVIEAKYICNAAGVFADKVNEMCNEKTFEI
                     TPNKGEYYLMDKSQGNLVNHVIFQCPNEKGKGVLVAPTVHGNLIVGPDSQPSAANDVS
                     TTKQGLDFVRNTALKSVPGINFRESIRNFAGVRARTADHDFHIYEDKNNKGFINIAGM
                     QSPGLSSACAIAPDILKMLENSGLTLEAKEDIVDERHIDRFKHLSHEDREKLVEKNPA
                     FGKIICRCETITEGEIINAVHRPIPATSIDAVKRRCNAGMGRCQGGFCGPRVQEIIAR
                     ELNKPLADIPQDRLGTNIITGETKDGGAK"
     gene            6360..7472
                     /locus_tag="PLC71_03465"
     CDS             6360..7472
                     /locus_tag="PLC71_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM01737.1"
                     /translation="MGKRNKVTTRDIAEYTGVSQSTVSMILSNKPHVSFSKETIDKVR
                     QGAKELGYKKPVAGVKKKEQALAKSIIVICPLLSNGYYSMIIHSITERAKDYGYTVFT
                     VSTLRDVSQEELYLNLLSGFELAGVISLYPPTKIAQANALSKQVPVVSIGDKPDACRF
                     DAVELDSKKPGYIIAEHLLSLGHKHITYICTPIRPKEIGRIHRLAGLRSCFKDHGLDP
                     EWVEVKSPTIAAYGRYSADNSEYQNGYDMASQALDEHTSSTAFVGNNDMTAFGIMAAI
                     SDHGFRVPHDYSVCGFDNIPLSSMPQIALTTIEHASLAKGREAVDIIYKKNTQKNISA
                     KHHYIMRMEYEPELIVRNSTGKCKITNKTKECHNIS"
     gene            7567..8028
                     /locus_tag="PLC71_03470"
     CDS             7567..8028
                     /locus_tag="PLC71_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM01738.1"
                     /translation="MASKLLSNEFQKHICLDYECTTIEEAIHKAGDLLVQVGCVKPPY
                     INGMIRREQETSTDIGLGVMIPHGTEDTLNFVLKPGISIVQIPHGVSYHGNTIHLVIG
                     ISSREEDSLQELMQITDILLKPEKCHQFLNASSKQEFIDMINQNYVEEGEL"
     gene            8031..>9244
                     /locus_tag="PLC71_03475"
     CDS             8031..>9244
                     /locus_tag="PLC71_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM01739.1"
                     /translation="MKEKTSISQKLQKFGSVLAGMVIPNIGAFIGFGLITAFFLETGW
                     TPNAKLAKLISPILNYLLPILIGHTGGKMFGGDRGGVIGALVTMGAIVAVDGTPMFLA
                     AMILGPVAGVCIKKFDQAVDGKIPSGFEMIVNNFSLGIIGAILCCFAMLVFGPVMETV
                     TTVLTNGVNWIVAHNALPLSAIFVEPAKVLFLNNALDHGIFGPIGYEQVKTLGHSALF
                     LLVPNPGAGLGLLLAYWFFGKGEMKEAAPGMILIHFFGGIHEVYFPYVLANPLTIIGM
                     IAGGIVGTGTLTFLNVGTVATPSPGSIFSVVALSPKNCMFGVLLSVVLSCVVSFLLNT
                     IFVKRMVAKGGDTSLGESVVPKEAMGSSASSNESTAITNDSSLGVAGVDIKNIKKIVV
                     ACDAGMGSSAMSA"
BASE COUNT         2498 a         1985 c         1532 g         3229 t
ORIGIN      
        1 atgaagatcc aaaaataaat gctggtacaa gcaccgcaag tccccatgca aatgtgatct
       61 gcactgctcc tgcacctttc attcctgatc tttcaaggtt tacatttgct acaactccat
      121 caccaagtaa gattaagagc attgttccga taaattctga aatataaggt aacatagtct
      181 ttccctcttt tctttttaat ttttcatgat atatctaatt tttagcccat ccgaaagaat
      241 attttactgc cttttcccat ccagctaatt tttcttctct ttctgcttcc tgcatttctg
      301 gatggaattc acggtcaata ttccagttat tgataacgtc atttttatct ttccagtatc
      361 caactgcaag tcctgcaaga taagccgctc ccattgcagt tgtctcaacg catcttggac
      421 gacgtacttc tgtgttaaga atatctgact gaaactgcat taagaagtta tttgcacttg
      481 ctccaccatc aacttttaat gcacttaatt tgattccact gtccgcttcc attgctttta
      541 acacatcata tgtctgatat gccagagatt ctaatgtcgc acggatcaca tgatatttat
      601 tgacacctct tgtaattcca acgatcgttc cgcgtgcata ctgatcccaa tgtggcgcac
      661 caagtcctgt aaatgcaggc actacataac agccatttgt gtcttttact ttctttgcca
      721 tatattcaga atctggagat gaatccacaa gtctcatctc atctcttagc cactgaattg
      781 ctgctccagc aacgaaaata gatccctcta atgcatagtt gacttctccg tttaatcccc
      841 atgcaatagt tgtgatcagt ccattatcag aaaagactgg ttttgttcct gtattcatta
      901 acaggaatgc tccagttcca tatgtatttt tagcttcacc tgctgtaaag catgtctgtc
      961 cgaacaatgc agcctgctga tctcctgctg ctcctccaat aggaatctct tttccaaaat
     1021 agctttcatc tgccattcca taaacttcac tggatggtct tgcctgtgga agcattgatt
     1081 ttggaattcc taattcttca agaatttcat catcccattc aagggtatta atattaaata
     1141 acattgttct tgccgcattt gaataatctg taacatgaac ttttcctttt gtaagtttcc
     1201 agatcagcca tgtctctacc gttccaaata aaagatctcc tgcttcagct ttttcacgtg
     1261 cccctggtat attgtcaaga atccatttta cctttgttcc actaaaataa gcatcgatca
     1321 ttaatcctgt tttttgtcta aatttctctg tcagaccttt ttctttcagc tgatcacaat
     1381 actcagatgt tcttcggcac tgccagacaa ttgcatgata cacaggttct cctgtattct
     1441 tatcccagat gatcgttgtc tctctctggt ttgtaattcc gatggctgca acctgatctg
     1501 gtgtaatatt taacttgttc attgcttcta ctgctactcc aagcatggaa gaccagatct
     1561 catccgcatc atgctccacc catccaggat gaggaaaata ctgtgtaaat tccttctgtg
     1621 caacactgca gatcacacct tgttcattaa ataagataca gcggttactt gttgtccctg
     1681 cgtccagtgc cataatgtat tttttcataa taactacctc ctatgactct tttattatat
     1741 gttcatatta cattatgcat aattttgtgt ctattatttt tctttttttg tgcatttttt
     1801 taaatttaaa aggtcgaaaa aaccctcttt tttcactaaa ttttgttcaa aaagagagta
     1861 ttttcgtgaa tttctaattt tttattttat tattctacaa cagtcgtaaa tttttcccaa
     1921 ggaatatcaa cttctgcgcc ttcattgatg atcgcatcta catgcattaa caatgggaag
     1981 tcatctaaag atacaacgcc tctttctgcc agtttacgta cggcacgagc tgttcttgtt
     2041 gcaataacga tagactctaa agttacaccc tgtaattctt ccattgcctg atcaaatgtc
     2101 agtccacgtc ctaataatgt accaatctta cgagttcttc caccgaagac tgttacatat
     2161 aagtcaccag ctccgtgaat gatgttttct ggtccaccat gaatcagttc taacagatgg
     2221 atcatttctt tgacaccctg tccaaacaat gctgcctgag aattataatg tactgctccg
     2281 atctctccat ctctcttctc tgctaaacca actgcaagag acacacctaa tgcataagca
     2341 ttcttcatgg ctacggcaca ctcaacacct acaacatctg tagaaagact gatgtgatag
     2401 taatctgttg ttaataaaga tttgatgaat tttaatgttt ccatgtcttt tccacagaaa
     2461 gctacatggc tgtcatcatg gtctgctaat tcataacttg tacatggtcc accgatggcg
     2521 ttaaagttaa tatgttttcc ttctggctgt ctctgttcga agatatgtgg ataaggaact
     2581 aaagttccgt catctaaatc taccatacct tttgttactg ttaataaagg tacatcttct
     2641 ggtaatactg gaaggatttc gtcacagaac cagtcaagtc cgaaactgct gactccacct
     2701 aagattaagt ctgctccttc taatgcttct tctaattctt cgatctgata atatttgatc
     2761 ccatcatgaa gagttctctt taatgtgata tggaaattat ccttctttaa tccatcaatg
     2821 atatctctgt ctaaaggtga tcctaccaga cgtacctcgt gtccattttc gaatgctggg
     2881 aatgtgatcg cagagttcat ctgacctgca caaacaaatg taataacgct catttcttat
     2941 ccccctgaaa tttaaatatt ttacttaatc attttattta ttctgctact tttttgcaag
     3001 ctacaacgtc aactcctagt cctaatacgt tagggatcac tacatccccg atcgctacag
     3061 gagctttcat tgttacttta tctaattctt tcatgatatc atagatttta ccctttggaa
     3121 tatcacttgc ggttttgact ggaactcttc ttgcagtctt tgcttctact ctgactgtag
     3181 aagtgatcac tcgtgttgga tttaccagtt ctttttctcc gtaagcggct cctcttgggc
     3241 atccatttcc agtaacctta tatccatttt cttcatcaac ttttaaatga catccttttg
     3301 gacatacgat acaaattaaa tctgtcatct ttatgcacct accttttcta cagatactgt
     3361 aatctcacca ttttctactt tatctaaaaa tgctttcgga ataacgatct tttccatttc
     3421 tcctggtgcc atgtgttctc tcttaaagga agcaagttcc tcaccatttg atcttacttt
     3481 gatcgctaca tctgtaaata cgtttcttgc acggaacata acttcaactt tcttgtctac
     3541 attagatgga cgtactttct gtggcacgat tccgttgacc ccatctccat ttttgatatc
     3601 gatgcatgtc tgatcttttg tttctccatt ttttacgtat tctgcagcac tctttcctgc
     3661 cttctggctt tctgctgtaa cgaagtctac caggtcatga acatgaacga catttccact
     3721 tgcgaagatt ccttctgctg aagtttccat gttttcatat acaactggtc cacttgtggc
     3781 tggatccatt gtaattcctg cagatcttgt taattcattt tctgggatca atccaacaga
     3841 taataataca gtatctacat caaattccat ctctgttcct ggaattggtt gattcttttc
     3901 atcaactttt gatacgataa ctttctcaac tcgtttatca ccctgaatat ctgtgatcgt
     3961 atgtgataaa tacagtggaa tatcataatc atttaagcac tgtacgatgt tacggtttaa
     4021 tccgttagaa tatggacaaa gctctacaca tccaactact tttgcaccct ctaaactcat
     4081 gcgtcttgcc atgatcagtc cgatatctcc ggatcctaag atcagtactt tcttacctac
     4141 catatatcct tcgatattca catatctctg tgctgcacct gctgtaaaca ctccagccgg
     4201 acgatcacca gggatcgcga tggctcctct tgttctttca cggcatccca tatttaagat
     4261 aatggattta ccttcaagtt tcatataacc atttttctta ttcatggcat gtacacattt
     4321 ttctgtttca tctacgtcaa gaaccatcgt atcaagcatc acttcgatat ttgtttcatt
     4381 taacatatca atgaatttac ctgcatattc aggtcctgtt aattcttctt tgaaataatg
     4441 gagtccaaat ccattatgaa tacactgatt taagattcct ccaagttcct gatcacgttc
     4501 tacaaccaga actttttcgg caccattttt acttgcttcc actgctgctg ccagtcctgc
     4561 aggtcctcca cctacaacga ttacatcata cttcattatt tggcacctcc atcttttgtt
     4621 tctcctgtga taatgtttgt tcccaaacga tcctgtggaa tatctgctaa tggtttattc
     4681 agctctcttg cgatgatctc ctgaactctt ggtccacaga aaccaccctg gcatcttccc
     4741 atacctgcat tacatcttct cttgaccgca tcaatggatg ttgctggaat tggacgatgc
     4801 actgcgttga tgatctcacc ttctgtgatc gtttcacaac ggcagatgat ctttccgaat
     4861 gctggattct tttctactaa tttttctctg tcttcatgac ttaaatgttt gaagcggtcg
     4921 atatgtcttt catctacgat atcttctttt gcttctaatg taagtcctga attttcaagc
     4981 atctttaaaa tatctggtgc aattgcacat gcggatgata atcccggtga ctgcattcct
     5041 gcgatattaa taaacccttt gttgttttta tcttcataaa tatggaagtc atgatctgct
     5101 gttcttgctc tgactccagc aaaatttcta atagactctc taaaattaat tcctggtaca
     5161 gattttaatg ctgtatttct cacaaaatca agtccctgtt ttgttgttga tacatcattc
     5221 gctgcacttg gctgtgaatc tggtccaacg atcaggtttc catgaaccgt tggagcaact
     5281 aaaactcctt ttcctttttc atttggacat tggaagatta catgattgac cagatttccc
     5341 tggctcttat ccattaggta atattctcct ttatttggtg taatctcaaa tgtcttttca
     5401 ttacacatct cattaacttt gtctgcgaaa acacctgctg cattgcaaat gtattttgct
     5461 tcgatcactc cgttgtttgt ttctatctta tatgtatcat tttctttttc gattccagaa
     5521 acttctgtat taagtttcac ttcaactcca tttaatacgg ctgtttctgc aagtgccagg
     5581 gctaattccc atggacttac gattcctaca tttggagaac ttaaagccgc aacagctttg
     5641 tctgaaagat ttggttcttc ttttagaagt ttttctttat cccagatttc catatcagga
     5701 actccgtttg tgatacctct ttgttttaat tcttccagtg ttttcttttc ttcttcactg
     5761 aatgcaacta ccaatgctcc gatctgtttg taaggtacat ccagtttctc acataattct
     5821 tttgtataga catttccttt gatattgtac tctgccatct ttgttccagg ctttggatca
     5881 tatcctccat ggatgatcgc gctgtttgct tttgttgttc ctacagaaac atcattttcc
     5941 ttttcaacga ttacagcttt cacattatat tttccaagtt cgtaggcgat agcgcagcct
     6001 gtgatccctg caccgataat tgctacatca tacactgtta ttccctcctg tttttcactt
     6061 gcttttgttg ctttctgact ctatctaacc ataatttttt ttgcctgtca aacccgtcaa
     6121 acgcctattt tatgcggttt tcagagtttt tctattaatc attttatttg attaacaaat
     6181 gatttctttc acggtttttg gattattttt cttaaagtac gtgttcactt gttttttgta
     6241 ttaatcaatt gtataatatt aataatcgct agcttatttt gattagcaat tcgaattaat
     6301 caaaataatt tttgatttat ctattgcaaa tgaaatgtat agaaaagagg gtactattta
     6361 tgggaaagcg aaacaaagta acaacaagag atattgctga atacaccggt gtatcacagt
     6421 ctactgtctc tatgatctta agcaacaaac cacatgtgtc tttttctaaa gaaacaatcg
     6481 ataaagtaag acaaggtgca aaagaacttg gttacaagaa gcctgttgct ggtgtaaaaa
     6541 agaaagaaca ggcacttgct aagagtatca tcgttatttg tccacttctt tcgaatggtt
     6601 attattccat gatcattcat tcgatcacag aacgtgccaa agattatggt tataccgtat
     6661 ttactgtcag tacattgagg gacgtctctc aagaagaact ctatttaaac cttttatctg
     6721 ggtttgaact tgcaggcgtt atttctttat atccaccaac aaagattgca caggcaaatg
     6781 cattatctaa gcaagtccct gttgtttcca tcggtgataa accagatgca tgtcgttttg
     6841 atgcagttga acttgacagt aaaaagcctg gatatattat tgctgaacat cttctctctc
     6901 ttggacataa gcatattact tatatctgta caccgatccg cccaaaagag attggccgga
     6961 tccatcgtct tgctgggctg cgttcttgtt ttaaagatca tggtcttgat ccggaatggg
     7021 tggaggtaaa atctcctacg attgctgctt acggaagata ttctgcggat aattctgaat
     7081 atcaaaacgg atatgatatg gcttctcaag ctttagatga acatacttct tccactgctt
     7141 ttgttgggaa taatgatatg acggcttttg ggatcatggc tgcgatctct gatcatggat
     7201 tccgtgttcc tcatgattac tctgtctgtg gatttgacaa cattccttta tcttccatgc
     7261 ctcagattgc tttaactacg atcgaacatg cttctttggc aaaaggtcgt gaagctgtgg
     7321 atattatata taagaagaac acgcagaaaa atatctctgc caaacatcat tatattatgc
     7381 gaatggaata tgaacctgaa ctgattgttc gtaattctac tgggaaatgc aaaataacaa
     7441 acaaaacaaa agaatgtcac aacatctcct gaaaaatatg acattagtat gcattgattt
     7501 tcatacactc tgtacgttat catataacca tgataaacgt ttatgagtaa caaataagga
     7561 gggcgcatgg ccagcaaact tctaagtaat gaattccaga aacacatttg tctggattat
     7621 gaatgtacta caattgaaga agcaatccac aaagcaggtg atctcttagt tcaagtcgga
     7681 tgtgtaaaac caccctacat taatggtatg atccggcgag aacaagaaac ttccactgat
     7741 atcggattgg gggttatgat cccccatggc acagaagaca ctctgaattt tgttttaaaa
     7801 ccgggaatca gtattgttca gatcccacat ggagtttctt atcatggaaa taccatacat
     7861 ctggtgatcg gcatctcaag ccgtgaggaa gattctctgc aggagctgat gcagattact
     7921 gatattttat taaaacctga aaaatgccat caatttttaa atgcttcttc aaaacaggaa
     7981 ttcattgata tgatcaatca gaattatgta gaagaaggag agttgtaaaa atgaaagaaa
     8041 aaactagtat ttcacaaaaa ctccagaaat ttggttccgt tcttgcagga atggttattc
     8101 caaatatcgg agcgtttatc ggatttggtt taattaccgc attctttttg gaaacaggat
     8161 ggactccaaa tgcaaaactt gcaaaactga tcagtccaat cttaaattac ttacttccaa
     8221 tcttaattgg acatactggt ggtaaaatgt ttggtggcga tcgtggtgga gttatcggtg
     8281 ctctcgttac catgggtgcg atcgtagccg ttgacggaac tccaatgttc ttagctgcca
     8341 tgatcttagg acctgttgcc ggtgtttgca ttaagaaatt tgaccaggca gtggatggaa
     8401 aaatcccttc tggattcgaa atgatcgtaa ataacttctc acttggtatt atcggtgcta
     8461 tcctttgctg tttcgcaatg ttagtattcg gaccagttat ggaaactgta actactgtat
     8521 taacaaatgg tgtaaactgg atcgtagcac ataatgcatt accattatct gcaatctttg
     8581 ttgagccagc caaagtatta ttcttaaata acgcattaga ccatggtatc ttcggaccaa
     8641 ttggttatga acaggtgaag actttaggac attctgcatt attcctatta gtaccaaacc
     8701 ctggtgctgg tcttggatta ttattagctt actggttctt cggaaaagga gaaatgaaag
     8761 aagctgctcc tggaatgatc ttgatccact tcttcggtgg tatccatgaa gtttacttcc
     8821 catatgtatt agcaaaccca ttaacgatca ttggtatgat cgctggtggt atcgttggaa
     8881 caggaacact tacattctta aatgttggta ctgttgccac accatctcct ggtagtatct
     8941 tctctgtcgt agctctttca cctaagaact gtatgttcgg agttttatta agcgtcgtat
     9001 tatcatgtgt tgtatcattc ttattaaata ccattttcgt aaaacgtatg gtagcaaaag
     9061 gtggagacac atctctcgga gaatctgtag taccaaaaga agcaatggga tcttctgcta
     9121 gttcaaatga atctactgca ataacaaatg acagtagtct tggagttgcc ggagtagata
     9181 tcaaaaatat taagaaaatc gttgttgcat gtgatgctgg aatgggatct tctgcaatgt
     9241 ctgc
//
LOCUS       DAOMUE010000044         9193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1897_length_9193_cov_2.619063, whole genome shotgun sequence.
ACCESSION   DAOMUE010000044 DAOMUE010000000
VERSION     DAOMUE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9193
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1897_length_9193_cov_2.619063"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="PLC71_03480"
     CDS             <1..909
                     /locus_tag="PLC71_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRM01740.1"
                     /translation="LQDLTLLDRFLFAEVMEDAETFENVLSIILGQDISIKGHPQSEH
                     EKRTSPLKRQVRLDVWAEDDTDAVYNIEAQKENTKNLPHRSRFYQALIDSKLLDPGEI
                     DFSKMKDCYSIIIAPFDLFGEGLYQYTFQMNCNETGQPLNDGAIRIFLNTHGQNPEDI
                     SSELKELLYYMEHTTEDIFCSTPRLQEIKKHVNIVKSSEEIGVKYMQEWEEKILEKRK
                     ARAEGLAEGLAEGLAEGRAEGLAEGDYFRLIQQIKKKIEKSKNLIQIADELEETPENI
                     ESLYHCIKDHFTLENKDIYKLYKDCN"
     gene            complement(964..2913)
                     /locus_tag="PLC71_03485"
     CDS             complement(964..2913)
                     /locus_tag="PLC71_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM01741.1"
                     /translation="MEDRRSIAKKAAIGVALVIAAAGAVLFYKNNIAMNPGDTLLKYM
                     SYAEDGKYEKMYDLLNEESQKSISKEEFIKRNKNIYKGIGVQTIDADVTSKKRSTTVT
                     YHVKMQTNAGIITYNNRTDFVKENHRYRIDWDDSVIFPQLGAEDKVRVKTLYAKRGRI
                     KDAQGNALAVQGKIYSVGFVPGKMDGNSVKLAAKKLGLSKEEIQKKLDQKWVTDDSFV
                     PLIKLKEYSEDLLDVKGIIVSTETGRIYPLGEAAAHLIGYMQNGEGKAGLEKLYDDQL
                     SGTNGLEIYIEDSNGQKKQSLAVRSQTDGKDLTTTINSSLQKAIYEQYKNDKSAHVAL
                     NPSTGEVKALVSTPSYDAQAFILGMSQKKWNVINSDQRLPMQNRFKATFAPGSSIKPI
                     IAAIGLSQNKFRANDDFGNSGKRWQKDKSWGGYYVTTLHDYNGHNLQNAMIYSDNIYF
                     AKAALKIGKDTLKDQLDNLGFGESLKFTFGLNASSYGSEGFTSDIQLADSGYGQGKMM
                     VNPVHMAAIYTAFSNNGNMLKPYLVKENGSKKQVLKETIFTKQAVNTVNEAMRQVIRD
                     PSGTGHAANIEGVDLRGKTGTAEIKQSQTDTKGTEIGWFVTIMPATNNREALELVSMT
                     ENVKKRGGSGYVVNKTKKIMEEYLQ"
     gene            complement(2961..3755)
                     /locus_tag="PLC71_03490"
     CDS             complement(2961..3755)
                     /locus_tag="PLC71_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRM01742.1"
                     /translation="MDKIAVAQTTSSGNWRENIEKAKQYIKKATEEEAVMIIFPEYFM
                     NYYPDAEHNYTKKAQSLHGEFVQAMKMLAKEFKMWIIFGMNEQTVDETKNYNTMVILN
                     DLGELVSDYKKTHLFNAYKWNESMNTLKGDCLFTPIKTPAGVIGLGICYDLRFPELAR
                     YEALSGTQVMLYPAAWVKGKGKFMQWETLLRARAIENEMYVLGCCHYSKEHYMGRSTG
                     FAPDGTRLYLGEEKEELLYAQIDKEQIQNIRTANPVFENRRKDLYD"
     gene            3943..4015
                     /locus_tag="PLC71_03495"
     tRNA            3943..4015
                     /locus_tag="PLC71_03495"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3976..3978,aa:Lys,seq:ctt)
     gene            4159..5334
                     /locus_tag="PLC71_03500"
     CDS             4159..5334
                     /locus_tag="PLC71_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRM01743.1"
                     /translation="MSSNHKFESNKKYFTISIYTIAVILIGCVIFRFITQWSNTSKLI
                     SQLWEILFPFFMGFLIAYILNPVVAFFQRNVSMKLLKKKSANAQRGLAILISYLVMVG
                     FIIVCLRFIIPQLYDSIRELSLMIPDIYKSIMSIFEHYRENSTDMFPGQIADMIETKT
                     LPKLFELTNNLLTNIVPMLYEASMSFAKGLFNLFIAFIVSIYMTIDKFILKNAGKRLV
                     LAIFNENFSYRVLRTLKDCHNIFSGFIIGKSIDSLIIGLLAFVAMTILKLPYTVLISV
                     IIGVTNMIPYFGPIIGAVPGAFILFIVSPTKCLIFIIMVFVLQQFDGLILGPKILGES
                     TGVRPLLILFSITVGGAYFGPLGMFLGVPFFATVQFLIGNWVDYRLYKKNIKLEKEA"
     gene            complement(5389..7176)
                     /locus_tag="PLC71_03505"
     CDS             complement(5389..7176)
                     /locus_tag="PLC71_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRM01744.1"
                     /translation="MNILNIEHISKIYGEKVIFDDVSLGIHSGDKIGVIGVNGTGKTT
                     LLKIIAKINEPDKGQIICGNGIRVSYLPQNPEFPKKQSILEYVMDGKEHQDWKTESEA
                     KTILTKLGIYDFDEGCDHLSGGQKKRVALARTLVDPTEVLILDEPTNHLDNDMVLWLE
                     EFLNSFRGVLIMVTHDRYFLDRVTNKIVEIDKGKLYEYDTNYSGFVELKVQREEMELA
                     TERKRQSLLRVEMEWMKQGIKARGTRQRARTERFEELKNAKGPSMQQNVEMDSISSRL
                     GKTTIELEHISKGFGDKHLINDFSYIVLRDDRIGFIGPNGCGKSTLMKMIMGILKPDE
                     GNITIGDTVKIGYFAQENEDMTGDIRVIDYIRNVAEYIQTTKGQASASQMLDRFLFPP
                     ELQYTPLDKLSGGEQRRLYLLKVLMEAPNVLILDEPTNDLDIQTLTILEDYLDTFAGI
                     VITVSHDRYFLDRIVNRIFAFEEGGHLKQYEGGYTDYLEKVKPIAKQEKSKPEKKENN
                     GKKFRKEHQKKLKFTYKEQKEFETIDDDIAKLEEKIEQLDEEIMENATNSGKLAELTQ
                     QKEETEEALNEKMDRWVYLNDLAEQIANQ"
     gene            complement(7181..7498)
                     /gene="trxA"
                     /locus_tag="PLC71_03510"
     CDS             complement(7181..7498)
                     /gene="trxA"
                     /locus_tag="PLC71_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRM01745.1"
                     /translation="MEYTFTSDNFEEEVLKSDVPVLVDMFATWCGPCKMMAPVVAQLA
                     EEYEGTVKVGKLDIDQNVDIVAQYKIMSVPTFLVIKDGEVKAKLIGAVSKEELVEAID
                     QAK"
     gene            complement(7681..8325)
                     /gene="trmB"
                     /locus_tag="PLC71_03515"
     CDS             complement(7681..8325)
                     /gene="trmB"
                     /locus_tag="PLC71_03515"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRM01746.1"
                     /translation="MRLRNVPGARETIIENQFSIQQPEQMKGKWAEVFKNDHPIHIEV
                     GMGKGQFIIEMARRNPEVNYIGIEKYSSVLVRAVEKLEDFEQDNLRLIRMDAENIEEV
                     FDKDEVDRIYLNFSDPWPKDRHAKRRLTSTRFLERYDNILTPEGRVMFKTDNKDLFDF
                     SLEQVEEAGWILENHTYDLHHSEYNEGNVMTEYEEKFSAKGNPICRLVAYRHAK"
     gene            8445..8894
                     /locus_tag="PLC71_03520"
     CDS             8445..8894
                     /locus_tag="PLC71_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HRM01747.1"
                     /translation="MPMEIERKFIVHNLPDNLDSYPHSEIEQGYLCMEPVIRIRRMNK
                     QYFLTFKSKGLMARTEHEFPLDKAAYEHLLPKVDGILISKTRYKIPDEHGFIIELDVF
                     HGEYEGLVMAEVEFPSEEVAVEYEGPEWFDDEVTFDSRYQNNNLANK"
     gene            complement(8979..>9193)
                     /locus_tag="PLC71_03525"
     CDS             complement(8979..>9193)
                     /locus_tag="PLC71_03525"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRM01748.1"
                     /translation="KTDEECMDFVIGKIKELSERVGIPKTLSELGVENPDFETLAENS
                     MKDACAGANPVFFDKELLIKLFKKIA"
BASE COUNT         2438 a         1887 c         1519 g         3349 t
ORIGIN      
        1 ctgcaagatt taactttact tgaccgcttt ttatttgctg aagttatgga agatgcagaa
       61 acttttgaaa acgtattatc tattattctt ggccaagaca tatccattaa aggacatccg
      121 caatccgaac atgaaaagcg tacatcgcct ttaaaacgtc aggtgcgcct tgatgtatgg
      181 gctgaggatg atacggatgc tgtatacaat atagaagcac aaaaggaaaa taccaaaaat
      241 cttcctcaca gaagtcgttt ttatcaggca ttaattgatt cgaaacttct ggacccggga
      301 gagatagatt tttcaaagat gaaagattgt tattctatta ttattgcacc ttttgatctt
      361 tttggggaag gattatatca atatactttt caaatgaact gcaatgaaac tggtcaacct
      421 ttaaacgatg gtgctatacg tatttttcta aatactcatg gtcaaaatcc cgaagatatc
      481 agttccgaat taaaagaact tttatactat atggaacata ctaccgaaga tatcttttgc
      541 tctacaccac gccttcagga aataaaaaaa catgtcaata tcgtaaaatc cagcgaagag
      601 attggagtga aatatatgca ggaatgggaa gaaaaaattc ttgagaaacg aaaagcacgt
      661 gctgagggac ttgctgaagg acttgctgag ggacttgctg agggacgtgc tgaaggactt
      721 gctgagggag attactttcg attaatacaa caaatcaaga aaaaaattga aaaatccaag
      781 aatttaattc aaattgctga tgaattagaa gaaacacctg aaaatattga gtctttatat
      841 cattgcatca aagatcattt tactttggaa aataaagata tctataaact ttataaagat
      901 tgtaattaga ataggatttc gatgcctgaa atcatcaatc atggaagaat acctgcagct
      961 gatctactgc aggtattctt ccatgatctt ctttgtttta ttgaccacgt atccacttcc
     1021 gcctcgtttc ttcacgtttt ctgtcatgga taccagttcc aatgcttccc tattgtttgt
     1081 tgctggcatt atggtcacaa accagccgat ttctgttcct tttgtgtctg tctgtgattg
     1141 tttgatctct gcagttcctg tctttcctcg aagatctacc ccttctatgt tcgctgcgtg
     1201 tcccgtacca gagggatctc tgattacttg tcgcatagct tcatttacgg tattcacagc
     1261 ttgtttggta aatatagttt cctttaatac ttgttttttt gatccgtttt ctttgaccag
     1321 atacggtttc agcatattac cattattaga aaatgctgtg tagattgctg ccatatgtac
     1381 cgggtttacc atcattttcc cctgtccata tccactgtca gctaactgaa tatcggaagt
     1441 aaatccttca cttccatagg atgaagcgtt tagtccaaat gtaaatttca gactttctcc
     1501 aaaacctaag ttgtcaagct gatcctttaa ggtatctttt cctatcttca gggctgcttt
     1561 tgcaaaataa atattgtcag aatagatcat tgcattttgc agattatgcc cattataatc
     1621 gtgcaatgtt gttacgtaat aaccacccca gcttttatcc ttctgccagc gttttccact
     1681 gtttccaaag tcatcatttg cacgaaattt attctggctt agtccgatcg ctgctatgat
     1741 cggtttgatg gatgatcctg gtgcaaatgt tgctttaaag cgattttgca ttggcagtct
     1801 ttgatcgcta ttgatcacat tccatttctt ctggctcatt cctaagataa atgcctgtgc
     1861 atcataagac ggagtactta caagtgcctt gacctctcct gttgaaggat tcaatgcaac
     1921 atgagcactt ttatcatttt tatactgttc gtaaatagcc ttttgcaaac tactgttgat
     1981 cgttgttgtt aaatcctttc catctgtctg agatctgacc gccagtgact gttttttctg
     2041 tccattagaa tcttctatgt aaatctctaa gccattcgtg ccagaaagct gatcatcata
     2101 taatttctca agtcctgctt ttccttcgcc attttgcata taaccgataa gatgtgctgc
     2161 tgcctctcca agtggataga ttctcccagt ttctgtggaa acaatgatcc ctttcacatc
     2221 gagaagatct tctgaatatt cttttaattt gatcaatggt acaaaagaat catccgtcac
     2281 ccatttttga tcaagtttct tctgaatctc ttctttggat aatcctaatt tctttgccgc
     2341 aagttttaca gaattaccgt ccatcttccc aggaacaaat cctacagaat agatcttacc
     2401 ctgtacagca agtgcatttc cctgtgcatc tttgatcctt cctcttttgg catataaagt
     2461 ttttactctg actttgtctt ctgctcctaa ttgcggaaaa ataacggagt catcccaatc
     2521 tatgcgatat ctatgatttt ctttgacaaa atctgtacgg ttattatagg taataattcc
     2581 agcatttgtc tgcattttta cgtgatatgt cacggttgtt gatctcttct ttgaagttac
     2641 atcagcatcg atcgtttgga ccccgattcc cttataaata tttttatttc tcttgataaa
     2701 ttcttctttt gaaatacttt tttggctttc ttcattcaaa agatcgtaca tcttttcata
     2761 ctttccatct tctgcatatg acatatattt taataatgtg tcacctggat tcatcgcaat
     2821 attattctta tagaataata cagctcccgc agctgcgatc accagtgcga ctcctattgc
     2881 tgccttcttt gctatacttc ttctatcttc catatgatac tacctctttc tgattcctac
     2941 cttgatctgt attattttat ttaatcgtag agatcttttc tcctgttttc aaagacagga
     3001 ttggctgttc ttatattttg aatctgttct ttatctatct gtgcatagag cagctcttcc
     3061 ttttcttctc ctaaatacaa tctggttcca tctggtgcaa atccagtact tcttcccatg
     3121 taatgttcct tactgtaatg acaacatcct aaaacataca tctcattttc aatagctctt
     3181 gcccgaagaa gtgtctccca ctgcataaat tttcctttac ccttaaccca tgccgctgga
     3241 tacaacatga cctgagttcc agataatgct tcatatcttg caagttccgg aaatctgaga
     3301 tcataacaga ttccaagtcc gatcacacct gcaggtgtct taatcggtgt aaacaaacaa
     3361 tcccccttaa gcgtattcat tgattcattc catttatatg cattaaacaa atgtgtcttt
     3421 ttataatcag acacaagttc tcccaggtca ttcaggatta ccattgtatt gtagttcttt
     3481 gtttcatcca cagtctgttc attcatccca aaaatgatcc acatcttaaa ctcttttgca
     3541 agcatcttca ttgcctgtac aaattcgcca tgcaagcttt gtgccttttt tgtatagttg
     3601 tgttcagcat ccggataata attcatgaaa tattctggaa aaatgatcat aactgcctct
     3661 tcttctgttg ctttctttat atattgtttt gctttttcta tattttctct ccagtttccg
     3721 gaagatgttg tctgtgcaac cgctatttta tccatgaaat tttatcccct ttccagtctc
     3781 tagaatacca tatttcgggc tttttttgta ccgttattaa ctttctttaa attttttgta
     3841 aaaaaaaata aaaaaaatta aaaataatgc ttgcatttct taaaacattc gtatataata
     3901 aatcttgcgt taaggaagcg cagtaaaaac aacataattt aagcgtgatt agctcagttg
     3961 gtagagcacc tgactcttaa tcagggtgtc gagggttcga acccctcatc gcgtactaaa
     4021 gcactgtttt tggttgattt tgtagccggg gatggtgttt ttcttttatt taaatcttat
     4081 attgtcttgt aactgatctt ggaaacgtct ataatagatg atattactga ttaactatca
     4141 ttctaagagg agcctcaaat gtcaagcaac cacaaattcg aatcgaataa aaagtatttt
     4201 acgatttcta tctataccat tgctgttatc ctgatcggat gtgtgatctt ccgctttata
     4261 acacaatggt ctaatacaag caaactgatc tctcaattat gggaaatctt atttccattt
     4321 tttatgggat ttctgattgc ctatatttta aatcccgtcg tagctttctt ccaaagaaat
     4381 gtatcaatga aacttttaaa aaagaaatct gccaatgcac agcgtggact cgctattttg
     4441 atcagttatc ttgtcatggt tggttttatc attgtatgtc tacgatttat cattccacag
     4501 ttatatgaca gtattcgtga actatccctg atgattcccg atatttataa atcgatcatg
     4561 tcaatctttg aacattatcg tgagaacagc acagatatgt ttcctggtca gattgccgat
     4621 atgatcgaga caaagacatt accaaagctt ttcgaactga ccaacaatct tctgaccaat
     4681 atcgtcccta tgctttatga ggcttccatg tcttttgcca agggattatt taacctgttt
     4741 atcgcattta ttgtttctat ttatatgacg atcgataaat ttattttaaa aaatgctgga
     4801 aaacgattgg ttcttgcaat ctttaatgaa aatttctctt atcgtgtact tcgcacttta
     4861 aaagattgtc ataatatttt ttctggtttc attatcggaa aatccattga ttctctgatc
     4921 attggtttac tggcttttgt tgcgatgact attttaaaac ttccttatac cgtattgatc
     4981 agtgttatta tcggtgtcac aaatatgatt ccttattttg gaccgatcat tggtgcagtt
     5041 cccggtgctt ttattctttt tatcgtaagc ccgacgaagt gcctgatctt tattatcatg
     5101 gtttttgtcc tacagcagtt tgacggtctg atccttggac ctaagatcct tggagaatct
     5161 accggagttc gtccactact gattttattt tccatcactg tcggtggcgc ttatttcggt
     5221 ccacttggaa tgttccttgg agttccgttt tttgctacag ttcaattttt gatcggaaac
     5281 tgggtggatt atcgattgta taagaagaat attaagttag aaaaagaagc ttaacataat
     5341 aagaggattg actcatatga tattttgtga gtcaatcctc ttttattgtt attgattcgc
     5401 tatctgttct gccagatcat ttaaatatac ccaacggtcc atcttttcat tcaaagcttc
     5461 ttctgtctct tctttttgct gcgtaagctc tgctaattta ccagaattcg ttgcattttc
     5521 catgatctct tcatctaact gttctatttt ttcctccagt ttcgcgatat catcatcgat
     5581 cgtttcaaat tctttttgtt ctttgtatgt aaacttgagc ttcttttggt gttctttccg
     5641 gaatttcttt ccattatttt cttttttctc tggtttactt ttctcttgtt tcgcaattgg
     5701 ctttactttc tccagataat ccgtataacc gccttcgtac tgttttaaat gaccaccttc
     5761 ttcaaacgca aagatcctgt ttacaatacg atcaagaaaa tatcggtcat gagatactgt
     5821 gatcacgatt cctgcaaacg tatccagata atcttctaag atcgttaatg tctggatgtc
     5881 aaggtcgttg gtcggctcat cgaggatcag aacatttggt gcttccatca atacttttaa
     5941 taagtagaga cgtctctgtt ctcctccaga aagtttatca agcggtgtgt attgaagttc
     6001 tggcgggaac aagaaacggt ctagcatctg ggatgcactt gcctgccctt ttgttgtctg
     6061 gatatattct gcgacatttc tgatataatc aatgacacgg atatctcctg tcatatcttc
     6121 attttcctgt gcaaaatatc cgatctttac ggtgtcaccg atcgtaatgt ttccttcgtc
     6181 cggctttaag attcccatga tcatcttcat caaagttgat tttccacagc catttggtcc
     6241 gataaatcca atcctgtcat ctcttaatac gatgtaagag aaatcattga tcagatgttt
     6301 atcaccaaat cctttggaaa tgtgttctaa ctcgatcgtt gtctttccaa gacggctgct
     6361 gatcgaatcc atctcaacat tttgctgcat ggatggacct ttggcattct taagttcttc
     6421 aaaacgttcc gttctggctc tctgtctcgt tcctctcgct ttgattccct gtttcatcca
     6481 ttccatttcc acgcggagta aactctggcg cttacgttct gtcgctagtt ccatctcttc
     6541 gcgctggact tttaactcta cgaatccaga atagttggta tcgtattcgt aaagctttcc
     6601 tttgtcgatc tctacgatct tattcgttac acgatctaag aaataacggt catgggttac
     6661 catgatcaat acacctctaa agctgtttaa gaattcttcc agccatagaa ccatatcgtt
     6721 atctaagtgg ttggttggct cgtcaaggat cagtacttcg gttggatcta ctaaagttct
     6781 tgcgagtgca actctcttct tctggcctcc ggataaatga tcgcatcctt cgtcaaaatc
     6841 atagattcca agttttgtaa ggatcgtctt tgcttcactc tccgtcttcc agtcctgatg
     6901 ttctttccca tccatcacat attcaagaat gctttgtttc ttcggaaatt ctggattctg
     6961 tggaagataa gatacacgga ttccatttcc acagataatc tgtcctttgt ctggctcgtt
     7021 gatcttggca atgatcttta ataatgtagt ctttcctgta ccgttgaccc cgatcacacc
     7081 gatcttatcg ccactgtgga ttccaagaga tacgtcatca aaaatgactt tctctccata
     7141 aattttgctg atatgttcaa tatttaatat attcatctta ctactttgcc tgatcgattg
     7201 cttcaactaa ttcttctttg gatactgctc cgatcagttt tgccttaacc tctccatctt
     7261 tgatcactaa aaatgttgga actgacatga tcttgtactg tgctacgata tcaacattct
     7321 gatcgatatc aagttttccg acttttacag ttccttcata ttcttcagct aattgtgcta
     7381 ccactggtgc catcatctta caaggtccgc accatgttgc aaacatatct actaatactg
     7441 gcacatcact ttttaatact tcttcttcaa aattatcact tgtaaatgta tattccataa
     7501 taattcacct atcctttgct atttatttaa acatcgtttt gcttcgtcta gtgactgaaa
     7561 aaattgacag acccatctgt tcatgcatgg atgcaaatat agaaacaact ggatcttgca
     7621 tcgaaacttt ccttgtttca tgattatatt accacttttt gtttatttta tgcaatcctt
     7681 ttacttggca tgacgatatg cgactaaacg acagattgga tttcctttag ctgaaaattt
     7741 ctcctcgtat tctgtcatga catttccttc attatattcg ctgtgatgaa gatcgtatgt
     7801 gtgattttcc aggatccatc ctgcttcttc tacctgctct aaggagaagt caaataagtc
     7861 tttgttatct gtcttaaaca taactcttcc ttctggtgtc agaatattat catatcgttc
     7921 taaaaatctt gtagatgtca atcttctctt tgcatggcgg tcttttggcc atggatcaga
     7981 gaagtttaaa tagatcctgt ctacttcatc tttatcaaac acttcttcga tattctctgc
     8041 atccatacgg atcaaacgaa ggttgtcctg ttcaaaatct tctaatttct ctacagctct
     8101 tactaagaca cttgaatatt tttcgattcc aatatagttt acttctggat ttcttcttgc
     8161 catttctata ataaactgtc cttttcccat tcctacttcg atgtggatcg gatggtcatt
     8221 tttgaatacc tctgcccatt ttcctttcat ctgctctggc tgctggatgg aaaactgatt
     8281 ttctatgatc gtctctctcg ctcctggtac atttcttaaa cgcatttata ttcatctcct
     8341 ttgtcttttt ctcgaatctt tgttgattat atcatagacg atgggtataa taaagaaaaa
     8401 gtatgcgcct taaaaaggct taggatctgc acttcaagga ggaaatgccc atggaaatcg
     8461 aaagaaaatt tatcgtccac aacttacctg ataatctgga ttcttatcca cactctgaga
     8521 tcgaacaggg ctatctttgc atggaacctg tgattcgtat ccgcagaatg aataagcagt
     8581 atttcttaac tttcaagagt aaaggattga tggctagaac tgagcatgaa tttccacttg
     8641 ataaagcagc ttatgagcat ctgcttccaa aggtggatgg gattttaatt tctaagactc
     8701 gttataagat tccggatgaa catggattta ttattgagct ggatgtcttt catggagaat
     8761 atgaggggct tgtaatggct gaggtggaat ttcctagtga ggaagtcgct gtagagtatg
     8821 aagggcctga atggtttgat gatgaggtta cgtttgattc tcggtatcag aataataatt
     8881 tggcgaataa atagagaata tcgttttcgt atattcgtga aaataaatct gcattgagtt
     8941 aaatgatatc aatgcagatc tttttcattt tataaaactt aggcaatttt cttaaacagt
     9001 ttgatcaata attctttatc aaaaaatact ggatttgctc ctgcgcatgc atctttcatg
     9061 gaattttctg ctagggtttc aaaatctgga ttttctactc caagttctga caatgtttta
     9121 ggaattccta ctctctctga taattccttg atcttaccaa ttacaaagtc catacactcc
     9181 tcgtctgtct ttc
//
LOCUS       DAOMUE010000045         8912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_1999_length_8912_cov_2.380490, whole genome shotgun sequence.
ACCESSION   DAOMUE010000045 DAOMUE010000000
VERSION     DAOMUE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8912
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1999_length_8912_cov_2.380490"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>680
                     /locus_tag="PLC71_03530"
     CDS             <1..>680
                     /locus_tag="PLC71_03530"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRM01749.1"
                     /translation="YKIIYVAPERLETEGFINFALNSGVKISMLAVDEAHCVSQWGQD
                     FRPSYLKILEFLKKLPYRPVLTAYTATATAEVRDDIMDILNLRDPFVLTTGFDRENLY
                     YAVKRPRDKYRELLSYLKEKEEKMPGSSGIIYCLSRKNVEEVCYQLREDGFSVTRYHA
                     GLSDEERKENQEDFIYDRKQIMVATNAFGMGIDKPDVRFVVHYNMPKNMESYYQEAGR
                     AGRDGEPA"
     assembly_gap    681..690
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <691..1517
                     /locus_tag="PLC71_03535"
     CDS             <691..1517
                     /locus_tag="PLC71_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HRM01750.1"
                     /translation="NRFLIENGEENEELDAITKQIVMERDWERLRQMTFYCYTKECLR
                     HYILNYFGEQTSGYCGNCLNCLTEFETVDATMEAKAIIQCIRDCYRDFGLSTIVDILK
                     GSKSQKVLSRHLDENSQYGALASESIARIRQIINEMLVYGYLEMTDDEYSVLSVADIT
                     KIDDNWNFEIKIPKEVEKEKEERKIQTKKRKKAGAVAALAEEDTELFEALRVLRRMIA
                     SEQKIPPYMVFSDKTLVHMSTMKPSNDEEMLEVNGVGEHKLQKYGERFLNEIQKYK"
     gene            1549..2577
                     /locus_tag="PLC71_03540"
     CDS             1549..2577
                     /locus_tag="PLC71_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3878 family protein"
                     /protein_id="HRM01751.1"
                     /translation="MNYFETNLEEISRSIQLLAQIFEYQVFELQVEEKDGNQTGYVPY
                     MMNDAIECYLSFHGLRISGKYDKDYEGEMWAQLEEREGRYGLIVHQGEESVFTMWFDE
                     IMEHTNCYRYHEIGHFWRDGAEQWRQLVYMIGTIREKYRFLGEEVCNDQEMEIMSLIE
                     FAPFYYYFPINEDPEEWYDKTEEGLECMRNLAMQAGDKDYLKWIDKYEKHPTKRMEMT
                     LAKKLQDPKRQDLYELICEKVCNASDPYPARNYGERIDQKIQRYREQVDKKLKEQGFT
                     GTYPQYESEHLSVCVTEEHPFTILESEDFKFKIQLMISECREKHPRKNAGFFKGYQRK
                     GKIKRLDF"
     gene            complement(2613..3194)
                     /locus_tag="PLC71_03545"
     CDS             complement(2613..3194)
                     /locus_tag="PLC71_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRM01752.1"
                     /translation="MNTLTAEKRDLKTKAKKLRKEGYVIGNLFGRDIENSIPLKVDSR
                     EAAKFLKDNKKGAHITLLVDGENVDAIVKEIDYNAMKNETMFINFQALVADEKIHTTA
                     AIELLNEDAVQNGIVEQSLSEIEYKAFPADIVEKVEIDLTQYNVGDMIHVKDLPLAAN
                     SKISLVTPEDTLVVNIAEPEAVPEDEDEEAAEE"
     gene            3464..4078
                     /locus_tag="PLC71_03550"
     CDS             3464..4078
                     /locus_tag="PLC71_03550"
                     /inference="COORDINATES: protein motif:HMM:NF028234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HRM01753.1"
                     /translation="MIYDFAVIGGDMRQVYLANYLKNQRYQVITYATKKEGSECKEGK
                     SFENVIREAKCMIGPVVFSKNGEITGSSSNKKMSVETLFQEMTKEQILFGGCINEEIR
                     HECEQKEIKAFDFMKMDDVAIYNAIATAEGAIMEAIKMQPINLHKSRCLVLGYGRCAK
                     VLAAKLKGMDAQVTVCARNKTARSLAKSFGLEVMDFTELKRRIC"
     gene            4199..4336
                     /locus_tag="PLC71_03555"
     CDS             4199..4336
                     /locus_tag="PLC71_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01754.1"
                     /translation="MAEKLGICAKLCPSLPGIYSPKSSGIMLAKKVLEISGEIKHGIE
                     K"
     gene            4320..4910
                     /locus_tag="PLC71_03560"
     CDS             4320..4910
                     /locus_tag="PLC71_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HRM01755.1"
                     /translation="MVLKNKRIGVAMTGSFCTYTKAFEQIDRLLEEGAILQTIFSDIS
                     QHIKCRFGKGEEFVKRAHDMTGNEPMKTIEEAEIIGPTGCLDILILMPCTGNTMAKLA
                     NGITDTPVLMAAKAQLRNEKPVVISMASNDALGMNLKNIGNLLNHKHIYFVPFGQDDP
                     VKKPNSMIADTRLLIPTILQALKGRQYQPVLIDHKK"
     gene            4926..6782
                     /gene="asnB"
                     /locus_tag="PLC71_03565"
     CDS             4926..6782
                     /gene="asnB"
                     /locus_tag="PLC71_03565"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRM01756.1"
                     /translation="MCGIAGFLNPRNNDKKTEKEIYQILKKMNEVQRHRGPDDEGIFL
                     ENGCALGHVRLSILDLYRGHQPMCKKKEGNTYAIILNGEIYNMKTLKEQLIKEGEMFS
                     TTSDTEVVLTGYIRYGISYIEELNGIFSIALWDGKRKKLYLIRDRVGVKPLFYTKRGD
                     TLIFASEIKGLFAYPGVKPVINREGLCEIFGLGPAKSYGKGVFKDIYEVLPGHFLEYD
                     REGLKDRAYWELKAKEHTDSEKDTIEHTRWLVKDAVEMQMLSDIPISTFLSGGVDSSL
                     VTAICAKKLQKEGKNLNTFSFDFVNNHKYFKSNAFQPSEDRPWVDKMVDYAKTDHRYL
                     ECDNENMIENLYKAVDARDLPCMADVESSMLYFCSKVVKYNKVTLTGECADEIFGGYP
                     WFHKEECFKAEIFPWSMDMQPRKMLLNDDIIQKVDLESYARTAYQKTINETPKLYGED
                     QIEARRRQISYLNLRWFMVTLMDRMDRTSMHCGLEARVPFADHRIVEYLYNVPWELKC
                     LNGVVKGLLRAAGEGILPDEVLYRRKSPYPKTYDPNYEKILSKELLKVMAKKGAPIKE
                     LIDAYKLSRFLDSPKDYGKPWYGQLMAGPQMIAYLLQVNYWLEKYQITLNLS"
     gene            complement(6783..7727)
                     /locus_tag="PLC71_03570"
     CDS             complement(6783..7727)
                     /locus_tag="PLC71_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM01757.1"
                     /translation="MENLIFSLNATVPVFLMMILGLVFKKIGWIDDIFASKMNKFVFM
                     VPLPVLVFEDLATVDFSKVWDVKFVLFCFIVTMISITISFLISCLLGDKTVRGEFIQA
                     AYRSSAALLGIAFIQNIYGNAGMAPLMIIGSVPLYNMMAVVVLSVFKPGQSGLDHTVV
                     KKTVKGIVTNPILIGIVAGLVWSALKIPMPAIPKKVISSIGNVATPMGLMAMGATFDI
                     KKAFGKIKPSAIAAFMKLIGLCAIFLPIAVKLGFRTDKLVAILIMLGSATTVSSFVMA
                     KNMGHEGTLSSSVVMLTTMFSAFTVTGWLYILRHFMLI"
     gene            complement(7730..8434)
                     /locus_tag="PLC71_03575"
     CDS             complement(7730..8434)
                     /locus_tag="PLC71_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HRM01758.1"
                     /translation="MDRWIHFNMAIVGGFLGGFAILNHHELFGSAQTSNLISVFADLA
                     GHPDANFFVRLLGVFIYMFALSLTVILPKFTRLHLPYVSLFIDAMAALIVAFLPSDLN
                     DFIALYPLYFAMAIQWCSFKGGDGFTCSTIFSTNNLRQFTTSMTEYLCSKDPDALRKW
                     KFFASVLLCFHFGVIVSYLSCKNFGHTGSLVCFLPVFSGFCLVWTRKHFVAVKHFVYH
                     YLITPKKSSAYTTESE"
     gene            8738..>8912
                     /locus_tag="PLC71_03580"
     CDS             8738..>8912
                     /locus_tag="PLC71_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HRM01759.1"
                     /translation="MDKTNGKLTVYFEKPFWVGIFERTEDGKLSVAKVTFGVEPKDYE
                     VQEYIQKHYFRLKF"
BASE COUNT         3171 a         1461 c         1885 g         2385 t
ORIGIN      
        1 gatataagat catctatgtg gcaccagaac gcttggaaac agaaggtttt atcaattttg
       61 cgctaaatag tggagtgaag atatcaatgt tggcagttga tgaggcacat tgtgtgtctc
      121 agtgggggca ggatttcaga ccaagttatc taaagatttt ggaattttta aagaaacttc
      181 cgtatcggcc tgtactgact gcatatacag caacagcgac agcagaagtc agggatgata
      241 ttatggatat tttgaacctg agagatccat ttgtattaac gacagggttt gaccgggaga
      301 atctttacta tgcagtaaaa cgtccaaggg ataagtatag agaattacta agttatttaa
      361 aagaaaaaga agaaaagatg ccaggaagca gcgggatcat ttactgttta tctaggaaaa
      421 atgttgagga agtctgctat cagttaagag aagatggatt ctccgtgaca agataccatg
      481 caggattatc ggatgaagaa cgaaaggaaa atcaggaaga ttttatctat gacagaaagc
      541 agatcatggt agcgacaaat gcctttggaa tgggaatcga caagccggat gtccgttttg
      601 ttgttcatta taatatgccg aagaacatgg aaagttatta tcaggaagca ggaagagcag
      661 gacgtgatgg agaacctgcg nnnnnnnnnn ccaatcgttt tctgatcgaa aatggagagg
      721 agaatgagga attagatgcg atcacaaagc agatcgtgat ggaaagagac tgggaaagac
      781 ttcgtcagat gacattttac tgctatacga aggaatgttt acgtcattat attttaaatt
      841 attttggaga acagacaagc ggatactgtg gaaactgctt aaactgtctg accgagtttg
      901 aaacggttga tgcaacaatg gaagcgaaag ctatcatcca atgtattaga gattgttatc
      961 gagatttcgg actatccacg atcgttgata ttttaaaagg ttcaaagagc cagaaagtat
     1021 taagccgtca tttagatgag aattcacaat atggagcatt ggcatcagaa agcattgcaa
     1081 ggatacgaca gatcatcaat gaaatgttag tctatggata tctggaaatg acagatgatg
     1141 aatattcggt tttaagcgtt gcagatatca ctaagattga tgataactgg aactttgaga
     1201 ttaagattcc aaaagaagtc gaaaaagaga aagaagaacg caagattcag acgaagaaac
     1261 gtaagaaagc aggggctgtg gcagcacttg ctgaggaaga tacagaattg ttcgaagcat
     1321 tacgtgtact tcgaagaatg atcgcatccg aacagaaaat cccgccatat atggtatttt
     1381 ccgataagac actggtacat atgagtacga tgaaaccttc taatgatgag gaaatgttag
     1441 aagtcaatgg agtcggagaa cataagcttc agaaatatgg cgaacgtttt ttaaatgaaa
     1501 ttcagaaata taaataaaat acatagataa tagggaaagg aattccgtat gaactatttt
     1561 gaaacaaatt tagaagaaat atcaagatcc atacagttgc ttgcgcagat atttgaatat
     1621 caggtgtttg aacttcaagt cgaagaaaaa gatggaaatc aaacaggata tgttccatat
     1681 atgatgaacg atgcaattga gtgttatcta agtttccatg gacttcgtat ctctggaaaa
     1741 tatgacaaag actatgaagg agagatgtgg gcacaactgg aagaacgaga aggtcgttat
     1801 gggttgatcg ttcatcaggg agaagaaagt gtttttacaa tgtggtttga tgagatcatg
     1861 gaacatacga actgttaccg gtatcatgag atcggacatt tctggagaga tggagctgag
     1921 cagtggcgcc agctagtcta tatgattgga acgatccgtg agaaatatcg ctttttaggg
     1981 gaagaagtgt gtaatgatca agagatggag atcatgtcac tgatcgaatt tgcaccattt
     2041 tattactatt ttccgatcaa tgaagatcca gaagaatggt atgacaaaac cgaagaagga
     2101 ttagaatgta tgagaaatct tgctatgcag gcaggtgata aagattattt aaaatggatc
     2161 gataaatatg agaagcaccc aaccaaacga atggagatga cgcttgcaaa gaaactgcaa
     2221 gatccaaaga gacaagattt atatgaattg atctgcgaga aagtctgcaa tgcttctgat
     2281 ccatatccag caaggaatta tggagaaaga atcgatcaga agattcaacg atacagggaa
     2341 caggttgata aaaaactgaa agaacaagga tttacaggaa cttatccaca atatgagagt
     2401 gaacatttat cggtttgtgt aacagaagaa catccattta cgatcttgga atctgaagat
     2461 tttaaattta agattcagtt gatgatctca gaatgcagag aaaaacatcc gagaaaaaat
     2521 gctggatttt ttaaaggata tcaaagaaaa ggcaaaataa aaagactcga tttttaatta
     2581 tgaaaatcga gtcttttctt tcactgattt atttattctt cagccgcttc ttcgtcttca
     2641 tcttcaggaa cagcttcagg ttctgcgatg ttaacgacca gagtatcttc tggtgtaaca
     2701 agagagattt tgctgttagc agctaatggc aagtctttga catggatcat atcaccaaca
     2761 ttgtactgtg ttaaatcaat ctcaaccttt tcaacgatat cagctgggaa tgctttgtat
     2821 tcaatttcag ataaggactg ttcaacgata ccattctgta cagcatcttc gtttaataat
     2881 tcgatcgcag cagttgtgtg gatcttttcg tcagcaacta atgcctggaa gttaataaac
     2941 attgtttcgt ttttcatggc attgtagtcg atttctttta cgatggcgtc tacattttct
     3001 ccgtcaacaa gtaaagtaat atgagctcct tttttgttat cctttaagaa tttagcggct
     3061 tcacgggaat caactttcaa tggaatggaa ttttcgatat ctctgccaaa cagatttccg
     3121 ataacataac cttcttttct taatttttta gcctttgttt tcaaatctct tttttctgca
     3181 gttaatgtgt tcataaaaag ttcctccttg aaaaaacatt aagatattat gcatgttatg
     3241 gtataacacc ggaagtactt tgtgtataaa atatataaat acacaaaaag agctcccagt
     3301 gtagtggaag ctcttgctgt attgtaggtg tttctatgca cttgagttta ttatatcact
     3361 actttttaat ttttaaaggg gtaaaattga tgtattttta aaagaacaaa atgaataaaa
     3421 atcacatata ttaaatagaa gaaaaaagaa aggaaaaaag actgtgattt atgattttgc
     3481 cgttatcggc ggtgatatga gacaggtata tttagccaac tatctaaaaa atcaaagata
     3541 tcaggttatc acatatgcaa ccaaaaaaga aggctcagaa tgcaaggaag gtaagtcttt
     3601 tgaaaatgtc ataagagaag cgaagtgcat gattggaccc gtcgtttttt caaaaaacgg
     3661 ggaaatcaca ggaagttcta gtaacaaaaa aatgtcagta gaaacactgt ttcaagaaat
     3721 gacgaaagaa cagattttat ttgggggatg tatcaatgaa gaaattcgac atgaatgtga
     3781 acaaaaagaa atcaaagcat ttgacttcat gaaaatggat gatgtcgcta tctacaatgc
     3841 gatcgcaact gcagaaggtg cgatcatgga agcgatcaag atgcagccga tcaatcttca
     3901 caaaagtcgt tgtctggttc ttggctatgg aagatgtgca aaagttcttg cagcgaaact
     3961 aaaaggaatg gatgctcagg taacagtctg tgctagaaat aaaacagcaa gaagtcttgc
     4021 aaaatctttt ggacttgagg ttatggattt tacggaacta aaacggcgga tctgttagta
     4081 tgatcctatt tttaataccg ttccaaaaca gatcctaaaa gaagatattc tcaggaagat
     4141 ctccaaagaa tcctgtatca tagatattgc ttcttatcct ggtggaacaa accatcaaat
     4201 ggcggaaaaa ctaggaatct gtgcaaaact atgcccaagt cttccaggca tttattcccc
     4261 gaaatcttct gggatcatgc ttgcaaaaaa agttttagaa atcagtggag agatcaaaca
     4321 tggtattgaa aaataaaaga atcggagtag caatgacagg ttccttttgc acttacacaa
     4381 aagcctttga acagatcgat cgcttactag aagaaggtgc gatcctgcag acgatctttt
     4441 ctgacatatc ccagcatatc aaatgcaggt ttggaaaagg tgaggaattt gtaaaaagag
     4501 cacacgatat gacaggaaat gaaccgatga aaacgattga agaagcagaa atcatcggtc
     4561 cgacagggtg tctggatatc ctgattttaa tgccatgtac aggaaatacc atggcgaaac
     4621 ttgccaatgg aattacagat accccggttt taatggcagc taaagcgcag ctgcggaatg
     4681 aaaaaccagt cgttatctct atggcgagca atgatgcctt aggtatgaat cttaagaata
     4741 tcgggaatct tttaaatcat aagcatattt attttgtacc attcggacag gatgatccgg
     4801 taaagaagcc aaactccatg atcgcggata caagactgct cattccgaca attttgcagg
     4861 cattaaaagg cagacaatac cagccggttc tcattgatca taaaaaataa aaagaggtag
     4921 cgtgtatgtg tggaattgca ggatttctaa atcctagaaa caatgataaa aaaacagaga
     4981 aagaaatata tcagatctta aagaaaatga atgaagttca aagacatcga ggtccagatg
     5041 atgaagggat atttttggaa aatggctgtg cattagggca tgtacgattg tcaatccttg
     5101 acctttacag gggacatcag ccaatgtgta aaaagaaaga aggaaatacc tacgcaatca
     5161 tcttaaatgg cgagatttat aacatgaaaa cgttaaaaga acaactgatc aaagaaggag
     5221 aaatgttttc gacaacgagt gacacggaag ttgtattaac cgggtatatc aggtatggaa
     5281 tttcttatat tgaagagttg aatggaattt tttctattgc tctgtgggat gggaaacgga
     5341 agaaattata tttgatcaga gatcgtgttg gtgtgaagcc attattctat acaaaacggg
     5401 gagatacatt aatctttgca tctgagatca aaggcttgtt tgcctatcca ggagtcaaac
     5461 cagtgatcaa cagagaagga ctttgtgaaa tctttgggtt gggacctgca aagtcatatg
     5521 gaaaaggagt gttcaaagat atttatgaag tccttccagg gcatttcctg gaatacgatc
     5581 gtgaagggct gaaagatcgt gcatattggg aattgaaagc gaaagaacac acagacagcg
     5641 aaaaagatac gatcgagcat acaaggtggc tagtaaaaga tgccgtggaa atgcagatgt
     5701 tatccgatat tccaatcagt acatttttat ccggtggagt tgacagtagt cttgtcacag
     5761 caatctgtgc gaaaaagctt caaaaagaag gaaaaaattt aaacacattt tcctttgatt
     5821 ttgtaaataa tcataaatat tttaaatcaa acgccttcca gccaagcgaa gaccgcccat
     5881 gggttgataa gatggttgat tatgcaaaga cagatcacag atatttggaa tgcgacaatg
     5941 aaaatatgat cgaaaatcta tacaaagcag ttgatgccag agatcttcca tgtatggcag
     6001 atgtggagtc ttcgatgcta tatttttgtt cgaaagttgt aaaatataac aaagtaacat
     6061 taacagggga gtgtgcagat gaaatttttg gcggttatcc atggtttcat aaagaggaat
     6121 gcttcaaggc agagattttc ccatggtcta tggatatgca gccaagaaaa atgctgttaa
     6181 atgatgatat catacagaaa gtggatttag aatcttatgc aagaacagct tatcaaaaga
     6241 cgatcaatga gacgccaaaa ctttatggag aagatcagat cgaagctaga agaaggcaga
     6301 tttcttatct gaatctaaga tggtttatgg taactttaat ggatcgcatg gatcgcacga
     6361 gcatgcattg tgggttagaa gcacgagtgc cgtttgctga tcacaggatc gtggaatatc
     6421 tttataatgt tccgtgggag ttaaaatgtt taaatggtgt ggtaaaagga ttgctaagag
     6481 cagcagggga aggaatcctg ccagatgagg tattgtatcg aagaaaaagt ccatatccaa
     6541 agacttatga tccaaactat gaaaaaattt tatccaaaga acttctgaaa gttatggcaa
     6601 aaaaaggagc accgatcaaa gaactgatcg atgcatataa actaagcaga ttcttagaca
     6661 gtccgaaaga ctatggtaaa ccatggtatg gacagctgat ggcaggtcct cagatgatcg
     6721 cttaccttct gcaagtcaat tactggctgg aaaaatacca gatcacattg aatctaagtt
     6781 agctagatta acataaaatg tcttaaaata tatagccatc ccgtaactgt aaatgcacta
     6841 aacatcgttg ttaacatgac aacactggaa gaaagagttc cctcatgtcc catattcttc
     6901 gccataacaa aactacttac tgttgttgcg gaaccaagca tgataaggat ggcaaccaac
     6961 ttatcggtac ggaatccaag tttcactgca attggcagga aaatggcaca aagaccaata
     7021 agtttcataa aagcagcaat ggcagatggt ttgatctttc caaaagcttt tttgatatcg
     7081 aaggtagcgc ccatcgccat gagtcccatt ggagtggcga catttccaat gcttgaaata
     7141 acctttttag ggattgcagg cattggaatt tttaatgcag accagacaag tccagcaaca
     7201 attccgataa ggattgggtt tgtcacgatt cctttgaccg tttttttcac aacagtatgg
     7261 tcaagcccac tctgtccagg tttaaataca gataagacaa caacagccat catattatat
     7321 aatggtacac ttccaatgat catcaaagga gccattccgg catttccata aatgttttgg
     7381 ataaatgcga ttccaagaag agcagcactg cttcggtaag ccgcctgaat aaattctcca
     7441 cggaccgttt tatcacctag cagacaagaa atcagaaaag aaatcgtaat actgatcatg
     7501 gtaacgataa aacaaaacag gacaaactta acatcccaga cttttgaaaa atcaaccgtt
     7561 gcaagatcct caaataccaa gacaggcaaa ggaaccataa aaacaaactt attcattttc
     7621 gaagcaaaaa tatcatcaat ccatccaatc tttttaaaga caagtcctaa gatcatcatc
     7681 aaaaagacag gaactgtagc gtttaagcta aagattaaat tttccataat tactcacttt
     7741 ctgtagtata agctgaactt ttctttggtg tgatcagata gtggtagaca aaatgtttca
     7801 ctgcaacaaa atgttttctc gtccacacaa gacaaaaacc actgaaaaca ggcagaaaac
     7861 atacaagaga accagtatgt ccaaagtttt tgcaagatag ataagaaacg ataacaccaa
     7921 aatggaaaca aagcaaaaca gaagcgaaaa atttccattt tcttaaggcg tcgggatctt
     7981 tactgcaaag atattctgtc atagatgtcg taaactgacg caggttattc gtagaaaaaa
     8041 ttgtagagca ggtaaatcca tccccaccct taaaggaaca ccactgaatt gccattgcaa
     8101 aatacaacgg atagagggca atgaaatcat tcagatccga tggaagaaaa gcaacgatca
     8161 aggctgccat tgcatctata aaaagactga cataaggaag atgcagcctt gtaaacttcg
     8221 gtaagatcac cgtcagtgat aatgcaaaca tataaataaa aacaccaaga agtcgcacaa
     8281 agaaattcgc atccgggtgt ccagcaagat ctgcaaatac agagatcaga ttcgaagtct
     8341 gcgcagaacc aaagagttca tgatgattaa gaatcgcaaa accaccaaga aatcccccaa
     8401 cgattgccat attaaaatgg atccaacgat ccatatccct actacacatg aaaaaaccag
     8461 ctttcttaaa aatatttcca aaatattata tatccatcca gaagaattgt aaagatgagg
     8521 aagagataaa aaagaagcaa aattttcata caacaatgaa aattttatgg tattattact
     8581 gatagagtag cttataaatg caaagataaa aacgcaacgt acaatcattt atgtaccaga
     8641 gccggtaggt agcccggccg tcctatgtaa atagggtaag taacctgccc ctccgggttg
     8701 tccattcttt gcctaattaa ttttaggagg gattcttatg gacaaaacaa atggaaaact
     8761 gacggtatat tttgaaaagc cattttgggt aggcatattt gagcgtacgg aagacggcaa
     8821 actatctgtg gcgaaggtaa cgtttggtgt agaaccaaaa gattatgaag tgcaggaata
     8881 tattcaaaaa cactattttc gtttgaaatt ca
//
LOCUS       DAOMUE010000046         8889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2008_length_8889_cov_2.818542, whole genome shotgun sequence.
ACCESSION   DAOMUE010000046 DAOMUE010000000
VERSION     DAOMUE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2008_length_8889_cov_2.818542"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1148)
                     /locus_tag="PLC71_03585"
                     /pseudo
     CDS             complement(1..1148)
                     /locus_tag="PLC71_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393465.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
     gene            complement(1154..2041)
                     /locus_tag="PLC71_03590"
     CDS             complement(1154..2041)
                     /locus_tag="PLC71_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM01760.1"
                     /translation="MPFDYYRIFYYVAKYKSFSKAAKFLHNNQPNITRCMNIMESELG
                     CTLFIRSHRGIKLTPEGEQLYAHVAIAIQQISSGNDELNRTKNLKSGTVTIGVSETAL
                     HLFLLPKLEKFHKLYPHIKLKISNHSTPQAIKALRKGFVDFSIVTTPLYCEHPLQSQR
                     LCSFRDILIGGPSYRDIGFSPKRLRDLQDFPFISLNEGTATRNFYEKYFYQNQLVFSP
                     DMETATTNQILSLVQHDLGIGFCPEALAKDYLDRGEIIEIPVKEHANPRDICLLTDST
                     RPIGIAGKKLLEILMDSEG"
     gene            2082..2942
                     /locus_tag="PLC71_03595"
     CDS             2082..2942
                     /locus_tag="PLC71_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4866 domain-containing protein"
                     /protein_id="HRM01761.1"
                     /translation="MIYIFYISFFLKYNTVDEKVKKGMISVETIFPREENAEHIFKKI
                     LENNDACERLRELFYEEFANSGDRDLSEKQFVKALFDAYQNRDLSAFLMGICGNSMFD
                     LLRNAFLIPMRFNDKGVTNPVRLTDAEGELIKQTSVNKQISQKQYKMFQQILDQADDI
                     PDYEICLAYGFREKHDYRNKNEINTMKIGEHIGILLLFKLPKEVKEMIEDNEVYSIVW
                     DFMMRLEEQLPRAFMYYGVMDENKFEQQSSEIGIFLPFRHFEHQLEKNIEQANGIGLG
                     CRERILTMIK"
     gene            3159..3521
                     /locus_tag="PLC71_03600"
     CDS             3159..3521
                     /locus_tag="PLC71_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM01762.1"
                     /translation="MGWKIDDGRPIWTQLKEQMIKRIVSGTYSSGEKLPSVRDLAGEA
                     GVNPNTMQRALSALDQDGLTITNRTSGRCVTEDVTKIEECRKDIAHKIVKQYIRDLEE
                     LGYNKEDAVALIGKEEEE"
     gene            3518..4252
                     /locus_tag="PLC71_03605"
     CDS             3518..4252
                     /locus_tag="PLC71_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM01763.1"
                     /translation="MKDLNLIELTDLEKRYGKKEALTGINLTIGRGKIIGLLGPNGSG
                     KTTLIKLLNGLLQPTSGEIKVNGKAPGVDSKKIISYLPDKMYFADWMKIADLLDFFED
                     FYEDFDRKKAEGMCETLKINTEAKIKSLSKGNKEKVQLVLVMCRKAQLYLLDEPIAGV
                     DPAARDFILDTILNNYNEEGTVIISTHLIADIEKIMDEVIFIKEGNIVTYKSADDLRE
                     ESGKSIDALFREIFHTDVYRGGDEVC"
     gene            4246..5007
                     /locus_tag="PLC71_03610"
     CDS             4246..5007
                     /locus_tag="PLC71_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01764.1"
                     /translation="MLGKLIKYDLKALAKILAPLWGVLLVMGLIFGISIRSNLDGIGN
                     TMIVFSLVVIVAVIVAIFVMNVIIVIQRFWNGLLQEEGYLMFTLPVTTRSLILSKVIS
                     ALIISCGTALVISLLGVEIIAISPVKLMDTVTYFGNWVVKVHAGPWIGYGAVIAVVGL
                     LSGIYHIYAAMVIGQLSNGNRFLFAFVAYAVLSIIVSLIGIPSMTNLDNMGSSLQNTF
                     GFDSDLWIYLVENIVIIIIYHIVTEVILTKKLNLE"
     gene            5043..5795
                     /locus_tag="PLC71_03615"
     CDS             5043..5795
                     /locus_tag="PLC71_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01765.1"
                     /translation="MLRKLMKYDLKYYRKIMLPLWAVLIIYGIVHGINTVFNYETFYD
                     HIPIKAQAIVVVTGIYFIFCVNIILVIQRFWKSIYGKESYLTHTLPVTVRKLILSKVF
                     SAIIISFTTVFIFFAENYILIYIQSIVYHGEASLQGIPIRHFQDFTKPKVALTILYMI
                     VYGIIWTMHLIYQAYTAICIGQMRSKNRFATTIIVGAFLFLAGYEMQEILLRHNRYYS
                     WFYLPCLIAFLVEIVLCHLVIEFILTRKLNIN"
     gene            6317..7957
                     /locus_tag="PLC71_03620"
     CDS             6317..7957
                     /locus_tag="PLC71_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRM01766.1"
                     /translation="MSSTNILGLLGGLALFLYGMQMMSNGLESVAGNKMKDILEKLTS
                     NRFLGIIVGALITAVIQSSSATTVMVVGFVNSGMMTLNQAVWIIMGANIGTTITGQLI
                     ALDVGALAPLIAFIGVAIVVFSKNEKVQFVGEIIAGLGILFVGMNMMGDSMIPLREYP
                     PFINLMTRFSNPLIGIIAGMIFTAVIQSSSASVGILQALALSGVISFHDAAFVLFGQN
                     IGTCITAILASIGTERSAKRATIIHLSFNIIGTAIFTILCLVTPLTNYVAGFSGSSIT
                     KQIANMHTLFNISTTILLIPFGNYLAKLATKILPEKAEEKEHHKHLKYIKSVDTRVDA
                     TFGTSAINLQNLRNEIQRMLEMARVNVEESFDAFLAGSSRFLGDVDKREDYIDYLNKE
                     ISRAAAKMMAHETNVKASKNIGSYLDMTSNIERIGDHAVNICDYTKLIEEKHIVFSDD
                     AKQQMEEMKMICEKMFHNISKVPEDTQEWLQKVHDSENFIDQKTEEFYESHLERINRG
                     ECNDEACIIFSEMLTDFERIGDHTWNVAKQMAKIIERG"
     gene            8101..8757
                     /gene="rpe"
                     /locus_tag="PLC71_03625"
     CDS             8101..8757
                     /gene="rpe"
                     /locus_tag="PLC71_03625"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRM01767.1"
                     /translation="MDYILSPSILSADFANLGADVKEAVKAGAKWVHIDVMDGMFVPN
                     ISLGLPVIKSLRKETEALFDVHLMIEEPGRYIDDFAEAGADLITLHAEATKHLHRAIQ
                     QVKAKGLKVGVALNPATPLSALDYVLEELDMVLIMTVNPGYGGQSYIAKMDDKIKELR
                     AIINEKGLDIDIEVDGGISAKNVAHVKECGANVFVAGSAVFNGDIASSAKDILEVMEA
                     "
     gene            8760..>8889
                     /locus_tag="PLC71_03630"
     CDS             8760..>8889
                     /locus_tag="PLC71_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM01768.1"
                     /translation="MSECSHDCSSCSANCGSRTQEKTDFLEPLYRESKVKKVIGIVS"
BASE COUNT         3084 a         1401 c         1877 g         2527 t
ORIGIN      
        1 gatcgatggc acagtcagtg agattcgagg ataaccaatc gaagttttga tcatagatgc
       61 tagaagacca gcaagcaaag ctccaaggaa tgcagccgca gctggcggaa atgatgcaag
      121 atcaaccagt tccagacgag ttgtatttgc aattgccccg atgatccctg ctgtagcagc
      181 aagttgtaat ggactgttaa acattacgga gaaacctaaa actccaaaaa agcttgcgat
      241 cagtcgaaat atcaggtatt ctgacttgct taaattcaat ggaagaaaat ccatcggttt
      301 taaatgaaga actaaagcaa gcatccatgc agctaaagtt gcaacagcaa tgataatcag
      361 tgcgtaagcc agacgttcaa gtccggaacg catatccaat tttgcaaggt caattccact
      421 tgtgataaat ggaaatcctg gaataatgaa taacatagaa cagatataac cagcttgatg
      481 gtgtatcgaa atatgaaatc cagatttcaa tgccatgaag gcaattgtgt atacaataca
      541 tgccaaagca acgcttgccg cagtgcatag gaataaagtt aaatgatgtt gattcatttt
      601 tgcccggaga aatttccggc accagcacct aagaatgcac aaaacatctc aatcggtccg
      661 ccaccgagta aaaaagtaaa tgcactgcat gcaacagctg cagccagacc aaggaatggc
      721 ggagagtaaa atccatgaat ttcatgaatc tgatcaagta gtgtatgaag ttcatctcca
      781 gacatcttct tgcagttttt ggaaaatttg ttaacaaaca attccattcg ataaagtttt
      841 gatgtattta cgcctgtatt aaacagacat agggattctg tataacagtt accaccatca
      901 atacaagtat attcaattga taataaacca atatcagcag tacaagtaat tccaagttct
      961 ctagccagtg tgttcataga acttcgtact ctccatgctc cagttccgca agatagaagc
     1021 ataattccag tacgaccgat gatcgaggct ttctcactta acgaagcttc cgtgatcgga
     1081 cgaccaatac tttttcttgt ataatcatgc caaggaatcc gcatatgatt atgaaattct
     1141 tcctccatat atatcacccc tccgaatcca taagtatttc taataatttt ttcccagcaa
     1201 ttccaattgg tcgggtagaa tcggtaagga gacaaatgtc tcgtggatta gcatgttcct
     1261 ttacaggaat ttctataatt tcaccacgat caagatagtc cttagcaaga gcttccggac
     1321 agaaaccaat tccaagatca tgctgcacca gagataagat ctggttcgtt gtagcagttt
     1381 ccatatccgg agaaaaaact aactgattct gatagaaata tttctcataa aaatttctgg
     1441 ttgctgttcc ttcatttaat gaaataaaag gaaaatcctg cagatcacgt agtcttttcg
     1501 gtgaaaaacc aatatcccta taagaaggtc cgccaattaa aatgtcacgg aaagagcaaa
     1561 gtcgttgcga ttgtagtgga tgttcgcaat acaatggtgt tgtcacaata ctaaaatcaa
     1621 cgaatccttt tctcaaagct ttgattgcct gtggtgtgga atggttggaa atttttaatt
     1681 taatatgtgg ataaagtttg tgaaacttct ctaatttcgg cagcaaaaat aaatgaagtg
     1741 cagtttcgct tactccgatc gtaacagttc cagatttcag attttttgtt cgatttagtt
     1801 catcatttcc agaagaaatc tgctggatcg caattgctac atgggcataa agttgttccc
     1861 cttctggcgt tagcttgatt cccctgtgtg accggataaa taaagtgcag ccaagttcac
     1921 tttccataat attcatgcag cgagtgatat ttggctgatt attatgtaag aatttcgcag
     1981 ctttagagaa actcttatat tttgcaacat aataaaagat acgataatag tcaaaaggca
     2041 tgattgacct cctagttata taaaatttgt atgttattga tatgatatat atattttaca
     2101 tatcattctt cctaaagtat aatacagttg atgaaaaagt caagaaaggg atgatcagcg
     2161 tggaaacaat ttttccaaga gaagaaaatg cagagcatat atttaaaaaa attctggaaa
     2221 ataatgatgc ctgtgaacga ttgagagagt tattttatga agagtttgcc aacagtggag
     2281 atcgtgatct ttcggaaaaa cagtttgtga aagcactatt tgatgcatat caaaataggg
     2341 atctttccgc tttcttgatg ggaatctgtg gaaacagtat gtttgatctg ttaagaaatg
     2401 cgtttttgat tcctatgaga ttcaatgata aaggtgtaac aaatcctgta agattaacag
     2461 atgcagaggg cgagctgatc aaacaaacat ccgtaaacaa acagatttca caaaaacaat
     2521 ataaaatgtt tcagcagatt ttagatcagg cggacgatat accggattat gaaatctgtc
     2581 ttgcttatgg atttcgtgaa aaacacgatt atcggaacaa aaatgaaatt aacacaatga
     2641 agattgggga acatattgga atcctgctat tatttaaact acctaaagaa gtaaaagaaa
     2701 tgatagagga taacgaagtc tacagcattg tttgggattt tatgatgaga ctggaagaac
     2761 aattaccaag agcgttcatg tattacggtg taatggatga aaacaaattt gaacaacaaa
     2821 gcagcgagat tggcatcttt cttcccttcc gtcactttga acaccaacta gagaaaaaca
     2881 tcgaacaggc caatggtatt ggtctgggat gtagagaacg gatcctgaca atgatcaaat
     2941 aaaaaggaac tattttattt atagttacca atagtagtca taaaagacat cgcatgaaat
     3001 acaaatattc gtgcgatgtc ttttgtgttt tctatcaatt tatgatgaga gtgtcaaaag
     3061 tataaatgaa ataaggattg acattttata aaaagtagca tataatatta ttgtattaag
     3121 gaagtaatac aataatacaa taagaaggaa ggagaactat ggggtggaag atagatgatg
     3181 gaagaccaat ctggacacaa ctgaaggaac agatgatcaa gaggattgtg tcaggaacct
     3241 attcaagcgg agagaaacta ccaagtgtca gggatcttgc aggagaagca ggggtgaatc
     3301 cgaatacgat gcagagagcg ttatcagcac ttgatcagga tggattaacg atcaccaacc
     3361 gaacaagcgg gcgctgtgtc acagaagatg tgacgaagat agaagaatgt cggaaagata
     3421 tagcacacaa gatcgtaaaa caatacatca gggatttaga ggaacttgga tacaataagg
     3481 aagatgcagt cgcattgatc ggaaaggaag aggaagaatg aaggatttga atttaataga
     3541 attaactgat ttagagaaac ggtatggaaa gaaggaagct ttaacgggaa tcaacttaac
     3601 aatagggaga ggaaagatca tcgggctttt aggacccaat ggttctggaa agacaacatt
     3661 gatcaagtta ttaaacggtc tgttgcagcc aacatcaggt gagattaaag tcaatggtaa
     3721 agcacctggt gttgacagca agaagatcat cagctatctg ccagataaga tgtattttgc
     3781 agattggatg aagattgcag atctgctgga tttttttgaa gatttttacg aagattttga
     3841 ccgtaaaaaa gcagaaggca tgtgtgagac attaaagatc aacaccgaag cgaagatcaa
     3901 aagcttatcc aaaggaaata aagagaaagt ccagttagtt ttggtaatgt gccgaaaagc
     3961 acaactctat ctgctagacg aaccgatcgc aggagtcgat cccgcagcaa gggattttat
     4021 cttagatacg atcttaaaca attataatga ggaaggaacc gtgatcattt caacacatct
     4081 gatcgcagat atcgagaaga tcatggatga ggttattttt atcaaagaag gaaatattgt
     4141 aacatacaag tcagctgatg acttaagaga agaaagcggc aaatccatcg atgccctgtt
     4201 ccgtgagata tttcatacag atgtatacag aggaggggac gaagtatgct aggaaaatta
     4261 ataaagtatg atctgaaagc attagcgaag attttagcac cattatgggg tgtactctta
     4321 gtgatgggac tgatctttgg aatcagtatt cggtcgaatc ttgacggaat tggaaatacg
     4381 atgatcgtat ttagtcttgt ggtcattgtt gcagtgatcg ttgcgatctt tgtaatgaat
     4441 gtgatcattg tgattcagcg cttctggaat gggttattgc aggaagaagg atatttaatg
     4501 ttcacgcttc cagttactac aagaagcctg atcttatcca aagtgatcag tgcattgatc
     4561 atcagctgtg ggacagcact cgtaatctct ttattgggag tagagatcat tgcgataagc
     4621 ccggtaaaac ttatggacac cgtaacatat tttggaaact gggtcgtcaa agtacatgca
     4681 ggaccatgga ttggatatgg agcggtgatc gcagtggtag gtttgctaag tggaatctac
     4741 catatatatg cagcaatggt gatcggacag ttgagtaatg gcaatcgatt cttgtttgca
     4801 tttgtggcat atgcagtgtt atcgatcatt gtatcactga tcggaattcc aagtatgaca
     4861 aacttagata atatgggatc aagtctgcag aacacatttg gatttgatag tgatctgtgg
     4921 atctatcttg tagagaatat tgtgatcatt atcatttatc atattgttac agaagtgata
     4981 ttaacgaaga aattgaatct ggagtaggaa aaataggagg gataaggaga ggaggaaaaa
     5041 ttatgttaag gaaattgatg aaatatgatc taaaatatta ccgtaagatc atgctgccac
     5101 tatgggctgt tttgatcatt tatggaattg tccatggaat caatactgtg ttcaattatg
     5161 aaacatttta tgatcatatc ccaataaagg cacaggctat tgttgttgtg accggtattt
     5221 attttatatt ctgcgtgaat attattcttg tgatacaaag gttctggaaa agcatatatg
     5281 gaaaggaaag ctaccttaca catacacttc cagtgactgt gagaaagttg atcttgtcaa
     5341 aggttttcag tgcgatcatc atcagtttta caacagtttt tatctttttt gcagaaaatt
     5401 atatattgat ttatatacaa agtattgtat atcacggaga agcttcactt caaggaatac
     5461 cgattcgaca ttttcaggat tttacgaaac caaaagttgc tttaacaatt ctttatatga
     5521 tcgtatatgg aatcatatgg acgatgcatt tgatctatca ggcatataca gcaatatgca
     5581 ttggacaaat gagaagtaaa aacagatttg cgacaacgat cattgttgga gcttttttat
     5641 tcctggctgg ctatgaaatg caagagatat tgttacgaca taatagatat tacagttggt
     5701 tttatcttcc ttgtctgatt gcttttcttg tggaaattgt gttgtgccat ttggtgattg
     5761 agtttatttt gactagaaag ttaaatataa attagataat agaattgaca taaaaaatta
     5821 tagatgttaa tataacaata taaaaaggtg ctaccgatga cggtttgcct gattaatata
     5881 agttttatag accgcttact ttctcagggt agggcggtct ttttctgtct tggcatcatg
     5941 ctttccacgc acatagccaa gactataaaa agtcgcacac agtgagatta ctgcgattag
     6001 gtctgtaatg gttagcataa tttcaagtcc cctttccgtc agatttctct atgaaagaga
     6061 tggatctatg taatcggggc tagaactccc cgtaggaagc aaaccgccat tcacgtcaat
     6121 gtgaagtaac accttgacgt ttattttaac agaaaacata tattaattca ataaaaaaca
     6181 aagtataaac atataaaagt acaaaaaatt ttacatacat ttaacacaac acatctccta
     6241 aataagttaa aataaaactg ttgtaaaaaa taagtgaaag aaaagtaaag aaaagaacaa
     6301 ctaaaggaga aaaaaagtga gcagtacaaa cattttaggt ctgttgggag gattagcatt
     6361 attcctatat ggtatgcaga tgatgagtaa cggattagaa tccgttgccg gaaataaaat
     6421 gaaagacatt ttggaaaaat tgacatccaa tagattctta ggtattatcg taggagcttt
     6481 gatcacagcc gtgatccagt catcctcagc tacaacggta atggtcgtag gatttgtaaa
     6541 ctctggaatg atgacattaa atcaggccgt atggatcatc atgggagcta atattggtac
     6601 aacaattaca ggacagttaa ttgccttaga tgttggggca ttagcaccat taattgcgtt
     6661 tatcggtgta gcaatcgttg tattttcaaa gaatgaaaaa gtacagtttg tcggagaaat
     6721 cattgcagga cttggtattt tatttgttgg aatgaacatg atgggagatt ccatgatacc
     6781 acttcgtgag tatccaccat ttatcaacct gatgacaaga ttctcaaatc cattgatcgg
     6841 aatcatcgca ggtatgatct ttacagcggt catccagtca tcttcagctt ctgtaggtat
     6901 cctgcaggca ttagcattaa gcggagtgat cagtttccat gatgcagcct ttgtattatt
     6961 tggacagaat atcggtacat gtattacagc gatcttagca tcgatcggaa cagagagaag
     7021 tgcgaagaga gcaacgatca ttcatctaag cttcaatatt atcggaacag ccatttttac
     7081 aattttatgt ttagtgacac ctttgacaaa ttatgtggca ggattcagtg gaagcagtat
     7141 tacaaaacag attgccaata tgcatacatt atttaatatc agtacaacca tcctgctgat
     7201 cccatttgga aattatctgg caaaacttgc aaccaagatc cttccagaga aagcagaaga
     7261 gaaagaacat cacaaacatt taaaatacat caaatcagtt gatacaagag tggatgcaac
     7321 atttggtaca tcagcgatca acttacagaa cttaagaaat gaaattcaga gaatgcttga
     7381 gatggcaaga gtcaatgtag aagaaagttt tgatgcattt ttggcaggaa gcagcagatt
     7441 cttaggtgat gttgataaaa gagaagatta tattgattat ctgaacaaag aaatttcaag
     7501 agccgcagca aagatgatgg cgcatgaaac gaatgttaaa gcttcaaaaa atatcggttc
     7561 atatcttgat atgacaagta atatcgaacg aattggagat catgcagtta atatttgtga
     7621 ttatacaaag ttgatcgaag aaaaacatat tgtattttca gatgacgcaa aacagcagat
     7681 ggaagaaatg aaaatgatct gtgaaaaaat gttccataac atttctaagg taccagagga
     7741 tacgcaggaa tggcttcaga aagttcatga ttctgagaac tttatcgatc agaagacaga
     7801 agaattctac gaaagtcact tggaacgtat caacagagga gaatgtaatg atgaagcatg
     7861 catcattttc tctgaaatgt taacagactt tgagcgtatc ggtgatcata catggaatgt
     7921 tgcgaaacag atggcaaaga tcattgaacg tgggtaaaac aattacataa aattcaaaaa
     7981 gaaccttctg aaagaactaa aataagaagg ttcttttttt gatagtaact agacttgtta
     8041 taaacaatac gttatactaa aaagagtgct aaaaacaggt gtgtaaagga gaaatgagtt
     8101 atggattata ttttatcccc atcaattttg tcagcagatt ttgcgaatct tggagcagat
     8161 gtaaaagaag cagtaaaagc aggtgcaaaa tgggtgcata ttgatgttat ggatggaatg
     8221 ttcgtaccta atatttcctt gggacttcca gtgattaaat cacttcgtaa agagacagaa
     8281 gctttatttg atgtacattt aatgattgaa gaaccaggaa gatatattga tgattttgcg
     8341 gaagcaggag ctgatctgat cactcttcac gcagaagcaa caaaacatct tcatagagcg
     8401 atccagcagg taaaagcgaa aggattaaaa gttggagtag ccttgaatcc agcaacgcca
     8461 ttatctgcat tagattatgt attagaagaa ttagatatgg tactgatcat gactgtaaac
     8521 ccaggttatg gcggacagtc ttatattgcg aagatggatg acaagatcaa agaattacga
     8581 gcgatcatca atgaaaaagg actggatatc gatattgaag tagatggagg aatctcagca
     8641 aagaacgtag cacatgtcaa agaatgtgga gcaaacgtat ttgtagcagg atccgctgta
     8701 tttaacggtg atattgcatc tagtgcaaaa gatattttag aagttatgga ggcgtaaaaa
     8761 tgagtgaatg tagtcatgac tgcagcagtt gtagtgcaaa ctgcggaagc agaacacagg
     8821 agaaaacaga ttttcttgaa ccattatata gagaaagtaa agtaaagaag gtcatcggta
     8881 tcgtcagtg
//
LOCUS       DAOMUE010000047         8812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2033_length_8812_cov_2.798675, whole genome shotgun sequence.
ACCESSION   DAOMUE010000047 DAOMUE010000000
VERSION     DAOMUE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2033_length_8812_cov_2.798675"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="PLC71_03635"
     CDS             complement(<1..1389)
                     /locus_tag="PLC71_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HRM01769.1"
                     /translation="MKTICKKISVSILTLLMIMTMYLSGSGESLFLKNRDVKAFTGKA
                     KLVMSQALSSNKISEIDRPASEGLWSMTADGHRVFCLNSGKTMCSGDTLKYKTINAAT
                     YEKKGIAKALNWYFRSSGKNTKDLSLCQAYIWACGHGANKQNTVYQAGKNVDRGYSQK
                     DAKKFCKMISDQDPEGTIYYYTVKKCVKKKKLDSHQVLFGFRHTPPDIEKAKTSATKS
                     GTSPDNVRIKIKKKDAETRAGLSGAVFQIYMDGNYQGSVTTDDNGEASYTVQRTVSYS
                     VTSTKKTYVKNWNDLSKSQQKEATDNGWYDSSAKAYAVAMQEAQKLAEQKISALKSAS
                     VHTWMVRETKSPFGHLIPDQTDQSKVEQGGVRSFTFNYTNEFQKSDLEIFKQGTVKNK
                     RGDLGVEANLQNAVYELYADHDITGSDNKSVVYKAGTLVTQMVTDADGYAKVTDLYPG
                     YYRLHEKYAPLGY"
     gene            complement(1415..2932)
                     /locus_tag="PLC71_03640"
     CDS             complement(1415..2932)
                     /locus_tag="PLC71_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HRM01770.1"
                     /translation="MKNAKKILERKRKIIVVLVVMVSFVSVLAWYQMKKAHAYQSYNT
                     GGNKHFKDGHLTVNINGPNGKSDSLTMYIHSDEYEKNKNVSGGTFNNSRHPYTVTTYS
                     GGGGDYKLTVNKQTIYSEKNSNNNYTLLTIKVWYLLPAHEQHRSWEWVTVDKPVFSDP
                     SKDITLYCGGNDGFWENSYHDTQEHWKSVNIHVSTGLVGVASADRDGWKTYDWCSINL
                     NLEKPSRSIYYNGNGGDGADVTWNFTDGDYFSFPTVSRRGYTLEGWLDEGTKWMSNTG
                     WLVCGNYTETAQWTANTYTVNYDGNGEDSGYVNDGTATYDQNYSFAENGYTKKGYAFV
                     GWNTDKHAVRARYSPGETITWSNTEGITLYAVWSKNSYEVTFDGNGASGSKKTVELKY
                     GQDDILPANTFERPGYTFLGWSEDPNAIKAKYTDRQAVNTLCDAGQTCELYAVWKKTD
                     GSFDTHNIIHDDGMFNGSIELEGQNGTGFSRDHVDSEYGRIDKEGQPGYFTNRYK"
     gene            complement(2956..3663)
                     /locus_tag="PLC71_03645"
     CDS             complement(2956..3663)
                     /locus_tag="PLC71_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HRM01771.1"
                     /translation="MKQKIINIVGIFFLVIGITLLLYPEIISYLKQKQSNQTVKELTQ
                     RRSKRKQDDLLYQKAVCYNRKIFKEKQAGLKDVFSYRSAPIVLRNEKNTFGYIKIPKM
                     KQKLPLYLGATMENMRKGAAIMGQTSLPVGQKDSNCVIAAHRGYRGIPYFRDIEQLKT
                     GDQVIIRNLWERLDYRVTKIKVIDPYDMDKILIQKGKDMVTLLTCHPYRGHGRYRYVV
                     YCMRNHGQKIRKQKEDR"
     gene            complement(3660..4514)
                     /locus_tag="PLC71_03650"
     CDS             complement(3660..4514)
                     /locus_tag="PLC71_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HRM01772.1"
                     /translation="MKQKIIPILIVLTGFLLLFYPFTSNYLFEKSAGSTVESYQEKAA
                     GMDQAIIKKVMDEAKQYNGELMRSSVQLTDPFKEKRLDGETVHYNRILNIDGSSIMGY
                     LKIPCISINLPIYHGTSGTVLEHGIGHLATSSFPIGGKDTHAVLTGHTGLSSAKIFTD
                     LTEMKKGDFFFIHVLDKKLAYRVDQITVVEPQDTKELQIMEGKDHVTLVTCTPYGVND
                     KRLLVRGVRTAYHAKEEEIRARNHHSQWMEVYKRAIFAGLLIICVLIAARKVYEKMKR
                     KSGERRYG"
     gene            complement(4516..6474)
                     /locus_tag="PLC71_03655"
     CDS             complement(4516..6474)
                     /locus_tag="PLC71_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HRM01773.1"
                     /translation="MKIQNMIKKIMIAVLSAAMMLAPIVNIKAASTDVVDTSKTGSIT
                     IHKYDMTAAKQAGVNTSQFTPTGKQDAAAEAALEKYAIKGVEFSYLRVGDVEQQSENG
                     KIQMIYELPTTIQQILGLTSSDAAKTEGSKTYFTSQQINEKLAKALEDNTVTKDKLED
                     YMGKNGTAMDETNANGVTSKDKLPLGLYLIVETKAPENVTYTTNPWFVQLPSTDSKGD
                     DWFYDVICYPKNETGNPTLDKRVRNNPDQDNVTTANTDRLADFTSARNEYKYQSTVTA
                     SKAERLDYQFISKLPHITSSTTYLSTYTFNDTMAKGMTYGKDAVIAIYETKDAADRTN
                     VNNVEKSGALAVWKSSDTDPKFAATYGKSGDDPAMKIEMTKAGLNELNKKYSDKYIVV
                     YYTAKVNTDDSVILGDKGNPNDVSLTWKRTNTNYYDILKDKCIVYSYGYDLTKKFSDS
                     KGDATKVKFVVKNKTDNYYLVARADRAGVYQVTGKSAKEADATQFSPSSDGKLVINGI
                     EGDEYGFTETHSDAGYSLLKKEVIVKVKETKADITPTEANITGIQSKSDADSTANDGV
                     PNGAVLKNDVTVQTTAASATVDEIKATMTKHDDSGNAYINMQITNQKQFMLPMTGGAG
                     NYLLIIAGVVVAGCGILILNKNRRRSQR"
     gene            complement(6570..7865)
                     /locus_tag="PLC71_03660"
     CDS             complement(6570..7865)
                     /locus_tag="PLC71_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRM01774.1"
                     /translation="MRKTRKTAAVVALYTFLMLGSVSVVRADQPVTREQISSEQTETK
                     TDSSAQTTESEATTETTTQTTQAKPTTEATTHTHTTAAATHTKKHTATTHTKKHTAVT
                     HTKKQKTKSDQDKKKDKKDKKKDKKDKKSIIPKVYNDHSLEMKDKKETIQGFIYFNQA
                     DAAWNDNGYQIKSSGCGPSAMAVCISSLTNKWVTPVDTTVWAYKNGYYSSAGASHEMV
                     PALAQQYKLECHGLGSDVSKVRDALKKKHPVVAMMGPGYFTKKGHFIVLVAIDDNDQV
                     TVADVGSRQRTQYKYPLKEVIAQTKSASAGGPCWEIYSDQKISAKADKKAKQLKAEKK
                     YRSKEFKAMYNEIKSVLQKNYQLAVPLKKGTLVSEEQFVTITSLGINDKVSVMDASKK
                     LTSDVDLDTVVDEIQAEAVKDSFWNTIMIAPDKTNLTDK"
     gene            complement(7973..>8812)
                     /locus_tag="PLC71_03665"
     CDS             complement(7973..>8812)
                     /locus_tag="PLC71_03665"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM01775.1"
                     /translation="RNSMSKIKLVAMDLDNTLLDSEKQISKHTEEVLKEAIRRGVYIV
                     PATGRIFKAIPEFLRDIPGVRYAVCCNGATVYDQKEDKIIYTNHLPKETVFSLFDILE
                     KYHCTRDIYQNGQGYMERCYFDHLAEYGVSDHVLKLVRDTRLPLDDLRKYIEEHPLGI
                     EKINVFFDDMEERETARQELIEKNIASVSSSLGNNIEINQIGCDKGDGLLHLAEQIGL
                     SMDEVMACGDAGNDTMMIKAVGTGVVMENGQPDLKEIADFVTKTNNEDGVAYAIEKLV
                     FEA"
BASE COUNT         2102 a         2083 c         1402 g         3225 t
ORIGIN      
        1 atatcccaac ggagcatact tctcatgtaa acggtaatat cccggataca gatctgtcac
       61 ttttgcataa ccatccgcat cagttaccat ctgtgtcaca agtgttcctg ctttatagac
      121 aacactcttg ttatcggagc ctgtgatatc atggtcagca tatagctcat agacagcatt
      181 ctgcaagttt gcttcaactc ctagatcccc tcgtttattc tttacagttc cctgtttaaa
      241 gatctccaga tcactctttt gaaattcatt ggtatagtta aatgtgaatg atctgactcc
      301 accctgttcc actttgctct gatctgtctg atctgggatc aggtgtccga atggggattt
      361 tgtttccctg accatccaag tgtggaccga ggcactcttt aaggctgaaa tcttctgctc
      421 tgcaagtttt tgtgcttcct gcattgccac agcatatgct tttgccgaag aatcgtacca
      481 gccattatct gtcgcttctt tttgctgact ttttgaaaga tcgttccaat tctttacata
      541 agtctttttc gttgatgtca cactataact taccgttctt tgaacagtat aagaagcttc
      601 tccattgtca tcggttgtta cacttccttg ataattccca tccatataga tctgaaatac
      661 tgctccagaa agccctgctc ttgtttccgc atctttcttt ttgatcttga tccttacatt
      721 atctgggctg gttcctgact ttgttgcact cgttttcgct ttttcaatat ctggcggtgt
      781 atgacgaaat ccgaacaaaa cctgatggga atccagtttc ttctttttca cacatttctt
      841 cactgtataa tagtagatcg ttccttctgg atcctgatca ctgatcattt tacagaactt
      901 ttttgcatct ttctgcgaat aacctctgtc cacatttttt cctgcctgat aaacggtatt
      961 ttgcttattc gctccatgtc cacatgccca gatgtatgcc tgacacaatg acagatcttt
     1021 tgtattcttg ccgcttgacc tgaaatacca gtttaatgct tttgcaattc cctttttttc
     1081 ataagttgct gcattgatcg tcttgtattt taaggtatct ccagaacaca tggtctttcc
     1141 agagtttaag caaaacactc tgtgtccgtc agctgtcata ctccacagac cttctgatgc
     1201 aggacgatcg atctctgaga tcttattact tgataatgcc tggctcatca ccagtttggc
     1261 ttttcctgtg aaagccttga cgtctctatt cttcaggaac agtgattctc ctgaaccact
     1321 cagatacatt gtcatgatca tcaggagggt aagaatactt accgatatct ttttacagat
     1381 cgtcttcatt tgtttcaaac tttcttcctc ctttttactt gtaacggttt gtgaaatatc
     1441 ctggctgccc ttccttgtcg atccttccat actcagaatc tacgtggtct ctcgaaaatc
     1501 cagttccatt ctgcccttcc agttcgatcg agccattaaa catgccgtca tcatgaataa
     1561 tgttgtgtgt gtcgaatgat ccatctgttt tcttccagac agcatacaat tcacaagtct
     1621 gtccagcatc gcagagagta ttgactgcct gtctgtcggt atattttgct ttgatcgcat
     1681 tcggatcttc actccatcca aggaaggtat atcccggccg ttcaaatgta tttgctggca
     1741 ggatatcatc ctgtccatat tttaattcca cagtcttttt gctgcctgat gcaccatttc
     1801 catcaaatgt tacttcataa gagttcttgg accaaacagc ataaagtgtt ataccctccg
     1861 tgttactcca tgtgatcgtc tctcctggag aataccgtgc tctcaccgca tgtttatctg
     1921 tattccatcc aacaaaggca tagccttttt tcgtgtatcc attctcagca aagctgtaat
     1981 tttgatcgta cgtggctgtc ccatcattca catatccaga atcttctcca tttccatcat
     2041 agtttacagt gtatgtattc gctgtccact gggctgtctc cgtataatta ccacatacca
     2101 gccagcctgt attggacatc cattttgttc cttcatccag ccatccttcc aaggtataac
     2161 ctcttcttga taccgttgga aacgaaaaat aatctccatc cgtaaaattc catgttacat
     2221 ctgcaccatc tccaccattt ccgttgtaat aaatgctacg gcttggcttt tccagattca
     2281 gatttataga acaccagtca taagttttcc atccatctct gtcagcggaa gccactccga
     2341 ccaatccagt actgacatgg atatttactg atttccaatg ttcttgagta tcatgatagg
     2401 aattttccca aaatccatca tttcctccgc aataaagcgt tatatccttg ctcggatctg
     2461 aaaatactgg tttatccacc gttacccatt cccaggatcg atgctgttca tgggccggta
     2521 acagatacca gactttgatc gttaaaagtg tatagttgtt gttactgttc ttctctgaat
     2581 atatcgtctg tttattcact gtaagcttat aatctccgcc tccaccggaa tacgtcgtta
     2641 ccgtatatgg atgtctggaa ttattaaatg tcccaccgct gacattttta tttttttcat
     2701 attcatcact atgaatatac attgtcagac tatcgctttt cccatttggt ccattgatgt
     2761 tcactgttaa atgtccatct ttaaaatgtt tgtttcctcc tgtattatat gattgataag
     2821 catgtgcctt cttcatctga taccatgcca gaacagaaac aaatgaaacc atgaccacta
     2881 ataccactat gatctttcgt tttctttcca ggatcttttt tgcattcttc atcacatcct
     2941 cctctccatc tatttttatc tatcttcctt ttgtttcctg atcttctgtc catgattcct
     3001 catacagtac accacatacc gatatcttcc atgtcctcgg tacggatgac aggttaaaag
     3061 tgtgaccata tctttccctt tttgaatcag aattttgtcc atatcatatg gatcgatcac
     3121 tttaatcttt gtaacccgat aatccagtct ttcccataga ttcctaatga tcacctgatc
     3181 gcctgttttt aactgttcga tgtcccgaaa atacggaatt ccccgatatc cccgatgtgc
     3241 agcgatcacg cagttgctgt ctttttgtcc tactggcagg gatgtctgtc ccatgatcgc
     3301 tgctcctttt ctcatatttt ccatcgtcgc tcccaaatat aatggaagtt tctgtttcat
     3361 cttcggtatc ttaatgtaac cgaaggtatt cttttcgttt cttaagacga tcggtgcact
     3421 gcggtaactg aatacatcct ttagtcctgc ttgtttttct ttgaatatct tcctgttata
     3481 gcagactgct ttttgataca gtagatcatc ctgttttctt ttgcttctcc tctgcgtcaa
     3541 ttctttcacc gtctgattgg actgtttttg tttcagatat gatatgatct ccggataaag
     3601 cagtaacgtg atccctatca ccagaaaaaa gattccaaca atatttatga tcttttgttt
     3661 cacccatatc tcctttctcc gctttttctt ttcatctttt catatacttt tctggctgcg
     3721 atcaatacac aaatgatcaa tagtcctgca aagatggccc gtttgtaaac ttccatccat
     3781 tgcgaatgat gatttcttgc tcttatctct tcctcttttg catgataagc tgtgcgaact
     3841 cctcggacta ataaacgctt gtcattaact ccatagggtg tgcaagtaac caatgttaca
     3901 tgatcttttc cttccatgat ctgaagttcc tttgtatctt gtggttccac gactgtgatc
     3961 tgatccacac ggtaagcaag ctttttatcc aatacatgta taaaaaagaa atctcccttt
     4021 ttcatctctg tcagatctgt aaagattttt gcagaactaa gcccagtatg tccagtcaac
     4081 accgcatggg tatcttttcc tccaatcgga aaggaagatg tagccagatg tccgatccca
     4141 tgttccaaaa ctgtcccact tgtcccatga tagatcggca gattaattga aatgcaagga
     4201 atctttaaat atcccatgat cgatgatcca tcaatattta atattctgtt ataatgaacc
     4261 gtctctccat ctaagcgttt ttccttaaac ggatcagtca actgtactga agatcgcatc
     4321 aattccccat tatactgttt tgcttcatcc attacttttt ttatgatcgc ctgatccatt
     4381 ccggctgcct tttcctgata actttccacc gtcgaacctg cactcttttc aaacagatag
     4441 ttgcttgtaa atggataaaa caacagcaaa aaacctgtca gaacgatcag aatcggtatg
     4501 atcttttgtt tcattttatc tttgtgatcg tcttctgttt ttgttcaaga tcaagatccc
     4561 acatccagca acaacaaccc ctgcaatgat cagaagatag tttcctgccc cacctgtcat
     4621 tggcagcatg aactgcttct gatttgtgat ctgcatattg atatatgcat ttccagaatc
     4681 atcgtgtttt gtcatcgttg cctttatctc atccacagtc gcactcgctg ctgtagtctg
     4741 aaccgtcaca tcattcttta ataccgcacc attaggaact ccatcatttg ctgtggaatc
     4801 tgcatcagac ttactctgga tccctgtgat gtttgcttcg gttggtgtga tatctgcttt
     4861 tgtctctttg accttaacga tcacttcttt ctttaacagg gagtatcctg catcggagtg
     4921 tgtttctgta aatccatatt catctccttc gatcccattg atcacaagtt ttccatcaga
     4981 agacggacta aactgagtcg catcagcttc ttttgcagac tttcctgtta cctgataaac
     5041 acctgctcta tctgctcttg ctaccagata gtaattatct gtcttatttt ttacaacgaa
     5101 tttaaccttt gttgcatctc ccttgctgtc agaaaatttc tttgtcagat catatccgta
     5161 agagtatacg atacatttgt cttttaaaat atcataataa ttcgtatttg ttctcttcca
     5221 cgtaagggat acatcgtttg gatttccttt atcaccaaga ataacgctgt catctgtgtt
     5281 caccttagct gtatagtata caacaatata tttatcagaa tatttcttat tcaactcatt
     5341 aagtcctgct tttgtcatct cgatcttcat tgcaggatcg tctccagatt ttccgtatgt
     5401 tgctgcaaac tttggatccg tgtcggaact cttccataca gccaatgctc cactcttttc
     5461 aacattattc acatttgttc tgtccgctgc atccttagtt tcatagattg cgatcacggc
     5521 atctttccca tatgtcattc cttttgccat cgtatcgtta aatgtataag ttgacaggta
     5581 ggttgttgat gatgtgatat gtggcaattt ggaaatgaac tgataatcca gtctttctgc
     5641 tttagatgct gtcacggtac tctgatattt gtattcattt cttgcagaag taaaatctgc
     5701 cagtctgtct gtattggcag ttgttacatt gtcctgatct ggattattgc gtactctttt
     5761 gtcaagtgtt ggatttccag tttcattctt tggataacag atcacatcat agaaccagtc
     5821 atctccctta gaatccgtgg atggcaactg tacaaaccat ggatttgttg tgtaagttac
     5881 attttctggt gcctttgtct caacgatcag gtaaagaccc agtggcaatt tgtctttact
     5941 tgtcacacca ttagcattag tctcatccat ggctgtccca ttctttccca tgtaatcttc
     6001 taatttgtct ttcgttaccg tattatcttc taatgccttg gcaagtttct cattgatctg
     6061 ctggcttgta aaatatgtct tacttccttc tgttttagct gcatcagaag atgtcaaacc
     6121 caaaatctgc tgtattgtgg ttggaagttc atagatcatc tggatctttc cattctcact
     6181 ctgctgttca acatctccaa ctcgcaggta actaaattcc acacctttga tcgcatattt
     6241 ttccaaggct gcttccgcag cagcgtcctg ttttcctgtt ggtgtaaact gactggtatt
     6301 aacaccggcc tgttttgctg ctgtcatgtc atatttgtgg atcgttatag atccggtttt
     6361 gcttgtatcg accacatctg ttgatgccgc tttgatatta acgataggtg caagcatcat
     6421 tgctgcagat aatacagcaa tcattatttt tttgatcata ttttgaatct tcatttttct
     6481 ttcctcctat tttttagaat agaaaaagca cagaaatcat tgatcatact caatgattct
     6541 gtgcttttct ttatgtaata tatactattt tatttatctg ttaaattagt tttgtcaggt
     6601 gcaatcatga tcgtattcca aaaagaatct ttgactgcct ctgcctgaat ctcatctact
     6661 actgtgtcaa gatcgacatc acttgttaac ttcttgctcg catccattac ggaaacttta
     6721 tcgttgattc ctaaggatgt gatcgtaaca aactgttctt cactaactag tgttcctttc
     6781 tttagtggaa ccgctaactg ataattcttc tgcaatactg acttgatctc attatacatt
     6841 gctttaaatt ctttgcttct gtatttcttc tcagccttca gctgctttgc cttcttatca
     6901 gccttcgcag atatcttttg atcagaataa atctcccagc atggacctcc tgctgatgct
     6961 gacttcgtct gtgcgatcac ttcttttagt ggatatttgt actgtgtacg ctgtctgctt
     7021 ccaacatctg caaccgttac ctgatcatta tcatcgatcg caaccaatac gataaaatga
     7081 cctttcttcg taaaatatcc tggccccatc attgctacta ctggatgctt cttcttcaat
     7141 gcatctctaa ccttgctgac atcacttcca agtccatgac attccagttt atactgttgt
     7201 gccagtgctg ggaccatctc atgacttgct ccagcactac tgtaatagcc attcttatat
     7261 gcccatactg ttgtatcaac tggagttacc catttattcg tcagcgaact gatacaaact
     7321 gccatagcgc ttggtccgca tccactgctt ttgatctgat atccattgtc attccatgct
     7381 gcatctgcct gattaaagta aataaatccc tgtatcgtct ctttcttatc tttcatctca
     7441 agactatgat cattatatac ttttggaatg atagattttt tatctttctt gtcttttttc
     7501 ttatcttttt tatctttctt cttatcttga tcagactttg tcttttgttt ctttgtatgc
     7561 gttaccgctg tatgtttctt tgtatgtgtt gttgctgtat gtttctttgt atgtgttgct
     7621 gcagccgttg tatgcgtatg cgttgttgct tccgttgtcg gctttgcctg agtcgtctgt
     7681 gttgttgtct ctgtcgttgc ttcactttct gtcgtctggg cagaagaatc tgtcttggtc
     7741 tcagtctgtt cactgcttat ttgctctctt gtgactggct gatctgctct gaccacagac
     7801 actgatccaa gcatcaaaaa cgtatacaga gctactacag ctgctgtttt acgtgttttt
     7861 ctcatctttc attgttcccc ctaactattt aaacatactt attataccac tttggatacc
     7921 ctcgtcaata aaaatgaatg gtcatgatcc atgttcacat ttttttcaca tcttatgctt
     7981 caaagactaa tttttcaatt gcatatgcaa caccatcttc attatttgtc tttgtcacaa
     8041 agtctgcgat ctctttcaga tcaggttgtc cattttccat aacaacaccg gttcctacag
     8101 ctttgatcat catcgtatca tttcctgcat ctccacatgc catcacttca tccatagata
     8161 gtccaatttg ctctgccaga tgtaaaagtc catctccttt atcacatcca atctgattta
     8221 tttcaatgtt attccccaga gaacttgata cactcgcaat atttttctcg attaattctt
     8281 gtcttgcagt ttcccgctct tccatatcat caaaaaatac attgatcttc tcaattccca
     8341 gcggatgctc ttcaatgtac tttcgcagat catccagcgg tagtcttgta tctctcacaa
     8401 gttttaatac atgatctgat acaccatact ctgcaagatg gtcaaaatag catcgttcca
     8461 tatacccttg cccattctga taaatatcac gtgtacaatg atatttttcc aaaatatcaa
     8521 acaacgaaaa taccgtttct tttggtagat gatttgtata tatgatctta tcttctttct
     8581 gatcataaac agttgccccg ttacagcaaa cagcatatcg aacgcctgga atatctctta
     8641 aaaattctgg aattgcttta aaaatacgtc ctgttgctgg aactatataa acaccccttc
     8701 tgatcgcttc ttttagaact tcttctgtgt gttttgaaat ctgtttctcg ctgtctaaga
     8761 gtgtattatc tagatccatc gctactaatt ttatcttact catgctattc ct
//
LOCUS       DAOMUE010000048         8770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2045_length_8770_cov_3.139300, whole genome shotgun sequence.
ACCESSION   DAOMUE010000048 DAOMUE010000000
VERSION     DAOMUE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2045_length_8770_cov_3.139300"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..851
                     /locus_tag="PLC71_03670"
     CDS             144..851
                     /locus_tag="PLC71_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01776.1"
                     /translation="MIKNIMGAAIAVALAVGIGTTGVISNTTYKKPTQIIAAAKTTTL
                     TDYHSNTEFTKSSDVKKIISTYGLKDSDKTSKIVYIPINNYSVNDADGQNNEVPVDTR
                     LANYSVKKKGYKDQVSYVRSITANTSLAQSIKESVTTTYDFSNIVSVSGKNAALESCL
                     TSAYGFSVSNRSNMNETFKLSSENGADLNIYVLDRTYDYQLWETDLSHDISSDSYLGN
                     GTIKRPVGLIITVSKNS"
     gene            1006..2589
                     /locus_tag="PLC71_03675"
     CDS             1006..2589
                     /locus_tag="PLC71_03675"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRM01777.1"
                     /translation="MVTWNNLDTLTSYAKLKDLKNHVDIKEAMAGENGAKRVAEYSAP
                     MASGLSFNYAAKQVDDEVLDVLAELADEHQLVDKFEELYNGAIINTGEQRLVLHHLAR
                     RQLGKDVIVDGVNKRDFYVEQQKKAADFANKVHAGEITNEAGETFTTVVQIGIGGSDL
                     GPRALYIALENWAKTNGCAKMEAKFISNVDPDDAAAVLNSIDVSRSLFIVVSKSGTTL
                     ETLTNEAFVKDALVKAGLNPSKHMLTATSETSPLAKSDDYLAAFFMDDFIGGRYSSVS
                     SVGGVILSLAFGPDVFARILDGMAEEDKLATNKDIKANPDLLDALIGVYERNVQGYPE
                     TAVLPYSQALSRFPAHLQQCDMESNGKSVNRFGEPVDYVTGPIIFGEPGTNGQHSFYQ
                     LLHQGTDIVPLQFVGFKDSQLATDVVIEGSTSQKKLCANVAAQIVAFACGKDDENNNK
                     KFEGGRPSSIIIGDQLTPESLGSLLAHFENKIMFQGFIWNVNSFDQEGVQLGKVLAKR
                     VLAHETEGALKAYSDLFEI"
     gene            2752..4251
                     /locus_tag="PLC71_03680"
     CDS             2752..4251
                     /locus_tag="PLC71_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRM01778.1"
                     /translation="MKKQIFRNLCRFLVFILMFSCFFVFGKKVSANENQNAAPIITYY
                     GLNGNDITIQWKAPDGVSYSKVDIYSATSPNGSYRYENTVSGNSYTNTYVAKGVPYYY
                     KLEASYEDNEGYKTATTYAGKCIINPLPAPSSLEANTGNSHSITVKWKASDDCDGYQI
                     YRSVSPDGNYELVQTVSNESRSSWWYDDDDESFQTYTQKNLELGKIYYYKIRPYTTYI
                     DETFYGDFSNYISCQVTINGTKVKSASSKKKKINTITWAKNDEADGYIVYYSKKEDGN
                     YTKLKTFTSRNNLSYTHTKLTNGTAYYYKIQAYKNFNGGKLYGPMTPYLKYCDYYSYA
                     DESYESRCRRAFGKSYYADYKSAKQAKKHMKTITVKVWDKKGKKKYTRKFRITVNKGL
                     APSIKEMFKEIYKSKERFPIHEIGCYSWRGKNSSSEHCEGLAFDINSNENYMIQGKKV
                     LAGSFWKPKKNRYSIPLNCKLVKILEKYGFHRGLWGSRRDYMHFSYFGG"
     gene            complement(4300..4372)
                     /locus_tag="PLC71_03685"
     tRNA            complement(4300..4372)
                     /locus_tag="PLC71_03685"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4337..4339),aa:Thr,seq:cgt)
     gene            complement(4399..4662)
                     /locus_tag="PLC71_03690"
     CDS             complement(4399..4662)
                     /locus_tag="PLC71_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRM01779.1"
                     /translation="MISKNVKVEVALREEDRPIAFLVQMASQFESKILFETGNKQVNV
                     KSMMGMMSLGLDLDRLEDLTVVADGVDEEEAISKIEYYLTKGA"
     gene            complement(4659..5585)
                     /gene="whiA"
                     /locus_tag="PLC71_03695"
     CDS             complement(4659..5585)
                     /gene="whiA"
                     /locus_tag="PLC71_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HRM01780.1"
                     /translation="MSFSSNIKEELSRNETGARHCKIAEIAGFLRFGGNISLEPEHYE
                     IQIQTETVSAAKRFYTLIKDVFDIHAKIDVGRNEFLKRSRNYTISVDKHKDCVLILKA
                     VKLLDSKEFGLVLQNTCCKRAYIRGAFQAAGSMSDPEKNYHFEVVSTNVQTAEQLKEV
                     LNFFDLDAKIVLRKKYYVVYMKEGSKIVDVLNIMEAHVALMELENVRILKDMRNKVNR
                     RVNCETANINKTVSAAVKQVEDIEYIDKHKGLRFLDQGLQDIARLRLEHPEATLKELG
                     DMLEPPVGKSGVNHRLKKIGRIAEEIRLGGQE"
     gene            complement(5589..6443)
                     /gene="rapZ"
                     /locus_tag="PLC71_03700"
     CDS             complement(5589..6443)
                     /gene="rapZ"
                     /locus_tag="PLC71_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRM01781.1"
                     /translation="MRFVIVTGMSGAGKSSALKTLEDAGYFCVDNLPVALLDKFADML
                     LDETAKIENVALGIDIRNGRGIGELAHKLKEIEAAGIKYEILYLNASNRILLKRYKET
                     RRNHPLSRDGRVEDGIAKEREIMKFLQDQATYVIDTSQLLTRELKEEIDRIFVDGEEG
                     ERFQIAVVSFGFKHGIPADVDLVFDVRFLPNPYYDLNLRPLTGNDKPIQDFVMKHEES
                     VEFLDKLDDMMRFLIPNYIKEGKYNLVIGIGCTGGKHRSVTITNKLAERLKQLPYSVK
                     VEHRDIMR"
     gene            complement(6459..7367)
                     /gene="murB"
                     /locus_tag="PLC71_03705"
     CDS             complement(6459..7367)
                     /gene="murB"
                     /locus_tag="PLC71_03705"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRM01782.1"
                     /translation="MDTKIDQLKQIIPEKYILRQEPMKNHTTFRIGGPADIFVAPENM
                     EEIKAVSRFAKEEGIPLFVLGNGSNLLVADDGMDGIVLQIYKNYSGIEVRGNELIVKA
                     GTLLSSTSRAALNEELTGFEFAGGIPGTFGGAVVMNAGAYGGEMVQVLKEVTVLTKEG
                     EIKTLKAEELELGYRTSNVLKNEYVVLEGVIALKKGNKEEIKAKMDEYALARKTKQPL
                     EYPSAGSTFKRPEGYFAGKLIQDAGLKGYQVGDAQVSEKHSGFVINRGNATASDVMQL
                     ISDVKDKVKEQFGVTMEPEVKRVGRF"
     gene            complement(7437..8372)
                     /locus_tag="PLC71_03710"
     CDS             complement(7437..8372)
                     /locus_tag="PLC71_03710"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HRM01783.1"
                     /translation="MKYLYGIDIGGTTVKMGLFGEDGTLKEKWEIKTRTEENGKNILP
                     DIAQAVNDHSKANGFDKEDVIGLGVGVPGAVLEFSKVNECVNLGWGSVDVAGELSKLT
                     GCKVKATNDANAAALGEIWMGAAADYNSAVMITLGTGVGGGIIVDGKIIDGSRGYGGE
                     IGHMTVDPFDDRVCNCGKTGCLELYASATGIVYETKKALKDYKEATTLRDLDEVTAKD
                     IFDAAKEGDTFAKERVDDLGQKLALAAGNIALMVDPEVFVIGGGVSRAGQILLDAVNA
                     HFKKYTFGKAQETGFVLATLGNDAGIYGAASLMLS"
     gene            complement(8378..>8770)
                     /locus_tag="PLC71_03715"
     CDS             complement(8378..>8770)
                     /locus_tag="PLC71_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRM01784.1"
                     /translation="DDVVEIKRVSKKSLIGTSPEVTRFFIELRGIGIIDVKNLYGVES
                     VKMEQEINLVIQLEDWNKDADYDRLGLEEKYVEYLGNKVAAHTLPIRPGRNLAIIVEA
                     AAINHRQKKMGYNAAEELYKRVTGQMED"
BASE COUNT         2599 a         1866 c         1430 g         2875 t
ORIGIN      
        1 cctattaaat ttggttttat tacacaaaac gctgaatctt cgaatttttc ttgacttgga
       61 ttgtatattg tatttataat gtacccaatt taagaaaatg aagattaata cttacaatta
      121 cacgaaagga accaagaata tatatgataa aaaatatcat gggtgctgcc atcgctgttg
      181 ctctcgcagt tggtatcggt acaaccggag taatatcaaa cacaacttat aaaaagccaa
      241 cacagatcat agcagctgcc aaaacaacaa ctttaactga ttatcattcc aatacagaat
      301 tcacaaaatc ctctgatgtg aagaagatca tttccactta tggtttaaaa gattctgata
      361 agacaagcaa gatcgtatac attccaatca ataattattc tgtgaacgat gcagatggtc
      421 agaataatga agttcccgtt gatacaagac ttgcgaacta ttctgtaaag aaaaaaggat
      481 acaaagatca ggtttcttat gtaagatcaa tcactgctaa tacttcttta gctcagtcga
      541 tcaaagaatc tgtaacaaca acttatgatt tctcaaatat cgtatctgta tctggaaaaa
      601 atgcggcatt agaatcatgt ttaacatctg catatggttt ttctgtctca aacagatcaa
      661 atatgaatga aacttttaaa ctttcctcag aaaacggtgc tgatttaaat atctatgttt
      721 tagatcgtac ttatgactat cagctttggg aaactgatct gtctcatgac atttcttctg
      781 acagctatct tggtaatgga accataaaac gtccagttgg tctcatcatt actgttagca
      841 aaaattcata aaatgagtta atacttagtc ccatttcaga ttgcatttac agcgtttttt
      901 ttgtataatt attctatgat attagtgtgt gcagataccc atttgtatct gcatttcctt
      961 atatctttca ttttatattt atatggactt aaggaggatt tatttatggt aacatggaac
     1021 aatcttgata ctttaacatc ttatgctaaa ctcaaagatc ttaaaaatca tgtagacatc
     1081 aaagaagcta tggcaggtga aaacggtgcg aaacgtgtcg ctgaatattc tgcacctatg
     1141 gcttctggtt taagctttaa ctacgctgca aaacaggtag atgatgaagt tcttgatgta
     1201 ttagctgaat tagctgatga acatcagtta gttgacaaat tcgaagaatt atacaacgga
     1261 gcgatcatca acacaggaga acagagactt gttcttcatc atttagcccg cagacagctt
     1321 ggaaaagacg ttatcgttga tggtgtcaac aaacgtgatt tctacgttga acagcagaaa
     1381 aaagcagctg actttgcaaa caaagtacat gctggtgaga tcacaaatga agctggtgaa
     1441 acattcacta cagttgttca gatcggtatc ggtggtagtg atctaggacc tcgtgcttta
     1501 tatatcgctc ttgaaaactg ggctaagaca aacggatgcg ctaaaatgga agcaaaattc
     1561 atcagtaacg tagaccctga tgatgcagct gctgttttaa attctatcga tgtttcccgt
     1621 tctttattta tcgttgtatc taaatctgga acaacattag agacattaac aaatgaagct
     1681 ttcgttaaag atgctttagt aaaagctgga ttaaatcctt ctaaacatat gttaactgca
     1741 acaagtgaga cttctccttt agctaagagt gatgattatc ttgctgcatt cttcatggat
     1801 gactttatcg gtggacgtta ttcttctgta tcttctgtag gtggagtgat cctttcactt
     1861 gcatttggac cagacgtctt tgcaagaatc cttgatggta tggctgaaga agacaagctt
     1921 gctactaaca aagacatcaa agcaaaccct gacttattag atgccctgat cggtgtttat
     1981 gaaagaaatg tacagggata tcctgaaaca gctgttttac catattctca ggcattaagc
     2041 cgtttcccag cacatttaca gcagtgtgac atggaatcta atggtaaatc tgtaaaccgt
     2101 tttggtgaac ctgtagatta tgtaacagga cctatcatct ttggagaacc tggtacaaat
     2161 ggacagcatt ctttctatca gttattacat cagggaactg acatcgttcc tttacagttc
     2221 gttggattca aagacagcca gcttgctaca gatgttgtga tcgaaggaag cacaagccag
     2281 aagaagttat gtgctaacgt tgcagctcag atcgttgctt tcgcatgtgg taaagatgat
     2341 gaaaacaaca acaagaaatt cgaaggtgga cgtccatcaa gtatcatcat cggagatcag
     2401 ttaacacctg aatcacttgg atctctgtta gctcatttcg agaacaagat catgttccag
     2461 ggattcatct ggaatgtaaa cagctttgac caggaaggtg ttcagcttgg taaagtactt
     2521 gcaaaacgtg tacttgcaca tgaaactgaa ggagcactga aagcttatag tgatttattt
     2581 gagatttagt ttcctggatc agtagatact atttactgta tcgacacttg taagctaaaa
     2641 tagaatatta aaaaccacag tatgcatttt tcattgattt ttgcattact gtggtttttt
     2701 tatgttataa tgagaccgta tagaatatat atgaagaaag gggaatataa attgaaaaaa
     2761 caaattttta ggaatctatg cagatttttg gtatttattt taatgtttag ctgttttttt
     2821 gtctttggta agaaggtttc tgccaatgaa aatcaaaatg ctgcaccaat tattacttat
     2881 tatggattaa atggtaatga tataaccata caatggaagg cacctgatgg agtttcttat
     2941 tctaaagtag atatttattc agcgacttcc ccaaacggtt cttatcgcta tgaaaatacc
     3001 gtatctggca attcctatac aaacacctat gttgcaaaag gtgttcctta ttattataaa
     3061 ttagaagctt cttatgaaga taatgaggga tataaaactg caaccactta tgcaggcaaa
     3121 tgtattatta atcctttacc agctccttct tctttggaag ccaacactgg aaattcacat
     3181 agtattactg ttaaatggaa agcatccgat gattgtgatg gttatcaaat ttatcgaagt
     3241 gtttctcctg atggaaatta tgagttagtc caaactgtat ctaatgaaag tagaagttct
     3301 tggtggtacg atgatgatga cgaatctttc caaacatata cgcaaaaaaa tcttgaacta
     3361 gggaaaatat attattacaa gatccgtcca tacactacat atattgatga aacattttat
     3421 ggggattttt ctaattatat ttcttgtcag gttacgatca atggtacgaa agtaaaaagt
     3481 gcttcttcca agaaaaaaaa gatcaatacg atcacatggg caaaaaatga tgaggctgat
     3541 gggtatatcg tatattattc caaaaaggaa gatggaaatt acacaaaatt aaagactttc
     3601 acaagtcgaa acaacttatc ttatactcat acgaaattaa caaacggtac tgcttattac
     3661 tacaaaattc aggcatataa gaactttaat ggcggtaaac tgtatggtcc aatgacacct
     3721 tatctaaaat actgtgatta ctattcttat gcagacgaat cttatgaaag tcgctgccgc
     3781 cgtgcttttg ggaagtctta ttatgctgac tataagagtg ctaagcaggc aaagaaacat
     3841 atgaaaacga tcacagtaaa agtatgggat aagaaaggta agaaaaaata tacaagaaaa
     3901 ttccgtatca ctgtaaacaa agggcttgca ccttctatca aagaaatgtt taaggaaatc
     3961 tacaagagca aagaacgttt ccctatccat gagatcgggt gctacagctg gcgtggtaag
     4021 aattccagtt ctgaacattg tgagggatta gcttttgata ttaactccaa tgaaaactat
     4081 atgattcagg gtaaaaaggt attggctggt tctttctgga aacctaagaa gaatcgttat
     4141 tcaattcctt tgaactgtaa attggttaaa atcctcgaga aatatggttt tcatcgtgga
     4201 ttgtggggat cccgcagaga ttatatgcat ttttcttatt ttggtggtta atacaaatca
     4261 aacaaaggat caggcatttg cctgatcctt tgttatgtaa gccgaaaacc ggacttgaac
     4321 cggcgacccc ttcattacga gtgaagtgct ctaccaactg agctatttcg gcatatatga
     4381 aagcacagat atgtgtatct atgctccttt tgtaagataa tattcaatct tgctgattgc
     4441 ttcttcttca tcgactccgt cagctacgac tgttaaatcc tctaaacgat ctaagtctaa
     4501 tccaagactc atcattccca tcatactctt tacattgact tgcttattcc ctgtctcgaa
     4561 taaaatcttg ctctcaaact ggctcgccat ctgtacaaga aaagctattg gtctgtcttc
     4621 ttctcttaag gctacttcca ctttgacgtt ctttgaaatc attcctgtcc tcccaatctt
     4681 atctcttccg caattcggcc gatcttcttt aaccggtggt tcacaccaga cttgcctact
     4741 ggcggttcta gcatatctcc aagttctttc agtgtagctt ccgggtgttc cagacgtaat
     4801 cttgcaatat cctgcaaccc ttgatctaaa aaacggaggc ctttgtgctt atcaatatat
     4861 tcaatgtctt ccacctgctt gactgcggct gaaaccgtct tattaatatt cgctgtctcg
     4921 caattgacac gacggtttac tttattacgc atatctttca ggattcttac attctccagc
     4981 tccatcaatg cgacgtgtgc ttccataata ttcaacacat ctacaatctt tgatccttct
     5041 ttcatgtata ccacgtagta ctttttacgc aagacaattt tagcatctaa atcaaaaaaa
     5101 ttcaagacct cttttaattg ttctgctgtc tgtacattag tagatacaac ttcaaaatga
     5161 taattcttct ctggatcact catggaacca gctgcctgaa aagcccctcg aatatatgcc
     5221 cgcttacaac atgtgttttg taaaacaagt ccaaattctt tggaatctaa aagtttcact
     5281 gctttcaata ttaaaacaca atccttatgc ttgtcaacac ttatcgtata atttcgactt
     5341 ctttttaaaa attcgtttcg tccaacatca atcttcgcat gaatatcaaa cacatctttg
     5401 ataagtgtat aaaaacgttt cgcggcagat accgtctccg tctggatctg aatctcataa
     5461 tgttctggct ctagactgat attccctcca aaacgtaaga atcctgcgat ctccgcaatc
     5521 ttacagtgtc tggcaccggt ttcatttctt gataattcct ctttaatatt acttgaaaat
     5581 gacattgcct acctcataat atctctatgc tctactttga cagaatatgg cagttgtttt
     5641 aatctctctg ccaatttatt ggtgatcgta acagatcgat gctttcctcc agtacatccg
     5701 attccgatca caagattata cttgccttct ttaatataat ttgggatcaa gaatctcatc
     5761 atatcatcta acttatcaag aaattccaca gattcttcat gcttcatcac aaaatcctga
     5821 attggtttgt catttccagt taatggtcgc agattcagat catagtatgg atttggaaga
     5881 aatctgacat caaataccag atctacatcc gcaggaatcc catgtttaaa tccaaaagaa
     5941 acaactgcaa tctggaatcg ttccccttct tctccgtcta caaaaatacg atcgatctct
     6001 tcttttaact cccttgtcag aagctggcta gtatcgatca catacgttgc ctgatcttgc
     6061 agaaatttca tgatctcccg ctcttttgca attccatcct caactcttcc atctcttgac
     6121 agaggatgat ttcttcttgt ctctttatag cgttttaata aaatacgatt acttgcattc
     6181 agatagagaa tctcatactt gatccccgct gcctcgattt cttttaattt gtgtgctaac
     6241 tcgccaattc ctcttccatt acggatatca atcccaagag caacattttc aatcttggca
     6301 gtttcatcta acaacatgtc tgcaaattta tcaagtaaag ccactggaag attatcaaca
     6361 caaaaatatc cagcatcttc taatgtcttt aatgcactgc tttttcctgc tcctgacatt
     6421 cctgttacaa ttacaaatct catctgtact gctccttttt agaaccttcc aacacgtttg
     6481 acttctggct ccatcgtaac tccaaactgt tcttttactt tatctttcac gtcactgatc
     6541 aactgcataa catcggatgc tgtagcattt ccacggttga tcacaaaacc actgtgtttt
     6601 tcagatacct gtgcatctcc cacctgatat ccctttaatc ctgcatcctg aatcaatttt
     6661 ccggcaaaat atccttctgg acgtttaaat gtacttccgg cacttggata ttctaatggc
     6721 tgttttgttt ttctcgccaa tgcgtactca tccattttgg ctttgatttc ttctttattt
     6781 cctttcttaa gtgcgatcac accttctaag acaacgtatt cattctttaa aacgttactt
     6841 gtacggtaac caagctctaa ttcttctgct ttcagagttt tgatctctcc ttcttttgta
     6901 agaactgtca cttcttttag tacctgcacc atctcaccac cgtatgcacc agcattcata
     6961 acgacagctc cgccaaatgt tccaggaatt ccacctgcaa actcaaatcc agttaattct
     7021 tcattgagtg ctgctctgga tgtagaactt aataaagttc ctgctttgac gataagttca
     7081 ttaccacgga cttcgattcc actgtagttt ttgtaaatct gaagaacaat tccgtccatt
     7141 ccatcatctg cgaccaaaag attactccca tttcctaaaa caaataatgg aattccctct
     7201 tcttttgcaa aacgactgac cgctttgatc tcttccatat tctctggtgc cacaaaaata
     7261 tcagcgggtc ccccaatacg gaatgttgta tgatttttca tcggttcctg tcttaaaata
     7321 tatttttctg gtatgatctg ttttaactga tcaatctttg tatccataat ttctccattc
     7381 ttaatattaa tgcaagatgg aatcctgtat atcacaggat tccaaatctc ttcatcttat
     7441 gaaagcatca gactagctgc accataaatt ccagcatcat ttccaagagt cgcaagtaca
     7501 aatcctgtct cctgtgcttt tccaaatgta tacttcttaa aatgtgcatt gaccgcatct
     7561 aaaaggatct gtccagctct tgatacgcct ccaccgataa caaacacttc tgggtcgacc
     7621 atcaatgcga tattccctgc tgcaagtgct aatttctgtc caagatcatc tacccgttct
     7681 ttggcaaatg tatctccttc tttagcagca tcaaaaatgt cttttgcagt tacttcatca
     7741 aggtctctta gtgtagtcgc ttctttataa tcctttaatg ctttctttgt ctcatataca
     7801 attcccgttg cagatgcata aagttccaga catcctgtct ttccacaatt acatacacga
     7861 tcatcaaaag gatccacagt catatgaccg atctctccac catatccacg gcttccatcg
     7921 atgatctttc catcaacaat gattcctcct ccaactcctg ttccaagagt gatcatcacc
     7981 gcgctgttat aatctgctgc tgctcccatc cagatctctc ctaaggctgc tgcattggca
     8041 tcattcgttg cctttacctt acatcctgta agctttgaca attctcctgc cacatccacg
     8101 cttccccatc caaggttcac acattcatta acttttgaaa actcaagaac agctcctgga
     8161 actccaactc cgagtccgat cacatcttcc ttatcaaaac catttgcttt gctgtgatca
     8221 ttcactgcct gtgcaatatc tggcaggatg ttctttccat tttcctcagt tcttgtcttg
     8281 atctcccatt tctcttttaa tgttccatct tctccaaaca gacccatttt tactgttgtt
     8341 ccaccaatat caattccata taaatatttc atatcattta atcctccatc tgacctgtaa
     8401 cacgtttata aagttcttcg gctgcattat atcccatctt cttctgtctg tgattgatcg
     8461 ctgctgcttc tacgatgatc gccaagttac gccctggacg aattggcagt gtatgtgctg
     8521 caactttatt gccaagatat tctacgtatt tctcttctaa tcctaagcgg tcataatccg
     8581 catctttgtt ccagtcttcc aactgaatga ccagattgat ctcctgctcc atcttaacgc
     8641 tttcaacacc atacagattc ttaacgtcaa taataccgat tcctcgcagt tcgataaaga
     8701 accttgtaac ttccggagat gttccgatca atgacttctt gctgaccctc ttgatctcta
     8761 caacgtcatc
//
LOCUS       DAOMUE010000049         8756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2050_length_8756_cov_2.478566, whole genome shotgun sequence.
ACCESSION   DAOMUE010000049 DAOMUE010000000
VERSION     DAOMUE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8756
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2050_length_8756_cov_2.478566"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="PLC71_03720"
     CDS             <1..1207
                     /locus_tag="PLC71_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009264403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HRM01785.1"
                     /translation="GKVHNTVASLTGAACLVLTHILSIEDCIDAVDVETICILVGMML
                     LVAVIKNSGIFEYIAIKAAKIAKGRPWPIMVIFILITAVCSGMLDNVTTVLLVGPMTL
                     AITNILKVDPVPYIITQIMASNIGGTATLIGDPPNIMIGSAAKLSFVDFILNTGVATV
                     FVIIVGLICMYFIYGRKLFVTDESIAKVMQLDENKAIKDRKLMHESVIVIILVALCFI
                     FHDQLGVQSCTVAIAAACIMLLIGGQEPEEIIADVEWPTILFFIGLFIVVGGMKKVGV
                     ITMLANGLISITHGNMVVTMMVILWVSAIVSSFLDNIPFVATLIPMILTMQSEGMDVA
                     PIWWALSLGACLGGNGTLIGASANVVLSGISKNNGHPITFGQYFKIGFPMMILSIIVC
                     SVFLLIRFA"
     gene            1252..1722
                     /locus_tag="PLC71_03725"
     CDS             1252..1722
                     /locus_tag="PLC71_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01786.1"
                     /translation="MNQNTVTGDYININLRQLGATSIKEFNSIMHIAEFDLGDGLIVS
                     YVFNITRHNKYFLQRMQPYAMVHGKFASDQEIIDFITADLKRFENAKNSHNFNTFLLI
                     SHLGHHITENIEQLFLNYNVDADKLESIEDSLEESLVEIQSLFEKSDKLDLPED"
     gene            complement(1850..2077)
                     /locus_tag="PLC71_03730"
     CDS             complement(1850..2077)
                     /locus_tag="PLC71_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRM01787.1"
                     /translation="MKHLLEVIAKALVDQPDKVTVTEKETADAVVLELHVAEGDMGKV
                     IGKQGRIAKAIRTVVKASVDKGDKKIVVDIQ"
     gene            complement(2103..2348)
                     /gene="rpsP"
                     /locus_tag="PLC71_03735"
     CDS             complement(2103..2348)
                     /gene="rpsP"
                     /locus_tag="PLC71_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRM01788.1"
                     /translation="MAVKMRLKRMGQKKAPFYRVVVADARSPRDGRFIEEIGTYDPNQ
                     DPSVIKFDEEAAKKWLATGAQPTDTVAKLLKAAGIEK"
     assembly_gap    2523..2532
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2533..3723)
                     /gene="ffh"
                     /locus_tag="PLC71_03740"
     CDS             complement(<2533..3723)
                     /gene="ffh"
                     /locus_tag="PLC71_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRM01789.1"
                     /translation="MAFESLSEKLQGVFKNLRSKGRLTEDDVKAAMREVKRALLEADV
                     NFKVVKTFIKSVQERAVGQDVLNGLNPGQMVIKIVKEEMEALMGSTMTEIQLRPGNEI
                     TIILMAGLQGAGKTTTCAKLAGQYKKKGKRPLLVACDVYRPAAIKQLQVNGEKVGVPV
                     FTMGDKQNPVDIAKASVEHAQKNGNNIVILDTAGRLHIDENMMTELVEIKENVDVFQS
                     ILVVDAMTGQDAVNVAKEFNEKVGVDGIIITKMDGDTRGGAALSIRSVTGKPILYVGM
                     GEKLEDLQQFYPDRMTSRILGMGDVLSLIEKAEQTINEEDALRMTEKMKKAEFDFNDF
                     LDQMDQMKKMGGLSNLLSMIPGVGNKMQGLDIDDGMMDKTAAIIYSMTKEERANPKII
                     NASRK"
     gene            complement(3742..4095)
                     /locus_tag="PLC71_03745"
     CDS             complement(3742..4095)
                     /locus_tag="PLC71_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRM01790.1"
                     /translation="MERIVEQNLLYDFYGELLTAHQKEIYGEHILNDMTATEIAAEFG
                     ITRQAVHDMIRRCDKILADYEARLHLVAKFLTAKEKIKEIHTLSRRLLKEDKEQMEED
                     IRQIESVCNQVLEDL"
     gene            4269..5866
                     /locus_tag="PLC71_03750"
                     /pseudo
     CDS             4269..5866
                     /locus_tag="PLC71_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392934.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(5929..7143)
                     /locus_tag="PLC71_03755"
     CDS             complement(5929..7143)
                     /locus_tag="PLC71_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM01791.1"
                     /translation="MSNINISYKELKQLEKESYQLLDMRDESNTSYGMIPGAVVAAGE
                     DELGTQAKEYIDQGKKVILYCTKGIFSKEAAEKLAEEGINVLSLEGGYTGWLLSLMKE
                     EQENDQKTEQNNKEAEGKGKKKELTRTQEIEKSIRKKFHKQIFSKFVKAIKTYDLVQE
                     NDKIAICISGGKDSMLMAKLFQELKRHNKFHFDLVFLVMDPGYNEMNRQIIENNAKLL
                     DIPITVFESDIFDAVYEIEKSPCYLCARMRRGYLYSKAKELGCNKIALGHHYDDVIET
                     ILMGMLYGAQVQTMMPKLHSTNFEGMELIRPMYLIREDDIKHWRDYNGLHFIQCACKF
                     TDTCTTCNPDGVTKSKRMETKQLIAKMKEINPYVESNIFKSVENVNLKTIIAYKKDGV
                     KHSFLDTYDQEN"
     gene            complement(7245..8066)
                     /locus_tag="PLC71_03760"
     CDS             complement(7245..8066)
                     /locus_tag="PLC71_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01792.1"
                     /translation="MKKQIRNALLVVAALLLVVLFYFLPNIQAALVDKKTFGQSEDLS
                     LNPVEISVEATQTTLEKLKIINNHILSVAVRPSSRTVDGRDVEKTAKKQTNLLLKKMK
                     IAYRVEEDWKITYKKLYTYIKKKDDMIDDGSVSNISNVKNLLVWYITLSPESSEDEIV
                     NLIMDAYTDQILAIDVYSYDYTKDWKDIADHLEDIPAGFLSYLNLKQSEYNLKKRLMG
                     NVNVTEQYQKAKSAKGSYSQISGRIGIYAIQNKKQTYIPIEWSGYGFMINNVYNN"
     gene            complement(8050..>8756)
                     /locus_tag="PLC71_03765"
     CDS             complement(8050..>8756)
                     /locus_tag="PLC71_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM01793.1"
                     /translation="MKLDQLLKNQEEFMGSFAHEMKTPLTSIIGYADLMRMEALSKEE
                     QKEACGYIYSEGKRLQNLSLKLMKLLVLKNQQFQMKKQDLEPMIQEAVTSMQYRLKEQ
                     DILVNLNLKKAICKIEPDLLKSLILNLIDNALKSMNSGGILTVEDRAIQEGAKIYISD
                     TGCGMPKDEISKITEAFYRIDKSRSRKQGGVGLGLAICKEIVRIHQGEMRFISQQGKG
                     TTVIITIGGGAYEKTN"
BASE COUNT         2482 a         1836 c         1403 g         3025 t
ORIGIN      
        1 aggaaaggtc cacaacacag ttgcctcatt aaccggtgct gcctgtctgg tcctcaccca
       61 cattctttcg attgaagatt gtattgacgc agtcgatgtt gaaacaatct gtattctagt
      121 cggaatgatg cttttggttg ctgttatcaa gaattctggt attttcgaat acatagcgat
      181 caaagctgca aaaatcgcaa aaggtcgtcc atggccaatc atggtgatct ttattttgat
      241 caccgccgta tgttctggaa tgcttgataa cgttaccaca gtcttgctag taggaccaat
      301 gactctggca atcacaaata tcttgaaagt tgatcccgta ccatatatca tcacacagat
      361 catggcttcc aatatcggtg gtaccgctac actgatcggt gacccaccta acattatgat
      421 cggtagtgcg gccaaattaa gtttcgtaga ttttatttta aatacagggg tagccactgt
      481 atttgttatc attgttggtc tgatctgtat gtattttata tacggaagaa agttatttgt
      541 tacagacgaa tcaattgcaa aggttatgca gttagatgaa aacaaagcga tcaaggaccg
      601 aaaacttatg catgaaagtg ttatcgttat catcctagtc gcattatgct ttattttcca
      661 tgaccagtta ggcgtacagt cttgtactgt tgctattgct gcagcatgta tcatgctttt
      721 aatcggcggt caggaacctg aagaaatcat tgcagacgtt gaatggccta ccattctttt
      781 ctttattgga ttatttattg ttgtcggtgg gatgaaaaaa gtaggtgtca tcacaatgct
      841 tgcaaatgga ttgatttcta tcactcatgg taatatggtg gttacaatga tggtaatctt
      901 atgggtttcc gctattgtat cttctttcct cgacaacatc ccatttgttg caacattaat
      961 ccctatgatt cttacgatgc agtccgaggg aatggatgtc gctccaatct ggtgggcatt
     1021 gtcactcggt gcttgtttag gtggaaatgg aacactgatc ggtgcttctg caaacgttgt
     1081 tttgtctggt atcagtaaaa ataacggaca tccaattaca ttcggtcaat attttaagat
     1141 cggattccca atgatgatct tatcgatcat cgtatgttcc gtatttcttt tgattcgatt
     1201 tgcttaataa aaaattatgg attataatat ttaggagagg agcgtgatct tatgaatcaa
     1261 aataccgtaa ccggagatta tattaatatc aatctgcgtc agttaggcgc tacttctatc
     1321 aaggagttta acagcatcat gcacattgct gaattcgatc ttggtgatgg attaattgtt
     1381 tcttatgttt ttaatattac acgacataat aaatactttt tacaacgtat gcagccatat
     1441 gcaatggtcc atggtaaatt cgccagtgat caggaaatta tcgattttat tacagcagac
     1501 ttaaagcgtt ttgaaaatgc aaagaatagt cacaatttta atactttcct tttaatttcc
     1561 catcttggtc atcatattac agaaaatatc gaacagctgt ttttaaatta taacgttgat
     1621 gcagataaat tagagtccat tgaggattct ctggaagaat ctcttgttga aattcaatcc
     1681 ttatttgaga aatctgataa acttgatctg ccagaagatt aatatttata aaaattgccc
     1741 ttgaaatgaa atggtttcaa gggctttctt actttctcga atttatacat aacaaaaagc
     1801 ccacagtttt atacatcttc tgtacacttt cctgtgagct tttaatcttc tattgaatat
     1861 caacgacaat cttcttgtca cctttgtcaa ccgaagcctt gacaacagtt ctgatcgcct
     1921 ttgcaatcct tccctgtttt ccaattactt tccccatatc accttcggca acatgaagtt
     1981 ctaatacgac tgcatcggca gtttctttct ctgtaactgt cactttatct ggctgatcaa
     2041 cgagggcttt tgcaatcact tctaataaat gtttcatgca ctgcctcctg tcttaaatct
     2101 gactattttt cgattcctgc agcttttaat aatttagcta ctgtatctgt aggctgagca
     2161 cctgttgcta accatttctt agcagcttct tcgtcaaatt tgatcacgct aggatcctga
     2221 tttggatcat atgttccgat ttcttcaata aatctaccat ctctagggga tctagcatct
     2281 gctactacta ctctataaaa aggtgctttc ttctgtccca ttctttttaa tctcattttt
     2341 actgccattt tgtttttacc tccaaaaatc tttctttgta atttaaatta attattattt
     2401 tctataacta aggcctaaaa aggtaattta aatccacctc ttttaccacc tttgccacca
     2461 aacatacctg acatctgctt catcatctgt ttctgattct gaacctgttt gcataagcgg
     2521 ttnnnnnnnn nnctttcttg aagcattgat gatcttagga ttagcacgtt cttctttagt
     2581 catggaataa atgatcgccg ctgtcttatc catcatacca tcatcaatat caagtccctg
     2641 catcttgttt ccgacaccag ggatcataga taacagattg gataatccac ccatcttctt
     2701 catctgatcc atctgatcaa gaaaatcgtt aaaatcaaac tcagccttct tcatcttctc
     2761 agtcatacga agtgcgtctt cctcattgat cgtctgctct gctttctcga tcagtgacaa
     2821 tacatctccc ataccaagga ttcggcttgt cattcggtct ggatagaact gctgaagatc
     2881 ttccagtttt tctcccatac ctacatataa aatcggcttt cctgttacag aacgaataga
     2941 aagtgctgca ccacctcggg tgtcaccatc catcttcgtg atgataattc catcgacgcc
     3001 gactttctca ttaaattctt ttgccacatt gacagcatcc tgtcctgtca tggcatccac
     3061 aaccagaata ctctggaata catccacatt ctctttgatc tcaaccagct ctgtcatcat
     3121 attctcatca atatgaagac gtcctgctgt atctaggatc acgatattgt ttccattctt
     3181 ctgtgcgtgt tctacggaag cctttgcaat atccactgga ttctgcttat ctcccattgt
     3241 aaatactggt actccgacct tctctccatt gacctgaagc tgtttgatcg ccgctgggcg
     3301 atatacatca caggctacca ataatggtcg ttttcctttc ttcttatact gtccggcaag
     3361 ttttgcacat gttgttgtct taccagcacc ttgaagacct gccatcaaaa taatcgtaat
     3421 ttcattacct ggacgaagct ggatctctgt cattgttgat cccatcaacg cttccatctc
     3481 ttcttttacg atcttgatca ccatctgtcc cggattcaaa ccattcagaa catcttgtcc
     3541 tacagctctt tcctgtacag atttgataaa tgtctttaca actttgaagt taacatcagc
     3601 ttcaagtaaa gctcttttaa cttctctcat tgccgctttt acatcatctt ctgttaaacg
     3661 gcccttactt cttaagttct taaatacacc ctgaagtttc tcggataaac tttcaaatgc
     3721 cattgattca cctcatctaa gttataaatc ttctaaaacc tgattacata cagattctat
     3781 ttgtctgata tcttcttcca tctgctcttt atcttcttta agaagccttc tggataaggt
     3841 atggatctct ttgatcttct ccttggccgt caaaaacttc gctaccagat gaagtcttgc
     3901 ttcataatct gccaagatct tatcacatct tcggatcata tcatgcaccg cctgtctggt
     3961 aattccaaac tcagctgcga tctctgtggc tgtcatatca ttcaaaatat gttcaccata
     4021 tatctccttc tgatgtgctg tcagcaattc gccgtaaaaa tcatataata aattctgttc
     4081 tacgattcgt tccatctgat actcctgtaa agcaattttc ctttaccaga tgttagttta
     4141 gcgaatttca ctgcctttgt caagtatttt tacttgcaca atgcaaattt tacttgaaaa
     4201 actctagctt ttctgtaaaa tgaaaagagc attctattta attataaatc aaaaagaaag
     4261 gttatacaat gattcaggca aataatgtaa ctttacgttt aggaaaacgt gctttattcg
     4321 aagatgtaaa tattaaattc acagaaggta actgctatgg actgatcggt gccaatggag
     4381 caggtaaatc cactttctta aaaattcttt ccggagagat cgaaccttca aaaggagatg
     4441 tatccattac ttctggacag cgtatgatga tcatatcgtc cttgatacgg ttatgatggg
     4501 taaccaaaga ttatacgaga tcatgaaaga aaaagaagct ttatatgcga aggaagactt
     4561 cacagacgaa gacggcatcc gtgctagtga attagaaggc gaatttgctg atatgaatgg
     4621 atgggaagca gaatctgatg ctgctacctt attaaatggt cttggaattc ctagcgatat
     4681 ccattataca aagatggctg acttaaacgg ttctcagaaa gtcaaagtct tacttgcaca
     4741 ggctttattc ggaaatccag atatcctgct gcttgacgag cctaccaacc acttagactt
     4801 agacgctatt tactggttag aagaattcct gatcaacttt gacaacacgg tcatcgttgt
     4861 atcccatgac cgttatttct taaataaggt atgtactcat atcgcagata tcgattatgc
     4921 gaaaattcag ttattctctg gaaactatga tttctggtat gaatccagcc agcttcttac
     4981 aaaacagatg aaagaagcga ataagaaaaa agaagaaaag atgaaggaat tacaggactt
     5041 tatcgcaaga ttcagtgcca atgcttccaa atccaaacag gctacttcaa gaaagaaagc
     5101 tttagataag atccagctag atgagttaaa accatccagc cgtaaatacc catatatcaa
     5161 cttcaagcca aaccgcgaaa tcggaaatga tgctttagtt gttgaaaatc ttacaaagac
     5221 gatcgatggc gtaaaagtct tagataatat ttctttctta atgaaaccaa cggataagat
     5281 tgctttcgta ggtcctaata cattggctgc tacaacatta tttaagatct tatccggaga
     5341 aatggaacct gattctggtt cttacaaatg gggagtgact acaacgcagt cttatttccc
     5401 aaaagataat acaaaagatt tcagtcagga tgaaacgatc gttgaatggc tgactcagta
     5461 ttctgaagat aaagatgcaa catttgtccg tggattcctt ggacgtatgt tattctctgg
     5521 agaagacgca ttaaagaaag ttggtgtttt atctggagga gagaaagttc gttgtatgct
     5581 atctaaatta atgatcagcg gagccaacat cttacttctt gatgagccta ccaaccactt
     5641 agatatggaa tctatcactg ctttaaatga agcattaaaa gatttcactg gtgcattatt
     5701 gtttacttct caggatcatc agtttgtaca gacaacagct aatcgtatca tggaaatcac
     5761 tccgaatggt ctgattgaca aagaatgtac ttacgacgaa tatctggaaa atgacgaagc
     5821 tgccagaaaa cgtcagattg ttgagattga agaagacgac gaataatttc atagttctaa
     5881 acaaaaacac agagatttta tcaccaatct ctgtgttttt attcttattt aattttcctg
     5941 atcgtaagta tctaagaacg aatgttttac tccatctttc ttatatgcaa tgatcgtctt
     6001 taaattgaca ttttctacac tcttgaaaat attggactct acatacggat tgatctcttt
     6061 catcttcgcg atcaactgtt ttgtctccat tctcttggat ttcgtaacac cgtctggatt
     6121 acatgttgta caagtatctg taaacttaca tgcacactgg ataaaatgta atccattgta
     6181 atctctccaa tgctttatat catcctctcg gatcagatac atcggacgga ttagttccat
     6241 tccttcaaag tttgtactgt gaagttttgg catcatcgtc tgtacctgtg ctccataaag
     6301 cattcccata aggatcgtct caatgacatc atcatagtga tgtcccaacg caatcttatt
     6361 gcatccaagc tctttagcct tgctgtataa atatccacga cgcattcttg cacacagata
     6421 acatggcgat ttctcaatct cataaacagc atcaaaaata tcagattcaa agactgtgat
     6481 cggaatatct aataatttgg cattattctc tatgatctgt cgattcattt cattatatcc
     6541 tggatccatg actaaaaata caagatcaaa atggaatttg ttatggcgtt ttaattcctg
     6601 aaataatttc gccatcaaca tggaatcttt tcccccagag atacagatag cgatcttatc
     6661 attctcctgc actagatcat atgtcttgat cgcttttaca aacttggaga atatctgttt
     6721 gtgaaacttc ttacggatgc tcttttcaat ctcctgtgtt cttgtcagtt cttttttctt
     6781 gccttttcct tcggcttctt tgttattttg ttctgttttc tgatcgtttt cctgttcttc
     6841 tttcatcaag gataacaacc atcctgtgta tccgccttcc aaactaagga cattgattcc
     6901 ttcttccgct aatttctctg ctgcctcttt gctgaaaatt cccttggtac aatataaaat
     6961 aactttctta ccctgatcaa tatattcctt tgcctgtgtt ccaagctcat cttcacctgc
     7021 tgccacaaca gctccaggga tcattccata agatgtgttt gattcatctc tcatatcaag
     7081 aagctgataa ctctcttttt ctaactgttt taattcttta tatgaaatat taatattgct
     7141 catgctttca acctttcttt cgtatcctac tatatttgta gggttccgtt tatttattat
     7201 agagagaaag ctttcaaaat acaagagcaa atctttattt tgcatcagtt gttgtataca
     7261 ttgttgatca taaatccata accactccat tcgattggaa tgtatgtctg ttttttattt
     7321 tgtatcgcat aaatacctat tctgcccgaa atctgagaat aagatccctt tgctgacttt
     7381 gctttttggt attgttctgt tacattgaca ttgcccatca aacgcttctt caaattatac
     7441 tctgactgtt tcagattcag ataagacaaa aatccagccg gaatatcttc caaatgatct
     7501 gcgatatctt tccaatcttt tgtgtaatca taagagtaca catcaatcgc taaaatctga
     7561 tccgtatacg catccataat aagattcact atttcatcct ccgacgattc tggtgataat
     7621 gtaatatacc ataccagaag atttttgaca ttcgaaatat tagatacgga accatcatcg
     7681 atcatatcat cctttttctt gatataagtg tataattttt tatatgtaat cttccagtct
     7741 tcctctaccc gataagcgat tttcattttt tttaataata gattcgtctg tttttttgca
     7801 gttttttcta catctcttcc atccacagtt cttgatgaag gtcttactgc tacactcaaa
     7861 atatgattat tgatgatctt taatttttcc agagttgtct gagtagcctc taccgaaatc
     7921 tctactggat ttaaggatag atcctcactc tgcccaaacg tttttttgtc caccagagct
     7981 gcctgaatat ttggtaaaaa gtaaaacaag accacaagta ataaagccgc cacaacaaga
     8041 agagcattcc taatttgttt tttcataagc tcctcctcca attgttatga tcacagttgt
     8101 tcctttccct tgctgactga taaatcgcat ctctccttga tggatcctca cgatttcttt
     8161 acatatagca agccctaaac ctactccacc ttgttttctt gaccttgact tatctatccg
     8221 gtaaaacgct tccgtgatct tacttatctc atcctttggc attccgcatc cagtatccga
     8281 aatataaatc ttggctcctt cttgtattgc tcgatcctca actgttaata ttccacccga
     8341 attcattgat tttaatgcat tatcgatcaa atttaatatt aatgatttca ataaatccgg
     8401 ctctatctta caaattgctt tttttaaatt taaattcaca agaatatctt gttcttttaa
     8461 acgatactgc atggatgtaa ctgcttcttg aatcattggc tctaaatctt gttttttcat
     8521 ctgaaactgt tgatttttca aaacaagaag tttcattaat tttaatgata aattctgtaa
     8581 tcgttttcct tctgaataaa tatacccaca tgcttctttt tgctcttctt tcgaaagagc
     8641 ttccatccgc atcagatctg cataccctat gatcgatgta agtggtgtct tcatttcatg
     8701 agcaaaactt cccataaatt cttcctggtt ttttaacaat tgatccaatt tcatta
//
LOCUS       DAOMUE010000050         8593 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2109_length_8593_cov_2.886507, whole genome shotgun sequence.
ACCESSION   DAOMUE010000050 DAOMUE010000000
VERSION     DAOMUE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8593
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2109_length_8593_cov_2.886507"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PLC71_03770"
     CDS             complement(<1..1166)
                     /locus_tag="PLC71_03770"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HRM01794.1"
                     /translation="MKKVYVIGHRNPDTDSICSAIAYANLKNQVEGNGYEAYRAGNIN
                     GETRYVLDTFGIKDPKYVSDVRPRLKDVNLNLVKGAKATDSLKSAREMMQTEKIVSLP
                     VLDGKKMIGLVSISDVLKADMDVYDNEILAKAKTPYKNVVETLDGTLQAGDIEGYITE
                     GKLTVSAASVDTMEEFVTAKDTVIVANREDAQIGAIQTGVQCVVVCMGTEVTDKVLEL
                     AKEKNCRIITTPYDTFTASRLICQAMPVSYIMATDTIVSFNETDFVDDVKEEMTKKRF
                     RYFPVLNADNEFVGFISRRRILEFNKKQVILVDHNEVSQAVEGLEECEIMEIIDHHRI
                     GSVETVSPVYFRNQPVGCTGTIVAQMYREQGIEITKEMAGLMCSAILSDTLMFRS"
     gene            complement(1163..2296)
                     /locus_tag="PLC71_03775"
     CDS             complement(1163..2296)
                     /locus_tag="PLC71_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM01795.1"
                     /translation="MRSKYRSLKIRIILQTLGISLIVACIGYFVLDIFVDGVFQDPFA
                     KTVVYIFERIGMDELEAIRVYRLIFVQNKTMIIGAGFFVLLLLSFYLGMSKFTTYLRQ
                     VEGGIQMILNESKDRILLPPELNPLENKLNEIKGTLKEQRRIAEESEQRKNDIIVYLA
                     HDLKTPLTSIIAYLSLLNESPDMSEEQRAKYTGISLEKAKRLGELINEFFEITRYNLQ
                     NITLDKKEFYLYVLLEQVMDSFAAVFKQKGLEWKIEVDDKILIYGDPDRLARVFENLL
                     RNAVSYCYPNSLIEVYGMIDGDNAEILVRNQGKTIPEHQLNNLFEKFYRLDEARSSET
                     GGAGLGLAIAKEIIELHDGTIKAKSQNEYTTFIIRLPRKKEEQ"
     gene            complement(2286..2987)
                     /gene="vanR"
                     /locus_tag="PLC71_03780"
     CDS             complement(2286..2987)
                     /gene="vanR"
                     /locus_tag="PLC71_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanR-ABDEGLN family response regulator
                     transcription factor"
                     /protein_id="HRM01796.1"
                     /translation="MRQSTVLVVDDEKEIADFIELYLKNENYNVHKFYNGKDALACVE
                     HENIDLAILDVMLPDIDGFEILKQIRLTHNFPVIMLTAKVSSMDKINGLTLGADDYIE
                     KPFEPLELMARVKAQLRRFTRYNDGSGMNQEDTLEIAGLILNRATHQCTYGEEEITLT
                     PLEFSILWILTQNRGKVISSENLFEQVWKEKYFKNNNNTVMVHIRHLREKLGEVMGHK
                     ELIKTVWGVGYKVEE"
     gene            complement(2999..4027)
                     /locus_tag="PLC71_03785"
     CDS             complement(2999..4027)
                     /locus_tag="PLC71_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01797.1"
                     /translation="MARIEESKRTYIEELATNPRVNLMVLSERIDIVFHEDESEVSLA
                     EKIAEKLYDMPQLITKLLQQEAIEFLLQCWDMEGESLIAEMYAREIEQLHFLGFLSYE
                     DDTILLNIEAKDNFFFSLKSRRVQEELEEGTRLENILFGMLFLYGILDIYECCQMIQE
                     EMFPELTYDELEEFILLRIVFWQSGILLRNQMNSRLLLASREVENRNEVFIQWSLRED
                     LSWKRYSEDEYKNLALGNGIGGWDGIPELYDFVMKNIENDQYKVMMIVKSLVVKIQNG
                     LTYEEITSAYVNLLDEEDKEHQRILCELIRTLYKTVPLYSLKGHTRTEVEEESQEARF
                     HVIQGGKE"
     gene            complement(4112..4945)
                     /locus_tag="PLC71_03790"
     CDS             complement(4112..4945)
                     /locus_tag="PLC71_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HRM01798.1"
                     /translation="MPGIILEGGTFRPIFSAGVMDALLDEDIMFPYMIGVSAGICDGF
                     SYISKQKERNLKILMNHRNDKRYMGAGNLLKERSLFGLDFVYDTIPNKLYPFDWKTFN
                     EYKGTIKVGVTNAWTGKAEYKDGMKLDKKCTMLRATCAIPFAFPTIKVDGKPYYDGGI
                     ADPIPIRRSIEDGNEKNLIVLTRPEGYRKELSKSNKAAAKLLKRKYPHLPELLLNRHI
                     RYNKTVRYCEQLEREGKAVILRPEYTIDSLEKDIDVLKQTYDMGYRVTKENINRIKEL
                     F"
     gene            complement(5127..8075)
                     /locus_tag="PLC71_03795"
     CDS             complement(5127..8075)
                     /locus_tag="PLC71_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM01799.1"
                     /translation="MDVKNHNMNKEKPIMRKRLKVIAFLAFFLGIVLAVSQYFGFVSK
                     TVYEESVSHLTEVFHQSDNMLGELTHKNLMYLHMWSEYLQDAPSESKIRDYIDKAQKD
                     AGFLYFYFLSADGNYKMTTGETGYLGLQENIEDEIQKGNDIITNAAVPGKSQMLVFAS
                     PKAHGSYQGFKYDAIAIAYENADIVNVLDISAFNGKAKSYVLHPDGRVVIDHSLESWG
                     NVYNFFGILREHTDISEKEILEISKKFKEGHTGAMLLNLDEKDYYLVYEKSGIQDWVF
                     LGLVQADIVNASMNTLQLSTMLLVGAVVLCITAVLIGFILRKNSVRLKKKDTEILYRD
                     EMFQKLSMNVDDVFLMLDAKTYQADYVSPNVEKLLGITIEQIREDICILGELHSEDTK
                     DPKKDHLKEIQVHEQQEWDFEYVHQKTGERRWFHSVAMGSEVNGNKKYILVMSDRTAD
                     KKMNQALSDAVHAAENANQAKSTFLSNMSHDIRTPMNAIIGFTTLAVSNINNKKMVRD
                     YLGKILSSSNHLLSLINDILDMSRIESGKIHLEETEVSLSDVLHDLKTIISGQIHAKQ
                     LELYMDAMDVMNEDVYCDKTRLNQILLNLLSNAIKFTPAGGMISVRLKQYPGTQRERQ
                     LYEIRVKDNGIGMSEDFVQKLFSPFERERSSTVSRTQGTGLGMAITKNIVDMMGGNIE
                     IQTEQGKGTEFIVRLPLRTQSKQHRVEKIAALEGLKALVIDDDFNTCDSVTKMLAKVG
                     MRSEWTLSGKEAVLRARQSIELGDAFHAYIIDWRLPDMNGIEVTRQIRSLGDDTPIII
                     LTAYDWSEIEAEARAAGVNAFCAKPIFMSDIRDTLMTAIGQKQDRTNDDILPAVSSDF
                     RGRSILLVEDNELNSEIAIAILNEYGFQVHTAENGAEAVEKIRNSAPGDYELVLMDIQ
                     MPVMNGYEAAKQIRALDDPALAEITILAMTANAFDEDRKKALECGMDGFLSKPIVIEE
                     LIHTLQTNLK"
     gene            complement(8081..>8593)
                     /locus_tag="PLC71_03800"
     CDS             complement(8081..>8593)
                     /locus_tag="PLC71_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM01800.1"
                     /translation="CFLLRSAHTLRGVYGTDVLIATGNSFTGSVRKAGYTEKMAGNMI
                     MPNDLSAYKRKMSGAELKKTVRNFVEGYQGGFNPFNRGSLPVVSGISVEIKETEDGYT
                     LSKVTKNGKSVQDKDTFTVTCLATPQHMESYPAGKNIVFDGEVKDTWTKCVLKGDAVL
                     AKPEDYMTLR"
BASE COUNT         2341 a         2057 c         1302 g         2893 t
ORIGIN      
        1 gaacggaaca ttaatgtatc agaaagaatt gctgaacaca ttaatcctgc catctctttt
       61 gtaatctcga ttccctgttc acggtacatc tgagcaacga tcgttcctgt acatccaact
      121 ggctgatttc tgaagtaaac tggtgataca gtttccacac ttccgattct gtggtggtcg
      181 atgatctcca tgatctcaca ttcttctaat ccttcgactg cctgtgatac ctcattatgg
      241 tcaactaaga ttacctgttt cttgttgaat tctaagatac gacgtctgga aataaatcct
      301 acaaattcgt tatctgcatt caatacaggg aagtaacgga aacgtttctt tgtcatctct
      361 tctttgacat catctacaaa atctgtttca ttgaaagata cgatcgtatc agttgccatg
      421 atataagata ctggcattgc ctggcagatc aaacggcttg ctgtaaatgt atcatatggt
      481 gttgtgatga ttctgcagtt cttttccttt gcaagttcta atactttatc agtaacttct
      541 gtacccatgc atactacaac acactgtact cctgtctgga tcgcaccaat ctgtgcatct
      601 tcacggtttg caacgatcac tgtatcttta gctgttacaa attcttccat tgtatcaaca
      661 cttgcggcag atactgttaa cttaccttct gtgatatatc cttcgatatc tcctgcctga
      721 agtgttccat ctaatgtttc tacaacattt ttatatggtg tttttgcttt ggcaaggatt
      781 tcattgtcat aaacatccat gtctgctttc aacacatcac tgatggatac cagaccgatc
      841 attttctttc catctaatac tggaagagac acgatcttct ctgtctgcat catttcacgt
      901 gcagatttca gggaatctgt cgcttttgca cctttaacta gatttaaatt tacgtctttt
      961 aatcttggtc ttacatcact aacatatttt ggatctttaa ttccaaatgt atccaataca
     1021 tatcttgttt cgccgttgat attaccggca cgatatgctt cgtatccatt tccttccact
     1081 tgattcttta agtttgcgta tgcgatcgca gaacagatgg aatctgtatc tggattgcga
     1141 tgtccaatga catatacttt tttcattgtt cctccttctt tcttggcagc cgtattatga
     1201 atgtcgtata ctcattctgg ctttttgctt tgatagttcc atcatgaagc tctatgatct
     1261 ccttcgcgat cgcaagtcca agacctgccc cgccagtttc agaagatctt gcttcatcta
     1321 atctgtaaaa tttctcaaac aggttattta attgatgttc tggtatagtc tttccctgat
     1381 tcctgactaa aatctctgcg ttatctccat cgatcattcc ataaacctcg atgagagaat
     1441 tcggataaca atacgatact gcattcctta acagattctc aaaaaccctg gcaagcctgt
     1501 ccggatcacc ataaattaat attttatcat ctacctctat cttccactct aatcctttct
     1561 gcttaaatac cgcagcaaaa gaatccatca cctgttcaag cagaacataa agataaaatt
     1621 cctttttgtc caaggtaata ttctgaagat tataccttgt aatttcaaag aactcattga
     1681 tcagttctcc aagacgtttc gctttctcaa gagaaattcc tgtatactta gctctttgtt
     1741 cttccgacat atccggcgat tcatttaaca atgataaata tgcaatgatc gacgtcaacg
     1801 gtgtcttcag atcatgagca agatagacga tgatatcatt ctttctctgc tcactttctt
     1861 ctgcaatccg cctctgttct ttcaacgttc ctttgatctc atttaattta ttctccaaag
     1921 gattcaactc tggtgggagc aatatgcggt ctttggattc atttaagatc atctggatcc
     1981 cgccttcaac ctgtcttagg tatgtcgtga attttgacat acccagataa aagcttaaca
     2041 aaagcagcac aaaaaatcca gcaccaatga tcattgtttt attttgtaca aagatcagac
     2101 gatatacacg gatcgcttcc agctcatcca tcccgatccg ttcaaatata taaacgaccg
     2161 ttttggcaaa tggatcctga aatacaccat ctacaaatat atcaagtaca aaatatccga
     2221 tacatgcaac gatcaaactg attcccaatg tctgtagtat gatccttatc tttaaacttc
     2281 tgtatttact cctcaacttt gtatccaact ccccagactg tcttgatcaa ttctttgtgt
     2341 cccatgacct ctcccagctt ctcacggaga tgtctgatat gtaccataac tgtattattg
     2401 ttgttcttaa aatacttttc tttccatacc tgctcaaaaa gattctctga gcttataacc
     2461 ttcccgcggt tctgtgtcag aatccataaa attgaaaatt ctagcggtgt caatgtaatt
     2521 tcttcttctc cataagtaca ctggtgtgtt gcacgattta agataagacc tgcgatctcc
     2581 agtgtatctt cctgattcat accgcttcca tcgttgtatc ttgtaaatct tcgaagctgt
     2641 gccttcactc ttgccatcaa ttccaacggc tcaaatggtt tctcaatata atcatcagct
     2701 cccaatgtca atccattgat cttatccatg gaagatactt tcgctgtcag catgatcaca
     2761 gggaaattgt gtgttaaacg gatctgtttt aaaatctcaa atccatcaat atctggcagc
     2821 atcacatcaa ggatcgccag atcaatattc tcatgttcta cacacgccaa ggcatccttt
     2881 ccattgtaaa atttatgtac attataattt tcattcttta aatataattc aataaagtca
     2941 gcaatctctt tctcgtcatc tactactaat acagttgact gtctcattcg cttcctcctt
     3001 actctttacc accctgaatc acatgaaatc ttgcttcttg gctttcttct tccacttctg
     3061 ttctcgtatg tcccttgaga ctatataatg gtacagtttt ataaagcgta cggatcaatt
     3121 cacaaaggat cctttgatgt tctttatctt cttcgtccag aagattcaca tacgcagatg
     3181 taatctcttc ataagtcaat ccattttgaa tcttaacgac cagtgatttt acgatcatca
     3241 taaccttata ctgatcattc tcgatattct tcatcacaaa atcatataat tctgggattc
     3301 catcccagcc tccaattcca ttgcctagtg ccagattctt atattcatct tcactgtaac
     3361 gtttccatga tagatcttcc ctaaggctcc actgaataaa tacttcgtta cgattttcta
     3421 cctcacggct tgccagtaac agtcttgaat tcatctgatt tcttaataag attcctgact
     3481 gccagaatac aatacggagt aatataaatt cttccaactc atcataagtc agctctggga
     3541 acatctcttc ctgaatcatc tggcaacact cataaatatc taaaatacca tataaaaaca
     3601 acattccaaa gagaatattc tcaagtctgg taccctcttc taactcttcc tgaactctgc
     3661 ggcttttcag agagaagaaa aaattatcct ttgcttctat attcaataag atcgtatcgt
     3721 cttcatatga gagaaatcca agaaaatgta actgctcaat ctctcttgcg tacatctcag
     3781 caatcagact ctcaccttcc atatcccagc attgtagcaa gaattcgatt gcttcctgct
     3841 gtaataactt agtgatcaat tgtggcatat cataaagttt ctctgctatc ttctctgcca
     3901 gagatacttc actttcgtct tcatgaaaca caatatcgat tcgttccgat aaaaccatca
     3961 ggttgactct tggattggtc gcaagttctt ctatataagt tcgttttgat tcttcaattc
     4021 ttgccatcgc ccgatccctc ctttgtctta atcttataaa gtatatctta tcgagcttct
     4081 tccgtccagt ggaacaggca taaagagtcg tctaaaataa ttctttaatt cggtttatat
     4141 tctcttttgt gacacggtat cccatatcat acgtttgttt taatacatca atgtctttct
     4201 ccagactatc gatcgtatat tccggtctta ggatcactgc ctttccttcc ctctctaact
     4261 gctcacaata cctaactgtt ttgttatatc ttatatgacg atttaatagc agttccggta
     4321 aatgtggata ttttctcttg agaagttttg ccgcagcttt gttgctcttg cttaattctt
     4381 ttctataccc ttctggcctt gtcagtacga tcagattctt ctcattccca tcttctattg
     4441 atcgtctgat cggaatcgga tctgcaattc caccatcata ataaggcttt ccatctactt
     4501 tgatcgttgg aaatgcaaac gggatcgcac aagttgctcg aagcatcgta cacttcttat
     4561 ccagtttcat tccatctttg tattctgctt tccctgtcca tgcatttgtt acaccgacct
     4621 tgatcgttcc tttgtattca ttgaatgtct tccagtcaaa tggatacagt ttgtttggga
     4681 tcgtgtcata tacgaaatcc agtccaaaaa gacttctctc tttcagtaga ttccccgctc
     4741 ccatgtatct tttatcattc ctgtggttca tcaggatctt taaatttctt tccttttgtt
     4801 tggatatata agaaaaacca tcacagattc ccgcagatac tccgatcata taaggaaaca
     4861 tgatatcttc atccaacagt gcatccatca ctcctgcact aaagatcgga cgaaatgttc
     4921 ctccttctaa aataatacca ggcatcttta tgccctcctc atcttcttat atttcgtaaa
     4981 cttgtcttat gttgtgtcat gtgagttcta tagacacttg ctctttatta tatcacgttt
     5041 cgattctcca ttgttacatt ttgtcaaaga ttttcttaag agatttcgaa atttttgtga
     5101 ataaaaaaga tatcgcttcc tttcatttat ttcaaattgg tttgtaacgt atggatcaat
     5161 tcctcaataa caattggttt ggataaaaat ccatccatac cacattccag tgctttcttt
     5221 ctatcttcat caaaagcatt ggcagtcata gcaaggatcg taatttctgc cagtgctgga
     5281 tcatccagtg cacggatctg ctttgcagcc tcatatccat tcattacagg catctgaata
     5341 tccataagta caagctcata atcgcctggt gctgaattcc tgattttctc cactgcctct
     5401 gctccatttt ctgctgtatg aacctgaaat ccatattcat taagaatcgc aatcgcaatc
     5461 tcactgttta attcattatc ttctacaagc agaatacttc tgcctctgaa atctgaactt
     5521 actgccggaa gaatatcatc attcgtccta tcctgcttct gaccgatcgc tgtcatcaaa
     5581 gtatctctga tgtctgacat gaatataggt ttcgcacaaa atgcattgac tcctgctgct
     5641 cttgcttccg cttcaatctc tgaccagtcg taagcagtca ggataatgat cggtgtatca
     5701 tcaccaagac tacggatctg tcgggtaact tcaataccat tcatatctgg caagcgccaa
     5761 tcgatgatat aggcatggaa tgcatcacca agctctatag actgccttgc acggagaacc
     5821 gcctccttac cagaaagtgt ccattccgaa cgcattccaa cctttgcaag catctttgtc
     5881 acgctgtcac aggtattaaa atcatcatct atcaccaatg ccttaagacc ttccaacgct
     5941 gcgatcttct ctacacgatg ctgcttagac tgggtccgca acggcagtcg tacaataaat
     6001 tccgtacctt tgccctgttc tgtctggatc tcaatatttc cgcccatcat atccacgata
     6061 ttcttggtaa tagccatgcc cagtcctgta ccttgagtcc tgctgactgt agaacttctc
     6121 tcccgctcaa aaggagaaaa aagtttctgt acaaaatcct cactcatacc gattccgtta
     6181 tcctttaccc gaatctcata taactgacgc tctcgctgtg ttcccggata ttgtttcaac
     6241 cgaacagaaa tcattccgcc tgcaggagta aacttgatcg cattggacaa aagattcagc
     6301 agtatctggt ttagccgtgt cttatcgcaa tacacatcct cattcataac atccatggca
     6361 tccatataaa gttccagctg cttcgcatga atctgtccac tgatgatcgt cttcaggtca
     6421 tgaagtacat ccgacaaact aacctctgtt tcttccagat ggatctttcc actctcaata
     6481 cgactcatat ccagtatatc attgatcagt gaaagcagat gattgctgga tgaaagaatc
     6541 ttgccaagat aatctctgac cattttcttg ttattaatgt tgctcacagc cagtgtcgta
     6601 aacccaatga tagcgttcat tggcgtccgg atatcatgag acatgttaga aagaaaagta
     6661 ctctttgcct ggttggcatt ctccgcagca tggactgcat cagaaagtgc ctgattcatt
     6721 ttcttgtcag cagtacgatc cgacataacc agtatatatt tcttattccc atttacctca
     6781 ctacccattg caacactatg aaaccagcgt cgttctcctg ttttctgatg aacatactca
     6841 aaatcccatt cctgctgttc atgaacctga atctccttta gatgatcctt cttcggatct
     6901 ttcgtatcct cagaatgcag ttctcctaaa atacaaatat cttcccggat ctgttctatg
     6961 gtaattccca acagtttttc cacatttgga ctaacataat ctgcctgata cgtttttgca
     7021 tccaacatta agaatacgtc atccacattc attgacaact tttgaaacat ctcatccctg
     7081 tacaagatct ctgtgtcttt tttcttcagt cttacactgt tttttcgtaa aataaatcca
     7141 ataaggactg ctgtaataca aagcacgact gcacccacaa gaagcatcgt actaagttgc
     7201 aaagtgttca tactggcatt gacgatatct gcctggacaa gtcccagaaa tacccagtcc
     7261 tgaatgcctg atttttcata aacaagataa taatcctttt catccagatt tagtaacatc
     7321 gcacctgtat gtccctcttt aaatttcttc gaaatttcaa ggatctcttt ttcggagata
     7381 tctgtatgtt ctctcagaat gccaaaaaag ttatatacat ttccccatga ctctaaagaa
     7441 tgatcgatca caacacgacc atctggatga agaacataac tctttgcttt tccattaaac
     7501 gcagagatat cgagtacatt tactatatca gcgttttcat aggcaatagc aattgcatca
     7561 tatttaaatc cctgataact tccatgagcc ttcgggctgg caaaaacaag catctgggat
     7621 tttccgggaa ctgctgcatt ggttatgata tcattgcctt tttgaatctc atcttcaata
     7681 ttttcctgta atccaagata ccctgtttca cctgttgtca tcttatagtt accgtcagct
     7741 gatagaaaat aaaaatataa aaatcccgca tctttctgcg ctttatctat ataatcacga
     7801 attttgcttt cacttggagc atcctgcaga tactcactcc acatatgaag atacatcaga
     7861 ttcttatgtg tcagttcacc taacatatta tccgactgat ggaaaacttc tgttagatga
     7921 gaaacacttt cctcatatac agttttcgat acaaatccaa aatattgaga aacagccaaa
     7981 acgattccaa gaaagaatgc aagaaaagca atgaccttga gtcgttttct catgataggt
     8041 ttctctttgt tcatattatg attcttaaca tccattcttc tcacctcaga gtcatatagt
     8101 cttcaggctt tgcgagaacg gcatcaccct tcaaaacaca ttttgtccag gtatctttca
     8161 cctctccatc aaatacaatg tttttacccg ctggatacga ttccatatgt tgtggtgtcg
     8221 caagacaggt tacagtaaag gtatccttat cctgaactga ttttccattc tttgtaactt
     8281 tacttaatgt ataaccatcc tccgtttcct tgatctctac agaaatacca ctgaccacag
     8341 gcagagagcc acggttaaat ggattgaaac ctccctgata gccttctaca aaatttctga
     8401 ctgtcttttt cagctcagcc ccactcatct tccgtttgta agctgaaaga tcatttggca
     8461 tgatcatatt gccagccatt ttttccgtat atcctgcctt ccgtacactt cctgtaaagc
     8521 tgtttcctgt tgcgatcagc acatccgtac cgtagacgcc acgcaaagta tgtgctgatc
     8581 gcaacaggaa aca
//
LOCUS       DAOMUE010000051         8579 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2114_length_8579_cov_2.788128, whole genome shotgun sequence.
ACCESSION   DAOMUE010000051 DAOMUE010000000
VERSION     DAOMUE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8579
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2114_length_8579_cov_2.788128"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="PLC71_03805"
     CDS             complement(<1..138)
                     /locus_tag="PLC71_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRM01801.1"
                     /translation="MTMSDQYDVIMIGAGPSAIFCAYELKRNKPDMKVLMIEKGRRIE
                     KR"
     gene            complement(135..647)
                     /locus_tag="PLC71_03810"
     CDS             complement(135..647)
                     /locus_tag="PLC71_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01802.1"
                     /translation="MKHITINRAEQADVDAIMQLMQEAVDTVKVSDWFLPDNRQYIEE
                     HISDRGFTLKALSSNNEILGFFIVDLPGESKHNLGYDLNYDDHKIHKVAHMDYAVVTS
                     KARGLGLQRRFFKEAEKQLEDTIYEYFLGTVHPDNIYSRTNFVKAGYREARQMSKYGG
                     MQRIIMEKEK"
     gene            796..2271
                     /locus_tag="PLC71_03815"
     CDS             796..2271
                     /locus_tag="PLC71_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRM01803.1"
                     /translation="MINRLCKRLNQNIEVRQSLSSLRQEIKDSSKRELLWSWIHDGDL
                     DLSVFLENEDAKTRKNAALLIGDLALSSESDAVFHAYQTEDTRFVKEAYLTALKSLNA
                     APYVDVFRKRYEELSLYEASDDEKKHVEHELHALSELINTIEPFKKHRFLGGRQTFHC
                     IFRTNPLHPEITAALMEESSAASSKMGVRVKTNHLNRLLPIRTYNELLFQIPGMVSCK
                     PDADVAASVIAGSNLMALLENTHEGDFPFYFRIGVKSRMALSERSKFAKKVASKLEEL
                     TAHKLRNSTSHYEFEIRMIEGKSGDYYLLVKLNTIVDRRFSYREEFIPTSIKPVNAAL
                     LVELAKDYMIPDAQILDPFCGVGTMLIERQKVVKGNTSYGIDHSPEAIEKAIYNTNLA
                     DQIVHYINKDCFTFTHDYPFDEIFTEMPYATGQKTEAEIREVYEKFFPFAKRVLGPEG
                     TIIMYTRNREYVKQFAVKSNFRILKEIKITQRPESYLMILK"
     gene            complement(2342..3283)
                     /locus_tag="PLC71_03820"
     CDS             complement(2342..3283)
                     /locus_tag="PLC71_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM01804.1"
                     /translation="MNNKQKLLTATAITGTIVSASAFTVFDYAICRRKKQPDVRKKEK
                     WKPYLDYVLKEQEWLKEHQEQELKEIRSKDGLTLRAAYIPRENAKGTIICMHGYHSTN
                     DIEFVPEVRFLWNLGYSILLPWQRSHGKSEGRYITYGVKERHDLKRWILYTNRHLAAK
                     NKDIFLCGISMGCATTLMAAGLDLPDNVKGIIADCGFTSPWDIIKHVAKERFHLPPFP
                     LMYMVDLISEVVAGFGLKEVSIPEIMKRNKIPVLFIHGDADDYVPMWMTIKNYEACAA
                     KKELYIVSGAGHALAFSKDMEEGKRRIKNFINKYKTI"
     gene            complement(3314..3742)
                     /locus_tag="PLC71_03825"
     CDS             complement(3314..3742)
                     /locus_tag="PLC71_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HRM01805.1"
                     /translation="MKILVSACLLGIDCKYNGKNNKNDKIIELLKDHELIPVCPEIMG
                     GLPTPRIPAEIHQNKVIAKDGKDVTKQYQKGAEETLKIAKLYNCHTAILKEKSPSCGC
                     GKVYDGTFTGTLINGDGITARLLKEHGIKITGETTLDITE"
     gene            complement(3758..4189)
                     /locus_tag="PLC71_03830"
     CDS             complement(3758..4189)
                     /locus_tag="PLC71_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM01806.1"
                     /translation="MKIKNATIKDFDTAYEYIKKLWDYNTYDYETTKEVYEKVLQDEN
                     SFAFFAIEDDGTFHGFCHGDYFQTFWMSGLTCYVSSLITNEEDRGKGYGVKLLDHAKK
                     LAKERGCKAITLDSGLPRVQAHGFYEHYGFEKSCYGFELAL"
     gene            complement(4318..6048)
                     /gene="aspS"
                     /locus_tag="PLC71_03835"
     CDS             complement(4318..6048)
                     /gene="aspS"
                     /locus_tag="PLC71_03835"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRM01807.1"
                     /translation="MSDMYRTRTMDQISEGDIGAQLKIAGWVENIRDHGGVSFIDLRD
                     MYGVMQVVLRDTSLLDHVRKEECLSVEGVIQHRDEETFNPKIPTGTIELEAHKIDILG
                     KVYRDLPFEIATSKEIREDVRLKYRYLDMRNRKVKDNILFRSKVIRFLREKMEDMGFV
                     EVQTPILCASSPEGARDYVVPSRKYKGKFYALPQAPQQYKQLLMVSGIDKYYQIAPCF
                     RDEDARADRSPGEFYQLDFEMAFATQEDVFKIGEEVLTETFKKFAPEGSVITEAPYPI
                     FSYKEAMLQFGSDKPDLRNPLRIIDVTDFFQRCTFKPFHGQTVHAKLSKGQHEKLLKF
                     AQSIGMGGLGYLEVKEELKYKGPIDKFIPDDMKMELAEMAGLQEGDTIFFIAAPEKAA
                     CIYAGQIRNELGSRLDLLEKDAYRFCYINDFPMYEIDEETGKLGFTHNPFSMPQGGLE
                     ALNEKDPLDILAYQYDIVCNGVELSSGAVRNHDMEIMEKAFDIAGYDKETLKDKFGAL
                     YNAFQFGAPPHAGMAPGVDRMIMLLRNEENIREIIAFPMSGTAQDLMCGAPNEITEQQ
                     LREVHIKVRD"
     gene            complement(6483..7421)
                     /locus_tag="PLC71_03840"
     CDS             complement(6483..7421)
                     /locus_tag="PLC71_03840"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HRM01808.1"
                     /translation="MKFYYAPMESITIYLYRNIYEQLFGEIDKYYTPFIMPNGKRTFK
                     TREFQDVLPEHNEGLHIVPQILTKSSEDFIRTAKELKELGYDEVNLNLGCPSRTVVAK
                     QKGSGFLREPEVLDDFLTEIFEALDMKISIKTRIGLRDTEEFEEILSIYNRHPVYELT
                     IHPRLQQEFYKGKPHLDVFEYAVNHSENPLCYNGDIVTKEDYDNITTRFPDVDAVMIG
                     RGLLRNPALVREIKYGQKLTKRELLKMHDTLYEAYQGYLSGDKNVLYKMKEFWNNAAV
                     MFTNHEKYAKKIRKVQTLKNYEQAVNALFSDQDLID"
     gene            7625..8491
                     /locus_tag="PLC71_03845"
     CDS             7625..8491
                     /locus_tag="PLC71_03845"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRM01809.1"
                     /translation="MKEMKPIKEGKVREIYDNGDSLIMVATDRISCFDVILKNDVTKK
                     GTVLTQMSKFWFDLTKDIVPNHMISVDVKDMPEFFQQEKYDGNSMMCKKLTMLPIECI
                     VRGYITGSGWASYQENGTVCGIKLPEGLKESDKLPEPIYTPSTKAEIGDHDENISFER
                     SIEVLEKEFPGKGQEYAEQLRDKTIALYKKCADYALEHGIIIADTKFEFGLDENGNIV
                     IGDEMLTPDSSRFWPADEYEPGHGQPSFDKQFARDWLKSNEHDWKLPQDIVDKTIDKY
                     FQAYEMLTGKDL"
BASE COUNT         2280 a         1666 c         1574 g         3059 t
ORIGIN      
        1 tcttttttca attcttcgac ctttctcgat cataagaact ttcatatctg gcttatttct
       61 ctttaattca taagcacaaa atatagcaga aggtcctgct ccgatcataa taacatcata
      121 ctgatcactc atagtcattt ctccttttcc atgatgatac gttgcatacc accatatttt
      181 gacatttgtc ttgcttccct gtaacctgct ttgacaaagt ttgtccgact gtagatatta
      241 tccggatgaa cggttccaag gaagtattca tatatcgtgt cttctaattg tttctctgcc
      301 tctttgaaaa atcttctctg aagccccaaa cctcttgctt tacttgtcac aactgcgtag
      361 tccatatgtg caactttgtg tatcttatga tcgtcataat tcagatcata tccaagatta
      421 tgttttgact ctcccggaag atcgacaata aaaaagccca agatctcatt gttacttgat
      481 aaagccttca atgtaaatcc ccggtcgctt atgtgttctt ctatatattg tctgttatct
      541 ggcagaaacc aatcggaaac cttcacagta tccactgctt cttgcatcaa ttgcatgatc
      601 gcatcgacat ctgcctgctc tgctctattg atcgttatat gtttcatata cttccttacc
      661 tattattctc ttagttacgt attattatag catgattttc actttttttg caaaaattat
      721 ttcaataagg tatactgttt ttatagtttt taaggacttt tccttataca cattatttag
      781 aaaaggaatg tatatatgat caatcgttta tgtaaaagat taaatcaaaa tatcgaagta
      841 cgtcagtctt taagttccct gcgtcaggag atcaaggatt cttctaaaag agaacttttg
      901 tggtcttgga tccatgatgg tgatctggac ttatccgtat ttttagaaaa tgaagatgca
      961 aaaacaagaa aaaatgctgc tcttttgatc ggtgatctgg cactttcttc tgaaagcgat
     1021 gctgtatttc atgcttatca gacagaagat acacgttttg taaaagaggc ttatttaact
     1081 gctttaaaat ctttaaatgc ggctccttat gtggatgttt tccgaaaacg ttatgaagaa
     1141 ttatctcttt atgaagcttc tgacgatgag aaaaagcatg tagaacatga acttcatgca
     1201 ctttctgaac tgatcaatac gatcgaacca tttaagaagc atagattttt aggcggccgc
     1261 cagacatttc attgtatttt ccgcactaac ccattacacc cggaaattac tgctgccttg
     1321 atggaagaat cttcagctgc ttcttcaaaa atgggcgtgc gtgtaaagac aaatcatctg
     1381 aatcgtttat taccgatccg cacatacaat gaattgttat ttcagattcc aggcatggtt
     1441 tcatgtaaac ctgatgctga tgttgctgca tccgtgatcg ctggttcgaa tttaatggca
     1501 ttattagaaa atactcatga aggggatttt cctttttatt tccgaatcgg agtgaaaagc
     1561 cgtatggcac tttctgaacg ttccaaattt gcgaagaaag ttgcttcgaa attagaagag
     1621 ctgacagcac ataaattaag aaattctacg tctcattatg aatttgagat ccgtatgatc
     1681 gaaggtaaat ctggggatta ttatttatta gtgaaattaa atacgatcgt tgatcgtcgt
     1741 tttagttacc gcgaggagtt cattccaaca agtatcaaac cagtcaatgc tgctttactt
     1801 gtggaacttg ccaaagatta catgatcccg gatgcgcaga ttcttgatcc tttctgtggg
     1861 gttggtacga tgctgatcga gcgtcagaag gttgtaaaag ggaatacttc ttatggtatc
     1921 gatcattctc cagaagccat tgagaaagcg atctataaca caaatcttgc agaccagatc
     1981 gttcattata tcaataagga ttgttttaca tttacccatg actatccttt tgatgaaatc
     2041 tttacggaga tgccttatgc gactggccag aagacagaag cggagattcg tgaggtttat
     2101 gagaagttct ttccatttgc caaaagagtt cttggacctg aaggaacaat cattatgtat
     2161 acaagaaacc gtgaatatgt aaaacagttc gctgtaaaat ccaattttag aattctgaaa
     2221 gaaatcaaga tcacacagcg gccggaaagt tatcttatga tcttaaaata ggttttttta
     2281 ggtaatttat aatacaagaa ccattctgta acatcattta tacggaatgg ttcttcttat
     2341 tttagatcgt cttatattta tttatgaaat ttttgattct tcgttttccc tcttccatat
     2401 cttttgaaaa tgctagtgcg tgtcctgctc ctgaaacaat atagagttct ttctttgctg
     2461 cacaggcttc ataatttttg atcgtcatcc acatcggaac gtaatcgtct gcatctccat
     2521 ggataaacaa cactggaatc ttgtttcgtt tcatgatttc tgggatgctg acttctttca
     2581 atccaaatcc tgctactact tcactgatca aatctaccat gtacattaat ggaaatggtg
     2641 gaagatgaaa tctttctttg gctacgtgtt tgatgatatc ccatggagaa gtgaatccgc
     2701 agtcagcaat aattcctttt acattgtctg gaaggtccag acctgctgcc attaaggttg
     2761 tagcacaccc catagaaatt ccacatagaa aaatatcttt gttcttggct gccagatgcc
     2821 gattggtata caaaatccat cgtttgagat catggcgttc tttcactcca taagtaatat
     2881 agcgtccttc gctttttccg tggcttctct gccatggaag aagaatgctg tatccaagat
     2941 tccatagaaa tcggacttcc ggtacgaatt cgatatcgtt tgttgaatgg taaccgtgca
     3001 tgcagataat agttcctttg gcattttctc ttgggatgta ggctgcccgt aatgttagcc
     3061 cgtcttttga tctgatttct tttaattctt gttcttggtg ttcttttagc cattcttgtt
     3121 cttttaaaac gtaatcaaga tatggtttcc atttttcttt tttacggaca tctggctgtt
     3181 tttttcttcg acagatcgca taatcgaaga cagtaaatgc agatgcactg acaatagttc
     3241 ctgtgatcgc tgttgcggtt aatagttttt gtttgttgtt catatttgga tcctcatttt
     3301 actgttcact gttttattct gtgatatcta aggttgtttc ccctgtgatt ttgattccat
     3361 gttcttttaa taatctagct gtgattccgt ccccattgat cagcgtgcct gtaaatgttc
     3421 catcatagac tttgccacag ccacaagatg gacttttttc ttttaggatc gctgtgtgac
     3481 agttatataa ctttgcaatt tttagtgtct cttcggcacc tttttggtat tgttttgtaa
     3541 catctttgcc atcttttgcg ataactttgt tttgatgtat ttctgccgga attcttggtg
     3601 ttggcagtcc tcccatgatc tctggacaga ctggaattag ttcatggtct tttagtaatt
     3661 ctatgatttt atcatttttg ttgttttttc cgttatattt gcaatcgatt cctaacaggc
     3721 aggcactgac taatattttc attaattttc ctttcttcta caaagctaat tcaaatccat
     3781 aacagctttt ttcaaatcca tagtgttcat aaaatccgtg agcttgtact cttggcagtc
     3841 cggagtctaa agtgattgct ttacatcctc gttcttttgc caatttcttt gcatgatcta
     3901 ataattttac accatatcct ttacctcggt cttcttcgtt tgtgatcaaa cttgatacat
     3961 agcaggtaag ccctgacatc cagaaggtct gaaaataatc tccatggcag aatccatgaa
     4021 aagttccatc gtcttctatt gcaaaaaatg caaaactgtt ttcgtcttgt agtacttttt
     4081 cgtagacttc tttggttgtt tcatagtcgt aggtgttgta gtcccagagt tttttgatgt
     4141 attcataggc tgtgtcgaaa tctttgattg ttgcattttt gatcttcatg ttggtgtcct
     4201 cctttgatgt ctgtttttta ttattgtagc atgtaaaatt tttctggtaa ataaaaagac
     4261 aggaagctga ccttctttgg tctgtttcct gtcttttttt aatcttataa tctctaatta
     4321 atctctaacc ttaatatgca cttcacgaag ctgctgttct gtaatctcgt ttggtgctcc
     4381 gcacatcaga tcctgtgctg ttccgctcat tgggaaagcg atgatctctc ggatattttc
     4441 ttcgttgcga agtaacatga tcatacggtc tactcctggt gccattcctg catgtggtgg
     4501 tgcaccgaac tggaatgcat tgtataatgc tccgaatttg tcttttaatg tttctttgtc
     4561 atatcctgcg atgtcgaatg ctttttccat gatttccatg tcatggttac gaacggctcc
     4621 tgatgataat tctactccgt tacatacgat atcatactgg tatgctaaga tatctaatgg
     4681 atctttttca tttaatgctt ctaatccacc ctgtggcata gagaatgggt tatgtgtaaa
     4741 tcctaattta cccgtttctt catcaatttc atacattggg aaatcattga tgtagcagaa
     4801 acggtatgcg tctttttcta acaggtctaa tctgcttcct aattcgttac ggatctgtcc
     4861 tgcatagatg caagcagctt tttctggtgc tgcgatgaag aagattgtat ctccttcctg
     4921 taatccagcc atttcagcta attccatctt catatcatct ggaataaatt tatcaatcgg
     4981 tcctttgtat ttcagttctt ctttgacttc taagtatcca agtccaccca taccgattga
     5041 ctgtgcgaat tttaacagtt tttcatgctg tcctttggaa agttttgcat gaacagtctg
     5101 accatggaat ggtttgaatg tacatctctg gaagaaatct gtcacgtcga tgattcttaa
     5161 tgggttacga aggtctggtt tatcactacc aaactgcagc attgcttctt tgtagctgaa
     5221 gattgggtat ggtgcttctg tgatcacact tccttctggt gcaaatttct taaatgtctc
     5281 tgttaatact tcttctccga tcttgaagac atcttcctgt gttgcaaatg ccatctcgaa
     5341 gtctaactga tagaattctc ctggagaacg gtctgcacga gcatcttcat ctctgaagca
     5401 tggtgcgatc tgatagtatt tgtcgattcc agataccatc agtaactgtt tatactgctg
     5461 tggtgcctgt ggtaatgcgt aaaatttacc tttgtatttt cttgaaggta ctacatagtc
     5521 acgagctcct tctggtgatg atgcacaaag gatcggtgtc tgaacttcta cgaatcccat
     5581 gtcttccatc ttttctctta agaaacggat gactttggaa cggaataaaa tgttatcttt
     5641 tacctttctg tttctcatat caaggtaacg atattttaaa cgaacatctt cacggatttc
     5701 tttacttgtt gcgatctcga atggaagatc tctgtatact tttcctaaga tatcgatctt
     5761 gtgtgcttct aattcaatcg ttcctgtagg aatcttaggg ttaaatgttt cttcgtctct
     5821 gtgctggatc acaccttcta cagataaaca ttcttctttt cttacatggt ctaataaact
     5881 tgtatctctt aatacgacct gcatcactcc atacatatcg cgtaagtcga taaatgatac
     5941 tccaccgtgg tcacggatat tctctaccca tccggcgatc tttaattgtg ctccgatgtc
     6001 accttcagaa atctggtcca tggttctggt acggtacatg tctgacattt tcttttctcc
     6061 tttttcttgt attctctatc ttgcgttcag gcggttttct tagttccctg tcaccttaaa
     6121 atcaggtaca aaaaaagccc gtcctgaaat tgttacttca ggacgaactg tatatctcta
     6181 gtccgcggta ccacctgatt tactgtcttg cagtctctct tgtgtgccgg ttaacgccca
     6241 gcttacgtcg cacctactga cagggtttga atcttgttct attcatgttc tgcgttcaga
     6301 ttgatgctcg ggagtgttct tcgcataaat tcactttgca ggattctcac tatcgcctac
     6361 tcactgggaa cgataattta tggtactttt ctccgtcttt gcctattgag tgattatatt
     6421 atattgagtt ttgttttgtc aatagttttt caatatgtat atttagattt tcggttttat
     6481 ttttaatcaa ttaggtcctg atcactaaat aatgcgttga ctgcctgttc gtaattcttt
     6541 agcgtctgaa ctttacggat cttctttgcg tatttttcgt ggtttgtaaa catgaccgct
     6601 gcattgttcc agaattcttt catcttatag aggacatttt tgtcgccgct taggtaaccc
     6661 tgatatgctt cataaagtgt atcatgcatt ttcaggagtt ctctttttgt cagtttctgt
     6721 ccatatttga tttctcgtac aagggctgga tttcttaaca gacctcttcc gatcatcact
     6781 gcatctacat ctggaaacct tgttgtgata ttgtcgtagt cttcttttgt tacgatatct
     6841 ccattgtagc agagtggatt ctcgctgtgg tttactgcat attcaaagac atccaggtgt
     6901 ggttttcctt tatagaattc ctgctgtagt cttggatgga tcgttagttc atagactgga
     6961 tggcggttgt agatgcttaa gatttcttca aattcttctg tgtctcttaa tccgattctt
     7021 gttttaatcg agatcttcat atctaatgct tcgaagatct ctgtaaggaa atcatctaat
     7081 acttctggtt ctcgcaggaa tcctgagcct ttttgtttgg ctacaacggt tcttgacggg
     7141 cagccaagat ttaaattgac ttcatcgtat cccagttctt ttaattcttt tgctgttctg
     7201 ataaaatctt cagagctttt cgttaaaatc tgtggtacga tatgtaatcc ttcattatgt
     7261 tccggcaata catcctgaaa ttctcttgtt ttgaatgttc tttttccatt tggcatgata
     7321 aatggtgtgt agtatttatc gatttctcca aatagttgtt cgtaaatgtt tcggtataag
     7381 taaatcgtga tactttccat tggggcataa taaaatttca taataacgat cagtttcctc
     7441 catttaaatt atacttcttg acactcatac cattatatat gatttacgaa ccgagggcaa
     7501 aatatattac aaatactgaa atcgccaatc cagactttat tttttcaaca attgatgtta
     7561 tattagtaaa caacgagtga aaatttgaga aacattgata caatttatca gcgaggtatg
     7621 aataatgaaa gaaatgaaac cgatcaaaga aggtaaagtt cgtgagattt atgacaatgg
     7681 tgacagcctg atcatggttg caactgaccg tatcagctgc tttgatgtca tcttaaagaa
     7741 tgatgtcaca aagaaaggaa ctgttttaac acagatgtcc aaattctggt ttgaccttac
     7801 aaaagacatc gttcctaacc atatgatctc tgttgatgtc aaagatatgc ctgaattctt
     7861 ccaacaggaa aaatatgatg gaaacagcat gatgtgtaag aaacttacta tgcttccaat
     7921 cgaatgtatc gttcgtggat atatcacagg aagcggttgg gcaagctatc aggagaatgg
     7981 tacagtctgc ggaatcaaac ttcctgaagg attaaaagaa tctgataaac ttcctgaacc
     8041 aatctacaca ccatctacaa aagcagaaat cggtgatcat gatgagaaca tttcttttga
     8101 aagaagtatt gaagttcttg agaaagaatt cccaggtaaa ggacaggaat acgctgaaca
     8161 gttacgtgat aaaacgatcg ctctttataa gaaatgtgct gattatgcat tagaacacgg
     8221 aatcatcatc gcagatacga aatttgaatt tggtcttgat gaaaacggta acatcgtcat
     8281 cggagatgaa atgttaacac cagacagctc tcgtttctgg ccagcagacg aatacgaacc
     8341 aggtcatgga cagccatcct tcgacaaaca gttcgcccgc gactggttaa agagcaacga
     8401 acatgactgg aaactgcctc aggacattgt tgataagaca atcgataaat atttccaggc
     8461 ttatgagatg cttactggga aagatttgta agtagtattt tgttgtatgg gatataaata
     8521 aaagaaaaag aaagatcatg aataagcaaa gtggcactcc gcatttttga aagcgtttt
//
LOCUS       DAOMUE010000052         8538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2120_length_8538_cov_3.593776, whole genome shotgun sequence.
ACCESSION   DAOMUE010000052 DAOMUE010000000
VERSION     DAOMUE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8538
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2120_length_8538_cov_3.593776"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLC71_03850"
     CDS             complement(<1..234)
                     /locus_tag="PLC71_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRM01810.1"
                     /translation="MKQLKKYKHFLMRLLNLVLIVGVCFAYHNIATIRAEKEAKIAAE
                     NSGSGLWKDGTYEGSGQGFGGQIVVSVTIKNGSI"
     gene            complement(256..1854)
                     /locus_tag="PLC71_03855"
     CDS             complement(256..1854)
                     /locus_tag="PLC71_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRM01811.1"
                     /translation="MKIIDQLKKVNLIQFVPAIFVISLVGNSVFGYTAPKSEKSKNNS
                     ENQVEAATTQAKKKTKKANAGDIDLSKIKDGTYEGQANGYRGLVKVSVTVKDHKITAI
                     KVLSNSDDAAFFNRASAGVIKNILAKQSLKVDVVSGATYSSNGIIKAVKNALTGEEDK
                     SSAKAGGSSAKSAGSVGTADESGTYKDGTFVGTAAGYHGTVKVSVTIKKNKIKSIKIL
                     ENHDDAAYFNRAKGILLPLMVKKQSTNVDAVSGATFSSNGIIKAVRNALSKAAVNKSS
                     SDSSSTSTTKDTTKKDTTEKVSGTYKDGVYEGTGTGFRGKITVSVRIKKSKIVEIKLV
                     KNEKDDAAYFERAWSEVPFSIIAMQSADTDKVDAVSGATYSSKGIMAAVRDALKKALA
                     KKDTETTVETNNTNNSTNDNNTNTDNSSEDNTTTVPTTPTDQTYTGSAVCEPDEDGDF
                     DAYDLTLEVVVSSDGKVKGIQNIKWSDKSMQSWYKDAEKKIVPQLIANGLDTSKNYDV
                     VTGATCSSNALINAYKNAISKINQ"
     gene            complement(1892..2950)
                     /locus_tag="PLC71_03860"
     CDS             complement(1892..2950)
                     /locus_tag="PLC71_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRM01812.1"
                     /translation="MKLKKLICIVMISVLGVLSFAGCAKKQDAKKTETSSEASTQEAS
                     AQVFAMDTYMNLKAYGKNAQKAVDDAKSEIERLDQLWSAVDKNSEIYQLNQKKKMKVS
                     DETLELIEFAKKKSKESNDAFDISIYPIVELWGFPTQKYRVPSDSEIQKFLKNVNSQK
                     IKINKKTNVVTLEKNMKIDLGGIAKGYTSQRIAKIYKKDGVKSGVISLGGNVQAIGTK
                     TDGSRWKVGVQSPDDTESMIGAYEAADEAVITSGAYERYFEKDGKTYHHIIDPATGKP
                     SEKDLKSVTIISKNGTLSDTLSTTLFVMGKDKAISYWKEHSKEFNMILVDENNKVYIS
                     QGIKDHFSSDSDFTVIKK"
     gene            complement(3145..4314)
                     /locus_tag="PLC71_03865"
     CDS             complement(3145..4314)
                     /locus_tag="PLC71_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01813.1"
                     /translation="MKKMKSRSFRILMAAVISAAMLCPQVKISAADGLVSKAVTSYFP
                     SKTVMKKTARKTEPEEWNQKEIKAYKFGTLEDALYYALTGGIKYYDQNYFKNPDLIKL
                     TPVESGALGLAVVGDNKQEAILYDSNKKMIKKLPLSYVKAQVNAGETYYIEFPKNCKE
                     GLITAYVLQNECGGLAKNDLNMQKGEEKETYHTFKMTKRGFAGFVVASMVEDGGNTSY
                     KVQKNEKGKWITIGRTKSFKPTNEDETQIAVGYGLSKGNYRLVLKAPKEQLNTMLYTT
                     KNYAKKKVAYKKSKAKNLNATEMYTMNEKAARWYKVSVKSSKKQSKLKILTVADQGGF
                     KFTIYERGKKKPVKTVKTSAKHLEKTVKLPKKKGMYYVKVSKRTKKTNGYYEIKK"
     gene            complement(4337..5497)
                     /locus_tag="PLC71_03870"
     CDS             complement(4337..5497)
                     /locus_tag="PLC71_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01814.1"
                     /translation="MTRMGIKKMGAVLIAAGLSIAMCSPSVSANVRTNINTDKITQKK
                     TARYVEPEEWNKEELTAYQFDSEVDMMKYLVSGGMHLYNKNYKGSDRWVKIKVADPGL
                     FMVGVVSNDETKIPVYDAAKKRIIVNNLNDGGDKEYVGIVKTGDEFYVKLPEKIDKVI
                     ILTGVIKTEFTSMANQKSYYELGKGTTTYHPFSVAKRSKVQFDITSIGKKHEKVGVYI
                     EKNVNGTWKKVGYSTTVKPDKYDSTVLYGLEAGKYRLALKTPKDQIINVEYTRDKSKK
                     KAAYKKSKAIHLKSDIDSIYTSTEKAARWYKVSVSSTKKRKAVTLSKDSVGGGFKFCV
                     YQKGKRLKTVKVTKNDKVKTVKLPKKKGAYYVKVTKLTSKTTGAYYLGTYTY"
     gene            complement(5527..6672)
                     /locus_tag="PLC71_03875"
     CDS             complement(5527..6672)
                     /locus_tag="PLC71_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01815.1"
                     /translation="MRKILKKTATLMMTAVIGVSMSCPKMIVYAGQRMQMQERYTTPK
                     EWNYTEITAYQFGSTDKFEDYLQTGGTHLYNDKFQEKDHMVKITVADSGYFTIATQTD
                     GNRSQKINLYDSTKEKVLAQTTSDGDLEYGRLAKAGEVFYLQMPEKIDKIFVTTGVIK
                     DGFGSMKASDTYYESGTGSTTYHPFSIKKRSAVEFNISAIDRNSGITYAHIEKKENGQ
                     WKRIDDTVKIKPASYDDDFVHGLTKGEYRLAIKAPTTQLNAVSYTSSSKSKKVAYKKS
                     KAKKIKLDGQTSNIYTTGEKTSRWYKISITSTKKKRILNLGKNTVSGGYKFTIYKKGK
                     KKAIKTIKVTGNANAKIAKMPKKKGTYYIRISKLTKKTNGTYEIGYY"
     gene            complement(6711..7250)
                     /locus_tag="PLC71_03880"
     CDS             complement(6711..7250)
                     /locus_tag="PLC71_03880"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRM01816.1"
                     /translation="MTKKEARQQVKEWMKKLDKNYIEKADQSIFEQVKELKEFQEAKT
                     IFCYASMKNEVDTWQLIALALKQGKNVGVPLCIGKGIMEVRQIHALDDFEEGAYGIME
                     PSRRCPILSKEKIDMAVIPCVSADREGRRLGHGAGFYDRFLDGVTFPKILVCYKELMM
                     DQVPTEEHDILMDQVICDE"
     gene            complement(7346..8251)
                     /gene="pfkB"
                     /locus_tag="PLC71_03885"
     CDS             complement(7346..8251)
                     /gene="pfkB"
                     /locus_tag="PLC71_03885"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HRM01817.1"
                     /translation="MIYTVTFNPAIDYVIKVDNLTLGQVNRTTREYIFCGGKGINVSH
                     LLANLGVDSVGLGFIAGFTGDVIESGAKELGFKPDFIRVREGMTRINVKVKSNEESEI
                     NGLGPVIHDDELEQMFKKLDELEDGDILVLAGSIPATLPSDVYERIMGRLQGKDIRIV
                     VDATKDLLLNVLKYHPFLIKPNNHELGEMFDVVLKTNDEIVEYAKKLQEKGARNVLVS
                     MAGDGAILITEEGEVHVTPCPKGEVKNSVGAGDSMVAGFITGYLRSNGDYNEALKMGT
                     AAGSATAFSEDVATGEKIMELYKTL"
     gene            complement(8248..>8538)
                     /locus_tag="PLC71_03890"
     CDS             complement(8248..>8538)
                     /locus_tag="PLC71_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HRM01818.1"
                     /translation="GEEALEQLDKYNFTKGFFGTNGIDLKRGFTTPDQKEAAVKKKAM
                     EQCQKTYILSDASKFNVISTIKFADLKKAEIITDHLENKEFKRITNIEEVFS"
BASE COUNT         2085 a         1868 c         1158 g         3427 t
ORIGIN      
        1 gatagaacca ttttttatgg ttacactcac aacaatctga ccaccgaatc cctgaccgct
       61 gccttcatag gttccatctt tccataaacc cgaaccactg ttctcagctg cgatcttcgc
      121 ttctttttct gcccggatcg ttgcaatatt atgatatgca aaacatactc caacgatcag
      181 cacaaggttt agaagtctca ttaagaaatg cttgtatttt tttaattgtt tcatatctct
      241 tttttgttcc tctacctatt ggtttatctt tgaaattgcg ttcttgtatg cattgatcaa
      301 tgcattgctt gaacatgttg ctcctgttac cacgtcataa ttttttgaag tatccaaacc
      361 atttgcgatc aattgaggaa ctatcttttt ttccgcatcc ttataccatg attgcatgct
      421 tttatctgac cattttatat tttggattcc ttttactttt ccatcagatg aaactacaac
      481 ttctaaagta agatcatatg cgtcaaaatc tccatcttca tctggctcac atactgcact
      541 tcctgtatac gtttgatctg tcggtgttgt tggtacagtt gttgtattat cctcagatga
      601 attatcagta ttcgtattat tatcatttgt actattgttc gtattatttg tttctactgt
      661 agtttccgta tcttttttcg ccaatgcttt ctttaacgca tctctgactg ctgccatgat
      721 accttttgaa ctatatgtag caccactaac tgcatcaact ttatctgtat ctgcactctg
      781 cattgcaata atactaaatg gaacttcgga ccatgcacgt tcaaaatatg ccgcatcatc
      841 tttttcattt ttaacaagtt taatctctac gatcttgctc ttcttaatgc ggacactgac
      901 agtgatcttt cctctgaatc ctgttccagt tccttcgtaa acaccatctt tgtatgtacc
      961 tgatactttc tctgttgtat ctttcttcgt tgtatccttt gtcgtagatg tagaactgct
     1021 gtcactagac gatttattaa ccgctgcttt gcttaatgca tttcgtaccg ctttgataat
     1081 tccattggaa ctaaatgtag ccccgctgac tgcatctaca tttgtactct gtttcttaac
     1141 catcaatgga agtaaaatac cttttgcacg gttaaaatac gctgcatcat catgattttc
     1201 caaaatttta attgatttga tcttattctt tttgatcgtc acagaaactt ttacagtacc
     1261 atgatatcca gcggctgtac caacaaatgt accatctttg tatgttcctg attcatccgc
     1321 tgtaccaaca cttcctgcag atttcgcaga agatccgcct gcttttgcgg atgatttatc
     1381 ttcttcccct gttaacgcat tttttactgc cttaatgatt ccatttgaac tgtaagttgc
     1441 tccactgaca acatcaactt ttaatgactg ctttgccaga atatttttga tcacacctgc
     1501 tgatgcacga ttgaaaaatg cggcatcatc ggaattactt aatactttga tcgctgtgat
     1561 cttatgatct tttacagtaa ctgaaacctt taccaaacca cgatatccgt ttgcctgtcc
     1621 ttcataagtt ccatccttga tcttgcttag atcaatatcc ccagcatttg ctttctttgt
     1681 tttctttttc gcttgtgtcg ttgcagcttc tacctgattt tcagaattat tttttgattt
     1741 ttctgacttt ggtgccgtat atccaaaaac actgtttcca accagactga tcacaaaaat
     1801 cgcaggcaca aactgaatca gattgacttt ctttaactga tcaatgatct tcatttttgt
     1861 ttttccttct ctctttctat ctgttaacgt tttatttttt tattactgta aaatcactat
     1921 ctgaactaaa atgatctttg attccctgtg aaatatatac cttattattt tcatcaacaa
     1981 ggatcatatt gaattctttg ctgtgttcct tccagtaaga aatagcttta tccttcccca
     2041 taacaaacaa tgttgttgac aatgtatcag ataatgtacc atttttgctg atgatcgtta
     2101 ctgatttcag atctttttcg gatggttttc cagttgctgg atcaatgata tgatgatatg
     2161 ttttcccatc tttctcaaag tatctttcat atgctccaga tgtaatgact gcttcatcag
     2221 cagcttcata tgcaccgatc atactttctg tatcatctgg tgactgcact ccgactttcc
     2281 atcggcttcc atctgttttt gttccgattg cctgtacgtt accaccaaga ctgatcacac
     2341 cgcttttcac accatccttt ttatagattt ttgcaattct ttgtgatgtg tatcctttag
     2401 caattcctcc aagatcaatc ttcatattct tctctaatgt cacaacattt gttttcttat
     2461 taattttgat tttctgacta tttacgtttt tcagaaattt ctgaatttca ctgtccgatg
     2521 gcacacgata tttctgtgtc ggaaatcccc ataattccac gatcggatag atagaaatat
     2581 caaaagcatc gtttgattct tttgattttt tctttgcaaa ttcgatcaat tctaatgtct
     2641 catcagatac tttcattttt ttcttctgat tcagctgata gatctcactg tttttatcta
     2701 ccgctgacca tagctgatct aatcgctcaa tctcactctt agcatcatcc actgcttttt
     2761 gcgcattctt tccatatgct ttcagattca tataggtatc catggcaaaa acttgcgcac
     2821 ttgcttcctg tgtacttgct tcactgcttg tctctgtctt ctttgcgtcc tgtttcttgg
     2881 cacatccagc aaacgataat acccctaaaa cactgatcat cactatacat attaactttt
     2941 ttagtttcat ttatttcatc cttctaggtt agcatttact aacctatagt ttataatttt
     3001 tctgcttttg tttcaagaat agtaattgag gttttttttc attaataaaa tactttgcca
     3061 ttctatagta attttatata aaaaaggttc aaaaaaggaa ctgtcttttc tgacaattcc
     3121 ttttttgacg ttctattcta accgttattt cttaatctca tagtatccat ttgtcttctt
     3181 agtacgcttg cttactttga cataatacat tccttttttc tttggaagct ttactgtctt
     3241 ttctaagtgt tttgcagatg tttttactgt cttaactggt tttttcttac ctctctcata
     3301 gatcgtaaac ttaaatccac cttgatctgc aactgttaaa atcttcaatt tgctttgctt
     3361 tttgctagat tttacagata ctttatacca acgggctgct ttttcattca ttgtatacat
     3421 ctctgttgca tttagattct ttgctttgga tttcttatag gcaactttct ttttggcata
     3481 attttttgtc gtatacaaca ttgtattgag ctgctctttt ggagccttca atactagacg
     3541 gtagtttcct ttactaagtc cataacctac tgcaatctgt gtttcgtctt cgtttgttgg
     3601 tttaaatgac tttgttcttc cgattgtgat ccattttcct ttttcatttt tctgaacttt
     3661 atagcttgta tttccaccat cttctaccat ggatgcaaca acaaaacctg caaatcctct
     3721 ttttgtcatt ttaaatgtgt gataagtttc tttttcttca cctttttgca tattgaggtc
     3781 gttttttgct aacccaccac attcattttg taatacgtaa gctgtgatca atccttcttt
     3841 acagttcttt ggaaactcta tataataagt ttctcctgca tttacctgcg cctttacata
     3901 ggacagtggc aatttcttga tcattttttt attggaatca tatagaattg cttcctgttt
     3961 attatcccca acaactgcca gtcctaaagc tcctgattct actggagtga gcttgatcag
     4021 atctggattt ttaaaataat tctgatcata atatttgatc ccgccagtca gcgcataata
     4081 taaggcatct tcaagagttc caaatttgta tgctttaatt tctttttgat tccattcttc
     4141 cggctctgtt tttcttgctg ttttcttcat tactgtttta ctcggaaaat atgatgtaac
     4201 tgcttttgat actaagccat ccgctgctga tattttaacc tgcggacaca gcatcgctgc
     4261 tgatatgact gctgccataa gaatacgaaa tgacctactt ttcatttttt tcatcctgtt
     4321 tccctccttg tgattcttaa tatgtatatg ttcccaaata atatgcccct gttgttttgc
     4381 ttgtaagttt cgtaactttc acataatatg ctcctttttt ctttggaagt tttactgtct
     4441 taactttatc attctttgtg acttttactg tttttaatcg ttttcccttt tgataaacac
     4501 agaatttaaa acctccgcct acagaatctt tggacaacgt aactgctttt ctttttttgg
     4561 tagatgaaac agaaacctta taccaacggg ctgctttttc tgtggaagta tatatactgt
     4621 caatatctga ttttagatgg atcgctttag atttcttata agctgccttt ttctttgatt
     4681 tatcccttgt atattctaca ttaatgatct gatctttcgg tgtttttaac gccagacgat
     4741 attttcctgc ctctaatcca tagagcactg tactgtcata tttatctggt tttaccgttg
     4801 tgctgtatcc gacttttttc catgtcccat ttacattttt ctcaatgtat actccaactt
     4861 tttcatgttt ctttccgatg gatgtaatat caaattgtac ttttgagcgt ttcgctacgg
     4921 aaaatggatg ataggttgtt gtcccttttc caagttcata ataacttttc tgattcgcca
     4981 tggaagtaaa ttcggttttg atcacaccag ttaaaatgat cactttgtct atcttctctg
     5041 gcagttttac ataaaattca tctcctgttt tcacgatacc tacatattct ttgtccccgc
     5101 catcatttaa attatttact ataattcttt tctttgccgc atcatatact ggtatttttg
     5161 tctcatcgtt acttacgaca ccaaccataa acagccctgg atctgcaact ttgatcttta
     5221 cccagcggtc actgccttta taattcttat tatataaatg cattccaccg ctcaccagat
     5281 atttcatcat atctacttct gaatcaaact gatatgcagt caattcctct ttgttccatt
     5341 cttctggttc aacatatctg gctgtctttt tctgtgttat cttgtctgta ttaatatttg
     5401 tccttacatt tgcagatact gatgggctac acattgcaat gcttaaacct gctgctatca
     5461 gaactgctcc cattttcttg attcccattc ttgtcataga aattcctcct tatattttga
     5521 tttggattaa taatacccta tttcatacgt tccatttgtt ttctttgtaa gctttgagat
     5581 tctgatataa tatgttcctt ttttctttgg cattttcgct atcttcgcat ttgcatttcc
     5641 tgttacctta attgttttaa ttgctttttt ctttcccttt ttataaattg taaacttgta
     5701 tcctccagaa accgtgttct ttccaagatt cagaattctt ttcttctttg tagatgtaat
     5761 acttatttta taccatcttg aagttttctc tcctgttgta tatatattcg atgtttgtcc
     5821 atccagtttg atcttctttg cctttgattt cttatatgct acctttttac ttttagaact
     5881 gcttgtatat gagactgcat ttaactgtgt tgtcggtgct ttgatcgcca ggcgatattc
     5941 acctttagtc aatccatgaa caaaatcatc atcataagat gctggcttga tctttaccgt
     6001 atcatcgatt cttttccatt gtccgttttc tttcttttcg atgtgtgcat aagtgatccc
     6061 actgtttcga tcgatcgctg aaatgttaaa ttccaccgca gatctcttct taattgaaaa
     6121 tggatgatat gtggtacttc cagttcctga ttcataataa gtatctgatg ctttcataga
     6181 tccaaatcca tctttgatca cacctgttgt cacaaatatt ttatcgattt tttctggcat
     6241 ttgaagatag aagacctcac ctgcttttgc aagccttcca tattccagat ctccatcaga
     6301 tgtcgtttga gctaaaactt tctccttcgt tgaatcatag agatttatct tctgcgatct
     6361 atttccatct gtttgtgttg caattgtaaa ataccctgaa tctgcaaccg taattttgac
     6421 catatgatcc ttttcctgaa atttatcatt gtatagatgt gtaccacctg tctgaagata
     6481 atcttcaaat ttatctgtag atccaaactg atatgctgtg atttctgtat aattccattc
     6541 ttttggagtc gtatatctct cttgcatttg cattctctgt cccgcataaa ctatcatctt
     6601 gggacaactc atagatactc cgatcactgc tgtcatcatt aatgtcgctg ttttctttaa
     6661 aatctttctc atgtctatca tctctcctgc ttgatcattc tattacttca ttattcgtca
     6721 caaataacct gatccatcaa aatatcatgc tcttctgtcg gtacctgatc catcattaac
     6781 tctttataac ataccagaat ctttggaaat gttactccat ctaaaaaacg gtcatagaat
     6841 cctgctccat gtccaagtct acggccttct ctatctgcac ttacacaagg tattaccgcc
     6901 atatcaatct tctctttcga taagattgga caccttctgg atggttccat gattccatat
     6961 gccccttcct caaaatcatc taaagcatga atctgtctca cttccatgat tcctttccca
     7021 atacacaatg gaactccgac attcttcccc tgtttcagtg ccaatgctat cagctgccat
     7081 gtatcaactt catttttcat acttgcataa caaaaaatag tttttgcctc ttgaaattct
     7141 tttaactctt ttacctgttc aaaaatcgac tgatctgctt tttctatata atttttgtct
     7201 aattttttca tccattcttt tacttgttgt cttgcttctt ttttcgtcat gatgtcctcc
     7261 agtttttata aaattacgca caaaaaatga acctaatttc ttaggttcat tttatcataa
     7321 ttttctttca ttgtatgcta tttcattata atgttttata taattccata atcttctcgc
     7381 cagttgcaac atcttctgaa aatgcagtcg cacttcctgc tgctgtcccc atcttcagtg
     7441 cttcattata atctccatta cttctcaaat atcctgtgat aaatcctgct accatggaat
     7501 ctccagctcc tacagagttc ttaacttctc cttttggaca aggtgttaca tgaacttctc
     7561 cttcttctgt gatcaggatt gctccatctc ctgccatgga aactaacaca tttcttgcgc
     7621 ctttttcctg aagtttcttt gcatactcaa caatctcatc atttgtcttt agcacaacat
     7681 caaacatttc tcctaattca tgattgtttg gtttgatcag gaatggatga tattttaata
     7741 cgtttaacaa caagtctttt gttgcatcta caacgattcg gatgtctttt ccctgcagac
     7801 gtcccatgat tctttcatat acatctgatg gaagtgttgc tggaatactt cctgcaagaa
     7861 ctaaaatatc tccatcttct aattcatcta atttcttaaa catctgctct aattcatcat
     7921 catgaataac tggtcctaat ccattgatct cgctttcttc gttggatttt accttaacat
     7981 tgatacgtgt cattccttca cgtactctga taaaatctgg cttaaatcca agttctttcg
     8041 ctcctgattc aataacatct cctgtaaatc cagctataaa tccaagtcct acactatcta
     8101 ctccaagatt agcaagtaga tgagatacat taataccttt tcctccgcag aaaatgtatt
     8161 ctcttgttgt cctgtttacc tgtcctaatg tcaggttgtc tactttaatt acataatcaa
     8221 ttgctggatt aaatgttact gtataaatca tgaaaatacc tcctcaatgt ttgtgattct
     8281 tttgaactct ttgttttcta aatgatctgt aatgatctct gccttcttta aatctgcaaa
     8341 tttaatagtt gagatcacat tgaatttgct tgcatccgaa agaatatatg ttttctggca
     8401 ctgttccatc gctttcttct taactgctgc ctctttctga tctggcgttg taaatccccg
     8461 cttcagatca atcccatttg ttccgaaaaa gcccttagta aagttatatt tatcaagctg
     8521 ttctaatgct tcttctcc
//
LOCUS       DAOMUE010000053         8533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2123_length_8533_cov_2.400330, whole genome shotgun sequence.
ACCESSION   DAOMUE010000053 DAOMUE010000000
VERSION     DAOMUE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2123_length_8533_cov_2.400330"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PLC71_03895"
     CDS             complement(<1..821)
                     /locus_tag="PLC71_03895"
                     /inference="COORDINATES: protein motif:HMM:NF018406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HRM01819.1"
                     /translation="MDKSEKISWFEQFKIACLKPSQYKRLLNLAKGKVILFLAAITLI
                     TTILGYGMDVAGFTVSVGGWKNFILNRLPAFELKDGTLSVDQEMDFEIGGVHFVADTS
                     KDKVSTEDLSNKYQMELVFAKNEMVVKNTAVGNMMNTFSFKNMKKVEFNNQAMLSMVP
                     MIRIFIVIMFFTQWIANIISYLTVCIFITMLTYFNQRTRMDRKNREDVSFGKIFKLSI
                     YARVIFELVETIGVTAGSAIFTGMAWMFISYFGSYQLLLAGFMRPEDRQKPDEML"
     gene            complement(828..1382)
                     /locus_tag="PLC71_03900"
     CDS             complement(828..1382)
                     /locus_tag="PLC71_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRM01820.1"
                     /translation="MMGKVFVFLADGFEEIEGLTVVDMLRRAEIPTVTVSIGSSRNII
                     GAHRIEVEADIMFHEVLEAEGAMYVLPGGMPGTLHLKDHEGLGKLLQKAYKNEKYLAA
                     ICAAPTVFEKYGFLEGRKATSYPAMEEELKSADYQTDKVVVDGKIITSRGMGTAIDFA
                     AKLVEIIKGTKEKDELLKSIVYGE"
     gene            1575..1648
                     /locus_tag="PLC71_03905"
     tRNA            1575..1648
                     /locus_tag="PLC71_03905"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1609..1611,aa:Arg,seq:cct)
     gene            1711..2628
                     /locus_tag="PLC71_03910"
     CDS             1711..2628
                     /locus_tag="PLC71_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRM01821.1"
                     /translation="MKGLFIINPSSGKQNIDSMLREIMATLILDQICSHVDVFYTKGK
                     DDAKNRAAELKPGDYDFIVSVGGDGTLNEVTNGLILSESNIPMAIISAGTVNDFATYM
                     KLPQTADGFCQMIKDFQTKKVDVGRVNNEYFINVLAAGMLTDIAYKVPKDKKAVLGKM
                     AYYLEGVKEFPKQLSQNMTLTFNSKEYSGTEDIMLFLVANSKSVGGFADAAPLASVSD
                     GYLDVMIIKKMALLTEAPDLIFKLFQGEHPKHPSIEYFQTKELSVLPTEQTAGIAIDY
                     DGEILEEGLPVDISIVPEALNLIILRTTN"
     gene            complement(2642..3766)
                     /locus_tag="PLC71_03915"
     CDS             complement(2642..3766)
                     /locus_tag="PLC71_03915"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRM01822.1"
                     /translation="MKKIIKKITAMAVMSLFAATQLQTPQVNVQAADGVATAEKTTAK
                     QPKIYGRSGIVIDAKSGKILYAKKVDDRHYPASITKILTTLVAIENNKDLYDEVTFSS
                     YAVNSIEPGSTHIGIKAGEKIPLIDVLNAIMLESANEACNGAAEHTAGTTKKFVELMN
                     KKVKELGCTGSHFVTTNGLHDDDHYVTARDMAKISKAALENETFRYVACKPNYYIAPT
                     NKDKHGKELWNHHKMVKRTMFNYDGVEGGKTGYTTKAGGTLVTFAKRGDQELIVVDLC
                     AHGYELYEDTIKMLNYGFNNYKTIAPFKTMNTVLKDDTYGFLKTGNELSPYKIPLKHL
                     NDVTVMLEKNQSASKLTYKCKDNKYIISYDGKQIGSVKLH"
     gene            complement(3787..4569)
                     /locus_tag="PLC71_03920"
     CDS             complement(3787..4569)
                     /locus_tag="PLC71_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM01823.1"
                     /translation="MSKIIFFDIDGTLYDSRIGIPESTVNALNRLIANEHKIVMCTGR
                     SKGMIPEEYFHMGFDGVILGAGTYVEYEGKILHQELMTPEEVQTVIDWGKKQKIGIIL
                     EGEKYGYYDPENTDDYYIAMKNKTEADCKTTLYPLNEAVDVPKWTYHHMELSKKPEIE
                     EILGHKYKGTYHEPVNSVEFVPLGVNKAKGIEVILDHTGISREDSYAFGDSANDIDMI
                     KYVKYGVAMGNSVPELLEIAPYQTARVDEDGIEKGLKKFGLI"
     gene            complement(4588..5262)
                     /gene="ung"
                     /locus_tag="PLC71_03925"
     CDS             complement(4588..5262)
                     /gene="ung"
                     /locus_tag="PLC71_03925"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRM01824.1"
                     /translation="MPPITNDWADYLKEEYKKPYYRKLYQKVMDEYHTKLIFPPADDI
                     FNAFHFTPVKDVKVVILGQDPYHGDGQAHGLSFSVKPGVEAPPSLVNIYKELNEDLGC
                     YIPNNGYLKKWADQGVMMLNTVLTVQAHRANSHRGIGWEEFTDAAIRVLNQQDRPIVF
                     MLWGRPAQSKKALLDNPNHLILEAPHPSPLSAYRGFFGCHHFSQANEFLKEHGLAPID
                     WQIENI"
     gene            5368..6924
                     /locus_tag="PLC71_03930"
     CDS             5368..6924
                     /locus_tag="PLC71_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HRM01825.1"
                     /translation="MKSLLENPHGFSVSLVFDGLLVGIFAGCISVIYRLLLNNAEKLL
                     FTIIAFTKTRPFWIAVWFIVLIVMGLIVGWLMSWEGMASGSGIPQVQGELKGYLNQNW
                     HRVLCSKIIGGTLCILGGLSLGREGPSVQLGAMVAKGIAKITKKSQTKERYMMTCGAG
                     AGLAAAFNAPLAGVMFSLEELQKNFNSSMLVCIISGCVTSDFISKNVFGLSPVFDFHL
                     KAALPLVHYWMLILLGILLGLCGAFYNFIMLKGQDLFGAMKKIPAKYRIVFPFVVSGI
                     VCYTLPSILAGGHAMISLITGHTLLVSTMLLLLVAKFFFSAFCFGSGAPGGIFFPLLI
                     LGAYLGAIYGTLVIQASGIPSYYLVNFITISMAGFFTAIVRAPITGILLIAEMTGTFE
                     HFLSLAVVCIISYLVAHLLKSEPIYESLLGRILAKNGFKESEDADHKVLRGFAVGTGS
                     IAAKQLVKDIPWPEHCLIVTLNRGDEEIIARGSTEIHVGDTIIALIDDNYLGMVTESI
                     QLICGEIIPE"
     assembly_gap    7386..7395
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7396..8200)
                     /locus_tag="PLC71_03935"
     CDS             complement(<7396..8200)
                     /locus_tag="PLC71_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM01826.1"
                     /translation="MNVSSTFKLNNGNEIPVMALGSWDSRGDEAYQACLDALSSGYRH
                     IDTAAYYENEALVGAAVRDCEIPREEIFVTSKMWYEDMVEGKQEAAFEKTLKELGLEY
                     VDMYLLHWPINDVKGSWKVLEKYYEQGLIKNIGVSNFRPKHLKQLEVTANIAPAVDQI
                     EICPFLVQEETVNYCQEHDIVVEAWGPLGKGKQLSEPEIVAIAKKYNKTPAQIILRWH
                     LQRGIVSLPKSVHKERIIQNADIFDFELSKEDMQIMMSMDLRESTGRGYP"
     gene            8351..>8533
                     /locus_tag="PLC71_03940"
     CDS             8351..>8533
                     /locus_tag="PLC71_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM01827.1"
                     /translation="MDRKLKLPIGIDNFEKIRKENFYYVDKTKLIEQLLERWGEVNLF
                     TRPRRFGKTLNMSMLKY"
BASE COUNT         2364 a         1742 c         1453 g         2964 t
ORIGIN      
        1 tacaacattt cgtctggttt ctgacggtct tccggacgca taaatcctgc aagtaaaagt
       61 tgataacttc caaaataaga aataaacatc cacgccattc ctgtaaatat cgcagaacca
      121 gcagtaacac caatcgtttc caccagctca aatataactc tggcataaat agacagctta
      181 aagatctttc caaaggatac atcttctcga ttcttacggt ccattcttgt tctctgatta
      241 aaatatgtga gcattgtaat aaaaatacag actgttaaat aagaaataat atttgcgatc
      301 cattgtgtaa agaacatgat cacgataaaa atacggatca tcggcaccat agatagcatt
      361 gcctgattat tgaattcaac tttcttcata ttcttaaatg aaaaagtatt catcatgttt
      421 ccaacagctg tattctttac caccatctca ttcttagcaa ataccagttc catctgatac
      481 ttattgctta aatcttcagt tgatacttta tccttggatg tatctgccac aaaatgtaca
      541 ccgccgatct caaaatccat ctcctgatca acagataatg ttccatcttt taattcaaat
      601 gcaggcagtc tgtttaaaat aaaattcttc catccaccaa cactgactgt aaatcctgca
      661 acatccattc catatccgag aatcgttgtg atcaatgtga tcgctgcaag aaataaaata
      721 actttccctt ttgcaaggtt aagaagtctt ttgtactggc ttggttttaa gcaggcgatc
      781 ttaaactgct caaaccagct gattttctct gatttgtcca taaatgatta ttctccgtat
      841 acaatacttt tcagtaattc gtccttctct tttgttcctt tgatgatttc tactaatttg
      901 gctgcaaaat cgatcgctgt tcccattcca cgacttgtaa tgatttttcc atctactaca
      961 actttatctg tctggtaatc tgcggatttt aattcttctt ccatcgctgg atatgaagtt
     1021 gcctttcttc cttctagaaa tccatatttt tcaaatactg ttggtgctgc acagatggct
     1081 gcaagatact tctcgttctt atacgccttc tgaagtaatt ttccaagtcc ttcatgatct
     1141 tttagatgaa gcgttcctgg cattcctcct ggaagtacat acattgctcc ctccgcttct
     1201 aatacttcat gaaacatgat atctgcttca acttcaatac gatgtgcacc aatgatattt
     1261 ctggaacttc cgatagagac tgtaactgtt ggaatctccg cacgtcgcag catatcaact
     1321 acagttaaac cttcgatttc ttcaaatcca tctgctaaaa atacaaaaac ttttcccatc
     1381 ataattcttc tcctttattc tttctatatt ataacatcgt tcttcttttc ttcctcttct
     1441 ttattattac aataacttta aagaaaaaat ggtaaaaagt caacccgtct tagccttact
     1501 ggtatttctt ttctattttc aattgacatt ttcgttaaga atcggtaaaa tagatttcga
     1561 taaattatat gtatgtttcc gtagctcagc tggatagagc gaccgcctcc taagcggtag
     1621 gccgggtgtt cgaatcacct cgggaacact tcatgtgccg gagacttatg cctcgtttcc
     1681 ggcactttat gtttataagg aggaatctca atgaaaggtt tatttattat caacccatca
     1741 tccggtaaac aaaatattga ttctatgtta cgagagatca tggcaacatt gatccttgat
     1801 caaatttgtt cccatgttga tgttttttac acaaaaggca aggatgatgc gaagaatcgt
     1861 gctgccgaat taaaaccagg agattatgat tttattgtat ctgttggtgg tgacggtaca
     1921 ttaaatgaag ttacgaacgg tctgatctta agtgaaagta atattcctat ggcaatcatt
     1981 tctgctggaa cagtcaatga ttttgctaca tatatgaaac ttcctcagac tgcggatggt
     2041 ttctgtcaga tgatcaaaga cttccagaca aaaaaggttg atgttggacg tgtcaataac
     2101 gaatacttca tcaatgttct ggctgcaggt atgttaacag atatcgctta caaagttcca
     2161 aaagataaga aagccgttct tggtaagatg gcctattatc tggaaggtgt aaaagaattt
     2221 ccaaaacagc tttcacaaaa tatgacttta acatttaaca gtaaggagta ttctggaact
     2281 gaagatatca tgttgttctt ggtcgcaaac tcaaagagtg tcggaggttt tgcagacgcg
     2341 gcccctcttg catctgtttc tgacggatat ttagatgtta tgatcatcaa gaaaatggca
     2401 cttcttacag aagcaccaga tctaatcttt aaattattcc aaggagaaca tccaaagcat
     2461 ccttctatcg aatatttcca gacgaaggaa ttatctgttc ttccaacaga acaaactgcc
     2521 gggatcgcca tcgattatga tggtgaaata ttagaagaag gactcccagt cgatatctct
     2581 atcgtaccag aagcccttaa tctgatcatt ttacgtacaa ctaattaatc tgtactaata
     2641 attaatgaag tttcacagaa ccaatctgtt ttccatcata acttatgata tatttattat
     2701 ctttacattt atatgtaagt ttggaagcag attggttttt ttctaacatc actgtcacgt
     2761 catttaaatg ttttaatgga atcttatatg gggataattc atttcctgtt tttaagaaac
     2821 catatgtatc atctttcagt actgtattca ttgtcttaaa tggtgcgatt gtcttataat
     2881 tgttaaatcc ataatttaac atcttgatcg tatcttcata taactcatat ccatgtgcac
     2941 aaagatctac tacgatcagt tcttgatctc ctctcttggc aaatgttaca agagtccctc
     3001 ctgcttttgt tgtatatcct gttttaccac cttcaacacc atcatagtta aacattgttc
     3061 gtttcaccat cttatgatga ttccaaagct cttttccatg tttatctttg ttcgttggtg
     3121 caatataata attaggtttg catgcaacat aacggaatgt ttcattttca agtgctgctt
     3181 ttgaaatctt tgccatatct cttgctgtta cataatgatc atcatcatgc aatccgttgg
     3241 ttgttacaaa atgagaacct gtacatccaa gttcttttac ttttttattc atcagttcta
     3301 cgaatttctt tgtagttcct gctgtatgtt ctgctgctcc gttacatgcc tcattcgcag
     3361 attctaacat gatcgcattc agtacatcga tcaatggaat cttctctcct gccttaattc
     3421 cgatatgtgt acttcctggc tcaatgctgt taactgcata acttgaaaat gtaacttcat
     3481 catataaatc tttgttattc tcaatcgcaa ccaatgttgt taagatcttt gtgatacttg
     3541 ctggataatg acggtcatct accttctttg catacaaaat ctttcctgat ttcgcatcaa
     3601 taacgattcc tgatcttcca tagatctttg gctgttttgc cgttgttttt tctgctgttg
     3661 caacaccatc tgctgcctga acattcacct gtggtgtctg aagttgtgtc gctgcaaaca
     3721 atgacatgac cgccattgca gtaattttct tgatgatctt tttcatgatt ttctctcctt
     3781 tgtatgttag atcagaccaa atttcttcaa gcctttttca attccatctt catcaactct
     3841 tgctgtctga tatggtgcga tttctaataa ttctggtaca gaattaccca ttgcaacacc
     3901 atattttaca tatttgatca tatcgatatc attggcactg tcaccaaatg cataagaatc
     3961 ttctcttgaa attcctgtat gatcaaggat cacttctatt cccttcgcct tattaacacc
     4021 taatggtaca aattctactg aatttacagg ctcatgatat gttcctttgt atttgtgtcc
     4081 aaggatctct tcgatctctg gtttctttga aagctccata tgatggtatg tccattttgg
     4141 aacatccact gcttcattta atggatataa tgttgtttta cagtctgctt ctgttttatt
     4201 cttcatcgca atataataat catctgtgtt ctccggatca taatatccat atttttctcc
     4261 ttctaagatg attccgatct tctgtttctt tccccagtcg atcactgtct gtacttcttc
     4321 tggtgtcatc agttcctgat gcaggatctt gccttcatac tctacataag ttcctgcccc
     4381 taagatcact ccatcaaatc ccatatggaa atattcttct ggaatcattc ccttgcttct
     4441 tcccgtgcac ataacgatct tgtgctcatt tgcgatcaac cgattcaatg cattaactgt
     4501 tgattctgga attccgattc tagaatcata taatgttcca tcgatgtcaa aaaatattat
     4561 cttactcatt gtttcccttg ttctccatta aatattctct atctgccaat caattggcgc
     4621 tagtccatgt tctttcaaaa attcatttgc ctgactaaaa tgatggcatc cgaaaaatcc
     4681 tctatatgct gataacgggc ttgggtgcgg tgcttctaat atcaaatgat tcggattatc
     4741 aagtaatgct ttcttactct gtgctggacg tccccaaagc ataaatacga tcggacggtc
     4801 ctgttgattc aacactctga tcgccgcatc agtaaactcc tcccatccaa tacctctgtg
     4861 agaattcgca cgatgtgcct gtacagtcaa tactgtgttt aacatcataa caccctggtc
     4921 tgcccatttc tttagatatc cattattcgg aatatagcat ccaagatctt cgttcaattc
     4981 tttataaata ttaaccaatg atgggggcgc ttctactcca ggttttacag aaaatgacaa
     5041 tccatgtgcc tgtccatctc catgatatgg atcctgccct aagatcacaa cttttacatc
     5101 ctttactgga gtaaaatgaa atgcattaaa gatatcgtca gctggtggaa agatcagctt
     5161 agtgtgatat tcatccataa ctttttggta taatttccta taatatggct ttttgtattc
     5221 ttcttttaaa taatctgccc agtcattcgt aattggcggc atttttcgac ctccttttta
     5281 aaatcttttc ccattatatc ataatttgta gtaaaataaa attcgatatg aatatgaaaa
     5341 gaaaaggagg gtattatttt gaagcaaatg aaaagcttac tggaaaatcc ccacggattt
     5401 tctgtctcac ttgtatttga cggactgctt gtcggaatct ttgccggatg catttctgtc
     5461 atttaccgcc tgcttttaaa taacgctgaa aaactattat ttacgatcat tgcatttact
     5521 aaaacaaggc ctttctggat agctgtctgg tttatcgttt tgatcgttat gggactgatc
     5581 gttggatggc tcatgtcttg ggaaggcatg gcttctggaa gtggtatccc acaggttcaa
     5641 ggagagctga aaggttattt aaatcagaac tggcaccgtg ttctttgttc taagatcatt
     5701 ggtggaactt tatgtattct tggaggttta tctctgggac gcgaaggccc ttccgtacag
     5761 cttggtgcca tggttgcaaa gggtattgct aagattacaa aaaaatctca aaccaaagaa
     5821 cgctatatga tgacttgtgg tgcaggtgcc ggtcttgccg ctgcctttaa tgcaccgctt
     5881 gcaggtgtga tgttttcact ggaagaatta cagaagaact ttaacagctc tatgttagtc
     5941 tgcatcatct ctggatgtgt aacttccgat tttatttcaa aaaatgtctt tggattatct
     6001 ccagtttttg attttcattt aaaggctgct cttcctcttg tacattattg gatgttaata
     6061 cttcttggaa tcctgcttgg gctttgtggt gctttttata actttatcat gttgaaagga
     6121 caggatttat ttggagcaat gaaaaaaatc ccagcaaaat accgtatcgt atttcctttt
     6181 gtagtatctg ggatcgtttg ttacacgctg ccttctatct tagcaggtgg acatgctatg
     6241 atctctctga tcacaggaca tactttactt gtctctacga tgttattatt attagttgct
     6301 aagtttttct tctccgcctt ctgttttggt tccggggctc ctggtggaat cttctttcca
     6361 ctgttgattc tcggtgcata ccttggggcg atctatggaa cactggtgat tcaggcaagt
     6421 ggaattcctt cttattacct agtgaacttt attacgatct ccatggctgg attctttaca
     6481 gcgatcgtac gtgcaccgat cacaggtatc ctgctgatcg ctgagatgac tggtacattt
     6541 gaacacttcc tttcactggc agttgtttgt atcatcagct atcttgttgc acatctgtta
     6601 aagagtgaac caatttacga aagtctgcta ggacgaattc ttgctaaaaa tggctttaaa
     6661 gaaagcgaag atgctgatca taaggttctt cgtggatttg ctgtcggcac tggttccatt
     6721 gcagccaaac agcttgtaaa agatattcca tggccagaac attgcttgat cgttacttta
     6781 aatcgtggtg atgaagagat cattgctcga ggaagcactg aaattcatgt tggcgatacg
     6841 atcattgcct tgatcgacga taattatctt ggaatggtca cggaaagcat tcagctaatc
     6901 tgcggtgaga tcattcctga ataattttat attcaattta cttttattga gaaagttttc
     6961 agtttttata tgttgcgatt gactaacctt acttttgact aataaaatat aagaaaaaga
     7021 tacatcatat aagttcgaag gagggattat catgcaacca ttaacaaaag tcatgcaggg
     7081 agcgacttgc actgtcaagt gggcattagg cttacctgaa actgttgaaa gctccacgaa
     7141 ctgaagattg aacagggaag taaactgcaa gttgtttgta aaagcagcga ttatatgatc
     7201 atcggcgttg gatcaaaacg gtatgctgtt gggaatgaaa ttgctggaag aattcaggtt
     7261 tagataatta taaaaataaa taagaacgag aaaatctcca tataacttga ttttctcgtt
     7321 cttattttgt ttatatttgc atcataaatc aactcaccaa ccttgtaacc tatattcatt
     7381 tatatnnnnn nnnnncagga tatcccctgc ctgttgattc tctcaagtcc atcgacatca
     7441 tgatctgcat gtcttccttt gataattcaa aatcaaagat atccgcattc tgtatgatcc
     7501 gttctttgtg aacagatttt ggaaggctta cgatccctct ctgcaaatgc cagcgtaaga
     7561 tgatctgtgc aggtgtttta ttgtattttt tagcaatagc tacgatttct ggttctgaaa
     7621 gctgttttcc tttgcctaga ggtccccatg cttctacaac aatatcatgt tcctggcaat
     7681 aatttacggt ctcttcctgc acaaggaatg ggcagatttc aatctgatca accgctggtg
     7741 caatgttagc tgttacttct aattgtttta aatgttttgg acgaaaattg ctgactccta
     7801 tattcttgat cagtccttgt tcataatatt tctcaagaac tttccaggaa cctttgacat
     7861 cattgatcgg ccagtgtaat aaatacatat ccacatattc aagtccaagc tcttttaatg
     7921 tcttttcaaa tgcagcttct tgttttcctt ctaccatatc ttcgtaccac atcttacttg
     7981 tcacaaagat ttcctctctt gggatctcgc aatctctgac agctgctcca accagcgctt
     8041 cattctcgta ataggctgct gtgtctatat gacgatagcc tgatgataat gcatcaagac
     8101 atgcctgata tgcttcgtcc ccgcggctgt cccaacttcc gagtgccatc actggtattt
     8161 catttccatt gtttaattta aacgttgaac ttacattcat gtcttcctcc ttgtatctgt
     8221 tatctttgtc tttatactac aactggaacc atcgtccctt gatagttcct attataatcg
     8281 atttcttgct atttataaat gcaaatgata aaatcaacat aacaggcaat ggaaaggagc
     8341 gtgtacagaa atggatagga agctgaaact gcccatagga attgataatt ttgaaaaaat
     8401 tcgaaaagaa aatttctatt atgtagataa aacaaagctg atcgaacaac ttcttgaacg
     8461 atggggagaa gtgaatctat ttacaagacc acgccgtttc ggaaaaacat tgaatatgag
     8521 tatgctgaag tat
//
LOCUS       DAOMUE010000054         8416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2170_length_8416_cov_2.536299, whole genome shotgun sequence.
ACCESSION   DAOMUE010000054 DAOMUE010000000
VERSION     DAOMUE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2170_length_8416_cov_2.536299"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..415)
                     /locus_tag="PLC71_03945"
     CDS             complement(116..415)
                     /locus_tag="PLC71_03945"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM01828.1"
                     /translation="MANEVKILVACGSGVATSTVAQEAVKKILADANIPGKILKGSMS
                     EIPLKQNDVDLILTTTNYRKPLEKPCISVFGLISGINEGKIKQSIIDECHKILGE"
     gene            complement(714..860)
                     /locus_tag="PLC71_03950"
     CDS             complement(714..860)
                     /locus_tag="PLC71_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01829.1"
                     /translation="MILVMEGDKQVAGIPVKIVKSTMSKIQSKQDDVDIVILEDLSEI
                     TKML"
     gene            1146..2126
                     /locus_tag="PLC71_03955"
     CDS             1146..2126
                     /locus_tag="PLC71_03955"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HRM01830.1"
                     /translation="MAKTDELMKKLFKHKEIFADLFNATLFNGKQIIKAEKLAELNTE
                     NIHVDESISSNVNPSILKRYRDLCMRQDDSVLQIILGCENQSEIDYSMPIRTMLYDAL
                     KYTEQQNNLELRKRKDGTYYHSKLRKDDKVLPVLTLIFYYSDKEWTAGKCIHDLIKWP
                     EEPEIKSIVPNYKLNLLWAYQINNIDKYKSDLQYILSMLKYKEEEKSLKQYISDNNDK
                     IQNMNQDSHNVAVALLNQNLPKLIDNQKGDFRMGENALQAIYDRGVNQGEVLHLIMQI
                     NKKILNNKNLDTIANELEEKKEDILPIYTIIKEHPDKSKEQIYAILKKES"
     assembly_gap    2173..2182
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2210..2995)
                     /locus_tag="PLC71_03960"
     CDS             complement(2210..2995)
                     /locus_tag="PLC71_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRM01831.1"
                     /translation="MAQEPKYPVQTVMKALELLNHLAKNTGNLGAGVSELSDALGIGK
                     STVHRLLDTLQYYGYIEKSEETNRYRLGWELYKVGLSVPAQNQLFNIDRTHLLELGKK
                     LNETIDYGTIKGKETIIISKMEYTSNGMNNSVSPGEYESIHATALGKVMISEMSEEEI
                     YKLFEDKEELPAYTPHTILTVSELIHEAEKVKKQGYGVNDEEYWIGMYCMAVPVRNYT
                     GKIVAAVSASIPVFRVDDKKKEFVLDELNRTAGQISKELGYRG"
     gene            complement(3212..3661)
                     /locus_tag="PLC71_03965"
     CDS             complement(3212..3661)
                     /locus_tag="PLC71_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HRM01832.1"
                     /translation="MIGKKVVHHMMMSEKDAHYVGGLVNGARIVVQWGDVGTELMVYA
                     DGDISLFLGYEKIEFTAPVYVGDFMEYVGWIEEVGNQSYKCKFEAWKVATMLDRTDAD
                     MEGIEHTAATACEPPILCATGTGSLYIAKKDQRGPQEESFKDLKHRA"
     gene            4120..4575
                     /locus_tag="PLC71_03970"
     CDS             4120..4575
                     /locus_tag="PLC71_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HRM01833.1"
                     /translation="MSVVMANALGTGIFCGLWQGIGSVLGLATWMGFVGCTSYFSAGC
                     GKTGFIKSMCSNYVGLLWGMCIIMSGNINSGILFGAIVTGAFSWLICYQSKIDLLSLV
                     PNTFMGGFSAFASGGDWKMLCICLVLGNILGVCCDYSGRFLAAKFGKDN"
     gene            4623..5831
                     /locus_tag="PLC71_03975"
     CDS             4623..5831
                     /locus_tag="PLC71_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM01834.1"
                     /translation="MSKVIKKAYAEEMKQGYNLRTAMEEASRCLLCHDAPCSKSCPAK
                     TDPAKFIRSLRFRNIKGAAETIRENNPLAGCCAEVCPYGQLCEQACSRTGIDKPIEIG
                     KLQKFLVEMEKREGMEFLHVEEKRDGKIACVGAGPASLACARELALFGYDVTIYEANE
                     KAGGVLTYGIVPSRLPQEVVDFDIATIEKLGVTFKFNETIDAARLEELKAEYDAVFVG
                     TGLWKSKVIDIPGKDLDGVVSAIDFLKDARLNKENMTLGDTVLVIGGGDVAMDCVTTA
                     KQLGAKATIVYRRTIEEAPADIDEVMAVQSMGIPIIQEFAPGEIIGSKGHVTAMKFNG
                     RDNESELTMKADQVVFAIGQAACDTTKDIKAGGKVFTGGDMVNGGKTVVQAVAEGKDA
                     AAMIAAYLNK"
     gene            5848..>6527
                     /locus_tag="PLC71_03980"
     CDS             5848..>6527
                     /locus_tag="PLC71_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012451722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRM01835.1"
                     /translation="MSLKKDLSIDFLGVKCENPFFLSSSPVGSNYEMCAKALEAGWGG
                     IYYKTIGIFIPDECSPRFDITTKEGTPWVGFKNMEQISDKPLEVNLEYMRRLKQDYPN
                     KVIVASIMGSNDEEWTTLAKAVTEAGVDMIECNFSCPQMTSHAMGSDVGQNPELVKHY
                     CEVVTAATHLPVIAKMTPNIGNMEIPAIASMEGGAAGIAAINTVKAITNIDIENITAM
                     PVVNGKSS"
     assembly_gap    6528..6537
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6538..7100
                     /locus_tag="PLC71_03985"
     CDS             <6538..7100
                     /locus_tag="PLC71_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM01836.1"
                     /translation="AAVKPIALRFITQMKQHPDLVNVPITGVGGIETWRDALEFILVG
                     ASNLQVTTAVMQYGYRIVEDMISGLSHYMEDNGIEKLSDLVGLALPNIVPAEDLDRSF
                     KLLPKFDEDACAGCGRCYVSCFDGGHQAIDWDEEARRPRLNTDKCVGCHLCLNVCPVM
                     DCITPGEIVIKPGREEHEIKIKTKYE"
     gene            7134..>8416
                     /gene="hydA"
                     /locus_tag="PLC71_03990"
     CDS             7134..>8416
                     /gene="hydA"
                     /locus_tag="PLC71_03990"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HRM01837.1"
                     /translation="MKEFDLIIKNGTVVTSTESFLCDVAVKDGKIAAMAQNIEADAKE
                     IYDAAGKLVLPGALDAHTHMAMPFGGTVSADSYMAGTRAAACGGVTTIFDYPVQHKGE
                     TIRGLVNSKKEVLEKEACVDYAMHCCITDLNEGEILEEMEKAVAEGITSFKCFLVYKK
                     EGMMVDDGMLARLMLRAKELGAMINIHAENPDLIDYYTEKFLSEGKTSAWYHYMSRPE
                     FVEAEADKRAVHWAKHLDAPLYLVHMADKEGLEACIEAKEEGADVFVETCPQYLLLDD
                     SVYAKPDFQGAVYVCAPPIRKKEDQDCLWEALSREDIQTVATDQCSFTLAQKALGKDD
                     FTKIPGGLPGVQTRGTLLYTYGVKEGRITQEQMCKLLSENPAKLYGVYPQKGVLAPES
                     DADIVVLDPEKESVISAATHAYNTDNNPFEGFRLR"
BASE COUNT         2576 a         1535 c         1537 g         2748 t
ORIGIN      
        1 gaacagacca atgatgatca atacgattgg cagcataacc gctgctccta aatctacaat
       61 tcctttaaaa aagctaaata cagcatccat ttttattcct cctatttatt tgatcttatt
      121 ctccaagaat tttatgacat tcatcaatga tactctgttt aatcttgcct tcattgattc
      181 ctgaaatcag accaaataca ctaatgcaag gtttttctaa tggttttctg taatttgttg
      241 ttgttaagat taaatctaca tcattttgtt ttaaaggaat ctcactcatg cttcctttta
      301 aaatttttcc tgggatgtta gcatctgcca aaatcttttt tactgcttcc tgtgctactg
      361 ttgatgttgc tactccactt ccacatgcca ctaagatttt tacttcattt gccataattt
      421 ttatcctcca aatatttctc tgttggttgt atttcgattc gctactaatt catgaattaa
      481 cttatgctat tataatatat ctgacaaaaa aatcattcaa gccaagtttt tgtttgtcta
      541 tttttctcaa aatgtgctaa gtcaaattca gttcttgtac aaaatactca ttttttgcta
      601 atgaaatatc tggatttata taattttagc caaatgataa accttatata aattaatgct
      661 gtaaaacaaa aagaacacga aactagtagt aatcgttccg tgttcttttc gtattataac
      721 atttttgtaa tctcagagag atcttctaag atcacaatat ctacatcatc ctgttttgac
      781 tgaatttttg acattgtact tttaacgatc tttacaggaa ttcctgctac ttgtttgtca
      841 ccttccatga ctaagatcat atcttcattt aaaagtgctt ttcttatacc agaagatgta
      901 atatataact ggtttgtctc acgatcaaga attgataaat ttccttcaag tgtattattt
      961 ctttatctcg tcatttatgc tgactcaaat tttattattt tttagattta ttgctattga
     1021 cattttttgt aaatatatta taatattata aaattgtaaa atatttactt ttattttata
     1081 actcagttct tataaatcta tacacattgt ctatttctat catatctaac acaggaggat
     1141 tatctatggc taaaactgac gaacttatga aaaaattatt taaacataaa gaaatattcg
     1201 ctgatctctt taatgcaact ttatttaatg gaaaacaaat tataaaggct gaaaaattag
     1261 ccgaactcaa tactgaaaat attcatgttg atgaatctat ttctagcaat gtaaatccat
     1321 caattcttaa acgctatcgt gatctttgca tgcgtcaaga tgacagtgtc ttacaaatca
     1381 tactaggatg tgaaaatcag agtgagattg attattctat gccaattcgc actatgctct
     1441 atgatgcttt aaaatacact gaacaacaaa acaacttaga actcaggaag cgaaaagatg
     1501 gaacatacta tcattctaaa ttaagaaaag atgataaagt tcttcctgta ttaactctta
     1561 ttttttatta tagtgataaa gaatggactg ctggaaaatg tattcatgat cttattaaat
     1621 ggccagaaga acctgaaatc aaatccattg ttccaaatta taaattgaat ttgttatggg
     1681 cttatcaaat aaacaatatt gacaagtaca aaagcgattt acagtatatt ctctccatgc
     1741 taaaatataa ggaagaagaa aaatctttaa aacaatacat ttctgacaat aatgataaaa
     1801 tacaaaatat gaatcaagac agccacaatg ttgctgttgc actactaaat caaaacttac
     1861 caaaattaat agataatcag aaaggagatt ttcgtatggg ggaaaatgca ttgcaagcaa
     1921 tttatgatcg tggtgttaat caaggtgagg tcttacattt gattatgcaa atcaacaaaa
     1981 aaatccttaa caacaaaaat ttagatacta ttgcaaatga attagaggaa aaaaaagagg
     2041 atattcttcc tatctatact atcatcaaag aacatccaga taaatccaaa gaacaaatat
     2101 atgcaatttt aaaaaaagaa tcctaatata aaaagccgat agaatcatac aaaataaaat
     2161 atgatcccat cgnnnnnnnn nntttcatct catctacacc aatcactatc tatccccgat
     2221 acccaagctc cttagatatc tgcccagcag tcctattaag ttcatccaac acaaattcct
     2281 tcttcttatc atccacacga aataccggaa tacttgcact gactgctgcc acgatctttc
     2341 ctgtgtaatt tcttacaggc actgccatac aatacatccc gatccagtat tcttcgtcat
     2401 tgaccccata tccctgtttc ttcactttct ctgcctcatg gatcagctca ctgacagtta
     2461 agatcgtatg tggtgtatat gctggaagtt cttctttatc ttcgaataac ttataaatct
     2521 cttcctcgct catctcactg atcatcactt ttccaagtgc tgttgcatga atggattcat
     2581 actcgcccgg gcttacactg ttgttcatgc cattgctcgt atattccatc ttggaaataa
     2641 tgatcgtttc ttttcctttg attgttccat aatcgatcgt ttcgtttaat ttctttccaa
     2701 gttctaataa atgtgtacgg tcaatattaa ataactggtt ctgtgctgga actgacagcc
     2761 ctactttata taattcccaa ccaagacgat atctgtttgt ttcttcactc ttttcaatat
     2821 aaccataata ctgcagtgta tctaataaac gatggactgt actctttccg attcctagtg
     2881 catcgcttaa ttcactgact cctgctccaa gattccctgt attctttgca agatgattta
     2941 acaattccag tgctttcatt accgtctgca ctggatattt tggttcctgt gccatgactt
     3001 tcctcccgtt tatctgtttt tatatcctct tttgatcact atcattatat caaaccccgt
     3061 cttcactatg caaaatattt ttcttctatc tcgaactttt ttctaaattt tgaaacactt
     3121 catatataat cgaaaaaaga gacggtattt tgactaaatc cgtctcttga tcttttctct
     3181 ttcttctata tataactctc gatctaaaat cttatgcacg atgtttcaag tctttgaatg
     3241 attcttcctg tggtcctctc tggtctttct tcgcaatata taaacttcct gttcctgttg
     3301 cgcaaagaat tggtggttca catgctgttg cagctgtatg ctcgattcct tccatatccg
     3361 catctgtacg atcaagcatt gttgctacct tccatgcttc aaacttgcat ttgtaagact
     3421 ggtttcctac ttcttcgatc catcctacat attccataaa gtcgcctaca taaactggtg
     3481 ctgtaaattc gattttctca tatcctaaga acaaactaat atctccgtcc gcatatacca
     3541 tcagttctgt tcctacatcg ccccactgaa caacgattct tgctccatta acgagtccac
     3601 ctacatagtg agcatctttc tcactcatca tcatgtgatg tacaactttc tttccgatca
     3661 tagctcttac ctccattttc tgtgttctat tattcagaat cattttctaa atgttacttt
     3721 gtactgtgat gttaacattg gattgtattt ttgtcaatac atttttgtat tttttctgtt
     3781 atttataatt cctttctatt atatagaaaa tttaattgct atttttctaa tgaagcaaga
     3841 aatactttcc actaactgga gagttgtttc tgtctatttt ttataagtaa tgcacaattt
     3901 ttctgcaagt tattaaacat attgtctaaa tatcgtcgtt tttttgtcaa tctttttcca
     3961 attattgtat ttataaacaa tctgttacgt gtaaactttt ttctttttct tgatattttt
     4021 tctcattttc agaaaataag ctctttacat tccatctcat gtatccctta aaatacaatc
     4081 atagtcattc gtggagaaaa ataaagaaag gagtttcata tgagtgtagt tatggcaaat
     4141 gcacttggca ctggaatttt ctgcggactc tggcaaggta tcggttcagt tcttggactt
     4201 gctacttgga tgggcttcgt cggctgtact tcttatttct cagccggatg tggaaaaacc
     4261 ggttttatta aatccatgtg cagtaactat gttggtcttc tctggggtat gtgtattatc
     4321 atgtcaggaa atatcaacag tggcattttg tttggtgcga tcgtaactgg agctttctcc
     4381 tggctgatct gctatcaatc aaaaatcgat ctattatcct tggttcctaa cactttcatg
     4441 ggcggttttt ctgcttttgc cagtggcgga gattggaaaa tgctttgcat ctgccttgtc
     4501 ttaggaaata ttctcggtgt gtgctgcgat tattctggaa gatttctggc agcaaaattt
     4561 ggtaaagata attaattaag aaataagagt gaaacaaatt ttcaaaggag cgtgaaataa
     4621 agatgagtaa agtaattaaa aaagcttacg cagaagaaat gaaacagggc tacaatttaa
     4681 gaacagccat ggaagaagca tccagatgtc ttctctgcca tgatgcccct tgcagcaaga
     4741 gctgcccagc aaaaacggat ccagcaaaat tcatccgttc cctgcgtttt agaaatatta
     4801 aaggtgctgc tgaaacaatc cgtgaaaaca atccattagc tggttgttgc gcagaagtct
     4861 gtccttatgg tcagctgtgt gaacaggctt gcagccgtac tggaattgat aaaccgatcg
     4921 aaatcggaaa gcttcagaaa ttcttagttg aaatggaaaa acgcgagggt atggaattcc
     4981 ttcatgtaga agaaaaacgt gatggaaaga tcgcctgtgt tggagctggt cctgcttctt
     5041 tagcttgcgc aagagagtta gcattatttg gatatgatgt aacaatctat gaagcaaatg
     5101 aaaaagctgg tggtgtttta acatacggaa tcgttccttc cagattacct caggaagttg
     5161 ttgatttcga tattgcaaca atcgaaaaac ttggtgtaac atttaaattt aatgaaacga
     5221 tcgatgctgc acgcttagaa gaattaaaag ctgagtatga tgcagtcttc gttggaacag
     5281 gattatggaa atctaaagtc attgatatcc caggaaaaga tcttgatggt gttgtaagtg
     5341 cgatcgattt cttaaaagat gcccgcttaa ataaagaaaa catgactctt ggagatactg
     5401 ttttagtgat cggtggtggt gacgttgcca tggactgtgt aacaacagca aaacagctgg
     5461 gtgcaaaagc tacaatcgta tatagaagaa caattgaaga agcacctgct gatattgatg
     5521 aagttatggc tgtacagagc atgggaattc ctatcattca ggaatttgca ccaggtgaga
     5581 tcatcggtag taaaggtcat gtaactgcaa tgaaatttaa tggacgtgac aatgaatccg
     5641 aacttaccat gaaagctgat caggttgtat ttgctattgg acaggctgct tgtgacacta
     5701 caaaagatat caaagctggc ggaaaagtat ttactggtgg agatatggta aatggtggaa
     5761 agactgttgt acaggctgtt gctgaaggta aagatgctgc tgctatgatc gctgcatatc
     5821 tgaacaaata gaaaggaggc attgattatg tcattaaaga aagatttatc tattgatttt
     5881 ttaggagtta aatgtgaaaa cccattcttc ctgtcttctt ctccagtagg aagtaactat
     5941 gaaatgtgtg caaaagccct tgaagctgga tggggtggaa tttattataa aacaattggt
     6001 atctttatcc cagacgagtg ttctcctcgt tttgatatca ctacaaaaga aggaactcct
     6061 tgggttggtt tcaagaatat ggagcagatt tctgacaagc cattagaagt gaacttggaa
     6121 tatatgcgcc gcttaaaaca ggattatcca aacaaagtca tcgttgcaag tatcatggga
     6181 agtaatgatg aagaatggac aacacttgca aaagctgtta cagaagctgg tgttgacatg
     6241 atcgagtgta acttctcttg tcctcagatg acatctcatg ccatgggatc tgacgttgga
     6301 cagaatccag agttagtaaa acattattgt gaagttgtta cagctgctac acatcttcct
     6361 gtcatcgcta aaatgactcc aaacattgga aatatggaaa ttcctgcgat tgcttctatg
     6421 gaaggtggtg ctgctggtat cgctgccatc aatacagtga aagcgatcac aaatattgat
     6481 atcgaaaata tcactgctat gcctgttgta aacggtaaat cttctatnnn nnnnnnngag
     6541 ctgcagttaa acctattgct cttcgtttca ttacacagat gaaacagcat ccagatctgg
     6601 ttaatgtgcc tatcactggt gttggtggaa tcgaaacttg gagagatgcc ttagagttta
     6661 tccttgttgg tgcttctaac cttcaggtaa ctacggcagt tatgcagtat ggataccgca
     6721 tcgttgaaga catgatcagc ggattatctc attacatgga agataatggt attgaaaaac
     6781 tttctgatct ggtaggtctt gcccttccta atattgttcc tgccgaagat cttgatcgaa
     6841 gtttcaaact gcttcctaaa tttgatgaag atgcttgtgc tggatgtggt agatgctatg
     6901 tatcatgctt tgatggtgga catcaggcaa ttgactggga tgaggaagct cgtcgtccaa
     6961 gattaaatac agataaatgt gttggatgtc atctatgcct gaatgtctgt cctgttatgg
     7021 attgtatcac tcctggtgag atcgttatta aacctggacg tgaagaacac gaaatcaaaa
     7081 tcaaaactaa gtacgaataa gaaatatcac attgataagg aggtcttagt aatatgaaag
     7141 aattcgattt gatcattaaa aacggaaccg tcgtaacctc taccgaaagt tttttatgcg
     7201 atgttgcagt gaaagacgga aagatcgctg ccatggcaca aaatattgaa gcagatgcga
     7261 aagaaattta tgatgccgct ggtaaattag ttcttcctgg tgctttagat gcacatacac
     7321 atatggctat gccatttggt ggaactgttt ccgctgacag ttacatggca ggtactcgtg
     7381 ctgctgcctg tggtggtgtc acaacgattt ttgactatcc tgtacagcac aaaggagaaa
     7441 cgatccgcgg attagttaac tccaagaaag aagttttaga gaaagaagcc tgcgtagact
     7501 atgcaatgca ctgctgcatc actgatttaa atgaaggtga gatccttgaa gaaatggaaa
     7561 aggcagttgc tgaaggtatc acaagtttca aatgcttcct tgtttacaaa aaagaaggaa
     7621 tgatggtaga tgacggaatg cttgcaagac tgatgcttcg tgcaaaagaa cttggagcga
     7681 tgatcaatat ccacgctgaa aaccctgatc tgatcgatta ttatacagag aaattcttaa
     7741 gtgaaggtaa aacaagcgct tggtatcatt acatgagccg tcctgaattc gtagaagctg
     7801 aggccgacaa acgtgctgtt cactgggcta agcatttaga tgctccttta tatctggttc
     7861 atatggcaga taaagaaggt ctggaagctt gtatcgaagc caaagaagaa ggtgctgacg
     7921 tattcgttga aacatgtcca cagtatctgc tacttgatga tagtgtgtat gcgaaaccag
     7981 attttcaggg agctgtttat gtctgtgcac ctccgattcg caaaaaagaa gatcaggact
     8041 gtctgtggga agcgttgtca cgagaagata tccagacagt agctacggat cagtgcagtt
     8101 ttacacttgc gcagaaggct ctgggcaaag atgattttac aaagattccg ggaggacttc
     8161 cgggagttca gacacgtgga acattacttt atacatatgg ggtaaaagaa ggcaggatta
     8221 cgcaggaaca gatgtgtaaa cttttatccg aaaatccggc gaaactgtat ggcgtttatc
     8281 ctcagaaagg tgtgcttgca ccagaaagtg atgcagacat tgtagtactg gatccggaaa
     8341 aagaaagtgt gatatctgct gccacacatg cttataacac tgataataat ccatttgaag
     8401 gattcagact gcgctg
//
LOCUS       DAOMUE010000055         8375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2191_length_8375_cov_2.877284, whole genome shotgun sequence.
ACCESSION   DAOMUE010000055 DAOMUE010000000
VERSION     DAOMUE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2191_length_8375_cov_2.877284"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLC71_03995"
     CDS             <1..258
                     /locus_tag="PLC71_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HRM01838.1"
                     /translation="LCDDEELIVTTDIFGGSVNNEFMKYLSKSNIHLIAGVNLPLLFE
                     LIMNLESENTVQMIENAIQNAKEQLQYCNPLIQCSITEENF"
     gene            280..1584
                     /gene="rpoN"
                     /locus_tag="PLC71_04000"
     CDS             280..1584
                     /gene="rpoN"
                     /locus_tag="PLC71_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRM01839.1"
                     /translation="MNFSPKTTLVQTQTLSQTQLQSLKILSLDNFELNNFLQNEYIEN
                     PLLDYNENSNLPSWNQYVSYSSAKEDEDIYKEIPDFSENGIKEFLLDQLNQRNFSDLQ
                     WTAMQTMIDCLDDCGYFKLSLKDFSQWFGIDISTVKYCLDILSSLEPLGIFSKDLPHC
                     LLRQLEARQELTPELNQIIRYHLDDIAAGKINTITQKLSMSTQDVRKCIAKIRELNPR
                     PISGFLTNSTDYIVPDILIDYQDSSWIIRLNDQWIGNYSLCDYYLNMMHTSQSPELKE
                     YFRKKYNRVQLIMHSIEQRRETILKITSAVLERQKDYFTGNSTLKPMTLADIASDISM
                     HTSTISRGIKNKYLQYPFGVVYLKDLFTSSAGKKDNNSPITVKKQLKAIIASENPNKP
                     YSDSTLSNLLKQKGIKISRRTVAKYREEIGIKSTYNRKIYNE"
     gene            complement(1990..2328)
                     /locus_tag="PLC71_04005"
                     /pseudo
     CDS             complement(1990..2328)
                     /locus_tag="PLC71_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2468..5947)
                     /gene="nifJ"
                     /locus_tag="PLC71_04010"
     CDS             complement(2468..5947)
                     /gene="nifJ"
                     /locus_tag="PLC71_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009264164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRM01840.1"
                     /translation="MARKMKTMDGNTAAAHVSYAFTEAAAIYPITPSSPMAESTDQWA
                     TQGKKNIFGHPVKLVEMQSEGGAAGTVHGSLAAGALTTTYTASQGLLLMIPNMYKIAG
                     EQMPCVFDVSARALASHALNIFGDHSDVYACRQTGFAMLCSSSVQEVMDLGAVAHLAT
                     IKGRVPVLHFFDGFRTSHEIQKIEMWDYDDLADMVDYEAIDKFRAECLNPEHPVLRGS
                     AQNPDIFFQAREAANSTYNAFPAIVEDYMNQVNAKIGTDYKLFNYYGAEDAESVIIAM
                     GSVCETIDETIDYLMKQGKKVGVVKVRLYRPFVAEKLVEAIPDTAKVINVLDRSKEPG
                     AREPLYLDVVNALRGTKFESCTINGGRYGLGSKDTTPADIIATFENVDKNEFTLSIVD
                     DVTNLSLERGETPVTAPDSIVACKFWGLGADGTVGANKNSIKIIGDHTDKYAQAYFDY
                     DSKKSGGVTMSHLRFGDDPIKSTYLINKADFVACHCAPYLTKYNMVQDLKDGGTFLLN
                     CSWTPEEVGEHLPGQVKAYLAKHNIKFYIIDGIKIGKEIGLGGRINTVLQSAFFKLSG
                     IIPEEDAIKYMKDAATASYSKKGEAIVKMNHDAIDAGAKAIVEVPVPAEWADAETESL
                     FVEAKGDNKPLVDYINNVQNYVNGQEGNALKVSDLVPYVDGHMPLGASAFEKRGVAVD
                     VPSWNPDNCIQCNFCAYVCPHATIRPVAMTEEEAAAAPAATKTADMTGMPGYKFAMTV
                     TVLDCLGCGSCVNICPGKKGEKALTMQGLDSQRDQQEVYDYGQKLPAKEEVLAKFKAD
                     SVKGSQFRQPMLEFSGACAGCGETPYAKLITQLFGERMYIANATGCSSIWGGSAPATP
                     YTTNAKGQGPAWANSLFEDNAEFGLGMFTGQNTLREQTKEKVVALAESTDNADVKAAA
                     AEYLDTYADGSKNGAATDKLVAALEACGCDAAKEILAKQKTAYDIGFGGVDHVLASGE
                     DVNVFVFDTEVYSNTGGQASKSTNLGAIAQFQAAGKGTKKKDLAAIAMSYGYVYVAQI
                     AMGADYNQTVKAIAEAEAYPGPSLVIGYAPCINHGIKGGMKIAQTEEKKAVTSGYWNL
                     FRFNPALAAEGKNPFHLDSKAPTTEYEDFIMGEVRYNALSRQNPERAKELFAEAKANA
                     EAKYNRLKEMSDK"
     gene            complement(6212..7174)
                     /gene="mltG"
                     /locus_tag="PLC71_04015"
     CDS             complement(6212..7174)
                     /gene="mltG"
                     /locus_tag="PLC71_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRM01841.1"
                     /translation="MDLYLDFDHNSGRRRRGRRRGRRNRSRVPFVIGVIILLVVIVAG
                     GIFAGRKYMAYRQQKAEEARKLAEARRVVTVMIPEGYSIDMIAKRLEKQGVFKADEFI
                     KAAKNTNQYKNDFIKDIDPKKGTKYKLEGYLYPDTYKIYKSSKPEDLIQKMLDNFDKK
                     YSALAKSYKGKRSMAEIMTIASMIEREASNMSERPMIAGVIENRLAAKMRLQIDPTVL
                     YTTTNGLYNAKKVYYKDLKVKTVYNTYVMKGLPAGPICNPSDTAIKAAMHPKKHDYLY
                     YRTDGSKKGTHVFTKTFDEHKNAKSTSTKDKNSTNSTNTTNSKS"
     gene            7443..7928
                     /locus_tag="PLC71_04020"
     CDS             7443..7928
                     /locus_tag="PLC71_04020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM01842.1"
                     /translation="MANPIVTITMENGDVMKAELYPEIAPITVENFVNLVKDGFYDGL
                     TFHRIINGFMIQGGCPLGTGTGGPGHTIKGEFSQNGVINNLKHTPGVLSMARSMMPDS
                     AGSQFFIMHKAAPHLDGQYAAFGKIIEGMDVVNKIAEVATDYSDKPLEPQVMKTVTVE
                     E"
     gene            8022..>8375
                     /locus_tag="PLC71_04025"
     CDS             8022..>8375
                     /locus_tag="PLC71_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRM01843.1"
                     /translation="MSENNKLIFLDVDATLYSKEQRLVPESTIKAVHEAQENGHKVLI
                     NTGRPLVYFEKEILDIGCDGYLCSNGVHILLDGETIYHKTVPETVVEQIKRICEENNI
                     YGTFEGEKCSYFRDHN"
BASE COUNT         2433 a         1710 c         1477 g         2755 t
ORIGIN      
        1 ttatgtgatg acgaagaatt aattgtaaca acagatattt tcggcggaag cgtcaataat
       61 gaatttatga aatatctttc aaaatcaaat attcacctga ttgctggagt aaatcttccc
      121 ttactttttg aactaattat gaatcttgaa tcggaaaaca cagttcaaat gattgaaaat
      181 gcaatacaaa atgccaaaga acaattgcaa tactgtaatc ctttaataca atgttctatt
      241 acagaagaaa acttttagta agatttggag tgtgatgaaa tgaatttcag ccctaaaact
      301 actttagtgc aaacccaaac attatctcaa acacaactac agtctttaaa aatactgtct
      361 ttggataatt ttgagttaaa taacttcctt caaaatgaat atattgaaaa tcctttgcta
      421 gattataatg aaaattcaaa tcttccttca tggaatcaat atgtttccta ttcctccgct
      481 aaagaagacg aggatattta caaagaaatc cctgactttt cggagaatgg aatcaaagaa
      541 ttccttctgg atcagttaaa ccaaagaaat ttttcagatt tacagtggac agcaatgcag
      601 accatgattg actgtctaga tgactgtggt tattttaaat tgtcgttaaa ggatttcagt
      661 caatggtttg gtattgatat atctactgta aaatactgct tggacatact atcctctttg
      721 gaaccattag ggattttttc aaaagatctt ccacactgtc ttttacgtca gttagaagca
      781 aggcaggaac tgacaccgga actcaatcaa atcatccgtt atcatctgga tgacattgct
      841 gctggaaaaa ttaatactat cacacaaaaa ctttctatgt ctacacagga tgtccgaaaa
      901 tgtattgcaa aaatcagaga attaaatcca cgcccaatct ctggatttct tactaattcc
      961 acagattata ttgtgccaga tattcttata gattatcaag acagcagttg gattattaga
     1021 ttaaacgatc agtggattgg aaattattcc ctctgtgact attatttaaa tatgatgcat
     1081 acttcgcaga gtccggagtt aaaagaatat ttccgtaaaa aatacaatcg tgtgcaattg
     1141 attatgcata gtattgaaca acgccgagaa acaatcttaa aaattacatc tgctgtttta
     1201 gagcgccaaa aagactattt tactggaaat agcacattaa aacctatgac acttgccgat
     1261 atcgcttctg atatttcgat gcacacatcc accatcagcc gtggaatcaa aaataaatat
     1321 ctccaatacc cttttggagt ggtttatttg aaagatttat tcacatcttc tgctggaaaa
     1381 aaggataaca acagcccaat taccgtaaaa aaacaattga aggctataat cgcttctgaa
     1441 aaccctaaca aaccctacag tgattcgaca ttatcaaatt tattaaaaca aaaaggaatc
     1501 aaaatttcca gacgtactgt cgcaaaatat agagaagaga taggaattaa aagtacatat
     1561 aaccgaaaaa tatataacga atagaaaggc cgcatctgga atccgcaatg cagaaatggt
     1621 acgagaaatg ataaacgctg gagtagacca attcggaatc agtcattcca gcgtcatgaa
     1681 aatcattttg gaattagctg caattgaaaa atcatcgtaa tctttctaat attactgcta
     1741 tgttacgaaa ctgtattcac agcatttccg ataccgtagc aacatgatgc cttcatcctt
     1801 gctacggtaa catcacaatt atcattgctt ttgataattt cagtcaattt ttctaaatag
     1861 cctactgcat ccaattggga acttccaaca tattctgact acctgagtac agaatacaat
     1921 cctatgtcaa caaaaaacag ttaacactag acgatctttg tctcatataa aaaattttca
     1981 aagaacctct taatcacgta cttttatgtg tacttctctt aattgctgtt ctgtaatctc
     2041 gcttggggct ccgtacatta aatcctgtgc agttccgctc attgggaaat cattgatata
     2101 gcagaagcgg tatgcatttt tctccagtaa atccaaacgg cttcccagtt catctctgat
     2161 ctgccccgca tagatgtagg ctgccttttc cggcgctgcc gatcttgcgt ctgtcgtata
     2221 tttctgagca acattgtatt ttaatacatc tgctccaaag gttgatcctg ccccctgttt
     2281 tgtaccgctt ccaacttcct cattgtcaaa gacactgcac atcgtcacat aatccttaga
     2341 gcttcacgat ttattaatcg ttaaagcgac aaccccttat ttttatgcat aaaaaatccc
     2401 ctctgtgatt gctcacaaag gggattttga tttattagat taagatcatc taaacaatag
     2461 gtaatgccta tttatcagac atttctttta atctgttgta tttagcttct gcgtttgctt
     2521 tagcttctgc gaacagttct ttagctcttt cagggttctg acgagataaa gcgttgtaac
     2581 gaacttctcc catgatgaag tcttcgtatt ctgttgtagg agctttagaa tctaagtgga
     2641 atggattctt tccttcagca gctaatgctg ggttgaatct gaacagattc cagtatccag
     2701 aagtaacagc tttcttttct tctgtctgag caatcttcat tccacccttg ataccatggt
     2761 tgatacatgg agcgtatccg attactaatg atggtcctgg ataagcttca gcttcagcga
     2821 ttgctttaac tgtctggttg taatcagcac ccatagcgat ctgtgctaca tatacatatc
     2881 cgtagctcat tgcgattgct gcaaggtctt tcttcttagt tcctttacca gctgcctgga
     2941 actgagcgat tgcacctaag ttagtagatt tagatgcctg tccacctgtg ttagagtaaa
     3001 cttctgtatc gaatacgaat acgtttacgt cttctccaga tgctaataca tggtcaacac
     3061 caccgaatcc gatatcgtat gccgtcttct gcttagcaag gatttcttta gcagcgtcac
     3121 atccacaagc ttctaatgct gctactaatt tatctgtagc tgctccgttc ttgcttccgt
     3181 ctgcatatgt gtctaagtat tcagctgctg ctgctttaac atctgcgtta tctgtagatt
     3241 cagctaatgc aacaactttt tcttttgtct gttctcttaa tgtattctgt cctgtgaaca
     3301 tacctaaacc aaactctgcg ttatcttcga acagtgagtt agcccaagct ggtccctgtc
     3361 ctttagcgtt tgttgtgtat ggtgtagcag gtgcagatcc accccagata gaagaacatc
     3421 ctgttgcgtt tgcgatgtac attctttcac cgaataactg agtgatcagt ttagcgtaag
     3481 gtgtttcacc acaaccagca catgctcctg agaactcaag cattggctgt ctgaactggc
     3541 ttcctttaac ggaatcagct ttgaatttag caagtacttc ttctttagct ggtaatttct
     3601 gaccatagtc ataaacttcc tgctgatctc tctgagaatc taatccctgc attgtaagag
     3661 ctttttcacc cttcttacct ggacagatgt ttacacaaga tccgcatcct aaacagtcaa
     3721 gaactgtaac tgtcatagcg aatttgtatc ctggcattcc agtcatatca gctgtcttag
     3781 tagctgctgg agctgctgct gcttcttctt ctgtcatagc tactggacgg attgttgcat
     3841 gaggacatac ataagcacag aagttacact ggatacagtt atctggattc caagaaggaa
     3901 cgtctactgc tactccacgt ttttcaaatg cagatgcacc taaaggcata tgtccatcta
     3961 cgtaaggtac taaatcgcta actttaagag cgttaccttc ctgtccgtta acgtagttct
     4021 gtacgttgtt gatgtaatca acaagaggtt tgttatctcc cttagcttca acgaataagc
     4081 tttctgtttc agcgtcagcc cattcagctg gaactggtac ttcaacgata gcttttgcac
     4141 cagcatcgat agcatcgtgg ttcatcttaa cgatagcttc acccttcttg ctgtaagaag
     4201 ctgttgcagc gtctttcata tatttgatag catcttcttc agggatgatt cctgataatt
     4261 tgaagaatgc agactgtaat acagtattga tacgtccacc aagtccgatt tctttaccga
     4321 tcttgatacc gtcaatgata tagaacttaa tgttgtgttt agctaagtaa gcttttacct
     4381 gtcctggaag atgttctcca acttcttctg gagtccagct gcagtttaac aggaatgttc
     4441 caccgtcttt taagtcctga accatgttat attttgttaa atatggtgca caatggcaag
     4501 caacaaagtc agctttgttg atcaggtatg ttgatttgat tgggtcatct ccgaaacgta
     4561 agtgggacat tgtaacacca ccggatttct tagaatcata atcaaagtat gcctgagcat
     4621 atttgtctgt atggtcacca atgatcttga tagagttctt gttagcacca acagtaccgt
     4681 cggctccaag tccccagaat ttacaagcta cgatgctgtc tggagctgta actggagttt
     4741 ctcctctttc aagagataag tttgtaacat catccacgat agataatgtg aattcgtttt
     4801 tatcaacgtt ttcgaatgta gcgatgatat cagctggtgt agtatcttta gaacctaatc
     4861 cataacgtcc accattgatt gtgcagcttt cgaattttgt tcctcttaat gcgttaacaa
     4921 catcaaggta taatggttct cttgctccag gctctttaga tctgtctaat acgttgataa
     4981 cttttgctgt atcagggatt gcttctacta atttctcagc aacgaatggt ctgtaaagac
     5041 gaactttaac aacaccgact tttttaccct gtttcattaa gtaatcgatt gtttcgtcga
     5101 ttgtttcaca aacagatccc atagcgatga ttacgctctc agcgtcttca gctccatagt
     5161 agttgaataa tttataatct gttccgatct tagcgttaac ctgattcatg taatcttcaa
     5221 cgattgcagg gaaagcatta tatgttgagt tagctgcttc tcttgcctgg aagaagatgt
     5281 ctgggttctg agctgatcca cgaagaactg gatgttctgg gtttaagcac tcagctctga
     5341 atttgtcgat tgcttcataa tcaaccatat ctgcaagatc atcataatcc cacatttcaa
     5401 ttttctgaat ttcatgagat gttctgaatc catcgaagaa atgtaataca ggaacacgtc
     5461 ctttaattgt tgctaaatga gctactgctc ctaagtccat aacttcctga acactagagc
     5521 tgcaaagcat tgcgaaacct gtctgacgac atgcataaac gtcagagtgg tcaccaaaga
     5581 tgttcagtgc gtgagatgca agtgcacgag cagatacatc aaatacgcaa ggcatctgtt
     5641 caccagcgat cttatacatg ttagggatca ttaataacag accctgagat gctgtataag
     5701 tagttgttaa tgcaccagct gctaaagaac catgtacagt tcctgctgct cctccttcag
     5761 actgcatttc aactaatttt acaggatgtc cgaaaatatt tttctttccc tgtgttgccc
     5821 actggtcagt tgactctgcc attggtgagg atggtgtaat aggataaatt gctgcagctt
     5881 ctgtaaaagc atatgatacg tgtgcagcgg ctgtgttacc atccatggtt ttcatttttc
     5941 ttgccatgtt ctcttcctcc taaaaaatct cgtgtgaata cattcacaca ttgttaatct
     6001 ctacgtccac ctagcatatg ggcgtagaaa atatcactac aactttcaca agttgcttaa
     6061 aacataaaca aagctgtata catataactt ttgtatacag ctttattcta cattatttgc
     6121 acaagttttt aaagagattt ttatgtattt ttaggtattt ttttttgtac gccaaaaaaa
     6181 taacaatctt tttgaatcct tagtaaaata ctcaagattt tgaattcgtt gtatttgtgc
     6241 tatttgtact atttttatct tttgtgcttg tgcttttcgc attcttatgt tcatcgaatg
     6301 tttttgtgaa aacatgggtt ccttttttgc ttccgtcagt tctataatat aggtaatcat
     6361 gtttcttcgg atgcatcgca gccttgatcg ccgtgtctga cggattacag attggtcctg
     6421 ctggcaaccc tttcataacg taagtattat acaccgtttt cactttcaga tctttataat
     6481 aaactttctt cgcattatat aatccatttg ttgtggtata caaaacggtc ggatcgatct
     6541 gaagtctcat tttcgctgca aggcgattct caatgactcc tgcgatcatc ggacgttctg
     6601 acatattgct tgcttcacgt tcgatcattg atgcgatcgt catgatctct gccatagaac
     6661 gctttccttt ataagacttc gcaagtgctg aatatttctt atcgaaatta tcaagcatct
     6721 tctggatcag atcttctggt ttggaacttt tgtaaatctt ataagtatcc ggatacagat
     6781 aaccttctaa tttgtacttt gttcccttct ttgggtcaat gtctttaata aaatcgtttt
     6841 tatactgatt tgtattcttc gcagccttta taaattcatc tgctttaaag actccctgtt
     6901 tctctagtcg ttttgctatc atatcaatgg aatatccttc cgggatcatg actgtaacga
     6961 ctcttcttgc ctctgcaagt tttctggcct cttctgcttt ctgctgcctg taagccatat
     7021 attttcttcc agcaaatata cctcctgcca caatgaccac tagtaaaata atgaccccaa
     7081 tgacaaacgg aactctggaa cgatttcttc ttccacgtct gcgccccctt ctgcgccttc
     7141 cggaattatg atcaaaatct aaatataaat ccatgatgtc ctcccatatt tttattatcg
     7201 catgatttta aaatcatact gtgttcctat gctattatac tcgcattttg tagaaataaa
     7261 agctttttca ttaaattttg ttcatattct taactttttt gtaatttttg tgtaaaaata
     7321 tgcatatttt ggattgtaac tcttctggtc aaactgctgt acttttttta gtatttccat
     7381 tataatcaaa gcatagccgt aaatggcaaa agattataaa atatagaaag gaatttcata
     7441 ttatggcaaa tccaattgta acgatcacta tggaaaatgg tgatgtaatg aaagcagaat
     7501 tatatccgga gatcgctccg atcactgttg agaactttgt aaatcttgta aaagacggtt
     7561 tctatgatgg tttaactttc catcgtatca tcaacggatt tatgatccag ggtggatgcc
     7621 cattaggtac aggaacaggt gggcctggac atacgatcaa aggagagttc agccagaacg
     7681 gtgtgatcaa caacttaaaa cacactcctg gtgttttatc catggctcgt tctatgatgc
     7741 ctgattctgc cggatcacag ttctttatca tgcacaaagc tgctccacat ttagatggac
     7801 agtacgcagc atttggtaag atcatcgaag gaatggatgt tgtaaataag atcgctgaag
     7861 ttgcaacaga ttattccgat aaaccattag aaccacaagt tatgaagact gtaactgtgg
     7921 aagaatagtc aactatatcc ttacagcaca gagccggacc atcaatcttc atttgtatcc
     7981 ggctcttaat ttttataaat atttagatgg ggaatgataa tatgagtgaa aacaacaaac
     8041 taatctttct ggatgtcgat gcgactttat acagtaaaga acagcgtctg gttccagaat
     8101 ctacgatcaa agcagtccat gaagcacaag aaaatggtca taaagttctg atcaacactg
     8161 gacgacctct tgtttatttt gaaaaagaga ttctcgatat tggctgcgac ggttatcttt
     8221 gttctaacgg agttcacatc cttcttgatg gcgaaacgat ctatcacaaa acagttcctg
     8281 aaacagtggt cgaacagatc aaacggattt gcgaagaaaa caatatctat ggaacctttg
     8341 aaggagaaaa atgttcttat ttccgcgatc ataac
//
LOCUS       DAOMUE010000056         8365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2201_length_8365_cov_2.390854, whole genome shotgun sequence.
ACCESSION   DAOMUE010000056 DAOMUE010000000
VERSION     DAOMUE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2201_length_8365_cov_2.390854"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /gene="cysK"
                     /locus_tag="PLC71_04030"
     CDS             <1..850
                     /gene="cysK"
                     /locus_tag="PLC71_04030"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRM01844.1"
                     /translation="KLQARLLVKLDGYNPAGSAKDRIAYQMIVDAEETGKLKEGSVII
                     EPTSGNTGIGLAAIGASKGYRVILTMPETMSVERRNLLKAYGAEIVLTDGSQGMKGAI
                     TKAEELAEEIEDAFIAGQFVNPSNPKVHRLTTGPEIWKDTDGEVDIFIAGVGTGGTVT
                     GVGEYLKSKKEDVKVIAVEPATSPMLSKGQAGPHKIQGIGAGFVPEVLNTEIYDEIIR
                     VENEDAFDYGKVIAIEEGILVGISSGAALYAAIQVAKRKENKGKTIVAFLPDNGDRYL
                     STPLFS"
     gene            851..>2114
                     /locus_tag="PLC71_04035"
     CDS             851..>2114
                     /locus_tag="PLC71_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRM01845.1"
                     /translation="MHVKCYWFSISVVLCYNVSERIYRKGEQSGVKQKLLLIDGNSIL
                     NRAFYGLPDMTNSQGLHTNAILGFLNIMFKFLEEENPTHLAVAFDLKAPTFRHKMFAD
                     YKGTRKGMPDELQEQVPVIREVLKAMNIPLMMEEGYEADDLLGTMSVLGEQAGFEVKI
                     VSGDRDLLQLATKKVQIRIPKTNRAGTVVEDYYEDDVLEKYQVTPKEFIDVKALMGDA
                     SDNIPGIPGIGEKTATKLIKEFQTIENAYAHIDEVKPNRAKNNLQEYYDQGVMSKELA
                     TIKLDSPIDISFDDAKLGDLYTKEAYHMLKELEFKAILKRFDGEEQEDLEVKIKEIID
                     LAEAEDIFAKAVKKKEAGISIYKENDAMWVALNTEGEEVYLFSCEGFGFITQDFLYEK
                     IDDLYDNIGYVAMMEVKEHLSHLTIHEMT"
     assembly_gap    2115..2124
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2125..3618
                     /gene="polA"
                     /locus_tag="PLC71_04040"
     CDS             <2125..3618
                     /gene="polA"
                     /locus_tag="PLC71_04040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRM01846.1"
                     /translation="WQGLHNIGFDEIFDVSLAAYLVNPLKSTYEYDDIARDYKSMMLP
                     SKKELIDKKHPMVTDGVLSDAGKKIMGYEAYISREAIQPLSDKLTELEMMDLYREIEI
                     PTMFALHDMEVRGIHVDSKALKEYGDQLVGRIEELQESIYKEAGEEFNINSPKQLGVV
                     LFEHMKLEGAKKTKTGYSTSVEVLEKIEHLYPIISMILEYRQLTKLKSTYADGLANFI
                     QDDQRIHGKFNQTITATGRISSTEPNLQNIPIRMELGRAIRKVFLPEDGYVFLDADYS
                     QIELRVLAHLSQDEKLIHAYEENQDIHARTASEVFGIPMDEVTSTQRRDAKAVNFGII
                     YGLSAFGLSQDLKISRKQAQEYIDRYFAMYPRVKEFLDGEVEKGKTDGYVKTMFNRIR
                     PIPELKSSNFMQRNFGERVAMNSPIQGTAADIIKIAMVRVNMKLKEKQMKSRLLLQIH
                     DELLIETHLDEFEEVKEILQNEMMNAVSLRVPLNIDIEQGASWYEAK"
     gene            3630..4217
                     /gene="coaE"
                     /locus_tag="PLC71_04045"
     CDS             3630..4217
                     /gene="coaE"
                     /locus_tag="PLC71_04045"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393738.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRM01847.1"
                     /translation="MKIIGITGGVGSGKSEILNILKNDYQAKVIQSDHVAHELMVPGA
                     KSYDAIVQAFGNEILNEDQTINRPILGEIVFHDETKLSLLNSITHKNVDEEILSRIDQ
                     FGKEEPEGLLVIESALLVGAGYEKRFDQLWYIYTREEVRYERLKASRGYSDEKIKQMI
                     EKQQKEEEFKSMASNIIDNSGDLEDTKAQIVKILG"
     gene            4325..5107
                     /locus_tag="PLC71_04050"
     CDS             4325..5107
                     /locus_tag="PLC71_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM01848.1"
                     /translation="MKLSSLASSSEGNCIYVGTKKTNVLVDCGVSAKRIENSLSELDL
                     TVPEFDGILITHEHTDHIKGLGVIARRYGLPIFATGKTIDAIFDYKNLGKVDKSLFHS
                     IEADKPFEIGDMKVNASHIWHDAADPVCYSFYSEEGAKASIATDLGDYDEYLVEKIYD
                     SDILFVEANHDVNMLQVGRYPYYLKRRILGREGHLSNERCAELIEEVATQKTKKVYLG
                     HLSKENNYEKLAYETVKISLHNFTLPIEVARRDTVSTVTSVS"
     gene            5130..5402
                     /locus_tag="PLC71_04055"
     CDS             5130..5402
                     /locus_tag="PLC71_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRM01849.1"
                     /translation="MEKTVITVVGKDGVGIIAKVCNYLASNNVNILDISQTITGGFFN
                     MMMVVDPAGSSKDIALLSSELKQIGEEIGVVIQAQNERIFDIMHRI"
     gene            5415..6779
                     /locus_tag="PLC71_04060"
     CDS             5415..6779
                     /locus_tag="PLC71_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HRM01850.1"
                     /translation="MINLNEVFETNKMIHEMNLDVRTITIGISLLDCVGTTLEEVKEN
                     IYNKITTVAKDLVKTGKDIENEFGIPIVNKRISITPISLVGGNVCKTPDDFVELAKVL
                     DAAAKKVGVNFLGGYSALVSKGMTKADELLIRSIPKALAETDFVCSSVNVGSTKTGIN
                     MDAVKLIGEIIKETAELTKDNQCLGCAKFVVFCNAPDDNPFMAGAFHGVTEADAIINV
                     GVSGPGVVKRAIENVRGENFEVLCETIKKTAFKVTRVGQLVAKEASKRLGIPFGIIDL
                     SLAPTPAAGDSVGEILEEIGLEYAGAPGTTAALAMLNDQVKKGGVMASSYVGGLSGAF
                     IPVSEDQRMIDAVNAGALTIEKLEAMTCVCSVGLDMIAIPGKTKATTIAGLIADEMAL
                     GMINQKTTAVRVIPAIGKDVGDQVEFGGLLGYAPIMPVNEFSCDAFVNRGGRIPAPIH
                     SFKN"
     gene            7030..8259
                     /gene="tyrS"
                     /locus_tag="PLC71_04065"
     CDS             7030..8259
                     /gene="tyrS"
                     /locus_tag="PLC71_04065"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRM01851.1"
                     /translation="MSQNVYDVLQERGFIAQTTHEELRELLGKEKVTFYIGFDATADS
                     LTAGHFLTIMAMMHMQKAGHRPIALLGGGTTMIGDPSGKSDMRNMLTKETIDHNAKRF
                     KEQLSRFITFDDDKAILANNADWLLNLNYVEFLREVGVHFSVNKMLTAECYKQRMERG
                     LTFFEFNYMLMQGYDFLELNRRYGCKLEMGGNDQWSNILAGADLIRRKEKKDAYGLTL
                     TLLTRSDGVKMGKTMAGAVWLDPEKTSPYDFYQYWRNIEDVKVEECLSLLTFLPMDEV
                     RRLGALEGAEINKAKEVLAYEVTKIVHGEEEAKKAQTAAKAMFVSGGANEDAPTFEYE
                     ADKLAEGIDILTMLVDSKLCTTRSDARRMVQQGGVSVNGKKVDAIDATFGKADFDDKD
                     RILLKKGKKKFCQIKEK"
BASE COUNT         3014 a         1205 c         1928 g         2208 t
ORIGIN      
        1 taaactacag gcaagattat tagtaaaact agatggatat aatccagcag gaagcgcaaa
       61 agaccgtatc gcatatcaga tgatcgttga tgcagaagaa acaggaaaat taaaagaagg
      121 gtctgttatt atagaaccaa catcaggaaa tacagggata ggacttgcag cgatcggtgc
      181 atccaaagga tacagggtaa tcctcacaat gccagagaca atgagtgttg aacgaagaaa
      241 tctattaaaa gcttatggag cggaaattgt attaacagat ggttcacaag gtatgaaagg
      301 agcaatcaca aaagctgaag aacttgctga agaaatcgaa gatgcattta tcgcaggaca
      361 gtttgttaat ccatccaatc cgaaagttca cagactgaca acagggccag agatttggaa
      421 agacacggac ggagaagtag atatttttat tgcaggagtt ggaacaggag gaactgttac
      481 aggagttgga gaatatttaa aatccaagaa agaagatgtg aaagtgatcg ctgttgaacc
      541 agcaacatct ccaatgcttt caaaaggtca ggcaggacca cataagattc aaggaatcgg
      601 agccggattt gtgcctgaag tgttaaatac agaaatttat gatgagatca ttcgagtgga
      661 gaatgaggac gcttttgatt atggaaaagt gatcgcaata gaagaaggaa tcttagttgg
      721 aatttcatca ggtgctgcat tatatgcggc gattcaggtg gcaaaacgta aggaaaataa
      781 agggaagaca attgtagcat ttttgccaga taatggagat cgatatttgt caacaccatt
      841 gttttcataa atgcatgtta aatgttattg gttttcaatt tctgtagttt tgtgttacaa
      901 tgtctcagaa cgcatttata gaaaaggaga acaatctgga gtgaaacaga aattattatt
      961 gattgatgga aacagcattt taaaccgtgc attttatgga ttgcccgaca tgaccaatag
     1021 tcaaggactc catacgaatg cgatccttgg ttttttgaat attatgttta aatttttaga
     1081 ggaagagaat ccgacacatc tggcagttgc atttgactta aaagcgccaa cattccgcca
     1141 taaaatgttt gcggattata aaggaacgag aaaaggaatg ccagatgaat tacaagaaca
     1201 ggttccagtg atccgtgaag tgttaaaggc aatgaacatt cctttgatga tggaagaagg
     1261 ttatgaagca gatgaccttc ttgggacaat gtctgttctt ggagaacaag caggatttga
     1321 agtgaaaatt gtctctggag atcgagatct tttacagctt gcgacaaaga aagtgcagat
     1381 tcgtattcca aagacaaaca gggctggaac ggttgttgaa gattattatg aagatgatgt
     1441 gttagaaaaa tatcaggtaa cgccaaagga atttattgac gtgaaagctt tgatgggtga
     1501 tgcatcggat aatattcctg gaattcctgg aataggggag aagacagcga caaaactgat
     1561 caaagaattt caaacaattg aaaatgcata tgcacatatt gatgaagtaa aacccaatcg
     1621 tgcaaaaaac aatctgcagg aatactatga tcagggtgtt atgagtaaag aacttgcaac
     1681 gatcaagtta gacagtccga tcgatatttc ctttgatgat gcgaagcttg gtgatctgta
     1741 tacgaaagaa gcgtatcata tgttgaaaga attggaattt aaagcaattc tcaaacgttt
     1801 tgatggagaa gaacaagagg atctcgaagt taagatcaaa gagatcatcg atcttgcaga
     1861 ggcggaagat atttttgcaa aagcagtgaa gaagaaagaa gcaggaatct ccatttataa
     1921 agaaaatgat gcaatgtggg ttgcattaaa tacagaagga gaagaagtat atttatttag
     1981 ttgtgaaggt tttggattta taacgcaaga ttttttatat gagaaaattg atgatctata
     2041 tgacaacata ggatatgtgg caatgatgga agtgaaagaa catttgtctc atttaacgat
     2101 ccatgagatg acaannnnnn nnnntggcag ggcctgcata atataggctt tgatgaaatt
     2161 tttgatgttt cactggctgc ttatcttgtg aatccattaa aaagcactta tgaatatgat
     2221 gatattgcaa gagattataa atctatgatg cttccatcca aaaaagaatt gatcgataag
     2281 aaacatccaa tggttacaga tggggtttta agtgatgcag gaaagaagat catgggttat
     2341 gaagcttata tttcaagaga agcgatccaa ccattaagtg ataaattaac ggaacttgag
     2401 atgatggatc tttatcgaga gattgagatt ccaacaatgt ttgcacttca tgatatggaa
     2461 gtacgaggaa ttcacgtaga ttcgaaagcg ttgaaagaat atggagatca gctggttgga
     2521 aggattgaag aattacagga gtctatctac aaagaagcag gggaagagtt caatatcaat
     2581 tctccaaaac agttaggtgt tgttttattt gaacatatga aattagaagg tgcaaaaaag
     2641 acaaagactg gatattccac cagtgtggaa gtgttagaaa agatcgagca tctctatccg
     2701 atcatttcga tgatcttaga atacagacag ttgacgaaat taaaatcaac gtatgcagat
     2761 ggattagcaa attttattca ggatgatcag agaattcatg ggaaattcaa tcagacgatc
     2821 acagcgacag ggagaatcag cagtacggaa ccaaatcttc agaatattcc aatccgtatg
     2881 gaacttggaa gagcaatccg taaagtattt ctgccagaag atggatatgt attcttagat
     2941 gcggattatt cacagatcga acttcgagtt ctggcacatt tgtcacagga tgagaaactg
     3001 atccatgcat atgaggagaa tcaggacatt catgcaagaa cagcttctga ggtatttgga
     3061 attccgatgg atgaagtgac gagtacacaa agaagagatg caaaagcggt taactttggg
     3121 atcatttatg gattaagtgc gtttggttta agtcaggatc ttaagatttc aagaaaacag
     3181 gcacaggaat atattgatcg ttattttgca atgtatccac gagttaagga attcttagat
     3241 ggggaagtgg aaaaaggaaa aacagacgga tatgtaaaga caatgttcaa tcgaatccgt
     3301 ccgatcccag aattaaaatc cagcaatttc atgcagagaa acttcggaga acgtgtagca
     3361 atgaattccc cgattcaggg aacagcagca gatatcatta agattgcaat ggtaagagtg
     3421 aacatgaaat taaaagaaaa acagatgaaa tcccgtctgc ttttacaaat tcacgatgaa
     3481 cttctgattg aaacacattt ggatgaattt gaggaagtca aagaaatctt acagaatgaa
     3541 atgatgaatg cagtaagcct tcgtgtgcca ttaaatattg atattgaaca gggagcaagc
     3601 tggtacgaag caaaataagg agaagataaa tgaaaatcat aggaattacc gggggagtcg
     3661 gctctggaaa aagtgagatt ttaaatatac taaaaaatga ttatcaggca aaagttattc
     3721 aatctgatca tgtagcacat gagttgatgg taccaggggc aaaaagttat gatgcgatcg
     3781 ttcaggcatt tggaaatgaa attttaaatg aagaccagac gatcaatcgt ccgatacttg
     3841 gagagatcgt gtttcatgat gaaacaaaac tttcattatt aaacagcatc actcataaaa
     3901 atgtagatga agagatttta tcaagaattg atcagtttgg aaaagaagaa ccagaaggtt
     3961 tgcttgtcat agaatcagct ttgttagttg gagcaggata tgaaaagaga tttgatcagt
     4021 tatggtatat ttacacgaga gaagaagtaa gatatgaacg cttaaaagct tccagaggat
     4081 attctgatga aaagatcaaa cagatgatcg aaaaacagca aaaagaagaa gagtttaaat
     4141 ctatggcaag caatatcatt gataattcag gtgatctaga agatacaaaa gcacagatcg
     4201 taaagattct tggttgatat tttgtacatt taagaataca gagcaattct tgaatcttaa
     4261 ataaccttat gatataatgt ttgccaatag ggtcagccca gaagagaaga aagaggtaca
     4321 atccgtgaaa ctatcaagtt tagcaagcag cagtgaagga aattgcatat atgttggaac
     4381 gaagaagacg aatgtattag ttgattgtgg agtcagtgca aagcgtattg aaaactccct
     4441 ttcagaactt gaccttaccg ttccagaatt tgatggaatt ttgatcacac atgagcatac
     4501 ggatcatatc aagggacttg gtgttatcgc aagacgatat ggacttccaa tctttgcaac
     4561 aggaaagacg atcgatgcga tttttgatta taagaacctt gggaaagttg ataaaagcct
     4621 ttttcattcg attgaagcag acaaaccatt tgagatcgga gatatgaaag tcaatgcatc
     4681 tcatatatgg catgatgctg cagatcctgt ttgttactcg ttttactcag aagaaggggc
     4741 caaggcaagc attgcaacag atcttggaga ctacgatgaa tatctggtag agaagatata
     4801 tgattcggat attttatttg tagaagctaa tcatgatgtg aatatgcttc aggttggacg
     4861 ctatccgtat tatttgaaga gacggatcct tggaagagag ggacatttat cgaatgaaag
     4921 atgtgctgaa ctgattgagg aagttgcaac acagaagaca aagaaagttt atcttggaca
     4981 tctgagtaaa gagaataatt acgaaaaact ggcatacgag acggtaaaga tttctttaca
     5041 taattttaca ttaccgatcg aagtagccag aagagacacg gtatctaccg taaccagtgt
     5101 atcataaaca tttaggagga ataacaaaaa tggaaaaaac agtaatcaca gttgttggaa
     5161 aagacggtgt aggaatcatc gcaaaggtgt gtaactattt agctagcaac aatgttaaca
     5221 tcttagacat ctcccaaacg atcacaggcg gcttctttaa catgatgatg gtcgtagatc
     5281 cagctggttc atccaaagat atcgcattat tatccagtga gttaaaacag atcggtgaag
     5341 aaatcggtgt tgtaattcag gcgcagaacg aaagaatctt tgatattatg cacagaattt
     5401 agtggggtgg catcatgatt aatctgaatg aagtatttga aacgaataaa atgatccatg
     5461 agatgaattt agatgttcgt acgatcacga tcggtatcag tttgttagat tgtgttggta
     5521 caacactaga agaggtcaag gagaatattt ataataagat cacaacagtt gcaaaagatc
     5581 ttgtgaagac aggaaaagat atcgagaatg aatttggtat tccaattgtt aacaaacgaa
     5641 tttctatcac accaatttct ttagtgggtg ggaatgtatg taagacacca gatgatttcg
     5701 tagaacttgc gaaagtatta gatgcagcag caaagaaagt tggagtcaac ttcttaggtg
     5761 gatattctgc attagtaagc aaaggaatga caaaagcaga tgaattattg atccgttcta
     5821 ttccaaaagc attagcagag acagactttg tatgttcctc tgttaatgta ggttctacaa
     5881 agacaggaat caacatggat gcagtgaaat tgatcggtga gattatcaaa gaaacagcag
     5941 aacttacaaa agacaaccag tgtcttggat gtgcgaagtt cgtagtattt tgtaatgcac
     6001 cagatgataa cccatttatg gcaggagctt tccatggagt tacagaagca gatgcgatca
     6061 tcaatgtagg tgttagtgga ccaggtgttg taaaacgtgc gattgaaaat gttagaggag
     6121 agaactttga agttctttgc gagacgatta agaaaacagc gtttaaagta actcgtgtcg
     6181 gacagttagt agcgaaagaa gcatctaaga gacttggaat tccatttggg atcattgatc
     6241 tttctcttgc accaacacca gcagcaggag acagtgttgg agagatctta gaagagattg
     6301 gattagagta tgcaggagca ccaggaacaa cagcagcctt agcgatgtta aatgaccaag
     6361 tgaagaaggg tggagttatg gcttcctctt acgttggtgg attaagtgga gcatttattc
     6421 cagtcagcga agatcagaga atgatcgatg cagtgaatgc aggagcttta acaattgaaa
     6481 aattagaagc aatgacatgt gtatgttctg taggacttga tatgatcgca atcccaggaa
     6541 aaacaaaagc aacaacgatc gcaggactga tcgcagatga gatggcactt ggtatgatca
     6601 atcagaagac aactgctgtc cgtgtgatcc cagcaatcgg aaaagatgtt ggagatcagg
     6661 tagaatttgg tggattactt ggatatgcac caattatgcc agtgaatgaa ttttcatgcg
     6721 atgcatttgt gaatcgtgga ggaagaattc cagcaccgat ccatagcttt aaaaactaga
     6781 aatcaagata tttttgaggt gacaattttg gggtattaga tcagcatgca gacattatat
     6841 catcagaatc agatatggtg gacatgcata caggaagaga acgaatgtcc ggatcatctg
     6901 ttaagagaac ttgggaacgg ctgcataaga ttatagagga attcccataa gttcaaagga
     6961 acggcatagg ccgtggacta tattatataa acacaaatta agaagagaat ttaaaaggag
     7021 atatataaaa tgagtcaaaa cgtatatgat gtgctgcaag aaagaggatt tatcgcacag
     7081 acaacacacg aggaattaag agaactgtta ggaaaagaaa aagtaacatt ctatatcgga
     7141 tttgatgcaa cagcagacag tttaacagca ggacattttt taacgatcat ggcaatgatg
     7201 catatgcaga aagcaggaca tcgtccaatt gctcttttag gtggcgggac aactatgatt
     7261 ggagatccat ctggaaaatc tgatatgaga aatatgttaa caaaggaaac gatcgatcat
     7321 aatgcgaaac gtttcaaaga acagttgtca agatttatca catttgatga tgacaaagca
     7381 atcttagcaa ataacgcaga ttggttatta aatttaaatt acgttgaatt cttacgtgaa
     7441 gttggagttc atttctcagt caacaagatg ttaacagcag aatgttacaa acagagaatg
     7501 gaacgtggac ttacattctt cgagtttaac tatatgttaa tgcagggata tgatttcctt
     7561 gagttaaata gaagatatgg atgtaaatta gagatgggtg gaaacgacca gtggtctaat
     7621 atccttgcag gagctgactt gatccgcaga aaagaaaaga aagatgctta cggattaaca
     7681 ttaacacttc ttacaagaag cgacggagtg aagatgggta aaacaatggc aggtgctgta
     7741 tggcttgatc cagagaagac atctccatac gatttctacc agtactggag aaatattgaa
     7801 gatgtgaaag tagaagaatg cttatcactt cttacattcc ttccaatgga tgaagtccga
     7861 agactgggag ccttagaagg agcagagatc aacaaagcaa aagaagttct tgcttatgaa
     7921 gttacaaaga tcgttcatgg agaagaagaa gcgaagaaag ctcagacagc tgcaaaagca
     7981 atgtttgtta gcggtggagc aaacgaagat gcccctacat ttgaatatga agcagataaa
     8041 ttagcagaag gaatcgatat tcttacaatg ttagtagatt ctaaactttg cacaactcgt
     8101 tcagatgcaa gaagaatggt acagcagggt ggagtttctg taaatggtaa gaaagtagat
     8161 gcaattgatg caacatttgg taaagctgat tttgatgata aagatcgtat cttattaaag
     8221 aaaggtaaga agaaattctg ccagatcaaa gaaaaataat taaaaaataa acaagtagga
     8281 atcccggaga gttgatactc cggggttctt ttcaatattc aaaaggaaaa taaaaaagat
     8341 atattagaat taatgccaca tgttg
//
LOCUS       DAOMUE010000057         8338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2213_length_8338_cov_2.475432, whole genome shotgun sequence.
ACCESSION   DAOMUE010000057 DAOMUE010000000
VERSION     DAOMUE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2213_length_8338_cov_2.475432"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLC71_04070"
     CDS             <1..580
                     /locus_tag="PLC71_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM01852.1"
                     /translation="MPFITEEIYCNLTDEESIMLAKWPEFKEEWNFKADEKAVETIKE
                     AVRGIRNVRAEMNVSPKKKAQVYVVSEDAEIRDIFEQGKVFFATLGYASEVIIQDNKD
                     GIGDDAVSSVIAGAVIYMPFAELVDIAKEKERLAKEEKRLNGEIKRCEGMLGNERFVS
                     KAPEAKVQEEKDKLAKYQQMLEQVKEQIARIS"
     gene            617..1360
                     /locus_tag="PLC71_04075"
     CDS             617..1360
                     /locus_tag="PLC71_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HRM01853.1"
                     /translation="MEFTLDLHTHTVASGHAYSTVQEMAKAAADKGLKLLGITEHAQG
                     IPGTCDEIYFHNMRIIPRKMYGIDLMFGSEINIIDHDGTLSMEEKIIEKTLDIRIAGI
                     HLPCYEVGTVTQNTNAYVKAIENPMIDIISHPDDSRIPIDYETIVDAAKENHTLLEIN
                     NSSLDSLSSRVGAWQNLQIMLQMCEEKKVPVVAGTDAHFSSAVGVFDHVEVLLQEMNF
                     PEELVVNRSVDVLKQEILQHREIRKIGRD"
     gene            complement(1411..2343)
                     /locus_tag="PLC71_04080"
     CDS             complement(1411..2343)
                     /locus_tag="PLC71_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRM01854.1"
                     /translation="MEHNIQQSLFFDIETTGLSADISAITVIGCCDMDGNVTQWFNED
                     GLSQKQILTDFLAFIQPYNTLITFNGKTFDLPFLTSKIKEFKINASFDQYEHLDLYQI
                     LKPYKNLWGLKNFRQKNLEEYLGIQRIDKLSGKKLIKTYQDYLENGEIKNKESLLLHN
                     HEDLIGLHKIYSLMSYPALLDKEFTLSSYFIENDHFVAHLNLDIQIPVSCNYEKSGIS
                     LTLDHSNAILTCPLENGHLKHYLKDYKNYYYLPMEDLVIHKSMKSFVDTTSIIRADKN
                     NCYSKFTPGDSFLSAKTDVTNYCADIIYYILSEK"
     gene            2574..3179
                     /gene="ruvA"
                     /locus_tag="PLC71_04085"
     CDS             2574..3179
                     /gene="ruvA"
                     /locus_tag="PLC71_04085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRM01855.1"
                     /translation="MISYIKGILEDMSPGMVVVDNHGIGYQMMVPMRGESFPKIGQEI
                     KIYTHMHVREDDVSLFGFLSKEEKEAFELLIGVNGIGPKVGLSVLSTLSVYELKMAVI
                     SEDVKTISKTPGLGPKGAKKLILELKDKLSFEELEEDGVGAEIFDTSADSSDSVMITI
                     EGLVSLGYSKSEAAIAVNKVEDAKDLTPEELLKKALKNIMI"
     gene            3193..4197
                     /gene="ruvB"
                     /locus_tag="PLC71_04090"
     CDS             3193..4197
                     /gene="ruvB"
                     /locus_tag="PLC71_04090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRM01856.1"
                     /translation="MQNRMISTELVEEDIKIENHLRPQMLSDYIGQEKVKENLKIFIE
                     AAKSRGESLDHVLFYGPPGLGKTTLAGIVANEMGVHLKVTSGPAIEKPGEIAAILNNL
                     QEGDVLFIDEIHRLNRQVEEVLYPAMEDYAIDILVGKGQAARSIRLELPRFTLVGATT
                     RAGMLTAPLRDRFGVVNRLNFYNSEELQTIIERSAEVLEIGIDPEGALEIARRSRGTP
                     RLANRLLKRVRDFAQVKYDGQITLEIARDALNHLEVDTIGLDQTDRNLLKTMMETFEG
                     RPVGLDTLAAAIGEDSGTIEDVYEPYLIQNGLIQRTPRGRVVTRSAYEHFHLPIPEDR
                     "
     gene            4201..6582
                     /locus_tag="PLC71_04095"
     CDS             4201..6582
                     /locus_tag="PLC71_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRM01857.1"
                     /translation="MRTKNRPIEVLAPAGSMECLKAAILNGADAVYLGGEMFGARAYA
                     GNFNKEELLEAIDYVHLYGKKLFLTVNTLMKEEELPLLPGFLKPFYEQGLDAVIVQDL
                     GAVSVIRKHFPKLEIHASTQMTITGVHGARIAKKMGAKRVVPARELSLEEIQEIKDDT
                     GLDMECFVHGALCYCYSGQCFMSSMLGGRSGNRGRCAGTCRLPFYLDGTKNTKNAYPL
                     SLKDLCTIEHLPEILDHGVDSLKIEGRMKGPRYVGEVTRIYRKYVDLYLSEKPYKVES
                     EDLKILMEVFNRGGFTGGYYKEYHGKDMMSMKRPDHQGLYVGKISKLMKGKISFTAQE
                     DIHKGDALQIRINSEEKVELTSPSEFKAGSKVVLNGQKMKKLHEGMEIKRTLNHPMIE
                     RIDEGLKQKKKENLKGKIIIQKDQCAKLILKDGEDHVEVIGPVIEEAQKNGAMPADIE
                     KLLKKTGQTHYTFEELEVDLGENLFVPGSVLKKLRKEAFAQMDQTKILKYRRTYEDPV
                     KENDVASSQEQEDSKIQIAVSMENLEYLDIVTEDREVMKIYLSWMELKQQEDPQSILN
                     KVKTAGKQCYLMLPQIARKRQMEELKAKKDLIFTNEIDGLLVRNLEEFSWLIEEGYQK
                     EMIADYMFYGYNKEAVKEYQRLSGIQIRMTYPSELNKKEMEQLELLNADLFLYGYQPL
                     MVSAQCVKNNLRGCDKTPSWLTLKDRYNANFFVHTSCCDCINEIYNGKPLWVGNEAGI
                     LNDLHPSVIRFHITRENEVQVKEILNAAKSIQKGERASLATEYTTGHLKREVL"
     gene            6589..7965
                     /locus_tag="PLC71_04100"
     CDS             6589..7965
                     /locus_tag="PLC71_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRM01858.1"
                     /translation="MIDIITTIAKYLIIIMCLIYTLSCYTVFRPKNRDRKEDLLDNQV
                     IYMFVFLFLCYTVLFLQEFELKILIFFAAQVIFFEILMIVFPRIYKRCSRPLINNTCF
                     LLGVGFVILTRLSFDLAMKQFAIAAGSVIVTSFIPLMMHKITIWNKFGWLYAVAGFLL
                     LSTVFVFGINKYGAYNWVSIAGLKFQPSEFVKIIFVFFVAALLSKAKEFKDLVKITVI
                     AALYVLVLVVEKDLGGALLYFVIYLMMLYVATAKASYLFGGLAAGSVAAIIADKIFTH
                     VQIRVAVWKDPFSMIEGRGLQVCQSLFAIGTGSWFGMGLGNGRPFDIPVRESDFVFSV
                     ICEEFGVIFGICLIFVLMSSFILFMDISTRSRKLFNKLLCLGFGVCFLFQVFLSIGGV
                     TKFIPSTGVTIPLVSYGGTSVISTLIIFNIIQGLHMLADSEEEEYEHIKAQESEKQYT
                     RQNNRKNQ"
     gene            7898..>8338
                     /locus_tag="PLC71_04105"
     CDS             7898..>8338
                     /locus_tag="PLC71_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRM01859.1"
                     /translation="MSILRHKNQKNNIQDKTTERISKIKKKKKKRANRQILQITYIFV
                     VSFLALIAYIAHFVAVDSKNVITNAHNRRLQSMAEKVVRGKIISSDGKVLAQTLTENG
                     SEIRDYPYGRMFAHVVGYNDKGKSGLESVCNFDLLKSDANLMTQI"
BASE COUNT         2882 a         1259 c         1883 g         2314 t
ORIGIN      
        1 tatgccattt atcacagaag agatttattg taatttaaca gatgaagaat ctatcatgtt
       61 agcaaaatgg ccagagttca aagaagaatg gaacttcaag gctgatgaga aggctgtaga
      121 gacgattaaa gaagcagtca gaggaatccg taatgttcgt gcagagatga acgtatcacc
      181 aaagaagaaa gcacaggttt acgtggtatc tgaagatgcg gagatcagag atatttttga
      241 acaagggaaa gttttctttg ctacacttgg atatgcaagt gaagttatca ttcaagacaa
      301 caaagacgga atcggagacg atgcagtatc aagtgtgatc gcaggagctg tgatctacat
      361 gccatttgca gaattagttg acattgcaaa agagaaagaa cgtcttgcaa aagaagaaaa
      421 acgtttaaat ggcgagatca aacgttgcga aggaatgctt ggaaatgaaa gattcgtaag
      481 caaagcgcct gaagcgaaag ttcaggaaga aaaagacaaa cttgccaaat atcagcagat
      541 gttagaacag gtcaaagaac agatcgcaag aatttcataa agatcagaag tggatgataa
      601 aacgaggttt tgaaaaatgg aatttacatt agatttacac acacatacag tagccagtgg
      661 acatgcgtat tcaacagtac aggagatggc aaaagcagcg gcagataaag gattgaaact
      721 tctaggcatc acagaacatg cacaaggaat tcctggaaca tgcgatgaga tttatttcca
      781 taatatgcgt attattccaa gaaagatgta tgggatcgat ctgatgtttg gctctgagat
      841 caacattatc gatcatgatg gaactttaag tatggaagag aaaattattg agaagacatt
      901 ggatatccga attgcgggaa tccatcttcc atgttatgaa gttggaaccg tcacgcaaaa
      961 tacgaatgcc tatgtaaaag cgattgagaa tccaatgatc gatattatca gtcatccaga
     1021 tgacagcaga ataccgattg attatgaaac gatcgtagat gcggcaaaag aaaatcatac
     1081 actgctagag ataaataaca gttccttgga ttctttgtca tcaagagttg gagcatggca
     1141 gaatctgcag atcatgcttc agatgtgtga agagaagaaa gtgccggtag tcgcagggac
     1201 agatgcgcat ttttcaagcg cggttggcgt gtttgatcat gtggaagtct tattacaaga
     1261 gatgaatttc ccagaagaac tagtagtgaa tcgctctgta gatgtgttaa aacaagaaat
     1321 tttacaacat cgtgaaatcc gaaagatcgg aagagattaa aaatagaaac atccatcgag
     1381 aattaaagtt cagatggatg tttttttact ttatttttca gataaaatgt aataaataat
     1441 gtcagcacaa taattggtaa catcagtttt tgcagataag aagctatctc ctggagtaaa
     1501 ttttgaataa caattgtttt tatcggcacg aatgatactc gtagtatcca caaatgactt
     1561 catactttta tggatcacaa gatcttccat agggaggtaa taataatttt tataatcttt
     1621 gaggtaatgt tttagatgac cattttccaa aggacatgta agtatagcat ttgaatgatc
     1681 aagagtcaga gatatgccag acttttcgta attgcagctg acaggaatct gtatatcaag
     1741 attcagatga gcaacaaaat gatcattttc aataaaatag gatgataaag taaattcttt
     1801 atcaagcaat gcgggataag acatcaatga ataaatttta tgtagaccga taagatcttc
     1861 gtggttatgt aaaagtagcg attctttatt cttgatctct ccattctcaa gataatcctg
     1921 ataagttttg atgagttttt ttccagaaag tttgtcgatt cgttgaattc ctaaatattc
     1981 ttccagattc ttctgccgga aattctttaa tccccacaga tttttataag gttttaaaat
     2041 ttgataaaga tcaagatgtt catactgatc aaatgatgca ttgatcttaa actccttgat
     2101 cttcgatgtc aaaaaaggaa gatcaaaggt tttgccatta aatgtaatca gtgtattata
     2161 aggttggata aatgcaagaa aatcagttaa gatctgtttc tgagataatc cgtcttcatt
     2221 aaaccattgt gttacatttc catccatatc acagcatccg atcacagtga tcgctgagat
     2281 atcagcactc aatccagttg tttctatatc aaaaaataaa gattgttgta tgttatgttc
     2341 cataaaaatc ctttctgtga acaagaatca tatccacaga tttattatat cggcttttga
     2401 tcggatttgc aatgaatgga aaaccagagt atgacactcc atcatatgat catttaagat
     2461 aagaacatat gtacggatta tttgtgaatt ctgatccaat agactggact gttaagacat
     2521 gataaggtat aatacgaaac gaatggaaaa agacagaata aggagaaacc aaattgattt
     2581 catatataaa aggaatacta gaagatatgt caccagggat ggtggttgtg gacaatcatg
     2641 ggattgggta ccagatgatg gttccgatga gaggagaatc ttttcctaag atcggacaag
     2701 aaatcaagat ctatacccat atgcatgtaa gagaagatga tgtcagttta tttggctttt
     2761 tatcaaaaga agaaaaagaa gcatttgaac ttctgatcgg agtcaatggg atcggaccga
     2821 aggtaggatt gtctgtatta tcaacgctgt ccgtttatga acttaagatg gcagtgatca
     2881 gtgaagacgt caagacaatt tctaagaccc cgggacttgg acctaaggga gcgaagaaat
     2941 tgatcttaga attaaaagat aagttaagct ttgaagaatt agaagaagat ggtgtgggag
     3001 cagagatctt tgatacatct gcagacagca gtgattctgt gatgatcacg atcgaaggat
     3061 tagtaagtct tggatattca aaaagtgaag cagcgatcgc agttaacaag gtagaagatg
     3121 cgaaagatct caccccagaa gaacttctta aaaaagcatt gaagaatatt atgatataga
     3181 tagagggtaa ttatgcaaaa cagaatgatt tccacagaac ttgtggaaga agatatcaaa
     3241 attgaaaatc atctgcgccc acaaatgtta agtgattaca ttggacagga aaaagtcaaa
     3301 gagaatttaa agatttttat tgaggcagcc aaaagtcgag gagaatcttt agatcatgta
     3361 ctgttttacg gccccccggg attaggaaag acaacacttg cagggatcgt agcaaatgaa
     3421 atgggtgtac atttgaaagt aacaagtgga ccagcgatcg agaaaccagg agaaatcgcg
     3481 gcaattctta ataatctgca ggaaggagat gtcttattta ttgatgagat tcaccgttta
     3541 aatcgacagg tagaagaagt tctgtatcca gcaatggaag actatgccat tgatatatta
     3601 gtaggaaaag gacaggctgc gagatcgatc cggctggaac tgccaagatt tacattagtc
     3661 ggggcaacga caagagctgg aatgttaaca gcaccgttaa gagatcgatt tggagttgta
     3721 aaccgcttga atttctataa cagtgaagag ttacagacga tcattgaacg gtctgcagaa
     3781 gtcttagaga ttgggatcga tccagagggt gcattagaga ttgcaagaag atccagaggg
     3841 acaccaagac ttgccaatcg tttattgaaa cgtgtgcgag attttgcaca ggtaaaatac
     3901 gatggacaga tcacattaga gattgcaaga gatgcactga atcatttaga agttgatacg
     3961 atcgggctgg atcagacaga tagaaattta ttaaagacga tgatggagac atttgaagga
     4021 agaccagtcg gacttgatac attagcagca gcgatcggag aagattcagg aacgattgaa
     4081 gatgtgtatg agccatatct gatccagaac ggattgattc agagaacacc aagaggaaga
     4141 gttgtaacaa gaagtgcata tgagcatttt catttaccaa taccagagga tagataacag
     4201 atgagaacaa agaacagacc aattgaagta ttagctccag ctggttccat ggaatgccta
     4261 aaagcagcaa ttctaaatgg agcggatgcc gtctatttag gtggagagat gtttggagcc
     4321 agagcttatg ccgggaattt taataaggaa gaactattag aagcaatcga ttacgtacat
     4381 ttgtatggta aaaaattatt tcttacagtc aatactctga tgaaagaaga agaacttcca
     4441 ttactgccgg gatttttaaa acctttttat gagcaaggac tggatgcggt gattgtacag
     4501 gatttgggag ctgtatccgt gatccgaaaa cactttccga aacttgagat tcatgcgagc
     4561 acccagatga cgatcacagg ggttcatgga gcaaggatcg caaagaaaat gggtgcaaaa
     4621 cgagttgttc cagcaaggga attatctttg gaagagattc aggagattaa ggatgataca
     4681 ggacttgaca tggaatgttt tgttcacgga gcattatgct actgctattc aggacagtgt
     4741 tttatgagta gtatgttagg tggaagaagt ggaaatcgtg ggcgatgtgc aggaacatgc
     4801 cgtctgccat tttatcttga cgggacaaag aatacaaaga atgcgtatcc attaagtttg
     4861 aaagatttat gtaccattga acatttacca gagattttag atcatggagt ggattcctta
     4921 aagatcgaag gacgcatgaa aggtccaaga tatgtcgggg aagtcacaag aatctaccgc
     4981 aaatacgtag atctttatct ttcagaaaaa ccatataaag tagaatccga ggatctgaag
     5041 atcttgatgg aagtatttaa ccgtggggga tttactggcg gatattataa agaatatcat
     5101 ggaaaagata tgatgagtat gaaacggcca gatcatcagg ggttatatgt tgggaagatc
     5161 agcaaactta tgaaaggtaa gatttctttt acggcccaag aagatatcca caaaggagat
     5221 gctttgcaga tccgtatcaa ttcagaagaa aaagtagaac ttaccagtcc ctctgaattt
     5281 aaagcaggca gcaaggttgt tttaaatgga cagaaaatga agaaacttca tgaaggcatg
     5341 gaaatcaaaa gaaccttgaa tcatccaatg atcgaacgga ttgatgaagg attaaaacag
     5401 aaaaagaaag aaaatcttaa gggaaaaatc ataattcaga aagatcagtg tgctaaactc
     5461 attctcaaag acggagaaga tcacgtagaa gtaattggtc cagtgatcga agaagcgcag
     5521 aaaaatggtg ccatgccagc agatattgag aagcttctta agaaaacagg acagacccac
     5581 tatacatttg aagaattaga agttgatctt ggagagaact tgttcgtacc aggttctgtg
     5641 ctcaagaaac taagaaaaga agcatttgca cagatggatc agacaaagat tttaaaatac
     5701 agaagaactt acgaagatcc agtcaaagaa aatgatgtag caagcagtca ggaacaggaa
     5761 gacagcaaga tacagattgc agtttctatg gagaaccttg agtatctgga tattgtaaca
     5821 gaagacaggg aagtaatgaa gatctatctt tcatggatgg aactcaaaca gcaggaggat
     5881 ccacaatcta tcctgaataa agtaaagact gcaggaaaac agtgttatct gatgcttcca
     5941 cagattgcaa gaaaacgtca gatggaagaa ttaaaggcaa agaaagatct tatatttaca
     6001 aatgaaattg atgggctgct cgtacgcaat ttagaagaat tttcatggtt gatcgaagaa
     6061 ggataccaaa aggaaatgat cgcggattac atgttttatg gatataacaa agaggctgtc
     6121 aaagaatacc aaagattatc aggtattcaa attcgaatga cgtatccatc ggaattaaac
     6181 aaaaaagaga tggaacagtt agaacttctg aatgcggacc tgttcctcta tggttatcag
     6241 ccattgatgg tgtctgcaca atgcgtgaag aataatcttc gaggatgtga taagactcca
     6301 tcttggctga cattaaagga tcgttacaat gcaaacttct tcgttcacac aagttgttgt
     6361 gactgcatca atgagattta taatggaaaa ccattgtggg ttggaaatga agcaggaatt
     6421 cttaatgatc ttcatccatc cgtgatccgt tttcatatta caagagaaaa tgaggtgcag
     6481 gtcaaggaga ttttaaatgc agcgaaatct atacagaaag gggaaagagc ttctcttgcc
     6541 acagaatata cgacagggca tttaaagagg gaagttttat aaaaccatat gatagacatt
     6601 attactacga tagcaaaata tctgatcatc attatgtgtc tgatctatac actgtcatgt
     6661 tatacagtat ttcgtccaaa gaatagagat agaaaggaag acctgttaga caatcaggtc
     6721 atctatatgt ttgtattttt attcttatgt tacacggtac tgtttttgca ggaatttgaa
     6781 ttaaagatct tgatcttttt tgcggcacaa gtgatctttt tcgagatttt gatgattgta
     6841 tttccgagaa tctataaaag atgttcaaga ccgctgatca ataacacctg ttttttgtta
     6901 ggagttgggt ttgtgatctt aacaagatta tcatttgatc tggcaatgaa acagtttgcg
     6961 atcgcggcag gaagtgtgat cgtgacaagt tttattccat tgatgatgca taagatcacg
     7021 atctggaata aatttggatg gctttatgca gtggcaggat ttttactatt gtctacagta
     7081 tttgtgtttg gtatcaacaa gtatggggcg tataactggg tcagcattgc gggacttaaa
     7141 tttcagcctt ctgaatttgt aaagatcatc tttgtgttct ttgttgcagc actactttct
     7201 aaggcaaagg aatttaaaga tctggtaaag atcacagtga tcgcagcact ttatgtatta
     7261 gtgcttgttg ttgagaagga cttaggcgga gcgttgcttt attttgtgat ctatctgatg
     7321 atgctttatg ttgcaaccgc aaaagcaagt tacttatttg gcggtttggc agcaggatct
     7381 gtggctgcta ttatcgcaga taagatcttt actcatgtac agatccgtgt agcggtatgg
     7441 aaagatccgt tttccatgat cgaaggaaga ggacttcagg tctgtcaatc cttatttgct
     7501 attggaactg gaagctggtt tggaatgggg cttggaaatg gacggccgtt tgatattccg
     7561 gtcagggaat cagactttgt cttttctgtg atctgtgaag aatttggtgt gatctttgga
     7621 atctgcctga tcttcgtttt aatgagcagt tttatcctct ttatggatat ttccacaaga
     7681 agcagaaaat tgtttaacaa attgttatgc cttggattcg gagtttgttt cttattccag
     7741 gtattcttaa gtatcggtgg tgtgacaaaa ttcattccat ccactggagt tacgattcca
     7801 ctcgtaagtt atggaggaac ctctgtgatc agtactttga tcatttttaa tatcatacaa
     7861 ggattacata tgttagcaga tagcgaggaa gaagaatatg agcatattaa ggcacaagaa
     7921 tcagaaaaac aatatacaag acaaaacaac cgaaagaatc agtaagataa agaagaaaaa
     7981 gaaaaaaaga gcaaatcgcc agattctgca gattacttat atatttgttg tatcattttt
     8041 ggcattgatc gcttatattg cacattttgt tgcggtagat agtaaaaatg tgatcacgaa
     8101 tgcgcataac cggagattac agtctatggc agagaaagta gtacgaggaa agatcatcag
     8161 tagtgatgga aaagtgctgg cacagacact tactgaaaat ggatcagaga tcagagatta
     8221 tccttatggc agaatgtttg cacatgttgt aggatataac gataaaggaa agtcaggact
     8281 ggaatctgta tgtaactttg atctattaaa atcggatgcg aacttgatga cacagatc
//
LOCUS       DAOMUE010000058         8299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2228_length_8299_cov_2.264920, whole genome shotgun sequence.
ACCESSION   DAOMUE010000058 DAOMUE010000000
VERSION     DAOMUE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2228_length_8299_cov_2.264920"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PLC71_04110"
     CDS             <1..880
                     /locus_tag="PLC71_04110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRM01860.1"
                     /translation="QIHADLAHQHGFLGEGIGIAYLDTGLFPHKDFSPHSTRIAKFVD
                     FVHSKSFSYDDNGHGTHITGIAASSATFGSDYLGIAPKSHIVSLKVLDASGNGVQSAF
                     LQGLDWIHEYHRSYQIRIVNISIGSPGSEDSSASKELLKHVNALWDDGLVVCIAGGNH
                     GPKPYSISIPGNSPKIITVGSSDDNFQMIGRKHFSSGYSGRGPTTSCVMKPDVVAPGT
                     NIFSCSLNNRYTIKSGTSMATPVVSGSFALLLEKYPFYTNKDIKMKLRKNCDKLKTPR
                     HHQGWGQINLKKLMDL"
     gene            complement(894..2042)
                     /gene="hemW"
                     /locus_tag="PLC71_04115"
     CDS             complement(894..2042)
                     /gene="hemW"
                     /locus_tag="PLC71_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRM01861.1"
                     /translation="MKTNTIELYIHIPFCKSKCKYCDFCSFRAEEETIHAYLGKLKEE
                     LIFWGKKLAARDVVTVFIGGGTPSYLRREDIQMICETIFEHFHICEDAEITIEANPGT
                     VDLKKLCTYRENGINRISFGLQSTVKEELEYLGRIHTYEEFLQSFDWARQAGFLNINV
                     DLMSAVPKQTLTSYEENLRKIAKLSPEHISAYSLIIEEGTPFYEDNKLEELLPSEEDE
                     VLMYRMTEKILNEYGYDKYEISNYAKPGFESRHNLGYWSHIPYLGVGLNASSYLDEKR
                     FENPSDMKDYLEIQSFGDAYEGARSLSVYEQMEEFMFLGLRKTKGISKKEFIERFGCS
                     MDSVYGKQLIESMKQGMMKEEGDRVFLTQDGTLVSNQVLCEFLFDEEV"
     gene            complement(2032..3843)
                     /gene="lepA"
                     /locus_tag="PLC71_04120"
     CDS             complement(2032..3843)
                     /gene="lepA"
                     /locus_tag="PLC71_04120"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRM01862.1"
                     /translation="MAIDQSKIRNFCIIAHIDHGKSTLADRIIQMTGTLTDREMQEQV
                     LDNMDLERERGITIKSQAVRIVYKADDGEEYIFNLIDTPGHVDFNYEVSRSLAACEGA
                     ILVVDAAQGIEAQTLANVYLAIDHDLEVMPVINKIDLPSADPERVKAEIEDVIGIDAE
                     DAPQISAKSGLNVEQVLKQIVEKIPSPEGDKNAPLQGLIFDSMYDAYKGVIIFTRIKE
                     GTIKVGTKMKMMATGESAEVVEVGTFGAGRFNPCDELTAGMVGYVTASLKNVQGTRVG
                     DTITDADDPCAEPLPGYKKVNPMVYCGLYPTDGAKYPDLRDALEKLQLNDAALQFEAE
                     TSVALGFGFRCGFLGLLHLEIIQERLEREYNLDLVTTAPGVIYRVYKTDGSMIELTNP
                     SNLPDPSEIDHMEEPIVTAEIMVTSEFVGAIMGLCQERRGVYLGMEYMEETRALLRYE
                     LPLNEIIYDFFDALKSRSRGYASLDYEMKGYQTSRLVKLDILINKEEVDALSFIVHED
                     TAYERGRKMCTKLKEEIPRHMFEVPIQAAIGSKVIARETVKAMRKDVLAKCYGGDISR
                     KRKLLEKQKKGKKRMRQVGNVEIPQEAFMSVLKLDED"
     gene            complement(3896..5023)
                     /locus_tag="PLC71_04125"
     CDS             complement(3896..5023)
                     /locus_tag="PLC71_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HRM01863.1"
                     /translation="MNEYNESMEKRKRWILSITIGCFLIIFFSQKINAQGMVLEFMDH
                     YYHGIMTGFFPAVSKKEMTFSGNILSDMVCMYYQETVPILQYRTDYKDKKEEDLVQQD
                     YYFQDDETTDEVVEEVKKEEKLFHAKKWENSKYLRKYIYQIDSTTMATENELNGKVLL
                     NTNLKLRKSDEPQILIYHTHGSEAYRGSRKGRKSDTVIGVGDVLTKRLEQKNIKVVHD
                     RNIYDVKNGKEERSKAYNYAATAIEKNLKKYPSIQVVIDLHRDGVNESTKLVTRQNGK
                     RMAQIMFFNGMSRTATNGNIKYLKNPNKQTNLAFSLQLQAQAALKYPGFTRKIYVKGY
                     RYNLHYRGRSLLVEVGAQNNTLSEAKASMSLLAELLNNVLY"
     gene            complement(5027..5872)
                     /gene="gpr"
                     /locus_tag="PLC71_04130"
     CDS             complement(5027..5872)
                     /gene="gpr"
                     /locus_tag="PLC71_04130"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HRM01864.1"
                     /translation="MQNRTDLALELKEEKNIGSAQSGIIVKTRIDTTNHIKETKIIIK
                     NKKGEDSLGKPKGTYITIEAKDLSTNDGSFHKEMSEALFLNLKEMLNKKKKILIAGLG
                     NADVTADSLGPKVVNNLYITRHLQKEGIGNYQFELSAIAPGVMAQTGIETSEILESLA
                     DRIKPDVVIVIDALAARSYSRLNKTIQISDTGIAPGSGVGNHRNEITQHTIGVPVLAI
                     GVPTVISVPAIIHDVFGEKSLENVSENIDEEFISMHVTPKNIDESMKRISYTISEGIN
                     HLLHN"
     gene            6069..6332
                     /gene="rpsT"
                     /locus_tag="PLC71_04135"
     CDS             6069..6332
                     /gene="rpsT"
                     /locus_tag="PLC71_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRM01865.1"
                     /translation="MANIKSAKKRIKVINTKTERNKAIKTKVKTYIKKVEAAIAANDK
                     AAASEALTACISEISKATSKGIFHKNTASRKIARLSKAVNAIA"
     gene            complement(6451..7431)
                     /gene="holA"
                     /locus_tag="PLC71_04140"
     CDS             complement(6451..7431)
                     /gene="holA"
                     /locus_tag="PLC71_04140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRM01866.1"
                     /translation="MKKILEDIKTNTYERFYLFYGEEKYMISQMKNQLKKALVSEDDT
                     MNYSYFEGKRADAIEIVELAKTLPFFSDHRFLILDETGLGKKSDDTFIEGLKEASDTS
                     VVLFIEDSVDKRSKIYKFLSKEGHAVCFESAGDKELSRWLISLLKKEGKQMSSATMRS
                     FLYRCGSDMYTLKNELEKLISYVGEREEITIRDLEELTSSQTTNQIFVMLEAIARKQR
                     EKVLTLYYDLIELKESPFGILALLVRQCNQLLQVKDLDCLGKSNGMIAKQMKVPAFVA
                     GKLKDQAKMFSMDTLRDMIEACAKTDESIKTGKISDRVGVELLLIQFSQK"
     gene            complement(7488..>8299)
                     /locus_tag="PLC71_04145"
     CDS             complement(7488..>8299)
                     /locus_tag="PLC71_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM01867.1"
                     /translation="AKKIILQQYRDGIMIILIWIPMARRKSLAFLDVGQGDCFVADTK
                     SGAIIFDGGSSSEDQVGRYRILPYMKYLGYQKIQIAVISHMDIDHYSGIKELLEMGKI
                     KYLGLPEIPKDETMKKIIGIAKKRGTTIFYLSRGRQITTKDGSLKVLHPQKNSQMEKN
                     AASLVMQGKILGYRVLLTGDVEKEGEEELLSEELERADILKAAHHGSKNSTSNEFLQK
                     VQPKQTVISCGKQNRYGHPHLETIHRLQTYRTKIYRTDRSGAIIFFKRRDG"
BASE COUNT         2308 a         1879 c         1192 g         2920 t
ORIGIN      
        1 acaaatccat gctgatcttg cccatcagca tggttttctt ggagaaggta tcggtattgc
       61 ttatcttgat actggccttt tcccacataa agatttctct ccgcattcca cacggatcgc
      121 caaatttgta gattttgttc attccaaatc tttcagctat gacgataatg gacatgggac
      181 tcatattacc ggcatcgctg cttcctctgc aacatttggt tccgattatt tgggaattgc
      241 tccaaaatcc catatcgttt ctttaaaagt actggatgcc tcaggaaacg gtgttcaaag
      301 cgccttctta cagggtcttg actggattca tgagtatcat cgctcctatc agatccgtat
      361 cgttaacatc tcaataggaa gtcctggttc cgaggatagt tcagcctcca aagaactatt
      421 aaaacatgtc aacgccctat gggatgatgg tcttgttgtt tgtattgccg gcggaaacca
      481 tggtccaaaa ccatacagta tttcaattcc tggaaatagt ccaaaaatca tcactgttgg
      541 ttccagcgac gataattttc aaatgatcgg aagaaaacac ttctcttcag gatattccgg
      601 acgcggtccc acaacatcct gcgttatgaa gccggatgtt gttgcccctg gaacaaatat
      661 tttttcgtgt tccttaaata accgctatac tatcaaaagt ggtacttcca tggcaacccc
      721 tgtcgtctct ggttcttttg ctctcctgct cgaaaaatat cctttttaca ccaataagga
      781 tataaaaatg aaactgcgaa aaaactgcga caaattaaaa acccccagac accatcaggg
      841 ctgggggcag atcaatctaa aaaaattaat ggatctttga taaacttgta tccttaaact
      901 tcctcgtcaa ataaaaattc acataagacc tgattactga ctaaggtacc atcttgcgtc
      961 agaaaaactc ggtctccttc ttctttcatc atgccttgtt tcatggattc tatcaattgc
     1021 tttccataga cagaatccat ggagcaacca aaacgttcta taaactcttt cttcgaaatg
     1081 cctttcgtct ttcgaagtcc aagaaacata aattcttcca tctgttcata aactgataac
     1141 gaacgggctc cttcatatgc atctccgaac gattgaatct ctaaatagtc tttcatatcg
     1201 gagggattct caaaacgttt ctcatccaga tacgaagagg cattaagccc cactcccaaa
     1261 tacggaatat gggaccaata cccaagatta tgtctgcttt caaaacctgg ctttgcataa
     1321 tttgaaatct catatttgtc gtaaccatat tcgtttaaaa tcttttctgt cattcgatac
     1381 attaaaactt catcttcttc tgatggaaga agttcctcta acttgttatc ttcgtaaaaa
     1441 ggtgttccct cttcaatgat cagactatac gcagaaatat gttctggaga aagtttagct
     1501 atttttctta aattctcttc ataagaagtt aatgtttgtt tcggcaccgc actcatcaag
     1561 tcaacattga tgttcaaaaa tccagcttgt cttgcccagt cgaaactttg taaaaattct
     1621 tcataggtat gaattcttcc cagatactcc aactcttctt tcactgttga ctgcaagcca
     1681 aaactaatcc tgttgatccc attttcccta tatgtacata gtttttttaa atctactgtt
     1741 cctggattcg cttcaatcgt aatctcagca tcctcacaaa tatgaaaatg ttcaaatata
     1801 gtttcacaaa tcatttgaat atcttctctt ctaagatatg atggcgttcc gcctccaata
     1861 aagacggtta caacatctcg tgctgctaat ttctttcccc agaaaatcag ttcttctttt
     1921 agtttcccca ggtatgcatg gatcgtttcc tcctctgccc gaaaggaaca gaaatcacaa
     1981 tatttgcact tacttttaca aaatggaata tgaatataaa gctcgatcgt attagtcttc
     2041 atctaacttc agaacactca tgaatgcttc ctgtggaatt tcaacatttc ctacctgacg
     2101 cattcgtttc tttcctttct tctgtttctc taacagcttt ctcttacggg agatatctcc
     2161 accgtaacat ttggcaagaa cgtcttttct cattgctttg acagtttctc ttgcaatgac
     2221 tttacttcca atcgctgcct gaatcggtac ttcaaacata tgtcttggaa tttcttcttt
     2281 taattttgta cacatcttac gtccacgctc atacgcggta tcttcatgta caataaaaga
     2341 aagcgcatca acttcttctt tgttgatcaa aatgtctaat tttacaagtc ttgatgtctg
     2401 atatcctttc atttcgtaat ctaaggatgc ataacctctg gaacgtgatt ttaatgcatc
     2461 aaagaaatca taaatgattt catttaatgg aagttcataa cgaagtaatg cccttgtctc
     2521 ttccatatac tccattccaa gatatacgcc tcgtctttcc tggcataatc ccatgatcgc
     2581 tccaacaaac tcagaagtta ccatgatctc tgctgttacg attggttctt ccatatggtc
     2641 gatctcggat ggatctggaa gattggaagg attcgttaat tcgatcatac ttccatccgt
     2701 cttatatacg cgataaataa cgcctggtgc tgttgttaca agatccaagt tatattctct
     2761 ctctaaacgt tcctgaataa tctctaaatg caataatcca agaaatccac atctaaatcc
     2821 aaatcctaac gcaacggatg tctctgcttc aaattgtagt gctgcatcat ttaactgtaa
     2881 tttttctaat gcatctctta aatccggata ttttgctcca tctgttggat atagtccaca
     2941 atataccata ggatttactt ttttgtatcc tggaagtggt tcagcacatg gatcatctgc
     3001 atctgtaatc gtatcaccaa ctctggttcc ctgtacattt ttaaggctgg ctgtaacata
     3061 tccaaccatc cctgctgtca gttcatcaca tggattgaat cttcctgccc caaatgtacc
     3121 aacttctaca acttctgcac tctctccagt tgccatcatc ttcatctttg ttccgacttt
     3181 gatcgttcct tctttgatcc ttgtaaagat gataactcct ttatatgcat catacatact
     3241 gtcaaagatc aatccttgta atggtgcatt cttatcgccc tctggtgatg gaatcttctc
     3301 tacgatctgc tttaatacct gttctacatt tagtccgctt tttgcagaaa tctgaggtgc
     3361 atcttctgca tcgattccga tcacatcttc aatttctgct ttaacacgct ctggatctgc
     3421 actcggaaga tcgatcttgt tgataaccgg cataacttct aagtcgtgat caatcgcaag
     3481 ataaacattt gccaatgtct gtgcctctat tccctgtgca gcatcgacaa caaggattgc
     3541 tccctcacat gctgcaagac ttcgtgatac ttcataatta aagtccacgt gtcccggtgt
     3601 atcgatcaag ttaaaaatat attcttcccc atcgtcagct ttataaacaa ttcgtactgc
     3661 ctgcgatttg atcgtaatcc cacgttctct ttcaagatcc atgttatcaa gaacctgctc
     3721 ctgcatctct cgatccgtta atgttcctgt catctgaatg attcgatctg ctaatgttga
     3781 ttttccgtga tctatatgtg cgataataca aaaatttcgt attttactct gatctattgc
     3841 cataagttcc tcctaattat atttcaagta catatgtctt ttcagacact ccattttagt
     3901 ataacacatt atttaataat tcagcaagta aactcataga cgcttttgct tctgataacg
     3961 tattattctg cgcaccaact tccactaata agcttcttcc tctgtagtgc agattataac
     4021 gatatccttt tacgtaaatt tttcgtgtaa atccagggta tttcaaagca gcctgtgctt
     4081 gcaattgcag gctgaacgca agatttgtct gcttatttgg attctttaaa tatttgatat
     4141 ttccatttgt tgctgtacgg ctcattccat taaaaaacat gatctgcgcc attctctttc
     4201 cattttgtct cgtcacaagc tttgtcgatt cattgactcc atccctgtgc agatcgatca
     4261 caacctgaat ggatggatat ttttttagat tcttttcaat tgcagttgct gcataattat
     4321 aagccttgga tcgctcctct tttccgtttt ttacatcgta aatgttacga tcatgtacaa
     4381 ccttgatatt tttttgctcc aatctcttcg ttaaaacatc tccaacaccg atcacagtat
     4441 ctgattttct tcctttcctg ctgccacggt atgcctcact cccatgtgta tgataaatta
     4501 aaatctgcgg ttcatcagat ttccttaatt ttaagttcgt gtttaataaa acttttccat
     4561 ttaactcatt ttctgtcgcc attgtcgtac tgtcgatctg ataaatatat tttcttaaat
     4621 attttgaatt ttcccatttt tttgcatgaa acaacttttc ttcttttttt acctcctcta
     4681 ccacctcatc cgttgtttca tcatcctgaa aataataatc ttgctgtact agatcttctt
     4741 cttttttatc cttataatct gtgcgatatt gtaagattgg tactgtttcc tgataataca
     4801 tacagaccat gtccgataga atatttcccg aaaatgtcat ttccttctta gatactgccg
     4861 gaaaaaatcc tgtcatgatc ccatgataat aatgatccat gaattctaaa accattccct
     4921 gcgcattgat cttctgactg aaaaaaatga tcagaaaaca accgatcgta atactcaata
     4981 tccatctctt tcttttctcc atactttcat tgtattcatt catattttaa ttatgcagta
     5041 aatgattgat tccttcagat atcgtataac tgatccgttt catagattca tcaatatttt
     5101 ttggtgtaac atgcatggaa ataaattctt catcaatatt ttcagaaaca ttttccagag
     5161 atttttctcc aaaaacatca tgaatgatcg ctggaacaga aattaccgtt ggaacaccaa
     5221 ttgcaagaac tggaacacca atcgtatgct gtgtaatctc atttctatgg tttccaacac
     5281 ctgaacccgg tgcaattccc gtatcgctga tctgaatcgt tttatttaac ctgctataac
     5341 ttctggccgc cagagcatcg atcacgataa caacatctgg ttttatccgg tctgctaatg
     5401 attctaaaat ctcacttgtt tcaattcccg tctgtgccat aacccccggt gcgatcgcac
     5461 ttagttcaaa ttgataattt ccaatcccct ctttttgcaa atgtcttgta atatatagat
     5521 tgttcacaac tttaggccct aacgaatccg ctgtcacatc agcatttcca agtcctgcga
     5581 tcagtatttt tttctttttg tttaacattt cctttaaatt taaaaacaat gcttcactca
     5641 tttctttatg aaaactacca tcgtttgttg acagatcttt cgcttcgatc gtaatatagg
     5701 ttccctttgg ttttcctaaa gagtcctccc cttttttgtt tttgatgatg atctttgttt
     5761 cttttatatg attcgttgta tcaattcttg tctttacgat aattcctgac tgtgcagagc
     5821 caatattctt ctcttctttt aattccagtg ccagatctgt acgattttgc attgattttg
     5881 cctccttttt tctctagtat gtgattgttt ttaaaaaaat attacatttt ttccccaaag
     5941 atattgacaa attcaatcat tttctataag atataacagt atgttttgtg tatagctaac
     6001 cgtgtctcgc tatagacaag attcaagaaa agaaagaaat ttaagttatc atattggagg
     6061 tgcccacttt ggcaaatatt aaatcagcaa aaaaaagaat caaagttatc aacacaaaaa
     6121 cagaaagaaa taaagcaatc aaaactaaag ttaaaacata tatcaaaaaa gtagaagcag
     6181 cgatcgcagc taacgataaa gcagcagctt ctgaagcttt aacagcttgc atttctgaaa
     6241 tcagcaaagc tacttctaaa ggaatcttcc acaagaatac agcttctaga aagattgctc
     6301 gtttatctaa agccgttaac gctattgctt aatcagatat caagaaacga attttaataa
     6361 aattaacccg aaaaaaagaa gccaccgtca ggcttctttt ttttcatcat ttttattata
     6421 ctagacttct tctatcttaa attgattgat ttatttctga ctgaactgga tcaacaatag
     6481 ttctacccca actctgtcac tgatctttcc tgttttaatt gattcatcag tttttgcaca
     6541 tgcttcgatc atatctctga gtgtatccat tgaaaacatt ttcgcttgat cttttaactt
     6601 tcctgcaaca aatgctggaa ctttcatctg ctttgcgatc atcccattgc tctttccaag
     6661 acaatctaaa tctttcacct gaagcagctg attacactgt ctgactaaca atgccagaat
     6721 tccaaatggt gattccttta actcgatcag atcataatat agtgtcaata ctttttctct
     6781 ttgctttctc gcaattgcct caagcatcac aaagatctga tttgtcgtct gagaagatgt
     6841 caactcttcc agatcacgga tcgtaatttc ttctctctct ccaacatagg agatcaattt
     6901 ctctaattca ttttttaaag tatacatatc agatccacag cgatataaaa aacttcgcat
     6961 cgttgcagat gacatctgtt ttccttcttt cttaagcaat gagatcagcc atctggataa
     7021 ttctttatct ccagcagatt caaaacatac cgcatgtcct tccttcgaaa gaaatttata
     7081 aatctttgaa cgcttatcta cactatcttc tataaaaagc acaacacttg tatccgaggc
     7141 ttcctttaat ccttcaataa acgtatcatc acttttcttt ccaagtcctg tttcatccaa
     7201 gatcaaaaac ctatgatcac taaaaaacgg cagtgtttta gcaagttcaa caatctctat
     7261 cgcatcagcc ctttttcctt caaaataaga atagttcatc gtatcatctt ctgaaactaa
     7321 tgctttcttt aactggtttt tcatctggga gatcatatat ttttcttccc cgtagaatag
     7381 ataaaaacgt tcgtatgtat ttgtcttgat atcttctaat attttcttca tattctttgc
     7441 atctctttct atagcttttg atagtatacc ttttttttgg aagattttca accatctctt
     7501 cttttgaaaa aaataatcgc accacttcga tctgttctat aaatctttgt gcgatatgtc
     7561 tgtagtctgt ggatagtttc taaatgtggg tgtccatatc tattttgttt tccacaagag
     7621 atgaccgtct gtttcggctg aaccttttgt aaaaattcat tactcgtgga attttttgac
     7681 ccatgatgtg ctgcttttaa tatatctgct ctttccaatt cttctgataa tagttcttct
     7741 tcgccttctt tttccacatc tcccgttaac agcacccgat atccaagtat ttttccctgc
     7801 attaccaacg atgccgcatt cttttccatc tgactgttct tttgaggatg caacactttc
     7861 aaacttccat cttttgtagt gatctgcctt cctcttgaaa gataaaaaat agttgttcct
     7921 cgtttcttcg ctatacctat gatctttttc attgtctcat cttttggaat ctctggaagc
     7981 ccaagatact taattttccc catctcaagt aattccttga tcccagaata atgatcgata
     8041 tccatatgac tgatcaccgc aatctgaatc ttctgatacc ccagatattt catatacgga
     8101 agaatccgat atcttccaac ctgatcttca ctgctgcttc caccatcaaa tatgatcgcc
     8161 ccagacttcg tatctgctac aaaacaatct ccctgcccaa catcaagaaa tgccagactc
     8221 tttcttcttg ccattggaat ccatatcaaa atgatcatga taccatcccg atactgctgc
     8281 aaaataatct tctttgctg
//
LOCUS       DAOMUE010000059         8076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2319_length_8076_cov_2.409425, whole genome shotgun sequence.
ACCESSION   DAOMUE010000059 DAOMUE010000000
VERSION     DAOMUE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2319_length_8076_cov_2.409425"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2005)
                     /locus_tag="PLC71_04150"
     CDS             complement(<1..>2005)
                     /locus_tag="PLC71_04150"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HRM01868.1"
                     /translation="GIGSQEEVTCNGYSIQTRITTEDPMNNFLPDTGKITVYRSGAGN
                     GIRLDGGNAYAGAEITPYYDSLLVKAISHDRTFGRAVDKSIRVLKEIRIRGVKTNIPF
                     LINVLNNETFREGRCYTTFIEETPELFLLPESQDRATKILEFLGNKMVNVQKAVLDKP
                     DFEARTLPKYDTEKKIYGSRDKFLEMGAKDFTQSLLNEKRLLITDTTMRDAQQSLMAT
                     RMRTKDLIGASDATNAFMENAFSVEAWGGATYDTAYRFLKESPWKRLKLLRQHMPNTL
                     IQMLLRASNAVGYSNYPDNVVKKFIEEASQKGVDVFRIFDSLNWVENMKMPIETALKT
                     GKIVEGTICYTGDITDPNETKYTLDYYVKKAKELESLGVHIFTIKDMAGLVKPYAAKK
                     LISALKEELNIPVNLHTHDSTGNGVSTLLMASEAGLDIADCAIGSMSSMTSNPCMNSL
                     VEALKGTERDTGLNPDELTELSQYYARIRPIYKQFESGMDAPNTEIYKYEIPGGQYSN
                     LLAQVTEMGSPEEFEAIKGLYKEANDLLGNIVKVTPTSKAVGDLAIFMYKNNLNKDNI
                     LTAGASLSYPDSVVSYFQGMMGQPYGGFPKELQKIVLKDIEPLTERPGKLLPPEDLEG
                     IKKHLIEKYHYEDKSEEVMAQKAISYALYPKVYEDYCEHFEMYN"
     assembly_gap    2006..2015
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2016..>2473)
                     /locus_tag="PLC71_04155"
     CDS             complement(<2016..>2473)
                     /locus_tag="PLC71_04155"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568911.1"
                     /note="biotin-containing enzyme that catalyzes a two step
                     carboxylation of pyruvate to oxaloacetate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HRM01869.1"
                     /translation="HSAEEMPKEYAEARDESKKAFGDDQIFVEKYLKSPKHIEVQIIG
                     DQYGHVVHLYDRDCSVQRRHQKVVEYAPAFSVPEEVRQQIFEASIRLAKHVGYKNAGT
                     LEFLVDADNNAYFIEMNPRVQVEHTITEMVTGIDIVQTQVLVAEGYSLDS"
     assembly_gap    2474..2483
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2484..2991)
                     /locus_tag="PLC71_04160"
                     /pseudo
     CDS             complement(2484..2991)
                     /locus_tag="PLC71_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202818.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
     gene            complement(3127..5025)
                     /locus_tag="PLC71_04165"
     CDS             complement(3127..5025)
                     /locus_tag="PLC71_04165"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HRM01870.1"
                     /translation="MEVILAKTAGFCFGVKRAVDTVYKEAGKKNVYTYGPIIHNSEVV
                     NDLKKKGVEVINSREELEALEEGTVIIRSHGVAREIYDLIHEKGLELVDATCPFVRKI
                     HKIVEKAGNDGDQVIIVGSEHHPEVQGIKGWCTGEVHIISDAEQFEGIDLNKPTTLVS
                     QTTFNYKKFQDLVEILNKKGYDIGVCNTICNATEERQLEAKSIAKGVDAMVVIGDKQS
                     SNSQKLYEISKKECENTFFVQTLRDLDLKLFESTGKVGITAGASTPQKIIKEVHASMT
                     EKSFEELLEESFVTIHNGEVVKGTVIDVKPDEIILNIGYKADGILTRSEYSNDSANVD
                     LTTVAKVGDTMETKVLKVNDGEGQVLLTYKRLAAEKGNKRLEEAFENKEVLKAPVAQV
                     LDGGLSVLIEEARVFIPASLVSDSYERDLKKYEGQEIEFVISEFNPRKRRVIGDRKQL
                     LVAAKKEKQKELFEKIEAGMKVEGVVKNVTDFGAFIDLGGADGLLHISEMSWGRVENP
                     KKVFNIGDKVTVLIKDIQGEKIALSLKFPEENPWLKAEEKYKVGSVVTGKVARMTDFG
                     AFVELESGIDALLHVSQIAKEHIDKPSDVLSVGQEIEAKVVDFKKDDKKISLSMKALA
                     NEEEKTEE"
     gene            complement(5059..5721)
                     /gene="cmk"
                     /locus_tag="PLC71_04170"
     CDS             complement(5059..5721)
                     /gene="cmk"
                     /locus_tag="PLC71_04170"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRM01871.1"
                     /translation="MYSIAIDGPAGAGKSTIAKTIAKKLEFIYVDTGAMYRAMALYFI
                     RHEIDPKDEEAINKACPDIHVSINYEDGVQQVLLNGENVNGLIRTEEVGNMASKTSAY
                     KMVRATLLDLQRDLAKTANILMDGRDIGTNVLPNADLKIYLTASSKVRAERRYKELVE
                     KGVEADFDKIEEDIIIRDRQDMEREIAPLKQAEDAVLVDSSDMTIEEVVDAIVAEFEK
                     VK"
     gene            complement(5754..6971)
                     /locus_tag="PLC71_04175"
     CDS             complement(5754..6971)
                     /locus_tag="PLC71_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM01872.1"
                     /translation="MKVIIIGGGAAGMMAAYQAAKCGNDVSLYEKNEKLGKKIFITGK
                     GRCNVTNACEIEELLDHVVTNKEFLYSGFYSFTNDAMMEFLEELGCPLKVERGNRVFP
                     VSDHSSDVIAALQRGLRKENVDIYFNTTVKKILIEDGIVTGVRLASGDTVKADKVVVA
                     TGGMSYQATGSTGDGYDFARKAGHKVTKLTPALVPFEIKEDYCKQLMGLSLRNVAVTM
                     TADGKKIYDDFGEMLFTHFGISGPVVLSASSYIGRYQGKELKFIIDLKPALNKEQLDH
                     RVLKDFNKTLNKDFVNSLDHLLPKKLIPVMIELSGIPEHKKVNEITKKERERFVSLLK
                     HLELTITGVRGFKEAIITQGGVNVKEIDPGTMESKKIKGLYFAGEVLDLDAVTGGFNL
                     QIAWSTGYLAGMN"
     gene            complement(7000..8028)
                     /locus_tag="PLC71_04180"
     CDS             complement(7000..8028)
                     /locus_tag="PLC71_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM01873.1"
                     /translation="MKLALIGTGKIIEDALFAMEPVETIERTAIFARPHSRDKAEKFA
                     KQYAIKEVYTDYEELLEKTEADTVYIGLINSAHYPYAKQALLAGKNVILEKPFTGFYE
                     ETKDLEQIANDKKLFIFEAITVLHNEVFYEMKKNLNKLGNIRMALCNYSQYSSRYDAY
                     LEGDITHSFDPAYYGGSLYDINVYNIHYCVGLFGEPKDATYYPNIGPNGIDTSGTLVM
                     EYDGFSAVCTGSKDSDSPGYVCVQGEKGFMKVDSKPNIASELKTVYADENIKEKVKDA
                     AGAMVRATITEDYKAPEHHHRMTQEFTDFARVIDEKDYETAKEFLDETVAVVKVLETA
                     RKKAGIEF"
BASE COUNT         2120 a         1833 c         1214 g         2889 t
ORIGIN      
        1 attatacatt tcaaaatgtt cgcagtaatc ttcatatacc ttcggataca aggcatagct
       61 gattgctttt tgtgccataa cttcttctga tttatcctca taatggtatt tttcaatgag
      121 gtgctttttg ataccttcca aatcttctgg cggcaataat ttacctgggc gttctgtaag
      181 cggttcaata tcttttaaca caattttctg taattctttc gggaatccac cataaggctg
      241 tcccatcatt ccctggaaat aagaaacaac ggaatctgga taagacagac tagctccggc
      301 agttaaaata ttatccttat tcagattatt tttatacatg aaaattgcaa gatcacctac
      361 cgcttttgaa gtaggggtta cttttacaat attaccaagc aaatcattcg cttctttata
      421 aagccctttg atcgcctcaa attcttccgg agatcccatt tctgtaacct gcgcaagaag
      481 attagaatat tgtccacctg gaatctcata tttgtaaatc tctgtatttg gtgcatccat
      541 tccgctctca aactgtttgt agattgggcg gattcttgca tagtactgac ttaattctgt
      601 taattcatct ggattaagtc ctgtatctcg ctctgttcct tttaatgctt ctactaatga
      661 attcatacat gggttacttg tcatagaact catagaaccg atcgcacagt cagcgatatc
      721 tagtcctgct tcagatgcca ttaataatgt gctgacacca tttccagtag aatcatgtgt
      781 atgaagatta actggaatat ttaattcttc ttttaatgca ctaataagtt tcttcgctgc
      841 atatggtttt acaagacctg ccatatcttt gatcgtaaag atatgaacac ctaagctttc
      901 taattctttg gctttcttta catagtaatc taatgtatat tttgtctcgt ttggatctgt
      961 gatatctcct gtgtagcaga tcgttccttc tacgatctta cctgtcttta atgctgtctc
     1021 gatcggcatc ttcatattct caacccagtt taagctgtcg aaaatacgga acacatctac
     1081 acctttttgt gatgcttctt cgatgaattt cttaacaaca ttatctggat agttgctgta
     1141 tcctacagca ttggatgctc gaagtaacat ctggatcaat gtatttggca tatgttgtct
     1201 taataatttt aatctcttcc atggagactc ttttaagaaa cgataagctg tatcataagt
     1261 agccccaccc catgcctcta cagagaatgc attctccata aatgcgtttg tcgcatcaga
     1321 tgctccgatc agatcttttg ttctcatacg tgttgccatt aaagactgct gtgcatcacg
     1381 cattgttgta tctgtgatca gtaatctctt ctcatttaac aggctctgtg tgaagtcttt
     1441 agctcccatc tctaagaact tgtctctgga accataaatc ttcttctccg tatcatactt
     1501 tggaagagtt cttgcttcaa aatctggttt gtcaagaacg gctttctgca cattcaccat
     1561 cttatttcct aaaaattcaa ggatctttgt tgctctatcc tgactttctg gaagtaagaa
     1621 taattctggc gtttcttcaa taaatgttgt atagcatctt ccctcacgga atgtctcatt
     1681 atttaataca ttgatcaaga atgggatatt tgtcttaact ccacgaatac ggatctcttt
     1741 taatacacgg atagatttgt caaccgctct tccaaatgta cgatcatgag agatcgcttt
     1801 taccaataag ctgtcatagt atggtgtaat ctctgctcct gcatatgcgt taccaccatc
     1861 taatcgaata ccgttaccag ctccagaacg atataccgtg atcttacctg tgtctggcag
     1921 gaagttattc attggatctt ctgttgtgat acgtgtctga atagagtatc cattacatgt
     1981 cacttcctcc tgagatccaa taccgnnnnn nnnnntcaga atctaaagaa tatccttctg
     2041 ctacaagaac ctgtgtctgt acgatatcga ttcctgttac catctctgtg atcgtatgtt
     2101 ctacctgtac acgagggttc atctcgataa agtatgcatt attatctgca tctaccagga
     2161 actctaatgt tccagcattc ttatatccta catgttttgc aagacggata gatgcttcaa
     2221 aaatctgctg tcttacttct tctggcacgc tgaatgctgg tgcatattct acaaccttct
     2281 gatgtcttct ctgtacagag cagtcacggt catataaatg aacaacatgt ccatactgat
     2341 caccaatgat ctgaacctcg atatgttttg gactctttaa gtatttttca acgaaaatct
     2401 gatcgtctcc aaatgctttc ttggattcat ctcttgcttc tgcatattct tttggcatct
     2461 cttctgctga gtgnnnnnnn nnncaccatt agaggccttt aacatcacag gatatccaac
     2521 ctggttcgca atcttagtca cttcttccag tccgtgaacc gcatggtcta caccaggaat
     2581 gattggaaca tcagcttcaa ttgccatctg tttggaagaa atcttatctc ccatcttacg
     2641 cattaagctt gaggatggtc cgataaatgt gattccattc ttctcacagg catctacaaa
     2701 gtcaggattc tctgataaaa atccataacc tggatgaatt gcatctacgt tcttctcttt
     2761 tgcaatacga atgatcgtat tgatgtcaag ataggcatcg attggtcctt tgtccggatt
     2821 caactgataa gattcatcag ctttagaacg gaataaggca taacggtctt cttttgaaaa
     2881 gacaccaacc gttgtaattc caagttcatt taaagctcgg aaaactcgga tcgcaatctc
     2941 accacggttt gcaaccaata cttttttaaa cttctttaat tccatggtca taactccttt
     3001 tcgaggttta tcattttaaa aaaatgtatt tttaaaactg attatatcgc aaaacttgtt
     3061 tttttacaat acttgtacga aaaaagaacc ctgatttata aaaatcagga ttctttgata
     3121 aagatcttat tcttctgttt tttcttcttc gttagctaat gctttcatac ttaagctgat
     3181 cttcttatcg tctttcttga agtcaactac tttagcttcg atttcctgtc caacacttaa
     3241 tacgtcagat ggtttgtcga tatgttcttt tgcgatctga gaaacgtgaa gaagtgcatc
     3301 gattcctgat tctaactcaa cgaatgctcc gaaatctgtc atacgtgcaa ctttacctgt
     3361 tacaacactt cctactttgt atttctcttc tgcttttaac catggattct cttctgggaa
     3421 ttttaagctt aatgcaatct tttctccctg aatatccttg atcagaactg ttactttatc
     3481 tccaatattg aagaccttct tagggttttc aacacgtccc caagacattt cagaaatgtg
     3541 aagaagtccg tctgctccac ctaaatcgat gaatgcaccg aagtctgtta cattcttaac
     3601 aactccttct actttcattc ctgcttcgat cttctcgaat aattctttct gtttttcttt
     3661 cttagcagct actaataact gtttgcggtc tccgatgact cttctcttac gaggattgaa
     3721 ctcactgata acgaattcga tttcctgtcc ttcgtatttc tttaagtctc tttcataaga
     3781 atcagaaact aagcttgcag ggatgaatac acgtgcttct tcgatcagta cgcttaatcc
     3841 accatctaaa acctgagcta caggtgcttt taagacttct ttattctcga aagcttcttc
     3901 taaacgtttg tttccttttt cagctgctaa tctcttataa gtaagaagaa cctgtccttc
     3961 tccatcattc acttttaata ccttagtctc catagtatca ccaactttag caacagttgt
     4021 taaatctacg ttggctgaat cattagagta ttcacttctt gttaagatac catctgcttt
     4081 gtatccgatg tttaagatga tctcatctgg tttcacatcg ataactgtac ctttcactac
     4141 ctctccgttg tgaattgtca caaaactttc ctctaataat tcttcaaaac tcttttctgt
     4201 catgctagca tgaacctcct tgataatttt ctgtggggtt gaggcccccg ctgtaatacc
     4261 taccttacca gtcgattcaa aaagtttcaa atccaggtct ctaagtgtct gcacaaaaaa
     4321 ggtgttctca cactctttct tactaatctc atataacttc tggctgttgg agctttgctt
     4381 atctccgatc acaaccattg catctacacc cttagcgata gactttgctt ccagttgacg
     4441 ctcttccgtg gcattacata ttgtattaca tactcctata tcataaccct ttttattcag
     4501 aatttcaact aaatcttgaa atttcttgta attaaatgtc gtttgtgata caagagtcgt
     4561 cggcttgtta agatcgatcc cttcaaactg ctctgcatct gagataatat gcacttctcc
     4621 agtacaccat cctttgattc cctgaacttc tgggtggtgc tcacttccaa cgatgatcac
     4681 ctgatcacca tcgttgcccg ctttctctac gatcttatgg atctttctga caaatggaca
     4741 tgttgcatca actaattcca atcctttttc atggatcaga tcataaattt ctctcgcaac
     4801 tccatgggaa cgtatgataa ccgttccttc ttccaaagct tctaattctt ctctggaatt
     4861 gatcacttcc acgccctttt tcttcagatc attaacaacc tctgaattgt gaatgatcgg
     4921 cccgtatgtg tatacatttt tctttcctgc ttctttatat acagtgtcaa cagcacgctt
     4981 cacgccaaaa caaaaccctg ctgtttttgc taaaataact tccatcttta tcctcactgc
     5041 ttgtaaataa actttttatt attttacttt ttcaaattct gcaacgatcg catcaacaac
     5101 ttcttcgatc gtcatatcag aagaatcaac cagcactgca tcttctgcct gttttagcgg
     5161 tgcgatctca cgttccatat cctgacggtc acggataatg atatcttcct caatcttatc
     5221 gaaatcagcc tctacacctt tttctaccaa ttctttgtag cggcgctcgg ctcttacttt
     5281 agatgatgca gttaaataaa tctttaaatc ggcatttggc agtacgtttg ttccgatatc
     5341 tcttccatcc attaagatat tagccgtctt ggcaagatct ctctgaagat ctaatagtgt
     5401 tgctcttacc attttatatg cagatgtctt cgaagccata tttccaactt cctcggtacg
     5461 gatcagtcca tttacatttt ccccgtttaa taacacctgc tgcactccat cttcatagtt
     5521 gatacttaca tgaatatctg gacatgcttt gttgatcgcc tcttcatcct ttggatcgat
     5581 ctcatgtctg atgaaataaa gagccatcgc acggtacatt gctcctgtat ccacataaat
     5641 aaattctaat ttctttgcaa ttgtttttgc aatcgtgctt tttcctgctc ctgctggtcc
     5701 atcaatcgca atactataca ttatcttttc ctcctgatct gcttcctaat atattaattc
     5761 attcctgcaa gatatcctgt tgaccatgcg atctgaaggt taaacccacc tgtgaccgca
     5821 tcaagatcta atacttcccc tgcaaaatat aatcctttga tcttctttga ttccatcgtc
     5881 cccggatcga tctctttgac gttaactccg ccttgtgtaa tgatcgcttc tttaaatcca
     5941 cgaactcctg taattgtaag ttctaaatgc tttaataaag aaacaaatcg ttctctttcc
     6001 ttctttgtga tctcgttaac cttcttatgc tctggaattc ctgataactc tatcataaca
     6061 ggaatcagct tcttcggcaa aagatgatct aaggaattta caaaatcttt gtttaatgtc
     6121 ttattaaaat cttttaatac tctgtgatct aactgttctt tgttaagtgc tggtttcaga
     6181 tcaatgataa attttaattc tttgccctga tatcttccaa tataactact tgcacttaaa
     6241 acgactggtc cactgattcc aaagtgtgta aagagcattt ctccaaaatc atcgtaaatc
     6301 ttcttgccat ctgcagtcat tgtcactgcg acatttctta aagataatcc catcaactgt
     6361 ttacaataat cttctttgat ctcaaacgga acaagtgcag gcgtaagttt tgtcacttta
     6421 tgtcctgctt ttcttgcaaa atcatatcca tctccagtcg atccggttgc ctgataggac
     6481 atacctcctg ttgcaacaac gactttatct gccttcactg tgtctccaga tgccagtctt
     6541 actcctgtta caattccatc ttcgatcaga atctttttga cagttgtatt gaaatatatg
     6601 tcaacatttt cttttctaag acctctttgt aatgctgcga taacatctga agaatgatca
     6661 gatacaggga atactcgatt tcctcgttcc actttcagag ggcatccaag ttcttccaag
     6721 aattccatca ttgcatcatt ggtaaaagaa tagaaaccac tgtataaaaa ttccttgttg
     6781 gttacaacgt gatctaacag ctcttcgatc tcacaggcat ttgtcacatt acagcgtcct
     6841 tttcctgtga taaaaatctt ctttcctaat ttttcatttt tttcatataa ggaaacatcg
     6901 tttccacatt ttgctgcctg atatgcagcc atcatgccgg cagctccgcc accgataatg
     6961 atcactttca tagattactt ccttctattt aatttaattc taaaattcaa ttccagcttt
     7021 ctttcgtgct gtctcaagta ctttcacaac cgcaactgtc tcatccaaaa attcttttgc
     7081 cgtctcataa tctttttcat caatgactct tgcaaaatct gtaaattcct gtgtcatgcg
     7141 gtggtgatgc tctggagcct tataatcttc tgtgatcgtt gcacgaacca tcgctcctgc
     7201 tgcatctttt actttttctt tgatattttc atctgcataa accgtcttta attcagaagc
     7261 aatgtttggt ttagaatcta ctttcataaa acctttttca ccctgaacac agacgtatcc
     7321 tggactatct gaatctttac ttcctgtaca aaccgcactg aatccatcat attccatcac
     7381 aagtgttcct gatgtatcaa ttccatttgg tccgatattt ggataatatg tcgcatcctt
     7441 tggctctcca aataatccta cacaataatg aatattatag acgttgatat catataatga
     7501 acctccataa tatgcaggat caaaagaatg tgtgatatct ccttctaaat atgcatcgta
     7561 acggctggag tactgagaat aattacaaag tgccatacgg atatttccca gtttattcag
     7621 gttctttttc atctcataaa acacttcgtt gtgaagtact gtgatcgctt caaagataaa
     7681 taatttcttg tcatttgcaa tctgctctaa atcctttgtt tcttcataaa atccagtaaa
     7741 tggcttctcg aggatgacat tctttcctgc caataatgcc tgttttgcat atggataatg
     7801 agcactgttg atcagtccta tgtaaacagt atctgcttct gtcttttcaa gtaattcttc
     7861 atagtctgtg taaacttctt tgatcgcata ctgttttgca aatttttctg ccttatccct
     7921 gctatgtggt cttgcaaaaa tcgctgttct ctcaattgtt tctactggtt ccattgcaaa
     7981 caatgcatct tcaataatct ttcctgttcc aataagtgca agtttcataa gctttcctcc
     8041 atttcatatc ttataataat gtttctatat cttttt
//
LOCUS       DAOMUE010000060         8023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2333_length_8023_cov_3.154367, whole genome shotgun sequence.
ACCESSION   DAOMUE010000060 DAOMUE010000000
VERSION     DAOMUE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2333_length_8023_cov_3.154367"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PLC71_04185"
     CDS             <1..688
                     /locus_tag="PLC71_04185"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HRM01874.1"
                     /translation="MQDYIDHWKELNIDFDCIYSGFLGSERQIEIVSGFIDDFGTEDN
                     MVVIDPVLGDNGNLYSTMDQGLVEGMKRLVTKADIITPNFTEVSLLLGEEYKQTTTDE
                     EIKEWVKRLSDMGPDIIVVTSVPDKEKDKDSNVIAYDRTCNTYWKIKCRYIPTFYPGT
                     GDAYTSVMIGSLLDGDSLPIALDKGVQFITQAIKASYGFDYPNREGVLLERVLEVLKM
                     PVVLGGYEMI"
     gene            719..1366
                     /locus_tag="PLC71_04190"
     CDS             719..1366
                     /locus_tag="PLC71_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HRM01875.1"
                     /translation="MFYLLDPEKWERQKHFEYYMQMIPCGYTVTVRLDVENLYQQVKK
                     CGLKFYPAFIYCTGKVVNEKREFKMGVDQEGRPGYFDVMHPNFTIFHKDDHTFSDIWS
                     YYDDDFQTCYQNIINDMETYKDVKGPKGKEGQPSNFFCISCVPWMDFTGYSTWVPTGR
                     PNLFPIITFGKYVEEEGRRKLSAALTISHASADGYHASMFFERLQEVLDEFVIDQ"
     gene            complement(1457..2032)
                     /locus_tag="PLC71_04195"
     CDS             complement(1457..2032)
                     /locus_tag="PLC71_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HRM01876.1"
                     /translation="MVEKKTDRRILKTKRALRESLLYLLKEQPIQKISVSRLCEKSDI
                     NRSTFYTYYSSPMDLLESIEDEILNTLEEDMIQFEKENSISQLMNSIIFYISEHKQLV
                     RLLFSDHGDPGFQNKLLLATQHWTMPMWQSRRPDYDAEALSSLHIYIVSGCMAVIQQW
                     ITGGFKESEEEISSMLEKLSASTSAGFLQGK"
     gene            2200..>3367
                     /locus_tag="PLC71_04200"
     CDS             2200..>3367
                     /locus_tag="PLC71_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM01877.1"
                     /translation="MNRQDFYDGKLFDAYRYMGAHKDGDKIRFVTYAPRASRISIIGE
                     FNNWNEEFMEQDAQSGFFLFYSDKAKEGQMYKYCIYGPDGNRQEHCDPYGFEMELRPG
                     ACSIIRDITTYRFNDRSWMQMRSVGMEDAINIYEMHMGSWRMNKKDENGWYQYDEIAK
                     MLVPYLKQNNYTHVELMPLSEHPFDGSWGYQNTGFFAPTKRYGTPDKLMKFVDMMHQA
                     GIGVIMDFVPVHFAVDGYGLKLYDGTPLYEYDNKDTGESEWGSCNFIHSRREVQCFLQ
                     SAANYWLEEYHFDGIRMDAVSRLIYWQGDESRGVNDTAIDFLKAFNLGLKQRHPTAML
                     IAEDSTAYPGVTEPVWKGGLGFDYKWDLGWMHDTLEYFQTGPEYRSRDYHKLTFS"
     assembly_gap    3368..3377
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3378..4014
                     /locus_tag="PLC71_04205"
     CDS             <3378..4014
                     /locus_tag="PLC71_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HRM01878.1"
                     /translation="VYFWNERYMLEYCHDEVVHGKATILQKMYGDYEDKFPQARAMYM
                     YMMIHPGKKLNFMGNEFGQIREWSEAQEQDWMILKYPIHDAFHKYMVKLNDIYKKEKA
                     FHYDYHRENFEWLDCHQEERCIYAIGRKTADHMIVGIFNFSDKEQKDYKLTIKGHHTR
                     RTLLHTEWEQYGGTMKKRKENIHLKKKGKDSELTITLPRYSGVLLKLTKKE"
     gene            4047..4967
                     /gene="trxB"
                     /locus_tag="PLC71_04210"
     CDS             4047..4967
                     /gene="trxB"
                     /locus_tag="PLC71_04210"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRM01879.1"
                     /translation="MKIYDLVIIGSGPAGMSAAIYAQRAMLDFVMIEKEYMPGGQVVQ
                     TYEVDNYPGIPKTNGMDLGLKFSEHAKELGAKSITAEVKEIYADEEIKEIMLKNGDTL
                     KTRTIILATGAVHQTLGIEGEEKLKGMGVSYCATCDGAFFRNKVTAVVGGGNVALEDA
                     IFLSRMCKKVYLIHRRDEFRGAKILQDEVKKNDKIEILWNTIVTKIDGDEVVEGLHIQ
                     DVDNHVDKLLDVDGIFIAVGTKPASELLSGKLSTDEKGYIIAGEDGVTNIPGIFAAGD
                     GRSKNLRQVVTAVSDGANCVYSVERYLQNA"
     gene            5333..5863
                     /gene="raiA"
                     /locus_tag="PLC71_04215"
     CDS             5333..5863
                     /gene="raiA"
                     /locus_tag="PLC71_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRM01880.1"
                     /translation="MKFIISGKNIEVTEGIKAAIEEKLGRLDKYLVDDTIVNVTLSVQ
                     KGRQKIEVTIPMKGHIIRAEEGSEDMYVSIDLVVDVIERQIRRYKTRLMNQKYDGTSF
                     KQEFMEQEDDVEDDEIRIIRSKKFAIKPMDVEEACVQMELLGHDFFVFRNADTFEVNV
                     VYKRKGNTYGLIEPEF"
     gene            6101..>8023
                     /gene="secA"
                     /locus_tag="PLC71_04220"
     CDS             6101..>8023
                     /gene="secA"
                     /locus_tag="PLC71_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRM01881.1"
                     /translation="MGLFDKVFGSHSDKELKRISKTVDKIEALDESMQKLSDEELRHK
                     TVEFKERLANGETLDDLLPEAYATVREASSRAIGLKHYRVQLIGGIILHQGRIAEMRT
                     GEGKTLVSTLPAYLNALEEKGVHIVTVNDYLATRDAEWMGKVHNFLGLTVGVVTNDME
                     NDERREAYNCDITYITNNELGFDYLRDNMVIEKEDLVQRDLHYAIVDEVDSVLIDEAR
                     TPLIISGESGKSTELYRACDILARQMERGSSDGELSKMDILMNEDIEEDGDFLVNEKD
                     KQIMLTADGVKKVEKFFHIENLADPENLAIQHNIILALRAHNLMFKDKDYVVKDDEVL
                     IVDEFTGRIMPGRRYSDGLHQAIEAKENVKVKRESKTLATITFQNFFNKYDKKAGMTG
                     TALTEEQEFREIYGMDVVVIPTNKPVQRIDHDDAIYKTKREKMNAVVEDIIESHAKGQ
                     PVLVGTITIEMSEELSAMLKKRGIKHNVLNAKFHEKEAEIISHAGEIGAVTIATNMAG
                     RGTDIVLEDGVAELGGLKIIGTERHESRRIDNQLRGRAGRQGDPGESKFYLSLEDDLM
                     RLFGSERMISIYNALGIPEGEEIQHKTISKTIEKAQKKIENNNFGIRRNLLDYDQVNN
                     DQREVIYAERRRVLNGE"
BASE COUNT         2822 a         1235 c         1768 g         2188 t
ORIGIN      
        1 tatgcaggac tatatcgatc attggaaaga gctaaatatc gattttgact gtatttattc
       61 tggattctta ggatctgaac gtcagattga gatcgtttcc ggatttattg atgattttgg
      121 aacagaggat aatatggttg tgatcgatcc agtccttgga gataatggaa acttgtattc
      181 aacgatggat caaggattag ttgaaggaat gaaacgtctg gtaacaaaag ctgacattat
      241 tacacccaat tttacagaag taagtctttt attaggggaa gaatacaaac agacaacaac
      301 cgatgaagag atcaaagaat gggtaaaacg tttgtctgat atgggaccag atatcattgt
      361 tgtgacaagt gttccagata aagagaaaga taaagattct aatgtgatcg cttacgatag
      421 aacttgtaat acatattgga agataaaatg ccgttatatc ccaacatttt atccgggaac
      481 aggagatgct tatacttctg ttatgatagg aagcctgtta gacggagaca gtctgccaat
      541 cgcactggat aagggtgtgc agtttatcac acaggcaatc aaggcaagtt atggatttga
      601 ttatccaaac agagaaggag tattgcttga gagagtattg gaagtattga aaatgccggt
      661 agttttaggt ggatatgaaa tgatttgata tcgaaagcta aaaaaggagg tgtctgacat
      721 gttttattta ctagacccag aaaaatggga acgacagaaa cattttgaat attatatgca
      781 gatgatacca tgcggttata ctgttacagt acgtctagat gtggaaaatc tgtatcagca
      841 ggtcaaaaaa tgtggattaa agttttatcc ggcatttatt tactgcacag gaaaagttgt
      901 taatgagaaa agagaattta aaatgggagt cgatcaggaa ggaagacctg gttattttga
      961 tgtgatgcat ccaaacttta cgatctttca caaagatgat catacatttt cagacatctg
     1021 gtcttattat gatgatgatt ttcagacatg ctatcaaaac attataaatg atatggaaac
     1081 atacaaagat gtcaaaggac ccaaaggaaa agaagggcag ccgtcaaact ttttttgtat
     1141 ctcctgtgtg ccatggatgg atttcacagg ctacagcaca tgggtgccaa ctggaagacc
     1201 aaatctattt ccgatcatta catttggaaa atatgtggaa gaagagggca gaagaaagct
     1261 gtctgctgct ttgacgatat ctcatgcatc cgcagatgga tatcatgcta gtatgttctt
     1321 tgaaagactg caggaagtat tagatgaatt tgtaattgat cagtaattga tgcattgttc
     1381 aaagtttata attcattttt aagtgaaaaa aatcgatgat tataatagca gcatgtatta
     1441 tgaaaatcca ttttatttat ttcccttgca aaaatccagc cgaagtagaa gcggacaact
     1501 tctccaacat tgatgaaatt tcctcctcag actccttaaa accacccgtg atccactgtt
     1561 ggatcacagc catgcatccg ctcacaatat aaatatgcaa agaagacaaa gcctccgcat
     1621 cgtaatcagg ccggcggctc tgccacattg gcatggtcca gtgttgggtg gcaagcagaa
     1681 gtttattctg aaatcctgga tcaccatggt ctgagaacaa caatcttacc aactgcttat
     1741 gctcactgat ataaaagata atagaattca tcaactgtga aattgaattt tccttctcaa
     1801 actggatcat atcttcttct agagtattta aaatctcatc ttcaatcgac tccagaagat
     1861 ccatcggact ggaataatac gtataaaatg tactgcggtt aatatccgat ttctcacata
     1921 ggcgggaaac ggatattttt tgtattggct gttcttttaa taaataaagc aggctctctc
     1981 tgagcgcacg ttttgttttc aaaatacgac gatcagtttt cttctctacc atatcaaaaa
     2041 ctcctttaaa atcatataaa aaagataatc tacaaaacaa tgaaaaatgt tcgataacca
     2101 ccaaaaacac tattttggtg gttgttgtat acacatacat ttattataat atacatatga
     2161 atgaaaaaca acagttgtat gataaaagga ggcacaaaca tgaaccggca agatttttat
     2221 gatggaaaat tatttgatgc atatcgttat atgggtgcac ataaagacgg tgataagatt
     2281 cgttttgtaa cctatgcacc aagagcctcc aggatatcga tcattgggga gtttaataac
     2341 tggaatgaag aatttatgga acaagacgca caaagtggtt tctttttatt ctactccgac
     2401 aaagctaaag agggtcagat gtacaaatac tgcatctacg gaccagatgg aaaccggcag
     2461 gaacattgtg atccctatgg atttgagatg gaactaagac cgggggcatg ttctattatc
     2521 agagatatca caacatacag attcaatgac agatcatgga tgcagatgag aagtgtcgga
     2581 atggaagatg ccatcaatat ctatgagatg catatgggtt catggagaat gaataagaaa
     2641 gatgagaatg gctggtatca gtatgatgag attgccaaga tgctcgttcc atatctaaaa
     2701 caaaataatt atacccatgt agaattaatg ccactgtcag aacatccatt tgatggatca
     2761 tggggatacc aaaacactgg attttttgca ccaacgaaac gttatggaac tccagataaa
     2821 ttaatgaaat ttgttgatat gatgcatcag gcagggattg gagtcattat ggattttgtg
     2881 ccagttcatt ttgcagtaga tggatatgga ctgaaattat atgatggaac tccattatat
     2941 gaatacgata ataaagatac tggagagagt gaatggggaa gttgcaactt tattcattcc
     3001 agaagagaag ttcagtgctt cttgcaatca gcggccaact attggctgga agagtatcat
     3061 tttgatggaa tccgtatgga tgcagtgagc cgcctgatct actggcaggg agatgaaagc
     3121 agaggagtca acgatacagc gatcgacttc ttaaaagcat tcaatttagg tttgaaacaa
     3181 cgtcatccaa ccgccatgct gatcgcagaa gattccactg cttatccagg tgtgacagaa
     3241 cctgtatgga aaggcgggtt aggatttgat tacaaatggg atttaggatg gatgcacgat
     3301 actctggaat attttcagac tggaccagaa tatcgaagtc gtgattatca caaactaaca
     3361 ttctctannn nnnnnnnggt gtatttctgg aatgaacgtt atatgttaga atattgtcat
     3421 gatgaagttg tacatggcaa ggcaacgatc ttacagaaga tgtatgggga ttatgaagat
     3481 aaatttccac aggcaagggc tatgtatatg tatatgatga tccatccagg aaagaaatta
     3541 aactttatgg ggaatgaatt tggacagatc agggaatgga gcgaagcaca ggaacaggat
     3601 tggatgatct taaaatatcc gattcatgat gccttccata aatatatggt caaattaaat
     3661 gacatttaca agaaagaaaa agctttccat tatgattatc atagagaaaa ctttgaatgg
     3721 ctggactgcc atcaggaaga acgatgtatt tatgcgatcg gaagaaaaac ggcagatcac
     3781 atgatcgttg gaatctttaa tttctctgac aaagagcaga aagattataa attaacgatc
     3841 aaaggacacc atacgagaag aacattgtta catactgaat gggaacagta tggtggaacg
     3901 atgaagaaac gtaaagaaaa tattcatcta aagaaaaaag gaaaagacag tgagcttacg
     3961 atcacacttc caagatacag tggagtttta ttaaaactaa cgaagaaaga ataaggcaga
     4021 aattttgagt aaaaggagat aatatcatga aaatatacga tttagtaatt ataggttccg
     4081 gaccagctgg aatgtctgca gcaatctatg cacagagagc aatgcttgat tttgttatga
     4141 tcgagaaaga atatatgcca ggaggacagg ttgtacagac ttatgaagtt gataattatc
     4201 ctggaattcc aaagacaaat ggaatggatc ttggattaaa attttcagag catgcgaaag
     4261 aacttggtgc taaaagtatt acagcagaag ttaaagaaat ctatgcagac gaagaaatca
     4321 aagagatcat gcttaagaat ggagatacat taaaaacaag gacgatcatt cttgcaacag
     4381 gagcagttca tcagactttg ggaatagaag gagaagaaaa attaaaggga atgggagtat
     4441 cttattgtgc aacatgtgat ggagcatttt ttagaaataa ggtaacagca gttgttggtg
     4501 gtggaaatgt tgcattggaa gatgcaatat tcttaagccg tatgtgtaaa aaagtttatc
     4561 tgatccatcg aagagatgaa tttcgtggtg caaagattct tcaggatgaa gtaaagaaga
     4621 acgacaagat tgaaatctta tggaacacga tcgtgacaaa aatcgatgga gatgaggttg
     4681 tggagggact tcatatacaa gatgtagata accatgtgga taagttacta gatgttgatg
     4741 gaatttttat cgctgtgggg acaaaaccag cttccgagct tttatctgga aagttatcaa
     4801 cagacgaaaa aggatatatt atcgcaggag aagacggtgt tacaaatatt ccgggaatct
     4861 ttgctgcagg ggatggaaga agcaaaaatc ttcgtcaggt ggtgacggcg gtgtcagatg
     4921 gtgccaactg tgtgtattca gtcgagcgat atcttcaaaa tgcctaattt tcagacaatt
     4981 taagctattt ttatatagtg actcataaat taaaaatcaa ccgctttttg tcgatttgtg
     5041 ctagttgtat aacaaaaagc aggttgattt ttttatggaa aaattacaca catattgcta
     5101 acaaaatacc cacaaagtta tcaacataca gaaatggcat attttagcca aaaatgagtt
     5161 acccacagag ttatccacat tatccacatc cacaattcga caaataataa ctgtggataa
     5221 cttcaaaaag aaacatatgt tttgtttact atttataaaa agattttgtt ttcttaatca
     5281 gaattatatg ttataatgaa ttcatacaaa aaaggtaaag gagatgggag ccatgaaatt
     5341 cattattagt ggaaagaaca ttgaagttac agaaggtatc aaagcagcaa ttgaagagaa
     5401 actcggacgt ctcgacaaat atttagtaga cgatacgatc gtgaatgtaa ctttaagtgt
     5461 tcagaaggga agacagaaga tagaagttac cattcctatg aaagggcaca ttatccgtgc
     5521 tgaagaaggc agtgaagaca tgtatgtatc tattgacctg gtagtagatg tgatcgaacg
     5581 ccagatccgc agatataaaa caagacttat gaatcagaaa tatgatggaa caagcttcaa
     5641 acaggaattt atggagcagg aagacgatgt ggaagatgat gagatcagaa tcatccgaag
     5701 caagaagttt gctatcaaac caatggatgt agaagaagct tgtgtacaga tggaattatt
     5761 aggacatgat ttctttgtat tccgcaatgc tgatacattt gaagtcaatg ttgtatacaa
     5821 gagaaaagga aatacatacg gactgatcga acctgaattt tagaagatcg gaagatggag
     5881 gagatcctta tgacaaggat ctcctttttt catacttttt tttataaaaa attcaaaatt
     5941 tattgagaat ttttacaatt agttaacttg caccttataa tgttaagtgt tacaatagca
     6001 aagactatat aatagatata agactatctt gatatcagat gtcagagaca gaacgatcac
     6061 aagatggaac aatcataaaa agaaacttag gagattattt atgggtttat ttgataaagt
     6121 atttggttct cacagtgata aggaactaaa acgaatcagc aagacggttg acaagatcga
     6181 agcactcgat gagtccatgc agaaattgtc agatgaggaa ctaagacata agaccgttga
     6241 atttaaagaa agacttgcaa atggagagac attagatgat ctgttaccag aagcatatgc
     6301 aacagtaaga gaagccagca gccgtgcgat cggtttaaaa cattaccgtg tacagctgat
     6361 cggtggtatc atcctgcatc agggacgtat cgcagagatg agaactggtg aaggtaaaac
     6421 tttagtatcc acgttacctg catatttaaa tgcactggaa gaaaagggcg ttcatatcgt
     6481 aacagtcaat gattatctgg caactcgaga tgccgagtgg atgggaaaag ttcataattt
     6541 tcttggatta actgttggcg ttgttacaaa tgatatggag aatgatgaac gtcgtgaagc
     6601 atataactgt gatattactt atattacaaa caatgaatta ggatttgact acttaagaga
     6661 taacatggtc attgagaaag aagatcttgt acagagagat cttcactatg cgatcgtcga
     6721 tgaggtcgat tccgtactga ttgatgaggc aagaacacca ctgattattt ccggtgaaag
     6781 tggtaagtct acagaattat accgtgcatg tgatatctta gcccgccaga tggaacgagg
     6841 aagttctgat ggtgaattat ccaagatgga tatcttaatg aacgaggata tcgaagaaga
     6901 tggagatttc cttgtcaatg aaaaagataa acagatcatg ctgactgcag atggcgtgaa
     6961 gaaagtcgag aaattcttcc atatcgagaa tcttgcagat ccagagaacc ttgcgatcca
     7021 gcataatatc atcttagcac ttcgtgcaca taacttaatg tttaaagaca aagactatgt
     7081 agtaaaagat gatgaagtat tgatcgtcga tgaatttaca ggacgtatca tgccaggaag
     7141 acgttattct gatggtctgc atcaggcaat cgaagcgaag gaaaatgtaa aggtcaagag
     7201 agaatccaaa actcttgcaa cgatcacgtt ccagaacttc ttcaataaat atgacaagaa
     7261 agcaggtatg acaggtactg cacttacaga agaacaggaa tttagagaga tttatggaat
     7321 ggacgttgtt gtgatcccaa caaacaaacc agttcagaga atcgatcatg atgatgcgat
     7381 ctacaagaca aaacgtgaga agatgaatgc tgttgtcgaa gatatcattg aatctcatgc
     7441 aaaaggacag cctgttttag ttggtacgat caccatcgag atgtctgaag aattaagtgc
     7501 aatgcttaag aaacgtggaa tcaaacacaa cgtactgaat gcgaaattcc acgagaaaga
     7561 agcagagatc atctctcacg caggtgagat cggtgccgta acgatcgcta cgaacatggc
     7621 tggtcgtggt actgacatcg tgctggaaga tggtgttgca gaacttggcg gattaaagat
     7681 catcggtaca gaacgtcatg aatccagacg tatcgataac cagttacgtg gtcgtgctgg
     7741 acgtcaggga gatccaggtg aatctaagtt ctatttatca ctggaagatg atctgatgag
     7801 attgtttgga tctgaaagaa tgatcagtat atacaatgca ctgggaattc cagaaggaga
     7861 agaaatccag cataagacga tcagcaagac aattgaaaaa gcacagaaga agatcgagaa
     7921 taacaacttc ggtattcgtc gtaacctgct cgattatgat caggtaaata atgaccagag
     7981 agaagttatc tacgcagaaa gacgccgtgt attaaatggt gag
//
LOCUS       DAOMUE010000061         7945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2374_length_7945_cov_2.747909, whole genome shotgun sequence.
ACCESSION   DAOMUE010000061 DAOMUE010000000
VERSION     DAOMUE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2374_length_7945_cov_2.747909"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="PLC71_04225"
     CDS             <1..1097
                     /locus_tag="PLC71_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01882.1"
                     /translation="EIKNEKWLKEFYKMMKSDDESSSLLDDSGSYEVWKTSILEDKTG
                     EYELRLRAEDTVLRRFSIQQPDEDIDSDVDFDSYNTDGFYDIIQRPDNTFEENYQKLT
                     DSEKQEIQNLNWNYKDGLLYATNENHKRCINGTSQYYMKNSKLINTMENSGYKGIKKW
                     MEQCEKDTAGIKVKAGDKTYLFHNTDKWHTFKKNGLTCLYNYKRFIIRFDFNKVIGLR
                     SNLSDLPQSGREMWTAISDHSGSAANEVILRSYNDEELYARKNVQIFLQDHKIKQMLI
                     YWDETEAFKGFTKEEKIFLKGCFEKMGISSKDAQEWLNTFMIKKTPQSGKLGSWTYSK
                     GNSKNQVISLINHNENNNYVNFYKKSKGNN"
     gene            1485..3092
                     /locus_tag="PLC71_04230"
     CDS             1485..3092
                     /locus_tag="PLC71_04230"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRM01883.1"
                     /translation="MPVKYVFVTGGVVSGLGKGITAASLGRLLKARGYHVTSQKFDPY
                     INIDPGTMNPIQHGEVFVTDDGAETDLDLGHYERFIDEGLNKKSNVTTGKVYWSILSK
                     ERRGDYGGNTVQVIPHVTNEIKSRFYRSEDPSDQEVAIIEIGGTVGDIESQPFLEALR
                     QFQHEVGHENCILIHVTLIPYLKSSGELKTKPTQASVKDLQGMGIQPDILVCRSDYPL
                     DDGIKRKIAQFCNVERSRVIQNLDAEVLYEVPLMMEKEHLAHEVCECLNMPCPDPDLD
                     DWKKMIDAWKHPEHKVEIALVGKYVSLHDAYISVVESLEHAGVANSADVKIRWVDSER
                     ISSYNVDEMLGGVHGILVPGGFGDRGIEGMICAIKYARENKIPYLGLCLGMQLTLVEF
                     GRHVLGFSDAHSQEFNPDTTHPMVHIMADQDGVTDLGGTLRLGSYPCVLTEGSKAYEL
                     YGEKEIHERHRHRYEVNNEYRDILQENGMMLSGCSPDGRIVEMVEIPEHPWFVATQAH
                     PEFKSRPNKAHPLFKGFVEAALEHQGK"
     gene            3262..5427
                     /locus_tag="PLC71_04235"
     CDS             3262..5427
                     /locus_tag="PLC71_04235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRM01884.1"
                     /translation="MRSNWRKQMMISALALTLSATNVAPVFASAAPDGKTNAADIAQT
                     MGTTAQWNRWEKEWETLKNDWTQISLSPGSNATELNFAWYTPKQTNDDHSNANVPKLI
                     IGEGHNMKNAKVYEAEQTAVKDEQDNNGETYNSNKVTATGLKENKTYYYSYDNGNGYT
                     KPAAYTTKSTNNFSFAFVGDPQIGSSNELKGKDTKEFYDAQSNAVKSDAFNWSSTLNA
                     ALEKTDDQLSFVVSAGDQIQTTKKKLPNKDASKSEIEYTGYLSPEALKSLPVATTVGN
                     HDADNANYTYHFNRTNASELGSNKVVGGDYYFKYGNALFIMLNTQNTNVAEHKQFIEQ
                     TVAANKDCKWRIVTLHQDIYGSAEHSNEPEITNLRYQLTPIFEQNDIDAVLTGHDHAY
                     SRSKMLLGGTKANDYTDNEFDAELEKDMDAGENPTTKTVAPGNIKNDSTDEKDQKYLT
                     YLKSIMDEKAIETVKKQGSSVINPEGVLYMTAGSSSGSKYYDLVPRQQTYIAHRWQED
                     VPTYSVVDVTENSLTINTYRTDNDEKIDETFSITKSKGDTTSLNAEIKASESIVKQKD
                     AYTTESYRVFEQALTGAKEVAADKKATKDEVENALKVLTNAKAGLEKKATTKPATVKK
                     PTAEKKVVVAKASAKLKAGKKKVTVKIKKASVSGYQIKYASNKNFKKAKTRNTRKTIY
                     TIKSLRSKKKCYVKVRAYKVVKGKKIYGSYSKVLKVTVK"
     gene            5430..>6221
                     /locus_tag="PLC71_04240"
     CDS             5430..>6221
                     /locus_tag="PLC71_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRM01885.1"
                     /translation="MKQKQMKGVCLLIAGILFLLLFFSFSGIEKTKLLDTDGRNFEKA
                     EVTNVISGNLSNSGSGKQTVELKLLSGDHKGKIIQATSSQSYLFGAKCKKGMKVIAII
                     NESKGELLASVYSVNRGPMVWLMAAVFVAIVVAVGGRKGISSILSLVFTMLCIFFIFL
                     PMIYKGISPILAAVLVVAFTTVVTMCMVDGITKKSVAAMLGTIIGVVFSGAFALIFGQ
                     VTSITGYNVSDIENLVYVGEMTDIKIGELLFAGILIASLGAVMDVG"
     assembly_gap    6222..6233
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6234..6552
                     /locus_tag="PLC71_04245"
     CDS             <6234..6552
                     /locus_tag="PLC71_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRM01886.1"
                     /translation="SIASTLNELKEQNPQMMLKELFQSGMNVGRDMMGTMTNTLILAF
                     TGGSINTLVFIFAYNYEYQQVINMYSVGIEIMQGLSSSLGVIMAVPVTSWIAAFLYSK
                     NQK"
     gene            6683..7939
                     /locus_tag="PLC71_04250"
     CDS             6683..7939
                     /locus_tag="PLC71_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRM01887.1"
                     /translation="MKVKGVRLYGVKDIRLEEFEIPEIAEDEILLKIECNGIAVSTLK
                     EITLAQRHLRVPKNIKKNPVLIGHEFSGTIAKVGTRWKEDYQEGKQFVLVPEIPHQIE
                     SPGYSYPYFGGAATYCIIPEDVIEKGCLLQYEADSFYELAQAQALYSIVGSFHSNYHS
                     KEGTHDHISGIKEGGNTIILGGAGPMGLMAIRYVLGMKKKPRRLVITDTNQERLEKVR
                     KMIPMQEGTRHGIELYYINPSMFTDSVPVLLAITNEKGYDDVFVYAPPKCVAEIGNRI
                     MAMDGCMNIYASTADKNYRAGMNIYGSHYLKTKLIGSSGGLRSDLIEALDLITTGKIN
                     PAVGITHIGGINAIVDTTLYLNKIPGSKKITYPQINLPLTAIEDFGKLAEKDPVFGEL
                     DESCKRHGGLWNPEAEKILLDHFKKF"
BASE COUNT         3026 a         1210 c         1646 g         2051 t
ORIGIN      
        1 gagaaataaa aaatgagaaa tggctgaaag aattttataa gatgatgaaa tcagatgatg
       61 aaagttcgtc tcttttagat gacagtggaa gttacgaagt atggaaaacg agtatcctgg
      121 aagataaaac gggtgagtat gagctgcgtt taagagcaga agacaccgtt cttcgtagat
      181 tttcaataca gcaaccggat gaagatattg attctgacgt agatttcgac agttacaata
      241 ctgacggatt ttatgatatc attcaaagac cagacaatac attcgaagaa aattatcaaa
      301 agcttacaga ttcagaaaaa caagagattc agaatcttaa ttggaattac aaagacggtc
      361 ttttgtatgc aacaaatgaa aatcataaga gatgtatcaa tggaacatcc cagtattata
      421 tgaaaaactc caaattgatc aatacgatgg agaattcagg atacaaagga atcaaaaaat
      481 ggatggaaca atgcgaaaaa gatacagcag ggattaaagt aaaagctgga gataaaacat
      541 acttgttcca taatacagac aaatggcata cattcaagaa aaatggatta acatgtctgt
      601 ataattacaa acgttttatc attcgatttg attttaacaa agtgattggg cttaggagta
      661 atctatctga ccttccacaa agtggcagag aaatgtggac agctatatcg gatcatagtg
      721 gatcagctgc gaatgaagtt attcttcgtt cttataatga tgaagaatta tatgcaagga
      781 aaaatgttca gatctttttg caagatcaca agatcaaaca gatgttgatc tactgggatg
      841 aaacagaggc attcaaagga ttcacaaaag aagaaaagat attcttaaaa ggctgtttcg
      901 aaaaaatggg tatctcatcc aaggatgcac aagaatggtt aaatacattt atgatcaaga
      961 aaacgccaca gagcggtaag ttaggaagct ggacatacag caaaggaaat agtaaaaatc
     1021 aggtaatatc attgatcaat cataatgaaa ataataatta tgtaaatttt tacaaaaaaa
     1081 gtaaaggaaa taattgacaa atataaaatg atatggtaaa ttatgggtag aaagtgctta
     1141 cagcacaaac tccccttaat aaacagttcg agaactacgg ttttcctgcg atcgttcatc
     1201 gcccataccc catagaatct cgaaagaatt gtattttaga ttatcatcat caacaatcat
     1261 tttatattta tctgcaatcc tctatctaag ttgtgatgtg agcactttcg atttttaaaa
     1321 tatgaaaata aggattccta tgattctatg atatagaaac ataagaatcc ttttcattat
     1381 ttttaacaaa tcacaatgac aaatcaaaaa taatatgata tgatattatt ttgaatggga
     1441 taatataaga aagtagtaga aattcacata taggaggaag aaacatgcca gtaaagtatg
     1501 tatttgttac aggaggagtt gtatctggat taggtaaagg aatcacagca gcttctctgg
     1561 gacgattact aaaagccaga ggctatcatg tcacaagcca gaagtttgat ccgtatatca
     1621 acattgaccc aggaacaatg aacccaatcc agcacggaga agttttcgta acagatgacg
     1681 gagcagagac tgacttagac cttggacatt atgaaagatt tatcgatgaa ggactgaata
     1741 agaaatcaaa cgttacaaca ggtaaagtat attggagtat cttatccaaa gaacgtcgtg
     1801 gagattatgg tggtaacaca gtacaggtaa tcccacacgt aacaaacgag atcaagagcc
     1861 gtttttacag aagtgaagat ccaagcgatc aggaagtagc gatcattgag atcggaggaa
     1921 ctgtaggaga tatcgaaagt cagccattct tagaggcatt aagacagttc cagcatgaag
     1981 taggacatga aaactgtatt ttaattcatg taacattaat tccatactta aagagttcag
     2041 gagaattaaa gacaaaacca acacaggcaa gtgtcaaaga cttacaggga atgggaatcc
     2101 agccagatat ccttgtctgc cgttctgatt atccattaga tgatggaatc aagagaaaga
     2161 tcgcacagtt ctgtaacgtt gaacgttcaa gagttattca gaacttagat gcagaagtat
     2221 tatacgaagt acctttgatg atggagaaag aacatctagc acatgaagta tgtgaatgtc
     2281 tgaacatgcc atgcccagat ccagatcttg atgactggaa gaagatgatc gatgcatgga
     2341 aacatccaga acataaagta gagatcgcat tagttggtaa atatgtatct ttacacgatg
     2401 cttacatcag tgtcgtagaa tccttagagc atgctggagt cgcaaattca gcagatgtga
     2461 agatccgctg ggtagattca gagagaatca gctcttacaa tgtcgatgag atgttaggtg
     2521 gagttcacgg aatcttagtt ccaggtggat tcggagatcg tggaatcgaa ggaatgatct
     2581 gtgcgatcaa atacgcaaga gaaaacaaga tcccatatct tggactatgc ttaggaatgc
     2641 agcttacatt agtagaattc ggaagacatg tcttaggatt ctctgatgca cacagtcagg
     2701 aatttaatcc agacacaaca catccgatgg ttcatatcat ggcagatcag gatggagtca
     2761 cagatcttgg tggaacatta cgtttaggat cttacccatg tgtattaaca gaaggatcca
     2821 aagcatatga attatatgga gagaaagaga ttcacgaaag acatcgtcat agatatgaag
     2881 taaataacga atatcgtgac atccttcagg agaatggaat gatgttatcc ggatgctcac
     2941 cagacggaag aatcgtagag atggtagaga ttccagaaca tccatggttt gtggcaacac
     3001 aggcacatcc tgagtttaaa tcaagaccta ataaagcaca tccattattt aaaggatttg
     3061 tagaagcagc cttagaacat caaggtaaat aacacaaaaa gttaatataa catcatcaaa
     3121 aagattctga gatagattga tatttcagaa tcttttttta catattattt acgattcaat
     3181 gcaattagaa tttacatata tatgatagaa tcctttaaga gtcagaaaga tatgaaaaga
     3241 caaaggtatc aggaggtata tatgagatct aattggagaa aacaaatgat gatcagtgca
     3301 ttagcattga cactttctgc aacaaatgta gcaccagtat ttgcaagtgc cgctccagat
     3361 ggcaagacaa atgcagcaga cattgcacag acaatgggaa caacggcaca gtggaatcgt
     3421 tgggagaaag aatgggaaac tttaaaaaat gactggacac aaatttcatt gtcaccagga
     3481 agcaatgcaa cggagttgaa ttttgcatgg tatacaccaa agcagacaaa tgatgatcat
     3541 tcaaatgcaa atgttccaaa actgatcatt ggtgaaggac ataatatgaa aaatgccaag
     3601 gtgtacgagg cagaacaaac agcagtaaaa gatgaacagg ataacaatgg ggaaacttat
     3661 aattctaata aagtaacagc aacaggatta aaagaaaata aaacatatta ttatagttac
     3721 gacaatggaa atgggtatac aaagccagct gcgtacacga caaaatctac aaacaatttc
     3781 tcatttgcat ttgtaggtga cccacagatc ggatcttcaa atgaattaaa gggaaaagat
     3841 acgaaagaat tttacgatgc acagtcaaat gcagtaaaga gtgatgcatt taactggtct
     3901 tctactttaa atgcagcatt agaaaagaca gatgatcagt taagttttgt tgtatccgca
     3961 ggtgaccaga ttcagacaac aaagaaaaag ttaccaaaca aagatgcatc aaaaagtgaa
     4021 attgagtata caggatattt aagtccagaa gcattaaaat cacttccagt ggcaacgaca
     4081 gtaggtaacc atgatgcaga taatgcaaac tatacatacc attttaacag aacaaatgca
     4141 agtgaacttg gaagtaataa ggtagtcggt ggagattatt atttcaaata tggaaatgca
     4201 ttatttatca tgttaaatac acagaataca aatgtagcag aacataaaca gtttattgag
     4261 cagacagttg cagcgaataa agactgcaaa tggagaatcg taacactgca tcaggatatt
     4321 tatggatcag cagaacattc taatgagcca gagatcacaa atttaagata tcagttaaca
     4381 ccgattttcg aacaaaatga tattgatgct gtattaacag gtcatgatca tgcttattca
     4441 agatctaaaa tgttattagg tggaacaaaa gcgaatgatt atacagataa tgaatttgat
     4501 gcagaattag agaaagatat ggatgcgggt gaaaatccaa cgacaaaaac agtggcacca
     4561 ggaaatatta aaaatgacag cacagatgaa aaagatcaga aatacttaac atatcttaaa
     4621 agtatcatgg atgaaaaagc aattgagacc gtcaaaaaac agggaagctc tgtaatcaat
     4681 ccagaaggtg tcctttatat gacagcaggt tcttcttcag gaagtaaata ttatgatctg
     4741 gtaccaagac agcagacata tattgcacac cgttggcagg aagatgtccc aacatattca
     4801 gttgttgatg taacagaaaa tagtttaacg atcaatacat atcgtacaga taatgatgaa
     4861 aagattgatg aaacattttc tatcacaaag agcaaagggg atacgacatc acttaatgca
     4921 gaaattaaag catctgaaag cattgtaaag caaaaagatg catatacaac ggaaagctat
     4981 agagtctttg aacaggcact tacaggagcc aaagaagttg cagcagacaa aaaagcaaca
     5041 aaagatgaag ttgaaaatgc attaaaagtt ctgacaaatg caaaagcagg attagaaaag
     5101 aaagcaacaa caaaaccagc aacagtaaag aaaccaacag cagagaagaa agttgtagta
     5161 gcaaaagcat ctgcaaaatt aaaagctggc aaaaagaaag taacagttaa gatcaaaaaa
     5221 gcatctgtat ctggatatca gattaaatat gcatcaaaca agaactttaa aaaagcaaag
     5281 acaagaaata caagaaaaac aatttatacg atcaagtcat taagatcaaa gaaaaaatgt
     5341 tatgtaaagg ttcgtgcata caaagtagtt aaaggtaaga aaatctacgg ttcttacagc
     5401 aaagttctga aagtgactgt gaaataacaa tgaaacaaaa acagatgaaa ggagtgtgtc
     5461 tgctgatcgc aggcattctc tttcttctgc tgtttttttc tttttctgga attgagaaga
     5521 caaaacttct agatacagat ggaagaaatt ttgaaaaagc agaagtaaca aacgtgatct
     5581 ctggaaactt atcaaatagt ggaagtggaa aacaaaccgt agaattaaaa cttctctcag
     5641 gggatcataa aggaaaaata atacaggcaa caagctctca aagttattta tttggtgcaa
     5701 aatgtaaaaa aggaatgaaa gtcatagcga tcattaatga atcgaaagga gagttgctgg
     5761 cgtctgtata tagtgtaaac agaggaccta tggtatggct gatggcagca gtgtttgtag
     5821 caatcgtggt tgcagtcggt ggaagaaaag gaatttcatc aatattaagc ttggtattta
     5881 caatgctttg tatctttttt atttttcttc cgatgatcta caaaggaata tccccgatac
     5941 tggcggctgt attagtggtc gcatttacaa cagttgtgac gatgtgtatg gttgatggaa
     6001 ttacgaagaa atcagtagct gcgatgcttg gaacgatcat aggtgtggta ttttctggag
     6061 catttgcatt gatctttgga caagtgacat cgatcaccgg atataatgtg tctgatatag
     6121 agaatctggt ctatgtcgga gaaatgacag acataaaaat tggtgaattg ttatttgcag
     6181 gaatcttgat cgcatcatta ggtgcagtca tggacgttgg annnnnnnnn nnngtcaatc
     6241 gcctctacac tcaatgaatt aaaagaacaa aatccacaga tgatgttaaa agaactgttt
     6301 caatcaggaa tgaatgtcgg aagagatatg atgggaacaa tgaccaatac attgattctt
     6361 gcatttacgg gtggatcgat caacacatta gtatttatct ttgcatacaa ttacgaatat
     6421 caacaggtga tcaatatgta ttccgtaggg attgagatca tgcagggatt atcatcaagt
     6481 ctcggagtta tcatggcagt tccagtgacc tcctggatcg cagcattttt atatagtaaa
     6541 aatcaaaaat aaagaaaaga ctccataaca attcagatct ttatgaattc gcagacagaa
     6601 gtttgagaat atgttataat gaaaaagatt aattaaattt aacaagacat aaatatttga
     6661 ctaagtttag ggggcgaagg ttatgaaagt taaaggagtc agactttatg gagtcaaaga
     6721 tatacgtctg gaagaatttg agatccctga gatagctgaa gatgagattc tcttgaagat
     6781 agaatgtaac ggaattgcag tgtcaacttt aaaagagatc acattggcac agagacattt
     6841 aagagtacca aagaatatta aaaagaatcc tgtactgatc ggacatgaat tttctggaac
     6901 gatagcaaaa gtcggaacaa gatggaaaga ggattatcag gaaggaaaac aatttgtatt
     6961 agtaccagag attcctcacc agatcgaatc accaggatat tcttaccctt attttggagg
     7021 tgcagcaaca tactgtatca taccagaaga tgtaattgag aaaggatgtt tgttgcaata
     7081 tgaagcagat tcattctatg aacttgcaca ggctcaggct ttatatagta tagtgggaag
     7141 ttttcattca aactatcaca gtaaagaggg aacccacgat catatatctg gaattaaaga
     7201 aggtgggaat accatcatcc ttggaggagc cggtcccatg ggactaatgg caatccgtta
     7261 tgtccttgga atgaagaaaa agccaagaag actcgtgatc acagatacga atcaagagcg
     7321 tttggaaaaa gtaaggaaaa tgataccgat gcaggaagga accaggcatg gaatagagct
     7381 ttattatatc aatccatcga tgtttacaga ctccgttcca gtgttactgg caataaccaa
     7441 tgaaaaagga tacgatgatg tgtttgtcta tgcaccaccc aaatgtgtag ctgaaatagg
     7501 gaatcgtatt atggcaatgg atggctgcat gaatatttat gcatcaacag cagataaaaa
     7561 ttatcgtgct ggaatgaata tatacggaag ccattattta aaaactaaac tgatcggttc
     7621 atctggagga cttcgttcag acctgatcga agccttagac ctgatcacaa caggtaagat
     7681 caatccagca gttggaatca cacacattgg aggaatcaat gcgattgtcg atacaacatt
     7741 atatttaaat aagataccag gatcaaagaa gattacatac ccacagatca acctgccatt
     7801 gacagcaata gaggactttg ggaaattagc tgagaaagat ccagtctttg gagaattaga
     7861 tgaatcctgt aaacgacatg gtggattgtg gaatccagaa gcagagaaga tactgcttga
     7921 tcattttaaa aaattttaat ttgta
//
LOCUS       DAOMUE010000062         7934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2376_length_7934_cov_2.715827, whole genome shotgun sequence.
ACCESSION   DAOMUE010000062 DAOMUE010000000
VERSION     DAOMUE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2376_length_7934_cov_2.715827"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PLC71_04255"
     CDS             complement(<1..547)
                     /locus_tag="PLC71_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HRM01888.1"
                     /translation="MSKNYETVIGLEVHVELATKTKIFCGCSTAFGGAPNTHTCPVCT
                     GMPGSLPVLNKQVVEYAMAVGLATNCDITQNCKFDRKNYFYPDNPQNYQISQLYLPIC
                     RNGKVEIDVDGVKKDIRIHEIHMEEDAGKLVHDPWTGDSLVDFNRSGVPLIEIVSEPD
                     MRSADEVIAYLEKLRLIIQYLG"
     gene            complement(548..2020)
                     /gene="gatA"
                     /locus_tag="PLC71_04260"
     CDS             complement(548..2020)
                     /gene="gatA"
                     /locus_tag="PLC71_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRM01889.1"
                     /translation="MSILELTAVELGKKIKDKEITVVEATKAALEQIKAVESDVHGYV
                     SYDEESALKRAEEVQKGIDDGTYTGPLAGVPMAIKDNICTKGYTTTCSSKILENFVPT
                     YSAQAVENLIKEGVVFLGKTNMDEFAMGSTTETSAYGVTKNPWDLEKVPGGSSGGSAA
                     AVAANECFMALGSDTGGSIRQPASYCGVTGIKPTYGTVSRYGLIAYGSSLDQIGPVAK
                     DVTDCATILEAIASHDEKDSTSVRLESYDFTSALKDDVKGMKIGIPKDYFGEGLDEEV
                     KEAVLAAAKTLEEKGAIVEEFDLGLVEYAIPAYYVIAAAEASSNLSRFDGVKYGYRAK
                     DYEGLHNMYKKTRSEGFGAEVKRRIMLGSFVLSSGYYDAYYLKALRTKALIKKEFDKA
                     FEKYDVILGPVAPTTAPKLGDSLSDPIKMYLGDIYTISVNLAGLPGISLPCGKDKNGM
                     PIGLQLIGDCFNEKKIIQAAYTFEQTRAYEAPAIAKGGAK"
     gene            complement(2031..2324)
                     /gene="gatC"
                     /locus_tag="PLC71_04265"
     CDS             complement(2031..2324)
                     /gene="gatC"
                     /locus_tag="PLC71_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRM01890.1"
                     /translation="MANHIDDATIEYVGILAKLELSGEEKEQAKKDMESMLDYVDKLN
                     ELDTSSVEPMSHVFPVKNVFREDVVTNGDGSEDTLKNAPEAQDNAFVVPKTII"
     gene            complement(2441..2854)
                     /locus_tag="PLC71_04270"
     CDS             complement(2441..2854)
                     /locus_tag="PLC71_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01891.1"
                     /translation="MKYEKCPICEEKLRNGVCPMCGYDFNRLDRSRILKDGHWDILTT
                     DKKTSQPKYIHDEKRHPDFKNIKNTKTQSMKTQRKTSTYKNKKEKKRSKKGKWAVIVF
                     ILYLLSFLADLVPEAFTHIEDFINQILNGLNFKKK"
     gene            complement(2991..4376)
                     /locus_tag="PLC71_04275"
     CDS             complement(2991..4376)
                     /locus_tag="PLC71_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HRM01892.1"
                     /translation="MKFLDVEKYTPIQKSHEAYLKELMEYCKDTPRHPSYHIHPPCGL
                     VNDPNGLAYFGGKYHVFYQWFPFGPEHGMKHWAHVISKDLVKWEWSDQMLIPDQEYEK
                     NGCYSGNSIEADGKLYLYYTANYKTEQGKIPKQAMAVMNSDGTILKSPNNPIIDEQPE
                     GLIGEIRDPFVFEKEGAYWMLLGGGSTDGQARLILYKSTDLENWVYQGNIELTGIDLE
                     LGYMYECPSYIEIDGKDVLFLSLMGRTPMGERFHNEFSSVYFIGELNLEDKTFHVESF
                     DEIDKGFDFYAPQVFYGKDRQPMMFAWLGCGAQELPYAKEDMWIHSLTMPRFLTIKEG
                     KLCQEVPENIKNEYAHLDIDSKRIKPEEDTWYINLTDKQISEIQIGDQEDHLSIKIDW
                     AAGHIVADRSTLKHQFSTEYGIQREVSMSEELKNIEIYYDNTFIEIYLNDGKDTMTLR
                     AFPENVEINLI"
     gene            complement(4378..6285)
                     /locus_tag="PLC71_04280"
     CDS             complement(4378..6285)
                     /locus_tag="PLC71_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HRM01893.1"
                     /translation="MDYKKISQDVVDHVGGISNIKGATHCVTRLRLILNDETKYDRKA
                     LENIEGVKGVLFNSGQLMIIFGTGTVNNVYDAFIELTGVKEMSQSDAKAEGMSKMGKL
                     QQGFKVFSDIFIPIIPAFVAAAILTGVKALLTANGLFGLEGSLADQSKLVADIAEFLA
                     IMATTFNYLPILVMYSAVKRFGGNPILGILVGIVMVHPDLVNRNTFVLDPSAASYWHF
                     GPLAIAKVAFQGGVFPAILTAWFMSKVEKIAQKYVPAVVSFVFVPTITIFFANVALFT
                     VFGPVGNMIGNGLAAVIDVLYNSFGAFGAFVFAALLQPLVVTGTHQAIQGIEANLIAT
                     TGFNYIQGIWSVSIIAQGGGAIGMYLLAKKHSKDRDIAMSSFIPTLVGISEPAIFAAN
                     LKYSVIPFVCSCLGAGCGGAFMKLAGVRAIGQGLTGILGLLIVVPNKLVFYVIGNLIA
                     FIVPIVLIVIYNKTKGVPHGEEEEEAESALTIDETAKVPVKAVVDGKVIAIEDVKDGI
                     FSEKVLGDGVGIIPTSETVLAPAEGEICTVMEASKHAIGVRTTNNSVFLIHVGIETVS
                     MNGEGFEYLVKKGQHVKEGQPILKFDKAKIEAKGLNPVVVFVKTDEGNQTPVAFKTGI
                     NVKAGKDVIGE"
     gene            complement(6315..>7264)
                     /locus_tag="PLC71_04285"
     CDS             complement(6315..>7264)
                     /locus_tag="PLC71_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 32"
                     /protein_id="HRM01894.1"
                     /translation="EREEYLLILGARKGEDKRKQTGRLVHFTSKDLKKWEFKGDFWAP
                     GIYTMFEMPEIFKMGDWWYLVFSEYSEGNKIHYRRSKNLYGPWEAPFDDAFDGRAYYA
                     GRTAFDGERRVLFGWVPTRIDNDDKNAYLWGGTFVPHEVFQKEDGTLGVKPVDQMMEA
                     FDGWKDLFNPCMKTIDTKEETLLCEDTGSIAAFKTTVKFEEGTKEFSIRFYKDEETEV
                     SYEYRFFVEENKVVFNKCPNYPWYQCFNIGLERPIKLEADKEYEICLIIDQDISTLYI
                     NGTALNARLCDHPGNGLALTVTDGTLEAKNTKIATKINK"
     assembly_gap    7265..7274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7275..7708)
                     /locus_tag="PLC71_04290"
     CDS             complement(<7275..7708)
                     /locus_tag="PLC71_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 32"
                     /protein_id="HRM01895.1"
                     /translation="MYQLYYQPEGIWVGDIMPYGKEGQFYVYHQRDTRNPGPFGEPFG
                     WALATTKDFVDYKDYGESLKRGTDEEADQFIYAGSVFETEAGFHAFYTGYNREFLKAG
                     KTSQTLLHATSKDGITWEKSKEALEIPPQEGYDKRNWRDPFVL"
BASE COUNT         2042 a         1755 c         1256 g         2871 t
ORIGIN      
        1 cacctaagta ctgaatgatc aggcgtaact tttcaagata tgcgataact tcatctgcag
       61 aacgcatatc tggttctgat acgatttcaa tcaatggcac tccagaacga ttaaagtcta
      121 ctaaagaatc tcctgtccat ggatcatgca ctaatttacc tgcatcttct tccatatgaa
      181 tctcatggat tctgatatct ttcttcaccc catctacatc gatctctact ttaccatttc
      241 gacagattgg taaatataac tgagagatct gatagttctg tggattatct ggatagaaat
      301 agttcttacg gtcgaattta cagttctgtg tgatatcaca gtttgttgca agtccaactg
      361 ccatcgcata ttctaccacc tgtttattca acactggtaa tgatcctggc atacctgtgc
      421 atacaggaca tgtgtgtgta tttggtgctc ctccgaatgc tgtgctgcat ccacagaaaa
      481 tctttgtctt tgttgctaat tctacatgga cttcaagtcc gatgactgtt tcgtaattct
      541 tgctcatcta tttagcccct cctttagcaa ttgctggagc ttcatatgct cttgtctgct
      601 caaatgtata tgctgcctga atgatcttct tctcattgaa gcagtctccg atcagctgaa
      661 gtccgatcgg cattccattt ttgtccttac cacatggaag agagattcct ggaagtcctg
      721 caaggttgac agagatcgta tagatatctc ctaaatacat cttgatcgga tcacttaagc
      781 tgtctcctaa ttttggagct gttgttggtg cgactggtcc taagatcaca tcgtatttct
      841 caaatgcttt gtcaaattct ttcttgatca gtgcttttgt tcttaatgct tttaagtagt
      901 atgcatcgta atatcctgaa cttaatacga atgatcctaa cataatacga cgtttcactt
      961 cggctccaaa tccttcggaa cgagtcttct tgtacatgtt gtgaagtcct tcgtagtctt
     1021 tggctctgta tccgtatttg actccatcaa aacgagataa gttagaactt gcttctgctg
     1081 ctgcgataac ataatatgct ggaattgcat attcaactaa tccaaggtcg aattcttcta
     1141 cgattgctcc cttttcttct aatgtttttg ctgctgctaa aacagcttct ttgacttctt
     1201 catcaagacc ttctccaaag taatcctttg gaataccgat cttcattcct ttgacatcgt
     1261 cttttaatgc tgatgtaaaa tcataagatt ctaatctgac agatgtagaa tctttctcgt
     1321 cgtgagatgc gatcgcttcc agaattgttg cacagtctgt cacatccttt gcaacaggac
     1381 cgatctgatc cagggaagaa ccataagcga tcagtccgta acgggatact gttccatagg
     1441 ttggtttgat ccctgtaaca ccacagtaag atgctggctg tctgatagat ccaccggtat
     1501 cagatcctaa cgccatgaaa cattcgttag ctgcaacagc tgctgctgaa ccacctgatg
     1561 atccacctgg tactttctct aaatcccatg ggttctttgt cacaccatat gcagaagtct
     1621 ctgttgtact tcccatggcg aactcatcca tgtttgtctt tcctaaaaat acaactcctt
     1681 ctttgatcag gttctcaact gcctgtgcgg agtatgttgg tacaaagttc tctaagatct
     1741 tagaactgca tgttgttgta tatccctttg tacagatatt atccttaatt gccattggaa
     1801 ctccagctaa tggtcctgtg taagtcccgt catcgattcc tttctgtact tcttcggctc
     1861 tctttaatgc actttcttca tcgtaagaga catatccatg tacatcactt tcgactgcct
     1921 tgatctgttc aagtgctgcc tttgttgctt ctacaactgt gatctctttg tctttgatct
     1981 tcttgcctaa ttctacggca gttaattcta aaatgctcat cttctgcctc ctatatgatc
     2041 gtctttggca cgacaaaggc gttgtcctgc gcttctggtg cgttctttaa tgtgtcttcg
     2101 cttccgtctc cattcgttac cacgtcttct ctgaatacat tcttcactgg aaatacgtga
     2161 gacattggtt ctacactgct tgtatctagt tcgtttaatt tatctacata gtcgagcatg
     2221 ctttccatat ctttcttggc ctgttctttt tcttcgccag ataattcaag ctttgctaaa
     2281 attccgacat attcaatcgt tgcatcatca atatgatttg ccataagtct tcctcctgtt
     2341 aaattgccta tagagttaat gttaccataa tttttatctt tttaaaatat aaaaatgaac
     2401 aaaagtctgc cgttttttct taaaaatgac agacttttgc ttactttttt ttaaaattta
     2461 atccattcag gatctgattt ataaaatctt ctatgtgagt gaatgcctct ggtacaagat
     2521 ctgctaaaaa tgacaacaga taaagaataa agacaatcac cgcccacttt cctttttttg
     2581 atctcttctt ttctttttta tttttatagg ttgatgtttt cctctgtgtc ttcattgact
     2641 gcgtctttgt attcttgata tttttaaaat ctggatgtcg tttttcatca tgaatatatt
     2701 ttggctgaga agtctttttg tcagtcgtta atatatccca atggccatct tttaatattc
     2761 ttgaccgatc taagcgatta aaatcataac cacacattgg gcagacacca tttcttaact
     2821 tttcttcaca gattggacat ttctcgtatt tcataggccc tgctttctta ctgattcgtt
     2881 tttatcttat ggaaatggta tcatataatc ttagtgtttt caagaattgc tcgactttat
     2941 ctgttatata tcaacaaaaa aatatggcag atttctccac catatctttt ctaaattaaa
     3001 ttaatttcca cattttctgg aaatgcacga agggtcattg tatcttttcc atcatttaga
     3061 taaatttcaa taaatgtatt atcataatat atctcaatgt ttttgagttc ttctgacatg
     3121 gaaacttctc tttgaattcc gtattctgta ctaaactgat gcttaagcgt acttcggtct
     3181 gcaactatat gtcctgctgc ccagtcaatc ttaatactta gatgatcttc ttgatctccg
     3241 atctgaattt ctgagatctg tttatctgtc agattgatat accatgtatc ttcttctggt
     3301 ttaattcttt tactgtctat atcaagatgt gcatattcat tcttaatatt ttctggcact
     3361 tcctggcata actttccttc tttgatcgtt aagaaccttg gcattgtcag actatggatc
     3421 cacatatctt cttttgcata tgggagttcc tgtgccccac acccaagcca cgcaaacatc
     3481 attggttgtc tgtcttttcc atagaatacc tgtggtgcat aaaaatcaaa tcctttgtcg
     3541 atctcatcaa atgattccac atgaaatgtt ttatcttcaa gatttaactc tccgataaaa
     3601 tatacactgg aaaactcatt gtgaaaacgt tctcccattg gtgtacgtcc cattaatgat
     3661 aagaataaaa catcttttcc atctatttca atgtaagatg gacattcata catataacca
     3721 agctccaggt caattcctgt cagttcaata tttccctgat atacccagtt ttcaagatct
     3781 gtactcttat acaaaatcaa tcttgcctgc ccatcggtgc ttccaccacc taaaagcatc
     3841 cagtatgctc cttctttttc aaagacaaat gggtctctga tctccccgat cagtccttct
     3901 ggctgttcat cgatgatcgg attgtttgga gatttaagaa ttgtaccatc actgttcata
     3961 actgccattg cctgttttgg gatctttccc tgttctgtct tatagtttgc tgtgtaataa
     4021 agataaagct taccatctgc ttctatggaa tttcctgaat aacatccatt tttctcatat
     4081 tcctgatctg gaatgagcat ctgatcagac cattcccatt ttacgagatc tttcgagatc
     4141 acatgtgccc agtgtttcat tccgtgttct ggtccaaatg gaaaccactg ataaaacaca
     4201 tggtattttc ctccaaaata cgccagtcca tttggatcgt tcactagtcc acatggggga
     4261 tgaatatgat aggatggatg ccgtggagta tctttacaat actccataag ctcctttaaa
     4321 tatgcttcat ggcttttttg gatcggtgta tatttttcta catctaaaaa tttcatatta
     4381 ttccccgatc acgtcttttc ctgctttgac attgattcct gtcttaaatg ccacaggtgt
     4441 ctggttacct tcatccgttt tgacaaatac gacgactgga tttaatcctt ttgcttcaat
     4501 ctttgctttg tcaaatttga gaattggctg accttccttc acgtgttgtc ctttctttac
     4561 aagatattca aatccttctc cattcatgga tactgtttcg attcctacat ggatcagaaa
     4621 tacactattg tttgttgttc ggacaccgat ggcatgtttt gaagcttcca tcactgtaca
     4681 gatttcacct tctgccggcg ctaagactgt ttcacttgtt gggatgatcc ccaccccgtc
     4741 tcctaatact ttttcagaga agattccatc ttttacatct tcaatcgcaa taacttttcc
     4801 gtcaacaact gctttgactg gtactttcgc tgtctcatca attgttaatg cagattcagc
     4861 ttcttcctct tcttcaccat gtggaacacc ttttgtttta ttataaataa cgatcagtac
     4921 gattggtacg ataaatgcga tcaggtttcc aatgacataa aatactaatt tatttggtac
     4981 tacgatcaga agtcctaata ttcctgttaa tccctgtcca atggctctta cgcctgcaag
     5041 tttcatgaat gctcctccac atccagctcc taagcaagaa catacaaatg gaatcacgga
     5101 atattttaag tttgcagcaa agatggctgg ttctgaaatt cctactaatg ttggaatgaa
     5161 acttgacatt gcgatatctc tatctttaga atgtttcttt gctaacaagt acattccgat
     5221 ggctccacca ccctgtgcaa tgattgatac agaccagatt ccctgaatgt agttaaatcc
     5281 tgtcgtagcg atcagatttg cttcaattcc ctgaatcgcc tgatgtgttc ctgtaactac
     5341 taatggctgt aataacgctg caaagacaaa tgctccaaat gctccgaaac tgttgtataa
     5401 aacatcgatc actgctgcta atccgttacc gatcatattt ccaactggtc cgaatactgt
     5461 aaataatgct acgtttgcaa agaaaatagt aattgttggt acgaatacaa atgataccac
     5521 agctggtaca tatttctgtg cgatcttttc tactttagac ataaaccatg cggttaagat
     5581 tgctgggaat actccaccct ggaatgcaac ttttgcaatt gctaatggtc caaaatgcca
     5641 gtaacttgcg gcagatgggt ctaatacgaa tgtattacgg tttacaagat ctggatgtac
     5701 cataacgatt ccgactaaga taccaaggat cggattccca ccgaatcgtt tgacagcact
     5761 atacattaca aggattggca gataattaaa tgttgttgcc atgatcgcta agaattctgc
     5821 aatatctgca acaagtttgc tctgatctgc taaggatccc tctaatccaa ataatccatt
     5881 ggctgtcaat aatgctttga caccagtcag gatcgctgct gcaacaaatg ctggaatgat
     5941 cggaataaat atatcagaga atactttgaa tccctgctgt aattttccca tcttgctcat
     6001 tccttctgcc ttggcatcag actgagacat ttctttgaca cctgttaatt caataaatgc
     6061 atcatagaca ttgttaactg ttcctgttcc gaaaatgatc atcagctgcc cactattaaa
     6121 taagacacct ttaacacctt cgatattctc taatgcttta cggtcatatt ttgtttcatc
     6181 atttaaaatc agtcgaagtc ttgtcacaca atgtgtcgca cctttgatgt tactgatccc
     6241 accaacgtga tctaccacat cttgtgatat ttttttgtaa tccatctata cttgtctcct
     6301 ttatttctct ctttttattt gtttattttt gttgctattt ttgtgttttt cgcttctagt
     6361 gtaccatctg tcactgtcag tgccaatcca tttcctggat gatcacataa tcttgcattt
     6421 aatgccgttc cattaatata taatgttgaa atatcctggt ctatgatcag acagatttca
     6481 tattctttgt ctgcttccaa tttgatcgga cgctcaagtc caatgttaaa gcactgatac
     6541 catggataat ttggacattt gttaaagaca actttatttt cttccacaaa gaatctgtat
     6601 tcatatgaaa cttctgtttc ttcatctttg taaaatctga tagaaaattc tttcgttcct
     6661 tcttcaaatt ttacagttgt tttgaatgct gcgatgcttc ctgtatcttc acaaagaagt
     6721 gtttcttctt ttgtgtcaat cgttttcata caagggttaa ataaatcttt ccaaccatcg
     6781 aatgcttcca tcatctggtc taccggtttt accccaagtg ttccatcttc tttctggaat
     6841 acttcgtgtg gcacgaaagt tcctccccac aagtatgcgt tcttgtcatc gttatcgatt
     6901 cttgttggca cccatccaaa aagtactctt cgttctccat caaatgctgt acgtcctgca
     6961 tagtatgctc taccgtcaaa tgcatcatca aatggtgctt cccaaggccc gtataggttc
     7021 ttgcttcttc tgtaatggat tttattaccc tcactgtatt ctgagaatac aagataccac
     7081 cagtctccca tcttaaagat ctctggcatc tcaaacattg tgtagattcc tggtgcccag
     7141 aaatctcctt taaattccca tttctttaaa tcttttgatg tgaaatgtac gagacgtcct
     7201 gtctgttttc tcttatcttc tccctttctt gctccaagga tcaacagata ttcttctctt
     7261 tcctnnnnnn nnnnaggaca aatggatctc tccagtttct cttatcatat ccttcctgtg
     7321 gaggaatctc taatgcttct ttcgatttct cccatgtgat cccatctttg cttgtagcgt
     7381 gaagcaatgt ctgtgatgtc tttcctgcct ttaagaattc tctattatat cctgtataaa
     7441 atgcatgaaa tcctgcctct gtttcaaaaa cacttcctgc atagatgaac tgatctgctt
     7501 cttcatccgt tccacgcttt aagctttctc catagtcttt gtaatctaca aaatcttttg
     7561 ttgttgctaa tgcccaaccg aatggttctc caaaaggtcc tggatttctt gtatctctct
     7621 gatgatatac ataaaactga ccttcttttc cataaggcat aatatctcct acccaaatac
     7681 cttctggctg ataatataac tgatacataa tttaatcctt tctttgattt accttgttgc
     7741 tttctatggc tttattatat gtcaaacgtt ttcatatgtc aatcgttacc acatttattt
     7801 ttgcataaaa tattaggaag atttttggta gttttgcaca aaactcattt tattttgtac
     7861 gttctaacat atttttaggc atcaaaaaaa ggaagagtag tattttctct tcctttttta
     7921 ttctccgtta tgtt
//
LOCUS       DAOMUE010000063         7914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2389_length_7914_cov_2.062985, whole genome shotgun sequence.
ACCESSION   DAOMUE010000063 DAOMUE010000000
VERSION     DAOMUE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2389_length_7914_cov_2.062985"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PLC71_04295"
     CDS             <1..248
                     /locus_tag="PLC71_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM01896.1"
                     /translation="SIGGKTATSEKLPRGNGKYISSFLGFAKADNPAIIGIVLIDEPH
                     GTYYGGTIAAPVMRSVFQTALPYMGIYKEYTPDKKEP"
     gene            277..1248
                     /gene="mraY"
                     /locus_tag="PLC71_04300"
     CDS             277..1248
                     /gene="mraY"
                     /locus_tag="PLC71_04300"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRM01897.1"
                     /translation="MNYGIVKPVLIAFFISVLLSPMMIRYLHKLKFGQFIRGEGPESH
                     LKKSGTPTMGGLIILLGIVIASLFYVRRYPMIIPVLFVTLGFGFIGFIDDYIKVVMKR
                     NLGLRAWQKMFGQIIVTGIFAYYLINYTQTGTSMLIPFTHGKYLNLGVFFIPAVFIIL
                     LGTVNGANFTDGLDGLASSVTVLIATFFTVAAIGMGHNDLAAGIWPVSCATVGSLLGF
                     LVFNVYPARVFMGDTGSLALGGFVAATALMLRLSLFIPIVALIYMIEVLSVMAQVLYF
                     KMTKGKRLFKMAPIHHHFELSGWPETKVVAIFAITTAVCCLIGLVAI"
     gene            1262..2626
                     /gene="murD"
                     /locus_tag="PLC71_04305"
     CDS             1262..2626
                     /gene="murD"
                     /locus_tag="PLC71_04305"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRM01898.1"
                     /translation="MIDTSKKFLVAGAGKSGIGSVNLLLKTGADVSLYDGKEDLEKEA
                     LYEKLYEKKEIPLILGNITKEDVSKFDLLVLSPGISVNAPIAQMFRDQEKPVWSEIEL
                     AYQASKGRIAAITGTNGKTTTTALTGEIMKGYYESSFVVGNIGIPYTSVALDTKEDSV
                     TTAEISSFQLETIVDFKPDVCAVLNITPDHLDRHGTVEVYAQTKESIGKNQTKDQVMV
                     LNYEDDTTRAMADRANGTVLFFSLHHKIKEGVSMEGDAFIIEENGKKQIVCTLDDVNL
                     LGDHNYENILAAIAVTYSMGVPVSYIKEGIKRFKGVEHRIEYVDTVKGVAYYNDSKGT
                     NPDAAIKGIKAMNRPTWLIGGGYDKKSTYDEWIEAFDGKVKELILMGVTAPKIADCAK
                     THGFDKIKFVDSMEEAVSYIAGKAQDGEAVLLSPACASWGMFPNYEVRGDIFKDCVRK
                     LKEN"
     gene            2630..3748
                     /locus_tag="PLC71_04310"
     CDS             2630..3748
                     /locus_tag="PLC71_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRM01899.1"
                     /translation="MANRIKEKYMAGKRYFDYPMLFLVIFLICFGLVMIYSTSSYKST
                     VTYGNSYHWLLRQAVAIVLGAVAMVVCCKLDYRIMKSEKFGNGCYWASIVLLVLVLII
                     GAAKKGAVRWISIAGFQFQPSEVSKILVVIYLANRLSANAHKIRTFKDSIVIVLPTVP
                     IIALIVTQNLSTALVVCSMIGVMLFVVSPKMKELMLTAGGGIILLFVYLLTANSYRNE
                     RVQIWLHPESHKKGFQTMQALYAIGSGGIFGKGLGQSMQKMGFIPESHNDMIFSIICE
                     ELGLFGAVCLILVFVALIFRMLLIALNTEDLFGSLIVIGFMTHIAIQVFINIAVVTNT
                     IPPTGIPLPFISYGGTSILVVMIEMGIVLSISKKIHIR"
     gene            3760..4530
                     /locus_tag="PLC71_04315"
     CDS             3760..4530
                     /locus_tag="PLC71_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRM01900.1"
                     /translation="MEEKKVIRMDEHKNKNKVHKLSGKKILLGIVAVLILTVVIIAGT
                     CKTKQIKISGLSYYTEAEVKKAVVENGYIDNSVAYFLKCKIASPDLLPFIDSIHVRIN
                     RPDTITVEVKEKKRAGCLLYNGKYVYFDKNGYALESYEKKYDDVPLVTGLKYDELVMQ
                     EKIPVKKEKVFSYLLELTTAIDKYNLPIDQIYIKEDGNALLISDKITVDLYNKKDIDI
                     KISELAGMLKKVKGKGGTIDMKYFSEDHKIAVFQPKKS"
     gene            4630..5787
                     /gene="ftsZ"
                     /locus_tag="PLC71_04320"
     CDS             4630..5787
                     /gene="ftsZ"
                     /locus_tag="PLC71_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRM01901.1"
                     /translation="MPSVENTQARILVIGVGGAGNNAVNRMVDENVQGVELVGVNTDR
                     QALSLCKAETKIQIGEKLTKGLGAGAKPEIGEAAVEENREEITELVQGSDMVFVTCGM
                     GGGTGTGAAPIIAEISKGLGILTVGVVTKPFTFEGKPRMNNAMSGIARLQDQVDTMIV
                     IPNDKLLQICDKRTTIPDALKKADEVLQQGVQGITDMIYNPGLINVDFADIQTVMRDK
                     GIAHIGMGVADEELEAIKTAMESPLLETTVAGATDVIVNFAGAVGMLEAQQAVEYLKD
                     EAGDDVNVIFGTVNADFGDQISATIIATGIKSADITGNARTGFAAAKKPVQQTQQAPE
                     FSGQPLHNGKVMEEEQPVQTTSYVSEPEMKEIEQESSKINIPEFLLKSRRR"
     gene            complement(5916..6959)
                     /gene="hydE"
                     /locus_tag="PLC71_04325"
     CDS             complement(5916..6959)
                     /gene="hydE"
                     /locus_tag="PLC71_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HRM01902.1"
                     /translation="MNLHELIDRLHKEHTLTKEEFITLIKERNEESASYLASLAREEA
                     VNIYGNGVFPRGLVEFTNYCKNNCYYCGIQGSNPNANRYRLSKEEILSACENGYQLGY
                     RSFVLQGGEDPHYTDEIMVPIVAEIRKRYPDCAITLSLGERSKESYQKLYDAGADRYL
                     LRHEAADLELYQKLHPESLSLENRIQCLWNLKEIGYAVGTGFMVGAPYQTVENLADDL
                     LFIKELDPQMVGIGPFVPHHDTRFKEFPSGSVELTTYLVSILRLMNPHLLLPSTTALG
                     TIDPRGREKGILAGANVVMPNLSPVAVRKDYSLYDNKICTGEEAAECAGCLGRRLASI
                     DYQLVFSRGDYIE"
     assembly_gap    7013..7043
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7139..>7914
                     /locus_tag="PLC71_04330"
     CDS             7139..>7914
                     /locus_tag="PLC71_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM01903.1"
                     /translation="MKREDVRNIAIIAHVDHGKTTLVDEMLKQSGIFRDNQEVAERVM
                     DSNDIEKERGITILSKNTGVMYNGIKINIIDTPGHADFGGEVERVLKMVNGVVLVVDA
                     FEGPMPQTKFVLKKALELDLSVIVCVNKVDRPEARPEEVVDETLELLMELDAGDKQLD
                     CPFVFASAKEGFATLDLDGEKKDMKPLFETIIEHIEAPEGDPEKPLQVLISTIDYNEY
                     VGRIGVGKVENGTISVNQDMVVVNHHDPDKQQRVKIGKLY"
BASE COUNT         2588 a         1278 c         1852 g         2165 t
ORIGIN      
        1 tttccattgg gggaaagacg gcaaccagtg agaaacttcc aagaggaaat ggaaaatata
       61 tttcttcttt tttaggattt gcgaaagcag acaatcctgc gatcattggg atcgtgttga
      121 tcgatgaacc acatggaact tactatggag gaacgatcgc agcccctgtg atgagaagtg
      181 tgtttcaaac tgcacttcca tacatgggga tttataaaga atatacgcca gataaaaaag
      241 aaccgtagac gaaacaagaa caaatgaggt ggcgacatga attatggtat tgtaaaacca
      301 gttttaattg ccttttttat cagcgtactt cttagcccga tgatgatcag gtatttacac
      361 aaattaaaat tcggtcagtt tatccgggga gaaggacccg aatcacatct gaagaaatca
      421 gggactccga cgatgggagg actgatcata ttacttggaa tcgtaatcgc atcgttattt
      481 tatgttagaa gatatccgat gatcatcccg gtgctgtttg tgacgctggg atttggattt
      541 atcggattta tcgatgatta catcaaggta gttatgaaaa ggaacctggg actgcgggcc
      601 tggcagaaga tgtttgggca gatcatagtc acaggaatct ttgcgtatta tttgataaat
      661 tacacgcaga caggaacttc gatgctgatt ccttttacac atggtaaata tttaaacctt
      721 ggagtatttt ttatcccagc ggtttttatc attttgttag gaactgtgaa tggagctaat
      781 tttacggatg gattggatgg acttgcatca agtgttacgg tattgatcgc aacatttttt
      841 acagtggcag ccattggaat ggggcataat gatctggcag ctggaatctg gccggtatct
      901 tgtgcgacgg ttggctcact tcttggtttt ttagtattta atgtatatcc ggcgagagtg
      961 tttatgggag atacaggctc gcttgcactt gggggatttg tggcagcaac agcattgatg
     1021 cttcgcctgt ctttatttat cccaattgtt gcattgatct atatgatcga ggtattatct
     1081 gttatggcac aggtattata ttttaaaatg acaaaaggaa aaagattatt taaaatggca
     1141 ccgatccatc atcattttga attgtcagga tggccagaaa caaaggttgt agcaatcttt
     1201 gcgatcacca cggcagtttg ttgcctgatc ggactggtag cgatttagta aggagaaaga
     1261 aatgatagat acaagcaaga aatttttagt agcaggagca ggaaaaagtg gaattggttc
     1321 tgtcaatctg cttttaaaaa caggtgcaga tgtcagtctg tatgatggaa aggaagacct
     1381 tgagaaagag gcactttatg agaaattata tgaaaagaaa gagattccat tgatcttagg
     1441 aaatatcaca aaagaagatg taagtaagtt cgatctatta gtgttaagtc ctgggatttc
     1501 agtaaatgca ccgatcgcac agatgtttcg tgatcaggaa aagccagtat ggagtgagat
     1561 tgaattagca tatcaggcat ccaaaggaag aatcgcagcg atcacaggaa ccaacggaaa
     1621 gacaacgaca acagcgctga ctggagagat catgaaagga tattatgaaa gcagttttgt
     1681 tgtcggaaat atcggaattc cttatacaag tgttgcgctt gatacaaaag aggacagtgt
     1741 aacaactgca gagatcagca gtttccagtt agaaacgatc gttgatttta aaccggatgt
     1801 atgtgcagtt cttaatatca caccagatca tttggatcgt catggtacag tagaagttta
     1861 tgcacagaca aaagaatcca ttggaaaaaa ccagacaaaa gaccaggtaa tggtattaaa
     1921 ttatgaagat gatacaacaa gagctatggc agacagagcg aatggaacag ttctgttttt
     1981 cagtttgcat cataaaataa aagaaggggt ttccatggaa ggtgatgcat ttatcatcga
     2041 agaaaatgga aagaaacaga tcgtatgtac attagatgat gtgaatctgt taggagacca
     2101 taattatgaa aatattttag cagcgatcgc agtgacttat tctatgggag ttccagtttc
     2161 atatatcaaa gaaggaatca agaggtttaa aggtgtagaa catcgtattg aatatgtgga
     2221 tacagtgaaa ggtgttgcat actataacga ttctaaagga acgaatccag atgcagcgat
     2281 caaaggaatc aaagcaatga accgcccaac atggctgatc ggtggtggat atgacaagaa
     2341 atctacatat gatgagtgga tcgaagcatt tgacggaaaa gttaaagaac taatcctgat
     2401 gggtgtaaca gcaccaaaga tcgctgattg tgcaaaaaca catggatttg ataagatcaa
     2461 gtttgttgat tctatggaag aagcagtttc atacattgca ggaaaagccc aagatggaga
     2521 agcagtttta ttatcacctg catgtgcaag ctggggaatg ttcccgaatt atgaagtcag
     2581 gggagatatc ttcaaagatt gtgtcagaaa attaaaggag aactaaaaag tggcaaatcg
     2641 catcaaagaa aaatatatgg ccggcaaaag atactttgat tatccgatgt tgtttctggt
     2701 aatatttctg atatgttttg gtctggttat gatctatagt acaagttcat ataagagtac
     2761 tgtcacatat ggaaattcct atcactggct gttaaggcag gcagttgcga tagtgttagg
     2821 tgcggttgca atggttgtat gctgtaaact agattaccgc atcatgaaaa gtgagaagtt
     2881 tggaaatggc tgttattggg ctagtatcgt actgttagtt cttgtgttga tcataggtgc
     2941 agcaaagaag ggtgcagtcc gctggatcag catcgcagga tttcagtttc agccatcaga
     3001 agtatctaag atcctggttg tgatctatct cgcgaacaga ttatcagcaa atgctcataa
     3061 gatccgaaca ttcaaagata gtatcgtgat cgttcttcca acggtcccga tcattgctct
     3121 gatcgttaca cagaacttaa gtacagcact tgtcgtgtgt tcgatgatcg gtgtcatgct
     3181 gtttgtggta agtccgaaga tgaaagaact tatgttaaca gcaggcggtg gaataatact
     3241 cttgttcgtg tatctgttga cggcaaatag ttatcgaaac gagcgtgtac agatttggct
     3301 gcatccagaa agtcacaaaa agggatttca gacgatgcag gcattatatg cgatcggttc
     3361 cggtgggatt tttggaaaag gacttgggca gagtatgcag aagatgggat ttattccaga
     3421 atcacacaat gatatgatct tttcgatcat ttgtgaagaa ttaggattgt tcggggcagt
     3481 ttgtctgatc cttgtgtttg tggcactgat ctttcggatg ctcttaattg cactgaatac
     3541 agaagactta tttggatcac tgattgtgat cggttttatg acacatatcg caattcaggt
     3601 attcatcaat atcgccgttg taacaaacac gatcccaccg acaggaattc cacttccatt
     3661 tatcagttat ggaggtacat cgatccttgt tgtcatgatc gaaatgggaa tcgtattatc
     3721 aatttctaag aaaattcaca taagataagg gtgagtgtga tggaagaaaa aaaagtcatc
     3781 cgtatggatg agcataaaaa caagaataaa gtacataaac tatcggggaa gaaaatactg
     3841 cttggtatcg ttgcagtttt gatccttacg gttgtgatca ttgcagggac gtgtaaaaca
     3901 aaacagatca aaatttctgg attaagttat tatacagaag cagaagttaa aaaggcagtt
     3961 gtagagaatg gatatattga taactcggtt gcatatttct taaaatgtaa gattgcgtca
     4021 ccagatcttc tgccatttat agattcgatt catgtaagga tcaaccggcc agatacgatc
     4081 acggtcgagg taaaagaaaa aaagagggct ggatgccttc tgtataatgg aaaatacgtc
     4141 tattttgata aaaatggtta tgcattggaa agttatgaaa agaaatatga cgatgttcca
     4201 ttggttacag ggcttaaata tgatgaattg gtcatgcagg agaagatccc agtcaagaaa
     4261 gagaaagttt tttcttatct tttagaactt acgactgcaa ttgataaata taatcttccg
     4321 atcgatcaga tttatatcaa agaagatgga aatgccttat tgatcagcga taagataacg
     4381 gttgatcttt ataataaaaa ggatattgat atcaagattt ctgaattagc agggatgctt
     4441 aagaaagtaa aagggaaagg tggaactatt gacatgaagt actttagcga ggaccacaaa
     4501 attgctgttt ttcaaccaaa aaagagttga aaaaacttag taaagaaggt atcattaagt
     4561 tagtgtacag aatttaaggg agtcatttta acatagatgg cgtgaaggag gaacaacgtg
     4621 gttgagatta tgcctagtgt tgagaacaca caagcaagga ttttagtaat aggagtcggt
     4681 ggagcaggaa acaatgcggt aaaccgaatg gtagatgaga atgtccaggg tgttgaactc
     4741 gtgggtgtga atacagatag acaggcttta tctttatgta aagcagaaac aaagattcag
     4801 atcggagaga aactgacaaa gggacttgga gcaggagcaa agcctgagat cggggaagcg
     4861 gcagttgaag agaatcgtga agagatcaca gaattggttc agggatcaga tatggtattt
     4921 gtaacatgtg gaatgggtgg aggtaccggt acaggtgcag caccgatcat tgcagagatt
     4981 tccaaaggac ttggaatctt aacagttgga gttgtgacaa aaccatttac atttgaagga
     5041 aagcctcgta tgaacaatgc aatgtcagga attgcacgcc ttcaggatca ggtggataca
     5101 atgatcgtca ttccgaatga caagttgtta cagatctgtg ataagagaac aacgatccca
     5161 gatgcactga agaaagcaga tgaagtactg cagcagggtg tacagggaat tacagatatg
     5221 atctacaacc caggattgat caatgtcgat tttgctgata ttcagacagt tatgagagat
     5281 aaaggtattg cacatatcgg tatgggtgtt gcagacgaag aacttgaagc aatcaagaca
     5341 gcgatggaaa gtccattgct tgagacaact gttgcaggag ctacagatgt gatcgtcaat
     5401 ttcgcaggag cggttggaat gttagaagca cagcaggctg tggaatactt gaaagacgaa
     5461 gcaggagacg atgtcaatgt catcttcggt acagtcaatg cagattttgg agatcagatc
     5521 agtgcaacga tcatcgcaac aggaatcaaa tccgcagata ttacagggaa tgcaagaact
     5581 ggatttgctg cagcgaagaa gccagtacag cagacacagc aggctccgga attttcaggg
     5641 cagccattac ataatggaaa ggtcatggag gaagaacagc cagtacagac aacaagctat
     5701 gtttctgaac ctgagatgaa agagatcgaa caggaaagct ctaagatcaa cataccagag
     5761 ttcttattaa agagcagaag aagataattt cttaacagat aaaagacaga ataaaagcgt
     5821 gtaggatttc aaagattcta cacgcttttt atatcatagg aaattccgat ggaatcccct
     5881 atgcaaggat tctttcttaa ctgctgataa gcaggttatt cgatataatc tccacggctg
     5941 aatacaagct gataatcaat actggcaaga cgtcttccaa gacagccagc acattcagca
     6001 gcttcctctc cagtgcagat cttattgtca tataaagaat aatcttttct tactgcaaca
     6061 ggagaaagat tcggcataac aacatttgca cctgcaagaa ttcctttttc tcttccacga
     6121 ggatcaatcg taccaagtgc tgttgtggaa ggaagcaaaa gatgaggatt catcagacga
     6181 agaatactca caaggtatgt ggtaagttcc acacttccag aagggaattc cttaaatctt
     6241 gtatcgtgat gtggaacaaa aggaccgatt ccgaccatct gtgggtcaag ttctttgata
     6301 aatagcaggt cgtctgcaag attctcaacc gtctgataag gggctccaac cataaatcca
     6361 gttcctacag catagccgat ttcttttaga ttccacagac actggatccg gttttctaaa
     6421 gaaagacttt ctgggtggag tttctggtaa agctctaggt cagcagcttc atgtcgaagc
     6481 agataacgat cagcccctgc atcatataat ttttgataac tttctttgga gcgttcccca
     6541 agagaaagtg tgatggcaca atcagggtag cgctttctga tctctgccac gataggaacc
     6601 ataatttcat ctgtataatg aggatcctca ccgccttgaa gaacaaagct tcgatatcca
     6661 agctggtaac cattctcaca ggcagaaagg atttcctctt ttgacagacg gtaacgattt
     6721 gcattcggat tgctgccttg aattccgcag taatagcagt tatttttgca gtaattcgta
     6781 aattctacta atccacgtgg aaaaacacca tttccataga tatttacagc ttcttctctg
     6841 gcaagagagg caagataaga tgcgctttct tcgttccgtt ctttaataag tgtgataaat
     6901 tcttcttttg tcaatgtatg ctctttatgc aaacgatcaa ttagttcgtg taaattcata
     6961 atgtccaact ccttttctgg tctataagaa atataacata taatagaaga aannnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnttcattg agtgtgatag aataagaaag ttaatcttgg
     7081 catgctttat aaatgccatg gaaactgata cagaaagata ctagaaagaa ggatataaat
     7141 gaagcgcgaa gatgtgagaa atattgcaat cattgcccat gtcgatcatg gtaagacaac
     7201 attagtagac gagatgttaa aacagagcgg aatcttccgt gacaatcagg aagtcgcaga
     7261 gagagtcatg gactcaaatg acatcgagaa agaaagagga atcacgattc tgtctaagaa
     7321 tacaggagtt atgtataatg gaatcaagat caatatcatc gatacaccgg ggcatgctga
     7381 ctttggtgga gaagtagaac gtgtactgaa gatggtaaat ggtgttgtat tagttgttga
     7441 tgcatttgaa ggaccaatgc cacagacaaa gtttgtatta aagaaagcat tagagcttga
     7501 tctgtctgtg atcgtatgtg tcaataaagt agaccgtcca gaagcaagac ctgaggaagt
     7561 tgtcgatgaa acattagaac tattaatgga attagatgca ggggataaac agcttgactg
     7621 tccatttgta tttgcatcag ccaaagaagg attcgcaaca ttagatcttg atggtgagaa
     7681 gaaagatatg aaaccattat ttgagacgat cattgaacat atcgaagcac cagaaggtga
     7741 tccagagaaa ccattacagg tattgatcag tacgatcgac tacaatgaat atgttggtcg
     7801 tatcggagtt ggtaaagtag aaaacggtac aatttccgtg aaccaggata tggttgttgt
     7861 aaaccatcat gatcctgaca aacagcagcg tgtaaaaatt ggtaaactct atga
//
LOCUS       DAOMUE010000064         7790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2442_length_7790_cov_2.904719, whole genome shotgun sequence.
ACCESSION   DAOMUE010000064 DAOMUE010000000
VERSION     DAOMUE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7790
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2442_length_7790_cov_2.904719"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /gene="uvrA"
                     /locus_tag="PLC71_04335"
     CDS             complement(<1..1798)
                     /gene="uvrA"
                     /locus_tag="PLC71_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRM01904.1"
                     /translation="MKKEMIRIKGARENNLKNIDLEVPRNQFVVFTGLSGSGKSSLAF
                     DTIYAEGQRRYMESLSSYARQFLGQMEKPDVDSIEGLSPAISIDQKSTNRNPRSTVGT
                     VTEIYDYFRLLYARVGIPHCPKCGKPISKQTVDQMVDRLMQLEERTRIQLLAPIVRGR
                     KGEHAKVFQNAKKSGYVRVRVDGNVYDLSEDIPMEKNKKHNIEIVVDRLVVKPGIEKR
                     LTDSIETTLNLADGLMMVDVIGGETLNFSQSFSCPDCDISIDEVEPRSFSFNNPFGAC
                     PVCHGLGYKMEFDVDLMIPDQKLSIAEGAIQVFGWQSSTDKKSYTRAILDALAREYHF
                     DLETPFKDYPQEVKDVLLYGTGGREVKVYYKGQRGEGVYDVAFEGIIKNVGRRYREAS
                     QNMKAEYETFMTITPCDECHGQRLKQESLAVTVADKNIAEMCDMSVGEMVSFLETMEL
                     SERQKLIGEQVLKEIKARIGFLNDVGLDYLTLSRATGTLSGGEAQRIRLATQIGSGLV
                     GVAYILDEPSIGLHQRDNDKLIRTLKNLRDLGNTLIVVEHDEDTMLAADYIVDIGPKA
                     GEYGGEVVATGTAKQIMKNKRSITGAYLSGKIK"
     gene            complement(1847..3835)
                     /gene="uvrB"
                     /locus_tag="PLC71_04340"
     CDS             complement(1847..3835)
                     /gene="uvrB"
                     /locus_tag="PLC71_04340"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRM01905.1"
                     /translation="MDHFELVSEYEPTGDQPQAIEKLTKGFQDGNQFETLLGVTGSGK
                     TFTMANIIQNLNKPTLILAHNKTLAAQLYSEFKAFFPHNAVEYFVSYYDYYQPEAYVP
                     STDTYIEKDSSVNDEIDKLRHSATAALIERKDVIVVSSVSCIYGIGSKESYSEMMISL
                     RPGMIKDRDEVIDDLINIQYVRSELDFKRGTFRVKGDVLEILPVSTFEDAVRVEFFGD
                     EIDRIVEFDVLTGEVKRSLNFVAIFPASHYVVPQEQIERAIKGIKEELAEQVTYFKEH
                     DQLIESQRIAERTNFDIEMLRETGFCSGIENYSRHLAGLEPGQPPSTLIDFFGDDFLM
                     IIDESHITVPQIGGMYAGDQSRKSTLVDFGFRLPSAKDNRPLEFGEFEQKIDQMLFVS
                     ATPGKYEEEHELLRAEQIIRPTGLLDPAIDVRPVEGQIDDLLGEIHKETDKGNKVLVT
                     TLTKRMAEELTDYMREVGIRVKYLHSDIDTLERIEIVRDLRMGVFDVLVGINLLREGL
                     DIPEITLVAILDADKEGFLRSETSLVQTIGRAARNSEGHVIMYADHMTDSMHAAITET
                     ERRREIQDEYNKEHGITPQTIKKDIRDLIKISDEHEEETGGYEKDMESMSKKELKEVI
                     ERLSKKMNQAAAELNFELAAQLRDELKEFKIAYQEYDD"
     gene            complement(4088..5698)
                     /locus_tag="PLC71_04345"
     CDS             complement(4088..5698)
                     /locus_tag="PLC71_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01906.1"
                     /translation="MSDEEYKKLLKQYHKLSDRHILVVETDMPYPDVLKVVALSDKIR
                     KAGNELVRLMRKNYDQLMRTKKYRKLLKLYGNTEDKDKRKALANQLNDMQKSYHVTWD
                     FCRTSMIPIGKKYSIDAVFALTKAEDIWRGMEKCLYDNGKTTHFSKYGELPCIRAKQI
                     NRGISMFVEDGKLRFKLRKMQFGIQVNDRFQSDEVNAVLSYLENPDKMDADAVNTLIE
                     EACCIDTYRPCYATLVPRLIRGKYRVYLHLTIEGKAKSKYDRFGNPRHKYGKGMIGAD
                     IGTQTVAYTSDTEVGLKNLSERGNSIQTSERKERLYYRAMDRSRRATNPQNYNPDGTI
                     KKGKKTWKYSDRYKKLKAKHTELCRINAVNRQLAINEDANYLRSLGDTFVTEPKNASK
                     LMKRAKKTTVNSKGKFNKKKRFGKSIKNRCPSGFQTTIEKKFKVTGGAYIEVPNNYRA
                     SQYDHTADDYIKKKLSDRLYRLRDGTLVQRDWYSSFLLYCYDYRTQDIDKDKCITDFG
                     RCYDKEKALIAWIKANHIKILNSGIKVA"
     gene            complement(5691..5858)
                     /locus_tag="PLC71_04350"
     CDS             complement(5691..5858)
                     /locus_tag="PLC71_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM01907.1"
                     /translation="MKSISAIEMFKAYPQLKQFYSRCGVLWSRGYFVSTVGHISEAAV
                     KKYIEEQKDHE"
     gene            complement(5855..6139)
                     /locus_tag="PLC71_04355"
     CDS             complement(5855..6139)
                     /locus_tag="PLC71_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HRM01908.1"
                     /translation="MVDKAEIVKIRKSTISHVSTSLKKLENKGLVEKIQSKDNKKHIE
                     IILLDKAELIIEAGHNAQKQFAKNVLSGLTEEKQVCINIFDKICNNAEEH"
     gene            complement(6568..6963)
                     /locus_tag="PLC71_04360"
     CDS             complement(6568..6963)
                     /locus_tag="PLC71_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRM01909.1"
                     /translation="MLNLPMDHIGLHCSDVELNAKWYQEELGFKVAGKFPGMGGRNVY
                     FLENPQDGKMYEISQRDQLPGGATTRIDHIAYRSSDLEKDYEYCKVNGYKIVSDGIET
                     SLNFWEKGSRCFKIESACGEVIEICQKIK"
     gene            complement(6998..>7790)
                     /locus_tag="PLC71_04365"
     CDS             complement(6998..>7790)
                     /locus_tag="PLC71_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRM01910.1"
                     /translation="EVLDECSRLERKDAVVTYLDPITTGRVEVAMIENELKENTELIS
                     VTAANCQTGTSEPFTGIGTMTSEKGVIFHTDAAFAFGKMPINVERSHIDLLTADASYF
                     GGPENAGFLYVRKSTGAGEYVSETALSEETLTVMSDMAESLAAKATTFMEEIRPVRNH
                     MCERIFAEIEDVELNGHKDHHLTNHLNIRIKNVSAAVVQKALKEEGIEAGIGTNSNIL
                     AAIGRSKAESAESVSFSLKAGTTMEDADKIVDSLKEIVTGERTVL"
BASE COUNT         2029 a         1800 c         1225 g         2736 t
ORIGIN      
        1 tcttaatctt tccgcttaaa taagctcctg tgatagacct cttattcttc atgatctgtt
       61 ttgcagttcc cgttgcaaca acttctccac catattctcc tgctttcggt ccaatatcta
      121 caatataatc tgctgccagc atcgtatctt catcatgctc tacaacgatc agtgtatttc
      181 ctaaatccct tagattctta agtgtacgaa tcagtttgtc attatctctc tggtgaagtc
      241 cgatacttgg ctcatctaag atataagcta ctccaactaa tccagaaccg atctgtgttg
      301 ccagacggat tcgctgcgct tctccaccag ataatgttcc agtagccctt gataacgtca
      361 gataatctag tcctacatca tttaagaatc cgattcttgc cttaatctct tttaaaacct
      421 gttctccgat cagtttctgg cgttcactca attccatcgt ttctaaaaat gaaaccattt
      481 ccccgactga catatcacac atctcggcaa tattcttatc tgcaaccgtt accgccagtg
      541 attcctgctt taatcgctgt ccatgacatt catcgcaagg tgtgatcgtc ataaatgtct
      601 cgtattctgc tttcatattc tgagatgcct cacgatatct tcttccaaca ttcttgatga
      661 tcccttcaaa tgccacatca tagacacctt cgcctctctg ccctttataa tagactttga
      721 cttctctgcc accagttcca tacaataaaa catctttgac ttcctgtgga taatctttaa
      781 atggtgtttc cagatcaaaa tgatattccc ttgcgagcgc atctaagatt gctcttgtat
      841 aactcttctt atccgttgat gattgccatc caaagacctg gatcgcccct tctgcaatac
      901 ttaacttttg gtcagggatc attaaatcca catcaaattc catcttatat ccaagtccat
      961 ggcataccgg acaagctcca aatggattat tgaaggaaaa acttcttggt tctacttcat
     1021 caatactgat atcacagtca gggcaggaaa aactctggct gaaattcaag gtttctccac
     1081 cgatcacatc aaccatcatc aatccatcgg ctagatttaa ggttgtctcg atagaatctg
     1141 ttaatcgttt ttcaatccca ggtttgacca ctaaacgatc caccacgatc tcaatgttat
     1201 gtttcttatt cttctccatt ggaatgtcct ctgacagatc ataaacattg ccatcgactc
     1261 tgacacgaac atatccactt ttcttcgcat tctggaaaac ctttgcatgt tctccttttc
     1321 ttccacgaac gatcggtgcc agaagctgaa tccttgtacg ttcttctaac tgcatcagac
     1381 ggtctaccat ctgatctacg gtctgcttac tgatcggttt tccacacttt ggacaatgtg
     1441 ggattccgac tcttgcgtac agtaatcgaa aataatcata aatctctgtc acagttccta
     1501 ctgtagatct tgggtttctg tttgttgact tctgatcaat agaaatcgca ggagataatc
     1561 cctcaatgct gtctacatcc ggtttctcca tctgtcccag gaactgtctt gcataagaag
     1621 ataaagattc catgtatctt ctctgtcctt ctgcataaat cgtatcaaat gccagagaag
     1681 attttccaga accactcagc cctgtaaata caacaaactg gtttctcggc acttccagat
     1741 ctatattctt taagttattt tctctcgcac ctttgatgcg gatcatctct ttcttcattg
     1801 ataagctcct aattctaatc tatcatcagt tttagtattt cttcttttaa tcgtcatatt
     1861 cctgatatgc gatcttgaat tcttttaatt catctctaag ctgtgcagcc aattcgaagt
     1921 ttaattctgc tgctgcctga ttcatcttct tacttaaccg ctcgatcact tcttttaatt
     1981 ctttcttaga catggattcc atatcttttt catatccacc tgtttcctct tcatgttcat
     2041 cagatatctt gatcagatct ctgatatctt tcttgatcgt ctgtggcgta attccatgct
     2101 ctttgttata ttcatcctga atctcacgtc ttcgttctgt ctccgtgatc gctgcatgca
     2161 tagaatctgt catatgatct gcatacataa taacatgtcc ttcactgtta cgtgctgcac
     2221 gcccgatcgt ctgcacaaga gatgtctcag aacgcaagaa tccctcttta tctgcatcaa
     2281 gaattgcaac caatgtaatc tctggaatat ccaggccttc tcttaacagg ttgattccaa
     2341 ccaatacatc aaaaactccc attctcaaat ctctcacgat ctcaattcgc tctaatgtat
     2401 caatatctga atgtaaatat tttacccgga taccaacttc acgcatataa tcggtcaatt
     2461 cttccgccat tcgcttggtc agtgttgtga caagtacttt attgcctttg tctgtctctt
     2521 tatgaatctc tcctaacaga tcatcaattt gtccttccac cggacgtaca tcgattgctg
     2581 ggtctagtaa acctgttggt ctgatgatct gttctgccct taataattca tgctcttctt
     2641 cgtatttacc aggtgttgca gatacaaata acatctgatc gatcttctgt tcaaattcac
     2701 caaattccaa aggacggtta tctttcgcag aaggcaaacg aaatccaaaa tctactaacg
     2761 ttgactttct ggactgatct cctgcataca ttccaccgat ctgtggtaca gtgatatgcg
     2821 actcatcaat gatcatcaaa aagtcatccc caaagaaatc aattaacgta cttggcggct
     2881 gtcctggttc taatcctgct aaatgtctgg agtagttctc aatcccagaa caaaatcctg
     2941 tttctcttaa catttcaata tcaaaattcg ttcgctctgc aattctctgt gattcgatca
     3001 gctgatcatg ttctttaaaa tatgtcacct gctcagcaag ttcttccttg atccctttga
     3061 tcgcacgttc aatctgttcc tgtggtacaa cataatgtga tgccgggaat attgccacga
     3121 aattcaagct tctctttact tcacctgtta aaacatcaaa ttccacaata cgatcgatct
     3181 catctccaaa aaattcaact ctgactgcat cttcaaatgt agaaaccgga aggatctcca
     3241 acacatcccc tttaactctg aaagtacctc tcttaaagtc aagttcgctt cttacgtact
     3301 gaatattgat caggtcatca atcacctcgt ctctgtcctt tatcatccct ggtcttaaag
     3361 agatcatcat ctctgaatag ctttctttgc taccaattcc ataaatgcag gaaacactag
     3421 atacaacgat cacatctttt ctctcgatca atgctgctgt cgcgctatgt cgtaatttat
     3481 caatctcatc attgaccgat gaatccttct ctatataagt atcggtagat ggaacatagg
     3541 cctctggctg ataataatca taataggaga caaaatattc cacggcgtta tgtggaaaaa
     3601 acgccttaaa ctcactatat aactgtgctg ctaacgtctt attatgtgcc aagatcaatg
     3661 tcggtttatt caaattctgt ataatattcg ccattgtaaa tgtcttacct gaaccagtca
     3721 ctccaagaag cgtctcaaac tgattcccat cttgaaatcc tttggtcagc ttctcgatcg
     3781 cctgtggctg atccccagtt ggctcatatt ctgatactaa ttcaaaatga tccatataac
     3841 ttctccctta tgaaatatct cttatctttt catctatgca tgattctgac tgctcccacg
     3901 ggcaagcccg tggggttctg attgccaagt gtagcttgta atcgtacacc attttcaggc
     3961 tttggagtta caagtgtaaa tctgtttgaa tcaggacttt tattaccaag cagtcctacg
     4021 accacattag cggtaaattt ctgtcttacg acaaggaagt acagtcaatc tccctaacat
     4081 ttagttgtta agctacttta attccactgt ttagtatttt gatatgatta gccttaatcc
     4141 atgcaattag ggctttttct ttgtcatagc acctgccgaa atctgtaata catttatctt
     4201 tgtctatatc ctgcgttctg tagtcgtaac aatagagcaa aaatgatgaa taccaatctc
     4261 tttgtaccaa tgttccgtct cgaagtctgt agagcctatc agacagcttt ttcttaatgt
     4321 agtcatctgc cgtgtggtcg tattggcttg ccctataatt attcggaact tctatatatg
     4381 cgccgcctgt gactttgaat ttcttttcaa tagtagtttg aaatccagac ggacaacgat
     4441 tctttataga tttgccaaag cgctttttct tattaaattt gcctttgcta ttaacagtag
     4501 tcttcttagc ccgtttcatc agcttgcttg catttttagg ttctgtgacg aatgtatctc
     4561 caagacttcg cagatagtta gcatcttcgt ttattgcaag ctgtctattt actgcattta
     4621 tacggcataa ttcagtgtgt ttggctttca gcttcttgta gcgatcagaa tatttccaag
     4681 tctttttacc tttcttgata gtgccgtcag gattgtaatt ctgtggattg gttgcccgtc
     4741 tactcctgtc catagcacga taatataagc gttccttgcg ttcagaagtc tgtatgctgt
     4801 tgccacgctc tgaaaggttc ttcagtccga cttcagtatc agaagtatag gcaactgtct
     4861 gcgttcctat atcagcacct atcatgcctt taccgtattt atgtcttgga ttgccgaatc
     4921 tatcatattt agacttagct ttgccttcta ttgtcagatg cagatatact ctgtacttac
     4981 ctcttatcaa ccttggaaca agagtagcat agcaaggtct ataagtatct atacaacagg
     5041 cttcttctat aagcgtattg acagcatcag catccatctt gtcaggattc tcaagataag
     5101 acaaaacggc gttaacttca tcagactgaa acctgtcgtt gacttgaatc ccgaattgca
     5161 tctttctaag tttgaatcga agtttcccat cctcaacaaa cattgaaatg ccacgattta
     5221 tctgttttgc tctgatgcat ggtaattctc cgtattttga gaaatgtgtg gtcttaccgt
     5281 tatcataaag gcatttttcc atacctcgcc atatatcttc ggcttttgtg agagcaaaca
     5341 ccgcatctat actgtatttc ttgcctatag gaatcataga tgtcctgcaa aaatcccatg
     5401 taacatgata agatttctgc atatcattga gctgattagc gagagcttta cgtttgtcct
     5461 tatcctcagt attgccgtac agttttagta gtttacgata tttcttggta cgcatgagtt
     5521 ggtcgtaatt ctttctcata agacgaacaa gctcgttgcc agctttgcgg attttatcag
     5581 aaagagccac gactttcagg acatcaggat aaggcatatc agtttctaca actaagatat
     5641 gcctgtcaga aagtttatga tattgtttca gaagtttctt gtattcttcg tcactcatga
     5701 tctttctgtt cctcgatgta tttctttact gcagcttcac ttatatgacc tacggtagat
     5761 acaaaatatc ctcttgacca taatactcca caacgagagt agaactgttt taattgtgga
     5821 tatgccttga acatttcaat tgcactgata cttttcaatg ttcttctgca ttattacaga
     5881 tcttgtcaaa tatattgata cagacctgtt tttcttctgt taatccactt aaaacatttt
     5941 ttgcaaactg tttttgtgcg ttatgtcctg cttctatgat cagttctgcc ttatctaaca
     6001 aaataatttc aatatgcttt ttattgtctt tactttgtat tttttcaaca agtcctttgt
     6061 tttccaattt ctttaacgaa gttgaaacat gggatattgt tgattttctg atctttacta
     6121 tttctgcttt gtcaaccatt tatgacatta tacccagtga agtgaactac atcggtaatt
     6181 gaattaccga gcatcagata tatatggctt cgccatacag attctgggtg cgtccatgac
     6241 acctttactg ctaccttttt gaggttacac agctactttt atgatcagga atgaattaaa
     6301 ttttccttca tacacatagt gtaccacatc tccaagaaaa gaacaagtgt tctatttaaa
     6361 aattcaaaaa agaggagacg ttaaagtctc caatgtaacc tgtggttacg cgctttcatc
     6421 tgcggcaatt gaattaccga ggattcccgc tttttatcct aaaaaattta gaaacattcc
     6481 ccaccgggtg cacattaaat aaagaaacgc tagttttaac aactgtctaa actagcgttt
     6541 ctttatttaa aacttgtctt atcgaaacta tttaatcttc tgacaaattt caatcacttc
     6601 tccacaagca ctttcaatct tgaagcagcg agatcctttt tcccagaagt taaggcttgt
     6661 ctcaattccg tctgatacga tcttatatcc gtttactttg caatattcat aatctttttc
     6721 aagatcagat gagcgatatg cgatatggtc gattcttgtt gttgcacctc ctggaagctg
     6781 gtctctctga gagatttcat acatttttcc atcctgagga ttttcaagga aatatacatt
     6841 tcttccgccc attccaggga attttcctgc aactttaaat cctaattctt cctgatacca
     6901 ttttgcgttt aattctacat cagaacaatg aagtccgata tgatccatag gtaaatttaa
     6961 catttgtttg actccttttt taggtgattt tattgtttta taatacagtt ctttctcctg
     7021 tcacgatctc ttttaagctg tcaacgatct tgtctgcgtc ttccatcgtt gttcctgctt
     7081 tcaatgagaa tgaaacggat tctgcacttt cagctttact tcttccgatt gctgcaagaa
     7141 tattagaatt tgttccgatt cctgcttcaa ttccttcttc tttcaaagct ttctgtacaa
     7201 ctgctgcaga tacatttttg attctgatat ttaaatgatt ggtgagatga tgatctttgt
     7261 gtccattcaa ttctacatct tcaatctcag caaaaatacg ttcacacata tgatttctga
     7321 ctggacggat ttcttccata aatgttgttg cctttgctgc taaactttct gccatatctg
     7381 acatcactgt taatgtttct tcgcttaatg cagtttctga cacatattct ccagctcctg
     7441 tgctctttct tacatataaa aatcctgcat tttcaggacc tccaaaataa gatgcatctg
     7501 ctgtcagcag atcaatatga cttctttcta cattgatcgg catttttcca aatgcaaatg
     7561 ctgcatctgt gtggaagatc acacctttct ctgatgtcat tgttccgatt cctgtaaatg
     7621 gctcagaagt tcctgtctgg cagtttgctg ctgtcactga aatcagttct gtgttttctt
     7681 tcagttcatt ttcgatcatt gctacttcca cacggccggt tgtgatcgga tctaaatatg
     7741 tcacaacagc gtcttttcgt tccagtctgg aacattcatc caatacttct
//
LOCUS       DAOMUE010000065         7787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2445_length_7787_cov_2.761252, whole genome shotgun sequence.
ACCESSION   DAOMUE010000065 DAOMUE010000000
VERSION     DAOMUE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2445_length_7787_cov_2.761252"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(431..637)
                     /locus_tag="PLC71_04370"
     CDS             complement(431..637)
                     /locus_tag="PLC71_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HRM01911.1"
                     /translation="MAISKDMIIADLIALDPNYAAILMASGMGCVGCPSSQGESIEQA
                     AYVHGMDLDELLGRLNEYAQTKEA"
     gene            904..1074
                     /locus_tag="PLC71_04375"
     CDS             904..1074
                     /locus_tag="PLC71_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM01912.1"
                     /translation="MAYVISDACISCGACEGTCPAGAISEGDGQYVIDADTCMECGAC
                     ADGCPAGAISQE"
     gene            complement(1121..2101)
                     /locus_tag="PLC71_04380"
     CDS             complement(1121..2101)
                     /locus_tag="PLC71_04380"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRM01913.1"
                     /translation="MKKRPKILVVGSLMMNQGIETSVIPEEGQTVMGEDIKKSPGGKG
                     ANQAVQMARLGADVTLIGKIGRDRDGEELVKVCKEAGVNTEQIIYDDVLPTGSSIVLL
                     ETVPGQKVKKRSIVIPGATTQLTVDEIAYLEDEMDKYDLVVLQNAIPMEVNEAIAGYA
                     YKHEVDVVLNPIPAKKLSKELMECVTYLIVNEQAAKSMTGIKIHSDWKREWTRFNLDE
                     ARVVSAVIRGRGVPTVLITLAEKGVIMNTPERFYYKKAIEDVEMVDPRAAGDAFIGAF
                     CTRICTDDSIGKVLTIANYTAALSIATEGAIESLPTSKQVREYMEQKRDA"
     gene            complement(2200..2733)
                     /locus_tag="PLC71_04385"
     CDS             complement(2200..2733)
                     /locus_tag="PLC71_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM01914.1"
                     /translation="MKEKRRMISEAARQLGLETYHLRTWEEEFSLTIPRNEKGYRCYG
                     DKEIHTFEQIRDMRNAGLDSEEIKQKLSGNIIPFPEEKQMNTSDKMMQFQKVLVKIMG
                     QAILEQKDHIADQISSKVSDRVVKEMDYQFRLREAEEEKRFQKLDEMIRNQQKAREEI
                     AASREKRRFFRRKRKER"
     gene            complement(2847..5114)
                     /locus_tag="PLC71_04390"
     CDS             complement(2847..5114)
                     /locus_tag="PLC71_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HRM01915.1"
                     /translation="MAYLKGYVDHIRFRNEDNGYTVLSLDVDGDEETVVGSFPFLNDG
                     EYISLEGDYVDHPVHGPQFQMRTYEIVAPDDIDSMERYLGSGAIKGVGPALAKRITKK
                     FKMDTFRVIEEEPERLAEVKGISEKKARAIAVEFSEKQEMRQAMMFLSGYGINNNLAV
                     KIYKEYGDHLYTIIQENPYKMTDDIAGVGFKIADEIAKKVGIGSSSDYRIISGIFYTL
                     MRALNEGHVFLPKRILCRNAAHILGVTPEDIEEHLLEMMIDRKIVIEEDEETRVYAAP
                     QYHMEVNTARMLLDLNIHYDVSISEVETMIAMIEETEQITFAEKQKEAIHAVAQDSIV
                     VLTGGPGTGKTTTINGMIQYFEHEGLDIRLAAPTGRAAKRMTEATGYEAMTIHRMLEI
                     NGEADRERDMKKGNASMFERNAGNPLETDVIIIDEMSMVDLYLMNALLQAMVPGTRLV
                     IVGDANQLPSIGAGNVLKDMIASGQFKVVELNQIFRQEEGSHIVRNAHLIHQGRPVEL
                     DNKSRDFFFLQRNNIQDVLGVLVYLVRDKLPGYVHVKPYDIQILTPMRKGELGVERLN
                     QVMQQYLNPPSDEKKEKEIAFGLFREGDKVMQIKNNYQIEWEMRNSKGFTVDKGVGVF
                     NGDMGIITEINDYTEKITVLFDETREVQYPYASLDELELAYAVTIHKSQGSEYPAVVM
                     PILSGPRVLFHRNLLYTGVTRAKNCLTIVGDRNMLFSMIQNVNEQRRYTTLALRLDQI
                     ANESEESGPMDDLGI"
     gene            complement(5203..6207)
                     /gene="mreB"
                     /locus_tag="PLC71_04395"
     CDS             complement(5203..6207)
                     /gene="mreB"
                     /locus_tag="PLC71_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HRM01916.1"
                     /translation="MAAGTDIGIDLGTASVLVYVKGKGVVLKEPSVVAFDRNTNKIKA
                     IGEEARLMLGRTPGNIVAVRPLRQGVISDYTVTEKMLSYFISRTVGKSLFGRKPRISV
                     CVPSGATEVEKKAVEDATYQAGAREVSIIEEPVAAAIGAGIDIAKPCGNMIVDIGGGT
                     ADIAVISLGGVVVSNSIKVADFDEAIVRFMRKKHNLLIGERTAEEIKINVGTVYKRPE
                     NLTMDVRGRNLVTGLPKTVTVTSEETEEALREPAYQIVDAVHNVLERTPPELAADISD
                     RGIVLTGGGSLIQGLEELIEEKTGINTMTAEDPLTAVAIGTGKYIEYLADDDKKSKNK
                     "
     gene            complement(6379..7239)
                     /locus_tag="PLC71_04400"
     CDS             complement(6379..7239)
                     /locus_tag="PLC71_04400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM01917.1"
                     /translation="MWLADQWKDYEVLDTSNGEKLERWGDYFLVRPDPQVLWDTPKKL
                     RQWKKPNGHYHRSHKGGGQWEFFDLPKTWDIQYKELKFHLQPFSFKHTGLFPEQAVNW
                     DWFSDKIRKANRPVKVLNLFAYTGGATLAAAAAGASVTHVDASKGMVNWAKENAQLSG
                     LREKPIRWLVDDCVKFVEREIRRGNHYDGIIMDPPSYGRGPKGEIWKIEEKIYPFIEL
                     CTKILSDDPLFFLVNSYTTGLQPAVLTYMLETQVAAKFGGKVVSDEIGLPVSSNGLVL
                     PCGASGRWEK"
     gene            complement(7418..>7787)
                     /locus_tag="PLC71_04405"
     CDS             complement(7418..>7787)
                     /locus_tag="PLC71_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HRM01918.1"
                     /translation="VIEGGVETKPENILIYATSNRRHLIKETWNDRSDVENSNGMHRS
                     DTMEEKLSLVNRFGVTINYSKPSQKEYFEIVFELARKAGINMGEDELKLEANKWELSH
                     GGISGRTAQQFINHLMGLQA"
BASE COUNT         1950 a         1794 c         1258 g         2785 t
ORIGIN      
        1 ccacgactta tcactctcct tttattattc ttgcagtcag gttgtttgtt acattttgta
       61 agcttcgttc cttacctgtt gattacatta tatctgggta tttttatcaa tacaattctc
      121 tttgggttca aaaaatggag caactattgc tcattcctgt gatgtgcaac atttttgctc
      181 aacgttgcac aaatataaaa attttataaa ctaatcgtaa atttggcata aaatcaaggt
      241 ttttcaatga tattttttgg ttatttttac aattttccaa caacgttttt atgattttaa
      301 tcatgagttt tggtactttt gccactttga tcattattcg caaaaaaaga aaggactata
      361 tctcttagtg cttttaatac actaagagat atagtccttt ttcactttta atttcttatt
      421 gctcgattaa ttatgcttct tttgtctgag catattcgtt taatcttcca agcagttcat
      481 ctaagtccat tccatgaaca taagctgcct gttcaatgct ttctccctga gatgatgggc
      541 atccaacgca tcccattcca gatgccataa ggattgctgc gtagtttgga tctaatgcga
      601 tcagatctgc gatgatcata tcttttgaaa ttgccatttc gtttgttcct ccataaaatt
      661 taataatatt ttcagaaaat atgtttttcg atacatgttt tctaaaatag ttcaaactaa
      721 cattcatagt ataatacatt ctgttcaaag tgtcaaaata actttataaa ttttccactt
      781 attattgtta aaaatacaac aaaattactt gatattcctt cctaagtata ttaaaataga
      841 gatggtcgag cggggtttac ccgctactaa tgattatact atttatataa ggaggatata
      901 actatggcat atgtaatttc agacgcttgt atttcttgtg gagcttgcga aggaacttgc
      961 ccagcaggag ctatcagcga aggagacgga cagtacgtaa tcgatgcaga tacatgcatg
     1021 gaatgtggag cttgcgcaga cggatgtcca gcaggagcta tcagccagga ataatttcca
     1081 ggtacgtata ttgttcaagc aacaaaaaag agatgcattt ttatgcatct cttttttgtt
     1141 ccatatattc tcttacttgt ttcgatgttg gcagagattc tattgcccct tctgttgcaa
     1201 tagataaagc tgctgtataa ttcgctatcg tcagcacttt tccaatagaa tcatccgtac
     1261 agattcttgt acagaatgcc ccgataaatg catctcctgc agctcttgga tcgaccattt
     1321 ctacatcttc gatcgctttc ttatagtaaa aacgttctgg tgtgttcatg atcacaccct
     1381 tttcggctag tgtgatcagt actgttggta ctcctcgtcc acgaatcacc gcagatacga
     1441 ctcttgcttc atcgagatta aatcttgtcc attccctctt ccaatcacta tggatcttaa
     1501 tacctgtcat tgattttgct gcctgctcat tgacgatcag gtatgtaacg cactccataa
     1561 gttcttttga aagcttcttt gccggaatag gattcaatac tacatcaact tcatgtttat
     1621 atgcatagcc cgcaattgct tcattgactt ccatcggtat cgcattctga agcacaacga
     1681 gatcatattt gtccatctca tcttccagat atgcgatctc atcaaccgtt aattgtgttg
     1741 ttgcccctgg tataacgatc gatcttttct ttactttctg tcctggaaca gtctctaata
     1801 atacaatcga agatcctgtt gggagcacat catcataaat gatctgttcc gtgttcactc
     1861 ctgcttcctt acatacttta accagttctt ctccatccct gtctcttccg atcttaccga
     1921 tcagcgtaac atctgcccca aggcgagcca tctgtactgc ctgattagcc cccttgcctc
     1981 cgggtgattt cttaatatct tcacccatca ctgtctgtcc ttcttctggg atcacagatg
     2041 tttctatgcc ttgattcatc ataagacttc cgactaccag gatctttggt cgtttcttca
     2101 tattcgaatt cctcactcta tctattcact cctcatctgt tacagatcag agctgataaa
     2161 tttattataa tattgtctga atccaaatac aatctttact tatctttctt ttcgttttct
     2221 tctgaaaaat cttcgttttt ctctggatgc tgcaatttct tctcttgctt tttgctgatt
     2281 tctgatcatc tcatccagtt tctgaaatct tttttcttct tcagcttctc ttagccgaaa
     2341 ctgataatcc atttctttta ctactctgtc tgaaacctta gaactgatct gatctgcaat
     2401 atgatctttc tgttccagaa tcgcctgtcc catgatcttt accaacactt tttgaaactg
     2461 catcatttta tcagacgtat tcatctgttt ttcttccgga aatggaatga tatttccgga
     2521 taatttctgt ttgatctctt cgctgtctaa tccagcattt ctcatatcac ggatctgttc
     2581 aaatgtatgt atttctttgt ctccataaca acgatatcct ttttcatttc gagggatcgt
     2641 taaggaaaat tcttcttccc atgttcttaa atgatatgtt tcaaggccta actgcctggc
     2701 tgcttctgat atcatacgtc ttttttcttt catatgtccc ttcctttcct ccccttcatt
     2761 ataaggtttg aaaaggactt caaacaagaa aaaccgtcct ctaaaagatg acggtttctg
     2821 gcatttttct ctttattttt tactttttag attccaagat catccatcgg accgctttct
     2881 tcggattcat ttgcgatctg atctaagcgc agtgcaaggg ttgtatacct ccgttgctca
     2941 ttgacattct ggatcatgga aaataacata tttctgtctc ctacgatcgt taaacagttt
     3001 tttgctcttg tgactcctgt atacagaagg tttctgtgga ataatactct tggtccgctt
     3061 aagatcggca tcacaaccgc tgggtattcg cttccctgtg atttgtggat tgtgactgca
     3121 tatgcaagtt ccaattcatc aagacttgca tacggatact ggacttccct cgtctcatca
     3181 aataagactg tgatcttctc tgtgtaatca ttgatttctg tgatgattcc catatcacca
     3241 ttgaatactc caactccctt atctacggtg aatcctttgc tatttcgcat ttcccattca
     3301 atctgataat tgttcttgat ctgcatgact ttgtcacctt cccggaataa tccaaaggcg
     3361 atctcttttt ctttcttctc atcagatggt ggattcaaat attgctgcat gacctgattg
     3421 agcctctcaa caccaagttc accttttcgc attggtgtta agatctggat gtcatatggt
     3481 ttcacatgga catatcctgg tagtttatcc cgtactaaat atactaaaac tcctagtaca
     3541 tcctgaatat tatttctctg taagaagaaa aaatctcggc tcttattatc taattcaact
     3601 ggtcttccct gatggatcag atgggcattt cttacgatat ggctgccttc ttcctgacgg
     3661 aagatctggt ttaattccac gactttaaac tgtcctgatg cgatcatatc ttttaagaca
     3721 tttccagcac cgatcgatgg caactgattg gcatctccta caatgaccaa tcttgttcca
     3781 ggcaccatag cttgcaggag tgcattcatc agatataggt ctaccatgga catctcgtca
     3841 ataatgatca catccgtttc taatggatta cccgcattgc gttcaaacat ggatgcattt
     3901 ccctttttca tgtcacgttc cctgtcagct tccccattga tctctaacat acgatgaatc
     3961 gtcattgctt catatcccgt tgcctctgtc atacgctttg ctgctcttcc tgttggtgct
     4021 gccaaacgga tatccaatcc ttcatgttca aaatactgga tcattccatt gatcgttgtc
     4081 gtcttaccag ttccaggacc tccggttaag acaacgatac tatcctgtgc cactgcatgg
     4141 atcgcttctt tctgtttttc agcaaatgta atctgctctg tctcttcgat cattgcgatc
     4201 attgtctcca cctcagaaat ggaaacatca taatggatat ttaaatccag cagcattctt
     4261 gccgtattga cttccatatg atactgtggt gctgcataaa ctcttgtctc ttcatcctct
     4321 tcaatgacga tcttgcgatc gatcatcatt tctaataaat gctcttcgat atcttctggt
     4381 gttaccccta agatatgagc tgcatttctg cacaaaatac gttttggcaa aaatacatgg
     4441 ccttcattta gtgcccgcat taaggtgtaa aaaattccag aaatgatacg gtaatctgag
     4501 ctgcttccaa tcccgacttt cttcgcgatc tcatctgcga tcttaaatcc gactcctgca
     4561 atatcatccg tcatcttata tggattttct tgaatgatcg tatacaaatg atccccatat
     4621 tctttataaa tcttcacagc aagattatta ttgattccat atccagacag aaacatcatc
     4681 gcctgacgca tttcctgctt ctcactgaat tccacggcga tcgctctggc tttcttctcg
     4741 ctgattcctt ttacttctgc aagacgttct ggctcctctt cgataactcg gaaggtatcc
     4801 atcttaaact tctttgtaat acgttttgca agtgcaggtc cgactccttt gatcgctccg
     4861 gaacctaaat agcgttccat gctgtcaata tcatctggag ccacaatctc ataagttctc
     4921 atctgaaatt gtggtccatg cactgggtga tcgacataat ctccttccaa agatatgtat
     4981 tctccatcat ttaagaaagg aaatgatcca acgacagtct cttcatctcc atcgacatca
     5041 aggcttaata cagtatatcc gttatcttca ttccgaaaac gtatatgatc tacgtatcct
     5101 ttcaaatatg ccattgtttt ctctcctttc ttctgatcta cagtataacg tgaattaagg
     5161 ccaggatgaa ttgccatcct ggcctctttg aatctctaat gattatttgt tctttgattt
     5221 tttatcatca tctgctaaat attcgatata ttttcctgta ccgattgcta cagctgttaa
     5281 aggatcttct gctgtcattg tgttgattcc agtcttctct tcgatcagtt cttctaatcc
     5341 ctgaattaaa gatccaccac ctgttaaaac gattccacga tcagaaatat cagctgctaa
     5401 ttctggtgga gttctctcca gtacattgtg tactgcgtct acgatctggt atgcaggttc
     5461 tcttaaagct tcttctgttt cttcagatgt cacagtcact gtctttggaa gtcctgttac
     5521 aaggttacgt ccgcgaacgt ccattgtaag attttctggg cgtttgtata ctgtacctac
     5581 attgatcttg atctcttctg ctgtacgttc tccaattaat aagttatgtt tcttacgcat
     5641 aaaacgtacg attgcttcgt caaagtcagc tactttgata gagttgctta ctacgactcc
     5701 acctaaagag ataactgcaa tgtcagctgt accaccaccg atatcaacga tcatatttcc
     5761 acaaggtttt gcaatgtcga ttcctgcacc gatcgctgct gctactggct cttcgatgat
     5821 ggaaacttct cttgctcctg cctgatatgt tgcatcttct acggctttct tctctacttc
     5881 agttgcacca cttggtacac aaacactgat gcgaggtttt cttccaaata aagacttacc
     5941 aactgttctg ctgataaaat aacttaacat cttctctgta actgtataat cagagataac
     6001 tccctgacgt aatggacgaa ctgccacgat atttcctggt gttcttccta acattaaacg
     6061 tgcttcttct ccgatcgctt tgatcttgtt tgtatttcta tcaaatgcca ctacagatgg
     6121 ctcttttaat actacacctt ttcctttgac gtaaactaat acgctggcgg ttcctaaatc
     6181 aattccaata tctgttcctg ctgccatagc aaaaattccc ctttcgcttt ctcttatcta
     6241 tcattattat gttatttcta ctttatccgt atacgtgcag ttctacacac gtacacatac
     6301 tctcttatta taaacatttt ccaagaaaat tcaacgattt tttacatatt ctgcgaaatt
     6361 tcagtaaatc ttaagaactt acttttccca gcgtccagat gccccacatg gaaggaccaa
     6421 tccgttggat gaaactggta atccgatctc atctgagact acttttccac caaatttcgc
     6481 tgctacctga gtctctaaca tataagttaa aactgctggc tgtaaacctg ttgtatagga
     6541 gtttacaagg aagaataatg ggtcatcgga cagaatcttt gtacatagtt caatgaatgg
     6601 ataaatcttc tcctcgattt tccagatttc tccctttggt cctcttccgt atgatggagg
     6661 gtccatgatg attccatcgt aatgatttcc tcttcggatc tcacgttcta caaacttcac
     6721 acagtcatct actaaccatc gaattggttt ctctcttaat ccagaaagct gtgcattctc
     6781 ctttgcccag tttaccatac ctttggatgc gtcaacgtgt gtgactgatg ctcctgctgc
     6841 tgccgctgca agtgttgccc ctcctgtgta ggcaaataag tttaatactt ttactggacg
     6901 atttgctttt ctgattttat ctgaaaacca gtcccagttc actgcctgtt ctgggaataa
     6961 tcctgtatgt ttgaagctga atggctgtaa gtggaatttt aattctttgt actgaatatc
     7021 ccatgtctta ggaagatcaa agaattccca ctgtccgccg cctttgtgac ttctgtgata
     7081 atgtccattt ggcttcttcc actgtcttaa cttctttggg gtgtcccata gaacctgtgg
     7141 gtctggcctt accagaaaat aatctcccca gcgttctaat ttctcaccgt ttgatgtatc
     7201 taagacttca tagtctttcc attggtctgc taaccacata ttgtatattc tcactttctg
     7261 tttcttctat aaaatatcat cttgtgcatg tatctacata cttatgttta ttatagccta
     7321 aagacaaatt ctttcgcaag ggttccatat agaaaaaaag aactttctgc atcttttcca
     7381 tgcagaaagt tcttaaacta tgtaattcct atgattttta agcctgaagt cccatcaaat
     7441 gattgataaa ctgctgtgcc gtacgtccag agattcctcc gtggcttaat tcccatttgt
     7501 ttgcttcaag tttcagttca tcttcaccca tattgattcc tgctttgcgg gcaagttcga
     7561 aaacgatctc gaagtattct ttctggcttg gttttgagta gttaattgta acaccaaaac
     7621 ggtttaccag agataatttt tcttccattg tatcggatct gtgcattccg ttactgtttt
     7681 ccacatcaga tctgtcattc catgtctctt tgatcagatg acgacggttg gaggttgcat
     7741 agatcaagat gttctcaggt tttgtttcca ctccaccttc gatgact
//
LOCUS       DAOMUE010000066         7719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2478_length_7719_cov_2.472991, whole genome shotgun sequence.
ACCESSION   DAOMUE010000066 DAOMUE010000000
VERSION     DAOMUE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2478_length_7719_cov_2.472991"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLC71_04410"
     CDS             <1..353
                     /locus_tag="PLC71_04410"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRM01919.1"
                     /translation="AAKELIEAAQAVEKAGAFMLVLEGIPEKLAAIITKKVHIPTIGI
                     GASAECDGQVLVYQDMLAMYGNFVPKFVKQFAQVGEVMQGAVKDYINAVKEGTFPGPE
                     NTYAISDDVIEKLY"
     gene            432..1274
                     /gene="panC"
                     /locus_tag="PLC71_04415"
     CDS             432..1274
                     /gene="panC"
                     /locus_tag="PLC71_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRM01920.1"
                     /translation="MKIVGTVKEVREQVKEWKKQGLSVGFVPTMGYLHEGHKSLMDAA
                     RKGNDKVVVSIFVNPMQFGPTEDLATYPRDLDHDAALCESAGVDLIFHPEAEEMYEKD
                     FCSFVDMTGLTEGLCGKTRPIHFRGVCTVVNKLFNIVTPDHAYFGQKDGQQLAVIKRM
                     VRDLNMDIEIVGCPIVREEDGLAKSSRNTYLSPEERKAALILSKTVALGKELAKTEKD
                     ANKVVEAMKKNIETEPLAKIDYVEAVDALSMAPVEKLEGACMLAMAVYIGKTRLIDNT
                     LINE"
     gene            1293..1682
                     /locus_tag="PLC71_04420"
     CDS             1293..1682
                     /locus_tag="PLC71_04420"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRM01921.1"
                     /translation="MNISMLKGKIHRATVKQAELDYVGSITVDPVLLEAAGILEYEHV
                     HIVDVNNGSRFETYTIPGEPGSGMICLNGPAARCVATGDKIIIIAYADMTPEEAKTFE
                     PKVVFVDDENQISRCTNYERHGRLEDM"
     gene            1693..2874
                     /gene="coaBC"
                     /locus_tag="PLC71_04425"
     CDS             1693..2874
                     /gene="coaBC"
                     /locus_tag="PLC71_04425"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HRM01922.1"
                     /translation="MLKGKTIVLGVTGSIAAYKMANVASMLVKRGCEVHVVMTKNATN
                     FINPIAFESLTNTKCLVETFDRNFQFHVAHVSLTDKADAMLIAPASANIIGKIANGIA
                     DDMLSTTVMACNKPVIISPAMNTKMYNNPILQDNLDKLRRFGYEIVEPANGHLACGTS
                     GAGKMPSEEVLVAHLERVIAKEKDLKGKKVLVTAGPTIEAIDPVRYISNHSSGKMGYA
                     IAKMAMLRGADVTLVTGKTAIEPPMFVDVVPIVSAQDMYDAVISRSDEQDIIIKSAAV
                     ADYTPANVSDQKVKKKDGDMAIELVRTKDILKTLGENKKEGQFLCGFSMETQNMLENS
                     RAKLEKKKIDMIAANNLKEQGAGFNTDTNVITKDEEKQLPKMTKEEVADALLDFIVSK
                     N"
     gene            2957..4444
                     /locus_tag="PLC71_04430"
     CDS             2957..4444
                     /locus_tag="PLC71_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM01923.1"
                     /translation="MKAVNKVRLIYLPAIVLPIFVMIVSIAGLSIGYSRINKADLSAG
                     SFERFVNPLKTMNTETQEIFFELEDHVKKDPQIFDNEQYLNDLNERLKAKDSYLIVRK
                     NGQIQYIGKKKVSDKLIHKLPSYGNKDSDADRGFFVSRPGNYLVKQQDFKYNDGGQGS
                     VFIMTDLGTVLPHYRNIIIQVSCAVIGVLILTSTFLSWFMYREFVGPIRELKAGAERI
                     KEGNLDNDVVINSGGEEIRELCNAFNEMRAELKDSIDARIVYEKQNRDLISNISHDLK
                     TPITAIKGYVEGIMDGVADTPEKMDRYIKTVYNKANDMNVLINELSLYSKIDSNIIPY
                     NFEKININSYFKDCVDEIGADLEQRGMMFSYRNYCAANVMVTADPEQLKRVINNIINN
                     ACKYNNKAKGKVGITLTEYDRKVQVAIKDNGIGISKEDLPHIFRRTYRADMSRNSAGG
                     SGLGLSICKKIIEEHGGEIWAESKLRAGTTIFFTLNKVIDQSKEK"
     gene            4446..5135
                     /locus_tag="PLC71_04435"
     CDS             4446..5135
                     /locus_tag="PLC71_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM01924.1"
                     /translation="MSKVLIVEDELSIAELEKDYLELSSFEVEIETDGQQGLEKALKN
                     DFDMIILDIMLPGVDGFEICKQIREAKEIPILMVSARKEDIDKIRGLGLGADDYITKP
                     FSPSELVARVKAHLNRYQRLVNNQMEVNDIIEIRGLKIDKTARRVYLNGEEKVFTTKE
                     FDLLTFLAGHPNRVFTKEELFNKIWDMESLGDIATVTVHIKKIREKIEVNTAKPQYIE
                     TIWGVGYRFKM"
     gene            5145..5855
                     /locus_tag="PLC71_04440"
     CDS             5145..5855
                     /locus_tag="PLC71_04440"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM01925.1"
                     /translation="MRKNKKINIIYKDDQIVVCEKPKGIPVSSDKTGDMDLLHQLKNQ
                     LFFEENTDGEPQLYLVHRLDRPVGGVMVFARTKKAAASLTEQIKDHTFEKDYQAVLTG
                     WLPDDEGTFRDYLLKDPKNNVSKIVKEGTPGAKEAILDYEVIDMMEDSKMKYTYCLIH
                     LETGRHHQIRVQTSSRGFGIWGDTKYNPIYQKTKKKYKEIGLFAARIAFEHPTTGERM
                     VFKADPSGEAFDLLEMTE"
     gene            5876..6670
                     /gene="hisA"
                     /locus_tag="PLC71_04445"
     CDS             5876..6670
                     /gene="hisA"
                     /locus_tag="PLC71_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HRM01926.1"
                     /translation="MRFRPCIDIHNGKIKQIVGGSLLDQGDFATENFVSEKDGTFYGK
                     LYKENHLNGGHIIMLNARDSKYYEEDKVQALKALKAFEGGLQIGGGITDENAKEFIES
                     GASHVIVTSFVFAGGKVHYDRLDALKEQVGREHLVLDLSCRKRDDKYYITTDRWQKFT
                     EEELTIELLHKLESWCDEYLIHGVDVEGKAQGIDQELVQLLARYNGNKVTYAGGVKNF
                     DDLELLKNYGNDKIDVTIGSALDLFGGDMEFSKVLEYCDATITNLN"
     gene            6721..7194
                     /locus_tag="PLC71_04450"
     CDS             6721..7194
                     /locus_tag="PLC71_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01927.1"
                     /translation="MKLLYEASNFTIQARWVIPFAILGIIGVLLLLCSRKPFFKLLAA
                     VCLVICAIAALRFCFGYVQVIQAYKQGNYKSVEGYVENFVPASSGGEDQEDMEEESFD
                     IKGVHFSYGDKFIKKFGYHRPSTQGGYIKKDGQYLKIGYISTEKGYVIVKIQERK"
     gene            complement(7285..>7719)
                     /locus_tag="PLC71_04455"
     CDS             complement(7285..>7719)
                     /locus_tag="PLC71_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01928.1"
                     /translation="IAVIAAIILGVLYFVGRKMQAKQEDQRAQLDQMAQTVNMLVIDK
                     KRMKIMEAGFPKMVTDNIPKRAKMSKVPVVKAKIGPKITPLLCDEAIFDDVPVKAEIK
                     AVVSGIYITSIKNLRNVAPPEPEKRGLRAKLTKKSAEFMRKN"
BASE COUNT         2762 a         1212 c         1749 g         1996 t
ORIGIN      
        1 aggcagcaaa agaactgatc gaagctgcac aggcagttga gaaagcggga gcatttatgc
       61 tcgttttaga aggaattcct gagaaattag cagcgatcat cacaaagaaa gtacatattc
      121 caacgatcgg aatcggagca agtgctgaat gtgatggaca ggtattagtt tatcaggaca
      181 tgttagcaat gtatggaaac ttcgtaccaa aatttgtaaa acagtttgca caagttggag
      241 aagtgatgca gggagcagtc aaagattata tcaatgcagt caaagaagga actttccctg
      301 gaccagaaaa tacatatgcg atcagtgatg atgtcattga aaaattatat taagatcaaa
      361 aacagacaat aaaaaggaca taatttatat aaatagattc ccacgtataa tgggaagaag
      421 gagttttagt aatgaaaatc gtaggaacag tcaaagaagt cagagaacag gtaaaagagt
      481 ggaagaaaca aggactaagc gtaggtttcg tgccaacgat gggatatctt catgaaggac
      541 acaaaagtct gatggatgca gcaagaaaag gtaacgataa agtcgttgta agtattttcg
      601 taaatccaat gcagtttgga ccaacagagg accttgcaac atatccaaga gatttagacc
      661 atgatgcagc attatgtgaa agtgcaggag tagacctgat cttccatcca gaagcagaag
      721 aaatgtatga aaaagatttc tgttcttttg tggatatgac aggacttaca gaaggacttt
      781 gtggaaaaac tcgtccaatt catttcagag gcgtatgtac ggttgtaaat aaattattta
      841 atatcgtaac accagaccac gcatattttg gacagaaaga cggacagcag ttagcagtga
      901 tcaaacgtat ggtacgtgac ttaaatatgg atatcgagat cgttggatgt ccgatcgttc
      961 gtgaagaaga cggacttgca aagagttcaa gaaatacata tctttctcct gaagaaagaa
     1021 aagcagcact gatcctttct aagacggtag cattaggaaa agaacttgcc aagacagaaa
     1081 aagatgcaaa caaagtcgta gaagcaatga agaaaaacat tgagacagaa ccacttgcca
     1141 agattgatta tgtagaagca gttgatgctt tatctatggc accagttgag aaactggaag
     1201 gagcatgtat gttagctatg gcagtttaca ttggaaagac aagactcatt gacaatacac
     1261 tgattaacga atagacttaa ggagaaagaa aaatgaacat cagcatgtta aaaggaaaaa
     1321 ttcatagagc aacagtaaaa caggcagaat tagattatgt aggaagtatc acagtcgatc
     1381 ctgtattatt agaagcagca ggaatcttag aatatgaaca cgtacatatc gtagatgtga
     1441 acaacggaag ccgtttcgag acatatacaa ttccaggaga gccaggaagt gggatgatct
     1501 gcttaaacgg acctgcagca cgttgcgtgg caacaggaga taagatcatc attatcgcat
     1561 atgcagatat gacaccagaa gaagcaaaaa catttgaacc aaaagttgta tttgtagatg
     1621 atgagaatca gatttcccgt tgcacaaact atgaaagaca tggacgttta gaagatatgt
     1681 aggagaaaga tcatgttaaa aggaaaaacg atagtattgg gtgtgacagg aagtattgca
     1741 gcttataaga tggcaaatgt agcgagtatg ttagtgaaac gtggatgcga agttcacgta
     1801 gtcatgacga agaatgcaac aaatttcatc aacccgatcg catttgaaag tttgaccaat
     1861 acaaaatgtc ttgtagaaac atttgaccgt aattttcagt ttcatgtagc acatgtatca
     1921 ctgacagaca aagcagatgc gatgctgatc gcaccagctt cagcgaatat cattggtaag
     1981 atcgcaaatg gaatcgcaga tgatatgtta tcaacaaccg taatggcatg taacaaacca
     2041 gtgatcatct caccagcgat gaatacaaag atgtataata atccgatctt gcaggataat
     2101 cttgataaac taagaagatt tggatatgag atcgtagagc ctgcgaatgg acatctggca
     2161 tgtggaacaa gtggtgcagg aaagatgcct tcagaagaag tgctggttgc acatcttgag
     2221 agagtgatcg ccaaagaaaa agacttaaaa ggcaagaaag tccttgtgac agcagggcca
     2281 acgatcgaag caatcgatcc agtacgttat atttccaatc attcctcagg aaagatggga
     2341 tatgcgatcg ctaagatggc gatgcttcgt ggagcggatg ttacgttagt cacaggaaag
     2401 acagcgatcg aacccccgat gtttgtagat gttgttccga tcgtgagtgc acaggatatg
     2461 tacgatgcag tgataagcag aagcgatgaa caagatatca tcatcaaatc tgcagctgtt
     2521 gcagattata caccagcgaa tgtcagcgat cagaaagtca agaaaaaaga cggagatatg
     2581 gcgattgaac tggtaaggac aaaagatatt ttaaagacac ttggagagaa taaaaaagaa
     2641 ggtcagttct tatgtggttt ttccatggaa acacagaaca tgttagaaaa ctccagagct
     2701 aaactagaga agaaaaagat cgacatgatc gcagccaata acttaaagga acagggagct
     2761 ggattcaaca cagatacaaa cgtgatcaca aaggatgaag aaaagcagct tccaaagatg
     2821 acaaaagaag aagttgcaga tgcacttctt gattttatcg tttctaaaaa ttaaataaaa
     2881 taatcgatca aattctggtt tcttcgtatg actatggtat aataatagat attaatacca
     2941 aaggagagtt aatgggatga aagcagtaaa taaagttcgg ttgatctatt taccagcgat
     3001 cgtgctacca atctttgtga tgatagtatc gatcgctggt ttgagtatag ggtattcaag
     3061 aatcaacaag gcagacctct cagcagggtc ctttgaacga tttgtgaatc cattaaagac
     3121 gatgaatacc gagacacagg aaattttctt tgaattagaa gatcacgtaa agaaagatcc
     3181 acaaatcttt gacaatgaac agtatttaaa tgatctcaat gaacgtttaa aagcgaagga
     3241 ttcctatctg atcgtccgta aaaatggtca gatccaatat atagggaaga aaaaagtatc
     3301 tgataaatta attcataaac ttccatccta tgggaataaa gacagtgatg cagaccgtgg
     3361 tttttttgtc agcagaccag gaaactatct tgtaaaacag caggacttta aatacaatga
     3421 tggcggacaa ggatctgttt ttatcatgac agatcttgga acagtacttc cacattaccg
     3481 caatattatt attcaggtat cctgcgctgt gataggggta ctgatcttaa caagtacctt
     3541 tttatcatgg tttatgtatc gtgaattcgt cggaccgatc cgtgaattaa aagcaggtgc
     3601 agaacgtatc aaggaaggaa atcttgacaa cgatgtagtg attaacagtg gtggagaaga
     3661 gatcagggaa ttgtgcaatg catttaatga gatgagggca gaattgaaag actcgatcga
     3721 tgcaagaatt gtttatgaga agcaaaacag agatttgatc agtaacattt cccatgacct
     3781 gaagacgccg atcacagcga tcaaaggata tgtagaaggg atcatggatg gtgtagccga
     3841 tacacctgag aagatggatc gttacattaa gacggtctat aacaaggcaa atgatatgaa
     3901 tgtattgatc aatgaattat cattatactc taagatcgat tcgaatatca ttccgtataa
     3961 ttttgagaag atcaacatca attcgtattt taaagactgt gtcgatgaga tcggagcaga
     4021 ccttgagcaa agaggcatga tgttctctta tcgcaattac tgtgcagcca atgtcatggt
     4081 aacagcagat ccggaacagc ttaagagagt gataaacaat attatcaata atgcatgcaa
     4141 atacaataac aaagcaaaag gaaaagtagg aattacgtta acagaatatg accgaaaggt
     4201 acaggtagcg atcaaggaca atggaatcgg aatttccaaa gaagatcttc cacatatttt
     4261 tagaagaacc tatcgtgcag atatgtcgag aaattcagca ggtggaagtg gtcttggatt
     4321 atctatttgt aagaagatca tcgaagaaca tggcggtgag atctgggcag agagtaagct
     4381 gcgtgctgga acgacaatat tctttacact aaataaagta atcgatcagt caaaggagaa
     4441 ataacatgag caaagtatta atcgtagaag acgaactttc aattgcagaa ttagagaaag
     4501 attatctgga attaagttct tttgaagtag aaatcgaaac agatggacaa cagggacttg
     4561 agaaggcttt aaaaaatgat tttgatatga tcattttaga tatcatgtta cctggagttg
     4621 atggatttga gatctgtaag cagattcgag aagcaaaaga aattccgatc cttatggtat
     4681 ctgcaagaaa agaagatatc gataagatcc gagggttagg acttggtgca gatgattata
     4741 tcacaaagcc atttagtcca agtgaacttg tagcaagagt gaaggcacat ctgaatcgtt
     4801 atcagcgcct tgtaaataat cagatggaag tcaatgatat cattgagatc cgtggattga
     4861 agattgataa gactgcaaga cgtgtatact taaatggaga ggaaaaagtc tttacaacga
     4921 aagaatttga tcttttaaca ttccttgcag gacatccaaa cagagtcttt accaaggaag
     4981 aattatttaa taagatctgg gatatggaat ctttaggaga tatcgcaacc gttacggttc
     5041 atatcaagaa gatcagggag aagatcgaag ttaacacagc aaaacctcaa tatatcgaga
     5101 caatatgggg tgttggatat cgtttcaaga tgtaggagat ttttatgaga aagaataaaa
     5161 agataaatat catatataaa gatgaccaga tcgttgtctg tgagaagcca aaaggaatcc
     5221 ccgtttccag tgacaagacg ggcgacatgg atcttttgca tcagcttaaa aatcaactgt
     5281 tttttgagga aaatacagat ggggagccac agctttattt agttcaccgc ttagaccgtc
     5341 cagttggcgg ggtaatggta tttgcaagaa ccaagaaggc agctgcatct ctgacagaac
     5401 agatcaaaga tcatactttt gagaaagact atcaggcagt attgaccgga tggctgcctg
     5461 acgacgaagg gacattcagg gattatttat taaaagatcc gaagaataat gtatccaaaa
     5521 ttgtcaaaga aggaacgcca ggagccaaag aagcgatcct tgactatgaa gttattgata
     5581 tgatggaaga ttccaagatg aaatacacgt attgtctgat ccatttagag acaggccgtc
     5641 accatcagat ccgtgttcag acatcaagca gaggatttgg aatctgggga gatacaaagt
     5701 ataatccaat ctatcaaaag acaaagaaga aatacaaaga gatcggtcta tttgcagcac
     5761 ggatcgcatt tgaacatcca acaacaggtg agagaatggt atttaaagca gatccaagtg
     5821 gagaagcatt tgacttatta gagatgacag aataaacagg agatgataag aaaagatgag
     5881 atttagacca tgcattgaca ttcacaatgg caagatcaaa cagattgtag gaggaagtct
     5941 tcttgatcag ggagattttg caacagagaa ttttgtatcc gaaaaagatg gtacatttta
     6001 tggaaaatta tataaagaga atcatttgaa tggtggacat atcatcatgc tgaatgccag
     6061 agattcgaag tactatgaag aagataaggt acaggcatta aaggcgttga aagcatttga
     6121 agggggtctt cagatcggtg gagggatcac agatgagaat gcgaaagaat tcattgaatc
     6181 tggcgcatca catgtgatcg taacatcttt tgtatttgca ggtggaaagg ttcattatga
     6241 cagactggat gctctaaaag aacaggtagg aagagaacat ctggtacttg accttagttg
     6301 tcgaaagaga gatgacaaat actatatcac aacagaccgc tggcagaagt ttacggaaga
     6361 agaattaacc atagaacttc tgcacaaact ggaatcatgg tgtgatgaat atctgattca
     6421 tggagttgat gtagaaggaa aagcacaggg gatcgatcag gaactcgtgc aattattagc
     6481 tcggtataat ggcaataaag tcacatatgc aggtggagtt aagaattttg atgatctgga
     6541 attgctgaaa aactatggaa atgataagat tgatgtgacg atcggaagtg ccttagactt
     6601 atttggtgga gatatggaat tttcgaaagt cttagaatat tgcgatgcaa cgataacaaa
     6661 tctgaattaa gtaatacttt cacatggagt ggaaagagta tggaagggag gcttgaatat
     6721 atgaaactat tatatgaagc cagcaatttt acgatacaag caagatgggt cattccattt
     6781 gctattttag gaattatagg agtattactg ctcttatgtt cacggaaacc attttttaag
     6841 ttgttagctg cagtgtgtct tgtaatctgt gcgatcgcag cacttagatt ctgttttgga
     6901 tatgtgcagg tgatccaagc atataagcaa ggaaattaca agagtgttga aggatatgta
     6961 gagaattttg taccagcatc cagcggtgga gaagatcagg aagatatgga agaagaaagc
     7021 tttgatatta agggtgttca tttttcctat ggtgataaat ttattaagaa gtttggttac
     7081 cacagaccat cgacacaggg tggatatatc aagaaagacg gtcagtatct caagatcggt
     7141 tatatttcaa cggaaaaagg atatgtcatc gttaagattc aggaaagaaa ataaaagaat
     7201 attttacaac aaaaaatacc ggaaactttc aaatgataag tttccggtat tttgcatttt
     7261 agattcttat acaataataa gaaattaatt cttacgcata aattcagcac tcttcttagt
     7321 aagttttgca cgcagaccac gtttttcagg ttcaggtggt gcaacattac gaagattctt
     7381 gatggatgtg atataaatac cactgacaac agctttgatc tcagccttta caggaacgtc
     7441 atcgaagatt gcctcatcac acaataatgg agtgatcttt ggtccaatct ttgctttaac
     7501 aactggtacc ttagacatct tagcacgttt cggaatgtta tcagttacca tcttaggaaa
     7561 tccagcttcc atgatcttca ttcgcttctt atctatgaca agcatattca ctgtctgtgc
     7621 catctgatct aactgcgcac gctgatcttc ctgttttgcc tgcatctttc taccaacaaa
     7681 atacagaaca ccaagaatga tcgcagcaat taccgcaat
//
LOCUS       DAOMUE010000067         7612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2526_length_7612_cov_1.957920, whole genome shotgun sequence.
ACCESSION   DAOMUE010000067 DAOMUE010000000
VERSION     DAOMUE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2526_length_7612_cov_1.957920"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1202
                     /locus_tag="PLC71_04460"
     CDS             <1..>1202
                     /locus_tag="PLC71_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HRM01929.1"
                     /translation="RMIRQQEETEKKKENERQRELKLLEFLRQADEIETQYDHLQKEE
                     LKLHVQTEKNHELCKEKEAYFEKLRIYEEAGEVYDKIRMQRKEYRDRLSDWQDRYDCN
                     QAGLLARNLIDGHPCPVCGSLHHPKTADFKESDITQEMLRALREETELIDRQYSTAFA
                     KVKQSKGIVEICKEQLCKKSGKRSEEFEKLDEIYEISLRQYKKVKKEFERIKKQKEKI
                     AEQRVKVEKSKEKQLNYVEKLQEIAGKSHEKDSLYKQTEARIEEICKNLEYKTYKDAS
                     EEQQRLSDEIDQMKFNKEEIEKKKESVEIQGVHIKAAIEEKENTLSSVTKQLEEHKEM
                     NEEISDFRKFETKLHQMKKEIQKQNQNLEQLNQHISVNEQSIRFMDKKMEEYKETEMN
                     YTLLKDLS"
     assembly_gap    1203..1217
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1218..1721
                     /locus_tag="PLC71_04465"
     CDS             <1218..1721
                     /locus_tag="PLC71_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HRM01930.1"
                     /translation="GEQKGKTKISFERFVQSVYFDLILAAANERLSVMSEQRYYLLRK
                     EENDINKGSSGLELEILDEWTGKRRNVRSLSGGESFKAALSLALGLSDVIQNKKGGIQ
                     VDTIFIDEGFGTLDADSLNKAMQIINSLSLEGNKLVGIISHVDELKDQIDQKIEVYKD
                     HAGSKVK"
     gene            1741..1926
                     /locus_tag="PLC71_04470"
     CDS             1741..1926
                     /locus_tag="PLC71_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01931.1"
                     /translation="MTGSRRHDILGKKENNLSNIRTLYRFENREELMQAEADQIVVTT
                     TELLDVIWNHRSEKLYI"
     gene            2099..2905
                     /gene="spo0A"
                     /locus_tag="PLC71_04475"
     CDS             2099..2905
                     /gene="spo0A"
                     /locus_tag="PLC71_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HRM01932.1"
                     /translation="MNKINVAIADDNQRTVEMMTELLEQESDIEVIASADDGEEALRI
                     IKEKQPDVVLLDIIMPKLDGIGVLERLQTEDLSKRPIIIMVSAMGQENVCEEAMELGA
                     SYFILKPFDLRTIIKQIKQAKIKQQIPERPFVSVPEKIHGLTENQLEIIITNMIHEIG
                     VPAHIKGYQYLRDSIMMAVCDMDILNSITKQLYPSIAQKFDTTPSRVERAIRHAIEVA
                     WGRGNMDTIDALFGYTIHAGKGKPTNSEFIALIADKIRLEYGDEVLIEEK"
     gene            2947..3765
                     /gene="spo0A"
                     /locus_tag="PLC71_04480"
     CDS             2947..3765
                     /gene="spo0A"
                     /locus_tag="PLC71_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HRM01933.1"
                     /translation="MEKKLNILIVDGDVSEAEELENELNREFDIVGTVFDGMKAVDEI
                     IRKKPEVVLIDIMLPYIDGVGVIEKCRECMEYEQLPVFIVLTSIGTEKLIECVNHMDI
                     DYCMMRPFKNEVLCRRIKQVAKIKGLDQKIIQKETENNFKKNDQRTVIDHEFHMKQDV
                     TKIIRDLGIPAHIKGYQYIREGIIMAIEDINMMNYITKLLYPTIAKKYKTTSSSVERA
                     IRHAIEVAWSRGKVELLEEMFGYTISSGKGKPTNSEFIALIADKLRPDYHMNAS"
     gene            3834..4637
                     /gene="spo0A"
                     /locus_tag="PLC71_04485"
     CDS             3834..4637
                     /gene="spo0A"
                     /locus_tag="PLC71_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HRM01934.1"
                     /translation="MDERHRVLIADSYPDEAEQLKKELSGKYNVISVRDNGVDTLDDI
                     IKLKPDLVVIDLMLSEIDGIGVIEKCRELIEPDKIPAFIALTMLENRDLMECIRRLKV
                     DYCMMKPFQAGILAERISQMIRIRSVNNDIQKNEKALHGRSKRMCSMCGTNEISFLVR
                     NLGVPAHIKGCRYMKYAILMAIEDCNRINYITKLLYPEIAKKCKTTTSSVERAIRHAI
                     DIVWIKGNQELLKEIFGTFVMEGQERPTNSQFIAAVADWMRLEHQIKVS"
     gene            4663..5670
                     /gene="fliB"
                     /locus_tag="PLC71_04490"
     CDS             4663..5670
                     /gene="fliB"
                     /locus_tag="PLC71_04490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
                     /protein_id="HRM01935.1"
                     /translation="MKEYIPDYYKDFQCIADKCKDSCCIGWEIMIDSKSYKKYQNVKG
                     EFRDRLMKGIDHEGTPAFHLDDRDRCVFLNQKNLCDIYIELGEDALCEICTQHPRFHN
                     EYGNIRQTGLGMACEEATRLMFETKEFGLCQIQGTNTESTDDFDESVLEIQLWILDLL
                     KKKENPVEQRIEQIFDVVQGIQDHLNQTGEILNTWKNDPIKKNHILSQMREETYIKNW
                     ISIYQELDFMEPKIQKMFCKMEPMPCMIKEQGIDESYMEHLMSYFIYRYFMKSCEDNN
                     LIDKVKFAILSCLIIEQMNEYCKRNHILKTPQEIARIYSKEIEYSQENMEIIFEELLF
                     G"
     gene            5758..6303
                     /locus_tag="PLC71_04495"
     CDS             5758..6303
                     /locus_tag="PLC71_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM01936.1"
                     /translation="MRIRKRKPIGKKTIKYIEKLYEAYSRKLFCIAKNHVKDDDLAQD
                     VVQSVFEKALVYPESILKVPEEEVFYFLTAITKNESFTLLKNEKNNEHESLTYEDGSE
                     GDFVEDPRDNYLQMIDLESLKQNLAALPRRHRDTILFRYVYGLKCKEIADLFRITERS
                     VKNRCREARELLRQMMEADEL"
     gene            complement(6358..7023)
                     /locus_tag="PLC71_04500"
     CDS             complement(6358..7023)
                     /locus_tag="PLC71_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01937.1"
                     /translation="MKRRIFKQFLSGILGILLLLSLAGCGQSTSDSKPDDTMEAFYDL
                     IIKQDTTSMTDLGIDDSEASDTLKTYQTSMISTLQKSFKNAGVTITKKQANEIYKAIS
                     SKLSSLDHKITVTGQDKKNATVKVSSQYINYLDIFKQAKQTTLDELKPLHIENLSDAK
                     KQLVSNVIEAFNSTDVSSDMHTQTFKLKLQKIKSGKNTLHIFFPDNYEEVGSKLMKNA
                     TNQ"
     gene            7187..>7612
                     /locus_tag="PLC71_04505"
     CDS             7187..>7612
                     /locus_tag="PLC71_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM01938.1"
                     /translation="MANKTYDANSISVLEGLEAVRKRPGMYIGSVSTKGLNHLIYEIV
                     DNSVDEFLAGYCDEITVSLNKDGTATITDNGRGIPVGINERTGKTAVEMVFTMLHAGG
                     KFGDGGYKISGGLHGVGASVVNALSTWLTVTVKQGGKVYQ"
BASE COUNT         2857 a          966 c         1695 g         2079 t
ORIGIN      
        1 aacgtatgat cagacagcag gaagaaacgg agaaaaaaaa agagaacgaa agacaaagag
       61 aattaaaact actagaattt cttagacagg cagatgagat tgaaacacaa tacgatcatt
      121 tgcaaaaaga agaattgaaa cttcatgttc agacagaaaa aaatcatgaa ttgtgtaagg
      181 agaaagaagc ttattttgag aaacttcgga tttatgaaga ggctggagaa gtctatgata
      241 agatcagaat gcaaagaaaa gaatacagag atcgattgtc agactggcag gatcgatatg
      301 actgtaatca agcaggactt ctggcaagaa atctgataga cggtcatcca tgtccggttt
      361 gtggatcatt acatcatcca aaaacagcag attttaaaga aagtgatatt acgcaggaga
      421 tgcttcgtgc acttcgtgaa gaaacggaat tgatcgatcg gcaatatagt actgcatttg
      481 cgaaagtaaa acagagtaaa ggtattgttg agatatgtaa agaacagctt tgcaaaaaaa
      541 gtgggaaaag atcagaagag tttgaaaagc ttgacgagat ctatgaaatc tctttacgac
      601 aatataaaaa agtaaaaaaa gaatttgaaa gaatcaaaaa acagaaagaa aagatagcag
      661 aacaaagagt aaaagttgaa aaatccaaag aaaaacaatt gaactatgtg gaaaaactgc
      721 aggaaattgc cggaaaatca catgaaaaag atagtttata taagcagaca gaggccagaa
      781 tagaagaaat ctgtaagaat cttgaatata aaacatataa agatgccagt gaagaacagc
      841 agcgattatc tgatgagatc gatcagatga aatttaataa agaagaaata gaaaagaaaa
      901 aagaaagtgt tgaaatacag ggagttcata tcaaagcggc cattgaagaa aaagaaaata
      961 cactttcgag tgttacaaaa cagttggaag aacacaaaga gatgaatgaa gaaatttcag
     1021 attttagaaa atttgaaaca aaacttcatc agatgaaaaa agaaatacaa aaacaaaatc
     1081 agaatctgga acaattaaat caacatatct ctgtaaacga acaatcgata agatttatgg
     1141 acaagaagat ggaagagtat aaagaaacag aaatgaacta tacactgtta aaagatctgt
     1201 cannnnnnnn nnnnnnngga gaacaaaaag gaaagacaaa gatttcattt gaacgttttg
     1261 tgcagtcagt atattttgat ctgatcttgg cagcagccaa tgaacggttg tccgttatga
     1321 gtgaacaaag atattatctg cttcgaaaag aagaaaatga tataaacaaa ggatcttctg
     1381 gtctggaatt ggagattctc gatgaatgga caggaaaaag aagaaatgta agatcattat
     1441 ctggaggaga atcatttaaa gcagcattat ctcttgcgtt aggattaagt gatgtgatcc
     1501 agaacaaaaa aggcgggata caggttgata cgatctttat tgatgaggga tttggtactt
     1561 tagatgcgga ttctttaaat aaggcgatgc agatcataaa ttcactgtct ttggaaggaa
     1621 ataagctggt tggcataatt tcacatgtcg atgaattgaa agatcagatc gatcaaaaga
     1681 ttgaagttta taaagatcat gcaggaagta aagtgaaata aaaaggaatt gcaaattctt
     1741 atgacaggaa gtcgaagaca tgatatacta ggaaagaaag aaaataatct gtcaaacatt
     1801 agaacattgt acagatttga gaacagagag gagctgatgc aggccgaagc agatcagatt
     1861 gttgtgacaa caacagagtt actggatgtc atatggaatc atcgaagtga gaaattatat
     1921 atttaacaat ttacgcagcg aaatatgaca tagattttaa aaaatagaat taattttgtc
     1981 gaattatgtc gaatcctttg gaaatcaacc aaaacttgcg aagaaatatg ggttgtttgt
     2041 aaacttaaat caaatatata attaatttgt agagaaaaac agaatgggag gactgaagat
     2101 gaacaagatt aatgtggcta ttgcagatga caaccagaga actgttgaga tgatgacaga
     2161 actgttagag caggaaagtg atattgaagt aatcgcaagt gcggatgatg gagaagaagc
     2221 attgcgtatc atcaaggaaa aacaaccgga tgttgtatta ctggatatca ttatgccgaa
     2281 actcgatgga attggtgtat tagaacgttt gcaaacagaa gatctaagta aaagaccgat
     2341 cattattatg gtctccgcaa tggggcagga aaatgtctgc gaagaggcta tggaattagg
     2401 agcatcttat tttattttaa agccgtttga tctaagaacg atcattaagc agatcaagca
     2461 ggcaaagata aaacagcaga ttcctgaaag accattcgtt tctgtaccag agaagattca
     2521 tggattgaca gaaaaccagt tggagatcat cataacgaat atgattcatg agatcggggt
     2581 tccggctcat atcaaaggtt atcagtactt aagagattcg atcatgatgg cagtctgtga
     2641 tatggatatt ttaaattcca taacaaaaca gctgtatccg tcaatcgcac agaagtttga
     2701 tacgacacca agcagggtag aacgagcgat cagacatgcg atcgaggttg cctggggaag
     2761 aggaaatatg gacacgatcg atgcattgtt tggctatacg attcatgcag ggaaaggaaa
     2821 acctacgaat tcagagttta ttgcattgat cgcggataaa atacgcctgg aatatggaga
     2881 tgaagtactg atcgaggaaa aataacagca taagacatca taatcatgat caggaggttg
     2941 aaaaagttgg aaaagaagtt aaatatttta attgtggatg gggacgtatc agaagcagag
     3001 gagttagaaa atgaactgaa tagagagttt gatattgttg gaacagtatt cgatggaatg
     3061 aaagcagttg atgagatcat tagaaagaaa ccagaagttg tcttaattga tatcatgctg
     3121 ccatatatcg atggagtcgg ggtgatcgaa aaatgcagag aatgtatgga atatgaacaa
     3181 ttaccagtat ttattgtttt aacatcgatc ggtaccgaga aattgatcga atgcgtcaat
     3241 catatggaca ttgattattg tatgatgaga ccatttaaga atgaggtact gtgccgcagg
     3301 atcaaacaag ttgcaaaaat caaaggattg gatcagaaaa tcattcagaa agaaacagaa
     3361 aataatttta agaaaaatga tcaaagaaca gtgatcgatc atgaatttca tatgaaacag
     3421 gatgtgacaa agatcataag agatcttgga attccggctc atataaaggg gtatcagtat
     3481 attagagagg gaattatcat ggcaattgag gatattaata tgatgaatta tattacaaag
     3541 ttattatatc caacaattgc gaaaaaatac aaaacgacat caagcagcgt agagagggcg
     3601 attcggcatg cgattgaggt ggcatggagt cgtggaaaag ttgaattgct ggaagaaatg
     3661 tttggttata cgataagttc tggaaaggga aagccgacaa attcagagtt tattgcattg
     3721 atcgcggata aactaagacc cgattatcat atgaatgcat cttaagatga tgatgatgag
     3781 ataatataag atgcatatgg acgaatacag ggagattgtc tgaaggagaa aatatggatg
     3841 aaagacatag agttttaatt gcagacagtt atccggacga ggcagaacaa ttaaagaaag
     3901 aattatcagg aaaatataat gtaatatcag taagagataa tggggtggat acattggatg
     3961 atattataaa attaaaacct gatcttgtag ttattgatct gatgctttca gagattgacg
     4021 gaattggtgt aatcgaaaaa tgcagggaat tgatagaacc agataaaatt ccagcattta
     4081 ttgcattaac aatgctcgaa aatagagatc tgatggagtg tatcagacgg ttaaaagttg
     4141 attattgtat gatgaaaccg tttcaggcag gaattcttgc agagagaatt tcacagatga
     4201 tacggatacg ttcagtaaat aatgatattc aaaaaaatga gaaagcattg catggacgat
     4261 caaaaagaat gtgtagcatg tgtggaacga atgagattag ctttttggtt aggaatttag
     4321 gggtgcctgc acatattaaa gggtgccgtt atatgaaata tgcgattcta atggcgatag
     4381 aagactgtaa tcgtataaat tatattacaa aattattata ccctgagatt gcaaagaaat
     4441 gtaaaacaac gaccagtagt gttgaacggg cgatccgcca tgcgatcgat attgtatgga
     4501 tcaaaggaaa tcaagaactt ttaaaagaga tttttggaac atttgtgatg gaaggacagg
     4561 aacgtccgac aaattcacaa tttattgcag cagttgcaga ttggatgcgg ctggaacatc
     4621 agataaaagt ttcataagaa tgaaaatatt ttggaggata agatgaaaga atatattcct
     4681 gattattata aggattttca gtgcattgca gataaatgta aagatagttg ctgcattgga
     4741 tgggagatca tgatcgattc taagtcctat aaaaaatatc agaatgttaa aggagaattt
     4801 cgtgacaggc tgatgaaagg cattgatcac gaaggcactc cagcatttca tcttgatgat
     4861 cgtgaccgct gtgtgttttt gaatcagaaa aatttatgtg atatttatat tgaattagga
     4921 gaagacgctt tgtgtgagat atgtacacag catccgagat ttcataatga atatggaaat
     4981 attcgccaga caggtcttgg aatggcatgc gaagaagcta cacggctgat gtttgagaca
     5041 aaggaatttg gattatgcca gattcaggga acgaatacag agtcaacgga tgactttgat
     5101 gaatcagttt tagaaattca attatggata ttggatttgc tgaaaaagaa agaaaaccca
     5161 gtggaacaaa gaatagaaca gatatttgat gtggtgcagg gaatccagga tcatttgaac
     5221 caaacgggtg agattctgaa tacatggaaa aatgatccta taaagaagaa tcatatatta
     5281 agtcaaatgc gagaagaaac atatatcaaa aactggatat ctatatatca ggaattagat
     5341 tttatggagc caaagattca gaagatgttt tgcaagatgg aaccaatgcc atgcatgatc
     5401 aaagaacaag gaatcgatga gtcatatatg gaacatttga tgagttattt tatttatcgt
     5461 tattttatga aatcctgtga agataataat ctgatcgata aagttaagtt cgcaatttta
     5521 agctgtttga taattgaaca gatgaatgaa tattgtaaga gaaatcatat tttgaagaca
     5581 ccgcaggaga ttgctcgaat ttactcaaaa gagattgaat attcgcaaga aaacatggaa
     5641 atcatttttg aagagctatt atttggatag atcattatac aaacatttgt aatggaacaa
     5701 tatatcttta taatagttca gaaaaaaaca atttaagaaa attgaaagtg aaatattatg
     5761 agaattcgaa agagaaaacc aatagggaaa aagacgatca aatacataga gaaattgtat
     5821 gaggcatatt caaggaagtt attttgtatt gcaaagaatc atgtgaaaga cgatgatcta
     5881 gcacaagacg tggtgcaaag tgtatttgaa aaagcattgg tatatccgga aagtattttg
     5941 aaggtgccag aggaagaagt attttatttt cttacggcaa taacaaagaa tgaatccttt
     6001 acattgttga aaaatgaaaa gaataatgaa cacgagagtc ttacatatga agatgggtca
     6061 gaaggggatt ttgttgaaga tcctagagat aattatctgc agatgatcga tctggaatct
     6121 ttaaaacaga atctggcggc attaccacga agacatcgag atacgatttt atttcgatat
     6181 gtttatggat tgaaatgtaa agaaattgca gatttattta gaattacaga gcgaagtgtt
     6241 aaaaatcgct gcagagaagc aagggaactg ctcaggcaga tgatggaagc agacgaattg
     6301 taattaggat caatataaaa aggttagcag tgtgatcata ctgctaacct tttatattta
     6361 ttgatttgtt gcatttttca tgagtttaga tccgacttct tcataattgt ccgggaagaa
     6421 gatatgaagg gtatttttac cgcttttgat cttctgtagt tttaatttaa atgtttgtgt
     6481 atgcatatca gaggatacat cagtagaatt gaaagcttcg atcacattgg atactaattg
     6541 tttctttgca tcggataagt tctcaatgtg aagaggtttt aattcatcca aagtagtctg
     6601 ttttgcttgt ttaaaaatgt ccagataatt gatgtattgg ctagacacct taactgttgc
     6661 atttttctta tcttgacctg ttaccgtgat cttgtggtct aaggaagaaa gtttgctgct
     6721 gatcgcttta tagatttcat ttgcttgttt cttagtgatc gtaacgcctg cattcttaaa
     6781 actcttctga agagttgata tcatggatgt ctgatatgtt tttaaagtat cagatgcttc
     6841 agaatcatca attccaagat ctgtcatcga tgttgtatct tgttttatga tcagatcata
     6901 gaatgcctcc atggtgtcat caggttttga gtcacttgtg ctctgtccac atccagcaag
     6961 ggataacagt aataagatcc ctaatatacc ggataggaac tgtttgaaaa tcctacgttt
     7021 catacttatt gttcctttct agtttattca tatctcatta tagagatttt ggcaggtaaa
     7081 gtcaattact gtcgaatgat ggctataaga aacagctcaa ttgacgaaga agatcattgt
     7141 gtgatacact agataaccga aagagaaaaa ggagagttgc gtatttatgg caaataagac
     7201 atatgatgct aatagtattt cagttttaga aggactagaa gctgttagaa aacgccctgg
     7261 aatgtacatt ggaagtgtat ctaccaaggg attgaatcat ttaatatatg agatagtaga
     7321 caattcggtc gatgaatttc tggcaggata ttgtgatgag atcacagtat ctttaaataa
     7381 agatggaaca gcaactatta cggacaatgg acgaggaatt ccggtcggaa ttaatgaaag
     7441 aacaggaaaa acagcggtag agatggtatt taccatgtta catgcaggag gtaagtttgg
     7501 agatggagga tataagattt ctggaggact gcatggagtt ggtgcatccg ttgttaatgc
     7561 cctttcaacc tggcttacgg taactgtaaa acagggagga aaggtttatc ag
//
LOCUS       DAOMUE010000068         7469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2594_length_7469_cov_2.300647, whole genome shotgun sequence.
ACCESSION   DAOMUE010000068 DAOMUE010000000
VERSION     DAOMUE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2594_length_7469_cov_2.300647"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="PLC71_04510"
     CDS             <1..1017
                     /locus_tag="PLC71_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM01939.1"
                     /translation="SIGDNVYTTLNTKVQKAAYYALQGQKGAVVAMDPETGKIYAMVS
                     KPDYRPAYVKENWTELNTSKNSLLLNRATQGLYPPGSTFKVVTALEYMREHKNTYKNF
                     KYHCTGYTTVGTLKIHCYGGEAHGTVNLEQAIEKSCNCAFVHMSEEINKGKLASLAGD
                     LMYNSKVPINLLANSSRFVLDKKSGTNEMAHTSIGQGQTLITPLENAMVMSAIANDGV
                     VMQPYLVENVKNIDGGVIKETKASKLSEPLEASECETLKKMLRKVVTKGTGTRAYSSH
                     YNVYGKTGSAEYNSAKDSHAWFIGCAERGGKKIVVSVLVEGGDSGGRVAAPIADKVFH
                     AFLD"
     gene            1143..1592
                     /locus_tag="PLC71_04515"
     CDS             1143..1592
                     /locus_tag="PLC71_04515"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRM01940.1"
                     /translation="MTKATSCGGVVIFRGKILLLYKSYKNRYDGWVLPKGTVEQGETH
                     EQTALREVKEETDADASIIKYVGKSEYSFYSFHEQIIKSVHWYLMESENYHSKPQKEE
                     FFVDSGYYKFHEAYHLLKFPNEKEILKQAYDEYKKLRSANRWGKKTR"
     gene            1727..2926
                     /locus_tag="PLC71_04520"
     CDS             1727..2926
                     /locus_tag="PLC71_04520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRM01941.1"
                     /translation="MQKWKVILLVAAILVQSFYCPVMAREARFLETAAPVLDLSAKSA
                     VVMEQESKEILFAKDKDKELAPASVTKIMSLLLIFEDLKQGKIKLTDTVTVSEHAASM
                     GGSQVFLETGEKQQVQTLIKCVVISSANDAVVALAEHVCGSEEAFVSRMNKKARELGM
                     KHTTFQNACGLDAKGHVTSAYDIALMTRELAQRYPEVFKYTKIWMDTIIHKTKKGEKE
                     FGLSNTNKLIRQYHGCTGMKTGSTGKAGFCLSATATRNKIHMIAVVMGCESSKARIKD
                     ARTLLDYGFSNCQRIHSETTRKEIGRIPVQKGQRSEVSCQEKVTADLIDIKTQKGKIV
                     KKIKIDSVKAPVKKGQKIGEIQYSKGNIMIHKEKILAQETINKADFLTQIKKISTQYF
                     LILSHFL"
     gene            3038..3853
                     /locus_tag="PLC71_04525"
     CDS             3038..3853
                     /locus_tag="PLC71_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003370065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM01942.1"
                     /translation="MSESWLNLEGKTVIVTGGASGIGKAVAQGFLNAGSNVVISDMAP
                     EAPEMDEAAGKLLYVKTDVTKVADVEAMVEKAKETFGTIDVLVNNAGINIPRLLVDPK
                     DPKGQYELDEAVWDKVCNVNLKGVFFCAQAVGRVLVEKGEGVIINMSSESGLEGSEGQ
                     SVYAATKNAVNSLTRSWAKELGKQGVRVVGVAPGIMEATGLRTIAYESALAYTRGITV
                     DDLRAGYSKTSTIPLGRSGKLSEVADMCVYLASQRASYVHGVTVNVAGGKTRG"
     gene            3891..>4241
                     /locus_tag="PLC71_04530"
     CDS             3891..>4241
                     /locus_tag="PLC71_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HRM01943.1"
                     /translation="MKQLFNNENRTAVILRNIEMQSVCSVESLAAKLDVSQKTIRNDI
                     KELNCLLGGYACISNNKGTCKLIVFAPKGFTEIRNKIYKQEDLFNSSHTRMAYMFWQL
                     MHAEEPYLTDDLSEE"
     assembly_gap    4242..4251
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4252..4871
                     /locus_tag="PLC71_04535"
     CDS             <4252..4871
                     /locus_tag="PLC71_04535"
                     /inference="COORDINATES: protein motif:HMM:NF016901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM01944.1"
                     /translation="TIGDLNRLRKAIEKYDLKIKGKANTGLALCGDEFNIRLFILENI
                     YEQLYLNFPLGQTIREKLYDFQERLSMDALGFGFFYRFFVVMIQRMESGHTIKKLKPK
                     YEELYESSAYMIVDEFLNEIEQVKGYKISKEERLFLSISVAGMRTPANTAEIEQKISI
                     SEGVADLIIEILDRIKAELNVTVVANELFDDFVYHVFFMINRLNL"
     gene            4918..5790
                     /locus_tag="PLC71_04540"
     CDS             4918..5790
                     /locus_tag="PLC71_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM01945.1"
                     /translation="MAEIAKGVLEERIGIEMTEDEMGFLAAYFGVFLLEQEPEKKRCK
                     IAIVCGSGKIIGRLIENQLKKIFDVEPEFEFFYGIFDENRKDDFDYIVTTTELHMDTK
                     TPVIFMDEVFDREYIQRKFENMRYLSEAGRTIRKGIDSLFMNLLDETRFFVLDQESSY
                     DENVDRMARALTNQGELDAGFAQRVREREKYSTMVLDQNIAFPHTKNMLSKLTLAMGV
                     FPDMLKDDKYGNIRIIILLGIPESMEDDTVLVRLYDDILSIGKKPDVIPKIQKMESYR
                     EFLLYIAEENNIFE"
     gene            5809..6258
                     /locus_tag="PLC71_04545"
     CDS             5809..6258
                     /locus_tag="PLC71_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HRM01946.1"
                     /translation="MSFMVIAVAMLAAFCLQFLFQMVQMKSFNKFYTKLRKKGRVAIG
                     KAKGGFHAGAIVMFAIDQDGVIIDGCFMRGFTMLARFKNWDFFNGIDVGTIKKDDCAT
                     IGVSLRRAVLNASSNYNTIMSGGEVLEKPSLFTRIGDKIYGVNKKKA"
     gene            6276..6818
                     /gene="srlA"
                     /locus_tag="PLC71_04550"
     CDS             6276..6818
                     /gene="srlA"
                     /locus_tag="PLC71_04550"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol/sorbitol-specific PTS transporter
                     subunit IIC"
                     /protein_id="HRM01947.1"
                     /translation="MNIIIKGASGFMGLFDTGAETFISWVSGIVPKVLLLLILMNTII
                     ALIGQERVNKFAKLCSGNVILAYGVLPFISAFMLGNPMALSMGKFLPERMKPSYYASA
                     SYHCHTNSGIFSHINPGEIFIYLGIAQGVQKAGFDMTELGLRYMLVGLVMNFFSGWVT
                     DFTTKMVMKQQGIELSNELK"
     gene            6853..>7469
                     /locus_tag="PLC71_04555"
     CDS             6853..>7469
                     /locus_tag="PLC71_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HRM01948.1"
                     /translation="MSEYRAITITQGSGGFGGPLTVEPKEGKDVLLYITGGGAEPDIL
                     PKIVELTGCRPVNGFKTSVPEEEIFLVIIDCGGTLRCGIYPQKRIPTININPVGKSGP
                     MAKYITEDIYVSGVKNDNIALADGSAAPISEAAPAKEEKDFKYSADKKVSETMGSSSK
                     SSFIQKIGMGAGKVVATFNSAAKESTNTVLHTIIPFMGFVSLLIG"
BASE COUNT         2667 a         1124 c         1685 g         1983 t
ORIGIN      
        1 agtatagggg ataatgtata cacaacatta aataccaaag ttcagaaagc agcatattat
       61 gccttgcagg gacaaaaggg agcggttgtt gcgatggatc cagagacagg aaagatctac
      121 gcaatggtat ccaaaccgga ttacagacca gcttacgtca aagaaaactg gacagaatta
      181 aatacaagca agaattcttt gttgttaaac cgtgcaacgc aaggattata tccgccagga
      241 tcaacattta aagttgtgac ggcattagaa tatatgagag aacataaaaa cacatataag
      301 aatttcaaat accattgtac tggatataca acggttggaa ctttaaagat tcattgttat
      361 ggaggagaag cgcatggaac ggtgaatctt gaacaggcaa tcgagaaatc ctgtaactgt
      421 gcgtttgtgc atatgtcaga agagattaac aaaggaaaac tcgcaagtct tgcaggagat
      481 ctgatgtata attcgaaagt tccgatcaat cttcttgcaa acagcagtcg ttttgttctt
      541 gataagaaat caggaactaa tgagatggca cacacttcga tcggacaagg tcagactttg
      601 atcacaccac ttgaaaatgc gatggtcatg tctgcgatcg ccaatgatgg agttgtgatg
      661 cagccatatc tggtagagaa tgtcaaaaat atcgacggtg gagtgatcaa ggaaacaaaa
      721 gcatcaaaac tatcagaacc tttagaggca tcggaatgtg agacattaaa gaaaatgttg
      781 agaaaagttg tcacaaaggg aactggaaca agagcttatt cttcacacta taatgtatat
      841 ggaaagacag gatcagcaga atataacagc gcgaaagatt cccacgcatg gtttatcgga
      901 tgtgccgaaa gaggcgggaa gaagatcgta gtcagtgttc ttgtagaggg tggagactct
      961 ggaggacgag ttgcggcacc gatcgcagat aaagtatttc atgcattttt agattgatgt
     1021 tttcaaaaga gtaaaacaga tagcagttga aatcagttca taatccatgt ataatatttg
     1081 tcagagaaag acacacatac ttaggcgaat aagatcgttt aagagacagg aggttgtaac
     1141 tggtgacaaa agcaacgagc tgtggtggtg tcgtaatatt tagaggaaag attttgctgt
     1201 tatacaaaag ttataagaac cgatatgatg gctgggtact tccgaaaggt actgttgagc
     1261 aaggagagac tcacgagcag acagcacttc gtgaggtaaa ggaagagaca gacgcggacg
     1321 catcaattat caagtatgta gggaaaagtg aatattcttt ctattcattt catgaacaga
     1381 tcataaagag tgtacactgg tacttgatgg aatccgagaa ctatcactca aaacctcaga
     1441 aggaagagtt ttttgtagac tcagggtatt acaaatttca cgaggcttac cacttattga
     1501 aatttcctaa tgagaaagag atcttaaaac aagcatatga tgaatataaa aagttgagat
     1561 ctgcgaatcg ttggggtaag aaaacaagat aaatgaagga aaatcagaga tatgatcaga
     1621 agatatttct ggttttcttt tttaaaaaaa catatacagc aaagaaatta tgtataaatc
     1681 taagtatctg ccactaaact ataacaaaca ggtttgaggt ggatgcatgc agaaatggaa
     1741 agtgatactg ctcgttgcag caatactcgt tcaaagcttt tattgtccag tcatggcaag
     1801 agaggcaaga tttttagaaa cagcagcacc agtacttgat ctttcagcaa aatcagcagt
     1861 agttatggaa caggaaagca aggaaatctt atttgcgaaa gataaggata aagaacttgc
     1921 accagctagt gtaacgaaaa taatgtcctt gctgcttatt tttgaagact taaagcaagg
     1981 taagatcaaa cttacagata cggttacagt cagtgaacat gcagcatcta tgggcggctc
     2041 tcaggtgttt ttagaaacag gagagaagca gcaggtacaa acattgatca aatgtgttgt
     2101 gatctcaagt gcgaatgatg cagttgttgc attggcagag catgtctgcg gaagtgagga
     2161 agcgttcgta tcaaggatga acaaaaaagc cagagagctt ggaatgaaac atacgacatt
     2221 tcaaaatgcc tgcgggcttg atgcaaaagg acatgtgaca agtgcatatg atattgcgtt
     2281 gatgacgaga gaacttgcac aaagatatcc agaagttttt aaatatacaa agatctggat
     2341 ggatacgatc atacataaaa cgaaaaaagg tgagaaagaa tttggtttaa gtaacacaaa
     2401 caaactgatc cgccagtacc atggatgtac aggaatgaag actggaagta cagggaaagc
     2461 aggattttgt ctaagtgcaa ctgcaacaag aaataagatt catatgattg cggtagtgat
     2521 ggggtgtgaa agctctaaag caaggatcaa agatgcaaga actcttttgg actatggatt
     2581 ttcaaattgc cagcggatac acagcgaaac tacaagaaaa gaaatcggaa ggattccagt
     2641 acaaaagggt cagagatcag aagtatcttg ccaagaaaag gtgactgctg atctgatcga
     2701 tattaaaaca cagaagggaa agatcgtgaa aaagatcaag atagattctg tgaaagcacc
     2761 agtgaagaaa ggacagaaga ttggtgagat tcaatatagc aaaggtaata ttatgatcca
     2821 taaagaaaag atcctggcac aagaaacaat aaataaagca gattttttga cacagattaa
     2881 aaaaatttcc actcaatatt tcttgatttt aagccatttt ttgtaataag tccacaaaat
     2941 ttcttcacga tggaaaatga aagaattaaa tttttccttg acgctctata taatgaactc
     3001 atgaagcaaa gaaacacaga ttcaaaggag gattataatg agcgaatctt ggttaaatct
     3061 tgaaggaaaa acagtcatcg taactggtgg agcatctgga atcggaaaag cagttgcaca
     3121 gggattctta aatgcaggat ctaacgtagt catttctgat atggcacctg aagcaccaga
     3181 gatggacgaa gcagcaggaa aattactgta tgtgaaaaca gatgttacaa aagtagcaga
     3241 tgttgaagca atggtagaaa aagccaaaga aacattcgga acgatcgacg tattagtaaa
     3301 caatgccgga atcaacatcc ctcgtttgtt agtagatcca aaagatccaa aaggacagta
     3361 cgaattagat gaagcagtat gggataaagt ttgcaatgta aacttaaaag gtgtattctt
     3421 ctgtgcacag gctgttggtc gtgtgcttgt tgaaaaaggt gaaggagtta tcattaacat
     3481 gtcttctgaa agtggtctgg aaggatctga aggacagagc gtttacgcag caacaaagaa
     3541 cgctgtaaac tctttaacaa gatcttgggc aaaagaactt ggaaaacagg gagtcagagt
     3601 tgtcggagta gcacctggaa tcatggaagc tacaggactt cgtacaattg cttatgaatc
     3661 agcattagca tatacaagag gaatcacagt agatgactta agagctggat attctaaaac
     3721 atctacaatt ccactaggaa gatctggtaa attatcagaa gttgcagata tgtgtgtata
     3781 tcttgcaagt cagagagctt cttatgttca tggagttaca gtgaacgtag caggaggaaa
     3841 aacaagaggt taaaaacaat taacctgtat caatagaaaa gaggatcaat atgaaacagc
     3901 tttttaacaa tgaaaacaga accgcagtta ttttaaggaa tatcgaaatg caatctgttt
     3961 gcagtgtaga aagcctcgcg gcaaagcttg atgtgtcgca aaaaacgatc cgcaatgaca
     4021 ttaaggaatt gaattgcctg cttggaggat acgcatgtat cagcaataac aaaggaacat
     4081 gtaaactgat cgtgtttgca ccgaaaggtt ttacagaaat ccgcaataag atttacaaac
     4141 aggaagacct ttttaattcc agtcatacaa gaatggcata tatgttctgg cagctgatgc
     4201 atgccgagga accgtatcta acggatgatc tttctgagga gnnnnnnnnn ncaacgatcg
     4261 gtgatctgaa ccgattaaga aaggcgattg agaaatacga cctgaagatt aaaggaaaag
     4321 caaatacagg actggctctt tgtggtgatg agtttaacat tcgcctattt atcttagaaa
     4381 atatttatga acaactgtat ttaaatttcc cacttggaca gacaattcgt gaaaaactat
     4441 atgattttca ggaaagatta tcgatggatg ccctagggtt tggtttcttt tacagatttt
     4501 ttgttgtgat gatccagaga atggaaagcg gtcatacgat caaaaagctg aagccgaaat
     4561 atgaagaact atacgaaagt tcagcataca tgatcgtgga tgagttcctt aatgaaattg
     4621 aacaggtcaa aggatataag atatccaaag aagagcgatt gtttttaagt atatctgtag
     4681 caggaatgag aacaccggca aatacagcag agatcgagca aaagatcagt atttcggaag
     4741 gtgttgcaga tcttatcatt gaaattttag atcggatcaa agcagaactt aatgtaacag
     4801 ttgtagcaaa tgaattattt gatgattttg tctatcacgt attttttatg atcaaccggc
     4861 taaatttata atccgatggt tgatgatttc aagaacaaat acagtgtagc ctataaaatg
     4921 gcggagattg caaaaggtgt tttggaagaa cgcataggaa tcgaaatgac agaagatgag
     4981 atgggatttc tggctgctta ttttggagtg tttttattag agcaggaacc agaaaagaaa
     5041 cgatgcaaga tcgcgatcgt ttgtggatct ggaaagatca tcggacgtct gatcgagaat
     5101 cagttaaaga aaatctttga tgtagaaccg gaatttgaat ttttctatgg tatcttcgat
     5161 gaaaatcgca aagatgattt tgattatatt gttacgacga cagaattaca tatggatacg
     5221 aaaaccccgg tcatttttat ggatgaagta ttcgatagag aatatattca acgtaaattt
     5281 gaaaatatga gatacctttc tgaagcagga cgaacaatcc gaaagggaat agattctttg
     5341 tttatgaatt tattagatga aacgagattt tttgttctgg atcaagaaag ttcatatgat
     5401 gagaacgttg atcggatggc aagggcactt accaatcagg gagaacttga tgcaggattt
     5461 gcccagagag tgagagaacg tgaaaaatat tcaacaatgg ttcttgacca gaatattgcg
     5521 tttccacata caaagaatat gctgtcaaaa ttaacattgg caatgggtgt gtttccagat
     5581 atgttaaaag atgataaata cggaaatatc cgaatcatta ttttactggg aattcctgaa
     5641 tcaatggaag atgacacagt acttgtccga ttatatgatg atattttgtc gattggcaag
     5701 aaaccggatg tcattccaaa gatccagaaa atggaaagct acagagaatt tcttctctat
     5761 attgcagaag aaaataatat atttgagtaa acgaaaagga ggaagacgat gtcatttatg
     5821 gtcatagctg tggcgatgct tgcagcattt tgtctgcaat ttttatttca gatggtgcag
     5881 atgaaaagtt ttaataaatt ttatacaaaa cttcgaaaaa aaggccgggt tgccatagga
     5941 aaggcaaaag gcggttttca tgcaggtgcg atcgttatgt ttgcgatcga tcaggatgga
     6001 gtgatcatag atggttgctt tatgagagga tttacaatgc ttgcacgatt taaaaactgg
     6061 gatttcttta atggaattga tgttggaacg attaaaaaag atgattgtgc aacgatcggt
     6121 gtatccttaa gaagagcagt gcttaatgct tcctcaaatt acaacacgat tatgagtgga
     6181 ggcgaagtct tagaaaagcc gagtctgttt acaagaattg gagacaagat ttacggtgtc
     6241 aataagaaaa aagcctgaaa tgaaaggaga attatatgaa tatcattatt aaaggtgcat
     6301 caggattcat gggattattt gatacaggtg cagaaacatt tataagctgg gtgtcaggaa
     6361 tcgtaccaaa ggtattatta ctgttaatcc taatgaatac gatcatcgcc ctgattggac
     6421 aggaaagagt taataaattt gctaaattat gttcaggaaa cgtaatcctt gcatatggag
     6481 tattaccatt tatttcagca tttatgttag gaaacccaat ggcattatca atgggtaaat
     6541 tccttccaga aagaatgaaa ccaagctatt atgcatcagc ttcttatcat tgccatacaa
     6601 acagtggaat tttctctcat attaacccag gtgaaatctt tatctacctt ggaattgcac
     6661 agggtgttca gaaagctgga tttgatatga cagaattagg tcttcgttat atgttagttg
     6721 gtcttgttat gaacttcttc tcaggatggg taacagactt tacaacaaag atggttatga
     6781 aacagcaggg aatcgaatta agtaacgaat tgaaataaga tggatttacg agtgtaatta
     6841 ggaggaaaaa ctatgtcaga atatagagcg attacgatca cacagggaag cggtggattt
     6901 ggtggtccat taacagtaga acctaaagaa ggaaaagatg tattattata tatcacaggt
     6961 ggtggagcag aacctgatat cttacctaaa atcgtagaat taacaggatg ccgtccagtt
     7021 aacggattta agacatctgt tccagaagaa gagatcttcc ttgtgatcat tgactgtggt
     7081 ggaacacttc gttgtggaat ttatccacag aagagaattc caacgatcaa tatcaaccca
     7141 gttggaaaat ctggaccaat ggcaaaatat attacagaag atatttatgt atctggtgtt
     7201 aaaaatgata acattgcatt ggcagatgga tctgcagcac ctatttcaga agcagcacct
     7261 gcaaaagaag aaaaagactt caaatacagc gcagataaga aagtatctga aacaatggga
     7321 agctcatcaa aatcaagctt tatccagaaa attggtatgg gagctggtaa agtcgttgct
     7381 actttcaata gtgcagcgaa agaatctaca aacacagtac ttcatacgat cattccattc
     7441 atgggattcg tatcattgct gatcggtat
//
LOCUS       DAOMUE010000069         7428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2613_length_7428_cov_2.359555, whole genome shotgun sequence.
ACCESSION   DAOMUE010000069 DAOMUE010000000
VERSION     DAOMUE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2613_length_7428_cov_2.359555"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLC71_04560"
     CDS             <1..586
                     /locus_tag="PLC71_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HRM01949.1"
                     /translation="NGDGFKVALLDLGTKNNIIRSLEKRGCDVTVYPADTTAEEILGD
                     QPDGIMLSNGPGDPKECVQIIKEIKKLYDSEVPIFAICLGHQLMALATGADTEKMRWG
                     HRGGNHPVKDLKSGRVYISSQNHGYVIKEETIDPDVAEVSFINVNDKTIEGLDYKNKN
                     IRTVQFHPEACPGPQDTAYLFDEFMDRMKGAKNA"
     gene            579..2985
                     /gene="carB"
                     /locus_tag="PLC71_04565"
                     /pseudo
     CDS             579..2985
                     /gene="carB"
                     /locus_tag="PLC71_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391991.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     assembly_gap    2986..2995
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2996..3766
                     /locus_tag="PLC71_04570"
     CDS             <2996..3766
                     /locus_tag="PLC71_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRM01950.1"
                     /translation="KSLHVIGLINIQFIVADDEVYVIEVNPRSSRTVPYISKVTGIPI
                     VALAAKVITGAKIRDLGYEPGLQKESEYYAVKKPVFSFEKLRGAEISLGPEMKSTGDC
                     LGISKNFHEALYKAFLGAGVNLPKYKKMILTVKDSDKIDAIDIGRRFEALGYEIFSTK
                     STCRVLNEAGVHAKQINKLEEESPNLLDLILDDKIDLVIDTPSQGVGRSRDGFLIRRY
                     AIETGVTCLTSLDTADALLTSLETGNRDQLSVVDIAQI"
     gene            3792..5123
                     /gene="glnA"
                     /locus_tag="PLC71_04575"
     CDS             3792..5123
                     /gene="glnA"
                     /locus_tag="PLC71_04575"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRM01951.1"
                     /translation="MGRYTKQDVIDLVRENDVEFIRLQFSDLFGTMKNVAITARQLEK
                     ALDNKCTFDGSSVDGFVRIEESDMYLYPDLDSFAIFPWNAGGNRVARLICDVYGQDGL
                     PFAGDPRNVLKRVMVECQKMGYKFNVGPECEFFLFHTDEEGRPTTVSHEKAGYFDVAP
                     LDLGESARRDMILNLEDMGFEIEASHHEVAPAQHEIDFKYDNALRTADNILTFKFIVK
                     YIAKRHGLYASFMPKPITGVDGSGMHLNMSLWKNGKNIFADSKNNLGISEEAFHFMAG
                     VMEHANEMCLITNPLVNSYKRIVPGYEAPVDVSWSPSNRSPLIRVPAARGSGTRLEFR
                     SPDSAANPYLALAVSLAAGLDGIKRKLTPGKGSMKNQYEMAEGEKQEAGINTLPRDMK
                     EALEIFKQSTYMKELLGDHIFNKYVEAKSAEWESYQKFVSQWEVDRYLYKI"
     gene            5150..5713
                     /locus_tag="PLC71_04580"
     CDS             5150..5713
                     /locus_tag="PLC71_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HRM01952.1"
                     /translation="MRSLVNVIVLFPKQEVARSIRNLLVRSGFEVTAVCATGAQVVQR
                     MEGVEEGLVVCGYKCSDMIYSELREYLSEEVKMLLIASRQYLDDCVYPNVIGLSMPLK
                     GYELVEKTREIVTEISEKQRRRKQRPKQRTREEVRLLEEVKELLMQNNQITESEAHAY
                     IQQRSMNSGVGLIEMAKMIKESLTYQN"
     gene            5723..6937
                     /locus_tag="PLC71_04585"
     CDS             5723..6937
                     /locus_tag="PLC71_04585"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRM01953.1"
                     /translation="MFKINDNYLKLPGSYLFSTIAKKVNAYSEANPDKNIIRLGIGDV
                     TQPLAPAIIDSLHKAVDEMGHAETFHGYAPDLGYEFLRNAIAKNDYQDRGADIKADEI
                     FVSDGAKCDSGNIQEIFAQDNKIAVCDPVYPVYVDTNVMAGRAGTYDKATETWSDVIY
                     MPCTAENNFAPDFPSEVPDIIYLCFPNNPTGSTITKDQLQGWVDYANKNGCVIIYDAA
                     YEAYISEDNVAHTIYECEGARTCAIELKSFSKNAGFTGVRLGYTVIPKDLKCGDTQLH
                     ALWARRHGTKYNGAPYIVQRAGEAVYSEAGKAQLKEQVGYYMNNAKVIFNGLQDAGYT
                     VSGGVNAPYIWLKTPDNMTSWEFFDHLLEDANVVGTPGSGFGPSGEGYYRLTAFGNYE
                     NTVEAIERIKRM"
     gene            7246..>7428
                     /locus_tag="PLC71_04590"
     CDS             7246..>7428
                     /locus_tag="PLC71_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HRM01954.1"
                     /translation="MKKLALAQELKENAYPGRGIVIGRSKDGKKAVTAYFIMGRSENS
                     RNRVFVEEGEGIRTQAF"
BASE COUNT         2588 a         1241 c         1690 g         1899 t
ORIGIN      
        1 caatggagat ggatttaaag ttgcattatt agaccttgga acaaagaata acattattcg
       61 ttctttagaa aaacgtggat gtgatgtaac agtttatcca gcagatacaa cagcagaaga
      121 aatcttagga gatcagccag acggtatcat gttatcaaac ggacctggag atccaaaaga
      181 atgtgtacag atcatcaaag agatcaagaa attatatgac agcgaggtac ctatctttgc
      241 aatctgttta ggacatcagt tgatggcact tgcaacaggt gcagacacag aaaaaatgcg
      301 ttggggacat cgtggaggaa atcatccggt gaaagactta aagagcggaa gagtttatat
      361 ttcttctcag aaccacggat atgtgatcaa ggaagaaaca attgatccag atgttgcaga
      421 agtttccttt attaatgtaa atgataaaac aatcgaagga ttagactata aaaataaaaa
      481 cattcgaaca gttcagttcc atccagaagc atgccctgga ccacaggata cagcttactt
      541 atttgatgaa tttatggata gaatgaaagg agcaaaaaat gcctaagaga gaagatataa
      601 agaaagtact tgtaatcgga tcaggaccga tcgttattgg acaggcagca gaatttgact
      661 atgcaggaac acaggcttgt agagcacttc aggaagaggg agtggaagta gtcttagtaa
      721 actcaaaccc tgcaacgatc atgacagata aagacattgc agataaagta tatatcgagc
      781 cattaacagc ttctgtcatc gaaaagatca ttgaaaaaga aaaaccagac agcatccttc
      841 caacacttgg tggacaggca gccttaaatc tggccatgga attagtagag agcggatacc
      901 ttgaaaaatc cggagtgaaa ttgatcggta catctccatc tacgatcaaa aaagcagaag
      961 accgtttaga attccagcag acaatggaaa agatcgggga accgatcgcc gcaggacagg
     1021 ttgttaaaac agtagaagat ggtgtggaat tcgctaaagt gatcggatat ccagcagtac
     1081 ttcgtccagc atatacactt ggaggaagtg gtggaggaat cgcccataac caggaagaat
     1141 tagagacgat ccttgagaat ggtcttcgtc taagccgtgt aggtgaagta ttagtagaac
     1201 gttacattgg tggatggaag gaagtcgaat acgaagtaat gcgtgatgaa aatggaacat
     1261 gtattacaat ctgtaacatg gaaaacatcg acccagttgg tgttcataca ggagacagta
     1321 tcgtagtagc tccatctcag actttaggag ataaagaata ccagatgctt cgtacatctg
     1381 cattaaacat catcacagaa ttagatgttc atggtggatg taacgtacag tacgcattga
     1441 atccagacag ctttgaatac tgcgttattg aggtaaaccc tcgtgtcagc cgttcttcag
     1501 ccttagcttc caaagcaaca ggatacccaa ttgcgaaagt aacagcaaag atcgcaatcg
     1561 gatatacatt agatgagatt gaaaatgcag taacgaagaa aacatatgca agttttgagc
     1621 caatgcttga ctactgtgtt gttaaaatgc ctagacttcc attcgataag tttattcatg
     1681 caaaacgtac tctgacaact cagatgaaag caactggaga agttatgagt atcggaacaa
     1741 acttcgaagg ggcgttaatg aaagcgatcc gttctttaga gcagcatgtg gacagcttgc
     1801 tcttcggtga ttatgataaa ttaacagacg acgagttatt aaaagaactt gaaaagatcg
     1861 atgatcttcg tatctatgtg atcgcagaag cgatccgtcg tggatttgat tacgatacga
     1921 tctttaatat tacaaagatc gatcgttggt tcttagataa gattgcagtt ttagttgaga
     1981 tggaaaagaa attagccaaa gctgataaat tagacaaaga attattacag gaagcaaaac
     2041 gcttagaatt cccagacaag gtgatcggaa gcttaacagg taagacagag cgtgagatca
     2101 aagaattaag aaatgaatat aatatccatg cagcatataa gatggtagat acatgtgcag
     2161 ccgagtttga tgcagcaaca ccttactact attcttgcta tgatggtgag aacgaagtga
     2221 aagaaacatc cgaaaagaaa aaagtcatgg tacttggttc tggaccgatc cgtatcggtc
     2281 agggtatcga atttgacttc tgttctgtac acagtgtatg ggcattaagt aaagaaggat
     2341 acgagacgat catcgtaaat aacaaccctg aaacagtttc tacagacttt gacgttgctg
     2401 ataaacttta cttcgagcca tcaacagcag aagatgtaga aagtatcgta gatcttgaaa
     2461 aaccagatgg tgcggttgta cagtttggtg gacagacagc gatcaaatta acagaagcac
     2521 tgatgaagat gggagtaccg atccttggaa caagtgctga aaatgtcgat gcagccgaag
     2581 acagagaatt attcgatgag atcttggaag aatgctgtat tccaagacca aaaggaacaa
     2641 cagtatttac atgtgaagaa gcattagaag ttgctcataa acttggatat ccagttcttg
     2701 taagaccttc ttatgtactt ggtggacagg gaatgcagat tgctgtatct gatgaagata
     2761 ttacggcatt catggaagtt atcaaccgtt acgaacagga acatccaatt ctgatcgata
     2821 aatatctggt tggtaaagaa gtcgaagtcg atgctgtttg tgatggaaca gatatcctga
     2881 tcccaggaat catggaacat gtggaacgtg ctggaatcca ttcaggagat agtatttccg
     2941 tatatccatc tattaattta acagacagga ttaaacgaat catcgnnnnn nnnnnaaatc
     3001 attacatgtt atcggactga tcaacattca gtttatcgta gcagatgatg aagtatatgt
     3061 tatcgaagta aacccaagat ccagccgtac agtgccttac atcagtaaag ttacaggtat
     3121 tccgatcgta gcattagcag cgaaagtgat cactggagct aagatcagag atcttggata
     3181 tgaaccagga cttcagaaag aatctgaata ctatgcagtg aagaaaccag tattctcttt
     3241 tgaaaaatta agaggagcag agatcagcct tgggcctgaa atgaaatcta caggtgactg
     3301 tcttggaatt tctaagaact tccatgaagc gttatacaaa gcattcttag gagctggagt
     3361 aaatcttcca aaatacaaga agatgatctt aacagtgaaa gactccgata agattgatgc
     3421 gattgatatt ggacgcagat ttgaagcatt aggatatgag attttctcaa caaagagcac
     3481 ttgcagagtc ttaaatgaag ctggagttca tgcaaaacag attaataaat tagaagaaga
     3541 atctccaaac ttgctagatc tcattttaga tgataaaatt gatttagtca ttgatacacc
     3601 atctcagggt gtaggtcgaa gcagagatgg attcctgatc cgccgttacg cgattgaaac
     3661 aggagtaaca tgccttacaa gtcttgatac ggcagatgct ctactgacta gtttggaaac
     3721 aggaaacaga gatcagctca gcgtagttga tatcgcacag atttaattaa gaagtaagaa
     3781 ggagtgatgt tatgggtcga tacacaaaac aggatgtgat agacctcgtc agagaaaatg
     3841 atgtcgaatt tatcagacta caattttctg acttgttcgg aacaatgaag aacgtcgcga
     3901 tcaccgcaag acagcttgag aaagcgttgg ataataagtg tacatttgat ggatctagtg
     3961 tagatggatt tgtccgtatt gaagaatcag atatgtattt atatccagat cttgacagct
     4021 ttgcaatctt cccgtggaat gcgggaggaa accgcgtggc aagattgatc tgtgatgtat
     4081 acggacaaga tggacttcca tttgctggag atccaagaaa tgtattaaaa cgagtgatgg
     4141 ttgagtgcca gaaaatgggt tataaattta acgtcggtcc ggaatgtgaa tttttcttat
     4201 tccacacaga tgaagaaggc cgcccaacga cggtttctca tgagaaagcc ggatattttg
     4261 atgtagctcc gttagattta ggagaaagtg ccagaagaga tatgatcttg aatcttgaag
     4321 atatgggatt tgagatcgag gcatctcatc atgaagttgc accagcacag catgagatcg
     4381 actttaagta tgacaatgcc ttaagaacgg ccgataacat cctcacattt aaattcatcg
     4441 taaaatatat tgctaaaaga catggacttt atgcaagttt tatgccaaaa ccgatcactg
     4501 gagtggacgg ttcaggaatg catcttaaca tgtctttatg gaagaatgga aagaatattt
     4561 ttgctgattc taagaataat cttggaatca gtgaggaggc ttttcacttc atggctggag
     4621 ttatggaaca tgcaaatgag atgtgcctga tcacaaatcc actggtaaac tcttataaga
     4681 gaatcgtgcc aggatatgag gcacctgtgg atgtttcatg gtcaccatcg aacagaagtc
     4741 cacttatcag agtaccagca gcccgtggaa gcggaacaag gcttgaattt agaagccctg
     4801 attctgctgc gaatccttac cttgcactgg cagtcagcct tgcagcagga ttagatggga
     4861 tcaagcgcaa attaacacca ggaaaaggtt ccatgaagaa tcagtatgag atggctgagg
     4921 gagaaaaaca agaagcaggg attaatacac tgccaagaga catgaaagaa gctcttgaga
     4981 ttttcaaaca aagcacttat atgaaagaac ttttaggaga tcatattttt aacaagtatg
     5041 ttgaagctaa gagtgctgaa tgggagtctt atcagaagtt cgtatcacaa tgggaagtcg
     5101 atcgttattt atacaagatc taatgattga tatatacatg atacgggagg tgagaagctt
     5161 ggtaaacgtc atcgttttat tcccgaaaca agaagtcgcc agaagcatta ggaatcttct
     5221 ggtaagaagt ggttttgaag tgaccgctgt atgtgcaacg ggtgcacagg tagtccagcg
     5281 tatggaaggt gttgaagaag gactagtcgt ctgcggatac aaatgttccg atatgatcta
     5341 cagtgaatta agagaatatc tttctgaaga ggtcaagatg ttgctgatcg cttctcgcca
     5401 atatttagat gactgcgtgt atccgaatgt cataggactt tcgatgccgt taaaagggta
     5461 tgaattagtc gagaaaacga gagagatcgt taccgaaatt tctgagaagc aaagacgaag
     5521 aaaacaacgt ccaaaacaac gtacgagaga agaagtccgt cttctggaag aagtcaaaga
     5581 attactgatg cagaataatc agattacaga gagtgaagcc cacgcatata tacaacaaag
     5641 aagcatgaac agtggagtgg gtcttatcga aatggcaaag atgattaaag aatcattaac
     5701 atatcaaaac taggaggaat ttatgtttaa aattaacgac aactacttga aactgcctgg
     5761 aagttattta ttttctacaa ttgcgaaaaa agtaaatgca tattccgagg caaaccctga
     5821 caaaaatatt attcgtttag gaattggaga cgtaacacag cctctggcac cagcgatcat
     5881 tgactcttta cacaaagcag tagatgaaat gggacatgca gaaacatttc acggatacgc
     5941 accagacctt ggatatgaat ttttaagaaa tgcaatcgca aagaatgatt atcaggatcg
     6001 tggagcagat atcaaagcag atgaaatctt tgtaagtgat ggagcaaaat gtgattctgg
     6061 aaatattcag gaaatctttg cacaggataa caagattgcc gtatgtgacc ctgtataccc
     6121 agtatatgta gatacaaacg ttatggcagg aagagccgga acatatgata aagcaacaga
     6181 aacatggagc gatgttatct acatgccatg tacagcagag aataacttcg caccagattt
     6241 cccaagcgaa gtgccagata tcatttacct ttgcttccct aacaatccaa ctggatcaac
     6301 aatcacaaaa gaccagttac agggatgggt agactatgca aacaaaaacg gatgcgtgat
     6361 catttatgat gcagcatacg aagcatatat ctcagaagat aacgttgcac atacaattta
     6421 tgaatgtgaa ggtgcaagaa catgtgcgat cgaattaaag agtttctcta agaacgcagg
     6481 atttacagga gttcgtttag gatatactgt tatccctaaa gatttaaaat gtggagatac
     6541 acagttacat gcgttatggg caagacgtca cggaacaaaa tataacggag ctccatacat
     6601 cgtacagaga gctggagaag cagtttactc tgaagccgga aaagctcagt taaaagaaca
     6661 ggttggatac tacatgaaca atgcaaaagt aatctttaac ggattacagg atgcaggata
     6721 cacagtatct ggtggtgtca atgcaccata catctggtta aagacaccag acaacatgac
     6781 atcttgggaa ttcttcgatc atctgttaga agatgccaac gtcgttggaa caccaggatc
     6841 aggattcgga ccaagcggag aaggatacta ccgacttaca gcgttcggta actatgagaa
     6901 tacagtcgaa gctattgaac gaatcaagag aatgtaattt ttgaatataa aataagaaaa
     6961 aggatggaat gtcagggatt gatgttccat tcttttttgt atgttaaaat aataaattat
     7021 gcggaatttc taccttatgg agttgcttgc tataagaagg attgtaaaat aaaaaagcat
     7081 aactatgtaa aaaatgatag aaataaagga cagtgttaca aaaatatata ggaaacctgc
     7141 cctttatttt ataattgtta gttttttctt gtattcaatc cattttttat atacaataga
     7201 agaaataaaa aaacaagatt ttcaaaagaa ggagatttaa gaccaatgaa gaagttagcg
     7261 cttgctcagg aattaaagga aaatgcttac ccaggacgag gaatcgtcat cggaagatcc
     7321 aaagatggga agaaagcggt cactgcatac tttatcatgg gacgcagtga gaatagccgt
     7381 aatcgtgtct ttgtagaaga aggggaagga atccgcacac aggcattt
//
LOCUS       DAOMUE010000070         7406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2621_length_7406_cov_2.213032, whole genome shotgun sequence.
ACCESSION   DAOMUE010000070 DAOMUE010000000
VERSION     DAOMUE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2621_length_7406_cov_2.213032"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLC71_04595"
     CDS             <1..521
                     /locus_tag="PLC71_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRM01955.1"
                     /translation="ILFLTNTSGGIHRGFEAGDFMMITDQIASFVPSPLMGPNIEEFG
                     ERFPDMSHIYDEELQEVIRKSADHLQIDLKEGTYIQLPGPNFESPAEIRMCKAIGADA
                     VGMSTACEAIAANHIGMKICGISCIANPAAGISKKPLTHEEVQQSADRAAPKFQKLVT
                     ECICRMGKSLKK"
     gene            533..1816
                     /locus_tag="PLC71_04600"
     CDS             533..1816
                     /locus_tag="PLC71_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRM01956.1"
                     /translation="MIRIYHARILTMQEDQEIFDGEIWISDHKIQYVGPENKEEAAKI
                     AWERQIDAKGNLIMPGFKNAHTHSAMTFLRSHADDMPLLSWLNDQVFPYEAKLTPDDI
                     YHLSKLAIMEYLTSGITANADMYLTPDTMIQASEDCGFRTTVIGAVNDFTQSVKEVEN
                     WYEKYHKEDGLTSYELGFHAEYTTKREILEGISELSKKYHAPIYTHNSESASEVAQCV
                     ERTGMTPTVYMEQLGLFEYGGGLYHCVHMSEEDLDVVKRHQISVITNPASNLKLASGI
                     APIEEMLKRNIDLAIGTDGPASNNCLDMFREMFLVTALAKYRENDASAVDAAEVLKMA
                     TTGGAKAMNLSDCDVLVKGKQADLIMIDLHQPNMQPIHNIRKNLVYSGSKQNVKMTMV
                     AGKILYEDGEFFIGTDAAHIYEKANEITQRIMSEI"
     gene            2250..2612
                     /locus_tag="PLC71_04605"
     CDS             2250..2612
                     /locus_tag="PLC71_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM01957.1"
                     /translation="MKPINYKALGAKIKEYRKKENITQEQLAEMADISLSHMSNVETA
                     SVSVSLPALKLIADALGVTIDELLVDSYSKKQKKYFYSKKMDLIMEQCETVEQEIIVN
                     TVSTLADNLLKSRKKKGV"
     gene            complement(2671..2754)
                     /locus_tag="PLC71_04610"
     tRNA            complement(2671..2754)
                     /locus_tag="PLC71_04610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2718..2720),aa:Leu,seq:cag)
     gene            complement(2760..2844)
                     /locus_tag="PLC71_04615"
     tRNA            complement(2760..2844)
                     /locus_tag="PLC71_04615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2808..2810),aa:Leu,seq:aag)
     assembly_gap    2886..2895
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3048..3953
                     /locus_tag="PLC71_04620"
     CDS             3048..3953
                     /locus_tag="PLC71_04620"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRM01958.1"
                     /translation="MLIGSHVGMKGKDMFLGSVKEALSYGANTFMVYTGAPQNTRRKK
                     IEELRIEDGTKLMKEHGMETFVVHAPYIINLANTTKPETFELAVEFLEVEVERTKAMG
                     SHTLVLHPGSHVGAGVDKGIDRIIEGLNQVMYQDMPVHIALETMAGKGSEIGREFEEL
                     KRIYDGVKYPEKLRVCFDTCHVSDSGLDLSGEGFENVIDQFDKTIGKDQIAVFHINDS
                     KNVIGAGKDRHENLGFGTIGFETLNHIVHHKDFEQVPKILETPYIKAEDSKKSYPPYK
                     YEIEMLKQEQFDPQMKEKILEDNQK"
     gene            3950..4624
                     /locus_tag="PLC71_04625"
     CDS             3950..4624
                     /locus_tag="PLC71_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM01959.1"
                     /translation="MKVTFIEHSGFMVEMEQNVLLFDYYQGEIPSFDGSKTLYVFASH
                     SHADHYDPAIWKLKEQYKDIHYILSDDIKDNEGAVVMKAHEKKEVAGIEIETLRSNDM
                     GVAFLVKVEGKTIYHAGDLNWWHWNGEPEEDNEYYKKTFQDEMKYLEGKKIDLAFMLL
                     DPRQEDKYCWGMNYFLEHTDSKVVFPMHCFEHYKINHHYLNCEDGRRWKDIVRDITGA
                     GQVFEI"
     gene            complement(4606..5091)
                     /locus_tag="PLC71_04630"
     CDS             complement(4606..5091)
                     /locus_tag="PLC71_04630"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HRM01960.1"
                     /translation="MTHVIHEIPPVYDDRSKILILGSFPSVKSREGQFFYHHKQNRFW
                     KVLAAVFEDTLPETIEEKKEFLLRHNIAIWDVIASCDIEGSSDSSIKNAVPNDFSFIL
                     KTAPIRQIYTNGGTAYKLYHKYCEKVTGFEAVKLPSTSPANASYSLERLISQWNQISN
                     T"
     gene            5215..6054
                     /locus_tag="PLC71_04635"
     CDS             5215..6054
                     /locus_tag="PLC71_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HRM01961.1"
                     /translation="MTKYFLEIFYNCIMYFIEMFLEQWIMIAFQLKLLEWIIHKKMKF
                     YKIFAASIFGSFSGCLILQLYDEDHGLKLGLMIISIVMTVKISCAFPMKKAWKYSVYL
                     LVLSLFTGGIFTMIKSQIILPWLILATASCMLVRYLYVSAVKDMNHDKESFYQVEFTI
                     LHKKLEVCAFLDTGNFLYEPVSQMPVCILEEETFLKYFHEPLFKMIEKQEAEGIRMIP
                     YQSVGREHGMMPAVMANDMKITKEDRIIEHKKAVIAISKVSLSKWGAYEMLLHPDLIK
                     NGR"
     gene            6054..6776
                     /gene="sigE"
                     /locus_tag="PLC71_04640"
     CDS             6054..6776
                     /gene="sigE"
                     /locus_tag="PLC71_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HRM01962.1"
                     /translation="MIVLKLSLVSGRRTFFGRGNEIHYIGGTEILPAPLDTAEEQKMI
                     HMLGTVNEETARSMLIERNLRLVVYIAKKFDNTGIGVEDLISIGTIGLIKAINTFNPA
                     KKIKLATYASRCIENEILMYLRRNNKTKMEVSIDEPLNVDWDGNELLLSDILGTDEDI
                     IYKDLEHEVDKKLLTRAMTILTDREKMIIELRFGINQKEGKEKTQKEVADMLGISQSY
                     ISRLEKKIIKRLKREMIKMGCV"
     gene            6880..>7406
                     /locus_tag="PLC71_04645"
     CDS             6880..>7406
                     /locus_tag="PLC71_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HRM01963.1"
                     /translation="MKMKRSEKLGMFTGLVVGVLLLLISVFMIFQTTCKVWGAEKPAN
                     ATQQGIDVSSHQGKIDWEQVKNSALADYAIIRCGYGVNQTDKDDKYWDYNSSECERLG
                     IPYGTYLYSGADTTAKAKSEAEHVVRLLQGKNLTYPIYYDMEADMLNQLSSTQIGNIA
                     KTFLNTMESYGYKNVA"
BASE COUNT         2586 a         1192 c         1665 g         1953 t
ORIGIN      
        1 agatcttgtt tttgacaaat acatccggtg ggattcatcg tggatttgaa gcgggtgact
       61 ttatgatgat cacggatcag attgcaagtt ttgtaccatc tccattaatg gggccgaata
      121 tcgaagaatt tggagaaaga tttccggata tgagtcatat ttatgatgaa gaattacaag
      181 aagtgatcag aaaaagtgcg gatcatctgc agatcgatct taaagaagga acttatatcc
      241 agcttccagg tcctaatttt gaatctccgg cagaaatcag gatgtgtaaa gccatcggtg
      301 cagatgcggt tggaatgagt actgcctgtg aggcgatcgc ggcaaatcat ataggaatga
      361 agatctgtgg aatttcgtgt attgcgaatc ctgctgccgg aatcagtaag aaaccactga
      421 cacacgaaga agttcagcaa agtgcagatc gtgcagctcc aaaattccag aaactagtaa
      481 cagaatgcat ctgtagaatg gggaagagtc tgaagaaata agaggtgatc aaatgataag
      541 aatctaccat gcaagaattt taaccatgca ggaagatcaa gagatctttg atggagagat
      601 ttggatctca gatcataaaa tacagtatgt cggaccggag aataaagaag aagcggcaaa
      661 gatcgcatgg gagagacaga ttgatgcaaa aggcaatctg atcatgcctg gatttaagaa
      721 tgcccataca cattctgcga tgacattttt aagatctcat gcagatgata tgccgctgct
      781 ttcctggtta aatgatcagg tgttcccata tgaagccaag ttaacaccgg atgatatcta
      841 tcatttatcc aaacttgcca tcatggaata tttaacaagc gggatcaccg ccaatgcgga
      901 tatgtatctg acaccagata cgatgatcca ggcatctgag gattgtggtt ttcggacaac
      961 tgttattgga gcagtgaatg attttaccca gtctgtgaaa gaagtagaga attggtatga
     1021 gaagtaccat aaagaagatg gacttaccag ttatgaactg gggttccatg cagaatacac
     1081 aaccaagaga gaaatcttag aaggcatctc agagctttct aagaagtacc atgcacctat
     1141 ctatacccac aactcagaaa gtgcctcaga agtggcacag tgcgtcgaga gaacaggtat
     1201 gacaccaacg gtatatatgg aacagctagg gctgtttgaa tatggaggag ggttgtacca
     1261 ctgtgttcat atgagcgaag aagatcttga tgtcgtaaag agacatcaga tttctgtgat
     1321 caccaaccca gcctcgaatc taaaattagc aagcgggatc gctccgatcg aggagatgtt
     1381 aaaacgaaat attgatctgg cgatcggaac ggatggacct gcaagcaata actgtcttga
     1441 tatgtttcgt gagatgtttc ttgttacagc acttgccaaa tatcgggaaa atgatgcgag
     1501 tgcagttgat gcagcagagg tattaaagat ggcaacaaca ggcggtgcaa aagcaatgaa
     1561 tctttcagac tgtgatgtgt tagtcaaagg aaagcaggca gatcttataa tgatcgacct
     1621 gcatcagcca aatatgcagc caatccacaa tatcagaaag aatctggtat acagtggaag
     1681 taagcagaat gtcaagatga caatggttgc aggaaagatt ctgtatgaag atggcgaatt
     1741 ctttattgga acagatgcgg ctcacattta tgaaaaagcg aatgagatca cgcagaggat
     1801 catgagcgag atctgaattt aatcaataca atcttctgca tgctttagtg cagaagcata
     1861 gtgttaagaa atgaaaagat gaaatgtttt caagaatata caaacaaatc atcatatcaa
     1921 aaaaaggaag cctaaatctc aggcttcctt tttcaatgga aatgggtgat atttcttttc
     1981 ccattttttc aaaagatgtt tgatcttata ttttctataa gtttgtatga tcacaaaaac
     2041 agatagaaca aaaagaacag gtgctaaaat aaatttcatg aatagatgca taaaaacctc
     2101 ctatttataa atgataacaa taaattagtt ggttttatca taacagaaat aaggccagcg
     2161 gtccattaca atagaaacta ttgccacagg cggttatttg aaaaaatgat tcataataaa
     2221 atcagagtat ataaggagtg tggagagcta tgaagcccat taattataaa gctctgggag
     2281 cgaaaattaa agaatatcgt aaaaaagaaa atataacaca ggaacaactt gccgagatgg
     2341 cagatatttc attaagtcat atgtcaaatg tggagacggc ttcagtaagc gtttcgctgc
     2401 cagcacttaa gctgatcgca gatgcattag gtgttaccat agatgaactg ttggtggaca
     2461 gttacagcaa gaaacagaaa aaatattttt acagcaagaa gatggatctg atcatggaac
     2521 aatgtgagac ggtagagcag gagattattg tgaatactgt atcaacctta gcagataatt
     2581 tattaaagag tcgaaagaaa aaaggagtct gaggactcct ttttgtagtt attgtatgaa
     2641 ataaaaaaac cgaagtcaca aacttcggta tgccgatggc gagattcgaa ctcgcacgaa
     2701 cgtaagctca ctgcgacctg aacacagcgc gtctgccaat tccgccacat cggcttaagt
     2761 gcagttgagg ggacttgaac ccctacccag ttaacccgga ctagatcctt agtctagcgc
     2821 gtatgccaat tccgccacaa ctgcgtttta gataatcgca agcgtctgtg ccgctcacag
     2881 ataatnnnnn nnnnnctata tggaacttgt gtcaataaaa aaacgaaaaa aagttcaaaa
     2941 aaattaaatt aagtatcaaa atatggattt tacagcatat tttcggtata atctgcaaca
     3001 gaatgacaca caaaaaagaa gaaaaatttt taatcggagg gaaaattatg ctgattggtt
     3061 ctcatgtagg gatgaaagga aaggacatgt ttctaggatc tgtcaaagaa gcattatctt
     3121 atggagcgaa tacctttatg gtgtacactg gtgcaccaca gaatacaaga agaaaaaaga
     3181 ttgaggaatt aagaattgaa gatggaacga aattaatgaa agaacatgga atggaaacgt
     3241 ttgttgttca tgcaccatat atcatcaatc ttgcgaatac gacgaaacct gagacatttg
     3301 agttagcagt ggaattctta gaagttgaag tagaacgtac caaagctatg ggaagtcata
     3361 cgttggtcct gcatccaggt tctcatgtcg gagctggtgt tgacaaagga atcgatcgta
     3421 ttatcgaagg attaaatcag gtcatgtatc aggatatgcc agttcatatc gcactggaga
     3481 caatggcagg aaaaggaagc gagatcgggc gtgagtttga agaactaaaa agaatctatg
     3541 atggtgtaaa atatccagag aaattaagag tctgtttcga tacttgtcat gtgagcgaca
     3601 gcggcttaga tctgtccgga gaaggatttg aaaatgtcat tgaccagttt gataagacga
     3661 tcggcaaaga ccagatcgca gtctttcata tcaatgacag taagaatgtg atcggtgcag
     3721 gaaaagaccg tcatgaaaat cttggatttg gaacgatcgg atttgagacg ttaaatcaca
     3781 tcgtacatca caaagacttt gaacaggtac ctaagatttt agaaacacca tacattaaag
     3841 cggaagacag caagaaatcc tatccgccat acaaatatga gattgagatg ttaaaacagg
     3901 aacagtttga tccgcagatg aaggaaaaga ttttggagga taaccagaaa tgaaagtaac
     3961 atttattgaa catagtggtt ttatggttga gatggaacaa aatgttctgc tgtttgatta
     4021 ctatcaggga gaaatcccgt catttgacgg cagtaagact ctctatgtct ttgcatcaca
     4081 cagccatgcc gatcattatg atccagcaat ctggaaacta aaagaacagt ataaagatat
     4141 tcattatatt ctttctgatg atatcaaaga taacgaaggt gcagtcgtta tgaaagcaca
     4201 tgaaaaaaag gaagttgccg gaatcgaaat agaaacttta agatccaatg acatgggagt
     4261 agcatttctt gtgaaagtgg aaggaaagac aatctaccat gcaggggatc ttaactggtg
     4321 gcattggaat ggagaaccag aggaagataa tgaatattac aagaagacct tccaggatga
     4381 gatgaaatat ctggaaggaa agaagatcga tcttgcattt atgctccttg atccaagaca
     4441 ggaagataag tattgctggg gaatgaatta tttcttagaa catacggaca gtaaagttgt
     4501 attcccaatg cattgctttg agcattacaa gatcaaccat cattatttaa actgtgaaga
     4561 tgggcgcaga tggaaggata tcgtcagaga tatcacaggt gcaggtcagg tatttgaaat
     4621 ctgattccac tgactgatca gccgttctaa cgaataagaa gcattcgcag ggcttgtgga
     4681 aggcagtttc acagcttcaa atccagtcac tttttcacaa tatttatgat aaagtttata
     4741 agcagtccca ccgttggtat aaatctggcg aattggggct gtttttaaaa tgaatgaaaa
     4801 atcatttggc actgcatttt taatactgct gtcgctggaa ccttcaatat cgcaggaagc
     4861 gatcacatcc cagatggcaa tgttgtgtct taataaaaac tcttttttct cctcgatcgt
     4921 ttcaggcaga gtatcctcaa agactgcagc aagaaccttc cagaaacggt tttgcttatg
     4981 atgataaaaa aactgtccct ctctggactt tacagaagga aaagaaccaa ggatcaggat
     5041 ctttgatcgg tcatcatata cgggagggat ttcatgaatc acatgagtca tttcaaaatt
     5101 tcctttctta tcgtattttt ctacaaattt caacttctat tttatcagat gatgcagaca
     5161 gaagaaagtt ttcgtcagaa actgtaaaaa cttgcagggc gttaccgcca tggtatgaca
     5221 aaatattttt tagagatttt ctataattgc attatgtatt tcatcgaaat gtttttggaa
     5281 caatggatca tgatcgcgtt ccagctaaaa ttactggaat ggatcataca taaaaagatg
     5341 aaattttata aaatatttgc cgcatccatc tttggaagtt tttcaggatg tcttatactt
     5401 cagctttatg acgaagatca tggactgaaa ttgggattga tgatcatttc cattgttatg
     5461 acggtaaaga tatcttgtgc atttccaatg aaaaaagcat ggaaatacag cgtgtatctt
     5521 ctggtgttga gtttatttac tggtggaatc tttacaatga taaaaagcca gatcattctg
     5581 ccgtggctga tccttgcgac agccagctgc atgcttgtaa ggtatttgta tgtatcagcc
     5641 gtaaaagata tgaatcacga caaagaatct ttttatcaag tggaatttac gatcctgcat
     5701 aagaaattag aggtttgtgc attcttggat acaggaaatt tcttatatga accagtcagt
     5761 caaatgccag tatgtattct ggaagaagaa acatttttga agtattttca tgagccgttg
     5821 ttcaagatga tagaaaaaca ggaagcagag ggcatccgca tgattccata tcaaagtgtt
     5881 ggaagggaac atgggatgat gccggcagtg atggcaaatg atatgaaaat cacgaaagag
     5941 gaccggataa tagaacacaa aaaggcagtg atcgccatat ccaaagtatc cctttccaaa
     6001 tggggagctt acgaaatgtt actacatccg gatctgatca aaaatgggag gtaatgattg
     6061 tgttaaagtt gagcttggta agtggaagaa ggacattttt tggaagagga aatgagattc
     6121 attatattgg gggaacggag atacttccgg caccgcttga tacagcggag gagcagaaaa
     6181 tgatccatat gttaggaact gtcaatgaag aaacggcaag aagcatgctg atcgagagaa
     6241 atctacgatt ggttgtatac atagcaaaga aatttgacaa cacgggaatc ggtgtggaag
     6301 atctgatttc gattggtacg atcggtctga tcaaagcaat caatacattt aacccagcaa
     6361 agaagatcaa acttgcgact tatgcatcca gatgtatcga aaatgaaatt ttgatgtatt
     6421 taagaagaaa taacaagaca aaaatggaag tttccatcga tgagccatta aatgtagact
     6481 gggatggaaa tgaattatta ttatccgata tccttggaac cgatgaagat atcatttaca
     6541 aggacttaga acatgaagta gataagaaac ttctgacaag agccatgacg atccttacgg
     6601 accgcgagaa aatgatcatt gaattaaggt ttgggatcaa tcaaaaagaa ggaaaagaaa
     6661 aaacacagaa agaagtcgct gatatgcttg gaatttcaca atcttacata tcaagactgg
     6721 agaaaaagat catcaaacgc ctgaaaagag agatgatcaa gatgggatgt gtataaattt
     6781 acagaaaaat taagaaaatg ttcactttct gctgaaaaaa atatgatatg ataaatgtgg
     6841 aagaaaaaat aaggaaacga tcatacttag gaggcagaga tgaaaatgaa gagaagtgaa
     6901 aaactaggaa tgtttactgg attggttgtt ggggtactct tattgctgat cagcgtgttt
     6961 atgatatttc agacaacatg caaagtctgg ggtgcagaga aaccagccaa tgcaacacag
     7021 cagggaattg atgtaagttc gcatcaaggc aagatcgatt gggaacaggt aaagaacagt
     7081 gctttggcag attatgcgat catccgatgt ggatatggtg taaatcaaac agataaagat
     7141 gataagtatt gggactacaa ttccagtgaa tgtgagagac tggggatccc ttatggaact
     7201 tacctgtatt ctggagctga tacaacagcg aaagcaaaaa gcgaagcaga acatgtcgtc
     7261 agacttttac agggaaagaa tttaacatat ccgatttatt atgatatgga agctgatatg
     7321 ttaaatcagt taagcagcac acagatcgga aatattgcaa aaacattttt gaatacgatg
     7381 gaatcttatg gatataaaaa tgtcgc
//
LOCUS       DAOMUE010000071         7299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2674_length_7299_cov_2.963556, whole genome shotgun sequence.
ACCESSION   DAOMUE010000071 DAOMUE010000000
VERSION     DAOMUE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2674_length_7299_cov_2.963556"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3309)
                     /locus_tag="PLC71_04650"
     CDS             complement(<1..3309)
                     /locus_tag="PLC71_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HRM01964.1"
                     /translation="MSLTKTKQPFLALSKICLNNWHYIDQKILSFNDGINFFTGHSGS
                     GKSTVIDAMQIVLYANTDGRGFFNKAAADDSDRNLIEYLRGMVAVGEKDEVTYLRNKN
                     FSTTIVLEFTQTESDEKECLGVVFDVDTARNDTSRLFFWHKGGILENAYRTEEHAMTI
                     SELRKYMQKNFKKEDFYFGTSNERFRKQMYDIYLGGLDMEKFPRLFKRAIPFKMNIKL
                     EDFVKEYICMEQDIHIEDMQESVMQYGRMRQRIEDTLKEAKSLEEIKESFVKFKTKKE
                     EQDYCQYRMNKLDVLKLKTDIHLLQQKIEDGEKDLKTQEENETVLEKKLEKLAKEYDA
                     LVAQIAGSGYDQIATRKKDVQELLGQLNHSVQRWEQTSKDLKKWCDLDITPNQAIWDI
                     EKFEQGDITEDDLNRLKHTLDILRKETEEERQDNASALRALKKQEKSISDELKELKKG
                     NKAYPKEVLDARYELQMKLSEKCGDFVKVNVLADLLDMKDERWHKAVEGYLGSNKLTL
                     IVEPKYVKDAMEIYRDMDQKKYWRISIADTQKIDKQDMKVEENALSEEVLAEQSYVKK
                     LIESLIGRVIKCETIDELRNCRTGITPDGMLYKNFQLKRLDPKQYTRNSYIGDNSLRH
                     RIKELEKEKDKIFDKKDPLEKEVLSAAVILDYEYLPQSAEEYLKQQETLERAKERQEE
                     YEDLENQLTKLREGALKGLEEERDQNRLKQEDCKQEINAMKEAIWATQNALKECRQQI
                     IDQNEALIRAQNELPANGEYEQQFAQEVEKTDTEDYERLKKRAARKYYDAGTKKEEEY
                     RKLVEVRTAYLREYPNRTFSAVDENNDVYDKLYKELSSDHMEMYREKAAKQAKTAMEH
                     FKDDFVYKIRSAIREAYQRRDELNRMISGLDFGKDKYQFKITRNTGADGKYYPMFMDD
                     SLNIDPSVLNTTMDDQMNLFSMEHENKYGELMNELIEIFIPPEGATGEELENAKRDMQ
                     KYSDYRTYLSFDMEQIVDGDEKLTIGLSKMIKKNSGGEGQNPLYVALLASFAQAYGIH
                     LSPKVRRNPSIRLVVLDEAFSKMDAEKVASCIDLIRNLGFQAIISATNDKIQNYLENV
                     DKTFVYANPNKKNIS"
     gene            complement(3251..3907)
                     /locus_tag="PLC71_04655"
     CDS             complement(3251..3907)
                     /locus_tag="PLC71_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HRM01965.1"
                     /translation="MIPYYEELNDEEKNAVTKVIRTLLKQTFVLERKYDKKSGRLVYN
                     KEFRTIDLHQEFLREYFKISGIELRENLHLGVFYIEGETLIGEKISRLSTIYLLILKL
                     LYDEHMAEASSNTSVYTSLGEIHEKINDFHLMRTLPSITEMRRSIALLKKYQIIEPLD
                     VLEELNDETRMMIYPCVNVVLLGDDIRRLIESFSKEEVDSEFDEDEAAISGIIEDMPE
                     "
     gene            complement(3904..5262)
                     /locus_tag="PLC71_04660"
     CDS             complement(3904..5262)
                     /locus_tag="PLC71_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HRM01966.1"
                     /translation="MKFAYDIPDSFWSLFRSMNRELYMEALLKINEEYEYNNYYLSKE
                     VCLQVLEDWNESQRIWLSPEEFESENDASQTPPNRILNWLIKTGWLKRIEDFSTLTTN
                     IIIPDYAAVFLTAFEELVNPSTDDTEIYIQNVYATLFSFWHDKKMNLAMLKTALVNTK
                     KLNKALQDMLHNMDHFFSRLLKQKSYEEVLAEHLEGYVEEIVEKKYHILKTNDNFYLY
                     KNDIKRCLREMREDIPWMEQVQKKADKEKGEDVLSIINAIERGFDDIEHRISNMDKEH
                     SKYIRATVTRMNYMLNGESDTRGLMIQLLKTIGDSKDSEEKIEKVGQSMNLSHFEVLS
                     EKSLYKKRKRRDFASQVEEEKQQEELSREDVLRLNKIHTRYQQAEIEDFIEAYMHDDH
                     MEVKDIDIRDEESFEKLILAYDYSTRRNSKYKVIERDDKLIDNGSYRYPALTFVRRKP
                     KS"
     gene            5447..5905
                     /locus_tag="PLC71_04665"
     CDS             5447..5905
                     /locus_tag="PLC71_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM01967.1"
                     /translation="MESKQNRALKEFDSLYKMIDDVYHEIALSMDLTDSAFLILYCLL
                     ELGDGCSQKDICKLYSISKQTVNSSVKSLEDKGFLVRKAGVGRDIHLFFTEFGREFSE
                     KHIGPVFDMENATFESMEPSECEQLLSLTRKYVQILKKNMKQYVLEEKEF"
     gene            5912..7243
                     /locus_tag="PLC71_04670"
     CDS             5912..7243
                     /locus_tag="PLC71_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM01968.1"
                     /translation="MKIQLSDHFTYKRLIRFVFPSIIMMICTSMYSIIDGLFISNFAG
                     KTAFAAVNLILPVAMGVGAIGFMIGSGGSAIVAKTLGEDKKEKANEYFSMLIFTALIG
                     ATILSVFGFIFIRQICMALGARGQLLEYASTYGRIMFVSQPMFMMQTIFYNFFITAEK
                     PSYSLRMSLISGVINIVFDYLFIGVMHYGVAGAAVATAMGEYVGGLVPLIYFARKNNS
                     SRLRLVKPVWRKDILLKTCLNGSSEFMTNASSSVVNMLYNTQLMRLAGADGVAAYGVI
                     MYANFIFAAIYLGYSMGSAPITSYNYGAGNHSELKNMLKKSLSLIAVTGVCLTLISEF
                     FSHPLINIFVGYDADLFAMTLHGFRLYAFVFLINGFNIWASSFFTALNNGVVSGMIAF
                     LRTLLFQIGCIMLLPILLGLNGIWMGVVVAEMLTLIVTIYFLRSRRKVYHY"
BASE COUNT         1817 a         1487 c         1245 g         2750 t
ORIGIN      
        1 ggaaatattt ttcttgtttg gattggcgta tacaaaagtc ttatctacat tttccaagta
       61 attttgaatt ttatcatttg tcgcactgat gattgcctgg aatcctagat tcctgatcag
      121 gtcaatacaa cttgcaactt tctctgcatc catcttagag aatgcttcat ctaagacaac
      181 cagacggatg cttggatttc ttcgtacttt tggtgaaaga tgaattccat aggcctgtgc
      241 aaaacttgcg agtaatgcaa catataatgg gttctgtcct tctccaccag agtttttctt
      301 gatcatctta cttaatccga tcgtcagttt ttcatcacca tccacaatct gttccatatc
      361 aaaggacaga tacgtacgat aatcagaata tttctgcata tcacgtttcg cattctcaag
      421 ttcttcaccg gttgcacctt ctggcgggat aaagatctcg atcaattcat tcatcagttc
      481 cccgtattta ttctcatgtt ccatagagaa gagattcatc tggtcatcca tcgttgtatt
      541 taagacagat ggatcgatat tcagagaatc atccataaac attggataat attttccatc
      601 ggctcctgta tttcttgtga tcttaaattg gtatttgtct ttcccaaagt cgagtccact
      661 gatcatgcgg tttaattcat ccctgcgctg ataagcttcc ctgatcgcgc ttcgaatctt
      721 gtatacaaag tcatctttga aatgttccat tgctgttttt gcctgttttg cagctttttc
      781 acgatacatt tccatgtgat cagatgataa ttccttataa agcttgtcat acacatcatt
      841 attctcatct actgctgaga atgtacggtt cggatattcc cttaaatatg cagttctgac
      901 ttccacaagc tttcgatatt cttcttcctt cttcgttccg gcatcatagt atttccttgc
      961 tgcacgtttt ttgagacgtt catagtcttc tgtgtctgtc ttctcgacct cctgtgcaaa
     1021 ctgctgctcg tactctccat ttgccggtaa ttcattctgt gcacggatca atgcttcatt
     1081 ctggtcaatg atctgctgtc tgcattcttt taatgcattc tgtgttgccc agattgcttc
     1141 tttcattgca ttgatctctt gtttgcaatc ttcctgtttt aacctgttct ggtctctttc
     1201 ctcttccaga ccttttaagg caccttctct taattttgtc agctgatttt ctaaatcttc
     1261 atattcttcc tgacgttctt tcgcacgttc cagtgtttcc tgctgtttta gatattcttc
     1321 ggctgactgt ggaagatatt catagtccaa aataacggca gcacttaaaa cttctttttc
     1381 taatggatct tttttatcaa agatcttatc cttttctttc tctaattctt tgatcctgtg
     1441 gcgtaaactg ttgtctccaa tgtaggaatt tcttgtgtac tgttttggat ctaatcgttt
     1501 taactggaaa tttttataaa gcattccatc tggtgtgata ccagtacggc aatttcttaa
     1561 ttcatcgatc gtctcacatt tgatcacacg cccgatcaag gattctatca gctttttcac
     1621 ataagactgt tctgcaagaa cttcttctga caatgcattt tcctcaacct tcatgtcctg
     1681 cttatcaatc ttttgtgtgt ctgcaatcga aattctccag tatttcttct gatccatatc
     1741 acgatagatt tccatggcat ccttgacgta ttttggttcc acgatcagtg ttaatttatt
     1801 cgatcctaaa tatccttcga ccgctttatg ccatctctca tctttcatat ctaacagatc
     1861 ggcaagcaca tttaccttta caaaatcacc acatttttcg cttaatttca tctgtaattc
     1921 atatcttgca tccagaactt cttttggata tgctttgtta ccctttttca gttctttcaa
     1981 ctcatcagaa atagatttct cctgtttctt taaggctctt agtgcagacg cattatcttg
     2041 tcgttcttcc tcagtttctt ttcttaagat atccaatgta tgttttaatc ggtttagatc
     2101 atcttctgtg atatctccct gttcaaattt ctcgatatcc cagattgcct gatttggcgt
     2161 aatatcgaga tcacaccatt tttttaagtc ttttgatgtc tgttcccaac gctgtacgga
     2221 atgatttaac tgtcctaaca actcctgaac atcctttttc cttgttgcga tctggtcgta
     2281 tccacttcct gcgatctgtg ctactaatgc atcgtattct ttggcgagtt tctctagttt
     2341 cttctcaaga actgtttcat tctcttcctg tgtctttaga tctttctctc catcttcaat
     2401 cttttgctgt aatagatgaa tatctgtttt taatttcagg acatccagtt tgttcatgcg
     2461 gtactgacaa taatcctgtt cctctttttt ggtcttgaat ttaacgaaac tttctttgat
     2521 ctcttctaag ctctttgctt cctttaatgt atcttcgatt ctctgacgca ttcttccata
     2581 ctgcatcacg ctttcctgca tatcttcaat atgaatgtcc tgctccatac agatgtattc
     2641 tttcacaaaa tcttcgagct tgatattcat tttaaatggg atcgcgcgtt taaacagtct
     2701 tgggaatttc tccatatcaa gaccgccaag atagatgtca tacatctgtt ttctaaatct
     2761 ctcgttgctt gttccaaaat agaaatcttc ttttttgaag tttttctgca tatatttgcg
     2821 gagttccgaa attgtcatcg catgttcttc cgtacggtaa gcattctcta aaatgccacc
     2881 tttgtgccag aaaaataatc tggatgtatc atttcttgct gtatctacgt caaatacaac
     2941 tcccagacat tctttttcgt cagattctgt ctgtgtaaat tcaagaacga tcgttgtgga
     3001 gaagttttta tttctcaaat aagtaacttc atctttctct ccaaccgcaa ccattcctcg
     3061 caggtattcg atcagatttc gatcggaatc gtctgctgcc gccttattaa agaatccacg
     3121 accatctgta ttcgcatata aaacgatctg catcgcatcg atcactgttg actttccact
     3181 tccagaatgt ccggtaaaaa aattgattcc atcgttgaaa cttaatattt tctgatctat
     3241 ataatgccaa ttattcaggc atatcttcga taatgccaga aatggctgct tcgtcttcgt
     3301 caaactcact gtctacttcc tccttgctga aactttcgat caatctgcgg atgtcatctc
     3361 ctagtaacac gacattcacg catggataga tcatcattct tgtctcgtca tttaactctt
     3421 ctaatacatc gaggggttcg atgatctgat atttctttaa taatgcgatg gaacgacgca
     3481 tctcagtgat ggatggcaag gttctcatca gatggaaatc attgatcttt tcatggattt
     3541 ctcctaatga tgtatacaca cttgtattcg atgaagcctc tgccatatgt tcatcgtaca
     3601 ataattttaa gatcagaaga taaatggttg ataatctgga aatcttctct ccgatcaaag
     3661 tttctccttc aatataaaag actcccagat gcagattttc tcttaattcg attcctgaaa
     3721 tcttaaaata ttctcttaaa aattcctgat gcaggtcaat ggttctaaat tctttattgt
     3781 atacaagtct tccgctcttt ttatcatatt ttctttctaa tacaaatgtc tgttttaata
     3841 acgtacggat cactttcgtg accgcatttt tttcttcgtc attcaattct tcataatacg
     3901 gtatcatgat ttcggtttcc tccttacaaa agttaatgcc ggatagcggt aacttccatt
     3961 atcgatcaat ttatcgtctc tctcgatcac tttatattta ctgtttcttc tcgtactgta
     4021 atcgtaggca aggattaatt tctcaaagga ctcttcatcc ctgatatcaa tatccttaac
     4081 ttccatatgg tcatcatgca tgtaagcttc aatgaaatct tcaatctcag cctgttggta
     4141 tcttgtatga atcttattta atcttaggac atcttctctt gataattctt cctgctgctt
     4201 ttcttcttct acctgacttg caaaatctct tcgttttctc ttcttataca aagacttctc
     4261 tgaaagcact tcaaaatgag ataagttcat gctctgaccg actttctcaa tcttttcctc
     4321 gctgtcctta gaatccccga tcgtctttaa cagctggatc atcagacctc ttgtgtcgga
     4381 ttctccattc agcatgtaat tcatacgtgt tacagttgcc ctgatatatt tggaatgttc
     4441 tttatccata ttggaaattc gatgttctat gtcatcaaat ccacgctcga tcgcattaat
     4501 gatacttaag acatcttcac ctttttcttt atctgctttc ttctgtacct gttccatcca
     4561 tggaatatct tctctcattt ctcttaaaca tcgtttgata tcatttttat ataaatagaa
     4621 gttatcattt gtctttaaga tatgatattt cttctcaacg atctcctcga cgtaaccttc
     4681 caaatgttct gccagcactt cttcatatga tttctgtttt agcagtcggc taaagaaatg
     4741 gtccatgttg tgtaacatat cctgcaaggc tttatttaat ttctttgtat taacaagcgc
     4801 tgtttttaac atggctaagt tcatcttctt atcatgccag aatgaaaaca gtgttgcgta
     4861 tacattctgg atatagatct ctgtgtcatc cgttgatggg ttgactaatt cttcaaatgc
     4921 tgttaaaaat acggctgcgt aatcaggaat gatgatattg gtggttaatg ttgagaaatc
     4981 ttcgatcctc tttaaccagc ctgtcttgat cagccagttt aagatcctgt ttggcggtgt
     5041 ctggcttgca tcattttctg attcaaattc ttcaggagat aaccagatcc gctggctttc
     5101 gttccagtct tctaatacct gaaggcatac ttctttactt aaataataat tgttatattc
     5161 atattcttcg ttgatcttca gcagggcttc catataaagt tctctgttca tcgaacgaaa
     5221 taaactccag aatgaatctg ggatatcata ggcaaatttc ataggtaact tccttctttg
     5281 gttgtacttt attctctgac tctttatttt aactgattcg aatgacctcg taaagtgatt
     5341 tcctatgaaa taataattga cacaagtccg aaatcagact ataattatcg taatctaata
     5401 gtccgatttc ggacttaatg taattgttat aagaaaggaa gtcactatgg aatcgaaaca
     5461 aaatcgcgcc ttaaaggaat ttgattcgct ctataagatg atcgatgatg tctaccatga
     5521 gatcgctctt tccatggatc ttacagattc ggcatttctc attttatatt gtctgttaga
     5581 acttggagat ggttgctctc agaaagatat ctgcaaactt tactccatct caaaacaaac
     5641 ggtaaattct tccgtaaaat ctctggaaga caaaggattt ttggtccgta aagccggtgt
     5701 cggaagggat atccatttat tttttacaga gtttggacga gaattttctg aaaaacatat
     5761 cggtcctgtc tttgatatgg aaaatgctac atttgaatct atggagcctt cggagtgtga
     5821 acagctgctt tctcttacga gaaaatatgt tcagatttta aagaaaaata tgaaacaata
     5881 tgtcttagag gaaaaggagt tttagtctat catgaagatt caattatcgg atcatttcac
     5941 ttacaaacgt ctgatccgtt ttgtatttcc atcaatcatt atgatgatct gtacttcgat
     6001 gtacagtatt attgatggat tatttatttc caattttgca ggaaaaacag catttgctgc
     6061 ggtcaatctg atccttcctg ttgcgatggg tgttggagcg atcggattta tgatcggttc
     6121 tggtggaagt gcgatcgttg caaaaacgct tggtgaagat aaaaaagaaa aagcaaatga
     6181 atatttttct atgctgatct ttactgcttt gatcggtgct acaattttat ctgtctttgg
     6241 atttattttt ataaggcaga tctgtatggc acttggggcg cgtggacagc tgcttgaata
     6301 tgcgtctact tatggacgta ttatgtttgt atcacagcca atgtttatga tgcagacgat
     6361 cttttataac ttctttatta cagctgagaa accttcttac agtcttcgta tgtctttgat
     6421 ctctggcgtg atcaatattg tgtttgacta tctctttatc ggtgtcatgc attatggtgt
     6481 tgccggggct gcggttgcaa ctgccatggg tgagtatgtt ggcgggcttg taccgttgat
     6541 ctattttgca cgaaagaata acagcagccg tcttcgtctt gtgaaacctg tctggagaaa
     6601 agatatttta ttaaagacct gtttaaatgg ttcttcggaa tttatgacaa atgcttcttc
     6661 ttccgttgta aatatgcttt ataatacaca gttaatgcgc cttgctggcg cagatggtgt
     6721 tgctgcttat ggtgtgatca tgtatgcgaa ctttatcttt gctgcgatct atcttggata
     6781 ttctatggga agcgctccga tcaccagtta taattatggg gctggaaatc attcggaatt
     6841 gaagaatatg ttgaagaaga gtttgtcttt aattgctgta actggggttt gtctgacttt
     6901 gatctctgag tttttttccc atccgctgat caatatcttt gttggatatg atgctgatct
     6961 gtttgctatg acgttacatg gattccggtt gtatgcattt gtgtttttga tcaatggatt
     7021 taatatctgg gcttcttcgt tctttacagc tttgaataat ggggttgttt ctgggatgat
     7081 tgcattcctt cgaactttgt tgttccagat tgggtgtatt atgctgctgc caattttgct
     7141 tggattgaat ggaatctgga tgggagttgt tgtggcggag atgttgactt taattgttac
     7201 gatttatttt ttacggagta gaaggaaagt ttatcactac tagattttgt aatagtgttt
     7261 catgtatgag atagaacgct attcaaatca ctaaagggt
//
LOCUS       DAOMUE010000072         7254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2707_length_7254_cov_2.950965, whole genome shotgun sequence.
ACCESSION   DAOMUE010000072 DAOMUE010000000
VERSION     DAOMUE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2707_length_7254_cov_2.950965"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1159
                     /locus_tag="PLC71_04675"
     CDS             101..1159
                     /locus_tag="PLC71_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRM01969.1"
                     /translation="MNIEEFLTLAAKEEASDLFIVAGLPLTMKVNGVMRRINEEKMMP
                     QDTEKIIREIYEKALDRDIDQLLKTGDDDFSFAIPGLSRFRVSAYKQRGSLAAVIRVI
                     AFRLPDYKQLRIPEQVMKLSELNKGLVLVTGPAGSGKSTTLACMIEEINETKEDHIIT
                     LEDPLEFLHQHKKSIVSQREVNMDTVNYVTSLRAALRQSPDVILLGEMRDYETIQVVM
                     TAAETGHLVFSTLHTIGAANTIERIIDVFPPNQQRQIMIQLASVLQAVISQQLIPTMD
                     GTLIPVFEIMEVTPAIRNMIRENKVHQIDGLIYSSTGSGMISMDQSLINLYKEGQISK
                     ETAILYASNPEMIIKRIR"
     gene            1321..2259
                     /locus_tag="PLC71_04680"
     CDS             1321..2259
                     /locus_tag="PLC71_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01970.1"
                     /translation="MKKKVMVGLVSVVVAVALVFGGVTFYNNRQQEKFEKQMASYESV
                     DTMQHPSEATTSIDGIQVPLGKKPKVTTKKTTKKSTRIQKLKKKASRSKITTRRTTKR
                     RTTTSQNHSRRKVVNTKVVKTQKDYDKKGSKKRTIKTVVQTTIKTTTVELSQMSGVSG
                     TTLRTLGSQADGKILNAFEDLKFKMVIDKNAEATGVFSVKSHKIALQSARSSVLLHEL
                     GHFANFLAGDKVGTSQWKSIYNAEKNKYDGYNKAYAIKSASEYFAESYKDYKEHPSAL
                     RSKRPRTYQFVKSTIDGITDSDVQNIKDTYGEYWGL"
     gene            2385..3182
                     /locus_tag="PLC71_04685"
     CDS             2385..3182
                     /locus_tag="PLC71_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HRM01971.1"
                     /translation="MLKIKEYVKAESLEQAYELNQKKTNCVIGGMLWLKMVTRNVQKA
                     IDLSGLGLDQIEENDEEFSIGAMVTLRQIELHEGLNTWSQGAVKESVTHIVGVQFRNL
                     ATVGGSIFGKFGFSDVLSCFLAFDSYVELHHEGIIPLDQFAANKYPNDILVRLIIKKH
                     PQESVYLSHRNTKTDFPVLTCAVSLGEKEAYAVVGARPSRACRVRLSDQWMEQIKDDE
                     GRKKLADEVTGQMNFGSNMRGSAAYREQLSKVLLRRGFEQLEERREK"
     gene            3185..3658
                     /locus_tag="PLC71_04690"
     CDS             3185..3658
                     /locus_tag="PLC71_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRM01972.1"
                     /translation="MEITFTLNGKKVTPQIDADTLLIDLVRDLGCYSVKRGCETSNCG
                     LCTVFMDDQPILSCSILAARVDGHKIDTLEGLQEEAAEFGAFIANQGAEQCGFCNPGM
                     IMNAIALFREIPDPSEEEIKEYLAGNLCRCSGYEGQLRGILDFLEYKKNKDGGAE"
     gene            3655..5940
                     /locus_tag="PLC71_04695"
     CDS             3655..5940
                     /locus_tag="PLC71_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRM01973.1"
                     /translation="MKVVNQPIMKKDAMALVTGKPVFTNDKAPKECLIVKLLRSPYAN
                     AMIKSINTQFAMKVPGIEAIYTWEDVPQERFTMAGQTYPELSPYDRQILDQHVRYVGD
                     PVAIVAGENEKCVDQAIKMLRVEYEVLPANLDPRKAMDKDTPLVHPEDNWKALCNIGA
                     DNKKNLCATEETHEGDVDAVLADCDVVVEHTYHTKANQQAMMETFRTYCFIDEYGRLN
                     VVSSTQIVFHVRRILSNALCIPKSKIRVSKPRIGGGFGAKQSSISEVYPAFVTWMTKK
                     PSKIIFSREESQIASSPRHEMQVTVRVGANKNGKIRAIDVYTLSNTGAYGEHGPTTVG
                     LSGHKSIPLYRDLEAHRFAYDVVYTNRMSAGAYRGYGATQGVFALESAVSELAAKIGM
                     DPTKIREMNMVREGDVMPAYYGETANSCALDRCLARAKEMIKWDEKYPCKDMGNGKVR
                     SVGLSMAMQGSGISGVDVGSATIKLNDDGLYTLSIAAADMGTGCDTILAQMAAECLEC
                     SIDDIMVSGADTDTSPYDSGSYASSSTYVTGKAVERACEKLKGHIFNKAAEMMEVADT
                     DTLEFDGKKVRDAESGKEVSLVDIATASMCNNNETFQVTETNSSPVSPPPFMVGMVEI
                     ELDKETGHIEILDYVAVVDCGTPVNPNLARVQVEGGLGQGIGMALYEGVDYTPNGYVK
                     QNSFMQYKVPARVDVGKLRVEFESSYEPTGPFGAKSIGEIVINTPSPALAHAVFNATG
                     LWIRELPITSEKIVMGLLEKE"
     gene            5947..6561
                     /locus_tag="PLC71_04700"
     CDS             5947..6561
                     /locus_tag="PLC71_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRM01974.1"
                     /translation="MDKIKAAVIYLASGNSKRFGQENKLLQMIGTKPMYRYGLDRLID
                     LCKDHEGFEIIVVTQYQEIMKQTQYDPITTVFCPESKYGISWSIKAGIKAAKDAEFYT
                     FFVADQPNMKRETIERFLSFMADEFCELGCVRTEENLGNPVWFSKKYKRELLSLTKDQ
                     GGKKILKKHIENARFFKVLEETELCDIDYPKEMQAMLAKRGKSS"
     gene            6601..7176
                     /locus_tag="PLC71_04705"
     CDS             6601..7176
                     /locus_tag="PLC71_04705"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HRM01975.1"
                     /translation="MELLKERIRKDGVVKEGNVLKVDRFLNHQMDVKLFREIGKEFKK
                     RFEGEDINKILTIEASGIGIACVVAEIFDVPVVFAKKTQTKNIAGDVYTTKVESFTHG
                     RVYDIIVSKEFLGEGDHVLLIDDFLANGKALEGLAKLVKDSGAELVGAGVVIEKGFQP
                     GGDFLRKQTHLESLAIVDAMDEKTGEITFRE"
BASE COUNT         2538 a         1174 c         1735 g         1807 t
ORIGIN      
        1 atctgtgtta tgaagtgatc aaatggaaaa aagaagctgt tggggcatgg aagaaggatg
       61 atttccttcc ggtttataga aataagtaaa aggggccagc atgaatattg aagaattttt
      121 aacattagca gcaaaagagg aagcctcaga tctgtttatt gtagcagggc ttccattgac
      181 gatgaaagta aatggagtga tgcgtcgcat caatgaagaa aaaatgatgc cgcaagatac
      241 ggaaaaaata atccgggaga tttatgagaa agcgttagac agagacatag atcaactgtt
      301 gaagacaggg gatgatgatt tctcatttgc aattcctgga ttgtcaagat ttcgtgtcag
      361 tgcatataaa cagagaggat ccttagcggc agtgatccgt gtgatcgcat tccgcctgcc
      421 agactataag caattaagaa taccagagca ggtaatgaaa ttaagtgaat taaacaaagg
      481 acttgtgctt gtaacaggac cagcgggaag tggaaaatcc acgacacttg catgcatgat
      541 agaagaaatc aatgaaacaa aagaagatca catcatcaca ttggaagatc ctttggaatt
      601 tttacatcaa cataagaaaa gtatcgtcag tcagagagaa gtaaacatgg atactgtaaa
      661 ttatgttact tccttaagag ccgcattaag acaaagtccg gatgtgatat tgcttgggga
      721 aatgagagat tatgaaacga ttcaggtagt tatgacagct gcagaaacag ggcatctggt
      781 attttcaaca cttcatacta tcggggcagc gaatacgatc gaacgtatca tagatgtatt
      841 tccaccaaat cagcaacgtc agatcatgat tcagctggct tctgtattac aggcagtgat
      901 ctcccagcag ctgataccaa cgatggatgg aacattgatc cctgtgtttg agatcatgga
      961 agtgaccccg gcgatccgca atatgatccg tgaaaataaa gtgcatcaaa tagatggttt
     1021 gatctattcc tctacaggga gcggaatgat atctatggac cagtcattga tcaatctata
     1081 taaagaagga cagatcagca aagaaactgc gattttatat gcatccaatc cagaaatgat
     1141 aataaaaagg atccgctaag aaatatggtc agaaacaggc tgaaatccat cggaatttat
     1201 tgacaaaaaa gtgcgaatat tgtacgataa aaatatatta tagggtgagt gtctctcaca
     1261 tggagattga gagataaaag caaagagtat agatattaat attaagaagg ggcgagagtt
     1321 atgaagaaga aagtcatggt cggattggtg tccgtggtag tagctgttgc attggttttt
     1381 ggtggagtca cattttacaa taaccgtcag caggagaagt ttgagaagca gatggcttcc
     1441 tatgaaagcg ttgatactat gcagcatcca agcgaagcaa caacatcaat tgatgggatt
     1501 caagtaccgc ttggcaagaa accgaaggta acgacgaaga aaacaacaaa gaaaagcaca
     1561 agaatccaga agcttaagaa gaaagcaagc agaagcaaaa taacaacaag aagaacaaca
     1621 aagagaagaa caacaacatc tcagaatcat tccagacgta aggttgtgaa tacaaaagtt
     1681 gtcaaaacac agaaggatta tgacaagaag ggttctaaga agagaacgat caagacagtc
     1741 gttcagacaa cgatcaagac aacaacggtt gaattgagtc agatgtctgg tgtttctgga
     1801 acaacattaa gaacacttgg ttctcaggca gatggtaaga ttttgaatgc atttgaagat
     1861 ctgaagttta agatggtgat tgataaaaat gcggaagcga caggtgtatt tagcgttaag
     1921 agccataaga tcgcacttca gagtgcgaga tcttctgtat tacttcacga attaggacat
     1981 tttgctaatt tccttgcagg agataaagtt ggaacgagtc aatggaagtc aatttacaat
     2041 gctgagaaga ataaatatga tggatataac aaagcatatg cgatcaaatc agcatctgag
     2101 tattttgcag aatcttacaa ggattataag gagcatccat ccgcattaag atcaaagaga
     2161 ccaagaactt accagtttgt taagagtacg attgatggaa ttacagacag cgatgtacag
     2221 aacatcaaag atacctatgg agaatactgg ggattatagg acgatacctg aaggagtact
     2281 ttctcagaaa gtactctttt ttttgccaat tgcctgtgtt gctctgtaga atgtgaaaga
     2341 atgtataaac aagggatatt ttaaaaaatt aaaggagaaa taagatgtta aagatcaagg
     2401 aatacgtcaa agcagaaagt cttgagcaag cttatgagct gaatcagaag aagaccaact
     2461 gcgtgatcgg tggcatgttg tggcttaaga tggtcacaag aaatgtacaa aaagcaattg
     2521 atctgtcggg tcttggactt gatcagatcg aagaaaatga tgaggaattt tcaatcggag
     2581 caatggtaac actaagacag atcgaacttc atgaaggatt aaatacatgg agtcagggag
     2641 cggtaaaaga aagtgtgaca cacattgtcg gagtgcagtt ccgtaatctt gcgacagtag
     2701 gtggaagtat ttttggaaag ttcggttttt ctgatgtatt aagctgtttt ctggcgtttg
     2761 acagttatgt ggagttacat catgaaggaa tcattccatt agatcaattt gcagcaaata
     2821 aatatccaaa tgatatttta gtgcgtctga tcattaagaa gcatccgcag gaaagtgttt
     2881 atctttctca tagaaataca aagacagatt ttccagtttt gacctgcgct gtttcattag
     2941 gtgagaaaga ggcatatgcg gttgtcggtg caagaccatc cagagcttgc agagtcagat
     3001 tatcagatca gtggatggaa cagatcaaag atgatgaagg aagaaaaaaa ctcgcagatg
     3061 aagtgacagg tcagatgaat tttggaagca acatgagagg aagtgctgct taccgtgaac
     3121 agttatcaaa agtcttatta agaagaggct ttgaacagtt agaagaaagg agagaaaaat
     3181 aaacatggag attacattta cattaaatgg aaagaaagta accccacaga ttgatgcaga
     3241 cactctcctg atcgatctcg tcagagatct tggttgttac agtgtgaaac gtggatgcga
     3301 gacatcaaat tgcggattat gtacggtatt tatggatgac cagccaatct tatcatgttc
     3361 cattcttgca gcaagagttg atggacataa gatcgatacc ttagaaggat tgcaggaaga
     3421 agcagctgag tttggagctt ttattgcgaa ccaaggggct gaacagtgcg gattttgtaa
     3481 cccaggaatg atcatgaatg cgattgcatt atttagagag attccagatc cgagtgaaga
     3541 agagatcaaa gaataccttg cggggaatct ttgcagatgt tctggatatg aaggacagct
     3601 tcgaggaatc ttagactttt tggaatataa gaaaaataaa gatggaggtg ccgaatgaaa
     3661 gtagttaacc agccgattat gaaaaaagac gctatggcac ttgtgacagg aaaacctgta
     3721 tttacaaacg ataaggcacc aaaagaatgt ctgatcgtga aattacttcg aagtccatat
     3781 gcgaacgcaa tgatcaaatc gatcaacact cagtttgcta tgaaagtgcc agggattgag
     3841 gcgatttata catgggaaga tgtgccacag gaacgtttta cgatggcagg acagacatat
     3901 ccggaattaa gcccatatga cagacagatt ctggatcagc atgtccgtta tgttggagat
     3961 cccgttgcaa tcgttgctgg ggagaatgaa aagtgcgttg atcaggcgat caagatgtta
     4021 cgtgtggaat acgaagtact gccagcaaac ttagatccac gtaaggcaat ggataaagat
     4081 acaccgctag tgcatccaga agataactgg aaagcattat gtaatattgg tgcagataat
     4141 aagaaaaacc tgtgtgcaac agaagaaaca cacgaaggag atgtcgatgc ggtattagca
     4201 gattgcgacg tggttgtaga acatacatat catacaaaag caaatcagca ggcaatgatg
     4261 gagacattca gaacttattg ttttattgac gaatatggaa gattgaacgt tgttagttcc
     4321 acacagatcg tattccatgt acgaaggatc ttatccaatg cattatgcat tccaaaatca
     4381 aagattcgtg tatcaaaacc aagaattggt ggaggatttg gagcaaaaca gagttctatc
     4441 tccgaagtat atccagcatt tgtcacatgg atgacaaaga aaccatccaa gatcattttt
     4501 tcaagagaag agtctcagat cgcatcttca ccaagacatg agatgcaggt aacagttcgt
     4561 gtcggagcaa acaagaatgg aaagatccgt gcgatcgatg tttatacatt atccaataca
     4621 ggagcctatg gagaacatgg tccaacaacg gtagggttat ctgggcataa atcaattccg
     4681 ttatatcgag atctggaagc acatcgtttt gcatatgatg ttgtgtatac aaatcgtatg
     4741 tcagccgggg cttatcgtgg atatggtgcg acacagggtg tgtttgcgct ggaatcagca
     4801 gtcagtgaac ttgcagctaa gataggaatg gatccaacga agatccgtga gatgaacatg
     4861 gtccgtgaag gcgatgtgat gccagcatat tatggagaaa cagcaaacag ctgtgctttg
     4921 gatcgatgcc ttgcccgtgc aaaagagatg atcaaatggg atgaaaaata tccatgcaaa
     4981 gatatgggaa atggaaaagt tcgaagtgtc ggactgtcaa tggcgatgca gggctccggt
     5041 atttctggcg ttgacgttgg ttctgcaacg atcaaattaa atgacgatgg attgtataca
     5101 ctttctattg cagcagcgga tatgggaact ggatgtgata cgatcttggc ccagatggcg
     5161 gcagaatgtc tggaatgttc tattgatgat atcatggtca gcggagcaga tacagataca
     5221 tcaccatacg attctggatc ttacgcatca agttctacct acgtcacagg aaaagcagtg
     5281 gaacgtgcct gcgaaaaatt aaagggacat attttcaata aagcagctga gatgatggaa
     5341 gttgcagata cagatactct ggaatttgac ggaaagaaag tcagagatgc agagagcggc
     5401 aaagaagttt cattagtgga catcgcaaca gcatctatgt gcaacaataa tgaaactttc
     5461 caggtgacag agacaaacag ttctccggta tccccaccac catttatggt aggaatggtt
     5521 gagattgagc ttgataaaga gacaggacat attgaaatct tagattatgt cgcagtcgta
     5581 gattgtggaa caccagtcaa tccaaatctt gcaagagttc aggtagaagg cgggctcggt
     5641 cagggaatcg gaatggcatt gtatgagggt gttgattaca caccaaatgg atatgtaaaa
     5701 caaaattctt tcatgcagta caaagttcca gcaagagtcg atgttggtaa gcttcgtgta
     5761 gaattcgaga gcagttatga gccaacagga ccatttggag caaaatctat cggagagatc
     5821 gttatcaaca caccatctcc agcattagcc catgccgtat ttaatgcaac cggattatgg
     5881 atccgagaat tgccgatcac cagtgaaaag atcgttatgg gattattgga gaaagaataa
     5941 tataacatgg ataaaataaa agcagcggtc atttatcttg catcaggaaa cagcaaacgt
     6001 ttcggtcagg agaataaatt attgcaaatg atcggaacca agccaatgta tcgttatgga
     6061 ttggaccgat tgatcgatct atgcaaagat catgaagggt ttgagatcat tgttgtcaca
     6121 cagtatcagg aaattatgaa gcaaacacaa tacgacccaa taacgacagt gttttgtcca
     6181 gaaagtaaat atgggatttc atggtcgatc aaagcaggga tcaaagcagc gaaagatgca
     6241 gaattctata catttttcgt agcagaccag cccaatatga aaagagagac aatcgaaaga
     6301 tttctctctt ttatggcaga tgaattttgt gaactcggct gtgtcagaac agaagaaaac
     6361 cttgggaatc ctgtatggtt ctcaaagaaa tataaacgag aattgttatc gcttacaaaa
     6421 gatcaaggtg gcaagaaaat tctcaaaaaa cacatagaaa atgccagatt cttcaaggtg
     6481 ttggaagaaa ctgaactttg cgatatagat tatccaaaag aaatgcaagc aatgcttgca
     6541 aagcgaggca aaagtagtta aaatattatt taaaatacaa aagagcgtgg aggccaagtg
     6601 atggaattat taaaagagag aattcgaaaa gatggagtcg tgaaagaagg aaatgtcctc
     6661 aaagtagacc gctttttgaa tcatcagatg gatgtaaaat tattccgtga gatcggtaaa
     6721 gaatttaaga aacgttttga aggcgaagat atcaataaga tccttacgat cgaagcatca
     6781 ggaatcggaa tcgcctgtgt tgtggcagag atttttgatg taccagtcgt atttgcaaag
     6841 aaaacacaga caaagaacat cgcgggagat gtatatacaa caaaggtaga atcattcaca
     6901 catggaagag tctacgacat cattgtatcc aaagaattct taggagaagg agatcatgta
     6961 ttgctgatcg acgatttcct tgccaacggt aaagccttag aaggattagc gaaattagtc
     7021 aaagattccg gagcagaact ggtcggagca ggagttgtga tcgagaaagg attccagcca
     7081 ggaggagatt tcttaagaaa acagacacac ttagaatccc ttgcgatcgt agatgctatg
     7141 gacgagaaaa caggagaaat cacattccgt gaataatgtc tttgacgttc ttgtaggtag
     7201 tcttgcatgt atgtgacaga aataacagaa aaacaaatat ttaaaagcat caag
//
LOCUS       DAOMUE010000073         7206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2736_length_7206_cov_2.325409, whole genome shotgun sequence.
ACCESSION   DAOMUE010000073 DAOMUE010000000
VERSION     DAOMUE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2736_length_7206_cov_2.325409"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PLC71_04710"
     CDS             <1..951
                     /locus_tag="PLC71_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM01976.1"
                     /translation="KRETNTMPQWAGSSWYFLRYIDNKNNEELVSREKADKYLPVDMY
                     IGGVEHAVLHLLYSRFYTKFLCDIGVIDFDEPFHKLFNQGMITGKNGIKMSKSKGNVV
                     SPDDLVRDYGCDSLRMYELFVGPPELDAEWDESGIDGVHRFLTRFYKLIMDQKDKGVE
                     ADKELLKVRHKLIYDITTRLNNFSLNTVVSGFMEYTNTLTAMAKKSGVDKETLETAIV
                     LLAPFAPHIAEELWEAMGHEDSVFAQTWPTYDEEAMKEDEVEIVVQINGKVKGKLVIG
                     AEEDKDSVLAKAKEVLGDKLNGNIVKEIYVPGRIVNIVQK"
     gene            1044..>1164
                     /locus_tag="PLC71_04715"
     CDS             1044..>1164
                     /locus_tag="PLC71_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HRM01977.1"
                     /translation="MYTFDSRIRYSEIDRSGRLSIPAVVDYFQDCSAFQSEELG"
     assembly_gap    1165..1174
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1175..1751
                     /locus_tag="PLC71_04720"
     CDS             <1175..1751
                     /locus_tag="PLC71_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRM01978.1"
                     /translation="VEYLANKKRTWILNSWQIVLERRPEECEKITVGTWGSGFDKFHA
                     TRNFIMKTTQGERLAYANSIWVYINTETGMPVRPTKEEIDVYKLEAPLEMEYEPRKIK
                     LSREWEEKEPVKVQRSWIDSNDHVNNSWYVKTAFEQLPQDLEIRQLRVEYKKQAYEGD
                     ILYPRYAREEQRTLVALCDEKQKPYAVIECR"
     gene            1775..2728
                     /locus_tag="PLC71_04725"
     CDS             1775..2728
                     /locus_tag="PLC71_04725"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRM01979.1"
                     /translation="MKQTKIVILGAGHVGTHCALSLMFRGLANEIVLIDTDEEKAKSQ
                     ALDLDDMGACLPAKVVIRSGSYEDLDDADILVNAIGRSRKEGETRLDMFGDSMERLKD
                     IIPKIQDTKFRGILISITNPADVVGECLRKALGIERFRCFSTGTSLDSLRIKRILEEK
                     TGYHRNSIEAFCMGEHGDSQIVPLSRVSVGGKPFAKLQKEYPDTLGKITIEDLQDEVR
                     QAGMTVIIGKKSTEFGIGIALSGIVKSIVYDEKRIWPLSVHLDGEYGQKDVAAGVPAV
                     IGADGIEEIVEMDLTEEEKSQFTHSCDVIRGYLKKAKEMLS"
     gene            complement(2736..2999)
                     /locus_tag="PLC71_04730"
     CDS             complement(2736..2999)
                     /locus_tag="PLC71_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01980.1"
                     /translation="MSDDPISFDHLSSLDPKKLEIIKFVISSCHGKPLELILPDLMAA
                     SSRLSEQGLSFTNEEISMIIDGLKESMSPEEQQRIDMIRPLIM"
     gene            complement(3011..3496)
                     /locus_tag="PLC71_04735"
     CDS             complement(3011..3496)
                     /locus_tag="PLC71_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01981.1"
                     /translation="MEQNQTSWENFFRNDSSNMLEDALPYLPPNLKKVAAIYIKLTEL
                     SKISSEFDNEQTLSACGFDQDPASMELLLNALKLRAPKETAMQIEQILQMMQMFEVYQ
                     NYQRFVDTTNHSSPSSSDSQNNDLFSKLMPMMMAKDSPCTNTAKPNSDFMNQLNQILK
                     K"
     gene            3557..3766
                     /locus_tag="PLC71_04740"
     CDS             3557..3766
                     /locus_tag="PLC71_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01982.1"
                     /translation="MNEILVEEENTVYELDPECLRHKEEQRQLEEKRKQEKQENDNYR
                     MQKRPEKRNQNNMWIICWLLLVICQ"
     gene            complement(3819..4055)
                     /locus_tag="PLC71_04745"
     CDS             complement(3819..4055)
                     /locus_tag="PLC71_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorion class high-cysteine HCB protein 13"
                     /protein_id="HRM01983.1"
                     /translation="MSDLAATGCGGSCGSSCFGGNNSCLLLLILLCCCGGGNNDGCGC
                     GNGCDSLIWIILILCCCGNGSFGCGNNNGCGCGC"
     gene            4198..5025
                     /gene="thiM"
                     /locus_tag="PLC71_04750"
     CDS             4198..5025
                     /gene="thiM"
                     /locus_tag="PLC71_04750"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HRM01984.1"
                     /translation="MKRIDFERIFDNIRRNQTMVHCITNYVTINDVANMILAIGASPI
                     MADDWMEVREITSMCDSLVINMGTLKQNTVRSMLLAGKEANQRGHLVVFDPVGVGASR
                     FRKETAAKLLKQIHFNVIRGNISEIKTLYEGSDDGYGVDAKKDDAVTEDNLEYVIQMA
                     KNMAKKTKAVIVITGKTDLVTDGQQIYLIDNGVLDMSRITGTGCMLDGVIAGFIGANP
                     DQILEAVTTAVSAMGICGEYAKEKAEGTGTLKVHLMDAMSNMNAEWMERSSQIESKC"
     gene            4979..>5292
                     /locus_tag="PLC71_04755"
     CDS             4979..>5292
                     /locus_tag="PLC71_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRM01985.1"
                     /translation="MQNGWKGAVKLRVNADAMTLYAVTDRTWVKDTTLMDQVKEALEG
                     GITFLQLREKHLSKEEFIKEAREMKELSKEYKVPFVINDNIEVALAVDADGVHIGQND
                     MSV"
     assembly_gap    5293..5316
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5317..5634
                     /locus_tag="PLC71_04760"
     CDS             <5317..5634
                     /locus_tag="PLC71_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRM01986.1"
                     /translation="GVSAHNVEEAIKAQKGGADYLGVGAVCATSTKKDANVVSKEEIK
                     KICHTVEIPVVAIGGIKKENIKTLEGTDVDGVAVVSAIFAAKDIKKDTKQLRSLVEEM
                     KQK"
     gene            5750..6672
                     /locus_tag="PLC71_04765"
                     /pseudo
     CDS             5750..6672
                     /locus_tag="PLC71_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391496.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
     gene            6790..>7206
                     /locus_tag="PLC71_04770"
     CDS             6790..>7206
                     /locus_tag="PLC71_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HRM01987.1"
                     /translation="MIQLFSEGAYLVDGTTLVKESEAEALKQLTGEVVSKEEASKNTI
                     AYGILRDHNTSGNMEKLQIKFDKLTSHDITFVGIIQTARASGIEKFPVPYVLTNCHNS
                     LCAVGGTINEDDHMFGLTAAKKYGGVYVPPHQAVIHQ"
BASE COUNT         2464 a         1106 c         1677 g         1925 t
ORIGIN      
        1 aaacgtgaga caaacacaat gccacagtgg gcaggatcat catggtattt cttaagatac
       61 atcgataaca agaataatga agaattagta tctagagaga aagcagacaa atacttacca
      121 gtagatatgt atatcggtgg agtagaacat gctgtattgc atctgttata ttcaagattc
      181 tacacaaaat tcttatgtga catcggagtg atcgactttg atgaaccatt ccacaaactg
      241 ttcaaccagg gaatgatcac aggtaagaac ggtatcaaga tgagtaagtc caaaggaaat
      301 gtcgtatccc cagatgatct tgtaagagat tatggatgtg actccttaag aatgtacgaa
      361 ctgttcgttg gaccaccaga attagatgct gaatgggatg aaagcggtat cgatggagtg
      421 catagattct taacaagatt ctacaaactt atcatggatc agaaagacaa aggtgtagaa
      481 gctgataaag aattattaaa agtacgtcat aaactgatct acgatatcac aacacgttta
      541 aataacttca gcttgaatac agttgtgagt ggatttatgg aatataccaa tacattaaca
      601 gcaatggcta agaaatctgg agttgacaaa gaaactttag agacagcaat tgtattactc
      661 gcaccatttg caccacatat tgcagaagaa ttatgggaag caatgggaca tgaggacagc
      721 gtatttgctc agacatggcc aacatatgat gaagaagcta tgaaagagga tgaagtagag
      781 atcgttgttc agatcaatgg taaagtaaaa ggaaaacttg tcatcggtgc agaagaagac
      841 aaagattctg ttcttgcgaa agctaaggaa gttcttggag ataaattaaa cggtaatatc
      901 gtgaaagaaa tttatgtacc aggaagaatt gtaaatatcg ttcagaaata gaataataaa
      961 atggcgcaga taacgataac agtgtctgcg tcgttttttt ctcaagatca gtaaggatat
     1021 atgaagatca gaaaggctaa aacatgtata catttgacag cagaatccga tacagtgaaa
     1081 tagacagaag tggcagacta tcaattccgg cagtagttga ttatttccag gactgcagtg
     1141 catttcagtc tgaagaactg ggtgnnnnnn nnnnagtaga atatcttgcc aataagaaac
     1201 gtacatggat cctgaattca tggcaaatcg ttttggaaag aagaccagag gaatgtgaaa
     1261 agatcacagt tggaacatgg ggatctggat ttgacaagtt tcatgcaacg agaaatttta
     1321 tcatgaagac aacacagggg gaacgattag cttatgccaa ttctatctgg gtatatataa
     1381 acacagaaac aggaatgcca gtcagaccaa ccaaagaaga aattgatgtc tacaaattag
     1441 aagccccatt agagatggaa tatgaaccaa gaaagattaa actttccaga gaatgggaag
     1501 aaaaagaacc agtgaaagta caaagaagct ggatcgacag caatgatcat gtcaataata
     1561 gctggtatgt caaaacagca tttgaacagc tgccacaaga tttggagatc agacagctga
     1621 gggtcgaata taaaaaacag gcgtatgaag gagatattct atatccaaga tatgcaaggg
     1681 aagaacaaag gacactggta gcattgtgtg atgaaaaaca aaaaccatat gcagtgattg
     1741 aatgtagata gatattagat ggaaggaaaa gcagatgaaa cagacgaaga tcgtaatttt
     1801 aggagcggga catgtcggaa cacactgtgc attgtcattg atgtttcgtg gtcttgcaaa
     1861 tgaaattgtt ttaatcgaca cagatgaaga aaaagcaaaa agccaggcat tagatcttga
     1921 tgatatggga gcgtgtcttc ctgcgaaagt tgtgattcgt tcaggcagtt atgaagatct
     1981 tgacgatgcg gatattcttg tcaatgcgat cggacgaagc cgcaaggagg gagaaacaag
     2041 acttgacatg ttcggagatt ccatggaacg attgaaagat atcattccaa agattcaaga
     2101 tacaaagttt cgaggaattc tgatcagtat tacaaatcct gcggatgtag taggagaatg
     2161 tttgaggaaa gcgttaggta tcgagagatt ccgttgcttt agcacaggaa cgagtcttga
     2221 cagcttaagg atcaagagaa ttttagaaga gaaaacagga tatcacagaa attccataga
     2281 ggcattctgc atgggtgaac atggggattc tcagattgtt ccattatccc gtgtgtctgt
     2341 cggagggaaa ccatttgcaa aacttcaaaa ggagtatcct gatacgttag gcaagattac
     2401 gatcgaagat ctacaggacg aggttcgaca ggcgggcatg acagtgatca tcggtaagaa
     2461 aagtacagaa tttggaatag ggatcgcgtt aagtgggatc gtcaaatcaa tcgtatacga
     2521 tgagaaaagg atctggccgc tttctgtcca tctggatggg gagtatggac agaaagacgt
     2581 ggcagcagga gttccggccg tgatcggagc agacggaata gaagaaattg tagaaatgga
     2641 tcttacagaa gaggagaaaa gccaatttac gcattcctgt gatgtgatcc gcggttattt
     2701 aaagaaagca aaagaaatgc tttcttaaac ctaaattaca tgatcagtgg acggatcata
     2761 tcgatccgct gctgttcttc tggagacatg gattctttca gaccatcgat gatcatggaa
     2821 atttcttcgt ttgtaaaaga aagaccttgt tctgaaagac ggcttgaagc cgccataagg
     2881 tctgggagaa ttaattcaag aggttttccg tggcagctgg agatcacaaa ttttatgatt
     2941 tccagctttt ttgggtcaag agaagataaa tgatcgaatg aaataggatc gtcagacata
     3001 aaaaactcct ttattttttt agaatctggt ttaactggtt cataaagtca gaatttggtt
     3061 ttgctgtatt agtacaagga ctgtcttttg ccatcatcat aggcattagt tttgaaaata
     3121 gatcattgtt ttggctgtca gaagatgagg gagatgaatg gttagtcgta tcgacaaaac
     3181 gctgataatt ttgatagact tcaaacattt gcatcatctg aagaatttgt tcgatctgca
     3241 tagcggtttc cttcggggcg cgaagtttca gagcatttag taataattcc atagaagcag
     3301 gatcttgatc aaaaccacag gcagaaagag tctgttcgtt gtcaaattca gaagagattt
     3361 tggaaagttc ggttaatttg atataaatgg cagcaacctt ttttaagttt ggtgggagat
     3421 atggaagggc atcttctaac atattagaag agtcattacg gaaaaaattt tcccaggaag
     3481 tttgattttg ttccatgatt gtcacaacct tcagattttt tctcatatcc tatagtatta
     3541 ggaggtgaaa aaaattatga atgaaatttt ggtagaagaa gaaaatacag tttatgaact
     3601 ggatcctgag tgcttaaggc acaaagaaga acaaagacaa ctggaagaaa agaggaaaca
     3661 ggaaaaacaa gagaatgata actatcggat gcagaagaga ccagaaaaaa ggaatcagaa
     3721 caatatgtgg atcatctgtt ggctgttact ggtgatctgc caataaaaaa gaggatgccg
     3781 gatcaaaagg catcctcttt tatcagaaat cattaaattc agcatccaca tccgcagcca
     3841 ttgttgttgc cacatccaaa gctgccgtta ccacagcagc agaggattaa gatgatccag
     3901 atcaggctgt cacatccgtt tccgcatccg cagccatcat tatttccgcc tccacagcag
     3961 caaagtaaga ttaaaagaag cagacagctg ttatttcctc caaagcaaga tgaaccacaa
     4021 gatcctccac agccagttgc agctaaatca ctcatgttaa aacctccaaa agatttattt
     4081 acagtttata gtattctggg aattataaag tgtgagaaaa ttcgccagta tacctttttt
     4141 aatatttgtg gtataatctg caatgaatgc aggagacagg agaaaagaag gaatcagatg
     4201 aagagaatag attttgagag aatttttgat aatatcagaa gaaatcagac aatggtccat
     4261 tgtattacaa actatgtaac gatcaatgat gttgcgaata tgatccttgc gatcggggct
     4321 tcaccgatca tggcagatga ctggatggaa gttagagaga tcacatcaat gtgtgacagt
     4381 ctcgtgatca atatgggaac tttaaaacaa aatacagtga gatccatgct tcttgcggga
     4441 aaagaagcga atcaacgggg tcatctggtc gtgtttgatc ctgtgggagt cggtgcttcc
     4501 agattcagaa aagaaacagc ggcgaaattg cttaagcaga tccactttaa cgtgatccgt
     4561 ggaaatatat cagagatcaa aaccttatat gagggcagtg atgatggtta tggtgttgat
     4621 gcgaagaaag atgatgctgt gacagaagac aatttggaat atgtgattca aatggcaaag
     4681 aatatggcga agaagaccaa agcagtgatc gtgatcacag gaaaaacaga tctagtgacg
     4741 gatggacagc agatctattt gatcgataac ggtgttttgg atatgtcaag gatcacggga
     4801 accggatgta tgttggatgg tgtgatcgca ggatttatcg gagctaatcc agatcagatc
     4861 ttagaagcag tgacgacagc agttagtgcg atgggaatct gtggagaata tgcaaaggag
     4921 aaagcagaag gaacaggtac attgaaagtg catctgatgg atgcgatgag taatatgaat
     4981 gcagaatgga tggaaaggag cagtcaaatt gagagtaaat gctgatgcaa tgactttata
     5041 tgcagtaaca gacaggacat gggtaaaaga cactaccttg atggatcagg tgaaggaagc
     5101 tttggagggt ggaattacct ttttacagct tcgtgaaaaa catctttcca aagaagaatt
     5161 tataaaagaa gcaagagaaa tgaaagaact gtccaaggaa tacaaggtgc catttgtgat
     5221 caatgataat atagaagtgg ctttggcagt tgatgctgat ggagttcata tcgggcaaaa
     5281 tgacatgtca gtnnnnnnnn nnnnnnnnnn nnnnnngggg tttctgcgca caatgtagag
     5341 gaagcaataa aagcacaaaa aggcggtgca gactatttgg gagttggtgc agtttgtgca
     5401 acatcaacca agaaagatgc caatgttgtc tctaaggaag aaattaaaaa aatctgtcat
     5461 acagtagaga ttccagtggt agcgatcggc gggatcaaaa aagaaaatat caagacacta
     5521 gaaggaacgg atgttgatgg agtggcagtt gtttcagcaa tctttgctgc aaaggacatt
     5581 aaaaaagata caaaacagct gagaagcctc gtagaggaga tgaagcagaa atagaacaaa
     5641 acacgcggag tttatgaaaa taagaattgc aatattttaa atccgtgata tactagataa
     5701 ttgtgtgata aaaaagagtg tactaaaaag ataattagga ggaataccaa tgttaaatat
     5761 gattgccgga agtgttttac caatcacggc atttatcgga gtattttttg catttgctgc
     5821 aacctgtatc ctgacatcct ttttaaatga ttggctgcca agagatatgg gaagagaatt
     5881 tgcacataat ggaaagttat ccgcaggaaa accaagaggt gcagggatca tttttatttc
     5941 tgtattttgt atcgcagcat tgttgtttgg tttatttatc ttgttcttgt ttttgttgaa
     6001 atgttaacag gatttatgga tgatgcagca gatgagccat ggggagaata caaaaaagga
     6061 attcttgact ttctggtagc agcagttgca gcacttaact atattcattt taattctact
     6121 tatgtagagc ttgcaacgat cggcgtgaaa ttctatattc caccagtgtt gttctttatt
     6181 cttacgatca ttcttgtatg ggcagcgatc aatgtaacaa actgctctga tggagtcgat
     6241 ggactatctg gaacattaac gatcattaca ctggtaacga tctatgtgat cgatcagatc
     6301 cgtggaaatg aaacacagtt tccatatatt attttattgt ttgttgtatg tattcttgga
     6361 tatttatggt acaatgcaac accaagccgt ttgatgatgg gagatgcagg aagccgtgcc
     6421 atgggaattt ttatcagttt ggcagtgctt aagacaggaa gtcctttctt gttcttatta
     6481 gttgcattga ttctgatcct ggatggtgga ttaggacttg tgaaagtagc attacttaga
     6541 ttcttaaaga tacatatttt tgcgaataca acgatgccgt tacatgatca cgttcgtaag
     6601 aaaatcggtt ggtctaatac acagactgtt ttcagatttg cgatcattca gatcgttatt
     6661 tcaattaaat aaaaataaaa tatatagggt gcagagcctt ggtgtctgca cctttttaac
     6721 gttttacttc cataaagtgt agaagtatgt tataataact agaaaaatgc attgcaggag
     6781 gacagataca tgattcaatt attttcagaa ggtgcatatc tggtagacgg aacaactttg
     6841 gtaaaagaat ctgaagccga agcattaaag cagcttacag gtgaagtggt atccaaggaa
     6901 gaagcttcta agaacaccat agcatatggt attttaagag accacaatac atctggtaac
     6961 atggagaaac ttcagattaa attcgataaa ttaacatctc atgatattac gtttgttgga
     7021 attatccaga cagcaagagc atcaggaatc gagaaattcc cggtaccata tgtattgaca
     7081 aactgtcata atagtctgtg tgctgttggg ggaaccatca acgaagatga tcatatgttt
     7141 ggtttaacag ctgcaaagaa atatggtgga gtttatgtac cacctcatca ggcagttatc
     7201 catcag
//
LOCUS       DAOMUE010000074         7193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2747_length_7193_cov_2.376436, whole genome shotgun sequence.
ACCESSION   DAOMUE010000074 DAOMUE010000000
VERSION     DAOMUE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7193
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2747_length_7193_cov_2.376436"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PLC71_04775"
     CDS             complement(<1..828)
                     /locus_tag="PLC71_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HRM01988.1"
                     /translation="MDYAKIASQVIEHVGGKQNIKSVQHCATRLRLQLKDNDLRNEDA
                     VSDIEGVKGVFLTQSQFQIIFGSGLVNLVCDEVQKQLGISVDTPADAEKEEKKGNVLQ
                     RLVKLLSDIFVPIIPAIVAGGLLMGVNNILTAAMFSGKSVIDLYPQFKGLATAINMFA
                     SAPFAFLPVLIGFSATKKFGGNPYLGAAMGMIMVHPDLLSAYSIGIAKAPVWDIFGFK
                     IQAIGYQGTVLPVLAVAFILATIEKKLHKVTPTWLDNLTTPLISIMVTSFLTFIFVGP
                     "
     gene            complement(1065..2048)
                     /locus_tag="PLC71_04780"
     CDS             complement(1065..2048)
                     /locus_tag="PLC71_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM01989.1"
                     /translation="MAKKMTMADIANLSGVGKSTVSRYFNGGYVKDETRAKIQKVIEE
                     YNYTPNAFARLNAKQSNVIGVVVPTLNSKITSRVITSIDRYLREKGYTTLIQNSDHDI
                     DQELQNIQRLIQLNVDGILISSIAITKDHKELLKKAGIPVVVLTQDYEDGISIIYDDY
                     HAGKTIGEYIGKKGHQKVGYIGVYETDQAVGIERRNGVLDGLKEYGITPVTGKSDYSF
                     IGGQMVTRELLERESDLDAIICATDRLAFGAYKILHEHKKRIPEEVSVAAFGGYDEST
                     LLTPELTTLKFDSYGMGYLGAETILKMITGEPVSKKQIVGYEFIEGGSVRS"
     gene            complement(2102..3424)
                     /locus_tag="PLC71_04785"
     CDS             complement(2102..3424)
                     /locus_tag="PLC71_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM01990.1"
                     /translation="MKIEEINIDGFGKFHKYHCQTSGKLEVFYGKNESGKTTLRKFMI
                     AMLFGLEKSRGLAARYDDFTRYQPVNGGIYGGSMVFEKDGIRYKIMRNFGQGQTEYRI
                     FDADTMEELKGKEYLFESDQQAFENTVSMTQAEIRTGREMKEVLQNSMANLRSSKDAA
                     IDLRKAIDHLKARRRQMRKDPVFAQIDNLRRQQGSWQYDAQALNEYEQEEREIRKHLR
                     QKRKLTLLQKILLWFRKLFGGEDEERIRKIELRHRLEIIEIEKAQLMQQKEEADKKKQ
                     EYEILLGQKRKKELEIHEIELAMKAIEEAASQVQKTFGQELNEKISEIFCDMTNGKYT
                     QAVMDENFSMMVYDGFDHVDMKYLSNATVEQLYFALRLASADLLYENDAFPLFLDDVF
                     GNYDDERLAQTMQYLSHHTDRQIFLFTGRKEILRLLDEKEILYHLISL"
     gene            complement(3428..4468)
                     /locus_tag="PLC71_04790"
     CDS             complement(3428..4468)
                     /locus_tag="PLC71_04790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HRM01991.1"
                     /translation="MKFIHIADVHWGARPEREQTFGRIREQEIKETFQRVIDHANKQQ
                     VDLLLIAGDLFDQPPTQQELREVDYLLSRLNHTRTVLIAGNHDHLEPHDVFSQYKWNS
                     EVYLLDGKQRDHISFEDLETTIYGFSYWKNQITKPLYDRMKPDESESSDFSILLAHGG
                     DESHIPIQREALKWSGFDYIALGHIHKPEIIFEDLMAYAGSLEPLDHTETGYHGLIEG
                     EISEEKKQITFVPFAKRMYTQVEVMISDEMSALEIVDRVETELSYRGRENIYEIVLTG
                     SIDPQIHLDFYEITSQYMVTDIIYETRDQWEYEQLSEEDDFIIQKVADILKDEPEALR
                     YAMEALTYAREK"
     gene            complement(4503..4850)
                     /locus_tag="PLC71_04795"
     CDS             complement(4503..4850)
                     /locus_tag="PLC71_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01992.1"
                     /translation="MKYSKTIAGITIEICEKAIGEDLVLTLSGGEKPHIGCVVQAVPR
                     PSLSGDGTISVTSSVINLTGHKDEFLCRRLAENRCRETGKVVVCTGGVHMDHITGEQI
                     EELLKNIDRMMTE"
     gene            complement(4957..5874)
                     /locus_tag="PLC71_04800"
     CDS             complement(4957..5874)
                     /locus_tag="PLC71_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRM01993.1"
                     /translation="MDTTLIIMAAGIGSRYGAGIKQLAKMGPNGEIIMDYSIRDAKEA
                     GFNKVVFIIRKDIFEEFEEIIGSRIKDQIDVEYVFQELDDLPEGFEVPEGRTKPWGTG
                     QAVLCCKDVVKEPFVIINADDYYGKEAFVKLHDFLVSGEDLGREFTMGMAGFILKNTL
                     SDNGTVTRGVSVVDENGLLSQVHETTGIMMGEDGKIKCDLPDVQEWISPEDKVSMNMW
                     AAYPEFLEFLAEDFKTFLSEVEEGDLKKEYLLPNIVDKLLKEGRANVKVLETQDRWFG
                     VTYQEDKEAVQKAFAGLIKEGVYPANLWG"
     gene            complement(6102..6245)
                     /locus_tag="PLC71_04805"
     CDS             complement(6102..6245)
                     /locus_tag="PLC71_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM01994.1"
                     /translation="MHLLGTIAMILVLSLMIAGLLKLNYKDLWVSEDKTSREPEARRL
                     RSC"
     gene            complement(6482..7114)
                     /locus_tag="PLC71_04810"
     CDS             complement(6482..7114)
                     /locus_tag="PLC71_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM01995.1"
                     /translation="MKTIIGKITIDILTSIYQPFWFAILLSIMTMFFYLYVKCPEGAG
                     KGWKAAIRTWIDHFKRDREFRKKYILFFYITMVLFKTLINRDMWMNPLCDVWGVWGIY
                     RYNTVTGQRILTSECIENFFLLMPYIVLMFWNFEEKIFGKKVYIGKIILKSIQIAFLS
                     SLTIELLQLFLRLGTFQISDLFFNTIGGLAGGIIYFLVNAVAERIRRMRN"
BASE COUNT         1905 a         1711 c         1072 g         2505 t
ORIGIN      
        1 tgggcctaca aaaataaatg ttaaaaatga tgttaccatg atagagatca atggtgttgt
       61 caggttatct aaccatgttg gtgttacttt atgtaacttc ttctcgatcg ttgctaaaat
      121 aaatgcaact gctaatactg gaagtacagt tccctgatat ccgatcgcct ggatcttaaa
      181 tccaaagata tcccatactg gtgctttggc gataccgatg ctgtatgcac ttaacagatc
      241 tggatgaacc atgatcatac ccatggctgc tccaagatat ggatttccgc caaacttctt
      301 cgttgcgcta aatccgatca atactggcaa aaatgcaaat ggagcacttg caaacatgtt
      361 gatcgctgtt gccagaccct taaactgtgg gtacagatca atgacggact tgccactaaa
      421 catcgctgct gtcaggatat tgttcactcc cattaacaga ccacctgcta cgatggctgg
      481 aatgatcggt acgaaaatat cacttaataa ctttactaat ctctgaagaa catttccttt
      541 cttctcttct ttctcagcat ctgctggtgt atcgactgag attcctaact gcttctgtac
      601 ttcgtcgcac actaagttta caagtcctga tccgaaaatg atctggaact gtgactgtgt
      661 taagaacact cccttaactc cttcaatgtc gctgactgca tcttcattgc gaagatcgtt
      721 gtcttttaac tgtaaacgaa gtcttgttgc acaatgctga actgacttaa tgttttgttt
      781 gccaccaaca tgctcgatga cctggctggc gatttttgcg taatccatat cttctctcct
      841 tttttattgt gaaacttatt atggtatcgg ttccatatct gtatcttata actttctctt
      901 ctatttgtca atagtattta tggaatcgat accacatttt tttattttat tacttttatt
      961 tgtgttttat gtgagacaac ttaacaaagc aacaggtaaa cggcaaagcc ctccgttccg
     1021 aataatacgg aaggagggcg atattataag cacgtatgaa gaatttatga tctgacactt
     1081 ccgccttcaa taaattcata tcccacaatc tgtttcttgg atactggttc tcctgtgatc
     1141 atcttaagga tcgtttccgc tccaaggtat cccattccat aggaatcaaa ctttagcgtt
     1201 gtaagctctg gcgtcaggag ggtactttca tcatatcctc caaacgctgc cacagacact
     1261 tcttctggaa tcctcttctt atgttcgtgt aaaatcttat atgcaccaaa cgcaagccga
     1321 tctgttgcac agatgatcgc atctaaatct gattccctct caagtaattc tcttgtaacc
     1381 atctgtccac caataaaact ataatcactc ttccctgtca ccggtgtgat cccatattct
     1441 ttcaatccat ctaacacgcc atttctacgc tcgatcccaa ctgcctggtc tgtctcatag
     1501 acaccaatat atccaacctt ttgatgtcct ttcttcccga tatactctcc gatcgttttc
     1561 cctgcatgat aatcatcata aataatggaa attccatctt cataatcctg tgttaaaaca
     1621 accactggaa ttcccgcttt ctttaacagc tctttgtggt cctttgtgat cgctatggaa
     1681 ctgatcaaga tcccatccac atttaactgg atcaggcgct ggatattctg taattcctga
     1741 tcaatatcgt ggtctgagtt ctgaataagg gttgtatacc ccttttctct taaataccga
     1801 tcgatacttg tgatcacacg gcttgtgatc ttagaattta atgtcggcac aactacaccg
     1861 atcacgttgc tttgtttcgc attcaatctt gcaaatgcat tcggtgtata gttgtattct
     1921 tcaatgacct tctgaatctt cgctctcgtc tcatccttta catatccccc gttaaaatag
     1981 cgggatactg tgctttttcc aactcctgat aaattcgcga tgtctgccat ggtcatcttc
     2041 tttgccatat attttgatct cctttgttac tttatctctc attatcttag cacagaaaaa
     2101 gctataaaga aataagatga tataaaattt ctttctcatc caataaacga agtatttctt
     2161 tcctgcccgt aaataaaaag atctgacggt ctgtatggtg acttaaatac tgcatcgtct
     2221 gtgccagtct ttcgtcatca taattaccga acacatcgtc taaaaacaac ggaaatgcat
     2281 cattttcata taaaagatcg gccgatgcaa ggcgcagtgc aaagtataat tgttctaccg
     2341 tggcattgct taaatacttc atatcgacat gatcaaatcc atcataaacc atcatcgaaa
     2401 aattctcatc catgactgcc tgcgtatatt ttccattcgt catatcacaa aaaatctctg
     2461 agatcttctc atttagttcc tgtccaaaag tcttttgtac ctggcttgca gcttcctcga
     2521 tcgctttcat tgcaagttct atctcgtgaa tctcaagttc tttcttcctt ttctgcccca
     2581 gcaaaatctc gtattcctgc tttttcttat ctgcctcttc cttttgctgc ataagctgtg
     2641 ctttctcgat ctctatgatc tctaagcgat gtcttagttc tattttgcga attcgttctt
     2701 catcttcccc accaaataac tttcgaaacc acagcaaaat cttctgaagc agcgtcaact
     2761 ttctcttttg tcttaaatgt ttccgaatct ctctctcttc ctgttcatat tcatttaacg
     2821 cttgtgcatc atactgccat gacccctgct gtcttcgcaa attgtcaatc tgtgcaaaca
     2881 caggatcttt tcgcatttgt cttcgtctgg ctttcaaatg atcgatcgct tttcgaaggt
     2941 cgatcgcggc atccttgctg cttcgcagat tcgccatgga attttgcaaa acttccttca
     3001 tctctctacc tgtacgaatc tctgcctgtg tcatcgaaac cgtattctca aatgcctgtt
     3061 gatcggattc gaataagtat tccttcccct taagttcctc catcgtatct gcatcaaata
     3121 tgcgatactc tgtctgccct tgaccgaagt ttcgcatgat cttatatctg atcccgtcct
     3181 tttcaaagac catggagcca ccgtaaatgc cgccatttac tggctgatat ctggtgaaat
     3241 catcatatct tgcagcaagt cctcttgatt tctccagccc aaacagcatt gcgatcataa
     3301 acttacgaag tgttgtcttg ccagattcat tcttgccata gaaaacttct aacttcccag
     3361 atgtctggca atgatattta tgaaattttc caaatccatc aatattgatc tcctcaatct
     3421 tcatctttta cttctccctg gcatatgtta aagcttccat cgcataacgc aaagcctctg
     3481 gttcatcttt taaaatatct gcgaccttct ggatgataaa atcatcttcc tctgataact
     3541 gttcgtattc ccattgatcc ctcgtttcat aaatgatatc tgtaaccata tattggctcg
     3601 tgatctcata aaaatccaga tggatctgtg gatcaatact tcctgtcagt acaatctcat
     3661 atatattctc tcttccacga tatgaaagtt ccgtttctac ccgatctacg atctctaacg
     3721 ctgacatctc atcggatatc atgacttcca cttgtgtata cattctcttt gcaaagggaa
     3781 cgaaggttat ctgtttcttt tcttcgctga tctccccttc gatcagccca tgatatcccg
     3841 tctctgtgtg atccaatggt tccagcgatc ctgcatatgc catcagatct tcgaaaatga
     3901 tctctggttt atggatatgt ccaagagcga tatagtcgaa tccagaccat tttaacgctt
     3961 ctctctgaat cgggatatga ctttcatccc ctccatgtgc cagcaggatt gaaaaatcac
     4021 tgctttcgct ctcatctggt ttcatgcgat catacaatgg tttcgtgatc tgattcttcc
     4081 aataactaaa gccataaatg gtcgtttcaa gatcttcaaa agaaatatga tctctttgtt
     4141 ttccatccag aagataaact tccgaattcc atttatactg ggaaaataca tcatgcggtt
     4201 ccagatgatc gtgattccct gcgatcaaaa ccgttcttgt atgattcaat ctggacaaca
     4261 gataatccac ttctctcagc tcctgctgcg ttggcggctg atcaaacaga tcccctgcga
     4321 tcaataacag atctacctgt tgcttattcg catgatcaat gactctttga aatgtctctt
     4381 tgatctcttg ttctctgatc cttccaaatg tctgctctct ctctggtctg gctccccaat
     4441 gaacatccgc aatatgaata aatttcaaaa aatcacctgc tttctgatca tcttctatga
     4501 tcttattctg tcatcatacg atcgatattc tttaataatt cttcgatctg ttctcccgtt
     4561 atatgatcca tgtgcacccc gcctgtacat accactactt ttcctgtttc cctgcatcga
     4621 ttctcagcca gtctgcggca tagaaattcg tctttgtgac ctgtcagatt gatcacagat
     4681 gaagtgacac tgatcgttcc gtcaccactc aacgaagggc gtggaactgc ctgaacaaca
     4741 cagccaatat gcggtttctc accaccgctt agagttaaaa caagatcttc tcctattgct
     4801 ttttcacaga tctctatcgt aattcccgca attgttttgc tatatttcat tttgatcctc
     4861 cttattctat tccaaagcgg acctcaccaa aacttcctct gaaataaaac aggcagccag
     4921 ttcaacactg actgccccta aattatgcca tatatcttag ccccataaat tcgctgggta
     4981 gacaccttct ttgatcaatc cggcaaatgc tttctgtaca gcttccttat cttcctgata
     5041 agttacaccg aaccatcgat cctgtgtctc taatactttg acgtttgcac gtccttcttt
     5101 caataatttg tctacgatgt ttggtaataa atattctttc ttcagatctc cttcttctac
     5161 ttcacttaag aaagtcttga agtcttctgc aaggaactct aaaaattctg gatatgcagc
     5221 ccacatattc atagaaacct tatcctctgg actgatccat tcctgaacat ctggaagatc
     5281 acacttgatc ttaccatctt cccccatcat aattcctgtt gtctcatgaa cctggcttaa
     5341 taatccattc tcatctacaa cactaactcc tcgtgtgaca gttccattgt cacttaatgt
     5401 attcttcagg ataaatccag ccattcccat tgtgaattct cttccaagat cttctccaga
     5461 tactaagaaa tcatgaagct tcacaaaagc ttcctttcca tagtaatcat ctgcgttgat
     5521 gatcacaaat ggttccttga caacatcctt gcagcaaaga acagcctgtc ctgttcccca
     5581 aggctttgta cgtccttctg gtacttcaaa tccttctgga aggtcatcta attcctgaaa
     5641 tacatattct acatcaatct gatctttaat tcggctgccg attatctctt caaattcttc
     5701 aaagatgtcc ttacggataa taaatacaac cttattaaat ccagcttctt tcgcatcacg
     5761 aattgaataa tccataatga tctcaccatt cggtcccatc ttcgcaagct gtttgattcc
     5821 tgcaccgtat cttgatccga ttccggctgc cataattatt aatgtagtat ccataaatct
     5881 ccccttcagt catctgacct gttttgttac ctgtatactt gttatatcct gtgtaaagca
     5941 ttccatatat ttgtcttcac atataccttt tattataaat attattctct ataatgtcaa
     6001 tagattctgg ctatcatttt cttaaatact tccttattgt aagatcaaaa agagcaggta
     6061 ctctgtttcg tacctgctct tttatctcat tggtcttata cttaacaact tcttaatctt
     6121 cttgcctctg gctctctgga tgtcttatct tctgataccc ataaatcttt gtaatttaat
     6181 tttaaaagcc ctgcgatcat taagcttaac actaagatca ttgcaattgt tcctaataaa
     6241 tgcattgtta ttctcctctt tatattgtgt tgtattatgt atcctgtatt tatgctttat
     6301 attttatcaa attaatttcg gagaatcaat atgtagcatt attttctagg aaattacaac
     6361 atttcgtttg tattttccct ctggtgttag caaaattttc agtaataaat ctgtcttcgt
     6421 tattgttttt gtgcaatttg tagtaccaat ttctgaatgg agtgtttgtg ttttgtcgta
     6481 tttaatttct cattcgtcta atccgttctg ctacggcatt cactagaaaa taaataattc
     6541 ctcctgctaa tcctccaatt gtattaaaaa ataaatctga tatttgaaat gttcctaatc
     6601 ttaaaaacaa ctgcaacagc tcaattgtca gagatgacag aaacgctatt tgaatacttt
     6661 ttaatatgat tttccctata tacactttct taccaaatat tttttcttca aaattccaaa
     6721 acattaatac tatatacggc ataagcagga aaaaattttc tatacactct gatgttaaaa
     6781 tgcgctggcc tgtcactgta ttatatctgt atattcccca tactccccac acatcacata
     6841 atggattcat ccacatatct cggttgatca acgtcttaaa taataccatg gtaatataga
     6901 aaaacaatat atatttttta cgaaattctc tgtctctttt aaaatgatcg atccatgtcc
     6961 ggatagctgc tttccatccc ttgcctgctc cttctggaca ttttacatac aaataaaaga
     7021 acattgtcat tattgataac aaaattgcaa accaaaacgg ctgatagatt gatgttagaa
     7081 tgtctattgt gatttttcct atgatagtct tcattcttgt gccttctcct attctatgtg
     7141 ttaaatgcgt attttgtttg gaatattttt aatagatttc tgaatgtcct tgg
//
LOCUS       DAOMUE010000075         7084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2805_length_7084_cov_2.084080, whole genome shotgun sequence.
ACCESSION   DAOMUE010000075 DAOMUE010000000
VERSION     DAOMUE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_7084_cov_2.084080"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1618
                     /locus_tag="PLC71_04815"
     CDS             143..1618
                     /locus_tag="PLC71_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HRM01996.1"
                     /translation="MKKEKKSSIFWILIIFFVLFSIWQLWVPEALDDWRWGSEVGIMR
                     LKTWFEGYNGRYFGDILVIVLTRLPIVLRSMAISGIMTAMLYLIYQLTEYKILSFLIC
                     VFFVVNIPTSLMSQTYGWVSGFSNFCVSATMILGFLLYHISIFKEKKVSKLKTGFSMI
                     NAFLGSLVLENSTVYLIVLSIAFLIIYGVQYKKLHKGMLLHLIMVGIGAFWMFSNSAY
                     RMAAAHTSQGTGKEIAAFELSFEWFASALKVYADTIINYWIMPNHLLNIVTCGMILIL
                     LQKRKKKGQGLFLLLFSGFLAFYIFNFMNLGIWKDYLAWGNAAQAVIATTYCLALVAF
                     SAWFITDFKDKVIVQIILWSQLILMGPLIVASPIAIRCFYVTYIFFVLYLGVLLSYSI
                     RSGLLKISKGYLNTGIMMCIFFVMIGNIGVYYFVHGQVNDRLQYIQTQIKQGEKKIEL
                     KEIPLGDTYCYGLDVYNPAWVWRYKMYYNIPGDIELKFPER"
     gene            1628..5065
                     /locus_tag="PLC71_04820"
     CDS             1628..5065
                     /locus_tag="PLC71_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2
                     protein/CDP-glycerol:glycerophosphate
                     glycerophosphotransferase"
                     /protein_id="HRM01997.1"
                     /translation="MREIKVSVIIPIYNVEEYLEECLQSVVDQTLEDLQVIMVDHGSL
                     DGSTDIAKKFASRYDHFEYVRQVNGGLGNARNTGVKYAKGKYIIFLDSDDIVPDDAYE
                     KMYLAAEKNHSEMVVGSVARFNSKKDHVSNLHEIAFRKYIDKTHITDNTDLIYDTTSW
                     NKLILKEFWDRNHFEFPERILYEDIPVTIPMHYLANNVTMVQDVCYRWRIRDGANKSI
                     TQRADDFTNMRDRITVLRMVDKFFEENVKEQELWDAKYYKWLYIDLMIYVNNCIYLSD
                     NRTLEMMKIIKDYIEETIPLETIDKLPVLYREKYVALMNLDEKRLVKLRQYEVDNYKN
                     LKIVKKGNKYIGKFPKAIVTGDKADMTEALDQWRLTQLIYDVAWQKEQCVIEGYVFLR
                     GLSVPNVNVQKLSAHLVCLSTGEKIPLEIQSIKSQYAQKKFGLKIDNETKQIHLANYK
                     GCGYRIILDAAKIRELKLDGEYHILLTYERDRWKKETILRGILKSLGNKLDKKTYFKD
                     HMLIELSKSYRYDFKVKISQNNIELNDMKLDGDQLRLKLSEKVDALYEAKDAHNAEIL
                     KAAITQEDVSVDISDIPENKRYIAVKKGNLFIPVYKEKKKRIFVENQKNQLVEETSGD
                     HRCYLLNRKAVPVIRDVKQNEEQFSFEIINKNIGNWQRATLYVEDPLEEEKIILGTGS
                     VNQHGEEEKVVISLSLKDEKIIKNLYARRRQVFILYENNEQQKVCALGGEHKVFDKKY
                     YTKERRYRFIIDPEDDFLYFTTLRVKEFLTRSAKKRAFVNRFLYPLLRLLPLKKKWIV
                     FESMWGSKFSCNPRYLYEYIDKNHPDYTCIWSLKDECIPITGNGVRVRRLSWKYLYYM
                     ARAKYFVNNVNFADSYEKRKGQIEVQTMHGTPLKTIGLDVPGDFPTKKSEKKYIRKCK
                     RWDYLIVQSKFVADLAPSAFKFENTIMDTGYPRTDILYSSNNEEEMGRLKEKLGLPKD
                     KKVIMYAPTWRVRDKFELMLDLEKMKEKFSDEYVLILRLHHFSAKGWKGVPADGFVYD
                     LTDYESIEDLYIITDILITDYSSVMFDYAVLDRPMLFFTYDLDEYRDKLRGFNIDIEK
                     EAPGPLLYTSDEVLDAIEHLDETIENSKQRVDAFHETYIPYECENSSEKVFNMMQGK"
     gene            5192..>6145
                     /locus_tag="PLC71_04825"
     CDS             5192..>6145
                     /locus_tag="PLC71_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HRM01998.1"
                     /translation="MKFIYAWYYKHGKINEKQALFESFHGKDVSDSSLAILQEFLKMP
                     ESKDFKIYFATNDKKRDQKFIDSIGLKVELVDIADFKYVKVLATSKYLINNSSFPAYF
                     IRRDEQVYLQTWHGTPLKTLGKRMRFGIESMYNVQHNFLHANYIMFPNEFTRKVIMED
                     YNLEALYTGTVVMNGYPRNSIFMDHEKADQVTKKLGNEDYTTMAYMPTWRGQSNHDVN
                     TSEYSREINQLLHYLDENLKDDQKLYVNFHPIVQKFVKLDNYEHIYPFPSGVDKYEFL
                     NSVDALITDYSSVFFDYSITRKPIILYMYDYEEYMHDRGMYF"
     assembly_gap    6146..6155
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6156..>7084
                     /locus_tag="PLC71_04830"
     CDS             <6156..>7084
                     /locus_tag="PLC71_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol--poly(glycerophosphate)
                     glycerophosphotransferase"
                     /protein_id="HRM01999.1"
                     /translation="PFRKIYDKEALRDCLVNETFREDSYENDMEYTEKYIQYDSIDAA
                     KNMAELVFHGNMCDMPTIDYSFNKEKKRRVLHYPNVKTAQTIDTIAQIAKKDEDVVIF
                     EKRFFNPELSSHLHDNYLDDFDYIFITRTLPRTYAEEVLKRKSAKIRKELHKREIKRC
                     FADLPVYSKFETEYYHGEAGETFSFNKKKMINASMQVMEDHLEIKFEEGKKGQPKKLL
                     LVNKKSQIIYTRDLTEKEWKDHCIKEYFDEFLSLEIADMNGRYQLMIELEDENGNKLP
                     YFFVDRKEYAKRKEVMDELDKSSVYMTGIKKHD"
BASE COUNT         2588 a          873 c         1468 g         2145 t
ORIGIN      
        1 atgcatggtt agattatata gtacttatgg caatttcatt ggtgacatcg attgtattag
       61 aatggtttaa gaaattaatt cgatatgaga agtttataga ttgggtcaaa agattagtaa
      121 caaaagaagg agcaacagta cattgaaaaa agaaaagaaa tcatcaatat tttggatatt
      181 gatcatattt tttgtattat tttccatttg gcagttatgg gtgccggaag cactggatga
      241 ttggcgatgg ggaagtgaag tagggatcat gagattaaaa acgtggtttg aaggatataa
      301 tggacgttac tttggagata ttttggtcat tgttttaaca agattgccga tcgtattgag
      361 aagtatggct atttctggga taatgactgc aatgttatac ttgatatatc aattaacaga
      421 atataagata ttatcatttt taatttgtgt tttttttgta gtcaatattc caacaagctt
      481 aatgagtcag acgtatggat gggtatcagg attttcaaat ttctgtgtta gtgcaacgat
      541 gattcttgga tttttgctat atcatatatc tattttcaaa gaaaagaaag tgtcgaagtt
      601 gaaaacaggg ttttcaatga tcaatgcatt tcttggaagc ttggttcttg aaaattcaac
      661 ggtatatcta attgtactaa gtatcgcttt tttaattatt tatggtgtac aatataagaa
      721 gctacataaa ggaatgttac ttcatttgat catggttggt attggtgcat tttggatgtt
      781 ctcgaatagc gcatatcgta tggctgcggc acatacatca cagggaacag gaaaagagat
      841 cgcagcgttt gaattaagct ttgaatggtt tgcctctgca ttaaaggtat acgcagacac
      901 gatcattaac tattggatca tgccaaatca tttattaaat atagtgacat gtggaatgat
      961 tttgatattg cttcaaaaga gaaagaagaa aggacaagga ttgttcttgc ttctgttcag
     1021 tggtttttta gcattttata tatttaattt tatgaatctt ggtatatgga aagattattt
     1081 ggcatgggga aatgcagctc aggctgtaat agcaacgacg tattgtttag cattggtggc
     1141 ttttagtgca tggtttatta cagattttaa ggataaggtg atcgttcaga tcattttatg
     1201 gtcacaactg attttaatgg gaccattgat cgttgcaagt ccgatagcaa ttcgttgttt
     1261 ttatgtaaca tatatctttt ttgttttata tcttggagta ttgctatcgt atagtatacg
     1321 aagtggactt ttaaagataa gcaaaggata tttaaacaca gggatcatga tgtgtatttt
     1381 ctttgtgatg atcggaaata ttggtgtata ttattttgtt catggacaag tgaatgacag
     1441 acttcaatat atacaaacac agataaaaca aggagagaag aagattgaat taaaggaaat
     1501 accacttgga gatacatact gttatggttt agatgtatat aatccagcat gggtatggcg
     1561 ttataagatg tattataata tcccaggaga tattgaattg aaattcccag agagatagga
     1621 gtaagtgatg agagagataa aagtaagtgt tatcatacca atttataatg tagaagaata
     1681 tttggaagaa tgtctgcaat cagttgtaga tcagacatta gaggatctgc aggtcatcat
     1741 ggttgatcat ggttctttgg atggaagtac agacattgcc aagaaatttg ctagtcgata
     1801 tgatcatttt gagtatgtaa gacaggtaaa tggtggactt ggaaatgcga gaaatacagg
     1861 agtgaagtat gcaaaaggaa aatacattat ttttcttgat tcagatgata ttgttccaga
     1921 tgatgcatac gagaaaatgt atcttgcagc agagaaaaat catagcgaga tggtagttgg
     1981 aagtgttgcg agatttaact caaagaaaga ccatgtgtcg aatctgcatg aaattgcatt
     2041 tagaaaatat atcgataaaa cacatattac agacaatacg gatctgattt atgatacaac
     2101 ctcatggaat aaattaattt tgaaagagtt ttgggatcgt aatcattttg aatttccaga
     2161 gagaattctc tatgaagata ttccagtaac gattcctatg cattatcttg ctaataatgt
     2221 gacaatggtg caggatgtct gttatcgttg gagaattcgt gatggagcta ataagtctat
     2281 tacgcagaga gcagatgact ttacgaatat gagagatcgt attaccgttt tacgtatggt
     2341 agataaattc tttgaggaga atgtcaaaga acaagaatta tgggatgcaa aatattataa
     2401 atggttatat attgatctga tgatctatgt gaacaactgt atttatcttt cagataacag
     2461 aacactcgag atgatgaaga tcattaaaga ttatatagaa gaaacaattc ctttagaaac
     2521 gatcgataag ttaccagttt tatatcgtga aaaatatgtg gctttgatga atctggatga
     2581 aaaacgatta gtaaaactgc gtcaatatga agttgataat tataagaatt taaagatcgt
     2641 gaaaaagggc aacaaatata ttggaaaatt cccgaaagcg atcgtaactg gtgataaggc
     2701 agatatgaca gaggcattag atcagtggag acttactcaa ttgatttatg atgtggcatg
     2761 gcagaaagag caatgtgtga tcgaaggata tgtattctta cgaggattat ctgtgccaaa
     2821 tgtaaatgta caaaaattat cagcacattt ggtatgctta agtacaggag agaagattcc
     2881 actggaaata caaagtatta agtcacaata tgcacagaag aaatttggct taaaaattga
     2941 taacgagaca aaacagatcc atttagcaaa ttataaaggt tgtggatacc ggatcatact
     3001 ggatgcagcc aagatcagag aattaaagct tgatggagaa taccatatat tattaactta
     3061 tgagagagac agatggaaga aagagactat tttaagagga atcttaaaat ctcttggcaa
     3121 taaattggac aagaaaacat attttaaaga tcatatgctg atcgaactta gcaagtctta
     3181 cagatatgat tttaaagtaa aaatttcaca aaacaatatc gaattaaatg atatgaaatt
     3241 ggacggtgat cagttaagat taaaactttc tgagaaagtt gatgcattat atgaagcgaa
     3301 agatgcacat aatgcggaaa tcttaaaagc agctataaca caggaagatg tttctgtgga
     3361 tatttcagat attccagaaa ataaacgata tattgcagtt aaaaaaggta atttatttat
     3421 tcctgtatac aaagaaaaga aaaagagaat ctttgtagaa aaccagaaga atcagttagt
     3481 agaagaaaca tcaggagatc atagatgtta cttgttaaat cgaaaggcag tacctgtgat
     3541 acgagatgta aaacagaatg aggaacagtt ttcatttgag atcataaaca aaaatatcgg
     3601 taactggcag agagcaacat tgtatgtaga agatccatta gaagaagaaa agattattct
     3661 tggaacagga tccgtgaatc aacatggaga agaggagaaa gtagtcattt ctttaagttt
     3721 aaaggacgaa aaaatcatca agaatcttta tgcaagacgt cgtcaggtgt ttattttata
     3781 tgaaaataat gaacagcaga aggtatgtgc acttggtggg gaacataaag tttttgataa
     3841 aaaatactac acgaaagaaa gaagatatcg atttattatt gatccagaag atgattttct
     3901 gtattttact acgctgaggg tcaaggaatt cctgacaaga agtgcgaaga aaagagcttt
     3961 tgtcaacaga ttcttatatc cgttattaag acttcttcca ttaaagaaaa aatggatcgt
     4021 atttgaatct atgtggggaa gtaaatttag ctgtaaccct agatatttat atgaatatat
     4081 tgataagaat catccagatt atacgtgtat ctggtcatta aaagatgaat gcattccgat
     4141 cacgggaaat ggtgttagag tcagaagact atcttggaag tatttatatt atatggcaag
     4201 agcaaaatac tttgtgaata atgtaaattt tgctgattct tatgagaaga gaaaagggca
     4261 gattgaagta cagacgatgc atggaacacc attaaagaca attggattgg atgtaccagg
     4321 cgattttcct acaaagaaat cagaaaagaa gtatattaga aagtgcaaac gttgggatta
     4381 tctgatcgtg caaagtaagt ttgttgcgga tcttgcacca tctgcattta aatttgaaaa
     4441 tacgatcatg gatactggct atccaagaac agatattttg tattcttcta ataacgaaga
     4501 agaaatgggc agattaaaag aaaaattagg acttccaaaa gataagaaag tcatcatgta
     4561 tgcaccaaca tggagagtgc gtgataagtt tgaattaatg ctagatcttg agaagatgaa
     4621 ggaaaaattt tcagatgaat atgtattgat cttaagactg catcactttt cagcaaaagg
     4681 ttggaaagga gttccagcag atggatttgt gtatgatctt acggattatg aatctattga
     4741 agatttatat atcattacag acatcctgat cacagattat tcttctgtaa tgtttgatta
     4801 tgcagttctc gacagaccaa tgttattctt tacatatgat ctggatgaat atagagataa
     4861 gcttcgtgga tttaatattg atatcgaaaa agaagcacca ggaccattac tatatacgtc
     4921 tgatgaggtg ttagatgcga ttgagcatct ggatgaaacg attgagaatt caaaacagag
     4981 agtcgatgca ttccatgaaa catatattcc atatgaatgt gaaaattcat ctgaaaaagt
     5041 atttaatatg atgcagggaa aataaaaaat aaacaatgcc ataaggcaga acaatcttta
     5101 agaaggtcat tgaaaggttt aagaaggaaa catggataaa ataagaaaaa ttattcgatt
     5161 tgtaaaaaga ttatttccaa aaactccaaa gatgaaattt atctatgcct ggtattataa
     5221 acatggcaag atcaatgaaa aacaagcact ctttgagtca tttcacggca aggatgtgtc
     5281 agattcatcc ttggcgatct tgcaggagtt tttgaaaatg ccggaatcaa aagatttcaa
     5341 gatctatttt gcgacgaatg ataagaaacg tgatcagaaa tttattgatt ctatcggatt
     5401 gaaagtagaa cttgtagata ttgcagattt taaatatgta aaagtgcttg caacatccaa
     5461 gtatctgatc aataacagtt cttttccggc gtatttcatt cgaagagatg aacaggtgta
     5521 cctacagaca tggcatggaa caccgttaaa gacattagga aaacgaatgc gttttggaat
     5581 tgaatccatg tataatgtgc aacataactt cttacatgcg aactacatca tgttcccaaa
     5641 tgaatttaca agaaaagtga tcatggaaga ttataatctg gaagctttgt atacaggaac
     5701 tgttgtgatg aatggatatc caagaaacag tatttttatg gatcatgaga aagctgatca
     5761 ggtaacaaag aaacttggaa atgaagatta tacaacgatg gcatatatgc cgacatggcg
     5821 tggacagagt aatcatgatg tgaatacgtc tgaatatagc agagagatca atcagctgtt
     5881 acattatcta gatgagaatt taaaggatga tcagaagtta tatgtaaact ttcatccaat
     5941 tgtgcagaag tttgtaaagc tggataatta tgaacatatt tatccatttc caagtggtgt
     6001 tgacaaatat gaatttttaa atagtgttga tgcactgatc acggattact caagtgtatt
     6061 ttttgattac tcgattacaa gaaaaccaat tattttgtat atgtatgact atgaagaata
     6121 tatgcatgat agaggaatgt atttcnnnnn nnnnntgcca tttagaaaga tctatgacaa
     6181 ggaagcatta agagattgtc tggtcaacga gacattccgc gaggattctt atgaaaatga
     6241 tatggaatat acagaaaaat atattcagta tgattctatt gatgctgcaa agaatatggc
     6301 agaacttgta tttcatggaa atatgtgcga tatgccaacg atcgattaca gttttaacaa
     6361 agaaaagaaa cgaagagttt tgcactatcc aaatgtaaaa acagcacaga cgatcgatac
     6421 gatcgcgcag atcgctaaga aagatgaaga tgtagtgatc tttgaaaaac gtttcttcaa
     6481 tccagaatta agcagtcatc tacatgataa ttatcttgat gattttgatt atatttttat
     6541 tacacgaaca cttccaagaa cctatgcaga agaagtgtta aaaagaaaga gtgctaagat
     6601 cagaaaagaa cttcataaga gggagatcaa gagatgcttt gcagatcttc cagtatattc
     6661 aaagtttgag acagaatact atcatggaga agcaggagag acattttctt tcaataagaa
     6721 aaaaatgatc aatgcatcca tgcaagtaat ggaagaccat ttggagatta agtttgagga
     6781 aggcaagaaa ggtcagccaa agaagctgtt acttgtaaat aagaaaagtc agatcattta
     6841 tacaagggat ctaacagaaa aagaatggaa agatcattgt atcaaggaat attttgatga
     6901 atttctttcg cttgaaattg cagatatgaa cggaagatat cagttgatga tcgaattaga
     6961 agatgaaaat ggtaacaaac tgccatattt ctttgtagat cgtaaagaat atgcaaaacg
     7021 caaagaagtg atggatgaat tagataaatc ttctgtatat atgacaggaa tcaagaaaca
     7081 tgat
//
LOCUS       DAOMUE010000076         7067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2819_length_7067_cov_2.469481, whole genome shotgun sequence.
ACCESSION   DAOMUE010000076 DAOMUE010000000
VERSION     DAOMUE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2819_length_7067_cov_2.469481"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLC71_04835"
     CDS             complement(<1..420)
                     /locus_tag="PLC71_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRM02000.1"
                     /translation="MKIQLSKIISVPKYEETVEASINLNEIKLKGVSYPIREKNDFSI
                     VFRNIGKDKISFAFETEVTLGIPCSRCLEEVSYPVSVQVMKELDFSDLDEEDSSYIED
                     KYLDLDILIFDEVVPKLPSRVLCKEDCKGLCPVCGTNL"
     gene            complement(548..1738)
                     /locus_tag="PLC71_04840"
     CDS             complement(548..1738)
                     /locus_tag="PLC71_04840"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRM02001.1"
                     /translation="MKVLVINCGSSSLKYQLIDSETESVLAKGLCERIGIDGALTHQP
                     AGKDKIKSTPAMPDHKTAIGIVIDQLTDKENGVVESLDEIGAVGHRIVHGGEKFTKSC
                     LITDEVIKEVEECSVFAPLHNPAGLIGVRACQELMPGLPMVAVFDTAFHQTMPEKAFL
                     YGIPRKYYDEDGIRRYGFHGTSHQFVSLETAKVLGKDIKDLKIIVCHLGNGASVTAVK
                     GGESVDTSMGLTPLEGLIMGTRSGDLDPAIIEFIANKENKTASEVLNILNKESGVYGL
                     SGVSSDFRDVKAARAEGNKVAAAALDAYAYRVAKYIGAYTAAMNGVDAIAFTAGLGEN
                     DDITRKAICEYLGYLGTELDDVKNDVHGETAIISTDDSKVTLLCVPTNEELMIARDTV
                     ALVK"
     gene            complement(1765..2778)
                     /gene="pta"
                     /locus_tag="PLC71_04845"
     CDS             complement(1765..2778)
                     /gene="pta"
                     /locus_tag="PLC71_04845"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HRM02002.1"
                     /translation="MSVIDEIKNKAKANKKTVVLPETTDMRTLTAAAEVISEEIADII
                     LVGKEDEIKAKAEAEGLDLAKASFVDPENCDHLNTYIESLCEARKSKGLLPEDAKEIL
                     LSNPLFFGATMVRVGDADGMVAGAINSSANVLRAALQVVKTAPGTEIVSAFMLMDTQA
                     KDMGENGCFVFADCGLNQNPNPEQLAAIASSSAKTFEQLIGATPRVAMLSHSTCGSAK
                     HDDVTKVVEATNIAKEKYPQYLICGELQLDAAIVPEVAASKAPRSEVAGKANVLVFPD
                     LDAGNIGYKLVQRLGGAQAFGPITQGIAKPVNDLSRGCVASDIVGAVAITCVQAAALE
                     ADK"
     gene            3052..4263
                     /locus_tag="PLC71_04850"
     CDS             3052..4263
                     /locus_tag="PLC71_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRM02003.1"
                     /translation="MKTAAIICEYNPFHNGHEYHIMQTRNITGADCVIAIMSGNYVQR
                     GTPAVMDKKIRTKSALLGGADLVIELPLFAACSSAPDFAIGAVSLLHKLGVIDYLSFG
                     SECQDINKLKQIASFLIQYEEEIERGTKELMSLGHSYPKAKELYLREHLDDASLIEVL
                     KQPNNILGIEYLKALLQLDSPIIPVCVTRVSNDHHSKELTPSISSATSIREILEQNQI
                     SPLFSRVPEALHDIYQMHYQKDFPVCLDDFSLLLHAAIYSCEDLTQISGISSDFKDRI
                     FKMLKSDLSFEELISACKTKNLTWSKISRNLLHIMLDITKEKVEKARTYDMSPYFQIL
                     GFKRSASFLIGNIKRKSSIPMVRHLRVMNDPLSKDQEELLSTEQRANQLYRTVIGQKF
                     HTIIKDEQIIF"
     gene            4312..>5048
                     /locus_tag="PLC71_04855"
     CDS             4312..>5048
                     /locus_tag="PLC71_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02004.1"
                     /translation="MKTSFIQFLLIFSVLSLLFFPQTSVQGAKNGLILWSATITPTLL
                     PFLLLTGFMQYYQTFHLISRLFYPLKKLFPGINEDFFYTCILGFFCGCPLGAKIIDDF
                     ISCGSFTKSEGEKLLYTCNQISPMFSAGYTLTLILHGKISALRFFFCLYFPVMCYLIY
                     LVFLFFTSDFLHTHHSSPTIAHKKSADQVIFDSLHSIFMIGICIMIFSIAVSLLEILP
                     LPFYLKRGLTAVLEITNAISYFGSSHMS"
     assembly_gap    5049..5064
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5154..5762)
                     /locus_tag="PLC71_04860"
     CDS             complement(5154..5762)
                     /locus_tag="PLC71_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02005.1"
                     /translation="MSEKYLHEMIDDIEVLIDEAKPARFSPGKIMIEKDVLMTVIEEL
                     RKQIPGEIERSHKIILNKDAILEDARVKSQQMIDQAARDAGELIDQNEVVEMAKMRAN
                     EIETHAKDTARSVVHNANEEADQIRAGALRYVNEIMEDVQGYVASVKEAQESVFNQLL
                     SALDSDLASIKTNAKEIEEQLSGVSIPQARTRTVEDFISDED"
     gene            complement(5776..6255)
                     /gene="coaD"
                     /locus_tag="PLC71_04865"
     CDS             complement(5776..6255)
                     /gene="coaD"
                     /locus_tag="PLC71_04865"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRM02006.1"
                     /translation="MSIAVYPGSFDPVTYGHIDIIERSAKVFDKLIIAVLVNSAKKPM
                     FTTQEKVDMIRKVTSHLDNVEVMSFSGLLVDFAKQQGASVSVRGLRAVTDFEYEIQIA
                     QTNARLDKDLDTMFFTTSIEYSYVSSTIVKEIASYHGDVSEMVPPYVQECLEKKFKS"
     gene            complement(6259..6816)
                     /gene="rsmD"
                     /locus_tag="PLC71_04870"
     CDS             complement(6259..6816)
                     /gene="rsmD"
                     /locus_tag="PLC71_04870"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRM02007.1"
                     /translation="MRVVAGSAKSLKLKTIEGMETRPTQDRIKETLFNMIQYDIPGST
                     FLDLFSGSGGIGIEALSRGAKEAYFVEKAKPALRCIRENLRYTKLDKKAQVLATDVNS
                     AIRQLETKNVTFDHIFMDPPYKKGFEEEVLSIIDRSSICTEDTVVIVESSLDTEIPDF
                     EKLKVIRVKEYKTNKHTFLMKTVED"
BASE COUNT         1863 a         1575 c         1117 g         2496 t
ORIGIN      
        1 cagattcgtt ccacataccg gacaaagtcc tttgcagtct tctttgcata acactctgga
       61 cggaagtttt ggaacaactt catcgaagat cagtatatca agatcaagat acttgtcctc
      121 gatatatgaa gaatcttctt catcgagatc cgaaaaatct aattctttca tcacctgcac
      181 ggaaaccgga taactcactt cttcaagaca tcggctgcat ggaattccaa gcgtgacttc
      241 tgtctcaaat gcaaatgata tcttatcctt tccaatattt ctaaaaacaa tcgaaaaatc
      301 attcttctct cgaataggat aagacacacc ttttagctta atctcattca gattgattga
      361 cgcttcgacg gtttcttcat atttaggtac tgaaataatt tttgataatt gaatcttcat
      421 acgatctcct aaattttcat actgtactat tatacgaatg tgcttctggt ttgtcaagta
      481 attttcctta cttttaaaaa aacaatccag aagcacattt taacaagctt tattttttat
      541 gaaatgatta tttgacaagg gctactgtgt ctcttgcgat cattaattct tcatttgttg
      601 gtacacaaag cagtgtaact ttagaatcat ctgtagagat gatcgctgtt tctccgtgaa
      661 catcgttctt cacatcgtca agttctgttc ctaaatatcc aagatattcg cagattgctt
      721 tacgtgtgat atcatcattc tcaccaagtc ctgctgtaaa tgcgatcgca tcaacaccat
      781 tcatagctgc tgtgtaagct ccgatgtatt ttgctacgcg gtatgcatat gcatctaatg
      841 ctgcagctgc aactttgtta ccttcagctc ttgctgcttt tacatctcta aagtcagaag
      901 atactcctga taatccgtaa actcctgatt ctttgtttaa gatatttaat acttcagatg
      961 ctgttttgtt ttctttattc gcaataaatt cgatgattgc tggatcaaga tctccagaac
     1021 gtgttcccat gatcagtcct tcaagtggtg ttaatcccat agatgtatct actgattctc
     1081 cacctttgac tgctgtaaca cttgcaccgt ttcctaaatg gcatacgatg atctttaagt
     1141 ctttgatatc ttttcctaag acttttgctg tctctaaaga tacgaactga tgagatgttc
     1201 cgtggaatcc ataacggcgg ataccatctt catcgtagta ttttctagga ataccatata
     1261 agaatgcttt ctctggcatt gtctgatgga atgctgtatc aaatactgct accattggaa
     1321 gtcctggcat taattcctga catgcacgaa ctccgatcag tcccgctggg ttatgaagtg
     1381 gtgcgaatac gctgcattct tcaacttctt tgataacttc atctgtgatc aagcatgatt
     1441 ttgtgaattt ctctccaccg tgaacaatac gatgtcctac agctccgatc tcatcaagac
     1501 tttctacaac tccgttttct ttatctgtca gctggtcgat aacgattccg atcgctgtct
     1561 tatgatctgg catagctggt gtagatttga tcttgtcttt tcccgctggc tgatgtgtta
     1621 atgctccatc aattccgatt ctttcacata atccttttgc taaaacactt tctgtctctg
     1681 aatcgattaa ctgatatttt aatgatgagc ttccgcagtt gataactaaa actttcatga
     1741 actatgattc tccttatatt tttcttattt atctgcttcc agtgctgctg cctgtacaca
     1801 tgtgatcgct actgcaccta cgatatctga tgctacacat ccacgtgata agtcattgac
     1861 tggttttgcg attccctgtg taataggtcc gaatgcctgc gctccaccta atctctgcac
     1921 taatttgtat ccaatgttac cagcatctaa gtctgggaat actaatacgt tcgctttacc
     1981 agcaacttca cttcttggag ccttacttgc tgcaacttct ggtacgatcg ctgcgtcaag
     2041 ctgtaattct ccacagatca ggtactgagg gtatttttct ttagcgatgt ttgttgcttc
     2101 tacaactttt gttacatcgt catgttttgc acttccacat gtagaatggg ataacattgc
     2161 aactctaggt gttgctccga tcaactgctc aaatgtctta gctgagcttg aagcgattgc
     2221 tgctaactgt tctgggtttg gattctggtt cagtccacag tcagcgaata cgaaacatcc
     2281 gttttcaccc atatctttag cctgtgtatc cattaacatg aaggctgata caatctctgt
     2341 accaggtgca gtcttaacaa cctgaagtgc tgcacgaagt acgtttgcag aagagttgat
     2401 cgctcctgct accattccgt ctgcatctcc aactcttacc attgttgctc cgaagaacag
     2461 tgggttggat aacaggatct cttttgcatc ttctggaagc agtcctttgc tctttcttgc
     2521 ttcacataaa gattcgatat atgtatttaa atgatcacag ttctctggat caacaaaaga
     2581 agctttggct aaatctaatc cttctgcttc tgcttttgcc ttaatctcgt cttcttttcc
     2641 aactaaaata atatcagcga tctcttcact gataacctct gctgctgctg ttaatgttct
     2701 catgtctgta gtttcaggca gtacaacagt tttcttattt gcttttgcct tgtttttaat
     2761 ctcatcaata acactcattt ttcgcgttgc tcctttcaac tttccaaaaa gatgaattta
     2821 atcttgttaa attatacaac atcatttctt tgtatacaat aatagtattt gtatttttcg
     2881 ttgtacattt ccagaaaaat ctatattcta ttctacattt ttttgttttc tctgacaaga
     2941 tacttgttgc tcatttttca ttttttgtgc aattttactt ttattcattt gtgatttata
     3001 atacatttac aaaataaacg attcaaataa ggaatctcga aaggatattt tatgaaaaca
     3061 gcagctatca tttgcgaata caatccgttt cacaatggtc atgaatacca catcatgcag
     3121 acaaggaata tcacgggtgc agactgcgtc attgccatca tgagtggaaa ctatgtacag
     3181 cgcggaactc cggcggttat ggacaaaaaa atacgtacca agtctgccct tcttggaggt
     3241 gccgatctcg tgatcgaact ccccttattt gcagcatgtt catctgcccc tgattttgca
     3301 attggagctg tcagtctgct tcacaaacta ggggtgatcg actacctttc ttttggaagt
     3361 gagtgtcaag atattaacaa acttaagcag attgcatcct ttcttatcca atatgaagaa
     3421 gaaatcgagc gtggtacaaa agagcttatg agtctcggac attcttatcc gaaagcaaaa
     3481 gaactttatc ttagagagca tcttgatgat gcttctttga tcgaagtatt aaaacagccg
     3541 aataatattc ttgggattga atatttaaaa gctttgctac agttagacag ccctatcatt
     3601 cccgtctgtg ttacccgtgt ttcaaacgat catcacagca aggagttgac accttcgatt
     3661 tcttctgcaa catccatccg tgaaatctta gaacaaaacc agataagccc tttattctca
     3721 agagtccctg aagctttgca tgatatctat cagatgcatt atcaaaaaga ttttcctgtc
     3781 tgcctggatg atttttctct gcttttacat gcagctattt attcttgcga agatcttaca
     3841 cagatcagcg gtatttcaag cgattttaag gaccgtattt ttaagatgtt aaaatctgat
     3901 ctttcttttg aagaactgat cagtgcctgt aagaccaaaa acctcacctg gagcaaaata
     3961 agcagaaatc ttttgcatat catgcttgat ataacaaaag aaaaggttga aaaagcacga
     4021 acttacgata tgtctcctta ttttcagatt ctaggtttca aacgttctgc ttccttcctg
     4081 atcgggaata tcaaaagaaa atcttccatc cctatggtac gccatctgcg tgttatgaat
     4141 gatcctttat ccaaagatca agaagaatta ttatctacag aacaacgtgc caaccagtta
     4201 tatcgtaccg tgatcggaca gaaatttcat acgatcataa aagatgagca gatcatcttc
     4261 tagtttcacc tcccgcttca tacactataa aaagaaagcg ggagattttt catgaaaacc
     4321 agttttatcc agtttttact tatttttagt gttctttcct tgttattttt tccacaaaca
     4381 tcggtccaag gagcaaaaaa tgggctgatt ttatggtctg caacgatcac accgactctt
     4441 ttaccttttt tactgctcac tggttttatg caatattacc aaacgttcca tctgatctcc
     4501 cggctctttt accccctcaa aaaacttttt cctggtatca atgaggactt tttttacacc
     4561 tgtattctag gttttttttg tggatgtccc ctcggtgcga agatcattga tgattttatt
     4621 tcttgtggat cttttacgaa atctgaagga gaaaagctat tatatacctg caatcagatc
     4681 agtcccatgt tctctgctgg atatactctt acattgatcc ttcatggcaa aatttctgct
     4741 ctgcgctttt tcttttgcct gtattttcct gtcatgtgtt atctgatcta tctggtcttt
     4801 ttatttttta caagcgattt tctacataca caccactctt ctcccaccat tgcacacaaa
     4861 aaatcagcag atcaggtaat ctttgatagt cttcattcca ttttcatgat cggaatctgt
     4921 attatgatct tttcaattgc cgtttctctt ttagaaattc ttccacttcc tttctattta
     4981 aaaagaggat tgaccgctgt tcttgaaatc acaaatgcca tctcctattt tggttcttcc
     5041 catatgtcnn nnnnnnnnnn nnnntaagtg tatacaaaaa gagcagactc tccctctgga
     5101 aatatctgct cataagatct ctttttagtg tttcaagcgg actacttgct tatctagtct
     5161 tcatctgaaa tgaaatcttc taccgttctt gttcttgcct gtggaatgct gactccggaa
     5221 agctgttctt ctatctcctt cgcattcgtt ttaatcgatg caagatctga atctaatgcg
     5281 gataacaact ggttaaacac actctcctgt gcttctttga cacttgccac ataaccttgt
     5341 acatcttcca tgatctcatt gacatagcgc aatgcacctg cacggatctg atctgcttct
     5401 tcattcgcat tatgtacaac acttcttgct gtatctttgg cgtgagtttc aatctcattc
     5461 gctctcatct ttgccatctc aacgacctca ttctgatcga tcaattctcc tgcatctctt
     5521 gctgcctgat cgatcatctg ctgtgacttc actctggcat cttccaagat cgcatctttg
     5581 ttcagaatga tcttatggct tcgttcaatc tcacctggaa tctgcttcct taattcctcg
     5641 atcactgtca tcaatacatc tttctcgatc ataatcttac ctggactaaa tcttgctggt
     5701 tttgcttcgt cgatcagtac ttcaatatca tcaatcatct catgtaaata cttctcactc
     5761 atattcattc gcctcctatg atttaaattt cttctctaga cattcttgta cgtaaggcgg
     5821 taccatctca gaaacatctc catgataaga tgcaatttcc tttacgatcg tggaacttac
     5881 ataagaatac tcaatgctgg ttgtaaaaaa catggtatct aagtctttat ccaatctcgc
     5941 atttgtctgt gcaatttgaa tctcatactc aaaatcagtt actgcgcgta gtcctcggac
     6001 tgatacactg gctccctgtt gttttgcaaa gtctaccaac aatccagaaa atgacataac
     6061 ctctacatta tcaagatgac ttgtcacttt tcgtatcata tcaaccttct cttgtgttgt
     6121 aaacattggt ttctttgcac tgtttaccaa tactgcgatg atcagcttgt caaaaacctt
     6181 ggctgaacgc tctataatat caatatgtcc ataagtcacc gggtcaaaac ttcccggata
     6241 aactgcaata ctcatcttct aatcctccac tgttttcatc aaaaatgtat gtttatttgt
     6301 cttgtattct tttactctta taacctttaa tttctcaaaa tctggtatct ctgtgtcaag
     6361 agacgattcc acgataacta ccgtatcttc tgtacagata gaggatcgat ctatgattga
     6421 taatacttct tcttcaaatc ctttcttata tggaggatcc ataaaaatat gatcaaatgt
     6481 tacattcttc gtctctaact gcctgatcgc tgaattcaca tctgttgcca gaacctgtgc
     6541 tttcttatca agctttgtat atcttaaatt ctctcgaatg catcttaatg caggttttgc
     6601 tttttcaaca aaataagctt cctttgctcc tctgctcaat gcctcgatcc cgattcctcc
     6661 acttccagag aaaagatcca gaaatgtact tcccgggata tcatactgaa tcatattaaa
     6721 taacgtctct ttgatgcgat cctgggttgg tcttgtctcc attccttcga tcgtctttaa
     6781 ttttaaactc ttcgcacttc ctgctacgac tctcatatct atgtatattc cttgtctttt
     6841 atttgctttg caaagttatt ccacttttgc tcactatttc ttttatttta ttatatctac
     6901 agattttgtc aaccgacaaa ccaatatttc agaaagagac agtcaaagtt tatactttca
     6961 ctgcctcttt ctgaaatctt atgatgtttg aggattgctt cattgctacg caatttcgca
     7021 atccggatac atcatcttat ctaattagcg agacatttct tcttcct
//
LOCUS       DAOMUE010000077         7009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2854_length_7009_cov_2.117199, whole genome shotgun sequence.
ACCESSION   DAOMUE010000077 DAOMUE010000000
VERSION     DAOMUE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2854_length_7009_cov_2.117199"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..554
                     /locus_tag="PLC71_04875"
     CDS             270..554
                     /locus_tag="PLC71_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HRM02008.1"
                     /translation="MGFAETFKALSDPIRREILMMLRDGSMSAGEIGSHFGMTGATIS
                     YHLKILKKAELIFEKKEKNYIYYTLNTSVVEEVMLWLSELKGGNHDVKEE"
     gene            535..1191
                     /locus_tag="PLC71_04880"
     CDS             535..1191
                     /locus_tag="PLC71_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HRM02009.1"
                     /translation="MMLKKNKKFFIASCIVILLPMFAGFLLWDQLPAQVATHFGRNGE
                     ADRYSSKLFAVAGIYLFVLAVHILCSVITEFDPKKRNVGSKIYHLILCICPVVSLWCG
                     VMIYGNALGYKDVINLNLWTNVLLGIVLIVVGNYLPKCRQNYMIGIKLPWTLDNEENW
                     DYTHRMAGKWWIIGGVLTILLGFQKFVDPIGAAVGIILIVTLIPAIASYIYYKKHRVM
                     "
     gene            1352..1489
                     /locus_tag="PLC71_04885"
     CDS             1352..1489
                     /locus_tag="PLC71_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02010.1"
                     /translation="MENLLYGKQIEEKHYDAQLLAKGMKKEKIRHYGFAFCGKKVLIG
                     K"
     gene            1666..4080
                     /locus_tag="PLC71_04890"
     CDS             1666..4080
                     /locus_tag="PLC71_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HRM02011.1"
                     /translation="MNNTEHFGKLTERMQAFREEVLDAKPYIDAQRAVLATEAYKENQ
                     NQPRVMVRALMLQKILEGMSIYIDDKSLIAGNQATKNKNAPIFPEYTMEFVMNELDLF
                     EKRDGDVFYITEETKEQLREIAPFWENNNLRARGEALLPEEVDVFMETGVFGMEGKLN
                     AGDAHLAVNYQRILSDGLKGYEKRVKELRAALDFTDPESIDKNVFYKAVLTVIEAVRD
                     FAQRYSKLAKELADKETDAKRKEELLQMSKICAKVPYEPANSFREAVQSVWFIQLILQ
                     IESNGHSLSYGRFDQYMYPYYMKDINEGKITKEDALELLTCLWIKTLTINKVRSQSHT
                     LSSAGSPMYQNVTIGGQTTDKKDAVNELSFVVLQSVAQTRLTQPNLTVRYHANIDKHF
                     FDECIEVMKLGFGMPALNNDEIIIPSFINWGVKEEDAYNYSAIGCVETAVPGKWGYRC
                     TGMSYINFPRVLLCAMNDGVDLTSGKRFTKGYGKFTEMETYEDLLAAWDKTVREMTRY
                     SVIVENAIDKASERDVPDVLCSALTDDCIGRGKTIKEGGAVYDFISGLQVGIANMADS
                     LAAIKKLVYEEKKITKEQLWNAILDDFQSEENKKIQEMLINDVPKYGNDDDYVDQLVV
                     EAYDSYLDEIKKYPNTRYHRGPIGGIRYGGTSSISANVGQGMGTIATPDGRNAFEPLA
                     EGSSPAHNADKNGPTAVFKTVAKLPTEKITGGVLLNQKMTPQMLSTEENKQKLEMLIR
                     TFFNRLHGYHVQYNIVSRETLIDAQKHPEKHKDLIVRVAGYSAFFNVLSKKTQDDIIG
                     RTEQTL"
     gene            complement(4240..5142)
                     /locus_tag="PLC71_04895"
     CDS             complement(4240..5142)
                     /locus_tag="PLC71_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HRM02012.1"
                     /translation="MQTNGIVFNIQKFSIHDGPGIRTTVFLKGCPLRCKWCANPESQL
                     SQVQILYDKEKCAHCGTCTHVCPNQAISMGDDSYVGIDPSKCAGCLQCVKNCPAKALS
                     YEGEAKDVDEVVKVCLQDIDFYEESGGGVTISGGEGMVQPDFVKALLAKLKEHKIHTA
                     IETTGYIKKEIFRELAPMFDLLLFDVKHYDSDKHYEGTHVHNELIIENLAWAISQGIE
                     VLPRIPVIPDFNDSLDDAKGIAALLNQVGAKKVQLLPFHQFGENKYHLLGKTYSYENV
                     KALHPEDLTDYQQIFLDVGIDCFF"
     gene            5332..6012
                     /locus_tag="PLC71_04900"
     CDS             5332..6012
                     /locus_tag="PLC71_04900"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRM02013.1"
                     /translation="MEFILDTVDLEEIKDGVDHMPIVGVTSNPSIVKKTSPEDFFGHM
                     RKVREIIGKERSLHVQVISLDAETIVKEAHRILEEIDDQVYIKVPVSYEGLKAIKILK
                     SEGVNVTATAVYDLMQAYFALAAGADYIAPYVNRIGNLGADPMELISNLQDRIEDDGY
                     DCKIVAASFKGVEQVKNAFNYGAEAITAPYAVLKQVFANPNIDKAVTDFNNDWYAVYG
                     EGKGICDL"
     assembly_gap    6152..6161
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6162..6828
                     /locus_tag="PLC71_04905"
     CDS             <6162..6828
                     /locus_tag="PLC71_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRM02014.1"
                     /translation="VTIRKDLDALEELNFLTREHGYATMKNMSDISNRMAFCYETKQR
                     IAEKASEIISDGETIMVESGSSCALLVKYLSETKKDITVITNSSYIARFIRETEGGVT
                     KVIVLGGEYQKEAEVMVGPLVKTCAEAFYVDKLFMGTDGYIPGIGFTSGDMLRAEAAK
                     SMADSTRNSIILTDSSKFSEHGVVMQCRLKDINMVFTDDQVPLEAKDAMEQNGIIVET
                     VPR"
BASE COUNT         2499 a         1092 c         1465 g         1943 t
ORIGIN      
        1 gcattgatcg ttcggtcaga tatatatcct ttgtcattta ataaagttcc atccatatcg
       61 gatgcaaata atcgtatcat attaagtact cctttcgtgt gtataagatg atggtaacag
      121 ggaaatacaa aatttaaatg atatctatgt taaatttctg caatgaaaac tatttagaaa
      181 aatatctaaa tagtaattga cttttggaat atatcatgct ataaagtaat tagaaatatt
      241 tctaaatagt cacagaaagg atgagagtta tgggatttgc agagacattt aaagcattat
      301 cagacccgat acgaagagag attcttatga tgttgcggga tggtagtatg tccgctggag
      361 agattggttc tcactttggt atgacaggtg caaccatttc ttatcatttg aagattctta
      421 aaaaagcaga gctgatcttc gagaagaaag agaaaaatta tatttactac acgttaaata
      481 catcagttgt tgaggaagtt atgttatggt tgtcagaatt gaagggagga aatcatgatg
      541 ttaaagaaga ataagaagtt ttttattgca agttgtattg taattttatt accaatgttt
      601 gctggattct tattatggga tcagttgcca gcacaggtgg caacacattt tggaagaaac
      661 ggcgaggccg atcgatacag cagcaaatta tttgcagtag caggaattta tctgtttgtt
      721 ttggcagtgc atattttatg tagtgttatc acagaatttg atcctaagaa aaggaatgtt
      781 gggtctaaga tttatcatct gatcctatgt atctgtccgg ttgtatcact ttggtgtggg
      841 gtaatgatct atggaaatgc attaggatat aaagacgtga tcaatttaaa tctgtggacg
      901 aatgttttac taggaatagt tctgatcgtt gttggaaact atcttccaaa gtgcagacag
      961 aactatatga tcggaatcaa acttccatgg acattggata atgaagaaaa ctgggattac
     1021 acacatcgaa tggcgggaaa atggtggatc atcggtggag ttttgacgat tctgcttgga
     1081 tttcagaagt ttgttgatcc aattggagca gcagtaggga ttattttgat cgttacattg
     1141 attcctgcga ttgcctctta tatttattat aagaagcata gagttatgta aaaaagcatt
     1201 gtggtagata aaaagaaatc agatgttata ataaagttaa ttatcggaca gaagggagtg
     1261 atcacatgca acaagtaata tctgtaggga atcaggattt taaatcaatc agagaaagag
     1321 caggcaaagt aattcggata ttaaagttat catggaaaat ttgctttatg gaaagcagat
     1381 agaagaaaaa cattatgatg cacaattgct tgcaaaagga atgaaaaaag aaaagatcag
     1441 acattatggt tttgcctttt gtggaaagaa agtactgatc ggaaaataag aaaagatgtc
     1501 ataatagaat atggcatctt ttcttttaca caaaaaagaa gtttcaaatg aagataaaaa
     1561 taattatgaa ccaaatctat tgacatacaa acaaaaaaat gctaacttat aattacaaac
     1621 aaaacaaaaa taagttacga acgaaacatt ctgaaaggag ataccatgaa caacacagaa
     1681 catttcggta aattaacaga acgcatgcag gcatttcgtg aggaagtgct cgatgcaaaa
     1741 ccatatattg acgcacagag agccgtactg gcaacagaag cttataaaga aaaccagaat
     1801 cagccaagag ttatggtaag agctttaatg ctacagaaga tcttagaagg aatgagcatt
     1861 tacattgatg acaaatccct gatcgcagga aatcaggcaa caaagaacaa gaatgctcca
     1921 atcttcccgg aatataccat ggaatttgtg atgaacgaat tagatctctt tgagaaacgt
     1981 gatggagatg ttttctatat tacagaagag acaaaagaac agttaagaga gattgcaccg
     2041 ttctgggaga ataacaatct tcgtgccaga ggagaagctt tacttccaga agaggtagat
     2101 gtctttatgg agacaggtgt ctttggaatg gaaggaaaat taaatgctgg agatgcccat
     2161 ctggcagtga attaccagag aatcttatct gatggattga aaggatatga aaaaagagta
     2221 aaagaattaa gagcagcatt agattttaca gatccagaaa gcattgataa gaacgtattt
     2281 tataaagcag tcttaacagt cattgaagcc gttcgtgatt ttgcacagcg ttacagcaaa
     2341 cttgcaaaag aattagcaga caaagaaact gatgcaaaac gtaaagaaga attattacag
     2401 atgagcaaga tctgtgccaa ggttccatat gaaccagcaa attcctttag ggaagctgta
     2461 cagtctgttt ggtttatcca gttgatcctt cagatcgaat ctaacggaca ttccttaagt
     2521 tatggacgtt ttgaccagta tatgtatcca tattatatga aagatatcaa tgaaggaaag
     2581 atcacaaaag aagatgcatt agaattatta acatgtcttt ggatcaagac attaacgatc
     2641 aataaagtac gttcccagtc acatacatta agttccgcag gatcaccaat gtaccaaaac
     2701 gtaacgatcg gtggacagac aactgataag aaagatgctg tcaatgaatt aagttttgta
     2761 gtattacaat ccgttgcaca gacaagactg acacagccaa acttgacagt ccgttatcat
     2821 gctaatatcg acaaacattt ctttgatgaa tgtatcgaag ttatgaaact cggatttggt
     2881 atgccagcat taaataacga tgagatcatc atcccatctt tcatcaattg gggtgtcaaa
     2941 gaagaagatg catacaatta tagtgcgatc ggatgtgtag agacagctgt tccaggtaaa
     3001 tggggatatc gttgtaccgg tatgtcttat attaacttcc caagagttct gctttgtgcg
     3061 atgaatgatg gagttgactt aacaagcggt aagagattta ccaaaggata tggtaaattt
     3121 acagagatgg aaacatatga agatctctta gcagcatggg ataagaccgt cagagaaatg
     3181 acacgttata gtgttattgt ggaaaatgca attgataaag catctgagcg agatgtacca
     3241 gacgttttat gttctgcatt aacagatgac tgtattggaa gaggaaaaac gatcaaagaa
     3301 ggtggagcag tctatgactt tatctctgga cttcaggttg gtattgctaa tatggcagac
     3361 tctcttgcag cgatcaagaa acttgtatat gaagagaaga aaatcacaaa agaacagctt
     3421 tggaatgcaa tcttagacga tttccagtca gaagagaaca agaagattca ggaaatgctc
     3481 attaatgacg ttccaaaata cggaaatgat gatgactatg tcgatcagtt agtcgtagaa
     3541 gcatacgatt cttacttaga tgagattaag aaatatccaa acacacgtta ccacagagga
     3601 ccgatcggcg gtattcgtta tggtggaaca tcatctatca gtgcgaatgt tggacaggga
     3661 atgggaacca ttgcgactcc agatggaaga aatgcatttg aaccattagc cgaaggaagc
     3721 agtccggcac ataatgcaga taagaatggt ccaacagcag tatttaagac ggttgcgaaa
     3781 cttccaacag agaagatcac aggtggagtt ttattaaacc agaagatgac accacagatg
     3841 ttatcaacag aagaaaacaa acagaaatta gagatgttga tccgtacatt cttcaaccgt
     3901 ttacatggat atcatgtaca gtataatatt gtatccagag aaactctgat cgatgcccag
     3961 aaacatccag agaaacataa agacctgatc gttcgtgtgg caggatacag tgcattcttt
     4021 aatgtattat ctaagaagac acaggatgat atcatcggaa gaacagagca gacattataa
     4081 tttacaaaaa tagaggaatg gcagttggct tgagtagggt taactgctgt ttttcgtgat
     4141 agactagaag aaataatatt catgaaagaa acaattaaaa aagccttggc aagaaactgt
     4201 tctgccaaag ctttttgcgt atatggaatt ttcaaaaggt tagaaaaagc aatcgatacc
     4261 tacatctaaa aagatctgct gataatcggt aagatcctca ggatgcaatg ctttcacatt
     4321 ctcataagaa taagtctttc cgagtaaatg atacttgttt tctccgaatt gatggaaagg
     4381 aagcagttga accttcttcg ctccaacctg atttaataaa gcagcgattc cttttgcatc
     4441 atccaaagaa tcattgaaat caggaatcac agggattcga ggaagaactt caattccttg
     4501 agaaatcgcc catgctaaat tctcaatgat cagttcgtta tggacatggg ttccttcata
     4561 atgcttatca ctgtcatagt gctttacgtc aaacaaaagc aagtcaaaca tcggagccaa
     4621 ttctctaaaa atctctttct tgatatatcc agttgtctca atcgcagtat gaatcttatg
     4681 ttcttttaat tttgcaagta aagcctttac aaaatctggt tgaaccattc cttcaccgcc
     4741 agagattgtt acaccgcctc cagattcctc ataaaaatca atatcctgaa gacagacttt
     4801 gacaacttca tctacatctt ttgcttctcc ttcataactt aaggcctttg caggacagtt
     4861 ttttacacat tgaagacatc cagcgcattt tgatggatca atacctacat aactgtcatc
     4921 tcccatagaa attgcctggt ttggacagac atgggtgcaa gtaccgcaat gagcacattt
     4981 ttctttatca tataaaattt ggacttggct caactgtgat tccggatttg cacaccattt
     5041 acaacgcagt ggacagcctt ttaggaaaac tgttgtacgg attcctggtc catcgtgaat
     5101 gctgaatttc tgtatattaa aaacaatacc atttgtttgc atggataata tttctccttt
     5161 ctgattcttt tgtatatctt atcacacaac ttacgaaaag taaataatat acttacaaat
     5221 gaaagttaaa aaacattaaa cgaaaataat tgttgacata ttcgaaaatt atggtaagat
     5281 agaagtatca aaaaacgaaa cacgtaataa gacttaggag gagttttaat catggaattt
     5341 atcttagata cagtagatct tgaagagatc aaagacggtg tagaccacat gccaatcgtt
     5401 ggagtaacaa gcaacccatc catcgttaag aaaacatcac cagaagattt ctttggacat
     5461 atgagaaagg ttcgtgagat catcgggaaa gaaagaagcc ttcacgtaca ggtaatttca
     5521 ttagatgcag aaacaatcgt taaggaagca cacagaatct tagaagagat tgatgatcag
     5581 gtatatatca aagttcctgt atcctatgaa ggattaaaag cgatcaagat cttgaaatca
     5641 gaaggtgtta acgtaacagc aacagcagtt tatgatctaa tgcaggcata ttttgcatta
     5701 gcagcaggag ctgactatat cgcaccatat gtaaatcgta tcggaaatct cggggcagac
     5761 cctatggaac tgatcagcaa tcttcaggac cgtatcgaag atgatggata tgattgtaag
     5821 atcgttgcag catcatttaa aggcgttgaa caggtgaaga acgcatttaa ctatggagca
     5881 gaagcaatca cagcaccata tgcagtatta aaacaggtat ttgcaaatcc taatattgac
     5941 aaagcagtga cagacttcaa caatgactgg tatgcagttt atggagaagg taaaggaatc
     6001 tgtgaccttt aattaaaaat atgctattat atgaatagtg cagagggata aagagccgca
     6061 agacggctct ttcctaatat tatgaagaaa gggaaagttt catgacagac agacaaacaa
     6121 agattcttga aatcgtaaat caaaaaaaga annnnnnnnn nggtcacgat cagaaaagat
     6181 cttgatgctt tggaagaact gaattttctg acaagagaac atggatatgc gacaatgaag
     6241 aatatgtcag acatcagcaa tcgtatggct ttttgttatg agacgaaaca gaggatcgca
     6301 gaaaaggcaa gtgagatcat atctgatgga gaaacgatca tggtggaatc tgggtcaagt
     6361 tgtgcattgc ttgtaaaata cctttcagag actaagaaag atattacagt tatcacgaat
     6421 tcatcctata ttgcccgatt tatcagagaa acagagggcg gtgttacaaa ggttatcgtt
     6481 cttggagggg aatatcagaa ggaagcagaa gttatggtgg gaccattggt gaaaacttgt
     6541 gcagaagctt tttatgtaga taaactattt atgggaacgg atgggtatat ccctggcatc
     6601 ggattcacaa gcggagatat gttaagggcc gaagctgcca agagtatggc ggattctaca
     6661 aggaattcta tcattttaac agattccagc aagttttcag aacatggagt tgtcatgcag
     6721 tgtagactca aagatattaa tatggtattt acggacgatc aagtgccatt agaagctaaa
     6781 gatgcgatgg aacaaaatgg gattatcgta gaaacagtac ctagataaga aagaaatgat
     6841 aatagaaact gtcatatgaa tggaagaaaa attcgtatgg cagttttcgt tttcacagta
     6901 aaaacactgc tgttaatatg tacaaaaata aatacataag atataataaa gttgtaattt
     6961 aataattaaa acaaaaggag gcagtggatg agaacaagaa agatgatgt
//
LOCUS       DAOMUE010000078         6991 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2862_length_6991_cov_2.574539, whole genome shotgun sequence.
ACCESSION   DAOMUE010000078 DAOMUE010000000
VERSION     DAOMUE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2862_length_6991_cov_2.574539"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLC71_04910"
     CDS             complement(<1..426)
                     /locus_tag="PLC71_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRM02015.1"
                     /translation="MDILVCIKQVPASNKVEVDPVTGVLLRNGADSKMNPYDLYALET
                     ALKIREEVGGTISVISMGPPQAMTVIREAFAMGVDRGALLSDRRFGGADVLATSYTIA
                     QGIKKMGDFDLILCGKQTTDGDTAQVGSEVSEWLNIPSVS"
     gene            complement(683..1372)
                     /locus_tag="PLC71_04915"
     CDS             complement(683..1372)
                     /locus_tag="PLC71_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRM02016.1"
                     /translation="MEKTKKAYEIVIDSIKEQIMNQELVLGQGLPPEREISEKLGVSR
                     NSVREALKILTVMGVVSSRQGAGNYISGNFKGYMVDALSMMFMLDKLDYDQINQIRIG
                     LEMQAYALAVKNAETKDIIELQEYVNALDQSTDNDEKTMYDKKIHYAIAKASGNVLIV
                     NILDALSEVMDIFIFEMRREILRTEKRKGMLQEAHKQIVEYLLKRDVVNGQKALMKHF
                     EMIDNIIHKEL"
     gene            1561..2182
                     /locus_tag="PLC71_04920"
                     /pseudo
     CDS             1561..2182
                     /locus_tag="PLC71_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530708.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
     gene            complement(2165..2440)
                     /locus_tag="PLC71_04925"
     CDS             complement(2165..2440)
                     /locus_tag="PLC71_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRM02017.1"
                     /translation="MKNVTYILKCSDNSLYTGWTNDIKHRLKMHNEGKGAKYTRGRGP
                     VELVYLEEFDTKQEAMSKEAKIKRLTRKEKLLLIENYQQEQKLNLEH"
     gene            complement(2580..>2740)
                     /locus_tag="PLC71_04930"
     CDS             complement(2580..>2740)
                     /locus_tag="PLC71_04930"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRM02018.1"
                     /translation="KDIRVKEGTMVNAGEVIAKVAEPTSYYTLEGTNLYFQMEKDKKS
                     VDPLKYLE"
     assembly_gap    2741..2750
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2751..3278)
                     /locus_tag="PLC71_04935"
     CDS             complement(<2751..3278)
                     /locus_tag="PLC71_04935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRM02019.1"
                     /translation="MRKNNKQNPFPLRKYYGAMIIGFVFFLGIMAYYSATFRQRRDQA
                     KQEVSLEEESIGANNSATQEKTKTTTQDKKETETTAQMKRTMSESVTYNGTSKLTWPI
                     KGNIILPYSVNSTIYFESLDQYRINKGILIEAKEGDEVKAVRKAKVREIKKSAEYGQT
                     VLMDLGNEYTVLYGQL"
     gene            complement(3319..4251)
                     /locus_tag="PLC71_04940"
     CDS             complement(3319..4251)
                     /locus_tag="PLC71_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HRM02020.1"
                     /translation="MRYKMTYILFLFFLLTTIPYIITIYINKENKKNISFENYDSGYK
                     IQDKDQDIDLESYLLKILPGQISMDQEEETIKCQAVILRTDLIRKMGRSKKIKMESIP
                     YQVYKDEQYKSKLGDRAYEIMDQKRKKAVKETIGKVITYKGALIEPYFHAVSVGMTLD
                     ASEWFGKKIPYLRQKESLSDIESKDYMSIKTISYQRMQIILEEHMKKKITIQQLQKNL
                     RLLTVTKNGYVKQIKAGSCIISGEDFARWLQLASNNFYFEQYKGNLRIICLGKGNGLG
                     LSQYGADQMAKQGKTYQKILKYYYPKTMIQKLYE"
     gene            4325..4585
                     /locus_tag="PLC71_04945"
     CDS             4325..4585
                     /locus_tag="PLC71_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRM02021.1"
                     /translation="MKRYDFTITSDRGLHAQPVAALASIACKSASCVTLEYDGGEIDV
                     SNAIRLMSACISCNDKVSLIVSGSDEDETMEKLKEIVTSQLL"
     gene            4682..6136
                     /locus_tag="PLC71_04950"
     CDS             4682..6136
                     /locus_tag="PLC71_04950"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRM02022.1"
                     /translation="MDINHLTLLTDLYELTMMQGYFKTGNDETVVFDVFYRDNPSGSG
                     YAITCGLDQVIDYIKNLSFSYDDIDYLRNQGIFDEDFLEYLAGYHFTGDIYAIAEGTV
                     VFPREPLLKVKAPIMEAQLVETALLNIINHQSLIATKASRVVYAAGGSGVMEFGLRRA
                     QGPDAGTYGARAAVIGGCDGTSNVLAGKCFDIPILGTHAHSWIMSFDDEYHAFRAYAD
                     LYPDSCTLLVDTYDTLRSGVPNAIRVFEELRAEGHMPKHYGIRLDSGDLAYLTKKARI
                     MLDEAGFPDAVIAASGDLDENLITSLKSQGAPITSWGVGTKLITSADCPAFGGVYKLA
                     ASKPKGADEFTAKIKISENPAKITNPGNKTIYRIYGKEDGKIRGDLICLVGEHYDESD
                     DLTIFDPQATWKKSTLAGGTYTMRELLVPIFIKGQCVYKSPSVMDIRSYCKDELNTLW
                     DESRRLVNPQEVYVDLSMPLYKLKNELLDANHKK"
     gene            6163..6897
                     /locus_tag="PLC71_04955"
     CDS             6163..6897
                     /locus_tag="PLC71_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRM02023.1"
                     /translation="MIRFFADIIYLFFFLTLTAPLYPLFDHWNKTGKAHKRSSIAQRM
                     ILRSFKICLAMAGTKLIVDGQENIPEDGAVLYVGNHSSYYDILCSYVATERGMGFFAK
                     KEMEKIPCLSHWMRFINCLFLDRKNIKAGLATMNEGTKLLKDGFSIAIFPEGTRSQDE
                     DPHEFKEGSLRPALKAGVPVIPMAISGTADILENNRRFQVKPTTVHITFGQPIYPDQL
                     ERAEKKHLGATIREEIIEMRKKHKTS"
BASE COUNT         1826 a         1358 c         1237 g         2560 t
ORIGIN      
        1 tgatacgctt gggatattta accattcaga tacttcggaa ccaacctgtg ctgtatcacc
       61 atcggttgtc tgttttccac ataagatcag gtcaaaatct cccatcttct tgattccctg
      121 tgcgatcgta taacttgttg ccagtacatc ggctccacca aatcttcggt ctgataataa
      181 tgctccacgg tcaactccca tggcaaatgc ttctctgatc actgtcattg cctgtggtgg
      241 tcccatactg atgacactga tcgttccacc tacttcttct ctgatcttaa gcgctgtctc
      301 taacgcatac agatcatatg gattcatctt tgaatcggct ccgtttctta acagcacacc
      361 ggttactgga tcaacttcca ctttattaga tgccggtacc tgtttgatac atactaaaat
      421 gtccattgct attcctcctc attcctaaga ctttctatcc tttatcttaa gctcttccgc
      481 tctagattgg tactaccaat tttgtttgaa aaaaatttgg atgtttttcg catccattgt
      541 ttttatctgt ttttgtctta ttggtactac caatttatgt ttttattata tatacattgt
      601 ctacttttgt aaagtactat ttaacatttt tctttttttt gttaatcttg cataaaaaag
      661 aagagccaaa atggctcttt ccttaaagtt ctttgtgaat aatgttatca atcatctcga
      721 aatgtttcat caatgctttc tgtccattga ccacatctct ctttaataaa tattcgacga
      781 tctgcttatg tgcttcctgt aacattcctt ttcgcttctc ggttcttaag atttctcgac
      841 gcatttcaaa aatgaagata tccatcactt cagataatgc atctaagata ttcacgatca
      901 gaacattgcc tgatgctttc gcgatcgcat aatggatctt cttatcgtac attgtcttct
      961 catcgttatc tgtactctga tctaatgcat tcacatattc ttgtaattct atgatgtctt
     1021 ttgtctctgc attcttcact gcaagtgcat atgcctgcat ctccagtccg atacggatct
     1081 ggttgatctg atcataatca agcttatcaa gcataaacat catagaaagg gcatctacca
     1141 tatatccctt aaaatttcct gatatataat ttcctgctcc ttgtctactt gagaccactc
     1201 ccatgacagt taagatcttc aacgcttcac gtacggaatt cctgctgact ccaagcttct
     1261 ctgagatttc tctctctggt ggaaggcctt gtccaagaac aagttcctga ttcatgatct
     1321 gttctttgat actatctatg acgatctcat aggctttctt tgttttttcc atattaatat
     1381 cccattctgt ctattacttt ttttcttaat cacataatag gtaacattat accagattca
     1441 cttttttttg aaaacccctt gcaatatcct ttgaatagtt gtagtatgta agaaagttag
     1501 atgtggtttt taatactacg tattgaattt tataacacta gaatgattgg aggttttacc
     1561 atgaaacata aattaaaaga tccaggaagt gcaattaccc atttgatcgg aatgatcctg
     1621 gccgcgatcg tttccattcc attgatcatc aagagctttt taagcggtga ttatgtacgc
     1681 attatctctt tgatcatatt tactataagt atgatcggtc tttatggtgc aagtactgct
     1741 tatcactctt ttaatatttc accaatgatc aataagaagt taaagaaact tgaccatgca
     1801 atgatctttg ttctgattgc tggttcttac acaccgatct gtacgatatg gacttttatc
     1861 cgtaatcttg atcatagcga tccttggtat cgtatttaag atgttctggg ttacttgtcc
     1921 gaagtgggta tcttctgtca tgtatatcgc aatgggatgg ctttgtatcg ttgcgatcgc
     1981 accgatcatc catagtttat ccaagacatc ttttggatgg ctacttgctg gcggtatcat
     2041 ttatacagtt ggtggtgtga tctatgcact aaagattcct tggttcgaaa atcactggaa
     2101 gaattttggt ctgcatgaga tctttcatct gtttgttatg ggtggaagtt tatgccatat
     2161 gatcctaatg ttccagattt aatttttgct cctgttggta gttttctatt aacaggagtt
     2221 tttcctttct ggtaagacgt ttaatctttg cttctttact cattgcttcc tgctttgtat
     2281 caaattcttc aagatacaca agttctactg gtcctcgtcc tcttgtatat tttgcacctt
     2341 ttccttcatt atgcatcttc aggcgatgtt tgatatcgtt agtccatcct gtgtataaac
     2401 tgttatcact gcattttaat atgtacgtta cattcttcat ctgtcttatc ctctgtctat
     2461 ctgtatattg atccagccct gatcttcatt ttctttgaag aaaggctgtc agatgttctc
     2521 tgaccgcctt tcctctacat caaccagata tgttatggct gttattttct cactttattt
     2581 tattccagat attttaaagg gtctacactt tttttatctt tttccatctg aaaatagaga
     2641 ttggttcctt ctaatgtgta ataacttgtc ggctctgcga cttttgcaat aacttctcct
     2701 gcattgacca ttgttccttc ttttactctg atatctttaa nnnnnnnnnn aagctgtccg
     2761 tataatacgg tatattcatt tccaagatcc atcaataccg tttgtccata ttctgctgat
     2821 tttttaattt ctcttacctt tgccttacgg actgccttca cctcgtctcc ttcttttgct
     2881 tcgatcagga ttcctttgtt gatacggtac tggtcaagag attcaaaata gatggttgaa
     2941 tttacactgt atggaagaat gatattgcct ttgatcggcc atgtcagttt ggatgtacca
     3001 ttatacgtaa cactttcact catggtacgt ttcatttgtg ccgttgtctc tgtttctttc
     3061 ttgtcttgcg ttgttgtttt tgttttttcc tgtgttgcgc tgttattagc tccgatcgat
     3121 tcttcttcca ggctgacttc ctgttttgcc tgatctctcc tctggcggaa tgtcgcactg
     3181 tagtacgcca tgattccaag aaagaatacg aatcctatga tcattgcccc ataatatttg
     3241 cgcagcggaa atgggttttg tttgttattt tttctcattt tcatcacctc aaacatagtt
     3301 ttgcccattt ccatctgttt attcatatag tttttgaatc attgtttttg gatagtaata
     3361 ttttaatatt ttttgatagg tttttccttg ctttgccatc tgatctgcac catactggct
     3421 tagtcctaat ccatttccct ttccgagaca gatgatcctt aaatttcctt tatattgttc
     3481 aaaataaaaa ttattggatg ccagttgcag ccatcttgca aaatcttctc cggaaatgat
     3541 acatgatccg gccttgatct gtttgacata tccattcttt gttaccgtta aaagtctcag
     3601 atttttttgt aattgttgta tggtgatttt ctttttcatg tgttcttcta gtataatctg
     3661 catcctttga tatgaaattg tcttaattga catataatct ttggattcga tatcacttaa
     3721 actttctttc tgtcttagat aaggaatctt tttcccgaac cattcacttg catctaatgt
     3781 catcccaaca ctcactgcat gaaaatatgg ttcgatcaat gctcctttgt atgtgatcac
     3841 ctttccgatt gtttctttga ctgctttttt tcgtttctga tccatgatct cataggcacg
     3901 atcacccagt ttacttttat attgttcgtc tttgtacacc tgatatggta tgctttccat
     3961 ctttatcttc tttgatctgc ccattttccg gatcagatct gtccttaaga tcacagcctg
     4021 gcatttgatc gtttcttctt cttgatccat ggaaatctgt cctgggagga tttttaaaag
     4081 gtaactttcc aaatcaatat cttggtcctt gtcttgtatt ttatatccac tgtcatagtt
     4141 ttcaaacgaa atatttttct tgttttcttt attgatatat atcgtaataa tatatggaat
     4201 cgttgttaac aaaaaaaata aaaataatat atatgtcatt ttgtacctca ttccaatcct
     4261 ccttttgtcc gtcttactat tttatgttac aatagaaaaa aatatgaaag gactcttata
     4321 ttttatgaaa agatatgatt ttacaattac atctgaccgt ggacttcacg cacagcctgt
     4381 agctgctctt gcaagtatcg catgtaaaag tgcttcttgt gttacactag aatatgatgg
     4441 aggagaaatc gatgtatcca atgcgatccg tctaatgtct gcttgtatct cctgcaatga
     4501 taaagtaagt ctgatcgttt ctggatctga cgaggatgaa acaatggaga aattaaagga
     4561 aatcgtcact tcacaacttt tataagaata ctgttcgttt ttgtcagatt atgttatact
     4621 gaagttgtta tagggcagct tatcctgacc cttctatttt tatgttgaaa aggagtattc
     4681 aatggatata aatcatctta ccttgctcac agatctttat gaattaacca tgatgcaagg
     4741 atattttaaa acagggaatg atgaaactgt agtttttgat gttttttacc gtgataatcc
     4801 ttctggaagt ggctatgcga tcacttgcgg actcgatcag gtcattgatt atatcaaaaa
     4861 cttatctttt tcatatgatg atattgatta tcttcgtaat caagggattt ttgatgaaga
     4921 ttttctggaa tatcttgctg gatatcattt tacaggagat atttatgcga tcgcggaagg
     4981 aactgttgta ttcccaagag aaccgcttct gaaagttaaa gcacctatca tggaggcaca
     5041 gcttgtagaa actgcgctcc ttaatatcat caatcatcaa agtctgatcg ctacgaaggc
     5101 ttctcgtgta gtttatgctg ctggtggaag tggtgttatg gaattcggac ttcgacgtgc
     5161 tcagggacct gatgctggta cttatggtgc ccgtgctgcc gtgatcggtg gttgtgatgg
     5221 tacttcgaat gtacttgctg gtaaatgttt tgatattccg atccttggaa ctcatgcaca
     5281 cagctggatc atgagttttg atgacgaata tcatgctttc cgtgcttatg cagatcttta
     5341 tccagattcc tgcactttac ttgttgacac ttatgacact cttcgttctg gtgttccaaa
     5401 tgcgatccgt gtatttgaag aattaagagc agaaggtcat atgccaaagc actatggaat
     5461 ccgtttagac agtggggatc ttgcttatct tacaaagaaa gcccgtatca tgcttgatga
     5521 agcaggtttt cctgatgcag tgatcgctgc ttctggagac cttgatgaga atctgatcac
     5581 aagcttaaaa tctcagggtg caccgatcac ttcatggggt gttggtacaa aactgatcac
     5641 ttctgcagat tgtcctgcat ttggaggtgt ctataaactt gctgcctcta aaccaaaagg
     5701 tgctgatgaa tttactgcta agatcaagat ttctgagaat cctgcaaaga tcacaaatcc
     5761 tggaaataaa acgatttatc gtatctacgg aaaggaagat ggaaagatcc gtggtgatct
     5821 gatctgtctt gtcggggaac attatgatga atctgacgat ctgacaatct ttgatcctca
     5881 ggctacttgg aagaagtcta cacttgctgg tggtacttat acaatgcgtg aacttttagt
     5941 tccaatcttt atcaaaggtc aatgtgttta caagagtcct tctgttatgg acatccgttc
     6001 ctattgtaag gatgaactaa acacattatg ggatgagagc agacgtcttg tgaatccaca
     6061 ggaagtctat gtcgatcttt ccatgcctct ttacaaatta aagaatgaat tgcttgatgc
     6121 aaatcacaaa aaataaaatc atttatagaa gggagaacga tcatgattcg tttttttgca
     6181 gatattattt atctgttctt tttcctgaca ttaaccgcac cgctttatcc attgtttgat
     6241 cattggaaca aaactggaaa agctcataaa cgttcatcca tagcacaacg catgatctta
     6301 cgatctttta agatctgtct tgctatggct ggtacaaaat taattgttga tggtcaggaa
     6361 aacataccag aagatggtgc tgttttatat gttggaaatc atagcagtta ttatgatatt
     6421 ttatgcagct acgttgctac tgaacgtgga atgggatttt ttgccaagaa agaaatggaa
     6481 aagattcctt gtctatccca ctggatgcgt tttatcaact gcctgttttt agatcgtaag
     6541 aatatcaaag ctggacttgc taccatgaat gaaggtacaa aacttttgaa agatggtttt
     6601 tccattgcga tcttcccaga aggtaccaga agtcaggatg aagatcctca tgagttcaaa
     6661 gaaggaagtt tacgtcctgc tctgaaagct ggtgttcctg taattccgat ggcaatttca
     6721 ggtactgctg atatcttaga gaataacaga cgttttcagg taaaaccaac gactgttcat
     6781 attacttttg gacagccaat ctatcctgat cagcttgaaa gagctgagaa aaagcatctc
     6841 ggtgctacaa ttcgtgaaga aattatcgaa atgcgaaaaa aacataagac aagttaagga
     6901 aaaaatgcta taatagttaa gatattcaaa tgtatatatt aggaggagaa gataatatga
     6961 gtgtatggac agttatcctt attattgcgg t
//
LOCUS       DAOMUE010000079         6953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2887_length_6953_cov_2.333140, whole genome shotgun sequence.
ACCESSION   DAOMUE010000079 DAOMUE010000000
VERSION     DAOMUE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2887_length_6953_cov_2.333140"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..688
                     /locus_tag="PLC71_04960"
     CDS             131..688
                     /locus_tag="PLC71_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM02024.1"
                     /translation="MEDQKIIDLYTNREERAIHETDKKYGRLCTSISMGILNDIRDSE
                     ECVSDTWLTLWNQIPPKKPNPFKAYICRIIKNLSLKRYEYHHAKKRNSQYDLSLEELE
                     ECISKEQNVENQIELRELKWIIAKFIEELPKEKRILFLRRYWFLQSVSEMARDYEITP
                     KNMSVKLLRIRKELKEYLKEERFEI"
     gene            685..1179
                     /locus_tag="PLC71_04965"
     CDS             685..1179
                     /locus_tag="PLC71_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02025.1"
                     /translation="MKDNKDEEKTLFQAIDAVDDKFLMETLKDMEKRKNKNHKQQLKF
                     KQMTPMVKAATIALICFLTLGISVSVAAATSKTFRQWIQKTFMSQTIYKVTQTKDVSD
                     HKKNQKIVLKDNMEIKQGHNQSFICQYHYEGKYDVEVTDKVYTIEGNHLRIKTREKMV
                     PWNL"
     gene            1208..1636
                     /locus_tag="PLC71_04970"
     CDS             1208..1636
                     /locus_tag="PLC71_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02026.1"
                     /translation="MIRGEIYVYNVKGNISDIFWKVWDEDTIYVSLYHMNKKDDIIQK
                     ECIAKLNLKTGKVTKITDDTKICNMEMSPDGEWILLNYRSKGYWSALNLKTGKETKQP
                     GISGYAHNEEICFIGKDKIVAMGNPVMTKNSEYNVWNKIE"
     gene            1629..2036
                     /locus_tag="PLC71_04975"
     CDS             1629..2036
                     /locus_tag="PLC71_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02027.1"
                     /translation="MNKINLKTAKATKQWDDRSKEEQYSNNEWYVYKEKKGKLHLKHL
                     AYETSIDIPDVKTVHIIDDAGDYVLFDDDQGNDYLCNLRNKTYKKFILPKKFRDDTQM
                     YLAGKEKKMLVQHGKEIYLIDISDMYKNIQPRK"
     gene            2075..3364
                     /gene="eno"
                     /locus_tag="PLC71_04980"
     CDS             2075..3364
                     /gene="eno"
                     /locus_tag="PLC71_04980"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRM02028.1"
                     /translation="MKNRLCIENVIGREVLDSRGNPTVEVDVLLENGALGRAIVPSGA
                     STGKYEALELRDREKRYRGLGVETAVNHVNHKIADRLTGKNALNQIEIDQIMLEADGT
                     PNKERFGANAILGASLATAKAAACGLGLPLYQYLGGSFASKLPIPMMNILNGGKHADN
                     TVDFQEFMIAPVGADSFKDALMMGTDVYHTLKYILRRNHLNTGVGDEGGFAPNLESSE
                     QALDLLVQAIEQAGYKTKEEIAIAMDCAASEIYNEDEKVYHFMGESRMAGKEVRRNSE
                     EMVQYYEKLVEDYPIFSIEDGLNEEDMPGWKVLTYRLGEKILLVGDDLFVTNVARLRE
                     GIKENIANSILIKPNQIGTLTETLDAIETAAKSNYKTIISHRSGESEDTTIADIAVAT
                     NAGLIKTGAPCRSDRTAKYNRLLRIEEELGSLSKYGW"
     gene            3435..3710
                     /gene="secG"
                     /locus_tag="PLC71_04985"
     CDS             3435..3710
                     /gene="secG"
                     /locus_tag="PLC71_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRM02029.1"
                     /translation="MNHSEWKYIRRHIVKTALTVIFIIASILLIILVLMQEGKEAGLG
                     SLTGAADSGTYWSKNKGRSKEGTLIKVTTFFTIVFFVTAAILCSRFI"
     gene            3814..5971
                     /gene="rnr"
                     /locus_tag="PLC71_04990"
                     /pseudo
     CDS             3814..5971
                     /gene="rnr"
                     /locus_tag="PLC71_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393525.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            5943..6419
                     /gene="smpB"
                     /locus_tag="PLC71_04995"
     CDS             5943..6419
                     /gene="smpB"
                     /locus_tag="PLC71_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRM02030.1"
                     /translation="MSKAKTDGIKLIANNKKARHDYFVEDSFEAGIVLHGTEVKSLRQ
                     GHCSIKESFVDIDNGEVFIHQMHISPYEKGNIFNKDPLRTRKLLLHKSEISKMIGQIN
                     QKGYTLIPLKVYFSGSLVKVQIGLCKGKKLYDKRQDISKRDQLREAQRDFKVRNFG"
     gene            6546..6888
                     /gene="ssrA"
                     /locus_tag="PLC71_05000"
     tmRNA           6546..6888
                     /gene="ssrA"
                     /locus_tag="PLC71_05000"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2622 a         1020 c         1503 g         1808 t
ORIGIN      
        1 ttatttttta tcatatactc aaaatctaca gttggccaac acacaaatta caatttctat
       61 gtagtatcat ataataattc cgtatatata accagatgat catcaaaata aacaaaggaa
      121 gggcaaggat atggaagatc aaaagatcat agatctatat acaaaccgtg aagaaagagc
      181 aattcacgaa acagataaaa aatacggacg attatgtaca agcatttcca tgggaatctt
      241 aaatgatatc agagacagtg aagaatgtgt cagtgataca tggcttacct tgtggaatca
      301 gataccgccc aagaaaccga atccattcaa agcgtatatc tgcaggatta taaaaaatct
      361 atcattaaaa cgctatgaat atcatcatgc aaaaaagaga aacagccagt atgatctttc
      421 actggaagaa ttagaagagt gcatcagcaa agaacaaaat gtagaaaatc agatcgaatt
      481 acgagaatta aaatggatta ttgcaaagtt catagaggaa ctgccaaaag aaaaaagaat
      541 tttattttta agaagatact ggtttttgca atcggtctca gaaatggcga gagattatga
      601 aataactcca aagaatatgt cagtcaaact gttaaggatc agaaaagagt taaaagaata
      661 tctgaaagag gagagatttg aaatatgaaa gacaataagg atgaggaaaa aacattattt
      721 caggcaatcg atgcagtgga tgataaattt ttgatggaaa cattgaaaga tatggaaaag
      781 agaaagaaca aaaaccacaa acagcaatta aaatttaagc aaatgacacc aatggttaaa
      841 gcagcaacga ttgcattgat ctgttttctg accttaggaa taagtgttag cgtggcagca
      901 gcgacatcga aaacatttcg gcagtggatt caaaagacat ttatgtcaca gacgatatat
      961 aaagtaacac aaaccaaaga tgtcagcgat cataagaaaa accaaaagat tgtgctaaaa
     1021 gataacatgg aaatcaaaca aggacacaac caaagcttta tctgtcagta tcattacgaa
     1081 ggaaaatacg atgtcgaagt gacagacaaa gtatatacaa ttgaagggaa tcatttaaga
     1141 attaaaacca gagaaaaaat ggttccatgg aatttataat ggaaggaaat tttcatttca
     1201 gtatgcagtg attcgtggag aaatttatgt atacaatgtg aaaggaaata tcagtgatat
     1261 tttctggaaa gtatgggatg aagatacgat ttatgtttct ttatatcata tgaataaaaa
     1321 agatgatatc atacaaaaag aatgtattgc aaaattaaat cttaaaacag gaaaagtaac
     1381 aaagattaca gatgacacca aaatctgcaa tatggagatg tcaccagatg gggaatggat
     1441 tttgttaaat tacagaagca aaggatattg gagtgcattg aatcttaaaa caggaaaaga
     1501 aaccaaacag ccaggaatca gtggatatgc acacaatgaa gaaatttgtt ttattggaaa
     1561 agacaagatt gtagcaatgg gaaatcctgt catgacaaaa aattctgaat ataatgtctg
     1621 gaacaagatt gaataaaatt aatctaaaaa cagccaaagc gacaaaacaa tgggatgacc
     1681 gctccaaaga agaacaatat tcaaataacg aatggtatgt atacaaagaa aagaaaggaa
     1741 agttacattt aaaacatctg gcatatgaaa catccattga tattccagat gtcaaaacag
     1801 tacatattat tgatgatgct ggagattatg ttttatttga tgatgatcaa ggcaatgatt
     1861 atttgtgtaa tctaagaaat aagacatata agaaatttat attgccaaaa aaatttagag
     1921 atgatacgca aatgtatctg gcaggaaaag agaagaagat gctggtgcaa catggaaaag
     1981 aaatatattt gatcgatatt agtgatatgt ataaaaacat ccaaccaagg aaataatacc
     2041 actattattt tcataaagaa ggatgtgaca aaaattgaaa aatcgtttat gcattgaaaa
     2101 tgttatcgga agggaagttt tagattccag aggaaatcca acggtagaag tcgatgtact
     2161 tttagaaaat ggagccttgg gaagagcgat tgtcccttct ggtgcttcca caggaaaata
     2221 tgaggcgtta gagttaagag accgcgaaaa aagataccgt ggattaggtg ttgaaacagc
     2281 tgttaatcat gtgaatcaca agattgcaga ccgcttgact ggcaaaaatg cactcaatca
     2341 gattgagatc gatcagatca tgttagaagc agatggaacc ccaaacaaag aaagatttgg
     2401 ggcaaacgca atccttggag catcacttgc aactgctaag gcagcagctt gtggactagg
     2461 tttaccgtta taccagtatc tcggaggcag ctttgcatcc aaacttccga taccaatgat
     2521 gaacatatta aatggaggaa aacacgcaga taataccgtt gattttcagg aatttatgat
     2581 tgcaccagtt ggtgcagact ctttcaaaga tgctttgatg atgggaactg atgtgtatca
     2641 tacattaaaa tacattttga ggagaaatca tttaaataca ggtgttggag atgaaggagg
     2701 tttcgccccg aatctggaaa gctcagaaca ggcattagat cttttggtgc aggcaattga
     2761 acaggcagga tataaaacaa aagaagaaat agcaatcgct atggattgtg ctgcatcaga
     2821 aatctataat gaagatgaga aagtttatca ttttatggga gaaagcagga tggcaggcaa
     2881 ggaagtaaga agaaattctg aagaaatggt gcagtattat gaaaagcttg tcgaagatta
     2941 tccaatcttt tccattgaag atggattaaa tgaagaagat atgccaggtt ggaaagtatt
     3001 gacctaccgt cttggagaga agatcctgct agttggagat gatttatttg taacaaatgt
     3061 tgcacggcta agagaaggaa ttaaggaaaa tattgcaaac agcattttga tcaagccaaa
     3121 tcagattgga acattgacag agacattaga tgccattgag acagctgcaa aatcaaacta
     3181 caaaacaatc atttctcata gatcagggga aagcgaagat acgacgatcg cagatattgc
     3241 agttgcaacg aatgcaggac tgatcaaaac aggagcacca tgcagatctg atcgtactgc
     3301 aaaatacaat cgtttattac gtattgaaga agagcttgga agcttatcaa aatatggctg
     3361 gtaaaaggat aaaaatgtaa aaattatgca aagtattgtt gttttccttg caagtttgat
     3421 atgtatatgt tagaatgaac cacagtgaat ggaaatatat taggaggcat atcgtgaaaa
     3481 cagcattaac tgtaattttt attatagcat caattttatt aatcatatta gtattaatgc
     3541 aagaaggaaa agaggcggga ttaggttctt taaccggtgc agcagatagc ggaacatact
     3601 ggagcaagaa caaaggtcgt tctaaggaag gaacactgat caaagtgaca acattcttta
     3661 cgatcgtatt tttcgtaaca gcagcaatcc tttgctcaag atttatttag agaaaagata
     3721 agcacttctt aaacttgttt taagaggtgc tttttatgtc gaagagaatt tccataaaaa
     3781 gattaagaag atttaaatat acaggagaac gtaatgacag ataaacgatt taacagaaag
     3841 aagaaaatta aagcgttgat caacgatccg tattatcagc caatgaaaca aagagagatc
     3901 ggatacctga tgcaggtagc tccagaagat cgtgacgagt ttgttgagat tttgaatgaa
     3961 ctggtagaag aaggatcgat tgaagtaagt aaacgtggaa aatacatgcc gccaagtgtg
     4021 aagacggtca aaggaacatt cagttgtaca agcaaaggtt ttgggtttgt gactgtggaa
     4081 ggggaagatg acgattatta cattcatgag aagaacatga atggagcttt ccatgaagat
     4141 acggtcttaa tgcaggtatt ggacgatcat ccaacttctg gaagaagaaa agaaggaaag
     4201 atcatcaaga ttttagagag aggaatcaag acggtggtcg gaactcttca gaaaaaccag
     4261 aactttgggt tcgtgattcc agataattta agatttgaca gtgatatttt tgtatccaaa
     4321 tctcagtgca agaatcttac cagcggattt aaagtgatgg tagagatcac agattatgga
     4381 gatgccagaa gaagtccaga aggaaagatc attgaagttt taggacataa agatgatcca
     4441 agagttgata ttttatcaat tgtcaaagct tatggaatcc caacccagtt tgaagatgat
     4501 gtcaaacagc aggttgatac gattccatca cagatcaaag catctgacta caaagaccgt
     4561 atggatatca gagactggcc aatggtaacg atcgatggag aagatgcgaa ggacttagat
     4621 gatgcgatca cattatcaag aaaaggaaag aattatttac ttggagtaca tatcgcggat
     4681 gtatccgaat atgtaacaga gtactctcca ttagataaag aagcgttaaa acgaggaaca
     4741 agtgtatatc ttgtagaccg tgtgattccg atgcttccac atcaattatc gaatggaatc
     4801 tgttccttaa atcagggatg cgacagactg gcattaagtt gtatcatgga agttgatcca
     4861 agtggaaagg ttgtcgatca tcagatcgca gagacattga tcaacgtcga tcatcgtatg
     4921 acatatacat ccgtagctaa aatcttggat ggatatggaa gaacgtaaca aatacgaaga
     4981 ttttgtagaa atgtttgagt taatgaagga attatccgac attttaagat atcgtcgtca
     5041 tgaaagagga agcattgact ttgactttcc agaatctaag atcatcttag atgaaaaagg
     5101 ccgtccagtt gatgtttatc cacaggtgag aaatgcagca acaaagatta tagaagattt
     5161 tatgctgtgt gccaatgaaa cgatcgcgga agaattttac tggagagaga ttccattttt
     5221 gtatcgaatc catgagattc cagaccatga gaagattgat aagttacagg tatttttaca
     5281 aaactttgga atttacttaa agagcagtca tgatgagatt catccaaagg aagtacagaa
     5341 gttactgaca aagatcgaag gaacaccaga agaaccattg atcagccgtt tgacattaag
     5401 atctatgaaa caggcaaaat attctgcata cagctctatg cattttggat tgtccacaag
     5461 atattactgc cattttacat caccgatccg aagatatcca gatcttcaga tccacaggat
     5521 catcaaggaa actttgcata ataaatttac aacaagacga ttcagtcatt acgatgcaat
     5581 tttgccaaaa gtagcagaac agtcatctaa gatggaacgc cgtgcagaag aagtcgagag
     5641 agaagtatgt aagcttaaga aagcggaata catgagaaga agaatcggag aagtacatga
     5701 aggaatcatt tccggtgtga caaactgggg aatctatgtg gaacttccaa atacgatcga
     5761 aggaatggtt catgtatcca ttcttccggg agactattat tactatgatg agaagaccta
     5821 ttccatggta ggagaaagaa ctggaagaac atttaaatta ggagagaagg cgaaaatccg
     5881 tgtcaaagat gtggatatga tgcttaagaa tattgatttt gagttagtgg aggatgatga
     5941 ttatgagcaa agcgaagacg gatggaatta aactcatagc aaataataaa aaagcaagac
     6001 atgattattt tgtagaagac agttttgaag cagggatcgt acttcatgga actgaagtaa
     6061 aatctttaag acaaggacac tgcagcatca aagaatcctt tgttgatatt gataatgggg
     6121 aagtgttcat tcatcagatg catatcagcc cttatgaaaa aggaaatatc ttcaacaaag
     6181 atccacttcg tacaagaaaa ctattattgc ataaatcgga gatttccaag atgatcggac
     6241 agatcaatca gaagggatat acattgattc cattaaaagt atactttagt ggaagtttgg
     6301 tgaaagttca gatcggatta tgcaaaggta agaaactcta cgacaaacga caagatatct
     6361 caaagagaga tcagttaaga gaagcacaga gagacttcaa agtaagaaac tttggataat
     6421 ttcaaggaag attaaagatt gtataagaag ttatacaaaa agacttgaca tcctatataa
     6481 gagactatat aatataattt cgagactggg aaggctctca gtctcgaacc aagcttatta
     6541 attccgggct agtaaaggtt tcgacagggg ttatgaagct tgaggagcca ttgggaggga
     6601 ccaccttacg gtacaaactt aaatataaac gcagataata aattagcgta cgctgcctag
     6661 tttgcagcag tcagctctag atcacctgta gtctggaatc ctggcttcaa acatgcagga
     6721 cccttgaccg gtcaagcttt gtgccggtta aagtattgat gaagctactg aagctgtgat
     6781 cctgttagtc ggagcacggt agagggaatg aaagaagact gactgtaatg ggagagcctc
     6841 atgtgaattg gcttttggac gcgggttcga ttcccgccta gtccattctt aaacctcacg
     6901 tttttacgtg gggttttctt ttttcatgtt gcattcgtgt tgcaatatat ttt
//
LOCUS       DAOMUE010000080         6834 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2960_length_6834_cov_3.367163, whole genome shotgun sequence.
ACCESSION   DAOMUE010000080 DAOMUE010000000
VERSION     DAOMUE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2960_length_6834_cov_3.367163"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLC71_05005"
     CDS             complement(<1..511)
                     /locus_tag="PLC71_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRM02031.1"
                     /translation="MLAQERFERILEILREKQSVTVVELTEKLNTSESTIRRDLTELN
                     RQGLLIKVHGGATAIKSVLSREEEVITKSKKHQEEKRKIAKYAASLINKEDIVYLDAG
                     TTTEMMLEYLKEYDVVYVTNGITHARKLMNAGFKVHLIGGEIKAVTEALVGEEALEQL
                     DKYNFTKGFF"
     gene            complement(769..2817)
                     /locus_tag="PLC71_05010"
     CDS             complement(769..2817)
                     /locus_tag="PLC71_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM02032.1"
                     /translation="MYSKIAFSNVKKSIRDYTIYFLTLTFGICLFYMFNSVQAQQAVL
                     KLNASQKSMMHLMSVIINGISVFVAIILGFLIVYANQFLMKRRHKEMGIYLLLGMEKR
                     QVSKILLIETLLIGVLALIAGLVSGVFLSQGLAMLTAKMFAVQMKEFKFVFSQMAFIQ
                     TILYFAIIFIIVMIFNGITISKYKLINLLNSAKKNQKTRLKNPILSVILFLISIGILG
                     IAYHLIQKNQMLAVDNDFKISVLLGVAGTFLFFFSLSGFLLELAKRSKKFYYNGLNMF
                     VLRQINSKITTTFVSMSMLCLMLFLAISAFATGSGLASSVKTDLEDMTKFDCTFYGLS
                     EKGYQEEQQQKFIKKLDDLGLTIKKDAKEILPITIYQNGTFRKCRYKMEPLLKGREKY
                     SDYKKDYVKKLYETPLTFAKLSEYNKIRKAIGEKELTLKSDEYILNCDYGNLIPIMEK
                     AASDKQKLTLDGKTYKMKYGLQKDTYMVTAAKTDMGTVILNDEIIQSLKIDHSTLYLN
                     LNWKGNAQKVSRKYTEYLKQMIINSKMPNSLYSATISKEEVYEQSTGLSTIMTYIAIY
                     IGLVFLITCAAVLALQQLSENADNIEKYKLLSKIGTSQKMINRAIKAQIILYFCLPLS
                     LALIHSYVGIKTAKILISTLGQINITKAAAQSLILVIVIYIGYMIATYLGSKSMLKEK
                     "
     gene            complement(2807..3574)
                     /locus_tag="PLC71_05015"
     CDS             complement(2807..3574)
                     /locus_tag="PLC71_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM02033.1"
                     /translation="MSSVLKVDNIEKYYGSKANLTKAIDRISFEVEEGEYIGIMGASG
                     SGKTTLLNCISTIDQVTAGNIMINGMDITKLKGKHLSRFRREELGFIFQDFNLLDTLT
                     AFENIALALTIMKIPAREIEDRVRSIAEKLMITEVLNKYPYQMSGGQKQRVASARAMI
                     TNPSLILADEPTGALDSKAARMLLDSMETMNVELNATILMVTHDAFTASYCKRILFIK
                     DGKIFNELVRGNDSRKRFFDRIIEVVTLLGGDSTNVF"
     gene            3752..4102
                     /locus_tag="PLC71_05020"
     CDS             3752..4102
                     /locus_tag="PLC71_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRM02034.1"
                     /translation="MFLKNEDVKIGTIPDANGSDNPLKKMEFLTEKEMKDSSRLFGKV
                     IVPPGSALAYHTHEGEGEAYHILQGKGLYNDNGTKYEVHPGDTTFTCDGAGHAIENIG
                     DEDLVFMALILLDK"
     gene            complement(4181..4966)
                     /locus_tag="PLC71_05025"
     CDS             complement(4181..4966)
                     /locus_tag="PLC71_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRM02035.1"
                     /translation="MFADYHVHSRFSEDTDFPMELEIQKAIKLGMDELCFTEHSDYDV
                     PTVVNCDYDAYFEEMEKMKDKYKGQITLKTGIEFGIQTHTTKAYEETFRQYPFDFVIF
                     SCHQVENKEYWRQEPQEGKTQLEYNRRYYQEILDVVKVYKDYSILGHLDVIKRYDKQG
                     EIEFEKIQDLIEQIFEVVIADGKGIELNTSSRAYKLKSLMPSKEILKLYHDMGGKIIT
                     IGSDAHNADRIGDCVMESQKILKDIGFDGIYTFEKMKPEFHRF"
     gene            complement(4985..6001)
                     /locus_tag="PLC71_05030"
     CDS             complement(4985..6001)
                     /locus_tag="PLC71_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM02036.1"
                     /translation="MKTKDFFRMEYAQILIYIVFLGLLEVLLFIFEVNYFLMIYLLLI
                     GLILEGVYLCQRYMKKYRYYKKVKAKLDILEQKCMITEMLELPDFAEGEFLYEILQTC
                     NKSMNDEILKYKIEAAGYREYIEMWIHEVKTPLAATRLILENHPSEITRAMDEEMQQI
                     NYYLEQALYYARSNSVEKDYLVKELSLKEVVENAVKANAKSLIGNKIKIKIENLDQKI
                     FSDEKWVLFILNQVISNAIKYKKEEAEISFCARCEKNEILLEIRDNGIGISPKDLPRI
                     TDKGYTGTTGRNYQKSTGMGLYLCKKLCSKLQLSFYIESKEEEYTKVTIGFPRNSMIF
                     LNEE"
     gene            complement(5998..6666)
                     /locus_tag="PLC71_05035"
     CDS             complement(5998..6666)
                     /locus_tag="PLC71_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM02037.1"
                     /translation="MKAKIIIVEDHEPIRNELKILLSKYGYEIIVLDRFDTVVEDCLR
                     EDVDLILLDINLPFYDGYYVCRAIREKSEVPIIVVTSRDSEMDELMSINLGADDFITK
                     PYNTQILLARISAMLKRTHKEKVNDFLQYQGLKLQLNNSTVIYKDQIIELSKNEMRIL
                     YTLLQHKESIVSRDQLMESLWQSNEFIDDNTLTVNINRVRKKLEAAGLKDFIKTKRGQ
                     GYMI"
BASE COUNT         2035 a         1406 c          864 g         2529 t
ORIGIN      
        1 cgaaaaagcc cttagtaaag ttatatttat caagctgttc taatgcttct tctccgacca
       61 atgcttctgt taccgcctta atctctcctc cgatcaaatg gaccttaaat cctgcattca
      121 ttagttttct cgcatgtgtg atcccatttg tcacataaac aacgtcatat tctttgagat
      181 actctaacat catctctgtc gttgttcctg catcaagata cacgatatct tctttattga
      241 tcaaagatgc tgcatatttt gcaatctttc gcttctcttc ctggtgtttc tttgacttgg
      301 ttatgacttc ttcttctctt gataacacag acttgatcgc agtagctcct ccatgaacct
      361 tgatcaaaag accttgtcgg tttaattccg ttaaatccct tcgtatcgtg gactctgagg
      421 tgttcagttt ttctgtcagt tctaccacgg tgactgactg tttctctctt aaaatctcta
      481 aaatgcgttc aaatcgctct tgcgctaaca caatatcacc tcttttcgct tttatttaag
      541 tatatcacaa tttcaatcat agtcaatcat tttctttcat tttcaatcat gtatattctt
      601 catagtttgt tcaggttttc aaaataattt gaacacattt gatttttata ctaatgaaca
      661 taaggaggta ctgtaaaatt aaatgcttcc aaacagacat taaccggtaa cattattaat
      721 agctcaaaaa aagaaatgac agatttggat cttctgtcat ttctttctct atttttcttt
      781 cagcatactc ttgcttccta gataagttgc gatcatatat ccaatataaa ttacgatcac
      841 aaggatcaat gactgagctg ctgcttttgt tatattaatt tgtcctaacg ttgatatcag
      901 aatttttgct gttttaattc ctacataaga atggatcaat gccaaactta aaggcagaca
      961 aaaatacaat atgatctgtg ctttgatcgc acgattgatc attttttgtg aagttccaat
     1021 tttactcaat aatttgtatt tttcaatatt atctgcattc tcacttaatt gctgtaatgc
     1081 aagtactgct gcacatgtaa ttaaaaatac cagtccaata taaattgcaa tatatgtcat
     1141 gatcgtactt aacccagtac tctgttcata tacttcctct tttgaaattg tcgctgaata
     1201 tagtgaattt ggcatcttgc tatttatgat catctgcttt aaatattccg tatattttct
     1261 tgatactttc tgtgcattcc ccttccagtt cagatttaaa tataatgtag aatgatcaat
     1321 ctttaatgac tggataattt catcatttaa aatgactgtt cccatatctg ttttagctgc
     1381 tgttaccata tatgtatctt tttgtaagcc atatttcatt ttatatgttt ttccatccaa
     1441 tgtaagtttt tgcttatcac tagctgcctt ttccattatc ggtatcagat tgccataatc
     1501 acaattcaat atatattcat ctgatttcaa ggttaattct ttttctccga ttgcttttcg
     1561 gattttatta tattcagata gttttgcaaa agttagtgga gtctcataaa gtttcttcac
     1621 ataatctttt ttataatctg aatacttttc tcttcccttt aataatggct ccattttata
     1681 tctgcatttg cgaaatgtac cattctgata tattgtgatc ggtaaaattt cttttgcatc
     1741 ttttttgatc gttaatccaa gatcatctaa tttctttata aacttttgct gctgttcttc
     1801 ctgatatcct ttttccgata atccataaaa agtacaatca aactttgtca tatcttctaa
     1861 atctgttttc actgaagacg ccaatccact tcctgtcgca aacgcactga ttgctaaaaa
     1921 cagcatcaaa cacagcattg acatagaaac aaatgttgtg gtgatctttg aattgatctg
     1981 tcttagcaca aacatattta atccattata ataaaatttc ttacttcttt ttgccaattc
     2041 taataagaat cctgataacg aaaagaaaaa gagaaatgtt cctgctacac caagtaatac
     2101 gctaatctta aaatcattgt ccacagcaag catctggttt ttttgaatta aatgatatgc
     2161 tattccaaga atcccaatgg atatcaaaaa cagaatgacc gacaatatag gattttttaa
     2221 tcttgttttc tgatttttct ttgctgaatt caacagattg atcagtttat attttgatat
     2281 cgtaattcca ttaaatatca tcacaataat aaaaataatt gcaaaataca aaatagtctg
     2341 aataaatgcc atttgcgaga aaacaaactt aaactctttc atctgaaccg caaacatctt
     2401 tgctgtcaac atcgcaagtc cttgtgataa aaacacacct gaaaccaatc ctgcgatcaa
     2461 tgccaaaaca ccgatcaata aagtctctat cagaagaatc tttgatacct gtcgtttttc
     2521 cattcctaac aacagataga ttcccatttc cttatgccgc cgcttcatta gaaactgatt
     2581 tgcataaaca atcagaaatc caagaataat tgcaacaaac acagaaattc cattaatgat
     2641 cacagacatt agatgcatca ttgatttctg gcttgcattc agctttaaga cagcctgttg
     2701 tgcctgaaca gaattaaaca tataaaacaa acaaattcca aatgttaatg tcagaaaata
     2761 aatcgtataa tcacggatac tctttttgac attactaaat gcgatcttag aatacatttg
     2821 tactgtcacc tccaagcaat gtcacaacct caatgatacg gtcaaaaaat cgttttctcg
     2881 aatcatttcc tcgaaccaat tcattaaaaa tctttccatc cttgataaac aaaatacgtt
     2941 tacagtaact ggctgtaaat gcatcatgtg ttaccatcag aatcgttgca tttaattcca
     3001 cattcatcgt ttccatactg tcaagcagca ttctggccgc cttgctgtcc aaagctcctg
     3061 ttggctcatc cgctaagatc agagatggat ttgtaatcat cgctcgggca cttgccactc
     3121 tctgcttctg tcctcctgac atttgatatg gatatttgtt caatacttct gtgatcatca
     3181 acttttctgc gatcgatctg actctgtctt caatttctct tgcaggaatt ttcatgatcg
     3241 taagcgccaa cgcaatgttt tcaaatgctg tcaatgtatc taacagatta aagtcttgga
     3301 agataaatcc taattcctct cgtctgaatc ttgaaagatg ttttcctttc aattttgtga
     3361 tatccattcc attgatcata atatttcccg cagtcacctg atcgatcgtt gaaatacaat
     3421 ttaataatgt cgtcttccca cttccggaag cccccataat tccaatatac tccccctctt
     3481 ccacttcaaa actgatacga tcgatcgctt ttgtaagatt cgctttactc ccataatatt
     3541 tttctatatt atctactttc aatacagaac tcatttttcc atctccttat cttttttggt
     3601 ttctcttatt gtcttgtcta taatatacca gtaaatcatc ttcgtatcca tcgaacttcc
     3661 ttacacaaaa cttacaattt tgtcatattg ctattttatc aaaaagctac tatactaaac
     3721 ttaacatgac tatataaagg agggactcaa catgttttta aaaaatgaag atgtaaaaat
     3781 tggcacaatt cctgatgcaa atggttctga taacccatta aagaaaatgg aattcttaac
     3841 agaaaaagaa atgaaagata gcagtcgtct gttcggaaaa gtcatcgtac cacctggttc
     3901 tgctcttgca tatcacactc atgaaggtga aggcgaagct taccatatcc ttcagggaaa
     3961 aggactttat aatgacaatg gtacaaaata tgaagtacat ccaggagata ctacattcac
     4021 atgtgatgga gctggacatg ccattgagaa tatcggagac gaagatcttg tatttatggc
     4081 actaatctta cttgataaat aatcttgaaa tctaaaatat agcacaatat gtaaagaggt
     4141 gtctatttgt taaaaacaat aggcacctct ttaatttatc ttaaaatcta tgaaattctg
     4201 gtttcatctt ttcaaaagta taaattccat caaaaccaat atcttttaaa atcttctgtg
     4261 actccattac gcagtcaccg atccggtctg cattatgtgc atctgatcca atcgtaatga
     4321 tcttacctcc catatcatga tacagtttca gaatttcttt tgatggcatt agacttttca
     4381 gtttatatgc acggcttgaa gtattaagtt caattccttt tccgtctgcg atcacaactt
     4441 caaaaatctg ctcgatcaga tcctgaatct tttcaaattc gatctctcct tgcttatcat
     4501 atcttttgat cacatcaaga tgaccaagaa tgctgtaatc tttgtatact ttaacaacat
     4561 ccaaaatctc ctgataatac cttcgattgt attctaactg tgtctttcct tcctgtggct
     4621 cttgtctcca atattctttg ttttcaactt gatgacaaga aaagattaca aaatcaaacg
     4681 gatactgtct gaatgtttct tcatacgctt tcgtcgtatg cgtctgaatt ccaaattcaa
     4741 ttcctgtctt taatgtgatc tgccctttat acttatcttt cattttttcc atttcttcaa
     4801 aataagcatc ataatcacaa ttaacgacag ttggcacatc ataatctgaa tgttctgtaa
     4861 aacataattc atccatccct aatttgatcg ctttctgaat ttcaagttcc attgggaaat
     4921 ctgtgtcttc actaaaacgt gaatgtacat gataatctgc aaacattgta aacctcctgt
     4981 ctgatcattc ttcatttaaa aaaatcatag aatttcttgg gaagccgatt gttactttcg
     5041 tgtattcttc ttctttagat tcgatgtaaa aacttagttg taatttgctg caaagtttct
     5101 tacacaaata taaccccatc cctgttgatt tttgatagtt tcttcctgta gttccagtat
     5161 accctttgtc cgtgatcctt ggcaaatctt ttggtgagat tccaattcca ttgtctctga
     5221 tttccagaag tatttcattc ttttcacatc ttgcacaaaa tgatatttct gcttcttctt
     5281 ttttatattt tattgcattg ctgataactt gatttaatat aaataaaacc catttttcat
     5341 cgctgaagat cttctgatcc agattctcta ttttaatttt tatcttattt ccgatcagac
     5401 tttttgcatt tgcttttacc gcattttcta ccacttcttt taaacttaac tctttcacta
     5461 gatagtcttt ctctacacta ttactcctcg cataatacaa tgcctgctcc aaataataat
     5521 tgatctgctg catttcttca tccattgccc gtgtgatctc acttggatga ttttcaagga
     5581 tcagtcttgt tgctgccaat ggtgttttta cttcatgaat ccacatctca atatattctc
     5641 ggtatcctgc tgcctctatc ttatatttca atatctcatc attcatggac ttgttgcacg
     5701 tctgcaatat ttcatataga aactctcctt ctgcaaaatc tggcaattct aacatttctg
     5761 ttatcatgca tttttgttcc aatatatcca gttttgcttt cactttttta taataccgat
     5821 attttttcat ataccgctgg cataaataaa ctccttccag aatcaatccg atcagcagca
     5881 aatatatcat aagaaaataa ttcacttcaa aaataaaaag caacacttcc aataatccca
     5941 aaaatacaat atatatcaga atctgggcat actccattct aaaaaaatct ttggttttca
     6001 tatcatataa ccctgccctc tttttgtttt tataaaatct ttcagtcctg ctgcttctaa
     6061 tttttttctc actcgattta tattcactgt taatgtatta tcatcaataa attcatttga
     6121 ctgccataag gattccatta attgatccct gcttacaata ctctctttat gttgcagtaa
     6181 tgtatacaat attctcatct catttttact aagttcgatt atctgatctt tataaataac
     6241 agtactgtta ttgagttgta gtttcaagcc ttgatattgt aaaaaatcat ttactttttc
     6301 tttgtgtgtt cgttttaaca ttgcagaaat tcttgccaac aaaatctgtg tattgtatgg
     6361 tttcgtgata aaatcatccg ctccaagatt tatactcatt agctcatcca tctcgctatc
     6421 cctacttgta acaacaatga tcggtacttc tgatttttct ctgattgctc tgcacacata
     6481 ataaccatca taaaacggaa gattaatatc taataaaatc agatctacat cttctctcaa
     6541 gcaatcttca acaactgtat caaaacgatc taacactatg atctcatatc catatttact
     6601 taacaaaatc tttaattcat tacgaattgg ttcatgatct tccacgataa tgatctttgc
     6661 tttcatttgc ttccttatca cctttcctta aacctttcct tatcgtatat aatatcataa
     6721 acctttataa aagaaaaagc gcctcacatt ttgagacgct ctttttgaat ttctatttct
     6781 tattttttag gctgcagaga ttcttttcca cccatatatg gtcttaaaac ttct
//
LOCUS       DAOMUE010000081         6831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_2963_length_6831_cov_2.910862, whole genome shotgun sequence.
ACCESSION   DAOMUE010000081 DAOMUE010000000
VERSION     DAOMUE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2963_length_6831_cov_2.910862"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(341..1066)
                     /locus_tag="PLC71_05040"
     CDS             complement(341..1066)
                     /locus_tag="PLC71_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM02038.1"
                     /translation="MKTVLITGSSRGIGRETAIYFAQQGWNVIIHGFRHPEKLESLKN
                     EILEENVSCLSFCGDISDPSFVDEMIKQSLLTFSKIDCLVNNAGISLVGLFTDATVDD
                     WNLMINTNLTSVFTTCKNIVPHMLHHHEGKIINISSIWGDAGASCEVLYSTTKGGINA
                     FTKALAKEVAASNIQVNAIAFGMIDTEMNAEFDEEDLALIKEEIPADYIASPKEAAQM
                     VYHVATSPAYMTGQVITFSGGWY"
     gene            1255..1698
                     /locus_tag="PLC71_05045"
     CDS             1255..1698
                     /locus_tag="PLC71_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02039.1"
                     /translation="MAEKKKSKLGAKAIFARILAVILVTAIGGVASFFGFKTYFSNKL
                     KKDKKAEIAKQLKEESKERVDVGMIQTGNSIVIRIYHNKNQEMIFVPLRQDMNLTLTK
                     KGKQAVEQTLGTSVSKATVADVIKATRKNGSRLRRHWEFLLIPMN"
     gene            1698..2504
                     /locus_tag="PLC71_05050"
     CDS             1698..2504
                     /locus_tag="PLC71_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HRM02040.1"
                     /translation="MSHKKFVKLMNQAGDVKIEFDEAMAYTDSTDKYVTLSAGENSLN
                     GTAIYSLLSESDIFTDKNKQAEITGEICVAVASALNDKTLSEYREYAQNYFDAVKTDA
                     SYEEAATSLERMHGIKDKNLNFKVLDGTESNGKFELDTEEAKRVFDEMLSEEGDLSSA
                     LSTTEAKSTTTKSDSSASSSKNITIEIQNSTRISGLAGRWKDKLASDGYSVGSVRTNR
                     QGVLTHTKIIVESKDLGQDLKSYFKNPEYEVGSVDSGARICIIVGSEDEI"
     gene            2760..3845
                     /locus_tag="PLC71_05055"
     CDS             2760..3845
                     /locus_tag="PLC71_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM02041.1"
                     /translation="MISNQILQETINGMKEVTGIDLCILDLSGRIVVTTMEITDALAQ
                     HAEEFADSMAESQVIDGFQYFKIFDDQRLEYILLATGMSENAYTVGKLAVMQIQNLLT
                     AYKERLDKENFVKNLLRDNLLLVDIYNRSKKLHIEVEQRRVAFLIQAEYEKDQNLLEA
                     TKEVESGADSDFITAVDEKSIVYVTPVNGNEGQAQFIQHAKEIKEGLNEYGIKDAKIA
                     IGNVAETIKDVSRSYKEAQIALEVSKIFYEDASVIAYNQLGIGRLIYQLPLPLCKMFI
                     REVFGDYSPDSIDEETLATIDKFFENNLNVSETSRQLFIHRNTLVYRLDKIQKNTNLD
                     LRNFEDAIAFKIALMVVKYMKHMDTMD"
     assembly_gap    3858..3867
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3868..4521
                     /gene="ftsE"
                     /locus_tag="PLC71_05060"
     CDS             <3868..4521
                     /gene="ftsE"
                     /locus_tag="PLC71_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRM02042.1"
                     /translation="EYNKGNIGLEDVSLDIAKGEFVFIVGKSGSGKTTLMKLLQKELD
                     VTSGQIIVNGQDYKKLTQRNLPKFRRQMGMVFQDFRLLKDRTIFENVAFAQQVVEVPH
                     RYIKHQVENMLEMVGLKDRMNAFPNELSGGEQQRVAIARALVNHPVLLLADEPTGNLD
                     PVTAKGIMELLSEINKSGTTVLVVTHNKELVNEMGKRVILIEDGHIEQDEIRGSYDI"
     gene            4511..5419
                     /gene="ftsX"
                     /locus_tag="PLC71_05065"
     CDS             4511..5419
                     /gene="ftsX"
                     /locus_tag="PLC71_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRM02043.1"
                     /translation="MIFRRFLYGIKEGTRNIFRNKMFSLASLGTIIACVFLLGTFLSV
                     ALNVRSTMQQMEQSVGISVFFEPGISAERKDQIGEEIKKKEDIATMRYVSAEEAWESY
                     KKDVFQGNEELLEGFEGNNPLKESDSYEITLKEVKSYKQVVSHLETIDGVRKVRYSEG
                     AAEGMMKINTMGTYLAGGIILILMAVSIFLVSNTVSIGVAVREEEISIMKLIGAANGF
                     IKAPFQAEGMIIGILGSLIPTAIIFAGYEGIINWMNDKFHVVTVFMKFVPIQQIMLYL
                     LPLALLVGIVIGLLGSSITIRKYLKV"
     gene            5432..6661
                     /locus_tag="PLC71_05070"
     CDS             5432..6661
                     /locus_tag="PLC71_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRM02044.1"
                     /translation="MKRRYQAGIIMMLISAMIASNLPMTTVFAAAKKQQVKQETKKLE
                     EQSRKMQQEIKDLDEKMIKSNDAYEACQEKLISVQKQLKKTQQELKEAKASKEDQSRI
                     MSKRIKFLYENGNMAYMEVIFEANNFQEFLKRADYVSKISKYDSNMFLQLQTTEDKIR
                     MATKSLKQDYQNTKTLTAKAKTEKEKLDQAAAKKKSKLASYQKQLASDKELLAWFEAE
                     EKRQEEMDLASAKDGNADNTTSKAQSEKNMTGSTASKNTTSKATTESKKETTTTTSPS
                     TTVSSGNLSWPVPSCHSISSGYGYRIHPVTGVRKLHAGIDIPCSTGTTIVAAASGTVV
                     DAGYNAYNGNYLKISHGNGLETMYLHCSKLLVSSGARVSGGQMIAKSGATGMVSGAHL
                     HFVVKKNGNYVNPQNYL"
BASE COUNT         2408 a         1046 c         1559 g         1808 t
ORIGIN      
        1 acggaagatg aaagggctta aacaagcacc gatttgttgg gatgtttagg agtataataa
       61 acacttatct tctgtcttga aaaaactaat ttacatccaa gatcattctc catacacatt
      121 cggtagtggt ttcgattacg taaattattt gaatattata aaaagggtta tctgtctgag
      181 accgtaggaa cacgcctgca cttagtgagt ggatggtcgt tagaccattc aggagcttag
      241 tacagctgcg tgtgagtcga gcgagagcgc gtcgcagaca gataaccctt tttataatat
      301 tccaccaata atttccgtca tcaaaccagc aaattaaaat ttaataccat cccccagaga
      361 atgtaataac ctgtcctgtc atataagcag gggacgttgc cacatgataa accatctgtg
      421 ccgcttcttt tggactggca atatagtccg cagggatttc ttctttgatc aaggcaagat
      481 cttcttcatc aaactccgca ttcatctccg tatcgatcat cccaaaagcg atcgcattta
      541 cctgaatatt gcttgcagca acctcttttg ccagagcctt tgtaaaagca ttgataccac
      601 cttttgttgt agaatataaa acctcacagg aagcgccggc atctccccag atggaagaaa
      661 tattgatgat ctttccctca tggtgatgaa gcatatgggg aacgatattt ttacaggttg
      721 taaaaacaga agttaaattt gtgttgatca tcagattcca atcatctacc gttgcatctg
      781 taaataatcc caccagagag attccggcat tatttacaag acagtcgatc ttagaaaaag
      841 taaggaggga ctgtttgatc atttcgtcta caaaagaagg atcactgata tctccgcaaa
      901 aagataagca agacacgttc tcttcaagaa tttcattttt tagggattct agtttttcag
      961 gatggcggaa gccatggatg atcacgttcc atccttgttg tgcaaaatat atggcagttt
     1021 cacgtccgat tcctctggaa ctgccagtta tgagtactgt tttcatagaa aatacctcgt
     1081 aaatcttata aaatttctta gcacttgata tgatcaatct gatcaacgat gagaaatgtt
     1141 gcagattaag aaaatattaa ggcgattata cagaattata acatagagga ttgttgaaat
     1201 aaatcgaata atttgttata ctaaacaaag tttataaacg gaaggagcag gaacatggca
     1261 gagaagaaga aaagtaaatt aggtgccaaa gccatatttg ccaggattct tgcagtgata
     1321 ctggtgacag ccatcggtgg agttgcaagc ttttttggat ttaagacata tttttcaaat
     1381 aaattaaaga aagacaagaa agcagaaatc gcaaagcagt taaaagaaga aagcaaagaa
     1441 agagtagacg ttggaatgat ccagacagga aattccattg tgattcgaat atatcataac
     1501 aagaatcagg agatgatttt tgtaccactt agacaagata tgaatcttac attgacgaag
     1561 aagggaaaac aggcagtcga acagacactg ggaactagcg ttagcaaagc gacggttgca
     1621 gatgtgatca aagcaacaag aaagaatgga agcaggttga gaagacactg ggaatttcta
     1681 ttaattccta tgaactgata tcacataaga agtttgtgaa actgatgaat caggcaggag
     1741 atgtcaaaat agaatttgat gaagcaatgg cttatacaga ttctacagat aaatatgtaa
     1801 cattaagtgc tggggagaac agcctgaatg gaacagcaat ctattcatta ttaagtgaga
     1861 gtgatatttt tacagataag aacaagcagg cagaaattac tggagagatc tgcgtggcag
     1921 ttgcatctgc attgaatgat aagacattat ccgaatatcg tgaatatgca cagaattatt
     1981 ttgatgcggt gaagacagat gcttcttatg aagaagcagc gacaagtctt gagagaatgc
     2041 atggaattaa ggataagaac ttaaatttca aagtgttaga tggaacagaa tccaatggaa
     2101 agtttgaatt agatacagaa gaagccaaga gagtctttga tgagatgtta tcagaagaag
     2161 gagacttaag ttcggcattg agcacaacag aagcaaagtc aacaacaacc aaatctgata
     2221 gttcagcatc ttcttcaaag aatataacga tcgagattca gaattcaaca agaatttcag
     2281 gacttgcagg aagatggaaa gataaactgg caagtgacgg atacagtgta ggatctgtaa
     2341 gaacaaacag acagggtgtt ttaacacata caaagatcat agtagaaagt aaagatttag
     2401 gacaggatct gaaaagttat tttaagaatc cggaatatga agttggaagt gttgacagcg
     2461 gtgcaagaat ttgtataatt gttggatcag aagacgagat ttaaaataca ttcataaata
     2521 ttattataaa atatcacgaa aaacatctca ggaacgattg agatgttttt ctttgttata
     2581 tgaaggggtt acggctaaaa agagatattt atggtcaatg aaataaagat tttctgctag
     2641 atatttgtat aaaatagaca atgatataaa aaaatcacta aatttttctt ttttttttgt
     2701 ttaaaatgct gtatagtaaa agaaaaggtt gaacggcaaa tatgaaaggt tgataaagga
     2761 tgatttcaaa tcagatatta caagagacaa ttaatggaat gaaggaagta accgggatcg
     2821 atctgtgtat tttagatctg tcaggacgta tagttgtaac aacaatggag ataaccgatg
     2881 cattagcaca acatgcggaa gaatttgcag attctatggc agagagccag gtgatcgatg
     2941 gttttcaata ttttaagatt tttgatgatc agagactgga atatatactt ctggctactg
     3001 gaatgagcga gaatgcatat acggtcggaa agcttgcagt gatgcagata cagaatcttt
     3061 tgactgcata taaggagaga ctggataaag agaactttgt aaagaacctg ttgagagata
     3121 acttattatt ggttgacatt tataaccgct caaagaagct gcatattgaa gtggaacaga
     3181 gaagagttgc atttttgatt caggcagaat acgagaagga tcagaatctg ttagaagcta
     3241 cgaaagaggt tgaaagcgga gcagattcag attttattac ggcagtggac gagaagagta
     3301 tcgtttatgt aacaccagtg aatgggaatg aaggacaggc acagtttatt cagcatgcaa
     3361 aagagattaa agaagggctg aatgaatatg ggatcaaaga tgccaagatt gcgatcggta
     3421 atgttgcaga gacgatcaaa gatgtatcaa gatcttataa agaggcacag atcgcattgg
     3481 aagtcagcaa gatcttctat gaggatgcga gtgtgatcgc atacaatcag ttaggaattg
     3541 gacgactgat ctatcaactt ccacttccat tatgtaagat gtttatccgt gaagtgtttg
     3601 gagattattc tccagactcc attgatgaag aaacattggc aacgattgat aaattctttg
     3661 aaaataatct gaatgtgtca gaaacatcca gacagttgtt tattcataga aatacactcg
     3721 tataccgtct ggataagatt cagaagaata cgaatctgga tctgaggaat tttgaagatg
     3781 caatcgcatt taaaattgca ctgatggtag taaaatatat gaaacatatg gatacaatgg
     3841 actagaatat gatgatcnnn nnnnnnngaa tataataaag ggaatatagg acttgaggat
     3901 gtctcattag acattgcgaa aggcgaattt gttttcatcg taggtaagag cggatcaggg
     3961 aagacaaccc tgatgaagct tttacagaaa gaacttgatg tgacaagtgg acagatcata
     4021 gtaaacggtc aggattataa gaaactgaca cagaggaatc tgccgaagtt ccgcaggcag
     4081 atgggaatgg tatttcagga tttccgtctg ctgaaagacc ggacgatctt tgagaatgta
     4141 gcatttgcac agcaagttgt ggaagtacca caccgatata ttaagcatca ggttgagaat
     4201 atgcttgaga tggtggggct gaaagaccgc atgaatgctt ttccgaatga attgtcagga
     4261 ggagaacagc agagagtggc catcgcgaga gctttggtaa accacccagt attattgctg
     4321 gctgatgagc caactgggaa tctggaccct gtgacagcca aagggatcat ggagcttctc
     4381 tcagagatca ataagagtgg gacaacagtt ttagttgtta cgcacaacaa agagttggtc
     4441 aatgagatgg gcaaacgagt gatcttgatc gaagatggac atatagaaca ggatgagata
     4501 agggggtcct atgatattta gaagattttt gtatggaatc aaagagggca caaggaatat
     4561 tttcaggaat aagatgtttt cattagcatc actgggaacg atcattgctt gtgtcttttt
     4621 gttaggaaca tttttatctg tagcattgaa tgtaaggtca acgatgcagc agatggaaca
     4681 gtctgtagga atttctgttt tttttgaacc aggtatctct gcagaaagaa aagatcagat
     4741 tggggaagaa ataaagaaaa aagaagatat tgccacgatg cggtatgtat ctgctgaaga
     4801 agcatgggaa agctataaga aggatgtatt tcaaggaaat gaagagcttc ttgaaggatt
     4861 cgaagggaac aatccgctaa aagaatcaga ttcctatgag atcacattaa aagaagtaaa
     4921 atcatacaaa caggtagtat cgcatttaga gacgattgat ggtgtcagga aagtccgtta
     4981 ttctgaagga gcggcggaag gaatgatgaa gatcaatact atgggaacgt atcttgctgg
     5041 tgggatcatt ctgatcttaa tggcagtttc tattttcctc gtgagcaata cagtgtctat
     5101 tggagttgca gtcagagaag aggaaatttc aattatgaag ctgatcggtg cggcgaatgg
     5161 atttattaaa gcaccatttc aggcagaagg aatgatcata ggaattttgg gatcattgat
     5221 tccaacagcg attatttttg caggatatga agggatcatc aactggatga atgataagtt
     5281 tcatgttgtt acagtcttta tgaaatttgt accgatccag cagattatgt tatacttact
     5341 tccgttagca ttgttggtag gaatcgtgat cggattgctt ggaagttcta ttacgatccg
     5401 caagtattta aaagtctagg aaggggaact catgaaaaga agatatcagg cggggatcat
     5461 tatgatgtta atatcagcca tgatcgcttc gaatctgcct atgacgacag tgtttgctgc
     5521 agccaaaaaa caacaggtaa agcaggaaac aaagaaatta gaagaacaaa gccgcaagat
     5581 gcagcaggag atcaaagatc ttgatgagaa aatgataaag agcaatgatg cgtatgaggc
     5641 atgtcaagag aagcttattt ctgtacagaa acagttaaag aagacacaac aggaattaaa
     5701 agaggcaaag gcatccaaag aagatcagtc aaggatcatg agtaagcgca ttaagtttct
     5761 atatgaaaat ggaaatatgg catatatgga agtcattttt gaagcaaata attttcagga
     5821 gttcttaaaa agagctgatt atgtttcaaa gatctccaaa tatgacagta acatgttttt
     5881 gcaattacag acaacggagg ataagatccg aatggcaacg aagagtctga aacaggatta
     5941 tcaaaataca aagactttga ctgctaaggc aaagacagaa aaagaaaaac tggatcaggc
     6001 agctgcgaag aagaaatcta agttagcttc atatcagaaa cagttagcat ctgataaaga
     6061 gttactggca tggtttgagg cagaggaaaa acgacaagaa gagatggatc ttgcatcggc
     6121 aaaagacggt aatgcagaca atacgacatc gaaagcacag tcagaaaaga atatgacagg
     6181 aagtacggca tcaaagaata caacatcaaa ggctacgacc gaaagcaaga aagaaactac
     6241 aacgacaacg tctccatcaa caacagtttc gtcaggaaat ctgagttggc cggtaccatc
     6301 ttgtcatagt atttcatcag ggtacggcta ccgaattcat ccagttacag gagtcagaaa
     6361 actgcatgct ggaattgata ttccatgttc aactggaaca acgatcgtag cagcagcttc
     6421 tggtacagta gtggatgctg gatacaatgc atacaatggc aattacttaa agatcagcca
     6481 tggaaatgga ctggaaacga tgtatctgca ttgctccaaa cttctagtat cctcaggggc
     6541 aagagtctca ggaggacaga tgatcgcaaa atccggagca actggtatgg tatctggtgc
     6601 acatctgcat tttgttgtga aaaagaatgg aaattatgta aatcctcaaa attacttata
     6661 acgtcattac ataaatagaa aggaactaat ttcatgaaga agttttcaaa aatactcgta
     6721 gggctacttg ttgtcgtatg tattgtggaa ggattttttc tttatcgtgc ctatgcgatc
     6781 ccaagtacga ccttacagac agcaagaaag attaatcgga tagaacagat c
//
LOCUS       DAOMUE010000082         6765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3010_length_6765_cov_2.139046, whole genome shotgun sequence.
ACCESSION   DAOMUE010000082 DAOMUE010000000
VERSION     DAOMUE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3010_length_6765_cov_2.139046"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /gene="ilvN"
                     /locus_tag="PLC71_05075"
     CDS             complement(<1..485)
                     /gene="ilvN"
                     /locus_tag="PLC71_05075"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRM02045.1"
                     /translation="MRQIVLSILVENTPGVLSRISGLFTRRGYNIDSITACTTQDQKF
                     SRITIAVSGDDMILKQIKNQLSKLEDVVKIFELENSKSVCRELILAKIRANKTEKQEV
                     ITIADIFRAKVVDVSKDNLMIELTGNVNKIEAFINLISDFGVIEIVRTGITGLSRGED
                     NS"
     gene            complement(626..1918)
                     /locus_tag="PLC71_05080"
     CDS             complement(626..1918)
                     /locus_tag="PLC71_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRM02046.1"
                     /translation="MKIKKFKIILSLFLIIGIAAGGVLGYYDARLEAAVNNKNGSTLT
                     DVKVDDNLTFNKNVINILLVGSDHGAIKGDHGRSDSIMIATVNFKTKELKLTSLMRDM
                     YVEIPGHGHNKLNAAYAFGGVELLYQTIAKNFGIKIDNYCVVDFSTFEKVINKVGGVE
                     ISLEEKEAKYLNTTNYISKKKYRNVKVGKQTLNGNQALGYARVRYVVSKKYGDGDFGR
                     TGRQRAVLQAALNKVLQQSPTKIADIALDSLADVSTDMSAKYLKSLVLKVIQMGTTEI
                     DQMRVPLEGTYKMGRAQSNMFVFFINFSANKAAMNYFLFDKGSEKDWAKEYGGISSVE
                     TFGYSGTSSSDSTSSSSSYSTSSTRSSYQQESYSTTSSSHTTYSTRSTTTRSTEERVT
                     ESTTSQPQTSAPRPTTAHHTTTAAATESTPSGDSVEEE"
     gene            complement(2006..2920)
                     /locus_tag="PLC71_05085"
     CDS             complement(2006..2920)
                     /locus_tag="PLC71_05085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM02047.1"
                     /translation="MVIDLIIPTYKPGDKFQESLKRLALQTRKPDRIILVNTEAEFFD
                     EEMITPYDNVEVHHIKKEEFDHGKTRDYGASLAKEADILMFMTDDAIPKDKYMVENLI
                     KAFDDPIVTAAYGRQMADPEKNYIEYYTRIFNYPLESRVKTKEDLDTLGIKTFFCSNV
                     CSAYRRSEYDAMGGFEHKTIFNEDMIMASKMIEDGKAVAYQADARVWHWHDYKAIQQL
                     HRNFDLAVSQVDHGGLFLKVRSESEGVKMVIATIKHLFKKGKIYLIPKCVIDTGFKLI
                     GYKLGRNYKKLPKWMVLKLTMNQTYWKA"
     gene            complement(2944..>3621)
                     /locus_tag="PLC71_05090"
     CDS             complement(2944..>3621)
                     /locus_tag="PLC71_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRM02048.1"
                     /translation="YFVMDRFGYSKTMDKATYQRNKENILKEYKFIVPCMNTDKICIF
                     TNLGVMHQIKVLDIPAGKFRDKGVPIDNLCNYDSTKEYIIQVLDAESMIHSSLLFATA
                     KSMLKVMDGELLNAAKRTVQATKLSEDELIEVIPIDWMEGSEDKVVLQTKEGVFLKFL
                     LSEIPKKKKSALGVRGMKLSKDDSLTNIYVMAAQNISSIVYKEKELEFHRLKLSKRDS
                     KGTKVRR"
     assembly_gap    3622..3631
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3632..5191)
                     /locus_tag="PLC71_05095"
     CDS             complement(<3632..5191)
                     /locus_tag="PLC71_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRM02049.1"
                     /translation="MSEHIIKAEFSEVMQKSYIDYAMSVICARALPDARDGLKPVQRR
                     VLYSMDELGLRYDRPHRKSARIVGDTMGKYHPHGDSSIYESLVVMSQDFKKGVPLVDG
                     HGNFGSIEGDGAAAMRYTEARLQKITQEAYLADLDKNVVDFVSNFDETEKEPEVLPVK
                     VPNLLVNGADGIAVGMTTSIPPHNLGEVVDAVKAYMRKESITNEELMEYIEGPDFPTG
                     GLVINKKDLLNIYSSGTGKLKLRGKVTFEPAKKRGEKDKLVISEIPYTMIGNNISKFL
                     SDTVALIENKVTTDILDISNESSKDGIRIVLELRKGADVERLKNLLYKKTKLEDTFGV
                     NMLAIVDGRPETLDLKGIIENHTQFYYEVTRRKYKTLLSKLYEQREIKEGLIKACDMI
                     DLIIEIIRGSKSQKEVKACLTKGDVGNINFKSSDSMIEATKLSFTDKQASAILDLKLY
                     RLIGLEILMLKEEYEEILAKIEEYEDVLNNEKSLKNVIRKELDKIKKEFGRERRTVVE
                     DGKEAVYVAAPV"
     gene            complement(5213..>5429)
                     /locus_tag="PLC71_05100"
     CDS             complement(5213..>5429)
                     /locus_tag="PLC71_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005360050.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRM02050.1"
                     /translation="GVEVQRYKGLGEMNPEQLWETTLNPESRTLKQIEIEDARMASDI
                     TSMLMGSDVPPRRQFIYENANEAMLDI"
     assembly_gap    5430..5439
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5440..>6765)
                     /locus_tag="PLC71_05105"
     CDS             complement(<5440..>6765)
                     /locus_tag="PLC71_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRM02051.1"
                     /translation="YERGKIITPLEVIGTCRKSDTGTNVQFLPDPEIFDKTYFKAAMV
                     RNRLHETAYLNPKLTIHYVNEREGEETTVDYHEPEGICAYVKKLNEGKQMLHEPVYFK
                     RMIDGIEAEVAFQYTEDFGENILGFCNNIYTIEGGTHITGFKTKFTTIMNQYAREIGV
                     LKEKDSNFTGTDVRNGMTAIVSVKHPEPRFEGQTKTKLDNPDAAKAVGEIAGEELVLY
                     FDKNLDTLKKVLACAEKSAKIRKAEEKAKTNMLVKQKLSIDSNGKLANCESRKAQDCE
                     IFIVEGDSAGGSAKTARNRKTQAILPIRGKILNVEKATMDKVLANAEIKTMINAFGCG
                     FSEGYGNDFDISKLRYHKIIIMTDADVDGAHIATLLLTFFYRFMPELITHGHVFLATP
                     PLYKAMPKKGEETYLFDDAALTRYRAEHKGNFTLQRYKGLGEMDAQQLW"
BASE COUNT         1783 a         1492 c          986 g         2484 t
ORIGIN      
        1 gagttatctt ctcctcttga taaaccagtg attcctgttc tcacaatttc gatcactcca
       61 aaatcactga tcagattgat aaatgcttct atcttattca cattcccagt taactcgatc
      121 atcagattat ccttagatac atccacaact ttcgctctga aaatatctgc aatcgtgata
      181 acctcctgtt tctctgtctt gtttgcacgg atctttgcaa ggatcaattc tctgcaaaca
      241 gacttactgt tttccagttc aaaaatcttc acaacatctt ccagttttga aagttgattc
      301 ttgatctgtt tcaagatcat atcatcaccg ctgactgcga tcgtaattct tgaaaacttc
      361 tgatcttgtg tcgtacatgc tgtgatgctg tctatgttat accctcttct tgtaaataaa
      421 ccagaaattc ggcttaatac cccgggagta ttctctacta aaatagaaag aacgatctgt
      481 cgcatatctt ttatttcctt tctttgtctt attctatcac cgtaatggta tattacaggg
      541 tattggtaac tttttttatt ctaccaatta tacaaatatg tgtcaagaaa gaatatgata
      601 tgacagtgat tctaccttga cacatttatt cttcttcaac agaatctcca gaaggtgttg
      661 attctgtcgc cgcagctgtc gttgtatgat gtgctgtggt tggtcttggt gcagatgtct
      721 gtggctgtga cgttgtactt tctgtcacac gttcttctgt acttctcgta gttgtagacc
      781 ttgtactata agtcgtatgg ctgctgcttg ttgtgctgta actttcctgc tgatagctgc
      841 ttcttgtact agatgttgaa tatgatgaac tactgcttgt tgagtcagat gatgatgttc
      901 cggaatatcc aaatgtctca acagaagata ttcctccata ttcttttgcc cagtcttttt
      961 cactgccttt gtcaaataag aaataattca tggctgcttt attggcactg aagtttataa
     1021 agaatacgaa catattgctc tgtgctcttc ccatcttata agtaccttct aatggcaccc
     1081 tcatctggtc gatttctgtc gtccccatct gaataacttt caataccaga ctcttcaggt
     1141 attttgcact catatctgtt gacacatcgg ctaatgaatc taacgcaata tctgcgatct
     1201 ttgttgggct ctgctgcaat actttattaa gtgctgcttg aagtactgct ctttgacgac
     1261 ctgttcttcc aaagtctcca tctccgtatt tcttagaaac aacgtaacga accctggcat
     1321 atcctaatgc ctgatttcca ttcaatgtct gtttcccaac ctttacatta cggtatttct
     1381 tctttgaaat gtaatttgtt gtattcaagt attttgcttc tttttcttcc agcgaaattt
     1441 ctacaccgcc aactttattg ataactttct caaatgttga gaaatcaaca acacaataat
     1501 tgtcgatctt aatcccaaag ttcttagcga tcgtctggta taataattct acaccaccaa
     1561 atgcataggc tgcatttaac ttgttatgtc catgtcctgg aatctcaaca tacatatctc
     1621 tcatcaaaga agtgagcttt aattcttttg tcttaaaatt gaccgttgcg atcataatac
     1681 tgtcagatct tccgtgatca cctttgattg ctccatgatc agatccaact aataagatat
     1741 taataacatt cttattaaat gtcagattat catctacctt tacgtctgtc agtgtacttc
     1801 cattcttatt attcactgct gcttctaatc ttgcatcata atatccaagc actccccctg
     1861 cagcaattcc tatgatcaaa aacagactta ggataatttt aaacttctta atcttcattc
     1921 gtgtcctcct cacaattatc tgcttatcat tgtagattat atcatatcat acaaaaaaac
     1981 agtaggtgat tttattaagt ttttgttaag ctttccaata agtctgattc atcgtaagct
     2041 ttaaaaccat ccattttggc agttttttat aatttcttcc aagtttatat ccgatcaatt
     2101 taaatcctgt atcaatcaca cactttggaa taagatatat ttttcccttt ttaaaaagat
     2161 gcttgatcgt cgcgataacc atttttacac cttccgattc tgatctcact ttaagaaaca
     2221 atcctccatg atctacttgt gacactgcaa gatcgaaatt cctatgtaac tgttgtatcg
     2281 ccttgtaatc atgccaatgc catacccttg catctgcctg ataggcaact gcctttccat
     2341 cttcgatcat ctttgaagcc atgatcatgt cctcgttaaa aattgtttta tgctcgaagc
     2401 ctcccattgc atcatactct gatcttcgat aagcactgca tacatttgaa cagaaaaatg
     2461 ttttgatccc cagagtatcc agatcctctt ttgttttaac tctgctttct agaggataat
     2521 taaagattct tgtataatac tcaatataat ttttttcagg atcagccatc tgccttccat
     2581 aagcagctgt tactattgga tcatcaaatg ctttaatcag attttccacc atatatttat
     2641 cttttggaat cgcatcatct gtcataaaca ttaaaatgtc agcttctttg gcaagcgatg
     2701 ctccataatc tcttgttttc ccatgatcaa attcttcttt tttaatatga tgaacttcta
     2761 cattatcata tggagtgatc atttcctcgt caaaaaattc tgcctctgtg ttaactaaaa
     2821 taatgcggtc tggttttctt gtctgcaatg caagtcgttt taatgattcc tgaaacttat
     2881 ctcctggctt atatgttggt atgatcaagt caattaccat gatttttcct ttctatctta
     2941 gatctatctg cggactttag ttcctttgct atctcttttt gatagtttta accgatgaaa
     3001 ttccagttct ttttctttat atacgataga actgatattc tgtgctgcca tcacataaat
     3061 attcgttagt gaatcatctt ttgataactt cattccacga acacctaatg cactcttctt
     3121 cttttttgga atctcggaaa gcaagaattt taagaaaact ccttctttcg tctgaaggac
     3181 tactttgtct tcggatcctt ccatccagtc tatcggaatc acttcgatca gctcatcttc
     3241 cgataatttt gttgcctgca cagttctctt tgctgcattt aacaactctc catccatcac
     3301 ttttaacata ctttttgctg ttgcaaataa aagtgatgaa tgaatcattg attctgcatc
     3361 cagcacctgg atgatatatt cttttgtact gtcataatta cataaattat caattggtac
     3421 ccctttatcc ctgaattttc cggctggaat atccagtacc ttgatctggt gcatcacacc
     3481 gagattggta aagatacaga tcttatctgt attcatacaa ggcacaataa acttgtattc
     3541 ttttaagata ttttccttat ttctctgata agttgcctta tccattgtct tggaatatcc
     3601 aaaacgatcc atcacaaaat annnnnnnnn ngactggtgc agcaacataa actgcctctt
     3661 ttccatcttc gaccacagtt cttctttcgc gtccaaattc ttttttaatc ttatctaatt
     3721 ctttacgaat cacgttcttt aaagatttct cgttatttaa gacatcttca tattcttcga
     3781 tctttgcaag aatctcttca tattcttctt ttaacatcaa aatctctaat ccgatcaatc
     3841 ggtataattt aagatcaagg atcgcacttg cctgtttgtc tgtaaaagaa agcttggttg
     3901 cttcgatcat agaatctgaa gacttgaaat taatatttcc aacatctcct ttggtaaggc
     3961 atgcctttac ttccttctga ctcttacttc cacgaatgat ttcaatgatc agatcaatca
     4021 tatcacatgc tttgatcaac ccttctttaa tttctctttg ttcatataat tttgatagta
     4081 atgttttata ttttcttctt gttacttcat aataaaactg cgtatgattt tcaatgattc
     4141 ccttaagatc cagtgtctcc ggacgtccat caacgatcgc cagcatattc acaccaaatg
     4201 tatcctcaag ttttgttttc ttatataaga gattttttaa gcgttcaaca tctgcaccct
     4261 ttcgaagttc taatacaatt cggattccat ctttagagga ttcattggaa atatccaaaa
     4321 tatctgttgt cactttgttc tctatgagtg ctacggtatc tgataaaaac ttggaaatat
     4381 tatttccgat cattgtgtat ggaatttctg aaattaccag tttatctttt tccccacgtt
     4441 tctttgctgg ttcaaaagtt acttttcctc ttaattttaa tttacctgtt cctgaagaat
     4501 aaatatttaa caaatctttc ttattgatca caaggccgcc tgttggaaaa tccggacctt
     4561 ctatatattc catgagttct tcgtttgtga tgctttcttt tcgcatatat gctttcactg
     4621 catcaacaac ttcccctaaa ttatgtggtg gaatactcgt tgtcatacca acagcgattc
     4681 catctgctcc atttaccaat aagtttggca ccttaactgg aagtacttca ggttcttttt
     4741 ctgtttcatc aaagttcgat acaaaatcaa ctacattttt gtcaaggtct gcaagatatg
     4801 cttcctgcgt gatcttctga agtcgtgctt ctgtatatcg catagccgca gcgccgtctc
     4861 cctctatgga accaaagttt ccatgtccat caacaagagg gactcctttt ttgaaatcct
     4921 gagacataac aactagggat tcgtagatgg aactgtcacc atgtggatga tatttaccca
     4981 tggtatcccc gacgattctt gcagattttc gatgtggcct gtcataacga agtcctaatt
     5041 catccatgga atataaaact cttctctgta ctggctttaa tccatctctg gcatctggaa
     5101 gtgctcttgc acaaataaca ctcatcgcat aatcgatata tgatttttgc ataacctcag
     5161 aaaattctgc tttaataata tgttctgaca ttatctattg tttcctttct atttatatat
     5221 ctaacatcgc ttcatttgca ttttcataaa tgaactgtct gcgaggtggt acgtcgcttc
     5281 ccatcagcat agaagtaata tcggaggcca ttctagcatc ttcgatctca atctgtttta
     5341 atgtacgact ttctggattc agcgttgtct cccatagttg ctcaggattc atttccccaa
     5401 gacctttgta acgctgtact tcaacacctn nnnnnnnnnc ccataactgt tgcgcatcca
     5461 tctctcctaa acctttataa cgttgtaaag taaagtttcc cttatgttct gcacgatatc
     5521 ttgtcagtgc tgcatcatca aacagataag tttcttcgcc ctttttgggc attgctttgt
     5581 aaagcggcgg tgttgcaaga aatacatgtc catgcgtgat caattctggc ataaaacgat
     5641 aaaagaacgt caaaagcaat gttgcgatat gtgcaccatc cacatcagca tctgtcataa
     5701 taatgatctt gtgataacga agcttactga tatcaaaatc atttccatat ccttcagaaa
     5761 aaccacatcc aaatgcattg atcatcgttt taatttctgc attcgcaaga actttatcca
     5821 tcgtagcttt ctctacattt aagatttttc ctcgaattgg aaggattgcc tgtgtctttc
     5881 ggtttcttgc tgtttttgca gaaccaccag cagaatctcc ctcgacaata aaaatctcac
     5941 agtcttgtgc tttacgactt tcacagttcg ccagttttcc gttgctgtca attgacaact
     6001 tctgttttac cagcatattc gtcttcgctt tttcttccgc tttacggatc ttcgctgatt
     6061 tttccgcaca tgccagcact ttttttaaag tatctagatt cttatcaaaa taaagaacca
     6121 attcttctcc cgcgatctct ccaactgctt tggctgcatc tggattatcc agtttcgtct
     6181 tcgtctgtcc ttcaaatctt ggttctggat gtttgacgga aacgattgct gtcataccat
     6241 ttctcacatc tgttccagta aaattcgaat ctttttcttt taataccccg atctctctgg
     6301 catattgatt catgatcgta gtgaatttcg tcttaaaccc tgtgatatgt gttccacctt
     6361 cgatcgtata aatgttatta caaaatccca gaatattctc cccaaaatcc tctgtatact
     6421 gaaatgccac ttctgcctca attccatcaa tcattctttt aaaatatact ggttcatgaa
     6481 gcatttgttt tccttcattt aatttcttta cataagcgca gattccttct ggctcatgat
     6541 aatcaactgt tgtttcttca ccttctcgtt cattcacata atgaatcgta agttttggat
     6601 tcaaatacgc agtctcatgc aaacggtttc taaccattgc tgctttaaaa tatgtcttat
     6661 caaaaatttc tgggtctggt aaaaactgga cgtttgttcc cgtatctgat tttctacagg
     6721 taccgatcac ctcaagaggt gttatgatct ttccacgctc atatc
//
LOCUS       DAOMUE010000083         6740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3022_length_6740_cov_2.418549, whole genome shotgun sequence.
ACCESSION   DAOMUE010000083 DAOMUE010000000
VERSION     DAOMUE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3022_length_6740_cov_2.418549"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1072
                     /locus_tag="PLC71_05110"
     CDS             185..1072
                     /locus_tag="PLC71_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRM02052.1"
                     /translation="MKYAVGIDVGGTNTRVALINEKYEIKERVQFGSDPKNPIKTLNQ
                     INDVIKGFGEKIEGIGISCPGPLDLINGIILTPPNLPGWHNFELTKELEKITGISVQL
                     ENDANLAGLAETVIGAGKGKKIVEFLTISTGVGAGLCIDGQIYRGAKGFAQEVANCIL
                     WKNGPSQGDLKKGSIESIASGTAITKRANDAGLEAAHAGEVYQLAQEGNETAAMIMED
                     AYEYLSSFIATLYGVLDPALFVLSGSVALKIPGFIEEIEKRAKEKVYDALKSNVKIVP
                     AALGEDCGLIGAACLAFDN"
     gene            1177..2043
                     /locus_tag="PLC71_05115"
     CDS             1177..2043
                     /locus_tag="PLC71_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM02053.1"
                     /translation="MTLKNMIELADGLTPIETEIGYYILKNQEDILDMSVIELAEKTF
                     VSKSAIHRFCKKIGLKGFNELKVILAKDISEMKSDIEMIDVNYPFERKDGPQMIAKKL
                     SKLYETAIYDTYDYMDFIELHKVAQLLHKAEVIDIYTHSHNMNVAENFQDKMLTAGKS
                     SVECPHGFYKQRLTALASNPKHAAIILSYSGKATFVKPILKVLHQKKVPVVYVGKAGG
                     NLYPQYVAGALTISSRENLRDRISQFSSHIAMQYMMDVVFGCIYNMDREKNIEYLKES
                     IGFMDDRDIQDE"
     gene            complement(2051..2911)
                     /locus_tag="PLC71_05120"
     CDS             complement(2051..2911)
                     /locus_tag="PLC71_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM02054.1"
                     /translation="MIDMILEQIKNPDFFNNSEQNMIYFLQNNLNSFDQVTLNKMAKE
                     SFSSTSSVLRFCKKLGFDGFKDFKLQLIREASISGQDLNFNLPFSPDDSVEDIGLHLS
                     EILANGIKKTRNLLDPVQLEMTAYNIKKSHRVFLFGKGDSRISLENFKNKMIKLNEYF
                     IIGDEYQNNFYTVGNITSNDLVIFCTYSAMHHDYNSYLPVLKDHNVPIITITSNIHSK
                     LAVNSDIVLQLPDDESFDEKVASFASQTNMDFIFDYIYSIIFQKNYMENYHSKHAKDV
                     YTNSMIYNKL"
     gene            3108..4436
                     /locus_tag="PLC71_05125"
     CDS             3108..4436
                     /locus_tag="PLC71_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HRM02055.1"
                     /translation="MKKEFKIVICGGGSTYTAGIVKNLLEEEELKIKELWLYDIDQER
                     QEKVSLIVKEVVKDLRPSLELKISTDEEEAFTDADFIMAQMRVGGLKMRVKDEQISLK
                     HGCIGQETCGAGGMAYGMRTIGPMVHLIDVCEKYASKTYWIVNYSNPAAIVAKATQTL
                     RPNARILNICDMPVEVEARMAEILDTDLSNLEVDYFGLNHYGWFTKVQCNGEDVTEKL
                     KKHVAEYGYVSKASYEDALVKDPDWLHTFTNAKKIVNYFPDYLPNTYWQYYLLGDDIV
                     DYMDINNTRGMQVIHGRETKIREAVKKLENGEKIDLTQFYVGVHGKFIVEVVKALAYD
                     TRSRQLVIVKNDGAVKNLPDDAMVEIPAYITKDGPEPVRVGEIPRFYKGLIEQQDACE
                     GLVVEAVIEGSYKKALQAFTLNRTIPSANVAKEILDEMIEANKEYWLELK"
     gene            4588..5976
                     /locus_tag="PLC71_05130"
     CDS             4588..5976
                     /locus_tag="PLC71_05130"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HRM02056.1"
                     /translation="MKGTVKIVTIGGGSSYTPELMEGFIKRYEELPIKEIWLVDIEDG
                     KEKVEIVGALAQRMWDASPYDVKVYTTLDRREALKDADFVTTQFRVGLLDARIKDERI
                     PSLYGMLGQETNGAGGMFKAFRTIPVIGQIIEDMRELCPDAWLINFTNPSGMVTEAVI
                     KHFGWKKCIGLCNVPTIAMMAEPKLLGKDINQLNYRYVGINHFHWHKVFDENGNDMTP
                     ILIDHINEKGGGTPANIYQAEFPLELLHSMNMVPCGYHRYYYMKQAMLEHAIEEFNEG
                     GTRAEQMKQVEHELFEIYKNAELHEKPEQLGKRGGAYYSDAACECIRAIYANKKIHMV
                     VSTQNNGAISCLDDDSIVEVSSIISATGAQPMAFGKLPSAEKGWLQMMKAMEECTIEA
                     ALTGDYGKALEAFVLNPLVENNENTTKVLDELLVAHAKYLPQFKEKIEELKAKGVHST
                     DPVVMDLMEHGH"
     gene            5990..>6740
                     /locus_tag="PLC71_05135"
     CDS             5990..>6740
                     /locus_tag="PLC71_05135"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRM02057.1"
                     /translation="MGVTKESFGFLEDKREAFLYTIENQNGFAVKVTDFGVNIVSLLV
                     KNKEGNIKDVALGYDTLEEYFENGIMFGATVGRNVNRISNARFEIDGKEYHVAKNRGK
                     HNIHSDKEHGFHKVLWDSEIIDDHAVKFTYVSPDGEQGFPGTLVTNIIYTVTETNGLI
                     VSYHAVSDKKTLINLTNHNYFNLGGHESGTIENTKVRIYADEFTPINEDTIPTGEIRK
                     VEHTPMDFREWKKIKNDLYDEDEQLEKGKGYD"
BASE COUNT         2531 a          936 c         1426 g         1847 t
ORIGIN      
        1 cctcttgaaa tctatccaga accaatcaaa gcttgtagag aagttattgc tcacaaagtt
       61 aacttatttg attctgcaga caaagttaaa tattactacg aataagatat ttacaaacaa
      121 caacataaag aggggacagc ttcggttgtc tcccttttcg tttatatagg agggaaagaa
      181 aatcatgaaa tatgcagtag gaattgatgt tgggggaaca aatacaagag ttgcgctgat
      241 caacgagaaa tatgagatca aagaaagagt tcagtttgga tcagatccaa aaaatcctat
      301 aaaaacatta aaccagatca atgatgtgat caaaggattt ggagaaaaaa tcgaagggat
      361 cggaatttct tgtccagggc cattagactt gatcaatggg attattttaa caccaccaaa
      421 ccttccaggg tggcataatt ttgaactgac aaaagaatta gaaaagatta caggtatttc
      481 tgtgcagtta gaaaatgatg cgaaccttgc aggacttgca gaaactgtga tcggggctgg
      541 aaaaggaaag aagatcgtag aatttctgac catttcaaca ggagttggtg caggattatg
      601 tattgatgga caaatctatc gtggagcaaa aggatttgca caagaagtag caaactgcat
      661 tttatggaaa aatggtccat cacagggaga tttaaagaaa ggttccattg aatcaattgc
      721 cagtggaaca gccatcacaa agagggcaaa tgatgcaggg ttagaagctg cccatgcagg
      781 agaagtgtat caacttgcac aggaaggaaa tgaaactgcg gcaatgatca tggaagatgc
      841 ttatgaatat ctgtcaagtt tcattgcaac actttatggt gtacttgatc cagcgctatt
      901 tgtcctaagt ggaagtgttg cgttaaagat tcctggattt atcgaagaga ttgaaaaacg
      961 cgcaaaagag aaagtatatg atgcgctaaa aagcaatgtg aagattgtgc cagcggcgtt
     1021 gggagaagat tgcggtttga ttggagctgc atgtttggct tttgataatt agatatagtt
     1081 tttgggtaga agtaataaat tatttgagat aataaaatag caatgttaaa ataataagga
     1141 acactgaaat aaatcaaaaa taaagaggtg ctgcctatga cacttaaaaa tatgattgaa
     1201 ttagcagatg gattaacacc aattgaaaca gaaataggat attatattct aaaaaatcaa
     1261 gaagatatct tagatatgtc ggttattgaa ttagcagaaa aaacatttgt gtcaaaatca
     1321 gccattcatc gattttgcaa aaagattggg ttaaaaggat tcaatgaatt gaaagtcata
     1381 ttggcaaaag atatctcaga aatgaaatca gatattgaga tgatcgatgt aaattatcca
     1441 tttgaacgaa aagatggacc acagatgatc gcaaagaaac tgagtaagtt atatgaaaca
     1501 gcaatctatg atacctatga ttatatggat tttatagaat tgcataaagt ggcacaatta
     1561 ttacataaag cggaagttat agacatttat acccattcac ataacatgaa tgttgcagaa
     1621 aattttcagg ataagatgtt aacagcaggc aaaagtagtg tagaatgtcc acatggattt
     1681 tataaacaaa gactgacagc attagcaagt aatccgaaac atgcagcgat cattctctca
     1741 tattctggaa aagcaacctt tgtaaagcca attttaaagg tgctgcatca aaagaaggtg
     1801 cctgttgtgt atgttgggaa agcaggtggg aatttatatc cacagtatgt tgccggtgca
     1861 ttaacgatca gcagcagaga aaacttaaga gatcgtatct ctcagttctc atctcatatt
     1921 gcgatgcagt atatgatgga tgttgttttt ggctgtattt acaatatgga ccgtgaaaag
     1981 aatattgaat atctcaaaga atccatagga tttatggatg atcgagatat tcaggatgaa
     2041 tgatactaaa ttatagtttg ttatagatca tggaatttgt atatacatct ttagcatgtt
     2101 tcgaatgata attctccata taatttttct ggaaaatgat agaatagatg taatcaaaaa
     2161 taaaatccat atttgtttga gaggcaaaac tggcaacttt ttcatcaaag gattcatcat
     2221 caggaagttg taatacaata tcagaattca cagccagttt ggaatgaata ttacttgtaa
     2281 tcgtaatgat cggaacgtta tgatctttta aaactggaag ataagaattg taatcatgat
     2341 gcatagcact ataagtacag aagatcacaa ggtcattaga ggtaatattg ccgactgtat
     2401 aaaaattgtt ctgatattca tcaccaataa taaaatattc atttaatttg atcattttat
     2461 ttttaaaatt ctcaagagaa attctggagt caccttttcc aaacaggaat acacggtggc
     2521 tcttttttat attataagca gtcatttcta attgcacggg atctaacaga ttccgtgttt
     2581 ttttaattcc atttgcaaga atttcagata aatgaagacc gatatcttca acagaatcat
     2641 ctggagaaaa aggtagatta aaattaagat cctgaccaga aatagatgct tctcggatca
     2701 gttgtaattt gaaatcttta aatccatcaa atcctaattt tttgcaaaaa cgtaaaacag
     2761 aagaagtcga agaaaaggat tcttttgcca ttttgtttaa agtgacttga tcaaaagagt
     2821 ttaaattgtt ttgtaaaaaa taaatcatat tctgttcaga attattaaaa aaatcaggat
     2881 ttttaatttg ttctagtatc atatcaatca ttctcctaaa aaagtatagt tttcaaatat
     2941 ttcttttttt attgtgaatt tgcacaaata accggaataa atttatgcag attgtccatg
     3001 aaatagttgt gatgttccaa aaatagcgtg aactattgag tctgaaattt tcaagaaata
     3061 taatcagggc acatgaaata atatgacgga tatggaggaa aaaatcaatg aaaaaagaat
     3121 ttaagattgt gatatgtggt ggtggaagta cttataccgc agggattgta aaaaatcttt
     3181 tggaagagga agaattaaaa atcaaagaat tatggttata tgatattgat caggaacgac
     3241 aagaaaaagt gtcactgatc gtaaaagaag ttgtaaaaga tttaagacct tctttggaat
     3301 tgaaaattag tacggatgaa gaagaggcat ttacagatgc agactttatt atggcacaga
     3361 tgcgagtcgg tggactgaaa atgcgagtaa aagatgaaca gatttcatta aaacatggat
     3421 gcattggaca ggaaacatgt ggagccgggg gaatggcata tggaatgaga acgatcggac
     3481 caatggtaca tctgatcgat gtttgtgaga aatatgcatc aaaaacttat tggatcgtta
     3541 attattcaaa tccagctgcg attgttgcta aggcaacaca gacattacgt ccaaatgcaa
     3601 gaattttaaa tatctgtgat atgccagttg aggtagaggc aagaatggca gaaatcctag
     3661 acacagatct tagcaatctg gaagtggatt attttggatt gaatcattat ggatggttta
     3721 caaaagtaca gtgtaatggc gaagatgtca cagaaaaatt aaagaaacat gtggcagagt
     3781 atggttatgt atcaaaggca agttatgaag atgccttggt caaagatcca gactggttac
     3841 atacatttac gaatgcaaag aagatcgtca actattttcc agattatctg ccaaatacat
     3901 actggcagta ttatcttctt ggagatgaca ttgtagatta catggatatt aataatacaa
     3961 ggggaatgca ggttattcat ggaagagaaa caaagattcg tgaggcagtt aagaaactag
     4021 aaaatggaga aaaaatcgat ttgacacaat tttatgtagg agttcatggg aaatttattg
     4081 tagaagtggt aaaagcactt gcatatgaca cgagaagcag gcagcttgtc atcgtaaaaa
     4141 atgacggagc agtcaagaat cttccagatg atgccatggt tgaaattcct gcatatatta
     4201 caaaagacgg accagaacca gtaagagttg gagaaattcc aagattttat aaaggactga
     4261 tcgaacagca ggatgcttgt gaagggttgg tcgtagaagc agtgatcgaa ggatcatata
     4321 aaaaagcatt gcaggcattt actttaaacc gcacaattcc atctgcaaat gtggcaaaag
     4381 aaattttaga tgaaatgatc gaagcaaaca aagaatactg gctggaattg aaataaagtt
     4441 tcaaaaagtt tcagaaattt tcatgattct gaaacttttt ttgggaaaaa atatccgcat
     4501 ctactaaatc cattgtgaga aaaaagctga ctctatatga tgtaaacaga acaaagaaaa
     4561 cgaacatatt tgaggaggca tagaacaatg aaaggtacag tgaagatcgt aacgattggt
     4621 ggaggaagta gttacacacc agagctgatg gaaggtttca tcaaacgata tgaagaactt
     4681 ccaattaaag aaatctggct ggtcgatatc gaagatggaa aagaaaaagt agagattgta
     4741 ggagcactgg cacagagaat gtgggatgca tctccatatg atgtaaaagt ttatacaaca
     4801 ttagacagaa gagaagcatt aaaagatgca gactttgtta caacacaatt tcgagtaggc
     4861 cttttagatg ccagaattaa agatgaaaga attccatcat tgtatggaat gcttggtcag
     4921 gaaacaaatg gagcaggagg aatgtttaaa gcatttcgaa ccattccagt gatcggacag
     4981 atcatagaag atatgagaga attatgccca gatgcatggc tgattaactt tacaaatcca
     5041 agtggaatgg taacagaagc tgtaattaaa cattttggat ggaaaaaatg tatcggactt
     5101 tgcaacgtgc caacgatcgc aatgatggca gaaccaaagc tccttgggaa ggatatcaac
     5161 cagttaaatt acagatatgt aggaattaac cattttcact ggcataaagt atttgatgaa
     5221 aatggaaatg atatgacacc aattttgatc gatcatatca atgaaaaagg tggtggaaca
     5281 ccagccaata tctatcaagc tgaatttcca ttggaattac ttcacagcat gaatatggtg
     5341 ccttgtggat accacagata ttactacatg aaacaagcaa tgcttgagca tgcgattgaa
     5401 gaattcaatg aaggtggtac tagagcagag caaatgaaac aggtagaaca tgagttgttt
     5461 gaaatttata aaaatgcaga acttcatgaa aaaccagaac aacttggaaa acgtggagga
     5521 gcatattaca gcgatgcagc ttgcgaatgt atccgtgcaa tttatgcaaa taagaagatc
     5581 catatggtag taagtacaca aaacaatgga gcaatttcat gcttagatga tgactctatc
     5641 gtggaagtat caagcatcat ttcagcgaca ggagcacagc caatggcatt tggaaagctt
     5701 ccatcagcag aaaaaggatg gcttcagatg atgaaagcaa tggaagaatg tacaattgaa
     5761 gcggcattga caggagatta cggaaaagca ttagaagcct tcgtattaaa tccattagta
     5821 gaaaataatg aaaatacaac aaaagtttta gatgaactgt tagttgcaca tgcaaaatat
     5881 ctgccacagt ttaaagaaaa gattgaagaa cttaaagcaa aaggagttca ttctacagat
     5941 cctgttgtta tggacttaat ggaacatgga cactaagcag gaggctgata tgggtgtaac
     6001 aaaagaaagt tttggctttt tagaagataa aagagaagca tttctataca cgattgaaaa
     6061 tcaaaatgga tttgcagtca aagttactga ttttggtgta aatattgttt ctctccttgt
     6121 aaaaaacaag gagggaaaca tcaaagatgt agcccttgga tatgacacct tggaagaata
     6181 ttttgagaat ggaattatgt ttggagcaac tgtcggacgg aatgtaaacc gtatctcaaa
     6241 tgccagattc gaaattgatg ggaaagagta tcatgtagca aaaaatcgtg gaaaacataa
     6301 cattcacagt gacaaagaac atggatttca caaagtctta tgggatagtg agatcattga
     6361 tgatcatgca gtgaagttta cttatgtaag tccagatggg gaacaaggat ttccgggaac
     6421 attggtaaca aatatcattt atacggtaac ggaaacaaat ggattgatcg tttcctatca
     6481 tgcagtcagc gataagaaaa cgctgatcaa tctgacaaat cataattact ttaacttggg
     6541 tggacatgaa agcggaacga tcgaaaatac aaaagtaagg atttatgcag atgaatttac
     6601 accgatcaat gaagatacga tcccaacagg agaaatcaga aaagtagaac atacaccgat
     6661 ggatttccgt gaatggaaaa agatcaagaa tgatttatat gacgaagatg aacaactaga
     6721 aaaaggcaaa ggatatgatc
//
LOCUS       DAOMUE010000084         6736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3027_length_6736_cov_2.708427, whole genome shotgun sequence.
ACCESSION   DAOMUE010000084 DAOMUE010000000
VERSION     DAOMUE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3027_length_6736_cov_2.708427"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLC71_05140"
     CDS             <1..374
                     /locus_tag="PLC71_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRM02058.1"
                     /translation="PISAIAERQNISVRYLEQLLPKLKKAGIIKSIRGAQGGYMLDKD
                     PKDISAGDILRTLEGDLTPIDCAAFTGDTSTCSGTKFCVTKTVWKRINDSVQQAVDSI
                     TLQELMEESIEIHTKYDQEER"
     gene            386..1570
                     /gene="nifS"
                     /locus_tag="PLC71_05145"
     CDS             386..1570
                     /gene="nifS"
                     /locus_tag="PLC71_05145"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HRM02059.1"
                     /translation="MEKKIIYLDHAATTATRPEVVDAMLPYFTENFWNPSSVYTPALN
                     NRKVVDESRETIARSLGTTPENIYFTAGGSESDNWALKCTAEAYANKGKHIITSKIEH
                     HAILHTCEYLEKKGFEITYIDVDENGILKLDDLKAAIRPDTILISVMFANNEIGTIQP
                     IKEIGAIAKEHGILFHTDAVQAYAQIPINVDEYNIDMLSVSGHKFGGPKGIGFLYIRK
                     GLKLRSFIHGGAQERKRRAGTENITGIVGIAKAVEIAFATMDERIKHETEVRDYAIKR
                     ILAEVPYCRLNGDAQKRVPNNINISFQFIEGESLLIMLDMAGICASSGSACTSGSLDP
                     SHVLLAIGLPHEIAHGSLRMTIGEETTKEDMDYVVDNIKEIVTKLRTMSPLYEDFVKK
                     NN"
     gene            1645..2082
                     /gene="nifU"
                     /locus_tag="PLC71_05150"
     CDS             1645..2082
                     /gene="nifU"
                     /locus_tag="PLC71_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HRM02060.1"
                     /translation="MYSEKVMDHFQHPRNVGEIENASGVGTVGNAKCGDIMRIYLDID
                     DNGIIQDVKFKTFGCGAAVATSSMATELVKGKNIQQALEVTNKAVMEALDGLPPVKVH
                     CSLLAEEAIHAALWDYAEKNGIKIEGLEKPKTDISEGEEEEEY"
     gene            2092..3183
                     /gene="mnmA"
                     /locus_tag="PLC71_05155"
     CDS             2092..3183
                     /gene="mnmA"
                     /locus_tag="PLC71_05155"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRM02061.1"
                     /translation="MKEKVVVGMSGGVDSSVAAYLLKKQGYDVIGVTMQIWQEEDEFV
                     QEENGGCCGLSAVDDARRVAQMIGIPHYVMNFRKDFKDKVIDYFCEEYLNARTPNPCI
                     ACNRYVKWESLLKRCLDIGADYIATGHYSQITKLDNGRYALKCSKTQKKDQTYALYNL
                     TQNQLSKTLMPIGAYTKDEVRQIAEEIGLLVANKPDSQDICFVDDNYGEFIEETTGTK
                     IPKGNFVDPEGNVLGEHKGIIYYTVGQRRGLGLSLKKPGYVIKVDKDTNEVVVGDKQD
                     LFANVLYGNKVNCMSVPEFEDGMKLMAKIRYNSPEVPCRVYMTGEDEVRVVFDEPQRA
                     ITPGQAVVFYDGDIVAGGATIFRAANENM"
     gene            3271..4140
                     /locus_tag="PLC71_05160"
     CDS             3271..4140
                     /locus_tag="PLC71_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HRM02062.1"
                     /translation="MLNFIRGFCMALADSVPGVSGGTVAFLLGFYDKFIMSLDHLMSG
                     TKEERIEAIKFLIRIGIGWVVGMGAAVSVLASIFDDRIYEISSLFLGLIIFAIPMICK
                     EEKDSLKGKYQNIIWAVIGVVLVAAITYFNPVSGKGISVSVEHLNIALVVYIFAVAMV
                     AISAMVLPGISGSTMLLIFGLYVPVITALKEVMHLHLSYFPVIVIFGLGILTGIALVI
                     KLIRRALENHRSAMIYFVIGMMIGSLYAIVMGPTTLENTKAAMTLHTFHPVFFIIGIV
                     IVFGLEKIKAIAE"
     gene            4390..5070
                     /locus_tag="PLC71_05165"
     CDS             4390..5070
                     /locus_tag="PLC71_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM02063.1"
                     /translation="MNELKILVVDDESRMRKLVKDFLTRAGYQVLEAQDGEEAVDTFY
                     STKGINLLVLDVMMPKMDGWEVVKEIRKVSKVPIIMLTAKDQEQDELLGFELGVDEYI
                     SKPFSPKILVARVEAILRRTVQTEGEQIEAGGIVMDVGGHHVSIDGKSINLSYKEFEL
                     LQYFMSNQGRALTRESILNQVWNYDYFGDARTIDTHVKKLRNKLGEKGSYIHTIWGMG
                     YKFEVEEN"
     gene            5067..6551
                     /locus_tag="PLC71_05170"
     CDS             5067..6551
                     /locus_tag="PLC71_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM02064.1"
                     /translation="MRKKLLKNRSVRVKMTLMLIIIMTSVILLSVLINLTMLKTYYTV
                     KEKKTIKESYYQIQKVFQKDTVRKNDVDEIAKTYNYRVLISDGSGTIVYSSEGEKSLM
                     YKSLWNILNENLDKISNQIKKQGYAVMTNTNKSMGTSINLMGYLDGDYTVIISTPMES
                     IQTSARLSGQFTMYVGLILIICGAVAMYLYSRQFTKPIEDMAKAANQMSHLDFDVKVP
                     TGSDDELGRLGASINELSSKLEFTISELKTANNELQKDIEQKVQIDEMRKEFLSHVSH
                     ELKTPIALIQGYSEGLKDNILDDEESKEFYCDVIADEAKKMNRMVQKLLTLNQIEFGN
                     NQVNMERFDITEMIRNMVESSKILVEKQEVKIIFDEPETHVWADEFMIEEVVNNYLSN
                     ARNHVTDGGIIKVSYCRHGNDVRIKVFNTGDHIPQEDIDKLWVKFYKVDKARTREYGG
                     SGIGLSIVEATMKAHGKDYGVANVEGGVEFYFDVESADSESVQC"
BASE COUNT         2398 a         1007 c         1537 g         1794 t
ORIGIN      
        1 taccgatcag tgcgatcgca gagagacaga atatttcagt cagatactta gagcaattat
       61 tacccaaact gaagaaagcg gggatcatca aaagtattcg tggagcacag ggtggatata
      121 tgttagataa agatccaaaa gatatttcgg caggagatat cttaaggact ttggaaggag
      181 atttaacacc gattgactgt gcagcattta ccggagatac atcgacttgc agcggcacaa
      241 aattctgcgt gaccaagaca gtttggaaac gaatcaatga cagcgttcag caggcggttg
      301 attcgatcac tttacaagaa ttgatggaag aatcaatcga aatacatacc aaatatgatc
      361 aagaagaaag gtaggaatca taatcatgga aaagaaaatc atttacttag atcatgctgc
      421 tactacagca acaagaccgg aagttgtaga tgcaatgctt ccttatttta cagaaaactt
      481 ctggaatcca tccagtgtat atacaccagc attaaataac cgtaaagttg tagacgaatc
      541 aagagaaacg atcgcaagat cacttggaac aacaccagag aatatctact tcacagcagg
      601 tggttctgaa tctgataact gggcattaaa atgtacagca gaagcttatg caaacaaagg
      661 aaaacatatc atcacaagta agatcgaaca tcatgcgatc cttcatacat gtgaatatct
      721 tgagaaaaaa ggatttgaga tcacatatat tgacgttgat gagaacggaa tcttaaaatt
      781 agatgactta aaagcagcga tcagaccaga tacgatctta atctctgtga tgtttgcaaa
      841 caacgagatc ggaacgatcc agccaattaa agaaatcgga gctatcgcaa aagaacatgg
      901 aatcttattc catacagatg cagttcaggc atatgctcag attccaatca atgtagatga
      961 atataatatc gatatgttaa gtgtcagcgg acacaaattc ggtggaccaa agggaatcgg
     1021 attcttatat atcagaaaag gattaaaact tcgttctttc attcatggtg gagcacagga
     1081 aagaaaaaga cgtgctggaa cagagaatat cacaggtatc gtaggaattg caaaagcagt
     1141 tgaaatcgca tttgcaacaa tggatgaaag aatcaagcat gagacagaag tccgtgacta
     1201 tgcgatcaag agaatcctgg cagaagttcc atactgccgt ttaaatggag atgcacaaaa
     1261 gagagtacca aacaacatta atatcagttt ccagttcatc gaaggagaat ctttactgat
     1321 catgcttgat atggcaggaa tctgtgcatc cagtggatca gcatgtacat caggatcctt
     1381 agatccatct catgtactgt tagctattgg actgcctcat gagatcgctc atggatcact
     1441 tcgtatgacg atcggagaag aaacaacaaa agaagatatg gattatgttg tagacaatat
     1501 caaagagatc gtgacaaaat taagaacaat gtcaccatta tatgaagatt ttgtaaagaa
     1561 aaataattaa gaggttttcc atagaggaaa ataatgaaaa taaaatttca gtagaattta
     1621 tagataagat caggaggaat cataatgtac agtgagaaag taatggatca ttttcagcat
     1681 ccaagaaacg taggagaaat tgaaaatgcc agtggtgtgg gaacagttgg taacgcaaaa
     1741 tgtggagata tcatgagaat ctatttagat atcgatgata atggaatcat ccaggatgtt
     1801 aaattcaaga catttggatg tggagcagcc gttgcaacaa gcagtatggc aacagagctt
     1861 gtcaaaggaa agaacattca gcaggcatta gaagtaacaa acaaagcggt tatggaagca
     1921 ttagatggac ttccaccagt gaaagttcac tgttctttac ttgcagaaga agcaattcac
     1981 gcagcattat gggattatgc tgagaaaaat ggtatcaaga tcgaaggatt agaaaaacct
     2041 aaaacagata tcagtgaagg tgaagaagaa gaggaatact aggaggcagt tgtgaaagaa
     2101 aaagtcgtgg ttggaatgtc aggaggcgtt gattcatccg ttgcggcata tctcttaaag
     2161 aaacagggat acgatgtgat cggtgtgaca atgcagatct ggcaggaaga agatgagttt
     2221 gttcaggaag agaatggtgg atgctgtgga ctatcagctg ttgatgatgc aaggcgtgta
     2281 gcacagatga tcggaattcc tcattatgtc atgaatttta gaaaagactt caaagataaa
     2341 gtcattgact atttctgtga agaatattta aatgcaagaa ctccaaatcc atgtatcgca
     2401 tgcaaccgtt atgtgaaatg ggagtcctta ttaaagcgtt gcttagacat tggagcagac
     2461 tatattgcaa ccggacatta ttcacagatc acaaagcttg ataacggacg ttatgcatta
     2521 aaatgttcta agacacagaa gaaagatcag acctatgcat tatataatct gactcagaat
     2581 cagctttcta aaacactcat gccgatcggg gcatacacaa aagatgaagt cagacagatc
     2641 gcagaagaga tcggactgtt agttgccaac aagcctgaca gtcaggatat ctgctttgta
     2701 gatgataact atggcgaatt tatcgaagag acgactggaa caaagattcc aaaagggaac
     2761 tttgtagatc cagaaggcaa tgtcttagga gagcataaag ggatcattta ctatacagta
     2821 ggacagagaa gaggtcttgg attatccttg aagaaaccag gatatgtcat caaagtagac
     2881 aaagatacga atgaagttgt agtcggagac aaacaggatc tgtttgccaa tgttttatat
     2941 ggaaataaag taaactgtat gtctgtacca gagtttgaag atggtatgaa actgatggcg
     3001 aagattcgat acaatagtcc ggaagtccct tgcagagtct atatgacagg ggaagatgaa
     3061 gtaagagttg tctttgatga accacagaga gcgatcacgc caggacaggc agtcgtattc
     3121 tatgatggag acattgttgc aggaggggct acgatctttc gtgcagcaaa tgaaaatatg
     3181 tgaattttat ttcatacttt cttctaaaaa aaagttatgt tacaatacaa tgtgttgtat
     3241 attaaacaca taacaggaga aagaggtagt atgctaaatt ttatcagagg attttgtatg
     3301 gcattagctg acagtgttcc gggtgtatct ggtggaacgg ttgcgttctt acttggtttt
     3361 tatgacaagt ttatcatgtc acttgatcat ttgatgtcag gaacaaaaga agaaagaatt
     3421 gaagcaatta agttcttaat aaggatcggc attggatggg tcgttggaat gggagcagca
     3481 gtttctgttt tggcaagtat ttttgatgac aggatttatg agatcagttc tttattctta
     3541 ggacttatca tttttgcaat tccgatgatc tgcaaagaag agaaagattc attaaaagga
     3601 aaataccaga atatcatctg ggcagtgatt ggagttgtac ttgttgcagc aatcacatac
     3661 tttaatccag tatctggaaa aggaatcagt gttagcgtgg aacatttaaa tatcgcattg
     3721 gttgtttata tcttcgcagt agcgatggta gcaatctcag caatggtact tccaggaatt
     3781 tcaggatcaa caatgcttct gatctttggg ttatatgttc cagtgatcac agcactcaaa
     3841 gaagtgatgc atcttcattt gtcttacttc ccagtcatag tgatctttgg acttggaatc
     3901 ttgacaggaa tcgcactcgt gatcaaactg atccgccgtg cattggagaa ccatagaagt
     3961 gcgatgatct attttgttat cggaatgatg atcggttctt tatatgcgat cgttatggga
     4021 ccaacaacac tggagaatac aaaagcagcg atgacacttc atacgttcca tccagtattc
     4081 tttattatcg gtattgtgat tgtcttcggt cttgagaaga tcaaagcgat tgcagaataa
     4141 tacgaacaat taaatgatac aaaagtatca tgatcataaa agaagttttg cagaaattta
     4201 tctgtgagac ttcttttttt gatatatttt gtgaagagat gatgatcgta aattcatgat
     4261 aaacattcac agttttgaca taatcctatg atagaataaa gtcaacaaaa taagaaggat
     4321 gtttcttttt tataggaaag aaatataaat aaatggtatg agaaaataag attaggagga
     4381 agatacaagg tgaatgaatt aaagatatta gttgtggatg atgaaagcag aatgcgaaag
     4441 ctagtcaaag actttctgac aagagccggc tatcaggtgt tagaagcaca ggatggtgaa
     4501 gaagcggttg atacctttta ttctacaaag ggaatcaatc tgttagtatt agatgttatg
     4561 atgccgaaga tggacggatg ggaagttgtc aaagaaatcc gtaaagtatc taaagttccg
     4621 atcatcatgc tgacggcaaa agatcaggaa caagatgaac ttcttggatt tgaacttgga
     4681 gtggatgaat atatttccaa accattcagc ccaaagatct tggttgccag agtggaagcc
     4741 attttaagaa gaacggttca gacagaaggt gaacagatag aagctggcgg aattgtcatg
     4801 gatgttgggg gacatcatgt ctctattgac ggcaaaagca tcaatctgag ttacaaagaa
     4861 tttgaattat tacagtattt tatgtccaat caaggaagag cccttacaag ggaaagtatc
     4921 ttaaatcagg tatggaacta tgattacttt ggagatgcaa gaacgatcga cacccacgta
     4981 aagaaattaa gaaataaact tggtgaaaaa ggttcttata ttcatacgat ctggggaatg
     5041 ggatacaaat ttgaggttga agagaattga gaaagaaatt attaaagaac cgttcagtca
     5101 gagtcaagat gacattgatg ctgatcatca tcatgacgtc ggtgatctta ttatcagtac
     5161 tgatcaatct tactatgttg aagacatatt atactgtgaa ggaaaagaag acgatcaaag
     5221 aatcctatta ccagatacag aaagtttttc aaaaagatac ggtacggaag aatgatgtgg
     5281 atgagatcgc gaagacttat aattacaggg ttttgatcag tgatggatca ggaacgatcg
     5341 tttatagttc tgaaggagag aagagcttga tgtataaaag cctttggaac attttaaatg
     5401 aaaatcttga taagatcagt aaccagatca agaagcaagg ttatgcagtt atgaccaata
     5461 ccaataagag tatgggaaca agcatcaatc taatgggtta tctggatggt gattatacag
     5521 tgatcataag tacaccgatg gaaagtatcc agacaagtgc cagattatcc ggacagttta
     5581 cgatgtatgt tggactgatc ttgatcattt gcggtgcagt tgcgatgtat ttatacagcc
     5641 gccagtttac aaaaccaatt gaagacatgg ccaaggccgc gaatcagatg tcacatttag
     5701 attttgatgt gaaagtgcca acaggatctg atgatgaatt aggacgactt ggagccagta
     5761 tcaatgagtt atcatctaaa ttggaattca ccatttcaga actgaaaaca gcgaacaatg
     5821 aactgcaaaa agatatcgag cagaaagttc agattgatga gatgcgaaag gaattcttat
     5881 cccatgtatc ccatgaatta aagacaccga tcgcactgat ccagggatat tcggaaggat
     5941 tgaaagacaa tatcttagat gatgaagaaa gcaaagagtt ctattgtgat gttattgcag
     6001 atgaagcaaa gaagatgaac cgtatggtac agaaacttct gacactgaat cagattgaat
     6061 tcggaaataa tcaggtcaat atggaacgat ttgatatcac agaaatgatc cgcaatatgg
     6121 tagaatcaag caagattctt gtggaaaagc aagaagtcaa gatcatattt gatgagccag
     6181 agactcatgt gtgggcagat gaatttatga tcgaagaagt tgtgaacaac tatctgagca
     6241 atgccagaaa tcacgtaaca gatggtggga tcatcaaagt cagctattgt cgtcatggaa
     6301 atgacgtaag gatcaaagta tttaatacgg gagaccatat tccacaggaa gatattgata
     6361 aattgtgggt aaaattctat aaagtcgata aagcaagaac aagagaatac ggtggaagcg
     6421 gaatcggatt gtctatcgta gaagcaacga tgaaagcaca tggcaaagat tatggagttg
     6481 ccaatgtaga aggtggtgta gaattttact ttgatgtgga atctgcggat tctgaatctg
     6541 tacaatgtta aaaaagcatt ttcattgtgt tgtgtatgga agtaaaaatg ctacagacaa
     6601 agaaatgagt tttgtggtat gatagataaa tagtgtaagc aaaaagaaat cagtgtaaac
     6661 tggtttcttt tggataatag gataaaaagg atgaaaagaa atatgttaaa agattacaaa
     6721 gaacagattc aggatg
//
LOCUS       DAOMUE010000085         6723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3039_length_6723_cov_2.052639, whole genome shotgun sequence.
ACCESSION   DAOMUE010000085 DAOMUE010000000
VERSION     DAOMUE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6723
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3039_length_6723_cov_2.052639"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLC71_05175"
     CDS             <1..375
                     /locus_tag="PLC71_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM02065.1"
                     /translation="PFKGAMITNGRINFSEMIDNILVFCPFGIYIGVLYRRWNLSEKV
                     LSFFSVSLIYEVLQYVLAIGRSDITDVIMNTVGGIVGLLIYSLLNKICRNERMTEIVV
                     FICAAFGSAAVSGFLLAIFHAN"
     gene            complement(755..2050)
                     /locus_tag="PLC71_05180"
     CDS             complement(755..2050)
                     /locus_tag="PLC71_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HRM02066.1"
                     /translation="MSKERNYKFETLQLHVGQEQPDPVTDARAVPIYQTSSYVFRNCD
                     HAAARFGLADAGNIYGRLTNPTEDVFEQRIAALEGGVAALAVASGAAAITYAIENITK
                     AGDHVVAAKNIYGGTYNFLEHTLPDYGIETTFVDIFNEDEVRGAIQDNTKLLFIETLG
                     NPNSDVVDIEKAAAIAHEHNIPLIVDNTFATPYLVRPIEYGADIVVHSATKFIGGHGT
                     TIGGVIIDGGKFDWEASGKFPSLVEPNPSYHGVSFTKDVGAAAFVTKIRALLLRDTGA
                     TLSPVHAFIFLQGLETLSLRVERHVQNALKVVEYLKNHPAVEKVNHPSVSDDPEQQEL
                     YKKYFPNGGGSIFTFEIKGDAQKAKDFIDHLELFSLLANVADVKSLVIHPASTTHSQM
                     TEEELLGSGIKPNTIRLSIGTENIDDIIEDLEEAFKAVQ"
     gene            complement(2167..3099)
                     /locus_tag="PLC71_05185"
     CDS             complement(2167..3099)
                     /locus_tag="PLC71_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM02067.1"
                     /translation="MTLTQLRYIIAIVDTGSMNEAAKSLFISQPSLSLAVKELEKEIG
                     TRLFKRSNRGVSVTQEGMEFLSYARQVVEQYQLMESRYIEKKNIKKKFGVSMQHYTFA
                     VNAFVEMVKQFGMDEYEFAVRETKTYEVIKDVKDFHSEIGILYLNEFNKKVLSKMLQE
                     SGLEFHPLLECGIYVYMWKGHPLADKEDITIEELEEYPCLSFEQGEYNSFYFAEEVLS
                     TYEYKRLIKANDRATMLNLMVGLNGYTLCSGIICESLNGDDYCAVKLKSDELMTIGYI
                     KRKGVAISPLGEKYLEEIKKYKAMGDDSGYRDIL"
     gene            3229..3717
                     /locus_tag="PLC71_05190"
     CDS             3229..3717
                     /locus_tag="PLC71_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM02068.1"
                     /translation="MAVSIVRILTISFLIIKGASSMYESSYHYLLMSNHLSLQKKIMA
                     VLKPLGLSTGQPKVLDHLRYHNGASQKDIAHACHIEPASLTSILNRMEKQHMIERKNL
                     NGNRRSFHIFLTDYGMELSESIEKAFLSLEHEVFSGISNAEKEQFLKIFEKLYKNTTK
                     EE"
     gene            3726..5003
                     /locus_tag="PLC71_05195"
     CDS             3726..5003
                     /locus_tag="PLC71_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HRM02069.1"
                     /translation="MEKLKRYIIFFIGLYINSLGVALITKASLGTSPISSIPYVLSLN
                     FPFTLGNFTIFFSLLLIFLQLLILRKNFKLEHVLQIPISILFGYFIDWSMILLGFVNP
                     SFYLMKIIYLLIGCLILGFGVYTEVLANVAMLPGESFVRAIVMTWHTEFGVTKICFDV
                     SMTVIAGVLSFVFAGKLDGVREGTVIAALLVGFIARLFGRFLSFVEPMLFPDSDTNTD
                     SDTDTDSTDTPIVIAIAREYGSGGHDLGEALAKRLGFDFYDNEIIQMAAGSTGYDPEF
                     IKNNDEQMRSSLLYDLVNQVYSHAPQNPAPKDNIFAAESKVINELASKGNCVVIGRCA
                     DYILRDRPHCMRIFLHAPAEYRVKRIMRTENLSHDAALTKAIQMNRKRSDNYHYYTHQ
                     PWGLSRNYHLTLDTSLGDDFVLDSICEGLKHIQ"
     gene            complement(5116..6678)
                     /locus_tag="PLC71_05200"
     CDS             complement(5116..6678)
                     /locus_tag="PLC71_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM02070.1"
                     /translation="MSKTFNVNGICYPNEHYMVNIDSKLAQIKQLVDEKKYFVINRAR
                     QYGKTTTLNMLVKYLADKYTVFFISFEGLGETAFESETAFCGTICELLYDTVLYEEVP
                     TIDDTVKQIIIDSIKKKAQDMLSLSSLISNICKNAEVPVILMIDEVDQASDHKVFIDF
                     LGMLRSKFLKRSTRPTFQSVILAGVYDIKNLKHRIREDHEHQMNSPWNIAADFSVDMS
                     FTRDDIASMLDEYEKDYKCTMEVKKIAELIYEYTSGYPYLVSKICKLIDERCDQNWTK
                     QGVLEAIKILLKETNTLFDDLRKKITDYKELKNMLYSILFQGESYPYNPDNFVIDIGT
                     MFGFIKENDGQVVISNRIFETRLYNLFLSEELTSSVIYQTGERDKNQFVKNGILNMEL
                     VLEKFMIHFHDIYGKNTEKFVEENGRRVFLLYLKPIINGTGNYYIEAQTRNQSRTDVI
                     VDYLGKQYVIELKIWRGNEYNKRGEKQLAEYLEYYHLEKGYLLSFNFNKNKKIGLKEV
                     EVDNKKIIEVVV"
BASE COUNT         1850 a         1318 c         1068 g         2487 t
ORIGIN      
        1 ccattcaaag gtgccatgat cacaaatgga cgaatcaact tttctgagat gatcgataat
       61 attcttgttt tctgcccttt tggaatttat attggagttt tgtatcgacg ttggaatttg
      121 tctgagaaag tgttgtcttt cttctctgta agcttgattt atgaggttct gcagtatgtt
      181 ttggcaattg gacgcagtga tattacggat gtaatcatga atacagttgg gggaattgtt
      241 ggacttttga tttattctct gcttaataag atctgtcgaa atgaacgtat gacagaaatt
      301 gttgtattca tctgcgctgc ttttggaagt gcggctgtat ctggattttt attggcaatt
      361 tttcacgcta attaaatttg aatttagtct ttgatgtatg tgatggaaat aaaggctatc
      421 ggaattcccg taggttgttt cctgttatag gttaaaggat tccaatttaa gtaatatacg
      481 acaacagttt tttccttaca aatttctgtt tgtctggaac taaattaata ttctagaacc
      541 tattatattt catcatctct ctgctatcaa tatgttttat catatactca tagcctacaa
      601 ttggccaatc aaattaaatt ttacaaaaaa tgcccgaaca agctatccta cacttatccg
      661 ggcattttcg ttttaaggtc gagattatat tattattttt taggaaaatt gacatttatg
      721 ttttagtttt tatgtttcgc aagaaaaatc tagcctactg tactgcttta aaagcttcct
      781 ctaaatcttc gatgatatca tcgatatttt ctgtaccaat tgacagacga attgtgttag
      841 gtttgattcc tgatcctaat aattcttctt cagtcatctg agaatgtgtt gtagatgctg
      901 gatggatgac taaggatttc acgtctgcta cgtttgcaag cagtgagaat aactctaagt
      961 gatcgataaa gtcttttgct ttctgtgcat cacctttgat ttcgaatgtg aagatagatc
     1021 caccaccgtt agggaagtat tttttgtata attcctgctg ctcaggatcg tcagatactg
     1081 atggatggtt tactttttct actgctggat ggtttttcag gtattctaca acttttaatg
     1141 cattctgtac atgacgctct actcttaagg ataatgtttc taatccctgt aagaagatga
     1201 atgcatgaac tggagataat gttgctcctg tgtcacgaag taataatgct ctgatctttg
     1261 ttacgaatgc agctgctcca acgtcttttg taaagcttac tccatggtag cttgggtttg
     1321 gttctactaa tgatgggaat tttcctgatg cttcccagtc gaatttacca ccgtcaatga
     1381 tgacaccacc gattgttgtt ccgtgaccac cgatgaattt tgttgctgaa tgtactacga
     1441 tgtctgctcc gtattcgatt ggtcttacaa ggtatggtgt tgcaaatgta ttatctacga
     1501 tcagtggaat gttgtgttca tgagcgatcg ctgctgcttt ctcaatgtct acaacgtctg
     1561 agtttggatt tcctaatgtt tcgatgaata ataattttgt gttatcttga attgctcctc
     1621 ttacttcatc ttcattgaaa atgtctacga atgttgtttc gattccgtaa tctggtaatg
     1681 tatgctctaa gaagttgtat gttccaccgt aaatattttt cgctgcaact acgtgatctc
     1741 ctgcttttgt aatattttca atcgcatatg tgatcgctgc tgctccagat gctactgcta
     1801 atgctgcaac tccaccttct agtgctgcga ttctctgttc aaaaacatct tctgttgggt
     1861 ttgttaatct tccgtagatg ttacctgcat ctgcaagtcc gaaacgagct gctgcatgat
     1921 cgcaatttct gaatacataa gatgatgtct gataaatagg tactgctctt gcatctgtca
     1981 ctggatctgg ctgttcctgt cctacgtgta actgtaatgt ttcaaactta taatttcttt
     2041 ctttactcat aatttctatc tcctttgttg acttttattt tgaattttgt atgttgtctt
     2101 tcttgattac aaacttatct taccattaga tttcctactt gtccaatatg aaaagtcaac
     2161 atctagttat aggatatccc tataaccaga gtcgtcaccc atagctttat actttttgat
     2221 ctcctctaag tacttctctc ctaatgggct gatcgcgact ccttttcgct tgatatagcc
     2281 aatcgtcatg agctcatccg attttagttt cacagcacag taatcatcac catttaagct
     2341 ttcacagata attcctgaac ataatgtata tccatttaac ccaaccatga gatttaacat
     2401 agtagctctg tcatttgctt tgatcagacg tttatattca taggtactta acacttcttc
     2461 tgcaaaataa aaggagttat attccccctg ttcaaatgac aggcatggat attcttccaa
     2521 ctcttcgatc gtaatgtctt ctttatctgc cagaggatgc cctttccaca tataaacgta
     2581 aattccacat tccaataatg gatggaattc aagccctgat tcctgcagca tcttgcttaa
     2641 taccttctta ttaaattcat ttaaataaag aatcccgatc tcactgtgaa aatctttgac
     2701 atccttgatc acttcgtatg tctttgtctc tctgactgca aattcgtatt catccatacc
     2761 aaactgtttg accatctcca caaaagcatt cactgcaaat gtataatgct gcatggaaac
     2821 tccgaatttt ttcttgatat ttttcttctc aatatagcga gattccataa gctgatactg
     2881 ttcaacgacc tgtcttgcat aacttaagaa ttccatccct tcttgtgtga ctgatactcc
     2941 tcgattgctt ctcttaaaga gtcttgttcc aatctctttt tctaattctt tgactgcaag
     3001 agatagactt ggctgtgaga taaacaggct ttttgccgcc tcattcatag atcctgtatc
     3061 tacgatcgct ataatatatc ttaattgtgt taatgtcatg gttcttccct tccttatctt
     3121 ttgaaactct ctaatgtccg tggtttatta tacttgattt ttcgccgctt gtcttatgat
     3181 ttttgaaaga aacgctcttg acatattggt ttgctttgtc ttataataat ggctgtttca
     3241 atagttagaa ttctaactat tagttttcta atcataaaag gagcatcatc tatgtatgaa
     3301 tcatcttatc attatcttct gatgtccaat catttaagcc ttcagaagaa aatcatggct
     3361 gtcttaaagc cacttggtct ctcaaccggc cagccaaagg tcttagacca tcttcgatat
     3421 cataatggtg ccagtcaaaa agacattgcc catgcttgtc atatcgaacc tgcttctttg
     3481 acttctattt taaatcgaat ggaaaaacag catatgatcg agcgtaagaa tttgaacgga
     3541 aatcgccgat cctttcatat tttcctgaca gattatggaa tggaactgtc tgaatcgatt
     3601 gaaaaagctt tcttatctct tgaacacgaa gttttttctg ggatttctaa tgcagaaaaa
     3661 gaacaatttc taaagatctt tgaaaaacta tataaaaata ctacaaaaga ggagtaaatc
     3721 acatcatgga aaaactaaaa cgttatatca tcttttttat tggactttat attaactctc
     3781 tcggagttgc cctgatcaca aaagcaagtc ttggtacatc accgatttct tccattcctt
     3841 atgtactaag ccttaatttc ccatttacac ttggaaattt caccattttc ttcagcctgt
     3901 tactgatctt tttacagctg ttgatcttac gaaagaattt taaactggaa cacgtgttac
     3961 agattccaat ctctatctta ttcggatatt ttattgactg gtctatgatc cttcttggat
     4021 ttgtaaatcc gtccttttat ttgatgaaga tcatttatct gctgatcgga tgtctgatcc
     4081 ttggatttgg tgtgtataca gaagtccttg ccaatgttgc catgcttcct ggggaatctt
     4141 ttgtacgtgc cattgtaatg acatggcata cagaatttgg tgtcacaaaa atctgttttg
     4201 acgtatctat gactgtgatt gccggagttc tttcctttgt attcgcaggt aagttagacg
     4261 gtgtacgtga aggtacagtt attgccgctt tacttgttgg atttattgca cgtttatttg
     4321 gacgtttctt atcttttgtg gagccaatgc tgttcccaga ttcagacact aacacagatt
     4381 cagacactga cacagattca acagacactc ctattgtcat tgcgatcgct cgtgaatatg
     4441 gaagcggtgg acatgacctt ggagaagctc ttgcaaaacg tcttggattt gatttctatg
     4501 acaatgagat tattcagatg gctgcaggtt ctactggtta tgatcctgaa ttcatcaaga
     4561 ataatgatga acagatgcgt agttccctgc tttatgatct tgtgaatcag gtatattctc
     4621 acgcaccaca gaacccagct ccaaaagata atatctttgc ggcagaatct aaggtgatca
     4681 atgagcttgc ttcaaaagga aattgtgttg tgatcggtcg ttgtgcggat tatatcttaa
     4741 gagaccgccc tcattgcatg cgaatcttct tacatgcacc tgccgagtat cgtgtgaaac
     4801 gaattatgag aacggaaaac ttatctcatg atgcggcact gacgaaagca atccagatga
     4861 accgcaagcg ttctgataac tatcattatt atacacatca accatgggga ttatccagaa
     4921 actaccatct gacactggac acttcacttg gagatgattt tgtgcttgat agcatttgtg
     4981 aagggttgaa acatattcag taatttattg tgattgtaaa aaggatagat gccttggttt
     5041 ttggcagcta tccttttcta tttcctgttc taactcctct gaaatttgct tatctgagaa
     5101 cccttaactg tatttttaca ctactacttc tataatcttc ttattatcta cttccacttc
     5161 ttttaatcct atttttttat ttttattgaa gttaaaactc agcaaatatc ccttttctaa
     5221 atggtaatat tctaagtatt ctgctaactg cttttctcct cttttgttat actcattacc
     5281 tctccagatt ttcaattcaa tcacatactg tttccccaaa tagtccacaa taacatctgt
     5341 tctactctga tttcttgtct gtgcttctat ataataattt ccagtaccat taataatcgg
     5401 ttttaaatat agcaaaaata cacgccggcc attttcttct acaaattttt ctgtattttt
     5461 cccataaata tcatgaaaat gtatcataaa cttttcaagc accaattcca tatttaatat
     5521 accgtttttc acaaattgat tcttatcacg ttctcctgtt tgatatatca cactacttgt
     5581 taattcttcc gacaaaaaca aattgtataa tctggtctca aatatacgat ttgaaataac
     5641 tacctgacca tcgttttcct taataaatcc aaacatcgtt ccaatatcta tcacaaagtt
     5701 atctggatta tatggatagg attctccttg aaataatatg gaatatagca tatttttcaa
     5761 ttctttataa tctgtgatct tctttctcag atcatcaaac aatgtattag tctcttttaa
     5821 aagaatcttg attgcttcta atactccttg ttttgtccaa ttttgatcgc atctctcatc
     5881 gattaacttg cagatctttg ataccaagta tggataacct gaagtatatt cataaatcaa
     5941 ttccgcaatc tttttcactt ccatcgtgca tttataatct ttctcatatt catcaagcat
     6001 acttgcaata tcatctcttg taaaactcat atctacacta aaatctgcgg ctatattcca
     6061 cggactattc atctgatgtt catggtcttc tcttatacga tgctttaaat tttttatgtc
     6121 ataaacccca gctaatatga ctgattgaaa cgttggcctt gtactgcgtt ttagaaattt
     6181 gcttcgaagc attcctagaa aatctataaa aactttatga tcactcgcct gatcaacctc
     6241 atcaatcatt aaaattacag gaacttctgc atttttgcaa atattggata tcaaactaga
     6301 aaggcttaac atatcttgtg ctttcttctt tatactgtca atgataatct gttttactgt
     6361 atcatcaatt gtaggaactt cttcatatag taccgtatca tacaataatt cacatatagt
     6421 tccacaaaat gctgtttcgc tttcaaaagc tgtttctcca agaccttcga aactaataaa
     6481 aaatactgta tatttatctg cgagatattt gactaacata tttaatgtag ttgtctttcc
     6541 atattgtctc gcccgattta ttacaaaata ttttttctca tccaccaact gctttatttg
     6601 tgctaacttt gaatctatgt ttaccatata atgctcattc ggataacaaa ttccatttac
     6661 attaaatgtt ttactcatat ccctcaccaa ctttctttta tatatagcat tttcttacta
     6721 tgt
//
LOCUS       DAOMUE010000086         6704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3052_length_6704_cov_2.231012, whole genome shotgun sequence.
ACCESSION   DAOMUE010000086 DAOMUE010000000
VERSION     DAOMUE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3052_length_6704_cov_2.231012"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLC71_05205"
     CDS             <1..635
                     /locus_tag="PLC71_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRM02071.1"
                     /translation="TGRTQKKIDMAARLYTVEEAKKNGVDLVYVNTRDIADVNKELRK
                     YSEKGFDDVFIFAPNEEMVTYGVKMLAFDGCLNFFSGPADQEFSSRVNFYNIHYNSTH
                     FVGTSGGNTEDMKQSIELIENKTVNVAKIATHILGLDHAVETTKALPELEGSKKIVYT
                     HKKFPLTEVDKFDENSDDEYIRELKSIVERNGNLWSGEAERYFIQNAPEI"
     gene            647..1495
                     /locus_tag="PLC71_05210"
     CDS             647..1495
                     /locus_tag="PLC71_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM02072.1"
                     /translation="MSSDILVRDQIFSTYDSLYDAEKKVADYLISHPSEAIEMSVSEL
                     ASHCDASQATIIRFCKKIGCKGFHQLKIKLAGEQRQQEEQVISNEIDIDQMDKSLNNI
                     MISKVEEIKATFHNFNPEELREIVDLIIHANLIEFAAMGDTIPIALDGSYKFNQLGLK
                     AVSSTIWESQEAFSRTLRKGDVFFAISASGASKRLVKMVEIVKDNGVTTIAITNQSKS
                     PLAELCDHVLLTATREHIFHDQVSFTRMAAMSVIDTLFLLLFSTKWDSFENVAQHEQS
                     VAEEKL"
     gene            1511..2320
                     /locus_tag="PLC71_05215"
     CDS             1511..2320
                     /locus_tag="PLC71_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003370065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM02073.1"
                     /translation="MNNDWLELEGKTVIVTGAASGIGKEVAQEFLHIGANVVISDMAE
                     NAPEMDAKEGQLLYVRTDVTKVSDVEAMVAKTIETFGTIDVLVNNAGINIPRLLVDPK
                     DPKGQYELDEAVWDKVCNVNLKGVFFCAQAVGRILVEEGVIINMSSESGLEGSEGQSV
                     YAATKNAVNSLTRSWAKELGKQGVRVVGVAPGIMEATGLRTIGYETALAYTRGITVEQ
                     LRAGYSKTSTIPLGRSGKLSEVADLCVYLASKRSSYVHGVTVNVAGGKTRG"
     gene            2710..3009
                     /locus_tag="PLC71_05220"
     CDS             2710..3009
                     /locus_tag="PLC71_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02074.1"
                     /translation="MISMGYAQTNAFTEPVTLMLAVSKDDHRVVDAWEVTQEEMENSC
                     RSCEEYIGMDLIKYYKENQERIEKSQMMNQTVGRDSAKKQAFDMAVQQILGKLGK"
     gene            3027..4265
                     /gene="hflX"
                     /locus_tag="PLC71_05225"
     CDS             3027..4265
                     /gene="hflX"
                     /locus_tag="PLC71_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRM02075.1"
                     /translation="MEEIKKEIIEKVILVAVADQDTTEAEESLDELEELVKTAGAEVA
                     ARVIQVRETPHPGTYIGKGKIDEVNALLYGTDATGIVCDDELSPAQISNLEEALDTKV
                     MDRTLIILDIFAKRAFTREGKIQVELAQLKYRASKLTGQGRALSRLGGGIGTRGPGEK
                     KLEMDRRLIRTRISRLKAELRDVVKHREVQRKQRQKNHLPVVCIVGYTNAGKSTLLNH
                     FTNAGVYEEDQLFATLDPTTKSLDLSGGQTILMTDTVGFIRKLPHHLVEAFKSTLEEA
                     KYSDLILHVVDASNPQKEKQMEAVYDTLKQLGANESPIITAFNKIDLLNGDEILKDPN
                     AEAVVRISGKNGEGTDQLLEQIEKILQKQKLYLEKLYGYQEAGKIQLIRSHGQLLKEE
                     YRDDGIYVEAYIPKEILGSI"
     gene            4277..4774
                     /locus_tag="PLC71_05230"
     CDS             4277..4774
                     /locus_tag="PLC71_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
                     /protein_id="HRM02076.1"
                     /translation="MIIQKVINNNVVSTFDSNGKEVILMGKGIGFRKKTGDELDKTKI
                     EKIFTLDLSGKSSFVQEVMAIIQEFYEIVLDTEHPAYQRFATHLQYLETEILSKKRPI
                     EEDEEDDVEFYERNQKKYPEAYVCSQKIVEHIEQHYNCKLSADEKMYLIIYVKRLILE
                     MGHQS"
     gene            4998..5261
                     /locus_tag="PLC71_05235"
     CDS             4998..5261
                     /locus_tag="PLC71_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRM02077.1"
                     /translation="MVSKKFVIENEQGLHMRPAGVLAKAVTKFESDVTIVFEDKKINA
                     KSLLNIIGACIKCGSEIELVCEGPDEEAALAHATELIESGLGE"
     gene            5314..>6704
                     /gene="ptsP"
                     /locus_tag="PLC71_05240"
     CDS             5314..>6704
                     /gene="ptsP"
                     /locus_tag="PLC71_05240"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRM02078.1"
                     /translation="MYKGIGASAGIGIGKIVKIKEEELNYTKQSIEDTEAEKKRLSDA
                     IEVFIEKTQKMVESMKVTAGEQEAEILEGHIMMIQDPAISEQIEAKIDGEKINAEAAV
                     EEACDFFAQIFAMADDELTQQRASDLGDIKTRLIKILLGIEEVDISAVPEGTILVAED
                     LTPSMTAGINPANVQGVLTEIGGKTSHSAIICRSMEIPAVLSIENIVSIVNDGDEVVL
                     DGSTGEAFINPEASVVEEYKAKKAKFLEEKAALQKFVGQKSQTADGHVVELVANIGGP
                     DEAEGVLERDGEGVGLFRSEFLFMESDAIPSEEAQFEAYKKVAETLDGKPVIIRTLDI
                     GGDKALPYLGLPTEENPFLGFRAVRFCLQRKEDIYKPQLRALLRASAFGKVRIMVPLV
                     TCVDELRAVKAIIEELKQELDAEGIAYDKDIQVGVMMETAAASLIADILAKEADFFSI
                     GTNDLTQYTLAIDR"
BASE COUNT         2388 a         1054 c         1567 g         1695 t
ORIGIN      
        1 tcacaggaag aacacagaag aagattgata tggcagcaag gttatacaca gtagaagaag
       61 caaagaagaa tggtgtagat cttgtatacg tcaatacaag agatatagca gatgtcaata
      121 aagagctacg taaatattca gaaaaaggat ttgatgatgt ctttatcttt gcaccaaatg
      181 aagaaatggt aacatacgga gttaaaatgt tagcattcga tggatgtttg aatttcttct
      241 cagggcctgc cgatcaagaa ttttcatcca gagtaaattt ctataatatt cattataatt
      301 ctacacattt tgtgggaaca agtggtggaa atacggaaga tatgaaacaa tccattgaac
      361 tgatcgagaa caagacagtc aatgttgcaa agatcgcaac acatatatta gggcttgacc
      421 atgcggttga aacaacaaag gcgttaccag aacttgaagg aagtaaaaaa attgtctata
      481 cgcacaaaaa atttccatta acagaggttg acaaattcga tgaaaacagc gatgatgagt
      541 atatcagaga attaaaatca atcgtagaac gcaatggtaa tttatggagc ggtgaggcag
      601 aaaggtattt catacagaat gctcctgaga tttagaaggg agtagtatga gtagtgatat
      661 cttagttaga gaccagattt tttcaacata tgatagttta tatgatgcag agaagaaagt
      721 agccgattat ctgatcagtc atccatctga agcgatcgag atgtctgtat cagaacttgc
      781 atctcactgt gatgcaagcc aggcaacgat catccgtttt tgtaagaaga tcgggtgtaa
      841 gggatttcat cagttgaaga tcaagctggc cggagaacaa cgacagcagg aagaacaggt
      901 gatatcgaac gagatcgata tcgatcagat ggacaaatca ctcaataata tcatgatcag
      961 taaagtggaa gagatcaaag cgacgtttca caattttaat ccagaagaat taagagagat
     1021 cgtggatctg atcatacatg ccaatctgat tgaattcgct gcgatgggag atacgatccc
     1081 gattgcactg gatggatctt acaagttcaa tcagttagga ttgaaagcag ttagttcaac
     1141 aatctgggag agtcaggaag cattttccag aactttgaga aaaggtgatg tattttttgc
     1201 gatttccgcc tctggggcat ccaaaagact tgtgaagatg gtagaaatcg tgaaagataa
     1261 tggtgtaaca acaattgcga tcaccaatca atccaaatcg ccacttgcag agttatgtga
     1321 tcatgttttg ctgacagcaa caagagaaca cattttccat gatcaggtta gtttcacaag
     1381 aatggcagcg atgtcagtga tcgacacact gttcttatta ctgttctcca caaaatggga
     1441 ttcatttgag aatgtggcac aacacgagca gtctgttgca gaagaaaaat tataaaagga
     1501 gatcataacg atgaataacg attggttaga attagaaggg aagacagtca tcgtcacagg
     1561 cgcagcatcc ggaatcggga aggaagttgc gcaggagttt ttgcatattg gggcgaatgt
     1621 agttatttct gatatggcag agaatgcacc agagatggat gcaaaagaag gccagttatt
     1681 atatgtaaga acagatgtaa caaaagtttc tgatgttgaa gctatggtag caaaaacaat
     1741 agaaacattc ggaacaattg atgtactagt gaataatgca ggaatcaata ttccacgtct
     1801 gttagttgat ccaaaggatc caaaaggaca atatgaatta gatgaggcag tatgggataa
     1861 agtatgcaat gtaaatctaa aaggtgtttt cttctgtgcg caggcagttg gaaggatctt
     1921 ggttgaagaa ggtgtcatta tcaacatgtc ttctgaaagt ggtctggaag gatccgaagg
     1981 acagagtgtt tacgcagcta caaagaatgc agtaaactct ttaacaagat catgggcaaa
     2041 agaattagga aaacagggag ttcgcgttgt cggagtagca cctggaatca tggaagctac
     2101 aggacttcgt acgatcggtt atgaaacagc tctggcttat acaagaggga tcacagtaga
     2161 acagttaaga gcaggatatt ccaaaacatc cacaattcca ctaggacgtt ctggaaagct
     2221 ttctgaagtt gcagatctgt gtgtatatct tgcaagcaag agatcttctt acgtacatgg
     2281 tgtgacggtt aacgttgcgg gtggtaagac aagaggatag aataatttct gatagaagat
     2341 atagaaggta aagatatcag ctggttttag gatcagctga tatctttttt taacataata
     2401 tagcaggaaa ttccgatgga actctctatg atataagaaa gtatggcaaa gccggtttct
     2461 ttctggttgc tacaaaaaaa gaatcgttgt ataatgattc ttagcatcac aaattaaaaa
     2521 gaaaggaaat atgatgtttt cgtttctata aaaacatcat acaagaataa aatgttaatt
     2581 ctggctgtag gaatcggaat tgtattttgt ataatagtat ttatttgtta ttcttataaa
     2641 caaaatcaac ggtcaaaata cacatccgaa gtatacaaag tatttaaagg acaatatggg
     2701 agaggatcag tgatctctat gggttatgca cagacgaatg catttacaga acccgtaaca
     2761 ttaatgttgg cagtcagcaa ggatgatcac agagttgtag atgcatggga agttacgcaa
     2821 gaggagatgg agaatagctg tcggtcatgt gaagaatata taggaatgga cctgatcaaa
     2881 tattataaag agaatcagga aagaattgaa aaaagtcaaa tgatgaatca gacggtgggc
     2941 agggattcag cgaagaagca agcatttgac atggcagtcc aacagatatt agggaaatta
     3001 ggaaaataga tacaggaaca tatattatgg aagaaatcaa aaaagaaatc atagagaaag
     3061 tgatcttggt cgcggttgct gatcaagaca caacagaagc agaagaatct ctggatgagt
     3121 tagaagaact ggtcaaaacg gcaggagccg aggttgcagc aagggtgatc caggtaagag
     3181 aaacaccaca tccaggaact tatatcggaa aaggtaagat tgatgaagtc aatgctttgt
     3241 tatacggaac tgatgcgaca gggatcgtct gtgatgacga attatcacca gcacagatca
     3301 gcaatttaga agaagcatta gatactaaag tgatggatcg aacactgatc attcttgata
     3361 tttttgcgaa gcgagcattt acaagagaag gtaagattca ggtcgaactt gcccagttaa
     3421 aatatagagc atccaagctt accggacaag gaagagcctt atcaagatta ggtggaggaa
     3481 tcggaaccag aggtccggga gagaagaaac tggagatgga ccgaagactg atccgtacaa
     3541 gaatctccag attaaaagca gagttaagag atgttgtaaa gcacagagaa gtccagagaa
     3601 aacaacgcca gaagaatcac ctgccagtgg tttgtatcgt tggatatacc aatgcaggaa
     3661 agtcaacact attaaaccat tttaccaatg caggagttta tgaagaagat cagttatttg
     3721 caacccttga tccaacgaca aagagtttag atctaagcgg cgggcagaca attcttatga
     3781 cggatacggt cggttttatc cgcaaacttc cacatcatct ggtcgaagca tttaaaagta
     3841 cattagaaga agccaaatac agtgatctga ttctgcatgt agttgatgca tccaatccgc
     3901 agaaagagaa acagatggaa gcagtctatg atacattaaa gcagttagga gctaacgaat
     3961 caccgatcat tacagcattc aataagattg atctgttaaa tggtgatgag atcttaaaag
     4021 atccaaatgc agaagcggtg gtaaggattt ccgggaaaaa tggcgaagga accgatcagt
     4081 tgttagagca gattgagaaa atactgcaaa aacagaaatt atatttagaa aagctgtatg
     4141 gatatcagga agcaggtaag atccagctga tccgaagtca tggacagctt ttgaaagaag
     4201 aatacagaga tgatggaatt tacgtagaag catacattcc aaaagagatt cttgggagta
     4261 tctaggaggc gagaaagtga tcatacagaa agtaatcaac aataatgtgg tcagtacatt
     4321 tgattcaaac ggaaaagaag ttattttgat gggaaaagga attggatttc gcaagaaaac
     4381 cggggatgaa ttagataaga cgaagattga gaagattttt accttggacc ttagcggcaa
     4441 atcatctttt gtacaagaag tcatggcgat cattcaggag ttttatgaaa tcgtgctaga
     4501 tacggaacat ccagcatatc agagatttgc aacacattta caatatcttg agacggaaat
     4561 cttatcaaag aaacgcccga tagaagaaga cgaagaagat gatgttgaat tttatgaacg
     4621 taatcagaag aagtatccag aagcctatgt gtgcagtcag aagatcgtgg aacatatcga
     4681 gcagcattac aactgcaaac tttcagcaga tgagaagatg tatctgatca tttatgtcaa
     4741 aagactgatc ttagaaatgg ggcatcagtc ataaaaccag gttgacttat aaggcaaaat
     4801 ttgatattat aatattatcg aattttggat tgtgactggt gaggcaggca agacctattt
     4861 aataagtgtg aacatttgtt aaagtaggtc tttttttatg cttttttaag gaattgcact
     4921 tagatgagta caaagataag ccagttaaag atccagataa attaaaatta ttttatatgt
     4981 gagaaggaga aacaattatg gtatcaaaga aatttgtcat tgaaaacgaa cagggattac
     5041 acatgagacc agctggagtt ttagcgaaag ctgtaactaa attcgaatct gacgtaacta
     5101 tcgtattcga agataaaaaa atcaacgcta aaagcttatt aaacatcatc ggagcttgca
     5161 tcaaatgtgg ttctgaaatc gaattagtat gtgaaggtcc agacgaagaa gcagctcttg
     5221 cacatgcaac agaactgatt gaatctggat taggtgaata atcactgctc ataacgggtg
     5281 taacatataa tttgataaag gacgggtggc aacatgtata aaggtatagg agcatctgct
     5341 ggtatcggta tcggtaagat cgttaagatt aaggaagaag aacttaatta cacaaaacag
     5401 tctatcgaag atacagaagc agagaaaaaa cgtttaagcg atgcaattga agttttcatt
     5461 gagaagactc agaagatggt tgaatccatg aaagtaactg ctggtgagca ggaagcagag
     5521 atcttagaag gtcatatcat gatgatccag gatccagcga tctcagaaca gatcgaagct
     5581 aagatcgatg gagagaagat caacgcagaa gctgctgtag aagaagcttg cgatttcttc
     5641 gcacagatct tcgctatggc agacgatgaa ctgacacagc agagagcatc tgacttaggc
     5701 gacatcaaaa ctcgtttaat taagatctta cttggaattg aagaagtaga tatcagtgca
     5761 gttccagaag gaacaatctt agtagcagaa gatttaacac catctatgac agcaggaatc
     5821 aatcctgcaa acgttcaggg tgtattaaca gagatcggtg gtaagacatc tcactctgcg
     5881 atcatctgca gatctatgga aattccagca gttcttagta tcgaaaacat cgtttctatc
     5941 gttaatgatg gagatgaagt tgttcttgac ggatcaacag gagaagcatt catcaatcct
     6001 gaagcatctg ttgtagaaga atataaagct aagaaagcaa aattcttaga agagaaagct
     6061 gcccttcaga agtttgttgg acagaaatcc cagacagcag acggacacgt tgtggaactg
     6121 gttgctaaca tcggtggacc agacgaagca gaaggtgtct tagagcgtga tggagaaggt
     6181 gttggattat tcagatctga gttcttattc atggaaagcg atgcgattcc atcagaagaa
     6241 gctcagtttg aagcatacaa gaaagtagct gaaactcttg acggtaaacc agtgatcatc
     6301 cgtacactgg atatcggtgg agataaagca cttccatatc ttggacttcc aacagaagag
     6361 aatccattct taggattcag agcagtacgt ttctgcttac agagaaaaga agatatttac
     6421 aaaccacaac ttcgtgcatt attacgtgca agtgccttcg gtaaagttcg tatcatggta
     6481 ccattagtaa catgtgtaga cgagcttcgt gcagtcaaag cgatcatcga agaactgaaa
     6541 caggaattag atgcagaagg tatcgcatac gacaaagaca tccaggttgg tgttatgatg
     6601 gaaactgcag cagcaagctt aattgctgac attcttgcaa aagaagcaga cttcttcagt
     6661 atcggtacaa acgacctgac gcagtacact ttggcgatcg accg
//
LOCUS       DAOMUE010000087         6674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3079_length_6674_cov_2.398247, whole genome shotgun sequence.
ACCESSION   DAOMUE010000087 DAOMUE010000000
VERSION     DAOMUE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3079_length_6674_cov_2.398247"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLC71_05245"
     CDS             complement(<1..621)
                     /locus_tag="PLC71_05245"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRM02079.1"
                     /translation="MSKIQMTTPLVEMDGDEMTRILWKMIKDELLLPFIDLKTEYYDL
                     GLEHRNETNDQVTFDSAEATKKYGVAVKCATITPNAARMDEYDLKEMWKSPNGTIRAI
                     LDGTVFRAPITVKGIEPTVSKWKKPITIARHAYGDVYKGVEIKVPGAGKAELVFTGED
                     GTEIRETIHDFDGPGVIQGMHNIDASIASFARSCFTYALDTKQDLWF"
     gene            complement(720..1442)
                     /locus_tag="PLC71_05250"
     CDS             complement(720..1442)
                     /locus_tag="PLC71_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM02080.1"
                     /translation="MGAFYLDIKKDLLDKILSGVYPQGSIIPNEYELAEIYNVSRPTI
                     RKAVQILVDEGYLEKRKKRGTIVCDPTIRQEFAQVIQTFDKEVKEKGLTPSTKVLTFK
                     QEKAEEEVRQKLNLKETEKVYKLVRLRYVDDQPNVLVTTYVPCRLFEHLSEVDFEKES
                     LYQTFHKEGRDITSIQRKLDVILADETCADLLDVKNGDPLFYFHSTGYDESHTAIEYS
                     IAKYRGDRNSFVFQLSNNVYYS"
     gene            1602..2312
                     /gene="deoC"
                     /locus_tag="PLC71_05255"
     CDS             1602..2312
                     /gene="deoC"
                     /locus_tag="PLC71_05255"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRM02081.1"
                     /translation="MKSYTLDDLARMIDHTNLHTDASPKDMEVLCNEAKEHHFAMVAI
                     NQVQSGLCAKLLEGTDIHTGAAISFPLGQTTIASKVFDTKDAIANGANEIDYVVNQTE
                     VKASNWDYIKDEMSQIVAACHEKDVTCKVIFENCYLNDEEKIKLCEIAKAVKPDFIKT
                     STGFGTGGATFDDVKLMKEHVGDAVKVKAAGGIRDAKTFMKMIECGAQRIGTSAGISI
                     IKEFEAMMKEQGVSTLEI"
     gene            2336..2911
                     /locus_tag="PLC71_05260"
     CDS             2336..2911
                     /locus_tag="PLC71_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HRM02082.1"
                     /translation="MNQFNWDQAFTLDFDLNNGLSKHADTTKRYLSQMKNMYQDQEAV
                     EEILKHEDPLIYEFYELGCPERAGDLAFGTTMIHPGKIGNEYYMTKGHFHQIVDTAEV
                     YYTLKGEGYMMLESLDGDWRVERMEKGKALYVPRGYAHRTINTGDEVLTAFYVFDADA
                     GHDYGTIETKGYRHIVCEINGEVTVLDNPRW"
     gene            complement(2997..4709)
                     /locus_tag="PLC71_05265"
     CDS             complement(2997..4709)
                     /locus_tag="PLC71_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HRM02083.1"
                     /translation="MVNITIDNHTIAVPSGTTIMEAAASIHIPIPKLCYLKDINEIGA
                     CRVCVVEIEGQDHLVTSCNNVVEEGMTIYTNSPKVRRNRKHTVEMILSQHDAQCATCV
                     RSGNCTLQTVANDLNIVDSPYKKEICIEEWDTRYPLVRDASKCVKCMRCIQVCDKIQG
                     MHIWDVSGTGARTTVGVSENRDIKTADCALCGQCITHCPTGALRERDDTDKLYRALED
                     KDTIVVAQIAPAVRAAWGESLGLSREEATVEKIVDALRRIGVDYVFDTTFSADLTIME
                     EGTEFVERFTNGDLDMYPMFTSCCPGWVRFIKSQYPQMVNRLSSAKSPQEMFGAVMKT
                     AFAKKMNIDPDRIFALSIMPCVAKKDEREKPLFHGEFAGHGVDCVLTTRELDRLIRAD
                     HIDPKTLKDAAFDTPFTEGTGAGVIFGATGGVMEAALRSAYYLITGKNPEVDAFKQIR
                     GVNKNGWTEAQFEIAGNTIDIAVVSGLQNTRNLMEAIQKREVHYHFVEVMACPGGCVG
                     GGGQPIYEGKELASDRGSNLYFLDKTAHLRFSHENPDIKHLYDEYFGSPGSHKAHQLL
                     HVQK"
     gene            complement(4703..6520)
                     /locus_tag="PLC71_05270"
     CDS             complement(4703..6520)
                     /locus_tag="PLC71_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRM02084.1"
                     /translation="MSRLELRSPNQAQLVVEGLYKDLERRIESSPPGLCPVDMSRAFL
                     EICHAQSCGKCVPCRVGLGQLKNLITDVLDGKATIRTLTEIHETAKSIMESADCAIGY
                     EAANMVYKGAIGFKDDYVEHIMYHRCLSTLKQPVPCVSRCPARVDIPGYIALVREGRY
                     EDAIRLIRKDNPFPTTCGFICEHPCEALCRRNMIDDAVNIRGLKRMAADAAGYVAPPE
                     CAESTGKNIAVVGGGPGGLSAAYFLQLMGHQVTVYEMLPKLGGMLRYGIPKERLDDDI
                     QAILDTGVKVDCGKAIGKDYSIVDLKEKYDAVLITIGASTDKKLGLEGEDAKGVISAV
                     QFLRDVGYGTQESLEGKEVAVIGGGNVSMDAVRTSVRFGAKKVSIVYRRRVADMTALP
                     DEISGAEAEGVEIKTLMAPSRIEKDEEGNVKGIWVTPQMISNIKGGRASVKPTGEDDV
                     FIPCQTLIVAIGQDIEYQHFEEAGVPVQRGKINVEKFGGFEEMPGVFAGGDCATGPST
                     VISAIAAGKVIAANIDEYLGYHHEITADVEIPEPKLEDKPACGRVNLTEREAGERVKD
                     FEGVENCMTEKEACQEAGRCLRCDHFGYGIFKGGRDTKW"
BASE COUNT         1766 a         1504 c         1167 g         2237 t
ORIGIN      
        1 aaaccacaga tcctgttttg tgtctaatgc atatgtaaag cagcttcttg caaaacttgc
       61 aatagatgca tcaatgttat gcattccctg gatcactcct ggtccgtcaa aatcatgaat
      121 tgtttctctg atctctgttc cgtcttctcc tgtaaatact aattctgctt ttcctgctcc
      181 tggaacttta atctccacac ctttgtatac atcaccatag gcatgtcttg cgatcgtgat
      241 cggtttcttc catttgctga cggttggttc gattcctttg actgtgatcg gtgcacggaa
      301 tactgttccg tctaagatcg cacggattgt tccatttgga ctcttccaca tctcttttaa
      361 atcatattca tccatacgcg ctgcatttgg agtgatcgtt gcacatttga ctgcaactcc
      421 atatttcttt gttgcttctg cggaatcaaa tgtaacctga tcgtttgtct cgtttctgtg
      481 ttctaaccca aggtcataat attctgtttt taaatcaata aatggtaata acaattcatc
      541 tttgatcatc ttccaaagaa ttcttgtcat ttcatctccg tccatctcta ctaatggagt
      601 ggtcatctga attttgctca tggtcattcc tcctgcgttg ctgtttattg ttttattcac
      661 tgttttcagc ttattgtgct ttattataca gaaaaaactg atatttgtaa ataaatgtat
      721 taagaataat acacattatt tgaaagttga aaaacaaagc tattccgatc tccccgatat
      781 ttcgcaatcg aatattcaat cgctgtatga ctttcatcat atcctgtgga atgaaaataa
      841 aataaaggat ctccattttt gacatctaac agatccgcac atgtttcatc tgcaaggatc
      901 acatccagct ttctctgtat ggatgtgata tctcttcctt ccttatgaaa tgtctgatat
      961 aaactctcct tttcaaaatc aacttctgac aaatgttcaa acagtctgca tggcacatac
     1021 gttgtaacca gaacattggg ctgatcatca acgtatctca atctgaccag tttataaact
     1081 ttttctgttt ctttgagatt caatttctgg cggacttctt cttctgcctt ttcttgtttg
     1141 aatgtcagaa cttttgtcga tggagtcagt cctttttctt ttacttcttt atcaaatgtc
     1201 tggatcacct gtgcaaattc ctgccggatc gttggatcac atacgatcgt ccctcgtttt
     1261 ttacgttttt ctaaatatcc ctcatccact aaaatctgta ccgccttacg gatcgtcggc
     1321 cggctgacgt tgtagatctc agctaattcg tattcatttg gtataatgct tccttgtgga
     1381 tagactccgc ttaaaatctt atctaataaa tcttttttta tatctaaata aaatgctccc
     1441 attttatact ctctttcttt tttctttcac tttatcggat tttttatttt atgtcaattt
     1501 attttctctt atttcattta cataatattg acataacaat tacataatgt tatattttaa
     1561 ttaacatgaa acaaacatat cagcaaaaat aggaggtttt tatgaaatca tatacattag
     1621 atgatcttgc aagaatgatc gatcacacaa atttacatac ggatgcttct cctaaagaca
     1681 tggaggtctt atgtaatgag gcgaaagaac atcattttgc catggtagcg atcaatcagg
     1741 tgcagtctgg attatgtgca aagttactgg aaggaacgga tattcataca ggagctgcta
     1801 tctctttccc attgggtcag acaacgatcg cttctaaagt ctttgataca aaagatgcta
     1861 ttgcaaatgg tgcaaatgag attgattatg ttgtaaatca gacagaggtc aaggcttcta
     1921 actgggatta tatcaaggat gagatgtcac agatcgttgc tgcctgccat gaaaaagatg
     1981 ttacttgtaa agtcattttt gaaaactgtt atcttaatga tgaggagaag atcaagcttt
     2041 gtgaaatcgc caaagcggtg aaacctgatt ttatcaaaac atccactggt ttcggtactg
     2101 gcggtgctac ttttgatgat gtgaaattaa tgaaagagca tgttggagat gcggtaaagg
     2161 taaaagctgc tggtggaatc cgtgatgcca agacatttat gaaaatgata gagtgcggtg
     2221 cccaacgtat cggaacaagt gctggtatct cgatcatcaa ggaattcgaa gctatgatga
     2281 aagaacaagg cgtttccaca cttgagatct agggcaattt tttaggaggt attttatgaa
     2341 tcagtttaac tgggatcagg cttttacttt ggattttgat ctaaataacg gactttccaa
     2401 gcacgcagat actacaaagc gttatctttc ccagatgaaa aatatgtatc aggatcaaga
     2461 agctgttgaa gaaattctta aacacgaaga ccctttgatc tatgagtttt atgaacttgg
     2521 atgcccagag cgtgctggtg atcttgcatt cggcacaacg atgatccatc ctgggaagat
     2581 tggaaatgaa tattatatga cgaaaggaca ttttcatcag atcgtagaca ctgccgaagt
     2641 ttattataca ttaaaaggcg aagggtatat gatgcttgaa agccttgacg gtgattggcg
     2701 ggttgaacgg atggagaaag gaaaagcatt gtatgttccc cgcggatatg ctcaccgcac
     2761 cattaatact ggagatgaag tcctgactgc tttttatgta tttgatgcag atgcaggaca
     2821 tgattatgga acgatcgaaa ccaaaggata ccgccatatt gtttgtgaga tcaatggcga
     2881 agtaactgta cttgacaatc caagatggta attatgttaa aaagagagaa aatccagaca
     2941 tttagaattt gtatcttcta aatgtctgga ttttctttat taaaaggtat taacagctat
     3001 ttttgcacat gcagtaactg atgtgcttta tgacttcccg gtgatccaaa gtattcatca
     3061 tacagatgtt tgatatctgg attctcgtgt gaaaatctca aatgggctgt cttatccagg
     3121 aagtacaggt tggatccacg gtcagatgcc agttctttcc cttcatagat tggctgtccg
     3181 cctccaccta cacatccgcc tggacaggcc atgacttcta caaaatgata gtgtacttct
     3241 ctcttctgga tcgcttccat cagattcctt gtattctgaa gtccactcac aactgcaata
     3301 tctatcgtat tgccagcaat ttcaaattgg gcttctgtcc atccattttt gttcactcca
     3361 cggatctgtt taaatgcatc tacttctgga ttctttcctg tgatcaggta atacgcagaa
     3421 cgcagtgccg cttccataac tccgccagtt gctccaaaga tcactcctgc tccggttccc
     3481 tcagtaaacg gagtatcaaa ggctgcatct tttaatgtct ttggatcaat atggtcagca
     3541 cggatcagac ggtccaattc tcttgttgtt aacacacagt caactccatg tcctgcaaac
     3601 tcaccatgga ataatggttt ctctctttca tctttcttcg caacacatgg cataatggat
     3661 aatgcaaaaa tacggtctgg gtcgatgttc atcttctttg caaacgcagt cttcatcact
     3721 gccccaaaca tctcctgtgg agatttggca gaagataatc tgtttaccat ctgtggatac
     3781 tgagatttga tgaaacgtac ccatcctgga caacaggatg taaacattgg atacatatca
     3841 agatctccat ttgtaaatct ctcgacaaat tccgttcctt cttccatgat cgtaagatct
     3901 gctgaaaatg ttgtatcaaa tacataatct acaccgatcc ttcgaagtgc atctacgatc
     3961 ttctctaccg ttgcttcttc tcttgataat ccaagacttt ctccccatgc agcacgaact
     4021 gctggtgcaa tctgtgctac cacgatcgta tctttatctt ctaatgcacg atataatttg
     4081 tctgtatcat ctcgttcacg taatgctcct gttggacaat gtgtgataca ctgtccacat
     4141 aatgcacaat ctgctgtttt gatatctctg ttttctgaaa ctccaactgt tgttcttgct
     4201 ccagttccag atacatccca gatatgcatt ccctgaatct tgtcgcatac ttgtatacaa
     4261 cgcatacatt tgacacattt gcttgcatca cgtaccaatg gatatcttgt atcccattct
     4321 tcgatacaga tttccttctt ataaggacta tctacaatat tcagatcatt agcaaccgtc
     4381 tgaagtgtac aatttccgct tcttacacaa gttgcacatt gtgcatcgtg ctgggaaagg
     4441 atcatctcta ccgtatgttt tcggtttctg cgtactttcg gactgttggt atagatcgtc
     4501 attccttctt ctaccacgtt attacaggat gttaccagat gatcctgtcc ttcaatttct
     4561 acaacgcaga ctctgcacgc tccaatttca ttgatatctt ttaaataaca aagctttgga
     4621 atcggtatat gaatggaggc tgctgcttcc atgatcgttg ttccagaagg tacagcgatc
     4681 gtgtggttat ctattgtaat atttaccatt ttgtatccct acctcctttg aaaataccat
     4741 aaccgaagtg gtcacagcga agacatcttc ctgcctcctg gcatgcttct ttctctgtca
     4801 tacagttttc gactccttca aagtctttaa ctctctctcc agcttctctc tctgtaagat
     4861 taactcttcc acatgctggt ttatcttcta acttaggttc tggaatctca acatctgctg
     4921 tgatttcatg atgatatccc agatattcat cgatattggc tgcgataact ttacctgctg
     4981 cgatcgctga aataactgtg gatggacctg tcgcacagtc tccacctgca aatactcctg
     5041 gcatctcttc gaatccacca aatttctcta cgttgatctt tcctctctgt acgggaactc
     5101 ctgcttcttc gaagtgctga tattcgatat cctgaccgat cgctacgatc agtgtctgac
     5161 atgggataaa tacgtcatct tcccctgttg gctttacaga tgctcttcca cctttgatat
     5221 tactgatcat ctgtggtgtt acccatatac ctttgacatt gccttcttcg tctttctcga
     5281 ttcttgaagg tgccatcaat gtcttgatct caacaccttc tgcttctgct cctgaaatct
     5341 catctggaag tgctgtcata tcggcaactc ttcttcgata tacgatgctg actttcttcg
     5401 caccaaatct tacagatgta cgaactgcat ccatggaaac atttccacca ccgatcacgg
     5461 caacttcttt tccttccagg ctttcctgtg ttccgtatcc gacatctctt aagaactgta
     5521 ccgctgaaat aacgcctttg gcatcttcgc cttctaatcc taacttctta tctgtgcttg
     5581 caccgatcgt gatcagtaca gcatcgtatt tttcttttaa atccacaatg gaataatctt
     5641 tgccgatagc ttttccacaa tctactttga ctcctgtgtc taagattgcc tgaatatcat
     5701 catctaagcg ttcttttgga attccataac gtaacattcc tccaagtttt ggaagcatct
     5761 catatacggt tacctgatgc cccatcaact gtaagaaata tgctgcactt aatccacctg
     5821 gtccaccacc tacaacggca atattcttcc ctgtagattc tgcacattct ggcggtgcaa
     5881 cgtatcctgc tgcatctgct gccatacgtt ttaatccacg gatattcacc gcatcatcga
     5941 tcatatttct acggcataat gcttcacatg gatgctcgca gataaatcca catgttgttg
     6001 gaaatggatt atcttttctg atcagacgga tcgcatcctc atatcttcct tcacgtacca
     6061 acgcaatata tcccggaata tccactctcg caggacatct ggatacacat ggcactggct
     6121 gctttaacgt acttaagcat cgatgataca tgatatgttc tacataatca tctttaaatc
     6181 caatggctcc tttgtatacc atattggctg cctcatagcc aatcgcacaa tctgcagatt
     6241 ccataatgga ttttgctgtt tcatgtatct ctgttaaagt tcggatcgtt gctttgccat
     6301 ctaaaacatc agtgatcaga ttctttaact gtccaagtcc tacacgacat ggaacacact
     6361 ttccacagct ctgtgcatga cagatctcca aaaatgctct ggacatatca accggacata
     6421 accctggcgg gcttgactcg attcttcgtt ccaaatcctt ataaagacct tccacaacca
     6481 gctgtgcctg gttcggagac cttaattcca aacgactcat gactcatcct ccttaattcc
     6541 tctatatcag tatattgact cccattatac gcacattctt cgacaaattc aagtgttttt
     6601 tgtgcaattt gtacgttaat ttcgtaacaa tccggttcat ttttgtgtat aatcacgtta
     6661 attttgtaac aatt
//
LOCUS       DAOMUE010000088         6623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3111_length_6623_cov_2.258983, whole genome shotgun sequence.
ACCESSION   DAOMUE010000088 DAOMUE010000000
VERSION     DAOMUE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3111_length_6623_cov_2.258983"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PLC71_05275"
     CDS             complement(<1..560)
                     /locus_tag="PLC71_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3877 family protein"
                     /protein_id="HRM02085.1"
                     /translation="MGYKRLYKNLIDIIKEEQAKLGFRRESIRLYYPLSSLNHFFETQ
                     DTEEQMKNRLHGIPDFIKETLGDIKVTSKKERFCFYIPEEGSNYVHENTNPNEFIKEL
                     VEIVGKHGCKMEDIIALFKQHDKNVFVEEIHNGEFDYLIRFSEENEDPYYYCFHDEGC
                     HIIYHRFLPADYEDFDIPYGMVSKSY"
     gene            complement(582..1040)
                     /locus_tag="PLC71_05280"
     CDS             complement(582..1040)
                     /locus_tag="PLC71_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02086.1"
                     /translation="MKFTMGRTMKILVAILIVAIIAIVMLSPYSFEKYTASSKMIQVT
                     STDTVTKDANGKKKQQVYSYKKDDKKYTEIVNVLDQYSYHFTTKTLTNSSQMSDSSKP
                     QMIMLFGNKMLVISDSGEILLDNHVYRMGYSGGKDAKKMMKSINKILKSE"
     gene            complement(1143..1568)
                     /locus_tag="PLC71_05285"
     CDS             complement(1143..1568)
                     /locus_tag="PLC71_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009289485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02087.1"
                     /translation="MKKRYQIEKYTILFAIGGMAYFLLEVLVRGYSHYSMFLCGGACF
                     LCCGLLNENVKIKISFISQMVLSSVIITVLELITGFIVNIWLKMDIWDYSHLPYNFKG
                     QICLLYSVFWFLISSVAIVLDDFLRYKLFNEEKPRYKIF"
     gene            1797..3179
                     /gene="argH"
                     /locus_tag="PLC71_05290"
     CDS             1797..3179
                     /gene="argH"
                     /locus_tag="PLC71_05290"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRM02088.1"
                     /translation="MAQLWGGRFTKETDQLVYNFNASISFDQKFYKQDIRGSIAHVTM
                     LASVGVLTEEERDQIIDGLKGISKDIQTGAVEITSEYEDIHSFVEANLIDRIGDVGKK
                     LHTGRSRNDQVALDMKLYTRDEIVDIKYLLKELMETLHRIMKEHIDTFMPGFTHLQKA
                     QPVTLAHHVGAYMEMFKRDYSRLTDIYDRMNYCPLGAGALAGTTYPLDREQTASLLNF
                     YGPTLNSMDSVSDRDYVIELLSALSTIMMHLSRFSEEVIIWNSNEYKFVSIDDGFSTG
                     SSIMPQKKNPDIAELVRGKTGRVYGALTSILTTMKGIPLAYNKDMQEDKELTFDAIDT
                     VKGCISLFNGMMNTMTFNKPQMEKSAKHGFTNATDAADYLVNHGVPFRDAHGIVGQLV
                     LLCLDKHISLDELPLDEYQKISPVFEEDIYEAISMKTCVEKRNTIGAPGRDAMKKVIA
                     INDTYLANLK"
     gene            3193..4914
                     /locus_tag="PLC71_05295"
     CDS             3193..4914
                     /locus_tag="PLC71_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02089.1"
                     /translation="MKKKVFLFGLLFSLLLLCGCGVNLTSTVKLNKDFSGTRVMSCTF
                     SSRDFHSYFKGSKEDLNKLIKESCPDALTYTSSSSDGNDTYTFYLRFSSLDDYKKKVR
                     DLLNFSPEITYEYGDSPFVSGLIYKENFTSKDLMTWLYTALYEGKYIDKDSSSDLWDL
                     KSTEISFLGTTYETKDKINIDEMAYVPLSSIHIDTKTKKSGKLARVIEFDLPQQTLDQ
                     NAGKIRSYFAGNDITWENTSDGKTLCISFDANNFSDLAQKTRTVLHSKNSFGTYSSTC
                     SKDNPFTLKINYEESLDLSHFIQKNQKIPVTYTFDEKQMFSDSIKQKEISFTSSVTQP
                     ISTYEIATVWNTSKDIRRKVSFSFEKAVTDHQLSVIKKQFKGQTIDSVNLDGDQNVTL
                     SFVQKGSVSDCNKDFSALFPNSLMKSQAHFSFAGGKKVTFSDKISLPANQNGEKLHGH
                     YVFASVNSKESVTVAVMPEKIVTNTVKQNTSTKAVSALIKSDENIHDLCDFELTGNRL
                     ELSYRGSTAASFWMGALRWMLPVVVLIVIGLFLIRKKVAIREMFGDLKKELAESVNSL
                     IDRINKR"
     gene            complement(5319..5474)
                     /locus_tag="PLC71_05300"
     CDS             complement(5319..5474)
                     /locus_tag="PLC71_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02090.1"
                     /translation="MSTKNNKLRFIDEEKTKELQKDWKGYGKSQVLFGIVVFVIFVLS
                     ALYYYIL"
     gene            complement(5559..5867)
                     /locus_tag="PLC71_05305"
     CDS             complement(5559..5867)
                     /locus_tag="PLC71_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02091.1"
                     /translation="MMSIQTSMATEKDIMSNDVIKQAFDNNVNKISCIPETIETIEVY
                     DSRLDEKEIIKPQSAKASYDYYKTYRTGVSGAYGATGYSKPSVTVSSWSANKMKFKGT
                     "
     gene            6251..6565
                     /locus_tag="PLC71_05310"
     CDS             6251..6565
                     /locus_tag="PLC71_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRM02092.1"
                     /translation="MAKKSFYKIEMLFLKILEKEDCYGYQLTQSIQDITNGQIKIAEG
                     TMYPILYKLQDQGYISDRQVKVGKRQTRVYYHLEPAGKEYLSQLTHDYYQMVNYPSHA
                     VQ"
BASE COUNT         2131 a         1180 c         1034 g         2278 t
ORIGIN      
        1 aaatatgatt tactaaccat tccgtaaggt atgtcaaaat cttcataatc tgctggtaaa
       61 aagcgatgat agatgatatg acatccttca tcgtgaaaac agtaataata tggatcttca
      121 ttttcttctg aaaaacggat cagataatca aattctccat tatgaatttc ttctacaaac
      181 acgtttttat catgttgttt aaataacgca ataatatctt ccattttaca tccgtgtttt
      241 ccaacgattt ctaccagttc tttgataaat tcatttgggt ttgtattttc atggacgtag
      301 ttactgcctt cttctggaat atagaagcaa aatctttctt ttttgctagt aactttgata
      361 tctcctaaag tttctttgat aaaatctggt attccatgaa gtctgttttt catctgttct
      421 tctgtatcct gtgtttcaaa aaaatggttt aacgaactta atggatagta cagacggata
      481 ctttctcttc gaaatcccaa ttttgcctgt tcttccttga taatgtcgat caaattttta
      541 taaagacgtt tatatcccat gatgtcttcc cctattatat tttattcact ctttaaaatc
      601 ttgttgatgc ttttcatcat ttttttcgca tcttttccac cagaatatcc catgcggtat
      661 acatgattat ctagaaggat ttctcctgaa tcactgatca caagcatttt atttccaaat
      721 aacatgatca tctgtggctt actagaatca ctcatctgag atgagttagt taatgtcttt
      781 gttgtaaaat gataagaata ctgatctaaa acattaacga tctctgtgta ttttttatca
      841 tctttcttat atgaatatac ctgctgtttt ttcttaccat ttgcatcttt tgttacagta
      901 tcagtcgaag taacctgaat cattttagat gatgctgtat atttttcaaa agaatatggt
      961 gagagcataa cgatcgcaat gattgctacg atcaaaattg ctacaaggat cttcattgtc
     1021 cttcccattg taaatttcat tttgttgttc ctccaatcta ctatttgtac ctataataga
     1081 ttaacagaat ttccctcaaa tgtcgatagc tttccctgtg aactgctatt tatactacat
     1141 tcttaaaaaa tcttataacg tggcttttct tcattgaata atttgtatct tagaaaatca
     1201 tccagcacga ttgctacact tgaaataaga aaccaaaata ccgagtacaa taaacagatc
     1261 tgccccttaa agttatatgg aagatgcgaa taatcccaaa tatccatctt aagccatata
     1321 tttacaataa aacctgtaat aagttctaat accgtgatga tcacagaaga taataccatc
     1381 tgactaataa aacttatctt gatctttaca ttttcgttta gaagcccaca gcaaaggaaa
     1441 catgccccac cacataaaaa catcgaataa tgtgaatatc cacgtactaa aacttccagc
     1501 aaaaaatatg ccattcctcc aatcgcaaac aggattgtat atttttctat ctgatatctc
     1561 tttttcatag tcaccgccca ttttcataac tttcttttta ttgtgcgtta atttttctct
     1621 gtctattcta tcttaaattc aaaaaatcta tatgatgaga gtatcaaaaa taaatttttc
     1681 ccctttgatg caaaaaattt gtggcaaaat cgagttattt catgtagaat agaaaagtaa
     1741 attcatttga ttttatgata aagaataaaa atatatacag aaaagaggaa acacttatgg
     1801 cacaattatg gggcggtcgc ttcacaaaag aaacagatca gttagtttac aactttaatg
     1861 catctatttc ctttgaccag aaattttata aacaagacat ccgcggaagt attgcacatg
     1921 ttacgatgct tgcaagtgtt ggtgtattaa cagaagaaga acgtgatcag atcatcgatg
     1981 gtttaaaagg aatttcaaag gatatccaaa ctggagcagt tgagatcaca agtgaatatg
     2041 aagatatcca tagctttgta gaggcaaacc ttattgatcg catcggtgat gttggaaaga
     2101 aacttcacac tggaagaagc cgtaatgatc aggtcgcttt agatatgaaa ctttatacaa
     2161 gagatgaaat tgttgatatc aaatatttat taaaagaatt aatggaaaca cttcatcgta
     2221 tcatgaaaga acacattgat acatttatgc ctggatttac acatcttcag aaagctcagc
     2281 ctgtcactct tgctcaccat gtaggtgctt acatggaaat gtttaaaaga gattacagcc
     2341 gtttaactga catttatgac agaatgaact actgtccttt aggagctggt gctcttgcag
     2401 gtactactta tccattagac cgtgaacaaa cagcttcttt gcttaatttt tatggtccta
     2461 cattaaacag tatggattct gtatctgacc gtgattatgt gatcgagctt ttatctgctc
     2521 tatctacgat catgatgcat ttaagccgtt tcagtgaaga agttatcatc tggaactcta
     2581 acgaatacaa atttgtatcc attgatgacg gattcagtac aggaagcagt atcatgcctc
     2641 agaaaaagaa tcctgatatt gctgaattag ttcgtggtaa gacaggacgt gtctatggag
     2701 ctttaacttc tatccttaca acaatgaaag gaatccctct ggcatataac aaagatatgc
     2761 aggaagataa agaacttaca tttgacgcga ttgatactgt caaaggatgt atttctttat
     2821 ttaatggaat gatgaatacc atgacattta acaaaccaca gatggaaaaa agtgcaaaac
     2881 acggatttac aaatgcaact gatgctgcgg attatcttgt aaatcacggt gttcctttcc
     2941 gcgatgctca cggaatcgtt gggcagcttg tacttctttg tcttgataaa catatttctc
     3001 tagatgaact tcctctagat gaataccaga agatcagtcc tgttttcgaa gaagatattt
     3061 atgaagccat cagcatgaaa acttgcgttg aaaaacgtaa tacgatcgga gctccaggac
     3121 gtgatgctat gaaaaaagtt atcgcgatca atgatactta tttagctaat ctcaaataat
     3181 aaggagaccc ttatgaagaa aaaagttttt ctttttggat tactgttctc actgcttctc
     3241 ttatgcgggt gcggtgtgaa tcttaccagt acagtaaaat taaataaaga cttttcaggt
     3301 acaagagtga tgtcttgtac cttttcttcc cgcgattttc atagttattt caagggaagc
     3361 aaagaagatc ttaataaact catcaaagaa tcctgcccgg atgctcttac ttatacttca
     3421 tcttcttctg acggaaacga tacgtatact ttctatcttc gtttttcttc tcttgatgat
     3481 tataagaaga aagttcgtga tcttcttaat ttttccccag agatcactta tgaatatgga
     3541 gattccccat ttgtttccgg tcttatttac aaagaaaatt tcacatctaa agatctgatg
     3601 acatggcttt atacggcttt gtatgaggga aaatatattg acaaagactc ctcttctgac
     3661 ctatgggatt taaaaagtac ggagatttct ttccttggaa cgacttatga aacgaaagat
     3721 aagatcaata ttgatgaaat ggcttatgta ccattatctt ccattcatat tgatacaaag
     3781 acaaagaaat ctggaaaact tgcaagagtg atagaatttg atcttccaca acaaacatta
     3841 gatcaaaatg caggaaagat ccgttcttat tttgctggaa atgatatcac ttgggaaaat
     3901 acatccgatg gaaaaacttt atgtatcagt tttgacgcca acaatttctc tgatcttgca
     3961 cagaaaacaa gaactgtcct tcattcaaag aattcttttg gaacttacag ctccacatgc
     4021 agcaaggaca acccttttac actgaagatc aactatgaag aatctctgga tctctctcat
     4081 tttattcaga aaaaccaaaa gattcctgtt acttatacat ttgatgaaaa acagatgttt
     4141 agtgattcga tcaaacaaaa agaaatcagc tttacttctt ctgttacaca gccaatttca
     4201 acttatgaga ttgctacggt atggaacaca tccaaagata tccgtcgtaa agtgtctttt
     4261 tcttttgaaa aggctgtgac tgaccatcag ttatctgtga tcaaaaagca gttcaaagga
     4321 caaaccattg attctgtaaa tttagatggt gatcagaatg ttacgttatc ttttgtacaa
     4381 aaaggaagtg tttcagactg taataaggat ttctctgctt tgtttccgaa tagtttgatg
     4441 aaatcacaag cgcatttttc atttgctggt ggtaagaagg tcacattttc tgataagatt
     4501 tcattacctg cgaatcaaaa tggtgagaaa cttcatgggc attatgtatt tgcttctgtg
     4561 aattcgaaag aatctgttac tgtagctgtt atgcctgaga agattgtgac aaatacggta
     4621 aaacagaata catctaccaa agcagtctct gccctgatca agtcggatga gaatattcat
     4681 gatctttgtg attttgaatt gactggaaat cgtttggaat taagctatcg tggaagtact
     4741 gctgccagct tttggatggg tgcattaaga tggatgttgc ctgttgttgt tttgatcgtg
     4801 attggtttat ttttgattcg aaagaaagtg gctattagag aaatgtttgg ggatttgaaa
     4861 aaggaattag ctgaaagtgt taattcactt attgatagga ttaataagag gtaagacact
     4921 aatctttcat cccacaacct cttgatttac aactactagt actgacttgt agttcgctac
     4981 acttggcggt aaatacccat gactggactt gcaccaacga gattagtgcc atgctcggca
     5041 cacaaataag taaggactgc cataatgaca gtcctctcca gcattaagtt gctattagat
     5101 taacttaagc tctacagaat ttaacgaact tcaattcctc cccctgtatt gcaataaaat
     5161 tcaaatgaca gcgaaacttg ctttgtaaaa ccagaagaat tttttatgac tcctaaacat
     5221 aaaatttctc ctgttctgcc accatcaatc acacgttttg taattgtagg tgacttcttc
     5281 catgaaaatc cataaccact tacttaaaaa cttaaaatct ataaaatata gtaatataat
     5341 gctgataaca caaaaatcac aaaaaccaca attccaaata atacttgaga ttttccatat
     5401 cctttccaat ctttttgtaa ttcctttgtt ttttcttcat caataaatcg aagcttatta
     5461 ttttttgttg acatatgaac acctcctcat agccatatat catggtaacg atatagtctt
     5521 agttcctgtc atagtatatg ttcccccagc tgtaagtttc atgtaccttt aaatttcatt
     5581 ttatttgctg accatgaaga taccgttaca gatggttttg aatatcctgt cgctccatat
     5641 gcacctgaaa cacctgttcg atatgtttta taataatcat aagaagcctt tgctgactgc
     5701 ggtttaataa tttctttttc atctaatctc gaatcatata cctctatcgt ttctatagtt
     5761 tctggaatac aacttatttt attcacgtta ttatcaaatg cttgttttat cacatcgtta
     5821 ctcataatat ctttttccgt tgccatactt gtttgaatag acatcattaa tattgcactc
     5881 attaataaaa acgctacaat catttttggt ttagatttga tcctatcata ttcctccttt
     5941 atttacttta ataccttcta tgtttatgtt accccttttt cttccataca atataatcca
     6001 ggattttctt cattgtatgg attttttacc gattcttatt tttcgtaccc ccccttttat
     6061 tttctaattt cataaatact atgttaattt acgtttaact atatacttta ttatttaata
     6121 tattatttat tttattatac aattttctat gtattattgt caactattac ttatataatt
     6181 tgtgtattgt tttatcatat atataatatt acaattagtc atagaaaatt tattaagtag
     6241 tggagaaaat atggcaaaga aaagttttta taaaattgaa atgctgtttt taaaaattct
     6301 agaaaaagaa gactgttacg gataccaact aacacaatcc atacaagata tcacaaacgg
     6361 acaaatcaaa attgcagaag gtaccatgta tccaattctt tataaattac aagatcaagg
     6421 atatatctct gatcggcagg ttaaagttgg gaaacgccaa accagagttt actatcattt
     6481 agaacccgct ggaaaagaat atctttctca attgacacat gattattatc aaatggtgaa
     6541 ttacccctcc catgcggtac agtagtttct atggaagtgg cttctaacag tcgtgacagt
     6601 tacccatttc tgaccaaaag att
//
LOCUS       DAOMUE010000089         6582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3139_length_6582_cov_2.324345, whole genome shotgun sequence.
ACCESSION   DAOMUE010000089 DAOMUE010000000
VERSION     DAOMUE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3139_length_6582_cov_2.324345"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PLC71_05315"
     CDS             <1..1057
                     /locus_tag="PLC71_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008751557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRM02093.1"
                     /translation="MKPVWGDDYAICCCVSATKTGKEMQFFGARANLAKCLLYAINGG
                     VDCKSKQQVGPSYKPIMSEYLDYDEVMEKYDVMMDWLVDLYVNTLNLIQYMHDKYYYE
                     AAELALMDTELERTFATGIAGFSHAVDSLSAIKYAKVKAIRDEDGLVVDYEIEGDFPK
                     YGNDDDRADDIAVWLLRTFLAKIKKHHTYRNSEPTTSILTITSNVVYGKATGTMPDGR
                     KAGEPLAPGANPSYGAEQNGLLASLNSVAKLPYEWALDGISNTQTINPDALGHEEGER
                     INNLVNVMDGYFDQGAHHLNVNVFGKEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDL
                     TREQQMDVISRTCHATM"
     gene            1080..1832
                     /gene="pflA"
                     /locus_tag="PLC71_05320"
     CDS             1080..1832
                     /gene="pflA"
                     /locus_tag="PLC71_05320"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HRM02094.1"
                     /translation="MNEKITGKIHSIETFGLVDGPGVRFIAFMQGCRMRCKYCHNPET
                     WQENCGEDWQAEALLKRALRYRMYWGSDGGITVSGGEPLLQIEFLIEFFKLAKQEGIH
                     TALDTSANPYTKEEPFHSKWLELMKYTDLVILDLKEMDEEKHKKLTGCPNSNILAMAQ
                     EISDLGVDLWIRHVLVPDLTDDEKGLADLKHFIDGLKTVKRVEILPYHTLGLFKWEKL
                     GIKYPLEGVNPPSEEAVAKAEKILGIEKDSQK"
     assembly_gap    1997..2006
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2161..2526
                     /locus_tag="PLC71_05325"
     CDS             2161..2526
                     /locus_tag="PLC71_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRM02095.1"
                     /translation="MKLIELSEVEILIMKSIWKLGDGITVYEIIDYLDQVYDRKYTRS
                     TVKTYITKLKKKGFVDTQVKGNYSYITAKITEEMYREEQMELMKDFWHDGSMKELVQT
                     LTHTISKEELKDLINDLDD"
     gene            2510..3112
                     /locus_tag="PLC71_05330"
     CDS             2510..3112
                     /locus_tag="PLC71_05330"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRM02096.1"
                     /translation="MIWMIKKIYIYVISMILLNGLVGSISYLCFRKIRQQLERKGLIS
                     MCITVLRGAVLSFLMPIVIVLIYFIYYVYEEDYTMFALSPLVGWVFFVVGIIWTIGFL
                     KAILKTIRIQAQMNQLRKRACVCSRSILDCKNQWKEEVGVRRNVEIKTVYGLAVPIIC
                     GFLKPMILLPEREYNKEELKIICIHELIHCKQKKIFYNNL"
     gene            3237..3767
                     /locus_tag="PLC71_05335"
     CDS             3237..3767
                     /locus_tag="PLC71_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02097.1"
                     /translation="MAGTFLCIGLSFVVVAVIILSTIGYHKIYVETVWATEIEEDLPE
                     EETEYTMDLKEYTAKRIGTNLAVTKLKQNIKKGEEIDVVQPLNAKTRLETKELRLKKG
                     ERIELTVFSSEEIQRDDKDFVAGIIDETGKERYVMHAVDIIHDFYVKKDGKYKVFVEN
                     RYKKKIKIGIAICLEK"
     gene            3771..4895
                     /locus_tag="PLC71_05340"
     CDS             3771..4895
                     /locus_tag="PLC71_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02098.1"
                     /translation="MRKYVVRFMVFYILSIGCLLGIIIYSYQNKIDRHNEQVEIEYPG
                     VWKLSDEYDNRNSSYMEFLHQKNSLSILKKVYKELEKAELYREYSRQILSYHEYFRGN
                     EKLASGSYNDKTEDGYLTNLTVLQMPEKMISEKKIEQHIEKGRCFTLKEYQRPLNQEI
                     PLIAGYDYQKVFHLNQKIKAGYLGTENCTFKIVGFLHKNTRLSSELNLDETLIMPTVS
                     KMKTMKQENEKILMSVKCSGYFCYKNEKEYRQISNLLKRIRRETGYRYVIPQMKWKSN
                     NLLPMTTEKALIFMSLNLLLEFIVVYKVLAVNTRKVKTLVYKTVALCTSGSIFANVFL
                     RFLFPKHIKIIQIYSSDFVAYMIIVFLLCAGMIGLKEKRT"
     gene            4892..5791
                     /locus_tag="PLC71_05345"
     CDS             4892..5791
                     /locus_tag="PLC71_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM02099.1"
                     /translation="MMEKILLGSSYLAQAIVMATIAFAVISTIYYLRYNKKIICQPGW
                     IIKYIFLVYIATVGMVTRVLDITSWSYGGFTAWNIVPFVNESWKLMLLNTLMFLPMGL
                     FVPIFIKKIKWNYGKAIIAGALISLMIEFVQVVFAGRIGDIDDIIFNTLGFLIGYILE
                     NLLQKILLKKQLGFGTEALILVMINGFLSIPFYEKTVSIGDMILDSLNLNSWSGNSGY
                     ILSYNGIHYTMIPCIILAVISWAIAHRHPKDYGAKTAKIISIFMIVCFIGNFIQILSQ
                     DSIIPMLAASIVCAVISRKTIRY"
     gene            5854..6114
                     /locus_tag="PLC71_05350"
     CDS             5854..6114
                     /locus_tag="PLC71_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02100.1"
                     /translation="MKKLSILLAGVCLTAALYGAGTIYGQNKTNTAKQSEDKVTTNKY
                     QGVTIKDKAKGKVTKQQVKQLKKEHSGKDEGNIIIWEAEEAE"
BASE COUNT         2487 a          910 c         1333 g         1842 t
ORIGIN      
        1 tatgaaacca gtatggggag atgattatgc gatctgctgc tgtgtatctg caactaagac
       61 tggaaaagaa atgcagttct tcggagcaag agcaaacctt gcaaaatgct tattatatgc
      121 gatcaacggt ggtgtggact gcaaatcaaa acagcaggta ggaccttctt acaaaccaat
      181 catgtctgaa tatttagact atgacgaagt tatggagaaa tatgatgtta tgatggactg
      241 gttagtagat ctttatgtga acacattaaa cttgatccag tatatgcatg acaaatatta
      301 ctatgaagca gcggaattag cattaatgga tacagaactt gaaagaacat ttgcaacagg
      361 aatcgcagga ttctctcatg cagttgactc tttaagtgcg atcaaatatg caaaagtaaa
      421 agcgatccgt gatgaagatg gattagttgt agactatgaa atcgaaggag acttccctaa
      481 atacggaaac gatgatgaca gagctgatga tatcgcagta tggctgttaa gaacattctt
      541 agctaagatc aaaaaacatc acacatatcg aaactctgag cctacaacat ctatccttac
      601 gatcacatct aacgttgtat acggaaaagc aacaggaaca atgccagatg gtagaaaagc
      661 aggagaacct ttagcaccag gagcaaaccc aagttatgga gcagaacaga atggattatt
      721 agcatcctta aactctgtag ctaaacttcc atatgaatgg gcattagatg gtatttctaa
      781 tacacagacg atcaacccag atgcattagg acatgaagaa ggcgaaagga tcaacaatct
      841 tgtaaatgtt atggacggat actttgatca gggagctcac cacttaaatg taaacgtatt
      901 tggtaaagaa aaactgatcg atgcgatgga acatcctgaa aaagaagaat atgcaaactt
      961 tacgatccgt gtatctggat atgcagtaaa attcatcgac ttaacaagag aacagcagat
     1021 ggacgttatt tccagaacat gccatgcaac aatgtaattt aaattagtgg aatagaacta
     1081 tgaatgaaaa aataaccgga aaaatccatt ccatcgagac ttttggtctc gtggatggtc
     1141 ccggtgtgcg gtttatcgct ttcatgcaag gctgccggat gagatgcaaa tattgtcaca
     1201 atccggagac ttggcaggaa aactgcggtg aagactggca ggcagaagcc ctcttaaaga
     1261 gggctttgcg ttaccgcatg tactggggaa gtgatggagg aattacagtc agcggtggag
     1321 aaccccttct acagatcgag ttcttgatcg aattttttaa attggcaaaa caagaaggaa
     1381 tccatactgc acttgatacc tctgctaatc catatacaaa agaagaacca tttcacagca
     1441 aatggttaga attaatgaaa tacactgacc ttgtcattct agacttaaaa gaaatggacg
     1501 aggaaaaaca taagaaattg acaggctgtc caaacagtaa tattttagca atggcacagg
     1561 aaatctcaga cctcggagta gatctgtgga tccgtcacgt gcttgtccca gatttaacag
     1621 acgatgaaaa aggactagca gacttaaaac attttatcga tggactaaaa acagttaaga
     1681 gagtagaaat cttgccatac catacacttg gactctttaa atgggaaaaa ttagggataa
     1741 aatacccatt agaaggcgta aatccaccaa gcgaagaagc tgtagcaaag gcagaaaaga
     1801 ttcttggtat tgagaaagat tctcaaaaat aaaggtagac aaaacatcca ggataggata
     1861 ttataataat agttagttaa gactaactaa cataaaacaa aaacaaaaca taacaaagct
     1921 agaaaatctg accagacttc cttgggtcgg attttttatt tctttatagg aaagttcgcc
     1981 tttggctcct ttccaannnn nnnnnncata catatgcgaa ggctgagaaa aagacaaaag
     2041 tgaaaaatca aagatagttt ttgtatacaa attcgttata attatcacta atcttgacaa
     2101 agacaagctt gatttatata atggttataa taataaccag tttagatgga aggaagaaca
     2161 atgaaattaa tagaattatc agaagtagaa atacttatca tgaaaagtat ttggaaactt
     2221 ggggatggga ttacggtata tgaaattatt gattatctag atcaagtata tgataggaaa
     2281 tatacaagaa gcacagtaaa gacatatatc acaaaattaa agaaaaaagg atttgtagat
     2341 acacaagtga aaggcaatta cagctatatc acagcaaaga tcacagaaga aatgtatcgt
     2401 gaagaacaga tggaattgat gaaggatttc tggcatgatg ggtcgatgaa agaattggtt
     2461 cagacgttaa cacacacaat atcaaaagaa gaattaaagg atttgatcaa tgatttggat
     2521 gattaaaaaa atatatattt atgtcatcag tatgatactg ctgaatggtt tggtgggaag
     2581 tatttcgtat ctttgctttc gcaagataag gcaacaacta gagcgaaaag gactgatcag
     2641 tatgtgcatt actgttttaa gaggagcagt tttatcattt ctgatgccaa tcgtaattgt
     2701 attgatctat ttcatttatt atgtatatga agaagattat acaatgtttg cactgtctcc
     2761 attggtagga tgggtatttt ttgttgtagg aatcatttgg acgattggat ttttaaaagc
     2821 tatactgaaa acaatacgta ttcaggcaca aatgaatcaa ttacgaaaga gggcatgtgt
     2881 atgcagcaga tctattttag attgcaagaa tcaatggaaa gaagaagtag gtgttcgtag
     2941 aaatgtcgaa attaaaaccg tatatgggtt ggcagtaccg attatttgcg gatttctaaa
     3001 accgatgatt ttgcttccag aaagagaata taacaaagaa gaattaaaga ttatatgcat
     3061 ccatgagttg attcactgta agcaaaaaaa gatattttac aacaatctgt gaaatcggaa
     3121 tatcaccatt tgcaaaagga gcatatctgt gcgcagcatt aggagaagac aaaagtcaat
     3181 taaagacacg aattttaaga attaaatcaa ttgaaaacaa aaatacaagg aatgtaatgg
     3241 caggaacatt cctttgtata ggattatctt tcgtggtcgt tgcggtaatt attctaagca
     3301 cgatcggcta tcataaaatt tatgtagaaa cagtctgggc aacggaaata gaagaggatc
     3361 ttcctgaaga agagacagaa tatacaatgg atttgaaaga atatactgca aaaaggatcg
     3421 ggacaaactt agcagttaca aagctaaaac aaaatatcaa aaaaggagaa gaaatagatg
     3481 tagttcagcc attaaacgcc aaaacaaggt tagaaacaaa agagctgaga ttaaaaaaag
     3541 gtgagagaat tgagctgacc gttttttcat ctgaagaaat acaaagagac gataaggatt
     3601 ttgtggcagg aattatagat gagacaggaa aagaacgata tgtgatgcat gcggtcgata
     3661 ttatacatga cttttatgtg aaaaaagatg gaaaatataa agtattcgtt gaaaatcgtt
     3721 ataaaaagaa aataaaaata ggaatagcga tttgtttgga gaaatgataa atgaggaaat
     3781 atgtagttcg ttttatggta ttttatatat taagcattgg gtgtttactg ggaattatta
     3841 tatattcgta tcaaaataag attgatcgtc ataatgaaca agtggagata gaataccctg
     3901 gagtatggaa gttatcagat gaatatgata atagaaattc tagctatatg gaattccttc
     3961 atcagaaaaa ctctctcagt attttaaaaa aagtatataa agaattagaa aaagcagagt
     4021 tatacagaga atattccaga cagattctat cttatcatga atattttcgt gggaatgaaa
     4081 aacttgcatc tggatcatat aatgacaaaa cggaagatgg atatcttaca aatttaactg
     4141 tacttcagat gccagagaaa atgataagtg aaaagaagat agaacaacat attgaaaaag
     4201 gtcgttgctt tactttaaaa gagtatcaaa gacctttgaa tcaagagatt ccattaattg
     4261 caggatatga ttatcaaaaa gtttttcatt tgaatcagaa aattaaggca ggatatttgg
     4321 gaactgaaaa ttgtacgttt aaaattgttg ggtttttaca taagaatact aggttgtcat
     4381 cggaattaaa tcttgatgaa acattgatta tgccaacagt aagtaaaatg aaaacaatga
     4441 aacaagagaa tgaaaaaata ttaatgtcag taaaatgttc aggttatttt tgttataaga
     4501 atgagaaaga gtatcgtcaa ataagtaatt tgctaaaaag aatacgcagg gagacaggtt
     4561 atcgttatgt gataccgcag atgaaatgga aatcaaataa tttattacca atgacaactg
     4621 aaaaagcatt gatattcatg agtttaaatt tgctactaga attcatcgtt gtatataaag
     4681 tactggctgt gaatacaaga aaagtgaaaa cattggtata taaaacagta gccctatgta
     4741 caagtgggag tatatttgca aatgtatttt tgcggttctt gtttcctaaa cacataaaaa
     4801 taattcaaat atattcttct gattttgtgg cttatatgat aatagtattt ttgttatgtg
     4861 caggaatgat tggattaaag gagaaaagaa catgatggag aagattctgc ttgggagtag
     4921 ttatctagct caagctattg ttatggcaac gattgcattt gcagtcataa gcacaattta
     4981 ctatttacga tataataaaa agattatatg tcagccagga tggatcataa aatacatatt
     5041 tttggtttac atagcaaccg ttggcatggt cacgagagta ttagacatca catcatggtc
     5101 atatggagga tttacagcct ggaatattgt accatttgtg aatgaaagct ggaaattgat
     5161 gctgttaaat acattaatgt ttctgccaat gggacttttt gttcccatat ttataaagaa
     5221 aataaaatgg aactatggaa aagcaataat tgcaggggca ttgatatctt tgatgattga
     5281 gtttgtacaa gttgtatttg caggaagaat tggtgatatt gatgatatca tttttaatac
     5341 attaggattt ctgatcgggt atatcctaga gaacctgttg caaaaaattc ttttgaaaaa
     5401 gcaattagga tttggaacag aggcattgat actggttatg atcaatggat ttttaagtat
     5461 cccattttat gagaagacgg tgtcaattgg agatatgatt ttggatagtt tgaatctcaa
     5521 cagttggtca ggaaatagtg gatatatatt gtcatataac ggcatccatt acacaatgat
     5581 cccatgtata atactagcag tcatcagctg ggctatagca catagacatc caaaagacta
     5641 tggtgctaaa acagcgaaaa taatcagtat atttatgatt gtttgcttca ttggaaattt
     5701 tattcaaatt ttaagccaag atagtattat accaatgctt gctgcatcca ttgtgtgtgc
     5761 ggtaataagt cgaaaaacta ttagatatta gggatttgat attccatgaa aaatatgaga
     5821 tactatagta taaaacgtta aggagaataa gatatgaaaa aattatcaat actacttgca
     5881 ggggtatgct taacagctgc gttatacggc gcaggtacaa tctatggaca aaacaaaacc
     5941 aacaccgcaa aacaaagcga agacaaagtc acaacaaaca aataccaagg cgtaacgatc
     6001 aaagacaaag ccaaaggaaa agtgacaaag caacaagtta agcagttaaa aaaagaacat
     6061 tccggcaaag atgaaggaaa catcatcatc tgggaagcag aagaagccga atagaggggg
     6121 cgaagaaatg ttaataccag atttaatcat acagctgatt acagtagtta ttggagtggt
     6181 actatataca gctatattta aaatagtaaa aaatgtgatt cataaaataa tacattaaag
     6241 aggacagtat tagttttagg gaaacagttg tgatataacc aacacatttt attattcaat
     6301 agttctttcc aataaacttc aagtatgtaa gatatcataa caatcaaata gttcccgtcc
     6361 acaacgatta gcgttttatc attatttttg accgattcgc agccgacatg gtgtattgtc
     6421 tgagcgatat ggaagtcatt ttggacttcc ataagcgagt tatacaagga atgtcggcga
     6481 atcagcgatc ggtcaaaaat gatgaataaa acgcgtgttg tggacgggaa ctatttgatt
     6541 gttatgatat cttatatact caaagtctat ggtaaagaaa ct
//
LOCUS       DAOMUE010000090         6581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3141_length_6581_cov_2.256359, whole genome shotgun sequence.
ACCESSION   DAOMUE010000090 DAOMUE010000000
VERSION     DAOMUE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6581
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3141_length_6581_cov_2.256359"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLC71_05355"
     CDS             <1..672
                     /locus_tag="PLC71_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRM02101.1"
                     /translation="KTWIFALGQAFFSLSVAGNGTLIYGSYLSDEEDIPSSAARVAFF
                     DTVAAMLAALVIIPAMATTGATLDQGGPGLLFIYLPNLISSMPGSTIIAIIFFVAVLF
                     AGMTSLINLYEAPIATVQEKLHVGRKTACVIIAVIGVIVSLLIQGIVSDWMDILSIYI
                     CPLGAGLAGIMFFWIAGKKYVETQVNKGRETKFTKMYYPICKYIYVPICILVLVLGIA
                     LGGIG"
     gene            890..1267
                     /locus_tag="PLC71_05360"
     CDS             890..1267
                     /locus_tag="PLC71_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRM02102.1"
                     /translation="MELKELTSAEQVVMKCIWDYGKDMPLQQLIENLKQDYGKDYKRT
                     TIRTFVLHLEEKGYVTTYQIGKYSYINYLIEEQDYKEQELEKTKNFWYKESGFDLVKT
                     LYKGKLNEGKLKKLKKLLEEIDD"
     gene            1279..2658
                     /locus_tag="PLC71_05365"
     CDS             1279..2658
                     /locus_tag="PLC71_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRM02103.1"
                     /translation="MIDIREILVRIFSAIFLSMCTGSCMFVFWMVLRKFFADKIRPKV
                     YDLILKIILIAYYVPAGYLLVNIFFDNGYVFDFTGTIINAFYAISLFWLAGAIATVLK
                     FGERTFRIRREKERCFPCKMYVQKIFEDCKRELGIRRSIEVLQGYRIQIPMTAGILKP
                     CVFLPVEDMEEEQLKTCIYHELTHYKKHDIFWNYIACLMVCIHWYCPWIRTVFRKNDE
                     WSEVICDLSAIGYVGSAKRYFTTIFEMSQKSQGIKAYRAACLFESRDSLEQRIYYAMM
                     YRKQKKIKYAAVIAMTVIFCGMSVTSILAASKGYQKAYTKWVQETEIEIEEPDDEEEE
                     RISYEEKTGVLGNDKSETIKKINFKKMDAMTLETYVKAGKRLRYKGLTPKRNENIEIF
                     ITSQKNFKGIKVGIIDSQNRIRYIQDRGRDLVEHRFKIEKEGKYDVFIEMEDKKDVKI
                     VGMINIDDD"
     gene            2687..3133
                     /locus_tag="PLC71_05370"
     CDS             2687..3133
                     /locus_tag="PLC71_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02104.1"
                     /translation="MIKYIKIMVTVFTLLISCSVTNIFASDTNDVIEEKIDISENIID
                     EVGEEDFELLSNSITVNWTVKNGVRKKTKEFYKKKGSSINIRLQIAPKTKSVKVGYLD
                     ADKVKHYKTVKAEINHNFIIQKTGYIEVFVENSSGKTVTASGTYTK"
     gene            3311..>3658
                     /locus_tag="PLC71_05375"
     CDS             3311..>3658
                     /locus_tag="PLC71_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02105.1"
                     /translation="MKLKQFVCKAASIAMCAMIIGTTVVSVNVKADTKATESTVALDT
                     HDDDGVAGILEQDDFDTLEEYQKYLETHPKVQTRQSRVSANKNVKAAATLRYKIKGLT
                     NTAAIQKTYIGSTY"
     assembly_gap    3659..3692
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    3907..3958
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3959..4529
                     /locus_tag="PLC71_05380"
     CDS             <3959..4529
                     /locus_tag="PLC71_05380"
                     /inference="COORDINATES: protein motif:HMM:NF036612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helveticin J family class III bacteriocin"
                     /protein_id="HRM02106.1"
                     /translation="DYTEIPRFSHMSYANKSGTSIGEVKRVDAALSSDRKKVFFWVMD
                     NTGEIQYSFYNAEKLNAELDKKESEESKFVPCTSSAVKSACYGSFRQSGSNRVLPNDS
                     CQGLEFSDGDSIYIIGGAAGQKPGIAKLTGSGSSYKYSCLVTATHNNFGGNAESEGIQ
                     LKGDYVYFGISDKQSSDKACIYSIPKSAF"
     gene            4599..5837
                     /locus_tag="PLC71_05385"
     CDS             4599..5837
                     /locus_tag="PLC71_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HRM02107.1"
                     /translation="MVRKRMVSTVMSLMMAAAVLTTVPVTNNVKAADKEITSGDYTYV
                     KESNGKTSYAVLTSYKGSETNLVIPEELDGLQVKAISQGFEKNLKIKSIILSKNIALA
                     KETHRDLEVLNEIETLEEIRVAKDNLSYQAQDGVLYSKDKKQLFSYPKSKKSETYNMP
                     ASVKKVEESNALTNLKYLKNLTLSKNLSVTPSCNDSSIESVTIPGQIGGIDESSFENC
                     NKLNKVTITKGLRFIDDYAFFECKALKEIKLPEGLQSIGVGVFYRTGIKQLTIPGSVV
                     KIDVIDKSIKLSKPSYLKKFKRDSGAIYYEARATIKASGKKAVTYKASRITKIKAKTS
                     KVTIQKGKTTKLQTRVYISKKLKKGYLDPEILKFTTSNKKVVKVSSKGTIKGLKKGKA
                     TVTVKLRTTGKTYKVNVKVK"
     gene            6029..>6581
                     /locus_tag="PLC71_05390"
     CDS             6029..>6581
                     /locus_tag="PLC71_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02108.1"
                     /translation="MPGYILHLTAAQMFLKTQKGQEFLKTKQDKNDFLIGNLLPDTTK
                     IKARSHFRDPKYHDRMIEYPETSWFIKKYKHLLSNSSVVGYLFHLYIDRRFFKDYMPR
                     IVEFRNVQDEREERRDMVKDVLLKRTGQRLSKWDFFSEKYYYGDYTKMNMYLVNRYQI
                     PTTLDPNISNPGIKEVDYEDVKQV"
BASE COUNT         2526 a          845 c         1349 g         1775 t
ORIGIN      
        1 aagacatgga tctttgcact agggcaggcc ttcttctcct tatccgtcgc aggaaatgga
       61 acattgatct atggatcgta tttatccgat gaagaagaca tcccatcatc tgcggcaaga
      121 gttgcatttt ttgatacggt tgctgcgatg cttgcagcac tcgtgatcat cccagctatg
      181 gcaacaactg gagcaacact ggatcaagga ggaccggggc tgttatttat ctatcttcca
      241 aacctgatca gctccatgcc aggaagtacg atcatcgcga tcatcttttt cgttgcagtg
      301 ctttttgcgg gaatgacatc actgatcaat ctctacgaag caccgatcgc aaccgtacag
      361 gaaaagcttc acgtaggaag aaagaccgcc tgcgtgatta tcgcagtgat tggcgtgatc
      421 gtctccttat tgatccaggg catcgtatct gactggatgg atatcctatc tatttacatc
      481 tgcccactag gagcaggact cgcaggaatc atgtttttct ggatcgccgg aaagaaatat
      541 gtagaaacac aggtaaacaa aggaagagaa acaaaattta caaagatgta ttatccaatc
      601 tgcaaatata tttatgtacc gatctgtatt ttggtattgg tgcttgggat tgcgttaggc
      661 ggaattgggt aatgaatgaa ggatcgtagt taaggtatat cttggctgcg attttttctt
      721 atatgaaagc aaacaaatta agttccaaga aaccatacga aattgcaagg aaaggatatt
      781 tacatttaag aagatttttg ttatgatata actatcaaag tagaagtatt attgaatgcg
      841 atatgactat aaatatagaa taaataacaa agataaaggg gatttagcta tggaattaaa
      901 agaattaaca agtgcagaac aggttgtgat gaaatgtatc tgggattatg gaaaggatat
      961 gccattacag cagttgattg aaaatctaaa gcaagattac ggaaaagatt ataaaagaac
     1021 aacgatccgt acatttgtcc ttcatttaga agagaaagga tatgtgacaa catatcagat
     1081 tgggaaatat tcttatatta attatctgat cgaagagcag gattataaag aacaggaatt
     1141 ggaaaagaca aagaatttct ggtacaaaga atctggattt gatctggtga agacattata
     1201 taaaggaaaa ttaaatgagg ggaaactaaa gaaactcaaa aagttgttag aagaaatcga
     1261 tgattgacag gagaagatat gatagacata agagagatat tggttaggat atttagtgcg
     1321 atatttttat ccatgtgcac tggaagctgt atgtttgttt tctggatggt actaagaaag
     1381 ttttttgcag acaagattcg tccaaaagtc tatgatttga tattaaagat cattctaatt
     1441 gcttactatg ttccggcagg atatctgttg gtaaatattt tttttgataa tgggtatgta
     1501 tttgatttta caggtactat catcaatgct ttttatgcta tatcgttgtt ctggctggct
     1561 ggagcaattg caacagttct gaagtttgga gaacgaactt tccgaattag aagagaaaaa
     1621 gaaagatgct ttccatgtaa gatgtatgtg caaaagatct ttgaagattg taaaagagag
     1681 cttggaattc gcagatcaat agaagtatta caaggatatc gcattcagat tccaatgaca
     1741 gcaggaatat taaaaccatg tgtgttttta cctgtggaag atatggaaga agaacaatta
     1801 aaaacgtgta tttaccatga actcacacat tataagaagc acgatatatt ttggaattat
     1861 atagcatgtc tgatggtatg tattcactgg tattgcccat ggatcagaac tgtatttcgt
     1921 aagaacgatg aatggagcga ggtgatttgt gatctatcag cgattggtta tgtaggatca
     1981 gcgaagcgtt attttacaac gatttttgag atgagtcaga aaagtcaggg aatcaaagca
     2041 tatcgggcag catgtctgtt tgaaagccga gatagtttag aacaaagaat ctactatgca
     2101 atgatgtatc gaaaacaaaa gaagataaaa tatgctgcgg tgattgcaat gaccgtgata
     2161 ttttgtggaa tgagtgtaac cagtattttg gctgcaagta agggatatca aaaggcgtat
     2221 acaaaatggg tacaagaaac ggaaattgaa atagaagaac cagatgatga ggaagaggag
     2281 agaatatcgt atgaagaaaa aacgggagtt ttgggaaatg ataagagtga aacgataaag
     2341 aagataaatt ttaaaaagat ggatgcgatg acattggaga cttatgtaaa ggcaggaaaa
     2401 aggcttcgat ataaaggatt aactccgaaa cgaaatgaaa atatagaaat ttttataaca
     2461 agtcaaaaaa attttaaggg gataaaagtt gggattattg attcacagaa taggattaga
     2521 tacatacagg acagagggag agatttagtt gaacatagat ttaagataga aaaagagggg
     2581 aaatatgatg tgtttattga aatggaggat aagaaagatg taaaaattgt tggaatgatt
     2641 aatattgatg atgattaaag agatggagga ataggaggag aaaacgatga taaaatatat
     2701 aaaaataatg gtaacagtat ttacactttt aattagttgt tcagtgacaa atatatttgc
     2761 aagtgatacg aatgatgtta tagaagaaaa gatagatata tcagaaaata ttattgatga
     2821 agttggagag gaagactttg aactgttgtc taattcgata acagttaatt ggacagtgaa
     2881 aaatggagtt agaaagaaaa ctaaagaatt ttataaaaag aaaggaagta gtatcaatat
     2941 taggttacag atagctccaa aaacaaaaag tgtcaaagta ggatatttag atgcagataa
     3001 agtaaaacat tataaaactg ttaaagcaga aatcaatcac aattttataa tacaaaaaac
     3061 aggatatata gaggtgtttg ttgaaaatag tagtggaaaa actgtaacgg caagtggaac
     3121 ttatactaag taatatttta tttataaatg tattttataa gaggtgatgc aatatgaaca
     3181 attaaaaaat ccatgtaata cgaatggctg ccacctacct atctgtaata tatacagata
     3241 gctgtatcac atggatacaa taatgatagc agaaaatgag ggaaaataaa agaatgaaag
     3301 gaaaaagttt atgaaattaa aacaatttgt atgtaaagca gcaagtattg caatgtgtgc
     3361 tatgattatt ggaacaactg tagtttcagt gaatgttaag gcagatacga aagcaacaga
     3421 aagtacagta gcactagata cacatgatga cgatggtgtt gcgggtattt tggaacaaga
     3481 tgattttgat acactagaag aatatcagaa atatttagag acacacccaa aagtacagac
     3541 acggcaaagt cgtgtatcag cgaataaaaa tgtaaaagca gcagctactt taagatataa
     3601 aattaaagga ttaacaaata cagctgcaat tcaaaaaaca tatattggat ctacatatnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngattcaga gaattggctc agactcacgt
     3721 ttatcaagat gtttgattaa tgggtcaaca gctacatatc aagatcatat gacattaaaa
     3781 aactttggtc atggacagac tttggaatgg tttgaacata attctaaagc atatttctgg
     3841 gttacatgta aagcaaatga agcgtataaa tttaaatggg gaactcaaat tggaagaata
     3901 caatatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
     3961 attatacaga aatcccaaga ttttctcata tgagttatgc aaataaaagt gggacatcga
     4021 ttggagaagt gaaacgtgta gatgcagcat tgtctagtga taggaaaaaa gtatttttct
     4081 gggtaatgga taatacagga gaaattcaat atagttttta taatgcggaa aaattaaatg
     4141 ctgaattgga taaaaaagaa tctgaagaat ctaaatttgt tccatgtaca tcaagtgcag
     4201 taaaatcagc ttgttatgga tcgtttagac aatctggaag taatcgtgta ttaccgaatg
     4261 attcatgtca gggattagaa ttttctgatg gagattctat ttatattatt ggaggtgctg
     4321 ccggacaaaa accgggaata gcaaagttaa caggaagtgg atcaagttat aaatattctt
     4381 gccttgttac agctacacat aataattttg gaggtaatgc ggaatctgaa ggaattcaat
     4441 taaagggaga ttacgtatat ttcgggatta gtgataaaca aagtagcgat aaagcctgta
     4501 tttattctat tccaaaaagt gcgttttaat ttaagtctgg ctatgttaaa atatataata
     4561 taaacattaa taaattaaaa ttataaggag gtagcaagat ggtaagaaaa cgtatggtat
     4621 caacagtcat gagcttaatg atggcagcag cagtactgac gacagttcca gtgactaaca
     4681 atgtaaaggc agcagataaa gagataacaa gtggggatta tacttatgtg aaagaaagca
     4741 atggaaagac ttcctatgca gtgcttacaa gttataaagg aagtgagaca aatcttgtca
     4801 ttccagaaga actggatgga ttacaggtaa aagcaatttc acagggattt gaaaaaaatc
     4861 tgaaaataaa aagtattata ttatcaaaaa atattgcact agcaaaagaa acacacagag
     4921 atttggaagt gctaaatgaa attgaaacat tggaagagat tcgtgtagca aaagataatc
     4981 tgtcttatca ggcacaagat ggagttttat atagcaaaga taagaaacaa ttgtttagct
     5041 atccaaaatc taaaaagtcc gaaacatata acatgcctgc ttctgtaaaa aaagtagaag
     5101 aatccaatgc cctaacaaat cttaaatatt taaagaatct tacattatca aagaatttat
     5161 cagtaacacc aagctgtaat gacagtagta ttgaatctgt aacaattccg ggccagatag
     5221 gcggtattga tgaaagcagt tttgaaaatt gcaacaaatt aaataaagtt acaattacaa
     5281 aaggtctgag atttattgat gattatgcat tttttgaatg taaagcatta aaagaaatca
     5341 aactgccaga aggacttcaa agtattggag taggagtatt ttatagaaca ggaattaaac
     5401 agctgacaat ccctggatct gttgtaaaaa ttgatgtaat tgacaagagt ataaaattaa
     5461 gtaaaccatc ttatttgaag aaattcaaaa gagactcagg tgctatttat tacgaagcaa
     5521 gagccacaat caaagcatct ggcaaaaaag ctgtcacata caaagcatcc agaattacaa
     5581 agattaaagc aaaaacaagc aaagtaacga tccaaaaagg aaagacaaca aaactacaga
     5641 ctagagtata catttccaag aaactgaaaa aaggatactt agatccagag atcttaaaat
     5701 tcacaacatc caataaaaaa gtagtgaaag tatcctcaaa aggaacgatc aaaggcttga
     5761 agaaaggaaa agcaacggtc acagtaaaac ttcgtacaac aggaaagaca tataaagtga
     5821 acgtgaaagt aaaataggaa acaataaagt aaagatacat gggtagaaca ggacattccg
     5881 gcgaaagcag ggatgtcctg tgttttctta tgcagcattc cttgttattt gcaatatcaa
     5941 atggtagaat taggaatatt caaagtgaat caatctgtaa tatggaatgg aaaacaatcc
     6001 gatacaacaa aggagaaacg aaacaatcat gccaggatac attttacatt taactgctgc
     6061 ccagatgttt ttaaaaacac aaaaaggaca ggaattttta aagaccaaac aagataaaaa
     6121 tgattttctg atcggaaatc ttctgccaga tacaacaaag atcaaagcaa gatcccattt
     6181 cagagatcca aaatatcacg accgcatgat agaatacccg gaaacaagct ggtttatcaa
     6241 gaaatataag catcttctat caaacagcag tgtggtcgga tatctctttc atctctacat
     6301 cgatcgaaga tttttcaaag actatatgcc aaggatcgta gagtttcgaa atgtacaaga
     6361 tgagagagaa gaaagaagag atatggtaaa agatgtttta ttaaaacgga caggacaaag
     6421 attatcaaag tgggattttt tctcagagaa atactactat ggggactaca caaagatgaa
     6481 catgtacctt gtaaatcgtt atcagattcc gacaacactt gatcccaata tatcaaatcc
     6541 tgggatcaaa gaagttgatt atgaggatgt aaagcaggtg t
//
LOCUS       DAOMUE010000091         6576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3143_length_6576_cov_2.572765, whole genome shotgun sequence.
ACCESSION   DAOMUE010000091 DAOMUE010000000
VERSION     DAOMUE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3143_length_6576_cov_2.572765"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLC71_05395"
     CDS             <1..297
                     /locus_tag="PLC71_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRM02109.1"
                     /translation="MIYHTDTPTNVLPSIHVFNSLACCVAILKNQRKYKKSTDTACVV
                     LTILIIMSTMFLKQHSVVDVVLAALMYTGCYRIIYGNELWSQNAFRTSIFSDHS"
     gene            372..1304
                     /locus_tag="PLC71_05400"
     CDS             372..1304
                     /locus_tag="PLC71_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HRM02110.1"
                     /translation="MRKKIWMILVCIISMSFCMAGCTGNTSKKKSNGKIRITTSFYTM
                     YDFAKKIGGDKVEVINLVPAGTEPHDWEPSTKDLIEMEKSDIFIYNGAGMEQWVDDVL
                     ESLDTEELTSVEASKGIKLLKDQDAHEHDHEHNSENDPHVWLDPQNAKYEMNKIKKAL
                     IKVDAENKDYYEANYKRYAKECDKLDTLYKEETSKLTKKELVVAHEAFGYLCHAYGLE
                     QMGIEGLSADDEPDPKQMSEVIRFAKEHQVKTIFFEELVSPKVAKTIAKETGANAKML
                     NPLEGLSNKKIKAGEDYFSVMKQNLAAIKEALSE"
     gene            1301..1975
                     /locus_tag="PLC71_05405"
     CDS             1301..1975
                     /locus_tag="PLC71_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRM02111.1"
                     /translation="MSESNTKQMIQLNHVKFGYSDTEVLEEINLTIHEGEYVVLTGEN
                     GSGKSTLMRLILGEMKPDEGEIILMNNDPRLKKRIGYVSQNGISKNQSFPATVEEIMI
                     TGLYQELGLFHLPHKKHKNKIKETLRDFGMEEFLKRRIGELSGGQQQRVMIARAMIGD
                     PKLLILDEPTTGVDARSTKILYDMLYEINTKYNTTIFMITHGRLQECKGCDRILRMDE
                     GRIVEE"
     gene            1972..2772
                     /locus_tag="PLC71_05410"
     CDS             1972..2772
                     /locus_tag="PLC71_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRM02112.1"
                     /translation="MIEMLQYGFMQRAFITGILLAVIAPLIGITIVLKRMSMIGDALS
                     HASLAGVALGLLLGINPILGAMGICLFAALGVEAIRRKIPRYSEMAISIIMSTGIGLA
                     GILSGFVKNGTNFNSFLFGSIISISKDELKIVCVISVLVLLCVLLLYKELFYIGFDEN
                     AARISGVPVKTINFIFTLLTALTVSIASRTVGTLIVSSMLVIPIACGMQVGRSYKAVM
                     IIGVITALVTTVIGLTLSYYVGLKPGGTIVLLEVVCFLILALISRYTK"
     gene            2884..4323
                     /locus_tag="PLC71_05415"
     CDS             2884..4323
                     /locus_tag="PLC71_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HRM02113.1"
                     /translation="MIVQLIENIYQFLWGEWIHVPLPGGGSLGLSLLIILLLPAGIVF
                     TIKTRFLPIRLFPDMVKALTANNKNGKDEKSSLSSLQTLIVSTATRVGMGNLTGVVAA
                     ISAGGAGAVFWMWITALLGSSTAFIEATLAQLHKEKDPLYGGYRGGPAYYIHHFMEER
                     QGKKKTHTLIAVLFAISGLICWCGISQVVSNSVAESFHNAFHIPTLYTTIVLVIVAAV
                     IVLRKNATVKVLDIVVPIMAVIYFGITIFVILTNLPSIPGVFARIFKEAFGIRQVAAG
                     GFGAVLMNGVKRGLFSNEAGSGSAPCAAAAADCERPAQMGLVQALGVFIDTIVICSCT
                     AMLMLLAPQNLTDGLTGMNLLQTAMNYHLGGFGVVFIAVILWMFSFSTFLGILFYARS
                     NVAYLFGDKWGWQTAYKVLALVMLFIGGIQTYTFVWDLGDVGIGLMTIFNIFILYAMS
                     GQAIKELKNYEETKKKSIEERAYALQEDE"
     gene            complement(4491..>5010)
                     /locus_tag="PLC71_05420"
     CDS             complement(4491..>5010)
                     /locus_tag="PLC71_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM02114.1"
                     /translation="GSAVELNYEYFAYYLVNAFAQTVVTFTGQNYGAKDEERCKKIFR
                     LGMLCSVISCGILSTIFVVFRTFFIGLFTSDPSVYHYAQLRFLIVLTFECLTSSYEIS
                     GGCLRGFGRSMTPAILTVFGSCVLRLIWLAPVCNWFHDYKLLMAIYPISWVLTGTMVL
                     VAYFRTRKKLFL"
     assembly_gap    5011..5020
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5021..5845)
                     /locus_tag="PLC71_05425"
     CDS             complement(<5021..5845)
                     /locus_tag="PLC71_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM02115.1"
                     /translation="MKNSSKQSVKMDMLHGSLFDKMLMFAMPLAACSILQQLFNSVGT
                     AVVGRFASSEALAAVGSNSSVIALMVTLFSGISLGSNVVIANYIGQNDTKRIPRVVHT
                     AVSLALLSGVFLLILGQFVAHPILLLMGAPKDIIHLATLYLRIYFLGMPFFMLYDFGA
                     SILRSIGDTRRPMYALIVSGVVNVILNLLFVVVFHMGVAGAGLATVGANATSAVQILY
                     FLTHEELPIRLSFKSLTIDRDAVSKILKIGVPAGLQGMVFSLANVCIQSGINSFGAG"
     gene            6032..>6576
                     /locus_tag="PLC71_05430"
     CDS             6032..>6576
                     /locus_tag="PLC71_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM02116.1"
                     /translation="MQISILLLKQIVQLFLMILMGYIMVKIKFLKEEDSKVISKIILY
                     LIVPSVIITAFQVDYTPSIMKGLAVACIASVILQFLLLFVTWIMGKMFHLNTVEYTSA
                     YYSNSGNLIVPLVTYILGKEWVIYGCVFMSVQLFFIWTHCKCKISGETEISLKKILLN
                     INMISVFAGVFLFFTKIRFPE"
BASE COUNT         2275 a         1268 c         1392 g         1631 t
ORIGIN      
        1 atgatctatc acacggatac tccgacgaat gtacttccaa gtatccatgt atttaactct
       61 ctggcatgct gcgttgcgat tttgaagaat cagagaaaat ataagaaaag caccgataca
      121 gcatgtgttg tcttaacgat attaattatc atgtcgacga tgttcttaaa acaacacagt
      181 gtggtggacg tagtgctcgc agccctgatg tatactggat gttacaggat catctatggg
      241 aatgaattat ggtcacagaa tgcatttcgc acgtctattt tcagcgatca ttcgtaaacc
      301 acgcttgaca cgaccaagaa agatttgcta taaataccag agagaaaccg cagaaattgg
      361 agtagagaag aatgagaaaa aagatttgga tgatcctggt atgcatcata tccatgagtt
      421 tttgcatggc aggatgtact ggcaatacaa gcaagaagaa atcgaatggc aagataagaa
      481 ttacaacaag tttttacacc atgtatgatt ttgcaaagaa gatcggtgga gacaaagtag
      541 aagtgatcaa tttagtacca gcaggaacag aacctcatga ctgggaacca tcaacgaagg
      601 atctgatcga gatggagaaa tcagacatct ttatttataa tggagccggc atggaacaat
      661 gggtagatga cgttttggaa agccttgata cggaagaact tacaagcgtt gaagcatcca
      721 aagggatcaa gttactaaaa gatcaggatg cacatgaaca cgatcacgaa cacaacagtg
      781 agaatgatcc ccatgtctgg ttagatccac agaatgcaaa gtatgagatg aacaagatca
      841 agaaagcatt gatcaaagtg gatgcagaga ataaagatta ttacgaagcg aattataaga
      901 gatacgcaaa agaatgtgac aaattagata cattatataa ggaagaaaca tccaaactta
      961 caaagaaaga acttgttgtg gcacatgaag catttggcta tctatgtcac gcatatgggt
     1021 tagaacagat gggaattgag ggattatccg cagatgatga accagatccc aaacagatga
     1081 gcgaagtgat ccgctttgca aaagaacatc aggtaaaaac aatctttttt gaagaactgg
     1141 tgagtccgaa agtagcgaaa accattgcaa aagagaccgg agccaatgca aagatgttaa
     1201 accccctgga aggactaagt aacaaaaaaa tcaaagcagg agaagattat ttttccgtaa
     1261 tgaaacaaaa tctcgctgcg atcaaagagg cattaagcga atgagtgaat caaacacaaa
     1321 acagatgata caattaaatc atgtaaaatt cggatacagt gatacggaag tcttagaaga
     1381 gatcaatctg acgatccatg aaggcgaata tgtcgttctg actggtgaaa atggaagcgg
     1441 aaaaagtaca cttatgcgtc tgatcttagg tgaaatgaaa ccagatgaag gagagatcat
     1501 cctgatgaat aacgatccaa gattaaagaa acggatcggt tacgtgtcac agaatgggat
     1561 cagtaagaac cagagcttcc cagcaacggt agaagagatc atgatcacag gactttatca
     1621 ggaacttggc ttgtttcatc tgccacataa aaaacataag aataagatca aagagacact
     1681 aagggatttt ggaatggaag aattccttaa gagacggatc ggagaattat ctggtggaca
     1741 gcagcagcga gtgatgatcg caagagcaat gatcggagat ccgaaacttc tgatcttaga
     1801 tgaaccaaca acgggagtag atgcaagatc aacaaagatc ttgtacgata tgctctatga
     1861 gatcaataca aaatacaata cgaccatttt tatgatcaca cacggaagac tgcaggaatg
     1921 taaaggatgt gatcgtattt taagaatgga tgaagggagg atcgtggaag aatgatcgag
     1981 atgttacaat acggatttat gcagcgtgca tttattacag gaatcttact ggccgtgatc
     2041 gcaccattga tcggaatcac gatcgtctta aagagaatgt ccatgatcgg agatgcactc
     2101 tctcatgcat ccctggcagg tgttgctctg ggcttgttat tagggatcaa tccaatctta
     2161 ggagcaatgg ggatctgcct gtttgcagcc ctgggagtcg aagcgatccg aagaaagatc
     2221 ccaagatatt ctgagatggc gatatcgatc attatgtcaa cagggatcgg acttgctggt
     2281 atcctatccg gattcgtaaa aaacggaacg aactttaaca gtttcttatt tggaagtatc
     2341 atatccatca gtaaagacga actgaagatc gtatgtgtga tcagtgtgtt agtattgctc
     2401 tgtgtattgc ttctatataa agaactattc tatatcggat ttgatgaaaa tgcagcaagg
     2461 atcagcggtg tacctgtcaa gacgatcaat tttatcttta ctttattgac cgcattgacg
     2521 gtatcgatcg catcaagaac ggttggaaca ctgatcgtct catccatgct tgtgatcccg
     2581 atcgcatgcg gaatgcaggt aggacgaagc tacaaagcag tcatgatcat cggtgtgatc
     2641 acagcattgg tcacaacagt gatcggatta acactgtctt attatgtagg tcttaagcca
     2701 ggaggaacga tcgtactgct cgaggtggta tgtttcttga tccttgcact gataagcagg
     2761 tatacgaagt aaattttttg acatttatca gaaaatttta tataataaaa agaaggcatg
     2821 cataacagaa taaaactgca tgccttcttt tttggaaaaa acaatgaaag gaaaggcaag
     2881 tcaatgattg ttcagctaat agaaaatata tatcagtttt tatggggaga atggattcat
     2941 gttccgttac caggaggtgg aagcctggga ctttcgctgc tcattatatt gctgcttcca
     3001 gcaggtatcg tttttacgat caaaacaaga tttttaccga tccgtctgtt cccggatatg
     3061 gtcaaagcac taacagcaaa caataagaat ggaaaagatg aaaaaagcag tctttcttct
     3121 ttacagacct tgatcgtatc aacagcaaca cgtgtcggaa tggggaatct gacaggagtt
     3181 gtcgcagcaa tatccgcagg tggagcaggg gctgtattct ggatgtggat cacagcattg
     3241 cttggatcat cgacagcctt tatcgaagca actcttgcac agttacataa agaaaaagac
     3301 cccctgtatg gtggatatcg aggaggacca gcctattata tacaccactt tatggaagaa
     3361 agacaaggaa agaagaaaac acacacactg atcgctgtct tatttgcgat ttcaggattg
     3421 atctgctggt gtgggatcag tcaggtggta agtaattccg tggcagaatc attccacaat
     3481 gccttccata ttccaacact ttatacaacg atcgtgcttg ttattgtggc agcagtgatc
     3541 gtactaagga agaatgcaac ggtcaaagta ttagatatcg tggtaccgat catggcagtc
     3601 atttacttcg ggattacgat ttttgtgatc ctcacgaatc tgccaagcat tccaggggtt
     3661 tttgcaagaa tcttcaaaga agcctttgga atccgtcagg tcgcagccgg aggatttgga
     3721 gcagttctta tgaacggagt gaaacgagga ttattctcca acgaagcagg atcaggatcc
     3781 gcaccatgtg cagccgcagc agcagactgt gaacgcccag cacagatggg attggtacag
     3841 gcattaggag tcttcatcga cacgatcgtg atctgcagct gtacggcaat gttaatgtta
     3901 ttagcaccac agaatctgac tgatggatta actggtatga acttgttaca gacagcaatg
     3961 aactaccatt taggaggatt cggagttgta tttatcgcag tgatcctctg gatgttcagc
     4021 ttctcgacat tccttggaat cttattctat gcaagatcca acgtcgccta cttattcggt
     4081 gacaaatggg gatggcagac agcctataaa gtattagcac ttgtgatgtt atttatcgga
     4141 ggaatccaga cctatacatt cgtatgggat ttaggagatg tcggaattgg gttaatgacg
     4201 atctttaata tctttatcct gtatgcaatg tcaggacagg cgatcaaaga gttaaagaac
     4261 tatgaagaaa caaagaagaa atcgatcgaa gaaagagcat atgcattgca ggaagatgaa
     4321 taggaagtaa ataagtgaaa taaaaccgaa caattcacgt aaatatgatt tcaatttacg
     4381 taaattgttc ggtttttttg tgttaaatat taaaaatagc aaagaaaaat gtctgttaga
     4441 tctattatga aacaaatagg tataacagac attttaatta catcatcaaa ttataaaaac
     4501 agcttcttac gtgtcctaaa ataagccacc agaaccatcg ttccagtcaa tacccaagag
     4561 atcggataga tcgccatcaa aagcttataa tcatggaacc agttacagac cggtgcaagc
     4621 cagatcaaac gtaatacaca agatccaaac actgtcagga tcgcaggagt catggaacgt
     4681 ccaaatccac gcagacagcc accactgatt tcataagaac ttgtcagaca ctcgaatgtt
     4741 agaacaatca aaaaacgaag ctgagcataa tgatagacag atggatcact tgtaaataat
     4801 ccaataaaga acgtacggaa tacaacaaag atcgtactaa ggattccaca agagatcaca
     4861 ctgcacaaca ttccaagacg aaaaatcttc ttacaacgtt cctcatcctt cgcaccataa
     4921 ttctgtccgg taaatgtaac taccgtctga gcaaaagcat tgaccagata ataagcaaaa
     4981 tattcataat ttaattccac cgcagaaccc nnnnnnnnnn gccagctcca aaactgttga
     5041 tcccagactg gatacagaca ttggcaagag agaaaaccat tccctgaagt cccgcaggaa
     5101 ccccaatctt caggatctta gaaacagcat cccgatcaat cgtcaaagac ttaaaagaca
     5161 agcggatcgg aagttcttca tgcgtcagaa aataaagaat ctgaaccgca cttgttgcat
     5221 tcgcaccaac cgtcgcaagt ccggcacccg caacacccat atgaaataca acgacaaata
     5281 gaagattcaa gatcacattc acaacaccag acacgatcaa agcatacatc ggacgacgtg
     5341 tatctccgat actgcgaaga atggaagcac cgaagtcata taacataaaa aatggcattc
     5401 caagaaaata aatccgcaga tataacgttg caagatgaat gatatccttc ggtgcaccca
     5461 tcagcaaaag gatcggatga gccacaaact gtccaagaat cagcaaaaac acaccactaa
     5521 gcagtgcaag agacacagcg gtatgtacca cacgaggaat ccgcttcgta tcattctgtc
     5581 cgatataatt ggcgatcaca acattagaac caagagaaat cccagaaaac aatgtaacca
     5641 tcaaagcgat cacagaacta ttactaccaa ccgccgctaa agcctcgctg ctcgcaaacc
     5701 gtccgacaac ggcagtaccg accgaattaa aaagctgctg taagatacta caggcagcaa
     5761 gcggcatagc aaacattaac atcttatcga ataaacttcc atgaagcata tccatcttca
     5821 cggactgttt cgaagaattc ttcatataca ataccctctt tcaaaaaact atacagattc
     5881 taatactttt tgggaagatt atcaagatga ttccaggaaa aacaacgaag aaagatgtat
     5941 caaattcgga acatttcttg tataaaaaaa gcagaatgat atactaggat gagacattaa
     6001 gaaaacacac gaaagaaaga aggaaaacac catgcaaatc agcattctgc tgctaaaaca
     6061 gatcgtacaa ctattcctga tgatcctcat gggttacatt atggtaaaga tcaaattttt
     6121 aaaagaagaa gacagcaaag taatctcaaa gatcatactc tatctgatcg taccatctgt
     6181 gatcatcacc gcatttcagg tagactatac cccaagcatt atgaaaggac tggcagttgc
     6241 atgtatcgca tctgtgatcc tgcagttttt attactgttt gtcacatgga tcatgggaaa
     6301 aatgtttcat ttaaacacgg tggaatacac atctgcctac tattccaact caggaaacct
     6361 gatcgtacca ttagtcacct atatcttagg aaaagaatgg gtcatctacg gctgtgtctt
     6421 catgagtgtt caattattct tcatctggac ccactgcaaa tgtaagatca gtggagaaac
     6481 agaaatcagt ttaaagaaga tcctgttaaa tataaacatg atctctgtat tcgcaggagt
     6541 attcttattc ttcacgaaga tcagatttcc agaaat
//
LOCUS       DAOMUE010000092         6562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3152_length_6562_cov_2.109421, whole genome shotgun sequence.
ACCESSION   DAOMUE010000092 DAOMUE010000000
VERSION     DAOMUE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6562
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3152_length_6562_cov_2.109421"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..912
                     /locus_tag="PLC71_05435"
     CDS             274..912
                     /locus_tag="PLC71_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009264838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRM02117.1"
                     /translation="MFLNEATKLRAETIIKGLEKRNMHGVFCATKEDALKQALSYIKE
                     GSSVSWGGSMSVSEIGLMDALKEGNYHLIDRSVAKNFDEQREIFSKAVLSDYFLMSSN
                     AITLDGELINIDGTGNRVACLSYGPKHVIMIVGMNKVVNDVEDGIKRVRNFASPPNTL
                     RLGLKTPCSMTGRCGDCYGDTCICSQIVVTRRQSAMMKDRVKVILVGESLGY"
     gene            1107..2045
                     /locus_tag="PLC71_05440"
     CDS             1107..2045
                     /locus_tag="PLC71_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM02118.1"
                     /translation="MNYDIYNSSITYFPLLPLSKTVSFERKVLDIRQMPAQVLFPHWD
                     DEFEFIYVLSGMMEFRVRQKAFLVSSGEGFFLNTGEIHSACAYQSYDCRFVIYRICPS
                     VLFQTEDNPLYQKYIKPLVDHPSFGFLTFVPKVPWQKYILEMIRKMNDICDRPVDGYE
                     LKINHFVNEIFYYIFHNVNLSKELSLKESRDLERMKDVMIYLTKTIDQKHTLADIASY
                     CHLSNSECCRLFQRNVHLSPVDYLNQLRIHMSLSEIIKHEKKITDIAKMYGFSGSSYF
                     SEMFKKTLGITPRAFYKSVLQQQTLSSNADPIHGRR"
     gene            complement(2014..2403)
                     /locus_tag="PLC71_05445"
     CDS             complement(2014..2403)
                     /locus_tag="PLC71_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02119.1"
                     /translation="MRNQYEKQEKVLWYAQIMIFLGVLATWFNHMDMHTFYTINIILS
                     VISIISLTLDRFYFSEKIGQKVHNSYSVAYGLLVIFTLLLVILYAGILKIDLIMQEGT
                     KMIWALTPALSLLWKTKFIAFREWDQR"
     gene            complement(2429..3919)
                     /locus_tag="PLC71_05450"
     CDS             complement(2429..3919)
                     /locus_tag="PLC71_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRM02120.1"
                     /translation="MRITKKQGGIIGGAVAVVLIAAAIGVHAHYQNRWYPGSTFNKVD
                     VSGMTYEESVKKVKKSIDSYKLKIKGRNNGQKVISGKEIDLAFKTESHVKDAYKKQHS
                     QSVFSTIFGGKKNKVTAVALSEQKLKAKLKQSVLIKGSDTYKITKPVDATIVYSADKK
                     YGVIQKEDEGNYLNRKAFYDAVEKSIESLSNTLNLTDEKKNPDVYKKPGLYHDDEELK
                     QMQTTYNEYLLHFIQWDMGNNVKETLGPDVLKDCIKVNTKRHTVKLDQPAVEKWLESF
                     CLKYKTQGIARTFKTHSGKKIKVSGGDYGWRIDYDKVIEQTMKALKKAPDESAIKAYE
                     KDPSKENEQALLTSLKPVYSHKGYRMDYTNKQNDWDTQNYSEVDLSAQEVFVYKKGKL
                     VFSTTCITGKATPDRITRTGVYDIKEKKLTKTLTGADYSVPTRYWTRIMWTGTGYHYS
                     SRGDWGSWSPTYYKTNGSHGCVNLTLSDARSVYNLTKIGDPVFIHY"
     gene            complement(3956..5191)
                     /gene="glyA"
                     /locus_tag="PLC71_05455"
     CDS             complement(3956..5191)
                     /gene="glyA"
                     /locus_tag="PLC71_05455"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRM02121.1"
                     /translation="MFDFSEVKNYDPELAEAMENELTRQRTNLELIASENLVSKAVMA
                     AMGSHLTNKYAEGYPGKRYYGGCQYVDVVEDLARERAKELFGCEYVNVQPHSGAQANM
                     AVFFAVLNVGDTYMGMSLDHGGHLTHGSPVNMSGKNYHCVPYGVNDEGFIDYDEVERI
                     ALECKPKMIIAGASAYARAIDFKRFREIADKVGAVLMVDMAHIAGLVAAGLHQSPIPY
                     AHVTTTTTHKTLRGPRGGMIMCSNEVNEKYNFNKAIFPGIQGGPLMHVIAGKAVAFKE
                     ALDPSFKEYMTQVVKNADTLANALIEEGFDIVSGGTDNHLMLVDLKKYDLTGKEAEKV
                     LDSVHITCNKNTVPNDPKSPFVTSGLRLGTPAVTTRGLKEDDMKVIAKAIRLTLLDKK
                     LDEANELVLGLTEKYPLYE"
     gene            complement(5284..5754)
                     /locus_tag="PLC71_05460"
     CDS             complement(5284..5754)
                     /locus_tag="PLC71_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRM02122.1"
                     /translation="MEKMLFSGVQSNSEVALIAIKGIKNQPGIAAKVFGAIAEEKINV
                     ELILQSIGRGENKDISFVVAQDEADHAVETLKKAFDEADYEDIISDKEVGIVSIVGAG
                     MMSNSGAASKMFEALYNRGINIHLIYTSEIKITVLIKQSRVERAVEALKEQFQE"
     gene            complement(5759..>5944)
                     /locus_tag="PLC71_05465"
     CDS             complement(5759..>5944)
                     /locus_tag="PLC71_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HRM02123.1"
                     /translation="AGLAYGLDRLVMWLTGEDSIRDVIAFPKVKDASCLMTQAPGPVD
                     TLQLEELGIKLSTPEEE"
     assembly_gap    5945..5954
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5955..>6562)
                     /locus_tag="PLC71_05470"
     CDS             complement(<5955..>6562)
                     /locus_tag="PLC71_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HRM02124.1"
                     /translation="RGLNAKGLGDLGRKKIDGFVDYAKDFGAKGLAYIQLKSDGTVKS
                     SFAKFMKEEEMDALIKAMDGQAGDLLLFAADKDKVVFDVLGNLRLHIAKQYDMIDKSQ
                     YKFLWVTEFPLLEWSDEQNRYTAMHHPFTMPFEEDLEFIDERPGDVRAKAYDIVLNGT
                     ELGGGSIRIHQDDIQEKMFEALGFTKEDAHNRFGFLLDAFKFG"
BASE COUNT         1702 a         1370 c         1146 g         2334 t
ORIGIN      
        1 atgtccgacg caccatgtga tgactgcctt gtctccttcc atgtatccgt ctctgttttt
       61 tgcatttata ttcagtgccg ctgcgaattc tcttgcgaca cttggtttct ctgtgataaa
      121 tagtgctttc aaaaaattac ctctttctaa aagttttacg atatattata acataaccat
      181 tgaaaacatg atataattaa atctagttct tgtggaattc atatttgtag aatcatgatt
      241 ccacataaaa tttatactga aaggatgatg agtatgtttt taaatgaagc aaccaaactt
      301 cgtgcagaaa caattatcaa gggacttgaa aaaagaaata tgcatggtgt tttctgtgca
      361 accaaggaag acgccctaaa acaggctctt agttatatca aagagggttc ttctgtttct
      421 tggggtggtt ctatgagtgt ttctgagatt ggactgatgg atgccttaaa ggaaggaaac
      481 tatcatctga ttgatcgttc tgttgcaaag aattttgatg agcagcgtga gatcttttct
      541 aaggctgtct tatctgatta tttcctgatg agctctaatg cgatcacctt agacggagaa
      601 ctgatcaata tcgatggaac tggtaaccgt gttgcgtgct taagttatgg tcctaaacat
      661 gtgatcatga tcgttggtat gaacaaagtt gtcaatgatg tggaagatgg tattaagaga
      721 gttcgtaatt ttgccagccc tccaaacact cttcgtctcg gactgaaaac tccttgttct
      781 atgactggac gttgtggaga ctgttatggt gatacctgca tctgcagtca gatcgttgtg
      841 acaagacgtc agagtgcaat gatgaaagat cgtgtcaaag tgatccttgt tggtgaatct
      901 ctaggttatt aaggcactcg ctctttctat caaatatcac agaacacaga cattttatca
      961 ggcattttca ctggatattt tatcgccatt ccagtgaaaa tgccttttct attcttgaat
     1021 ttttataaca atcatagtaa aatcaaacta tattatgaca caattcgaca ttttttataa
     1081 caatattgat attatgaggt ttttctatga attatgatat ttacaattca tctatcactt
     1141 attttccact gcttccgctt tctaaaaccg tttcatttga acgcaaggta ctggatatcc
     1201 gacagatgcc tgctcaggtt ttgtttcctc attgggatga tgaatttgag tttatatatg
     1261 ttctctctgg catgatggaa tttcgtgtcc ggcagaaggc ttttctggtt tcttctgggg
     1321 aaggtttctt tttaaacact ggggagattc attccgcttg tgcatatcag tcttatgact
     1381 gccgctttgt tatctaccgg atctgccctt ctgttctttt tcagacagaa gataatcctt
     1441 tatatcaaaa atatatcaaa cctctggtcg atcatccttc ctttggattt ttaacgttcg
     1501 ttccaaaagt cccttggcag aaatatatcc ttgaaatgat ccgcaaaatg aatgatatct
     1561 gtgaccgtcc tgttgatggt tatgaattga agatcaacca ttttgtaaac gagatctttt
     1621 attatatctt tcataatgtg aatctctcca aagaactgtc cttaaaagaa tccagagatt
     1681 tggaacgtat gaaagatgtg atgatatatc ttactaaaac gatcgaccaa aagcatacac
     1741 tcgctgatat tgcttcttac tgccatctaa gcaatagtga atgctgccga ctctttcaaa
     1801 gaaatgttca tctaagtcct gtggattatc tgaatcaact tcgtattcat atgagcttgt
     1861 ctgagatcat caaacatgag aaaaagataa cagatatcgc taagatgtat ggtttctctg
     1921 gcagcagtta tttctctgaa atgtttaaaa agaccctcgg cataacgcca agagcctttt
     1981 ataaatctgt tcttcagcaa caaactcttt cttctaacgc tgatcccatt cacggaaggc
     2041 gatgaatttc gtcttccaaa gaaggctcaa tgctggtgtt aatgcccaga tcatctttgt
     2101 cccttcttgc atgatcagat cgatctttaa tattcccgca tacaaaatta ccagtagtaa
     2161 cgtaaagatc actagtaatc catatgcaac agaataagaa ttatgcactt tctgcccgat
     2221 cttctcacta aagtaaaagc gatccagtgt cagagaaatg atcgaaatca ctgataaaat
     2281 gatattgatc gtataaaatg tatgcatatc catatgatta aaccaagttg ccaaaactcc
     2341 tagaaagatc atgatctgtg cataccataa tactttttcc tgtttttcat attgatttct
     2401 cattgattga aacctctttt tctttctttt aataatgaat aaatacagga tctccgatct
     2461 tcgtcaggtt atatacagat cttgcatctg ataatgttaa gttaacacat ccatgagatc
     2521 cattcgtctt ataatacgtt ggtgaccagc ttccccagtc tcctctgctg ctgtaatgat
     2581 atcctgttcc tgtccacatg atccttgtcc agtatcttgt tggaacactg taatccgcac
     2641 ctgttaatgt ctttgtaagt ttcttctctt tgatgtcata aacaccggtt cttgtgatac
     2701 gatctggtgt tgcttttcca gtgatacagg ttgtgctgaa tacaagtttt cctttcttgt
     2761 agacgaaaac ttcctgtgct gacaggtcaa cttcactgta gttttgtgta tcccagtcat
     2821 tttgcttgtt cgtataatcc atacgatatc ctttgtggct gtagactggt tttaagcttg
     2881 taagtaaagc ctgttcattc tccttggaag ggtctttctc ataagctttg atcgcagatt
     2941 catctggtgc tttctttaac gctttcattg tctgttctat aactttgtca taatcgatac
     3001 gccatccata gtctccacca ctgactttga tcttcttgcc agaatgtgtc ttaaatgttc
     3061 ttgcgattcc ctgtgtcttg tattttaagc aaaagctttc taaccatttc tcgacggcag
     3121 gctgatctaa ttttacagta tgtctctttg tgtttacttt gatgcagtct tttaacacat
     3181 ctggacctaa tgtttctttg acattgttgc ccatatccca ctgaatgaaa tgtaataaat
     3241 actcattgta ggttgtctgc atctgtttta attcttcatc atcatgatac agaccaggct
     3301 ttttatagac gtccggattc ttcttttcat ctgtcagatt cagtgtgtta cttaaagact
     3361 ctatggattt ctctactgcg tcataaaatg ctttacggtt taagtaattt ccttcatctt
     3421 ccttctggat tacaccgtat ttcttatctg cagaatatac gatcgttgca tccactggtt
     3481 ttgtgatctt atatgtatca ctgcctttga tcaaaacgga ttgttttaat ttagccttta
     3541 atttctgctc tgataatgca actgctgtca ctttgttttt tttgccacca aagatcgttg
     3601 aaaatacact ttggctgtgc tgctttttgt aagcatcctt tacatgtgat tctgtcttaa
     3661 atgcaagatc gatctcctta ccggaaatca ctttctgtcc gttatttctt cccttgatct
     3721 ttaacttgta agaatcaatg gatttcttta ctttcttcac tgattcttcg tatgtcattc
     3781 ctgacacatc aaccttgtta aatgtactcc ccggatacca acggttctga taatgggcat
     3841 ggacaccgat agcggctgct attaaaacaa ccgctacggc acctcctatg attccccctt
     3901 gtttctttgt aatcctcata ttgatttctg cctccaaata cgactctgtg atggattatt
     3961 catagagtgg atatttttct gttaatccta atactaattc gtttgcttca tcaagtttct
     4021 tgtctaataa tgtcagacga atagcttttg cgatcacttt catatcgtct tctttcagtc
     4081 cacgagttgt aacagctggt gttccaagac gaagtcctga tgttacgaat ggagattttg
     4141 gatcgtttgg tacagtattc ttattacatg tgatatgtac agaatccagt actttttcag
     4201 cttcttttcc tgtcagatca tatttcttaa gatctactaa cattaagtgg ttgtctgttc
     4261 ctccagatac gatatcgaat ccttcttcga tcagtgcatt tgcaagagta tctgcattct
     4321 tcacaacctg tgtcatatat tctttgaatg atgggtctaa tgcttctttg aatgctacag
     4381 ctttaccagc gatcacatgc attaatggtc caccctggat tcctgggaag attgctttgt
     4441 tgaagttgta tttttcgttc acttcattgc tgcacatgat cattccacca cgaggtcctc
     4501 ttaatgtctt atgtgttgtt gttgttgtta catgagcata agggattgga ctctgatgta
     4561 atcctgcagc tactaatcct gcgatatgag ccatatctac cattaaaact gccccaactt
     4621 tgtcagcgat ctcacggaaa cgtttgaagt cgatcgctct tgcgtatgca cttgcaccag
     4681 caatgatcat ctttggtttg cattctaatg cgattctttc tacttcatca taatcgatga
     4741 atccttcatc attaactccg tatggtacgc agtgatagtt cttaccagac atgttgactg
     4801 ggcttccgtg tgttaaatgt ccaccgtggt ctaagctcat tcccatgtat gtatctccga
     4861 cattcaatac tgcgaagaat actgccatgt ttgcctgtgc tccagagtgt ggctgtacat
     4921 ttacatattc gcatccaaat aattctttgg cacgttctct tgcaagatct tctaccacat
     4981 ctacatactg gcatccacca tagtaacgtt ttcctggata tccctctgca tatttgtttg
     5041 ttaaatggct tcccattgca gccataacag ctttggaaac aaggttctct gatgcgatca
     5101 actcaagatt tgttctctgt cttgttaatt cgttttccat tgcttctgct aattctggat
     5161 cgtagttctt tacttcgcta aaatcaaaca ttgttttctc ctttatatat ttatgtaaaa
     5221 taatattgtg tctcgtatat tgtgtcttat tcttatgatc attcgattgc ctgatctatc
     5281 tgattattcc tggaactgtt cttttaatgc ttctactgca cgttctacgc ggctttgttt
     5341 gatcaaaact gtgatcttga tctctgatgt gtagattaag tgaatgttga ttccacgatt
     5401 atacagtgct tcaaacatct tagaagctgc tccgctgtta gacatcattc cagctccgac
     5461 gatagatacg attccgactt ctttatcact gatgatatct tcgtaatctg cttcatcaaa
     5521 tgctttcttt aatgtttcaa cggcatgatc tgcttcatcc tgtgctacaa cgaaagaaat
     5581 atctttattc tctccacgtc cgatggactg aaggatcagt tccacgttga ttttttcttc
     5641 tgcaatggca ccaaaaactt ttgctgcgat tcctggctgg tttttgattc ctttgattgc
     5701 gatcagtgca acttcagagt tagactgaac accgctaaat aacattttct ccatctgatt
     5761 attcctcctc tggtgttgaa agtttaattc caagttcttc tagctgtaat gtatcaaccg
     5821 gtcctggagc ctgtgtcatc aggcaggaag catcttttac tttcgggaag gcgatgacat
     5881 cacgaatgct gtcttctcct gttaaccaca taacaagacg gtcaagacca taagctaaac
     5941 cagcnnnnnn nnnnccaaat ttgaatgcat ctaataagaa tccaaaacgg ttgtgagcat
     6001 cttcttttgt aaatccaaga gcttcaaaca tcttctcctg aatatcatcc tgatggatac
     6061 ggattgatcc accaccaagt tctgtaccat ttaatacgat atcgtatgct ttcgcacgga
     6121 catctcctgg tctttcatca atgaattcaa gatcttcttc aaatggcatt gtaaatgggt
     6181 gatgcattgc tgtgtaacga ttctgttcat cgctccactc aagtaatggg aattctgtaa
     6241 cccataagaa cttatactgg gatttgtcga tcatatcgta ctgtttcgca atgtgaagtc
     6301 ttaagttacc aagcacatca aagacaactt tgtctttgtc tgcggcaaat agtaataagt
     6361 ctcctgcctg tccatccatt gctttgatca gtgcatccat ctcttcttct ttcatgaatt
     6421 tagcgaaaga tgatttcact gttccatcgc tctttaactg aatatatgca agtcctttcg
     6481 ctccgaagtc tttggcgtag tcaacgaatc catcgatctt cttacgtcca agatctccta
     6541 aacctttagc gtttaatcct ct
//
LOCUS       DAOMUE010000093         6550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3161_length_6550_cov_1.872671, whole genome shotgun sequence.
ACCESSION   DAOMUE010000093 DAOMUE010000000
VERSION     DAOMUE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3161_length_6550_cov_1.872671"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLC71_05475"
     CDS             <1..285
                     /locus_tag="PLC71_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM02125.1"
                     /translation="KEQDSEVLSAIACHTTGKIGMTPLEQIVFIADYMEPNRKMIPGL
                     SKVRKLAFEDLDKCTKTILKNTIEYLTECGQEIDERTVETYKYYRDKKEN"
     gene            287..640
                     /gene="rsfS"
                     /locus_tag="PLC71_05480"
     CDS             287..640
                     /gene="rsfS"
                     /locus_tag="PLC71_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRM02126.1"
                     /translation="MDQSLNMVKIAYDALDDKLAEDIKIIDIRSISVLADYFIIADGN
                     NKNQVQAMVDNVQEELFKAGYEMKQMEGYREGNWILLDFGDIIIHIFDKENRLFYDLE
                     RIWKDGKEVSIEELN"
     gene            complement(706..1323)
                     /gene="lexA"
                     /locus_tag="PLC71_05485"
     CDS             complement(706..1323)
                     /gene="lexA"
                     /locus_tag="PLC71_05485"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRM02127.1"
                     /translation="MAKEQLSDKQQQILEFIKRRILDKGYPPAVREICEAVNLRSTSS
                     VHSHLETLERKGYIHRDPTKPRAIEITDDDFNLARREIINIPVVGTVTAGEPILAVEN
                     IEEYFPMLPNHISNKNTFMLHVRGESMINAGIFDGDMVIVEQQPTADNGDIVVAMIED
                     SATVKRFYKEDGYYRLQPENDTMDPIIVSEVSIIGKVVGLYRSMK"
     gene            1499..1789
                     /locus_tag="PLC71_05490"
     CDS             1499..1789
                     /locus_tag="PLC71_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRM02128.1"
                     /translation="MRSKRRVIGIIIAVVFVFLMSTFLLSNNLIEAQGTKDKQFTSIQ
                     VQKGDTLWKIAKKYMGNEYSSVDDYIEEVCQTNHIYDGKITEDMYLVIPYYK"
     gene            complement(1855..3282)
                     /locus_tag="PLC71_05495"
     CDS             complement(1855..3282)
                     /locus_tag="PLC71_05495"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM02129.1"
                     /translation="MRLQKLFITTLLSVSVIGTAIPANVSAASYATTKRTYTLKVAGK
                     KQKKKARGAIYNGKTIKTKAPGFLRGDTTMYSASYVFQKGLGVSYSYSSKTWKITLKK
                     GSKTITMKRGSKYAYVNGKKKKLPTPARRVYSYKQKKNYIYVPGEFCAKHLGYSYSWS
                     SSSYAGTFSTSNSNKASSSVTTLPATNGEHYVQLDKPEGLSESDISTTDDYNNYRLIV
                     NIKGNYSSYYSDASHRSVIGDSSFYSYSVAYSNGYTKIYIRPRKATIKGFEVTQTDSY
                     IKVRYDSPKAMYKQIVVLDAGHGGSDSGAVANGYKEKNMTLKIVQAAKSYFDDDPDIK
                     VYYTRLSDTYPSLTERSDLANEVGADRFYSVHINSAGSSATGTETLYNSKGYKSSTGL
                     TSYNWSATIHPYIRSATGFTNRGLVNRTGLAVLRHTKTSSTLTEIGFISNKSEAKKMN
                     SNLTNYGKAVYNSTKASFSKNPTGR"
     gene            complement(3397..4824)
                     /locus_tag="PLC71_05500"
     CDS             complement(3397..4824)
                     /locus_tag="PLC71_05500"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM02130.1"
                     /translation="MNKKQILCTGLSLALLLSSVITTPASIAAGKYFKIQYIHSKKKV
                     KKKAINARYNNKVISTKIPGYIEGSTSMYSAYWIFGHCSSLGTKYSYSSSKKRVTLQR
                     NSQKLVMTLNSRTATLNGKKFTLPSAPRKIRYIAKKKNYIMVPGDIVAKKLGLNYSWN
                     NRLLSGVISKGSTAKPAPSTPSNTKPQTSNPSGSTTKITASESDYSIRIKKPDGLSSS
                     SISSNDDYWNKQLQIIIDGDYRNFFNTASNRTIKDSLTYKVSYLNGKTYINLITSTIK
                     GFSVTQTDSYIYVKYAAPKDMFYRIIVIDAGHGGKDSGATGNGYIEKNMTLKIVQNIK
                     TNFDSDPLYKVYYTRLSDWYPTLTERYDLANTVNADRFLSVHINSADSASAKGTETLY
                     KDYKTYASVIHSSSLSGMGYTKGSSYDRSLVYRPGLAVLRGTKMMSALAEMGFISNST
                     ESARIDARSEAIGSALYQSLCNSFN"
     gene            5089..5805
                     /locus_tag="PLC71_05505"
     CDS             5089..5805
                     /locus_tag="PLC71_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRM02131.1"
                     /translation="MKSDNEFRRGLIDGIPIGLAYVAVSFAFGISGASKGIPVWALTL
                     ISATCVTSAGQFAAIITMTAGGSLVELILSQIIINLRYSIMSIAIGQKLSEKSTVWNR
                     LSMAFMVTDEIFAVSCAGRHEITPRYFYALMSIPWISWTIGTLLGATANTLLPLILRN
                     ALGLAIYGMLIGIIIPPARQDKHITIVIILSMIVSLLFKYIKALSFISSGFVIIICTL
                     IAATFGAILFPVEEEETHES"
     gene            5795..6106
                     /locus_tag="PLC71_05510"
     CDS             5795..6106
                     /locus_tag="PLC71_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRM02132.1"
                     /translation="MNLSRLLVYIAVTAVSTYLIRVIPLVAIQKKIENRFFRSFLYYV
                     PYAVLAAMIMPAVFYATGNLIASTIGFAIAMIFAYNKKNIVTVACASCIGAFIALVVL
                     G"
     gene            6167..>6550
                     /locus_tag="PLC71_05515"
     CDS             6167..>6550
                     /locus_tag="PLC71_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRM02133.1"
                     /translation="MNCNLAVIKGDGIGPEIVTEAMRVLDAVGEKYGHTFSYTQILMG
                     GASIDVHGEPLTEEALEIAKKSDAVLMGSIGGNTSTSPWYKLPAQLRPEAGLLKLRKS
                     LGLFANIRPAYLYDELKKACPLRDEV"
BASE COUNT         2019 a         1068 c         1361 g         2102 t
ORIGIN      
        1 aaggaacagg attcggaagt tttgtcagca atcgcatgtc atacgacagg gaagatcggt
       61 atgacaccgc ttgaacagat agtttttatt gctgactata tggaaccaaa tcgaaagatg
      121 attccaggac tttcaaaagt ccgcaagctg gcatttgagg atcttgataa atgtacaaaa
      181 acaattctta agaatacgat agagtacctg acagaatgtg gacaggaaat tgatgagcgg
      241 acagtagaga cttataagta ttatagagac aaaaaggaga attaagatgg atcagtcatt
      301 aaatatggta aagatcgcat atgatgcgtt agatgacaag ttagcagaag atatcaagat
      361 cattgatatt cgaagcattt ctgttcttgc agattacttt atcattgcag atggtaataa
      421 caaaaaccag gtacaggcaa tggtggataa tgtacaggaa gaacttttta aagctggata
      481 tgagatgaaa cagatggaag ggtatagaga aggaaactgg atcttacttg actttggtga
      541 tatcatcatc catatctttg ataaagaaaa ccgtttattc tatgatcttg aaagaatctg
      601 gaaagacgga aaagaagtat cgatcgaaga attaaattaa aaagaatgat atgaaaaaag
      661 tcagctatgg gaatgctttg aaagcatctc gcagctgact tctttttatt tcattgaacg
      721 ataaagtcca acgactttac caataataga tacttctgat acgatgattg ggtccatagt
      781 atcattctct ggctggagtc tgtaatatcc atcttcttta taaaatctct taacagttgc
      841 agaatcttcg atcattgcaa cgacaatatc accattatca gcagttggct gttgttcgac
      901 aataaccata tcaccgtcaa aaattccggc attgatcata ctttctccac ggacatggag
      961 catgaaagta ttcttattgc tgatatgatt tggaagcatt ggaaaatatt cttcaatatt
     1021 ctctaccgct aagatcggtt caccggcagt gacggtacct acgacaggga tattgatgat
     1081 ctcgcggcga gccagattaa aatcatcatc ggtgatctcg atcgcacgag gtttggtagg
     1141 atctctatga atgtatcctt ttctctccaa agtctccaga tgggaatgga cagaggaagt
     1201 agaacgaaga ttcactgcct cgcagatctc gcgtacggca ggtggataac ctttatccag
     1261 gatgcgtcgt ttgatgaatt caaggatctg ttgttgtttg tcacttaatt gttctttggc
     1321 catgtatcat atcctccgtg tctgtttata aactattctt atcataacat agatccgaaa
     1381 tggaaacaag tgttctcgaa caaagtttcg gaaaaagctt gacaaacaaa tgttcgagaa
     1441 ctataataca gacataaaca aaacaaatgt tcgattatag tttgcggagg atgatattat
     1501 gagaagtaag agaagagtga tcggaattat catagcagta gtttttgttt ttctaatgag
     1561 tactttcctt ttaagtaaca acctgatcga ggcccaggga acaaaagaca agcagtttac
     1621 cagcattcag gtgcagaaag gtgatacatt atggaagatc gcaaagaaat atatggggaa
     1681 tgaatattcg tctgttgacg attatattga agaggtatgt cagacaaatc atatatatga
     1741 tggaaagatc acagaagaca tgtatctggt gatcccatat tataaataaa caactggcag
     1801 tcatacggtt gatgtatgac tgccagttgt gaatataaat actttaatac aatattatcg
     1861 cccagttggg tttttgctaa aagatgcttt tgtgctgtta tagacagctt ttccatagtt
     1921 tgtaagatta gagttcattt tctttgcttc agatttgtta gagatgaatc caatctcagt
     1981 taaggtagaa gatgtcttcg tatgacggag aacagctaat cctgtacggt ttacaagacc
     2041 tcggtttgta aatcctgtgg cagaacggat ataaggatga atggttgctg accaattata
     2101 gcttgttagt cctgtagaag atttatatcc tttggaattg tataaggttt cagttcctgt
     2161 agcagaagat ccggcagaat tgatatgaac actgtagaat ctgtctgcgc caacttcatt
     2221 ggcaagatca gaacgctcag taaggcttgg gtatgtatca gataatcttg tatagtaaac
     2281 tttgatatct ggatcgtcat caaagtatga ctttgcagcc tgaacgatct ttaatgtcat
     2341 gtttttttct ttatatccat tggcaactgc tccagagtct gaaccaccat gtcctgcatc
     2401 aagtacaacg atctgtttgt acattgcctt tggtgaatca tagcgaacct tgatataaga
     2461 atctgtctgt gttacttcga atcctttgat cgtagctttt cgaggcctga tatatatctt
     2521 tgtatagcca ttgctgtaag cgacagagta agaatagaag cttgaatcac caataacact
     2581 gcgatgtgac gcatcggaat aataagaaga ataatttcct ttaatgttta cgatcagacg
     2641 atagttattg taatcgtcag ttgttgagat gtcgctctcg gataagccct ctggtttatc
     2701 aagttgtaca taatgttctc catttgttgc tggaagcgtt gtaacggaag aagatgcctt
     2761 attactgtta cttgttgaga aagtaccagc ataagaacta ctgctccatg aataagaata
     2821 tccaagatgt tttgcacaga actcaccagg aacatagatg taattttttt tctgtttata
     2881 ggaatataca cggcgtgcag gtgttggaag tttctttttc tttccattga cataagcata
     2941 tttgctgcca cgtttcatgg tgatcgtctt agaacctttt tttaaagtga tcttccatgt
     3001 tttagaagag taagaataag aaacgcctaa tcccttctgg aatacatagc ttgcggaata
     3061 catggttgta tcaccacgta agaagccagg tgcttttgtt ttgatcgtct taccattata
     3121 gattgcaccg cgagctttct ttttctgctt tttaccagca acttttaaag tgtatgtgcg
     3181 ttttgtcgtt gcataggatg cggcagatac atttgcaggt attgccgtac caatgactga
     3241 aactgataat agtgttgtaa taaataactt ttgtaatctc attgtttatc tccctttctc
     3301 atagattaaa tcgtaacata tttgtaatat taatgcaata aaaagactgt atctctttgg
     3361 atttcctgag atacagtctg atattattag ataaaattaa ttaaaagaat tacagagtga
     3421 ctggtaaaga gcagatccga tcgcctcaga tctagcgtca attcttgcag attccgtgct
     3481 gttagagatg aatcccatct cggcaagggc actcatcatt tttgttcctc gaagtactgc
     3541 aagtccagga cggtatacaa ggcttcggtc ataagaagac ccttttgtat accccattcc
     3601 gcttagcgaa gaggaatgaa tgacactggc atatgttttg taatccttat ataatgtttc
     3661 agttcctttt gcactggcag agtctgcaga attgatatgt acacttaaaa aacggtcagc
     3721 attgacggtg ttagccagat cataacgttc ggtaagagta ggataccaat cagagagtct
     3781 ggtgtaataa accttataca gcggatcaga gtcaaaattt gtcttgatat tctgaacgat
     3841 ctttaaggtc atgttctttt caatataacc gtttccagtt gcgccgctgt cttttccacc
     3901 atgtccggca tcaataacaa tgattctgta gaacatatcc tttggtgccg catacttgac
     3961 atagatataa gaatctgtct gagtaacaga gaatcctttg attgtagatg ttatcagatt
     4021 gatataagtt tttccattta gataagaaac tttataagtt aaagaatctt tgatcgtgcg
     4081 gttagaagct gtgttaaaaa agttgcggta atctccgtca atgatgatct gtaactgctt
     4141 attccagtaa tcatcgttgg aacttataga agacgatgaa agaccatcag gttttttgat
     4201 ccggatagaa taatcagatt cagatgctgt gatttttgtt gtacttccag aaggattgga
     4261 agtctgaggc tttgtatttg atggagtgga aggagcaggt ttagcagtac tgccctttga
     4321 gatcactcct gataacagac gattgttcca tgaataattt aagcctagtt tttttgcaac
     4381 gatatctccg ggaaccatga tataattttt cttttttgcg atataacgga tttttcgagg
     4441 tgcagatgga agggtgaatt tcttcccatt caaggtagct gttctgctgt ttaaagtcat
     4501 cacaagtttc tgagaatttc gttgtaatgt aactcttttt ttggaactcg aatatgaata
     4561 ttttgttcca agagaagaac agtgaccgaa gatccagtat gcagaataca tagaagtaga
     4621 accttcaata tatcctggaa tttttgtact tataacctta ttattatatc tggcattgat
     4681 ggctttcttt ttgacttttt tcttagaatg tatgtactga atcttaaaat atttccctgc
     4741 agctatagaa gctggtgtgg ttattacact tgatagaaga agtgcaagag ataatccagt
     4801 acataaaatt tgttttttgt tcatatataa tacccctcat attcattgtc gattgttagt
     4861 gccattataa tatgttttgt gtagtttttc aacgagaaca tattttctgt gagattaggt
     4921 tagaatgttg tgaaatttct gaaaagttga aaagaattct tttataaaat gaaaaatagt
     4981 ttccaatctg tctaagaatg tggtattcta atatataagc tcgatttttg ggggaaattt
     5041 tctatatttt tacaggaatc gaattatttt gaactatagg agcagataat gaaatcagac
     5101 aatgaatttc gaagaggatt aattgatgga attccaatcg gactagcata tgtggctgtt
     5161 tcgtttgcat ttggaatttc aggagcgtct aagggaattc ctgtatgggc acttactttg
     5221 atctcagcaa cctgtgtaac ttctgcagga cagtttgcag cgatcataac aatgacggca
     5281 ggaggatctt tggtggaatt gattttatca cagatcatta ttaatttaag atattcgatc
     5341 atgtcaattg cgatcggaca gaaattaagt gagaagtcga cagtgtggaa tcggctctca
     5401 atggcattta tggtaacgga tgagattttt gcagtatcct gtgcggggag acatgagatt
     5461 acgcccagat atttttatgc tttgatgtca attccatgga tcagctggac cattggaact
     5521 cttttaggag cgacggccaa tacattatta ccattgatct taaggaatgc acttggactt
     5581 gcaatttatg gaatgttgat cgggattatc atcccaccag ccagacaaga taagcatata
     5641 actatcgtga tcattctttc catgatcgtt agtttattat ttaaatatat caaagcgtta
     5701 tcttttatat ccagtgggtt cgtgatcatc atttgtacct tgatcgcagc aacatttgga
     5761 gcaattttat tcccagtaga agaggaggag actcatgaat cttagcagat tattagttta
     5821 tattgcagtc acagcagttt ctacatatct aatacgagtc atcccattgg ttgcaataca
     5881 gaagaagata gagaatagat tttttcgatc atttttatac tatgttccat atgcagtact
     5941 ggcagcaatg atcatgccag cagtgtttta tgcgacagga aatttgatcg catcgacaat
     6001 tggatttgca attgcgatga tttttgcata taataaaaaa aatattgtta cagttgcatg
     6061 tgcaagctgt atcggagcgt ttatcgcatt agtagtttta gggtaaatta atctgccaat
     6121 tcattgacag acataagtaa tatcaagaag aaatgaggaa gaagatatga attgtaattt
     6181 agcagtcatt aaaggtgacg ggatcgggcc agagatcgtc acagaagcaa tgagagtttt
     6241 agatgctgta ggagaaaagt atggacatac attttcttat acacagatac tgatgggtgg
     6301 agcatccatt gatgttcatg gagaaccatt aacagaggaa gctttagaga ttgctaagaa
     6361 gagtgatgca gtattaatgg gttcgatcgg tggaaataca agtacatctc catggtataa
     6421 acttccagca cagttaagac ccgaggcagg cttattaaaa cttcgtaaat ctcttggatt
     6481 atttgctaac atcagaccag catacttata tgatgaatta aagaaagcat gcccacttcg
     6541 tgatgaagtg
//
LOCUS       DAOMUE010000094         6548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3164_length_6548_cov_2.226398, whole genome shotgun sequence.
ACCESSION   DAOMUE010000094 DAOMUE010000000
VERSION     DAOMUE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3164_length_6548_cov_2.226398"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLC71_05520"
     CDS             <1..635
                     /locus_tag="PLC71_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM02134.1"
                     /translation="PEEITDELIKVIKTEEKVCNYIDMPIQHSENRILQRMGRRTSRE
                     DLVAVIKKLRKEIPDITIRTTLITGFPGESQEDHEGLMNFVEECQFDRLGVFTYSPEE
                     DTLAATFEDQIDEEVKEERRDELMSLQQEISYEHTQQLVGKTIKVMVEGYLFEDDIYV
                     GRSYMDAPKVDGCVFINSPEELMTGDFVYVKITQGREYDVIGEVDYEFTK"
     gene            619..1161
                     /gene="pgsA"
                     /locus_tag="PLC71_05525"
     CDS             619..1161
                     /gene="pgsA"
                     /locus_tag="PLC71_05525"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRM02135.1"
                     /translation="MNLPNKLTILRTLMIPVFLFFLLTDCAGDYSKWIAVVVFILASL
                     TDFLDGHIARKYNLVTNFGKFMDPLADKLLVCSAMIALVGMHRLSSIVAIIIIAREFI
                     ISGFRLIASDNGIVIAASYWGKFKTNFQMFMIIMLIIDLGTSTAVMIENILIVIATAL
                     TIISLVDYLVKNKQVLSEGN"
     gene            1183..2430
                     /locus_tag="PLC71_05530"
     CDS             1183..2430
                     /locus_tag="PLC71_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRM02136.1"
                     /translation="MTAEIICVGTEILLGNIVNTNAAYLSERLASLGISVFFETTVGD
                     NPERLENVIRQGLERSDILILSGGLGPTKDDLTKEIATKACGQELVEDEEALRRLKEY
                     FARSHRNMTENNLKQALVPEDCTVLYNENGTAPGMVIHAKEGKKVVLLPGPPNELLPM
                     FHDQVEPIIKELSSEVLYSDVVKIDCMGESRVAAEIEDLIEKQTNPTVAPYAKLGEVH
                     LRVTAKAVSEDEAKKLVTPITEELYRRFGHKIFTTKEDETLEDVVVDMLSKHHYTIAA
                     AESCTAGMFTARLVNVAGASDVLNESFITYANEAKMKYLGVKEETLNTVGAVSEDTAR
                     QMAEGVARQAGSNVGVGITGLAGPGGETPEKKAGLVYIGVSVNGKTKVNKYQLNGNRQ
                     KVRETAVCRALTMVRHALVEEFL"
     gene            2586..3641
                     /gene="recA"
                     /locus_tag="PLC71_05535"
     CDS             2586..3641
                     /gene="recA"
                     /locus_tag="PLC71_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRM02137.1"
                     /translation="MKQEEKLKALDAALVKIEKDYGKGSVMKLGEYQADRTIEVTPTG
                     SISLDIALGVGGVPKGRIIEIYGPESSGKTTVALHMVAEVQKRGGIAGFIDAEHALDP
                     VYAKNIGVDIDNLYISQPDTGEQGLEITETMVRSGAIDIIIVDSVAALVPKAEIDEDM
                     GDSHVGLQARLMSQALRKLTGVVSKFNCTVIFINQLREKVGVMFGNPETTTGGRALKF
                     YSSIRLDVRRTETLKQAGEMIGNRTRIKVVKNKVAPPFKQAEFDIMFGKGISKYGDIL
                     DLAADADIIHKSGAWYAYNGEKIGQGRENSKKYLEDHPDIAETVEHAIRVHYGLLEED
                     AENANNDPKAETQVSEE"
     gene            3641..4243
                     /locus_tag="PLC71_05540"
     CDS             3641..4243
                     /locus_tag="PLC71_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRM02138.1"
                     /translation="MIITKLDKVGTKQVRLFFDEEKYCLLYYNEIRRLGFHEQDEIGQ
                     EEFEELNKLLLHRAKLKAMSLLKYQDRTKKELKERLMRAEFPEFITEGAVAYVESFGY
                     INDEEYVRRYMEYKSGSKSKIQIKMDLRKKGITAETLERVFEEYEYEEDDILEEQVKK
                     RIRQKGSVTKENFQKYYGYFARKGFNSGKILDLLRKYMED"
     gene            4415..5008
                     /locus_tag="PLC71_05545"
     CDS             4415..5008
                     /locus_tag="PLC71_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HRM02139.1"
                     /translation="MRKKSHISLAGQIMDSMELDNVFDYRLPFYVGSIWPDCRPSFVT
                     TPHKFDITFDDIERKISKFIANYDKDKGMNMRRCAGLGVIIHYIADYFTFPHNDHYPG
                     NVKDHCYYERDLKFGMRAFLQTEEAAQIKEHVAAYDSVEELTSYIRSIHNSYMKLAHT
                     VEEDIRYIVHACTTVVKSVLNMVSYAVGTSVMNIQYV"
     gene            5215..5874
                     /locus_tag="PLC71_05550"
     CDS             5215..5874
                     /locus_tag="PLC71_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM02140.1"
                     /translation="MVNIMVVEDEKPISNLIALSLKKAGYHSECAYDGLEAIDMLEKN
                     YPDLILLDIMLPGADGFEILEYVKPLEIPVIFLTAKGDLNDRVNGLRYGAEDYIVKPF
                     EIMELLARIEVVLRRYHKLDRIINILGLEIDTDAMCVKKEGKEINLTKKEYDTLLLFA
                     RNPGNVLFREIIYERVWGGDYIAGSRTVDLHVQRVRKKVGWEDFLITVPKMGYRLEVP
                     R"
     gene            5871..>6548
                     /locus_tag="PLC71_05555"
     CDS             5871..>6548
                     /locus_tag="PLC71_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HRM02141.1"
                     /translation="MKFRWKLMIITMLIVSFSFGFGGMLLIQYSYQASIKQEKRAARN
                     SYEMILRMLKEINEMDDTYQNQTFASVLKRLNWQGLLRDSSVRLLKEQHGEVQWKQPL
                     YYNRKNDNFRRSNGFSTKQGKAVVFQNKNKVYYQITSKISYGKETMVFQGAYDISEVY
                     ATRHQQLVSFRKIFVLVIGIEIVVSYFLAMILTRPLQKLSLVSKQIADGDYSVRVPVH
                     TEDEIGEL"
BASE COUNT         2342 a          916 c         1515 g         1775 t
ORIGIN      
        1 atccagaaga gatcacagat gagctgatca aagtgatcaa gacagaagag aaagtctgca
       61 attacattga catgccgatt cagcatagtg agaatcgtat cttacagaga atgggaagaa
      121 gaacatcaag agaagatctt gtagcagtga tcaagaaact tcgtaaagag attcctgata
      181 tcacgatccg tacaacactg atcactggat ttccaggaga aagccaagaa gatcatgaag
      241 gattaatgaa ctttgtagaa gaatgtcaat ttgatcgttt aggagtcttt acttattcac
      301 cggaagaaga tacactggca gcaacatttg aggatcagat cgatgaagaa gtcaaagaag
      361 aacgaagaga tgaactgatg agtcttcagc aggagatttc ctatgaacat acccagcagt
      421 tagtcggtaa aacgatcaaa gtcatggtag aaggatattt atttgaagat gatatctacg
      481 ttggacgttc ttatatggat gcaccaaagg tagatggatg tgtttttata aattctccag
      541 aagaattgat gacaggagac tttgtttatg ttaaaattac tcagggtaga gaatatgatg
      601 tgataggaga agtagattat gaatttacca aataagttaa caattttaag aacacttatg
      661 atcccggtat tcttattttt cttgctgaca gattgtgcag gggattatag caagtggatc
      721 gcagttgttg tatttattct agcgagtctt acagactttt tagatggaca tattgcaaga
      781 aaatacaatc tggtaacaaa cttcggaaaa tttatggatc cattggcaga caaattatta
      841 gtttgttctg caatgatcgc attagttgga atgcaccgtt tatcatctat cgtagcgatc
      901 attattattg caagagaatt tatcattagt ggattccgcc tgatcgcctc tgataatgga
      961 atcgttatcg cagccagcta ctggggaaaa tttaagacaa actttcagat gtttatgatc
     1021 attatgctga tcattgatct tggaacaagc acagctgtga tgatcgagaa tattttgatc
     1081 gtcattgcaa cagccctgac aattatttct ttagttgatt atttggtgaa gaataaacag
     1141 gtattatctg aaggaaatta gatccagaaa ggaacaacaa ttatgacagc agaaattatt
     1201 tgcgtaggaa cagagatttt attaggaaac atcgtgaata ccaatgctgc ctacttatca
     1261 gaacgcttag cctcattggg gataagcgtt ttttttgaga cgacagtagg cgacaatcca
     1321 gaaagattag aaaatgtgat cagacaggga cttgaaagat ctgatatttt gatcttatct
     1381 ggtggacttg gaccaacgaa agacgatctt acaaaggaaa ttgcaacgaa agcatgtgga
     1441 caggagcttg tagaagatga agaagcacta agacgcttga aagaatattt tgcgagaagt
     1501 catcgtaaca tgacagagaa taatttaaaa caggcattag tgccagaaga ctgtactgtt
     1561 ttatataacg aaaatggaac agcaccggga atggtcattc atgcgaaaga agggaagaaa
     1621 gtagttttac tcccaggacc tccaaatgaa ctgcttccaa tgttccatga tcaggtagaa
     1681 ccgatcataa aagaattaag ttcagaggta ttatactccg atgttgtgaa gattgattgt
     1741 atgggagaaa gccgcgttgc agcagagatt gaagatttga tcgaaaagca gacgaatcct
     1801 acagttgccc catatgccaa attaggagaa gttcatcttc gagtgacagc aaaagcggta
     1861 agtgaagatg aagcaaagaa gcttgttaca ccaataacag aagaattata cagaagattt
     1921 ggtcataaga tttttacgac aaaagaagat gaaacattag aagatgttgt ggttgatatg
     1981 ttaagtaaac atcattatac gatcgcagca gcagaatcct gtacagcagg aatgtttaca
     2041 gcaaggttag ttaatgttgc aggagcatcg gatgtcttaa atgaaagttt cattacatat
     2101 gcaaatgaag ctaagatgaa atatcttgga gtaaaagaag aaactctaaa taccgttgga
     2161 gcagtttcag aagatacggc cagacagatg gcggaaggtg ttgcacgaca ggctggttca
     2221 aatgtcggtg ttggaatcac aggtcttgct ggaccgggcg gtgaaacacc tgagaagaag
     2281 gcaggacttg tttacatcgg tgtcagtgtt aatggaaaga cgaaagtaaa caaataccag
     2341 ttaaacggta acagacagaa agtcagagaa acagctgtat gcagagcgtt aactatggta
     2401 agacatgcat tggttgaaga gtttctatga gaatcatcat aagaaaaagg atcacgaata
     2461 aaaaccgaac aaatattcgc tcgaacactt gttttttagt gtaaggatat gctatactct
     2521 aaacaggaaa ttaaaagata gaagaatata agataaatat ttgaaattaa aaggagtaaa
     2581 catatatgaa acaggaagaa aaattaaagg cattggatgc cgcattggtt aagatcgaga
     2641 aagactatgg taagggatct gtcatgaaac ttggagagta ccaggcagac cgtacgattg
     2701 aagtaacacc aacaggttct atcagtcttg acatcgcatt aggagtaggt ggagtaccta
     2761 agggacgtat catcgagatt tatggacctg aatctagtgg taagacaaca gtagcacttc
     2821 atatggtagc tgaagtccag aagagaggcg ggattgcagg atttattgat gcagagcatg
     2881 cccttgatcc ggtctatgca aagaatatcg gggttgacat tgataacctt tatatctctc
     2941 agccagatac aggagagcag ggacttgaaa tcacagagac aatggtaaga tctggagcga
     3001 tcgacatcat tatcgtcgat tctgtagctg cattagtacc gaaggcagag attgatgaag
     3061 atatgggaga ttcgcacgta ggtcttcaag caagattgat gtcacaggca cttcgtaagt
     3121 taacaggtgt tgtaagtaag tttaactgca cagttatctt tatcaatcag ttgcgtgaga
     3181 aagttggagt catgtttgga aacccagaga caacaactgg tggacgtgca cttaagttct
     3241 attcatctat ccgtttagat gtcagaagaa cagagacatt aaaacaggca ggagagatga
     3301 tcggtaacag aacaaggatt aaagtagtga agaataaggt ggctcctccg tttaaacaag
     3361 cagaatttga tattatgttt ggaaaaggaa tttccaagta tggagatatt ttagatcttg
     3421 cagcagatgc agatatcatt cataagagtg gtgcatggta tgcatacaac ggagagaaga
     3481 ttggacaggg tagagagaac agcaagaagt acttagaaga tcacccggat atcgcagaga
     3541 cagtggaaca tgcaatccgt gttcactatg gactgttaga ggaagatgct gagaatgcca
     3601 acaacgatcc taaggcagag actcaggttt ctgaagaata atgatcatta cgaaactaga
     3661 taaagtagga acaaagcagg tcaggctttt cttcgatgaa gaaaagtact gcctgcttta
     3721 ctataatgag ataagacgat tgggattcca tgaacaagac gagatcggac aggaagaatt
     3781 tgaagaatta aacaaattac tgttgcatcg ggcgaagtta aaagctatgt cattgttaaa
     3841 atatcaggat cgtacaaaaa aggaattaaa agaacgtctt atgagagcag aatttccaga
     3901 atttattacg gaaggggcag ttgcgtatgt ggagtcattt ggatatatca atgatgaaga
     3961 atatgtacgc cgctacatgg aatacaagtc aggaagtaag agtaagattc agattaagat
     4021 ggaccttcgg aagaaaggaa tcactgcgga gacattagag agggtctttg aggaatatga
     4081 atacgaagaa gatgatatct tggaagaaca ggttaagaag aggatccggc aaaagggttc
     4141 cgtcactaag gagaattttc agaaatatta cggatatttt gcgagaaaag gatttaatag
     4201 cggcaaaata ttagatttat tacggaaata catggaagat taaataaatt acaggctaaa
     4261 tattaaaaaa atataaagaa ttcatcattt ggttgcatat tgacagataa aattatataa
     4321 tttgagtcaa agggtgtaac aaagcagatg acgacgatat ctggaaggaa tatatgatcc
     4381 ggatatggtt ccaattataa ggagtatcat ttatatgaga aagaaatcac atatttcatt
     4441 agcagggcag attatggatt ctatggaact tgacaatgta ttcgattata gattaccgtt
     4501 ttatgtagga agtatctggc cagactgcag accatcattt gtgacgacac ctcataagtt
     4561 tgatattaca tttgatgata ttgaacgtaa gatttcaaaa tttattgcca attacgataa
     4621 agataaggga atgaatatgc gacgttgtgc aggtcttggt gtgatcattc attacattgc
     4681 agattatttt acattcccac ataatgatca ttatccaggc aatgtaaagg atcattgcta
     4741 ttatgaacgt gatctgaagt ttggaatgag agcatttctt cagacagaag aagcagcaca
     4801 gatcaaggag catgttgcgg catatgattc tgtggaagaa ttgacatcat atattagaag
     4861 tattcataat tcttatatga agcttgcaca tacagtagaa gaagatatca gatatatcgt
     4921 acatgcgtgt acaaccgttg tgaagagtgt actgaatatg gtaagttatg cagttggtac
     4981 aagtgtgatg aatattcagt atgtatagga caaggaattg aagataaaat aataaagaaa
     5041 aagggcaaag acagacagag atgatttgtt tttgctcttt ttattgaaat cagtttgtaa
     5101 aaaatgataa aaatatgatg caagtatgat gcaaagaatt cgtaaaatag aaaaggtttt
     5161 gggcagcata caaaaacaat aaggaaaatc aataaaacaa taaggagaac aattatggtg
     5221 aacatcatgg tcgtagaaga tgaaaagcca atttcaaatc tgatcgcact aagtttaaag
     5281 aaagccggct atcatagtga atgtgcttat gatgggttag aagcaattga tatgctggaa
     5341 aagaattatc cagatttgat cttactagat atcatgctgc ccggggctga tgggtttgaa
     5401 attttagaat acgtaaaacc attagagata ccagtcattt tcctgacagc aaaaggagat
     5461 cttaatgaca gagtaaatgg tctgcgatat ggagcagaag actatattgt aaaaccattt
     5521 gagattatgg aattattggc aaggattgag gttgtattaa gacgatatca caaattagat
     5581 cgtatcatca atatattagg tttagaaatc gacacagatg caatgtgcgt aaaaaaagaa
     5641 gggaaagaaa ttaacttgac caagaaggaa tatgatacat tattgctgtt tgcaaggaat
     5701 cctggaaatg tattattcag ggaaataatt tatgaacgtg tttggggtgg agattacatt
     5761 gcgggaagca ggacggtaga tcttcatgtg cagagagttc gaaagaaagt tggctgggaa
     5821 gattttttga tcactgtgcc caagatggga taccgtctgg aggtaccaag atgaaatttc
     5881 gttggaaatt aatgatcata acaatgttga tcgtttcatt ttcatttgga tttggaggaa
     5941 tgttgctgat tcaatattca tatcaagcat ctatcaaaca agaaaaaaga gcagcaagga
     6001 attcttacga aatgatctta agaatgttaa aagaaattaa tgagatggat gatacgtatc
     6061 agaatcaaac atttgcgagt gtattgaaac gattaaactg gcagggcttg ctaagagaca
     6121 gctcagttcg attattaaag gaacaacatg gagaggttca atggaagcag ccactttatt
     6181 acaatcgtaa aaatgataat tttaggagaa gcaatggttt ttcaacgaaa cagggaaaag
     6241 cggtagtatt ccaaaataaa aataaagtat attatcagat tactagtaag atttcttatg
     6301 gaaaggaaac tatggttttt cagggagcat atgatatttc agaagtatat gcgacaaggc
     6361 atcagcagtt ggtaagtttt cgtaagattt ttgtattagt aataggaatt gagattgtag
     6421 tatcctattt tctggcaatg atccttacaa gaccattaca gaaattgtcg ctggtttcga
     6481 agcagattgc agatggtgat tattctgtca gggttccagt gcatacagaa gatgaaattg
     6541 gagaactt
//
LOCUS       DAOMUE010000095         6543 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3168_length_6543_cov_2.242756, whole genome shotgun sequence.
ACCESSION   DAOMUE010000095 DAOMUE010000000
VERSION     DAOMUE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3168_length_6543_cov_2.242756"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /gene="aspS"
                     /locus_tag="PLC71_05560"
     CDS             complement(<1..1007)
                     /gene="aspS"
                     /locus_tag="PLC71_05560"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRM02142.1"
                     /translation="MAESMSGLARSHRCTEVSNKLIGTEVTVMGWVQKRRNLGSLIFV
                     DLRDRSGILQLVFDQEVLSEEDYNKANSLRSEYVVAAVGTVEKRSAAINENLKTGDIE
                     IKVHTLRILSESETPPFPIDENQTVKDELRLKYRYLDLRRPNLQRNLRIRNDVTRMVR
                     NFLGDEGFLEIETPVLGKSTPEGARDYLVPSRVHPGEFYGLPQSPQLFKQLLMCSGFD
                     RYYQIAKCFRDEDLRADRQPEFTQIDMELSFVDVDDVIDVNERLLKKIFKEVLDIDVQ
                     LPMLRMTYKEAMDRFGSDKPDMRFGMELTNVSDTVAGCDFMVFKGALENGGSVRGLNA
                     KG"
     gene            complement(1084..1602)
                     /locus_tag="PLC71_05565"
     CDS             complement(1084..1602)
                     /locus_tag="PLC71_05565"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRM02143.1"
                     /translation="MSEYAIVAPEDFDQSVFRLIGKEWMLVTAKNQEGKVNTMTASWG
                     GLGVMWGKNVAVTVLRPQRYTKEFIDQSESFTLSFYDDTFKKDLSYLGSVSGRDEDKI
                     AKTRLTPTDANGIPFFEEAKIVLICKKLYSQPMDPAGFVDKSLDEQWYPEKDYHVLYA
                     AEIEQILVRKDS"
     gene            complement(1688..2932)
                     /gene="hisS"
                     /locus_tag="PLC71_05570"
     CDS             complement(1688..2932)
                     /gene="hisS"
                     /locus_tag="PLC71_05570"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRM02144.1"
                     /translation="MITQAPRGTKDWFGKDMDQRTYLENIFKDLCASYNIHEIITPVF
                     EHTELFQRGVGETTDVVQKEMYTFEDKGHRSISLKPEGTAGAIRAYLQNGLANEPQPI
                     KMFYITPAFRYEKPQSGRLRQHHQFGVEFVGAESPLAEVELITLVSTFIKKIGLKDAK
                     LHINSIGCENCRKEYNKALLAYLKEHEEHLCPTCRERMEKNPLRVIDCKVPECKEIVK
                     DAPRTIDYLDDECKAHFEELKSLLDALNIPYEVDTEIVRGLDYYTKTVFEFVNEDGFT
                     LCGGGRYDNLVHEIDGKVSMPSVGFGMGVERILYFLEEEKVDLPCTRDIDLYVGILGQ
                     EAKAKAYQIVVDLRNQGFVVETDYLGRSVKAQMKYANKLNAKNTIIIGDDELAKNEGN
                     VKNMETREVTTISLDKIADCLK"
     gene            complement(2929..3552)
                     /locus_tag="PLC71_05575"
     CDS             complement(2929..3552)
                     /locus_tag="PLC71_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HRM02145.1"
                     /translation="MAISYGRTDIETPIHADIKETISHGIIVSNLAYLVGKELGLQEE
                     VCYDLAVAGMLHDLGKVRLNFYMNEKVEDEILAVDETRYMRMHPSIGYAILADYDYNQ
                     TVLDAVLYHHEHYDGTGYPHNLVGKQIPLVGRIMHVCDIFGALISNRDYREGFDVETA
                     LDIMIDEVKNFDMKVFLAFQRVAFSDGVMETVMEKGNLDELFEEEQE"
     gene            complement(3567..>4102)
                     /locus_tag="PLC71_05580"
     CDS             complement(3567..>4102)
                     /locus_tag="PLC71_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HRM02146.1"
                     /translation="QDIKEKFNLARQAGIDNINMDMICGLPEEGMEELSYTLDEIKKL
                     DPESLTVHALAVKRSSRLNRMKDTYHFGASEEMVSYAASCARDMNMEPYYLYRQKNIP
                     GNLENVGFSKKGKECLYNILIMEELHDIIAVGAGTSSKIVHQEDHQVDRIENLKDIKQ
                     YITRIDEIIHRKELKMI"
     assembly_gap    4103..4114
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4115..4968)
                     /locus_tag="PLC71_05585"
     CDS             complement(<4115..4968)
                     /locus_tag="PLC71_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM02147.1"
                     /translation="MIGLIQNKTIYEYDIRSLILAFMLGEKIELTDHVDSIYDFILDV
                     DYKDQEIVMNLYKKGELEDEIQLFGDYENKKIFKNRMKQGIYQLFSKALDKQLPWGTL
                     TGIRPTKIAFDGYEKGESSEEIIHRFQKDYLASEEKARLCTETVQKEKELLKAFPYKE
                     GYSLYIGIPFCPSTCLYCSFTSYSIDQFENIVQDYLEALLKELDFVAKTYAHKELQTM
                     YIGGGTPTSLKEEDLEYLLYEVNKRFPFSKIQEITVEAGRPDSLNFEKLKILKKYGVT
                     RISINPQS"
     gene            complement(4972..5631)
                     /locus_tag="PLC71_05590"
     CDS             complement(4972..5631)
                     /locus_tag="PLC71_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM02148.1"
                     /translation="MSETTLRLDRPLKMVTICLGQMQTNCYIVENTKTNQAFVFDPGD
                     QGERIMNTLEEDGMELAGVCLTHGHFDHVMALPYLMEQKPDLDIYLYEDEADVLADPQ
                     LNLGAMMGMDLSLKANHLVKDGEIIDVLGTKVKCIHVPGHTKGGVCYYFADYGWLISG
                     DTLFQMSIGRTDFPTGNLNDLLTAIREKLFVLPPWTKVLSGHTPPTTIEEESRCNPFL
                     Q"
     gene            complement(5640..>6543)
                     /locus_tag="PLC71_05595"
     CDS             complement(5640..>6543)
                     /locus_tag="PLC71_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5913 domain-containing protein"
                     /protein_id="HRM02149.1"
                     /translation="LQNGDRVEIITSQNSKGPSRDWLGIVKSAQARSKINQWFKRQFK
                     EENIVRGKELLEKYCKSKSIRLPELMKPEYIKKVELKYGFKDWDSVCAAVGHGGLKEG
                     QVINKMQEEDRKIQKQKITDEQILQKTTEAAQAAAANPEKKKDEKSKGGIVVRGANDV
                     AVRFSKCCNPVPGDEIVGFITRGRGISIHRTDCVNLLNMPESDRARLIEAEWQVSEAD
                     TTQTYTTEINIYANNRKGLLAEVSKIFLELDIDIITINVSNNKKGRATLSMSFDITGV
                     EQLNRIIAKIRNVEGVIDIERTAG"
BASE COUNT         1716 a         1488 c         1008 g         2319 t
ORIGIN      
        1 cctttagcgt ttaatcctct tacggatcca ccattctcta atgctccttt aaataccata
       61 aagtcgcatc cggcaactgt atcggataca ttcgtaagtt ccattccaaa acgcatatct
      121 ggtttatcag atccgaaacg gtccattgct tctttataag tcatgcgaag cataggaagc
      181 tgtacatcaa tatctaatac ttctttgaag atcttcttta acagacgttc atttacatcg
      241 atcacatcat cgacatcaac aaatgaaagt tccatatcga tctgtgtaaa ctctggctgg
      301 cggtctgcac gtagatcttc gtcacggaaa cattttgcga tctgatagta acggtcgaat
      361 ccagagcaca ttaaaagctg tttaaacagc tgtggtgact gtggaagtcc gtaaaattct
      421 cctggatgta cacggcttgg tacaagataa tctcttgctc cttctggtgt actctttcct
      481 aaaactggtg tctcgatctc taagaatcct tcatccccta agaagttacg taccattctt
      541 gttacatcat tacgaatgcg taagtttctc tgaagatttg gtcttcgaag atcaagataa
      601 cggtatttta aacgaagttc atctttgact gtctgattct cgtcaattgg aaatggagga
      661 gtttctgatt ctgataagat acgtaatgta tgtaccttaa tctcgatatc tcctgtcttc
      721 aagttctcgt tgatcgctgc acttctcttc tctacagtac ctactgctgc aacgacatac
      781 tcacttctta aagagtttgc cttattatag tcttcctcag ataatacttc ctgatcaaaa
      841 actaactgta agattcctga gcggtctctt aagtctacaa atataagact tcctaaattt
      901 cttcgtttct gtacccatcc catgacagtg acttctgttc cgataagttt gttgcttacc
      961 tctgtacatc ggtgggatct ggctaaacct gacattgatt ctgccatgat atttcctcct
     1021 tcatcctttt cactttgtac gaaaggcaga gcccgtatct tcggttctgc cttatatatt
     1081 cttttatgaa tcttttctta caaggatctg ttcgatctct gcagcgtaaa gtacgtgata
     1141 atccttctct ggataccact gttcatctaa agatttatca acaaatcctg ctggatccat
     1201 tggctgagaa tataacttct tacagatcag tacgatcttt gcttcctcaa aaaatggaat
     1261 tccattcgca tctgttggtg tcagacgtgt cttcgcgatc ttgtcttcat ctcttccaga
     1321 tacacttcca agatagctta aatccttctt aaaagtatca tcatagaaag ataatgtaaa
     1381 tgattctgac tggtcaatga attcttttgt atatctttgt ggtcttaaga ctgtcacagc
     1441 aacattctta ccccacataa cacctaaacc accccatgag gcagtcattg tgttaacttt
     1501 tccttcctga ttctttgcag taacaagcat ccattcctta ccaatcaggc gaaatacgct
     1561 ttggtcgaaa tcttctggtg ccacgatcgc atattcactc atgcatgtta cctcctttct
     1621 tgtaattatt actcattata tatgattttg tcaaaaatgt cacgaactag tataagaaag
     1681 catgatttta ttttaaacaa tctgcgatct tatcaagcga aatggtcgta acttctcttg
     1741 tttccatatt tttgacattt ccttcattct tcgctaattc gtcatcacca atgatgatcg
     1801 tattcttggc attcagctta tttgcatatt tcatctgtgc ttttacgctt cttccaagat
     1861 aatctgtttc tacgacaaat ccctgattac gaagatctac aacgatctgg taagcttttg
     1921 cttttgcttc ctgtccaaga attcctacat aaaggtcaat atctcttgtg catggaagat
     1981 ctactttctc ttcttctaag aaataaagga ttctctctac acccatacca aatccaacgg
     2041 atggcataga aacttttcca tcgatctcat gaacaagatt gtcgtaacgt ccaccaccac
     2101 ataaagtaaa tccatcttca tttacaaatt cgaatacggt ctttgtatag tagtctaatc
     2161 cacgaacgat ttctgtatca acttcatatg gaatatttaa tgcatctaac agactcttta
     2221 attcttcaaa atgtgcttta cattcatcat ctaaatagtc gatcgttctt ggtgcatctt
     2281 tgacgatctc tttgcattct ggaactttac agtcaatgac acgtaatgga ttcttttcca
     2341 tacgttctct gcatgtagga cataaatgtt cttcatgttc ttttaaatat gctaataatg
     2401 ctttgttgta ttcttttcta caattttcac atccaatgct gttgatgtgt aattttgcat
     2461 cttttaaccc aattttctta ataaaagtgg atactaatgt gatcagctct acttctgcta
     2521 atggactttc cgctccgaca aattcaactc cgaactggtg gtgctgtctt aatcttccac
     2581 tctgtggttt ttcatagcgg aatgctggtg tgatatagaa catcttgatc ggctgtggct
     2641 catttgccag accattctgt aaatatgcac ggatcgctcc tgctgttcct tctggtttta
     2701 atgaaatgct tctgtgtcct ttatcttcaa atgtatacat ctctttctgg acaacgtctg
     2761 ttgtttctcc aacacctctc tgaaaaagct ctgtatgctc aaatactggt gtgatgatct
     2821 cgtgaatatt ataggatgcg cataaatctt tgaaaatatt ctctaaatat gtgcgctggt
     2881 ccatatcttt tccaaaccag tcttttgttc ctcttggtgc ctgtgtaatc attcttgttc
     2941 ctcctcaaat aattcatcca gattgccttt ttccatcaca gtttccataa ctccgtctga
     3001 aaatgcgact ctctggaatg ctaaaaatac tttcatatca aaattcttta cttcatcgat
     3061 catgatatca agtgcagtct caacatcaaa tccctctcta taatccctgt ttgagatcaa
     3121 tgctccgaaa atatcacata catgcatgat ccttccaacc aatggaatct gttttcctac
     3181 cagattatgt ggataaccgg taccatcata atgttcatga tgatatagaa ctgcgtctaa
     3241 aaccgtctgg ttataatcgt aatctgcaag gatcgcatat ccaatggatg gatgcattct
     3301 catatatctt gtttcgtcaa cagccagaat ctcatcttcc actttctcat tcatatagaa
     3361 attaagtctt acttttccaa gatcatgtaa cattcctgca accgcaagat cataacacac
     3421 ttcttcttgt aatcctaatt ccttgccaac aagataagcc aaattactga caatgatacc
     3481 atgactgatc gtttctttta tatccgcatg gatcggtgtc tcaatatctg ttcgtccata
     3541 actgattgcc ataatcaaac tcctgtttat atcatcttta attcttttct atgaatgatc
     3601 tcatcaattc gtgtaatgta ttgctttata tctttaagat tttcgatgcg gtccacctga
     3661 tggtcttcct gatggacgat ctttgaagat gtaccagcac caactgctat aatatcatgt
     3721 aattcctcca taatcaaaat attatagaga cattcttttc ctttttttga aaaaccgaca
     3781 ttctcaaggt tccctggaat gtttttctgc cggtataaat aatatggttc catattcata
     3841 tctcttgcac atgaagcggc ataagatacc atctcttcac tcgctccaaa gtgatatgta
     3901 tctttcattc gattcaatct cgatgaacgc ttgactgcaa gtgcatggac cgttaaactc
     3961 tctggatcaa gttttttgat ctcatctaaa gtataggaaa gttcttccat gccttcttct
     4021 ggaagcccac agatcatatc catattaatg ttatcgatcc cagcctgtct cgcaagatta
     4081 aatttctctt tgatatcttg tgnnnnnnnn nnnnattgac tgtggattga tactgatcct
     4141 tgtcacacca tatttcttta gaatctttaa tttctcgaaa ttcaagctgt ctggccgtcc
     4201 tgcttccaca gtgatctcct ggatctttga aaatgggaat ctcttattaa cctcatataa
     4261 cagatattcc agatcttctt cttttaaaga tgttggtgtt ccaccgccaa tatacatcgt
     4321 ctgaagttct ttgtgcgcat aagtcttcgc cacaaaatcc agttctttca ataaagcttc
     4381 aagatagtct tgtacaatat tctcaaattg atcgatcgaa tacgatgtaa aagaacagta
     4441 caaacatgtg cttgggcaaa atgggattcc tatatataga ctgtatcctt ctttgtatgg
     4501 aaatgctttt agcagttctt tttctttctg aacagtttct gtacataatc ttgctttttc
     4561 ttcggatgcc agataatcct tttgaaatct gtgaattatc tcttcactgc tctcaccctt
     4621 ttcatatcca tcaaaagcaa tctttgtcgg acggatccca gtcaacgttc cccatggaag
     4681 ctgtttgtct aatgctttgg aaaacaactg atagattcct tgtttcatcc ggtttttaaa
     4741 aatcttctta ttttcataat caccaaataa ctggatctca tcttctaact cgcccttttt
     4801 atatagattc atgacgattt cctgatcttt ataatccacg tccagtataa aatcatagat
     4861 agaatcaacg tgatccgtca attctatctt ctctccgagc ataaatgcca atattaaact
     4921 tcgaatgtca tattcataaa tggtcttgtt ctgaattaat ccaatcataa tctattgcag
     4981 aaacggattg catctgcttt cctcctcgat cgtcgttggt ggtgtatgac cggatagtac
     5041 ctttgtccat ggtgggagta cgaataattt ctctctgatc gctgtcagaa gatcattcaa
     5101 attgcctgtc ggaaagtctg ttcttccaat gctcatctgg aacagtgtat ctccagagat
     5161 cagccatcca taatctgcaa aataataaca tactccacct tttgtatgtc ctggaacatg
     5221 gatacatttt acctttgttc ctaatacatc tatgatctct ccatctttca ccagatgatt
     5281 cgcttttaat gagagatcca ttcccatcat cgctccgaga ttgagctgtg gatctgccag
     5341 aacatctgct tcatcttcat aaagatagat atcaagatct ggtttttgtt ccatcagata
     5401 tggaagggcc attacatgat caaaatgccc atgtgtcaga catacgcctg ccagttccat
     5461 tccatcttcc tctaatgtat tcataatacg ttctccctga tctcccggat caaagacgaa
     5521 tgcctgattt gtttttgtat tctcaacaat atagcagttt gtctgcatct gcccaagaca
     5581 gatcgttacc atcttaagag gtctgtcaag tcttaatgtt gtttcgctca tattttcacc
     5641 tatcctgctg ttctttctat gtcgatcacg ccttctacat tcctgatctt cgcaataata
     5701 cggttcaact gctctacgcc tgtaatatca aaggacatac taagcgttgc tcttcctttc
     5761 ttattgtttg atacgttgat cgttatgatg tcaatatcta attctaagaa gatctttgat
     5821 acttctgcta acaatccctt tcgattattg gcataaatat tgatctctgt cgtgtaagtc
     5881 tgtgttgtat ctgcttcact tacctgccat tcagcttcaa taagtcttgc cctgtcagat
     5941 tctggcatat tcagaaggtt cacacaatct gttctgtgaa tcgagattcc tcgtcctctt
     6001 gtaataaatc caacgatctc atctcctggt actggattac aacattttga aaaacgaaca
     6061 gcaacatcat tcgctccacg tacaacaatt cctcctttgg atttctcatc ctttttcttc
     6121 tctggattcg ctgctgccgc ctgtgctgct tctgttgttt tctgcaagat ctgttcatcc
     6181 gtgatctttt gtttctggat cttacggtct tcttcctgca tcttattgat aacctggcct
     6241 tcttttaaac caccatgtcc aacagctgca catacagaat cccagtcttt aaatccgtat
     6301 tttagttcaa ccttcttaat gtattcaggt ttcatgagtt ctgggagcct gatgctcttg
     6361 ctcttacagt atttttctaa cagttcctta cctctgacga tattctcttc cttgaattgt
     6421 cgtttaaacc actgattgat cttacttctt gcctgtgctg attttacgat tcctaaccag
     6481 tctctgctag gtccttttga attctgtgat gtaatgatct ctacacgatc accattctgc
     6541 aaa
//
LOCUS       DAOMUE010000096         6524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3183_length_6524_cov_2.812336, whole genome shotgun sequence.
ACCESSION   DAOMUE010000096 DAOMUE010000000
VERSION     DAOMUE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3183_length_6524_cov_2.812336"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="PLC71_05600"
     CDS             <1..125
                     /locus_tag="PLC71_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRM02150.1"
                     /translation="GIGACLACVCKSKDVDSHSHVHNKRVCKDGPVFDAREVEL"
     gene            125..1027
                     /locus_tag="PLC71_05605"
     CDS             125..1027
                     /locus_tag="PLC71_05605"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRM02151.1"
                     /translation="MNLSVDIAGVTMKNPVTTGSGTFGSGEEFSEFVDLSKLGAVTTK
                     GVANVPWPGNSVPRVAEVYGGMLNAIGLQNPGIDVFVERDIPYLKKAGATIIVNVCGK
                     SEKDYLEVVERLADQPVDLLEINVSCPNVKEGGIAFGQDPKALYDITKAVKAKAKQPI
                     IMKLSPNVTDITEMAKAAVDAGSDALSLINTLTGMKIDIERRDFVLANKTGGMSGPAI
                     KPVAVRMVYQVANAVDVPIIGMGGISTAEDALEFIMAGATAVSVGTANFINPATTLEV
                     VDGIKTYMERHQINDINELIGCVK"
     gene            1050..1469
                     /locus_tag="PLC71_05610"
     CDS             1050..1469
                     /locus_tag="PLC71_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRM02152.1"
                     /translation="MNILFFLTPKSEVAYIPADESLRQAFEKLKYHGYSAVPTLTDDG
                     KYAGTITEGDVLRAMESMCHWNISEAEKVKLMDVKRKRNIQAVTISTDMDDLVDVITD
                     QNFVPVIDDQEKFIGIVTRKDVIRFYHDEYRKLEKNE"
     gene            1490..2173
                     /gene="pyrE"
                     /locus_tag="PLC71_05615"
     CDS             1490..2173
                     /gene="pyrE"
                     /locus_tag="PLC71_05615"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRM02153.1"
                     /translation="MEQYKREFIEFMVESDVLKFGEFTLKSGRKSPFFMNAGAYVTGS
                     QLKRLGEYYAKAIHSTYGDDFDVLFGPAYKGIPLGVVTAIAYSELYGKEVRYCSDRKE
                     AKDHGADKGNFLGSELKDGDRVIMIEDVTTSGKSMEETVPKVKGAANVEIVGLMVSLN
                     RMEIGKGGEKCALDEVKELYGFDTAAIVDMEEVVECLYNKECNGKVVIDDTLKAAIDA
                     YYEQYGAKH"
     gene            2176..2661
                     /locus_tag="PLC71_05620"
     CDS             2176..2661
                     /locus_tag="PLC71_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM02154.1"
                     /translation="MVLVTVEKKKLARRGIKVIFYLYIIGIFYFVLLSERYGRDTGYD
                     TSHVNLVLFKEINRFWTYRHLLSTEAVVTNLFGNIFAFSPFGFMIPIVINKKKAFFRA
                     VFATSFFSLVIETSQLIMKVGVFDVDDLLMNTVGGLIGYMIYRVVVAVYDLYNRKRAA
                     K"
     gene            2658..2996
                     /locus_tag="PLC71_05625"
     CDS             2658..2996
                     /locus_tag="PLC71_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6142 family protein"
                     /protein_id="HRM02155.1"
                     /translation="MKRNKRKTFAYRPSDKIISRESIVSVILGAAGIIGYYLLLFGSI
                     KTGGTSSPLSGAGGWILLVVSAIGLYFGIQSMKDTAAVMKWKIIGCTCNGIVLAVSVI
                     IFVIGIIAMI"
     gene            3027..5030
                     /locus_tag="PLC71_05630"
     CDS             3027..5030
                     /locus_tag="PLC71_05630"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRM02156.1"
                     /translation="MLSERHELNKIALLELKKDVEETREFSSYFSKMADLLLYVNEIY
                     DTKERTLEQLKQWNRIWYEEISQEQYAHSFGNPEFCTEKFGKEYGQIFAFLYAEMRSG
                     FIYAIEGRLFDLVINNELFLEISNLFLSGEEHPQKIKHIVFDHMRDYSEDVFEYRIRE
                     QLDPELDFATKIVMESDLSDPDILYQYGEYISENEIDTLKFLNKLPQEEITAMAKTYV
                     DGFHEGFIINNIDMSPKRSVNIRYTIGFEPVVREAVRLFSEIGLAPIIYRSGVSVLTR
                     GLHKIGYVSSSPNPQYEYDHRYDQAIFFDNRFMKHKLECYHNAYEHYKDEAYVFAGPA
                     CMETFGEIPFLPENKEENLKLSKKQQELSVEFQMKASEIMNQYIKPEQRSFTIISYPI
                     PEIGDNFEEIFKEVVKVNTLDKDKYRQIQQHLIDALNQAVEVHIKGKGKNKTDLYVAM
                     HEMDDPSKETNFENCLADVNIPVGEVFTSPKLTGTNGVLHVGEVYLNDLKFIDLEITF
                     KDGMISEYTCKNFKTEEENKAFIKENLLYNRDTLPMGEFAIGTNTTAYVMAKTYDILY
                     QLPILIVEKMGPHFAVGDTCYSHCEETEVHNPDGKEIVAKYNECSKEGEYFQCHTDIT
                     IPYEELDSICAITLGGDEILLIKDGKFVLSGTETLNEPLTEIG"
     gene            5106..5609
                     /gene="purE"
                     /locus_tag="PLC71_05635"
     CDS             5106..5609
                     /gene="purE"
                     /locus_tag="PLC71_05635"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRM02157.1"
                     /translation="MAKVAIVMGSDSDMPIMKKAAEFLDQMGIDFEMTIISAHREPDI
                     FFEWAKGAKDRGVKVIIAGAGKAAHLPGMCAALFPMPVIGIPMKTSDLGGVDSLYSIV
                     QMPSGIPVATVAINGGLNAGILAAKILATSDEKLLEKLEAYSEEMKQSVVEKAEKLDK
                     VGYKNYE"
     gene            5602..6312
                     /locus_tag="PLC71_05640"
     CDS             5602..6312
                     /locus_tag="PLC71_05640"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM02158.1"
                     /translation="MNKKILAAFLCVCMVVVTGSFPVSAAKTKKTYKIVIDAGHQGKG
                     NSKTEPIGPGAKMRKPKVAYGTQGVKTKVPESKLTLQIALKLEKELKKRGYDVYMIRR
                     KQNVNISNKQRAIRANKSGADICIRLHADAASANVRGVSVLYPSQQNPYVAKLSKKSK
                     KLSKNILDSYCKSTKFKKRGLSKRDDLTGTNWSKIPVALIEMGFMTNKTEDKQMQKKQ
                     TQKKMVDGIANGIDQYFK"
     gene            6331..>6524
                     /locus_tag="PLC71_05645"
     CDS             6331..>6524
                     /locus_tag="PLC71_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRM02159.1"
                     /translation="MDYKKAGVDIEAGYKSVELMKEHVKRTMREEVLGGLGGFSGAFS
                     LKKIKDMDDPVLLSGTDGCGT"
BASE COUNT         2285 a          945 c         1525 g         1769 t
ORIGIN      
        1 gtggaatcgg tgcatgctta gcctgtgttt gcaagtcaaa agacgtagat tcccattcac
       61 atgttcataa caaacgtgtt tgcaaagatg ggccagtatt cgacgccagg gaggtggaac
      121 tataatgaat ttatcagttg atattgccgg agtaacaatg aagaaccctg tgacaacagg
      181 atcaggaaca tttggttccg gagaagaatt cagtgaattt gttgaccttt ccaaattagg
      241 agcagtgaca accaaaggag ttgctaatgt tccatggccg gggaattctg tgcctagagt
      301 tgcagaggta tatggtggta tgttaaatgc gatcggattg cagaatcctg ggatcgatgt
      361 gtttgttgaa cgagatattc catatcttaa gaaagccgga gcaacgatca ttgttaatgt
      421 ttgtggaaaa tccgaaaagg attatctcga ggttgtagaa agacttgctg accagccagt
      481 agatttatta gagatcaatg tatcttgtcc gaatgtgaaa gaaggcggga ttgcttttgg
      541 acaggatccc aaagcgttat atgatattac gaaggcagtg aaagcaaaag caaaacagcc
      601 gatcatcatg aagttaagcc cgaatgtcac agatatcaca gagatggcaa aagctgcagt
      661 tgatgcagga agtgatgctc tttctttgat taatacatta acaggaatga agatcgatat
      721 tgaaagaaga gattttgtgc ttgccaataa gacaggtggg atgtccggac cagcgatcaa
      781 accagttgct gtccgtatgg tatatcaggt tgctaacgct gtggatgtgc caattatcgg
      841 tatgggtgga atctcaacag ctgaggatgc attagagttt atcatggctg gtgcaacagc
      901 ggtatcagta ggaacagcaa actttatcaa tcctgcaacg acacttgaag tggttgatgg
      961 aatcaaaact tacatggaaa gacatcagat caatgatatt aacgaattga tcggatgtgt
     1021 caaatagata ataaaaggag gatgagggta tgaatatttt atttttcctg actccgaaaa
     1081 gtgaggttgc ctatatccct gccgatgaaa gtttaaggca ggcatttgaa aaactaaaat
     1141 atcatggata ttcagcagtg ccgacattaa ctgatgatgg taaatacgca ggaacgatca
     1201 cagaaggtga tgtattgcgt gccatggaat ctatgtgcca ttggaatata tcagaagctg
     1261 agaaagtcaa acttatggat gtaaagagaa agagaaacat tcaggcagtg acgatcagca
     1321 cagatatgga tgatcttgtc gatgtgatca cagatcagaa ttttgttccg gtcattgatg
     1381 atcaggagaa atttatcggg atcgtgacaa gaaaagatgt gattcgtttt tatcatgacg
     1441 aataccgcaa attagaaaag aatgaataag atggaggttt aaatagaaga tggaacagta
     1501 taagagagag tttatcgaat ttatggtaga aagtgatgta ttaaaatttg gagaatttac
     1561 attaaagagt ggaagaaaat ctccattttt catgaatgca ggagcttatg tgacaggatc
     1621 tcagttaaag agacttggag aatattatgc aaaagcaatc catagtactt atggagatga
     1681 ttttgatgtg ttattcggac ctgcatacaa aggaattcca ttaggagttg taacagcgat
     1741 cgcatacagc gaattatatg gaaaagaagt tcgttattgt tctgaccgta aggaagcaaa
     1801 agatcatggt gcagataaag gaaacttcct tggaagcgaa ttaaaagacg gtgaccgcgt
     1861 gatcatgatc gaagatgtta caacatctgg taaatccatg gaagagacag ttccaaaagt
     1921 aaaaggagca gcaaacgtag agatcgttgg attaatggta tccttaaacc gtatggaaat
     1981 cggtaaaggc ggagaaaaat gtgcactgga tgaagtgaaa gaattatatg gatttgatac
     2041 agcagcgatc gttgatatgg aagaagttgt agaatgttta tataacaaag aatgtaatgg
     2101 aaaagttgtg attgatgata cattaaaagc agcaattgat gcatactatg aacagtatgg
     2161 tgcaaaacat taaggatggt tcttgtgacg gttgagaaaa agaagttagc cagacgagga
     2221 ataaaagtta tattttatct ttatatcata ggtatatttt attttgtatt attgagtgaa
     2281 cgatatggga gggatacagg atatgatacc tcccatgtga acttagtttt attcaaagaa
     2341 atcaacaggt tctggacgta cagacatctt ttgtccaccg aagcagtggt cacaaatctg
     2401 tttggaaata tttttgcatt cagtccattt ggttttatga tcccgatcgt gatcaataag
     2461 aaaaaggcat tttttcgtgc agtgtttgca acttcatttt ttagtttagt gatcgagaca
     2521 agtcaattga tcatgaaagt aggggtattt gatgtagatg atcttctgat gaatacggtt
     2581 ggtggattga tcgggtatat gatatacagg gtcgtagtag cagtatatga tttatataac
     2641 agaaagaggg cggcaaaatg aagagaaaca aaagaaaaac atttgcatat cgtccatctg
     2701 acaagattat ttcacgggaa tcaattgttt ctgtgatttt aggagcagca gggatcattg
     2761 gatattacct gctgcttttc ggttccataa agacaggagg aacaagcagt ccattaagtg
     2821 gagctggagg atggatcttg ctggtagtat cagctatagg attatatttt ggaatacaaa
     2881 gtatgaaaga taccgcagca gtgatgaaat ggaagatcat tggatgtact tgcaacggaa
     2941 ttgtgctggc agtatctgtg atcatttttg tgattggaat aattgcaatg atctaagtaa
     3001 agaaaggaag agatgacttg aatacaatgt tatcagaaag acatgaatta aataagatcg
     3061 cactgttaga attaaaaaaa gatgtagaag aaacaagaga attttcttca tatttttcta
     3121 aaatggcaga ccttttatta tatgtaaatg agatttacga taccaaagag aggacattag
     3181 aacagttaaa acagtggaat agaatatggt atgaagagat cagccaggaa caatatgcac
     3241 atagttttgg gaatccagaa ttttgtacag aaaagtttgg aaaagagtat ggtcagattt
     3301 tcgcattttt atatgcagag atgcgcagtg gatttatcta tgcgatcgaa ggaagactgt
     3361 ttgaccttgt gatcaacaat gaattgttct tggaaatcag caatttattt ttgtcaggag
     3421 aagaacatcc gcagaagatc aagcatattg tttttgatca tatgagagat tacagcgagg
     3481 atgtttttga gtacagaatt agagaacagt tagacccaga acttgatttt gcaacaaaga
     3541 tcgtgatgga atctgatctc agtgatccag atattttgta tcaatatgga gaatatattt
     3601 ccgaaaatga aattgatact ttaaagtttt taaataaatt gccacaagaa gagatcacag
     3661 caatggccaa aacatatgtt gatggatttc atgaaggatt tatcatcaat aacattgata
     3721 tgagtccaaa acgaagtgtt aatatccgtt atacgatcgg atttgaacca gttgtcagag
     3781 aggcagttcg tttattctca gaaattgggc ttgcaccgat catctataga agtggcgtat
     3841 ctgttttaac acgaggactt cataagattg gttatgtttc atcttctcca aacccacagt
     3901 atgaatatga ccacagatat gatcaggcga tattctttga taatcgtttt atgaaacata
     3961 aattagaatg ttatcataat gcatatgaac attataaaga tgaagcgtat gtgtttgctg
     4021 gaccagcttg catggaaaca tttggggaaa tacctttttt accagaaaat aaagaagaga
     4081 atttaaaatt aagcaagaag cagcaggagc tttctgtgga atttcagatg aaagcctcag
     4141 agatcatgaa tcaatatatc aaaccagagc agaggagctt tacgatcatt tcatatccaa
     4201 ttcctgagat cggggataac tttgaagaga tttttaaaga agtcgtgaaa gttaatacat
     4261 tggataaaga caaatacaga cagattcagc agcatttgat cgatgccctg aatcaggcag
     4321 tggaagttca tatcaaagga aaaggaaaga ataagaccga cctatacgta gcgatgcatg
     4381 agatggatga cccatccaaa gaaacgaatt ttgaaaactg ccttgcagat gtcaatattc
     4441 cagttggaga agtgtttacc tcaccaaaat taacagggac caatggagta ctgcatgttg
     4501 gagaagtcta tttaaatgat ctgaaattca ttgatcttga aattactttt aaagatggaa
     4561 tgatttctga atatacatgc aaaaacttta aaacagagga agaaaataag gcatttatca
     4621 aagagaatct cttatataac agagatacgc tcccaatggg cgagtttgcg atcggaacaa
     4681 acacaacagc atatgtgatg gcgaagacat atgatatctt atatcagctt ccaatcctga
     4741 ttgttgagaa gatgggacct cattttgcag tgggagatac ttgctacagt cattgtgaag
     4801 aaactgaagt tcacaatcca gatggcaaag aaatcgttgc aaaatataat gaatgctcca
     4861 aagaaggcga gtatttccag tgtcacacag atattacgat tccatatgaa gaactggatt
     4921 ctatttgtgc gatcaccctt ggcggagatg agattttact gatcaaagat ggtaaatttg
     4981 tactttctgg tacagaaaca ttaaatgaac cattgacgga aatcggttaa atcagtacaa
     5041 tgataagaaa gataaaatcc tgaaagggaa gtttttgtta atttcataag ataaggagaa
     5101 agaacatggc aaaagtagca attgtaatgg gcagcgattc tgatatgcct atcatgaaaa
     5161 aagcagcaga gtttttagat cagatgggga tcgattttga gatgacgatc atttccgcac
     5221 acagagaacc agatattttc tttgagtggg caaaaggtgc gaaggaccgt ggagtgaaag
     5281 tgatcattgc aggtgctgga aaggcagcac atcttccagg tatgtgtgca gctttattcc
     5341 caatgccagt tatcggtatt ccgatgaaga cttccgacct cggtggtgtt gattctttat
     5401 attctatcgt acagatgcca tctggaatac cagtagcgac tgtagcgatc aatggaggac
     5461 ttaatgcagg aatccttgca gcgaagatcc ttgcaacatc agacgagaaa ttacttgaga
     5521 aattagaagc ttacagcgaa gagatgaaac agtcagttgt agagaaagca gagaaacttg
     5581 acaaagttgg atacaaaaat tatgaataag aagattcttg cagcgttttt atgcgtatgt
     5641 atggttgtag ttacaggaag ttttccagta agtgcagcaa aaacaaagaa gacatacaag
     5701 attgtaattg atgcaggaca ccagggaaag ggaaacagta agacagaacc tatcggaccg
     5761 ggcgcgaaga tgcgtaaacc caaagtagca tacggaacgc agggagtcaa aacgaaagta
     5821 ccagaatcca aattaactct gcagattgca ttaaagcttg agaaagagtt aaagaaacgc
     5881 ggttatgatg tgtatatgat aagaagaaaa cagaatgtaa acattagcaa taagcaaaga
     5941 gcgatccgtg caaataaaag tggtgctgat atctgtatca gactgcatgc ggatgcagca
     6001 tcagcgaatg ttcgtggtgt ttctgtgcta tacccatcac agcagaatcc ttatgtagca
     6061 aaattaagca agaagagtaa aaaactctca aagaatatac tggacagtta ttgtaagtca
     6121 accaaattca agaaacgtgg tttatcaaaa cgtgacgatc ttacaggaac aaactggagt
     6181 aagattccag tggcactgat agagatggga tttatgacaa ataagaccga agataaacag
     6241 atgcagaaga agcagacaca gaagaagatg gtagacggca ttgcaaatgg tattgatcaa
     6301 tattttaaat aatcaggagg agaagtaaca atggattaca agaaagcagg cgtggatatt
     6361 gaagcaggat acaaatctgt agaactgatg aaagaacacg taaagagaac catgagagaa
     6421 gaggtacttg gtggattagg aggattttcc ggagcatttt ctttaaagaa gatcaaagat
     6481 atggatgatc cagtcttact ttcaggaact gatggatgtg gaac
//
LOCUS       DAOMUE010000097         6512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3192_length_6512_cov_2.257550, whole genome shotgun sequence.
ACCESSION   DAOMUE010000097 DAOMUE010000000
VERSION     DAOMUE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3192_length_6512_cov_2.257550"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>248)
                     /locus_tag="PLC71_05650"
     CDS             complement(<1..>248)
                     /locus_tag="PLC71_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRM02160.1"
                     /translation="RDQFIVDAIQGGAGTSANMNANEVIANRAIEILGGTKGDYSIVH
                     PNDHVNMSQSTNDVIPTAGKITVLKLLPQTIKELEKLE"
     assembly_gap    249..275
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<276..461)
                     /locus_tag="PLC71_05655"
     CDS             complement(<276..461)
                     /locus_tag="PLC71_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HRM02161.1"
                     /translation="MLSRMEHDSIGALNVPEEAYYGVQSIRAATNFQITHRPLHPVLI
                     DSIVMVKKAAAITNEKSG"
     gene            637..961
                     /locus_tag="PLC71_05660"
                     /pseudo
     CDS             637..961
                     /locus_tag="PLC71_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355935.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
     gene            complement(1056..1937)
                     /locus_tag="PLC71_05665"
     CDS             complement(1056..1937)
                     /locus_tag="PLC71_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02162.1"
                     /translation="MEVNVEIVLSYNDEETDWKVKKNISKFIYRLKKYRTGNRFSGEY
                     TYKINQDSELYKQIIEFYKSNRKDVEFICLDYDVKVSDEEFEKAKAFVLCFPEYYCEE
                     YEDIENEYSECESCHSKEKTNSLFYAQPKGYIKKHENDYGFAGLDGTGELLLLPKLVE
                     KLKKSGVDKKYFQPIISKSKKILGYTFITDNILPQKSYIDENYKFENQCEKCGRINMT
                     ENENIFYFIPKRITEEGIKNLKDVNKTYEFYDEYREIVINKKVAQIIKENVPYVKFYP
                     VILDNRNLNSRRPFKIS"
     gene            complement(1927..2481)
                     /locus_tag="PLC71_05670"
     CDS             complement(1927..2481)
                     /locus_tag="PLC71_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02163.1"
                     /translation="MIVCNIQLQNKKDSFIKKSYSIPVGIFPGNKATIDDKGNIYVAT
                     GMLGVVQKFNKNGDFIKSILIKDDIDMIKNERDGIHVYAGKFDYTDYHITEKKIIEKE
                     ISETDLDHIKSGPNELVIGNFWIFKGKITILKSSLFPIDPFIIMYTAILMEIIFWIKA
                     KISRFRENKSSKSVVITGGDEIGS"
     gene            complement(2505..3059)
                     /locus_tag="PLC71_05675"
     CDS             complement(2505..3059)
                     /locus_tag="PLC71_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02164.1"
                     /translation="MMHGLKQLVYDNQPFFSSNQTGKTNRELYILWKQNKRDDIFLLL
                     YTQLWMVLEEVYMENVIDYYKKDCYEKKLFFELVEYQKRFFKKNIKIQLLTGYFYATT
                     EYLFFLNKENDIYNIVDEGKNIIKKLYDNNQEKFEVYIFYICILQGKRKKWIKKRKWY
                     LREIKKLFPSNSEIDQYFREIFLL"
     gene            complement(3028..3375)
                     /locus_tag="PLC71_05680"
     CDS             complement(3028..3375)
                     /locus_tag="PLC71_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 35 domain-containing
                     protein"
                     /protein_id="HRM02165.1"
                     /translation="MVSVAMGASSSSGLAVKGVNSAIRRVASDQNKVRHIMQSKHAWT
                     KVTKKNQWKYVKPIVKKAMKSGKMEAIGKTKGKEIVYKFVYNYKGKIIEGTCIAKKGV
                     VKLSDAWVKTIGL"
     gene            complement(3341..3952)
                     /locus_tag="PLC71_05685"
     CDS             complement(3341..3952)
                     /locus_tag="PLC71_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRM02166.1"
                     /translation="MIGTEGNVLATERFKGDDTQYYLYNEDIQGSTTSLVKEDGSADA
                     IYQYTDFGETIIHGDDQAKNEVCYTGGIYDQSTGLYYLNARYYNPEDGRFMTEDTYRG
                     DTTKSETGHLYVYCANNPVNYVDPSGHFLVSTAVLVGVGVGGIVGAIAGSYKGRLVAK
                     RLGYKGKKRNLFIATYGIKGAAVGAIIGAFAGYGIGCYGSIIK"
     gene            complement(4237..4494)
                     /locus_tag="PLC71_05690"
     CDS             complement(4237..4494)
                     /locus_tag="PLC71_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02167.1"
                     /translation="MESRLCRKNGTIIKREKGKKGYYMTDNWYLYKYNKDVRKVLQEY
                     PNIYAFADWIDLCFVSENKIILQTIAHEGMCFGSAELFRDI"
     gene            complement(4506..4673)
                     /locus_tag="PLC71_05695"
     CDS             complement(4506..4673)
                     /locus_tag="PLC71_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02168.1"
                     /translation="MVYEDICNFGMDQLVITQYKKILKILQENSNILILKIPIEKVKS
                     KEEKEREDLKN"
     gene            complement(4690..4911)
                     /locus_tag="PLC71_05700"
     CDS             complement(4690..4911)
                     /locus_tag="PLC71_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02169.1"
                     /translation="MRLKANGNVFVTSSGYAYDACKAASPIHKVSAMQKHKKEKDQGK
                     QYYHYHPMLKWSKNKNARKQMHVHCWFLK"
     gene            complement(5481..6005)
                     /locus_tag="PLC71_05705"
     CDS             complement(5481..6005)
                     /locus_tag="PLC71_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02170.1"
                     /translation="MIYELKFSYYEDNSENNDEIIIAVYSSYKKAEIGKKKFSQQPRF
                     KGKEEFLEINEYKINEAEWNEGFWRATKSYFIIKLLNNYMIEKEEEYNPENHDIEVKY
                     GENIKVYNGKMDYYYDHEKFLCLKNTENGQMYCLDKQQRKILYKINKYEDMLKFLNQK
                     YEIENFGWKNIFRE"
     gene            complement(6230..>6512)
                     /locus_tag="PLC71_05710"
     CDS             complement(6230..>6512)
                     /locus_tag="PLC71_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRM02171.1"
                     /translation="VYCANNPVNYVDPSGHFAAAMYTVIKLVLVGGAVAYVSYSSWKN
                     HSSKTKYAGRKIISKTKVSVWLTAGVVDRIIYSKSKKKVILKRRKNQKK"
BASE COUNT         1856 a         1244 c          702 g         2683 t
ORIGIN      
        1 ttctaatttc tcaagttctt tgatcgtttg tggaagtaat tttaagacgg tgatctttcc
       61 tgccgttggg attacatcgt tcgtagactg tgacatattg acatgatcgt ttggatgtac
      121 aatgctgtaa tctcccttgg ttccaccaag gatttctatc gcacgattgg caatcacttc
      181 gtttgcattc atattggcag aagttccagc ccctccttgg atcgcatcca cgataaactg
      241 atctcttann nnnnnnnnnn nnnnnnnnnn nnnnntccag acttttcatt cgtgatcgct
      301 gctgcttttt tgaccattac gatgctgtca atcaacactg gatgtaatgg acggtgtgtg
      361 atttgaaaat ttgtggctgc acgtatactc tgaactccat agtatgcttc ctctggtacg
      421 tttaatgctc caattgaatc gtgttccatt ctgcttaaca ttgtcttcct cttttctgat
      481 tactcatgct gtcgttttgt ttcaagattt tatgtgttgg agcttaacac agtatgcaat
      541 gttcgtaaac cttaatcctg tctttccaaa tttaaagttc aaattttgtg tttcatcttt
      601 tcttttttta atgaaagtct tgatttactt taaataatta agcggttgga agcggaagga
      661 attctgactg gatttcatgc ttctgttgac ccgatccaac ttggttatca tattaaagca
      721 tatatcagtc ttgagatcag ccctgcacaa aaacccgaag tttatccttt tcttcgagca
      781 caccccaaat gtattggaat gtgattgtgt aactggaaat tattctgtgc tactgaaggt
      841 tgcttttcct agtacgatgg aactggatga atttattggg aaattgcagc actttgggaa
      901 gactagtacg atgatcgtgt tttctacgat cgttgagaat cgtgtggtgg ttcctgaata
      961 gttgatttgt ttttatttta tcctagatat cccaaatgaa tcatatttat ctctatgtac
     1021 aagaacttaa tagaccacat tgagtacctt agaccttacg atatcttgaa aggtctgcga
     1081 gagtttaaat ttctgttatc taaaataaca gggtaaaatt ttacatatgg aacattttct
     1141 tttataattt gtgcaacctt tttatttatt acgatttccc tatactcatc gtaaaattca
     1201 tatgtcttat ttacatcctt taaatttttt attccttctt ctgttattct tttcggtatg
     1261 aaatagaaaa tgttttcatt ttctgtcata tttattcttc cacatttttc acattgattt
     1321 tcaaatttat aattttcatc aatataactt ttctgtggta atatattatc agtaataaaa
     1381 gtatatccta atatcttttt acttttgctt ataatcggct gaaaatattt tttatctact
     1441 cctgattttt ttaatttctc caccagtttt ggtaataaca acaactctcc tgttccatcc
     1501 aatcctgcaa acccataatc attctcatgt ttttttatat atcctttcgg ttgtgcataa
     1561 aataagctat ttgttttttc tttactatga caactttcac attcactata ttcattttca
     1621 atatcttcgt attcttcaca gtaatattct ggaaaacata aaacgaatgc ttttgccttt
     1681 tcaaattctt catctgaaac tttaacatca taatccaaac agataaattc tacatctttc
     1741 ctatttgatt tataaaattc aattatttgt ttatatagtt ctgaatcttg attaatctta
     1801 tatgtatatt ctccagaaaa tcgatttcct gttctgtatt tctttaatct gtatatgaat
     1861 ttagaaatat ttttttttac tttccaatct gtttcttcat cattataact taatacaatt
     1921 tctacattaa cttccaattt cgtctcctcc tgttatcact acacttttgc tgcttttgtt
     1981 ttctctaaac cgacttatct ttgcttttat ccaaaatata atttccatca atatagctgt
     2041 atacataata ataaaaggat ctattggaaa taaactactt ttcaagattg taattttacc
     2101 tttgaaaatc cagaaattac caatcaccaa ttcattgggt cccgatttta tatgatctaa
     2161 atctgtttca cttatttctt tttctattat ttttttctct gttatatgat aatccgtata
     2221 atcaaacttg cctgcatata catgaattcc atctctttca ttcttaatca tatcaatatc
     2281 atccttaatt aagatactct taataaagtc gccattcttg ttaaatttct gaacgacacc
     2341 taacattcca gtcgcaacat atatattccc tttgtcgtca atggttgctt tattccctgg
     2401 aaaaatacca acaggtatgg aatatgattt tttaataaaa gaatcttttt tattttgtaa
     2461 ttgtatatta catactatca ttgttacaaa gtctttaaca ttacttacag taaaaatatt
     2521 tcccgaaaat attgatctat ttccgaatta cttggaaata gttttttaat ttctcgtaaa
     2581 taccattttc ttttttttat ccattttttt ctctttcctt gtaaaatgca aatataaaaa
     2641 atatatactt cgaatttttc ttgattatta tcatatagtt tttttattat attttttcct
     2701 tcatcaacta tattataaat atcattttcc ttatttaaga aaaataaata ttccgtggtc
     2761 gcataaaaat aacctgtaag taattgtatt ttgatatttt ttttaaaaaa ccttttttga
     2821 tattctacta attcaaaaaa aagttttttt tcataacaat ctttcttata ataatctata
     2881 acattctcca tataaacttc ttctaatacc atccaaagct gtgtatataa aagtaagaaa
     2941 atatcatctc tcttattttg tttccacaaa atatataact ctcgattcgt ttttccagtt
     3001 tgatttgaag aaaaaaatgg ttgattatca taaaccaatt gttttaaccc atgcatcact
     3061 taattttaca actcctttct tagctataca tgtaccttcg ataattttcc ctttataatt
     3121 gtatacaaat ttatatacaa tttcttttcc tttagttttt cctatagctt ccatttttcc
     3181 acttttcatt gcctttttta caattggttt tacatacttc cactgattct tttttgttac
     3241 ttttgtccac gcatgtttac tctgcataat gtgccttact ttattttgat cagaagctac
     3301 tcttcttatt gcactattga ctccttttac cgctaatcca ctacttgatg atgctcccat
     3361 agcaaccgat accataacct gcaaatgctc ctataatggc tcctactgca gctcctttaa
     3421 ttccatatgt tgctataaat aaatttcttt ttttcccttt atatcctaat cttttagcaa
     3481 ctaatctacc tttatatgaa ccagctattg caccaacaat tcctcctact ccgacaccta
     3541 ctaaaactgc tgtacttact aaaaaatgcc cactaggatc cacataattc accggattat
     3601 tcgcacaata cacatataaa tgtcccgtct ccgattttgt cgtatctcca cgataggtat
     3661 cctctgtcat aaatctacca tcttctggat tataataccg tgcattcaga taatacaatc
     3721 ccgtactctg atcatagatt ccaccagtat aacacacttc atttttcgcc tgatcatctc
     3781 catgaataat tgtctctcca aaatccgtat actgatagat tgcatctgca cttccatctt
     3841 ctttcactag gctagttgta cttccctgaa tatcttcatt atacagataa tattgcgtat
     3901 catccccttt gaatctctca gttgcaagga catttccttc tgttccaatc aagttctgtg
     3961 taagtcatct cttttacacg gtcaaaactg tcataggtat agacttttta aactgcctga
     4021 tcactgttat ttaatagatt tctgtggtct ttaatctctt tgacttttcc ataagcatca
     4081 taagtgtaac ttctgtgact tcgtaaggtc tcagggaact taagttatga aatctcaagt
     4141 tactcaaact ttgtcttaat gtccctggga acttaagtaa cttataatat aatgcattta
     4201 aggtacctca gaccttacga tgtctgctgg ttgatatcat atatctctaa acaattcagc
     4261 tgatccaaaa cacattcctt catgtgctat tgtttgtaaa ataattttat tttctgagac
     4321 aaaacataaa tctatccagt ctgcaaaagc ataaatatta ggatattctt gtaaaacttt
     4381 tcttacatcc ttgttatatt tatataaata ccaattatcc gtcatataat atcccttttt
     4441 tcctttttcc cttttaatta tagttccatt tttacgacat aatctacttt ccaagaaaaa
     4501 tttttctaat tttttaaatc ttccctttct ttttcctcct tactttttac cttctcaatt
     4561 ggtattttta atattaaaat attactattt tcctgaagta tttttaatat ttttttatat
     4621 tgggttatta ctaattggtc cataccaaaa ttgcaaatat cttcatacac cataattatt
     4681 aaaatcctcc tattttaaaa accaacaatg tacatgcatt tgttttctcg catttttatt
     4741 tttactccac tttaacattg gatgataatg ataatattgt tttccctgat ctttttcctt
     4801 cttgtgtttt tgcattgcag atactttatg aattgggctt gcagctttac atgcatcata
     4861 agcatatcca gaagatgtca caaaaacatt tccatttgct ttcaatctca tagctgcttt
     4921 agatttagtc tttatagcat catatatata tactttcgca tttatccttt ttgctttata
     4981 ataatgtatc ttaggttgtt ttcgctttag tgttgaaatt gcagttgcag caacatatgt
     5041 aattccagct attacaatat atttaagcgc ctttaaaata actcctccta agtctttctt
     5101 aaggtctctg ggaccttaag gatttttact attcagacaa gattttttag aatacatgaa
     5161 actccaaaat gcgtatacta taattccagc agtagtcaaa atgaaatgaa tagtctgtaa
     5221 cgacaaaaaa cgaagacatt gataccgtag accttaagaa attttaagat tctgtgatac
     5281 ctataaaaaa taataattat ccttatttac tttcattttt tttataataa aacaattcgg
     5341 atattcttta aatcctggaa gtgttgatct atattttttc attacacttc gtgctttttt
     5401 ccaacttcca aaataaccaa tcataagtaa actaccctca tcttcatatg tcagttgata
     5461 tatatacata tttaaccacc tcactcccta aaaatatttt tccatccaaa gttttcaatt
     5521 tcatattttt ggtttaaaaa tttcaacata tcttcatatt tatttatttt atatagaatc
     5581 tttctttgtt gtttatctaa acaatacatt tgtccattct cagtattttt taaacacaaa
     5641 aatttttcat gatcataata ataatccatt tttccattat acacttttat attttctcca
     5701 tattttactt ctatatcatg attttctgga ttatattctt cttctttttc gatcatataa
     5761 ttatttaaaa gttttattat aaaatatgat tttgttgctc tccaaaaacc ttcattccac
     5821 tctgcttcat tgattttata ttcattaatt tccaaaaatt cctcttttcc tttaaatctt
     5881 ggttgttgtg aaaatttttt ctttcctatt tctgcctttt tatatgatga atataccgca
     5941 ataattattt catcattgtt ttctgaatta tcctcataat acgagaattt caactcataa
     6001 atcatgaata tatttgcacc tccatttata tttttactgt atcaattatc gtcctctatc
     6061 tccccacttt ctcaattcac tatattcctt tccttgcttt ccttttcttg tccattttcc
     6121 ttttccataa tgttcattca ttttcctaat tgcatactca tttccacttt cccctttttt
     6181 tggtctagat ccatgtgccc aacttggaat atctgtcgct ctttcttttt tacttttttt
     6241 gattctttct tctttttagt attacttttt tcttactttt actatatatt atcctatcca
     6301 ctactcctgc tgtaagccat acagatactt ttgttttaga aataattttt cttcctgcat
     6361 attttgtttt tgaactatga tttttccatg atgaatatga cacataagct accgcacctc
     6421 caacaagtac taattttata actgtataca ttgctgccgc aaaatgccca ctaggatcca
     6481 cataattcac cggattattc gcacaataca ca
//
LOCUS       DAOMUE010000098         6467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3228_length_6467_cov_2.718964, whole genome shotgun sequence.
ACCESSION   DAOMUE010000098 DAOMUE010000000
VERSION     DAOMUE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3228_length_6467_cov_2.718964"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLC71_05715"
     CDS             <1..555
                     /locus_tag="PLC71_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM02172.1"
                     /translation="TTAELSELRSDKIKKINLFLDETDIQFIGNILKEDQKDEIDYRK
                     IFRKELFFYDVEFKNKEEILEHMTREMQIRGLISKEGSKSVFKREEMSTTELGNMVAI
                     PHAMSNDSEEAVVSVMILKKPILWENEKVQVVLLLNVPKSQYNMWEVVFKRLYQYLIG
                     DQGVTKLIKDKDYDDFIRHLERNE"
     gene            568..1005
                     /locus_tag="PLC71_05720"
     CDS             568..1005
                     /locus_tag="PLC71_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM02173.1"
                     /translation="MESLVLNKDNVKLKVKASDWEEAVRIGAGMLVEQGCAKQSYVDG
                     IIASVKELGPYIVIADGIAMPHTRPEQGAIGIGCSLITLDQPVKFDGDDSEVKVMICF
                     SAVDSESHMDILKMIVEFVERGLIDDIAKATTYEELLEVIKEK"
     gene            1032..1322
                     /locus_tag="PLC71_05725"
     CDS             1032..1322
                     /locus_tag="PLC71_05725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM02174.1"
                     /translation="MLQILTVCGAGLGSSFACQMSVDAVLKELGVEAKLDHTDISSVG
                     GAKADIILSGKNFEKQFERYTLDCPTIFLDRLVDKNEIKEKLTPVLKDLGVL"
     gene            1353..2630
                     /locus_tag="PLC71_05730"
     CDS             1353..2630
                     /locus_tag="PLC71_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002607355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HRM02175.1"
                     /translation="MQVLNFIIKNILTQASVVVGLIALLGLVLQKKPIGTVVSGTMKT
                     ILGFLVLSAGSSVIQGSLAIFGDLFNKAFGLKGLVASIEGINGQAMTDLGLGSEIAIT
                     LAGIFIVNIILARITPFKYIFLTGQALLWESTLCVVFAYFCGLRGLPLIIVGSIVGGA
                     FATLMPAFAQPVMRQITGSDDIALGHFCTFGYMFTALIAKLTGDKSKSTEDVALPKSL
                     EFLQDTYLSVMVVMVPFYLIVAIFAGPKACAEYAGATNYIVFMFLQALQFVTGLYVLM
                     AGVRLLLAELVPAFQGISMKLVPNSKPALDCPVLFPYAPNAVILGFIFTTIGSIIGMF
                     LTPMLGLPMILPGVMSNFFAGGTAGIFANQVGGRRGTIIGCIAHGIFIMILPALLSPM
                     LGQIGFQNMTCTDVDTVVTGFFFMIIKSIAGIF"
     gene            2681..3064
                     /locus_tag="PLC71_05735"
     CDS             2681..3064
                     /locus_tag="PLC71_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRM02176.1"
                     /translation="MFFFGKKKKEKKQEQAVPVVNKEELIAKASEKVTELESKTGDER
                     VSLLNEIGSLYTQAEETDLAIQYYEESLELCKQHGKASTDLMKLYNKKRQEAAEAKDD
                     EKVKEYMDKVQNLMQMSKDMIRGNA"
     gene            3144..3989
                     /locus_tag="PLC71_05740"
     CDS             3144..3989
                     /locus_tag="PLC71_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRM02177.1"
                     /translation="MYKNLIELFKENEGKGAVGAFNLHCFEMLPAMIQAAEELNVPII
                     IQTSLGTAEYIGFEPLIAAVKALAEKSSVNVALHMDHCKSIEALKKAIDLGYSSVMYD
                     GSALPIEENIKNTQEVVEYAHAHGVSVEGEIGSIGGAEEGVVVEKDAAMYTKPEDALH
                     YVNETHVDALAVSIGTTHGQFKSKAKINYELLTELKAKLGPVGLVLHGGTGVSDEDMK
                     RCVREGMKKINVGTELNKNYIEVVSKTFTADDVTPLTSLRNLLGPANERIKEIVIDKA
                     SLFKL"
     gene            4113..4721
                     /locus_tag="PLC71_05745"
     CDS             4113..4721
                     /locus_tag="PLC71_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM02178.1"
                     /translation="MKKTIVLLAGYPATGKSYLCNKILEKLPDFLIVSQDDMKEKLFD
                     QYGFDNMEEKVAIENQSWEQYYEVMEEDLKNGDSIISDYPFSVKQKDKIKNLAEKYEY
                     QVVTFRLIGDLEVLFKRSQKRDLDPKRHLSHLVSRYHKGDVLEDRSKADCLVTYDIFM
                     DRCKNRGYGTFELGHLIEVDVTDFSKIDYPALIKELCDLVEE"
     gene            4733..5662
                     /locus_tag="PLC71_05750"
     CDS             4733..5662
                     /locus_tag="PLC71_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRM02179.1"
                     /translation="MGSIFCIGQSAYDITIPLDGAICENQKYRVTQKEECGGGPAFNA
                     AYLCAMWGEKVELISRIGNDTYGKRLLEIAAQAGIGTNYLIKDDEIETPHSYIFSNQE
                     NGSRTLFNFPGEKKDIEYKYPDEEVSVILSDGHEPEISVEAIHHYKSAISIVDAGTCR
                     ESTMQVAKEVDYLVCSEDFARQYTGKTINLNDPKKACEIFEEVEQINHKHAVVTLGEK
                     GLLYRRDGKITLMPAFKVKAVDTNGAGDIFHGAFAYAIKQQLDFYDVLKISSMASAIS
                     VQTLGAQSSIPQLTKVTSELQKKGNHYDKRRYL"
     gene            5640..6293
                     /gene="deoC"
                     /locus_tag="PLC71_05755"
     CDS             5640..6293
                     /gene="deoC"
                     /locus_tag="PLC71_05755"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRM02180.1"
                     /translation="MTREDIFKTVDHTLLAQTATWEEIKEICDDAIKYQTASVCIPAS
                     YVKRAKDYVDGKMAICTVIGFPNGYSTTAVKEYETKDAILNGADEIDMVINLGDVKDK
                     HFDKIKGEIALLKSACGDKILKVIIETCLLTEEEKIKMCEIVTEAGADFIKTSTGFST
                     SGATFDDIALFKKYVGENVKIKAAGGISSFDDAKKFIELGADRLGTSRLVKIAKAEA"
BASE COUNT         2351 a          952 c         1386 g         1778 t
ORIGIN      
        1 acaacggcgg aactttcaga acttcgatct gacaaaataa agaaaataaa cctatttctg
       61 gatgaaacag atattcagtt cataggaaat attttaaaag aagatcaaaa agatgaaatt
      121 gattatcgaa agattttccg aaaagaatta tttttctatg atgtggaatt taagaataaa
      181 gaagaaatcc ttgaacatat gacaagagag atgcagatac gaggattgat cagtaaagaa
      241 ggaagtaaat ccgtatttaa aagagaagaa atgtctacga cagaattggg caacatggta
      301 gcaataccgc atgcaatgtc aaatgactct gaagaggcag tcgtatccgt tatgatctta
      361 aagaaaccaa tcctctggga aaatgaaaaa gtgcaggttg tacttttact caatgtacca
      421 aagtcacaat acaatatgtg ggaagttgta tttaaacgat tatatcaata tctgattggt
      481 gatcaagggg ttacaaaatt aatcaaagat aaagattatg atgattttat tcgtcatttg
      541 gaaagaaatg aataggaagg aaatatcatg gaatcactag tattaaataa agacaacgtt
      601 aaattgaagg taaaagcttc tgattgggaa gaagcggtta gaattggggc agggatgctt
      661 gtagaacaag gatgtgcaaa acaatcttat gttgatggta tcattgcttc tgtcaaagaa
      721 ttagggcctt atattgtcat tgcagatgga atcgcaatgc cgcacacaag accagaacag
      781 ggagctattg ggatcggttg tagtctgatc acactggatc agccagtaaa atttgatgga
      841 gatgacagtg aagtaaaagt tatgatctgt ttttcagcag tagacagtga aagccacatg
      901 gatattctta agatgatcgt tgagtttgta gagcgcggac tcattgatga tatagctaaa
      961 gcaacgactt atgaagagtt acttgaagtg atcaaagaga aatagtttta aaaaagaaag
     1021 gagacgaaac tatgttacag attttaacag tatgcggagc aggactggga agcagttttg
     1081 catgtcagat gagtgtagac gccgtattaa aggaactggg tgtggaggca aaacttgacc
     1141 atacagacat ctctagtgta gggggagcaa aggctgacat cattctttca ggaaagaact
     1201 ttgagaaaca gtttgagcgt tatacactgg actgtccaac aatttttctc gatcgattgg
     1261 tcgataaaaa tgagatcaaa gaaaaactga caccggtttt aaaagatctt ggagttttat
     1321 aaaactagca aaatagagag tgaggagaaa aaatgcaggt tttaaatttt atcattaaga
     1381 acatcctgac acaggcatct gttgtcgtag gactgatcgc actgcttggt ttagtgttac
     1441 agaaaaaacc aattggtact gttgtatcag gaacaatgaa aactatctta ggattcttag
     1501 tattatccgc aggttcaagc gttatccagg gatcattagc tatttttggt gatctgttta
     1561 ataaggcctt tggacttaag ggtcttgtag catctatcga aggtattaac ggtcaggcaa
     1621 tgacagacct tggacttgga tcagagatcg ctatcacatt agcaggtatc tttattgtta
     1681 atattatttt agctcgtatt acaccattta agtacatctt cttaacagga caggctttat
     1741 tatgggaatc tacactttgc gtagtatttg cttacttctg tggattaaga ggacttccat
     1801 tgatcatcgt aggatcaatc gttggtggtg cattcgcaac attaatgcca gcattcgcac
     1861 agccagttat gcggcagatc accggtagtg atgatatcgc attaggacat ttctgtacat
     1921 ttggatatat gttcacagca ttaattgcta aattaacagg tgataaatct aaatcaacag
     1981 aagatgttgc attacctaag agtcttgaat tcttacagga tacatattta tcagtaatgg
     2041 tagttatggt gccattttac ttgatcgtag caatctttgc aggacctaaa gcatgtgctg
     2101 aatatgcagg agctacaaac tacatcgtat tcatgttctt acaggcatta cagtttgtaa
     2161 caggtttata cgtattaatg gccggtgttc gtttactgtt agctgaatta gtaccagctt
     2221 tccagggaat ctctatgaaa ctggttccaa attcaaaacc agccttagac tgcccagtat
     2281 tattcccata tgcaccaaac gcagtaatct taggattcat ctttacaaca atcggatcta
     2341 tcatcggtat gttcttaaca ccaatgcttg gattaccaat gatcttacct ggagttatga
     2401 gtaacttctt cgcaggtggt acagctggta tctttgctaa ccaggttggt ggacgtcgcg
     2461 gaacgattat tggatgtatt gcacatggta tcttcatcat gatcttacca gctttacttt
     2521 ccccaatgct tggacagatt ggattccaga acatgacatg tacagacgta gatactgtag
     2581 taacaggatt cttcttcatg atcattaaat ctatcgcagg aatcttctaa gattagaaag
     2641 caaaagatca ttatagaaag ttatacagga gggatgatca atgtttttct ttggtaaaaa
     2701 gaagaaagaa aagaagcaag agcaggcagt tcctgttgta aataaagagg aattgatcgc
     2761 gaaagcttca gaaaaagtaa cagaattaga aagtaaaaca ggggatgaga gagtttcatt
     2821 attaaacgaa atcggatccc tgtatacaca agcagaagaa acagatctgg caatccaata
     2881 ttatgaagaa agcttggaac tttgcaagca gcacggaaaa gcgagcacag atctaatgaa
     2941 actttacaat aaaaaacgtc aagaagctgc agaagcgaaa gatgatgaaa aagtaaagga
     3001 atatatggat aaggttcaga acctgatgca gatgtcaaag gatatgatcc gaggaaatgc
     3061 ataacattta cggggaggaa attcctccct gcaaacatat aattcattcg caacgtataa
     3121 ggcaaaatta aaggagtaaa gaaatgtata aaaatttgat tgaattattt aaagaaaatg
     3181 aaggtaaagg agcagtagga gcattcaact tacattgttt tgaaatgctt ccagcaatga
     3241 ttcaagcagc agaagaactg aacgttccaa tcattatcca gacttctctt ggaacagctg
     3301 aatatattgg ttttgaacca ttgatcgcag ctgtaaaagc tttagcagaa aaatcttctg
     3361 taaatgtagc tcttcatatg gatcattgta aaagcattga agcactgaaa aaagcaatcg
     3421 atcttggata ttcttctgta atgtatgatg gatctgcatt accaattgaa gaaaatatca
     3481 aaaatacaca agaagttgta gaatatgctc atgcacatgg agtatctgta gaaggtgaaa
     3541 tcggatctat cggtggagct gaagaaggtg ttgtagtaga aaaagacgct gcgatgtata
     3601 caaaaccaga agatgcactt cattatgtta atgaaacaca tgtagatgca ttagcagtat
     3661 ctattggtac aacacatggt cagttcaaat ctaaagctaa gatcaattat gaattattaa
     3721 cagaattaaa agctaaatta ggacctgttg gattagttct tcatggagga acaggagtat
     3781 ctgatgaaga tatgaaacgt tgtgtacgtg aaggaatgaa aaagatcaac gtgggaacag
     3841 aattaaataa aaactatatt gaagttgtca gcaaaacatt cacagctgat gatgtaacac
     3901 cactgacatc tttaagaaat ctgttaggac cagcaaatga aagaatcaaa gaaattgtta
     3961 tagataaggc ttcattattt aaattatagg attaaaaagt ttaaataacc ctcttatatt
     4021 atataattca tcaggtgcgg cagggaggaa tgaatccctg ccgcatttaa ctttttagaa
     4081 aaatatctat catttgagcg gaggaaataa gcatgaaaaa aacgatcgta ttgcttgcag
     4141 gatacccggc aacaggaaaa agttatcttt gtaataaaat attagaaaaa cttccagact
     4201 ttttgatcgt atctcaggat gatatgaaag aaaaattatt tgaccagtat ggatttgata
     4261 atatggaaga aaaagtagcc attgaaaatc agagctggga acagtactat gaagttatgg
     4321 aagaagatct taagaatggg gattccatta tttcagatta cccattcagt gtaaaacaga
     4381 aagacaagat taaaaatctt gcagagaaat atgaatatca agttgtaaca ttccgcttga
     4441 tcggagatct agaagtttta tttaagcgtt cacaaaagag agatttggat ccaaagagac
     4501 atttaagtca tctggtatca cgttatcata aaggtgatgt actagaagac agaagcaagg
     4561 cagattgtct tgtaacctat gatattttta tggatcgttg caagaaccgt ggatacggaa
     4621 catttgaact gggacatctg atcgaagttg atgtaacaga tttttcgaag attgattacc
     4681 cagcattgat caaagaatta tgtgatcttg tagaagaata aaggagaaac agatgggaag
     4741 tattttttgt atcggacagt cagcgtatga tatcacgatt ccgcttgacg gcgcgatctg
     4801 tgagaatcaa aaataccgcg tgacacaaaa agaagaatgt ggaggcggac ctgcatttaa
     4861 tgctgcatat ctgtgtgcaa tgtggggaga aaaagtagaa ttgatcagca ggatcggaaa
     4921 cgatacttac gggaaacgat tgttagaaat tgctgcacag gctgggatcg gaaccaatta
     4981 tctgatcaag gatgatgaga ttgaaacacc gcatagttat attttttcaa atcaggaaaa
     5041 tggatcaaga acgttattta atttcccagg tgagaaaaaa gacattgaat ataaatatcc
     5101 ggatgaagaa gtctctgtta tcttaagtga tggacacgaa ccagagatca gtgtggaggc
     5161 aattcatcat tataagagtg ccatttcaat cgtagatgca gggacatgca gagaaagtac
     5221 aatgcaggta gcaaaagaag tagattatct ggtatgttct gaagattttg caagacaata
     5281 tacaggaaag acgatcaatc ttaatgatcc aaagaaagca tgtgaaatct ttgaagaagt
     5341 agaacagatc aatcataagc atgcagtggt cacactggga gaaaaaggat tattatatcg
     5401 tagagatgga aagatcacat tgatgccagc atttaaagta aaagcagtcg atacaaatgg
     5461 tgcaggggac atttttcatg gtgcatttgc atatgcgatt aagcagcaat tggattttta
     5521 tgatgtatta aaaataagct ctatggcgtc tgcgatttcc gtgcagacat taggagcaca
     5581 atcctccata ccacaattaa caaaagtaac aagcgaactt caaaagaagg gaaatcatta
     5641 tgacaagaga agatatcttt aaaactgttg atcacacact attagcccag acagcaactt
     5701 gggaagaaat taaagaaata tgcgatgatg cgatcaaata ccagacagca tctgtatgta
     5761 ttccagcatc ttatgtaaaa agagcaaaag attatgttga tgggaaaatg gcaatctgta
     5821 cagtgatcgg attcccaaat ggatatagta caacagcagt caaagaatat gaaacaaaag
     5881 atgctatatt aaatggtgca gatgaaattg atatggtcat caacttagga gatgtcaaag
     5941 ataaacattt cgataagatc aaaggcgaaa ttgcgttatt aaagagcgca tgtggagata
     6001 aaatattaaa agttatcatc gaaacatgtc tgttaacaga agaagaaaag attaagatgt
     6061 gtgagattgt aacagaagca ggagcagatt tcatcaaaac atctacagga ttctcaacat
     6121 caggagcaac atttgacgat attgctttat ttaagaaata tgttggagaa aatgtaaaga
     6181 tcaaagcagc aggaggaatc agttcctttg atgatgctaa gaaatttatc gaacttgggg
     6241 cagaccgcct tggaacaagt cgattagtaa aaatcgctaa agcagaagct taaaatacaa
     6301 tttgagaata tttatcgtta acaaaaagga atgttgcatt cgtgcaacat tccttttatc
     6361 atttttatat agaatgatga gaagaggaac aatatatgtc aaaagagata tcaaaacagg
     6421 aaaaagaaga ttatctaagt cttatgtatg aaatgaagcg aaaagag
//
LOCUS       DAOMUE010000099         6451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3238_length_6451_cov_2.663383, whole genome shotgun sequence.
ACCESSION   DAOMUE010000099 DAOMUE010000000
VERSION     DAOMUE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3238_length_6451_cov_2.663383"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..>783
                     /locus_tag="PLC71_05760"
     CDS             92..>783
                     /locus_tag="PLC71_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRM02181.1"
                     /translation="MYKIVKKQTLNQAVELMEIHAPYVARKCEPGQFIIIRVDEDGER
                     VPLTIADYDREKETVTIIYQVVGYTTELLSKKAEGDYVQDFVGPLGQPAPLHKCDRVI
                     GVAGGVGAAPLYPQLRKLAKMGVPVDVIIGGKSAEFVILKELFEEFCDNVYIATDDGS
                     LGTKGFVTTVLEDQIKAGVKYDEVIAIGPLIMMKNVVKITKEYDIPTMVSLNPIMIDG
                     TGMCGGCRVNIGG"
     assembly_gap    784..793
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <794..914
                     /locus_tag="PLC71_05765"
     CDS             <794..914
                     /locus_tag="PLC71_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRM02182.1"
                     /translation="FACVDGPDFDGFKVNFDECMQRQGMFREEEHECRIGRGM"
     gene            918..2309
                     /gene="gltA"
                     /locus_tag="PLC71_05770"
     CDS             918..2309
                     /gene="gltA"
                     /locus_tag="PLC71_05770"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HRM02183.1"
                     /translation="MANMSLKKVAMPEQDPNVRNKNFEEVALGYTKEMAMEEAARCLN
                     CKNKPCVGGCPVNVPIPAFIEKVAAGDFEGAYEVITTENALPAICGRVCPQENQCEGK
                     CVRGIKGEPVGIGRMERFVADWHMANAKETEVNIEKNGMKVAVVGCGPAGITCAGELI
                     KKGYDVTVFEALHKPGGVLSYGIPEFRLPKDLVAKEIESVQKLGVDIETNVIVGRSIT
                     IDELMEDGYKAVFVGSGAGLPRFLNIPGENHLGVYSANEFLTRVNLMKGYKFPECPTP
                     VKVGKKVAVVGAGNVAMDAARTAKRLGAEEVYIVYRRSEEEAPARLEELHHAKEEGII
                     FKFLNNPAAIKADENGWVSSMEVIKQELGEPDASGRRSPQPIEGSNYDLDVDTVIIAI
                     GQSPNPLIRHTTPGLDTQKWGGIIVNEETMETSKDGVYAGGDTVTGAATVILAMGAGK
                     KAAAAIDQKLQGK"
     gene            2324..2692
                     /locus_tag="PLC71_05775"
     CDS             2324..2692
                     /locus_tag="PLC71_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HRM02184.1"
                     /translation="MGKAIFQTEIIELGEQVDAFFEEGMFVLFGDNVPDTLKDFCHFI
                     DQKKVDGTIKKGDKLVVDQKEYLITAVGDIAQSNLETLGHLTVVFSGAKEAGLPGSIC
                     VEAKPMPKLAIGSKISIVEE"
     gene            2964..4415
                     /gene="guaB"
                     /locus_tag="PLC71_05780"
     CDS             2964..4415
                     /gene="guaB"
                     /locus_tag="PLC71_05780"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRM02185.1"
                     /translation="MGKLIGEGITFDDVLLVPAFSEVIANDVDTRTRLTNKIQLNIPL
                     MSASMDTVTEHRMAIAMARQGGIGIIHKNMSIEEQAEEVDKVKRSENGVITDPFSLSP
                     EHTIQDADDLMAKYRISGVPITEGTKLVGIITNRDLKFETDFSKKIKESMTSEGLVTA
                     KEGITLEEAKQILGKARKEKLPIVDDDFNLKGLITIKDIEKQIKYPNAAKDDQGRLLC
                     GAGVGITADVLDRVQALVNAHVDVIVVDSAHGHSANVLRVVRMVKDKFPDLQVIAGNV
                     ATGAGAKALIEAGADCVKIGIGPGSICTTRVVAGIGVPQITAIMSAYEEAKKAGIPII
                     ADGGIKYSGELTKAIAAGADVCMLGSMLAGCDESPGDFELYQGRKYKVYRGMGSLAAM
                     ENGSKDRYFQTNAKKLVPEGVEGRVAYKGTVEDTIFQMMGGLRSGMGYCGAKDIKTLQ
                     ETGEFVKISAASLKESHPHDIHITKEAPNYSVE"
     gene            4628..>6451
                     /locus_tag="PLC71_05785"
     CDS             4628..>6451
                     /locus_tag="PLC71_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRM02186.1"
                     /translation="MISKFSVKKPYTVIVAVVLVIILGIVSFTKMTTDLLPSMELPYA
                     IVMTTYQGASPETVETTVTKPVEQSMATISNIKEVNSISSENMSMVILEFEGDTDMNA
                     VSLDMREKLDMIKGYWPDEVGNPTIVKLNPDMMPVMVTAMDSDKLSNTELSELINDKI
                     APSLEAVDGVASVSSTGLVTEQINAIISEKKINKLNEKIEKALNGQFDEAQNKIDQAR
                     AQMESGKNTLDAQRTKANTQLAKAQTALTDGQLSLAQKEVQVTSSITNLKVQKQSLQT
                     SLKTLKTQIAKMEEQAKQVPDAATKMQLAAQIEELKKQETTAKSSIKALDKNLKTLNN
                     ALKQIKKGKKTINSKLTQFNVQSATATQKMNDGEIKLAQGEAQLNSSQQQLDSSKEQA
                     KEAANIKNKLTVANVKALLTAQNFEMPAGYISEGNTQYLVRVGDKVTNQKDLANMELL
                     DLGIKGIPPVKLCDVADVAIVDNSADTYCRVNGNNGVVLTVQKQNNYSTADVADKVAA
                     KMKTLTKENKGFHYVNIMDQGVYIDMVTGSVIQNLLMGAILAVFILLLFLKDVRPTIV
                     IACSIPLSVIFAVVLMYFTGITLNVISLSGLALGVGMLVDNS"
BASE COUNT         2283 a         1023 c         1528 g         1607 t
ORIGIN      
        1 tgccgacaaa aaataaataa aaaaacactt tcttattgtg tagattccat gtataataaa
       61 aagaagatat tttaagaaag aggtgtacgt ggtgtataag attgtcaaga agcagacact
      121 caatcaggca gttgagttaa tggagattca tgctccttat gtggcaagaa aatgtgaacc
      181 gggacagttc attattatcc gagtagatga agatggagaa agagttcctt taacgatcgc
      241 agattatgat cgtgagaaag agacagtaac gatcatttat caggttgtag gatatacaac
      301 agaattgtta agcaagaaag cagaaggaga ttatgtacag gatttcgtgg gaccattagg
      361 tcagccagct cctttgcaca agtgtgaccg tgtcatcggt gtagccggtg gtgtaggtgc
      421 agccccatta tatccacagc ttcgtaagtt agcgaagatg ggagtaccag ttgatgtgat
      481 cattggtggt aagagtgcag aatttgttat tcttaaggaa ttattcgaag aattctgcga
      541 taatgtatat attgcaacag atgacggaag tcttggaacc aaaggtttcg ttacaacagt
      601 attagaagac cagatcaaag ctggagtaaa atacgatgaa gttatcgcaa tcgggccact
      661 tatcatgatg aagaatgtgg ttaagatcac aaaagaatat gacatcccaa ctatggtatc
      721 tttaaatccg atcatgatcg acggaacagg aatgtgcggt ggatgccgtg tcaacatcgg
      781 tggnnnnnnn nnnattcgca tgtgttgacg gaccagattt tgatggattt aaagtaaact
      841 ttgacgaatg tatgcagcgt cagggtatgt tcagagaaga agaacatgaa tgtagaattg
      901 gaagggggat gtaaacgatg gcaaatatga gtcttaagaa ggtagctatg ccagaacagg
      961 acccaaatgt ccgtaataag aactttgaag aagttgctct tggatataca aaagagatgg
     1021 caatggaaga agcagctcgt tgcttaaact gtaagaataa accttgtgtt ggaggatgtc
     1081 cggttaacgt accaattcca gcatttatcg agaaagtagc agcaggagat tttgaaggtg
     1141 catacgaagt gatcacaaca gagaatgcat tacctgcaat ctgcggacga gtatgtccac
     1201 aggagaacca gtgtgaagga aaatgtgtcc gtggtatcaa gggagagcct gttggtattg
     1261 gacgtatgga acgtttcgtc gcagactggc atatggcaaa cgccaaagaa acagaagtta
     1321 atattgagaa gaacggaatg aaagtagcag ttgtcggatg tggacctgct ggtatcacat
     1381 gtgccggaga actgatcaag aaaggttatg atgtaacagt ctttgaagca ttacataaac
     1441 caggtggagt attaagttat ggtattccag aattccgtct gccaaaagat ctggtagcaa
     1501 aagagatcga aagcgttcag aagttaggtg tagatattga aacaaacgtt atagttggac
     1561 gttctatcac aattgatgaa ttaatggaag atggatacaa agcagtcttc gtaggaagtg
     1621 gtgcaggact tccaagattt ttaaatattc ctggagaaaa ccacttagga gtatattctg
     1681 caaatgaatt cttgactcgt gtaaacttaa tgaagggata caaattccca gagtgcccaa
     1741 ctccagtcaa agttggtaag aaagtagcag tcgtaggtgc tggtaacgta gcaatggatg
     1801 ctgccagaac agcaaaacga ttaggtgcag aagaagttta catcgtatat cgtcgtagcg
     1861 aggaagaagc tcctgctcgt ttagaagagc ttcatcatgc aaaagaagag ggaatcatct
     1921 tcaaattctt aaataaccca gctgcgatca aagcagatga aaacggatgg gtaagcagca
     1981 tggaggtcat taaacaggaa ctgggtgaac cagatgcatc tggaagacgt tctccacagc
     2041 caatcgaagg atctaactat gatcttgatg ttgatacagt catcattgcg atcggacaga
     2101 gccctaaccc actgatccgt cacacaacac caggacttga cactcagaag tggggtggaa
     2161 tcattgtcaa tgaagagaca atggagacaa gcaaagatgg tgtgtacgca ggtggagata
     2221 cagtaactgg agcagcaaca gttatccttg ccatgggagc aggaaagaaa gcagcagcag
     2281 caatcgatca gaaattacag ggtaaataaa aggaggattc caaatgggaa aagcaatatt
     2341 tcagactgag attatcgaac tgggagaaca ggtagatgct ttttttgagg aaggtatgtt
     2401 tgtattattc ggagacaatg taccagatac attaaaagat ttctgtcatt tcatcgatca
     2461 gaagaaggta gacggaacaa tcaaaaaagg agataaactg gttgtggatc agaaagaata
     2521 tctcatcact gccgtaggag atattgccca gtctaatttg gagacactgg gacatttaac
     2581 cgttgtcttc agtggagcga aagaagcagg tcttcctgga agtatttgtg ttgaggcaaa
     2641 gccaatgcca aaacttgcga ttggaagtaa aatttctatc gtagaagagt agagattaca
     2701 agagatatca agcggattga tatcaagatc atagaaaagg agttattgtg aaagcagtaa
     2761 ttccttttct tgtacttaaa aagatgtaaa gtgtggattg ctatatttag aatattcaga
     2821 taattccaca catgtacaga aaataatcca tacgttttaa aaaaagtata aaataatctg
     2881 ttgacatcaa agaaactatt tgttaacata tctaacaata caaatcagat attaaaatac
     2941 aagaaagtga ggaaaacaag aaaatgggta agttaattgg tgaaggaatt acatttgatg
     3001 atgttttatt agtaccagca ttttcagaag tgattgcgaa tgatgtggat acacgtacac
     3061 gattgacaaa caagattcag ttaaatattc cattgatgag tgccagcatg gatactgtta
     3121 cagaacaccg catggcaatc gcaatggcaa gacagggtgg aattggtatt atacataaga
     3181 acatgtcaat tgaagaacag gcagaagaag tggataaggt aaaacgttct gaaaacgggg
     3241 ttatcacaga tccattttca ttatcaccag aacataccat tcaggatgct gacgacttaa
     3301 tggcgaagta tcgtatttcc ggtgtaccaa ttacagaagg tacaaagctt gttggtatta
     3361 tcacaaaccg tgacttaaag ttcgagacag atttctctaa gaagatcaaa gaatctatga
     3421 cttctgaagg actagtaaca gctaaagaag gaattacatt agaagaagca aaacagattt
     3481 taggaaaagc aagaaaagaa aaattaccaa tcgttgatga tgactttaat ttaaaaggat
     3541 tgatcacgat caaagatatt gagaaacaga tcaaatatcc aaatgcagcg aaagatgatc
     3601 agggaagatt attatgtgga gctggtgttg gtatcacagc agacgtttta gatcgtgtgc
     3661 aggcattagt gaatgctcat gttgacgtta tcgttgttga ctcagcacat ggacattctg
     3721 caaacgtatt aagagtcgta agaatggtca aagacaaatt cccagatctt caggtgatcg
     3781 caggtaacgt tgcaacagga gcaggagcaa aagcactgat cgaagcagga gctgactgtg
     3841 tgaagatcgg tatcggacct ggatctatct gtacaactcg tgtcgtagct ggtatcggtg
     3901 taccacagat cacagcgatc atgagtgcat atgaagaagc taagaaagca ggaattccaa
     3961 ttattgccga tggtggtatc aaatattcag gagaacttac aaaagcgatc gcagctggag
     4021 ctgatgtatg tatgttagga agtatgcttg caggatgtga cgaaagccct ggagactttg
     4081 aactatacca gggaagaaaa tacaaagtat atcgtggaat gggatcctta gcagctatgg
     4141 agaatggtag taaagaccgt tacttccaga caaacgctaa gaagttagtt ccagaaggtg
     4201 tcgaaggacg tgttgcatat aaaggaactg ttgaagacac aatcttccag atgatgggtg
     4261 gtcttcgttc tggaatggga tattgtggag caaaagatat caagacatta caggagacag
     4321 gagaatttgt taagatttct gctgcatcct taaaagaaag tcatccacat gacatccata
     4381 tcacaaaaga agcaccaaac tacagtgtag aataagagat caagaatata cagaagccag
     4441 agtacataag aagatgtatt ctggtttctt tttgttgtgt gaaaaaagtt cataatccga
     4501 tacattaatt gacaattatc aataaaagtg attgacttct ttaagaaaca ggaatatcat
     4561 taaattactt tggctatcaa aagagacaaa caaggaataa gaaaagaaat gtgagaaagg
     4621 taagaaaatg atctcaaaat tcagcgtaaa gaaaccgtat acagtcattg tagcggttgt
     4681 actagttatc attttaggaa tcgtgtcatt tacaaagatg acaacagact tgcttccaag
     4741 tatggaactg ccatatgcaa tcgttatgac gacatatcag ggagcatctc cggagactgt
     4801 ggaaacgaca gttacaaaac cagtggaaca atcaatggca acgatttcaa atatcaaaga
     4861 agttaattca atttccagtg agaatatgtc aatggtcatt ttggaatttg aaggtgacac
     4921 ggatatgaat gcagtatctc ttgatatgag agagaagctt gacatgatca aaggctactg
     4981 gccagacgaa gtaggcaatc caacgatcgt gaaattaaat ccagatatga tgccggttat
     5041 ggtaacagct atggattctg ataagttaag taatacggaa ttatcagagt tgatcaatga
     5101 taagattgca ccatctttag aagctgttga cggagtcgca tctgtaagta gtacaggact
     5161 tgtaacagaa cagatcaatg cgatcatcag cgagaagaag atcaataaat taaatgagaa
     5221 gatcgagaag gcattgaatg gtcagtttga tgaagctcaa aataagattg atcaggcaag
     5281 agcccagatg gaatctggaa agaacacatt agatgcacag agaacaaagg ctaatactca
     5341 gcttgcgaaa gctcagactg cacttacgga tggacagtta agtcttgctc agaaagaggt
     5401 gcaggtaacg agttcaatca cgaatttgaa ggtgcagaaa caatcgttgc agacatccct
     5461 caagacatta aaaacacaga tcgccaagat ggaagaacag gcaaaacaag ttccggatgc
     5521 agcaacaaag atgcagcttg cggctcagat cgaagaactg aaaaagcagg agacaacagc
     5581 aaaaagttcg atcaaagcat tggataagaa tttaaaaaca ttaaacaatg cattaaaaca
     5641 gatcaaaaaa ggaaagaaaa cgatcaattc taaactgaca cagtttaatg tacaatcagc
     5701 aacggcaacc cagaagatga atgatgggga gatcaaactg gcacagggag aagcacagtt
     5761 gaattccagc cagcagcaac ttgattccag caaagagcag gcaaaagaag ctgcaaatat
     5821 taagaataag ctgacagttg cgaatgtcaa agcactgtta acagcacaga actttgagat
     5881 gccggcagga tatatcagtg aagggaatac acagtatctg gtacgtgttg gagataaggt
     5941 gacgaatcaa aaagatcttg ccaatatgga attattagat cttggaatca aaggaatccc
     6001 accggtcaaa ctttgtgatg tagcagacgt agcgattgta gataactcag cagatacata
     6061 ttgtcgtgtg aatggaaata acggtgttgt attaacagtc cagaaacaga ataattattc
     6121 tacagctgat gtagcagaca aagttgcagc gaagatgaag acacttacca aagaaaataa
     6181 aggattccat tatgttaata ttatggatca gggtgtatac attgatatgg tgacaggttc
     6241 tgttattcag aatctgttaa tgggagcaat ccttgcagtc tttatcctat tattattttt
     6301 gaaagacgtc agaccgacga tcgttattgc atgctccatt ccgttaagtg tcatttttgc
     6361 ggtggtgttg atgtacttta caggaattac gctaaatgta atctccttgt cgggactcgc
     6421 acttggagtg ggaatgcttg tggacaactc g
//
LOCUS       DAOMUE010000100         6422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3259_length_6422_cov_2.101775, whole genome shotgun sequence.
ACCESSION   DAOMUE010000100 DAOMUE010000000
VERSION     DAOMUE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3259_length_6422_cov_2.101775"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLC71_05790"
     CDS             complement(<1..769)
                     /locus_tag="PLC71_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HRM02187.1"
                     /translation="MALFESYERREKQILAVLKEYGINSIEEAGEITKAAGLDVYAQV
                     EGIQPICFENAKWAYTVGAAIAIKKGCKRAADAAAAIGEGLQAFCIPGSVADQRKVGL
                     GHGNLGKMLLEEETECFAFLAGHESFAAAEGAIGIAEKANKVRQKPLRVILNGLGKDA
                     AQIISRINGFTYVETEMDYYTGEVKEVFRKSYSEGLRAKVNCYGANDVTEGVAIMHKE
                     GVDVSITGNSTNPTRFQHPVAGTYKKECIEQGKKYFSV"
     gene            complement(783..1475)
                     /locus_tag="PLC71_05795"
     CDS             complement(783..1475)
                     /locus_tag="PLC71_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02188.1"
                     /translation="MIYSHEVEQMCTVAQGVNHGAAPIPEEAKWVKAKDVTDISGLTH
                     GIGWCAPQQGGCKLTLNVKEGIIQEALVETIGCSGMTHSAAMASEILPGRTILEALNT
                     DLVCDAINTAMRELFLQIVYGRTQSAFSEEGLPIGAGLEDLGKGLRSQVGTMYGTLKK
                     GPRYLEMAEGYVTGIALDADDEIIGYQFVNFGRMMDFIKAGDDAQTALDKAKGQYGRV
                     DDAVKIIDPRKE"
     gene            1764..3047
                     /locus_tag="PLC71_05800"
                     /pseudo
     CDS             1764..3047
                     /locus_tag="PLC71_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392878.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            complement(3203..3931)
                     /locus_tag="PLC71_05805"
     CDS             complement(3203..3931)
                     /locus_tag="PLC71_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM02189.1"
                     /translation="MSGHSKFANIKHKKEKNDAKKGKIFTVIGREIAVAVKEGGPDPD
                     NNSRLRDVIAKAKANNMPNDTIERGIKKAAGDADSVNYEQITYEGYGPSGVAIIVDTL
                     TDNKNRTASNVRNAFTKGNGNVGTPGCVSYMFEKKGQIIISKEECEMDADDLMMMALD
                     AGAEDFNEEDDSFEVLTTPEDFSAVREALEKEEIPMADASVTMIPGTMVELTSEEDIK
                     SIQKTLDLLDEDDDVQEVYTNWDE"
     gene            complement(3915..4865)
                     /locus_tag="PLC71_05810"
     CDS             complement(3915..4865)
                     /locus_tag="PLC71_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRM02190.1"
                     /translation="MEGFTPSVLMMIILAVIIGFAVGCFFMKQREKIKEAKKKLHTSE
                     QEHEEDEDNEYEEEIMNIVNEGHEQGAIMEGEARMITNIFEFGDKDVTDVMTSRKKID
                     AIDVNMSVEKALNYMLDEPYSRYPLYEDDIDNIVGVLYLKDVIDAYLNQKEATLSDIA
                     REPFFVHQTMNLSVLFQEMQTKKIHMAIVIDEYSQTEGIVSMEDMLEVIVGNILDEYD
                     VEDRNITKIGWADIYLVRGSTRLEELEDTLGIEFDVEDIETLNGFLIDQLGHLPEDNE
                     EIEIIYQGYSFKSVDIHDNMIRQVKVEKRKRKEEEDVRTQ"
     gene            complement(4963..5310)
                     /locus_tag="PLC71_05815"
     CDS             complement(4963..5310)
                     /locus_tag="PLC71_05815"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRM02191.1"
                     /translation="MVIKRGDIFYADLRPVVGSEQGGIRPVIVLQNDAGNKYSPTIIC
                     AAITSKMNKAKLPTHVPIDCHRCELEKDSVILLEQIRTIDKSRLREKVCHLNCTFLKQ
                     VDHALKISLSLDT"
     gene            complement(5382..6419)
                     /gene="alr"
                     /locus_tag="PLC71_05820"
     CDS             complement(5382..6419)
                     /gene="alr"
                     /locus_tag="PLC71_05820"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRM02192.1"
                     /translation="MATAKAIAKELNKIGVDAFAVAILEEGITLRNNGIRQPILILGY
                     TSEYQYSSLIQYEIEPTVFCYEMAESLSKIAQALGKDAKIHIKLDTGMNRIGFKPTKE
                     SVDIIEKIAKLPNIKIQGIFTHFACADEADKTETYEQERKYDQFITWLEERDIYIPIK
                     HVSNSASIIDLDGFRKDMVRSGIITYGLYPSEEVSKDVLDLRPAMELKTHIVYIKEVE
                     AGEGISYNHTYVTDKKTKIATIPVGYADGYPRSLSSKGKVLIRGQYAPIIGRICMDQF
                     MVDVTDIEGVSVMDEVTLVGHDGNKMLTVEEVANEAGSFNYEFVCGIGKRVPRVYIRN
                     GEMKEAEYFEK"
BASE COUNT         1753 a         1359 c         1023 g         2287 t
ORIGIN      
        1 ctacagagaa gtatttttta ccctgttcga tacattcttt tttgtatgta cctgcaactg
       61 gatgctggaa acgagttggg tttgtagagt tacctgtgat agatacgtct acaccttctt
      121 tgtgcatgat tgcaacacct tctgttacat cgttagcacc gtagcagtta accttagctc
      181 ttaatccttc agagtaagat tttctgaata cttctttaac ttcacctgtg taataatcca
      241 tttcagtttc aacatatgta aatccgttaa ttctggaaat gatctgtgca gcatcttttc
      301 ctaatccgtt taagataact cttaaaggtt tctgacgaac tttgtttgct ttttcagcga
      361 ttccgatagc tccttcagca gctgcgaaag attcgtgacc tgctaaaaat gcgaaacatt
      421 ctgtttcttc ttcaagaagc atctttccta agttaccatg tccaagacct actttacgct
      481 gatcagctac agatccagga atacagaatg cctgaagacc ttctccgata gcagcagcag
      541 cgtctgcagc tcttttgcaa ccttttttga ttgcgatagc agcacctact gtgtaagccc
      601 attttgcatt ttcgaaacag attggctgaa ttccttcaac ctgagcataa acatctaatc
      661 cagcagcttt tgtgatttca ccagcttctt cgatagaatt gattccatac tcttttaaaa
      721 cagcaagaat ctgtttttct cttctttcat atgattcaaa taaagccatt attcgctacc
      781 tcctattcct ttcttgggtc aataatctta acagcgtcat caacgcgtcc gtactgacct
      841 ttggccttgt caagtgctgt ctgagcatcg tctccagctt tgatgaagtc catcattcta
      901 ccaaagttaa caaactggta tccaatgatt tcatcatcag cgtcaagagc gatacctgtt
      961 acatagccct ctgccatttc caggtaacga ggaccttttt taagtgttcc gtacattgta
     1021 ccaacctgag atcttaatcc tttacctaag tcttcaagac cagcaccgat tggaagtcct
     1081 tcttcagaga atgcactctg agttcttccg tatacgatct gtaagaataa ttctctcatt
     1141 gctgtattga tagcatcaca aactaagtct gtattcaatg cttctaaaat tgttcttcct
     1201 ggtaagattt cagatgccat agcagctgaa tgtgtcattc ctgaacatcc gattgtttct
     1261 actaatgctt cctggatgat accttcttta acgttaagag ttaatttaca tcctccctgc
     1321 tgaggtgcac accaaccaat accatgtgtt aatcctgaga tgtctgttac atctttcgct
     1381 tttacccatt ttgcttcttc aggaattggg gcagctccat ggtttacacc ctgagctact
     1441 gtacacattt gttctacttc atgtgaataa atcatgttga aactcctttc agtattaaaa
     1501 ataacatctt tattttagca catcgttaaa aataaaacaa caaattatct taagaacttc
     1561 gttaagtttt tcaatgtttt tgttggtttg tgtgataaaa ataatttttt tgtcaagttg
     1621 tttttctttt atataaaaaa tctcgaattt tctttttttt tagtcaattc aataaattat
     1681 ctgaattcta ttccccttgt tttctttagg aattacgggt ataatttaat acatattttt
     1741 tataacagaa aggaattatg attatgagtc attatgattt agaaacaaaa tgtcttcatt
     1801 ctggttacac tcctaagaaa ggagaaccat gtgcacttcc tatttatcag agtactactt
     1861 tcaaatatga tacaaccgat gaaatgggac agttatttga tctgaaagca gaaggatatt
     1921 tctatacacg tcttcagaat cctacgaacg atgctgtagc tgccaaaatt gctgatcttg
     1981 aaggaggtgt tgcaggactt cttacttctt ctggacaggc tgcaaacttc tatgcgatct
     2041 ttaatatctg tgaagctgga gatcatgttg ttgcagcttc tacgatctat ggtggaacat
     2101 ttaaccttct gggtgttaca ttaaagaaac ttggaattga ctgtacattc gttgatactg
     2161 atgcaagtgc tgaagaaatc gctgctgcat tcaaaccgaa taccaaagtt ttatttgcag
     2221 agacgattgc aaaccctgca cttgtcattc ttgatattga gaaatttgca aaggttgccc
     2281 atgaacatga agttccattg atcgttgata atacttttgc aacaccgatc aactgtcgtc
     2341 cttttgaatg gggtgctgac atcgtcacac attcaacaac aaaatatatg gatggacacg
     2401 ccgttcaagt tggcggtgcg atcattgaca gcggaaactt tgactgggac gcttatggac
     2461 acaaatatca tggtcttact gaacctgatg aatcttacca tggtgtcgtt tatacaaaac
     2521 aatttggaaa gaaagcttat atcacaaaag ctacaacaca gctgatgcgt gacctcggtt
     2581 ccattccatc ccctgaaaac tgtttcttat taaatcttgg ataagaaact ctgcctttgc
     2641 gtgtagaacg tcattgtgaa aatgctcaga aagtcgctga attcttagat gctcatgaaa
     2701 aagtatctca tgttatttat gccggacttc ctaatgataa atatcacgaa ttagcacaaa
     2761 aatatatgga taatggaaga acctgtggtg ttatttcctt tgaactgact ggtggacgtg
     2821 atgctgcagt acgctttatg gatagcttaa aattagcgaa catcgcaact catgttgctg
     2881 catccaaaac gatggtactt caccctgcaa gtcatacaca tcgccagatg aatgatgaac
     2941 agttaagaga agctggtgtt tctcctggaa tgatccgttt atccgttgga attgaaagtg
     3001 caaaagatat tatcaatgat ctgaaagaag cacttgagaa tgcatagttt tataggtgtt
     3061 cgaagattac ttagtaacag taataattca tattataaat ttcaaatatc atagcaaaaa
     3121 agaaccttaa ctcccagcat ttattatata tgcattgagt taaggttctt attattttat
     3181 attctataaa atctataaca gattattcat cccagtttgt ataaacttct tgcacatcgt
     3241 catcctcatc aagaagatct aatgttttct gaatactctt aatatcttct tcacttgtaa
     3301 gttcgaccat agtaccaggg atcattgtga cactggcatc tgccatagga atttcttcct
     3361 tctctaaagc ttctctgact gcactgaagt cttctggtgt tgtcaatact tcaaagctgt
     3421 cgtcttcttc gttgaagtct tctgctccgg catctaatgc catcatcata agatcgtctg
     3481 catccatttc acattcttct ttagagatga tgatctgtcc tttcttctcg aacatataag
     3541 atacacatcc tggtgttcct acgttaccat ttccttttgt gaaagcattt cttacattag
     3601 atgctgtacg gtttttgtta tctgttaatg tatctacgat gatcgcaact ccgcttggtc
     3661 catatccttc atatgtaatc tgctcataat taacagaatc tgcatctccg gcagcttttt
     3721 tgataccacg ttcgatcgtg tcatttggca tattatttgc ttttgctttt gcaatgacat
     3781 cacgtaatct gctgttgtta tctggatctg gtccaccttc tttaactgct actgcaattt
     3841 ctcttccaat gactgtgaaa atctttcctt ttttcgcatc atttttttct tttttgtgtt
     3901 tgatgttcgc aaatttactg tgtcctgaca tcttcttctt ccttcctttt ccttttttct
     3961 acctttacct gtctaatcat gttgtcatga atatcaacag atttaaagga atatccttgg
     4021 tagataattt ctatttcttc attgtcttct ggtaaatgtc ccagctgatc aattaaaaat
     4081 ccattgagtg tctcaatatc ttcaacgtca aattcgattc ctaatgtatc ctcaagttct
     4141 tccagtctcg tacttcctcg tacaagataa atatctgccc atccaatctt ggtgatatta
     4201 cggtcttcga catcatattc gtctaaaata ttccctacta tgacctctag catatcttcc
     4261 atagatacaa taccttctgt ctggctgtat tcatcaataa cgatcgccat atgaatcttc
     4321 ttggtctgca tctcctgaaa aagcaccgat aaattcatcg tctgatgaac aaaaaacggc
     4381 tctcttgcaa tatcagaaag tgttgcttct ttctgattaa gatatgcatc gatcacatct
     4441 tttaaataaa gcactcctac aatgttatca atgtcatctt catataatgg gtatcttgaa
     4501 tatggctcat ctaacatata atttaatgct ttctctacgg acatgtttac atcaatcgca
     4561 tcaatcttct ttcttgatgt cataacatct gtaacatctt tatctccaaa ttcgaaaata
     4621 ttcgtgatca tgcgggcttc cccttccatg atcgcacctt gctcatgtcc ttcgttaaca
     4681 atgttcatga tctcttcttc atattcattg tcttcatcct cttcatgttc ttgttctgat
     4741 gtgtgcagtt ttttctttgc ttctttgatc ttctctcttt gtttcatgaa aaaacaccca
     4801 actgcaaatc cgatgataac tgctaaaatg atcatcataa gcaccgaggg agtaaatcct
     4861 tccatttctt tttttttctc cttttatctt ctagaaatta agactataca aacttaaata
     4921 tatcatttct aagaaaaaag gtcaatatat ccaagattcc tgttatgtat caagacttaa
     4981 actgatcttt agtgcatgat ccacttgttt taggaatgta caatttaaat ggcatacctt
     5041 ttctcttaaa cgacttttat cgattgtccg gatctgttct aacaaaatca cagaatcttt
     5101 ttctaattca catctgtggc agtcgatcgg tacatgagtc ggcaactttg ccttgttcat
     5161 cttgcttgtg atcgctgcac agatgatcgt tggactatat ttatttcccg catcattttg
     5221 caatacgatc acaggacgga taccgccttg ttctgaacca acaaccggtc ttaaatctgc
     5281 ataaaatata tcgcctcgct taattaccat aagcataaac actctccatt tctttttaaa
     5341 tattgtatga agagtattta cgattctttc aaaagtattc cttatttttc aaaatattct
     5401 gcttctttca tctctccgtt tcgaatgtat actcgtggaa cacgtttccc aatcccacag
     5461 acaaattcat aattaaaaga tcctgcttca tttgcaactt cttctacggt caacatttta
     5521 ttgccatcgt gtccgactaa agttacttca tccatcacag atacaccttc aatatctgtc
     5581 acgtcaacca taaactgatc catacagatt cttccaatga tcggtgcata ctgcccacgg
     5641 atcaatactt ttcctttgga agagagtgat cttgggtatc cgtctgcata tcctactggg
     5701 atagttgcga tctttgtctt cttatcagtt acatatgtgt gattgtaact gattccttct
     5761 cctgcttcca cttctttgat atatacaatg tgtgtcttta attccattgc tggtcttaaa
     5821 tccaatacat cttttgaaac ttcttctgat ggatataatc cataagtaat gattcctgaa
     5881 cgtaccatat cttttctaaa tccatccaga tcaatgatcg atgcactgtt tgatacgtgt
     5941 ttgattggaa tatagatatc tctttcctca agccacgtaa taaactgatc atattttcgt
     6001 tcctgctcat aagtttctgt tttatccgcc tcgtctgcac atgcgaaatg tgtaaagatt
     6061 ccttgaatct tgatatttgg taatttggcg atcttctcta tgatatctac agattctttg
     6121 gttggtttaa atccgatacg gttcattcct gtatccagtt tgatatgaat cttcgcatct
     6181 ttgcctagtg cctgcgcgat ctttgataag gactctgcca tctcatagca gaatacggtt
     6241 ggctcgatct catactgaat caatgagctg tactgatatt ctgatgtgta tcctaaaatc
     6301 aaaattggct gtctgattcc attgtttcga agtgtgatcc cttcttctag gattgcaacg
     6361 gcaaatgcat caactccgat cttatttaat tcttttgcaa ttgctttagc tgttgctata
     6421 tg
//
LOCUS       DAOMUE010000101         6415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3265_length_6415_cov_2.586478, whole genome shotgun sequence.
ACCESSION   DAOMUE010000101 DAOMUE010000000
VERSION     DAOMUE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3265_length_6415_cov_2.586478"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="PLC71_05825"
     CDS             <1..1422
                     /locus_tag="PLC71_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5717 family protein"
                     /protein_id="HRM02193.1"
                     /translation="IFDEHASFVFFDSEENRYIGSVAYQLKPIWTKEQIQAYCEAADG
                     TNEHFVLYKGAAFERKQQLEDEDFPVVLSALKSRALDEDYEQQLFEKVLDYYWEHEMS
                     EELKRALSYVEWKNLKDENRIHMIRYFIAGELYNEALKGIEQYGYQFLDKQQLCTICL
                     YALTTLSTRRSELLVEMCEYAFSRGEENSEIVAYLQRYFHGTKEEMLAIFEAGNKGGM
                     YDRIFVESVLKACIADGVDGTEFKVFSTYIQQVQTDTALIDAMLVEYVNFAYEQDQIL
                     PEEFYELIGKKIDIGKMDWMYQQLYLDYYKDKKENLSDRCKGRIEKIKDHQVVQTDHV
                     LPVLFQYLDVIKLPKYLCARTFIEFRAKSGQTVIFHFMDGTSAAWKEEVMKELLPGYY
                     VFSASIFDHELNDHYVTIEGEERRYRDQVVVTDHLKYCKGSRFYELNELIRHQQDGKL
                     QMMMEKYAAKTMMVQFIKPMTEE"
     gene            1426..2460
                     /locus_tag="PLC71_05830"
     CDS             1426..2460
                     /locus_tag="PLC71_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HRM02194.1"
                     /translation="MQTAIHFEGDLAYICVSDQGEIKEEEPVTYGRSRVEFVISTAQA
                     QKEGTIGVVLDEAAPQIVQQAEEQCIRAGITKERVRFFTKEEAALGVVGFRGDNLLRG
                     NSVIFDYTKKRFICYEIRKTKDRVLVDSRDYTEQIKDAVANEEKDIAFLQIIKQALVR
                     GVTATVYLCGEGFEGSWFKQSTKALCLGRRVFMSKHLFACGAVYLCENDKHVSRDEVV
                     FAQNVTISQIGLVVHHHGRDMFCPLIMDGKPWKESRGEIEIFVSGVNGLIFEVRNRSG
                     IKKASICMSLDGMKKDPNLVYKLRIQGEYIKADQCKIKITDLGFGQIRQASYQTWQQV
                     IQLERGDYHE"
     gene            2453..3253
                     /locus_tag="PLC71_05835"
     CDS             2453..3253
                     /locus_tag="PLC71_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02195.1"
                     /translation="MSKIVLCETKPAKVPYKIAKIGRSFQSYEEFCWLLEHYLIFFLT
                     EGFEYPLKNYLKEQLDIVIKSDDAVQQVKEVINYYNYFTSDEKIQFQQKLRTTYLYSA
                     AKKQKLLADMYLKHQLYVRALIAYQKLETVSGKLNAAEQIQVLYHMGLCQSRMFCFEE
                     AKESFAMALRIKEDKQIQEAYFTAAYLAGDEEAFLEAGRKISFDEDQCKMIYQNIKER
                     EKEVFQKEEFDKLGKIDYHRKKADENITRRLIKTTLGKWKDEYKAQTI"
     gene            3228..>4080
                     /locus_tag="PLC71_05840"
     CDS             3228..>4080
                     /locus_tag="PLC71_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02196.1"
                     /translation="MNTKRRLSDDLSNDSEILSEKRFVKLYKEELESIEKQIHDLKKL
                     DQKDFDVKPEVEDKARLYYRTFAREFYDVCEGRVSDEEFFDLWEREEELKAGLNSINS
                     LEGEQKQLEKELVHANEDETMMNGKIKAAQEKRRNKKALFISFLCMAAAVCGVSAGYL
                     YHFEMNAKDYLWQLSAGAVIILLLLVILFQSQRKAGDQLKLLEMMVTHKSHTGKRLED
                     DKREIGNDLKFYYEKFEKVFSYISPEQWKLFDFCGKVSARLRFSDAIIEASADLEQLL
                     DKNQWKTS"
     assembly_gap    4081..4090
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4091..4277
                     /locus_tag="PLC71_05845"
     CDS             <4091..4277
                     /locus_tag="PLC71_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02197.1"
                     /translation="QWDNPGIWMYHPKVLYDLIKRKDYLNTLNDRKDICNQELIRHEQ
                     ILEGIGEQADSETIEGV"
     gene            4280..>5146
                     /locus_tag="PLC71_05850"
     CDS             4280..>5146
                     /locus_tag="PLC71_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRM02198.1"
                     /translation="MKKELETTYDPGAIEERLYQKWLDNKYFHAEVDETKQPYTIVIP
                     PPNITGKLHMGHALDETLQDILIRFKKMQGYNALWIPGTDHASIATEVKIINALKEEG
                     IEKEDLGREKFLERAWDWKEEYGGTIIEQMKKLGSSCDWDRERFTMDEGCSEAVKEVF
                     IKLYEKGYIYRGSRIINWCPVCQTSISDAEVEYEDQAGHFWHIKYPVVGSEGEYVEIA
                     TTRPETLLGDSAVAVHPDDERYTHLVGKMLELPLCNRQIPVVADEYVDREFGTGAVKI
                     TPAHDPNDFAMGE"
     assembly_gap    5147..5156
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5157..>6415
                     /gene="valS"
                     /locus_tag="PLC71_05855"
     CDS             <5157..>6415
                     /gene="valS"
                     /locus_tag="PLC71_05855"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRM02199.1"
                     /translation="PEINILNDDATINKNGGKYEGMDRYEARKAIVADLEEQGYLVKI
                     KEHEHNVGTHDRCGTTVEPMIKPQWFVKMEELAKPAIEAVKNGDLKFVPERYTKTYLH
                     WLENIRDWCISRQLWWGHRIPAYYCDECGEVVVARETPEVCPKCGCKHFTQDEDTLDT
                     WFSSALWPFSTLGWPEKTKELDYFYPTNTLVTGYDIIFFWVVRMVFSGYEQTGKSPFD
                     TVLIHGLVRDEQGRKMSKSLGNGINPLDVIAQYGADALRFTLITGNAPGNDMRYTEKR
                     VIASRNFANKIWNASRFIMMNLEQADFSDVTLEDLTSADKWILSKLNTLAKDVTDNME
                     KYELGIAVQKLNDFLWEEFCDWYIEMVKPRLYNDEDQTKAAALWTLNTVLTEALKLLH
                     PYMPFITEEIYCNLTDEESIMLAKWPE"
BASE COUNT         2231 a          940 c         1542 g         1682 t
ORIGIN      
        1 atttttgacg aacatgcatc atttgtgttt tttgacagtg aggaaaacag atacattgga
       61 tcagtggctt atcagttaaa accaatctgg acgaaggagc agatccaggc atattgtgag
      121 gcagcagacg gaacgaatga gcattttgtc ctatataaag gtgcagcttt tgaaagaaaa
      181 caacaattag aagacgaaga cttcccagtt gtgttatctg cattaaagag tagagcactg
      241 gatgaagact atgaacagca gttatttgaa aaagtgttgg attattactg ggaacatgag
      301 atgtctgaag aattgaaacg tgcactttct tatgtggaat ggaagaactt aaaagacgaa
      361 aacagaattc atatgatccg ctatttcata gcaggggaat tgtataatga ggctttgaaa
      421 ggaatcgaac aatacggata tcagtttttg gataagcagc aattgtgtac gatctgctta
      481 tatgcactga caacattaag taccagacgc tcagaactgt tggttgagat gtgtgaatat
      541 gcattctcaa ggggagagga aaacagtgag atcgttgcat atctgcaaag atatttccac
      601 ggaaccaagg aagagatgct ggcaatattt gaagcaggaa acaaaggtgg tatgtatgac
      661 cgcatttttg tggaaagtgt gttaaaagcc tgtatcgcag atggtgtgga tggaacagaa
      721 tttaaggtat tttctactta tattcagcag gtacagacag atacggcatt gatcgatgcg
      781 atgttagtag aatatgtgaa ttttgcatat gaacaagatc agatacttcc agaggaattt
      841 tatgaactga tcggaaagaa gatcgatatc ggaaagatgg actggatgta tcagcagctc
      901 tatcttgatt attataaaga taaaaaagag aatttaagtg acagatgtaa aggacgcatc
      961 gagaagatca aagatcatca ggtggttcag acggatcatg ttcttccagt gttgttccaa
     1021 tatctggatg tgataaaact tccaaagtat ctttgtgcca gaacatttat agaatttcgt
     1081 gcaaagagtg ggcagacagt gatattccat tttatggatg ggacaagtgc agcatggaaa
     1141 gaggaagtga tgaaagagct gcttcctgga tattatgtat tctcagcttc tatatttgat
     1201 catgaattaa atgaccatta tgtaaccata gaaggagaag aaagaagata tcgtgaccag
     1261 gtggtcgtga cagatcattt gaagtactgc aagggaagcc gcttttatga actaaatgaa
     1321 ctgatccggc atcagcagga tggaaaactc cagatgatga tggagaaata tgcagcaaag
     1381 acaatgatgg tgcaatttat aaaaccaatg acagaggaat aagatatgca gacggcaata
     1441 cattttgaag gagatctggc atatatttgt gtgtcagatc aaggagagat taaggaagaa
     1501 gaacctgtca cttatggcag atccagagtg gaatttgtca tttcgacagc acaggcacaa
     1561 aaggaaggaa cgatcggagt tgttcttgat gaagcagcgc cgcagatcgt acaacaagca
     1621 gaagaacagt gtatccgtgc aggaatcaca aaagaaaggg taagattttt taccaaagaa
     1681 gaagcagcgt tgggagttgt tggattccgt ggagataatc tgttgcgtgg aaattcagtg
     1741 atttttgatt atactaagaa acgatttatc tgctatgaga tccgcaagac aaaagatcgt
     1801 gttttagttg acagcaggga ttatacggaa cagatcaagg atgcggtggc gaatgaagag
     1861 aaagatatcg cattcttaca gatcatcaaa caggcactgg taagaggtgt gacggctaca
     1921 gtttatcttt gtggagaggg ctttgaagga agctggttta aacagtctac gaaagcattg
     1981 tgccttggaa gaagagtatt tatgagcaaa catttgtttg catgtggagc agtgtattta
     2041 tgtgaaaatg ataaacatgt ttccagagat gaggttgtgt ttgcacaaaa tgttacgatc
     2101 agtcagatcg ggctggtcgt gcatcaccat ggaagagata tgttctgtcc tttgatcatg
     2161 gatggaaagc catggaaaga aagccgtgga gagattgaaa tttttgtatc aggagtgaat
     2221 ggattgatct ttgaagtcag aaatcgttct ggaatcaaga aagcttcgat ctgtatgtca
     2281 ttagacggaa tgaagaaaga tccaaatctt gtatataaat taagaattca gggagaatac
     2341 atcaaagcag atcagtgtaa gatcaagatc acagatcttg ggtttggaca gattcgccag
     2401 gcaagctatc agacgtggca gcaggtcata cagctggaaa gaggtgatta tcatgagtaa
     2461 gatcgttttg tgtgagacaa aaccagcgaa agttccatat aagatcgcaa agattgggag
     2521 aagctttcaa agttatgagg aattttgctg gctgttagaa cattacttga tatttttctt
     2581 gacagaaggt tttgaatatc cattaaaaaa ttatttaaaa gagcagctgg atattgtgat
     2641 caagagtgat gacgcggtac agcaagtcaa ggaagtgata aattactata attactttac
     2701 ttcagatgag aaaatccagt ttcagcaaaa attacgcaca acttatcttt attctgctgc
     2761 aaagaagcaa aaacttctgg cagatatgta tttaaagcat caattgtatg taagagcatt
     2821 gatcgcttac cagaaacttg aaacagtttc agggaaatta aatgctgcgg aacagattca
     2881 ggtactatat catatgggat tatgccaaag ccggatgttt tgttttgaag aggcaaaaga
     2941 atcttttgca atggcattaa ggatcaaaga agataaacag atacaggaag cgtattttac
     3001 agcagcatat ctggcaggag atgaggaagc atttttggaa gcaggaagaa agatttcttt
     3061 tgatgaagat caatgtaaga tgatctatca gaacataaaa gaaagagaaa aagaagtttt
     3121 tcaaaaagaa gagtttgata aacttggtaa aatagattat catagaaaga aggcagacga
     3181 aaatataacc cgccgcctga ttaagacaac tttagggaag tggaaagatg aatacaaagc
     3241 gcagactatc tgatgacctg tcgaatgata gtgagatctt aagtgaaaaa cgatttgtaa
     3301 aactttataa agaagagtta gaaagcattg agaaacagat ccatgatctt aagaaacttg
     3361 atcagaaaga ttttgatgtg aaaccagagg tagaggacaa agcaagactt tattatcgga
     3421 catttgcaag agaattttat gatgtttgtg aaggaagagt cagtgatgaa gagttttttg
     3481 atctttggga acgcgaagaa gaattaaagg caggactcaa ttcgatcaat tctctggaag
     3541 gagaacaaaa acagttagag aaggagcttg tgcatgcgaa tgaagatgag accatgatga
     3601 atgggaagat caaggcagca caggagaaga gaagaaataa aaaggcactg tttatttcat
     3661 ttctttgcat ggcagcagcg gtatgcggag tgagtgctgg atatttatat cattttgaga
     3721 tgaatgccaa agattatctg tggcagttga gtgcaggagc agtgatcata ttattgcttc
     3781 ttgtgatttt atttcagtca cagaggaaag ctggtgatca gctgaaatta ttagagatga
     3841 tggtcacaca caagagtcat actggaaagc ggttagaaga tgataaaaga gagattggca
     3901 atgatcttaa gttttattat gagaaatttg aaaaagtatt ttcatatatt tcaccggaac
     3961 agtggaaatt atttgatttt tgtggtaaag tgtcagccag attaagattt agcgatgcaa
     4021 tcattgaagc gtccgctgat ctggaacaat tattagacaa gaaccaatgg aagacatctg
     4081 nnnnnnnnnn ccagtgggat aatccaggaa tctggatgta tcatccaaag gttttatatg
     4141 atctgatcaa acgaaaggat tacctgaata cattgaatga tagaaaagat atatgcaatc
     4201 aggaattgat ccggcatgag cagattttag aaggaatcgg tgaacaagca gattccgaaa
     4261 ctatagaagg agtttagaga tgaagaaaga attagaaaca acgtatgatc caggtgcgat
     4321 tgaagaacgc ctgtatcaga aatggctgga taacaagtat ttccatgcag aagtagatga
     4381 gacaaaacaa ccatatacga tcgtgattcc acctccaaat atcacaggaa agcttcatat
     4441 gggacatgcg ttagatgaga cacttcagga cattctgatc cgttttaaga agatgcaggg
     4501 atataatgca ttatggatac caggaacaga ccatgcaagt attgcgacag aagtaaagat
     4561 catcaatgca ctgaaagaag aaggaatcga gaaggaagat cttggaagag agaagttctt
     4621 agaacgtgcc tgggattgga aagaagaata tggtggaacg atcatcgaac agatgaagaa
     4681 acttggatcc tcctgtgact gggatcgtga gagattcacg atggatgaag gatgttctga
     4741 agcagtcaaa gaagttttca ttaaattata tgaaaaaggt tatatttacc gtggatcaag
     4801 aatcatcaac tggtgtccgg tctgccagac atctatctct gatgcggaag tagaatacga
     4861 agatcaggca gggcatttct ggcatattaa atacccagtt gttggaagcg aaggtgaata
     4921 tgtagaaatc gcaacaacac gtccagagac attattagga gactctgcag ttgcagttca
     4981 tccagatgat gaaagatata cacatttagt tggtaagatg ttagaattgc cattatgcaa
     5041 tcgtcagatt ccagttgtag cagatgaata tgtagaccgt gagtttggaa caggtgccgt
     5101 taagatcaca ccagcacatg atccaaatga ctttgccatg ggtgaannnn nnnnnntgcc
     5161 agagatcaat atcttaaatg acgatgcaac gatcaacaag aatggtggta aatacgaagg
     5221 tatggatcgc tatgaagcaa ggaaggccat tgttgctgac ttggaagaac agggatattt
     5281 agttaagatc aaagaacatg aacataacgt aggaacacat gaccgctgcg gaacaacggt
     5341 tgagcctatg atcaagccac agtggtttgt gaagatggaa gaacttgcaa aaccagcgat
     5401 cgaagctgtg aagaacggag atctgaaatt cgtaccagaa cgttatacaa agacatatct
     5461 tcattggtta gagaacatcc gtgactggtg tatttccaga cagttatggt ggggacacag
     5521 aattccagca tattattgtg atgaatgtgg agaagttgtc gtagcaagag aaacaccgga
     5581 agtatgtcct aagtgtggat gcaagcattt cacacaggat gaagatacat tggatacatg
     5641 gttcagttct gcattgtggc ctttctcaac actcggatgg ccagagaaga caaaagaatt
     5701 agattacttc tatcctacaa atacactggt aacaggatat gatatcatct tcttctgggt
     5761 agtcagaatg gtattctcag gatacgaaca gacaggaaag agtccatttg acacagtact
     5821 gatccatgga ttagttcgtg atgaacaggg acgtaagatg agtaaatccc ttggaaatgg
     5881 tatcaatcca ttggatgtca ttgcacagta tggtgcagat gccttaagat ttacactgat
     5941 cacaggaaat gcccctggaa atgatatgcg ttacacagaa aaacgtgtta ttgcaagccg
     6001 taactttgca aacaagatct ggaatgcatc tcgtttcatt atgatgaact tagagcaggc
     6061 tgatttctct gatgttacat tagaagatct tacaagtgca gacaaatgga tcttatctaa
     6121 gttaaataca cttgccaaag atgttacaga caacatggag aaatacgaac ttggtatcgc
     6181 agttcagaaa ctaaacgact tcttatggga agaattctgt gactggtata ttgagatggt
     6241 aaaacctcgt ctgtacaatg atgaagacca gacaaaggca gcagcacttt ggacattaaa
     6301 tacagtttta acagaagcat taaaactgtt acatccatat atgccattta tcacagaaga
     6361 gatttattgt aatttaacag atgaagaatc tattatgtta gcaaaatggc cagag
//
LOCUS       DAOMUE010000102         6356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3312_length_6356_cov_2.193937, whole genome shotgun sequence.
ACCESSION   DAOMUE010000102 DAOMUE010000000
VERSION     DAOMUE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3312_length_6356_cov_2.193937"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..765)
                     /locus_tag="PLC71_05860"
     CDS             complement(43..765)
                     /locus_tag="PLC71_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRM02200.1"
                     /translation="MYELENDDEIEIDLRELFLALKKKIVWILLTAIVFAGASGLITK
                     FAMTPIYSSTAQLYVVSKGGFSQLTDLTMGTQLTQDYMVIVKTRPVLEQVIADLKLDM
                     DYKELENKITVENPSDTRIMQITITDKDAALAQSITQDLAEVTAKTVAEKMDVKSPTI
                     IEKAYKADKPDSPSLKKNVLIGAVLGFILMAAAIVIQYLMNDTILKEEDIEKYLGIIT
                     LAQLPLVKGTTKRTKKVKRARE"
     gene            complement(789..1499)
                     /locus_tag="PLC71_05865"
     CDS             complement(789..1499)
                     /locus_tag="PLC71_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRM02201.1"
                     /translation="MALIDIHTHILYGVDDGAKDINDSMGLLDEEREQGVDQVILTPH
                     YGPKFGHPDTEVLREKFEAIREKAYKYYPEIQLYLGSELYYQQGTVSDLKQGKALTMN
                     GTRYVLVEFATSDAYSHIYRAVQDFVYAGYIPILAHVERYKAVFGHVDKIVELIETGA
                     YIQINAESLIGGIFDKRASFCKKIMKEGLVHFLGTDCHDFRTRRPNMKPAAEVVRKKH
                     ADQILYENPRKMLEGKSI"
     gene            complement(1627..3222)
                     /locus_tag="PLC71_05870"
     CDS             complement(1627..3222)
                     /locus_tag="PLC71_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRM02202.1"
                     /translation="MANSIEQEIGRRRTFAIISHPDAGKTTLTEKFLLYGGAINTAGS
                     VKGKANSKHAVSDWMEIEKERGISVTSSVLQFEYEGKCINILDTPGHEDFSEDTYRTL
                     MAADCAVMVIDASKGVEPQTIKLFKVCVMRHIPIFTFINKMDREAKDTFDLLDDIEKV
                     LGIETCPINWPIGCGKEFKGVYERASRNILRFEAVSTGGAKEAKETIISADDDNAADL
                     IGEDFYENLMEEIELLDGAAADLDMEKVHQGKLSPVFFGSALTNFGVEPFLEHFLKMT
                     NRPLARTSKGEEVDPVENGFSAFVFKIQANMNKNHRDRIAFMRICSGKFEANMEVKHV
                     QSKKKMRLSQPQQIMAQERKIVEEAYAGDIIGVFDPGIFSIGDTVCLPKDDFEYEGIP
                     TFAPEHFARVIQLDSMKRKQFVKGVEQIAQEGAIQIFQEHEAGFAEIIVGVVGTLQFD
                     VLKYRLENEYNCEIRLEPLPYQAIRWIKDRNTDMNKLRGVSEVKKVKDMRGNPLLLFK
                     NEWGIQFVLDRNEGLELVEFSKE"
     gene            3425..4306
                     /locus_tag="PLC71_05875"
     CDS             3425..4306
                     /locus_tag="PLC71_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HRM02203.1"
                     /translation="MSNTILRNENVSVTLKSLGGELTSIKDASGTEYLWQGNPDFWSG
                     QAPVLFPIVGCLRNGTATIGNSKTCSFGRHGLARKLEFTLVSSSETCAVYSLKADDST
                     KEQYPYDFELQMIYELTDHGVKVSHKVINHGDIVMPYCIGGHPAFNCPVYEGENFEDY
                     IVEFEQPETAACAQLTDDGLINNNDRIPVLDHETVIPVKHSLFYKDALIFDQLKSRTV
                     ALKHKESGHGILVTFPDFDYLGVWSSANDGPFVALEPWSGTSTCSDEDDVFEHKRGVR
                     FLEPGEHETLSFVIEIL"
     gene            4412..5329
                     /locus_tag="PLC71_05880"
     CDS             4412..5329
                     /locus_tag="PLC71_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393234.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRM02204.1"
                     /translation="MNWHGKRYYSFDSFLKNYFSEKIYKVSLDGGFTCPNRDGTLGTG
                     GCIFCSEGGSGDFASSAALSVTDQITAGIEMVSRKIENGKYIAYFQAFTNTYGPIEKL
                     EALYMEAVNNPRIVALAIGTRPDCLPAEVLELLNQLNKIKPVFVELGLQTIHEETASF
                     IRRGYPLSCFDEAVMNLHKIGVLTVVHLILGLPGETDDMMLESVRYLNHLPIHGVKFS
                     MLHILKNTDLADHYEEHPFDVFTLESYVDLILKCIENLSPEIVIHRLTGDGPRDLLIA
                     PEWSVYKRKVLNRIAHEMKVKDVWQGDLL"
     gene            complement(5404..>6356)
                     /locus_tag="PLC71_05885"
     CDS             complement(5404..>6356)
                     /locus_tag="PLC71_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HRM02205.1"
                     /translation="EYKTFNQQLTILRNRGLIVPTDGKPKRFLEQENYYNVINGYKDL
                     FLLKDTHGKPVEPETYLSNAHFNELKTLFLFDRELRFLFLKYLLIFENSFKTVISHEF
                     SRKYPKANSYLDIANYREDDPKGVLKQISILTKTIHDNVGAKGAIKHYIEDHGSVPLW
                     VLVNYLTIGNLSYLYGALKDSEKNIIAKYYAEKYMKQYKNSKILRINSKDMESALKIF
                     NLVRNQCAHDERLYNSDYKNIRVSNIANYLEITNYNNRRIVVAILYLKILLNKDYYKK
                     FHSELNAIFKQYKNGFKTVSFEDILNIMGIDLLELDKLKN"
BASE COUNT         1762 a         1266 c         1087 g         2241 t
ORIGIN      
        1 taatcgttga tctctggaag atccattact aatttcttca tcttattccc tcgccctctt
       61 tactttcttt gttcgctttg tagttccctt taccaaaggt aattgcgcta aagtaataat
      121 tccaagatat ttctcaatat cttcttcttt caggatcgta tcgttcatca gatactgaat
      181 cacaatggct gctgccatca atataaatcc aagaactgct ccgatcaata cattcttctt
      241 aagacttggg ctgtctggtt tgtctgcctt atatgccttt tcgatgatcg ttggtgattt
      301 cacgtccatc ttctctgcaa ctgtcttcgc tgttacttct gcaagatcct gtgtgatgct
      361 ctgtgcaagt gctgcatcct tatctgttat cgtaatctgc atgatacgtg tatcagatgg
      421 attctccacc gtgatcttat tctctaattc tttataatcc atatcaagct tcagatctgc
      481 aatcacctgt tcaaggactg gtcttgtctt aacaattacc atataatcct gtgttaactg
      541 agtacccatt gtaagatctg taagctgtga aaatccaccc ttcgaaacaa cataaagctg
      601 tgctgtagaa gaatagatcg gtgtcatcgc aaacttcgtg atcaatcctg atgcacctgc
      661 aaagacgatt gctgtcaaca aaatccaaac gatcttcttc ttcaatgcca aaaacaattc
      721 ccttaaatca atctcgatct catcatcatt ctctaactcg tacatagatg tttttatccc
      781 ttctttaatt aaatactttt cccctcaagc atctttcttg gattctcata aagaatctgg
      841 tccgcatgct tcttccgtac aacttctgct gccggtttca tatttggtct tcttgtccgg
      901 aaatcgtgac aatccgttcc aaggaaatga accagtcctt ctttcataat cttcttacaa
      961 aaagatgctc gtttatcaaa aattcctccg atcagacttt ccgcatttat ctgaatatat
     1021 gcacctgttt cgatcagttc gacaatcttg tctacatgtc cgaaaactgc tttgtatcgc
     1081 tctacatgcg caaggatcgg aatatatcct gcatatacaa aatcctgaac cgcacgatat
     1141 atatgcgaat aggcatctga tgttgcaaac tctactaaaa cataacgggt cccattcatc
     1201 gtcaatgctt tcccttgttt cagatctgaa actgtccctt gctgataata taattcgctc
     1261 ccaaggtata gctgaatctc tggataatac ttgtatgcct tctctctgat cgcctcaaac
     1321 ttctctctca atacttctgt atccggatgt ccaaacttcg gaccgtaatg cggagtcagt
     1381 attacctgat ccacgccttg ttcccgttct tcatccaaca gtcccattga atcattgatg
     1441 tcctttgctc catcatcaac tccatataat atatgtgtat gaatatctat caacgccata
     1501 acaatctctt ttctaagtct gatatttata ttatttttga taacttaaga aatatactaa
     1561 tataatatac catctttatt ctcaagttac aatgcttttt cttaccaaaa agaaatacaa
     1621 tttatactat tctttgctga actcaactaa ctctaatccc tcattacgat caagtacaaa
     1681 ctggattccc cattcattct taaataacaa cagtggattt cctcgcatat ctttgacttt
     1741 cttaacttca gatacaccac gtaatttgtt catatccgtg ttgcggtctt tgatccagcg
     1801 gattgcctga tatggcaatg gttctaaacg gatctcgcag ttgtattcat tttccagacg
     1861 gtattttaag acatcaaact gaagtgttcc tacaactccg acgatgatct ctgcaaatcc
     1921 tgcttcatgt tcctggaaga tctggatagc accttcctgt gcgatctgtt ctacaccttt
     1981 cacaaactgc ttacgcttca tagaatctaa ctggatgact cttgcaaaat gttctggtgc
     2041 gaatgttggg attccttcat attcaaagtc atcttttgga aggcagacag tgtctccgat
     2101 agagaagatt cccgggtcaa atactccgat gatatctcct gcatacgcct cttctacgat
     2161 cttacgttcc tgtgccatga tctgctgtgg ctgggaaagc ctcatctttt tcttactctg
     2221 tacatgtttt acttccatgt tcgcttcgaa tttaccggaa cagattctca taaatgcaat
     2281 tctgtcacgg tgatttttgt tcatatttgc ctgaatctta aagacaaacg ctgaaaaacc
     2341 attttccaca ggatctactt cttctccctt ggatgttctt gcaagtggtc tgtttgtcat
     2401 cttcaggaaa tgctctaaga atggttctac accaaagttt gtaagtgctg atccaaagaa
     2461 tactggagat agttttccct gatgtacttt ctccatatca agatctgctg ccgcaccatc
     2521 taataattca atttcttcca tcagattctc atagaaatct tctccgatca aatctgctgc
     2581 attatcatca tctgctgaaa tgatcgtttc ttttgcttct tttgcaccac ctgtggagac
     2641 tgcctcgaat cgtaagatat ttcttgatgc acgctcgtat acacccttaa attcttttcc
     2701 acatccgatc ggccagttga tcggacatgt ctcgatccct aagactttct cgatatcgtc
     2761 taacaaatcg aaagtatcct tagcttcacg gtccatctta ttgataaatg taaagatcgg
     2821 gatatgtctc ataacacaga ccttaaataa cttgatcgtc tgtggctcca cacccttgga
     2881 agcatcgatc accatgacgg cacaatccgc tgccatcagt gtacgatacg tatcttctga
     2941 gaaatcctca tgtcctggtg tatcaaggat attgatacat tttccttcgt attcaaactg
     3001 taatacagaa gaagtgacag agatacctct ttctttttca atctccatcc agtcagatac
     3061 tgcatgtttg gagtttgcct ttcctttcac agaccccgct gtattgatcg ctcctccata
     3121 taaaaggaac ttctcagtca acgttgtctt accggcatcc ggatgggaaa tgattgcaaa
     3181 ggttcttctg cggccgattt cctgctcaat actgtttgcc ataatctttt tcctcttttc
     3241 tatttttatc tattatttta catttctacg catacttagt catattatca caccacttct
     3301 taaatttcta ctgattcttt ataaaaaata tgcttttaac tattgctttt ctcaagaaac
     3361 acaagtgttt ttcgatgtta attcatggta taatcatact atattaaatc taggaggata
     3421 tctcatgagc aatacaatct tacgcaatga aaatgtatct gtcactttaa aatctcttgg
     3481 tggggaactg acttctatca aagatgcttc tggtacggaa tatctctggc agggaaaccc
     3541 tgacttctgg agcggacagg cacctgtctt atttccgatc gttggatgtt taagaaatgg
     3601 aactgcaacc atcggtaatt ctaagacttg ttcttttggc cgtcatggtc ttgcaagaaa
     3661 attagaattt actttggttt cttcttctga aacttgtgct gtttattctt tgaaagcgga
     3721 tgattctacg aaagaacagt atccttatga ttttgaactc cagatgatct atgaattaac
     3781 ggatcatgga gtgaaagtca gtcataaggt gatcaatcat ggggacatcg ttatgcctta
     3841 ctgtattggc ggtcatcctg catttaactg tccagtgtat gaaggggaga attttgaaga
     3901 ttatatcgtg gaatttgaac aaccggaaac tgctgcctgt gcacagctta cagatgatgg
     3961 tttgatcaat aacaacgatc gtattcctgt tcttgatcat gaaactgtga tccctgtaaa
     4021 acattcttta ttttacaagg acgctttgat ctttgatcag ttgaaatcac gcacggttgc
     4081 attaaaacat aaggaaagcg gtcatggtat cttagttact ttccctgatt ttgattatct
     4141 gggtgtatgg tctagtgcaa atgatggacc ttttgttgct ttggaacctt ggagtggaac
     4201 ttctacctgc agtgatgagg atgatgtgtt tgaacataaa agaggggtgc gattcttgga
     4261 gcctggagaa catgaaactt tgagttttgt gattgagatt ttatagtacg tgataatgtt
     4321 atgaacattt tgtgacttga aaacgctgga atttccacat ttttcgtgct atcttgtaga
     4381 taacaaactt gtagttagga gttatgatca tatgaattgg catggaaaac gttattattc
     4441 gtttgatagt tttttaaaga attattttag tgagaaaatt tataaagtat ccttagatgg
     4501 tggttttacc tgtccaaaca gagatggaac acttggaact ggtggttgta tcttttgcag
     4561 tgagggcggt tccggggatt ttgcttcttc agctgctttg tctgtgacag atcagatcac
     4621 cgctggaatt gagatggttt ctagaaagat cgagaatggg aagtatattg cttattttca
     4681 ggcttttacg aatacttatg gtccgattga aaaactggaa gctttatata tggaagcggt
     4741 caataatccg cgaatcgttg ctcttgcgat cggtaccaga ccggattgct tgcctgccga
     4801 ggttttggaa cttttaaatc aattaaataa gatcaaacct gtgtttgtgg aacttggtct
     4861 gcagacgatt catgaagaga ccgcttcttt tatccggcgt gggtaccctt tgtcttgttt
     4921 tgatgaagca gttatgaatc ttcataagat cggtgtattg actgttgttc acctgattct
     4981 tgggcttcct ggtgaaacgg atgatatgat gcttgagtca gttcgttatc tgaatcacct
     5041 tccgattcat ggggttaaat tttctatgct gcatattttg aagaatacgg atctggctga
     5101 tcattatgag gaacatccct ttgatgtgtt tactttggag tcttatgttg atctgatttt
     5161 gaaatgtatt gagaatctga gtcctgagat cgtgattcat cgattaactg gagatggacc
     5221 gagagatttg ttgattgctc ctgagtggag tgtttataag cggaaagttt tgaataggat
     5281 tgcgcatgag atgaaggtga aggatgtttg gcagggggat ttgttgtaga ttgtaaccaa
     5341 ctttttaata tttttcaact attgtatgtt acacatagaa tacaaatatg gagactgttt
     5401 ttattaattt tttaatttat ctaattccaa taaatctatc cccataatat taagaatatc
     5461 ctcaaaactt actgttttaa atccattttt atattgttta aaaatcgcat tcaactctga
     5521 atgaaatttc ttatagtaat ctttattaag caatatctta agatataata ttgcaacaac
     5581 aatacgtcta ttattataat ttgtaatttc taaataatta gctatgttag atacacgtat
     5641 atttttataa tcagaattat aaagacgttc gtcatgtgca cactgatttc ttactaaatt
     5701 aaatatcttt aatgccgatt ccatatcctt actatttatc ctaagtatct tggaattttt
     5761 atattgtttc atatattttt cagcataata ttttgcgata atattttttt ctgaatcttt
     5821 taatgcacca tacaaatatg aaagatttcc tattgtaaga taattaacta aaacccataa
     5881 cggaacagaa ccatgatcct caatataatg ttttattgct cctttagcac caacgttatc
     5941 atgtatagtc ttggtaagaa tagaaatttg tttcaatacg cctttcggat cgtcttctct
     6001 ataattcgca atatccaaat atgaattagc ttttggatat tttcttgaaa attcatgtga
     6061 aataaccgtt ttaaaagaat tttcaaaaat aagtaagtat ttcaaaaata aaaatcttaa
     6121 ttccctatca aacaaaaata atgtttttaa ctcattaaaa tgcgcattgg acaaatatgt
     6181 ttctggctca acaggttttc catgtgtatc cttaagtaaa aacaaatctt tatatccatt
     6241 tatgacatta taataatttt cctgttccaa aaaacgttta ggttttccat ctgttggaac
     6301 aatcaaacct ctattacgca gtatagttag ttgttgatta aacgtcttat actctt
//
LOCUS       DAOMUE010000103         6102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3491_length_6102_cov_2.422689, whole genome shotgun sequence.
ACCESSION   DAOMUE010000103 DAOMUE010000000
VERSION     DAOMUE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3491_length_6102_cov_2.422689"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLC71_05890"
     CDS             complement(<1..371)
                     /locus_tag="PLC71_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRM02206.1"
                     /translation="MGKNLKKVIFSVLLLAAVIIVSPKDAKAAGKVWMAGFNDNSITI
                     QWTPQSLNGYRVDGYYLEKIGTQEMIWQGSADTTQVTVQATKGYSGYIRLGYRLTSLA
                     TGKTYNWSVDSVYVNTTPADVA"
     gene            complement(420..917)
                     /gene="queF"
                     /locus_tag="PLC71_05895"
     CDS             complement(420..917)
                     /gene="queF"
                     /locus_tag="PLC71_05895"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRM02207.1"
                     /translation="MSGRKSQELEGITLLGNQKTKYPDDYAPEVLETFENKHPENDYF
                     VKFNAPEFTSLCPITGQPDFATIYISYVPGERMVESKSLKLYLYSFRNHGDFHEDCMN
                     IIMKDLIKLMDPKYIEVWGKFTPRGGISIDPYCNYGRPGTKWEDVAWNRLSNHDLYPE
                     KVDNR"
     gene            complement(914..3775)
                     /locus_tag="PLC71_05900"
     CDS             complement(914..3775)
                     /locus_tag="PLC71_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM02208.1"
                     /translation="METLQVNRKQIEKLSIVLNNSRIKIFEYLVDEDTLVVYNNKFHI
                     EKTIAGYMDYIDNKSKIHPEDREKIKQIYKEAKEATVEIREFGENGDIKHSVLEFTKI
                     VNEDGKLTLIGSTRDITEQRKQEKKLKERARKDSLTGLYNQVYGKKCINKYLNRKKPF
                     DSCGMIVLDVDCFKLVNDNYGHSFGDKVLACLAMLLKEVFRDNDSILMRAGGDEFVIF
                     VKDILNIELVRKNVELMQKIRELQFGKTGCTITCSAGCCYLPENVSGYTYDQLFENAD
                     IALYKAKERGKNCYVYCDSLQHFSSMIAEQEKQEEMLEARYFQNDIVATAFEIFEKTN
                     NFEVAIHLLLKVIGVRLELDRITIVQTDIKAREIYSDYQWNREGIPKVLETVRHFDKE
                     DFLTVFNDFDENGVLVLQHDHMQRYSESATKILIQGEAKTIVCVAMYCEGRYTGAITY
                     AVCKEKRSWSNEMLKQLSEVTKIISAHFAKNQVMNHVYQGAITRMEHDTLTGLISFGR
                     FHEEVERIILANKTSDYMMIYTDFENFKYFNYKYGYTLGDQVLKDFCSFIIGKTEEKH
                     NLYFTRVVSDQFLMFRTASHEKDEYQGIIEEIEKINEEFMLRQKEKFPKSNVVLRTGI
                     YYVTPECRSTSYAIDAANYVRQKVKGGEKGSVRFYDDEMQKQRELENEIVNDMKEAME
                     QKQFKVYFQPQYSIKSHEIIGAEALVRWERDNGTVLSPNAFIPVYENNGKIIELDFYV
                     FETVVEFIAENLKAGREQVPISINASSLHASDPQTINTYINILKKYSVDPTMVEIELT
                     ETAVVSEYESVRKLFDSFQLHGIKTAMDDFGSGYSVLNTIVDIPVDVIKIDRGFITSC
                     LETDRGIYFLKHLIDMIRNLGYQIICEGVETDEQIEILRQIGCDEIQGYWYSKPLKME
                     DYKELLQTEKISRGAKHPNRQKILRYTLGNENLKVRKIQ"
     gene            4125..5543
                     /gene="hydG"
                     /locus_tag="PLC71_05905"
     CDS             4125..5543
                     /gene="hydG"
                     /locus_tag="PLC71_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HRM02209.1"
                     /translation="MYRPESGIAEEFIDDQEIKDTLAYADANKDNKELILSIIEKAKK
                     LKGISHREASVLLACEDKELIQKVFDLAIKIKEEFYGNRIVMFAPLYLANYCVNGCTY
                     CPYHYKNKHIRRKKLTQEEIKKEVIALQDMGHKRLALETGEDPVNIPIEYVLESIKTI
                     YSIKHKNGAIRRVNVNIAATTVENYKKLKDVGIGTYILFQETYHKDNYEVLHPTGPKH
                     DYAYHTEAMDRAMTAGIDDVGIGVLFGLDMYRYDFAGLLMHAEHLEAKFGVGPHTISV
                     PRIRPADDIDPDDFENAINDDIFEKIVAILRIAVPYTGIIVSTRETQESRERVLKVGV
                     SQISGASSTSVGGYAEKEKPEDNSAQFEKNDNRTLDEIIGWLMDLGHIPSFCTACYRE
                     GRTGDRFMTLVKSGQIANCCAPNALMTLKEYLEDYASDEVKAKGEVLIKQELEKIPNP
                     VVKQKATEYIENIHEGKRDFRF"
     gene            5553..>6102
                     /locus_tag="PLC71_05910"
     CDS             5553..>6102
                     /locus_tag="PLC71_05910"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HRM02210.1"
                     /translation="MFKHLNTRIPKRIEQEKDKVSAVMIPLIKKNDGYHILFEVRSNK
                     LSQQPGEICFPGGRREHDETSMETSIRETCEELLISESNIKLYGPLDYFLSPAGLRID
                     PYLGELVNYDGQYSNDEVSEIFTVPLAFFQNTPPKCYYNKVTMYPTDNFPFDKVPGGK
                     EYPWARGEYEVLFYEYDDRIIWG"
BASE COUNT         1764 a         1305 c          984 g         2049 t
ORIGIN      
        1 gctacatcag caggtgttgt gttaacataa acactgtcaa ctgaccagtt atatgttttt
       61 cctgtggcaa gacttgtaag tctgtatccc agtcttatat atcctgaata tccttttgtt
      121 gcctgaactg ttacctgcgt tgtatctgca gaaccctgcc agatcatttc ctgcgttcca
      181 attttctcaa gataatagcc atccactcta tatccattca aactctgtgg agtccactga
      241 atcgtaatgc tgttatcatt aaatccagcc atccatacct ttcctgctgc ctttgcatcc
      301 ttaggcgata caatgatcac tgctgccagc agtaaaacac taaatattac ttttttcaaa
      361 ttctttccca tactttttcc tccttttcgt atgtgattac ttttttcgta tgtgattact
      421 tatctattat ccaccttctc tggataaaga tcatgatttg aaagtctgtt ccatgcaaca
      481 tcttcccatt ttgttcctgg tcttccgtag ttgcagtatg gatcaatgga gattcctcct
      541 cttggagtga atttacccca tacttcaatg tattttggat ccatcagttt gatcagatct
      601 ttcataatga tattcatgca gtcttcgtga aaatctccat gatttcggaa gctgtataaa
      661 tacaatttta atgatttact ttctaccatg cgctctcctg gcacataaga aatgtagatc
      721 gttgcaaaat ccggctgtcc tgtgatcgga catagacttg tgaattctgg tgcattaaat
      781 ttgacaaaat aatcattctc tggatgttta ttctcaaatg tttctaacac ctccggtgca
      841 taatcatctg ggtattttgt tttctggttt cctaataacg taattccttc taattcctgg
      901 ctctttctgc cactcattgt attttcctca ctttcaaatt ttcattgcct aaagtatatc
      961 ttaggatttt ttgcctgttt gggtgttttg cccccctgga aattttttcc gtctggagaa
     1021 gttctttgta gtcttccatc ttcaatggct tcgaatacca atatccctga atctcatcac
     1081 aaccgatctg tcttaatatt tcaatctgct catctgtttc tacaccttca cagattatct
     1141 gatatcctag atttcgaatc atatcgatca aatgtttcag gaaatagatt ccacggtctg
     1201 tttctaagca gctggtaata aatcctcgat cgatcttgat cacatcaact gggatatcaa
     1261 cgatcgtatt caggacagaa tatccgcttc caaaatcatc catcgctgtc ttaatcccat
     1321 gtaactgaaa agagtcaaat aattttctaa cactttcata ttcagacaca actgctgtct
     1381 cagtaagttc aatctcaacc attgttggat caacactgta tttcttcaaa atatttatat
     1441 aagtgttgat cgtctgcgga tcagatgcat ggagactaga tgcattgatc gatatcggca
     1501 cctgttctct tcctgctttt agattctctg caatgaattc tacaactgtc tcaaagacat
     1561 agaagtcaag ttctatgatc tttccattat tttcataaac tggaataaat gcattgggac
     1621 ttaaaacggt tccgttatct ctctcccagc gcaccaatgc ttccgcacct atgatctcat
     1681 gactttttat ggaatattgg ggctgaaaat atactttgaa ctgtttttgt tccatcgctt
     1741 ccttcatatc attgacgatc tcattttcca actcacgttg tttctgcatc tcatcgtcat
     1801 aaaatcttac gctgcctttt tctccacctt tcactttctg tctcacatag tttgccgcat
     1861 cgatcgcata ggaagtgctc ctgcattctg gtgtcacata atagattcct gttctcaata
     1921 ccacgttgga ttttgggaac ttctccttct gccgcagcat aaattcttca ttgatctttt
     1981 ctatttcttc aatgatccct tgatattcat ccttctcatg gctagctgta cgaaacatta
     2041 aaaactgatc cgatacaact cttgtaaagt acagattatg tttttcttct gtttttccaa
     2101 taatgaaact gcaaaaatcc tttaatacct gatctcctag agtatatcca tacttataat
     2161 taaaatattt aaaattttca aaatccgtat atatcatcat gtaatcactt gtcttatttg
     2221 caagaatgat acgttccact tcctcatgaa atcttccaaa tgagatcaaa cctgtcagcg
     2281 tatcatgctc cattcgtgtg atcgctccct gatagacatg attcatcacc tgattcttgg
     2341 caaaatgtgc ggagatgatc ttcgttactt cggataactg cttcaacatc tcgtttgacc
     2401 agcttctctt ctccttacac acagcatatg tgatcgctcc cgtatagcgt ccttcacaat
     2461 acattgcaac acatacgatt gtctttgcct ctccctgtat caggatcttc gttgctgatt
     2521 cggaatatcg ttgcatatga tcatgctgta aaacaagcac cccattctca tcaaaatcat
     2581 taaatactgt gagaaaatcc tctttatcaa aatgtctgac cgtctctaaa accttcggaa
     2641 ttccttctct gttccattga taatcagaat atatctctcg agccttgata tctgtctgca
     2701 cgatcgtgat acgatcaagt tccagacgaa ctccgatcac tttcagtaat aaatgtatcg
     2761 ccacctcaaa attgttggtt ttctcgaaga tttcaaaagc tgtagcaaca atatcattct
     2821 gaaaatatct tgcttccagc atctcttcct gtttctcttg ttcagcgatc atggatgaaa
     2881 aatgctgcag gctgtcacag taaacataac agttctttcc tcgctctttt gctttatata
     2941 aggcaatgtc cgcattctca aacagttggt cataggtata acctgataca ttctctggaa
     3001 gataacaaca tcctgcacta catgtgatcg tacaacctgt cttcccgaat tgaagctccc
     3061 tgatcttctg catcagctcc acattcttcc taaccaactc aatgttcaag atatctttta
     3121 caaaaataac gaattcatcc ccgccagctc tcattaaaat actatcgtta tctctaaata
     3181 cttctttcaa cagcatcgcc aaacaggcta gaactttatc tccaaatgaa tggccataat
     3241 tatcattcac caacttaaaa caatccacat ctaatacaat cattccacac gaatcaaaag
     3301 gcttcttccg attcagatat ttgttgatac attttttccc atatacctga ttataaagcc
     3361 ctgtcaaaga gtcttttctc gctctttctt tgagcttctt ctcttgtttt ctttgttctg
     3421 tgatatccct tgtacttcct atcaaggtca gctttccatc ttcattcacg atcttcgtaa
     3481 actcgagtac cgaatgttta atatccccat tctccccaaa ctcacggatc tctaccgttg
     3541 cttcttttgc ttccttataa atctgcttta tcttttctcg gtcttctgga tgaatcttgc
     3601 ttttgttgtc tatatagtcc atgtatccag ctatcgtttt ctcgatatga aatttattat
     3661 tataaactac aagtgtatct tcatccacaa gatattcaaa tatcttaatc cgagaattat
     3721 ttaaaactat tgacagtttc tctatttgtt ttctgttaac ttgcagtgtt tccatatcct
     3781 attatttctc ccgtagtatt gctatttccc tattaccaaa agtatacaat gaaacgctta
     3841 gaaacacaat cttaatcgtt ctcttttttt ctttcattga ctttttgtct catttattta
     3901 taatgaatat ataagtgcat attcattctg aagccaagga tttcctgatg ccaagattga
     3961 gcttacaaag tacctgttcc cttggggatc ttacgtactt ttttattcga ctatctggca
     4021 tattcatttc cgaatatgct tttttattgc atagaaaata tcgataaaat ctcctatgca
     4081 ataaaaatat tacgtattaa ctaatttagg agaaggagaa aactatgtac agaccagaat
     4141 ccggtatcgc agaagaattt attgatgacc aggagatcaa agatacactt gcttatgcag
     4201 atgccaacaa agacaataag gaactgatcc tgtctatcat tgaaaaagca aagaaattaa
     4261 aaggtatttc ccacagagaa gcttccgtac ttcttgcatg tgaagataaa gaactgatcc
     4321 agaaagtttt tgatcttgcg atcaagatca aggaagaatt ctatggaaat cgtatcgtta
     4381 tgtttgctcc attatatctt gcaaactatt gtgtcaacgg atgcacttac tgtccttatc
     4441 attataagaa caaacatatc cgcagaaaga aattaactca agaagagatc aagaaagaag
     4501 ttatcgctct tcaggatatg gggcataaga gactggctct tgaaacaggg gaagatcctg
     4561 tgaatattcc aattgaatat gtattagaga gcattaaaac aatttacagt atcaaacaca
     4621 aaaacggtgc gatccgtcgt gtaaacgtaa atatcgctgc tacaacagtt gaaaattata
     4681 agaaattaaa agatgttggt atcggaacat atattttatt ccaggaaacc taccacaaag
     4741 acaattatga agttcttcat ccaacaggac caaaacatga ttatgcttac catacagaag
     4801 ctatggaccg tgcgatgaca gctggtatcg atgatgttgg tatcggtgtt ttattcggtc
     4861 ttgatatgta tcgttatgac tttgctggtc tgttaatgca tgcagaacat ctggaagcaa
     4921 aattcggtgt tggacctcat acgatcagtg ttccaagaat ccgtcctgct gacgatatcg
     4981 atcctgacga tttcgaaaat gcaatcaatg atgatatctt tgagaagatc gttgcaatcc
     5041 ttcgtattgc tgttccttat acaggaatca tcgtatctac tcgtgagaca caagaatcaa
     5101 gagaacgtgt attaaaagtc ggagtatccc agatcagtgg tgcttcttct acaagtgtcg
     5161 gtggatacgc tgaaaaagaa aaaccagaag ataactctgc acagtttgag aaaaatgata
     5221 accgtacctt agatgagatc atcggatggc tgatggatct tggtcatatt ccaagtttct
     5281 gtacagcatg ttaccgtgaa ggacgtactg gtgatcgttt catgactctt gtcaaatccg
     5341 gtcagattgc aaactgctgt gcacctaatg ctttaatgac tttaaaagaa tacttagagg
     5401 attacgcttc tgatgaagtc aaagccaaag gtgaggtatt gatcaaacaa gaacttgaga
     5461 agattccaaa tcctgttgtc aaacagaaag ctaccgaata tatcgaaaat attcatgaag
     5521 gaaaacgtga tttcagattc taggagattt ttatgttcaa acacttaaac acccgaattc
     5581 caaaaagaat tgaacaagaa aaggacaagg tatctgccgt tatgatccct ctgatcaaga
     5641 aaaatgatgg ataccatatc ctatttgaag tccgttccaa taaactctca caacagcctg
     5701 gggaaatctg tttcccgggc ggccggagag aacatgatga aacatccatg gagacttcga
     5761 tccgtgaaac ttgtgaagaa ttactgattt ctgaatctaa tattaaatta tatgggcctt
     5821 tagattactt tttatctcct gctggtctgc ggatcgatcc ttatcttggg gaattagtaa
     5881 attatgatgg tcagtatagt aatgatgaag tttctgaaat tttcactgtt ccattagcat
     5941 tctttcagaa caccccaccg aaatgttatt ataacaaagt gactatgtat ccaacagata
     6001 actttccatt tgataaagta cctggtggaa aagagtaccc ttgggcacgt ggggaatatg
     6061 aggttttatt ctacgaatat gatgaccgta tcatatgggg ac
//
LOCUS       DAOMUE010000104         6070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3513_length_6070_cov_2.674480, whole genome shotgun sequence.
ACCESSION   DAOMUE010000104 DAOMUE010000000
VERSION     DAOMUE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3513_length_6070_cov_2.674480"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="PLC71_05915"
     CDS             complement(<1..1086)
                     /locus_tag="PLC71_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRM02211.1"
                     /translation="MHFIEISNIFKSEKKRYKVTAVLKLLVMFALLGFFLSNKPMYHV
                     QTDFYLKRMGFVFLACFWMVFVTVCDIRLSQKVDRAFVIILGVIAPVMGWILSEFLIN
                     EAECAFPPTQFYQKEPLWIVLSLAMIILVDLLIIFLTNSIRVGSVIVPFVLFIFAVVV
                     CVVYEFRGIPMMAPDILTVQTATSVMGNYTFKLTFEQYSVILVCMAFFFTFLRLHEVK
                     ATEKRVFHIAGFIVVALGCGLFTNQIILSDFMEEHQINIRMFRPMESYQKYGGVLTFA
                     RSVGYAVVKKPEGYTTAKVDQIIQEYEKKSANEQQSTAKQYPNIITVVNETFADIKVL
                     GDFKTNEDYMPYFHSLKKNCVTGYTYAS"
     gene            complement(1226..>1477)
                     /locus_tag="PLC71_05920"
     CDS             complement(1226..>1477)
                     /locus_tag="PLC71_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HRM02212.1"
                     /translation="EERKKLVEAVRYVDLVIPEENWDQKISDVKEYHVDTFVMGDDWK
                     GKFDFLKDYCEVVYLPRTEGISTTKIKQDLGLNGTTANK"
     assembly_gap    1478..1487
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1488..1637)
                     /locus_tag="PLC71_05925"
     CDS             complement(<1488..1637)
                     /locus_tag="PLC71_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRM02213.1"
                     /translation="MRKVITYGTFDLLHAGHINLLRRAKELGDYLIVVVSTDEFNWNE
                     KRKKCY"
     gene            complement(1661..2401)
                     /locus_tag="PLC71_05930"
     CDS             complement(1661..2401)
                     /locus_tag="PLC71_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM02214.1"
                     /translation="MAKKEVLKVEHVGMKFNLSQEKVDDLKDYVIKLLKHQISYNEFW
                     ALKDINFTLNKGDRLGILGLNGAGKSTLLKVIAGVLKATEGTVTAKGKIAPLLELGAG
                     FDQQYTGRENIYLYGAVLGFSKKFLDEKLDEIIEFSELGKFIDVPVKNYSSGMKSRLG
                     FSVATLVEPDILILDEVLSVGDAKFRKKSEAKIMSMFDKGVTVLFVSHSLDQVKRLCN
                     KAILLEKGKIISHGSIEEVSKVYEEKTK"
     gene            complement(2411..3187)
                     /locus_tag="PLC71_05935"
     CDS             complement(2411..3187)
                     /locus_tag="PLC71_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM02215.1"
                     /translation="MKSYIENFKKYRFLLWELVKKGVKLKYRRSYLGILWTLLEPLMT
                     MIVLSVVFGTLFGNKDPHFPVYILCGRLMYSFFSSATNGAMKAIRTNAGMIKKVYVPK
                     YIYPLSSVLFNYIIFAISLIVLVVVSIVLKVYPTIYLAQAIIPLILLLITAFGVGMIL
                     STMAVFFRDLEYLWSVGLMIIMYASAIFYKPEKLLKSGFGWILKCNPLYLLIHNFRQA
                     VFGMPMNMKFLAASIVFAIVSVIVGLIFFYKKQDEFILHI"
     gene            complement(3261..4316)
                     /locus_tag="PLC71_05940"
     CDS             complement(3261..4316)
                     /locus_tag="PLC71_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRM02216.1"
                     /translation="MDRKRRIKYIDLCKGLGILMVTWGHITKLGNPVDTWAASFKMAI
                     FFVAAGYLIRYADSYRTQTLKGYAVKLLKSLMLPYVLFSILSIGFRFATMIMKHRIDI
                     PAIKSYILATITLRGTFALWFLPVLFIAEILFFCLIKYLPKWVRIVILIVIPVFGIWE
                     SYFIRHLIVSVDPLTFERISFLILPISKALIALWFLEIGHIGCMLFSKVTSREIRFMI
                     GLVFTIGNIFLSQQNNGVDLNNMSLGIQPPLFFVTGIIGSFGALFVFEFLEDYVTFTI
                     LNYFGKNSLILMCTQRPFYIISTATAGWKIISGMPGIMAWRYYIDCLGAMILVMIIEY
                     SIITFVNTKAKFLIGRF"
     gene            complement(4318..>6070)
                     /locus_tag="PLC71_05945"
     CDS             complement(4318..>6070)
                     /locus_tag="PLC71_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HRM02217.1"
                     /translation="MDELDKSSVYMTGIKKHDITYQNTFEPADTSVIPYMNEVDGRFS
                     VFMGDQAQLSVRNMRGQVLKFKIKGSKVHVKMKYKNKEAGIKNVILSYRSKVETIDYP
                     FEYKVTRSGDHYIIDAQIDMSVLSLEELFWDVFAVTEKNGEEVRVSAYWSRWQRLKLL
                     LMNYQCDVDKEHIIFPYSTITCKMAFTYRTRSKYDGFDVKIKELAAFGVYTLLLPYWK
                     KKRVWLVFEKFCSMAQDNGYYFFKYCMEQLPKEKKQHIYYILDTDSADYDKMKQYGKH
                     VIPFMSFRHILYSLVANLYIASDSKKHLYTWRAKPNVISNRISKHNILFLQHGVTALK
                     RVHPIFGMKGSSPMTHFTTTSRFEHKIIVENFGYEDGDAPILGFTRWDVLEDTSKPEE
                     KIILAMPTWRSWLEEKSAEEFKASDYYKNYMKLLQSQKLARILKENDVKLIFYIHPKF
                     KDYLSEFNVSGDNIELIPFGTEPLNEIMKKCSMLITDYSSVCWDVCYLDKPVLFYQFD
                     YDMYMQAHGSYLDMEHELFGERYTEYEKLIDGIEEYIHNGFKEKEEYTKLKDYYFEYR
                     DNDNSKRTYEYIISKGY"
BASE COUNT         1957 a         1292 c          769 g         2042 t
ORIGIN      
        1 cgatgcatac gtatatcctg tcacacaatt cttcttcaaa ctatggaaat agggcatata
       61 atcttcgttc gtcttaaaat ctcccagcac cttaatatct gcgaatgttt cattgacaac
      121 tgttatgata tttggatatt gctttgctgt actttgctgt tcattcgctg atttcttttc
      181 atattcctga atgatctgat caaccttcgc tgttgtatat ccttctggct tcttcacgac
      241 cgcatatcca acacttcttg caaatgtcag cacgcctcca tatttttgat aactttccat
      301 tggacggaac atacgaatat tgatctggtg ctcttccata aaatcagaca gtatgatctg
      361 atttgtgaac aatccgcatc ctaatgcaac aacaataaat cctgcaatgt gaaatactct
      421 tttttctgta gcctttacct catgaagtct taaaaatgta aagaaaaatg ccatacatac
      481 caagatcaca ctatattgtt caaacgtcaa tttgaatgta taattaccca tcacgctagt
      541 agccgtctgt actgttaaaa tatctggtgc catcatcgga atcccgcgaa attcatatac
      601 aacacataca actaccgcaa agataaacaa tacaaatggt acgatcactg atccaactct
      661 gatgctgttc gtcaaaaata tgatcagcag atccaccaaa atgatcattg caagacttag
      721 aacgatccaa agtggctctt tctgatagaa ctgtgtcggc ggaaatgcac actctgcttc
      781 attgatcaaa aattctgaca agatccatcc cataactggt gcgatcactc caagaatgat
      841 cacaaaagcc ctgtcaacct tctgactcaa acgaatgtcg caaactgtta caaataccat
      901 ccaaaaacat gccaaaaata caaatcccat acgtttcagg taaaaatcag tctgcacatg
      961 atacattggc ttatttgata aaaagaatcc aagcaatgca aacatgacca gtaattttaa
     1021 aactgcggta actttatatc tctttttttc acttttaaaa atattgctaa tttctataaa
     1081 atgcatcaaa aactaatcct ttcatctata gcattatctt aaattctttt tgtttgataa
     1141 cgcagaaaag aagaattccg tctctagccg ctcataacct ctattgtcaa cgaaattcct
     1201 cttatatctt aattcataac tcttgctatt tattcgctgt tgttccgttt aatccaagat
     1261 cctgtttgat ctttgttgta gaaattcctt ctgttcttgg aagatatacg acttcacagt
     1321 aatcttttaa gaaatcaaac ttacctttcc agtcatctcc cataacaaat gtatctacat
     1381 gatattcttt cacatcggaa atcttctgat cccagttttc ttctggaatc acaagatcca
     1441 cataacgaac tgcttctacc agtttttttc tttcttcnnn nnnnnnnata acattttttc
     1501 cgtttttcat tccagttaaa ctcatctgtt gatacaacta caattaaata atcacctaat
     1561 tcttttgctc tgcgcaataa attgatatgt cccgcatgca gtaaatcaaa tgttccatat
     1621 gtgattactt ttctcatatc acacctccgt gtcttctatc ttactttgtt ttctcttcgt
     1681 atactttact tacttcttca atcgatccat gagaaatgat ctttcctttt tcaagtagga
     1741 ttgccttatt acataatctc tttacctgat ctaaagaatg agatacaaat aatactgtaa
     1801 ctcccttgtc aaacatactc atgatctttg cttcactttt ctttcggaat ttcgcatctc
     1861 cgacagataa tacttcatcc aggatcaaga tatctggttc cactaatgtt gcaacagaga
     1921 atccaagtct tgatttcata cctgaagaat agttcttaac tgggacatct ataaatttgc
     1981 ccagttctga gaactctatg atctcatcta acttctcatc taagaacttc tttgagaatc
     2041 caagaactgc tccataaaga taaatattct cacgtcctgt atactgttga tcaaatccag
     2101 cacctaattc aagcaatgga gcgatcttcc ccttggctgt cacagttcct tccgttgctt
     2161 ttaatacccc tgcaataacc tttaacagcg tactctttcc agctccattc aatccaagga
     2221 ttccaagtcg atcccctttg ttaagggtaa aattaatatc ttttaatgcc cagaattcat
     2281 tatatgaaat ctgatgtttc aataacttga tcacataatc tttcagatca tcaacctttt
     2341 cctgacttaa gttaaacttc attccaacat gttcaacttt caatacttct ttctttgcca
     2401 tgataccctc ctaaatatgc agaatgaatt catcttgctt cttatagaag aaaatcaatc
     2461 ctacaataac acttacgatt gcaaacacga tggatgcagc taaaaacttc atattcatcg
     2521 gcataccaaa aacagcctgt ctaaaattat ggatcaaaag atataatgga ttacatttta
     2581 agatccatcc aaaacctgat tttaataact tctctggttt gtagaaaatt gctgatgcat
     2641 acataatgat cattaatcct acagaccata aatattctaa gtctctaaag aatactgcca
     2701 tcgtagatag gatcattcca actccaaaag ctgtgatcag taataagatc aatggaatga
     2761 tcgcctgtgc cagataaatg gttggatata ccttcaaaac aatagatact actaccaaca
     2821 cgatcaaaga gatagcaaaa ataatataat taaacaatac actggataat ggataaatat
     2881 attttggcac atatactttc ttgatcattc ctgcattcgt cctgatcgct ttcatcgcac
     2941 catttgtcgc agatgaaaag aaactataca tcaaacgtcc acataagata tatactggga
     3001 aatgtggatc cttgtttcca aacaatgtac caaatactac acttagtacg atcattgtca
     3061 ttaacggttc taacagtgtc cataaaatac ctaaatatga tcttctgtat tttaacttaa
     3121 cacctttctt tacaagttcc cataaaagga atctgtactt tttaaaattt tcaatataag
     3181 atttcactgt acttctccat tttgatattt ttaatttaca ccttgctatt atactattcc
     3241 ttaacagatt attcaactat ctaaaatctt ccgatcaaaa atttagcttt tgtattaaca
     3301 aatgttatta ttgagtattc aatgatcatc acaaggatca ttgccccaag gcagtcaata
     3361 taatatctcc atgccatgat ccctggcatt cctgatatga tcttccatcc tgcagttgca
     3421 gtagaaataa tataaaacgg tctttgtgta cacattaata tcaaagaatt ctttccaaaa
     3481 taattcagta tcgtaaatgt aacataatct tcaagaaatt caaagacaaa tagtgctcca
     3541 aaagatccaa tgattcctgt aacaaaaaat agtggtggtt gaatacctaa agacatatta
     3601 tttaaatcta caccattatt ttgctgtgat aaaaagatat ttccaattgt aaataccagt
     3661 ccgatcataa atcggatttc tctggatgtt actttactga ataacataca tccaatatgt
     3721 cctatctcca agaaccataa tgcgatcaat gctttactga tcggtaagat caaaaatgat
     3781 attctttcaa atgttaatgg atccactgaa acgatcaagt gtctgataaa ataagactcc
     3841 caaattccaa aaacaggtat cacgatcaat atcacgatcc tcacccattt cggaagatat
     3901 ttgataagac agaaaaacag gatctccgca ataaataaca ccggtaaaaa ccatagtgca
     3961 aatgttcctc ttaatgtgat cgttgccaaa atataagatt tgatcgccgg aatatcaatt
     4021 ctgtgtttca tgatcatcgt tgcaaatcga aagccaatcg acaaaatact aaacaataca
     4081 tatggcagca tcaatgactt caataacttt actgcatacc cctttaatgt ctgtgtccga
     4141 taagaatctg cataccggat cagatatccg gcggcaacaa agaaaattgc catcttgaat
     4201 gatgccgccc atgtatcaac tggattacca agcttcgtaa tatgtcccca tgttaccatc
     4261 aagattccta agcctttaca cagatctata tattttattc tcctctttct gtccatttta
     4321 atatcctttg cttatgatat attcatacgt acgtttactg ttatcgttat cacgatattc
     4381 aaaataataa tcttttaatt ttgtatattc ttctttttct ttaaatccat tgtgaatata
     4441 ctcttcaatt ccatcgatca atttctcata ttctgtataa cgttctccaa atagttcatg
     4501 ctccatatcc agataacttc catgcgcctg catatacata tcataatcaa actgatagaa
     4561 taatacgggt ttatcaagat aacatacatc ccagcataca ctagaataat ctgtgatcag
     4621 catagaacat ttcttcatga tctcatttaa tggctctgtt ccaaatggga tcaactcaat
     4681 gttatctcca ctgacattaa actcgctcaa ataatcttta aactttggat gaatgtaaaa
     4741 gatcagcttt acatcatttt cttttaagat tcttgcaagt ttctgactct gtaacagttt
     4801 catatagttt ttataataat cacttgcctt aaactcttct gcactctttt cttccagcca
     4861 tgatctccat gtcggcattg caagaatgat cttttcctct ggttttgaag tatcttccag
     4921 aacatcccat ctcgtaaatc caagaatcgg tgcatctcca tcttcatatc caaaattctc
     4981 tacaatgatc ttatgctcaa atcttgatgt cgttgtaaaa tgagtcatag gacttgaacc
     5041 tttcattcca aaaatcggat gtacacgttt taatgctgtc accccatgct gtaaaaacaa
     5101 gatattatgt tttgaaatac gatttgagat cacatttggt tttgctctcc atgtatataa
     5161 atgtttcttt gaatcagaag ctatatataa attagcaaca agtgaatata aaatatgtcg
     5221 aaaactcata aatggaatca catgttttcc atattgcttc atcttatcat aatcagcaga
     5281 atctgtatcg agaatataat aaatatgctg cttcttttct tttggaagct gctccataca
     5341 atatttgaag aaataatatc cattatcctg cgccatagag cagaattttt caaatactaa
     5401 ccatactcgt ttctttttcc aatatggtaa taacaacgta taaacaccaa aagctgctaa
     5461 ttccttgatc tttacatcaa atccatcata ttttgatctt gtacgataag taaatgccat
     5521 tttgcatgtg atcgtcgaat aagggaaaat aatatgctct ttatcaacat cacattgata
     5581 attcatcagt aataatttta atctctgcca tctgctccaa tatgcactta ctcttacttc
     5641 ttctccattc ttctctgtta cagcaaatac atcccaaaac agttcttcca gtgataatac
     5701 cgacatatca atctgagcat caataatata atgatcaccc gacctcgtta ctttatattc
     5761 aaatggataa tcgatcgtct ccactttact tcgatatgat aatatgacat ttttgattcc
     5821 agcttctttg tttttatatt tcattttcac atgaactttt gaacccttga tcttgaattt
     5881 cagtacttgt ccacgcatat tacgaacgga taactgtgcc tgatctccca taaatacaga
     5941 aaatcttcca tctacttcat tcatataagg aatcacactt gtatccgcag gttcgaatgt
     6001 attctgataa gtaatatcat gtttcttgat tcctgtcata tatacagaag atttatctaa
     6061 ttcatccatc
//
LOCUS       DAOMUE010000105         6001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3571_length_6001_cov_3.009250, whole genome shotgun sequence.
ACCESSION   DAOMUE010000105 DAOMUE010000000
VERSION     DAOMUE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3571_length_6001_cov_3.009250"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /gene="srtB"
                     /locus_tag="PLC71_05950"
     CDS             <1..636
                     /gene="srtB"
                     /locus_tag="PLC71_05950"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HRM02218.1"
                     /translation="LYGYYKSYKEAKDTYEKIKKDNVKKTNGDRTIDFDKLRAKNPDI
                     VGWVYAKGTGIDYPIVQGKDNEEYLHMDYNKKKSSSGTIFLDHGCDKSFISDNNIIYG
                     HHMKNGTMFAKLLKFREESFLKKHHVIILYTPKKTMHLKVISAYAVKAQDQMPITFAN
                     ETQKKEYITKIRRMSEPSIKLDDKKIDCIYTFVTCSYERDDNRTYVHAVEE"
     gene            665..1432
                     /locus_tag="PLC71_05955"
     CDS             665..1432
                     /locus_tag="PLC71_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HRM02219.1"
                     /translation="MKKMKLTKKNKKELKMICGVLIFFAVIFIWIVQNIGLPIKTQVE
                     KGRIKVIDISSYNGTVNWKKVKDHNVNHAMIKIGSGMNEKRAGRKDSKFDTNFRNAGY
                     ASIHRGVYYYSYAKTTSDAKKEARHCLKLLKEQGIDPTDLDLPVAFDIEEEAVFQTGR
                     RNVTAVTTTFCDEIKKAGFEPMVYSGASALRNYFEYSKIREYKIWVAHYTKASAPAIP
                     FSYDMWQYTSRAVINGANTGMGYCDLNYYLVDKNYKE"
     gene            complement(1482..2393)
                     /locus_tag="PLC71_05960"
     CDS             complement(1482..2393)
                     /locus_tag="PLC71_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HRM02220.1"
                     /translation="MKYIKSAGCFSLFYVILYFILSTGMGIFITNTYLFTTLFYGILL
                     IAAFLYLKICGDDTKKTLRMHKIHIGSFFLVILLAFTIRPVAGFITMIANLFFQDVTT
                     NTMTQKVMQSLGLSVFTTAFLPGLVEEILFRGVLYSRLRKANPIKGILLSALLFGIAH
                     MNFQQFSYAFFLGIVFGCLLEATDSIFATITAHMIFNGSSILLTYAISKVSLFGVNNL
                     DTANTVTVSSIIASIPVALIGLILSIFLLVAIAYLNERLGYIKTWFQKDIRQTWPKKR
                     VTNISFWAAFIVCFLFSIMMEITSSFL"
     gene            complement(2406..3242)
                     /locus_tag="PLC71_05965"
     CDS             complement(2406..3242)
                     /locus_tag="PLC71_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HRM02221.1"
                     /translation="MIQLGKFQDLYIVKKKEFGVYVNDQKDVTDGSILLPAKQVPDGA
                     RIGDQISCFVYKDSEDRPIATVHIPKITLGAIRPLRVKEVSKIGAFLDWGLEKDLFLP
                     FKEQLGHIRPNKEYLVSLYIDKSDRLCATMKIGKLLSTDHHFKVNDWVHATVYNINPD
                     HGAFVAVEDQFLGRIPKREIHNKIVIGEQLNLRVTKVNEDGKLSLSPHEKAYLQIDRD
                     AKLIMDTIESYDGRLPFNDKARPATIERELGLSKAAFKRAVGRLLKDGLITITDNGIL
                     KK"
     gene            3481..3756
                     /locus_tag="PLC71_05970"
     CDS             3481..3756
                     /locus_tag="PLC71_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRM02222.1"
                     /translation="MNKTELVAAIAKKTELSKKDAEKALKAFTDVVAEELKKGEKIQL
                     VGFGTFEVSERAAREGRNPRSGEVMHIAASKAPKFKAGKALKDIVNE"
     gene            3860..4099
                     /locus_tag="PLC71_05975"
     CDS             3860..4099
                     /locus_tag="PLC71_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003504841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRM02223.1"
                     /translation="MRLDKFLKVSRLIKRRTVANEACSAGRVLVNGKAAKAGTNVKPG
                     DRIDIQFGNRETAVEVVSIAETVKKDEAADLYKFL"
     gene            4158..4442
                     /gene="yabP"
                     /locus_tag="PLC71_05980"
     CDS             4158..4442
                     /gene="yabP"
                     /locus_tag="PLC71_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HRM02224.1"
                     /translation="MIEERQPLRAHKISLSNRSLGVITGVKDVVSFDLNEIILDTDQG
                     LLMIKGKNLHVKKLLVEKGEVEITGKIDSFLYSNIEEPGKKEGLLGRLLK"
     gene            4442..4918
                     /locus_tag="PLC71_05985"
     CDS             4442..4918
                     /locus_tag="PLC71_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HRM02225.1"
                     /translation="MKETVTDPLLFFLASILFGVCLQAIYDIIRGIRRVFYHKSVFLF
                     IEDIGFGIFTAVMAFIFLCTYNDGELRGFFFLGIVIGMILYYQLCSKFLLRAVIHITE
                     KLKKIIRQFEKVCLRPGIHIKRNLKWQLKKEKKQVTMALKKQIKRGGKSDNTKETK"
     gene            4893..5192
                     /gene="ftsL"
                     /locus_tag="PLC71_05990"
     CDS             4893..5192
                     /gene="ftsL"
                     /locus_tag="PLC71_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HRM02226.1"
                     /translation="MTIRKKQNKLDQRTKIIMIIVLIFVVILAFSYINLRQKSKKIAK
                     EQQQVESQIKKQEEKKKELKKKKEYIKTKEFIEKTATEKFGLLYPGEYLLKGSDD"
     gene            5389..>6001
                     /locus_tag="PLC71_05995"
     CDS             5389..>6001
                     /locus_tag="PLC71_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM02227.1"
                     /translation="MEISNKRCIQILKLLLKNKECMTGDNLAVAIGVSSRTIRNDMKD
                     LNRALEDHGASVVSEIGQGYYLKIEDQEKFLTFQNEMEEHKREKDFKNIIPSEPEDRV
                     KYIISKLLLSTLNGRKEKIEFFDLEEELFISTSTLKKDFRNIDKVLKPYDLKTSITKK
                     DGVKIVGDEAKIRYCISEYIFNSKGYFGIEENQFYQSIFKEQEI"
BASE COUNT         2239 a          885 c         1227 g         1650 t
ORIGIN      
        1 ctgtatggat attataaatc ttataaagaa gcgaaagata cttatgaaaa gataaaaaaa
       61 gacaatgtaa agaagacaaa tggagataga acgattgatt ttgataaact tcgggcaaag
      121 aatccagaca ttgttggctg ggtttacgca aaggggacag gaattgatta tccgatcgtt
      181 caaggtaaag ataacgaaga atatcttcat atggattaca ataagaaaaa aagcagttca
      241 gggacgatct ttttagatca cggatgtgat aagtcgttta tctcagataa taatatcatt
      301 tatggacacc atatgaaaaa tggaacgatg tttgccaagc tgctaaaatt cagagaagaa
      361 tcctttctaa agaaacatca tgtgatcatt ttatatactc caaagaagac aatgcatttg
      421 aaagtgatca gtgcttatgc ggtgaaagca caggatcaga tgccgatcac atttgcaaat
      481 gaaacgcaga agaaagaata tatcacaaag attcggagaa tgagtgaacc atcgattaag
      541 ctggatgata agaagatcga ttgtatttat acatttgtga catgcagtta tgaaagagat
      601 gataatagga catatgtaca tgcagtggaa gaatgacaag gatgatagaa agtgttagat
      661 caagatgaaa aaaatgaaac taacaaagaa gaataaaaaa gaattaaaga tgatctgtgg
      721 agttttaatc ttttttgcag tgatctttat atggatcgta cagaatatcg gtctgccgat
      781 caagacacaa gtagaaaaag gccggatcaa ggtgatcgac atatcatcct ataacgggac
      841 agtcaattgg aagaaggtca aagaccataa tgtcaatcat gcgatgatca agatcggttc
      901 agggatgaat gagaagagag caggaaggaa agacagtaaa tttgatacca atttccgcaa
      961 tgcaggatat gcatcaattc acagaggcgt ttattattat tcttatgcga agacaaccag
     1021 tgatgcaaaa aaagaagcca gacactgctt aaaactcctc aaagagcagg gaatcgatcc
     1081 gacagattta gatctcccag tggcatttga tatcgaagaa gaagcggtat ttcagacagg
     1141 aagacgtaat gtgacagcag tcacgacaac attttgtgat gagataaaga aagcaggatt
     1201 tgagcctatg gtttattcag gagcttcagc acttcgaaat tattttgaat atagtaagat
     1261 cagagaatat aagatctggg tggcacatta tacgaaagca tccgcaccag caatcccatt
     1321 ttcttatgat atgtggcagt atacaagcag agctgttata aatggggcta atacaggaat
     1381 ggggtattgt gatctgaatt attatctggt agacaagaat tacaaagaat aaaagaactt
     1441 cagaatatga aagaaataac atattctgaa gttcttttgg tttaaagaaa agatgatgta
     1501 atctccatca tgatcgaaaa gagaaaacaa acaataaatg cagcccagaa agagatgttt
     1561 gtaacacgtt tctttggcca ggtctgtcgg atatctttct gaaaccatgt ttttatatag
     1621 ccgagccgtt cattcagata agcaatcgca acgagcagaa agatactaag aataagtccg
     1681 atcaatgcaa caggaatgga tgcaatgatc gaagataccg ttactgtatt tgcagtatca
     1741 agattgttta caccaaaaag tgaaactttt gatattgcat aagttaaaag gatggaagat
     1801 ccattaaaaa tcatgtgagc agttatggtt gcaaaaatgg aatctgttgc ttctaataag
     1861 catccaaata cgatacccaa aaagaatgca taggaaaact gctgaaagtt catatgagct
     1921 ataccaaaaa gcaaagcaga taataagatt cctttgatgg gattagcttt tcgcagtctg
     1981 gaataaagta ctccacggaa cagtatctct tccacaagac ctggaagaaa agcggttgta
     2041 aaaactgata atccaagact ctgcatcact ttctgtgtca tagtgttggt tgtaacatct
     2101 tgaaaaaaca aattggcgat catagtaata aaccctgcaa ctggacggat cgtaaaggca
     2161 agaaggatca cgagaaaaaa tgaaccaata tggatcttat gcattcgtaa tgtcttcttt
     2221 gtatcatctc cacagatctt aagataaaga aaagcggcaa tgagtaaaat accataaaat
     2281 aaagtagtaa aaagatatgt gttggtaata aaaatcccca ttccagtgga aagtataaaa
     2341 tacaagatca cataaaacag gctgaagcag ccagcggatt tgatatattt catggagtta
     2401 cctcgctatt tttttaagat tccattgtct gtgatcgtga tcagtccgtc ctttaacagg
     2461 cgtccaactg cacgtttaaa agcagccttg ctaagtccaa gctcacgttc gatcgtagca
     2521 ggtctcgcct tatcattaaa tggaagacgg ccatcatagc tttcaatggt atccatgatt
     2581 aatttcgcat cacgatcaat ctgaaggtaa gctttctcat gagggcttag ggataattta
     2641 ccatcttcat tcacttttgt aacacgaagg ttaagctgtt caccaattac gattttattg
     2701 tgaatctcgc gttttgggat acgtccaagg aactgatctt ctacagcaac aaaagcacca
     2761 tgatcagggt tgatattata aacagtggca tgcacccaat cgttgacttt aaaatgatga
     2821 tctgtcgata ataatttacc aatcttcatt gttgcacaaa gacgatcact cttatcaata
     2881 taaagagaaa ccagatattc tttatttgga cggatatgtc ctaactgttc tttaaatgga
     2941 agaaacagat ctttttctag tccccagtca aggaaagcac caatcttgct aacttctttg
     3001 acacgtagag ggcggatcgc acctaatgtg atctttggaa tatgaacggt agcaattggg
     3061 cgatcctcag agtccttata aacaaaacat gaaatctgat caccgatcct tgcaccatct
     3121 ggaacttgtt tggcaggaag aaggatactt ccgtctgtta catccttttg gtcattgaca
     3181 taaacaccaa actccttttt cttcacaata tataaatctt gaaattttcc taattgaatc
     3241 atatttatcc tctttcttaa tttttagatt tgtcagacac ttaatcgttg atttttgtcg
     3301 ttttcacaca ttataacaca gttccataaa aaagcgaaag aaaaagcctg aaaacagcat
     3361 aaaaacgtgg tttatttgaa gtttttactt gacttagatg aggaaagcct atagaattat
     3421 cttatgcaaa ttcaaagaca tatacaatta tgtttgagtt gagattagga ggaaaattta
     3481 atgaacaaga cagaattagt tgcagcaatt gcaaagaaaa cagaattgtc taaaaaagat
     3541 gcagaaaagg cattaaaagc ttttacagat gtagttgcag aagaattaaa gaaaggtgag
     3601 aagatccagt tagtaggatt cggaactttt gaagtatctg aaagagctgc aagagaagga
     3661 agaaaccctc gcagcggaga agttatgcac attgcagctt ctaaagcacc taaattcaaa
     3721 gcaggaaaag ctttaaaaga tattgtaaac gaatagaatg ttcatttgat attacattaa
     3781 ctgccggttt ctatggatcc ggcagttttt ctatatctat ttcagcaata taacatagaa
     3841 gaaagaagga gacataatca tgcgtttaga caagttttta aaagtatcca gactgatcaa
     3901 gagaagaact gttgcgaatg aagcctgtag tgcaggaaga gttctagtga acggcaaagc
     3961 agcgaaagct ggaacgaatg tgaaaccagg agacagaata gatatccagt ttggaaacag
     4021 agagacagca gtagaagtag tttctatagc agagacagtg aagaaagatg aggcagcaga
     4081 tctgtacaag tttttatagg tatgttttat aagctttgtt cataagatgt ataaaacatt
     4141 ttgggaggaa gtttagtatg atagaagaga gacaaccttt aagagcacat aagatcagct
     4201 tatcaaaccg atctcttggc gtgatcacag gggtaaaaga tgttgtttct tttgatctta
     4261 atgagatcat tcttgacaca gatcagggac tgttaatgat caagggaaag aatctccatg
     4321 taaagaaatt actggttgag aagggtgaag tagagattac gggaaagatt gacagttttt
     4381 tatactctaa tatagaagag ccaggaaaga aagaaggatt attaggaagg ttactgaaat
     4441 aatgaaagag actgtaaccg atccgttgtt attttttctt gcgtcaattt tatttggtgt
     4501 atgtttgcag gcaatatatg atattattcg tggaattcga agagtattct atcataaaag
     4561 cgtgtttttg tttatagaag atataggatt tggaatattt acagcagtga tggcatttat
     4621 atttctttgt acatataacg atggcgagtt aagaggattc tttttcctcg gaattgtgat
     4681 cggaatgatt ctatattatc aattatgcag caagttcctt ttgagggctg tcatacatat
     4741 tacagagaag ctaaagaaga tcataaggca atttgaaaaa gtatgtttaa gaccaggaat
     4801 tcatatcaag agaaacctga aatggcaatt gaaaaaagaa aaaaaacaag ttacaatggc
     4861 actaaagaaa caaataaaaa gaggtggtaa aagtgacaat acgaaagaaa caaaataagc
     4921 tggatcagag gacaaagatc atcatgatca ttgttctgat atttgtagtc attttagcat
     4981 tttcctatat caatctacga cagaaatcaa agaagatcgc caaagagcag cagcaggtgg
     5041 aatcccagat caagaaacaa gaagagaaga aaaaagaatt aaagaagaaa aaagaatata
     5101 tcaagacaaa agaatttatt gaaaagacag ccacagagaa gtttggacta ttgtacccgg
     5161 gagagtactt actcaaagga tcagatgact gataaacaac agaattaaaa aatttagggg
     5221 gggggaacaa acgttcctcc cttcgttttg cttataatgt gaaagacgaa attttagcta
     5281 aagataaaag gcggaaattt cgtctttttt ttccgcctta agtggaaaaa gacgaactat
     5341 attcttatgt gcatattgca tataatttag tacagaagga gaaaagtaat ggaaatatca
     5401 aacaaacgat gtatccagat tctgaaatta ttgttgaaaa ataaagaatg tatgacagga
     5461 gataatcttg cagttgcaat cggagtgtct tcaagaacca ttcgaaatga tatgaaagat
     5521 ttaaatagag cattggaaga tcatggtgca agcgttgtat cagagattgg acaaggatat
     5581 tatttaaaga ttgaagatca ggaaaaattt ttaacatttc agaatgagat ggaagagcat
     5641 aaaagagaaa aagattttaa aaatattatt ccatcagagc cagaagacag agtaaagtat
     5701 attatttcaa agttattgct gtcgacactc aatggaagaa aagaaaaaat agagtttttt
     5761 gatctggaag aagaactttt cattagtact tctactttaa agaaagactt tcgcaatatc
     5821 gataaagttt taaaacctta cgatcttaaa accagtatca caaagaaaga tggtgtgaag
     5881 atcgtaggag atgaggcgaa gatacgatat tgtatttcag aatatatctt taacagcaaa
     5941 gggtattttg gaatagaaga aaatcaattt tatcaaagta tttttaaaga acaggaaatt
     6001 c
//
LOCUS       DAOMUE010000106         5999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3574_length_5999_cov_2.491588, whole genome shotgun sequence.
ACCESSION   DAOMUE010000106 DAOMUE010000000
VERSION     DAOMUE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3574_length_5999_cov_2.491588"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..2753)
                     /gene="ppdK"
                     /locus_tag="PLC71_06000"
     CDS             complement(129..2753)
                     /gene="ppdK"
                     /locus_tag="PLC71_06000"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRM02228.1"
                     /translation="MATKWVYKFSEGNAEMRNLLGGKGANLAEMTGLNLPIPQGFTVT
                     TEACTNYYDCGGKISDEVASQVIEAMKDLEEIQGKKFGDLEDPLLVSVRSGARVSMPG
                     MMDTILNLGLNDEAVEGFAKKTGNPRFAYDSYRRFIQMFSDVVKEVDKAKFEDVLDDI
                     KAEKGVKFDTDLDADDLKEVIKRYKGIYRKALNEEFPQDPKDQLFEAIKAVFRSWDNP
                     RAIYYRRMNDIPGDWGTAVNVQTMVFGNMGDTSGTGVAFTRNPSTGEKQIYGEYLINA
                     QGEDVVAGVRTPQPITKLAEDLPECYEQFMSIAHTLEEHYRDMQDMEFTIQEGKLYFL
                     QTRNGKRTAPAALRIACELVDEGKITKEEAVSRIDAKALDQLLHPNFDQAALKAALPI
                     GEALPASPGAAAGKVYFDAEMAKLHHEAGLKVILVRLETSPEDIEGMHAAEGILTARG
                     GMTSHAAVVARGMGTACVAGCGDIKFAEDGKSFTLGGKTVQEGDYISLDGSTGKIYLG
                     EIRTVPASISGNFDRIMTWADEIRTLQVRTNADTPADALNAVKFGAQGIGLCRTEHMF
                     FDAERIPKIRRMILSTTKEAREIALNQLIPYQKKDFKDLYEVMEGRPVTIRFLDPPLH
                     EFLPNTMEEITALAKDMGVTVEEINMRRAALHEFNPMMGHRGCRLAVTYPEIAKMQTR
                     AVMEAAIEVKQEKGYDIVPEIMIPLVGEKKELAYVKEVVVQTAEKVKAYYESDIKYKV
                     GTMIEIPRAALLADEIAEEAEFFSFGTNDLTQMTFGFSRDDAGKFLESYYENKIYESD
                     PFAKLDQKGVGQLIEMAAKKGKATRPDIHLGICGEHGGDPSSVEFCYRAGLDYVSCSP
                     FRVPIARLAAAQAVLKDK"
     gene            complement(3122..4579)
                     /locus_tag="PLC71_06005"
     CDS             complement(3122..4579)
                     /locus_tag="PLC71_06005"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRM02229.1"
                     /translation="MEEMKVTKFPEGFLWGSASAAYQIEGGWREDGKGITNWDQFVRI
                     PGKTYKATTGDVAVDHYHRYKEDIALMAEMGLKTYRFSVSWARIYPEGRGEVNPKGIE
                     FYENIIDECLKYGIEPMVTIYHWDLPQALVDLYGGWESEEIIEDYVNYAKTLFKAYGS
                     KVKYWITFNEQNIFTSLGWLTAQHPPGKFDDQKTFYQVNHHVFMAHAKAVLAYREMGG
                     TGKIGASFAYTPSYALDCKPENVMSKHDYDELKNFWWMDVYAYGRYPKAAMNYLRKKG
                     FAPVVTKDDQKILKAAAAEIDFMGVNYYQSCVCEYNPMDGVTPYGTMNTTGVKGSAQV
                     LGVPGIYKNPGNPYLMTTDWDWSIDPVGLRYCCREITSRYDLPIIISENGLGAFDKKT
                     EDHKIHDEYRIDYLREHLKELGKAIEEGCEVLAYCTWSFTDLLSWLNGYQKRYGFVYV
                     DREEEEGATLDRYKKDSFYWYQDVIKQDGENLFKE"
     gene            complement(4601..5731)
                     /locus_tag="PLC71_06010"
     CDS             complement(4601..5731)
                     /locus_tag="PLC71_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HRM02230.1"
                     /translation="MLVVLIEAPIALIILGPLGAICGNGLSTVVYAIHDKLGFIAIGL
                     VAGVYPFVVMAGMHHAFTPIKLGMIATTGYENFICIGELCSNMAQGAASLAVALRSKN
                     KDFKQIAGSSAFSALFAGITEPALYGVTLRLKRPMLGACIGGAVGGLVGGFFQMKCFG
                     IATPAIVTIVQYVEKGKPQTLLFAALTILVTVVVTFVATLIIGFEDIVDEDDDLDFVE
                     ESNAQLLDNEISITSPLEGKVIPLTEVKDPTFSQEILGKGAAIIPEKGVVYAPFDGKV
                     DAVFETGHALGLVSEDGVELLVHVGIDTVNLKGKYFTPKKKSGDTMKKGDILLEFDID
                     KIKADGYDVTTPIIISNTEQFAKVKACEDKVVTKKSKLLSVQ"
     gene            complement(5769..>5999)
                     /locus_tag="PLC71_06015"
     CDS             complement(5769..>5999)
                     /locus_tag="PLC71_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit EIIBCA"
                     /protein_id="HRM02231.1"
                     /translation="AISASKKFGTNMYYAASIALIMLHPNLITLMNTAHDAGQTVKFL
                     KYIPVTYASYSYSVIPIILAVYSLRYCRTFCR"
BASE COUNT         1643 a         1335 c          936 g         2085 t
ORIGIN      
        1 atgatgtaag tgtcaaaagt ataaattgcg tttgtgcttg cacaaatatc caaaaagaga
       61 tccaccataa ttgatgaatc tcttttcgtt agaatgaatt gttatagatt atatgaataa
      121 tgatcgactt atttgtcttt caatactgcc tgagctgctg ctaatcttgc aattggtaca
      181 cggaatggtg agcaggatac atagtctaat ccggctctgt agcagaattc tacagatgat
      241 ggatctccac catgttctcc acagattcct aagtggatat ctggacgagt tgcttttcct
      301 ttcttagctg ccatctcgat cagctgtcca acacctttct ggtctaattt agcaaatgga
      361 tcagattcgt aaatcttgtt ctcatagtaa gactctaaga acttacctgc atcatcacga
      421 gagaatccaa atgtcatctg tgttaagtca tttgttccga aagagaagaa ttctgcttct
      481 tcagcaatct cgtctgccaa taatgctgca cgaggaatct caatcattgt accaactttg
      541 tacttaatgt cagattcata gtaagctttt actttctcag ctgtctgtac aacaacttct
      601 ttaacatatg ctaactcttt cttctctcct actaatggaa tcatgatctc aggaacgata
      661 tcatatcctt tttcctgttt tacttcgatc gctgcttcca taacggctct tgtctgcatc
      721 ttggcaatct ctggataagt aactgccaga cggcatccac gatgtcccat catagggtta
      781 aattcatgaa gagctgcacg tctcatattg atctcttcta ctgtaactcc catatctttg
      841 gcaagagctg tgatctcttc cattgtattt ggaaggaact catgaagagg tggatctaag
      901 aaacggattg tgaccggtct tccttccata acctcatata aatctttaaa gtctttcttc
      961 tgataaggaa ttaactgatt taatgcgatt tctcttgctt cttttgttgt agagaggatc
     1021 atacgacgga tctttggaat tctctctgcg tcaaagaaca tatgctctgt acggcaaagt
     1081 ccaataccct gtgctccgaa tttcactgca ttcaatgcat cggcaggtgt atctgcattt
     1141 gttcttacct gtaatgtacg gatttcatcg gcccatgtca tgatacgatc gaaattacca
     1201 ctgatacttg caggtactgt acggatctca cccagataaa tcttacctgt ggatccatct
     1261 aaggaaatat aatctccttc ctgtactgtc tttccaccta atgtgaaaga tttgccatct
     1321 tcagcaaact tgatatctcc gcatcctgct acacacgctg ttcccatacc acgtgctaca
     1381 acggctgcgt gagatgtcat accaccacga gctgttaaga taccttctgc tgcatgcatt
     1441 ccttcgatat cttctggtga tgtttctaaa cgaactaaga taactttcag tcctgcttca
     1501 tgatgcagtt tcgccatctc agcatcaaaa tatactttac cagcagcagc tcctggagat
     1561 gctggaagtg cttctccgat tggaagagcg gctttcaatg ctgcctggtc aaagtttgga
     1621 tgcagtaact gatctaatgc tttcgcatcg attcttgaaa ctgcttcttc ttttgtgatt
     1681 tttccttcat ctactaattc acaggcgata cggagtgcag ctggggctgt tctcttacca
     1741 tttcttgtct gtaagaagta cagttttcct tcctgaattg taaattccat atcctgcata
     1801 tcacgataat gttcttccag tgtatgagca atgctcataa actgttcata gcattctggt
     1861 aagtcttctg ccagtttagt aattggctgt ggtgtacgaa caccagctac tacgtcttca
     1921 ccctgtgcat tgatcaggta ttctccgtag atctgtttct cacctgtaga tgggtttctt
     1981 gtaaatgcca cacctgttcc tgatgtatct cccatgttac caaataccat tgtctgaacg
     2041 ttaacagctg ttccccagtc tcctggaatg tcgttcattc gacgataata gatagcacgt
     2101 gggttgtccc aagagcggaa taccgctttg attgcttcaa ataactggtc ctttggatcc
     2161 tgagggaatt cttcattcaa agctttgcga tagatacctt tgtatctctt gatcacttcc
     2221 ttcagatcat cggcatctaa gtctgtatca aacttcacac ctttctctgc tttgatgtca
     2281 tctaagacat cttcgaattt cgctttatcc acttctttta caacgtctga gaacatctgg
     2341 ataaaacgac gataagaatc atatgcaaaa cgaggattgc ctgtcttctt cgcaaatcct
     2401 tcgacagctt catcattcaa tcctaagtta aggatcgtat ccatcatacc cggcatggat
     2461 actcttgctc ctgatcttac tgatactaat agaggatctt caaggtctcc gaacttcttt
     2521 ccctgaatct cttccaagtc tttcattgct tcaattacct gtgatgctac ttcatcactg
     2581 atctttccac cgcagtcata atagtttgtg cacgcttctg ttgtaactgt gaatccctga
     2641 ggaattggta aattaagtcc tgtcatctct gctaagtttg caccttttcc tccaagaagg
     2701 ttacgcattt cggcatttcc ttcactaaat ttgtagaccc attttgttgc catgtcaata
     2761 cctcctaaaa ttaattatgt cacattcata tgtcattgaa cttattgtac cacaaatttc
     2821 agattttgca aaactttttt gtgtatttta cataattttt ttcatttttc tcgtttttca
     2881 ttataaaaac tgcacattta catgattttc gatgcaattt atgctgttat ttttgtctta
     2941 ttttaacgat ttttgtgttt tattttttgt atacaatata taaaaatccc agagtctttt
     3001 cactgaaaag atcctaggat ttcattttta tatcaaaatt attcagtttt ttaagaatgt
     3061 tttcttttta tagtctggtt tttgctgctt ctttttttct ttatccaaaa tttctaatta
     3121 tttattcttt aaacagattc tctccatcct gtttgatcac atcttgatac caataaaaac
     3181 tatctttctt atatcgatct aatgtagcgc cctcttcttc ttcgcgatct acatacacaa
     3241 atccatatcg tttttgatat ccattcagcc agcttaacaa atccgtaaaa gaccatgtac
     3301 aatatgcaag cacttcacag ccttcctcga tggcttttcc taattctttt aaatgttctc
     3361 ttagataatc aattcgatat tcatcatgaa tcttatgatc ttccgtcttc ttatcaaatg
     3421 ctccaagtcc attttccgaa atgatgattg gcagatcata acgacttgtg atctcacgac
     3481 aacaatagcg aagcccaact ggatcaatac tccaatccca gtctgttgtc atcaaatatg
     3541 gattgcctgg atttttataa attccaggaa ctccaagtac ctgcgcagaa cctttcactc
     3601 ctgttgtatt cattgtacca tatggtgtta ctccatccat cggattatac tcgcatacac
     3661 aactttgata ataattaact cccataaaat caatctctgc cgctgctgcc tttaaaattt
     3721 tctgatcgtc ttttgtaaca actggtgcaa atcctttttt tcttaaataa ttcatggctg
     3781 ctttaggata tcttccatat gcataaacat ccatccacca gaaattcttc agttcatcat
     3841 aatcatgttt tgacataaca ttttcaggtt tacaatcaag tgcataagat ggtgtataag
     3901 caaagcttgc accaatcttt cctgttccgc ccatctcgcg atatgccaaa acagcctttg
     3961 catgtgccat aaagacgtga tggttcactt gataaaatgt tttctgatcg tcaaactttc
     4021 ctggaggatg ctgtgctgtc agccatccaa gtgatgtaaa aatattttgt tcattaaatg
     4081 taatccagta ttttacctta cttccatatg ccttaaataa agtcttagca taatttacat
     4141 aatcctcaat gatctcctca gattcccatc ctccatagag atccactaat gcctgaggaa
     4201 gatcccaatg gtagattgtc accattggtt caatcccata ttttaaacat tcatcaataa
     4261 tattttcata aaactcaatt cctttgggat tgacctctcc tcttccttcc ggatagattc
     4321 ttgcccatga aacagaaaaa cgataagttt ttaatcccat ttccgccatc aacgcgatat
     4381 cttctttgta gcgatgataa tggtcaactg caacatctcc ggttgtcgct ttatatgttt
     4441 ttcctggaat ccttacaaat tgatcccagt ttgtgatccc ttttccatct tctctccagc
     4501 cgccttcaat ctgataggca gctgaagcac ttccccacaa aaatccttct ggaaattttg
     4561 taactttcat ttcttccata aatgttacgc ttcctttctg ctactgtaca cttaataatt
     4621 tacttttctt tgttactact ttatcttcac atgctttaac ttttgcaaat tgttctgtat
     4681 tggaaataat gatcggtgtt gttacatcgt atccatctgc tttaatcttg tcaatatcaa
     4741 attctagtaa gatatcccct tttttcattg tatctccaga ttttttcttt ggagtaaaat
     4801 attttccttt taaatttact gtatcgattc ctacatgtac caaaagttct actccatctt
     4861 cacttactaa tcctaatgca tgtcctgttt caaaaacagc atctactttt ccgtcaaatg
     4921 gtgcatagac aactcctttt tcaggaatga tcgctgctcc ttttcctaag atttcttgcg
     4981 aaaatgttgg atctttcact tctgttaaag gaatcacttt cccttcaagt ggacttgtaa
     5041 tgctgatctc attatctaat aactgtgcat tagattcttc tacaaaatct aaatcatcat
     5101 cttcatctac aatatcttca aagccaataa tcagtgttgc aacaaatgta acaactactg
     5161 tgaccaaaat tgtgagtgct gcaaataata atgtctgtgg ttttcctttt tctacatact
     5221 gaacaatcgt tacgattgct ggtgttgcaa ttccaaaaca tttcatttgg aaaaatccac
     5281 cgactaatcc accaacagca ccaccaatac atgctcctaa cattggtcgt tttaaacgaa
     5341 gtgttacacc ataaagtgca ggttctgtga taccagcaaa taatgctgaa aacgcagaag
     5401 atcctgcgat ctgtttaaag tctttgtttt tgcttcgaag tgcaactgca agggaggctg
     5461 caccctgtgc catattagaa cataattctc cgatacagat aaagttctca tatcctgttg
     5521 ttgcgatcat ccctaattta atcggtgtaa atgcatgatg catacctgcc ataacaacga
     5581 atggataaac gccagccaca agaccaatgg caataaatcc taatttatca tgaattgcat
     5641 aaactactgt tgataatcca ttaccgcaaa tagctcctaa aggtccaaga atgatcaatg
     5701 cgatcggtgc ttcaattaaa acaactaaca ttggttttaa gaagttcttt gtgacaactg
     5761 gcgtaatttt atctacaaaa cgttctacaa tatcttagag aatatactgc caaaatgatt
     5821 gggataaccg aataagaata ggatgcataa gttaccggaa tatatttcaa aaatttcaca
     5881 gtctgtcctg catcatgtgc tgtattcatc agtgtgatca agtttggatg taacatgatc
     5941 agtgcaatac ttgctgcata atacatattt gttccaaact ttttggatgc tgaaattgc
//
LOCUS       DAOMUE010000107         5964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3594_length_5964_cov_2.580132, whole genome shotgun sequence.
ACCESSION   DAOMUE010000107 DAOMUE010000000
VERSION     DAOMUE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3594_length_5964_cov_2.580132"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLC71_06020"
     CDS             <1..304
                     /locus_tag="PLC71_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HRM02232.1"
                     /translation="QDGKVDVKEVLKQLRNMEIDSVLVEGGGILNESLIKNNCVHKVY
                     AYIAPKLFGGEKAKTPVEGKGIERIQEALVFDELKATPLGNDILLEGKVRSCLPES"
     gene            286..945
                     /gene="ribE"
                     /locus_tag="PLC71_06025"
     CDS             286..945
                     /gene="ribE"
                     /locus_tag="PLC71_06025"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRM02233.1"
                     /translation="MFTGIVEELGTVISLKRGSKAAVLTIKANVIFEDLKLGDSVAVN
                     GVCLTATHISGNTFQADVMNETFNRSSLGSLKKGSVVNLERAMAANGRFGGHMVAGHV
                     DDTGELVSVKQDDNAVWFTIKAPENIMKYCIEKGSIALDGISLTIAKLSDDTISVSMI
                     PHTIKNTNFGYKKPGDKINLENDMVGKYIDKLLHFDQQETNDKHTNSTLTMEMLRNAG
                     F"
     gene            1002..1571
                     /gene="ribA"
                     /locus_tag="PLC71_06030"
                     /pseudo
     CDS             1002..1571
                     /gene="ribA"
                     /locus_tag="PLC71_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015513956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
     gene            1653..2120
                     /gene="ribE"
                     /locus_tag="PLC71_06035"
     CDS             1653..2120
                     /gene="ribE"
                     /locus_tag="PLC71_06035"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRM02234.1"
                     /translation="MRVLEGKLVASGLKVGIVAARFNEFIVSKLVSGALDGLKRHDVK
                     EEDIDIAWVPGAFEIPLIADKMAKSKKYDAVICLGTVIRGATSHYDYVCNEVSKGIAQ
                     VSLNTGIPVLFGVVTTENIEQAIERAGTKAGNKGYDCALSAIEMVQLIKEVEA"
     gene            2152..3710
                     /locus_tag="PLC71_06040"
                     /pseudo
     CDS             2152..3710
                     /locus_tag="PLC71_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202904.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            3844..4572
                     /locus_tag="PLC71_06045"
     CDS             3844..4572
                     /locus_tag="PLC71_06045"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HRM02235.1"
                     /translation="MDNEKIEKLQQMIDESNHIVFFGGAGVSTESHIPDFRSTDGLYH
                     QTYKYPPEVMVSHTFYTQHTEEFYDFYKNKMIFLDAKPNKAHLKLAEMEQAGKLSAIV
                     TQNIDGLHQAAGSKNVFELHGSVHRNYCQKCGKFFDVNYVVNAKGVPYCDECGGIIKP
                     DVVLYEEGLDSQTINGAVRAISKADMLIIGGTSLVVYPAAGMIDYFKGKYLAVLNRDA
                     TSRDKQADLCITDPIGEVLGRIRV"
     gene            4575..4943
                     /locus_tag="PLC71_06050"
     CDS             4575..4943
                     /locus_tag="PLC71_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HRM02236.1"
                     /translation="MKKKDIILIAAVLAISLISLAAIKMTQKDGKEVIVTVDGKEVYK
                     TSIKKDQIYQIPEENGTNVMQIKDGKVTMKEADCKDQICADHKAIEKSGETIVCLPHK
                     VVIEIRSEDGKEQELDGVTQ"
     gene            4984..5481
                     /locus_tag="PLC71_06055"
     CDS             4984..5481
                     /locus_tag="PLC71_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HRM02237.1"
                     /translation="MSTKRLATDAMFLTLALVVSYIEVLIPVPVGIPGVKLGLANGVI
                     MVLLFFTTWTRTLEISVIRVVLAGFLFGNPMTIAYSLAGGILSLVIMGLLKKIDGFSP
                     IGISVGGGVAHNIGQLSVAVFLMENTKIYYYAPVLLITGTIAGIIIGVLSGVLVKKIP
                     PQLFR"
     gene            5567..>5964
                     /locus_tag="PLC71_06060"
     CDS             5567..>5964
                     /locus_tag="PLC71_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HRM02238.1"
                     /translation="MRTKLNEIIKNGHLGMIAVFFIVYMITFSYIEDRDVHHYIIHTT
                     IDDQIPFCEYFIVPYLLWFLFVAVTVFYFMFFNKNQWKEYYQLIITLGTGMTLFLIIS
                     LVFPNGQDLRPVLTGDSIFIKAVQMIYHTDT"
BASE COUNT         2147 a          967 c         1372 g         1478 t
ORIGIN      
        1 gcaagatgga aaggttgatg ttaaagaagt tctaaaacag ttaagaaata tggaaatcga
       61 cagtgtttta gttgagggcg gcggaatctt gaatgaatcc ttgatcaaaa acaactgtgt
      121 tcacaaagtt tacgcctata ttgctccaaa attatttgga ggagaaaagg ccaaaacacc
      181 tgtagaaggc aaagggatcg aaaggatcca agaagctttg gtatttgatg aattaaaggc
      241 aacaccactt ggaaatgaca tattactgga agggaaggtg agatcatgtt taccggaatc
      301 gtagaagaat taggaacagt catatccctg aagcgaggaa gcaaagcagc agtccttacg
      361 atcaaagcaa atgttatttt tgaagatctt aaattgggag acagcgtagc agttaatggg
      421 gtctgcctga cagcgactca cattagcggc aatacatttc aggcagacgt gatgaatgag
      481 acattcaacc gaagcagtct tggaagttta aaaaagggat ctgtcgtcaa cttagagcga
      541 gcgatggcag ccaatggaag gttcggaggt catatggtag caggccatgt ggacgataca
      601 ggggaacttg ttagtgtaaa acaagatgat aatgctgtat ggtttacgat caaagcccca
      661 gaaaatatca tgaaatactg tattgaaaaa ggatcgatcg cactcgatgg aatcagcctg
      721 acgatcgcta agttaagcga tgataccatt tccgtatcca tgatcccaca taccataaaa
      781 aataccaatt ttggatataa aaaaccagga gataagatca atctggagaa tgacatggtt
      841 gggaaatata tagataaact gctgcatttt gatcagcagg aaacaaacga caaacataca
      901 aacagcacct taacaatgga aatgttaaga aatgctggat tttaataaaa aacgaggagg
      961 gaaaagacaa gatgagcgat gtagtatgcg aagtaatcgc acagcttcca acaaaatatg
     1021 gaacatttga ggcacatggg tatgtagacc agagaagtgg ggaacatcat gtagcactgg
     1081 tcaaaggaga tatcgcagac ggagaaccag ttttatgccg tgtgcattct gagtgcctga
     1141 ctggggatac ttttggatct ctacgctgtg attgtggaga acagcgaagc cgtgcaatgc
     1201 gtcagatcga gaaagaagga cgaggggttt tcttatatat gcgtcaagaa ggacgaggaa
     1261 tcggattgat caataaactg aaagcttatg tattacagga acagggaatg gatacagtcg
     1321 aggccaattt agctcttgga tttaaagatg atgaacgaga ttacagtgca ggagccatga
     1381 tcttacagga tttaggaatc aaaaaactac gactgatgac aaacaatcca accaaaattg
     1441 acggattaaa tgaatatgga ctcgaaatcg tagaacgagt accgatccag atgccgatca
     1501 atccatttga taaatattat atgcagacaa agcaaaagaa aatgggacac atgacaacat
     1561 acgaagttta aattaacaga aaaattaaaa aaattaaggt cccagggacc ttaagattaa
     1621 cgaaattaac gaagaaaaaa ggagtaatta aaatgagagt attagaaggt aaattagtag
     1681 caagcggatt aaaagtagga atcgtagcag ccagatttaa tgaatttatc gtatccaaat
     1741 tagtatccgg agctttagac ggattgaaaa gacatgatgt gaaagaggaa gatatcgata
     1801 tcgcatgggt accaggagct tttgagattc cactgatcgc agacaagatg gcaaaaagca
     1861 aaaaatacga tgcagtgatc tgtcttggaa cagtgatccg tggagcaaca agccattatg
     1921 attatgtatg taacgaagta tccaaaggaa tcgcacaggt atcattaaat acaggaattc
     1981 cagtcttatt cggagttgtt acaacagaaa atatcgagca ggcgattgaa cgtgctggaa
     2041 caaaagcagg aaacaaagga tatgactgtg cattatctgc aatcgaaatg gtacagctga
     2101 tcaaagaagt agaagcataa aaagaatcag aaattaaagc aggaagttag tatggaacaa
     2161 tattatttag gatttgacgc aggaacacaa agtgtaaaaa cagcagttta tgatattgat
     2221 atgaacctga tcgtgcagga taccaatgca acaatcttaa attatccaca tccgggatgg
     2281 gtggaaatgg atgcagacca gtatcttcat gcaacggtta cagggatcag aaactgcgtt
     2341 acaaagatga aagaaaagaa tctggatcca gacaacatcc gctctatttt tggatcatct
     2401 gcgggattgt cggtgttgat aaggactgcc atgcgatcac accttacatc aattatctgg
     2461 attccagaac aaaagaagat gttgaggaac tagcatccca tcattatgag atctgggcaa
     2521 aagagactgg aaatcctcag ccaaactgta tgtttccagc gatgtttgca cgttggatga
     2581 tgaacaattg tgggggcttt aaagaaaaag gcagaaaatt catgcacaat gccccatata
     2641 tcctggccaa tcttgcagga ctgtctgcag aagatgcatt tattgactgg ggaacaatgt
     2701 ctggatgggg actaggatac cgtgtaacag agaaaaaatg gtccaaggaa caattagaga
     2761 tccttggaat tccaatggaa atgatgccaa agattcaaaa accatgggat atcattggaa
     2821 cattatcaga gacatttgca aaagaaaccg gattgaaacc aggaatccca atctgtgcgg
     2881 gagccggaga tacgatgcag tccatgatcg gatgcggagt gatcaaacca gatcaggcag
     2941 ccgacgtagc aggaacatgt gcgatgtttt gtatcgcaac cgatgggatc aatgaagaat
     3001 taagcaaacc agaaaatgaa ctgatcttta acagcggaac tttagaaaat acatattttt
     3061 attggggatt cataagaacc ggtggattag cacttcgttg gtatcgagat aatgtatgca
     3121 acgaagcagg aaatggagca tattttgatg aattaaatga gaaagcacaa aatgtgccat
     3181 gcggatccaa aggagtcctg ttccttccat atttaacagg aggatttgga gaaacatcca
     3241 atgcaagtgc ctgtttcctg aatatgacga tggacacaga tcagggagta cagtggcgtg
     3301 cagttttaga agcgatcggt tacgattata tgagtgtgac agatatttat aaaaaagcag
     3361 gcgtgccact tgaatttatg acgatcacag aaggtggaag caattcagaa gtctggaatc
     3421 agatcaaagc agacatgtta ggatgtaaga gtgccacatt agaaaatgca caaggagcag
     3481 ttcttacaga tgcagtgatc gcagcatatg cggtaggaga tctggaagat ccaagtcagg
     3541 tatttgaaaa aacgagaaag atcaagaaaa tttatgaacc tgatctaata agaacaaaat
     3601 actatcgcag tatttatgaa caacaaagaa aactaattaa agacgatatg gcagcagttt
     3661 ttgagacttt acaccagatc agtaagattc aagaacaaag caatgattaa aaaaatgaaa
     3721 aggcttcttt acaagaggcc tttttttctt gtacaataaa agataaagta tataaaaata
     3781 agttataagc aactaacttt gtagatgatt tttgatcaat tagatttcaa ggaggaaaaa
     3841 gtcatggata acgaaaagat tgaaaaatta caacagatga tcgatgaaag taatcatatc
     3901 gtattctttg gtggcgcagg tgtttcaaca gagagtcata ttccagactt tcgaagcacg
     3961 gatggtttgt atcaccagac ttataaatat ccacccgagg ttatggtaag ccatacattt
     4021 tatacacagc atacggaaga attttatgat ttttataaga ataagatgat cttcttggat
     4081 gcaaaaccga ataaggcaca tttaaaactg gcagaaatgg aacaggcggg aaaactgtct
     4141 gcgatcgtta cgcaaaatat cgacgggctt catcaggcag caggaagtaa aaatgtcttt
     4201 gaactgcacg gcagtgttca cagaaactat tgccagaaat gcggcaagtt cttcgatgtg
     4261 aattatgtcg tgaatgccaa aggagtccca tattgtgacg aatgtggggg aatcatcaaa
     4321 ccagatgttg tattatatga agaaggttta gattctcaga caatcaacgg agcagtcaga
     4381 gccatttcca aggcagatat gctgatcatt ggaggaacat cactggttgt ctacccagcc
     4441 gctggaatga tcgattattt taaaggaaaa tatcttgcag tactgaatcg tgatgcgaca
     4501 agcagagata aacaggcaga tttgtgcatt acagacccaa tcggagaagt actgggaaga
     4561 atcagggtat aaagatgaag aaaaaagata taatcttgat cgcagcagta ctggcaattt
     4621 cactgattag tttggcagcg atcaagatga cacaaaaaga cggaaaagaa gtgatcgtga
     4681 cagttgacgg aaaagaagtg tacaaaacat ccatcaagaa agatcagatc tatcaaattc
     4741 cagaggaaaa tggcacgaac gtcatgcaga tcaaagatgg aaaagttaca atgaaagaag
     4801 cagactgtaa agaccagatc tgtgcagacc ataaagcaat cgagaaatca ggagaaacga
     4861 tcgtatgtct gccgcataaa gtagtgatcg agatccggtc agaagatggc aaagaacagg
     4921 aattagacgg ggtaacacaa taaacatcag atgagataga aaacaacagg agaaataaaa
     4981 tatatgtcaa caaaaagatt agcgacagat gcgatgtttt taacattggc acttgttgta
     5041 tcctatatag aagtattgat cccggtacca gttggaattc ccggagtgaa attaggactt
     5101 gccaatggag tgatcatggt tttgttattc tttacaacat ggacaagaac acttgagatc
     5161 tcagtgatcc gagttgtact ggcaggattc ttgtttggaa atccaatgac gatcgcctac
     5221 agtctggcag gaggaatctt aagtctggtc atcatgggac ttctaaagaa gattgatgga
     5281 ttctcaccca ttggaatcag cgttggaggc ggagtagcgc ataatatcgg gcagctcagt
     5341 gtcgctgttt tcttaatgga gaatacaaag atttattatt atgcaccagt attactgatc
     5401 acaggaacga tcgcagggat catcatcgga gtgttaagtg gagtgctagt taagaagatc
     5461 ccgccacaac tatttcgata aaagatttga aatttctaca aaatcgtggt actactatat
     5521 gtatacactt tctaacaggc aatgattttg aagaaaggag tcacaattga gaacaaaatt
     5581 aaatgagatc ataaaaaatg ggcatcttgg tatgatcgca gtatttttta tagtttatat
     5641 gatcacattt tcctacattg aagatcgaga tgtgcatcat tacatcatac atacaacgat
     5701 agatgatcag ataccattct gtgaatattt catcgtacca tatctattat ggttcttatt
     5761 tgtagcagtt acagtttttt attttatgtt ttttaataag aatcaatgga aagaatatta
     5821 tcagctgatc ataacacttg gaacaggcat gaccctgttt ttgatcatct cattagtatt
     5881 tccgaatgga caggatctaa gaccagttct tactggagac agcattttta tcaaagcagt
     5941 acagatgatc tatcacacgg atac
//
LOCUS       DAOMUE010000108         5939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3616_length_5939_cov_2.392930, whole genome shotgun sequence.
ACCESSION   DAOMUE010000108 DAOMUE010000000
VERSION     DAOMUE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3616_length_5939_cov_2.392930"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLC71_06065"
     CDS             complement(<1..333)
                     /locus_tag="PLC71_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM02239.1"
                     /translation="MDKLKKMTSYRLFWPIVCLALVLLFNLIKSPSFFEISIKNGVLY
                     GYLVDIFNRSSELIILAVGMTLCVASSAGTDISVGAIMALSGAVSVYLLGSGETYAIP
                     WIAAILIGL"
     gene            complement(336..1853)
                     /locus_tag="PLC71_06070"
     CDS             complement(336..1853)
                     /locus_tag="PLC71_06070"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HRM02240.1"
                     /translation="MSDEKKTVLSMRHINKTFPGVKALSDAQLTLREGEIHALMGENG
                     AGKSTLIKVLTGVEEFETGEIIMDGKSIINTSPQEAGNNGISTVYQEVNLCPNLTVAE
                     NIFIGREPMKMGRIDWKTMNTQAQKILDNLELEIDATQELENYSVAIQQMVAIARAVD
                     IQSKVLILDEPTSSLDEGEVEKLFKVMNMLKKRGTAIVFVTHFLEQVYAVCDRITVLR
                     NGHFVGEYKTEELPRFKLVATMMGKEFDDLADIKGSGTSSKKQSDEVVIEAKGLYHKG
                     TIKPFDIKIHKGEVVGLSGLLGSGRSELVRAIYGADKPDGGELYVKGEKLDVKAPIDA
                     MHQGMAYCPEDRKVEGIIADLSVRENMILALQAKRGMFKLMSRKEQEELTDKYIKMLQ
                     VKTASRETPIKSLSGGNQQKVILGRWLMTEPDFLILDEPTRGIDVGTKTEIQKLVVKL
                     ANEGMSVVFISSEVEEMLRTVTHMGILRDGEKVGELEESELSQENVMKAIAGGDK"
     gene            complement(2032..>2173)
                     /locus_tag="PLC71_06075"
     CDS             complement(2032..>2173)
                     /locus_tag="PLC71_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HRM02241.1"
                     /translation="PLHGPRVAELAKKIMKGEKVDKIQYVDEQVFTKDNTTKEVINKR
                     AY"
     assembly_gap    2174..2205
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2206..3051)
                     /locus_tag="PLC71_06080"
     CDS             complement(<2206..3051)
                     /locus_tag="PLC71_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM02242.1"
                     /translation="MRRSIKKVAAGLLATMMIGTMLTGCGGGNKKDSGSSKKETSEIN
                     IGFSQVGAESDWRVANTKSMKSALTAKNGFKLSFADAQQKQENQTKAVREFITQGVDV
                     IAIAPVTETGWETVLKEAKKADIPVITVDRQIKTSDDSLITAWVGSDFKKEGVKAAEW
                     LIKEKGKEKGDVNIAVLQGTIGSSAEIGRTKGFKEGIKDQKNFKIIASQTGEFTQAKG
                     QEVMESFLKQNKDIDVVVAQNDNMAFGAIDALKAAGKTPGKDVTIISFDAIKAALKKV
                     QSGEIN"
     gene            complement(3239..4705)
                     /locus_tag="PLC71_06085"
     CDS             complement(3239..4705)
                     /locus_tag="PLC71_06085"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRM02243.1"
                     /translation="MKKYVRESIARFRQFSIEKKLKWLRMVVIIPALVGIITLLVIMI
                     NFNESYNEAVKNVSVASKFNFSFSEDMDYKMYRIVIGAESFDAMKPYEEIKEAKELVK
                     ELNKNAVTDESKLRTRQIGKLLDNLKISIEEIEHSDLKNDYMENNQRLRLNVNVFTEI
                     IKEKVSEYIYYEIGNMESMRVKLEKEIMHTITITLIAVLFLSLITWKLSKLISQSISY
                     PVRQLCEMTKEVAQGNFDVHGPQNTTEELQILTNNFEHMAQKVERLIEDVKVEQQNLR
                     KKELQLLQEQINPHFLYNTLDTIMWLAIDHQDDKVVEMVAALSGFFRTSLSHGEDKIT
                     IREELEHVENYLKIQQLRYGDIMEYSIDVPDEIKKNDIVKITLQPLVENALYHGLKKQ
                     REKGMIKISSVDDENNIFLIVEDNGVGMQPEQVVQINKEMYEDIWVNRTTGFGIANVN
                     RRIKLYYGEEYGLILESEPDIGTKVIVVVPKTRQAEKK"
     gene            complement(4705..>5939)
                     /locus_tag="PLC71_06090"
     CDS             complement(4705..>5939)
                     /locus_tag="PLC71_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRM02244.1"
                     /translation="EEDIKKLQKNIKTQMKNMRYQFFGNLIRGKISVSKLMEQGNELG
                     IDLMAPAYNFMLFKIFSKNENDTEAVYDLRTKEDAIVEEVSSQFENMIVFHRVTEGYV
                     LMIKAQNTEETIQVAKDYVAALTKRMKKEKELQWFAGIGHAVERLHNLSESYDSASKA
                     FAYQYQSSGNEAVFFDKLDNEKIERAEDLQQVDFTKVNSESLEEFLKNGKEEAVHLFV
                     EDYTKTLGKSNMDSFMFCQYMLINIQVGVMKFVEKMGVEKANIDRTFKDYKQQIAAVS
                     TGEKAAEYIEELIIQAIRMRNERLGKKHSSLITEAKEFIVKNYQEETLSLSDVADQVG
                     LSSSHFSTTFKQETGKSFVEFLTSVRMDKAKEMLCFTDMKASQISYEVGYKDPHYFSY
                     LFKKTQGCTPSEYRSRRM"
BASE COUNT         1506 a         1302 c          827 g         2272 t
ORIGIN      
        1 aagcccaatc aggattgctg cgatccaagg aattgcatat gtttctccac ttccaagaag
       61 ataaacagat acagctcctg ataatgccat gatcgctccg acagagatat cagttcctgc
      121 actggatgct acacaaagtg tcataccaac tgctaagatg atcaattcac tggatcggtt
      181 gaaaatatcg accagatatc catataacac accattttta attgagattt caaaaaatga
      241 tggggatttt ataaggttaa ataataatac aagtgccaga catacaattg gccagaagag
      301 gcgatatgat gtcatctttt ttaatttatc cataattatt tgtcacctcc tgcgattgct
      361 ttcattacat tttcctgtga taattcactt tcttccaatt ctccgacttt ctcaccatct
      421 cgtaagattc ccatgtgagt cacggtacga agcatttctt ctacttcgga tgaaataaat
      481 acaacagaca ttccttcatt tgcaagtttt acgactaatt tctgaatctc tgtctttgtt
      541 ccaacatcaa tacctctggt tggttcatca aggatcagga aatctggttc tgtcatcagc
      601 catcttccga ggattacttt ctgctgatta ccaccactta atgatttgat cggtgtttct
      661 ctgcttgctg tcttaacctg cagcatctta atatacttat ctgtcaactc ttcctgttct
      721 tttctgctca ttagtttgaa cattcctcgt tttgcctgaa gtgccaagat catgttttct
      781 cttacagaaa gatctgcaat gattccttct actttacgat cttccggaca atatgccatt
      841 ccttgatgca ttgcatcaat tggtgctttg acatctaatt tttctccttt gacatataat
      901 tctcctccat ctggtttatc tgctccataa atcgctctta caagttcgga acgtccagat
      961 cctaataatc cagataatcc aacaacttct cctttatgga tcttaatgtc aaatggtttg
     1021 atcgttcctt tatgataaag accttttgct tcgatcacaa cttcgtcgct ttgtttcttt
     1081 gatgatgttc cacttccttt gatgtctgcc agatcatcaa attcttttcc catcattgtt
     1141 gcaactaatt taaatcttgg aagctcttct gtcttatatt cgcctacaaa atgtccattt
     1201 ctaagtactg tgatcctgtc acagactgca tagacctgtt ccaaaaagtg tgttacgaat
     1261 acgatcgctg ttccgcgttt ctttagcata ttcataacct taaataattt ctcaacttct
     1321 ccttcatcaa gacttgatgt tggttcatca aggatcaata cttttgactg aatgtcaact
     1381 gctcttgcga tcgccaccat ctgctgaatc gctacggaat agttttccaa ttcctgtgtg
     1441 gcatcaattt ctaattccag attatcaagg atcttctgtg cctgtgtatt catcgtcttc
     1501 cagtcgatcc tccccatttt cattggttct cttccgataa agatattttc tgctaccgtt
     1561 agattcggac acaggtttac ttcctgataa acggtgctga ttccattgtt tcccgcttct
     1621 tgaggggatg tgttaataat actttttcca tccatgatga tctcacctgt ctcaaattcc
     1681 tcaactcctg taagaacttt gatcagtgta gattttcctg caccattttc tcccattaag
     1741 gcatgaatct ctccttctct caatgtaagc tgtgcatcgg acagtgcttt cactcctggg
     1801 aatgttttat taatatgtcg catggataga actgtcttct tttcatcact catgacaatt
     1861 ctcctttctt ttcatgattt ttgagccgat tttaatgaaa atgccctgat ttttggcata
     1921 aaaattacag gataagtcag attgtcctga cccagagcct gcaatcttta tttgtagtgc
     1981 gattgttaat agttacatgt gtgtacttaa ggggattttc aacaatgttt attaatatgc
     2041 acgtttgtta ataacttctt ttgtagtatt atcttttgta aatacctgct catctacata
     2101 ctggatctta tctacttttt cacctttcat gatcttctta gctaattcag ctacacgagg
     2161 tccatgaagt gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnattga tctctccaga
     2221 ctgaactttc tttaatgctg ctttgatcgc atcgaaagaa atgatcgtta cgtctttacc
     2281 aggtgttttt cctgctgctt ttaatgcatc gattgctccg aatgccatgt tatcattctg
     2341 tgctactaca acatcaatgt ctttgttctg ttttaagaaa gattccataa cttcctgacc
     2401 ttttgcctgt gtaaattctc ctgtctgaga agcaatgatc ttgaagtttt tctgatcttt
     2461 gattccttct ttaaatcctt ttgtacgtcc gatttctgca gaagaaccaa tcgttccctg
     2521 taatacagcg atgtttacgt cacctttttc ttttcccttt tctttaatta accattcagc
     2581 cgctttcaca ccttcttttt tgaagtctga tccaacccat gctgtaatca gtgaatcatc
     2641 agaagttttg atctgacggt caactgtgat aactgggatg tcagctttct ttgcttcttt
     2701 taaaactgtt tcccaacctg tttctgttac aggtgcgatt gcgatcacat caacaccctg
     2761 tgtgataaat tctctgaccg cttttgtctg attttcctgt ttctgctgag catctgcaaa
     2821 tgataattta aatccgttct ttgctgttaa tgcagatttc atagactttg tattcgctac
     2881 acgccaatca ctctcagctc caacctgtga aaatccgatg ttgatctctg aagtttcctt
     2941 ctttgaagaa ccactgtctt tcttgtttcc tccaccacaa ccagtaagca ttgttccgat
     3001 catcattgtt gcaagtaaac ctgcagccac cttcttaata cttcttctca ttatgatttc
     3061 ctcctttttc ttttttgatt ttttcgtttt ccttaattac aagttatatt ttacttgaaa
     3121 ttgtatttct ttcaatgcaa tttcttttgc attcggtgga ttattttatc aaatttttat
     3181 cttgtctgtt taaagtttga ttttttttga taatggtttg aatttttttg atttttgatt
     3241 attttttctc tgcttgtcta gtcttcggta caactacgat caccttagtt ccaatatctg
     3301 gttcactttc aaggatcaac ccatattctt ctccataata taatttaatc cgccgattta
     3361 catttgcaat tccaaaccct gttgtcctgt ttacccatat atcttcatac atttctttat
     3421 tgatctgaac tacctgctca ggctgcatac caactccatt atcttcaacg atcaggaata
     3481 tattattttc atcatctaca ctggaaatct ttatcattcc tttttctctt tgttttttca
     3541 atccatgata taatgcattt tctactaatg gctgtaaagt gatctttaca atatcatttt
     3601 tcttgatctc atctgggaca tcaatagaat attccataat atccccgtat cttaattgtt
     3661 gtatctttaa ataattttca acatgctcta attcttccct gatcgttatc ttatcttctc
     3721 cgtgacttaa acttgtacgg aaaaagccag acaaggcagc taccatttct accactttgt
     3781 catcctgatg atcaatggca agccacatga tcgtgtccaa tgtattataa aggaaatgtg
     3841 gattaatctg ctcttgtagt aactgcagct ctttttttcg aagattttgt tgttctactt
     3901 ttacatcttc gatcaatctc tctacctttt gtgccatatg ttcgaaatta tttgttaaga
     3961 tctgcagctc ttctgttgta ttttgaggtc catgtacatc aaaatttccc tgcgccactt
     4021 cctttgtcat ctcacataac tgtctgactg gataactaat gctttgcgaa atcaatttcg
     4081 ataatttcca tgttatcaga gacaaaaaca gtacagcgat caatgtgatc gtgatcgtat
     4141 gcatgatttc tttttccagt tttacacgca tactctccat attcccaatc tcatagtaaa
     4201 tgtattcgga tactttttct ttaatgatct ctgtaaatac attcacattt aatcttaatc
     4261 tctgattatt ttccatataa tcgtttttta gatctgagtg ttcaatttct tcaatggaaa
     4321 tcttcagatt atccaacagt tttcctatct gtctcgttct taatttactt tcatctgtta
     4381 cagcattttt attcagttcc tttaccagct cctttgcttc cttaatctcc tcatatggtt
     4441 tcatcgcatc aaaactctct gcaccgataa caatacgata cattttataa tccatatctt
     4501 ctgaaaatga gaaattaaac ttgctggcta ctgatacgtt ttttactgcc tcattatagc
     4561 tttcattaaa atttatcatg ataacaagaa gcgtgataat ccccactaac gcaggaatta
     4621 tcacgaccat ccttaaccat tttagttttt tttctattga gaactgccga aatcgagcaa
     4681 tagattctct cacatatttt ttcattacat cctccttgat ctatattcac ttggagtaca
     4741 tccttgcgtt tttttaaaca ggtagctaaa gtaatgtgga tccttatatc ccacttcata
     4801 gctgatctgt gatgccttca tatctgtaaa acaaagcatt tcttttgctt tatccattct
     4861 gactgatgtc aaaaattcca caaaagattt ccctgtttct tgtttaaacg ttgtactaaa
     4921 atgactgctg cttaatccaa cctgatctgc aacatcactc aacgataatg tttcttcttg
     4981 ataatttttc acaataaatt cttttgcctc cgttatcaat gaagaatgtt tcttccctaa
     5041 tctttcattt cgcattctga tcgcttgtat gataagttct tcgatatact ccgctgcttt
     5101 ctctcctgtt gaaactgcag cgatctgttg tttgtaatcc ttaaacgtgc gatcgatatt
     5161 tgctttctca actcccattt tctcaacgaa tttcataaca cctacctgta tattgatcag
     5221 catatactga caaaacataa aggaatccat attagatttt cctaatgtct tggtataatc
     5281 ctctacaaat aaatgtacag cttcttcttt tccatttttc aaaaactctt ccagcgattc
     5341 tgaatttact ttggtaaaat ctacttgctg cagatcttct gctctttcta tcttttcatt
     5401 atctaattta tcaaaaaaca ccgcttcatt cccagaagac tgatactggt atgcaaaagc
     5461 cttgcttgca ctgtcgtaag attccgaaag gttatgtaat cgttccactg catgtccaat
     5521 ccctgcaaac cattgtagtt ctttctcttt tttcatacgc tttgtcaatg ctgcaacata
     5581 atcttttgcc acttgtatgg tttcctctgt attctgtgcc ttgatcatta aaacatagcc
     5641 ctctgttaca cggtgaaaaa caatcatatt ttcaaactgg ctgcttactt cttctacgat
     5701 cgcatcttct ttggtacgaa gatcatatac tgcctctgta tcattctcat tctttgaaaa
     5761 tattttaaat aacataaaat tgtatgccgg tgccataaga tctattccaa gttcattccc
     5821 ttgttccatt aatttagata cagatatctt tccgcggatc agattcccga aaaattgata
     5881 tctcatattt ttcatttgtg tttttatatt tttttgtaat ttttttatat cttcttcct
//
LOCUS       DAOMUE010000109         5918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3640_length_5918_cov_2.334300, whole genome shotgun sequence.
ACCESSION   DAOMUE010000109 DAOMUE010000000
VERSION     DAOMUE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3640_length_5918_cov_2.334300"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLC71_06095"
     CDS             complement(<1..848)
                     /locus_tag="PLC71_06095"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HRM02245.1"
                     /translation="MAAIIGSPTRYIQGKGEMKNLCSYADKFGKNLFILTSASGRKRV
                     EPAINEGNKDSNLVYDVFNGECCMTEINRIIKIVEEAGSDVIIGIGGGKIHDTSKAVA
                     YYTKKPVIIVPTIASTDAPCSALSVIYTDEGVFEKYLFLPSSPDMVMVDTDIVCKAPV
                     RLLISGMGDALATYFEARACKRSDASNCVGGKCTLAAMNLAQLCYDTLMENGVQAMIA
                     AKEGICTKAVENVIEANTYLSGIGFESGGLAGAHAIHNGFTAIPETHKMYHGEKVAFG
                     TLVQLVL"
     gene            1146..2444
                     /locus_tag="PLC71_06100"
     CDS             1146..2444
                     /locus_tag="PLC71_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HRM02246.1"
                     /translation="MMIKTLFLLSFLFFIYSFIGWILEVVQSAFHQKRLVNRGFINSP
                     LCISYGIGAIVITINTHQMTGLWIFAAAMIDATVIEWFGGHFIEHFYHERWWDYSKNK
                     WNLDGYICLSHSVFWGLLGYIGVKFVNPLLFKFYHLIPPFIRHLIIFILLAVLIIDIL
                     ATTIVVFGKNIDKRRWESADAYLTKISVKLSSLITSYVDRRVERAYPQRKLKLPTIPK
                     TGVFAQGCGFYKVFLLFSIGSLLGDIIETIFCRLKMGVWMSRSSLVWGPFSIVWGFAF
                     AGVTLLLYRYKDRSDSFLFLTGTFLGGAYEYLCSVLSEIVFGKVFWDYSKMPFNLNGR
                     INLLYCFFWGIATVVWFKRIYPFLSNLIEKLPIAFGTVFTWIIVVFMVLNMFMSLSAL
                     IRYDQRNKKIPASNFFERYLDTHYNDQKMKLIYPKAKKVH"
     gene            complement(2545..3300)
                     /gene="dapB"
                     /locus_tag="PLC71_06105"
     CDS             complement(2545..3300)
                     /gene="dapB"
                     /locus_tag="PLC71_06105"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRM02247.1"
                     /translation="MVKIMMHGCNGVMGQVISKIVEETEGAVMACGVDRVDDGHNDYP
                     VFTDINACDVEVDAIIDFSAAPAVDGLLDFAVERQIPVVLCTTGLSDEQLERVHEASK
                     KTAVLRSANMSLGVNTLFKVLKSMTKLLADAGFDIDIVEKHHRRKLDAPSGTAIALAE
                     AVNEPLNNEYEFVYDRSQRREQRPKKEIGISAVRGGTIVGEHEIIFAGQDEVIELKHT
                     AYSRAIFGKGAVSAALYLAGKEAGMYDMSDVIG"
     gene            complement(3314..4198)
                     /gene="dapA"
                     /locus_tag="PLC71_06110"
     CDS             complement(3314..4198)
                     /gene="dapA"
                     /locus_tag="PLC71_06110"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRM02248.1"
                     /translation="MSIFTGAGVALVTPFKEDLSVDYDQLEKFIDFQIDNGTDSIVIC
                     GTSGEASTMSHDEQIEVVSACVSHVNGRVPVIAGAGANCTDEALNLAKRSEKAGADGL
                     LVVTPYYNKATQKGLEEYYTTVGNSVDIPIIMYNVPGRTGTNIQPATAVKIAKSVDNI
                     VAIKEASGDIGQVATLAALADGCLDIYSGNDDQVVPLLALGGKGVISVLSNVAPRETH
                     DMVMKFLEGDVKGSLDIQLKYMDVIHNLFSEVNPIPAKRAVAEMGYCKNIVRRPLTEM
                     EEDHAQVLIQSMKEAGIL"
     gene            complement(4235..4723)
                     /locus_tag="PLC71_06115"
     CDS             complement(4235..4723)
                     /locus_tag="PLC71_06115"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRM02249.1"
                     /translation="MEGRVEIYCGQGRGKTSCAIGQCVKAAGQEKQIIMVQFLKGKET
                     EELSFFKRLEPEVQLFRFEKYERLYEDLNEEEKKEQDHFIYNGLKYAHKVIDTRQCDM
                     VVLDEVLGLLDLGLLKTEELVEMLENRSDDQTIIMTGRQIPEALIPYADAVYEISTIK
                     ES"
     gene            complement(4805..5440)
                     /locus_tag="PLC71_06120"
     CDS             complement(4805..5440)
                     /locus_tag="PLC71_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRM02250.1"
                     /translation="MTEQGINHNQVEVCGRINSAFEYSHEIFGEGFYTVKLLVNRLSE
                     ATDEIPLLISERLVDVTKDYKGMLVRACGQFRSFNKHEENRNHLILSVFVRDLEFIES
                     MDGVRPNQIILDGFICKQPVYRMTPLGREICDILLAVNRSYGKSDYIPCICWGRNARF
                     AGNLEIGSRIQLIGRIQSREYQKRVSENELIRRVAYEVSVNKLEFHEKDEE"
     gene            complement(5580..>5918)
                     /locus_tag="PLC71_06125"
     CDS             complement(5580..>5918)
                     /locus_tag="PLC71_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRM02251.1"
                     /translation="EAGETITYGLFNAQERGTLFVGPGEKVYGGMVIGQTGKTEDIEI
                     NVCRSKKLTNTRASGSDDALKLSPPKILSLEQALEFIDTDELLEITPENIRIRKKILD
                     PTMRKRAKFS"
BASE COUNT         1528 a         1350 c          939 g         2101 t
ORIGIN      
        1 agtaccagct gaaccaatgt tccgaatgct actttctctc catgatacat cttatgtgtt
       61 tctggaattg ctgtaaatcc gttgtggatc gcatgagccc ctgctaatcc tccactctca
      121 aatccgattc ctgataaata agtattagct tcaataacat tctccacagc ttttgtacaa
      181 attccttctt tagctgctat cattgcctgt actccatttt ccattaatgt atcatagcat
      241 aattgcgcaa gattcattgc tgccaatgta catttacctc caacacagtt acttgcatca
      301 gatcttttac atgctctcgc ttcaaaatat gtcgcaagtg catcccccat tcctgagatc
      361 aacagtctta ctggtgcctt acagacgata tcggtatcta ccatgaccat atctgggctt
      421 gatggcaaga acagatattt ttcaaaaaca ccttcatctg tatagataac agataatgca
      481 ctgcatggtg catccgtaga tgcgatcgtt ggcacgatga tcactggttt ctttgtataa
      541 taggcaaccg cttttgatgt atcatgaatc ttaccaccgc caattccaat gatcacatca
      601 cttcctgctt cttccacgat ctttataatt cggttgatct ccgtcataca gcattctcca
      661 ttgaatacat cataaacaag attgctgtct ttatttcctt cattgatcgc cggttctact
      721 ctttttcttc cagatgcact cgtcagaata aagagattct ttccaaattt atctgcataa
      781 gaacaaagat tcttcatctc tcctttacct tgaatgtagc gtgttggact tccaataatt
      841 gctgccataa tatttccctc catatcaact ctatttctca tcatgatacc atggtcaaaa
      901 taccatttga tatcaacatc tcgttattat tataacactc tttttgttat atttaaacat
      961 aaatatattt atattcattc tttttttatc tgtttttatc cgtaagtttg ttattttttt
     1021 atttctcacg ttaatatatt gtcaaatttt cgtttagatt ttttctattt tcttgccttt
     1081 tttatcattt ttccatatac ttatacatac tgaatacaaa atccgacaaa aggagacatt
     1141 tacaaatgat gatcaaaact ttatttttgc taagctttct cttctttatc tactcattta
     1201 tcggctggat cttagaagtg gtacaatcag catttcatca gaaacgtttg gtaaaccgtg
     1261 gttttatcaa ctcaccgctt tgtatttcct atggaatcgg agctatcgtt attacgatca
     1321 atacgcatca gatgaccggc ctatggatct ttgctgccgc catgatcgat gccacagtca
     1381 ttgaatggtt tggcggacat tttattgaac atttttacca tgaacgctgg tgggattatt
     1441 ccaaaaataa atggaatctt gatggctaca tctgcctgtc ccattcggtt ttctggggac
     1501 ttttaggata tatcggagta aagtttgtca atccactgct ctttaaattt tatcatctga
     1561 tcccgccatt tatccggcat cttatcattt ttatccttct tgcagtcctg atcattgata
     1621 ttttagcaac cacgatcgtt gtctttggaa aaaatatcga taaacgacgt tgggaatctg
     1681 cggatgctta tcttacaaag atcagcgtga aattgagttc gttgataaca tcttatgtgg
     1741 atcgtcgtgt tgaacgtgct tatccacagc gtaaattaaa actgccaacg atcccaaaga
     1801 ctggtgtatt tgcccaggga tgcggttttt ataaggtgtt tttgttattt tccatcggtt
     1861 ctttgcttgg agatatcatt gaaacgatct tctgtcgttt gaagatgggt gtctggatga
     1921 gcaggagcag ccttgtatgg ggtcctttta gcattgtgtg gggatttgcc tttgctggtg
     1981 ttacattgct tctataccgc tataaagacc gttcagacag ctttctattt ttaactggta
     2041 cttttcttgg cggcgcctat gaatatctct gcagcgtttt aagtgaaatc gtcttcggaa
     2101 aggttttctg ggattacagt aaaatgcctt ttaacttgaa tggaagaatc aatcttcttt
     2161 attgtttctt ctggggaatt gcaaccgttg tctggtttaa gaggatttat cctttcttat
     2221 ccaatctgat cgagaaactt ccgattgcat ttggaactgt atttacatgg atcattgtcg
     2281 ttttcatggt attaaatatg ttcatgtcac tatctgcctt gatccgttat gatcagcgta
     2341 acaaaaaaat acctgcttct aatttctttg aacgatatct ggatacccat tataacgacc
     2401 agaaaatgaa actcatctat cctaaagcaa agaaagtgca ttgattgaga tacttttaca
     2461 aaaacttctg atgcagaaaa agggaatgaa actaacattt tgttgtttca ttcccttttc
     2521 ttatcttttc tttaaaatat gatcctatcc gatcacatca ctcatatcat acatacctgc
     2581 ttcttttcct gcaagataaa gagctgcact gactgctccc tttccaaaaa tagctctgct
     2641 gtatgcagta tgttttaact caataacttc atcctgtcct gcaaaaatga tctcatgttc
     2701 tccaacgatc gttccaccac ggactgctga gattccaatc tctttctttg gacgctgttc
     2761 tcttctctgg cttctgtcat atacgaattc atattcgtta ttcaacggct cattgacagc
     2821 ttctgcaagc gcgattgctg ttccagatgg tgcatctaac tttcttctgt gatgtttctc
     2881 cacgatatca atatcaaatc ctgcatctgc taataacttt gtcatagatt ttaatacttt
     2941 gaataatgtg ttaactccta atgacatatt cgcagaacga agaactgctg tcttcttact
     3001 tgcttcatga actctttcta actgttcatc gcttaatcct gttgtacata acacaactgg
     3061 aatctgtctc tctactgcaa aatccagtaa gccatcaacg gctggtgctg cggaaaagtc
     3121 gatgatcgca tcaacttcta catcacacgc attgatatct gtaaatacag gataatcgtt
     3181 gtgcccatca tccacacggt caacaccgca tgccataaca gctccttctg tctcttctac
     3241 gatcttgctg atcacctgtc ccatgactcc attgcagcca tgcatcataa tcttaaccat
     3301 cttttgtaac tccttaaaga attcctgctt ctttcattga ctgaattaat acctgtgcgt
     3361 gatcctcttc catctctgtt aatggacggc gaacgatatt tttacaatat cccatctctg
     3421 ctactgcacg ttttgcagga attgggttta cttcgctaaa taagttatgg atcacatcca
     3481 tgtattttaa ctggatatct aaacttcctt tgacgtcccc ttctaagaac ttcattacca
     3541 tatcatgagt ttctcttggt gctacattgg ataatacaga gatgacacct tttccaccta
     3601 atgctaaaag aggtacaacc tgatcatcat ttcctgaata aatatctaag catccgtctg
     3661 ctaatgctgc aagagttgct acctgaccga tatctccgct tgcttctttg atcgctacga
     3721 tattatcaac actcttcgcg atcttcactg ctgtagctgg ctggatgttt gttcctgttc
     3781 ttcctggcac attgtacatg atgattggaa tgtctacaga attaccaact gttgtataat
     3841 attcttccaa acctttctgt gttgctttgt tatagtatgg tgttacgact aacagtccgt
     3901 ctgctcctgc tttctctgat ctcttggcaa ggttcagtgc ttcatctgtg cagttcgctc
     3961 ctgctcctgc gataactgga actcttccat tgacatgaga aacacatgca ctgactactt
     4021 cgatctgctc gtcatgagac attgtagaag cctctcctga agttccacag ataacgatac
     4081 tgtctgttcc gttatcgatc tggaaatcaa taaatttctc taactgatca taatccaccg
     4141 ataaatcttc tttgaatggt gtcactaatg caacacctgc acctgtaaaa attgacattt
     4201 ttctttcctc ctatcatcct atgtagcatc aacattaaga ttctttgatc gtactgatct
     4261 catatactgc atctgcatac ggaatcaatg cttctggaat ctgccgtcct gtcatgatga
     4321 tcgtctggtc atcactacga ttttctaaca tctccaccag ttcttcggtc tttaatagtc
     4381 caagatccaa aagtccaagc acttcatcta aaacgaccat gtcgcactgt cttgtatcta
     4441 tgactttatg tgcatatttt aatccattat aaataaaatg atcttgttct ttcttctctt
     4501 cttcattcag atcctcatac aatctctcgt acttttcaaa acgaaacaac tgtacttctg
     4561 gttctaaacg tttgaaaaag cttaattctt cggtctcctt tcctttcagg aactgaacca
     4621 taatgatttg cttttcctgc ccggctgctt tcacacactg gccgatcgca caggatgtct
     4681 tccctctgcc ctggccacag tatatttcca ctcgtccttc catcttttca cctcaaagaa
     4741 tcttcctttt gtagtaatat atttggttta ggatattata tcatttctga tactataaat
     4801 acaactattc ttcatccttt tcatgaaact ccagtttgtt tactgatacc tcatatgcaa
     4861 ctcgtctgat cagttcattt tctgaaactc ttttctgata ttctctgctt tgaattcttc
     4921 cgatcagctg aattcttgaa ccgatttcaa gattccctgc aaatctcgca ttccttcccc
     4981 agcagatgca tggtatgtag tcggatttgc cataggaacg gttcactgca agcaagatgt
     5041 cacaaatctc tcttcccagc ggtgtcatcc gatatactgg ctgcttacag ataaatccat
     5101 ctaaaatgat ctgattcggt ctgactccat ccatgctttc tataaattcc agatccctga
     5161 cgaatacaga taagatcaaa tgattacgat tctcctcgtg cttgttgaag gaacggaatt
     5221 gaccgcaagc tcttacaagc attcctttgt aatcttttgt cacatcaacg agccgctctg
     5281 atattaataa tggtatctcg tctgttgctt cacttaatct gtttactaaa agtttgactg
     5341 tataaaatcc ttctccgaaa atctcatggc tgtattcaaa cgctgaattg atacgtccgc
     5401 aaacctccac ttgattatga ttgattccct gctctgtcat actctttctc ccttcttgtg
     5461 ttctattagt atactttaaa tcgtattcca attttgacac aattagaaga aaaatccttc
     5521 gacaaagtta gtcacacttt ttcgacatgt tttaaacggc tttcataccg tgcatgatct
     5581 tatgagaatt ttgcacgttt tctcattgtt ggatctaaga tcttcttgcg gatacggata
     5641 ttctctggag tgatctcaag taattcatcc gtatcgataa actctaatgc ctgctctaag
     5701 cttaagatct ttggaggaga taattttaat gcatcatctg atcctgaagc tcgtgtattt
     5761 gtaagctttt tgcttcggca tacgttaatc tcaatatctt ctgtctttcc tgtctgtcca
     5821 atgaccattc ctccgtatac tttctcacca ggtcctacga ataatgtacc tctctcctgt
     5881 gcattaaaca gaccatatgt gatcgtttct cctgcttc
//
LOCUS       DAOMUE010000110         5916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3643_length_5916_cov_2.396861, whole genome shotgun sequence.
ACCESSION   DAOMUE010000110 DAOMUE010000000
VERSION     DAOMUE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3643_length_5916_cov_2.396861"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1215)
                     /locus_tag="PLC71_06130"
     CDS             complement(<1..1215)
                     /locus_tag="PLC71_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005416 - amino acid:monoatomic cation
                     symporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid carrier protein"
                     /protein_id="HRM02252.1"
                     /translation="MLSKIISDVDAFVWGPVMLVLLVGTGILLTIRCNFLTWRNLPWA
                     IKKTLSKESRTKNRGEGDVSPFSALTTALAATIGTGNIVGVATAMVSGGAGALVWMWI
                     SACFGLSSKFSECMLAIKYREVNEKGEMSGGPMYTMKKALKNKKLGAVLGWLFALFAV
                     IASFGIGNMTQGNSISTAVHETFGVSVSTVGAVITILALLIIIGGIKTISKVSSVVVP
                     VMAIFYVIAGVIVILGNISNIPAGLSMIFHMAFSVKAVGGALCGNIVASMMNAARYGV
                     ARGCFSNEAGMGSAAITAAAATTDHPVRQAYINMTGTFWDTIVVCTITGLAIASSGVL
                     GQIDPATGEMYIGSALTIAAFSTVLGKVGGYLVTIGIALFAFSTILGWEYHGEKAFEY
                     LMGTHKFNMIYRI"
     gene            complement(1348..>2016)
                     /locus_tag="PLC71_06135"
     CDS             complement(1348..>2016)
                     /locus_tag="PLC71_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRM02253.1"
                     /translation="HIDMSEYEKKNYKSYNEFFSRKIKEGKRPFPEDKTILGSPCDCK
                     VSVYPIEEKTSFVVKDTRYTLDSLIRNRKIARHFQGGYAVILRLTVDDYHRYCYFDDG
                     IKSENHRIDGVYHTVNPIANDHVKIYKENTREYTLMKTKHFGDALQMEVGALMVGKIV
                     NHDGAGSMRRGIEKGYFQFGGSTIILLLEKDKVEIREELLERTKNQCETKIRQGEMIG
                     KALV"
     assembly_gap    2017..2026
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2027..2224)
                     /locus_tag="PLC71_06140"
     CDS             complement(<2027..2224)
                     /locus_tag="PLC71_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRM02254.1"
                     /translation="MRYADRDGNIYEEENGQDRLLHWMYTTMAGRASLKILIRPWVSK
                     LGGAFLSSPISKCLIKGFVKNN"
     gene            complement(2244..2855)
                     /locus_tag="PLC71_06145"
     CDS             complement(2244..2855)
                     /locus_tag="PLC71_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRM02255.1"
                     /translation="MIGFYNASVILTYLGVVSAVFGMSQAFNGNFKIAILCLMISGLT
                     DMYDGTIAKMIKRSEDAKKFGIEIDSLCDLVCFGVYPVVIGYCLGMDQWYARIIYMCY
                     VLGGVIRLAYFNVTEEQRQAHSSSRRKHYQGLPITSVALIIPIVYQLRRFFPEEFPYI
                     YVAMLAVIAFLFVFDFKVFKPDKKCMLAFVVIGALIAVKMFLL"
     gene            complement(2868..3893)
                     /locus_tag="PLC71_06150"
     CDS             complement(2868..3893)
                     /locus_tag="PLC71_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRM02256.1"
                     /translation="MKNKKKVIGNTVFFFALFGLTAYAVLKGKELSELAEVLKSVKPA
                     YYIAGLVMIVVFVCCESYIIYRMLRLLHYNDVPKRNCVKYSFVGFFFSCITPSATGGQ
                     PMQLYYMNRDKVDISVGTVILMIVTILYKFVLVFLGALIFLFRHFLVAEYIGKAAFFF
                     YLGMALNVFCVAFMLILVFEPTLASKIVLWLFQKLEKFHILKKKEERLEKLQNSMIEY
                     HKAAAIIQGHRIFIFRMFVVTLIQRLIHFSITYLVYRAMGLSALGFIDVVALQSVISI
                     SVDMLPLPGGMGISEGLFLNIFKKIFVGGLLYPAMLLSRGISYYALVLISAVMTGVAH
                     VTIKRKE"
     gene            complement(3899..4627)
                     /locus_tag="PLC71_06155"
     CDS             complement(3899..4627)
                     /locus_tag="PLC71_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRM02257.1"
                     /translation="MNQKKDYVLRWIPKYSIIPLITALVLNTLVYSGTMVLCKGLYHH
                     DFTTAFDRMVPVIPEFTSIYLICYVFWVVNYIMTARISREHMYRFLTADYLSRIVCGI
                     FFVFLPTTLVRPEITGTGFWDQALRFVYSIDQSANLFPSIHCLVSWFCYIGIRHQKKI
                     PVWYQRFSLVFAILVCISTQVTKQHYIIDVFGGIILSEVCYLIGRKTNLYQGLWKVFE
                     KINQRVGLERRSVKYKGKLSEIKG"
     gene            complement(4788..>5916)
                     /locus_tag="PLC71_06160"
     CDS             complement(4788..>5916)
                     /locus_tag="PLC71_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRM02258.1"
                     /translation="EVQESSMDMSALGGSGISVQIKGNDLNKLRSIGKDIAQIVEDTK
                     GTQNISNGSEETTPDLHVVVDKKKAVKNGLTVATIYQQLSEKLKDASEATTITINEKE
                     YSVNVGNSAKNTLTRDDLKKVKLTGTVSGKEKEVTLDQVANFRNSDSLSSINRNQQER
                     TLSVTSEIKDGYNVGKVSSAVQKKIKKYNAPKGYSITMKGEMESIQETTGQIGLMLLL
                     AVAFMYLIMVAQFQSLKSPFIILFTIPMAFTGGFLGLLITGKDLSAIAMIGFVMLAGI
                     IVNNGIVLVDTINQLRESGYEFEEAILTAGTMRVRPILMTALTTILGLSTMAIGLGQG
                     AEMMQPMAIVTIGGLIYGTLLTLLVVPCIYGLFNRRKKKAE"
BASE COUNT         1751 a         1390 c          887 g         1878 t
ORIGIN      
        1 aattcggtag atcatgttga acttatgagt tcccatcaga tactcaaacg ccttttctcc
       61 atgatactcc catccaagaa ttgtggaaaa tgcaaaaagt gcaattccga tcgttaccag
      121 atatcctcca acttttccaa gaactgtgga aaatgccgca atcgttaatg cagaacctat
      181 gtacatctct cctgttgccg gatcgatctg tccgagcact ccggagcttg cgatcgcaag
      241 acctgtgatc gtacagacaa cgatcgtatc ccagaaagtt cctgtcatat tgatatatgc
      301 ctgccgaacc ggatgatctg ttgttgctgc tgccgctgtg atcgctgctg atcccattcc
      361 agcttcattt gaaaaacatc ctctggcaac tccgtaacgt gctgcattca tcatagatgc
      421 gacgatattt ccacaaagag ccccaccaac cgctttcaca ctaaatgcca tatgaaagat
      481 catagaaagt cctgctggaa tattactgat attcccaaga atgacgatga ctcctgcgat
      541 cacataaaag attgccatca ctggaactac gacagaagat acttttgata tcgtttttat
      601 tccaccgatg atgatcagta aggctaaaat agtgatcact gctccaacag tactaaccga
      661 tactccaaat gtctcatgaa ctgctgttga aattgaattt ccctgtgtca tatttccaat
      721 tccaaaggat gcgatcacgg caaataaagc aaacagccat ccaagtacgg ctccaagctt
      781 tttgttcttg agtgcctttt tcattgtata cattggtcca ccggacatct cgcccttttc
      841 attgacttct ctgtatttga tcgccaacat acattctgaa aatttagatg ataatccaaa
      901 acatgcagaa atccacatcc acaccaatgc tccagcacct cctgaaacca ttgctgtcgc
      961 tactcctacg atatttccag ttccgatcgt tgccgccagt gctgttgtca atgctgaaaa
     1021 tggtgataca tcaccctctc ctctgttttt tgttctggat tctttactta atgtcttttt
     1081 aatcgcccaa ggaaggtttc tccaggttag gaaattgcat cgaattgtta aaaggattcc
     1141 tgttcccacg agaagcacca gcatcactgg tccccataca aaagcatcca catcagatat
     1201 gatcttactt aacatatact atctctcctc tctgatctta ttttcatttc taaaatgcaa
     1261 aaaaggagca acgaaaattc gttgctcctt caacttatga acttagtata gccatatatg
     1321 accactctgt caatatttat tttaaggtca taccaatgct ttcccgatca tctcaccctg
     1381 cctgatcttt gtctcacact ggttcttggt acgttctaac agttcttctc tgatctcaac
     1441 cttatccttt tctaataata aaatgatcgt tgaacctcca aactgaaaat atcctttctc
     1501 aatcccgcgc ctcatagatc cggctccatc gtgattaaca atctttccaa ccatcaatgc
     1561 tccaacttcc atctggagtg catctccaaa atgttttgtc ttcatcaatg tatattctct
     1621 ggtattctct ttatagattt tcacatgatc atttgcgatc ggattgacgg tatgataaac
     1681 tccatcaatc ctatgattct cactcttaat tccatcatca aaatagcagt atctatgata
     1741 atcatctact gtcaggcgta agatcacggc ataaccgccc tgaaaatgtc ttgcgatctt
     1801 gcggtttctg atcaatgaat ccaacgtata tcttgtatcc tttacaacaa aagatgtctt
     1861 ctcttcgatc ggatacacac ttactttaca gtcgcaagga cttcctaaga ttgttttatc
     1921 ttccggaaat ggacgttttc cttctttgat ctttcttgag aagaattcat tataagattt
     1981 gtaatttttc ttctcatatt cactcatatc aatatgnnnn nnnnnnatta ttcttgacaa
     2041 atcctttgat cagacatttg gagatgggag aacttaagaa agctccccct agtttggata
     2101 cccaaggtct gattaatatt tttagactgg ctcgtcctgc catagttgta tacatccaat
     2161 gaagcaaacg atcttgccca ttctcttctt cgtaaatatt cccatctcta tcggcatatc
     2221 tcatctacga ttctcctatc atcttacagt aagaacatct ttactgcgat caatgctcca
     2281 ataactacaa atgcaagcat acatttctta tctggtttaa agactttaaa atcaaataca
     2341 aataagaaag cgatcactgc taacattgca acatatatgt atggaaattc ttctggaaag
     2401 aatcttcgaa gctgatatac gatcggaatg atcagtgcaa ccgatgtaat tggcagtccc
     2461 tgataatgtt ttcttctgga actgctgtgt gcctgacgct gttcttctgt aacattaaaa
     2521 tatgccagac gaataactcc gcctaaaaca taacacatat aaatgatcct tgcataccat
     2581 tggtccattc caaggcagta accgatcaca accggataca ctccaaaaca gacaagatca
     2641 cataaagaat caatctcgat tccaaacttc ttggcgtctt cgcttctctt gatcatcttt
     2701 gcgatcgttc catcatacat atctgttaat cctgagatca tcaaacataa aattgcgatc
     2761 ttaaagtttc cattgaatgc ctgtgacatt ccaaatactg cactgacaac tcctaaatat
     2821 gtcagtatta cagatgcatt ataaaatcct atcatacctt tttccctcta ttctttacgc
     2881 tttatcgtaa catgtgcgac tcctgtcatc actgcactga tcaataccag tgcataataa
     2941 ctgattcctc ggcttaacag cattgccgga tataataatc cgccaacaaa aatcttctta
     3001 aagatattta aaaataagcc ttcactgatt cccattccgc ctggcaacgg aagcatatcc
     3061 acagaaattg aaataacact ctgtaaagca actacatcaa taaatcctaa tgcagaaagt
     3121 cccattgctc tgtatacaag atatgtaatt gaaaaatgga tcagtctctg aatcaatgtg
     3181 acaacgaaca tccgaaaaat aaaaatccga tgtccttgaa tgattgctgc tgctttatga
     3241 tactcgatca tagaattctg aagtttttca agacgttctt cttttttctt taaaatatgg
     3301 aacttttcta atttctgaaa cagccacagc acgattttgg atgctaacgt tggctcaaat
     3361 acaagaatca gcatgaacgc aacacaaaac acattcaatg ccattccaag gtaaaagaag
     3421 aatgctgcct tcccaatata ttctgccaca agaaaatgac ggaataaaaa gatcagtgct
     3481 cctaaaaata ccagaacaaa tttatataaa atggtaacga tcatcaaaat gacggttccc
     3541 acggatatgt caactttatc gcggttcatg tagtaaagct gcattggctg tccgccagtt
     3601 gcagacggtg taatacaact gaaaaagaaa cctacaaacg aatatttgac gcaatttctc
     3661 ttcggcacat cattgtaatg aagcagccgt agcatgcgat aaataatata cgactcgcag
     3721 cacacaaaca caacgatcat aaccaatccc gcaatataat atgcaggttt tactgatttt
     3781 agaacttccg ccagctcaga aagttccttt cctttcaata cggcatatgc agtcaatcca
     3841 aataatgcaa agaaaaagac tgtattaccg atgacttttt tcttattctt catctttcct
     3901 accctttgat ctccgataat ttccctttgt atttcacaga tcttctctct aaaccaactc
     3961 gttggttaat cttctcaaac accttccaga gtccttgata caaattggtc tttcttccga
     4021 tcagataaca aacttcactt aaaatgatcc cgccaaacac atctatgata taatgctgct
     4081 tggttacctg cgtggaaata cagactaaaa ttgcaaaaac aagtgaaaat ctctgatacc
     4141 acactggaat cttcttctga tgcctgatcc caatataaca aaaccagctt accaaacagt
     4201 gaatcgatgg aaataagttt gccgattgat cgatcgaata cacaaatcgt aatgcctgat
     4261 cccaaaatcc tgttcctgtg atctctggtc ttaccagcgt tgttggcaaa aagacaaaga
     4321 aaatcccaca tacgatcctt gacagataat ctgctgtcaa aaatctatac atatgttctc
     4381 tactgattct cgcagtcata atgtagttca caacccaaaa cacataacat atcaaataaa
     4441 tacttgtaaa ttctgggatc accggtacca tccggtcaaa tgctgttgta aaatcatgat
     4501 gatataatcc tttacaaagc accattgttc cactgtaaac caacgtattc agtactaacg
     4561 ctgtaatcag cggaatgata gaatacttcg gaatccatct cagaacataa tcttttttct
     4621 gattcatatg tctgctcctt ccaatgatct atcctctttc ttttctctct tatgattctt
     4681 ccatatatca aagaatcatg tcaattctga catgattctt gtactattat atcttttctc
     4741 tttttaaaag taaaggaagg cggtcatatt taatagtttt gtaagattta ctctgctttc
     4801 ttttttcttc tattgaagag tccataaata catggaacca ctaataatgt tagtaaagtt
     4861 ccataaatca aaccaccgat cgtaacgatc gccatcggct gcatcatctc ggcaccttgc
     4921 cctaaaccaa tcgccattgt agataatcca aggatcgttg taagggctgt catcaagatc
     4981 gggcgtacac gcatcgtacc tgctgtcagg attgcttctt caaattcgta tcccgattct
     5041 cttaactgat tgatcgtatc taccagcacg ataccattat ttacaatgat acctgcaagc
     5101 ataacaaatc cgatcattgc gatcgcactt aagtcttttc cagtgatcag cagtcctaag
     5161 aatcctcctg taaatgccat tggaatcgta aataaaatga taaatggcga tttcagtgac
     5221 tggaactgtg ctaccatgat caaatacata aatgctactg ccagcaataa catcaggcca
     5281 atctgtcctg ttgtttcctg aatggattcc atctcacctt tcattgtgat actgtatcct
     5341 tttggtgcat tgtatttctt aatcttcttt tgtacagcgc tgctgacttt tcctacattg
     5401 tagccatctt tgatctctga tgttacactc aatgttcttt cctgctggtt tctattaata
     5461 gaagataaac tgtcactatt tctgaagttc gctacctgat ccaatgtaac ttctttttct
     5521 tttccactta ctgttcctgt aagtttcact ttcttaagat catctcttgt cagtgtattc
     5581 tttgcactat ttccaacatt gacactatac tctttttcat tgatcgtgat cgttgttgct
     5641 tcactggcat cttttaattt ctcacttaac tgttgataga tcgttgcaac tgttaaacca
     5701 ttttttactg cctttttctt gtctaccaca acatgaagat ctggtgttgt ctcttcactt
     5761 ccattcgaga tgttctgtgt tcctttcgta tcctctacga tctgggcaat atcttttcca
     5821 atagatctta gtttatttaa atcatttccc ttgatctgta cggaaatacc gcttccacca
     5881 agtgcactca tatccatact ggattcctgc acttca
//
LOCUS       DAOMUE010000111         5904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3654_length_5904_cov_1.949222, whole genome shotgun sequence.
ACCESSION   DAOMUE010000111 DAOMUE010000000
VERSION     DAOMUE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3654_length_5904_cov_1.949222"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLC71_06165"
     CDS             <1..238
                     /locus_tag="PLC71_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HRM02259.1"
                     /translation="KDAKKLYTNCTAAVLRAKECVMDTVATVQENAEDIYAEAQQINE
                     ERAAEAEEWEAYAETDEAEETEEVSEDTFKEVAE"
     gene            306..>1517
                     /locus_tag="PLC71_06170"
     CDS             306..>1517
                     /locus_tag="PLC71_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRM02260.1"
                     /translation="MKFTIKHEIRGRIRIHNCQKTMSADQADELQYYLITKDFVTSVK
                     IYDRTNDIVICYDASRSEILTALKEFYHGIITVPDNYQESQSRQLNQTYQEKLISKVL
                     FHYGNKLLFPYPLRAGITVIKSLKYIYEGIHTLAQGKIEVPVLDGTAIGVSIIRRDIN
                     TASSIMFLLGIGEILEEWTHKKSVDDLAKSMSLNIDKVWKVEEDHEILVPSNEIDAND
                     IVHIQMGNMIPFDGVVVDGEAMVNQASLTGESTPVRKIPDGYVYAGTVVEEGDLKVCV
                     KEVNGSSKFDKIVTMIEESEKLKSSLESKAEHLADRLVPYTLAGTALTYLFTRNVTKA
                     LSVLMVDFSCALKLAMPISVLSAIKEANDHQIVVKGGKYLEAMAEAETIVFDKTGTLT
                     KAEPTVLDIVSF"
     assembly_gap    1518..1527
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1528..2402
                     /locus_tag="PLC71_06175"
     CDS             <1528..2402
                     /locus_tag="PLC71_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRM02261.1"
                     /translation="MKCKELLRIAACLEEHFPHSMAKAVVQAAVDRNLVHEELHSEVE
                     YIVAHGISSMIENKKVVIGSYHFVFEDEQCTIPEGKEELFEKLPEECSHLYMAIEGKL
                     AGVICIEDPLREEAKDVIKALKAAGIKKVVMMTGDSDRTAKVIAAKVGVDEYHAEVLP
                     EDKAAFVEKEKAEGRKVIMIGDGINDSPALSASNVGIAISDGAQIAREIADVTIEGDH
                     LHGLVTLKHISNHLMRRIHNNYRFIVGFNAGLIGAGVLGILQPTTSALLHNTSTLCIG
                     LRSMKNLVSEEELQ"
     gene            complement(2512..2760)
                     /locus_tag="PLC71_06180"
     CDS             complement(2512..2760)
                     /locus_tag="PLC71_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02262.1"
                     /translation="MAMNFNQMLKFQQIKNAFKRFDQDHPKFKNFMNAVWREQALKQD
                     AVIEISITSPEGKNYCSNIKLNAADIEMFEQLKSMKPE"
     gene            2918..>3229
                     /locus_tag="PLC71_06185"
     CDS             2918..>3229
                     /locus_tag="PLC71_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HRM02263.1"
                     /translation="MDFYQQLQLSSIGSKQWIKGAKDSKEKHKRILIYNFKVYLVVAF
                     CFALVTLYSMIFGSQNSVVGVLVLLVLMILRQVDFGIDTKHSIGVIFMIFAILAVGPR
                     LA"
     assembly_gap    3230..3239
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3240..>3956
                     /locus_tag="PLC71_06190"
     CDS             <3240..>3956
                     /locus_tag="PLC71_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HRM02264.1"
                     /translation="HLGIIPTKQGKHLNLFIHFLCIMAIMILSCHNVIMSNQSTFILG
                     YLLFYGYDVTGHNYVLRCCGLFAGAVICSLVFYKNHRNRTFHRGFYHLFKEFHLFSAR
                     SSWYLRLSLGISTAMLIGELVHMPRVMWIGIAAMSVLLPFSKDMKYRVQRRGPFNILG
                     CMIFLILHAILPDHIFQWIGLIGGIGVGYSAGYAWQTVFNTFGALYIAENLFGLKNAI
                     ILRIAANVFGSLYAYGFDQLF"
     assembly_gap    3957..3966
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4096..5064
                     /gene="bsh"
                     /locus_tag="PLC71_06195"
     CDS             4096..5064
                     /gene="bsh"
                     /locus_tag="PLC71_06195"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HRM02265.1"
                     /translation="MCTAATYKTKDFYFGRTLDYEFSYGDEVVITPRNYPFHFRHIDA
                     IESHYAMIGMAHVVDNDPLYYDAFNECGLAMAGLNFVGNAAYYDIEEGKDIAQFEFIP
                     WILGTCANLEEAKTALIHMNLTNTPYSEQFPLAQLHWIIADQSGAITVEAMEDGMHIY
                     ENNVGVLTNNPPFNIQMFLLNQYMNLSPKQPENLFAKDIDLDQYSRGMGAIGLPGDLS
                     SSSRFAKVAFTKLHSVSGDSENESVNQFFHILGSVDQQRGCCDVNGKYEITLYTSCCN
                     TQKGIYYYTTYDNHQISAVDMTKEDLNTKNLICYEVITGEHIQMQN"
     gene            5109..5666
                     /locus_tag="PLC71_06200"
     CDS             5109..5666
                     /locus_tag="PLC71_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRM02266.1"
                     /translation="MEVRLATPQDAEELLEIYRYYVEHTAITFEYETPSITEFRQRIK
                     QTLKRYPYLAAVDGHGKIVGYAYASSFKNRAAYDWSVETTIYVDKDVKRQGIGKKLYE
                     ALEEILRKQHVINLDACITDPEIEDEYVTKNSVQYHEHLGYHMVGKFYKSGYKFGRWY
                     DMVWMEKYIGKHKEHPEAFIPFSIL"
BASE COUNT         1969 a          945 c         1156 g         1804 t
ORIGIN      
        1 caaggatgca aaaaaactgt ataccaactg tacagcagct gttcttcgtg caaaagaatg
       61 tgtaatggat acagttgcaa ctgtacagga gaatgctgaa gatatctatg ctgaagctca
      121 gcagatcaat gaagaaagag cagcagaagc agaggaatgg gaagcttatg ctgaaactga
      181 tgaggcggaa gaaacagaag aagtttccga agatactttc aaagaagttg cagaatagaa
      241 tagaacatta agattaaggc acctgcagtt ttcctgcagg tgttttcaaa caaaaaggag
      301 tttgtatgaa atttacgatc aaacatgaga ttcgaggcag aattcgtatt cataactgcc
      361 agaagacaat gagtgctgat caggcagatg aacttcaata ttatcttata acaaaagatt
      421 ttgtaacatc tgtaaaaatc tatgaccgca caaatgacat cgtgatctgt tatgatgcca
      481 gccgcagtga aattcttact gctttaaagg aattttatca tggaatcatc acagtccctg
      541 ataattatca agaaagtcag agcagacaat taaaccagac atatcaggag aaacttataa
      601 gtaaagtctt atttcattat ggaaacaaat tattatttcc atatccatta agagcaggga
      661 tcacagtgat caaatctttg aaatatatat atgaaggaat tcatacactt gctcagggta
      721 agattgaagt tccagtactt gatggtacgg caatcggagt atcgatcatt cgaagagata
      781 tcaataccgc atcctctatc atgtttttac tcggaatcgg agaaattctc gaagaatgga
      841 cacataagaa atccgtcgat gatcttgcaa aaagcatgtc cttgaatatt gataaggtat
      901 ggaaagtcga agaagatcat gagattttag ttccatccaa cgaaattgat gctaacgata
      961 tcgttcacat acagatggga aatatgatcc catttgatgg agttgttgtt gatggtgaag
     1021 caatggtcaa tcaggcatca ttaactggag aatcaacacc tgttcgcaag attcctgacg
     1081 gatacgtata tgcaggaact gtcgttgaag agggtgactt aaaagtttgc gtaaaagaag
     1141 taaatggatc aagcaaattt gataagattg ttacgatgat cgaagaatct gaaaaattaa
     1201 aatcttcttt agaaagtaaa gcagaacatc ttgcagatcg tctagttcct tatacattgg
     1261 ctggaactgc tctcacatat ttgtttacaa gaaatgtaac aaaagcttta tctgtactga
     1321 tggttgattt ctcatgtgca ttaaaacttg caatgccaat ctctgtttta tctgcgatca
     1381 aggaagcaaa tgaccatcag atcgttgtca aaggtggtaa atacttagaa gcaatggcag
     1441 aagccgaaac gatcgtcttt gataaaacag gaactttaac aaaagcggaa cctactgttc
     1501 ttgatattgt ttcatttnnn nnnnnnngaa tgaaatgcaa ggaattatta agaattgccg
     1561 catgtttaga ggaacatttc cctcattcta tggcaaaagc tgtagttcag gcagctgttg
     1621 acagaaatct tgtacatgaa gaattacatt ctgaggttga gtatatcgtg gctcatggaa
     1681 tttcttccat gatcgagaat aaaaaagttg tcattggaag ttatcatttt gtattcgaag
     1741 atgagcagtg tacaattcct gaaggcaaag aagagttatt tgagaaactg cctgaagaat
     1801 gttcgcattt atatatggcg atcgaaggaa aacttgcagg tgtaatctgt attgaagacc
     1861 cattacgtga agaagccaaa gatgtgatca aagcattaaa agctgctgga atcaagaaag
     1921 tcgttatgat gactggagac agtgatcgta ctgcaaaggt tattgcagcc aaagttggtg
     1981 tggatgaata tcatgctgag gtacttccag aggataaagc tgctttcgtt gagaaagaaa
     2041 aagccgaagg aagaaaagtt attatgatcg gagatggaat caatgattct ccagcattat
     2101 ctgcatctaa tgttggaatc gcgatcagtg atggagctca gattgcaaga gaaatcgcag
     2161 atgtaacgat tgaaggagac catttacatg gtctggtaac tctcaaacat atcagtaacc
     2221 acttaatgcg ccgaattcat aataattacc gctttattgt tggatttaac gcaggactta
     2281 ttggtgctgg ggttttagga atcttacagc caacaacatc tgcattgtta cataatacat
     2341 cgaccctttg tattgggctt agaagcatga aaaatctggt gtctgaggaa gaattacagt
     2401 aaaaatcaag aaggttagtc gtgaaatttg actagccttc tttttgagtt gtgacattaa
     2461 atttttgctt ttcgggattc cgtgacatta atttgacatt aaaatttgaa attattctgg
     2521 tttcatggat ttcaactgtt caaacatctc gatatctgct gcattcagtt tgatattcga
     2581 acaataattt ttcccttctg gagatgtaat agaaatctcg atcacagcat cctgtttaag
     2641 tgcctgctct ctccatacag cattcatgaa attcttgaat tttggatggt cttgatcaaa
     2701 ccgtttgaat gcatttttta tctgctgaaa ctttaacatc tgattaaaat tcattgccat
     2761 gaggataaat cctcctttct ttatctgtcg ctttcataaa taaatcatat caaaaataag
     2821 aaaaaacgtc aaataacgta ttgacgaaca gcctagaagt gagtaaaatt gttcattgct
     2881 actcagaaac agattcttat cacaggaggt attaacattg gatttttatc aacagttaca
     2941 attaagttcc atcggttcca aacaatggat caaaggtgcc aaagattcaa aagagaaaca
     3001 taaacgtatc cttatctata attttaaagt atatttagtt gttgcatttt gttttgcatt
     3061 agtaactctt tacagtatga tctttggatc acaaaacagt gttgtcggcg tgctagtctt
     3121 actggtttta atgatcttac gacaggttga cttcggaatc gatacaaaac acagcattgg
     3181 cgttatcttt atgatttttg caatccttgc ggttggacca agacttgctn nnnnnnnnnc
     3241 atttgggaat catacccact aaacagggta aacatcttaa tctttttatt cattttcttt
     3301 gtattatggc gatcatgatc ttaagctgcc ataacgttat catgtccaat cagtccacat
     3361 ttatcttagg atatttgtta ttctatggat atgatgttac agggcataac tatgtgttaa
     3421 gatgttgcgg attatttgca ggtgctgtga tctgttccct tgtattttac aagaatcata
     3481 gaaatcgaac atttcataga ggattttatc atttatttaa ggaatttcat ctgttttctg
     3541 caagaagcag ctggtattta agattatctc ttggaatttc tactgcaatg ttgatcggag
     3601 aattggttca tatgccaaga gttatgtgga tcgggattgc tgcaatgtct gtcctgttgc
     3661 cattttcaaa agatatgaaa taccgtgtac aaagaagagg accatttaat attcttggat
     3721 gtatgatctt tcttatttta catgcgatcc ttcctgatca tatctttcaa tggatcggtc
     3781 tgatcggcgg gataggagtt ggttattctg ctggatatgc ctggcaaacc gttttcaaca
     3841 cctttggggc tctttatatt gcagaaaatc tttttggcct gaagaatgcc atcatcttaa
     3901 gaatcgctgc caatgtcttt ggctctcttt atgcttatgg atttgaccag ttattcnnnn
     3961 nnnnnnaaat ctttttgaaa ataattccat tatgacagat caggcataac atctttgaat
     4021 gataccttag aaacaggata taattattac tgaaaatcat tgaaattaat taatagaagg
     4081 aggagtaaaa gagcaatgtg tacagctgca acttacaaga caaaagattt ttattttgga
     4141 agaactttag attatgaatt ctcttatgga gatgaggttg tgatcactcc aagaaattat
     4201 ccatttcatt ttagacatat cgatgccata gaaagtcatt atgctatgat cggaatggct
     4261 catgtagtcg acaatgaccc tttgtattat gatgcattca atgaatgtgg actggcaatg
     4321 gctggactta attttgttgg aaatgcagca tattatgata tagaagaagg gaaagatatc
     4381 gcacagtttg aatttatccc ttggattctt ggaacttgtg caaatcttga ggaagcgaag
     4441 acagcattga ttcatatgaa tcttaccaat accccttaca gtgaacaatt tccattagcg
     4501 cagcttcact ggatcattgc agatcaatct ggtgcgatca cagtcgaagc aatggaagat
     4561 gggatgcata tctatgaaaa taacgttggg gtattaacaa ataatcctcc atttaacatt
     4621 cagatgttct tattaaatca atatatgaat ctttctccaa aacaaccaga aaatctattt
     4681 gcaaaagata tcgatttaga tcaatacagt cgtggaatgg gtgcgattgg tcttcctgga
     4741 gatctttctt cttcctcacg ttttgctaaa gtagcattta ccaaattgca ttctgtctct
     4801 ggagattctg aaaatgaaag tgtcaatcag tttttccata ttcttggatc tgtcgatcag
     4861 caaagaggat gctgcgatgt caatggaaaa tatgagatta ccttatatac ttcttgttgt
     4921 aatacgcaaa aaggaatcta ctattataca acctatgata atcaccagat cagtgctgtt
     4981 gatatgacaa aagaagattt aaacacaaaa aatctgattt gctatgaagt gatcactggg
     5041 gaacacattc aaatgcaaaa ttaaatgaaa aagcaccact atttcattaa actgaaagga
     5101 atgacaaaat ggaagtcaga ttggcaactc cacaggatgc agaagaatta ctggaaattt
     5161 accgctatta tgtagaacat acagcaatca catttgaata tgaaacacct tcgataacag
     5221 aattccgtca acggatcaaa cagacattaa aacgctatcc ttatctggcg gctgttgatg
     5281 ggcatggaaa gattgtcgga tatgcatatg catcatcttt taaaaaccgt gctgcatatg
     5341 actggtccgt agaaacaacc atttatgtag ataaggatgt aaaacgacag ggaatcggga
     5401 aaaaattata tgaggcatta gaagaaatcc ttcgaaaaca acatgtgatc aatttggatg
     5461 cgtgcattac agatccggaa atcgaagatg aatatgttac aaaaaacagt gttcagtatc
     5521 atgaacatct tggttatcat atggttggaa aattttataa aagcggctat aaattcggac
     5581 gttggtatga tatggtctgg atggaaaaat atattggaaa acataaagaa catccagaag
     5641 catttatccc tttttctatt ttataaagag tttaaaactt ttaaaaatca ggaaaaactg
     5701 atataatgaa atataaatat gctaaacaaa agaaaacagg agtatttaat tatgccagta
     5761 tttgatttta gtaacgatac atcagaaata aaagaagaag tagtctgtac ttatacaaca
     5821 ttttcttcga atgtagatca tgctacgata gaatgcccta tcttgatttt atcaaacaag
     5881 aagaagcagt gggagcatca ttcc
//
LOCUS       DAOMUE010000112         5844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3709_length_5844_cov_2.197789, whole genome shotgun sequence.
ACCESSION   DAOMUE010000112 DAOMUE010000000
VERSION     DAOMUE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3709_length_5844_cov_2.197789"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="PLC71_06205"
     CDS             complement(<1..150)
                     /locus_tag="PLC71_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HRM02267.1"
                     /translation="MIYGEEIIEEVRSRNDIVDLISTYVPLKKKGSSYFGLCPFHNEK
                     SPSFSV"
     gene            complement(214..1221)
                     /locus_tag="PLC71_06210"
     CDS             complement(214..1221)
                     /locus_tag="PLC71_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRM02268.1"
                     /translation="MNIREKQEQTEMELLSPYATQSIRTKGRKKPEVECEIRTCFQRD
                     RDRIVHCKAFRRLKDKTQVFLSPVGDHYRTRLMHTLEVSQNARTIARALDLNETLTEA
                     IALGHDLGHTPFGHAGERVLNEMNPAGFKHNEQSVRVAQCLEKNGQGLNLTWEVLDGM
                     KNHSMHSMPHTLEGKIVRLADKIAYINHDIDDSVRAKVLREEDLPKEFTEVLGTTYRE
                     RINTMVLDIVKHSENLNDIVMSKEVRDAMIGLRKFMFERVYEEPNTKKEEDKIKNIIG
                     PLYEYYLIHVEQLPEYNKKMIDKPGDVPVAVCDYIAGMTDHFAIEKYTEIFIPKFFMQ
                     K"
     gene            complement(1277..2047)
                     /locus_tag="PLC71_06215"
     CDS             complement(1277..2047)
                     /locus_tag="PLC71_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 domain-containing protein"
                     /protein_id="HRM02269.1"
                     /translation="MDKNLFLKNMEEMIQIAKTNGNQIDHKELLDYFSDYELNEEAKK
                     LLIASFVEAGIRVLGVDEAQIVAEEEAKEKADISEEEQGAIRFYEEELEQMDLPGEEE
                     QKELIHAWLVEKGDGEAVIESFLPQILEIARTHTGKGVLFGDLVQEGNIGLLEAMAIY
                     QGKDAEGFLAHAKSAVEDSILDAIAMQRGSDSVGEAMAIKANRLDDASTFLSKELGRE
                     PKIEELAKYLSMTEEEVKEVMKISLDALSVMEADIKQS"
     gene            complement(2062..2889)
                     /locus_tag="PLC71_06220"
     CDS             complement(2062..2889)
                     /locus_tag="PLC71_06220"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRM02270.1"
                     /translation="MDIILLLKTILLGIVEGITEWLPISSTGHLILADEFIKLNMTAD
                     FKEMFDVVIQLGAIMAVVVLYFHKLNPFSPKKNHEEKMDTIILWTKVILAVFPAAIIG
                     ILFDDWLNDHFYNPPVVAAMLIIYGVLFIIIENRNKRSRRRPMNDLSRLSYGMALGIG
                     VFQILALIPGTSRSGATILGGILLGCSRTVAAEFSFFLGIPVMFGASLLKIVKFGLAF
                     TSTQLIVLIVGMVVSFLVSIGAIKFLLGYIKKNDFKAFGVYRIILGLLVIVYFAFIK"
     gene            complement(2959..3201)
                     /gene="rpoZ"
                     /locus_tag="PLC71_06225"
     CDS             complement(2959..3201)
                     /gene="rpoZ"
                     /locus_tag="PLC71_06225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRM02271.1"
                     /translation="MLHPSYTELMKVINGDSEEEKVISSRYSIVLASAKRARQIIAGD
                     MPLVEEKDGAKPLSTAVEELWEEKVKILGDDEEAEE"
     gene            complement(3213..3830)
                     /gene="gmk"
                     /locus_tag="PLC71_06230"
     CDS             complement(3213..3830)
                     /gene="gmk"
                     /locus_tag="PLC71_06230"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRM02272.1"
                     /translation="MEKRGSLLVISGFSGVGKGTVAKKLVEKYGYSLSISATTRQPRE
                     GEVDGREYFFKTVDDFKNLIDYNGFIEYARYVDNYYGTPRKFVEDELAAGHNVILEIE
                     VQGAFNIKKQYEDALLIFITAPSAAAIKERLVGRGTESEEVINKRLNRAKEESEDMDK
                     YDYIVINDQVEDCADRIHAIVQAKECLLGNNLKFIEATKKELEQL"
     gene            complement(3849..4730)
                     /locus_tag="PLC71_06235"
     CDS             complement(3849..4730)
                     /locus_tag="PLC71_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRM02273.1"
                     /translation="MIRSMTGFGHGEVSNDKNQKVTVEMKSVNHRYCDISLKLPKKLA
                     MFEANIRNIMKEYASRGKIDIYVSYEDLSETAVSLHYNQAMAEEYMQVFKKMQEDFNI
                     ETKITAEALAKYPEVVTIEEVQQDEEVWWELLEAALRQAAEKFVETRTIEGANLKRDL
                     LGKLDQMAADVAFIEERSPQIIAEYRSKLEEKVKEFLEDSTIEENRIAAEVTLYADKI
                     AVDEEIVRLQSHISSMTDVLESDESIGRKLDFMAQEMNREANTILSKSSDVDLADHAI
                     ELKTNVEKVREQIQNIE"
     gene            complement(4840..>5033)
                     /locus_tag="PLC71_06240"
     CDS             complement(4840..>5033)
                     /locus_tag="PLC71_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HRM02274.1"
                     /translation="TARHVCYYMSFQEMMKYWNRAKEGGVKHFKYEELDIGFFVPLKQ
                     GMILHYLECLQKVLEQKDQ"
     assembly_gap    5034..5103
                     /estimated_length=70
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5104..5383)
                     /locus_tag="PLC71_06245"
     CDS             complement(<5104..5383)
                     /locus_tag="PLC71_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HRM02275.1"
                     /translation="MAEDKWNVKGLRGSVLEELINYTNEKYRQQKLALVQKIPTSIKP
                     VRFDKTKRHITLAYFDQKSTVDYIGVVQGIPICFDAKECAKDIFPLQNI"
     gene            complement(5386..>5844)
                     /locus_tag="PLC71_06250"
     CDS             complement(5386..>5844)
                     /locus_tag="PLC71_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HRM02276.1"
                     /translation="DMEKYYLCIVKGKLERTQKVSAYLKKDEKTNKVKVSKQEIAGAS
                     HIETAYEPVISNDEFTLLKVELITGKTHQIRAHLASIGHPLIGDFKYGDKKINQEMKQ
                     RFGLKDQLLHCTEVVFPRDMKTCRNLSGKKIQAPLPKRFDMIKNDLFGRA"
BASE COUNT         1524 a         1371 c          832 g         2047 t
ORIGIN      
        1 cacagaaaaa gagggtgatt tttcattatg aaacgggcac aaaccgaaat aagaactgcc
       61 ttttttcttc aacggtacat acgtcgaaat cagatcaaca atatcgttcc ggcttcgcac
      121 ctcttctatg atttcttcac cataaatcaa cgcttgtccc tccttaaaaa atccctctat
      181 atattattat tctacacctt tttttatttt cctttatttt tgcataaaaa atttggggat
      241 aaaaatctca gtatatttct caattgcaaa atggtctgtc attccagcaa tatagtcaca
      301 aactgccaca ggtacatctc ctggtttatc gatcatcttc ttattatatt ctggcaattg
      361 ctcaacatgt atcagataat actcatataa cggaccaatg atattcttga tcttgtcttc
      421 ttctttcttc gtatttggtt cttcataaac tctctcaaac ataaactttc gaagaccgat
      481 cattgcatct cttacttcct ttgacatcac aatatcattc agattctcac tatgttttac
      541 aatatcaagc accatcgtat tgatccgttc ccgatatgtt gttccaagga cttccgtaaa
      601 ttcttttgga agatcttctt cccgtagaac cttagcccgt acggaatcgt caatatcatg
      661 gttgatatat gcaattttgt ctgccagccg cacaattttt ccttctaatg tatgcggcat
      721 gctgtgcatt gaatgatttt tcattccatc taagacttcc catgtaagat ttaatccctg
      781 tccattcttc tccagacact gagcaaccct gacactttgt tcattatgct taaatcctgc
      841 tggattcatt tcatttaaca ctcgttctcc cgcatgtcca aacggtgtat gcccaaggtc
      901 atgtccaagc gcgatcgctt ctgtcaatgt ctcattgaga tctaatgctc ttgcgatcgt
      961 tctggcattc tgtgatactt ccaaagtgtg cattaacctt gttcgataat ggtcgcccac
     1021 tggagataaa aatacctgtg tcttatcttt taaacggcga aaagccttgc aatggacgat
     1081 ccgatcccga tccctttgga aacaggttct tatctcacat tccacctcag gcttttttct
     1141 tccttttgta cggatgcttt gtgtcgcata cggacttaat aattccatct ctgtctgttc
     1201 ttgtttttct cggatattca taagatcagc cctcccatct gcttccacaa agcgttccat
     1261 atgatatttg tttcttctat gactgtttaa tatcagcttc catcactgaa agagcatcta
     1321 gggatatttt cataacctct ttgacttcct cttctgtcat ggacagatat tttgccagtt
     1381 cttcaatctt tggttctctt ccaagctctt ttgataagaa agtagaagca tcatccagac
     1441 gatttgcctt aatcgccatc gcttctccaa cactgtcaga acctctttgc attgcgatcg
     1501 catctaaaat agaatcttct actgcactct tcgcatgtgc aaggaatcct tctgcgtctt
     1561 ttccctgata aattgccatt gcttccaaaa gaccaatatt accttcctgt acaagatctc
     1621 cgaataaaac acctttcccc gtatgtgttc ttgcaatttc aagaatctgt ggaagaaagc
     1681 tttcaatgac tgcttctcca tctcctttct ctacaagcca tgcatggatc agttcttttt
     1741 gctcttcttc tcccggaaga tccatctgtt ctaattcttc ttcatagaat cggatcgcac
     1801 cctgttcttc ttccgaaatg tcagcttttt ctttcgcctc ttcttctgca acgatctgtg
     1861 cttcatcaac acccagaacg cgaatcccag cttctacaaa acttgcgatc agaagttttt
     1921 tcgcttcttc atttaattca taatcgctaa aataatctaa taattctttg tggtcgatct
     1981 gattaccatt cgtctttgcg atctggatca tctcttccat attctttaga aataaattct
     2041 tatccatgtt ttacaaactc cttatttgat gaatgcaaaa tacacgatca ctaacagacc
     2101 gagtatgatt cggtatacac caaatgcctt aaagtcattc ttcttaatat atccaagcaa
     2161 gaatttgatc gctccgattg atacaagaaa tgatacaacc attcctacga tcagtacgat
     2221 caactgtgtt gatgtaaatg caagaccgaa cttcacgatc tttaataagc ttgctccaaa
     2281 catcacagga atccctaaga aaaatgaaaa ttcagcagct actgttctag aacatccaag
     2341 caagattcct ccaagaatcg tcgcaccaga tctagatgta cccggaatta atgccaatat
     2401 ctgaaataca ccgattccaa gtgccattcc ataggataat ctggaaagat cattcattgg
     2461 tcttcttctg cttcgtttgt tgcggttttc aataatgata aataaaacac catagataat
     2521 cagcattgct gctacaactg gtggattata aaaatgatcg ttcaaccagt catcaaacaa
     2581 aataccaata attgctgctg gaaatactgc aagaattacc tttgtccata aaatgatcgt
     2641 atccatcttc tcctcgtgat tcttcttcgg tgaaaatgga ttcaacttat ggaaatataa
     2701 gacaacgact gccatgatcg cacctaactg gataaccaca tcaaacatct ccttaaagtc
     2761 agctgtcatg ttcagcttga taaattcatc tgctaagatc aaatgtcctg tactactgat
     2821 cggtaaccat tccgtaatcc cctcaacaat tccaagtaat atggttttaa gtaacaaaat
     2881 aatgtccata aaaaactccc tgtctgtaca aataagggtg tttaggaatc cctaagcacc
     2941 ctgttttcct tactgtttct attcttctgc ttcttcgtca tctccgagaa tcttaacttt
     3001 ttcttcccaa agttcttcaa cagctgtaga taatggtttt gcaccatctt tttcttctac
     3061 taatggcata tctcctgcga tgatctgtct tgctctcttt gcacttgcaa gcacgataga
     3121 ataacggctt gagataactt tctcttcttc actgtcacca ttgatgacct tcattaattc
     3181 tgtatatgat ggatgtaaca taataattcc tcctatagct gttctaattc tttttttgtt
     3241 gcttctataa acttaagatt atttccaagc aggcattctt ttgcctgcac aatggcatga
     3301 attcgatccg cgcagtcttc tacctgatca ttgatcacga tataatcata tttatccatg
     3361 tcttctgact cttctttggc gcgatttaaa cgtttattga tcacttcctc agattctgtg
     3421 cctctgccta ctaagcgttc tttgatcgca gctgcacttg gtgctgtaat aaagatcaac
     3481 aatgcatcct catattgctt cttaatatta aatgcaccct gcacctcaat ctctaagatt
     3541 acattatgtc ctgcggcaag ttcatcttcc acaaactttc ttggcgttcc ataataattg
     3601 tccacatatc tggcatattc aataaatcca ttataatcga tcagattttt aaaatcatcc
     3661 acggtcttga aaaaatactc tctgccatca acttcacctt ctcttggttg tcttgttgtt
     3721 gcagaaattg ataaactata tccatatttt tctaccagtt tctttgccac cgttcctttt
     3781 ccaactccag aaaatccgga gattaccagt aatgaacctc tcttttccat gatctttccc
     3841 ttcttatctt actctatgtt ctgaatctgc tctcttactt tctcaacatt tgtcttcagt
     3901 tcgattgcat gatccgcaag atcgacatca cttgatttgg ataagatcgt attcgcttct
     3961 ctgttcatct cctgtgccat gaaatcaagt tttcttccga tgctttcatc gctttccaac
     4021 acatctgtca tagagctgat atgactttgc aatcttacga tttcctcatc aacagcgatc
     4081 ttatctgcat acaatgtaac ctctgctgcg atcctgtttt cttcgatcgt tgagtcttct
     4141 aaaaattctt tcactttttc ttctaatttg cttctgtatt ccgcaatgat ctgtggagaa
     4201 cgttcctcaa taaacgccac atctgctgcc atctgatcaa gtttgcctaa aagatctctc
     4261 ttaaggtttg ctccttcaat cgttcttgtc tctacaaact tctccgcagc ctgtcttaat
     4321 gcagcttcta gcaactccca ccatacttct tcatcttgct gtacttcctc aattgttaca
     4381 acctctggat atttggcgag ggcttctgca gtgatcttgg tttcaatgtt aaaatcttcc
     4441 tgcatctttt taaatacctg catatattct tctgccattg cctgattata atggagactt
     4501 accgctgtct ctgacaaatc ttcataagac acataaatat caatctttcc acggcttgcg
     4561 tattctttca tgatattacg gatatttgcc tcaaacatgg ctaatttctt gggaagtttt
     4621 aatgagatgt cacaatatct gtggttgacg gatttcatct ccactgtgac cttctgattc
     4681 ttatcattgg acacttcccc atgtccaaat cctgtcatgc ttctgatcat aaaatctatc
     4741 cttttctgca ctgcggttta aaatccacag cttttctttt tcgtcaatgt tatattatac
     4801 gcaaaatttc acttctcgtc aatattttcg acacgaatct tattgatctt tttgctctaa
     4861 tactttttgc agacattcca gataatgaag gatcattccc tgctttagtg gaacaaaaaa
     4921 accaatatct aattcttcat atttgaaatg tttcactcca ccttcttttg cacgattcca
     4981 gtacttcatc atctcctgaa atgacatata atagcaaaca tgccttgctg taannnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnngaatatt ctgaagtgga aatatgtctt ttgcacattc ttttgcatca aaacaaatag
     5161 gaattccctg cacaacccca atataatcaa cagtactctt ctgatcaaaa taagcaagcg
     5221 taatatgacg ttttgtctta tcaaatctga ctggcttgat cgatgtcggt attttctgca
     5281 caagagcaag tttctgttgc ctgtattttt cattcgtata gttgatcaac tcttcaagga
     5341 ccgatcctcg aagtcccttt acattccatt tatcttctgc catgactatg ctctcccaaa
     5401 caagtcattt ttgatcatat caaatcgctt cggaagtggt gcttgaattt tctttccaga
     5461 taggtttctg catgttttca tatctcgcgg aaaaacaacc tctgtacaat gaagcagctg
     5521 atcttttaat ccaaatctct gtttcatctc ctgattgatc tttttatcgc catatttaaa
     5581 atctccgatc aacggatgtc caatacttgc aagatgcgct cggatctgat gggtctttcc
     5641 tgtgatcagt tcgactttca atagtgtaaa ttcatcattt gatatcactg gctcataagc
     5701 tgtctcaata tggcttgctc ctgcgatctc ctgttttgat acctttacct tgtttgtttt
     5761 ttcatctttc tttaaataag cactgacttt ttgtgttctt tcaagttttc cttttacgat
     5821 gcaaagatag tatttttcca tatc
//
LOCUS       DAOMUE010000113         5748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3809_length_5748_cov_2.975935, whole genome shotgun sequence.
ACCESSION   DAOMUE010000113 DAOMUE010000000
VERSION     DAOMUE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3809_length_5748_cov_2.975935"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3147)
                     /gene="ileS"
                     /locus_tag="PLC71_06255"
     CDS             complement(<1..3147)
                     /gene="ileS"
                     /locus_tag="PLC71_06255"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRM02277.1"
                     /translation="MYKKVSSDMNFVDREKETVKFWKENEIFEKSIEERKDDPTYTFY
                     DGPPTANGKPHIGHVLTRVIKDMIPRYQTMKGHKIIRKAGWDTHGLPVELEVEKMLGL
                     DGKEQIEEYGMEPFIKKCKESVWKYKGMWEDFSDTVGFWADMENPYITYDNNFIESVW
                     WALKQIWDKGLLYKGFKIVPYCPRCGTPLSAQEVAQGYKDVKERSAIVRFKLVDEDAY
                     FLAWTTTPWTLPSNIGLCVNPEETYAKVKAADGYVYYMAEALLDTVLGRLADEEAGTP
                     AYEVLETYKGKDLEYKEYEPLYDCAAKVAEKQHKKAFYVTCDGYVTLTDGTGIVHIAP
                     AFGEDDAKVGRKYEMPFVQLVDERGNMSEETPFAGLFVKKADPEVLKDLESRGLLYDA
                     PKFEHSYPHCWRCDTPLIYYARESWFIKMTDVKDQLIANNNTVNWVPKSIGKGRFGDW
                     LENVQDWGISRNRYWGTPLNIWECECGHQHSIGSIEELKSMSDNCPDDIELHRPYIDQ
                     VTIKCPHCGKPMHRVEEVIDCWFDSGSMPFAQWHYPFENKEIFEDNFPANFISEAVDQ
                     TRGWFYSLMAISTLLFNKAPYKNVVVLGHVQDENGQKMSKSKGNAVDPFDALEEHGAD
                     AIRWYFYTNSAPWLPNRFHAKAVTEGQRKFMGTLWNTYAFYTLYAEIDQFDPTKHALE
                     YDKLSVMDKWLLSKMNTMVKSVDDNLGNYRIPEAARALQEFVDDMSNWYVRRCRDRFW
                     AKGMEQDKVNAYMTLYTALVTVCKAAAPMIPFMTEEIYQNIVRSVDASAKESIHLCDF
                     PVYNAEQVDEKLEADMDLVLKIVVLGRACRNGAAIKNRQPIGQMYVKAEASLPDYDEA
                     IILDELNVKNITFTDDVSNFTTYNFKPQLKTVGPKYGKLVGGIRNYLAAVDGNEAKAQ
                     LDSKGVLTFEVDGTPVELAEEDLLIEMVKQEGFVTEADNGVTVVLDTNLSEELLEEGF
                     VREVVSKIQSMRKEAGFEVTDHITFSYKGSDKAADIIGRNEAAIAGDVLADEVKAGEV
                     AGYEKEWNINGEKVTLGVE"
     gene            complement(3498..4331)
                     /locus_tag="PLC71_06260"
     CDS             complement(3498..4331)
                     /locus_tag="PLC71_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRM02278.1"
                     /translation="MKQMLVNSKKMLEEAKIGHYAVPAANIVNLENIKGVIEAAEETK
                     HPLMVCLAEVHTPTLGIEEAAAIVKYYAEKSNQDIALHYDHGFTIELVKKAIDVGFSS
                     VMIDGSSLLFEENVAKTKEIVAYAHERNVTVEAEIGHVGEGDSYHVEGNTMLTTPEEA
                     KKFVEQTGVDSLAVSIGTAHGEYKGIPHLNFERLQEIATEVSIPLVLHGGSGSGDENL
                     SKAVELGIAKVNICTDLLNAARDNTKDGYAERSYYDSVTLAKDGFKDCLKHYYEVFHT
                     K"
     gene            complement(4386..5642)
                     /locus_tag="PLC71_06265"
     CDS             complement(4386..5642)
                     /locus_tag="PLC71_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRM02279.1"
                     /translation="MKTRAVRLYGESDLRLEEFDMGELKDDEILMELITDSVCMSTYK
                     ESIQGAKHQRVNDDISEHPIIVGHEFAGIIREVGAKWKDKYQVGTKYTMQPAINIKGS
                     MAAIGYSYEYCGGAATFIKVPPIVMEKDCLLPFDENSAFFEASLAEPMSCIIGAFHSM
                     YHSERGVYEHKMGIVEGGKSALLASCGPMGLGAISYMLNCNRKPSLLVITDIDEVRLK
                     RASELFTEEYAKERGVEIHFVNTAKVDDPVKTLRDLTGGTGFDDVSVYAPVRPVIEMA
                     DEILGFDGCLNFFAGPVDPKLSAMFNFFDVHYKMHHLVGSSGGNTDDMKECLKLAGEG
                     RLDPAVMITHIGGIDAQIDTILNLPKIPGGKKLMYLGKNLELTAIEDFEEKGKTDDRF
                     KELAKITKEHNGLWSKEAEDYLLANF"
BASE COUNT         1432 a         1296 c          936 g         2084 t
ORIGIN      
        1 ttcaacacct aaagtaactt tctcaccatt gatgttccat tctttttcat atccagcaac
       61 ttctccggct ttcacttcat ctgctaaaac gtctccagcg atcgctgctt cgtttcttcc
      121 gatgatgtct gcggctttgt ctgatccttt gtaagagaat gtgatatgat ctgttacttc
      181 gaatcctgct tcttttctca tagactgaat cttgctgact acttcacgga cgaatccttc
      241 ttctaacagt tcttcggata agtttgtatc aagtacaact gtcactccgt tgtctgcttc
      301 tgttacgaat ccttcctgtt ttaccatttc gatcaacaga tcttcttctg ctaattctac
      361 tggtgttcca tcaacttcaa atgttaatac tcccttagag tctaactgag ctttcgcttc
      421 atttccatca actgctgcta agtagtttct gattcctcca actaatttgc cgtattttgg
      481 tcctacagtc tttaactgtg gtttgaagtt gtatgttgtg aagtttgata cgtcatctgt
      541 aaatgtgata ttcttcacat ttaactcgtc taagatgatc gcttcgtcat aatctggcag
      601 agatgcttct gcttttacat acatctgtcc gattggctga cggttcttga tcgctgctcc
      661 attacggcat gcacgtccta atacgacgat ctttaagacg agatccatat ctgcttctaa
      721 tttctcatct acctgttctg cattgtatac agggaagtcg cataagtgga tactttcttt
      781 tgcagatgca tcaacacttc ttacgatgtt ctggtaaatc tcttctgtca tgaatgggat
      841 cattggagct gctgctttac atacagtaac taatgctgtg tataaagtca tatatgcatt
      901 taccttatcc tgttccattc cttttgccca gaaacggtca cggcagcgtc ttacatacca
      961 gttactcata tcatctacaa attcctgtaa tgctcttgca gcttctggaa ttctgtagtt
     1021 tcctaagtta tcatccacag atttgaccat tgtgttcatc ttagataaca gccatttatc
     1081 cattacagat aacttgtcat attctaacgc atgttttgtt ggatcaaact ggtcgatctc
     1141 agcgtataat gtgtagaatg catacgtatt ccataatgtt cccatgaatt tacgctgtcc
     1201 ttctgtaacg gcttttgcat ggaaacggtt tggcagccat ggggcactat ttgtatagaa
     1261 ataccaacgg atcgcatctg ctccatgctc ttctaatgca tcgaatggat caactgcatt
     1321 tcctttggac ttactcatct tctgtccgtt ttcatcctga acgtgtccta atacgacaac
     1381 gtttttatat ggtgctttat taaataataa agttgaaatt gccattaagg agtagaacca
     1441 tccacgagtc tggtctacag cttctgagat aaagtttgct gggaagttat cttcgaagat
     1501 ttctttattc tcaaatgggt aatgccactg tgcgaaaggc atagatcctg aatcgaacca
     1561 gcagtcgatt acttcttcta cacgatgcat tggttttcca caatgtggac acttaattgt
     1621 tacctggtcg atatatggac ggtgaagttc aatatcgtct ggacagttat cagacatact
     1681 ctttaattct tcgatagaac cgattgaatg ctgatgtcca cattcacatt cccagatatt
     1741 taatggagtt ccccaataac ggtttcttga gattccccaa tcctgtacgt tctctaacca
     1801 gtctccaaaa cgtcctttac cgatgctctt tggtacccag ttaactgtat tgttatttgc
     1861 gatcagctga tcttttacat ctgtcatctt gatgaaccaa gattctcttg catagtagat
     1921 cagtggtgta tcacaacgcc agcagtgtgg atagctatgc tcgaattttg gagcatcata
     1981 taataatcca cggctctcta aatcttttaa gacttctgga tctgctttct taacaaataa
     2041 tccggcaaat ggtgtttctt ctgacatatt accgcgttcg tctactaact gtacaaaagg
     2101 catctcatat ttgcgtccga ctttcgcatc gtcttcacca aatgctggtg cgatatgaac
     2161 gatacctgta ccatctgtta atgttacata tccatcacat gttacataga atgctttctt
     2221 gtgctgtttc tctgcaactt ttgctgcaca gtcatataat ggttcgtatt ctttgtattc
     2281 taagtctttt cctttgtatg tttctaagac ttcatatgca ggtgttcctg cttcttcgtc
     2341 tgctaatctt ccaagaactg tatcaagcag tgcttctgcc atatagtata catatccatc
     2401 tgcggctttt acttttgcgt atgtttcttc tgggtttacg caaagaccga tattagatgg
     2461 aagtgtccat ggtgttgttg tccatgcaag gaaatatgca tcttcatcta ctaatttaaa
     2521 gcgaacgatc gctgaacgtt ctttgacatc tttgtatccc tgtgccactt cctgtgctga
     2581 taatggtgtt ccacaacgtg ggcagtaagg tacgatctta aatcctttgt aaagtaatcc
     2641 tttatcccag atctgtttta atgcccacca tacagattcg ataaagttat tatcgtatgt
     2701 gatgtatggg ttctccatat cagcccagaa acctactgta tctgagaaat cttcccacat
     2761 acctttgtat ttccaaacgc tttctttaca tttcttgatg aatggttcca taccgtattc
     2821 ttcgatctgt tcttttccat caagacctaa catcttctca acttcaagtt cgactggaag
     2881 tccgtgtgta tcccatccag ctttacgaat gatcttatga cctttcattg tctggtaacg
     2941 agggatcata tctttgataa cacgagttaa aacgtgtccg atatgtggtt taccattggc
     3001 tgttggtggt ccgtcataga atgtatatgt cggatcgtct tttctttctt cgatactctt
     3061 ttcaaaaatt tcgttttctt tccagaactt tacggtctct ttctctcgat cgacaaagtt
     3121 catgtcagat gatactttct tgtacatctt catctctcct tttacttctt agaaaatcta
     3181 atgaaatagg aaaaaataag aaaaagcgct gtccaaaata ggacaacgct gttgcttcta
     3241 aaccacctat ttcacatact atcatatgtt atcccgttaa cggaggatac cggcacagct
     3301 tacttcattc agcctgcagc ttgggagtga tcttccttct tatcttacta acacccggct
     3361 tccaccgccc cggactcgct catgcgttca tgatagaagt actgtctcct tcttcgcttt
     3421 tatcatacta agttatctta acacttgaaa tggcggttcg tcaatattga attgtatgtt
     3481 ttcgcatttc tttttgttta ttttgtatgg aatacttcgt aataatgttt caggcaatct
     3541 ttaaatccat cttttgcaag tgttacacta tcatagtaac ttctttcggc atatccatct
     3601 tttgtattat ctcttgctgc atttaataag tctgtacaga tatttacttt cgcaattcca
     3661 agttctactg ctttggataa gttttcatct ccagaaccag atcctccatg aagtactagt
     3721 ggaatagaaa cctcggttgc aatttcttgt aatctttcaa aattcagatg tggaattcct
     3781 ttgtattctc catgagctgt tccaatagat actgcaaggg aatcaactcc tgtttgttct
     3841 acaaatttct ttgcttcttc tggtgttgtt aacattgtat ttccttcaac atgatagctg
     3901 tctccttctc ctacgtgtcc gatttcagct tctaccgtaa catttctttc atgtgcatat
     3961 gctacgattt cttttgtctt tgccacattt tcttcaaata gaaggcttga tccatcaatc
     4021 ataacggagg aaaatccgac atcaatagct ttcttaacca actcgatcgt aaatccatga
     4081 tcataatgaa gcgcgatatc ttggttactt ttttctgcgt aatattttac gatcgctgct
     4141 gcttcttcaa tacctaatgt cggtgtatgt acctccgcca gacacaccat caatgggtgt
     4201 tttgtttctt ctgccgcctc gatcactcct ttgatattct caaggtttac aatgttggct
     4261 gctgggactg cgtaatgtcc tattttggct tcttctaaca tctttttact gtttactaac
     4321 atctgtttca tcctcccgaa aattcattct aaaattttac tttataagtt ccacttgtat
     4381 catttttaaa aattagctaa cagataatct tctgcttctt tggaccataa tccattatgt
     4441 tcttttgtaa tcttagctaa ttctttaaat ctatcatctg tttttccttt ttcttcaaaa
     4501 tcttcaattg ctgttagttc aagattcttt ccaagataca tcagtttctt tcctcctgga
     4561 atctttggaa gattcaatat cgtatcgatc tgtgcatcaa ttcccccgat atgcgtgatc
     4621 ataacagctg gatctaatct tccttctcct gcaagtttta aacattcttt catatcatcg
     4681 gtattcccac cgcttgatcc taccagatgg tgcatcttat aatgtacgtc aaagaaatta
     4741 aacatcgcag ataattttgg atccactggt cctgcgaaga agtttaagca tccgtcaaat
     4801 cctaaaatct catctgccat ctcgatcact ggtctgactg gtgcatatac agatacatca
     4861 tcaaatcctg ttccaccagt taaatctctt aatgtcttca ctgggtcatc gactttcgca
     4921 gtattgacaa aatggatttc aacacctcgt tctttggcat attcttctgt aaataattca
     4981 ctagctcttt ttaatcttac ttcatcaatg tctgtgatca ccagtaaaga aggtttacga
     5041 ttacaattta acatataact gattgctcct aatcccattg gaccgcagct tgcaagaagt
     5101 gcactctttc caccttctac aattcccatc ttatgttcat aaacaccacg ttcactgtgg
     5161 tacatagaat ggaatgctcc aatgatacag ctcataggct ccgccagtga tgcctcaaaa
     5221 aatgcactgt tttcatcaaa aggtaataag cagtcttttt ccattacaat tggcggaact
     5281 ttgataaagg ttgctgctcc tccgcagtat tcataagaat atccgatggc tgccatagaa
     5341 cctttgatat tgatcgctgg ctgcatggta tattttgttc caacttgata tttatctttc
     5401 cacttcgccc caacttcacg aatgattcct gcaaattcat gtcctacgat gatcggatgt
     5461 tcgctgatat catcgttgac tctctgatgt tttgctccct gaatagattc tttgtaagta
     5521 gacatgcaga cactgtcagt gatcagttcc attaagatct catcgtcttt cagttctccc
     5581 atatcaaatt cttctaatct taaatctgat tccccataaa gtctgactgc tcttgtcttc
     5641 atagcttatc ccttttcctt tctgtttgcg tcgtgtggtc atatgaccat gtacattgtc
     5701 atttgatatt tttttattat agattcaacg tttttatttg tcaataca
//
LOCUS       DAOMUE010000114         5748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3810_length_5748_cov_2.623222, whole genome shotgun sequence.
ACCESSION   DAOMUE010000114 DAOMUE010000000
VERSION     DAOMUE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3810_length_5748_cov_2.623222"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLC71_06270"
     CDS             complement(<1..208)
                     /locus_tag="PLC71_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02280.1"
                     /translation="MKNTAIEKLSYTEVTKNISQAEYNQTQEEYILSKSPDKYMISLP
                     VKVLMFEGAKDSSPLTYYFYMENDE"
     gene            355..1671
                     /locus_tag="PLC71_06275"
     CDS             355..1671
                     /locus_tag="PLC71_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRM02281.1"
                     /translation="MDLFEYARELNKDKESPLAGRMRPATLDEVVGQEHIIGKDKLLY
                     RAIKADKISSIIFYGPPGTGKTTLAKVIANTTKANFVQMNATTSGKKDMQEAVADAKE
                     SLGMYQKKTILFIDEIHRFNKAQQDYLLPFVEDGTIILIGATTENPYFEVNQALISRS
                     NVFELHSLDKEDIKKLIVRAITDDEKGMGIYGATITDDALDFLSDMAEGDARSALNAI
                     ELGILTTEPAEDGKIHIDIDVAQECIQRRVTKYDKDGDNHYDVISAFIKSMRGSDPDA
                     AIYYLARMIDAGESVTFIARRIMICASEDVGNADPQALQVAVAASQAVERIGMPEARI
                     ILAQAVTYVASAPKSNAAYMAVDQALESVRNHKIGAVPNHLRDAHYKGAAKLGHGIGY
                     KYAHDYPDHYVKQQYLPDELLGTTFYEPTENGYEKNIKEYLERIRK"
     gene            complement(1700..1903)
                     /locus_tag="PLC71_06280"
     CDS             complement(1700..1903)
                     /locus_tag="PLC71_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02282.1"
                     /translation="MKKQKRMKQITAILLIVILLAIIGSTIYFAVSGSQLFWMSLFLM
                     FFFPIFLWAMSFLYKWSKNKNEH"
     gene            2214..2720
                     /locus_tag="PLC71_06285"
     CDS             2214..2720
                     /locus_tag="PLC71_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRM02283.1"
                     /translation="MALLQEWRDYAYKFDDRTAEGQQFWLNYFNIEKGIYEQLLADKD
                     TVVSGTVKELADKYETELQMFVGFLDGINDSLKEANPIEEMDENTQVSLDIDFEKLYY
                     NMVGCRAEWLYNLKEWDDILTEERRKELYKESRNSTTVVKGKKIGRNDPCPCGSGKKY
                     KKCCGKNA"
     gene            2945..3412
                     /locus_tag="PLC71_06290"
     CDS             2945..3412
                     /locus_tag="PLC71_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009264708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRM02284.1"
                     /translation="MKKCEGIYSLIGKVNHKSVIYGFELLKEENIHPRQMPLIIHLKK
                     CEGCTQKELAEKMQVKPSTLNVMIGRMEKNGYIEKKQDEKDSRKSRIYFTEKGRSICE
                     DGHKKFVMIQEKLQEYFTKEEQEELKRLLNKFCDCLDEQTEILKKERKEKSDA"
     gene            3405..5594
                     /locus_tag="PLC71_06295"
     CDS             3405..5594
                     /locus_tag="PLC71_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM02285.1"
                     /translation="MLKLFRYLKKAYVPIIAIVLLLILQASCDLTLPTFTSNIVNVGI
                     QQKGIEDAVPDVMREETFLALKSLMKQDDADDMEDAYKLYTKDQVKDSKYKDYKDGRL
                     YVRRYISKKDREHLDTSMSKAMLKLSAQMAKQIQANPQAAASLSKSQKKMMAQMKNMD
                     TKDMPDTIISQAAISFVTSEYKAIGLDIDQMQTHYLLVTGAKMIGLAFLIMAAAVSVT
                     LLSARLAAKLSRILREKVFEKVMSFTNSEFDKFSTASLITRSTNDIQQIQMFMTMVFR
                     IVVYAPLMGIGGIFKVLTTNAKMTWTIAIGVIAIMLVIFVLFKVAMPKFKILQKLIDR
                     LNLVTREILTGLSVIRAFSTEKHEEERFDKANMDLMKTNLFVNRAMTFMMPTMMLIMN
                     GLTVLIVYAGASNIDAGKMQVGDLMAFIQYAMQIIMAFLFISMVSIMMPRAQVAAERV
                     NEILDMEVMIKDPEEPKEFLPEKKGEVEFKNVYFCYPDADEAVLHNISFTAPAGKTTA
                     FIGSTGSGKSTLINLIPRFFDVSRGSILVDGVDIRDVKQHDLCEKIGYVPQKGVLFSG
                     TIESNLKYGKEDATIDEVKRAARIAQATDFIEEKEEKYDSPIAQGGSNVSGGQKQRLS
                     IARAIAKDPEIYIFDDSFSALDYKTDVKLRSELQKETNGSTTLIVAQRISTILHADQI
                     IVLDEGNIVGKGTHEELLNTCPVYQQIAKSQLSEDDLKKAREVSDHE"
BASE COUNT         2114 a          885 c         1227 g         1522 t
ORIGIN      
        1 cctcgtcatt ttccatataa aaataatatg taagtgggga actgtccttt gctccttcaa
       61 acattaatac cttgaccgga agactgatca tatatttatc aggagatttg gacaggatat
      121 attcttcctg tgtctggtta tattcagctt gactgatatt tttcgtaact tcggtataag
      181 aaagtttttc aattgctgta tttttcatga taactcctaa cccgtgcttt gttattaact
      241 tctttcatta tagcaaagga atatgataaa ttcatgtctt atttataaaa aatgtatgaa
      301 taattcatag attttctcaa taccctgtgc taagataaaa tctggaggaa aactatggat
      361 ttatttgaat atgcaagaga attaaataaa gataaagagt cgccattagc agggagaatg
      421 agacctgcaa ccctcgatga agtggtgggt caggaacata ttattggaaa agataaatta
      481 ctatatagag cgatcaaagc ggataagatc agctcgatca ttttctatgg tccaccagga
      541 acaggaaaga caaccttagc aaaagtcatt gccaatacga ccaaagcaaa ttttgtacag
      601 atgaatgcaa caacatctgg gaagaaagat atgcaggaag cagtggcaga tgcgaaagaa
      661 tctcttggaa tgtatcagaa gaagacgatc ttgtttatcg atgagatcca cagattcaat
      721 aaagcacaac aagattacct gctgccattt gtagaagatg gaacgatcat tttgatcgga
      781 gcaacgacag agaatccgta ttttgaagta aatcaggcgt tgatctcaag atccaatgta
      841 tttgaactac attcattaga taaagaagat attaagaaat tgatcgtaag agctattaca
      901 gatgacgaaa aaggaatggg aatttatgga gcaacgatca ccgatgatgc actggatttt
      961 ctctcagata tggcagaagg agatgcaaga agtgctttaa atgcgatcga acttggaata
     1021 ttaacaacag aacctgctga agatggaaag attcatatcg atatcgatgt agcacaggaa
     1081 tgtatccaaa gaagagtgac aaaatacgac aaagacgggg ataatcacta tgatgtgatt
     1141 tctgcattta tcaaaagtat gcgaggatca gatccagatg cagctattta ttatctggca
     1201 agaatgatcg atgccggaga aagtgttaca tttattgcaa gaagaatcat gatctgtgca
     1261 tccgaagatg ttggaaatgc tgatccgcag gcattacagg tagcagttgc agcgtcacag
     1321 gcagtggaac ggatcggaat gccagaagca aggatcatac tggcacaggc agtcacttat
     1381 gtagccagtg caccaaagag caatgcagca tatatggcag ttgatcaggc attagaatct
     1441 gtcagaaatc ataagatcgg agcagttcct aatcatttaa gagatgctca ttacaaaggc
     1501 gcagcgaaac taggacatgg aatcggatat aaatatgcgc atgattatcc agatcattat
     1561 gtaaaacaac agtatcttcc agatgagctt cttggaacaa cgttttatga accaacggaa
     1621 aatggatatg agaaaaatat caaagaatat ttggaaagaa tcagaaagta aaataaaaat
     1681 gagcactgaa aatgaaacat cagtgctcgt tcttattttt actccattta tacaaaaatg
     1741 acattgccca taaaaagatc ggaaagaaaa acatcaaaaa cagtgacatc cagaataact
     1801 ggcttccgct cactgcaaaa tagatagtag atccgatgat ggcaagcaat atcacgatca
     1861 gcaggatcgc tgtgatctgc ttcatacgct tttgtttttt catcataaca tcctttcgta
     1921 aatatgtata cctatgaggt atcctagcat agattattgt gttttgaaag agtaaaatat
     1981 tttataaaag aaaatgaata agaagcaaaa gagaggacat aataagaata tccagttaat
     2041 aaaagaactg gatagagaaa tacttatcct ccccttaagt attcccccac tctgcgaagc
     2101 cgtggaatgg gggattttta tgtaaaattg ctgtcgattg ttgaaaaaga gccagagatt
     2161 tgttatagta tcaaaggaaa ataacatttt taaaagaaaa ggatggaatg aatatggctt
     2221 tattacagga atggcgtgat tacgcatata aatttgacga tagaacagca gaaggacagc
     2281 agttctggtt aaattacttc aacattgaaa aaggaatcta cgagcaatta ttagctgaca
     2341 aagatacagt tgtatctgga acagtgaaag aattagcaga caaatacgag acagaattac
     2401 agatgtttgt aggattctta gatgggatca acgacagctt aaaagaagca aacccaatcg
     2461 aagagatgga tgaaaataca caggtaagct tagacatcga cttcgagaaa ttatattaca
     2521 acatggttgg atgtcgtgca gaatggcttt acaacttaaa agaatgggat gacattctta
     2581 cagaagaaag aagaaaagag ttatacaaag aatccagaaa ttcaacaaca gttgtaaaag
     2641 gaaagaagat cggaagaaac gatccatgcc catgcggaag cggtaaaaaa tacaaaaaat
     2701 gctgcggaaa aaacgcttaa ttcgttaaaa gaaatagtcg aatatgtaaa tgaaacaagg
     2761 aagaaccttt tggcttatgt gatagctgaa gggttctttt attttagaaa attttgataa
     2821 aatcccatat gagataaaaa tacagaaatt attgacattt tgtatacatg atgttagaat
     2881 ttcaattagt cgtctaatga ttagttatct aattaaaata ctcaaagtaa agaaaggagc
     2941 atacatgaag aagtgtgaag gaatttacag tttgatcggc aaagtgaatc ataagagtgt
     3001 gatttatgga tttgagcttt tgaaagaaga aaatatacat ccgagacaga tgcctctgat
     3061 cattcattta aaaaaatgcg aaggctgtac acaaaaagag ttagccgaga agatgcaggt
     3121 caagccgtct acgctaaacg taatgattgg acgaatggaa aagaatggat atatagagaa
     3181 aaaacaagac gaaaaagatt caagaaaaag cagaatttat tttactgaga agggaagaag
     3241 tatctgtgaa gacggtcata aaaaatttgt catgatacaa gaaaaacttc aagagtattt
     3301 tacaaaagag gagcaggaag aactcaagag actgttaaat aaattttgtg attgtctgga
     3361 tgaacagaca gagatactaa aaaaagaaag aaaggaaaaa tcagatgctt aaattatttc
     3421 gatatttgaa aaaagcatat gtgcctatca ttgcgatcgt gttattgctg atcctacagg
     3481 cgagttgtga cctgacatta ccaacattta catccaatat cgtcaatgta ggtattcagc
     3541 aaaagggtat tgaagacgca gttcctgatg taatgagaga agaaacattt ctggcattga
     3601 aatctcttat gaaacaagat gatgcagatg atatggaaga tgcttataaa ctttatacaa
     3661 aagatcaggt aaaagattca aaatataaag attataaaga cggacgttta tatgtcagaa
     3721 gatatatctc caaaaaagac agagaacatc ttgatacgag tatgtcaaag gcaatgctta
     3781 agttgtcagc gcagatggca aaacagattc aggccaatcc tcaggcagca gcttctttaa
     3841 gcaagagcca gaaaaaaatg atggcgcaga tgaagaacat ggatacaaaa gacatgccag
     3901 atacgatcat cagtcaggca gcgatttctt ttgtgacttc agaatacaaa gcgatcggac
     3961 ttgatattga ccagatgcag acacattatc tgttagtgac aggagcaaag atgatcgggc
     4021 ttgcgttctt gatcatggca gcagcagtga gtgttacttt attgtcagca cgtttagctg
     4081 cgaaattaag tcgtattctg agagagaaag tatttgagaa agttatgtcg tttacaaact
     4141 ctgagtttga taaattctca acagcctcat tgatcacaag aagtacgaat gatatccagc
     4201 agattcagat gtttatgacg atggtattcc gtatcgtcgt atacgcacca ttaatgggaa
     4261 tcggaggtat tttcaaagta ttaacaacca atgcgaagat gacatggacg atcgctatcg
     4321 gtgtgatcgc gatcatgtta gtgatctttg tattatttaa ggtagcgatg ccaaaattta
     4381 agatcttaca gaaactgatc gaccgattaa acttagtaac aagggaaatt cttactggac
     4441 tttccgtaat ccgtgcattc agtacagaaa aacatgaaga agaacgtttt gataaagcca
     4501 atatggatct gatgaagaca aatttatttg taaacagagc tatgacattt atgatgccaa
     4561 caatgatgct gatcatgaat ggtttaactg ttctgatcgt atatgctgga gcatccaata
     4621 ttgatgcagg aaagatgcag gttggagatc tgatggcatt tatccagtat gcaatgcaga
     4681 tcattatggc attcttattt atctccatgg tatctattat gatgcctcgt gcacaggttg
     4741 cagcagaacg tgtcaatgag atcttagata tggaagtcat gatcaaagat ccagaagaac
     4801 caaaagaatt tttaccagag aaaaaaggtg aagttgaatt taaaaatgta tatttctgct
     4861 atcctgatgc agatgaagca gtgcttcata atatcagctt tacagcacca gctggtaaaa
     4921 caaccgcatt tattggaagt acaggtagtg gtaaatcaac attgatcaac ctgattccta
     4981 gattctttga tgtcagccgt ggaagtattt tggttgatgg ggtggatatc cgtgatgtaa
     5041 aacagcatga tttatgtgaa aagatcggat acgtaccaca gaaaggagta ttgttctcag
     5101 gaacgatcga atccaactta aaatatggaa aagaagatgc aacgatcgat gaagtgaaac
     5161 gtgcagcaag aatcgcacag gcaacagatt tcattgagga gaaagaggaa aagtatgatt
     5221 caccgatcgc acagggcggt tccaacgtat ctggtggaca gaaacagaga ttatcaattg
     5281 caagagctat tgcaaaagat cctgaaatct atatttttga tgacagtttc tcagcacttg
     5341 attataagac agatgtgaaa cttcgttctg aacttcagaa agaaacaaat ggaagtacaa
     5401 cactgatcgt agcacagagg atcagtacga tccttcatgc agaccagatc atcgtcttag
     5461 atgaaggaaa tatcgtagga aaaggaactc atgaagagtt attaaatact tgtccggttt
     5521 atcagcagat tgccaaatcg caattatcag aagatgatct taagaaagca agggaggtgt
     5581 ctgaccatga gtaataatcg aagaagaggt ccgatgggtg gaccaggcag aggaatggca
     5641 ccaggcgaga aagccaaaga tttaaaagga acaatggtca aactgatcaa atatatgaga
     5701 cgtttctatg ttccgatcgt tatggtaatc atttttgcca ttgcaagt
//
LOCUS       DAOMUE010000115         5653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3903_length_5653_cov_2.407646, whole genome shotgun sequence.
ACCESSION   DAOMUE010000115 DAOMUE010000000
VERSION     DAOMUE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3903_length_5653_cov_2.407646"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..663)
                     /gene="nrdG"
                     /locus_tag="PLC71_06300"
     CDS             complement(175..663)
                     /gene="nrdG"
                     /locus_tag="PLC71_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRM02286.1"
                     /translation="MHYGEIKKCDIANGPGVRVSLFVSGCRNHCPGCFNKETWDFCYG
                     KPFTTETKDHIMELLKPDYIEGFSLLGGEPFEPENQEELVRLLKEIKENYPKKNIWCY
                     TGYTYDKDLLEGGKVYTPFTEEMLSYIDTLVDGQFIESQKDITLKFRGSANQRILDLN
                     EK"
     gene            complement(687..2876)
                     /gene="nrdD"
                     /locus_tag="PLC71_06305"
     CDS             complement(687..2876)
                     /gene="nrdD"
                     /locus_tag="PLC71_06305"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRM02287.1"
                     /translation="MNGDVKTKIIKRNGEEVTFDLDKIINAITKANEEVTKIHQMNQY
                     QIMAIAEKVADQVANSSHAVNVEDIQNMVETGIMEMRGYEVAQKYVRYRFKRELRRKS
                     NTTDDGILSLLDNINEEVNQENSNKNPVINSTQRDYMAGEVSKDLSKRVLLPNEIIRA
                     HEEGIIHFHDSDYFAQREHNCDLINLEDMLQNGTVISETLIEKPHSFFTACNVTTQIV
                     AQVASNQYGGQSFTLAHLAPFVDISRKKLRKNVVKERKVIGESMDDAIIDKITEMRLY
                     DEIKQGIQTIQYQLVTLMTCNGQAPFVTVFMYLDEVPEGQTRDDLALVIEEVLKQRIQ
                     GVKNEKGVWITPAFPKLIYALDDDNITPDSKYWHLTELAAKCTAKRMVPDYISAKVMR
                     ELKNGEVYPCMGCRSFLTVEDSQRNADGSHKFYGRFNQGVVTINLVDVACSSNGDMDK
                     FWDILEERLELCHRALRCRHERLKGAVSDVAPILWQHGALARLKKGEKIDKLLYDGYS
                     TISLGYAGLYEMCVRMTGKSHTSDEGKEFALKVMQKLNDKCNEWKAAENISYSVYGTP
                     MESTTYKFAKCLQKRFGIIPGVTDKNYITNSYHVHVTEEIDAFSKLAFESEFQKLSPG
                     GAISYIEVPNMQDNIPAVLSVMKFIYNNIMYAELNTKSDYCECCGYDGEIQIKEDESG
                     KLIWECPNCGNTDQDHMFVARRTCGYIGTQFWNQGRTQEIKDRVLHL"
     gene            complement(3036..3596)
                     /locus_tag="PLC71_06310"
     CDS             complement(3036..3596)
                     /locus_tag="PLC71_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRM02288.1"
                     /translation="MDLLTLLTLAVGLAMDAFAVSICKGLAMREKVLKKGIIVGLWFG
                     GFQALMPTIGFFLGTQFKDQITSIDHWIAFVLLGLIGINMVKEALSNDEEQADDSIAV
                     KEMFMLAVATSIDALAVGITFAFLNVHIVSAASMIGVCTFLISFVGVKIGNIFGTKYK
                     SKAELAGGIILILLGFKILFEHLHIL"
     gene            complement(3877..4356)
                     /locus_tag="PLC71_06315"
     CDS             complement(3877..4356)
                     /locus_tag="PLC71_06315"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRM02289.1"
                     /translation="MRYYYDYSFPIGNLTIEEDGHGICGIYFGTKTLEGEKKNTELIR
                     EAALQLSQYFDGRRKKFDLKLSYHGTEFQIKVWKALEKIPYGETYTYKDIAESVGIPK
                     GCRAVGMANNRNPIVIVVPCHRVIGMNGSLTGYAGGLEMKEYLLDLEKRNQKLKERY"
     gene            4495..>5653
                     /locus_tag="PLC71_06320"
     CDS             4495..>5653
                     /locus_tag="PLC71_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRM02290.1"
                     /translation="MDPSMILQIIAIIILLMLSAYFSSAETALTTVNPHSMRAMAEDG
                     NKKAAIVLKLIENPSQMLSSVLVGNNLVNISLTSLTTTIAIRIFGSMGAGIATAVITV
                     LVLIFGEITPKTFATINNTKLALSYASSIYLITKLLTPVVYIINKLSTAILIILRIDS
                     KQSKRIMTERELRTIVDVSHEEGVIEESEKDMLNNVFDFKESLAKDIMIPRIDVTFVS
                     VDASYDEVLDIFREAGYSRLPVYEGSKDHVVGIVYFKDVYFYRSQHRGEIFHLKDVLR
                     KPFFTYETQKVSSLLAQMREKSVSFSIVLDEYGVTAGLITLEDIVEEIFGELRDEYDD
                     SEEETFKKLSDGSYMVSASLKLDDLDDLLDVDLDSEDYDSLGGYIIELLDHL"
BASE COUNT         1569 a         1255 c          920 g         1909 t
ORIGIN      
        1 attgaaatca atctccagta tcattgtgca acccttcttt cctctttcga aatttacaac
       61 ttatctatga tattttgtta tacatagact ataatataaa gcacagtctg tcaagagctt
      121 ctcttccaga tgataaagaa aaagatgcag tgtaaaaaac tacatctctc tcgtctattt
      181 ttcattcaga tctaaaattc tttgatttgc acttcctcgg aattttaatg tgatatcttt
      241 ctgggattcg ataaactgtc catcaaccag cgtatcaata taagataaca tctcttccgt
      301 aaacggtgtg tatactttac caccttcgag caaatctttg tcatacgtat atcctgtata
      361 gcaccagata ttcttctttg gataattttc tttgatctct tttaataatc taaccagttc
      421 ttcctgattc tccggttcaa atggttcccc tccaagaagc gaaaaacctt caatataatc
      481 tggctttaac aattccatga tatgatcttt tgtctccgtc gtaaatggct ttccataaca
      541 aaagtcccat gtctccttat taaagcatcc tggacaatga tttcggcagc ctgatacgaa
      601 taagctgact ctgactcctg gtccattcgc aatatcacat tttttaattt ctccgtagtg
      661 catatgatct cctctgtcat cgtgtcttat aaatgaagga ctcgatcctt gatttcctgt
      721 gtacgtccct gattccagaa ctgtgttccg atatatccac aagtacgtct tgcaacaaac
      781 atatgatcct gatctgtatt tccacagttt ggacattccc agatcagttt tcctgattca
      841 tcttctttga tctgaatctc tccgtcgtat ccacaacatt cacagtaatc actctttgta
      901 tttaactctg catacatgat gttattgtaa ataaatttca tcacggataa gacggctgga
      961 atattatcct gcatgtttgg tacttcaatg tagctgatcg ctccgcctgg agataatttc
     1021 tggaattctg actcaaatgc cagtttgctg aatgcatcga tctcttctgt tacatgaaca
     1081 tgatagctgt ttgtgatgta gttcttatct gtaactcccg gaatgatacc aaaacgtttc
     1141 tgtaagcatt ttgcaaactt gtaagttgtt gattccattg gtgttccgta tacagaatag
     1201 ctgatgttct ctgctgcctt ccattcatta catttatcat ttaatttctg cataactttt
     1261 aatgcaaatt cttttccttc atcggatgta tgagacttgc ctgtcatgcg gacacacatc
     1321 tcataaagtc ctgcatatcc aagggaaatc gtggaatatc catcgtataa taacttatcg
     1381 atcttctcgc ctttcttcag tctcgcaagc gctccatgtt gccaaaggat cggcgcaaca
     1441 tcagataccg ctccctttaa tctctcatgg cgacatctta atgctctgtg gcacaattcc
     1501 agtctctctt ctaagatgtc ccagaattta tccatatctc cattagaact acatgcgaca
     1561 tcaactaagt tgatcgttac aacaccctga ttaaatcttc catagaactt atgacttcca
     1621 tctgcatttc tctgggaatc ttcaaccgtt aagaaggatc tgcatcccat gcaaggatat
     1681 acttcaccat ttttcagttc tctcatcact ttcgctgaga tatagtctgg aaccatgcgt
     1741 tttgctgtac attttgcagc taattctgtc agatgccaat acttggaatc tggtgtgatg
     1801 ttatcatcat ctaatgcata aataagtttt gggaaggctg gagtgatcca gactcctttt
     1861 tcattcttaa caccctgaat tctctgtttc aggacttctt cgatcacaag agccagatcg
     1921 tctcttgtct gtccttctgg tacttcatca agatacataa agactgtgac aaatggcgcc
     1981 tgtccattac atgtcattaa agttaccagc tgatactgga tcgtctggat tccctgtttg
     2041 atctcatcat acagacgcat ctctgtaatc ttgtcgatga tcgcatcatc catagattct
     2101 ccgatcactt tacgttcctt cacgacattc ttacgcagct tctttcgact gatatctaca
     2161 aatggtgcta aatgtgctaa tgtaaaagac tgtcctccat actggttgct tgcaacctga
     2221 gcaacgatct gtgttgtcac attacatgct gtaaaaaatg aatgtggttt ctcgatcaat
     2281 gtctcactga tcaccgttcc attctgaagc atatcttcca agttgatcag atcacagtta
     2341 tgttctctct gtgcaaaata gtcagaatca tgaaaatgga tgattccttc ttcatgagca
     2401 cgaatgatct catttggcag aaggacacgt ttacttaagt ctttgctgac ttctcctgcc
     2461 atataatctc tctgcgtgga attgatcact gggttcttat tcgaattctc ctgatttact
     2521 tcctcattaa tattatcaag taaagaaaga attccatcat cggtcgtatt tgattttctt
     2581 ctcagttctc tcttaaaacg atatcttaca tacttctggg caacttcata tcctctcatc
     2641 tccatgatcc ctgtctcgac catattctgg atgtcttcta cattcaccgc atgagaagag
     2701 tttgctacct gatctgcaac cttctctgcg atcgccatga tctgatactg attcatctga
     2761 tggatctttg ttacctcttc attcgccttc gtgatcgcat tgatgatctt atcgagatca
     2821 aaggttacct cctcaccgtt tctcttgata atctttgttt ttacatcacc attcatagtt
     2881 tctccctcat ttatataaaa ttacaagatt ctgtatgttt ccattttctg acacaaaatc
     2941 ttgttgtcgt tctaccaatc tgcactatat cttgtgccat tgcctttatt atattacact
     3001 ctttttcata aaaaagaaag agctaatttt tcattttaga gaatatgaag atgctcaaac
     3061 agaatcttaa atcccaaaag tatcagtatt atgccacctg cgagctctgc cttggattta
     3121 tattttgttc caaaaatatt tccgatcttt actcctacaa atgaaatcaa gaaagtacag
     3181 actccaatca tcgacgctgc ggatacaata tgcacattta aaaaggcaaa tgtgatccca
     3241 actgctaatg catcgatact tgtcgcaaca gcaagcatga acatttcttt cactgcgatc
     3301 gaatcatctg cctgttcttc atcatttgat aatgcttctt tgaccatatt gattccgatc
     3361 agcccaagaa gcacaaatgc gatccaatgg tcgatcgatg tgatctggtc tttaaactgt
     3421 gttccaagga aaaatccgat tgttggcatc agtgcctgaa atcctccaaa ccataaacca
     3481 acaatgatcc cttttttcaa aactttttct ctcatcgcca gtcctttgca gatagaaaca
     3541 gcaaatgcat ccatcgccaa tccaacagcc agtgttaata acgtaagtaa atccataaaa
     3601 acgtcctcca tatcgtaagt ttttcatatc ataggggatt ccatcggaat ttcctatgat
     3661 atgaaaaaga cccacagacg aaagccttgt tataaacaaa agcttatcgt ctgtgagtct
     3721 cgttaattaa gacagcacca gatgtcgttc atcattatgt tgatactgtc acagattctc
     3781 tgctaactac tccctcacgg aaatatctat aagatcatac cggatgtgct tccggcaaaa
     3841 tgtgcctatg tatcatatct caactgagaa tctttgtcaa tatctttcct ttaatttttg
     3901 attacgcttt tcaagatcca acaggtattc tttcatttcc agaccacctg catatcctgt
     3961 cagtgaaccg ttcattccga tcactctgtg acatggaaca acgatcacga tcggattcct
     4021 gttgttcgcc ataccgacag ctcgacatcc tttgggaatt cctactgatt ccgcaatgtc
     4081 tttgtaagtg taagtttccc catatggaat cttctccaat gctttccata ctttgatctg
     4141 aaattctgtt ccatgataag aaagtttcag gtcaaacttc tttctccgtc catcaaaata
     4201 ctgtgataat tgcagtgctg cttccctgat caattctgta ttcttcttct cgccttctaa
     4261 tgtcttcgtc ccaaaatata tcccgcaaat gccatgccca tcttcctcaa tcgttaaatt
     4321 accgattgga aaactataat cataatagta cctcatatga caatcctcct aaaaataggt
     4381 ataccatatt cttccatggt tggcaaggac taacttgaca tgtacatatt tcttgcgtac
     4441 aatgaaaaga actgtataaa aatgaacaga tatttatttt aggaggaatt ttcattggac
     4501 cccagtatga tattacagat aatcgcaatt atcattttat taatgctttc tgcttatttt
     4561 tcatctgcag aaacagcact tacaaccgtc aatccacata gtatgcgtgc catggcagaa
     4621 gatggcaata aaaaagctgc tattgtttta aaattgatcg agaatccaag ccagatgctt
     4681 tccagcgtct tagtcggaaa taaccttgtg aatatttcat taacatccct tactacaacg
     4741 atcgctattc gtatctttgg aagcatggga gccggaatcg caactgctgt gatcacagta
     4801 cttgtattga tctttggtga gatcacacca aagacttttg caacgatcaa taatacaaaa
     4861 cttgctctaa gctatgcttc cagcatttat ctgatcacaa aattactgac accggttgtt
     4921 tacattatca acaaactttc tacagcgatc cttatcatct tgcggatcga ctcgaaacaa
     4981 tccaaacgta tcatgactga acgtgaactt cgtacgatcg ttgatgtcag ccacgaagaa
     5041 ggcgttatcg aagaatctga gaaagatatg ttaaacaacg tttttgattt taaggaatct
     5101 cttgccaagg atattatgat cccgcgaatt gatgtaactt ttgtgtctgt agatgcttct
     5161 tatgatgaag ttcttgatat tttccgcgag gccggttatt ctagacttcc tgtttatgaa
     5221 ggaagtaaag atcatgttgt tggtattgtt tattttaagg atgtttattt ctaccgcagc
     5281 cagcacagag gcgagatctt tcatttgaaa gatgttctgc gtaagccgtt ctttacgtat
     5341 gaaactcaga aagtatcaag ccttcttgca cagatgcgtg aaaaatctgt tagtttctcc
     5401 attgttttgg atgaatatgg agtgactgca ggtcttatta ctctagaaga tatcgttgaa
     5461 gagatttttg gagaactgcg tgatgaatat gatgattctg aggaagaaac tttcaaaaaa
     5521 ttatccgatg gatcttatat ggtatccgct tctttaaagc ttgatgatct tgatgatctg
     5581 cttgacgtag accttgattc tgaggattat gattctcttg gcggatatat catcgaactg
     5641 cttgatcatc tgc
//
LOCUS       DAOMUE010000116         5644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3921_length_5644_cov_2.318841, whole genome shotgun sequence.
ACCESSION   DAOMUE010000116 DAOMUE010000000
VERSION     DAOMUE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3921_length_5644_cov_2.318841"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLC71_06325"
     CDS             <1..364
                     /locus_tag="PLC71_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HRM02291.1"
                     /translation="NKYGFDKDAHFKYIDKILNRFKNPYLVDDTARVGREPLRKLSAT
                     DRLTKPTMTALEYGLPVDALLAGMAAALKYDNAEDPQSVELQDKIKANGVKAALKEVS
                     GIADEKILDEVVAIYEAM"
     gene            complement(496..2238)
                     /locus_tag="PLC71_06330"
     CDS             complement(496..2238)
                     /locus_tag="PLC71_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HRM02292.1"
                     /translation="MAFDGFVISNIISELNKQIIGGRIYKIYQPEADEITLVIKKERL
                     TTRLHLSASASLPLVYLSKEGKANPMQAPNFCMLLRKHISSGRIISITQPDFERIIEI
                     KIEHLDELGDVCQKRLIVEIMGKHSNIIFVNSDDVILDSIKHISANISSVREVLPGRT
                     YTFPPSKGKHPLASLTAENFYQHIITKPLNLCKALYTSITGMSPLIANELCYRAGLDG
                     NASTDSLTDDSAAGLYGQLVKLNALVEDEAYQPNIIYSGEEPKEFSCIPLTSYPDCTE
                     KTYDSIFDVLIGYYSEKEKYTRMKQKSSDLRKIVQTALERTSKKYDLQLRQLKDTEKR
                     EKFKVYGELTQTYGYGLEANAKSLTCMNYYTNEEITIPLDPTKTPLENSKKFFAKYNK
                     LKRTYEALSELVVETKADLDHLDSISTALSLSEDEKDLQLIKDELSDYGYIKSHGKKK
                     GKKQAKSKPLHFVSSDGFHMYVGKNNYQNDELSFKFANGGDWWFHAKNMAGSHVIVRK
                     EQAETLPDATFEEAGRLAAYYSKGRQAPKVEIDYVQRKELRKPPKAKPGFVIYHTNYS
                     MMCVPNIDGIEIVE"
     gene            2454..3467
                     /locus_tag="PLC71_06335"
     CDS             2454..3467
                     /locus_tag="PLC71_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02293.1"
                     /translation="MRKEASKNAWKQLYDITLKLDKLEPWNYLGDTQLVTIQLKDREE
                     PVFCSVMGKFSGERGIAVFDGVEGLGDFYMILGSEEGDLPAQYLMDEHSSLTCFWGSM
                     MNVPDEQRKVIDELDIRFKTISDWIYFVSYKKGYKPYQLDEDEVALLIETYENLYMAV
                     EAVRQGELNPEIEQAEGIVRRYNTENDTWEMFVKEIPEAEKEFPSVMLEDQELKDELK
                     DQKQIDLEVILDFTYLPVMVEGEEEGERPKNPLLFMAYDNTDGGVITMDILEEGDDEI
                     SRTLGFFISFVQQNGRMKTIKARNPWIFGALEDICEYCSVDLVYDPVEIMDQVIEEVV
                     SQL"
     gene            3608..4789
                     /gene="hisZ"
                     /locus_tag="PLC71_06340"
     CDS             3608..4789
                     /gene="hisZ"
                     /locus_tag="PLC71_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRM02294.1"
                     /translation="MNKKRVHTPEGVRDVYGTEYGQRSKLKAKLNKVFASYGYEGIRT
                     PGFEFFDVFNSEKGTVSAREMFKFFDREGNTLVLRPDVTPSIARCIAKYFNEENMGIR
                     LSYAGNVFLNNSSYQLKLKETCQLGAELVNDASVQADTESIAMAIDCFLAAGLTDFRL
                     SIGEVEFFNGLTQTIKDEEVKTQLRELILNKNYFGLMEYVETLDLSDKIKDVFLHFNE
                     LNGDVAILDQAEAMVENEQSKKAIKRLRDVYDILKLYGKEQYVSFDLGMLNKHNYYTG
                     IIFKGYTYGTGDAVLKGGRYDNLIEQFGKKAPSVGFAIVLDELMMALSRQGIHMEADH
                     MDTMIIYKEATMKDAILRAEELRKEGKKVILERKNDLCSKADYERFAKEHRLGGILYF
                     I"
     gene            4826..5473
                     /gene="hisG"
                     /locus_tag="PLC71_06345"
     CDS             4826..5473
                     /gene="hisG"
                     /locus_tag="PLC71_06345"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRM02295.1"
                     /translation="MRYITFALAKGRLAKKAMALLEEIGITCEEMKDEKTRKLIFVNE
                     ELKLKFFLSKASDVPTYVEYGAADIGVVGKDTILEEGRNLYEVLDLKFGACKMCVCGP
                     KEAKKYLNSNEIIRVASKYPHIAKEYFNNQKLQTVEIVKLNGSVELAPIVGLSEVIVD
                     IVETGTTLKENGLEVLEEVCPLSARMVVNQVSQKMEQERISKIIVALKKILDEKE"
BASE COUNT         1984 a          901 c         1166 g         1593 t
ORIGIN      
        1 caacaaatat ggatttgaca aagatgctca ctttaaatac atcgacaaga tcttaaatcg
       61 tttcaaaaac ccataccttg tagatgatac agctcgtgta ggtagagaac cattaagaaa
      121 attaagtgca acagatcgtt taacaaaacc aacaatgaca gcattagaat atggtcttcc
      181 agtggatgca ttattagcag gaatggcagc agcattaaaa tatgataatg ctgaagatcc
      241 acagagcgta gaattacagg ataagatcaa agcaaacgga gtaaaagcag cattaaaaga
      301 agtatctgga atcgcagatg aaaaaatctt agacgaagtt gtagcaattt acgaggcaat
      361 gtagtcggaa caatatcaat aaaaaatcaa ccaaaaatca aattaacaac cttttttctt
      421 aactcatata atcatatgaa aagaggcggc cgaaaatatt tcggtcgcct cttttggcat
      481 tataaataca actgtctatt caacaatttc aattccatca atattcggaa cacacatcat
      541 agaatagttt gtatgataaa tgacaaatcc aggttttgcc tttggcggct ttcttagttc
      601 ttttctctga acataatcaa tctcaacctt cggtgcctga cgccccttgg aataataagc
      661 tgcaaggcgg ccagcttctt caaaggtagc atctggaaga gtctccgctt gttcctttcg
      721 tacaataaca tgggacccag ccatattctt tgcatggaac caccagtccc cgccatttgc
      781 aaacttaaaa cttaattcat cattctgata gttatttttt ccaacataca tatggaaacc
      841 atcagaagag acaaaatgta atggtttgga ttttgcctgt ttctttcctt ttttctttcc
      901 gtggctttta atataaccat aatcagaaag ttcatctttg atcagctgca ggtctttctc
      961 atcttcggat aaagaaagag cagtagagat cgaatcgaga tgatcaagat cagcttttgt
     1021 ctccacgaca agctcagaaa gagcttcata agtacgtttt agcttattat attttgcaaa
     1081 aaactttttg gaattctcca aaggagtctt tgttggatca agagggatcg taatctcttc
     1141 gtttgtgtaa taattcatgc aagttaaaga ttttgcattt gcctctagcc catatccata
     1201 tgtctgtgta agttctccat aaactttgaa tttctcacgt ttctcggtat cctttaactg
     1261 acgaagctgc agatcatatt tcttgcttgt cctctcaaga gcagtctgca cgatcttacg
     1321 aaggtcagaa gatttctgct tcattcttgt atatttttcc ttctcagaat aatacccgat
     1381 cagcacgtca aaaatagaat catatgtttt ctctgtacag tcaggataag aagttaaagg
     1441 aatacaagaa aattccttag gttcttcccc agaataaatg atatttggct gatatgcctc
     1501 atcttctaca agagcattta attttacaag ctggccatat aatccagcag cactatcatc
     1561 ggtcaaagaa tcagtagatg cattcccatc gagccctgca cgataacaaa gttcatttgc
     1621 gatcaaagga ctcatacctg tgatggaagt ataaagtgct ttacaaagat tcagtggttt
     1681 ggtgatgata tgctgataaa aattctccgc agtcagactt gctaaaggat gtttcccttt
     1741 agacggtgga aatgtgtaag tacgtccagg taaaacctct ctgacagaac tgatatttgc
     1801 agagatgtgc ttgatactat ctaagatcac atcatcactg tttacaaaaa tgatattact
     1861 gtgttttccc atgatttcaa cgatcagacg tttctgacag acatctccaa gctcatccag
     1921 atgctcaatc ttaatttcga taatacgctc aaagtcaggc tgtgtgatcg aaatgatcct
     1981 tccactgctg atatgtttac gaagcagcat gcagaagttt ggagcctgca tagggtttgc
     2041 ttttccttct ttcgataaat agacaagagg aagagaagca ctggctgaga gatgaagtct
     2101 tgttgtcaat ctctcttttt tgatcacaag cgtgatctcg tcggcctctg gctgataaat
     2161 cttatagatt ctgccaccga tgatctgttt attaagttct gatataatat ttgaaatgac
     2221 aaatccgtcg aatgccatga taaatacctc ctggtaataa aatcagttat ctaatttaaa
     2281 ataataagat aaatccgtca aacaattata tcagaaattc gaaaaagtat aaagaaaaaa
     2341 cgatcaaatt cacaaatcat agaaagatag tcgggatttt gttgtaatta aaatcatttc
     2401 ttattataat gaatattaag tatacaataa aataatctaa ggaggacaag tcattgagaa
     2461 aagaagcgtc aaagaatgca tggaagcagt tatacgacat tacattaaaa ctagacaaat
     2521 tagagccttg gaattacctt ggtgacacac aacttgtaac cattcagcta aaagacagag
     2581 aagaacctgt attttgcagt gtgatgggaa aattcagcgg agaaagagga attgcagtct
     2641 ttgatggagt tgaaggctta ggagatttct atatgatcct tggaagtgaa gaaggggatc
     2701 ttccagcaca gtatctgatg gatgaacatt caagtcttac atgcttctgg ggaagcatga
     2761 tgaatgtgcc agatgaacaa agaaaagtta tcgatgaatt agatattcgt tttaagacga
     2821 tcagtgactg gatttacttt gtttcttata agaaaggtta caaaccatat cagttagatg
     2881 aagatgaagt tgcacttctt atagaaactt atgagaacct gtatatggca gtggaagcag
     2941 tcagacaggg agaattaaat ccagagatcg aacaggcaga aggaatcgtt cgccgttata
     3001 atactgaaaa tgatacttgg gaaatgtttg taaaagagat accagaagct gaaaaagaat
     3061 ttccgtcagt gatgttagaa gatcaggaat taaaagatga attaaaggat caaaaacaga
     3121 tcgatcttga agtgatttta gattttactt atcttccagt tatggtagaa ggggaagaag
     3181 aaggggagcg tccaaagaat ccacttttat ttatggcata tgataatacc gatggcggtg
     3241 tgatcacaat ggatatttta gaagagggtg atgatgagat cagcagaaca ttaggattct
     3301 ttatcagttt tgtgcagcag aatggaagaa tgaagacgat caaagcaaga aatccatgga
     3361 tttttggagc attagaggat atttgtgaat attgcagcgt ggaccttgta tatgatccgg
     3421 tagagatcat ggatcaggtg atcgaagaag ttgtttctca gttgtaggaa tttcatgtaa
     3481 catatcaaaa gatgaattag agtgaacgag gttgacaaat aagttaagga ttggtaaaat
     3541 acagataatt tgatgtgcta cctaagtagc actttaattc gataaagtat caaggagcgg
     3601 caaaaatatg aataaaaaga gagtccacac accagaaggt gtcagagatg tatatggaac
     3661 agaatatgga cagcgaagta agctgaaggc aaaattaaac aaagtttttg catcttacgg
     3721 atatgaaggg atcagaactc caggatttga attctttgat gtatttaaca gtgaaaaagg
     3781 aaccgtatcc gcaagggaaa tgtttaaatt cttcgataga gaaggaaata ccttagttct
     3841 tcgtccggat gtcacaccat ccattgcacg ctgtatcgca aagtatttta atgaagagaa
     3901 tatgggaatc cgtttatctt atgcaggaaa tgtattttta aataattcaa gctatcagtt
     3961 gaagttaaaa gaaacatgcc agttaggtgc agaattagtc aatgatgcgt ctgtacaggc
     4021 agatacagaa tctattgcaa tggcaattga ttgttttctg gcagctggat tgactgattt
     4081 cagactttct atcggagaag ttgaattctt caacggattg acacagacga tcaaagatga
     4141 agaagtcaaa acacagcttc gagagctgat cttaaataag aattattttg gattaatgga
     4201 atatgtagag acattagatc tgtctgataa gatcaaagat gtatttttac attttaacga
     4261 actgaatgga gatgtagcga tcttagatca ggcagaagca atggttgaga atgaacagtc
     4321 taagaaagcg atcaaaagac ttcgcgatgt atatgatatc ttaaaattat acggcaaaga
     4381 gcagtatgta agttttgatc ttggaatgtt aaataagcat aattattaca caggaatcat
     4441 tttcaaaggt tatacttatg gaacaggaga tgcagttcta aaaggtggac gatatgataa
     4501 tctgatcgaa cagttcggaa agaaagcccc atcagttgga tttgcgatcg tattagatga
     4561 gctgatgatg gcattaagcc gtcagggaat tcatatggaa gcagatcata tggatacaat
     4621 gatcatttat aaagaagcaa caatgaaaga tgcgatcctt agagcagaag aattaagaaa
     4681 agaaggaaag aaagtcatct tagagagaaa gaatgatctg tgcagcaaag cagactatga
     4741 aagatttgct aaagagcatc gcctcggtgg aattctttat ttcatctaat caacaaatcg
     4801 agcaaaagaa gggaaacaaa agaccatgag atatattaca tttgccctgg cgaaagggcg
     4861 ccttgcaaag aaagcgatgg ctttattaga agagatcgga atcacatgcg aggaaatgaa
     4921 agacgagaaa acaagaaaat taatcttcgt aaacgaagaa ttaaaattaa aatttttctt
     4981 atccaaagca agtgacgtgc caacttacgt ggaatatgga gcagctgata tcggtgttgt
     5041 tggaaaagat acgatccttg aagaaggaag aaatctatac gaagtattag acttgaaatt
     5101 tggagcttgc aagatgtgtg tgtgcggacc caaagaagcg aagaaatatt taaacagtaa
     5161 tgagatcatc cgtgtggcat ccaaataccc acatatcgcc aaagaatatt tcaataatca
     5221 gaaattacag acagtagaga tcgttaagtt aaacggatct gttgaacttg caccgatcgt
     5281 tggattatca gaggtgatcg tcgatatcgt tgagacagga acaaccttaa aagaaaatgg
     5341 attggaagtc ttagaagaag tttgcccatt atcagcaaga atggtagtga atcaggtaag
     5401 ccagaagatg gaacaagaac gaatcagcaa gatcatcgtt gcactaaaga agatcttgga
     5461 tgaaaaagaa tagtccggag gagagaaata tgagaatagt aaaagttaac aaagattcca
     5521 ttgcaaatat tttatcagat ttattaaaga gaagtcctac aaactatggg gattttcagg
     5581 ataaagtcga tgcgatcatc aaaaatgtca gagataatgg agataaagct gtctttgatt
     5641 atac
//
LOCUS       DAOMUE010000117         5636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3933_length_5636_cov_1.929045, whole genome shotgun sequence.
ACCESSION   DAOMUE010000117 DAOMUE010000000
VERSION     DAOMUE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3933_length_5636_cov_1.929045"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1387
                     /locus_tag="PLC71_06350"
     CDS             146..1387
                     /locus_tag="PLC71_06350"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRM02296.1"
                     /translation="MLEQLGKQAKEASIILGKAGIEDKNEVLREAAKCLVQNQEYILS
                     ENYKDVENAKSNDMSEALIDRLSLNPDRIEAMAEGLRQVAELHDPIGSVEHMQKTPNG
                     LLIGKKTVPLGVVGIIYESRPNVTADAFALCFKTGNACLLRGGSDAINSNLAIINVIQ
                     SALESCNMPKYSIQLLTDTSRETATKMMQLNEYLDVLIPRGGAGLIQSVVKNSTVPVI
                     ETGTGNCHVYVDEFAQKDMAVNIIINAKTQRLGTCNTCESLVLHKNILESHGPAIIDA
                     LLEKGVQVRGDERVCALNDQVIPATEEDWGTEYLDSIISVKTVDSFDEAVAHINHYNT
                     GHSEAIVTDSYQNAQEFLNQIDAAAVYVNASTRFTDGFEFGFGAEIGISTQKIHARGP
                     MGLDALTTSKFIIYGNGQIRK"
     gene            1609..>2507
                     /locus_tag="PLC71_06355"
     CDS             1609..>2507
                     /locus_tag="PLC71_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HRM02297.1"
                     /translation="MALFTFSGGVHPADGKEFAKNSPITEYLPKGDVVLPLGQHIGAP
                     AQPIVSKGDQVLVGQKVAEAGGFVSANIFSSVSGTVKAIEPRMTPAGAKVNSIVIEND
                     GEFKEAAFEAKPYDQMSKDDVLAAIKEAGIVGLGGAGFPTHVKLAPKDPDAIEYIIVN
                     GAECEPYITGDYRILMETPELLIEGLNIVLDMFPKAKGIIGIEDNKKDAIAKVEALVK
                     GDARISVATLKTKYPQGAERSLIYATTGRSINSSMLPADAGCIVDNVATIVAIKEAVK
                     DGKPLYERVVTVTGDAIKNPSNF"
     assembly_gap    2508..2517
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2518..2930
                     /locus_tag="PLC71_06360"
     CDS             <2518..2930
                     /locus_tag="PLC71_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRM02298.1"
                     /translation="TGTNVQELIEAAGGFKTQPEKIISGGPMMGMAMFTTDVPAVKTF
                     SCFLAFTKDEVAANQPSNCIRCGRCVSVCPQKLMPAKLSVLADHNQFDEFEKLYGAEC
                     VECGSCSFVCPAKRNLAQSMKTGRKQVLANRRKK"
     gene            2944..3891
                     /locus_tag="PLC71_06365"
     CDS             2944..3891
                     /locus_tag="PLC71_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HRM02299.1"
                     /translation="MSETLYHVSANPHVRDNGSTQKIMLDVIIALLPATIFGIYIFGA
                     SAALTVAISVATCVVVEYIYQKLMKQPITVGDLSAALTGLLLALNLPSGAPFWMPILG
                     GIFAIIVVKQVFGGLGQNFMNPALGARCFLMISFAARMTTFTYDGVTTATPLAVMEQG
                     KSVDVMKSFLGFVPGTICETSALLLIIGGAYLVFKKVISPIIPCVYVAVVAIFVLIFG
                     GHGFDLSYMAAQIFSGGLMLGAIFMATDYVTSPITKNGKIIYAIVLGILTGLFRLFGP
                     MAEGVSYSIIIANLLVPLIERVTVPTPFGIVKKGGKSNE"
     gene            3884..4495
                     /locus_tag="PLC71_06370"
     CDS             3884..4495
                     /locus_tag="PLC71_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HRM02300.1"
                     /translation="MNKNLVMDCIKLCVITLIAGLLLGIVYNVTKAPIAAQEEKTKQE
                     AYKAVFKDAAKFDTVKLDQKDVAAVLKKNDLGQNTISEVVKAVDKNGKELGYVFSVTN
                     PEGYGGDVSLSVGVRDDGTVNGYETLSISETAGLGMKAKEPEFKSNFKNKKADKFEVV
                     KDGSGKNDDSKVDAISGATITSRAVTSAVNSCVAYSKSLKGGN"
     gene            4498..5202
                     /locus_tag="PLC71_06375"
     CDS             4498..5202
                     /locus_tag="PLC71_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HRM02301.1"
                     /translation="MKPNPMERLINGIVKENPTFVLMLGMCPTLAVTTSAINGAGMGL
                     STTAVLMFSNMIISALRNVIPDRVRIPGYIVVIASLVTIVQFLLQGYVPAVNDALGLY
                     IPLIVVNCIILGRAESFAAKNGPINSFFDGLGMGLGFTVALTILGAFRELLGAGQIFG
                     MQVMPSAYEPITIFILAPGAFFVLACLVAIQNKVKSKKPKKAGEATSGGCGDCSHCGN
                     SACGSRSFFDTTVDDK"
     gene            5214..>5636
                     /locus_tag="PLC71_06380"
     CDS             5214..>5636
                     /locus_tag="PLC71_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HRM02302.1"
                     /translation="MKSLLLIAIGSALVNNVVLSQFLGLCPFLGVSKKVETAAGMGAA
                     VLFVITIASAVTNLIYTFLLVPTDLTYLNTIVFILVIAALVQFVEMVLKKSMPALYES
                     LGVFLPLITTNCAVLGTALTNVQNDYSFIASVVNGIGIS"
BASE COUNT         1893 a         1002 c         1234 g         1497 t
ORIGIN      
        1 gtgcaaactt aagagcacag cttaataatg taagtatcaa aaatgaagat ggatcgatca
       61 ctccattaaa accaaaagga ttaaactaga gcagataaaa cagatggaga tagatgcaaa
      121 ccgcaccgaa aaggaggcat tcataatgtt agaacaacta ggaaaacagg caaaagaagc
      181 atcgataatt cttggaaaag cagggattga agataagaat gaagtattaa gagaagcagc
      241 caaatgtctg gtacagaatc aggaatatat cttaagtgag aattataaag atgtagagaa
      301 tgcaaaaagc aatgatatga gcgaggcgtt gatcgatcgc ttatccctga atcctgaccg
      361 tattgaagca atggcagaag gacttcgtca ggtagctgaa ctacatgatc caattggttc
      421 tgtagaacat atgcagaaaa caccgaatgg acttttgatc ggtaagaaaa cagtaccatt
      481 aggagttgtc ggtatcatct acgaatcaag accaaacgta acagcagatg catttgcact
      541 atgttttaag acaggaaatg catgccttct tcgtggtgga agtgatgcaa tcaattcaaa
      601 tcttgcgatc ataaatgtga tccagagtgc actagaatcc tgcaatatgc caaaatacag
      661 cattcagctg ctgacagata caagccgtga gacagctacg aagatgatgc agttaaatga
      721 atacttagat gttctgatcc caagaggtgg tgcaggactc attcagagtg ttgtaaagaa
      781 ttctacagtg ccagtgatcg agacaggaac aggaaattgt catgtatacg tagatgaatt
      841 tgcccagaaa gacatggcag ttaacatcat catcaatgca aagacacaga gacttggaac
      901 atgtaataca tgtgaatctt tagtattaca taagaacatc ttggagagtc acggtccagc
      961 gatcatcgat gctttattag aaaaaggagt acaggtacgt ggagatgaga gagtttgtgc
     1021 tttaaatgac caggtcatcc ctgcaacaga agaagactgg ggaacagaat acttagattc
     1081 tatcatttct gtgaaaactg tcgatagctt tgacgaagca gtggcacata tcaatcatta
     1141 caatacgggg cattcggaag cgatcgtcac agattcttac cagaatgcac aggaattctt
     1201 aaatcagatc gatgcagcag ccgtttatgt taatgcatcc acaagattta ctgatggatt
     1261 tgaatttgga tttggagcag agatcggaat cagtacccag aagattcatg ccagaggacc
     1321 aatgggatta gatgcactga caaccagcaa atttattatt tatggaaatg gacagatccg
     1381 taaatgatag aatataaaag ccagaattgt aaattatttc agaatcttaa gaaaatctga
     1441 agaaaaacat agaattttgt atgcaaatgc aaagaaattg ttaaaaaaac attgaaaaat
     1501 aaaaaagact atggtattat aagtcttgtg ttcaaaggag ttttcatttt atgagaactc
     1561 ttttttttcg caatggaaaa aaatgtaaag aagaaaggaa ggcttaaaat ggcattgttt
     1621 acatttagcg gcggtgttca tccggcagat ggtaaagaat ttgcaaagaa ttcaccaatc
     1681 accgaatacc tgccaaaagg agatgttgtt ttacctttag gtcagcacat cggtgctcct
     1741 gcacagccaa tcgttagcaa gggagaccag gtgttagtag gccagaaggt ggcagaagct
     1801 ggaggatttg tatccgcgaa tattttcagc tctgtttcag gaactgtaaa ggcgatcgag
     1861 ccaagaatga ctccagccgg agcaaaagtt aactctatcg taattgagaa tgatggagaa
     1921 ttcaaagagg ctgcctttga ggcaaaacct tatgatcaga tgtctaaaga tgatgtttta
     1981 gcagcgatca aagaagctgg aatcgtagga cttggtggag ccggattccc aacacatgtg
     2041 aaattagcgc caaaagatcc agatgcgatc gaatatatca tcgtaaatgg agcggagtgt
     2101 gaaccttata tcacaggtga ctaccgcatc ttaatggaga caccagaact tttaattgaa
     2161 ggattaaata tcgtacttga tatgttccca aaagcaaaag gtatcattgg tattgaagat
     2221 aataaaaaag atgcaattgc taaagtagaa gcattagtaa aaggtgatgc aagaatcagc
     2281 gttgctacat taaagacaaa atacccacag ggtgcagaac gttctttgat ttatgcaaca
     2341 acaggaagaa gcatcaactc cagcatgtta ccagcagatg caggatgcat cgtagataac
     2401 gtagctacga tcgtagcgat caaagaagct gtcaaagatg gtaaaccatt atacgaacgt
     2461 gttgtgacag ttactggaga tgcgatcaag aatccaagca actttaannn nnnnnnngta
     2521 caggaacaaa cgtacaggaa ctgatcgaag cagccggtgg atttaaaaca cagcctgaga
     2581 agatcatttc tggtggacct atgatgggta tggcaatgtt tacaacagat gtaccagctg
     2641 tcaaaacatt ctcatgcttc ttagcattca caaaagatga agttgctgcg aaccaaccaa
     2701 gcaattgtat tcgttgtgga agatgtgtaa gcgtctgtcc acagaagctg atgccagcaa
     2761 aattatctgt attagcagat cataatcagt ttgacgagtt tgagaaactt tatggagcag
     2821 aatgcgttga gtgtggatct tgttcatttg tatgtccagc aaaacgtaac cttgcacagt
     2881 ccatgaagac aggtcgtaaa caggtattag ctaaccgtag aaagaaataa aggagagata
     2941 catatgagcg aaacattata tcatgtgtca gccaatcctc atgtcagaga taatggctca
     3001 acacaaaaga ttatgttaga tgttatcatc gcattactac ccgctacaat ttttggtatc
     3061 tatatctttg gagcatctgc ggcactgaca gttgcaattt ctgttgctac ctgtgttgtc
     3121 gttgagtata tttaccagaa acttatgaaa cagccaatca ctgtaggtga cttaagtgcg
     3181 gcattgaccg gactgttact tgcattaaac ttaccatcag gagcaccatt ctggatgccg
     3241 atccttggtg gaatcttcgc gatcatcgtt gtaaaacagg tgttcggtgg attgggacag
     3301 aactttatga acccagccct tggtgcaaga tgtttcttaa tgatctcatt tgcagcaaga
     3361 atgacaacat ttacatatga tggtgtgaca acagcaacac cacttgctgt tatggaacag
     3421 ggaaaaagcg tggatgttat gaaatcattc ttaggattcg ttcctggaac aatttgtgaa
     3481 acatctgcat tattactgat catcggtgga gcatacttag tatttaagaa agtcatctct
     3541 ccgatcatcc catgtgttta cgttgcagtt gtagcaatct tcgtactgat cttcggtggt
     3601 catggatttg atctatctta catggcagca cagatcttca gtggtggatt aatgttaggt
     3661 gcgatcttca tggcaacaga ctacgtaaca tctccaatta caaagaacgg aaagatcatc
     3721 tatgcgatcg ttcttggtat tttaactgga ttattccgtc tgttcggacc aatggcagaa
     3781 ggtgtatctt actcaatcat cattgcgaac ttattagttc cactgatcga gagagtgaca
     3841 gtaccaactc catttggaat cgtaaaaaaa ggaggtaaga gcaatgaata agaatctcgt
     3901 aatggattgt atcaaattat gtgtgatcac attaattgca ggactccttt taggtatcgt
     3961 atataatgta acaaaagcac cgatcgccgc acaggaagaa aagacaaaac aggaagctta
     4021 caaagccgta ttcaaagatg cagcgaaatt cgatacagta aaattagacc agaaagacgt
     4081 agcagctgtc ttaaagaaaa atgatcttgg acagaacacg atttctgaag ttgtaaaagc
     4141 agttgacaaa aacggaaaag aacttggata cgtattctct gtaacaaacc cagaaggata
     4201 cggtggagat gtatctttat ctgtaggtgt tagagatgat ggaacagtca atggttatga
     4261 aacattatcc atcagcgaaa cagctggttt aggtatgaaa gccaaagaac cagaattcaa
     4321 gagtaacttt aaaaacaaaa aagcagacaa atttgaagtt gtaaaagatg gatctggtaa
     4381 gaacgatgat tctaaagtag atgccatcag tggtgcaacg atcacaagcc gtgctgtcac
     4441 aagtgcagtt aacagctgtg ttgcatattc aaagagcctg aagggaggta attaaacatg
     4501 aaaccaaatc caatggaacg attgattaat ggtatcgtaa aagaaaaccc taccttcgtc
     4561 ttaatgttag gtatgtgtcc tacattagca gtaacaacat ctgcgatcaa cggtgcagga
     4621 atgggacttt caacaacagc cgtattaatg ttttctaaca tgatcatttc agcattaaga
     4681 aatgtcatcc ctgatcgtgt acgtattcca ggatatatcg tagttatcgc atctctggta
     4741 acaatcgtac agttcctatt acagggatat gttccagcag ttaacgacgc attaggatta
     4801 tacatcccac tgatcgtagt aaactgtatc atccttggac gtgcagaatc ctttgcagca
     4861 aagaacggac cgattaactc cttctttgac ggacttggaa tgggacttgg atttactgta
     4921 gccttaacaa ttttaggagc attccgtgaa ttattaggag caggtcagat cttcggaatg
     4981 caggtaatgc catctgcata cgaaccaatc acaatcttta tcctggcacc tggagcattc
     5041 ttcgtacttg catgcctagt agcgatccag aataaagtta agagcaaaaa acctaaaaaa
     5101 gccggagaag caacaagtgg tggatgcgga gattgttctc actgcggaaa cagcgcttgc
     5161 ggttccagaa gcttttttga tacaactgta gatgacaaat aaggaggatt aagatgaaaa
     5221 gtttactgtt aattgccatc ggttcagcgc tggtaaataa cgtcgtatta agccagttcc
     5281 ttggtttatg tcctttcctt ggagtttcta agaaagttga gacagcagcc ggaatgggtg
     5341 cagccgttct cttcgttatt actatcgcat cagcagttac aaatttaatt tatacatttt
     5401 tattagtacc aacagattta acatacttaa atacgatcgt atttatcctg gtaatcgcag
     5461 cattagtaca gttcgtagaa atggtattaa agaaatctat gccagcatta tatgaatctc
     5521 tgggagtatt ccttccactg atcacaacaa actgtgcagt tcttggtaca gcattaacaa
     5581 acgtacagaa cgactatagt ttcatcgcca gcgtagtcaa tggtatcggt atctct
//
LOCUS       DAOMUE010000118         5590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_3978_length_5590_cov_2.337489, whole genome shotgun sequence.
ACCESSION   DAOMUE010000118 DAOMUE010000000
VERSION     DAOMUE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3978_length_5590_cov_2.337489"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="PLC71_06385"
     CDS             complement(<1..957)
                     /locus_tag="PLC71_06385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM02303.1"
                     /translation="MKLISFVVPCYNSQEYMRHCIDTLLPGGDQVEILIVNDGSSDNT
                     AAIADEYEKKYPNICRAIHQENKGHGGAVNTGIKNATGIYLKVVDSDDWVNEYAYGEI
                     LDTLANFYKQGTVLDMLLSNYVYEKQGAKKKTVIHYRHAIPKDQIFCWDDVGRFPVSQ
                     YILMHSVIYRTALLRECDLELPEHTFYVDNIYVFKPLPYVKTMYYLDVNFYRYFIGRD
                     DQSVNETNMIKRIDQQFKVTYIMIDYMTQFNLVSKPLERYMRHYLSIIMCISSVISVK
                     SGQKEHLKMRKELWKYLYDSDKRLYRKIRHNITSCAVNLPGYV"
     assembly_gap    1042..1060
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1061..>1600)
                     /locus_tag="PLC71_06390"
     CDS             complement(<1061..>1600)
                     /locus_tag="PLC71_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM02304.1"
                     /translation="PISSLSGGEKGRVSLAKLMLSHANFLILDEPTNHLDIQSKEILE
                     SAMNAYTGTILYVSHDRYFINKTATRILEISDHGLTSYPGNYQDYIAQKEKEKASLTS
                     STASSVSVDTESKLDWKQQKEEQAKQRKKENRLKKVEEQITELETKQEELSEAMSLPE
                     NASDPAKLMELSNEQASIAS"
     assembly_gap    1601..1610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1611..2972)
                     /locus_tag="PLC71_06395"
                     /pseudo
     CDS             complement(1611..2972)
                     /locus_tag="PLC71_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530232.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     gene            3125..3778
                     /locus_tag="PLC71_06400"
     CDS             3125..3778
                     /locus_tag="PLC71_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRM02305.1"
                     /translation="MANSDKNISSAVIKRLPRYFRYLGALKAKNITRISSKELSEQMM
                     VTASQIRQDLNNFGGFGQQGYGYNVDNLYTEIGKILGLDKRNDMIIVGAGNLGQALAN
                     YQSFNEDYGYKVMALFDVNPKLIGISVRGVKVLDTDELENFIKTHGIKIAALTIPKDQ
                     APAIAKKMAEWGIKGFWNFAPIDLHLPDNVTVENVHLAESLMTLTYNVHNREESENE"
     gene            3790..4152
                     /gene="acpS"
                     /locus_tag="PLC71_06405"
     CDS             3790..4152
                     /gene="acpS"
                     /locus_tag="PLC71_06405"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRM02306.1"
                     /translation="MIAGIGTDLVEIYRIEEMILRGRHLSRIFTTTELAYAKDNATTL
                     AGNFAVKEAVSKVFGTGFFGVEPYEIEVLRNESGAPYVKLYGKAKEIAKDKQVSRIHV
                     SITNTREFAQAFAIGECV"
     gene            4149..>5590
                     /locus_tag="PLC71_06410"
     CDS             4149..>5590
                     /locus_tag="PLC71_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRM02307.1"
                     /translation="MRYVLKNQEMQTVDHETIHGIGIPGLVLMERASKAVADKAAQIA
                     DHSQRILIISGIGNNGGDALACTRILLEQDYLVDYMIVGNIEKASADLKLQLQILKNL
                     GYFPIDCEDVNFHEYDLLIEGIFGVGLSREVGGVYKEVIERINACGKPVIAIDIPSGI
                     SGDSGKILGCAVHASSTVTFGGYKRGHLLYPGREYCGDIKLVSIGFHNQTIKKYATGY
                     ILEESDHLMPERKAYSNKGSYGKILIIAGNETMSGAACFAAEAVLRMGAGLVKVLSDA
                     GNRQILQTRLPEILFGERKDLEESLEWCDCILFGPGVGVSEEMKEMLEMVLKKGKKPL
                     VIDADGLNIISRFNMKIEYPYGTIMTPHLMEAARLMSCDITQIKEDLCQSAQDLAEKY
                     HCTAVLKDATTIVAREDDLVYINQSGNDGMATGGSGDVLAGMITGLLGEGLSVHEAAV
                     HGVYLHGLAGDHAKHDLGAYSMIASDIIAH"
BASE COUNT         1757 a          913 c         1136 g         1755 t
ORIGIN      
        1 aacatatcca ggcagattta ctgcacaact tgtaatatta tgacggatct tacgatacag
       61 acgtttgtca ctgtcatata aatatttcca taattctttt cgcatcttta aatgctcttt
      121 ctgtccagat tttacagaga tcacggaaga aatacacatg ataatggaaa gataatgtct
      181 catataacgt tctaatggct tacttacaag attaaactga gtcatataat caatcatgat
      241 ataagtaact ttaaactgct ggtcgatacg tttgatcata tttgtctcat tgacagactg
      301 atcatcacgt ccaatgaaat aacgatagaa gttaacatcc agataataca ttgtttttac
      361 atatggaaga ggtttgaata cataaatatt atccacatag aatgtatgct ctggaagctc
      421 tagatcgcat tcacgaagca gtgcagtacg ataaatgaca gagtgcatta aaatatactg
      481 gctgacaggg aaacgtccaa catcatccca gcaaaagatc tgatcttttg ggattgcatg
      541 acgataatga ataacagttt ttttcttggc tccctgtttt tcatatacat agttagaaag
      601 cagcatatca aggacagttc cctgtttgta gaaattcgca agagtatcaa ggatttctcc
      661 ataagcatat tcatttaccc agtcgtcact atcaacaact tttaggtaaa tacccgtggc
      721 atttttgatt cctgtattca ctgcaccacc atgcccttta ttttcctggt gaattgcacg
      781 gcagatgtta ggatattttt tttcatattc atctgcgatc gcagcagtgt tatcggaaga
      841 tccgtcatta acgatcagaa tctctacctg atcaccacct ggtaataagg tgtcaatgca
      901 gtgtctcata tattcctgag agttgtagca aggtacaaca aaggatatta atttcataaa
      961 aacctcctgt ttttcaatac ggttaacgtt actattatat atgttttttt acaaattagc
     1021 aaagagtttc ccattcttca tnnnnnnnnn nnnnnnnnnn agaagcgatg gaagcctgct
     1081 cattgctaag ttccattaat ttcgcaggat cagaagcatt ttcaggtaaa gacatagctt
     1141 ctgaaagttc ttcttgtttc gtttccaact ctgtgatctg ctcttcgact tttttaagac
     1201 gattttcttt ttttctctgt ttggcttgtt cttctttttg ctgtttccaa tcgagtttgg
     1261 attctgtatc aacagaaacg gaggaagcag tagaagaagt aagagatgcc ttttcttttt
     1321 ccttctgagc aatataatcc tgataatttc caggataaga agttaatcca tgatcagaga
     1381 tttccaagat tcgtgttgca gttttattga taaaataacg atcatgtgaa acataaagga
     1441 tcgttccagt ataagcattc attgcagatt ccagaatctc cttagactga atatctaaat
     1501 ggtttgtagg ctcatcaaga atcagaaaat tcgcatgaga cagcattaat tttgcaagag
     1561 aaacacgtcc tttttctcca ccgcttaaag aggagatcgg nnnnnnnnnn tctccataaa
     1621 ataaaaatgc agccaaagta ttgcggatct ttgtgttatt aagatctgga taagcatctg
     1681 aaatttcatc aaaaatcgtt ttggaatcat ctaagttatc ttgtgcctgg tcatagtatc
     1741 caatggaaac tccagctcca aggctgatcg taccgccatt attgctgatt tttttgcaaa
     1801 tgatctttaa cagtgttgtt ttaccagcct ttggcggtga tacaatcata ccacgctggc
     1861 cttttcccac agggctcatc agttccgttt ggtccgatca gggcaacgcg ttcttcacga
     1921 aaaatatcag cacttaatcc atggaataat gatttgtttc caaaagattt ggatacatct
     1981 tttaaagtca gaacatcatt cccactgatg atctctggtt caatgctaag acgcatatca
     2041 gcattatatt ccgtaggttt ctccaataca tcaatcttat ttaaagcttt ttcacggctt
     2101 tctgcacgtt tgattgattt ttcacgattg aactgtttta attttttgat aacttcttgt
     2161 tgatgtttga tctcttgttg ctgatttttg tattctttta aacggattgc tttgatctga
     2221 tcacgttttt cgataaatgt tgtataattt ccatcaaaca gcatggagtg tccttggtcg
     2281 atctccatga ccttattgac aatcttatca agaaaatagc ggtcatgaga tacgatgatc
     2341 acagctcctt tgtaatgaga caggtaattt tcaagccacc ttatggaatc aacatccaag
     2401 tggtttgttg gttcgtcaag aagcagaata tcaggatttt taagcagtaa cttaccaaga
     2461 gcaacacgag ttctttgacc tccagataaa gtgttgatct cttttgtgaa atcttcttca
     2521 gaaaagccaa gtccttttaa gattcctgtg atctcacttt tgtatgcaaa accatttaat
     2581 tgctcaaatc tatgagtagc atttgtatac tggttcattt tttgttctag tgcatcaccg
     2641 gtcagatgtt tcatttcctg ttcataggta cgcatctgtt catttaattc aatgacttct
     2701 tttttgacgg aaagaagttc atcaaaaatt gtatgatgga atgtatgatc ttgatgttgg
     2761 gcaagatagc caatgcttgc gtctttcttt aaagaaattt caccttcatc ataagagatt
     2821 tcaccaatca atatttttaa aagtgtcgat tttccggcac cgttatatcc gatgatcgcc
     2881 agccggtcgt tttcatttaa gtgaaatgag atattctgca agactggttt ttcgacaaat
     2941 gttttgcaga tattctggca tgataatatc attaagatac ccctttcaaa ttttttatta
     3001 tagacattat ttctcagaat accatatttt ttgaaaaaat gaaatatttt catgacggaa
     3061 agattgacag ttattagaca atcttatata attataagcg aaataaagat tttggaggaa
     3121 taaaatggca aactcagata agaatatttc ctcagcagtt atcaaacgac ttcctaggta
     3181 tttcagatat ttaggagcgt taaaagcgaa gaatattaca agaatctctt caaaagaatt
     3241 aagtgaacag atgatggtaa cagcatctca gattcgtcag gaccttaaca attttggtgg
     3301 atttgggcag cagggatacg gatataatgt agataatctg tatacagaga ttggtaagat
     3361 cttaggtctg gataagagaa atgacatgat cattgttgga gccggaaatc ttggacaggc
     3421 ccttgcgaat tatcagtctt ttaatgaaga ttatggatac aaagtcatgg cactttttga
     3481 tgtgaatcca aagctgatcg gaatttctgt ccgtggagtg aaagttcttg atacagatga
     3541 actagagaac tttatcaaaa cacatggaat taagatcgct gcactgacaa ttccgaagga
     3601 tcaggcacct gcgatcgcaa agaagatggc agaatgggga attaaaggat tctggaattt
     3661 tgcaccgatc gatcttcatt taccagataa tgttacagta gagaatgtac atttagcaga
     3721 aagtctgatg acattaacat acaatgtcca caaccgtgaa gagagtgaga acgaatagca
     3781 ggtaagaaga tgattgctgg aataggaaca gatctcgtag agatataccg gatagaagag
     3841 atgattcttc gaggaagaca tctttcaaga atttttacga caactgaatt agcatatgca
     3901 aaagacaatg ctacgacttt agctggcaat tttgctgtaa aagaagccgt ttccaaagta
     3961 tttggaaccg gctttttcgg tgtggaacca tatgagattg aagtgttgag aaatgaatca
     4021 ggtgcgccat atgtcaaatt atatggaaag gcgaaagaga tagcaaaaga taaacaagtt
     4081 tcaagaattc atgtatcgat cacgaataca agagaatttg cacaagcttt tgcgatagga
     4141 gaatgcgtat gagatatgta ttaaagaatc aggagatgca aacggtcgat catgagacga
     4201 ttcatggaat aggaattccg ggacttgtat tgatggaaag agcatctaaa gcagttgcag
     4261 ataaggctgc acagatagca gatcattcac agaggatatt gatcatttca ggaattggca
     4321 ataatggagg agatgcactt gcttgtacga gaattttgtt ggagcaggat tatttagtgg
     4381 attatatgat cgttggtaat atagaaaaag cttcagcgga tctgaaatta cagcttcaga
     4441 tacttaagaa tctaggctat tttccgatag attgtgaaga tgtaaatttt catgaatatg
     4501 atcttttgat cgaagggatt tttggagttg gactttctag agaagttggt ggtgtttata
     4561 aagaagtcat tgaacggatc aatgcatgtg gtaagcctgt gatcgcaatt gatattccat
     4621 ctggaatcag tggagatagt ggaaagattc ttggatgtgc agtgcatgcg tcatcaacag
     4681 ttacatttgg tggatataaa agaggtcatt tattatatcc aggacgagaa tattgtggag
     4741 atattaaact ggtatccata ggatttcata accagacgat caaaaagtat gcgacaggat
     4801 atatattaga ggaaagtgat catttaatgc cagaaagaaa agcatattcc aataaaggaa
     4861 gctatgggaa gattttgatc attgctggaa atgaaacaat gtcaggagct gcatgttttg
     4921 cagcagaagc agtacttcgg atgggtgcag gacttgtgaa agtgctttct gatgctggaa
     4981 atcgtcagat tttacagaca agacttccag aaattttatt tggagaacga aaagatttag
     5041 aagaatcttt agaatggtgt gattgtatct tgtttggccc aggagttggt gtcagtgaag
     5101 aaatgaaaga aatgttagag atggttttga aaaaagggaa aaaaccattg gtcattgatg
     5161 cagacggatt aaatataatc agtagattta acatgaaaat tgaatatcca tatgggacga
     5221 tcatgactcc acatttgatg gaggcagcaa gattgatgtc atgtgatatc acacagatca
     5281 aagaagatct atgtcagagt gcacaagatc ttgcagagaa atatcattgt acagctgttt
     5341 taaaagatgc aacaactatc gttgcacgag aagatgattt ggtgtatatt aatcaaagcg
     5401 gaaatgatgg aatggcgact ggaggaagtg gagatgttct tgcgggaatg atcacaggcc
     5461 ttttggggga aggattatct gttcatgagg cagcagttca cggagtttat cttcacggtc
     5521 ttgcaggaga tcatgcaaag catgatctgg gcgcttacag catgatcgca tcagatatta
     5581 ttgctcatat
//
LOCUS       DAOMUE010000119         5453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4114_length_5453_cov_3.007410, whole genome shotgun sequence.
ACCESSION   DAOMUE010000119 DAOMUE010000000
VERSION     DAOMUE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4114_length_5453_cov_3.007410"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..783)
                     /locus_tag="PLC71_06415"
     CDS             complement(85..783)
                     /locus_tag="PLC71_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM02308.1"
                     /translation="MNKALILVVEDDRPIRNLIVTTLKTHDYKYITAENGSSAILEAS
                     SHKPEIILLDLGLPDMEGVDVIKKVRTWSNVPIIVISARSEDVDKIEALDAGADDYIT
                     KPFSVDELLARIRVTQRRLALIQSGEEQEQAVFVNGELKIDYTAGSTYLKDKELHLTP
                     IEYKLLCLLSHNVGKVLTHTYITQQIWGSSLENDVASLRVFMATLRKKLQPDKDGMQY
                     IQTHIGIGYRMLKI"
     gene            complement(776..2752)
                     /locus_tag="PLC71_06420"
     CDS             complement(776..2752)
                     /locus_tag="PLC71_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRM02309.1"
                     /translation="MSTGEFVELNKENEHILVCLSSSPSNEKIIRTAAKMARDMRARF
                     TALYVQTGKRERESDKRRLEAHVQFAKESGAEIVMTHGENVPVQIAEYAHLSNVTKIV
                     IGQSSAKRNHFFSKQTLTEKLIEAVPDIDIHIIPDAMNLDNYRKPHGAVYVGKPTMKD
                     SLLTLFIFAVCTLIGLFIQKLQFTDTNIVTIYILGVLLTSILTDGYLYSIGGSVLSVF
                     LFCFFLTEPRMSFQTYAVGYPVTFVIMLISSVITGTLAAKLKTHARLSAQSAFRTQIL
                     FDTDRLLQKAKNDTDILSITCTQLIKLLDRSIVAYVVEDDELSAPHIFSNKKKEQTED
                     LLTSREQEIAKWVYENKQRAGATTERFKDAQCLYLAICIEDNVYGVIAIPVDEYTLDS
                     FEYSILLSVINECALAMENKKNIMEKEKISVLAKNEQLRADLLRAISHDLRTPLCSIS
                     GNADMLLNSGERLDDITKHQIYTDIYDDSEWLINIVENLLSITRLNDGRLKLKFTDQL
                     LDEVIAESLRHISRKHEEYQIVTECDELILAHMDVRLILQVLINLVDNAIKYTPKGST
                     IRICAMNENGKAKIQVEDNGPGICDEMKPHIFEMFYTGKNTIADSHRSLGLGLALCRS
                     IIEVHNGKLVLEDNTPHGCIFSFTLPLSEVTLNE"
     gene            complement(2816..2950)
                     /locus_tag="PLC71_06425"
     CDS             complement(2816..2950)
                     /locus_tag="PLC71_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02310.1"
                     /translation="MSDFILALAVILMFAFVLFFLKKVNYFLGENRKVIDREQDDDDD
                     "
     gene            complement(2976..3623)
                     /locus_tag="PLC71_06430"
     CDS             complement(2976..3623)
                     /locus_tag="PLC71_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRM02311.1"
                     /translation="MKNILLIGLGRFGKHIAIQSSQLGHEIMAVDLDEERVNDALPYV
                     TNAQIGDSTNEEFLKSLGIANYDICFVTIGGNFQNSLETTCLLKELGGKMVISRAERD
                     VQEKFLLRNGADKVVYPEKMVAKWATIRYTDDHILDYMEVDASHAIFEVEVPKEWIGK
                     TVGKLDIRKKYDINIMAVKDDGKVSMAISADTCFKEDVTLLVLGEYKAIKKCFCI"
     gene            complement(3643..4980)
                     /locus_tag="PLC71_06435"
     CDS             complement(3643..4980)
                     /locus_tag="PLC71_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRM02312.1"
                     /translation="MPWKIHKKRHLTSFQVIILGFAGVILFGALILMLPISSAQAIIT
                     PFHKALFTSTSAVCVTGLAVLDTGSYWSVFGQTVIMLLIQIGGLGVITTATAVFILSG
                     RKISILQRSTMQNAISAPKVGGIVRLMSFILKGTLLIELIGALFMMPVFCHDFGLRGI
                     WMSIFHSVSAFCNAGFDLLGTKWHPFVSLTSYAVNPVINIVIMLLIIIGGIGFFTWED
                     IYLHKFHFKHYHMQSKVILTTTLCLILLPAVFFFFQDYGNLSGIHRLLASLFQAVTPR
                     TAGFNTTDLSMLTGASKAMMILLMLIGGSPSSTAGGMKTTTFALLLLNVLATFQSCDD
                     VTAFGRRIDASVIKNATTIAMMYFILFFAGGMTISVYEGLPLSSCLFEAASAVGTVGL
                     TLGITPKLHILSQIILIILMYLGRVGGLTLIYAVFSDKNKRKARLPLDKIIVG"
     assembly_gap    5185..5222
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1594 a         1156 c          835 g         1830 t
ORIGIN      
        1 ttttttgctt cttttgggcg ttttcatata ttggaatcta aaagagtgtc tgcatcactt
       61 aatgatgcag acactcttta tttactatat cttaagcatc cgatatccaa tacctatatg
      121 tgtctgtata tactgcatgc catctttatc tggctgtaat ttcttccgta atgttgccat
      181 aaacactcta agtgatgcaa catcattttc cagtgaactg ccccagatct gttgcgtgat
      241 ataagtgtga gtcaatactt ttccaacatt atgagacagc aaacataata atttatattc
      301 gattggtgtt aaatgcagtt ctttatcttt taaatatgta gatcctgctg tatagtcgat
      361 ctttaactct ccatttacaa agactgcttg ttcttgttcc tcgcctgact ggatcaatgc
      421 taatcggcgc tgtgtaaccc tgatccttgc aagcagttca tcaactgaaa atggttttgt
      481 gatataatca tctgctcctg catccagtgc ttctatttta tctacatctt cacttcttgc
      541 actgataacg ataattggta cgttagacca tgttcttact tttttgatca catccacacc
      601 ttccatatca ggcaatccaa gatctaataa aatgatctct ggtttatgag aagatgcctc
      661 taaaatagct gaacttccat tttctgctgt aatatattta taatcatgtg tttttaatgt
      721 tgtcacgatc agattccgga tcggccggtc atcctccacg actaaaatta atgctttatt
      781 catttaatgt aacctcactt aatggcagtg tgaaagaaaa aatacaacca tgtggtgtat
      841 tgtcctccaa caccagtttc ccgttatgca cctctatgat cgaacggcaa agtgccaaac
      901 ctaaaccaag acttcgatgg ctgtctgcga tcgtattttt ccctgtataa aacatttcaa
      961 atatatgcgg cttcatttca tcacaaattc cagggccgtt atcttctact tgaatctttg
     1021 cttttccatt ttcattcatg gcacaaatgc ggattgtaga tcctttcggt gtatatttaa
     1081 ttgcattatc gaccagattg atcaacactt gcaggatcag ccgcacatcc atatgtgcga
     1141 ggatcaattc atcgcattct gtaacgatct gatactcttc atgtttcctg ctgatatgcc
     1201 gtaacgattc tgcgatcacc tcatctaata attgatctgt aaatttcagt tttaatcttc
     1261 catcatttaa tcttgtgatc gataataaat tttcaacgat attgatcagc cattctgaat
     1321 catcataaat atctgtataa atctgatgtt ttgttatatc atcaagacgt tctccgctat
     1381 ttaagagcat gtctgcattc ccagaaatgg aacaaagtgg tgtccttaga tcatgtgaga
     1441 tcgcacgaag cagatctgct cttaactgtt catttttggc aagaacggag attttttctt
     1501 tctccatgat attcttttta ttttccatgg caagtgcaca ttcattgatc acagaaagta
     1561 agatactata ttcgaaggaa tctaatgtat attcgtcaac tggtattgcg atcacaccat
     1621 acacattatc ctcaatacaa atggcaaggt atagacactg tgcatcttta aaacgttcgg
     1681 tcgttgctcc agcacgctgc ttgttttcat acacccattt tgcgatctcc tgctccctgc
     1741 ttgtcaataa atcttctgtc tgttctttct ttttattcga aaatatatgt ggtgctgaca
     1801 attcatcgtc ttccacaaca tatgctacaa tactgcggtc taataatttg ataagctgtg
     1861 tacatgtaat acttaaaata tccgtatcgt tttttgcttt ttgaagtaac cggtctgtgt
     1921 caaataaaat ctgtgtacga aatgcagatt gtgctgaaag ccttgcatgt gtttttaatt
     1981 ttgctgcaag tgttccagta atgacagaag atattaacat gatcacaaaa gttaccggat
     2041 atccaactgc atatgtctga aatgacattc ttggttccgt aaggaaaaag cagaacaaaa
     2101 atacacttaa gacagaacct ccaatgctgt ataaatatcc atctgtaagg attgacgtta
     2161 ataaaacacc caaaatataa attgtaacga tgtttgtatc tgtaaattga agtttctgga
     2221 tgaatagacc gatcaacgta caaactgcaa atataaataa tgtcaataaa ctgtctttca
     2281 tcgtaggctt tcctacataa accgctccat gcggttttcg ataattatct aagttcatcg
     2341 catctggaat aatgtggata tcaatatcag gaacagcctc gatcaacttc tctgttaatg
     2401 tctgtttgct gaaaaagtga tttctctttg cactgctttg tccgatcacg atttttgtga
     2461 catttgataa atgtgcatat tccgcgatct gtaccggaac attttcccca tgtgtcatta
     2521 cgatttccgc accagattcc tttgcaaatt gtacatgagc ttccagtctt cttttgtccg
     2581 attctctttc tctttttcct gtctgtacat acaatgccgt aaaacgtgcc cgcatatctc
     2641 ttgccatttt tgctgctgta cgtatgatct tttcattcga tggtgaagaa gaaagacaaa
     2701 caagaatgtg ttcgttttct ttgttcagct ctacaaattc tcctgtgctc atcttggccc
     2761 cctttcctta tcggatgcat tatatcacga tcgtcaataa taatccataa acacatcaat
     2821 catcatcatc atcttgctcc ctgtcaatta ctttccgatt ttctccaaga aaataattta
     2881 cttttttcaa aaagaataac acaaatgcaa acatcagtat taccgccaat gctaaaatga
     2941 aatctgacat aagctctcac ctctttttca tgacatcaga tacaaaaaca ctttttgatc
     3001 gctttatatt ctcctaatac caataatgta acatcttcct taaaacaggt atccgcagat
     3061 atcgccatac ttacttttcc atcatctttt actgccataa tgttaatatc atattttttt
     3121 ctaatatcaa gttttcccac tgtctttccg atccattcct tcggaacctc tacctcaaag
     3181 atcgcatgtg aggcatcgac ttccatataa tcaagaatat gatcatcggt ataacggatc
     3241 gttgcccatt ttgccaccat tttttctgga tagactacct tatcggctcc atttcgtaag
     3301 aggaattttt cctgtacatc tcgttcagct ctggaaataa ccattttgcc tccaagttct
     3361 tttaacagac aggtggtttc caacgaattt tgaaaattcc caccgatcgt aacaaaacag
     3421 atatcataat ttgcaatccc cagagatttt aaaaattcct cattcgtact gtccccgatc
     3481 tgtgcattcg taacatatgg aagtgcatca ttcactcttt cctcatccag atcaactgcc
     3541 atgatctcat gtccaagctg acttgactgt atcgctatat gttttccaaa acgtccaagt
     3601 ccgatcaata aaatattctt catcatcaat tccctctttc ttttatccca ctataatctt
     3661 atcaagtggt agtcttgctt ttcgtttatt tttatctgaa aatactgcat agatcagcgt
     3721 caaaccgcca acacgtccca gatacatcag tatgatcaaa atgatctgtg acagaatatg
     3781 aagttttggt gtgattccta atgtcaaacc tacggttcca actgcagatg ctgcttcaaa
     3841 cagacagctt gaaagcggca gtccctcata cacactgatc gtcattccgc ctgcaaaaaa
     3901 caagataaaa tacatcattg cgatcgttgt tgcatttttt atcacactag catcgattct
     3961 tcttccaaat gctgtcacat catcacaact ttgaaaggtc gccagtacat ttaaaagtaa
     4021 cagagcaaat gttgttgttt tcatacctcc tgctgtagaa cttggtgaac ctccgatcaa
     4081 catcaataaa atcatcattg cctttgatgc tcctgtcagc atcgaaagat ctgtcgtatt
     4141 aaatcctgca gttctcggtg tcacagcttg aaacaatgat gcaagcaacc ggtgaatccc
     4201 tgatagattt ccataatcct gaaagaaaaa gaaaacagct ggtaacagga tcagacataa
     4261 tgttgtagtt agaataacct tactctgcat atggtaatgc ttaaaatgaa atttatgaag
     4321 ataaatgtct tcccatgtaa aaaatccaat tccaccaatg atgatcagta acatgatcac
     4381 aatgttgatc actggattta ccgcatatga tgtcaatgat acaaatggat gccattttgt
     4441 tccaagaaga tcaaatcctg cattacaaaa ggctgataca gaatgaaaga tcgacatcca
     4501 gattccccgt aatccaaaat catgacagaa aacaggcatc atgaataacg caccgatcaa
     4561 ttcgatcaat aaagttcctt ttaaaataaa actcatcaat cgaacgatac cccctacttt
     4621 cggtgcagag atcgcatttt gcatcgtact tcgctgcagg atcgatattt ttcttcctga
     4681 taatataaat acggctgttg ctgttgttat aactcccaga cctccgatct gtatcagtaa
     4741 catgatcact gtctgtccaa aaacggacca gtaacttcca gtatccagaa ctgcaagccc
     4801 tgtcacacat actgcagaag ttgacgtaaa taatgctttg tgaaatggtg ttatgattgc
     4861 ttgagctgat gaaattggca gcatcaggat caatgctcca aaaaggatca ctcctgcgaa
     4921 ccctaatatg atcacctgaa atgatgttaa atgtcttttt ttatgaatct tccatggcat
     4981 atatgatctt acctcttttt caaattattt tatgactttt tgatcttatc atcattatat
     5041 cttttatgat aaaaagatag tgtaaggata tgttctcttg tattaagatt gtataaagaa
     5101 aaaaggagca taatatgatt tcaagaaagg atcctgcttt acaggattct ccgcctgcgg
     5161 cgggtcgctt ctgcgacata ttacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnaaaaatat ctgtaaaata taaaatccat gagaacacga ttttttggtt tgttgttctc
     5281 atggatttta ttgtttctta ttttttcaag tattcttcaa tcatacattc atgcttcttt
     5341 tcttttttac tataattaat tcccacaagc aaaacattac ctgcatattt ttgaatagaa
     5401 tctggatatt ttttatcctt aatctgttgc aatgcactaa cagcactctt att
//
LOCUS       DAOMUE010000120         5452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4116_length_5452_cov_2.156754, whole genome shotgun sequence.
ACCESSION   DAOMUE010000120 DAOMUE010000000
VERSION     DAOMUE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4116_length_5452_cov_2.156754"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="PLC71_06440"
     CDS             <1..1278
                     /locus_tag="PLC71_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM02313.1"
                     /translation="GSLLTIFYTDYVGISAAAIGVIMLISRIFDGCSDVIMGFVVNKT
                     HSKYGQSRPWILWMAIPYAVSAVLLFTVPHTATNIQLIYIFVTYNFTATVCYTALNLP
                     YGSLSAMMTRSSRERDMLSIVRMALSPIGRIMAVTFTTPLVKLFGDDQAAWVKTMAIW
                     AVLALLMLITCFKNCEEKVKIKVVKKEKIPVGKTFSALLKNQYFWAVLILWMMQNVIT
                     SITGTILPYYCKYILGNDTWMYSVLYMVETVTLIAATLCSPLLLKRLGKRNMSLIGCI
                     GCLIGQFVFMVKPESFYWLLGCCIIRGICFAPLNSVIFGMLGDVVEFGQWKTHLRQES
                     FIFAIGSVGTKLGNGITAAVLTQLLAFSGYVSKAGVTTQPQSVVNMIMNIYKVGPLIV
                     WVTVVIILLLYRLDKKYPMIMKELEEREARGEL"
     gene            1323..2222
                     /gene="iolE"
                     /locus_tag="PLC71_06445"
     CDS             1323..2222
                     /gene="iolE"
                     /locus_tag="PLC71_06445"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HRM02314.1"
                     /translation="MFDKNKVKLGIAPIAWTNDDLPDLGAENTFEQCVSEMALAGFTG
                     SEVGNKYPKDPAELKKALDLRGIEICNQWFSSFLITKPLEEVEKEFRAQLEFLKAMGA
                     KVIGASEQSYSVQGQEDTPVFGHKYVMDDEEWDTFCNGMNYLGKIAKEEYGIALTFHH
                     HMGTVVQDPDEVERMMANTDPEYVSLLFDTGHFTYCGADPLEMVKKYVNRIKHVHLKD
                     IRPEVVKEVKDNDLSFLEGVRRGAFTVPGDGCIDFDPIFKVLEENGYEGYMLVEAEQD
                     PAKANPLEYAIKARKFIREKTGL"
     gene            2244..2843
                     /locus_tag="PLC71_06450"
     CDS             2244..2843
                     /locus_tag="PLC71_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRM02315.1"
                     /translation="MGNTLITISRQHGSGGTKVAQILAEKLGIWYYNREILYIAADKI
                     GYDSLNEKSMDEINYKKASKYMEGLSIMTGSRNHIPVYNQMFKEQSKIIKKLAGYGSG
                     VFLGRCADYVLKDEPGVCSVYLYAEESYRIKHLSEKEGRAVTAEEIRKEDKMRSSYYS
                     YYTGRNWYDMENYDLAINLATIEAEEAAQLILDYMEKRK"
     gene            2940..4151
                     /locus_tag="PLC71_06455"
     CDS             2940..4151
                     /locus_tag="PLC71_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM02316.1"
                     /translation="MKKRNGMMAAGAFAILFTGFPHIWSIYQPYVLSETGWNESMVSI
                     CFYLPTLFFVVGNIFGGRIYDKGYERMTLGIGGSVFAGGIILSALTLGIHPMITYMML
                     GVMQGIGQGMIYAVVLATAQKWFPHKTGFASGVVITANGLCGFILSPVSKLLLERGGV
                     RLTLVSVGFLVFLSAVCAVCFVRQPEAENIETKENQIYAGQQYTSAQMMRTKKFYYLV
                     ATMMCGLMPYYLVSPISQTIQIERGILQAVAVGSVMAGSILNAGIRLILPTLADRAGR
                     ITCIQGVLFVAMIAMLFLLSGNNRLITMAIILAYGCFGGIMGSFPSLSSEVFGLRYAG
                     QNYGIIMSGIIIVTITSPVISNMILIHELSEVVRFMTGFCFAVIALGFSMLLKKEINQ
                     SGKEVKNYGTC"
     gene            4138..4974
                     /locus_tag="PLC71_06460"
     CDS             4138..4974
                     /locus_tag="PLC71_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HRM02317.1"
                     /translation="MALVKMKDLLLRAQKENKGCGAFSVGNMEMVKGAIKAAEELDTP
                     IILQIAEVRLDHSPLHLMGPMMLEAARNAKVDVAVHLDHGLKFETIKKALEYGFTSVM
                     LDASRDSFEENIEKTKKVVELAAKYGATVEAELGLVGGSEDGKSDHGIRCTNPEDAKI
                     FCEETGIDALAVAIGNAHGDYPVAPQLEFGVLEEIYNRTGIPLVLHGGSGITDADFQK
                     AISLGIRKVNIATASFKSLTKEVENYLATEGAHNYFDMNEAMVLGTYKNVKHHIEVFN
                     CK"
     gene            5097..>5452
                     /locus_tag="PLC71_06465"
     CDS             5097..>5452
                     /locus_tag="PLC71_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM02318.1"
                     /translation="MAVTMQQIADLAGVSRGTVDRALNDRGRIRPEVAEKIKMIAKEL
                     GYQPNIAAKALSSGNQILRIGVILQYADTPTIQTVLEGAKDAKQEVERFGGRVMIDQI
                     NGGDVRQVLDIMRGYK"
BASE COUNT         1894 a          829 c         1262 g         1467 t
ORIGIN      
        1 ggaagcctgt taacgatctt ttatactgat tatgttggaa tcagtgcagc agcgattggt
       61 gtgatcatgc tgatatccag aatttttgat ggatgttctg atgttatcat gggattcgtt
      121 gtcaataaaa cacacagcaa atatggtcaa tccagaccat ggatcttatg gatggcgatt
      181 ccatatgcag tatcggcagt attattattc acagtaccac atactgcaac taacatacag
      241 ttaatatata ttttcgtaac ttataatttt acagcaacgg tctgctatac agcgttgaat
      301 cttccatacg gaagtttatc tgcgatgatg acaagatctt ccagagaaag agatatgtta
      361 agtatcgttc gtatggcact ttctccgatc ggaagaatca tggcagttac atttacaaca
      421 ccactggtta aattatttgg ggatgaccag gcagcgtggg taaagacaat ggcaatctgg
      481 gcagtcttag cactcctaat gctgatcacg tgttttaaaa actgtgaaga aaaagttaag
      541 atcaaagtag tgaaaaaaga aaagatccct gtaggaaaga cattttcagc attactgaaa
      601 aaccagtatt tctgggcagt actgatctta tggatgatgc agaacgtcat cacatcgatc
      661 acaggaacga tccttccata ctactgtaag tatattcttg gaaatgatac atggatgtac
      721 agtgtattat acatggtaga aacagttaca ttgatcgcag ctactttatg cagtccgtta
      781 ttattaaaac gacttggaaa gagaaatatg tccttgatcg ggtgtatcgg atgtctgatc
      841 ggacaattcg tattcatggt caagccagag agtttttatt ggttacttgg atgttgtatc
      901 atcagaggaa tctgttttgc accattaaac tctgtaatct tcggaatgtt aggagatgtt
      961 gtagaatttg gtcagtggaa aacacattta cgtcaggaga gtttcatctt cgcgatcgga
     1021 tctgttggta caaagcttgg aaacggaatc acagctgcag ttttaacaca gttattagca
     1081 tttagcggat atgtcagcaa agctggtgtt acaacacagc cacagtcagt tgttaatatg
     1141 atcatgaata tctataaagt aggaccattg atcgtatggg taacagtcgt tattatctta
     1201 cttctgtaca gattggataa aaaatatcca atgatcatga aagaattaga agagagagaa
     1261 gcaagaggcg aattataaaa attttgcaca gtgcgtgctc gaacacaaga aaggaataag
     1321 agatgtttga taagaataaa gtaaaattag gaattgcacc aatcgcatgg acaaatgatg
     1381 atcttccaga tcttggagct gaaaatacat ttgaacagtg tgtcagtgaa atggcacttg
     1441 caggatttac agggtcagaa gtaggaaata aatatccaaa agatccagca gaattaaaga
     1501 aagcattaga tcttcgtgga atcgaaattt gtaatcagtg gttttcatct ttcctgatca
     1561 caaaaccatt agaggaagta gaaaaagaat tccgtgcaca gttagagttc ttaaaagcaa
     1621 tgggagcaaa agtgatcgga gcttctgaac aaagttacag tgtacaggga caggaagata
     1681 caccagtttt tggacataaa tatgtgatgg acgatgaaga atgggataca ttctgcaacg
     1741 gaatgaatta tcttggaaag attgcaaaag aggaatacgg aatcgcatta acattccatc
     1801 atcatatggg aacagtcgta caagacccag atgaagtaga acgcatgatg gcaaatacag
     1861 atccagaata cgtaagttta ttattcgata caggacattt tacttactgt ggagcagatc
     1921 cattagaaat ggtgaaaaaa tatgtaaacc gtattaaaca tgtgcattta aaagatatcc
     1981 gtccagaagt tgtaaaagaa gtgaaagaca acgatttaag cttccttgaa ggagtaagaa
     2041 gaggtgcatt tacagtacct ggagatggat gtattgattt tgatccgatc tttaaagtat
     2101 tagaggagaa tggatatgaa ggatatatgc ttgtagaagc agaacaagat ccagcaaaag
     2161 caaatccatt agaatatgcg atcaaagcac gtaaatttat cagagaaaaa actggattat
     2221 aaaccaggag aaagaggcat attatgggga atacattgat cacgatcagc cgacagcacg
     2281 gaagcggcgg aacaaaagta gcacagattt tggcagagaa attaggaata tggtactaca
     2341 acagagagat tctttatatt gccgcagata agattggata tgattctctc aatgaaaaga
     2401 gcatggatga gattaattat aaaaaggctt ccaaatatat ggaagggctt agtatcatga
     2461 caggttcaag aaatcatatt ccagtttata atcagatgtt caaagaacag tctaagatca
     2521 taaagaaatt agcaggatac ggatctggcg tcttcttagg aagatgcgca gattacgtat
     2581 taaaagatga accaggagtg tgctccgtgt atctttatgc agaggaatct taccgtataa
     2641 agcatctgtc agaaaaagaa ggaagagccg taacagcaga agaaatccga aaagaagata
     2701 agatgcgttc atcatactac agttattata ctggaagaaa ctggtatgat atggaaaatt
     2761 atgatcttgc gatcaatcta gcgaccattg aagcagaaga agctgcacaa ttgatccttg
     2821 attatatgga aaaaagaaaa taaactccaa attgattgac aacattacaa gaaagtgcta
     2881 aattattatc aaaagataac caataaatgc tcaatagata atcaagaagg agaaataaga
     2941 tgaaaaaaag gaatgggatg atggcagcag gagctttcgc gatcttgttt actggatttc
     3001 cacatatatg gtcaatttat cagccatatg tactctcgga gacaggctgg aatgaaagta
     3061 tggtatcaat ttgtttttat cttccaactt tgttttttgt agttggtaat atatttggtg
     3121 gaagaatcta tgataaagga tacgaaagaa tgactctcgg aatcggaggg agcgtatttg
     3181 caggtgggat catattatca gcattgactt taggaattca tccaatgatc acttatatga
     3241 tgctaggtgt catgcaggga atcggacaag gaatgatcta tgcagttgta cttgcaacag
     3301 cacagaagtg gtttcctcat aagacagggt ttgcttcagg agtagtgatc acagcgaatg
     3361 gattatgtgg atttatctta tctccagtca gtaaactctt attagaaaga ggtggagtaa
     3421 gactgacatt ggtgtctgtt gggtttctgg ttttcttatc agcagtttgt gctgtctgct
     3481 ttgtcagaca gccagaggca gaaaatatag aaacaaaaga aaatcagata tatgcaggac
     3541 aacaatatac atcagctcag atgatgagaa caaagaagtt ttattatctt gtagccacga
     3601 tgatgtgtgg tctgatgccg tattatctgg tgtcaccaat ctcacagaca attcagattg
     3661 agagaggaat cttacaggca gttgctgtag gttccgtgat ggcaggatct atattgaatg
     3721 cggggatcag actgatactt ccaacacttg cggacagagc aggaaggatc acttgtattc
     3781 aaggtgtttt gtttgttgca atgattgcta tgctcttttt actcagtgga aataacaggc
     3841 tcatcacaat ggcaattatc ctggcctatg gatgttttgg cggtattatg ggaagttttc
     3901 catcattgtc aagtgaggtt tttgggcttc gatatgcagg acagaattat ggaatcatca
     3961 tgtcagggat catcatcgta acgatcacct cgccggtcat atcgaatatg atactgatcc
     4021 atgaattgtc agaggtagta agatttatga caggattttg ttttgcagtg atcgcattag
     4081 gatttagtat gttattaaaa aaagagataa atcaatcagg aaaggaagta aaaaattatg
     4141 gcacttgtta aaatgaaaga tctgcttctt agagcacaga aagaaaacaa aggatgtgga
     4201 gcattcagtg taggaaatat ggagatggta aaaggtgcga tcaaggcagc agaagaactt
     4261 gatacaccga tcatccttca gatcgcagaa gtgagattag accattcacc actgcatctg
     4321 atgggaccta tgatgttaga agcagcaaga aatgcaaaag tagatgttgc ggtacatctg
     4381 gatcatggat taaaatttga gacgatcaaa aaagcattag aatatggatt tacatctgtt
     4441 atgctcgatg catccagaga ttcatttgaa gaaaatattg aaaaaacaaa gaaagtggta
     4501 gaacttgcag caaaatacgg tgccactgtt gaggcagaat tagggcttgt tggtggaagt
     4561 gaagatggaa aaagtgacca tggaatccgc tgtacaaacc cagaagatgc aaagattttt
     4621 tgtgaagaaa caggaattga tgcactggca gtagcgatcg gaaatgcaca tggagattat
     4681 ccagttgcac cgcagttaga atttggtgtg ttagaagaaa tctataatag aacaggaatc
     4741 cctcttgtgc ttcatggagg aagcgggatc acagatgctg atttccagaa agcgatctca
     4801 cttggaatcc gcaaagtcaa tattgcaaca gcaagcttta agagcttgac aaaagaagta
     4861 gaaaactatc tggcaacaga aggtgcacat aattactttg atatgaatga ggcaatggta
     4921 cttggaacgt ataagaatgt aaaacatcat atcgaggtat ttaactgtaa ataaatgata
     4981 aatgaaaaat gaaacaggta tgaggaaaag tgtaatacct gttttttatt tgcaaaaaat
     5041 aaaaaacgag ttataatata tgcgtgcacg ataacgcaaa gaaaggagaa caagaaatgg
     5101 cagtaacaat gcaacagatt gcggatctgg caggtgtatc aagaggaacg gtagacaggg
     5161 cattgaatga ccgtggacgt attcgaccgg aagtggcaga gaagatcaag atgattgcaa
     5221 aggaacttgg atatcagccg aatattgcgg caaaggcact atcgtcaggc aatcagatcc
     5281 tgcggatcgg agttattttg caatacgcag ataccccgac gatccagaca gtattagaag
     5341 gagctaagga tgcaaaacaa gaagtagaac gttttggcgg acgtgtgatg atcgaccaga
     5401 tcaatggtgg agatgtaaga caggtgttgg atatcatgag aggctataaa ga
//
LOCUS       DAOMUE010000121         5435 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4139_length_5435_cov_2.224349, whole genome shotgun sequence.
ACCESSION   DAOMUE010000121 DAOMUE010000000
VERSION     DAOMUE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4139_length_5435_cov_2.224349"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1031)
                     /locus_tag="PLC71_06470"
     CDS             complement(<1..>1031)
                     /locus_tag="PLC71_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HRM02319.1"
                     /translation="AGICHSFSSDGRCVSLLKILLSNECIYDCKYCLNRVSNDRVRTT
                     FTPDEICTLVVEFYRRNYIEGLFLSSGIVKSPTYTMELMYQAIYLLRTKYHFNGYIHV
                     KAIPGTSDELITNMGYLVDRMSVNLELPTIDGLKKLAPHKNPKKLVAPIRQIQNGIVD
                     HRLMIGKDASMERSQSNKYLKHTIFQENPYQRIQTGSSPLTLADPSLKNTLRLQNNGK
                     PASFVPAGQSTQMIIGATGESDLQLLSTTQKLYQQYDLKRVFYSAYVPLNEDSNLPAL
                     GTAPPLLREHRLYQADWLLRFYGFKADELLSVDRPNFNTMLDPKCDWALRHLDIYPVE
                     INRASYDMLL"
     assembly_gap    1032..1041
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1042..1160)
                     /locus_tag="PLC71_06475"
     CDS             complement(<1042..1160)
                     /locus_tag="PLC71_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02320.1"
                     /translation="MEISSKDLSLNEKLKILADAAKYDVACTSSGSSRRGKKG"
     gene            complement(1306..1656)
                     /locus_tag="PLC71_06480"
     CDS             complement(1306..1656)
                     /locus_tag="PLC71_06480"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HRM02321.1"
                     /translation="MKILCVTKCPTGMVQTYVAAEKLEQAGKKLGVDLSVETQGAMGI
                     QNQFSPEQIDEADYIVIAADVAIDEASRFGNKKLYVTSLEDAIVHPEQILESLTTKSY
                     SYQDAAVSNKKILG"
     gene            1868..2971
                     /locus_tag="PLC71_06485"
     CDS             1868..2971
                     /locus_tag="PLC71_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM02322.1"
                     /translation="MGKNKGEMKYHTLKESLKNDILSGVYQPGDKMPSENQLVQEYDL
                     SRHTVRKALSILEEEGYIIAVHGKGTFCTERALHKKESKNIAVVTTYISDYIFPRLIQ
                     GINSVLSKDGYSIIFKTTENSRRGETACLEDILTKDIDGLIIEPSKSEIVCRHPKLYE
                     NLDKYEIPYIFIHGVYEEMKDKPHILMDDCKGGYLLTKYLIDMGHQHIVGVFKADDHQ
                     GRERHKGYVMALQEAGYQYDPDMVVWFHTEDRQMKPVTSVVQMIKDQRKMDAVVCYND
                     QIAFSIIGGLHQIGVSIPEDLSITGYDNSMLAHQHPLSLTTIAHPQEKLGEMAGQLLL
                     EKIQGGSDKDMKTEYLIEPKLVIGNSCKNRNQE"
     gene            2994..4490
                     /gene="araA"
                     /locus_tag="PLC71_06490"
     CDS             2994..4490
                     /gene="araA"
                     /locus_tag="PLC71_06490"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HRM02323.1"
                     /translation="MELKKNYKFWFATGSQDLYGDECLSHVAKHAQIIVQNFNESGIL
                     PFEVVWKPTLIDSTSIRRTFEEANADEECAGVITWMHTFSPAKSWIAGLQAFKKPLLH
                     LHTQFNEEIPYDTIDMDFMNENQSAHGDREFGHIVTRMGIPREVVVGYYESKEVKEKI
                     ASWMRSAIGMVESKNIRVVRIADNMRNVAVTEGDKVEAQIKFGWEIDAYPVNEVVDYV
                     NAVSKGDIDALTQLYYEKYNLLLEGRDPIEFKKHVEVQAGIEIGLEKFLKDHDYQAVV
                     THFGDLGGLKQLPGLAIQRLMEKGYGFGAEGDWKTAAMVRLMKVMTAGKKDAKGTSFM
                     EDYTYNFVPGKEGILEAHMLEVCPTIADGPVSIKVNPLSMGDREDPARLVFTAKEGEG
                     IATSLIDLGHRFRLIINKVDCKKTEKPMPELPVATAFWTPKPDLYKGAEAWILAGGAH
                     HTAFSYDLSVDQMVAWAEAMGIESVVIDENTDIRILKNELRWNEVVYR"
     gene            4508..>5435
                     /locus_tag="PLC71_06495"
     CDS             4508..>5435
                     /locus_tag="PLC71_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRM02324.1"
                     /translation="METKQMIADGKTALGIEFGSTRIKVVLIDKDHHPIASGSHDWEN
                     RLENNIWTYTLEDIWSGLQDSYQNLVNDVKEKYDITLTKVGSIGFSAMMHGYMAFDKD
                     GELLVPFRTWRNTMTEEASEKLTEAFSYHIPQRWSIAHLYQAILKEEEHVKDIDYLTT
                     LAGYVHWKLTGEKVLGVGEASGMFPIDIETKDYNQTMIKTFDDLIKDKKFGWKLENIL
                     PKVLIAGDKAGILSEEGAKLLDPSGNLEAGIPLCPPEGDAGTGMVATNSVTRKTGNVS
                     AGTSVFAMIVLEKELKKVYDEIDLVTTPSGDLV"
BASE COUNT         1904 a          911 c         1156 g         1454 t
ORIGIN      
        1 agcaacatat cataagaagc acgattgatc tcaactggat aaatatcaag gtgacgtaac
       61 gcccagtcac actttggatc aagcatcgta ttaaaattcg gccggtctac agaaagtaat
      121 tcatctgcct taaatccgta aaaccgtagc agccagtctg cctgatacag acggtgttcc
      181 cgaagaagcg gcggtgcggt tccaagagct ggcagattag aatcctcatt caaaggaaca
      241 taagcagaat aaaacacacg tttcaagtca tactgctgat ataatttctg cgttgtcgat
      301 aaaagctgca gatcactctc cccagttgcg ccaatgatca tctgtgtact ttgaccggca
      361 ggaacaaaag aagctggttt cccattattt tgcaaacgta aagtattctt aagagaagga
      421 tctgcaagtg tcaatgggga agaaccagtc tgaatccgct gataaggatt ctcttgaaag
      481 atcgtatgct tcaaatactt attactttga gaacgttcca tagaggcatc ttttccgatc
      541 atcaaccgat gatccacgat tccattctga atctgacgaa ttggagcaac aagtttcttc
      601 ggattcttat gaggagccag tttcttaagc ccgtcaatcg taggaagttc cagattcaca
      661 ctcatacgat cgacaagata tcccatattc gtgatcaatt catccgaagt gccaggaatc
      721 gctttcacat ggatatatcc attaaaatgg tactttgtac gaagcagata aatcgcttga
      781 tacattaatt ccatcgtata agtcggactc tttacgattc cagagcttaa aaataaccct
      841 tcaatataat ttcttcgata aaattccaca acaagtgtac agatctcgtc cggagtaaat
      901 gttgtacgaa ccctgtcatt acttactcgg tttagacaat acttacaatc ataaatacat
      961 tcattgctaa gtaagatctt aagcaaagac acacagcgcc catccgaaga aaaactatga
     1021 cagattccag cnnnnnnnnn nattcctttc tttccacggc gggaagaacc actggaagta
     1081 caggcgacat cgtattttgc tgcatcagct aaaatcttca atttttcatt taaagataaa
     1141 tcttttgatg agatttccat atcaaacagt tcctttcgaa tatatgttct agtttaacat
     1201 aaataaacgt aatttacaaa ttgtattttg atctgatcat cacatatatt tgaaaaaaac
     1261 caccacatta gtgaccatta agatcataaa tgtgatggct tttttctagc caagaatctt
     1321 cttattcgat acagctgcat cctgataaga atatgacttg gttgtcagag attctaaaat
     1381 ctgctcagga tgaacaatgg catcttctaa agatgtgaca taaagctttt tattaccgaa
     1441 gcgggaagct tcgtcgatcg caacatcagc agcgatcaca atataatcag cttcatcgat
     1501 ctgttcaggg gaaaactgat tttgaattcc catagctccc tgtgtttcca cagaaaggtc
     1561 aacgccaagt ttcttacctg cctgttctag tttctctgcc gcaacatagg tttgaaccat
     1621 tccagtcggg cattttgtta cacacaaaat tttcatgaaa tcctccaatc gtatgttact
     1681 ccttcttgat agtcagtata gcatgagata gtagaaaagg aaaagcagaa tgatagaaaa
     1741 aatagttttt gttaaaagtg catagaaata aagtgaaaat attgttaata tcgaccataa
     1801 acaacaactt gtaaagagga ccgtacaagt tttaaaataa gaaccataag gaaggaggat
     1861 gtcaagcatg ggaaagaaca aaggagaaat gaaatatcac acattaaagg aatcactgaa
     1921 gaatgatatt ttatctggtg tctatcaacc gggagataag atgccgtcag agaatcaact
     1981 agtacaggaa tatgatctta gccgccatac cgtacgtaag gcgctgtcaa ttctggaaga
     2041 agaaggttat atcattgcag ttcatggaaa gggaacgttt tgtacagaac gggcacttca
     2101 taaaaaggaa tcaaagaaca ttgcagtggt tacaacctat atttctgatt atatattccc
     2161 tagattgatc caggggatca acagtgtcct ttccaaagat ggatacagca ttatttttaa
     2221 gacaacagaa aatagcagac gaggagagac agcatgtctt gaagatattc ttacaaaaga
     2281 tattgatggc ttgatcattg agccaagtaa aagtgagatt gtatgcagac atccaaaact
     2341 ttatgagaat cttgataaat atgaaattcc ctacattttt attcacggag tctatgagga
     2401 gatgaaagac aaaccccaca ttcttatgga cgactgtaaa ggaggttatc tgcttacaaa
     2461 atatctgatc gatatgggac atcaacatat cgtaggagtt tttaaagcag atgatcatca
     2521 aggaagagaa cgacataaag gttatgtgat ggcacttcag gaagcaggat atcaatatga
     2581 tccagatatg gttgtctggt ttcatacaga agatcgtcag atgaaaccgg taacatcggt
     2641 tgttcagatg ataaaggatc aaagaaagat ggatgcagtt gtctgttaca atgaccagat
     2701 cgcattttca atcattggag gattgcatca gattggtgtt tcaattccag aagacttgtc
     2761 gatcacagga tatgataatt caatgcttgc acatcagcat ccactttctt taacgactat
     2821 tgctcatcca caggaaaaac ttggcgagat ggcaggacag ttgttgctag aaaagataca
     2881 aggtggatca gataaagaca tgaaaacaga gtatttgatc gaaccgaaat tggtgatcgg
     2941 aaactcatgt aaaaatagga atcaagaata gatataaaac tgggaggcaa aaaatggaac
     3001 ttaaaaaaaa ttacaaattt tggtttgcaa caggatcaca ggatttatat ggagatgaat
     3061 gtttaagcca tgtggcaaaa catgcacaga tcattgtgca gaatttcaat gaaagtggta
     3121 tcttaccatt tgaagttgta tggaaaccga cattaattga tagtacttcg atcagaagaa
     3181 catttgaaga agcaaatgca gatgaagaat gtgccggtgt catcacatgg atgcacacat
     3241 tctccccagc aaaatcatgg atcgcaggat tacaggcatt taagaaacca ttattacatc
     3301 ttcacacaca gtttaatgaa gaaatcccat atgacacgat tgatatggat tttatgaatg
     3361 aaaaccagtc agcacatggt gacagggaat ttggacatat cgtaacaaga atgggaatcc
     3421 caagagaagt tgttgttgga tattatgaat caaaagaagt gaaagaaaag atcgcatctt
     3481 ggatgagaag tgcaatcgga atggtggaaa gtaaaaatat ccgtgtggta aggattgcgg
     3541 ataatatgag aaatgttgcg gttacagaag gtgataaagt cgaagcacag attaaatttg
     3601 gatgggagat cgatgcatat ccagtcaatg aagttgtaga ttatgtcaat gcagtcagca
     3661 aaggtgatat tgatgcactg acacagttat attatgaaaa atacaatctt ttattagagg
     3721 gcagagatcc gatcgaattt aagaaacatg tagaggtaca ggcaggaatc gagatcggac
     3781 tggaaaaatt cttaaaagac catgattatc aggcagttgt tacacacttt ggcgatcttg
     3841 gtggattaaa acagcttcca ggacttgcga ttcagagatt aatggaaaaa ggttatggat
     3901 ttggtgcaga aggtgactgg aagacagcag caatggtacg actgatgaaa gttatgacag
     3961 caggaaagaa agatgccaaa ggaacatcat ttatggaaga ttatacatac aattttgttc
     4021 cgggaaaaga gggtatctta gaagctcata tgttggaagt atgcccaacg atcgcggatg
     4081 gtcctgtttc aatcaaagta aatccattat ctatgggaga tcgtgaagat cctgcaagac
     4141 ttgtatttac agcaaaagaa ggagaaggaa tagccacttc tttgatcgat cttggacatc
     4201 gtttccgtct gatcattaat aaagtggatt gtaaaaagac agaaaaacca atgccagagc
     4261 ttccagtagc gacggcattc tggacaccaa aaccagatct ttataaagga gcagaggcat
     4321 ggatcttagc tggtggagca catcatacag cattttctta tgatcttagt gtagatcaga
     4381 tggttgcatg ggcagaggct atgggaattg agagtgttgt cattgatgag aatacagata
     4441 tcagaatatt aaagaatgaa ttaagatgga atgaagtagt ttatcgttag aaaatggagg
     4501 tagtatcatg gaaacaaagc aaatgatcgc agatggaaaa acagcattgg gaatcgaatt
     4561 tggatcaaca aggatcaaag ttgtactgat cgacaaagat catcatccga ttgcatccgg
     4621 aagccatgac tgggaaaatc gtctagagaa taatatatgg acttatacat tagaagacat
     4681 ctggagtgga ctacaagaca gttaccaaaa cttggtaaac gacgtgaaag aaaaatatga
     4741 tattacacta actaaggttg gaagtatcgg atttagtgcg atgatgcatg gatatatggc
     4801 atttgataaa gatggagaat tgttagtgcc attccgaaca tggagaaata caatgacaga
     4861 agaagcttct gagaaattaa cggaagcatt ttcttatcac attcctcaaa gatggagtat
     4921 cgcacatctg tatcaggcaa ttttaaaaga agaagaacat gtaaaagata ttgattatct
     4981 gacaacactg gcaggatatg tccattggaa attaacaggt gaaaaggtgc tcggtgttgg
     5041 agaagcgtct ggaatgttcc caatcgatat cgaaacaaaa gattataacc agacgatgat
     5101 caagacattt gacgacctga tcaaagataa aaaatttgga tggaaattag aaaatatctt
     5161 accaaaagtt ttaatagctg gagataaagc gggtatctta agcgaagaag gagcaaaatt
     5221 attagatcca tctggtaatc tggaagctgg aattccttta tgtcctccag aaggagatgc
     5281 aggaactgga atggttgcga caaacagcgt gacaagaaaa acaggaaatg tatctgcagg
     5341 aacaagtgtt tttgcaatga tcgttttaga gaaagaatta aagaaagttt atgatgagat
     5401 cgatcttgtc acaactccat caggtgatct tgttg
//
LOCUS       DAOMUE010000122         5368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4208_length_5368_cov_2.430642, whole genome shotgun sequence.
ACCESSION   DAOMUE010000122 DAOMUE010000000
VERSION     DAOMUE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4208_length_5368_cov_2.430642"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="PLC71_06500"
     CDS             complement(<1..134)
                     /locus_tag="PLC71_06500"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HRM02325.1"
                     /translation="MAKPNKAVVVFSGGQDSTTCLFWAKKKYDEVIALSFNYGQRHSK
                     "
     gene            complement(153..740)
                     /gene="folE"
                     /locus_tag="PLC71_06505"
     CDS             complement(153..740)
                     /gene="folE"
                     /locus_tag="PLC71_06505"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRM02326.1"
                     /translation="MAIDKEAIKEHIRGILVALGDDPNREGLKETPDRVAKMYEEVFE
                     GMNYSNHEIAQMFSKTFEDDLSVKDHKDMVIVKDIDIFSYCEHHMALMYDMKVSVAYL
                     PKDKVLGLSKIARICDMVGKRLQLQERIGSDIAEIIMEVSGSEDVAVYIEGCHSCMSA
                     RGIKKHNAKTVTTTLRGAFDTDSTLKWHFMMQAGK"
     gene            complement(752..1420)
                     /gene="queE"
                     /locus_tag="PLC71_06510"
     CDS             complement(752..1420)
                     /gene="queE"
                     /locus_tag="PLC71_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRM02327.1"
                     /translation="METFKVVETFVSINGEGKKAGRLAMFIRLKGCNLNCSYCDTTWA
                     NKRDARCELLTAPQIVERIKEAGVELVTLTGGEPLLDENVSELIGSILMMPKVEIEIE
                     TNGSVPIRYYKERDNRLTMTMDYKLPSSNMEENMCLENMEYLKPWDVVKFVIGSREDL
                     NRAKEIIERFRLCEKAIVYFSPVFGKIEPEEIVEFMKENKLNKVRFQIQIHKVVWDPD
                     KTGV"
     assembly_gap    1450..1459
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1460..1846)
                     /gene="queD"
                     /locus_tag="PLC71_06515"
     CDS             complement(<1460..1846)
                     /gene="queD"
                     /locus_tag="PLC71_06515"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HRM02328.1"
                     /translation="MYQLTTHASFDSAHFLSGYEGKCSNIHGHRWKLEVTVQSETLEQ
                     TGQIRGMVVDFGQLKDDIKKLADEFDHSLIIEDGTLKEKTVEALKEENFKMIFVDFRP
                     TAESFAKYFYDRVKEIGYDVKLAKVYE"
     gene            complement(2121..4082)
                     /locus_tag="PLC71_06520"
     CDS             complement(2121..4082)
                     /locus_tag="PLC71_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HRM02329.1"
                     /translation="MRITELLQDATIDLGVKVNSKEEAIDHLVDLMVKGGNITDREEY
                     KKGILAREANGTTGIGGGIAIPHSKNKAVTKAGLASMTVPDGVDYEAMDGEPSDVFFM
                     IAAPAEGSDVHLEALSRLSVILMDPVFKDSLLKATSKEEYLALIDKKETEKFPEEAAK
                     EEEKPAVQEEKADDGTIRVLAVTACPTGIAHTFMAAESLENKAKEMGITLKAETNGSG
                     GAKNVLTKEEIENCDGIIVAADKNVEMARFDGKQVLQTKVADGINKPEELIQTILDKK
                     APTYHHSGAAAQEDSAEGESVGRQIYKHLMNGVSHMLPFVIGGGILIALAFLVDNQAI
                     NPANFGKNTPLAALLKTIGEQAFGFMLPILAGFIAMSIADRPGLAIGFVGGALANGGY
                     TFANIMAYDSAKAVSSGFIGALFAGFVGGYIMVLLRKLFDKLPSALEGLKPILLFPVC
                     GILIMGVVMIAVNPVVGAINTGLNNFLSSMSGTSSILLGAVLGAMMSIDMGGPFNKAA
                     YVFGTAQLTVANAGPEQYAIMAAVMAGGMVPPLAIALCTTFFKNRFTESERKSGVVNY
                     IMGLSFITEGAIPFAAGDPIHILPPCIVGSAVAGALSMAFKCGLPAPHGGIFVIGVIT
                     NPVQYLISVVVGAVIGMIVMSFTKKPLNK"
     gene            complement(4265..5323)
                     /gene="rsmH"
                     /locus_tag="PLC71_06525"
     CDS             complement(4265..5323)
                     /gene="rsmH"
                     /locus_tag="PLC71_06525"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRM02330.1"
                     /translation="MEQQQPEKKHKRRVRYKGTHPRRFEEKYKELNPEKYGDTIQKVI
                     SKGSTPAGMHISICVKEILEFFDIQPGQKGLDATLGYGGHTRKMLEKLQGKGHIYALD
                     VDPIESVKTEKRLHDAGFGEDILTIKHINFANIDQVAEEVGPFDFILADLGVSSMQID
                     NPERGFSYKFEGPLDLRLNPEKGISAAERLEDITKAEFEGMLIENADEPYAKQIAKET
                     VATMKRGKKIKTTTDLKDVIERALNFLPEKERKEAVKKSCQRTFQALRIDVNSEFEVL
                     EELLEKLPDALAPGGKVAILTFHSGEDRLVKHAFKYGKKVGIYSDVAKDVIRPSKEEC
                     TQNGRAKSTKMRWAIKVK"
BASE COUNT         1401 a         1223 c          797 g         1937 t
ORIGIN      
        1 tcctttgaat gtctctgtcc atagttaaat gaaagcgcga tcacttcatc atatttcttc
       61 tttgcccaga aaaggcatgt tgtgctgtct tgtccaccgc tgaatacaac aacggccttg
      121 tttggttttg ccattttgct tctctccttt tcttattttc ctgcctgcat catgaaatgc
      181 cattttaatg tggaatctgt atcaaatgct cctcgaagtg ttgttgtcac tgtcttggca
      241 ttgtgttttt tgattcctct tgcactcatg cagctatgac atccttcaat ataaacagct
      301 acatcttcac ttcctgaaac ttccatgatg atctctgcga tatcacttcc gatcctttcc
      361 tgaagctgta aacgttttcc aaccatatca cagattcttg cgatcttact aagtcctaat
      421 accttgtctt ttggcaaata tgccacagag actttcatat catacattaa tgccatatga
      481 tgctcacaat aactaaagat atcaatatct tttacgatca ccatatcttt atgatctttg
      541 acacttagat catcttcaaa tgtctttgaa aacatctgtg cgatctcatg attgctgtaa
      601 ttcattcctt caaagacttc ttcatacatt ttcgctacac gatctggtgt ttcttttaac
      661 ccttcacggt ttggatcatc tccaagtgca acaagaatac ctcttatatg ttccttgatc
      721 gcttctttat cgattgccat agtatttcct cctatacgcc tgtcttgtca gggtcccata
      781 caactttatg gatctgaatc tggaacctga ctttgttaag cttgttttct ttcataaatt
      841 caacgatttc ttctggttcg attttaccaa atacaggact gaagtataca attgcttttt
      901 cacaaagtct gaatcgttca atgatttctt tcgccctgtt taaatcctcc ctgcttccaa
      961 taacaaattt aacaacatcc catggtttta aatattccat attttcaagg cacatattct
     1021 cttccatatt actggatgga agtttgtaat ccattgtcat tgttaatctg ttgtctcttt
     1081 ctttataata tctgatcggt acacttccat ttgtctcaat ctcaatctca acttttggca
     1141 tcatcaagat acttccgatc aattctgata cattttcgtc aagaagaggc tctccacctg
     1201 ttaatgtaac aagttcgacc cctgcttctt tgatcctttc tacaatctgc ggtgccgtca
     1261 ataattcaca tcttgcatct cttttattgg cccatgttgt gtcgcagtaa gaacagttta
     1321 agttacatcc tttcaatcgg ataaacatag caagacgacc agctttcttt ccttctccgt
     1381 tgatacttac aaaggtttcg actactttaa atgtttccat ctcatttact ccccatatgc
     1441 tgccatattn nnnnnnnnnc tcataaactt tcgctaactt gacatcatat ccgatttctt
     1501 tcactctatc ataaaaatat tttgcaaaac tctctgcagt tggacgaaaa tctacgaaga
     1561 tcattttgaa attttcctct ttcaatgctt ctactgtctt ctcttttaat gttccatctt
     1621 ctatgatcag actatgatca aactcatctg ctaatttttt aatatcatct tttaactgtc
     1681 caaaatcgac aaccattcct cgaatctgcc ctgtctgttc taatgtttca ctttgtacgg
     1741 taacttctaa tttccagcgg tgaccgtgga tattgctgca ttttccttca taacccgata
     1801 aaaaatgagc tgaatcaaag gatgcatgtg ttgttaactg atacattttc ttctttccct
     1861 tctacatata ttttcaatta aaaaaggata caagcacgcg tacctgtatc ctgaaaatct
     1921 tcttctcagt cctagttttg tttatagaca ggatggtacg aatgaactgt ctttgatttc
     1981 taaaatctta tctagtatat cattgtttta tcaaaatagc aaacagagat tgcaaaattt
     2041 cacaatctct gtttacttat tataaatatt gcttatgaaa atatgaggaa ttaatttatt
     2101 gtaaatggtt atttacaatc ttatttattt aatggtttct ttgtaaatga cataacgatc
     2161 attccaataa ctgcaccaac tactacagaa attaagtatt gtactggatt cgtgatgact
     2221 ccaataacaa agattccacc gtgtggtgct ggaagtccac atttaaatgc catggataac
     2281 gcacctgcta ctgcagatcc tacgatacat ggtggcagga tatggattgg atctcctgct
     2341 gcaaacggga tagctccttc tgtaatgaat gataatccca tgatgtaatt tacaacacca
     2401 gattttcttt cactctctgt aaaacgattc ttgaagaatg ttgtacaaag tgcgatcgca
     2461 agtggaggta ccattcctcc ggccataaca gctgccatga tcgcatactg ttctggtcct
     2521 gcattggcta ctgttaactg tgctgtacca aatacatacg ctgctttatt aaatggacca
     2581 cccatatcaa tggacatcat cgctccaaga actgctccta aaaggataga acttgttcca
     2641 ctcatagatg ataagaagtt attcagtcct gtattgatcg ctccaacaac cggattaact
     2701 gcgatcatca caacacccat aattaaaata ccacaaactg ggaatagtaa aattggtttt
     2761 aacccttcta atgcacttgg taatttatca aacagttttc ttaataaaac catgatatat
     2821 ccaccaacaa aacctgcaaa taatgctccg ataaatccag aggaaacggc tttggcagaa
     2881 tcatatgcca tgatattagc aaatgtataa ccaccatttg ctaatgctcc accaacgaaa
     2941 cctatcgcta atcctggtcg gtctgcaata ctcattgcaa taaatccagc taagattgga
     3001 agcataaatc caaatgcctg ttcaccgatt gtctttagta aagctgcaag tggtgtattc
     3061 ttaccgaagt ttgccgggtt gatcgcctga ttatctacta agaatgcaag agcgatcaag
     3121 atacctccac cgattacaaa tggcagcata tgagatacac cattcatcag atgtttataa
     3181 atctgacgtc caacgctttc accttctgca ctatcttctt gtgctgctgc tcctgaatga
     3241 tgatatgttg gcgctttctt atctaaaatt gtctggatca gttcttctgg tttattaatt
     3301 ccgtccgcta cttttgtctg aagaacctgt tttccatcaa aacgtgccat ctcaacgttc
     3361 ttatctgctg ctacgatgat tccatcacaa ttttcaatct cttcttttgt taagacattc
     3421 tttgctccac ctgaaccatt tgtctcagct tttaatgtaa ttcccatctc ttttgcttta
     3481 ttctcaagag attctgctgc catgaatgta tgagcgattc ctgttgggca tgctgtaact
     3541 gcaagaactc tgatcgttcc atcgtctgct ttctcttctt gtacagcagg tttctcttct
     3601 tccttcgccg cctcttctgg gaatttttct gtttctttct tgtcaatcaa tgcaagatat
     3661 tcttccttgc ttgttgcctt taataaactg tccttaaata ctggatccat taagatcaca
     3721 gataatcttg acaatgcctc taaatgcacg tcgcttcctt ctgctggtgc tgcgatcata
     3781 aaaaatacat cagatggttc tccatccatt gcttcataat cgactccgtc tggtactgtc
     3841 atagaagcca aaccagcctt agttactgct ttgttcttag aatgaggaat tgcaatccct
     3901 cctccgatac cagttgttcc attagcttct cttgctaaaa tgcccttctt gtattcttcg
     3961 cgatctgtga tgtttccgcc cttgaccatt aaatcaacca gatgatcaat tgcttcttct
     4021 ttgctgttaa ccttcacacc aaggtcaatt gttgcatcct gcaacagctc tgtaattctc
     4081 atacaggtta tcctcctttt ccaaaataaa aaacgtcaaa atcaatcatt cgtttctttt
     4141 ttttcttcat tatagcacag tttcagtcaa agtcaatcat tttaaaccat tttctttcat
     4201 gtttttgaaa aaaggaaata ttaaaaagca tacctctttt taatatttcc cttcctttta
     4261 atttttattt cactttgatc gcccagcgca tctttgttga ttttgctctt ccgttctggg
     4321 tacattcctc ttttgatgga cgaataacat ctttggctac atcactgtag attcctactt
     4381 tcttcccata cttaaacgca tgttttacaa gtcgatcttc tccagagtgg aatgtaagga
     4441 tcgcaacctt tccacctggt gctaatgcgt ctggaagttt ctccaataat tcttctaata
     4501 cttcaaactc gctgtttaca tcaatacgca aagcctgaaa tgttctctgg caggattttt
     4561 taactgcttc ttttcgctct ttttccggca aaaagtttaa tgctctttca atgacatctt
     4621 taagatccgt tgttgtcttg atcttctttc ctcgtttcat cgttgcaacg gtttcttttg
     4681 cgatctgttt tgcatatggc tcatctgcat tttcaatgag catcccttca aattcagcct
     4741 tcgtgatatc ttctaaacgc tctgccgctg aaattccttt ttctggattc agtcttaaat
     4801 ctaatggtcc ttcaaattta taagaaaatc ctctttccgg attatcaatc tgcatggagg
     4861 atacgccaag gtcggctaaa atgaaatcga atggtccaac ttcttcggct acctgatcaa
     4921 tattagcaaa attaatatgc ttgatcgtta agatatcttc tccaaaccct gcatcatgca
     4981 gacgtttctc tgtctttaca gattcgatcg gatctacatc taatgcgtag atatgacctt
     5041 tgccctgtaa cttttctaac atctttcttg tatgtccgcc atatcctaag gttgcatcca
     5101 atcctttttg tcccggctgg atgtcaaaaa attcaagaat ttcttttaca cagatggaaa
     5161 tgtgcattcc tgccggtgta cttcctttac taataacttt ttgaatcgta tctccgtatt
     5221 tttctggatt taattctttg tatttctctt cgaatcttct tggatgagtc cctttatatc
     5281 tgactcttcg tttgtgtttc ttttctggct gctgctgttc cattgttttc tacctttctt
     5341 tgttataatc aggttaatct aagattag
//
LOCUS       DAOMUE010000123         5352 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4221_length_5352_cov_2.855012, whole genome shotgun sequence.
ACCESSION   DAOMUE010000123 DAOMUE010000000
VERSION     DAOMUE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4221_length_5352_cov_2.855012"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /gene="leuB"
                     /locus_tag="PLC71_06530"
     CDS             <1..733
                     /gene="leuB"
                     /locus_tag="PLC71_06530"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRM02331.1"
                     /translation="KKACPLRDEVIGDGFDMVIMRELTGGLYFGERHTEEIDGVRTAV
                     DTLSYNENEIRRIAIKAFDIARKRKKHVISVDKANVLDSSRLWRAVVEEVAKDYEDVT
                     YEHMLVDNCAMQLVNNPRQFDVILTENMFGDILSDEASMITGSIGMLSSASLAEGKFG
                     LYEPSHGSAPDIAGQDIANPIATILSAAMMLRYSFDLDKEADEIEKAVKAVLADGYRT
                     VDIMSDGMKKVGCKEMGQLIIDQIK"
     gene            790..2454
                     /gene="ilvD"
                     /locus_tag="PLC71_06535"
     CDS             790..2454
                     /gene="ilvD"
                     /locus_tag="PLC71_06535"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRM02332.1"
                     /translation="MSNTFTEGEAFAPQRSLFNALGFTKEEMRKPLVGIVSSYNEIVP
                     GHMNIDKIVDAVKLGVAMAGGTPVVFPAIAVCDGIAMGHVGMKYSLVTRDLIADSTEC
                     MALAHQFDALVMVPNCDKNVPGLLMAAARVNVPTVFVSGGPMLAGRVKGCKTSLSSMF
                     EAVGSYAAGKITKEELDEFENKTCPTCGSCSGMYTANSMNCLTEVLGMGLQGNGTIPA
                     VYSERIRLAKHAGMKVMELLEKNIRPRDILTEKAFENALTVDMALGCSTNSMLHLPAI
                     AHEAGVDLNLEKANEISKRTPNLCHLAPAGHTYIEELNEAGGVYAVMNELNKKNLLHT
                     DLITATGKTVAENIEGCVNKNPEVIRPIDDPYSQTGGIAVLKGNLAPDSGVVKRSAVA
                     PEMLKMEGPARVFDCEEDAIKAIKGGDIKPGDVVVIRYEGPKGGPGMREMLNPTSAIM
                     GMGLGSSVALITDGRFSGASRGACIGHVSPEAAVGGPIALVEEGDIIAIDIDANTLNV
                     KVSDEELAKRKENWKPRTPKVTTGYLARYASLVTSGNRGAVLEIKQ"
     gene            2480..4168
                     /gene="ilvB"
                     /locus_tag="PLC71_06540"
     CDS             2480..4168
                     /gene="ilvB"
                     /locus_tag="PLC71_06540"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRM02333.1"
                     /translation="MKQLTGAQIVLECLKEQGVDTVFGYPGGAILNIYDELYKQKKDF
                     THILTSHEQGASHAADGYARSTGKVGVCFATSGPGATNIVTGLATANMDSVPVVAITC
                     NVGVSLLGKDSFQEVDISGVTIPITKHNFIVKNIEELADTIRRAFRIAKTGRPGPVLI
                     DIPKDVTAATTLYEKYKPAKIIRSSEYIKEVDIQNAISMIREAKRPYILVGGGAVISD
                     AADELKEFAEKVDAPVCDTLMGKGAFNGTDERYSGMLGMHGTKASNLGVSHCDLLIAV
                     GTRFSDRVLGNPKTFAKNAKILQIDVDPVEINKNICVSASIIGDVKAVLTVINRRLEA
                     QEHKEWLDTVKEYKEKYPLTYDKGRLTCPEIMEAIYDITKGEAVITTEVGQHQMWAAQ
                     FYKYDKPRKFLTSGGLGTMGYGLGASLGAKVGNPDKIVVNIAGDGCFRMNLNELATAS
                     RYNIPVIEVIINNSVLGMVRQWQDLFYEERYSYTTLQDKVDFVKVAEGLGVQAKKITD
                     IEQFKDAFKEALNAGEPVVLDCHIDLDDKVWPMVNPGGSIEDAFDKSDLEAQAR"
     gene            4205..5287
                     /gene="serC"
                     /locus_tag="PLC71_06545"
     CDS             4205..5287
                     /gene="serC"
                     /locus_tag="PLC71_06545"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRM02334.1"
                     /translation="MARVYNFSAGPAVLPEEVLKEAAEEMLDYRGCGMSVMEMSHRSK
                     MFDDIIKTAEADLRELMNIPDNYKVLFLQGGASQQFSAIPMNLMKNKVADFIITGQWA
                     KKAYQEASRYGKANILASSEDKTYTYIPDCSDLPVSEDADYVYVCYNNTIYGTKYQQI
                     PNTKGKILVADMSSCILSEPVNVEDFGVIYFGVQKNVGPAGVVVCIVREDLITDDVLE
                     GTPTMLKWKTQADADSLYNTPPCYGIYICGKVFKWLKDQGGLTAMKKRNEEKAKILYD
                     FLDQSEMFKGTVVKEDRSLMNVPFVTGDKDLDAKFVAEAKEAGFENLKGHRTVGGMRA
                     SIYNAMPKEGVEKLVAFMKKFEEENK"
BASE COUNT         1866 a          853 c         1253 g         1380 t
ORIGIN      
        1 aaagaaagca tgtccacttc gtgatgaagt gatcggtgat ggatttgata tggtcatcat
       61 gagagaatta acaggtggtc tttactttgg agaaagacat acagaagaaa ttgatggtgt
      121 aagaacagcc gttgatacat tatcttacaa tgaaaatgaa attagaagaa ttgcgatcaa
      181 agcatttgac attgcaagaa aacgtaaaaa acatgtgatc agtgttgata aggcaaatgt
      241 acttgattct tccagattat ggagagcagt tgtagaagaa gtagccaagg actacgaaga
      301 tgttacatat gaacatatgt tagttgataa ctgtgcaatg cagttagtca ataatccacg
      361 tcagtttgat gtgatcttaa cagagaatat gtttggagat attttatcag atgaagcaag
      421 tatgatcaca ggttctattg gaatgttatc atccgcaagt cttgcagaag gtaagtttgg
      481 attatatgag ccaagccacg gatcagctcc agatatcgca ggacaggata ttgcaaaccc
      541 aatcgcaaca attctttccg cagctatgat gcttcgttat tcatttgatt tagataaaga
      601 agcagatgag atcgaaaaag cagtcaaagc agttttagca gatggataca gaacagttga
      661 cattatgtca gatggaatga agaaagtcgg atgtaaagaa atgggacagc tgatcattga
      721 tcagatcaaa taagaattcg atgctcacca tagatagaat gagatatatt atactaggag
      781 gttagtttta tgagtaatac atttacagag ggagaagcat ttgcacctca aagatcgtta
      841 tttaacgcat taggtttcac aaaagaagag atgagaaagc cattggtcgg aatcgtaagt
      901 tcgtataatg agatcgtgcc agggcatatg aatattgaca agatcgtaga tgcagtaaaa
      961 cttggagttg ctatggcagg tggaacacca gttgtattcc cagcgatcgc agtatgtgat
     1021 ggaattgcca tgggacatgt tggaatgaaa tattcactgg tgacaagaga tctgattgct
     1081 gattctacag aatgtatggc attagcacat cagtttgatg ctttagttat ggttccaaac
     1141 tgcgataaga atgtaccagg actattaatg gccgctgcac gtgttaatgt acccacagtg
     1201 ttcgtcagtg gcggaccaat gcttgccgga cgtgtaaaag gatgtaaaac aagcttatca
     1261 agcatgtttg aggctgttgg atcttatgca gcaggtaaga tcacaaaaga agaattagat
     1321 gaatttgaaa acaaaacatg tcctacatgt ggatcatgtt ctggtatgta cacagcaaac
     1381 tctatgaact gtttaacaga agttcttggt atgggattac agggaaatgg aacaattcca
     1441 gctgtatatt ctgaaagaat ccgtcttgca aaacatgcag gtatgaaggt tatggaatta
     1501 ttagagaaga atatccgccc aagagatatc ttaacagaga aagcgtttga gaatgcttta
     1561 acagttgata tggcacttgg atgcagtaca aacagtatgc ttcatttacc agcaatcgca
     1621 catgaagcag gtgttgatct gaatcttgag aaggcaaatg agattagtaa gagaactcca
     1681 aacttatgtc atcttgcacc agcaggacat acatatattg aagagttaaa tgaagctggt
     1741 ggagtttatg ctgttatgaa tgaattaaat aagaagaatc ttcttcatac agatcttatc
     1801 actgcaacag gtaagacagt agcagagaac attgaaggat gcgtaaacaa gaatccagaa
     1861 gttattcgtc cgattgacga tccatacagc cagactggtg gaattgctgt attaaaagga
     1921 aatttagcac cagattcagg tgttgtaaaa cgttctgcag ttgctcctga gatgcttaag
     1981 atggaaggac cagccagagt ctttgattgt gaagaagatg cgatcaaggc gatcaaaggt
     2041 ggagatatta aaccagggga tgtcgtagtg atccgctatg aaggaccaaa aggtggacca
     2101 ggtatgagag agatgttaaa tcctacatca gcgatcatgg gtatgggact tggatcaagc
     2161 gtagccctga tcacagatgg aagattctcc ggagcttcca gaggagcttg tatcggacat
     2221 gtttctccag aagcagcagt tggaggaccg attgcattag tagaagaagg agatattatt
     2281 gcaattgata tcgatgccaa tacattaaat gtaaaagttt ctgacgaaga attagcaaag
     2341 agaaaagaaa actggaaacc tagaacacct aaggttacaa caggttatct tgcaagatat
     2401 gcatcgttag ttacatctgg aaaccgcgga gcagttttag aaatcaaaca gtaattaagt
     2461 aagaaaagag gatcaaatga tgaaacaatt gacaggtgct cagattgttt tagaatgttt
     2521 gaaagagcaa ggcgtggata ccgtttttgg gtatccaggc ggagcgatct taaacattta
     2581 tgatgaatta tataaacaga aaaaggattt tacacatatc ctgacatctc atgagcaggg
     2641 agcctctcat gcagcagatg gatatgcaag atctacagga aaagtcggag tgtgctttgc
     2701 aaccagtgga ccgggagcga caaatatcgt tacaggactt gcaacagcga atatggattc
     2761 agtgccagtt gttgcgatca catgtaacgt aggagtcagc cttcttggaa aggacagctt
     2821 ccaggaagtt gatatttccg gagtcacaat tccgatcaca aagcataact ttattgtaaa
     2881 gaatatagaa gaacttgcag acacgatccg aagagcgttt agaattgcaa agacaggaag
     2941 accagggcct gttttgatcg atattccaaa agatgtgaca gcagcaacga ccctatatga
     3001 aaaatacaaa ccagcgaaga tcatacgttc cagtgaatat atcaaagaag tagatatcca
     3061 gaatgcgatc agcatgatca gagaagcaaa acgaccatat atcttagttg gtggtggagc
     3121 tgtgatctct gatgcagcag atgaattaaa agaatttgca gagaaagtag atgcaccagt
     3181 atgcgatacc ctcatgggaa aaggagcatt taatggtacg gatgaacgct atagtggaat
     3241 gcttggaatg cacggaacaa aagcatcaaa tcttggagta tctcactgtg accttctgat
     3301 cgcagtcgga acaagattta gtgaccgagt tttaggaaat ccaaaaacat ttgcaaagaa
     3361 tgcaaagatt ttacagattg atgttgatcc agtcgagatc aacaagaata tttgtgtatc
     3421 agcttctatc atcggagatg taaaagcagt attaacagtg atcaatcgcc gtttagaagc
     3481 ccaggaacac aaagagtggc ttgatacggt caaagaatat aaagaaaaat atccattaac
     3541 ttatgataaa ggacgattaa catgtcctga gatcatggaa gcgatttatg atattacaaa
     3601 aggtgaggca gtcattacaa cggaagttgg acagcatcag atgtgggccg ctcagtttta
     3661 taaatatgat aaaccacgta aattcttgac atctggtggt cttggaacaa tgggatacgg
     3721 ccttggcgcc agccttggag ccaaagttgg taatccagat aagatcgtag taaacattgc
     3781 aggagatgga tgtttccgta tgaatttaaa tgagcttgcg acagcctcgc gatacaacat
     3841 tccagtgatc gaagttatta tcaataacag cgtgttagga atggtaagac agtggcaaga
     3901 tctgttttat gaagaaagat attcgtatac aacacttcag gacaaagtag actttgtgaa
     3961 agttgcagaa ggacttgggg tgcaagctaa gaaaatcacg gatatcgaac aattcaaaga
     4021 tgcattcaaa gaagccttaa atgcaggaga accagtagtt ctcgattgtc atattgactt
     4081 agatgacaaa gtatggccga tggttaatcc aggaggttct attgaagatg catttgataa
     4141 atcggatctt gaggcgcagg caaggtaatt tttaatttat tttcatataa aggagagaaa
     4201 agaaatggca agagtataca acttttcagc tggaccggca gtacttccgg aagaagtgtt
     4261 aaaagaagca gcagaggaaa tgcttgatta cagaggatgt ggaatgtccg ttatggagat
     4321 gagccacaga tccaagatgt ttgatgatat catcaaaact gcagaagcag acttaagaga
     4381 attaatgaac atccctgata attacaaagt attattcctt cagggtggag catcccagca
     4441 gttttcagcg atccctatga atctgatgaa gaacaaagtt gcagatttca tcatcacagg
     4501 acagtgggca aagaaagcat accaggaagc ttccagatat ggaaaagcaa acattttagc
     4561 ttcatcagaa gataaaacat atacatatat tccagattgt tctgaccttc ctgtatcaga
     4621 agatgcagat tatgtatatg tatgctataa caacacgatc tatggaacaa aatatcagca
     4681 gatcccaaat acaaaaggta aaatccttgt tgcagatatg tcttcctgca ttttatctga
     4741 gccagttaat gtagaagact ttggagtgat ctattttgga gtacagaaaa acgttggacc
     4801 agcaggagtt gtagtttgta tcgtacgtga agatctgatc acagatgatg tgttagaagg
     4861 aacaccaacc atgttaaaat ggaaaacaca ggcagatgca gattcactgt ataacacacc
     4921 tccatgctac ggaatctata tctgtggaaa agtcttcaaa tggttaaagg accagggtgg
     4981 acttactgca atgaagaaac gtaacgaaga aaaagcaaag atcttatatg atttcttaga
     5041 ccagagtgaa atgtttaaag gaacagttgt gaaagaagac cgctctttaa tgaacgttcc
     5101 atttgtaaca ggagacaaag atctggatgc caaattcgta gctgaagcaa aagaagcagg
     5161 atttgagaac ctaaaaggac atcgtacagt tggtggaatg cgtgcaagta tttataacgc
     5221 aatgccaaaa gaaggtgttg agaaattagt agcatttatg aagaagtttg aagaagagaa
     5281 caaataagga ggagccatgt atacagtaaa atgcttaaac ccgatcgcaa gtaaaggact
     5341 agatttattt ac
//
LOCUS       DAOMUE010000124         5320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4264_length_5320_cov_2.727445, whole genome shotgun sequence.
ACCESSION   DAOMUE010000124 DAOMUE010000000
VERSION     DAOMUE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4264_length_5320_cov_2.727445"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..525)
                     /gene="rpmB"
                     /locus_tag="PLC71_06550"
     CDS             complement(340..525)
                     /gene="rpmB"
                     /locus_tag="PLC71_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRM02335.1"
                     /translation="MAKCAICGKGAHFGNAVSHSHRRSNKMWKSNIKSVKVKVNGAAK
                     KMYVCTSCLRSGKVERA"
     assembly_gap    594..611
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            727..1083
                     /locus_tag="PLC71_06555"
     CDS             727..1083
                     /locus_tag="PLC71_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRM02336.1"
                     /translation="MKGHVNTKNGEISIENQVIAKYAGICALGCFGIVGMAMVNMKDG
                     IAKLLTRDNIRKGVFVSVKNNKITIDFHIIVSYGVSISTVASNLMESVKYRVEEFTSL
                     EVERINIYVEGVKDIG"
     gene            1090..2763
                     /locus_tag="PLC71_06560"
     CDS             1090..2763
                     /locus_tag="PLC71_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRM02337.1"
                     /translation="MEAKIKMKQIDAALLQKMLIGGAKRLEANKEYINELNVFPVPDG
                     DTGTNMTMTALAAAKEVGNAAEVTVKEVTRGLSSGSLRGARGNSGVILSQLFRGFYKG
                     VKDQDVLTTETVAVGFKKAVETAYKAVMKPKEGTILTVAKVTAEKAVYCARNTEDFEE
                     FAQVVIKEANEILQKTPEMLPVLKEAGVVDSGGQGLVEFLQGAVDALMGKEVDLSSVE
                     KPAVKPAATASEAPLEEKDIKFGYCTEFIVMTKEEISMEEEQKFKDFLMGIGDSIVVV
                     ADEDIIKVHVHTNHPGKAIEKGLTYGELTNMKIDNMREEHRERLNLTQAEAQAEESKP
                     EEPRKDFGFVAVSIGQGMNDIFRELGVDYLIEGGQTMNPSTEDMLNAIEQVNAETVFI
                     LPNNKNIILAATQAQSLVEDKNVVIIPTTTVPQGISAIIGFDPEADAEENEDNMKDII
                     ECVKTGEVTYAVRDTSINGITIKVDDIMGIDDDGIKKVGQDVEKVTLELLEEMVDEDS
                     ELISIYYGEDTTKEQAEALLEKVEETYGDCDVQLEYGGQPIYYFLLSVE"
     gene            2847..4877
                     /gene="recG"
                     /locus_tag="PLC71_06565"
     CDS             2847..4877
                     /gene="recG"
                     /locus_tag="PLC71_06565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRM02338.1"
                     /translation="MDGTTNIIELKGIGEKSATAFGRLGIRDVDSLITMYPKYYLTYE
                     DPKDVNEIEIGQRVSIFVRINSEVHIQYAKRMKIVTCTAKDHTGTIMLVWYNMPYLKK
                     QLHQGRDYIFTGTPIYKNGRITMEHPEIFTQEDYEAKQETLQPVYPLTSGLTNKLVSK
                     AVAQTKDYIFHIPEYLPQNILDQYQPMEYHQAIWNIHFPESKEQLIKAKKRLIFDEFF
                     IFIAAMHMITSGEDLKEEGYKIGVCKEAKELVKNLPYELTTAQKRALNEMAKDMASGK
                     VMNRLVQGDVGSGKTILAVILLLMCAKAGYQGVLMAPTEVLAAQHYESFTELLESYDI
                     KIALLTGSTKAKEKRETYQKIKDGEVDIVIGTHAVIQDKVEYNKLALVITDEQHRFGV
                     RQRESLSLKGEKPHVLVMSATPIPRTLAIILYGDLDVSIIDELPAERLPIKNCVVNTS
                     YRPTAYRFIEKQVSQGRQVYIICPMVEESETMEGENVIQYAQMLRSQFAPSVRISYLH
                     GKMKAADKQKVMDDFSEGKIDVLVSTTVVEVGVNVPNATVMMVENAERFGLAQLHQLR
                     GRVGRGGYQSYCIFMTSSKKKETMQRLEVLNKSNDGFYIANEDLKLRGPGDFFGVRQS
                     GMMDFVLADIYTNADIMKQAADAVKQLEESGFDFSNLKNSRLEKQIGLARNI"
     gene            4893..5165
                     /locus_tag="PLC71_06570"
     CDS             4893..5165
                     /locus_tag="PLC71_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRM02339.1"
                     /translation="MVRFYLRFTGRVQGVGFRFFVAMNAERCHLTGWVKNMSDGSVTA
                     EVQGTRVEIEKFLNLIQTGNRFIRVDEIEKEERPYEENEKRFHVRY"
BASE COUNT         1853 a          797 c         1227 g         1425 t
ORIGIN      
        1 ggtgtgtatg ttaaacatta ttagttttca atttttgtag tggtagacag atgtttataa
       61 tattccacca ttaatttccg tcattcaaac cagaattttg gaatacatat gtacaaaaag
      121 gatcttcaaa tatgaaacga gtctgggcca tcgctttttt ctttttctca ttgggcttat
      181 ttacaggatt ctggatcact cacgatccga tgaaaatagt tatctgtatc ggatgtgata
      241 tcacagctta ctgtctattc tttaaataac gcttgaagaa taaaaaaaga gttggaataa
      301 attccaactc ttagtggttt cttatatgat caatcaagac taagcacgtt caaccttacc
      361 agatcttaag caagaagtac aaacgtacat tttcttagct gctccgttaa ctttaacttt
      421 aacagatttg atgttagatt tccacatttt atttgatctt ctatgtgaat ggctcactgc
      481 gtttccaaaa tgagcacctt tgccacaaat agcacatttt gccatgttag cacctcctta
      541 ttccaatctc tattcctgtt tcattccagg ctcgacaact atttcattct aacnnnnnnn
      601 nnnnnnnnnn nataaaaatc aagaataaat ttgcttttat tttaaatcat tttttgaaac
      661 actagactta ggtggtttca taatgtataa tcgaaatata tgaattctcg tttggaggta
      721 taatttatga aaggacatgt gaatacaaag aatggagaga tctccattga aaatcaggta
      781 attgcgaaat atgccggaat ctgtgctctt ggatgttttg gtatcgtagg gatggcaatg
      841 gtgaatatga aagatgggat cgccaagctg ttaacaagag ataatatcag aaaaggtgta
      901 tttgtttctg taaagaataa taagatcacg atagacttcc atattattgt atcttatgga
      961 gtttctattt ctacagtggc aagtaacctt atggaatctg taaagtatcg tgtagaagaa
     1021 tttacaagcc ttgaagtaga acggatcaat atttacgtag agggcgtaaa ggatatagga
     1081 taatagataa tggaggcaaa gattaagatg aaacagatag atgcagcttt gctgcagaag
     1141 atgttgattg gcggagccaa gagattagag gcgaataaag aatatattaa tgaactgaat
     1201 gtattccctg tgccagacgg tgatacagga acgaatatga caatgacagc tcttgcagct
     1261 gctaaagaag ttgggaatgc agcagaagtg acagtgaaag aagtaacaag aggattatca
     1321 agcggttctt taagaggagc cagaggtaac tcaggtgtta ttctatccca gttattcagg
     1381 ggattttata aaggtgtcaa agatcaggac gtattaacaa cagaaactgt tgcagtagga
     1441 tttaagaagg cagtagagac agcttataaa gcagttatga aaccaaagga aggaacaatc
     1501 cttacggtag cgaaagtaac agcagagaag gcagtatatt gtgcaaggaa tacagaagat
     1561 tttgaagaat ttgctcaggt agtgatcaag gaagcaaacg agattcttca gaagacacca
     1621 gagatgttac cagttctgaa agaagcagga gtggttgatt caggtggaca gggacttgtg
     1681 gaattcctac agggtgctgt tgatgcttta atgggtaaag aagttgattt aagcagtgtt
     1741 gagaagccag cagttaaacc agcagcaaca gcaagtgaag caccattaga agagaaagat
     1801 atcaaatttg gttattgtac agaatttatc gttatgacga aagaagagat ttccatggaa
     1861 gaagaacaga agtttaaaga tttcttaatg ggaatcggag attctatcgt ggttgttgca
     1921 gatgaagata ttattaaagt acatgttcat acaaaccatc ctggaaaagc gatcgagaaa
     1981 ggtctcacat atggagaact tacgaatatg aagatcgata atatgagaga agaacacaga
     2041 gaacgtttaa atctcacaca ggcagaagca caggcagaag aaagcaaacc agaagaacct
     2101 agaaaagact ttggatttgt ggcagtatcg atcggccagg gaatgaatga tattttccgt
     2161 gagttaggtg tagattacct gatcgaaggt gggcagacca tgaaccctag tactgaagat
     2221 atgttgaatg cgatcgagca ggtgaatgca gagactgtat ttattttgcc aaataacaag
     2281 aatatcattc ttgcagcgac acaggcacag tctttagtgg aagataagaa tgtagtgatc
     2341 attccgacaa caacagttcc acagggaatc tcagcgatca ttggatttga tccagaagca
     2401 gatgccgaag agaatgaaga caacatgaaa gacatcattg aatgcgtgaa gactggagaa
     2461 gttacttatg cagtcagaga tacatccatc aatggtatta cgatcaaggt cgatgatatc
     2521 atgggaatcg atgatgatgg gatcaagaaa gtcggacagg atgtcgagaa agtcacgtta
     2581 gaattactag aagagatggt agatgaagac agcgaactga tcagtattta ctatggagaa
     2641 gacacaacca aagaacaggc agaagcatta cttgagaaag tagaagaaac atatggtgac
     2701 tgtgatgtac agttagaata tggcggacag ccaatttatt atttcttatt atcagtagaa
     2761 taagagaaga tgggaaatcc cgcttgatgg cgggatttct ttgcttttcg tgagaatatg
     2821 ataaagaatt ttgaaggaga cagaagatgg atggcacaac aaatatcata gaattaaaag
     2881 gaattgggga aaagagtgcg acagcttttg gacgacttgg aatccgtgat gtagacagtc
     2941 tgatcacgat gtatccaaag tattatctta cctatgaaga tcccaaagat gtcaatgaga
     3001 tcgagattgg ccagagagta tctattttcg ttcgtatcaa ttcggaagtt catattcagt
     3061 atgcaaaaag aatgaagatt gttacatgca cagcgaaaga tcatacagga acgatcatgc
     3121 ttgtctggta caatatgcca tatttaaaga aacaattaca tcagggaaga gattatatat
     3181 ttacgggaac tccgatctat aaaaatggac ggatcacgat ggaacatcca gagatcttta
     3241 cacaggaaga ttatgaagct aaacaggaga cattgcagcc agtatatcca ttaacaagtg
     3301 gacttaccaa taaacttgtt tctaaggctg tggcacagac aaaagactat atctttcata
     3361 ttccagaata tcttccgcaa aatattttag atcaatatca gccaatggaa tatcatcagg
     3421 caatctggaa tattcatttt ccggaatcaa aagagcagtt gatcaaagca aagaaaaggt
     3481 tgatctttga tgaatttttt atttttatcg cagcgatgca tatgatcaca tcaggagaag
     3541 atctcaagga agaaggatac aagatcgggg tctgcaagga agcgaaagaa ctggtaaaga
     3601 atcttccata tgaattgact acagcgcaga aaagagcgtt aaatgagatg gcaaaagaca
     3661 tggcgtctgg taaggttatg aatcgtctgg tacagggtga tgtaggatct ggaaagacga
     3721 tccttgcggt tattttatta ttaatgtgtg ccaaagcagg ataccaaggg gttttgatgg
     3781 cgccaacgga agtgcttgca gctcagcatt atgaatcatt tacagaactt ttagaatctt
     3841 atgatataaa gatcgcattg ctgacaggtt ccacaaaggc aaaagaaaaa agagaaacct
     3901 atcaaaagat caaagatgga gaagtagata ttgtgatcgg aactcatgcg gtcattcagg
     3961 ataaggtgga atataacaag ttagcattag ttatcacgga tgaacaacat cgatttggag
     4021 taagacaaag agaaagttta tcgctaaaag gtgagaaacc acatgtactt gttatgagtg
     4081 cgacaccaat tccaagaaca ttggcgatca ttttatatgg tgatcttgat gtatcgatca
     4141 tcgatgaact gccggcagag cggttgccga tcaaaaactg cgtagtcaac acaagttacc
     4201 gcccgacggc atatcgtttc attgagaaac aggtcagtca gggaagacag gtatatatta
     4261 tctgtccgat ggtcgaagaa agtgagacga tggaaggaga aaatgtcatt cagtatgcgc
     4321 agatgttaag aagccaattt gcaccatcgg taaggatcag ttaccttcat ggaaagatga
     4381 aggcagcgga caagcagaaa gtcatggatg atttttcaga aggcaagatt gatgttttag
     4441 tatcaacaac ggttgttgaa gtcggggtga atgtgccaaa tgcaacggtt atgatggtgg
     4501 agaatgcaga acgttttgga cttgcacagc ttcatcagtt aagaggacgt gtcggacgtg
     4561 gcggctatca gtcctattgc atttttatga cgagttccaa gaagaaagaa acgatgcagc
     4621 gtcttgaagt gttgaataaa tcaaatgatg gattttatat agcaaatgaa gacttaaaac
     4681 ttcgaggacc gggtgatttc ttcggagtca gacaaagcgg gatgatggat tttgttctgg
     4741 cagatatcta taccaatgca gatattatga aacaggcagc tgatgcggta aaacaactgg
     4801 aggaaagcgg atttgatttt tcgaatttga agaacagcag attagaaaaa cagatcgggc
     4861 ttgcaaggaa tatataatag gaggtatatc gtatggtacg tttttattta agatttacag
     4921 gaagagtcca aggagtagga tttcgttttt ttgtagcgat gaatgcagaa agatgtcatt
     4981 tgacaggatg ggtgaaaaat atgtctgatg gatcagtgac agcagaagtt cagggaacca
     5041 gagttgaaat tgagaaattt ttaaatttga tccagacagg aaatcgtttc attcgagtgg
     5101 atgaaatcga gaaagaagaa cgtccatatg aagaaaatga gaaaagattt catgttcgtt
     5161 attaaaatga tgtaagtgtc aaaaaataac aatccataaa atgcaacatc atgtaaaaac
     5221 tcccagtgta tcttttgtac gcttgcatat actatgagcg taacagataa caaaacaaaa
     5281 gaaatggagg actttcaaca tggaagaacg aacttcagca
//
LOCUS       DAOMUE010000125         5302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4293_length_5302_cov_2.317705, whole genome shotgun sequence.
ACCESSION   DAOMUE010000125 DAOMUE010000000
VERSION     DAOMUE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4293_length_5302_cov_2.317705"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /gene="uppS"
                     /locus_tag="PLC71_06575"
     CDS             complement(<1..259)
                     /gene="uppS"
                     /locus_tag="PLC71_06575"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRM02340.1"
                     /translation="MENLNIPQHVAIILDGNGRWAKKRHLPRNMGHRQGSKVVEQIIE
                     DAHDLGIKYLTVYAFSTENWKRPQDEVNALMKLLRDYLKTCI"
     gene            complement(349..894)
                     /gene="frr"
                     /locus_tag="PLC71_06580"
     CDS             complement(349..894)
                     /gene="frr"
                     /locus_tag="PLC71_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRM02341.1"
                     /translation="MLKQFEDKMQKSLDSMANDFMTIRAGRANPHVLDKVKVDYYGVA
                     TPVAQVGNVSVPEARTIMIQPWEPNLLKEIEKAILVSDIGITPTNDGKAIRLNFPDLT
                     EERRKELAKDVKKRGEEAKVAVRNIRRDANDFVKKQNKAGDLNEDQAKDEEDKVQKMT
                     DKYIKQIDEAVDAKSKEIMTV"
     gene            complement(918..1613)
                     /gene="pyrH"
                     /locus_tag="PLC71_06585"
     CDS             complement(918..1613)
                     /gene="pyrH"
                     /locus_tag="PLC71_06585"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRM02342.1"
                     /translation="MKRVLLKLSGEALAGDKKTGFDEATCADAARQVKTLVDEGLQVA
                     IVIGGGNFWRGRTSESMERTKADQIGMLATVMNCIYVSDAFRTAGMETEVYTPFVCGA
                     FTELFSKDKAVKALDEGKVVFFAGGTGHPYFSTDTATALRAIEIEADAILLAKSIDGV
                     YDSDPAVNENAVKYDSITMEEVLDQKLGVIDLTATIMCLENKMPLAVFGLNEENSIEN
                     LMKGEFNGTYVTV"
     gene            complement(1750..2136)
                     /locus_tag="PLC71_06590"
     CDS             complement(1750..2136)
                     /locus_tag="PLC71_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02343.1"
                     /translation="MQNKTKNIFFKIISGLVLIQGVLQLLNLLGLVGYGRTTTSATKF
                     YLGLVLIGGYAVAAMVGGYTGLKDGNTQEGCKRCFYVSLVLIAITIATIILNVVFKAY
                     SVTQFEALIIPVLFMLFTILNGRKNL"
     assembly_gap    2214..2223
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2229..2579)
                     /locus_tag="PLC71_06595"
     CDS             complement(2229..2579)
                     /locus_tag="PLC71_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HRM02344.1"
                     /translation="MNIQEFYDSIDESYENVSSRMMGNQKLVEKFVRKFLDDPTYGQI
                     KEAVNKMDYDEILRTTHTMKGIASNLEFTHLQQKSAKAVDMIRAGQREEVLPVIGEIE
                     KEYQKVIEQIQKLD"
     gene            complement(2666..>5302)
                     /locus_tag="PLC71_06600"
     CDS             complement(2666..>5302)
                     /locus_tag="PLC71_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HRM02345.1"
                     /translation="LRDLDTFSYEQQANAPCPFCTNHCQRTIVQFSNGNSWVTNNRCE
                     KGEIIGDPRNDEVGKQLKETIRKTQSVPDLFQERKKLLFKEYPYPVPKTERDITIGIP
                     RVLSYWDTMPFWTTFFKSLGYKVQLSDESTREIYESGLSAVTSDTVCFPAKLVHGHLR
                     NLVKKYHVDRIFMPSITTVPPENMEKTSESMCAVVKGYPIVIRNSDNPQDKWNVPFDA
                     PLFHWHTKKDRQHQLVKYMEEVYHVSKEEVMNAMHTADQAQKSFDQQLKKRGEEILNE
                     VKEKNEFAVVLASRPYQNDTLVNHDLSKMFTKAGIAVLTADSLPEVNQTELSKSRLDI
                     VNNYHARMLSSAILCAQSEHLEYVQLVSFGCGHDAYLSDEITRMMKEISGKTPLILKV
                     DESDNQGPLGIRVRSFLETVKMGREKHQKLEVKELQEPYPVKFTKENRKEKIALVPNT
                     SHAFCRIMTAALRGQGIRAVALDIGREEAIRLGKKYVHNDICFPAQIVIGEALAALES
                     GKYDDKDVAIVMGKYVGDCRLTHYGALLRKALDDAGYDHIPILTNDDADSHNMHPGFK
                     LNLASSVKIAFALPMIDVLEELLRKIRPYETVKGSADEAFDKALDLVIDGLEKSGVLG
                     ARKGFKKAISIMKNISYDRTNLKPQILIVGEYLLNFHPGANHDIEKYLEENGFEIIEA
                     RMTDVIRKTYFYQDSQIREYHLNKPMDQKIWFRTADMFFDLAHSLTDSIAKGHPLYKP
                     AIRMDDLVKDSDPIIHHTFDAGEGVLIPGEIIHHAKHGCKYFLILQPFGCLPNHVVGR
                     GISKKLKEMYPNAQILPLDYDPDVSFANIENRLQMLVMNAKQEILEENEERDRRRSHH
                     YMESDKKTYRRKKYGVEKTSGV"
BASE COUNT         1414 a         1178 c          792 g         1908 t
ORIGIN      
        1 tgatacatgt ctttaaataa tcacgaagca gtttcatcaa tgcatttact tcgtcctgtg
       61 ggcgtttcca attctctgtt gaaaaagcat aaactgttaa atatttaatt ccaagatcat
      121 gtgcatcttc tatgatctgt tccaccactt tacttccctg tctatgtccc atatttctag
      181 gtaaatggcg tttttttgcc catcttccat tcccatctaa gataatggcc acatgttgtg
      241 gaatatttaa attctccatt tggacctcct gtgtatcata tataactttc gtttctttcc
      301 acatgaaaca aaacagggct gagatttcaa ccctgttgat gattaagatt aaactgtcat
      361 gatttctttt gattttgcat caactgcttc atcaatctgt ttgatgtatt tatctgtcat
      421 cttctgtact ttgtcttctt catcttttgc ctgatcttca tttaaatctc cggctttatt
      481 ctgtttcttg acaaaatcat ttgcatcacg acggatattt ctaactgcta cctttgcttc
      541 ttctccacgt ttcttgacat cttttgcaag ctcttttctt cgttcttctg taagatctgg
      601 gaagtttaag cggattgctt taccatcatt tgttggtgtg atcccgatat cagatactaa
      661 aatcgctttc tcgatctctt ttaataagtt tggctcccat ggctgaatca tgatcgttct
      721 tgcttcaggt acagaaacat taccaacctg tgctacagga gttgcaactc cataataatc
      781 aactttgact ttatctaata catgaggatt ggcacgtcct gcacgaattg tcataaaatc
      841 attcgccata ctgtctaaag atttctgcat cttgtcttca aattgtttta acatgtttct
      901 ttcgtcctcc taatttatca aacggttaca tatgttccgt taaattcacc tttcattaaa
      961 ttctcaatgc tgttttcttc atttaatcca aaaactgcta atggcatctt attttcaagg
     1021 cacatgatcg ttgctgttag gtcgatcact cctaatttct gatccaacac ttcttccatt
     1081 gtgatactgt catatttcac cgcattttca ttcacagcag gatcactgtc atatacgccg
     1141 tcaattgatt ttgctaataa gatcgcatct gcttcaatct cgatcgcacg aagtgctgtt
     1201 gctgtatctg ttgagaaata aggatgtcct gttccacctg caaaaaatac aacctttcct
     1261 tcatctaaag ctttgactgc tttatctttg gaaaataatt ctgtaaatgc tccgcataca
     1321 aatggagtat atacttctgt ttccattcct gctgttctga atgcatcgga tacatagata
     1381 cagttcataa ctgtcgcaag cattccgatc tgatcagctt ttgtacgttc catactctcg
     1441 cttgtacgtc ctctccagaa gtttcctcct ccgatgacga tcgctacctg aagtccctca
     1501 tctactaatg tctttacctg acgtgctgca tctgcacatg ttgcttcatc aaatcctgtc
     1561 ttcttatctc cggcaagtgc ttctccactt aactttaaaa gcacacgttt catatctttt
     1621 aaatctttca ttggtctacc tcatttgttc tttctttgcc aaatcactat gtgaaattat
     1681 atcatatcat caagaaaaga acaactataa aaaggacctc tttcgaaatc gaaaaagacc
     1741 cttttgtgtt tacaaattct ttcgtccatt taatattgta aaaagcataa acaacactgg
     1801 tatgatcagt gcctcaaact gagttactga atatgcttta aatacaacat ttaaaataat
     1861 cgtggcaatc gtaattgcga tcagtacaag actgacataa aaacatcttt tacatccttc
     1921 ctgtgtattt ccatctttaa gacccgtata tccaccgacc attgctgcta ctgcgtagcc
     1981 gccgatcaaa acaagtccaa gataaaactt tgttgcagaa gtcgtcgttc ttccataacc
     2041 tacaagccct aacagattca ataactgcaa cacaccctga atcaaaacga gtcctgagat
     2101 tatcttaaaa aatatatttt ttgttttgtt ttgcataatc gctagttcct ttttattttt
     2161 tatgttgtcg ctgtctcctt catactactt cgaaagattc cctcatcttt gtcnnnnnnn
     2221 nnnccaattt aatccaattt ctggatctgt tcgataactt tttgatattc tttctcaatt
     2281 tcaccgatca ctggcaatac ttcttcgcgc tgtcctgcac ggatcatatc tactgctttc
     2341 gcactttttt gctgtaaatg tgtaaactcc agattgcttg caattccttt catcgtatga
     2401 gttgttcgaa gaatttcatc ataatccatc ttatttactg cttctttaat ctgtccataa
     2461 gtaggatcat ctaaaaactt acggacaaac ttttccacca gtttctggtt tcccatcata
     2521 cggctgctta cattttcata actttcatca atactgtcat aaaactcctg aatattcatt
     2581 acttctcctc cattcaaatt atttttgaat aacctaaaat ggattatagc atatttaaca
     2641 aaattcaaca atttaattct atattttata caccagaagt tttttcgaca ccatatttct
     2701 ttctacgata tgtttttttg tcactttcca tgtagtgatg acttcttcta cggtctcgtt
     2761 cttcattttc ttccaagatt tcttgtttcg cattcataac aagcatctgc agacgatttt
     2821 cgatattcgc aaaactgaca tctggatcat agtctaatgg aagaatctgt gcatttggat
     2881 acatttcttt taatttttta gaaatacctc ttccaacaac atggtttggc aggcatccaa
     2941 atggctgtaa gatcaagaaa tacttgcatc catgttttgc gtgatgaatg atctctcctg
     3001 gaatcagcac accctctcct gcatcaaatg tatgatgaat gatcggatca ctgtctttta
     3061 ccagatcatc catacggatc gctggtttat ataaaggatg tccttttgca atggaatcgg
     3121 tcagactgtg tgcaagatca aagaacatgt ctgctgtccg aaaccagatc ttttgatcca
     3181 ttggtttatt taaatgatat tccctgatct gtgaatcctg ataaaagtat gtcttacgga
     3241 taacatccgt catacgtgct tcaatgatct caaatccatt ttcttccaga tatttttcaa
     3301 tgtcatggtt tgctcctgga tgaaagttca gaagatattc cccaacgatc agaatctgtg
     3361 gttttaagtt cgtacgatca taagaaatat ttttcatgat agaaatagcc ttcttaaatc
     3421 cttttcttgc accaagaact cctgactttt ccaatccatc gattacaaga tcaagggctt
     3481 tgtcaaacgc ttcatccgca cttcctttga ctgtttcata tggacggatc tttcgtaata
     3541 attcctccaa tacatcgatc atcggtaatg caaatgcgat ttttacagaa gatgccagat
     3601 ttaatttaaa tcctgggtgc atattatgag aatctgcatc atcatttgtc aggatcggaa
     3661 tatgatcata tcctgcatca tcaagagctt ttcttagtag tgccccataa tgtgtcagtc
     3721 gacagtcccc aacatatttt cccataacga tcgcaacatc tttgtcatca tactttccac
     3781 tttccagtgc cgccaatgct tctccgatga cgatctgtgc tgggaaacag atatcattat
     3841 gtacatattt ctttcccaaa cgaattgctt cttcacgccc gatatccaaa gcaaccgccc
     3901 gaattccttg tcctcttaat gctgctgtca taattctgca aaatgcatgc gatgtatttg
     3961 gaaccaatgc gattttttct ttcctgtttt cttttgtaaa cttgactgga tatggttctt
     4021 gcaactcttt tacttctaat ttctgatgtt tctcacgtcc cattttcact gtctccaaga
     4081 aagaacgcac acggattccc aatggtcctt gattatcact ttcatctact tttaagatca
     4141 atggtgtttt cccggaaatt tctttcatca ttctcgtgat ctcatcggat agataagcat
     4201 catgtccaca tccaaaactt acgagctgta catattccag atgctcactt tgcgcacata
     4261 agatagcaga agataacatt ctagcatgat agttatttac aatatccaga cggctttttg
     4321 agagttctgt ctgattcact tctggcaggg aatctgcggt taagactgcg atgcctgctt
     4381 ttgtaaacat cttagacaga tcgtgattaa ccagtgtatc gttctgatat ggacgagatg
     4441 ctaaaacaac tgcaaattca ttcttttcct ttacttcatt tagaatctct tctcctcgtt
     4501 tctttaactg ttggtcaaaa gatttctgag cctgatctgc tgtatgcatt gcattcataa
     4561 cctcttcttt tgagacatga tatacttctt ccatatattt taccaactga tgctgtctgt
     4621 cctttttggt atgccaatgg aataatggtg catcaaatgg cacattccat ttatcctgtg
     4681 gattatcaga attcctgata acgatcggat aaccttttac caccgcacac atagactcac
     4741 ttgttttttc catattctct ggcggtactg ttgtgatcga tggcataaaa atacgatcta
     4801 catgatattt tttcaccaga tttctaagat gtccatgcac aagttttgct ggaaaacata
     4861 ccgtgtctga tgtcactgct gacaatccac tttcataaat ctctcttgtg ctttcatccg
     4921 ataattgtac tttgtatccc aaacttttga aaaaagttgt ccaaaatggc atcgtatccc
     4981 aataggataa cactcttgga attccgatcg tgatatctct ttctgtttta ggcactggat
     5041 aaggatattc tttgaacaat aatttctttc gctcttggaa taaatctgga acactctgtg
     5101 tttttcggat tgtttctttt aactgctttc caacctcatc atttcttgga tctccgatga
     5161 tctcgccttt ttcacatcga ttatttgtta cccaggaatt tccatttgaa aattgaacaa
     5221 ttgttctttg acaatgattc gtacaaaatg gacaaggtgc attcgcctgc tgctcataag
     5281 aaaatgtatc taaatcacga ag
//
LOCUS       DAOMUE010000126         5292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4299_length_5292_cov_3.048883, whole genome shotgun sequence.
ACCESSION   DAOMUE010000126 DAOMUE010000000
VERSION     DAOMUE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4299_length_5292_cov_3.048883"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..1005
                     /locus_tag="PLC71_06605"
     CDS             205..1005
                     /locus_tag="PLC71_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRM02346.1"
                     /translation="MGFLKGKTAIITGGGRSVLKDGSCGSIGYGIATAYAKEGANLVI
                     TGRNKQKLADAKEELERLYNIEVLPVQADVSAGADNETVVQNVIDETIAKFGRIDVLI
                     NNAQASASGVTLADHTTEQFDLALYSGLYAAFYYMKACYPHLKETKGSVINFASGAGL
                     FGNYGQCAYAAAKEGIRGLTRVAANEWGPDGINANIICPLAWTAQLENFQQAYPEAFE
                     ANVKMPPAGHYGDVEKEIGRVCVQLASPDFKYMNGETLTLEGGMGLRP"
     gene            complement(1154..3193)
                     /gene="ftsH"
                     /locus_tag="PLC71_06610"
     CDS             complement(1154..3193)
                     /gene="ftsH"
                     /locus_tag="PLC71_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRM02347.1"
                     /translation="MNNQQNNKNDKNDKDNKKKNAKILAIFLIVSILGTFFFNSILTK
                     WRNGTEKTISYNKFVTMLDKDQVKSVKVTDSQLKVTPKNEGNPFYKTTYTVQRISGDY
                     ELVNRLIKSKIAFEQEPEDPMDTFLSILISLIPTIVIFGFLVMMMRRSSGMMGVGKSN
                     AKVYVEKKTGVTFADVAGQGEAKETLTEMVDFLHHPERYTEIGAKLPKGALLVGPPGT
                     GKTLLAKAVAGEAGVPFFSLSGSDFVEMFVGVGASRVRDLFKQAQQMAPCIIFIDEID
                     AIGKSRDNGMHGGGNDEREQTLNALLAEMDGFDTSKGIIILAATNRPEVLDKALLRPG
                     RFDRRVIVERPDLKGRIETLKVHAKDVKMDETVDFEEIALATSGAVGSDLANMINEAA
                     LGAVKAGRKAVSQKDLFEAVEVVIAGKEKKDRILGDEEKHIVAYHEVGHALVMALQKE
                     SEPVQKITIVPRTMGALGYTMQRPEEEKYLNKKDEMLADLVSFFGGRAAEEVKFNSVT
                     TGASNDIERATAIARAMVTQYGMSDEFGLIGLESITNRYLDGRAVMNCAETTAAKVDE
                     VVMKMLKEAYAKAVTYIRENMDILDEAAEFLIQRETITGKEFMEIFNKYKNPVEESDK
                     NVSETDENVSETDENDAETPGPEVKTDSEIKTEEENTSTYNTGYAQWVEDDDDEE"
     gene            complement(3281..3685)
                     /locus_tag="PLC71_06615"
     CDS             complement(3281..3685)
                     /locus_tag="PLC71_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HRM02348.1"
                     /translation="MKQRIWIFIFGIILCVILTGCIKKDERGKKQKVVYVVCRRSVIP
                     VQLGKLIDEQKREAFHFTYTTGGFTYYVIGYGRQPGSGYKIKVREFSADRTHIYIDTI
                     LTGVTKEHQKHGKSYPYIVLKSQFYEKDVIFH"
     gene            complement(3723..5027)
                     /locus_tag="PLC71_06620"
     CDS             complement(3723..5027)
                     /locus_tag="PLC71_06620"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HRM02349.1"
                     /translation="MRMYDLILKKRQGKPLTKDEIQWMIREYTDGRIPDYQMSALLMA
                     ICFQGLDKEETYELTMAMARSGEMLDLSQIQGIKVDKHSTGGVGDKTSLALTPIVASL
                     GIPVAKMSGRGLGHTGGTIDKLESIPGFSTQLEDDAFEEQVNSIGISIMGQTKDLAPA
                     DKLLYALRDVTATVDQISLIASSIMSKKLAAGADAILLDVKTGSGAFMKEESDAIQLA
                     KEMVEIGKSAGRKMTAVITNMDEPLGMAVGNILEVKEAIDTLKGNGPDDFVELIETLA
                     SHMLILGGAAKDFDEGLMRVRESIQSGKALDKFKQFVAAQGGDTKYIDHPELFEQADI
                     IEEIRSEQDGFVGSIDAQEMGICSLILGGGRETKESVIDPTVGLVFSKKVADPVKKGD
                     VLATIYGNDEEKVKQAVLHFKENYHIIEEKPTKPIMIREVLS"
     gene            5097..>5292
                     /locus_tag="PLC71_06625"
     CDS             5097..>5292
                     /locus_tag="PLC71_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02350.1"
                     /translation="MTEIKEKQIFERIRWQLRIFESVFYKSLIRACSFGNFLAASQNA
                     RSNGQTSNHRHCEFVKNALKK"
BASE COUNT         1412 a         1249 c          882 g         1749 t
ORIGIN      
        1 ctcatgaatg ctacatcttc aggatctaat tctaattctg cagatttttt tatgttgttc
       61 caaatttagt tcattctaga tcggaatctt ttcgttaatt aataaattaa cataattgtc
      121 agataattaa cgaaattatt ccttgattcc acacaatttg tctgttatga ttgtctcatc
      181 acttaataat gagaggtaaa acttatggga tttttaaaag gtaaaacagc catcatcact
      241 ggtggcggaa gatctgtatt aaaagatgga agctgtgggt ctattggtta tggaattgca
      301 actgcatacg caaaggaagg tgcaaacctc gttattacag gaagaaataa acagaaactt
      361 gccgatgcca aagaggaact cgagagatta tacaatatcg aagttctacc agtccaagcc
      421 gatgtaagtg ccggtgctga caacgagaca gtcgtgcaga atgtcattga tgaaacaatc
      481 gccaaattcg gacggatcga tgttctgatc aataatgcac aggcatctgc ttctggagtt
      541 actttagctg atcatacgac tgaacaattt gatctcgcac tttattccgg tctttatgcc
      601 gcattttact atatgaaagc atgttaccct catttgaaag agacaaaagg ctctgtcatt
      661 aactttgcat ccggtgccgg attatttgga aactatggtc aatgtgctta tgctgcagca
      721 aaagaaggaa tccgtggtct taccagagtt gccgcaaatg aatggggtcc tgacggcatc
      781 aatgctaata tcatttgtcc actcgcatgg acagcacagc ttgaaaattt ccagcaggct
      841 tatcctgaag cctttgaagc taatgttaaa atgcctcctg caggacatta tggagatgtt
      901 gagaaagaaa tcggaagagt atgcgttcag ttagcttcgc ctgatttcaa atatatgaac
      961 ggagaaacac ttaccttaga aggtggaatg ggattaaggc cgtaacaaga cctgctgagg
     1021 atagaatgta ttttttacct ttggcatcct tttatatcag ctatatggaa aaatgcggag
     1081 acattcgttt aatcgatcgt ctccgcattt ttatgctttt cgctatttta ttttgttcac
     1141 tgactctgac agattattct tcatcgtcat catcttccac ccactgggca tatccagtat
     1201 tatatgtcga tgtattttct tcttctgtct tgatctcaga atctgttttt acctctggcc
     1261 ctggagtttc tgcatcattt tcatctgttt ctgatacatt ttcatctgtc tctgatacat
     1321 ttttatcaga ctcttctact ggattcttgt atttgttgaa gatttccatg aattcttttc
     1381 ctgtgatcgt ctcacgctgg atcaaaaact ctgctgcttc gtcaagaatg tccatatttt
     1441 cacgaatgta tgtgactgct ttggcgtaag cttcttttaa catcttcatg acaacttcat
     1501 cgactttcgc tgctgttgtc tctgcacagt tcataactgc acgtccatct aagtatcggt
     1561 ttgtaatgga ctctaaaccg atcaatccaa attcatcgga cataccatac tgtgttacca
     1621 tcgctcttgc gatcgctgtg gcacgctcaa tatcattgga tgctcctgtt gtgacagagt
     1681 taaacttcac ttcttctgcc gcacgtccac cgaagaatga gacaaggtct gctaacatct
     1741 catccttctt atttaagtat ttttcttctt ctggacgctg cattgtatat ccaagtgctc
     1801 ccatggtacg aggtacgatg gtgatcttct gtactggttc tgattctttt tgcaatgcca
     1861 taaccagtgc atgtccgact tcatggtatg caacaatgtg tttctcttca tcgccaagaa
     1921 ttctgtcctt cttctcttta ccagcaatga caacttccac tgcttcaaat aagtctttct
     1981 gtgaaacagc ctttcttcct gccttgacag ctccaagtgc tgcttcgttg atcatattgg
     2041 caagatcaga tccaacagct ccactggttg ccagtgcgat ttcttcaaag tcaacagtct
     2101 catccatctt cacatctttg gcatgtactt ttaatgtctc aattcttccc tttaaatctg
     2161 ggcgttctac aataacacgg cggtcaaaac gtcctggacg aagaagggct ttgtctaaga
     2221 cttctggacg gtttgttgct gcaagaataa tgataccttt ggatgtatca aatccatcca
     2281 tctctgcaag taaagcattc aatgtctgtt cacgttcatc atttcctcca ccatgcattc
     2341 cattatcacg actcttacca atagcgtcga tctcatcaat aaaaataata catggtgcca
     2401 tctgctgtgc ctgtttaaat aagtcacgaa ctctggatgc tccgactccg acaaacatct
     2461 ctacaaagtc agatccagaa agtgaaaaga atggaactcc tgcttctcct gctactgctt
     2521 tggcaagcaa tgtcttacca gtacccggag gtcctacaag caatgcacct tttggcagct
     2581 tcgcaccaat ctctgtgtat cgttctgggt gatgaagaaa atctaccatc tctgtcagtg
     2641 tttccttcgc ttctccctgt cccgcaacat ctgcaaatgt cactcctgtc ttcttctcaa
     2701 catatacctt cgcattggac tttccaaccc ccatcattcc gctgcttctt cgcatcatca
     2761 tgacaaggaa tccaaagatc acgatcgtag ggatcaaact gatcaatatc gataagaaag
     2821 tatccatcgg atcttctggt tcctgttcaa atgcgatctt actcttgatc agacgattca
     2881 ccagttcata atctccgctg atcctctgta ccgtatatgt tgtcttgtaa aaaggatttc
     2941 cctcgttctt tggtgtcact ttcaactggc tgtctgtgac ctttacactt tttacctgat
     3001 ctttatcaag cattgtgaca aatttattgt acgaaatcgt tttctccgtt ccgtttctcc
     3061 attttgttaa aatcgaatta aaaaagaaag taccaagaat tgaaacgatc aggaagattg
     3121 caagaatctt cgcgtttttc ttcttgttat ctttatcatt tttatcattc ttattattct
     3181 gttgattatt caaatgtcag ctcctccttt gattcataag caccattata tgttttctga
     3241 ttttataaat caataaaact attctaaact ttttataatt ttagtgaaaa atcacatctt
     3301 tttcgtaaaa ctggcttttt agtacaatat atggataaga ttttccatgc ttctggtgct
     3361 cttttgtaac ccctgtcagt atcgtatcaa tataaatatg tgtccggtct gcactaaatt
     3421 ctctaacctt gatcttatag ccgctccccg gctgtcttcc atatccgatc acataataag
     3481 taaaaccacc tgttgtatat gtaaaatgaa atgcttccct cttttgttca tcgatcagtt
     3541 tgcctaattg taccgggatc acacttcttc tacagacaac atatacaacc ttctgttttt
     3601 tccctcgttc atcctttttg atacatcctg tcaaaatcac acataaaata ataccaaaaa
     3661 taaatatcca gattctttgt ttcatggatc tctatccttt ttatcatttt atgcaaatag
     3721 atttaagata atacttcccg gatcattatc ggtttcgttg gtttttcttc tattatatgg
     3781 tagttttctt taaaatgaag aactgcctgt tttacttttt cttcatcatt tccataaatc
     3841 gttgcaagca catcgccttt cttcacagga tccgcaacct tctttgagaa aacaagtccg
     3901 accgttggat cgatcacact ttccttcgtt tctcttcctc caccaaggat caaagagcag
     3961 attcccattt cctgtgcatc aatacttccg acaaatccat cttgttctga tcgtatttct
     4021 tctataatat ctgcctgttc aaataactct ggatgatcga tgtatttcgt atcaccacct
     4081 tgtgcagcaa caaattgttt gaatttatcc aatgctttcc cactttgaat agattcccgg
     4141 actctcataa gtccttcatc aaaatccttt gctgcaccac caaggatcag catatgcgaa
     4201 gccagtgtct caataagctc cacaaaatca tctggtccat tgccttttaa tgtgtcgatc
     4261 gcttctttga cttccagaat atttccaact gccattccaa gaggttcatc catattcgtg
     4321 atcacagccg tcatcttcct tccggcactc tttccgatct ctaccatctc tttggcaagt
     4381 tgtatcgcat ccgactcttc tttcataaat gcaccgcttc cggtctttac atccaacaag
     4441 attgcatctg ctcctgctgc cagcttctta ctcataatac tgcttgcgat caaagaaatc
     4501 tgatccaccg ttgcggtcac atcccgcaat gcatacaaca atttatctgc tggtgcaaga
     4561 tccttcgtct gccccatgat tgaaatacca attgaattca cttgttcttc aaacgcatca
     4621 tcttcaagct gtgttgaaaa accaggaata ctctccagtt tatcgatcgt acccccagta
     4681 tgaccaagtc cacgcccaga catcttagca accgggattc caagcgaagc aacaatcggt
     4741 gtcaacgcca aagaagtctt atctccaacc cctcccgtac tatgcttatc aaccttgatt
     4801 ccctgaatct gagacagatc aagcatctca ccacttcgcg ccattgccat cgtaagctca
     4861 taagtttctt ctttatcaag cccttgaaaa cagatcgcca tcaacaaagc tgacatctga
     4921 taatccggta tcctgccatc tgtgtattca cggatcatcc attgaatctc atctttcgtc
     4981 aaaggtttcc cttgtctctt ttttagtatc agatcataca ttctcataga ttcggcctcc
     5041 tttgcttttt gttgtatata gtataacaca aaatttgtag gtaggcttac gtgtatgtga
     5101 cagaaataaa agaaaaacaa atatttgaaa gaatcaggtg gcaactccgc atttttgaga
     5161 gcgtttttta caaatcgctg atccgtgcct gttcctttgg aaacttcctc gctgcgagcc
     5221 aaaatgcccg cagcaacggt cagacatcca accacaggca ctgcgaattt gtaaaaaacg
     5281 cactcaaaaa gt
//
LOCUS       DAOMUE010000127         5283 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4308_length_5283_cov_3.075746, whole genome shotgun sequence.
ACCESSION   DAOMUE010000127 DAOMUE010000000
VERSION     DAOMUE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4308_length_5283_cov_3.075746"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..839)
                     /locus_tag="PLC71_06630"
     CDS             complement(135..839)
                     /locus_tag="PLC71_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HRM02351.1"
                     /translation="MKNKIKVLSGAQLKYIAFLSMLIDHTNKALIYPNLNGGRLNTVS
                     DIFDIIGRIAFPIFAFLLVEGFFKTRSRAKYLATLLVFGVISEVPFDLFTTKQFFEPN
                     WNNIMFTLALVLVTIWMIDVLKKKMEKFPKILWFLLSFVILAVMCLIAAILSLDYDYH
                     AILIGYFYYIFHGRELIAIPFNFLSMYKEPWALLGFGLVLTYNGERGKQNKLINYLFY
                     PVHLLILGLLRIYLGI"
     gene            1402..1725
                     /gene="rpsJ"
                     /locus_tag="PLC71_06635"
     CDS             1402..1725
                     /gene="rpsJ"
                     /locus_tag="PLC71_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRM02352.1"
                     /translation="MAASQVMRITLKAYDHQLIDASAKSIIETVKKKTGSKVSGPVPM
                     PTKKEIITILRATHKYKDSREQFEQRTHKRLIDIITPSQKTVDALSKLEVPAGVYIDI
                     KMKNK"
     gene            1842..2471
                     /gene="rplC"
                     /locus_tag="PLC71_06640"
     CDS             1842..2471
                     /gene="rplC"
                     /locus_tag="PLC71_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRM02353.1"
                     /translation="MKKAILTTKVGMTQIFNEDGVLTPVTVLQAGPCVVTQVKTEEND
                     GYSAVQVGFVDKKEKGINKPQKGHFDKAGVAPKRFVREFRFENAEEYTVGQEIKADIF
                     AAGDKIDATATSKGKGYAGGIKRHGLKSGPSAHGSKYHRHAGSNGMASDPSKVFKGKK
                     MPGQMGAERVTIQNLEIVKVDADQNIILVKGAVPGPKKSLVMLKESVKA"
     gene            2501..3121
                     /gene="rplD"
                     /locus_tag="PLC71_06645"
     CDS             2501..3121
                     /gene="rplD"
                     /locus_tag="PLC71_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRM02354.1"
                     /translation="MATVSVLNMEGKEVEKLELNDNIFGVEINEHLVHLAVVSQLANN
                     RQGTQKAKTRSEVRGGGRKPWRQKGTGHARQGSIRAPQWKGGGVVFAPTPREYTKKLN
                     KKERRIALKSALTSRVQADKFVVIDEFKFDEIKTKNVKAMLDSVGAKKALVVMGSKND
                     NLVLSARNLPGVKTALTSTINVYDILKYDTVVVSKEAVANIEEVYA"
     gene            3121..3420
                     /gene="rplW"
                     /locus_tag="PLC71_06650"
     CDS             3121..3420
                     /gene="rplW"
                     /locus_tag="PLC71_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRM02355.1"
                     /translation="MADIKYYDVILKPVVTEKSMNAMAEKKYTFLVHPSATKNQIKEA
                     VEKMFEGTKVAKVNTMNNDGKTKRRGMTFGKTAKTKKAIVQLTEESADIEIFEGL"
     gene            3510..4355
                     /gene="rplB"
                     /locus_tag="PLC71_06655"
     CDS             3510..4355
                     /gene="rplB"
                     /locus_tag="PLC71_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRM02356.1"
                     /translation="MGIKSYNPYTPSRRHMTGLDFDVITKSTPEKSLVVSLKKNAGRN
                     NQGKITVRHRGGGSRRKYRLIDFKRNDKDGIPATVAAIEYDPNRTANIALLVYADGEK
                     RYIIAPNKLAVGTTVMNGPEAEIKVGNCLPLANIPVGTEIHNIEMHPGKGGQLVRSAG
                     NSAQLMAKEGKYATLRLPSGEMRMVPVVCRATIGQVGNIEHDLVNIGKAGRKRNMGIR
                     PTVRGSVMNPNDHPHGGGEGKTGIGRPGPCTPWGKPALGLKTRKKNKQSNKMIVRRRD
                     GKTVK"
     gene            4372..4653
                     /gene="rpsS"
                     /locus_tag="PLC71_06660"
     CDS             4372..4653
                     /gene="rpsS"
                     /locus_tag="PLC71_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRM02357.1"
                     /translation="MARSLKKGPFADKSLLNKVDAMNEAGDKSVIKTWSRRSTIFPSF
                     VGHTIAVHDGRKHVPVYVTEDMVGHKLGEFVATRTFRGHKKTEKKTGMK"
     gene            4681..5070
                     /gene="rplV"
                     /locus_tag="PLC71_06665"
     CDS             4681..5070
                     /gene="rplV"
                     /locus_tag="PLC71_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRM02358.1"
                     /translation="MAKKYSRSQYKKERNANKDTRPKATLRNARVSTTKAAFVLDAIR
                     GKDVETALGILLYNPRYASTLIEKLLKSAIANAENNNGMDPENLYIAECYANKGPTMK
                     RIKPRAQGRAYRIEHRMSHITVVLDER"
     gene            5087..>5283
                     /locus_tag="PLC71_06670"
     CDS             5087..>5283
                     /locus_tag="PLC71_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRM02359.1"
                     /translation="MGQKVNPHGLRVGIIKDWDSRWYAEDAFADNLVEDDKIRKYVKK
                     RLYNAGISKIEIERASDRVKL"
BASE COUNT         2014 a          854 c         1068 g         1347 t
ORIGIN      
        1 tagtgttgca tgtatgagtc agaaagaaga taagaagaaa tatttataag aaaaaagggt
       61 gacactctgc atttttgaaa gcgatttgta aaaacgcatt caaaaagtgc tcatgttgtc
      121 acccttttct ctacttatat tcctaagtat attcttaaca gtccaagtat cagcaagtgg
      181 acaggataaa ataaataatt gatcagctta ttctgttttc cacgttctcc gttatacgta
      241 agtaccaatc caaatccaag aagtgcccat ggttctttat acatggataa gaaattaaat
      301 ggaattgcaa taagttccct tccatggaaa atataataaa aatatccaat taaaattgca
      361 tgataatcat agtcaaggct gagaatggca gcaatcaggc acatgaccgc caggatcaca
      421 aatgacaaga gaaaccataa tatcttagga aacttctcca ttttcttttt taacacgtcg
      481 atcatccaaa ttgtaacaag tacaagtgct aacgtaaaca taatgttatt ccagtttggt
      541 tcaaaaaatt gctttgttgt aaacagatca aaaggaactt ctgaaattac cccgaatact
      601 aataaagttg caaggtattt agctctgctt cttgttttaa aaaaaccttc tacaagtaaa
      661 aatgcaaaga ttgggaatgc aattctcccg ataatgtcaa atatgtcact aactgtattc
      721 aatcggccac catttaaatt tggatagatc aatgccttat ttgtgtggtc gatcagcatt
      781 gataaaaatg caatgtattt taattgtgct cctgataata ctttaatttt attttttatg
      841 gctcttacct ccgtttctat atattgataa tgattaccgt tatcaaataa atgataatat
      901 atgaaattaa aaaagtcaat aacagtttat attatacata ttggaattgc aaaacgagtt
      961 ttttccatat ataataagga aagaactcgt tttcatttat gcaaaaaaat ataaaaaaca
     1021 agattatctt gtgcctaaaa cggtaatact catacagaaa acactacatt ttgtatttta
     1081 tcaaaaacat gcaaaaaaaa tcgaaaaacc ccttgcatta atttgaaaca tcatatataa
     1141 tagcaaatgt tgtgaccttg atagcgttga agcgtgaggt tgctgtagag gcaaaagcct
     1201 ttgcaggttt tccgtggagc gaatgtcaag ttgttaaaac tggcgacaag tcactgtacg
     1261 aatatacaat ctattgaaca ccggtgtagt ccggaataga gacgcagtgt gtagatttca
     1321 cgatacacac ggaggagtgt acagtcacca cttgtcgtgc tacaaaaaca tttatagtac
     1381 gaaaaggagg cggctttttt tatggcagca agtcaggtaa tgagaattac attaaaagca
     1441 tacgatcatc agttaatcga tgcaagcgca aaaagcatta ttgaaacagt taaaaagaag
     1501 acaggatcta aggtgagtgg accagttcca atgccaacta agaaagaaat catcacaatt
     1561 cttagagcga ctcacaaata taaagattcc agagagcagt tcgaacagag aactcacaaa
     1621 agactgattg atatcatcac accatcacag aaaacagttg atgcattatc taaattagaa
     1681 gtaccagctg gtgtgtacat tgatatcaag atgaaaaaca aataggtaat ccctacaggg
     1741 ttatgagtaa atgaggtatc cccttaagct tgtagcgtct aggatgatta caacgttaag
     1801 cgttataatc cgctgtagat taaataggag gaacaatcaa aatgaaaaaa gcgattttaa
     1861 caacaaaagt tggaatgact cagattttca acgaagatgg agttttaact ccagttactg
     1921 tattacaggc aggaccttgt gttgttactc aggtaaagac agaagagaat gacggataca
     1981 gcgcagttca ggttggtttt gttgacaaga aagaaaaagg aatcaacaaa ccacagaaag
     2041 gacacttcga taaagcagga gttgcaccaa aaagattcgt aagagaattc agatttgaga
     2101 atgctgaaga atacacagta ggtcaggaaa tcaaagctga catcttcgca gcaggagaca
     2161 aaatcgatgc aactgctaca tcaaaaggta aaggatatgc tggtggtatc aaacgtcacg
     2221 gtttgaaatc aggtccatct gcccatggtt ctaaatatca tcgtcatgca ggttctaatg
     2281 gtatggcttc tgatcctagc aaagtattca aaggaaagaa aatgccagga caaatgggag
     2341 cagaaagagt tactattcag aatcttgaaa tcgtaaaagt tgacgctgat cagaacatta
     2401 ttttagttaa aggtgctgta ccaggaccta aaaaatcttt agtaatgtta aaagaatcag
     2461 taaaggcgta ggtctttaga agaaaggagg actatataag atggcaacag tatctgtatt
     2521 aaatatggaa ggcaaagaag ttgagaaatt agaattaaat gacaacatct ttggcgttga
     2581 aataaatgaa catttagtac atctggcagt agtaagccag ttagcaaata accgtcaggg
     2641 aacacagaaa gcaaaaacac gttctgaagt tcgcggtggt ggaagaaaac cttggagaca
     2701 gaaaggaaca ggtcatgcaa gacagggatc tatccgagct ccacagtgga aaggcggagg
     2761 agttgtattt gctccaacac caagagagta cacaaagaaa ttaaacaaaa aagaaagacg
     2821 tattgcttta aaatctgcat taacatctag agtacaggca gacaaattcg tagtgatcga
     2881 cgaattcaaa ttcgacgaga tcaaaactaa gaacgtaaaa gcaatgcttg acagcgttgg
     2941 tgctaagaaa gcattagtgg ttatgggatc taaaaatgat aacttagtat tatctgcaag
     3001 aaaccttcca ggagttaaaa ctgctttaac aagcacaatc aatgtatatg acattttaaa
     3061 atatgacact gtagttgttt ctaaggaagc agttgcaaat atcgaggagg tatatgcata
     3121 atggcagata ttaaatatta tgacgttatc ttaaagccag ttgtaacaga aaaaagtatg
     3181 aacgcaatgg ctgaaaagaa atatactttt ttagttcatc catctgctac taagaatcag
     3241 atcaaagaag cagtggaaaa aatgttcgaa ggaacaaaag tagcaaaagt caacacaatg
     3301 aacaatgatg gaaaaacaaa gagacgtgga atgacttttg gaaaaactgc aaagacaaag
     3361 aaagctatcg tacagcttac tgaagaaagt gcagatatcg aaatcttcga aggactatag
     3421 gtattgacca cttagttcga ctaagataag gacttgaaac tatacgcaac aaaagcgtga
     3481 tattaaatca gatttgaaag gagactgaca tgggaattaa atcatataac ccatatacac
     3541 cttctagaag acatatgact ggacttgatt ttgatgttat tacaaaatct acaccagaaa
     3601 aatctctggt agtttcttta aagaagaacg ctggtcgtaa caatcagggt aaaattactg
     3661 taagacaccg cggaggtgga tctagaagaa aatacagact tattgacttt aagagaaatg
     3721 ataaagatgg tattccagca acagttgctg caatcgaata cgatccaaac agaacagcta
     3781 acatcgctct gttagtttat gcagacggtg aaaaaagata tattatcgct ccaaacaaat
     3841 tagcagttgg aacaactgtt atgaatggac cagaagctga aattaaagta ggaaactgcc
     3901 ttccattggc aaacattccg gtaggtacag aaatccataa catcgagatg catcctggaa
     3961 aaggtggaca gttagttcgt tccgcaggaa actcagcaca gttaatggca aaagaaggaa
     4021 aatatgcaac acttcgttta ccatctggtg agatgagaat ggtgcctgta gtatgccgcg
     4081 ctactattgg tcaggttgga aacatcgaac atgatcttgt gaacattggt aaagctggac
     4141 gtaaacgtaa catgggaatc cgtcctacag tccgtggttc tgttatgaac cctaatgacc
     4201 atccacacgg tggtggtgaa ggtaagactg gaatcggtag accaggacca tgcacaccat
     4261 ggggtaaacc agcacttgga ttgaagacac gtaagaaaaa caaacaatct aacaagatga
     4321 tcgtaagaag aagagatggt aaaactgtta aatagtacaa ggaggtaact tatggctcgt
     4381 tcacttaaaa aaggaccatt tgcagataag agtttactga acaaagtaga cgctatgaac
     4441 gaagcaggag acaagagtgt tatcaaaaca tggtctcgtc gttctacaat cttcccatca
     4501 ttcgtaggac acacaatcgc tgttcatgat ggaagaaaac atgtaccggt atacgttaca
     4561 gaagatatgg ttggacacaa acttggagag tttgtagcaa caagaacttt ccgtggacat
     4621 aagaaaacgg aaaagaaaac cggaatgaaa taattaattt tggaaggagg ttccatataa
     4681 atggcaaaga aatatagcag atctcagtac aaaaaagaga gaaatgcaaa taaagatact
     4741 agaccaaaag ctacattaag aaacgctaga gtttctacaa caaaagcagc ttttgtatta
     4801 gatgcgatca gaggaaaaga cgtagaaaca gcacttggaa ttttacttta caatccaaga
     4861 tatgcttcaa cattaattga aaaattatta aaatctgcaa tcgctaatgc agaaaacaat
     4921 aacggaatgg acccagaaaa cctttacatt gcagaatgtt atgcaaataa aggacctaca
     4981 atgaagagaa ttaaacctag agcacagggt agagcttaca gaatcgaaca tagaatgagc
     5041 cacattacag tagtgcttga tgaaagatag atagaaggag gcaaacatgg gacagaaagt
     5101 taatcctcat ggtctgagag tcggaattat taaagattgg gattctagat ggtatgcaga
     5161 agacgctttt gcagataacc ttgtagaaga cgataaaatc agaaaatatg taaagaaaag
     5221 attatacaat gctggaattt ctaagatcga gatcgaaaga gcttccgaca gagtaaaatt
     5281 aat
//
LOCUS       DAOMUE010000128         5281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4309_length_5281_cov_2.858018, whole genome shotgun sequence.
ACCESSION   DAOMUE010000128 DAOMUE010000000
VERSION     DAOMUE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4309_length_5281_cov_2.858018"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1976
                     /locus_tag="PLC71_06675"
     CDS             3..1976
                     /locus_tag="PLC71_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRM02360.1"
                     /translation="MICSILYKELGGAGSVLVVLLISVISLILVTERSLLSFLKKAVD
                     FFTVEGYEEDIEERLAKKKRKAKEVSKEDKETAPRKKRSMHIPDLRSGRPKPSQASPM
                     PPMEPEEKTKQTPIQKDFDDLIVQEETDKEPKEIFEPVQSNASKRSLVNKDKNTPSKP
                     VEILPQVRTKEGDYKFPPVSLLEKGKKTAGNNKEELRQTAQKLQKTLEDFGVHVTITN
                     ISCGPSVTQFELHPEQGVKVSKIVNLADDIKLNLAAADIRIEAPIPGKSAIGIEVPNK
                     TNQMVMFRELIENQEFAQARSKIAFAVGKNLAGQVIVSDIAKMPHLLIAGATGSGKSV
                     CINTLIMSILYKATPDEVKLIMIDPKVVELSAYQGIPHLLIPVVTDPKQASSALNWAV
                     MEMGERYKKFAEVNVRNLTGYNEKVEESIKNGMEGEDFKKLPQIVIIVDELADLMMVA
                     PGEVEDAIVRLSQLARAAGIHLVIATQRPSVNVITGLIKANVPSRIAFSVSSGVDSRT
                     IIDMNGAEKLLGKGDMLFYPSGYQKPIRVQGAFVSDEEVSKVVEFLKEENNAEDSYGA
                     DIQEKIQTAAVKAATSQERDEYFEKAAEFIIDKDKASIASLQRVFKIGFNRAARLMDQ
                     LCEAGIVGEEEGTKPRKVLMSEEQFEQYKEDYL"
     gene            1990..3663
                     /locus_tag="PLC71_06680"
     CDS             1990..3663
                     /locus_tag="PLC71_06680"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRM02361.1"
                     /translation="MKTDIEIAQEATLLPIKEVAANIGIKEDDLELYGKYKAKFDSHF
                     MDEVKNNEDGKLVLVTAINPTPAGEGKTTTSVGVSQALNKLGKKTICALREPSLGPCF
                     GIKGGAAGGGYAQVVPMEDLNLHFTGDFHAITSANNLLAAMLDNHIQQGNKLNIDTRD
                     VVWKRCIDMNDRALRNTVIGLGRRVDGVVREDHFVITVASEIMAILCLANDMADLKER
                     LGKIIVAYNMDREPVTAKDLNAVGAMAALLKDAIKPNMIQTLEHTPAFVHGGPFANIA
                     HGCNSVQATKLALKLADIAITEAGFGADLGAEKFMDIKCQMADLHPDAVVLVATVRAL
                     KYNGGVPKDNLAEENIDALSKGIVNLEKHIENLKLFGVPVVVTLNQFATDTEAELTFI
                     KNFCEERDCDFALSQVWEKGGEGGIELAKAILRTLDNKESNYKPLYTYDDTTIEEKIE
                     TIATKIYGADKVVYTAAAARQKKRLTELGYGNLPICMAKNQYSLSDDPKKLGRPEGFD
                     ITIREIYVNAGAGFLVALTGDVMTMPGLPKKPAAEGIDVNDDGVITGLF"
     gene            3715..4560
                     /gene="folD"
                     /locus_tag="PLC71_06685"
     CDS             3715..4560
                     /gene="folD"
                     /locus_tag="PLC71_06685"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRM02362.1"
                     /translation="MQIIDGKKISQEIKDEVKEEVAQLKAEGKDIALAVIQVGADPAS
                     SVYVRNKKRACEYVGIESVSYELPEETTQEELMAIVDKCNKDPKINGILVQLPLPKHL
                     DEDEVLLAIDPKKDVDGFHPVSVGNMVIGEKGFLPCTPAGVIQLLKRSGVEIDGKECV
                     VVGRSNIVGKPMAMLLLRENGTVTVCHSHTKDLKEVCKRADILVVAIGKPKFIDDSYV
                     KDGAVVIDVGIHRNENNKLCGDVDYDKVAPKTSAITPVPGGVGPMTIAMLMKNCVESK
                     KLFGE"
     gene            4566..>5281
                     /locus_tag="PLC71_06690"
     CDS             4566..>5281
                     /locus_tag="PLC71_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM02363.1"
                     /translation="MKILFISLGCDKNLVDSEVMLGLLTKNGYTLTDDETQADVIVIN
                     TCCFIHDAKEESINTILEMAQYKEQNLKALVVAGCLAERYKDEILKEIPEIDAVLGTT
                     SYDSIVEAVNTALEENTGEHFEHYESIDYLPDNSEVERVVTTGNHMAYLKIAEGCDKR
                     CSYCIIPKIRGRFRSVPMQELLNEAKRLASEGVKELVLVAQETTLYGKDLTGEKQLPL
                     LLHELCKIEGIEWIRLLYCYP"
BASE COUNT         1866 a          862 c         1224 g         1329 t
ORIGIN      
        1 cattgatctg ttcaatcctt tataaggaac tgggaggggc aggaagtgtt ttggttgttt
       61 tattgatctc tgtaatctca ttgatccttg taacagagag atccttactt tctttcctta
      121 agaaagcagt tgatttcttt acagtagaag gatatgaaga agatatagaa gaacgactag
      181 cgaagaagaa acgaaaagca aaagaggtgt ccaaagagga taaagaaaca gcaccgagaa
      241 agaaacgatc gatgcatatt ccggatttgc gatctggaag accgaaacca tcacaggcat
      301 ctccaatgcc tcctatggaa ccagaagaga aaacaaaaca aacaccgata cagaaggatt
      361 ttgatgatct gatcgttcaa gaagaaacag acaaagaacc aaaagaaata tttgaaccag
      421 tacagtcgaa tgcatcgaag agatctcttg tgaataagga taagaataca ccatcgaaac
      481 cagtggagat tcttccgcag gttcggacta aagaaggaga ttataagttt ccaccagtat
      541 ctttattgga gaaaggaaag aaaacagcgg gaaataacaa agaagaactt cgtcagactg
      601 cacaaaaact gcaaaaaaca ttggaagatt tcggagttca tgttacaatt accaatatta
      661 gctgtggacc atccgttaca cagtttgaat tacatccaga acaaggtgtg aaagtcagca
      721 agatcgtaaa tcttgcggat gatattaagt taaaccttgc agcagcagat attcgaattg
      781 aagcaccgat tccaggtaaa tcagcaattg gaatcgaagt tccgaataag acaaaccaga
      841 tggtaatgtt cagggaactg attgagaatc aggaatttgc acaggcaaga tcaaagattg
      901 catttgcagt aggaaagaac cttgctggac aggtgatcgt gtcagatatt gccaagatgc
      961 cgcatctttt gatcgcagga gcaacaggtt ctggtaagtc agtttgtatt aatacattga
     1021 tcatgagtat tttatacaag gcaacgccag atgaagtgaa actgatcatg atcgatccaa
     1081 aggtcgtaga attaagtgca tatcagggaa ttccacactt gctgattccg gtcgttacag
     1141 atccgaagca ggcatccagt gcgttaaact gggctgtgat ggagatggga gagcgatata
     1201 agaagtttgc agaagtgaat gtaagaaact taactggata taatgagaag gtcgaggaat
     1261 cgatcaagaa tggtatggaa ggggaagatt ttaagaaact tccgcagatc gttatcattg
     1321 tagatgagct tgctgacctt atgatggttg caccgggtga ggtcgaagat gcaattgtac
     1381 gtttatcaca gcttgcaagg gcagcaggaa ttcatctggt gatcgcaaca cagagaccat
     1441 ctgttaacgt cattacaggc ctgatcaaag cgaatgtacc ttcaagaatt gcattttcag
     1501 tatcatcagg agtcgattca agaacaatta tcgatatgaa tggtgcagaa aagttattag
     1561 gaaaaggtga tatgctgttc tatccatctg gatatcagaa accaatccgt gtacaaggtg
     1621 catttgtcag tgatgaagaa gtcagcaagg ttgtagaatt cttaaaggaa gaaaataatg
     1681 ccgaagacag ctatggtgca gacattcagg aaaagattca gacagctgcg gtcaaagcag
     1741 caacatcgca ggaaagggat gaatattttg aaaaagcagc tgagtttatt attgacaaag
     1801 acaaggcttc gatcgcaagt cttcaaagag tctttaagat tggatttaac cgtgcagcaa
     1861 gactaatgga tcagttatgc gaagcgggaa tcgtcggaga agaagaagga accaagccaa
     1921 ggaaagttct tatgagcgaa gaacaatttg aacaatacaa agaagattat ctatagaaag
     1981 gaaactatta tgaaaacaga tattgaaatc gcacaggaag ccacattatt accgatcaaa
     2041 gaagttgcag cgaatatcgg gatcaaagaa gacgaccttg aattatatgg aaaatacaag
     2101 gccaaatttg attctcattt catggatgaa gttaagaata acgaagatgg gaaacttgtt
     2161 cttgtgacag cgatcaaccc aactccagca ggtgaaggaa agacaacaac aagtgtcgga
     2221 gtatcccagg cgttaaacaa acttggaaag aaaacaatct gtgctcttcg tgagccatct
     2281 cttggaccat gctttggtat caaaggtgga gcagccggtg gtggatatgc acaggttgta
     2341 ccaatggaag atttaaacct tcattttaca ggggattttc atgcgatcac atctgcaaac
     2401 aacttattag cagcaatgct tgataatcac attcagcagg gaaacaaatt aaatatcgat
     2461 acaagagatg ttgtatggaa gagatgtatt gatatgaatg accgtgcatt aagaaataca
     2521 gtcatcggac ttggacgtcg tgtggatggt gttgtccgtg aggatcattt tgtcatcaca
     2581 gtagcatctg agatcatggc aattctttgc cttgcaaatg atatggcaga tttaaaagaa
     2641 cgtttaggaa agatcatcgt tgcatacaac atggaccgtg aaccagtcac agccaaagac
     2701 ttgaatgcag ttggagctat ggcggcatta ttaaaagatg ctattaaacc aaatatgatc
     2761 cagacattag agcatacacc agcattcgta catggtggac catttgccaa tatcgcacat
     2821 ggatgtaata gcgtacaggc tacaaaatta gcgttaaaat tagcagatat cgcaattaca
     2881 gaagcaggat tcggtgctga ccttggtgca gagaaattta tggatattaa gtgtcagatg
     2941 gctgatcttc atccagatgc agttgtatta gtagcaactg tacgtgcttt aaaatacaat
     3001 ggtggagttc caaaagacaa tctggcagaa gaaaatatcg atgcgttaag caaaggtatc
     3061 gtaaatcttg aaaaacatat cgagaactta aaattgtttg gagttcctgt tgttgtaaca
     3121 ttaaaccagt ttgcaacaga tacagaggca gaacttacat ttatcaagaa tttctgcgaa
     3181 gaaagagatt gtgattttgc attatctcag gtatgggaaa aaggtggaga aggtggaatc
     3241 gaacttgcca aagcaatcct tcgtacatta gataataaag aaagcaatta caaaccatta
     3301 tatacatatg atgatacaac gatagaagaa aagatcgaaa cgatcgcaac taagatttat
     3361 ggagcagata aagttgtata cacagcagct gcagcacgtc agaagaagag actgactgaa
     3421 cttgggtatg gaaatcttcc aatctgtatg gcgaagaacc agtattcttt atctgatgat
     3481 cctaagaagc ttggacgccc agaaggattt gatattacga tccgtgagat ctatgtcaat
     3541 gcaggtgcag gattcttagt agccttgaca ggtgatgtca tgacaatgcc tggacttcca
     3601 aagaaaccag cagcagaagg aatcgatgta aatgacgatg gagttatcac aggattattc
     3661 taatcaacaa aaataaagcc aatatatact taaaagaaat gacgaggaaa tacaatgcag
     3721 attattgatg gaaagaagat ttctcaggaa atcaaagatg aagtaaaaga agaagtagcg
     3781 cagctgaaag cagagggaaa agatattgcg cttgctgtta ttcaggtagg agcagatcct
     3841 gcatcaagtg tttacgtacg caataaaaaa cgtgcctgcg agtatgtagg aatcgagtct
     3901 gtaagctatg agcttccaga agagacaaca caggaagaat taatggcgat cgtggataaa
     3961 tgcaataagg atcctaagat caacggaatc ctagtacagc ttccacttcc aaaacatttg
     4021 gatgaagatg aagtattatt agcaatcgat cctaagaaag atgttgacgg attccatcca
     4081 gtcagtgttg gaaatatggt gatcggagaa aaaggattct taccatgcac accagccgga
     4141 gtgatccagt tattaaaacg ttctggtgtc gaaattgatg ggaaagaatg tgtggtagtc
     4201 ggaagaagta acattgtagg aaaacctatg gcaatgttat tattacgtga gaacggaaca
     4261 gtgactgtct gccattctca tacaaaagac ttaaaagaag tctgcaaacg tgcagatatc
     4321 ttggttgtag caattggtaa accaaaattt attgatgatt cttatgtaaa agatggagca
     4381 gttgtcattg atgttggaat ccacagaaat gaaaataaca aactttgtgg tgacgtagat
     4441 tatgacaaag ttgcaccaaa gacatctgcg atcacaccag ttccaggtgg agttggacca
     4501 atgacgatcg caatgttaat gaaaaactgt gtagaatcta agaaattgtt tggagagtaa
     4561 gagaaatgaa gatattattt atttcgcttg gatgtgataa aaacttagta gacagtgaag
     4621 ttatgcttgg acttttgacc aagaatggct acactttgac cgacgatgag acacaggcag
     4681 atgttattgt aattaataca tgctgtttca tccatgatgc caaagaagaa agtatcaata
     4741 cgatcttaga gatggcacag tacaaagaac agaatctgaa ggcattggtt gttgctggat
     4801 gcctcgcaga acgttataaa gacgagatct taaaagagat tccagagatt gatgcagttc
     4861 ttggaacaac aagttatgat tctattgtag aagcagtgaa taccgcatta gaagaaaata
     4921 caggggaaca ttttgaacat tacgaaagta ttgattatct tccagataat tctgaagtag
     4981 aacgtgttgt aacaacagga aatcacatgg catatttaaa aattgctgaa ggttgtgata
     5041 aacgttgcag ctattgtatc attccaaaga tccgcggacg tttcagaagt gttccaatgc
     5101 aagaactgtt gaatgaggca aagcgtttag catctgaagg agtgaaagaa ttagtactgg
     5161 tagctcagga aacaacactg tatggcaaag acctgacagg agaaaaacag cttccgttat
     5221 tacttcatga actttgcaag atcgaaggga ttgaatggat ccgcctgtta tactgctacc
     5281 c
//
LOCUS       DAOMUE010000129         5235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4363_length_5235_cov_3.458494, whole genome shotgun sequence.
ACCESSION   DAOMUE010000129 DAOMUE010000000
VERSION     DAOMUE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4363_length_5235_cov_3.458494"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /gene="bioB"
                     /locus_tag="PLC71_06695"
     CDS             complement(<1..884)
                     /gene="bioB"
                     /locus_tag="PLC71_06695"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRM02364.1"
                     /translation="MNSEQNLTREEAIAILNTPDEQLNELIERAGALRKKYKGNHVSI
                     HLLTNARSGNCSQNCAYCAQSCRSHADIEKYKWVDDEKLYGDNTFVHDHKLSRHCIGL
                     SGMKFSDAEIEELASKIRKMKEEGTHLCCSIGFLTKKQALMLKEAGLDRINHNLNTSR
                     SNYPNICTTHTYEQRVNNIHMLQDLGFEICSGGIIGMGESKEDVVDMLLDLKEINPEA
                     LPINFLIPIKGTPLQDRDTSNLTPEYCLKVLCLARLLVPTSDIRCAAGREIYFKGREK
                     ELLTVVNSIFASGYLTADG"
     gene            1088..1570
                     /locus_tag="PLC71_06700"
     CDS             1088..1570
                     /locus_tag="PLC71_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRM02365.1"
                     /translation="MQDINDKINNMHMGKLIHMLSHEMKRNQPIDKVIKADLTIMQKH
                     ILKFILLETMHKDLYQKDIEEEFQIRKSTVTGYVQLMEKNGYLTRESVKGDARKKRLV
                     PTEKAEGLRQAILEDIKLSEKRMEKGIPEEDVITCKHVLYQVLQNLIEKHKEEGKKDE
                     "
     gene            1563..3296
                     /locus_tag="PLC71_06705"
     CDS             1563..3296
                     /locus_tag="PLC71_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM02366.1"
                     /translation="MNKTLLKSVREYKKQSILAPILVILEVLMEVLIPMEMANIIDIG
                     MTSGDLHYIIQRGVILVVMAMLSLFFGISAGNMAAVAGAGYAKNLRHDIFYKVQEFSF
                     KNIDHFATSGLVTRMTTDITNIQMAYMMSIRLLARAPIMIVLSWVMTLKYSVKVALLF
                     LIVIPLLGGTLIFIAKKAHPHFIKVFDEYDILNNSVQENVNASRVVKAFVREDYEIDK
                     FHGISKYVYTLFTKAEKIVAWNSPVMQFTMYVVILLLVAIGGTGIIHGTMGTGELTSI
                     IVYALQIIGSLMMVTFVFVMIMIAEASTDRITEVLEEVPEMQDKADAVTEVKDGEIIF
                     DHVNFSYAGEGGNLSLKDVDLHIRSGQTIGIIGGTGSAKSTLVQLIPRLYDVTEGCVK
                     VGGIDVRDYNLEALRDQVSMVLQKNVLFTGTIYDNIRWGDENASDEEVQRMCKLAQAD
                     GFVNEFPAGYNTQIVQGGNNVSGGQKQRLCIARALLKKPKILILDDSTSAVDTKTDAL
                     IRKAFREEIPNTTKIIIAQRVSSIEDADQIIVLDDGKIMGVGTSEELLKTNDIYREVY
                     ESQVKGGEDDE"
     gene            3289..5178
                     /locus_tag="PLC71_06710"
     CDS             3289..5178
                     /locus_tag="PLC71_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM02367.1"
                     /translation="MSKRPEKTINKNTAKTAKRLLKYVTDTYKLQFILVFVCIFISAA
                     ASVSVSLSLKFMLDDFILPLVGQSNPDFTNFYHAMIVLGCIFAAGVIATFIYTRMMVF
                     IGQGVLKRVRDDMFEHMQTLPIRYFDQNTNGSIMSLYTNDTDTLRQMISQAIPQALMS
                     FFTIIVTFISMLVLSPILTILAVVVIGIMITVTKAIGGNSGKYFIRQQKSLADVTGFI
                     EERMNGQRVVKVFNHERKSEEEFDKLNESLFESAANANKYANIMGPVVGNIGNLQFVL
                     TAVLGGVLAIEGIGGITLGVMASYLQFTKSFTQPFMQVAQQFNSIVMALAGAERIFAL
                     IDEEPETDEGYVRLVNAKKDENGNIIECEERTGMWAWKHPHSADGSVTYTELTGDVRF
                     EDVTFGYNEDKTILHDISLFAKPGQKLAFVGSTGAGKTTITNLINRFYDIQDGKIRYD
                     GINIKKIKKDDLRRSLGIVLQDTHLFTGTIKDNIRYGNLGATDEQIYEAAKLAHADQF
                     IKMLPDGYDTVISGDGEGLSQGQRQLLSIARAAVANPPVLILDEATSSIDTRTESIVQ
                     RGMDNLMKGRTVFVIAHRLSTIRNSDAIIVLEHGKIIERGDHEDLIKNKGTYYQLYTG
                     KLELS"
BASE COUNT         1786 a          887 c         1091 g         1471 t
ORIGIN      
        1 ccatctgcag tcagatatcc tgaagcaaag atagaattta caacggtcaa taattccttt
       61 tcccttcctt taaaataaat ttcacgtcca gcggcacagc ggatatcaga tgttggaacc
      121 agtaatcttg ccagacataa tactttcagg cagtattctg gtgtcaaatt tgaagtatct
      181 ctatcctgca gaggtgttcc tttgatcgga attaagaagt tgatcggaag tgcttctgga
      241 ttgatttctt tcagatccaa taacatatca acaacatctt ccttactctc gcccatacca
      301 atgataccac cgctgcagat ttcaaatcca aggtcctgta acatatgaat gttgtttaca
      361 cgctgttcgt atgtatgtgt tgtgcagata tttggataat tggaacggct tgtatttaag
      421 ttatggttga tacggtctaa tccagcttct tttaacatta aggcctgttt ctttgttaaa
      481 aatccgatag agcagcataa atgtgtaccc tcttctttca tcttacgaat tttagaagca
      541 agttcttcaa tctcagcatc gctgaatttc ataccgctta atccgataca atgacgggaa
      601 agtttatgat catggacaaa tgtgttatct ccatataatt tttcatcatc gacccattta
      661 tatttttcaa tatctgcatg ggaacgacag gattgtgcgc agtaagcaca gttctgggag
      721 cagtttccgc ttctggcgtt tgttaaaaga tgaatgctta catggttccc tttatatttt
      781 ttacgaagtg ctccggcacg ttcaatcagt tcatttaatt gttcatcagg tgtatttaag
      841 attgcgatgg cttcttcacg ggttaaattt tgttctgaat tcatataata tataccttcc
      901 tttatttaaa ttcgaaatat cttattaatc tgaactttag catggaaaaa aacaattgtc
      961 aacatagtga gtaaatttgg ttaacgataa tagagataat aaaataacga ttgacattca
     1021 tttttattct ctgtataata ctaaaaagtt aggaagctaa ccattttgaa aagaaaggga
     1081 tgagtgaatg caggatataa atgataagat caataacatg cacatgggaa aactgatcca
     1141 tatgttatca catgagatga agaggaatca gccaattgat aaagtgatca aggctgatct
     1201 taccatcatg cagaaacata ttttaaagtt tattttatta gaaacaatgc ataaagatct
     1261 ttatcaaaaa gacattgaag aagaatttca gatccgcaaa tcaactgtga caggatatgt
     1321 acagttgatg gagaagaatg gatatctgac aagagaaagt gtcaaaggag atgctagaaa
     1381 gaagcggctg gtaccaacag agaaagccga aggattaaga caggcaatct tggaagatat
     1441 taagcttagt gaaaaaagaa tggaaaaagg cattcctgag gaagatgtca taacatgcaa
     1501 acatgtttta tatcaggtat tacaaaatct tatagaaaaa cacaaggagg aaggaaagaa
     1561 agatgaataa gaccctatta aaatcagtcc gagaatacaa aaaacaatcc atacttgctc
     1621 caatcctggt aattcttgag gtactgatgg aagttttgat tccgatggag atggcaaata
     1681 tcattgatat cggaatgaca agcggagatt tacattatat tatccagcga ggagtgatcc
     1741 tggttgttat ggcaatgtta tccctgtttt tcgggatttc agctggaaat atggcagccg
     1801 ttgcaggtgc cggatatgca aagaacttaa gacacgatat attctacaaa gtgcaggagt
     1861 tctcatttaa gaatatcgac cactttgcaa catcaggact tgtcacacgt atgacaacag
     1921 atattacaaa tatccagatg gcctatatga tgagtatccg actacttgcc agagcaccga
     1981 tcatgatcgt tttatcatgg gtaatgacat taaagtacag tgtgaaagta gcgttactat
     2041 ttttgatcgt gattccatta ctaggaggaa cattgatctt tattgcgaaa aaagcacatc
     2101 cacactttat caaagtattt gatgaatatg atatcttaaa taattctgta caggaaaatg
     2161 taaacgcatc ccgtgtggta aaagcatttg tacgtgaaga ttatgaaatc gataaattcc
     2221 acggaatttc caagtatgta tatacactgt ttacaaaagc agaaaagatt gttgcatgga
     2281 actcaccagt tatgcagttt acaatgtatg ttgttatact tctgcttgta gcgattggtg
     2341 gaacagggat catccatgga acaatgggaa ctggagaatt aacaagtatc atcgtatacg
     2401 cattacagat cattggttct ctgatgatgg taaccttcgt attcgtaatg atcatgatcg
     2461 cagaagcatc tacagaccgt attacagaag ttttggaaga agtaccagaa atgcaggata
     2521 aagcagatgc agtaacagaa gtgaaagacg gagagatcat ctttgatcat gtaaacttca
     2581 gttatgcagg agaaggtgga aatctttcct taaaagatgt tgatcttcat atcagatccg
     2641 gacagacgat tggaatcatc ggtggaacag gtagtgcaaa atctacatta gtacagttga
     2701 tcccaagact ttacgatgtt acagaaggat gtgtcaaagt cggcggcatt gatgtccgtg
     2761 attacaactt agaagcatta agagaccagg tatccatggt actccagaag aatgtattat
     2821 ttacaggaac aatttacgat aatatccgtt ggggagatga aaatgcatcc gatgaagaag
     2881 ttcagagaat gtgtaaatta gcacaggcag acgggtttgt aaatgaattt ccagctggat
     2941 acaatacaca gatcgtacag ggtggaaaca atgtttctgg tggacagaaa cagagattgt
     3001 gtatcgcaag agcgttatta aagaaaccaa agatcttgat cttagatgat tccacaagtg
     3061 cggttgatac aaagacagat gcattgatcc gtaaggcatt ccgtgaagaa attccgaata
     3121 caacaaagat catcatcgca cagagagtat cttccatcga agatgcagac cagatcatcg
     3181 tattagatga tggaaagatc atgggtgttg gaacatccga agaattatta aagacaaatg
     3241 atatttacag agaagtctat gaatcacagg tgaaaggggg agaagacgat gagtaaaaga
     3301 ccagagaaga cgatcaacaa aaatacggcg aagacagcaa aacgtctttt aaaatatgta
     3361 acagatactt ataaattaca gtttattctc gtgtttgtct gcattttcat cagtgcagca
     3421 gcttctgtat ctgtatcttt atcattgaaa tttatgctcg atgacttcat cctgcctttg
     3481 gttggacaga gcaatccaga tttcacaaat ttttaccacg caatgatcgt ccttggatgc
     3541 atctttgcag caggtgtcat tgcaacattt atttatacaa ggatgatggt atttatcgga
     3601 caaggagtcc taaaacgtgt cagagacgat atgtttgaac atatgcagac attaccgatc
     3661 cgttatttcg accagaatac aaatggttcg atcatgagtt tatacacaaa tgatacggac
     3721 acattaagac agatgatcag ccaggcaatt cctcaggcat tgatgtcatt tttcacgatc
     3781 attgtaacat ttatttctat gttagtatta agtccgattt taacgattct tgcagttgtt
     3841 gttatcggaa tcatgatcac ggtgacaaaa gcgattggtg gaaacagcgg aaaatacttt
     3901 atccgccagc aaaaatcatt agccgatgtg acaggattta tcgaagagag aatgaatgga
     3961 cagagagttg tcaaagtctt taaccacgaa cgtaaatcag aagaagaatt tgacaagtta
     4021 aacgaatctc tgtttgaaag tgctgcaaat gccaacaaat atgcaaatat catgggtcca
     4081 gttgtaggta atatcggaaa ccttcaattt gtattaacag cggtattagg tggtgtgtta
     4141 gctattgaag gtattggagg gatcacatta ggtgtcatgg catcatacct tcagtttaca
     4201 aagagtttca cacagccatt tatgcaggtc gcacagcagt ttaactctat tgtaatggca
     4261 cttgcaggtg cagaaaggat ctttgccctg atcgatgaag aacctgagac agatgaggga
     4321 tatgtaagat tagtaaatgc gaagaaagat gaaaatggaa acattatcga atgtgaagaa
     4381 agaacaggaa tgtgggcatg gaaacatcca cactctgcag atgggtctgt aacatataca
     4441 gaattaactg gagatgtaag atttgaagat gtaacgtttg gttacaacga agataaaacc
     4501 atccttcatg acattagtct gttcgcaaaa ccaggacaga agctagcatt tgtaggatcc
     4561 acaggagccg gaaaaacaac gatcacaaac ctgatcaacc gcttctatga cattcaggat
     4621 ggtaagattc gttacgacgg aatcaacatc aagaagatca aaaaagacga cttaagacgt
     4681 tccttaggaa ttgtattaca ggatacacac ctgtttacag gaacgatcaa agacaatatc
     4741 cgctacggaa atttaggggc aaccgacgaa cagatctacg aagcagccaa attagcacac
     4801 gcagaccagt tcatcaaaat gcttccagat ggttacgaca ctgtgatcag cggagacgga
     4861 gaaggattat ctcaaggaca gagacagtta ctatccattg ccagagcagc cgtagccaac
     4921 ccaccagtac tgatcttaga tgaagcaaca tcaagtattg atacaagaac agaaagcatt
     4981 gtacagcgag gaatggataa cctgatgaaa ggtagaactg tatttgtaat cgcccatcga
     5041 ctatctacga tccgaaatag tgatgcgatc attgtacttg aacatggtaa gatcattgaa
     5101 cgtggagatc atgaagatct tattaagaat aaaggaacct actatcaatt atatactggt
     5161 aagttggaat tatcttaaat ttgtgattgg ccaactgtag attttgagta tatgataaaa
     5221 aataaataaa cgatc
//
LOCUS       DAOMUE010000130         5230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4370_length_5230_cov_2.836522, whole genome shotgun sequence.
ACCESSION   DAOMUE010000130 DAOMUE010000000
VERSION     DAOMUE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4370_length_5230_cov_2.836522"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..2658)
                     /locus_tag="PLC71_06715"
     CDS             complement(46..2658)
                     /locus_tag="PLC71_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRM02368.1"
                     /translation="MKKTYEFENLGCAHCAGKMEEKIGKLPDVESVNLSFPMKKMYLE
                     TSREDLLPEIQKICTDIEPDVIVTEVTKKSSKRRKHVEEDSHESHEHHHHHDEHCDCG
                     CDHNHNEHEHLEHHHHHDDHCDCGCEHDHEEHEHHKHHHHHDDHCDCGCCDDDDDDDE
                     SIAAAANREGNGQATVFIVEKLGCAHCAGKMEEQISHLPGVEAVNLTFATKQLRVWSD
                     DAKTLLPQIQDICTSIEPDVKVVVRENTVRAQKEAKKAKENSEDNREFWELGAGIVLF
                     IAGKALESSKPVYSMVCFILAYLILGIKIVWTALKNISKGQVFDENFLMSIATIGAFG
                     VGEFAEAVGVMMFYRIGELFEEKAVERSRSQIMDVIDMRPEVVNLVDDHGDVTVIDAE
                     EAEIGDILLVRPGDRIPLDGIITDGETMIDTSPVTGEPIPVSASVGTDVTSGCLNTSG
                     VIKIKVEKVLEESMVTRIMDSVENAAASKPKMDRFITRFSRVYTPFVVLLALATAIIP
                     SLVTGNWTHWIYTALTFLVISCPCALVLSVPLAFFSGIGAGSKIGILFKGGAALETLK
                     DITSVVMDKTGTITKGNFKVQDVIPLGNATRHEILSLAASCEESSTHPIGKSIMEAAK
                     DENVTYKTPENAKEIAGHGSVITIDNSEILAGNKKLMAQYHVTGDYPETTSYGTEVFL
                     AKDGVLIGAIVIADTLKDDAKSAIASLKAQNLHTVMLTGDSETTANAIAKETGIDEVY
                     SRLLPDEKLAKLQEQRSKHGAVMFVGDGINDAPVLAGADCGAAMGSGADAAIEAADVV
                     FMTSNVEAIPQAISIGKKACKIAWQNVVFALAIKALVMILGLLGFANMWMAVFADSGV
                     AMLCVINSIRAMKL"
     gene            complement(2642..3034)
                     /locus_tag="PLC71_06720"
     CDS             complement(2642..3034)
                     /locus_tag="PLC71_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRM02369.1"
                     /translation="MDKTEKYNIASIDIHGHGCDVTELKHVLPPEEFAIETASLFGLL
                     SDSTRLRILYLLYHREVCVRNIAEAIEMSPPAVSHHLRSLKQLGVITSRRIGKEVHYT
                     IADTADGELVSDLLKTVFERRGIYEEDL"
     gene            3322..4608
                     /locus_tag="PLC71_06725"
     CDS             3322..4608
                     /locus_tag="PLC71_06725"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRM02370.1"
                     /translation="MEELKNIKVALLGSGTVGTGVYKLIERQREELPHKVGAGLTVSK
                     VLVRDKNKKREGIDPSVLTDQWEDIITDDEIQIVVEVMGGIEPARTYITEALQAGKNV
                     VTANKDLMAVHGGELLDIASHNGCDLLFEASVAGGIPIIRPLKQCLAGNYISEAMGIV
                     NGTTNFILTKMTHEGMEFDEALALATELGYAEADPTADIEGYDAGRKMAIMASIAFNS
                     RVTFDDVYTEGITNITAKDIKYAEAMGCTIKLLGVAKNTESGIEVRVHPMLIPSDHPL
                     ATVNDSFNAVFVHGDAVDDAMFYGRGAGEFPTASAVMGDIIDVARNIQFYCTGRIHCT
                     CYKDLPIKKITEIESKYFMRLLVDDKPGVLARIADTLGKNQVSIAQVIQKNKVNDMAE
                     LVVITDLVLEQNFADALVEIKGMEHTREISTVIRVY"
     gene            4926..5063
                     /locus_tag="PLC71_06730"
     CDS             4926..5063
                     /locus_tag="PLC71_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02371.1"
                     /translation="MEDYYSSKFQFHVIELSKIETTKGNHYMEKARDEMIKMSRDESE
                     R"
BASE COUNT         1667 a         1006 c         1024 g         1533 t
ORIGIN      
        1 ctttttataa tattccacca ataatttccg tcttcaaacg acaaatcaca atttcattgc
       61 acgtatagag tttattacgc atagcattgc gactccgcta tctgcgaaca cagccatcca
      121 catatttgcg aatccaagca gtccaaggat cattactaat gccttgatcg ccaatgcaaa
      181 tacaacattc tgccaagcga tcttacaagc ttttttgccg atagagattg cctgtggaat
      241 tgcttcaacg tttgaggtca tgaatacaac atctgctgct tcgattgcag catcagcacc
      301 gcttcccatg gcagcaccgc aatcagcacc agctaataca ggggcatcgt tgataccgtc
      361 accaacaaac ataacagctc catgtttgct tctctgttcc tgaagtttgg caagtttttc
      421 atcaggaaga agtcttgagt aaacttcgtc aattccagtt tcttttgcaa tcgcatttgc
      481 agttgtttcg gaatctccag ttaacataac cgtgtgaaga ttctgtgctt ttaaggaagc
      541 aatggcggat ttcgcatcgt cttttaatgt atctgcaata acgattgcac caattaacac
      601 accatctttt gcaagaaaaa cttcagttcc ataagaagtt gtttctggat agtctccagt
      661 tacatgatac tgagccatca attttttatt gccagctaag atttctgagt tatcaattgt
      721 gataacagaa ccatgtccag cgatttcttt tgcgttttca ggtgttttat aagttacatt
      781 ttcatctttg gcagcttcca tgatactctt tccgattgga tgagtagaag attcttcgca
      841 ggaagcagca agtgataata tttcatgtct ggttgcattt ccgagaggga tcacatcctg
      901 aactttgaag tttccttttg tgatcgttcc agtcttatcc atgacaacag aagtaatatc
      961 ttttaatgtt tcaagtgcag ccccgccttt aaataagata ccaatcttag atccagctcc
     1021 gattccagag aagaatgcaa gaggtacact taatacaagg gcacaaggac agctgattac
     1081 aaggaaagtt aaagctgtgt atatccagtg tgtccagttt cctgtcacta aagaaggaat
     1141 gatcgcagtt gcaagggcaa gaagtacgac aaatggagta tatacacgag aaaaacgtgt
     1201 gataaaacga tccatctttg gtttgcttgc ggctgcattt tctacggaat ccatgattct
     1261 tgtgaccata gattcttcca gtactttttc taccttgatc ttaataacac cagaagtatt
     1321 taaacatcca gaagttacat ctgttccgac agaggcagaa acaggaatag gttctccggt
     1381 aacaggagaa gtatcgatca tcgtttcccc atctgtgatg ataccatcta atggaatacg
     1441 gtcaccagga cgtactaata agatatctcc gatttcagct tcttcggcat caattacagt
     1501 gacatctcca tgatcatcaa caaggttgac aacttcaggt cgcatatcaa tgacatccat
     1561 gatctgggaa cggcttcgtt ccacagcttt ttcttcaaat aattcaccaa tacgataaaa
     1621 catcataaca ccgaccgctt ccgcaaattc tccgacgccg aaagcaccga tcgttgcgat
     1681 actcattaag aagttttcat caaaaacctg acctttagag atgtttttaa gagcggtcca
     1741 tacgatctta attcctaaga ttaaatatgc aaggataaaa cataccatac tgtatacagg
     1801 cttggaagat tccaatgctt ttccggcaat aaataaaacg ataccagcac caagttccca
     1861 gaattctctg ttatcttcag aattctcttt ggcttttttc gcttcttttt gtgcacggac
     1921 agtattttcg cggacaacga ctttgacatc tggttcgatg gaagtacaaa tatcttgaat
     1981 ctgaggcaat aatgttttgg catcatcaga ccatacacgt aactgctttg ttgcaaatgt
     2041 cagattgacg gcttcaacac cgggaagatg agaaatctgt tcttccattt tacctgcaca
     2101 atgggcacag cctaattttt cgacaataaa tacggttgcc tgaccatttc cttcacgatt
     2161 tgcagcagct gcaattgatt catcatcgtc atcgtcatca tcacagcagc cacaatcgca
     2221 gtggtcatca tgatggtgat gatgtttgtg gtgttcatgt tcttcgtgat cgtgttcaca
     2281 cccgcagtcg cagtggtcat catgatggtg atgatgttcg aggtgctcat gttcattatg
     2341 gttgtgatca catccacagt cacagtgttc atcgtgatgg tgatgatgtt catgactttc
     2401 atgagaatct tcttccacat gttttctccg tttagatgat tttttcgtaa cttccgtcac
     2461 aataacatcc ggttcaatat ccgtacaaat cttttgaatc tcaggaagca gatcttcacg
     2521 agaagtttca agatacattt ttttcatagg aaatgaaaga tttacacttt caacatcagg
     2581 aagtttcccg attttttctt ccattttacc tgcacaatga gcacagccaa gattctcaaa
     2641 ctcataagtc ttcttcataa atacccctcc tttcaaatac agtctttaac agatcagata
     2701 caagctctcc gtcagcagtg tctgcgatcg tataatgaac ttctttccca attcgtctgc
     2761 ttgtgatcac acctaattgc ttcaaagagc gaagatgatg agacactgct ggtgggctca
     2821 tctcaatcgc ttctgcgata tttctaacac agacttcacg gtggtataaa agatacaaaa
     2881 tacgaagtct ggtactgtca cttaacagac caaataaact ggcagtttca atggcaaatt
     2941 cttctggtgg aagaacatgt tttaattctg taacatcaca tccatgacca tgaatatcaa
     3001 tggaagcaat gttatatttc tccgttttat ccatagaaat ctccttttta taagattaaa
     3061 tgattgctta attgtttatg ttttaaatat aatactttta aatattgatg tcaacagaaa
     3121 tgagaaaaat agttaataga agtattgttt ggtgtaatat tggaactata cataatagtt
     3181 tttcctaact agaaattaaa aaaatatagg ataatcaaaa aaaatatgat atactaaaaa
     3241 ctacttatac gaatttatta gatcaaaaaa tcacatttcg ataaaagcaa agataaacaa
     3301 aaacagaaaa ggaagtgtat catggaagag ttaaagaaca tcaaagtagc cctccttggt
     3361 tctggaactg tcggaacagg agtatacaaa ctgatcgaac gccagagaga agaattacct
     3421 cacaaagttg gtgcaggtct gactgtcagc aaagttctag ttagagacaa gaacaagaag
     3481 agagaaggaa ttgacccttc tgtattaact gaccagtggg aagacattat cacagatgat
     3541 gagattcaga tcgtagtgga agttatgggg ggaatcgaac cagcccgtac ttatattaca
     3601 gaagctcttc aggcaggtaa gaatgttgtc acagcgaaca aagacttaat ggcagtgcat
     3661 ggtggtgaat tattagatat cgcttcccat aatggatgtg acttattatt tgaagcatct
     3721 gttgcaggtg gaattccaat tatccgtcca ttaaaacagt gtctggcagg aaactatatt
     3781 tcagaagcaa tggggattgt caatggaaca acaaacttca ttctgacaaa gatgacacac
     3841 gaaggaatgg aatttgatga agctttggca ctcgcaacag aattaggata tgcagaagca
     3901 gatccaacag ctgatatcga aggatatgat gcaggacgta agatggcgat catggcatcc
     3961 attgcgttta actctagagt cacatttgat gatgtataca cagaaggaat cacaaatatt
     4021 acagcaaaag atatcaaata tgcagaagca atgggatgta cgatcaaatt gttaggggta
     4081 gcaaagaaca cagaatctgg aattgaagta cgtgttcatc caatgctgat tccaagtgat
     4141 catccattgg caactgtcaa tgattcattt aacgcagttt ttgtacatgg agatgcagtt
     4201 gacgatgcaa tgttctatgg aagaggagca ggagaattcc caacagcaag tgcagttatg
     4261 ggagatatca ttgatgtcgc acgtaatatt cagttctatt gcacaggaag aatccattgt
     4321 acatgctaca aagaccttcc aatcaagaag atcacagaga ttgaaagcaa atatttcatg
     4381 agattgttag ttgatgacaa accaggagtt cttgctcgaa tcgcagatac attaggaaag
     4441 aatcaagtaa gtatcgcaca ggtgattcag aagaacaagg ttaatgatat ggcagaatta
     4501 gttgttatca cggatcttgt actggagcag aactttgcag atgctttggt tgagattaag
     4561 ggaatggaac atactcgtga gatttctaca gtaatcagag tgtattaaat tttaattgta
     4621 gttggccaac tgtagatttt gagtatatga taaaaaataa ataaacgatc ggaagagcag
     4681 ctagagaatt ttcctcacgt tttttacaga tcgctgatcc gccggctttc cttgcgtaac
     4741 tcgccttcaa gggtattgaa aacaggactc tttgaggctc aaacaacgca ccaagccggc
     4801 tgcgaatctt aaaaaaacgt ggcaaaattc taatgctgct cttccgatcg tttatttatt
     4861 ttttatcata taccagaagt ctagagtgtg gacaatttaa gattgaaaaa ttcaagtggg
     4921 cgtgtatgga ggactactat agtagtaaat ttcaattcca tgtgatagaa ttaagtaaaa
     4981 tagaaacaac aaaaggaaat cattacatgg aaaaggcgag ggatgagatg attaagatga
     5041 gcagggatga aagtgaaaga taatcttctg ttagcgaata caaaaatgat tagccaatac
     5101 cgttaaacat acacacagtg tagtggtttg aataacgtaa attatttgaa tattataaaa
     5161 agaaatcatg tgtcagcaac cgtacgaaca cgtctgcact tagcgagtgg atggtcgaat
     5221 gaccattcag
//
LOCUS       DAOMUE010000131         5143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4467_length_5143_cov_2.630700, whole genome shotgun sequence.
ACCESSION   DAOMUE010000131 DAOMUE010000000
VERSION     DAOMUE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4467_length_5143_cov_2.630700"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..487)
                     /locus_tag="PLC71_06735"
     CDS             complement(23..487)
                     /locus_tag="PLC71_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRM02372.1"
                     /translation="MKRVILRVMMLVAMASAAVTMSVHTSSKEVKASEKGDAVVEYAK
                     NFVGNRYRYGGSSLTNGTDCSGFTRGVYKKFGKRLPHSSSAQRRYGKKVKGGIKNAKA
                     GDLICYSGHVAIYMGNNKIVHASNSAPYPRGGIKISNNARYRRIVTVRRIVK"
     gene            complement(590..2674)
                     /locus_tag="PLC71_06740"
     CDS             complement(590..2674)
                     /locus_tag="PLC71_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRM02373.1"
                     /translation="MCGYKKIKIEYIMMAVAFASVVWSIFAGFRISRFQWLFVMGSVI
                     WFLGMCRLLDQNKRNIVVMVVICIIYCMLAHRQLINGFQIINNKMAEALNQSMDLGFY
                     YYISVTLEHSRRDSVLAVLFFVLVAGIVLGILRCRPLTLFLTTGLMEMAVLMIAPYGI
                     SAAFFLFLGSWIVYFSIRKGKKGFAAGLYIMFLAAAVPLFFYDQTNVPTDTMIKRNIL
                     IQIREWTQGKGYLAVGGIGNGKLRSVGEVSPKGEKLFLVYAPENADLYLKGFVSGRYK
                     DGEWTKEKKNNLVYGGEMAQELPYLFPDLSMKDFAVYQKGYIQTPKDIAFSEKREIKI
                     HYQKMQESFLLIPYFSDASRINGNAAGDSVIERNTADTEYTTSYYQIKNNKNLLDISQ
                     RFDVMSVLKNAGELEKNYVRSMQEYGTYVQQNYLEIPEEIKKTIKQLSCHVNKENSIL
                     YNIEEIQKFLKNNYQYTYRPGLTGQDKDPVNEFLTERKRGFCTQFASAAVFLFREAGI
                     PARYVEGYKIRADQWRLGKAQVTDYEAHAWTEIYIEHIGWIPVEVTGRDTGESVYKHV
                     EQEEKQRNAIVPNKKQFVTNVKKMFQMIPIVIILAVIFAFIKLLQKKRKWNQMTNKEK
                     VLFYEKQLEKYAENGVKSRNTRNSMTSEIIEKARYSNKDITRHELNLVKRHLDLLKRK
                     NGNVTKILTKLK"
     gene            complement(2674..3204)
                     /locus_tag="PLC71_06745"
     CDS             complement(2674..3204)
                     /locus_tag="PLC71_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRM02374.1"
                     /translation="MYKVKKEFNKQISFLVMPKRYEMGFMQEKIARRDLMEQGFEYDG
                     VRKYQEGDRISRVHWNLYAATGGLWVRKNEEEEEERVKIGLSLDDIEKDRISDYMAAF
                     YSISFFLKSQGVIQEIYYGEHKYLLCHIEQYEELFTDIFCGKYELSSDPMEHLYKIPL
                     CEKGQDLQKFLYDMEL"
     gene            complement(3349..3582)
                     /locus_tag="PLC71_06750"
     CDS             complement(3349..3582)
                     /locus_tag="PLC71_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02375.1"
                     /translation="MASLFVVLAYSVCRCQSEQKQMILTIEMDSLVLTEGDEAEIRLR
                     IHGITSKINSYTFRLEYEMTSKFRKQRMRKKKN"
     gene            complement(3657..4613)
                     /locus_tag="PLC71_06755"
     CDS             complement(3657..4613)
                     /locus_tag="PLC71_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRM02376.1"
                     /translation="MDNFFMYKKAVRAMDEINRVMEGKGHTVHQIMTAILAGGHILIE
                     DIPGVGKTTLALAFSKVLGLDYQRIQFTPDIMPTDIAGFSIYHQGSEEFEFKEGAAMT
                     NLLLADEINRTSPKTQSALLEAMEEGQITVDGITRDLPDPYIVIATQNPFGSIGTHSL
                     PESQLDRFMIRTSLGYPDMESEIKMLAQGSVDMTTSGIEEQISVEELRKAREEAARLF
                     VHEDILRYIVEIANETRRSRDLELGISPRGTIALLSMAKANAYLEGRDYVIPQDVKDV
                     FFETIEHRVIRSKEAKVQRKTKEEILKAILYKIPLPKGNQKG"
     gene            complement(4615..>5143)
                     /locus_tag="PLC71_06760"
     CDS             complement(4615..>5143)
                     /locus_tag="PLC71_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRM02377.1"
                     /translation="MTSAIMILCLAWTLSGVCGAEYLNIQGFVKAAVGGSTMAIAVMP
                     VIFFLISLGLAFATGTSWGTFGILIPISVTILSANYMMMSVCVGAILAGAVCGDHISP
                     ISDTTILSSAGAQCKHIDHVATQMPYALTVALCASVGYILSGITGNGYIGLAGAAISL
                     ALAMMIIVITAKRGR"
BASE COUNT         1443 a         1182 c          709 g         1809 t
ORIGIN      
        1 aaataccttc ttcttatcct ctttatttaa cgattcttcg tactgttacg attcttctgt
       61 atcttgcatt gtttgaaatc ttaattccac ctcttggata tggtgctgaa ttacttgcat
      121 gtacgatctt gttgtttccc atatagattg caacatgtcc gctatagcag attaaatctc
      181 ctgccttagc attcttgatc ccacctttta ctttcttacc atatcttctc tgtgcacttg
      241 aggaatgtgg aagacgcttt ccaaacttct tgtaaactcc tctcgtgaat cctgaacaat
      301 ctgttccgtt tgtaagactt gatccaccgt atctgtatct attccctaca aagttttttg
      361 catattcaac aactgcatct cctttttctg atgcttttac ttcttttgaa cttgtatgta
      421 cactcattgt aactgcggcc gatgccattg ctactaacat catcactctt aaaataactc
      481 gtttcatatt aactcctgat atattacaat tttaaattta tttcaaatat ttctttcaac
      541 agttgatatt gtacgataaa gagcaacaat tgtcaatagt tttttaagat tatttcagtt
      601 tagttaatat ttttgtaaca tttccgtttt ttctctttaa taaatcaaga tgtcgtttta
      661 caagatttag ttcatgtctg gtaatatcct tgttactgta ccttgctttc tctatgatct
      721 ctgacgtcat ggaatttctg gtattccttg attttacgcc attttcagca tatttttcta
      781 actgtttttc ataaaaaagt actttttctt tgtttgtcat ttgattccat tttcttttct
      841 tttgtaacaa ttttatgaac gcaaaaatca ctgctaatat tatgacgatt ggaatcattt
      901 gaaacatttt ctttacattt gttacgaatt gttttttatt tggtacaatc gcatttcgtt
      961 gtttttcttc ctgttccaca tgtttatata cactttctcc agtatctctt cccgttactt
     1021 ctaccggaat ccatccgata tgttcaatat aaatttctgt ccatgcatgt gcctcataat
     1081 ctgtcacctg tgcttttcca agtctccact gatctgcacg gatcttatat ccttctacat
     1141 atctggccgg aattcctgct tccctgaaca aaaaaactgc cgctgatgca aactgggtac
     1201 aaaaccctct ttttctctcc gttaaaaatt catttaccgg atccttatct tgccctgtta
     1261 aacctggtcg gtaagtatat tgataattat tttttaaaaa tttttgaatt tcttctatat
     1321 tatataagat actattttct ttatttacat ggcaactcag ctgctttatt gtcttcttta
     1381 tctcttctgg aatctccaga taattctgct gtacataagt tccatactcc tgcatagaac
     1441 gaacataatt cttttcaagc tctcccgcat ttttcaacac agacatcacg tcaaatctct
     1501 gactgatatc taacaggttc ttgttattct tgatctgata ataagaagtt gtatactccg
     1561 tatctgctgt attacgttct atcacactgt ctccagctgc atttccatta attctgctcg
     1621 catctgaaaa atatggtatt aataagaaac tctcctgcat cttctgataa tgaatcttta
     1681 tttctctctt ttcagaaaat gcaatatctt ttggtgtctg aatatatcct ttctgataaa
     1741 cagcaaaatc cttcatagag agatctggaa ataaatatgg cagttcctgt gccatttctc
     1801 caccgtagac caggttattt ttcttttcct ttgtccattc tccatcttta tatctgccac
     1861 taacaaatcc cttcagatac agatctgcat tttccggggc atacaccaga aataattttt
     1921 ctccttttgg agaaacttct cctacacttc gaagctttcc attcccgatt cctccgactg
     1981 caagatagcc ttttccctgt gtccattccc tgatctgtat cagaatattt cgttttatca
     2041 tcgtatctgt aggtacattc gtctgatcat aaaaaaacaa aggtactgcg gcagccagaa
     2101 acataatata caatcctgcc gcaaatcctt tttttccttt tcgtatacta aaataaacaa
     2161 tccatgaacc taaaaacaag aaaaacgctg ctgaaattcc atatggcgcg atcattaaaa
     2221 cggccatttc cataagaccg gttgtaagaa acaaagtcaa tggacgacat cttaatattc
     2281 caagcacaat tcctgccacc agcacaaaaa acaaaactgc cagtacgctg tcccttcgac
     2341 tatgttctaa cgtcactgaa atgtaataat aaaatccaag atccatactc tgattcaatg
     2401 cttctgccat cttattattg atgatctgaa atccattgat cagctgtctg tgtgcaagca
     2461 tgcagtatat gatacatatc acaaccatga ctacaatatt tctcttattc tgatcaagca
     2521 atctacacat tccgagaaac caaatgacac ttcccatcac aaaaagccac tgaaaccttg
     2581 aaatccgaaa tccagcaaat atactccata caacagatgc aaaagccact gccatcatga
     2641 tatattcaat ttttattttt ttatatccac acattataac tccatatcat ataaaaattt
     2701 ctgcagatct tgtccttttt cacaaagtgg aatcttataa agatgctcca tcggatccga
     2761 tgacaattca tattttccac aaaaaatatc cgtaaacaat tcttcatatt gttctatatg
     2821 gcaaagcaga tatttatgct ccccataata gatttcctgt ataactccct gcgatttcaa
     2881 aaagaaagaa atagaataaa aagctgccat ataatcagaa atacggtctt tttctatatc
     2941 atccaacgat aatccgatct tcacgcgttc ttcttcctct tcttcatttt ttctcaccca
     3001 aagtccacct gtcgcagcat agaggttcca atgaactctt gaaatccgat ctccttcctg
     3061 atatttcctg actccatcgt attcaaaacc ttgttccata agatctcttc ttgcgatctt
     3121 ttcctgcata aatcccattt catatcgttt tggcataaca agaaaagata tttgtttgtt
     3181 aaattctttt tttactttat acattcctgt aagatcttcc cacgaaactg aacaaatctg
     3241 aatatgataa ctgtcacatt ccgtgatcat ttcagacagt gtgatcgaat ctccttcttg
     3301 tggattccat acgatatttt tctgcctgga tttcttttac acaaagctct aatttttctt
     3361 ctttcgcatt ctttgttttc gaaacttgga tgtcatttca tattccaatc ggaatgtata
     3421 gctgtttatc tttgatgtaa ttccatgaat cctcaatcgt atctctgctt catcgccttc
     3481 tgtaagtact aatgaatcca tttctatggt taatatcatc tgtttttgtt ctgactgaca
     3541 cctgcagaca gaataagcca gcacaacaaa taaggatgcc ataaggcacc ctccaatgaa
     3601 atttccataa atgatccaat atataagcgc aaatattatg acaaaaaaat acatctttat
     3661 cctttctgat ttccttttgg aagtggtatc ttataaagaa tcgcttttaa aatctcttct
     3721 ttcgttttcc tctgaacctt cgcttccttg cttctgatca ccctatgctc gatagtttca
     3781 aagaaaacat ctttcacatc ctgtggtatt acataatcac gtccctcaag atatgcattt
     3841 gcttttgcca tcgataaaag tgcgatcgtt cctcttggac tgattccaag ttctaagtct
     3901 ctggatcttc ttgtctcatt cgcaatctcg acaatatatc ttaaaatatc ttcatgcaca
     3961 aataatcgtg cagcttcttc tcttgctttt cgcaattctt ccaccgagat ctgttcttcg
     4021 atcccagagg tcgtcatatc cacacttcct tgtgctaaca tcttgatctc actctccata
     4081 tccggatatc caagagatgt ccggatcata aatcggtcta actgtgattc tggaagcgag
     4141 tgtgttccaa tagaaccaaa aggattctgc gttgcaataa cgatatacgg atctggaaga
     4201 tctctcgtaa taccatctac cgtgatctgt ccttcttcca ttgcttctag aagtgccgac
     4261 tgtgtcttcg gtgaggttcg gttaatctca tccgccagca aaagattcgt catcgctgct
     4321 ccttctttga attcaaattc ttcactaccc tgatgataga tagaaaatcc tgcaatatcc
     4381 gttggcatga tatccggagt aaactgaatc ctttgataat ccagtcccaa tacctttgaa
     4441 aaggctaatg caagtgttgt ctttcctaca cctggaatat cctcgatcaa gatatgtcct
     4501 cctgcaagga tcgctgtcat gatctgatgc accgtatgac cttttccttc cataacacgg
     4561 ttgatctcat ccattgctct gactgctttt ttgtacataa agaaattatc catcttatct
     4621 tcctcgtttt gcagttataa caatgatcat catagcaagt gcaaggctta ttgctgctcc
     4681 tgcaagtcca atatatccat ttcctgtaat tcctgacaaa atatatccaa cacttgcaca
     4741 aagtgccact gttaatgcat atggcatctg tgttgccacg tggtctatgt gcttacactg
     4801 cgctcccgca gatgaaagga tcgtcgtatc agaaatcggt gaaatatgat ctccacaaac
     4861 agctcctgca agaatcgctc ctacacaaac tgacatcatc atataattag cacttaagat
     4921 cgtaacagaa attgggatca aaattccaaa tgttccccat gatgttcctg ttgcaaatgc
     4981 aagtcctaat gaaatcagga agaagataac cggcatcact gcgattgcca ttgtacttcc
     5041 accaacagct gctttaacaa atccctgaat atttaaatac tctgctccac aaacacctga
     5101 taatgtccat gcaagacaaa ggatcatgat cgctgatgtc att
//
LOCUS       DAOMUE010000132         5131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4487_length_5131_cov_2.794129, whole genome shotgun sequence.
ACCESSION   DAOMUE010000132 DAOMUE010000000
VERSION     DAOMUE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4487_length_5131_cov_2.794129"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..2210)
                     /locus_tag="PLC71_06765"
     CDS             complement(120..2210)
                     /locus_tag="PLC71_06765"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRM02378.1"
                     /translation="MTKQFSMELAGRTLRVDVGRVAAQANGAAFMHYGDTVVLSTATA
                     SEKPRDGIDFFPLSVEYEEKLYAVGKIPGGYLKREGKPSENAILTDRVIDRPMRPLFP
                     KDYRNDVALNNLVMSVDPDCSPELTAMLGSAIATAISDIPFDGPTASTMVGLIDGEFV
                     INPTSAQKEVSDLALTVASTREKVIMIEAGANEVPEDVMLEAIFKAHEVNQEIIKFID
                     TIVAECGKEKHDYEHHIVDPEMYDDMVAFITPEAMEEAVFTDDKQTREANIRAIEEKL
                     EERYAENEEWLAQIGEAVYAFQKKTVRKMILKDHKRPDGRDIKQIRPLHAEVDCLPRV
                     HGSALFQRGQTQVMTVTTLGSLSEAQRLDGIDVTETTKRYMHHYNFPSYSVGETRPSR
                     GPGRREIGHGALAERALVPVLPSEEEFPYAIRTVSEIMESNGSTSQGSICASSLSLMA
                     AGVPVKAPVAGISVGLVTGEGDDDYIILTDIQGLEDFFGDMDFKVAGTDKGITAIQMD
                     IKIHGLTEQIIREAIARTKEARTHILHDVMNPVIPAARDHVGEFAPKISQYTIDPEKI
                     SEVIGKQGKTINGIIDETGVKIDIDESGRIDILSEDQAMIDKAISIIKSIVEPLNVGD
                     IYEGEVVRIMNFGAFVQLSPNKDGMIHISKLSNERVEKVEDVVNIGDKVAVKVLEIDK
                     MGRINLKLEEKIED"
     gene            complement(2465..2731)
                     /gene="rpsO"
                     /locus_tag="PLC71_06770"
     CDS             complement(2465..2731)
                     /gene="rpsO"
                     /locus_tag="PLC71_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRM02379.1"
                     /translation="MISSEKKAEIVAKYGKDAKDTGSPEVQVAILTARINELQDHFNQ
                     HPKDHHSRRGLLKMVGKRRNLLAYLKSKDVERYRSLIEKLGLRK"
     gene            complement(2860..3672)
                     /gene="proC"
                     /locus_tag="PLC71_06775"
     CDS             complement(2860..3672)
                     /gene="proC"
                     /locus_tag="PLC71_06775"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRM02380.1"
                     /translation="MKLGFIGCGNMAQAMITGILTQKVVSPQELIVSNPREEKTAKLK
                     DEFGIITTTDNKEVAKQSDILVLSVKPQVYPAVIKEIRDEVSSEQIIVTIAAGVSMEA
                     AERQFGKEVKIVRVMPNTPALVGEGMSGLCCNEYVTEEEFDLVHKIFESFGKAEKITE
                     NLMDAVVGVSGSGPAYVYMFIEAMADAAVAQGLPRKQAYTFAAQTLLGSAKMVLETGQ
                     HPGELKDAVCSPAGTTIEAVNVLEKKGMRSAVIEAVTAAAKKNHQLGKKKEK"
     gene            complement(3697..4614)
                     /locus_tag="PLC71_06780"
     CDS             complement(3697..4614)
                     /locus_tag="PLC71_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRM02381.1"
                     /translation="MDETIEIKVTSEMAGKRLDVVLSEQCSDLTRSYINKLCKEERAA
                     LNGKTSKGNKKCKEGDVITLQVPEPTELEILPEEMNLDIVYEDQDVILINKPKGMVVH
                     PAAGHYSGTLVNGLMAHCKDDLSGINGVLRPGIVHRIDKDTTGILIVCKNDMAHQSIA
                     KQLYDHSITRKYHAIVYGNIKEEEGTVNAPIGRSLKDRKKMGIVMDGKHAVTHYKVLK
                     RLKKGFTYIECQLETGRTHQIRVHMASIKHPLLGDDVYGPKKSKYTLEGQCLHAKVLG
                     FVHPRTGEYMEFEVPLPEYFEKLLNKLDN"
     gene            complement(4592..5107)
                     /gene="lspA"
                     /locus_tag="PLC71_06785"
     CDS             complement(4592..5107)
                     /gene="lspA"
                     /locus_tag="PLC71_06785"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRM02382.1"
                     /translation="MKKQIRIYIYIIGLILIDQLSKVWALSALRGTEGIAVIPNVFEL
                     SYLENRGAAFGILQDHQIFFVLITVAAAVILTWIYRRIPQTKKYIPFRISYALIMAGA
                     FGNLIDRVFRGYVVDFFYFKWIDFPVFNVADIYVTVTMILLLILILFFYKEEDLDFLN
                     RKGKHGRDNRN"
BASE COUNT         1308 a         1183 c          847 g         1793 t
ORIGIN      
        1 gacgcattca cccacgccca tattaaaata tggacgtggt actctgctta gattttatag
       61 aacaaaaaag cagcttttca tacactttcg catacaaaaa gctgttttgt cattcttaat
      121 taatcttcaa tcttttcttc taatttcaga ttgattcttc ccatcttgtc gatctctaat
      181 actttgacag ctactttgtc tccgatattc acgacatctt ctactttctc aactctttca
      241 ttagaaagtt ttgaaatgtg gatcattccg tctttgtttg gagaaagctg tacaaatgca
      301 ccgaagttca tgatccttac aacttcacct tcgtaaatat ctccaacatt caatggttct
      361 acgatggatt tgatgatgct gatcgcttta tcgatcattg cctgatcttc gcttaagata
      421 tcaatacgtc cactttcgtc aatatcgatc ttaacacctg tttcatcaat gattccattg
      481 atcgttttac cctgtttacc gataacttct gaaatcttct caggatcgat cgtatactga
      541 gagatctttg gagcaaattc tccgacgtgg tctcttgcag ctgggatcac tggattcata
      601 acatcatgaa ggatatgtgt tcttgcttct tttgttcttg caatcgcttc tcggatgatc
      661 tgttctgtca atccatgaat cttgatatcc atctggatcg ctgtgatacc tttgtctgta
      721 cctgctactt taaagtccat atctccaaag aagtcttcta gtccctgaat atctgttaag
      781 atgatgtaat cgtcatctcc ttctcctgtt acaagtccaa cagagatacc tgcaactgga
      841 gctttcactg gaacacctgc tgccattaat gacagagatg aagcacagat acttccctga
      901 gatgtagatc cattagattc catgatctct gatactgtac ggatcgcata tgggaattct
      961 tcttcacttg gtaatactgg aactaacgca cgctctgcta atgcaccatg tccgatctca
     1021 cgacgtcctg gtcctctgct tggtcttgtt tctcctacag agtaagaagg gaagttatag
     1081 tgatgcatat aacgttttgt tgtttctgtt acatcgattc catcaagacg ctgtgcttca
     1141 gataatgatc caagtgttgt cactgtcata acctgagtct gtcctctctg gaataatgca
     1201 gatccgtgaa ctcttggcag gcagtcaact tctgcgtgaa gtggacggat ctgtttgata
     1261 tcacgaccat ctggacgttt gtgatctttt aagatcatct tacggacagt cttcttctgg
     1321 aatgcataaa cagcttcacc gatctgtgct aaccattctt cattctcagc atatctttct
     1381 tctaattttt cttcgatcgc acggatattt gcttctcttg tctgtttgtc atctgtaaat
     1441 acagcttctt ccattgcttc tggagtaatg aaagctacca tatcatcata catctctgga
     1501 tctacgatat gatgctcgta gtcatgtttt tcttttccac attcagctac aattgtatca
     1561 ataaatttga tgatctcctg attcacttcg tgagctttga agattgcttc taacatcaca
     1621 tcttctggta cttcattcgc accagcttcg atcatgataa ctttttctct tgtagatgca
     1681 acagttaatg caagatcaga aacttctttc tgtgcagaag ttgggttgat cacgaattct
     1741 ccatcgatca gtcctaccat tgtagatgct gtaggcccgt caaatgggat atctgaaatt
     1801 gctgttgcga tcgcagaacc aagcattgct gtaagttctg ggctgcaatc tggatctaca
     1861 gacataacta agttgtttaa tgcaacgtca tttctgtagt cttttggaaa cagtggtctc
     1921 attgggcggt cgatcacacg gtctgtaaga attgcattct cagaaggttt tccttctctc
     1981 tttaaatatc cacctgggat cttacctact gcgtataatt tctcttcata ttctacactt
     2041 aatgggaaaa aatcaattcc atctcttggc ttttcagatg ctgttgctgt agataataca
     2101 acagtatcac cgtagtgcat aaatgctgca ccgtttgcct gtgctgctac acgaccgaca
     2161 tcaacgcgaa gtgtacgtcc tgcaagttcc atactaaact gttttgtcat agttcgtctc
     2221 ctttatattt tttgtcttaa ggcgccgaaa caactgaaaa accgactact tgtcttcctt
     2281 catgttgtaa taagcttttc agcagtctcg gaaaacgcct cagtaactta gttagtatag
     2341 catggtttgt ttaaaaaaac cagatgattt ttattttatt ggcgatttca tcgaactttc
     2401 ctataaagta aaaaactgcc gcatgacatt tcatgcggca gtttcaatat cttcattgat
     2461 aagattattt tcttaatcct aatttttcga ttaatgaacg atatctttca acatctttgc
     2521 tctttaagta agctaataag ttacgtctct taccaaccat cttaagaagt cctcttcttg
     2581 aatgatgatc ttttggatgc tgattgaagt gatcctgaag ttcgttgatt ctagctgtta
     2641 agattgctac ctgaacctct ggtgaacctg tatcttttgc gtctttaccg tatttagcta
     2701 cgatttctgc tttcttttct gatgaaatca ttgtgttttc ctccttaaaa ttcttatatc
     2761 gcctgatacc aggaaagggt tggagtgccc aaatctgagt atcggtttca ctaactttta
     2821 tagaatatca taggtttcta tgatttgtaa agaacttttt tatttttctt tcttttttcc
     2881 taattgatgg tttttctttg ctgctgcagt cacagcttcg atcactgcac ttctcattcc
     2941 ttttttctca agaacgttaa ccgcttcgat cgttgttcct gctggagaac atacagcatc
     3001 ttttaattct cctggatgct gtcctgtctc aagaaccatc tttgcacttc ctagtaatgt
     3061 ctgtgcagcg aatgtatatg cctgctttct tggcagtccc tgtgcaactg ctgcatccgc
     3121 cattgcctca ataaacatat aaacataggc tggaccactt ccactgactc caacaacggc
     3181 atccattaga ttctctgtga tcttttccgc ttttccaaaa ctttcaaaaa tcttatgtac
     3241 caggtcaaac tcttcttccg ttacatattc attacagcat agtcctgaca ttccttctcc
     3301 taccagagct ggcgtatttg gcatcacacg tacgatctta acttcttttc caaattgacg
     3361 ctctgctgct tccatagaaa cacctgctgc gatcgttacg atgatctgtt ctgagcttac
     3421 ttcatctctg atctctttga tcacagctgg gtatacctgt ggctttacag ataacactaa
     3481 aatatctgat tgttttgcca cttctttgtt gtctgttgtt gtaatgattc caaattcatc
     3541 ttttagtttc gctgtttttt cttctcgtgg attggatacg atcaattcct gtggacttac
     3601 gactttctgt gtcaaaattc cagtgatcat tgcttgtgcc atatttccac atccgataaa
     3661 tccaagtttc attccgtttt tcctcctatg attgttctaa ttatccagct tattcagtaa
     3721 tttttcaaaa tactctggca aaggcacttc aaactccata tattctccag ttcttggatg
     3781 tacgaatcca aggaccttcg catgaagaca ctgcccttcc agtgtatact ttgacttctt
     3841 tggtccatag acatcatctc ctagcaatgg atgcttgatc gatgccatat gcacacggat
     3901 ctgatgggtc cttcctgtct ctaattgaca ttcgatataa gtaaaacctt tctttagacg
     3961 ttttaatacc ttataatgcg tcactgcatg ttttccatcc atgacgattc ccatcttctt
     4021 gcggtctttt aagcttcttc cgatcggtgc attgactgtc ccttcttctt ccttgatatt
     4081 tccataaacg atcgcatgat attttcttgt gatcgaatga tcatataact gcttggcaat
     4141 gctttggtgt gccatatcgt tcttacagac gatcaggatc cctgttgtgt ctttatcaat
     4201 acgatgcacg attccaggcc ttaacactcc gttaattcct gacagatcat ccttacaatg
     4261 tgccatcaat ccattgacca gcgtaccact gtaatgtcct gctgccggat gcactaccat
     4321 gcctttgggc ttattgatca aaataacatc ctgatcttca tagacaatat caagattcat
     4381 ctcctctggc aaaatctcaa gttctgtcgg ttctggaacc tgtaaggtga tcacatctcc
     4441 ttctttacac ttcttattgc ctttggaagt cttgccattt aatgctgcac gttcttcctt
     4501 acacaattta ttgatataac ttctcgtaag atcgctgcac tgctccgaaa gcaccacatc
     4561 cagacgctta cctgccattt cacttgtcac tttaatttct attgtctcgt ccatgttttc
     4621 ctttcctgtt aagaaaatca agatcttctt ccttatagaa aaataagatc aagatcaaaa
     4681 gtaatatcat tgttacggtc acataaatat ctgcaacatt aaagactgga aaatcaatcc
     4741 atttaaaata aaagaaatca accacatatc ccctgaatac tctgtcgatc agatttccaa
     4801 acgctcctgc catgatcaat gcataactga tacgaaacgg tatatatttc ttcgtctgtg
     4861 gtattctccg atagatccat gttaagatca ctgcagcagc tactgtgatc aatacaaaga
     4921 aaatctgatg atcctgaagg attccaaaag ccgcccctcg attctccaga taagaaagtt
     4981 caaatacatt ggggatcaca gcgatccctt ctgtacctcg aagtgcagat aatgcccaga
     5041 ctttacttaa ctgatcgatc aagatcagcc ctatgatata tatataaata cgaatctgtt
     5101 tcttcatgtc tcgtccttaa aaactcatcc c
//
LOCUS       DAOMUE010000133         5110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4507_length_5110_cov_2.100495, whole genome shotgun sequence.
ACCESSION   DAOMUE010000133 DAOMUE010000000
VERSION     DAOMUE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4507_length_5110_cov_2.100495"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /gene="uppS"
                     /locus_tag="PLC71_06790"
     CDS             <1..517
                     /gene="uppS"
                     /locus_tag="PLC71_06790"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRM02383.1"
                     /translation="KRPQDEVNALMKLLRDYLKTCIKRANKNNMKVRVIGDITGLSED
                     LQEKIVELEKASKDNTGINFTIALNYGSRDEMVRAMRHMADDLSSGNLSKDEITEEVF
                     NGYLDTKELPDPDLMIRTSGEERLSNFMLWQLAYTEFYFTDVLWPDFNKKELIKAIEY
                     YNGRDRRFGGI"
     gene            522..1334
                     /locus_tag="PLC71_06795"
     CDS             522..1334
                     /locus_tag="PLC71_06795"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRM02384.1"
                     /translation="MFKQRLISGIILVLIAAFGLYMGGIVTFAMTLIVALIGYSEILK
                     IYHIEKSSLAVIGYTGTILYYVALLFDLDQWTTAIIILFLIFLLGCYVVRFPKYKVDQ
                     MMAGFFGFVYVAVMMSFIYQIRELQNGGEFVVLLFLAAWGNDTLAYCTGMLIGKHKMS
                     PILSPKKSIEGLVGGIVGAAILGALYGLYLNTYIRFSFNPVTMFPIVCGLGGAVSVIG
                     DLAASAIKRDSGIKDYGTLIPGHGGILDRFDSIIFIAPVIYYLTVMISGGAI"
     gene            1336..>2159
                     /gene="dxr"
                     /locus_tag="PLC71_06800"
     CDS             1336..>2159
                     /gene="dxr"
                     /locus_tag="PLC71_06800"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRM02385.1"
                     /translation="MKYISIIGSTGSIGTQTLDIVRSNKDLKVTALAAGTSIDLLEKQ
                     AREFQPVIVAVYNEQKAEELKLRLADTNIKVCAGMDGLLEAATQEQCSVVVTAIVGMI
                     GIRPTIAAMKAGKDIALANKETLVTAGHIIMPMAEELGVSIYPVDSEHSAIFQCLQSG
                     KRDDLDSLIITASGGPFRKKTTEELKHVTVEDALNHPNWSMGRKITIDSATLVNKGLE
                     VIEAKWLFGVDFDDIHVVVQPKSVIHSMIQFKDGSVIAQLGTPDMKLPIQYALFYP"
     assembly_gap    2160..2169
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2170..2496
                     /locus_tag="PLC71_06805"
     CDS             <2170..2496
                     /locus_tag="PLC71_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRM02386.1"
                     /translation="YGGERRFLDGERLDFAKLKEITFEEPPVDVLKGLPYAYKAGRIG
                     GSMPTVLNAANEKAVALFLDRKIQFLDIYDIIEDTMNAHKVIENPTLEEVLETEQWVY
                     EHIESR"
     gene            2501..>3465
                     /locus_tag="PLC71_06810"
     CDS             2501..>3465
                     /locus_tag="PLC71_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRM02387.1"
                     /translation="MNIIIAILIFGIIIIVHELGHFLIAKKNGIQVDEFCIGLGPTII
                     GKQVGETFYSVKLLPFGGACMMGEDEDRPGENAFNNKSVWARMAVIFGGPFFNFILAF
                     IFSIIVIGMSGADIPKISKVEKDSPAYEAGIRKGDTMIKVAGKKMHNYREFSYYMYLD
                     YDGGKIPITILQNGKEKNINVTPEYDKERGQYLIGITWNGYQKVGPLKTIEYSFREVG
                     LQVKITLKSVKMLVSQKLGVKDLSGPVGIVKTVGDQYTQAAAYGFKTVFLTMVNWIIL
                     ISANLGVMNLLPLPALDGGRLLFLIIEAITGKAVPQNMEALVHTAG"
     assembly_gap    3466..3475
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3553..4614
                     /gene="ispG"
                     /locus_tag="PLC71_06815"
                     /gene_synonym="gcpE"
     CDS             3553..4614
                     /gene="ispG"
                     /locus_tag="PLC71_06815"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRM02388.1"
                     /translation="MYTRNQTRVISIGDVKIGGGNPVAIQSMTNTKTENVEETVKQIL
                     ALEKAGCQIIRCTVPTMKAAEAFTEIKKQIHIPLVADIHFDYKLAIAAMEHGADKIRI
                     NPGNIGGEENICKVVETAKKYHVPIRVGVNSGSLEKPLVKKYNGVTAQGLVESALDKV
                     AMLEKYDFHDMVVSIKSSDVLMCAEAYRLVADKINYPLHVGITEAGTVFSGNIKSAVG
                     LGIILNQGIGDTIRVSLTGDPVNEIASAKLILKTLGLRSGGIEVVSCPTCGRTNIDLI
                     PLANQVETMAADFENLNLKVAVMGCVVNGPGEAKEADVGIAGGNGEGLLIKKGEVIRK
                     VPEDQLLDTLRYELEHWGE"
     gene            4616..>5110
                     /locus_tag="PLC71_06820"
     CDS             4616..>5110
                     /locus_tag="PLC71_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III N-terminal
                     domain-containing protein"
                     /protein_id="HRM02389.1"
                     /translation="MEVTTKPFFSVFPDLKVSDDVRSLFEDANIREITLKKKENTLKI
                     SFQCDHLISRVDTWRMERTIREQLFSKRFVKIRFQESYHLSEQYNLKYLMEHYMKSFL
                     FELKGKGEVIFNVVRKGDYRIEDDVITFYFEDTFVNRNKAPEIKEFFEMILKQSSPQE
                     TEREE"
BASE COUNT         1680 a          747 c         1192 g         1471 t
ORIGIN      
        1 gaagcgccct caggatgaag tgaatgcact gatgaaatta cttcgtgatt atttaaaaac
       61 atgcattaag cgtgcaaata aaaataatat gaaagtcaga gtaatcggag atatcacagg
      121 actttccgaa gatttacagg agaagatcgt tgagcttgag aaggcatcca aagataatac
      181 agggatcaat tttacgatcg cactgaatta tggaagtcgt gatgagatgg tgcgagctat
      241 gagacatatg gcagatgatc tttctagtgg aaatctttcc aaagatgaga tcacagaaga
      301 agtgtttaat ggatatctgg atacaaaaga acttccagat ccagatctga tgatccgtac
      361 aagtggggaa gaaagattgt cgaactttat gttatggcag ttagcatata cagaatttta
      421 ttttacagat gtgctctggc ctgattttaa taaaaaggaa ttaataaagg cgatcgaata
      481 ctataatgga cgcgaccgca gatttggagg aatataagtt tatgtttaag caaagactga
      541 tcagtgggat cattttagta ttgatcgcag cttttgggtt atatatgggt ggaattgtga
      601 cgtttgcaat gacattgatc gttgcattga tcggatacag cgagatttta aagatttatc
      661 atattgagaa atcatctttg gcagtgatcg gatatacagg cacgattctt tattatgtgg
      721 cattgttatt tgatcttgat caatggacca cagcgatcat tattttattt ttaatctttt
      781 tactcggatg ctatgtggta cgttttccaa agtataaagt ggatcagatg atggcaggat
      841 tctttggatt tgtatatgta gcggttatga tgtcctttat ctatcagatc cgtgaattac
      901 aaaatggagg agaatttgtt gtattattat tccttgcagc atggggaaat gacactttag
      961 catattgtac aggaatgttg atcggaaaac ataagatgtc accaatttta agtcctaaga
     1021 aaagtataga aggtttggtt ggcggaattg tcggtgcggc aattcttggt gcattatatg
     1081 gattatatct aaacacatat atcagattta gttttaatcc agttacaatg ttcccaattg
     1141 tttgtggact aggtggagca gtttcagtca ttggagattt ggcagcatct gcgatcaaga
     1201 gagattcagg aattaaggat tatggtacat tgatccctgg tcatggagga attcttgacc
     1261 gatttgatag tattattttt attgcacctg tcatttatta tctgacagtt atgatctcag
     1321 gaggagcgat ctagaatgaa atatatttca attattggat ctacaggttc cattggaacc
     1381 cagacactag atatcgtacg aagtaataaa gatctgaaag ttacagcctt agcagcagga
     1441 acaagtattg atctgttaga gaaacaagca agagaatttc agccagtgat cgttgctgta
     1501 tacaatgaac agaaggcaga agagttgaaa cttcgtctgg cagatacgaa tataaaagta
     1561 tgtgcaggaa tggatggatt attagaagca gcaacacagg agcagtgttc tgttgttgtc
     1621 acagcaattg tcggaatgat cggaattcgt ccaacgatcg ctgcgatgaa ggctgggaaa
     1681 gacatcgcac ttgctaataa agaaacattg gtaactgcag ggcatatcat tatgccaatg
     1741 gcggaagaat tgggtgtttc tatttatcca gtagatagtg aacattctgc aatctttcag
     1801 tgtttacaga gcgggaaaag agatgatctt gattctctga tcattacagc atcaggggga
     1861 ccgttccgta agaaaacgac agaagaatta aaacatgtga cagtagaaga tgcgctgaat
     1921 catccaaatt ggtcaatggg acgaaagatt acgattgatt ccgcaacgtt agtgaataaa
     1981 ggactcgaag tgatcgaagc caaatggttg tttggagtgg attttgatga tattcatgtt
     2041 gtagtacaac caaagagtgt gatccattct atgattcagt ttaaggatgg aagtgtgatc
     2101 gcacagcttg gaacaccgga tatgaaatta ccgattcaat atgcattatt ttatccacan
     2161 nnnnnnnnnt atggcggaga acgtcgcttt ttagatggag aacgtctgga ttttgcaaaa
     2221 ttaaaagaaa ttacatttga agaaccacca gtggatgttc taaaaggact tccttatgca
     2281 tataaagcag gtaggatcgg tggaagcatg ccaacagtct taaatgctgc aaatgaaaaa
     2341 gcagttgcat tatttttaga ccgtaagatt cagtttttag atatttacga catcattgaa
     2401 gacacaatga atgcccataa agttattgag aatccgacac tggaagaagt gttagagaca
     2461 gagcaatggg tgtatgaaca catagaaagc aggtagatca ttgaatatta tcattgcaat
     2521 tttaattttt ggaattatta ttattgtgca tgaattaggg cattttctga tcgctaagaa
     2581 aaatgggatt caggtagatg aattttgcat tggacttgga ccaacgatta taggaaagca
     2641 ggttggagag actttttatt ctgtcaaact gctgccattt ggtggtgcat gtatgatggg
     2701 agaagatgaa gatcgacctg gagaaaatgc gtttaataat aagtccgtct gggcaaggat
     2761 ggcagttatt tttggaggac cattttttaa ctttatctta gcgtttatat tttcaattat
     2821 agtgatcgga atgtctggtg ctgacattcc gaagatttcc aaagtagaaa aagattcacc
     2881 agcatatgaa gcaggaatcc gtaaaggaga tactatgatc aaagttgctg gaaagaaaat
     2941 gcacaattac agagagtttt cctattatat gtatttagat tatgatggag ggaaaattcc
     3001 gatcacgata ttacaaaacg gaaaagaaaa aaatataaac gtcacaccag agtatgataa
     3061 agaacgaggg caatatctga tcgggatcac ctggaatgga tatcagaaag taggtccgtt
     3121 aaaaacgatc gaatacagtt tccgagaagt aggattgcag gtaaagatta ctttaaagag
     3181 tgtaaagatg ttagtttcac aaaaacttgg tgtgaaagat ctttcaggac cagtaggaat
     3241 tgtcaaaacg gtcggagacc aatatacaca ggctgctgcc tatggattta aaacagtatt
     3301 tttgacaatg gtaaactgga tcattttgat cagcgcaaac cttggtgtga tgaatctatt
     3361 gccattacca gcacttgatg gaggtcgttt attgtttttg atcatagaag caattactgg
     3421 aaaggctgtg ccacagaata tggaagcgct ggtacacact gcaggnnnnn nnnnntgatc
     3481 ttattaatgc ttttgatggt atttgtaatg tatcaagata ttgtaaagat tttcatgtaa
     3541 agggggattt gcatgtatac aagaaatcag acaagagtta tttcgattgg agatgttaag
     3601 attggaggag gcaatccagt tgccatccag tctatgacaa atacaaagac agaaaatgtg
     3661 gaagagacgg tcaaacagat tcttgctctt gagaaggctg gatgccagat cattcgctgt
     3721 actgttccaa caatgaaagc tgccgaagct tttacagaga ttaagaagca gattcatatt
     3781 ccgttggtcg cggatattca ttttgactat aaacttgcaa ttgcagcgat ggaacatggg
     3841 gcagataaga tccgtatcaa tccggggaat atcggtggag aagagaatat ttgtaaagtg
     3901 gtagaaacag caaagaaata tcatgtaccg atccgcgttg gtgtaaacag cggatcattg
     3961 gagaaaccac ttgttaaaaa atataacgga gtaactgcac aaggactggt agaaagtgcc
     4021 ttagataaag ttgctatgct tgaaaaatat gattttcatg atatggtagt cagtattaag
     4081 tcttctgatg ttttgatgtg tgcagaagca taccgcctag tcgcagacaa aatcaattac
     4141 cctctgcatg tagggattac agaagctgga acagttttta gtggtaatat caaatcagct
     4201 gtaggacttg gaattatctt aaatcaggga attggtgata cgatccgtgt atcattgaca
     4261 ggagatccag tcaacgagat tgccagtgcg aaactgatct taaagacatt aggacttcgt
     4321 tccggaggaa ttgaagttgt atcctgccca acatgtggac gtacgaatat cgatctgatt
     4381 ccacttgcca atcaggtaga gacaatggca gcagattttg aaaatctgaa tcttaaggtt
     4441 gctgttatgg ggtgcgttgt aaacggacca ggagaggcca aagaagcaga tgtgggaatc
     4501 gctggtggaa atggagaggg acttctgatc aagaaaggcg aggttatccg taaagtacca
     4561 gaagatcaat tacttgatac tttaagatat gaattagaac actggggtga ataaaatgga
     4621 agtgacaaca aaaccatttt tcagtgtatt tccagatttg aaagtttctg acgatgtaag
     4681 atctttgttt gaagatgcga atattagaga gatcacacta aagaaaaaag aaaacacgct
     4741 taagatttct tttcagtgtg atcatctgat ctcaagagtc gatacatgga gaatggaacg
     4801 cacgatccgg gaacagctgt tttcaaagcg ttttgtaaag attcgttttc aggaaagtta
     4861 tcacctgtca gaacaatata atctcaaata tctaatggag cattacatga aaagcttcct
     4921 ctttgagtta aaaggcaaag gggaggttat ttttaatgtc gtgagaaaag gggattatag
     4981 aattgaagat gatgtgatca ccttttactt tgaagataca tttgtaaacc gcaataaagc
     5041 cccagagatc aaagaatttt ttgagatgat tttaaaacaa tccagcccgc aggaaacaga
     5101 aagagaggaa
//
LOCUS       DAOMUE010000134         5100 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4516_length_5100_cov_2.426561, whole genome shotgun sequence.
ACCESSION   DAOMUE010000134 DAOMUE010000000
VERSION     DAOMUE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4516_length_5100_cov_2.426561"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1362)
                     /gene="accC"
                     /locus_tag="PLC71_06825"
     CDS             complement(16..1362)
                     /gene="accC"
                     /locus_tag="PLC71_06825"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRM02390.1"
                     /translation="MFRSILIANRGEIAVRIIRACRNMGIKSIAVYSKEDKDSLHVQL
                     ADRRVCIGEGPAKNSYLNMERIISAAKNTDADAIHPGFGFLSENADFVRLCKENGIAF
                     IGPSAEVIDSMGNKSNARKTMMEAGVPVVPGTKEPVYDAATGEKLADEIGYPVMIKAS
                     SGGGGKGMRVSRSKEDFEFNFNTAQRESANAFGDDTMYIEKFIENPRHVEIQIMADGH
                     GNVVALGERDCSVQRNHQKLIEESPSPAITEKIRKSMNHDAILAAKAVGYTNAGTIEF
                     ILDPKGTYYFMEMNTRIQVEHGVTEMVTGTDLIIEQIRVAMGMELSFTQEDVEINGHA
                     IECRINAEIPEKNFMPSPGVIKHLHLPAGNGVRVDTAIYTGYCIPSEYDSMIAKVIVH
                     APDRDAALQKMRSALDEMVILGINTNLDFQYQLMNNREFCEGKADTGFIERLLKLD"
     gene            complement(1375..1806)
                     /gene="accB"
                     /locus_tag="PLC71_06830"
     CDS             complement(1375..1806)
                     /gene="accB"
                     /locus_tag="PLC71_06830"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRM02391.1"
                     /translation="MNLDKIEKLVKIIENSSMLDFSIQEGDVKIKMSRRGSVGAPAAP
                     MSVSTQGVDTVESIDSEDESYITSPIVGTFYAAPSPDAKAFVKVGDRVKAGQTVCILE
                     AMKLMNEIESDYDCEIEAVLASNEQKVEYGQPLFKVKKLED"
     gene            complement(1824..2828)
                     /locus_tag="PLC71_06835"
     CDS             complement(1824..2828)
                     /locus_tag="PLC71_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HRM02392.1"
                     /translation="MGIRVLKGGMMTTVQDLGRTGYQSQGFSVSGVMDVRSFKIANLL
                     LDNPENEAVLEFTLIGPTLEFTSETIISITGGDFRPQINGKPAKMYTAIYMHRGDILT
                     FGGARTGTRGYIAFSSYLDVPVVMGSRSTNIKCQIGGYKGRKLEDEDYIAFRAKRRYL
                     PYYLSRSLDLDEFDQEKITLRVVMGPQEKMFTKEGRETFLNSEYTVTSEFDRMGCRLE
                     GPFIAYKTTSDIISDGIAFGSVQVPSHGKPIILLADRQTTGGYAKIATVVSVDIPKLV
                     QRKTEQKVRFQAVSVEEAQKLLADEMKELNDFRSQIYTPCKEVLDCRLVAKRISTLFK
                     "
     gene            complement(2832..3560)
                     /gene="pxpB"
                     /locus_tag="PLC71_06840"
     CDS             complement(2832..3560)
                     /gene="pxpB"
                     /locus_tag="PLC71_06840"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HRM02393.1"
                     /translation="MEEVKISTAGDSALLIEFGQEISTEINARITAFVHLLKAQRIEG
                     VQDMIPAFTSLLINYDPRVVNYKTLTKRLQKLLKLDVNEETSTSRVFEIPVCYGGEYG
                     PDIENIAKNAGLTEEEVIKIHSSKDYLIYMLGFLPGFSYLGGLDERIHTPRLANPRIR
                     IPAGSVGIGGSQTGIYPLDSPGGWQLLGLTPVKTYDPGRENPILFEAGDYIRFVPVSE
                     EEYLKIKEQVENGTYECIIHTKEV"
     gene            complement(3637..4860)
                     /locus_tag="PLC71_06845"
     CDS             complement(3637..4860)
                     /locus_tag="PLC71_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HRM02394.1"
                     /translation="MKKENNKKSASALIGAAFLMATSAIGPGFLTQTGKFTDSLHGSF
                     GFVILISIILAAIVQLNVWRVLCVSGMRGQDVANKVLPGLGYVIAFLVVAGGLVFNIG
                     NVGGGALGFNTLLGIPTTVGYFIAGAIAIIVFLSKNALNAMDNLTKILGGIMILVIFI
                     VILIVQPPVGMAAKETIMPTASMGDIFPAILTLLGGTVGGYITFAGAHRLIDSGITGK
                     ENLKEINKSSVMGMGIATIVRIFLFLAVLGVVVATATSPAHTLDAANPTADAFLQGAG
                     QIGYRFFGLVILCAAITSIVGCAYTSVSFLKTFSKTIEKNEKWFIVGFIAISTVCMAL
                     AGQPAVLLVLAGALNGLILPITLGVCLIASQKKSVMGEDYHHPVLLLVLGIVVVVVSG
                     YLGITSLSSLSALFA"
BASE COUNT         1399 a         1193 c          845 g         1663 t
ORIGIN      
        1 tccgttttat cgaatttaat ctaatttcaa gagtctctcg ataaatcctg tatccgcttt
       61 tccttcacag aattcacgat tattcatcag ctgatactgg aaatcaaggt ttgtattgat
      121 ccctaatatc accatctcat caagagctga tctcattttc tgtaatgctg catcacggtc
      181 tggtgcgtgt acgataactt tcgcgatcat agaatcgtat tcggatggaa tacagtaccc
      241 tgtataaatt gcggtatcta cacgaactcc atttccagct ggcaaatgca aatgtttgat
      301 cacacctgga cttggcataa agtttttctc tggaatctct gcattgatac gacattcgat
      361 cgcatgtccg ttgatctcca catcttcctg tgtaaaactt aattccattc ccatagccac
      421 tcggatctgt tcaatgatca gatctgttcc cgtaaccatc tctgtgaccc catgttctac
      481 ctgaatacgt gtgttcattt ccatgaaata ataggttcct tttggatcaa ggataaattc
      541 tatggttcca gcattggtat accctactgc tttcgctgca aggatcgcat cgtgattcat
      601 agattttctg atcttttccg tgatcgctgg ggatggtgat tcttcgatca gtttctggtg
      661 gtttctctga acagaacaat cacgttctcc caaggctacg acattcccat gtccatctgc
      721 catgatctga atctccacat gtcttggatt ctcgatgaat ttctcaatgt acatcgtatc
      781 atcaccaaat gcattggcag attctctctg cgcagtattg aaattgaatt caaaatcttc
      841 tttggatctg gaaactcgca ttccttttcc accaccacct gaagatgctt ttatcataac
      901 tggataaccg atctcatctg ctaatttctc ccctgtagct gcatcataga caggttcttt
      961 ggttcctgga acaactggaa ctcctgcttc catcattgtt tttcttgcgt ttgatttatt
     1021 tcccatgctg tcaatgactt ctgcactcgg tccgataaat gcaataccat tttctttaca
     1081 taagcgaacg aaatcagcat tctcagataa gaaaccaaac cctggatgga tcgcatctgc
     1141 gtccgtattc tttgctgcag aaatgatacg ttccatgttt aagtagctgt tttttgcagg
     1201 tccttctccg atacatactc ttcgatctgc taattgtaca tgaagactgt ctttatcttc
     1261 ttttgaataa accgcgatac tcttgatccc catattacga caagcacgaa taatgcgcac
     1321 tgcgatctca ccacggttcg cgatcaatat acttcgaaac atacaatgcc tcctttagtc
     1381 ttcaagtttt tttactttaa ataatggctg tccatattca actttctgtt cgttgcttgc
     1441 taagacagct tcaatctcac agtcataatc actttcgatc tcattcatta atttcatcgc
     1501 ctcgaggata catacagtct gtcctgcctt gactctgtct ccaaccttca caaatgcctt
     1561 ggcatctggg gatggagccg cataaaaggt acctacgatc ggagatgtga tgtatgattc
     1621 atcttcggaa tcaatacttt ctactgtatc aacaccctgt gtggaaactg acatcggtgc
     1681 agcaggcgct ccaacacttc ctcttctgct cattttgatc tttacatcgc cttcctgaat
     1741 gctgaaatca agcatagagg aattctcgat gatctttact aatttctcaa ttttatctaa
     1801 attcaccgtt tcattctcct tttctattta aatagtgtgc tgattcgttt tgcaacgagt
     1861 ctgcaatcta atacttcttt acaaggtgta taaatctggc ttctgaagtc attcagctct
     1921 ttcatctcat ctgctaacag cttctgggct tcttctacgg atactgcctg aaatcttact
     1981 ttttgttctg tttttctttg aacaagtttc ggaatatcta cagaaacaac tgtcgcgatc
     2041 ttcgcatatc cacccgttgt ctggcggtct gctaataaga tgatcggttt tccatgactt
     2101 ggtacctgaa cagatccaaa ggcgattccg tcagatatga tgtctgaagt tgtcttatat
     2161 gcgataaatg gtccttctaa tcgacatccc atgcggtcaa attcacttgt tactgtatat
     2221 tctgaattta aaaatgtttc tcggccctct ttcgtaaaca tcttttcctg aggtcccatg
     2281 acaactctta atgttatctt ttcctgatca aattcatcaa gatccagact tcttgataaa
     2341 taatatggca gatatcttcg ctttgccctg aacgcaatat aatcttcatc ttctagtttc
     2401 cggcctttat atccaccgat ctgacatttg atattcgtag aacggcttcc catgactact
     2461 ggcacatcca gataacttga aaatgcaata tatcctcttg ttccagttct tgctccgcca
     2521 aatgtcaaaa tatctccacg atgcatataa attgctgtat acatcttggc aggttttcca
     2581 ttgatctgcg ggcggaaatc tccacctgtg atcgaaatga tcgtctcgga tgtaaattca
     2641 agtgttggtc ctattaaggt aaattcaaga actgcttcat tttccgggtt atccagcagg
     2701 agatttgcaa tcttaaaaga acgaacatcc ataactccgg atacactaaa tccctgactc
     2761 tggtatcctg ttcttccaag atcctgcacc gttgtcatca ttccgccttt taatacacga
     2821 attcccataa tctacacctc ctttgtatga atgatacatt catatgttcc attttcaacc
     2881 tgttctttga tctttaaata ctcttcttct gaaacaggta caaaacggat ataatctcca
     2941 gcttcaaaaa gaattggatt ctctcgtcct ggatcatagg tttttactgg tgtcagacca
     3001 agtaactgcc atcctcctgg tgaatcaagc ggataaattc ctgtctgaga gccaccgatc
     3061 ccaacacttc ctgccggaat tctgattctt gggttcgcca gtcttggtgt atgaattctt
     3121 tcatcaagtc ctccaagata tgaaaatcct ggcaaaaatc caagcatgta gatcaagtag
     3181 tctttggaag agtgaatctt tataacctct tcttccgtta aaccagcatt ttttgcgata
     3241 ttttcaatat ctggtccgta ttctcctcca taacatactg ggatttcaaa aactctggat
     3301 gtcgatgtct cttcattgac atccagtttt agtaacttct gtagacgttt tgttaatgtt
     3361 ttgtaattta ccaccctcgg gtcataattg atcaagagac tggtaaatgc cgggatcata
     3421 tcctgcacac cttcgatacg ctgcgctttt aaaagatgta caaaggctgt gatccttgca
     3481 ttgatctctg tggaaatttc ctgtccaaat tcaatgagta aggcagaatc tcccgcagta
     3541 ctaatcttta cttcttccat gttttttcac ataactcctt tcggtcagtc tgtgcttcta
     3601 tgttttaaac ttatctgttt atccagacaa ttttgcttat gcaaataatg cacttaagct
     3661 gcttaatgat gtaattccaa gatatcctga aacaacaact accacgattc caagaaccag
     3721 taataatact ggatgatgat aatcttctcc cataacagat ttcttctgag atgcgatcag
     3781 gcaaactcct aatgtgatcg gtaagatcaa tccatttaat gctcctgcaa gtactaataa
     3841 taccgctggc tgtccagcaa gtgccataca tacagtactg atcgcgataa atcctacaat
     3901 aaaccatttc tcattcttct caatcgtttt gctgaatgtc ttaaggaaag acacagatgt
     3961 ataagcacat ccaacgatgg atgtgatcgc tgcgcataag atcacaagtc caaagaatct
     4021 ataaccgatc tgtcctgctc cctgtaaaaa tgcatccgct gttgggttcg ctgcatctaa
     4081 tgtatgagct ggtgaagttg ctgttgctac aaccactcct aaaactgcaa ggaataagaa
     4141 gatacgtacg atcgttgcaa ttcccattcc cataacagaa ctcttattga tctcttttaa
     4201 gttctctttt ccagtgattc ctgaatcgat cagacgatgt gcacctgcaa atgtaatata
     4261 tccaccgact gttcctccaa gtaatgtaag gatcgctggg aaaatatctc ccatagatgc
     4321 tgttggcatg attgtttctt ttgctgccat accaactggc ggctgtacga tcaggatcac
     4381 aataaagatg acaaggatca tgatcccacc aaggatcttt gtaaggttat ccatagcatt
     4441 taatgcattc tttgataaaa atacgatgat cgcaattgct cccgcaataa aatatccaac
     4501 agtagtagga attccaagaa gtgtattaaa acctaaagca cctccaccaa cgttaccgat
     4561 attaaataca agaccacctg ctacaactaa aaatgcgatc acatatccaa gtccaggaag
     4621 taccttattc gccacatcct gtccacgcat tccagatacg cacagaactc tccatacatt
     4681 taactgcacg atcgctgcca gtatgataga aatcaaaatt acaaatccaa agcttccatg
     4741 cagactatct gtaaatttac cagtttgtgt caaaaatcca ggtccaattg ccgaagttgc
     4801 cataagaaat gccgcaccga tcaaagcgct tgcacttttt ttgttattct ctttcttcat
     4861 agtaaataat ttcctttcct tttcgtaata attaataatt ataacaaaat ttctgacgat
     4921 acgcaacttt aaagcgttga tttgaataac gttcaacata cacaactact taaattatca
     4981 atcttgtaat tgtccacact ctagacttct ggtatatgat aaaaaataaa taaacgatca
     5041 gaagagcagc attagaattt tgccacgttt tttacggatt cgcagccggc ttggtgcgtt
//
LOCUS       DAOMUE010000135         5048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4592_length_5048_cov_2.269778, whole genome shotgun sequence.
ACCESSION   DAOMUE010000135 DAOMUE010000000
VERSION     DAOMUE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4592_length_5048_cov_2.269778"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLC71_06850"
     CDS             complement(<1..737)
                     /locus_tag="PLC71_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HRM02395.1"
                     /translation="MKVTAFLIKYGEIAIKGKNRYMFEDALMKQIRHAMAPVGDFKVR
                     KESGRMFVEAQDEFDYDEAVEALQRVFGIVGICPMIIEENKDMEHLTETVIKFIDEQY
                     PVKDFTFKVHARRGDKQFPLDSMAINMEIGGQLLDAFEGIKVDVHHPQVMVNIEVRKY
                     IYIYSQEIKGPGGMPVGTNGKAMLLLSGGIDSPVAGYMIAKRGVHIDATYFHAPPYTS
                     ERAKQKVIDLAKIVAKYAGPINLHVVN"
     gene            complement(781..1923)
                     /locus_tag="PLC71_06855"
     CDS             complement(781..1923)
                     /locus_tag="PLC71_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRM02396.1"
                     /translation="MIYFDNAATTCVFPEVVEAMQDAMQVTYGNPSAKHMKGIEAENK
                     VKAAQELIAKTLRAKPKEILFTSGGTESNNTAIIGTAMANRRKGKHIITTKVEHASVY
                     EPMYHLEEEGYEVTYLDVDSEGIVDLDQLEESIREDTILVSCMMVNNEIGAIEPIEEI
                     AKIIKAKNPNTIFHVDAIQAYGKLPIVPKNMGIDLLSTSGHKIHGPKGIGFLYIKEGT
                     KISPIIFGGGQQKGMRSGTENVPGIVGLAVACEKMIGKNYENAEKIREVKEYFQERIK
                     EIEDIKDNSGKAPHVASISFKKLRSEVLLHALEDREIYVSSGSACSSNKKQAVSGTLN
                     AIALSDEFKDGTLRFSFSIENTKEEVDETIKALKELVPMLRRFVRK"
     gene            complement(1938..2675)
                     /locus_tag="PLC71_06860"
     CDS             complement(1938..2675)
                     /locus_tag="PLC71_06860"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRM02397.1"
                     /translation="MYRFFIKEEQIHDGMIEICGSDVNHIKNVLRMKTGDKVYLSNGS
                     DLEYECSLLEWTDDTILAKIEDVHGMETELPVKITLYQGLPKGDKMEMIVQKAVELGV
                     AEIVPVAMKRCVVKLDAKKAAKKVSRWNTIALSAAKQAKRGIIPEVREVRNFKDILEE
                     VQDIEFMLVPYEEAKGMQASKELISQAKGKKSIGIIIGPEGGFEKEEIEQLKAAGGQT
                     MSLGKRILRTETAGMTVLSILMFTIEE"
     gene            complement(2679..3638)
                     /gene="prmA"
                     /locus_tag="PLC71_06865"
     CDS             complement(2679..3638)
                     /gene="prmA"
                     /locus_tag="PLC71_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRM02398.1"
                     /translation="MGWNKITIDTLTSAEDMISYELTELGATGVEVEDHVSLTEEEMK
                     VMYVDLLPDDIAPDDGKAKISCYFDENENLEHITMQIQAMLERLRGFMDIGAGTISFD
                     KTKEEDWVNNWKKFFKPIRLDEQIVIKPTWETLEDQTEDMIVVEIDPGTAFGTGSHET
                     TKLCIEGMKPYIKEDTKILDVGCGSGILSIIGLKLGAGHAVLTDIDPHAISASEENFE
                     VNHISKDQFEVYQGNVLDDKEFAASLGSKCYPVVVANILADVISPLIEIVDQFLAEDG
                     VFVISGIIDTKEKEIADKLNAYGFDVIETRRMKDWVSMTAKMR"
     gene            complement(3815..4546)
                     /locus_tag="PLC71_06870"
     CDS             complement(3815..4546)
                     /locus_tag="PLC71_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM02399.1"
                     /translation="MIRRIIEIDEDKCNGCGLCADACHEDAIGIVDGKAKLLRDDYCD
                     GLGDCLPNCPTGAISFVEREAAAYDREAVEANMKKKAEMTAKDKIEEKPVFHGCPGTR
                     MKIMDHKAEEVATEQTATVSSQLSQWPVQIKLVPVNAPYFKDANLLIAADCTAYAYGN
                     FHQKFIKNKVTLIGCPKLDEGDYSEKLTQIIANNDIKSVTIVRMEVPCCGGLENAAKK
                     AIQDSGKFLPWQVHTISIDGKILEA"
     gene            complement(4742..>5048)
                     /locus_tag="PLC71_06875"
     CDS             complement(4742..>5048)
                     /locus_tag="PLC71_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02400.1"
                     /translation="NTVCEKENFKMIRMKENYYIKKYYGDFVLLKRAKNKENCKKVLE
                     KVGVYGTLREVKEAIIKEMIKDEVNHRKTEPLYKLIPLMRQMYNRFYEENMECCFVG"
BASE COUNT         1265 a         1185 c          742 g         1856 t
ORIGIN      
        1 aagtttacaa catgtaaatt aatcggtcct gcatattttg caacgatctt cgcaagatct
       61 ataaccttct gttttgctct ctcgcttgtg tatggtggtg catggaaata tgttgcatcg
      121 atgtgtactc cacgttttgc gatcatatat cctgcaactg gactgtcgat tcctccggat
      181 aataaaagca tcgcttttcc attggttccg actggcattc cacctggtcc tttgatttcc
      241 tgagaataga tatagatata ttttctcact tcgatattca ccattacctg tggatggtgc
      301 acatctactt tgatcccttc aaatgcatct agtaactgcc caccgatttc catattgatc
      361 gccatggaat caagagggaa ctgtttatct cctcttcttg catgaacctt gaatgtaaaa
      421 tccttgactg gatactgttc atcaataaat ttgataactg tctctgtcaa atgttccata
      481 tctttatttt cttcgatgat cattggacag attccaacga ttccaaagac tctctgcagt
      541 gcttccacag cttcatcata atcgaactca tcctgtgctt cgacaaacat acgtccagat
      601 tcttttctta ctttaaaatc cccaacgggt gccattgcat ggcggatctg tttcattaat
      661 gcgtcttcaa acatataacg gttcttacct ttgatggcaa tctcaccgta tttgatcaaa
      721 aatgcggtta ctttcatagt attctccttt attttcttac tgtttctttt cttgtggtct
      781 ttattttctt acaaatcttc gaagcattgg tactaattct tttaatgctt tgatcgtttc
      841 atcaacttct tcttttgtat tctcaattga gaaactgaaa cgaagtgttc catctttaaa
      901 ttcatcactt aatgcgatcg catttaatgt tccactgact gcctgttttt tgttcgatga
      961 acatgcggat cctgaagata catagatttc tctgtcttcc aaggcatgta acagtacttc
     1021 acttcttaac ttcttaaagc tgatacttgc cacgtgtggt gcttttccag aattgtcttt
     1081 gatatcctca atctctttga tcctttcctg aaaatattct ttgacttcac ggatcttctc
     1141 agcattttca tagtttttac cgatcatctt ttcacatgcg acagctaatc ctacgattcc
     1201 tggtacattt tccgttccag aacgcattcc tttctgctgt ccgcctccga aaatgatcgg
     1261 agagatcttc gttccttcct tgatatataa aaatccgatt ccttttggtc catggatctt
     1321 atgaccactt gtggataata agtcaattcc catattcttt ggaacgatcg gaagttttcc
     1381 atatgcctga atcgcatcga catggaaaat cgtattcgga ttctttgctt tgatgatctt
     1441 tgcgatctct tcaattggtt caatggctcc aatctcgtta tttaccatca tgcaagatac
     1501 aaggatcgta tcttctcgga tactttcttc taactgatca agatctacga ttccttctga
     1561 atcaacatca agatatgtca cttcatatcc ttcttcttcc agatgataca ttggttcata
     1621 tacagaagca tgttctacct ttgtcgtaat aatgtgcttt cctttgcggc gatttgccat
     1681 tgctgttcca atgatcgctg tattgtttga ttctgttccc ccagatgtaa ataagatttc
     1741 tttgggtttg gctcttaatg tcttcgcaat taattcttgt gcagctttta ctttattctc
     1801 tgcttcaata cctttcatat gttttgcaga tggattccca tatgtgacct gcattgcatc
     1861 ctgcattgct tctacaactt ctggaaatac gcaggttgtc gctgcattat caaaataaat
     1921 catgtcgctt tctccctcta ttcttctatc gtaaacatca aaatagataa aactgtcatt
     1981 cctgctgtct cagttcttaa gatacgcttt ccaaggctca ttgtctgacc acctgctgcc
     2041 ttaagctgtt cgatctcttc cttttcaaaa ccgccttctg gtccgatgat gatcccgatc
     2101 gatttcttcc cttttgcctg actgatcagt tctttggatg cctgcattcc ttttgcctct
     2161 tcatatggaa caagcatgaa ttctatatct tgtacttctt ctaaaatatc tttgaaattt
     2221 ctcacttcac gaacttctgg aatgatccct cgttttgcct gttttgctgc gcttaatgcg
     2281 atcgtattcc aacgacttac cttctttgct gccttcttcg catcaagttt cacaacacaa
     2341 cgtttcatgg caactggaac gatctctgcc actccaagtt ccaccgcttt ttgaacgatc
     2401 atctccatct tatcgccttt tggcagtccc tgatacagcg tgatctttac tgggagttct
     2461 gtttccatgc catgaacatc ttcgatcttc gcaaggatcg tatcgtccgt ccattccaaa
     2521 aggctgcatt catattctaa atctgatccg ttacttaaat aaactttatc tcctgttttc
     2581 atgcggagaa cgttcttaat atgattgaca tcacttccac aaatctcgat cattccgtca
     2641 tgaatctgtt cttcttttat aaaaaaacgg tacatctctt acctcatctt cgctgtcata
     2701 gaaacccagt ctttcattct tcttgtctca atgacatcaa atccatatgc atttaatttg
     2761 tctgcgattt ctttttcttt tgtatcaatg ataccagaga ttacaaaaac accatcttct
     2821 gccaaaaact gatctacgat ctcgatcagt ggtgagatga catctgctaa aatattagca
     2881 acaacaaccg gataacattt tgatcctaag gatgctgcaa actctttatc atctaaaaca
     2941 tttccctgat atacttcaaa ctgatctttg ctgatatggt ttacttcaaa gttctcctca
     3001 gaagctgaga ttgcatgtgg atcgatatct gtaagaaccg catgacctgc cccaagtttc
     3061 agtccgatga tcgacaaaat tccacttccg catccaacat ccaaaatctt tgtatcttcc
     3121 ttaatatatg gtttcattcc ttcaatacaa agttttgttg tctcatgaga acctgttcca
     3181 aatgctgttc ccggatcgat ctctacaaca atcatatctt ctgtctgatc ttctaatgtc
     3241 tcccatgttg gtttgatcac gatctgttca tccagacgga tcggtttaaa gaatttcttc
     3301 cagttattga cccagtcttc ttctttcgtc ttatcaaatg aaattgttcc agcaccgata
     3361 tccataaatc cacgaagtct ttcaagcatt gcctgaatct gcattgtaat gtgctctaaa
     3421 ttttcattct catcaaaata acagctgatc tttgcttttc catcgtctgg tgcgatatca
     3481 tctggcaata gatccacata catgactttc atctcttctt ctgtcaatga tacatggtct
     3541 tcaacttcca caccagttgc accaagttct gtaagttcat aactgatcat gtcttctgct
     3601 gatgtcaatg tatcaattgt aattttattc catcccatga cactgtattt cctttctctt
     3661 tctatcttat atttttattt cgtattcata acatatcaat taattagtgt atcatacaaa
     3721 aagctggaat acaagaaaaa agagaatccc gtcgtctttc ggaactctct ttcatcgcta
     3781 tcttcttaat acgcaaactg tatttatgat gaatttatgc ttctaagatc tttccatcaa
     3841 tggaaattgt gtgaacctgc catggaagga atttgccgga atcctgaatc gctttcttcg
     3901 ccgcattttc cagcccgcca caacaaggaa cttccattct aacgatcgtt acactcttaa
     3961 tgtcattgtt cgcaatgatc tgtgtcagtt tctcactgta atctccttca tcaagttttg
     4021 gacagccgat caatgtcact ttatttttga taaatttctg atgaaaattt ccataagcat
     4081 aagctgtaca atctgccgcg atcaatagat tcgcatcctt aaaatatggg gcattaacag
     4141 gtactaattt aatctgcacc ggccactgag acagctgaga agatacagtt gctgtctgct
     4201 ctgtcgctac ctcttctgcc ttatgatcca tgatcttcat tcttgttcca gggcacccgt
     4261 ggaataccgg cttttcttct attttatctt ttgctgtcat ttcagctttc ttcttcatgt
     4321 ttgcttctac cgcttctctg tcatacgcag ctgcctcacg ctctacgaaa ctgatcgctc
     4381 ctgttggaca atttggcaga cagtctccta agccgtcaca ataatcatct cttaataatt
     4441 ttgcctttcc atcaactatc ccgatcgcat cttcgtgaca tgcatctgca cacagtccac
     4501 atccattaca tttatcttca tcaatttcaa taattctacg aatcatatct attctccttt
     4561 tcttgatctc atattcttta ctgtatcgtt agtataatag atatgatttg ggaaatccgt
     4621 tgtttttaca acatttttaa aattttttta aaaattgaaa aagcagaagg cactccataa
     4681 accttctgct ctttcggttg tttattattc gaatatgcca acaaagacat gttcttcctg
     4741 actatccgac gaaacaacac tccatattct cttcgtaaaa tctgttatac atctgtcgca
     4801 tcaaaggaat cagtttataa agtggttccg tcttacgatg gttaacttca tctttgatca
     4861 tctctttgat gatcgcttct tttacctctc ttagagtacc atatactcca actttttcca
     4921 atactttttt gcaattttct ttattctttg cgcgttttag taatacaaaa tctccataat
     4981 atttttttat ataataattt tctttcattc ttatcatttt aaagttctcc ttttcacata
     5041 ctgtgttt
//
LOCUS       DAOMUE010000136         4953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4734_length_4953_cov_2.758473, whole genome shotgun sequence.
ACCESSION   DAOMUE010000136 DAOMUE010000000
VERSION     DAOMUE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4734_length_4953_cov_2.758473"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(323..1159)
                     /locus_tag="PLC71_06880"
     CDS             complement(323..1159)
                     /locus_tag="PLC71_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM02401.1"
                     /translation="MSFHIVCDSCTDLTEEDLKKGCYTLVPLTLLVDGEEIIDDETFD
                     QADFLAKVAASKESVKSACPAPESYMEAYSKADDIYVVTLSAELSGSYNSAVLGKNLY
                     EEENGTKNIHVVNSRGAATTQVLIARKLNEYASQGMPFEEVVDKIEEYTTSLRTYFVL
                     ETLEVLRKNGRLSRLSATIAGALNIKPVMIGTRDGVIQKAAQARGMKKALAKMVEHMG
                     SEGRDLTRRQFVISHCNCYERAVYVKELIKKHLHAEDIDIVDTKGVSSLYACDGGIIV
                     SY"
     gene            complement(1284..1766)
                     /locus_tag="PLC71_06885"
     CDS             complement(1284..1766)
                     /locus_tag="PLC71_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5721 family protein"
                     /protein_id="HRM02402.1"
                     /translation="MVMIEIENIKKFMAGFLGGNLFDDFLMSEGKLSMNISYEFDGKI
                     LKEFYDTEEWKEMKQYPYVTWEEEKEKIFSLVKGKKTPLSFQFVMMLKPDLSPEFLAK
                     YNLAVREDEIAGLFINILYDRQGLKCTSGVSRKTFVLDKTLEEAWDQEVKERFLTKYL
                     "
     gene            complement(1858..3225)
                     /locus_tag="PLC71_06890"
     CDS             complement(1858..3225)
                     /locus_tag="PLC71_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRM02403.1"
                     /translation="MSEYNTNQDGQWKTSEQWRAEEANDHSNPKKNKNGGRGKYVAKL
                     AASAVAFGLIAGLVMQGVTYGFSRAGIGNGATQLATTKTTSSSSSTSSNDLSGVSKNV
                     MPSIVSITGKFETTSQSWFGQTQSSESEGVGSGIIIGKKDGKILIVTNNHVVADAKSL
                     SVGFVDGKSASATIRGTDSDADLAVVEVDTKNMKSSTLKKIAVITLGDSSKLQTGERA
                     IAIGNALGYGQSVTGGYISALNRQVQLTDKTMTLIQTDAAINPGNSGGALLNSKGELI
                     GINTVKYSSEDVEGMGYAIPINTAKPIINKLIKNEATSESEQAYLGVSGQTIDSSMAS
                     QFDMPSGVYVQQVIKSSPAQKAGISAGDVIVSFNGSNVSTMDGLKEKISNLKAGKKVK
                     VVVKRQNQMGTYEKKTLTVTLGKKSDAPSTSSNSSNSNSNNSNSYNNNYNNESSNSQY
                     YSFGQ"
     gene            3559..>4727
                     /locus_tag="PLC71_06895"
     CDS             3559..>4727
                     /locus_tag="PLC71_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HRM02404.1"
                     /translation="MYFAGFAAWYIRFKCTIFGFWLNQEIFDLNRYYPEFYQYQKPLI
                     SSLILLLLLYSFFGLYTPKRYQRGSKELVNLVKANLIGLGLSAFVITVWQIQNFPRSL
                     YLLFYLFNFIFGLLSRYIIRRILKTNRKKGRNIKHTVFIGFSTSAAAYIDRIKSNPQW
                     GLKVHGIFDDLVSDNFEYRGIKKIGTLSDLAAYLEKTSLDEVAITLNLNEYHKLEQIV
                     AICEKSGVHTKFVPDYYNFISTNPYTEDLDGLPVINIRNVPLTNTMNKLIKRLIDIIG
                     SIIALILFSPIMLIVAILVKKSSPGPIIFAQERIGLGNKPFKMYKFRSMGVQDPKKEA
                     KEWTTKNDVRVTPVGRVIRKTSLDELPQFWNVLKGDMSLIGPRPERPLFVEKFKE"
     assembly_gap    4728..4737
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4738..4935
                     /locus_tag="PLC71_06900"
     CDS             <4738..4935
                     /locus_tag="PLC71_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRM02405.1"
                     /translation="PRYMIKHQVRPGITGWAQVNGFRGDTSIRSRIEHDLYYIENWSL
                     GLDIKILFLTFFKGFVNKNAY"
BASE COUNT         1339 a         1035 c          805 g         1764 t
ORIGIN      
        1 atacgcacat ttttcgacaa attcaagtgt ttttttgtgc aatttgtacg ttaatttcgt
       61 aacaatccag ctcatttttg tgcataatca cgttaatttt gtaacaattt taagttgtag
      121 ttagccaact atagattttg aatatatgat aaaaaataaa taaacgatcg ggagagcaaa
      181 attctaatgc tgctctcccg atcgtttatt tattttttat catatatcag aagtctatcg
      241 tgtagacaat tataaacttg gaaaatttaa gtggatgttt atttctatca cttatataca
      301 cgtctattca agtaaccaga atctaatatg atacaatgat tcctccatcg catgcataca
      361 aactgctgac tcctttagta tctacaatat caatgtcttc tgcgtgtaaa tgcttcttga
      421 tcaattcttt cacgtaaaca gctctctcgt agcaattgca gtgtgaaatg acgaattgtc
      481 ttcttgtgag atctcttcct tcgcttccca tgtgttctac catcttcgcg agtgctttct
      541 tcattcctct tgcctgggct gctttctgaa tcacgccgtc tcttgtaccg atcatgactg
      601 gtttgatatt taatgcaccg gcgatcgttg cagatagtct tgataatctt ccgttctttc
      661 ttagtacttc tagtgtctcc aaaacaaaat atgttctaag acttgttgta tactcttcaa
      721 tcttatcaac cacttcttca aatggcattc cctgacttgc atattcgttg agcttacgtg
      781 cgatcagaac ctgagtcgta gcggctcctc tggaattcac tacatgaata ttctttgttc
      841 cgttctcttc ttcatataga ttctttccta aaactgcact gttgtatgat ccacttaatt
      901 ctgcagataa tgttaccaca tatatatcat ctgccttgga atatgcttcc atataactct
      961 ctggtgcagg gcatgctgac ttcacagact ctttacttgc tgcaaccttt gctaaaaaat
     1021 ctgcctgatc aaatgtttca tcatctatga tttcttctcc atccactaat aatgtcaggg
     1081 gtactaaagt ataacatcct ttttttaaat cttcttctgt caaatccgta cagctgtcac
     1141 aaacaatatg aaaactcata ttcgtatctc ctttttacaa ctttcttatc tggtattcat
     1201 tactacttat tctaacatat atctacgcaa aaagaaacat gcaaacaaag tttcttttaa
     1261 gttcccgaag tttgtgaaaa attttacagg tactttgtta aaaatctttc tttcacttcc
     1321 tgatcccacg cttcttccaa agtcttatcc agtacaaatg tctttctgga cactcctgat
     1381 gtacacttta atccctgtcg gtcgtataaa atgttgataa ataatcctgc tatttcatct
     1441 tctcggactg caagattata tttggcaaga aactctggag aaagatctgg ttttaacatc
     1501 atcacaaatt gaaaagacag cggtgtcttc ttccctttta cgagagaaaa tattttttct
     1561 ttctcttctt cccacgttac atatggatat tgtttcatct ctttccattc ttctgtatca
     1621 taaaattctt ttaagatctt tccatcaaat tcatacgaaa tgttcatgga cagttttcct
     1681 tcgctcatca agaaatcatc aaaaagattt cctcctaaaa aacctgccat aaacttctta
     1741 atattttcaa tttcgatcat aaccataggc atattctcct gactaaaaaa tggaaccctg
     1801 cctactcaca gggttccatt ttattgtatc tttctttggt tcttttttca accggtttta
     1861 ctgtccaaaa ctataatact ggctgttaga actttcatta ttataattat tgttgtaact
     1921 attgctgtta ttactattgc tgttgctgct gttactgctt gtgcttggtg catctgattt
     1981 ctttccaaga gtaactgtca gtgtcttctt ctcataagta cccatctgat tctgacgttt
     2041 tacaacaact ttaactttct tgcctgcttt caggtttgaa atcttttctt ttaatccatc
     2101 cattgtagaa acgttgcttc cattaaagga tactatcaca tctcctgcac tgatgcctgc
     2161 tttctgggct ggggaactct tgatcacctg ctgtacataa acaccagatg gcatatcaaa
     2221 ctggcttgcc atagagctgt cgatcgtctg acctgataca cctaaatatg cctgttcact
     2281 ctcacttgtt gcctcattct tgatcagttt attgatgatc ggttttgctg tattgatcgg
     2341 aatcgcatat cccattcctt ctacatcttc tgaagaatat ttaactgtat taataccaat
     2401 taattcacct ttgctattta ataaagcacc accactattt ccagggttga tcgctgcatc
     2461 tgtctgaatc agtgtcattg tcttgtctgt caactgtacc tgacgattta atgcactgat
     2521 ataaccacct gtgacagact gtccatatcc taatgcatta ccaattgcga tcgctctttc
     2581 tcctgtctgt aattttgagg aatctcctaa tgtgatcact gcgatcttct ttaaggtaga
     2641 agatttcatg ttctttgtat ctacttctac aacggctaag tccgcatcgc tgtcagtacc
     2701 acgaatggtt gcacttgcac tctttccatc cacaaatcct acggataaag atttggcatc
     2761 tgctacgaca tggttatttg taacgatcaa aatcttacca tctttcttgc caatgatgat
     2821 acctgaacca acaccttcgg attcactgct ttgtgtctgt ccaaaccaac tctggcttgt
     2881 tgtctcgaac tttcctgtga tcgatacgat cgatggcata acattttttg atacacctga
     2941 gaggtcatta ctgctggtac ttgaactgct gcttgttgtc ttcgttgttg caagctgtgt
     3001 tgcaccattt ccaatgcctg ctctgctaaa tccatatgtg actccctgca tcacaagccc
     3061 tgcgatcaat ccaaaagcta ctgcagatgc tgctaatttt gcaacatatt ttcctctgcc
     3121 gccatttttg tttttcttag gattggaatg gtcgttcgct tcctccgctc tccactgctc
     3181 ggaagtcttc cattgtccat cctgatttgt attatattca ctcatgattt ttattctcct
     3241 ttcgatcctg atcatttgtt ttgatctttt tctcttgatt tcgtcctttt gcttttattt
     3301 tctcatgtgt ttttgccttt catgagataa atatatctta taaatgtgta aaaactgtga
     3361 ttaatatgtt actaaaaaat gatttcctct tttttctcat ttttcgtgaa aatgatgaga
     3421 gtgtcaataa gtacaaaata caaaaataag aaaaagttaa gtcatttgtc attgtatgat
     3481 ctctttttat cccttataat agaaggaagt gaaaaaggag tatttatcat atgatcaaac
     3541 aaaaccaaac ttgtctgcat ttattttgca ggatttgcag catggtatat aagatttaaa
     3601 tgcacgatct ttggattctg gctgaatcag gagattttcg atctcaaccg ctattatcca
     3661 gagttttatc aatatcagaa accgctgatc agctctttga tcctgttatt gttattgtat
     3721 tcgttctttg gtctttatac accaaagcgt taccagcgag gttccaagga acttgtgaat
     3781 ctggtcaaag cgaatctgat cggacttgga ctttctgcat ttgtgatcac tgtatggcag
     3841 attcagaact ttcctcgttc tttatatctg ttattttatt tatttaactt tatttttggt
     3901 cttttatcca gatatatcat acgcaggatt ttgaaaacga atcgtaagaa aggacgcaat
     3961 atcaaacata cggtatttat cggtttcagt acttcagcag ctgcatatat cgaccgcatc
     4021 aaatcaaatc ctcaatgggg cttaaaggtt catgggattt ttgatgatct tgtcagtgat
     4081 aattttgaat atcgtggaat caagaagatt ggtacacttt cagatcttgc cgcttatctt
     4141 gagaagacat ctcttgatga agttgcgatc actctgaatc tgaatgaata ccataaactg
     4201 gaacagatcg ttgcaatctg tgagaagtct ggtgtgcata caaagtttgt tccagattac
     4261 tataacttta tttccacaaa cccatacact gaagatctgg acggactccc agttatcaat
     4321 atccgtaacg taccattaac aaatacgatg aataaactga tcaaacgtct gatcgatatt
     4381 atcggttcta tcattgcgct gatcttattc tcaccgatca tgcttatcgt tgcgatcctt
     4441 gtgaagaaga gttctcctgg accgatcatt tttgcccaag aacgtatcgg tcttgggaat
     4501 aaacctttta agatgtacaa attccgttcc atgggtgtac aggaccctaa gaaagaggcc
     4561 aaagaatgga caaccaagaa cgatgtccgt gttacacctg ttggacgtgt gatccgtaag
     4621 acaagccttg atgaacttcc tcaattctgg aatgtattaa aaggcgatat gagtctgatc
     4681 gggccacgtc cagaacgacc tttgtttgtc gagaaattta aagaagannn nnnnnnncct
     4741 cgttatatga tcaagcatca agttcgtcca ggtattactg gatgggctca ggtcaatgga
     4801 ttccgtggtg atacttccat ccgaagcaga atcgagcatg acctgtatta tattgagaat
     4861 tggtctttgg gacttgatat caagatatta tttcttacat tctttaaggg atttgtcaat
     4921 aaaaatgcat attaaacagc aagaaaagga tgg
//
LOCUS       DAOMUE010000137         4930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4758_length_4930_cov_3.228923, whole genome shotgun sequence.
ACCESSION   DAOMUE010000137 DAOMUE010000000
VERSION     DAOMUE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4758_length_4930_cov_3.228923"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1279)
                     /locus_tag="PLC71_06905"
     CDS             complement(77..1279)
                     /locus_tag="PLC71_06905"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRM02406.1"
                     /translation="MLVVKKFGGTSVGNKDRIMNVAKRCIEDYKKGNDVVVVLSAMGK
                     MTDELIAKAKEINPNPSRREMDMLLTTGEQTSVSLMAMAMDSLGVPAVSLNAFQVAMH
                     TTSVYSNARIKRIDTDRIMNELEQRKIVIVTGFQGVDQFDNYTTLGRGGSDTTAVALA
                     AALHADACEIYTDVDGVYTADPRKVSEAKKLNEITYDEMLEMATLGAGVLHNRSVELA
                     KKHGVALVVRSSLTTEEGTVVKESVKSRDNEISGVAGDDDAARIAVMGIEDTPGMAFK
                     IFNVLAKKNVNVDMILQSVGHNDKKDISFTVKEEELDIAVKALEDYLPDSYYEEIKAS
                     KEVAKVSIIGKGLLNHYGMAARMFNALYKADVNIRMISTSEIRVTVLVNQEDMVKAMN
                     AAHEEFEM"
     gene            complement(1305..2504)
                     /locus_tag="PLC71_06910"
     CDS             complement(1305..2504)
                     /locus_tag="PLC71_06910"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HRM02407.1"
                     /translation="MKYVIVLGDGMADEPIEAAGGKTPLQLADKPVMDEMAKVSEQGI
                     AYTVPSNLPAGSDVANLSMLGYDPQVYYSGRSPLEALSIGVDMKDTDIALRCNVVTVS
                     EDEDYEDKTIIDHSAGEITSEEAAILIEAVRKELEDDEYKFYPGVSYRHLTIWDHGEV
                     VELVPPHDVLGQKIGQYLPKQEKLKEMMEKSFDILNHHPINEARAEKGLNKANSIWFW
                     GAGTKPALDDFKEKTGMNGAMVSAVDLLKGIAVGANMRNLDVEGANGGLDTNYHGKAM
                     AAVEALLEKNDDFAYIHVEAPDEMGHQGSLENKIKAIEHLDKEVVKVVKEELEKAGED
                     FRILIGPDHPTPIAIRTHSKEPIPFMIYDSTKDYEGQEEYSEEAARETGIVISHAYDL
                     MNRLLQK"
     gene            complement(2542..2982)
                     /locus_tag="PLC71_06915"
     CDS             complement(2542..2982)
                     /locus_tag="PLC71_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRM02408.1"
                     /translation="MKETNYYVIKKKAVPEVLLKVLEVQKLLDSKRAVSVLEATEKVG
                     ISRSSYYKYKDDIFPFYAGKKGRSITFVIEVDDQPGVMASILNIFALYKANILTIHQS
                     IPINGKGLLTVSVDIEDSETDVSAMIQDVEKIEDVSYVKIIAME"
     gene            complement(3111..4232)
                     /gene="prfB"
                     /locus_tag="PLC71_06920"
     CDS             complement(3111..4232)
                     /gene="prfB"
                     /locus_tag="PLC71_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRM02409.1"
                     /translation="MIEAEQLKYKLNSFQEPLEDLSGSLALEAKKERIDQLELNMEEP
                     GFWDNVEESQNVMKEVKSLKGVVEEYDDLKTKYEDIETLIDMAEEDEDADLIEEATAL
                     MDEFEKTYEELRISTLLTGEYDKDDAILTLHAGAGGTESCDWAGMLYRMFTRWAGKKG
                     YGTEVLDYLDGDEAGIKSVTVKITGMNAYGYLRSEKGVHRLVRISPFNAAGKRQTSFA
                     SCDVMPDIKDDIEVEIADEDIRIDTYRASGAGGQHINKTDSAIRITHLPTGIVVQCQN
                     ERSQHKNKDQAMKMLKTKLYLLKQQQHLEKLSDIRGDVGDNGWGNQIRSYVLQPYTMV
                     KDHRTNFESGNPSAVLDGDLDGFMNAYLAWDNNSKKEEN"
     gene            complement(4247..>4930)
                     /locus_tag="PLC71_06925"
     CDS             complement(4247..>4930)
                     /locus_tag="PLC71_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRM02410.1"
                     /translation="MYKERRRVLDGDDMKESVLGMMKETVANHVYQVISDELPPEEWD
                     LAALNAELLPIVPLNKIVLTDAEKSSGKVDDLVARLQEETVALYEQKEKEFEDFDLRE
                     IERIILLKVIDRKWMDHIDDMDQLREGIGLQAYGQRDPVVEYRMAGFEMFNDMTKAIQ
                     EETTGALFHVQVEQKIEREEAPKITGTNKDAEVEKKPYVRKGAKVGRNDPCPCGSGKK
                     YKNCCGRNK"
BASE COUNT         1216 a         1169 c          771 g         1774 t
ORIGIN      
        1 gctgctcttc cgatcgttta tttatttttt atcatatact tgaaatctac agttggacaa
       61 cttacaaaat taaattttac atctcaaatt cttcatgtgc tgcattcatt gctttcacca
      121 tatcttcctg gtttacaagc actgtaacac ggatctcaga tgtagagatc attcgaatgt
      181 ttacatctgc tttatataaa gcgttaaaca tacgagctgc cattccatag tggtttaata
      241 atccttttcc gataattgat actttggcaa cttctttgct tgctttgatc tcttcataat
      301 agctgtctgg aaggtaatct tctaatgctt taactgcgat atctaattct tcttctttga
      361 ctgtgaagga gatatctttc ttgtcattat gtccaactga ctgcaagatc atatctacgt
      421 ttacattttt ctttgctaat acattgaaga tcttgaatgc catacctggt gtatcttcga
      481 ttcccataac tgcgattctt gctgcatcat catctcctgc tacaccgctg atctcgttgt
      541 ctcttgattt tacgctttct tttacgacag ttccttcttc tgttgtaaga cttgatctaa
      601 caactaaggc aactccatgt ttcttagcaa gttctacaga acgattatga agtactcctg
      661 ctccgagtgt tgccatttct aacatctcat catatgtgat ctcatttaat tttttagcct
      721 ctgatacttt tcttgggtct gctgtataga ctccgtctac gtctgtataa atctcacatg
      781 catctgcgtg taacgctgct gccagtgcta cagctgttgt atcagatcca ccacgtccaa
      841 gtgttgtgta gttatcgaac tggtcaactc cctggaatcc tgtaacgatt acgatctttc
      901 tctgttctaa ttcattcatg atacggtctg tatcgattct tttgattctt gcattactgt
      961 atacagaagt tgtatgcatt gctacctgga atgcatttag ggaaacagca ggaactccaa
     1021 gagaatccat tgccattgcc attaatgata cagaagtctg ttctcctgtt gttaataaca
     1081 tatccatctc tcttctggat ggattaggat tgatctcttt tgcttttgcg atcagttcat
     1141 ctgtcatctt acccattgct gataagacaa caacaacatc atttcctttt ttgtaatctt
     1201 caatacatct ttttgcaacg ttcatgatac gatctttatt tcctacagaa gtaccaccaa
     1261 atttcttaac tactagcatc ttgtgcctcc taaaattatt tctcttattt ttgtaataac
     1321 cgattcatta aatcataagc gtgggaaatg acaattcctg tctctctcgc tgcttcttcg
     1381 ctgtactctt cctgaccttc ataatctttc gtgctgtcgt agatcataaa tggtatcggt
     1441 tctttggaat gggtacggat cgcaattggt gtcggatgat ccggtccgat cagaattcta
     1501 aaatcctctc cagctttctc aagttcttct ttgacaacct tcacaacttc tttatcaaga
     1561 tgttcgattg ctttgatctt attctcaaga cttccctgat gacccatctc atcaggtgcc
     1621 tctacatgaa tataagcgaa atcatcattt ttttcaagta gtgcttcaac tgctgccatc
     1681 gctttcccat gataattcgt gtcaagaccg ccattggcac cctctacatc aagatttctc
     1741 atgtttgctc caactgcgat tccttttaac agatcgacgg cagataccat agctccattc
     1801 attccagtct tctctttaaa atcgtccagt gccggtttcg tccctgctcc ccagaaccag
     1861 atgctgtttg ctttattcaa gcctttctca gctcttgctt cattgatcgg atgatgattt
     1921 aagatatcaa aactcttttc catcatctct tttagcttct cctgctttgg aagatactgc
     1981 ccgatcttct gtcctaaaac atcatgaggt ggtacaagtt cgactacttc tccatgatcc
     2041 cagatcgtta aatggcggta gctgactccc ggataaaatt tatattcatc atcttctaat
     2101 tcttttctca cagcttcgat caggatcgct gcttcctcag aagtgatctc tcctgcgctg
     2161 tggtcgatga tcgtcttatc ttcataatct tcgtcttcag atacggttac aacgttacat
     2221 ctaagtgcaa tatctgtatc tttcatatca acaccaatgc ttaatgcctc aaggggtgat
     2281 cttccggaat aataaacctg tgggtcatat cctaacatag aaagattggc tacatcactt
     2341 cctgctggta aattggatgg tactgtataa gcgattccct gttcggaaac tttcgccatc
     2401 tcatccatca ctggtttgtc tgctaactga agtggtgtct tcccacctgc tgcttcgatt
     2461 ggctcgtcag ccattccatc tcctaaaaca ataacgtatt tcattctgtc ctccaaaaat
     2521 gtgtcatgta tatttataca tctattccat tgcaattatt tttacataag atacatcttc
     2581 tatcttctca acatcctgaa tcatagccga gacatccgtc tcgctgtctt cgatgtcaac
     2641 actgactgtt aagagtccct ttccattaat tggaatactc tggtggatcg tcaggatatt
     2701 tgccttataa agtgcaaaaa tgttaaggat cgaggccata acgccaggct ggtcatcgac
     2761 ttcgatcacg aatgtgatac tacgtccttt cttaccagca taaaatggga aaatatcatc
     2821 cttatacttg taataagaac ttcgactgat tcccaccttc tctgtagctt ctaatacaga
     2881 aactgctctc tttgaatcta acagtttctg gacctctaac acctttaaca acacttctgg
     2941 aactgctttc tttttaatta cataatagtt ggtttctttc atgtctggtt ccttttgttt
     3001 tcctgtggtg tacagttgtc tttgcacaaa atactaccac agatattttt atgatgcaat
     3061 agaaaatttg gttttaaaat aaatacatga tctgaccgga agttttctgt ttaattttct
     3121 tccttcttac tgttgttatc ccatgcaaga taggcattca taaatccatc cagatcccca
     3181 tctaatacag cacttggatt gccactttca aagtttgttc tgtggtcttt taccattgta
     3241 taaggctgca ggacatagga tctgatctgg tttccccatc cattatcccc aacatcacct
     3301 cggatatctg ataatttttc caaatgttgc tgctgtttta acagatacag ttttgtcttt
     3361 aacatcttca ttgcctgatc tttgttcttg tgctgtgaac gctcattctg gcactgtaca
     3421 acgattcctg ttggtaaatg cgtaatacgg attgcggaat ctgttttgtt gatatgctgt
     3481 ccaccagctc cacttgcacg atatgtgtcg atccggatat cttcatctgc aatctcaact
     3541 tctatatcat ctttgatatc tggcattaca tcacaggatg caaacgatgt ctgtcgtttt
     3601 cctgctgcat tgaatggaga aattcggaca agacggtgaa cacctttttc agatcgtaaa
     3661 tatccataag cgttcattcc cgtgatcttc acagtaacag atttgattcc tgcttcatcc
     3721 ccatcaagat aatcaagaac ttctgttcca taacctttct ttcctgccca tcttgtgaac
     3781 atacggtata gcattcctgc ccagtcgcaa gactctgttc cacctgcccc ggcatgtagt
     3841 gtaagaattg catcatcctt atcatattca cctgttagca gtgttgaaat acgtaattcc
     3901 tcatatgttt tttcaaattc atccatcaaa gcagtggctt cttcgatcag atcggcatct
     3961 tcatcttctt ctgccatatc gatcaaagtc tcgatatctt catattttgt tttcagatca
     4021 tcatattctt ccacgacacc tttcaggctt ttgacttctt tcataacatt ctgggattcc
     4081 tcaacattat cccagaatcc tggttcttcc atattcagtt ctaattgatc aattctctct
     4141 ttttttgctt ctaacgcaag ggaaccacta agatcttcta gtggttcctg aaaagagttt
     4201 aatttatatt ttaattgttc tgcttcaatc attgataaaa aactccttat ttattccttc
     4261 cgcagcagtt cttatacttc ttaccacttc cacatgggca aggatcattt ctgccgactt
     4321 ttgctccttt tcttacataa ggcttcttct caacttctgc atctttgttt gttccagtga
     4381 tctttggagc ttcttctctc tcgatcttct gctcaacctg tacgtggaac agtgcacctg
     4441 ttgtctcttc ctggattgcc tttgtcatat cattgaacat ctcaaatcca gccatacggt
     4501 attcaacaac tggatctctc tgtccatatg cctgaagacc aattccttct cttaattgat
     4561 ccatatcatc aatatgatcc atccacttgc ggtcgatgac tttcagtaaa ataatacgtt
     4621 caatctcacg aagatcaaag tcttcgaatt ctttttcttt ctgttcatat aatgcaactg
     4681 tttcttcctg taatcttgct accagatcat caactttacc agaagatttc tctgcatctg
     4741 ttaatacgat cttattcagt ggtacaattg gaagcaactc tgcattcagt gctgccagat
     4801 cccactcttc tggtggaagc tcatcactga tcacctgata tacatgattt gcaactgttt
     4861 ctttcatcat gcctaaaaca gattctttca tgtcatctcc gtcaagaaca cggcggcgtt
     4921 ctttgtacat
//
LOCUS       DAOMUE010000138         4889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4824_length_4889_cov_2.636119, whole genome shotgun sequence.
ACCESSION   DAOMUE010000138 DAOMUE010000000
VERSION     DAOMUE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4824_length_4889_cov_2.636119"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>208)
                     /locus_tag="PLC71_06930"
     CDS             complement(<1..>208)
                     /locus_tag="PLC71_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRM02411.1"
                     /translation="LEKAGYKDGDKIKIEYKNAQNEQSNCQTIAKQFVTDKCDLVLAI
                     ATPAAQAMANESKDIPILVTAVTDP"
     assembly_gap    209..218
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<219..385)
                     /locus_tag="PLC71_06935"
     CDS             complement(<219..385)
                     /locus_tag="PLC71_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HRM02412.1"
                     /translation="MRLKKVMATGLVAALALSTMVGCSSKKDSSDQKKIGVVQLVEHD
                     ALDASYKGFKDG"
     gene            complement(645..2078)
                     /gene="malQ"
                     /locus_tag="PLC71_06940"
     CDS             complement(645..2078)
                     /gene="malQ"
                     /locus_tag="PLC71_06940"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRM02413.1"
                     /translation="MKTSGILLPIFSLPSDYGIGCFSKEAYKFVDFLEEAGQKYWQIL
                     PLGTIGAGNSPYLSFSSFAGSAYYIDLEQLLEEGLLKKKELESIKEENKGKVDYEKIR
                     EDHRKLLRKAYFRFHPDEDYKKFIKDNEYWIGEYAEYMSEKKSKFPESYFCFCQYYFH
                     KQWLALKKYANDKGIQIVGDLPFYVALDGTAFTYHKELFKVDEEGKATVVGGCPPDAF
                     AEDGQVWSNPVYDWEYHKKTDYEWWMKRLRHNFMLYDILRLDHFRGFDEYFEIPAEDE
                     TAVNGEWVKGPGMDFFEAMKKELGEKKVIAENLGFLTDSVHKLLDDTGFPGMNVLEFA
                     FGAYDDSIYLPHKYKENSVVYTGTHDNDTVVGWYETLSEDDKKFLEHYLKYSTIERTG
                     TVHLDLISLALESRSDMVIIPLQDYLGLGNEARINTPSTVGGNWEWRVKEEQLTDELK
                     ELIRTLTIKYRTVAEENAKKAAEEAQQ"
     gene            complement(2189..3343)
                     /locus_tag="PLC71_06945"
     CDS             complement(2189..3343)
                     /locus_tag="PLC71_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HRM02414.1"
                     /translation="MDYNKLALEMHEQNKGKIAVRSKVTVKTRDDLSTAYTPGVAEPC
                     RKIRDNKEEVYRYTAKGNLVAVVSDGTAVLGLGDIGPEAAMPVMEGKALLFKEFADID
                     AFPICLDTKDTEEIIKTVKNIAPCFGGINLEDISAPRCFEIEKRLKEELDIPVFHDDQ
                     HGTAIVVAAGLLNALKFVGKKMEDANIVINGAGSAGISICKLLLQFGAGNVVLVDQKG
                     ALCPGEDWMNPAQKDMAEITNKEKQTGTLTEIIKDKDVFIGVSAPNIVTAEMVSTMAD
                     DAIIFAMANPTPEIMPDEAKKGGARVIATGRSDFPNQINNVLVFPGIFRGALDARAGQ
                     ITEEMKMAAARAIASIISDDELNEEYIIPGAFDERVCKAVAKAVAEESQK"
     gene            complement(3474..4427)
                     /locus_tag="PLC71_06950"
     CDS             complement(3474..4427)
                     /locus_tag="PLC71_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRM02415.1"
                     /translation="MFQLAENRQEAGRELRDCVVETLPELMKDDDKITALEADLGGAS
                     GFTKIKKTNPERFIQCGIAEANMMGVAAGLSLTGFKPFTHTFAPFATRRVFDQLFLSG
                     AYAGNTINVYGSDPGFSVASNGGTHTAWEDVALIREIPGAVICDPADDVQMEWIIKEF
                     LKMEGIHYVRSNRKAVRNVYKKGSSFKIGQGNILKEGKDILIIAAGQLVSEALDCAEE
                     LEKEGYSVEVIDMFTIKPLDEKLLIKEAKGKSKIVTIENHSIYGGLGSAVSEVIAENG
                     ISVPVKRIGVKEKFGQVGTAEFLQEEFGLTAKQIKETICQF"
     gene            complement(4429..>4889)
                     /locus_tag="PLC71_06955"
     CDS             complement(4429..>4889)
                     /locus_tag="PLC71_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HRM02416.1"
                     /translation="GFKMNKTDQYVYLIVGDGELNEGQCWEAFQYIAHYKLNHCVVII
                     DDNKKQLDGTTKEVMNPFSIENKMKAFGFYTQTVKGNDEEAIDEAINRAKNVKDQAVC
                     IILDSIKGAGVPYFEQLDANHSVKFNNDTIKKATKDAIKELDEFIKGGAR"
BASE COUNT         1268 a         1096 c          693 g         1822 t
ORIGIN      
        1 gggtctgtaa cggctgttac tagaattgga atatctttgg attcatttgc cattgcctgt
       61 gctgctggtg ttgcgatcgc taatacaaga tcgcacttat ctgtgacaaa ttgtttggca
      121 attgtctggc aatttgactg ttcattctgt gcgttcttat attcaatctt gatcttgtct
      181 ccgtctttat atccagcttt ctcaagacnn nnnnnnnncc atccttaaac cctttgtaag
      241 atgcatctaa agcatcatgt tctactaact gaacgactcc gatcttcttc tggtctgaac
      301 tgtctttctt gcttgagcaa ccaaccattg tggataatgc taatgcagca actaatcctg
      361 ttgccatgac tttctttaat ctcatatttt tctcctcctt aatatcgttc ccgattgcat
      421 tccttggctt taaacttctg aactttaaaa cttcaatgct tttatgtact aaattgtagc
      481 actatgattt tgtgtcgtca agctaatttt attgctttat atggtacatc atattccata
      541 caatctaata tcaattattt tatacgcata tttctttatt tctatgcaaa aaaggatgaa
      601 agatttccca aattctttca tcctttttct tctatcaatt tcttctattg ctgtgcttct
      661 tcggctgctt tcttcgcatt ctcttctgca actgtacgat attttattgt cagtgttctg
      721 atcagttctt ttaactcatc tgttaactgc tcttctttca ctctccactc ccagttacca
      781 cctaccgtag atggtgtgtt gatccttgct tcatttccta atccaagata atcctgaagt
      841 gggatgatca ccatatcgct tcgactctct aatgcaagag aaatcaagtc taaatgtaca
      901 gttcctgttc tctcgatcgt actgtatttt aaataatgct ctaagaattt cttatcatct
      961 tctgataatg tttcgtacca tccaacaacc gtatcattgt catgtgttcc tgtgtataca
     1021 acactatttt ctttgtattt atgtggaaga tagatgctgt catcatatgc tccaaatgca
     1081 aattccagaa cattcattcc tgggaatcct gtatcatcta gaagtttatg cacgctgtct
     1141 gttaagaatc ctaagttctc tgcgataact ttcttctcac caagttcttt cttcattgct
     1201 tcaaagaaat ccattcctgg acctttgacc cattctccat taacagctgt ctcgtcttct
     1261 gctggaatct caaagtattc atcgaatcct ctgaaatggt ctaatcttaa aatatcatat
     1321 aacataaaat tatgacgtaa acgtttcatc caccattcgt aatctgtttt cttatgatat
     1381 tcccagtcat atacaggatt ggaccatacc tgtccatctt ctgcaaatgc atctggagga
     1441 catccaccta caaccgttgc ctttccttct tcatcgactt taaataattc tttgtggtat
     1501 gtaaatgctg ttccatctaa ggctacataa aatggaagat ctcctacgat ctgaattcct
     1561 ttgtcattcg catatttctt cagcgcaagc cactgtttat ggaaataata ctggcagaag
     1621 cagaaataac tttctgggaa tttacttttc ttctcgctca tatattctgc atattccccg
     1681 atccagtatt cattgtcttt gataaatttc ttataatctt catctggatg aaatctgaaa
     1741 taagcttttc ttaaaagctt tctgtgatct tctctgattt tctcatagtc aactttgccc
     1801 ttgttttctt ccttaattga ttcgagttct tttttcttaa gaagtccttc ttccagtaac
     1861 tgttctaagt cgatataata tgcacttcct gcaaaacttg aaaacgaaag ataaggagaa
     1921 tttcccgcac cgatcgttcc taatggaagg atctgccagt acttctggcc tgcttcttct
     1981 aaaaaatcta caaatttgta tgcttccttt gaaaagcatc caattccgta atctgatggt
     2041 aaactaaaaa ttggcaataa tattccgctt gttttcattg aatgaattct cctaaaatct
     2101 cctaaaattt tctcacaact caatatagta tatcataaaa catacttctt gaacagaagt
     2161 gctcttgtac ttaatttaat tctatcattt atttctggct ttcttctgca acagctttgg
     2221 ctactgcttt gcagactctt tcatcaaatg cacctggaat gatatactct tcatttaact
     2281 catcatcact gatgatactt gcgatcgctc ttgctgctgc catcttcatc tcttctgtga
     2341 tctgtccagc tcttgcatcc agagctcctc tgaagattcc tgggaatact aatacgttat
     2401 tgatctgatt agggaaatcg gatcttcctg ttgcgataac tcttgctcca cctttcttcg
     2461 cttcatctgg catgatctct ggtgttggat ttgccatggc aaaaatgatc gcatcgtctg
     2521 ccatagtaga taccatttcg gctgtcacga tatttggtgc agaaactccg ataaatacgt
     2581 ctttatcttt gatgatctct gttaatgttc cagtctgttt ctctttgttt gtgatctctg
     2641 ccatatcttt ctgtgctgga ttcatccagt cttctcctgg acataatgca cctttctggt
     2701 caactaagac aacatttcct gcgccaaact gtaataatag tttacagata gagattccag
     2761 cagatcctgc tccattgatc acaatgtttg catcttccat tttctttccg acaaatttta
     2821 aggcatttaa taatcctgct gctacaacga tggctgttcc atgctgatca tcatggaata
     2881 ctggaatatc taattcttct tttaaacgtt tttcaatctc aaaacatctt ggtgcggaaa
     2941 tatcttctaa attgatacca ccgaaacatg gtgcgatatt cttgactgtt ttgatgatct
     3001 cttctgtatc ttttgtatca agacagattg ggaatgcatc aatatctgca aattctttaa
     3061 ataaaagggc ttttccttcc ataacaggca ttgctgcttc tgggccgata tctccaagtc
     3121 ctaatacagc tgttccgtca gatacgacag ctactaaatt accttttgct gtataacgat
     3181 atacttcttc tttattatca cggatcttac gacaaggttc tgcgactcct ggtgtataag
     3241 ctgtacttaa gtcatctctt gtctttactg ttactttaga acgtactgcg atcttacctt
     3301 tgttttgttc gtgcatttct aatgccagtt tgttgtaatc cattttttgt atctccttta
     3361 tgattcttca gattttgctt ctttaaacag tataccttat caaacaaagc gagtctagtc
     3421 cgaagagaca atcacaaaaa aggagcagaa cttttactct gctccttcac agcttaaaat
     3481 tgacaaattg tttcctttat ctgtttagcg gtcaatccaa attcctcttg taaaaattct
     3541 gctgttccta cttgtccaaa tttttctttg acaccaattc tttttactgg cacagaaatt
     3601 ccattttctg caatcacttc tgaaaccgca cttccaagtc cgccataaat tgaatgattt
     3661 tcaatggtta caattttgct tttccctttt gcttccttga tcaataattt ttcatccaaa
     3721 ggcttaattg tgaacatatc tataacttct acagaatatc cttctttttc taattcttca
     3781 gcacaatcca atgcctcaga aactagttgc ccagctgcaa taattaatat atcttttcct
     3841 tcttttaaaa tatttccctg tccaatttta aaagaggaac cttttttata tacatttcgt
     3901 acagcctttc gattactcct tacataatgg attccttcca ttttcaaaaa ctctttgatg
     3961 atccattcca tttgtacatc atctgctgga tcgcagataa ctgctcctgg gatttctctt
     4021 atcaatgcta catcttccca cgctgtatga gttccaccat tggatgccac actaaatcct
     4081 ggatctgagc cataaacatt gattgtattt cctgcatatg caccggataa aaataactga
     4141 tcaaatactc ttcttgtcgc aaatggtgca aatgtatgcg taaatggttt aaaccctgtc
     4201 aaagataatc cagctgcaac tcccatcata ttagcttctg caattccaca ctgaataaaa
     4261 cgctctggat ttgtcttttt aattttggta aatccacttg ctccacccaa atcagcttca
     4321 agagctgtta ttttgtcatc atccttcata agttccggca atgtctcaac aacgcaatct
     4381 cttaattctc ttcctgcttc ctgtctgttt tctgctaatt gaaacatttt acctggcacc
     4441 tcccttaatg aactcatcta actctttgat tgcatcctta gtagcttttt taatcgtatc
     4501 attattaaat tttacggagt gatttgcatc caactgttca aaatatggga ctccagctcc
     4561 ttttatggaa tcaagaataa tacatactgc ctggtctttt acatttttgg cccggttgat
     4621 tgcttcatca attgcttcct cgtcgtttcc ttttacagtc tgtgtataaa acccaaatgc
     4681 cttcatttta ttttcgatac taaatggatt cattacttct tttgttgtac catctaactg
     4741 ttttttgtta tcatcaataa tcacaacaca atgatttaat ttataatgcg caatatattg
     4801 aaatgcttcc cagcattgac cttcatttaa ttctccatct ccaacgatca ggtatacata
     4861 ctgatctgtt ttattcattt taaatcccg
//
LOCUS       DAOMUE010000139         4869 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4853_length_4869_cov_2.688201, whole genome shotgun sequence.
ACCESSION   DAOMUE010000139 DAOMUE010000000
VERSION     DAOMUE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4853_length_4869_cov_2.688201"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1118
                     /locus_tag="PLC71_06960"
     CDS             69..1118
                     /locus_tag="PLC71_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRM02417.1"
                     /translation="MTAQHTKHAKHTKRKRRRRSPLRILIILILVIAVLVGLTLSTVH
                     KKLEKISTKSLDQSQLVTVNKDSNMTDYTNYAFFGIDSQTGSMSDRGNRSDSIIVVSI
                     NNSTKDVKLLSIYRDTYVSINGKYSKINAAYSWGGPELAISTINRNLDLNIEKYATVN
                     FKILADIVDAVGGIEVKVDKSILKNLNAYIGDMNRINGGHSPKLSSAGTYTLDGNQAV
                     AYSRIRYTAGGDLARAGRQREVLQKIFDKAKKNPLKMMSVMDEILPQVKTNMSQDELF
                     DMFLSVFKYDIKDQQGFPWDQKELRYYGTYYGFPTTLKENAIRAHKYLFGTSDYQVSD
                     ELSRINQKIIYRAGY"
     gene            complement(1193..2362)
                     /locus_tag="PLC71_06965"
     CDS             complement(1193..2362)
                     /locus_tag="PLC71_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HRM02418.1"
                     /translation="MKKALGLAGKYVIMFLSCLFPRSRKIYIFGAWLGEQFADNPKYL
                     FLEAQEHKEIRPIWITKNESVCRKVRELGYEAYMFDSFKGILMQLRAKYVVVCNGISD
                     VNHTFMGRAVFLNLWHGVPLKKVGYDDDKVKNWDSKGQKIRRMIQEIPLGKEYVVATS
                     DFYAPIYESAFRRSKSHIITLGQPRNDIFYDQSGKFHASHQLSKAAKGKKVILYTPTH
                     RKEGKVAFPLEEHFDFKVLNDWCIQNDILFVIRRHFYHKDEKVDFSMYSNITDITERS
                     MDIQELLMDTDILVTDYSSTYIDYLLLDRPVVFYNFDYQDYLEHDRGMYCDYEKAAPG
                     YKAETFEALMEEFERLVQGEDHFKEVRRQARDFFYCKEGQGMVGETLLGILKNIK"
     gene            complement(2371..3306)
                     /locus_tag="PLC71_06970"
     CDS             complement(2371..3306)
                     /locus_tag="PLC71_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM02419.1"
                     /translation="MVTILLATLNGEDYLKAQLESIAAQTYENWQLVVGDDGSTDGTI
                     SILEEFSKQYPEQVTIIKNDPPQGSAKDNFISLIQNSYGPYFMFCDQDDVWKPDKIYL
                     TLQKMEALEARYGEKTPILVHSDLSVADHNLEMVAESFFQYANIPKRIVLNQLMVQNS
                     VTGCTMMINRCLQQYFLRPLPMSKIIMHDYWAALIAKVFGKIGFVNEPTMYYRQHGNN
                     SVGAKDAKNPVFLYHRLKEGKNSYRRMMIESMEQMQGFVETYGEEIDNLDVYELLESY
                     GELYYKGKIKRLSFYKNHHVLKEGNIRKLMQWLWG"
     gene            complement(3350..4522)
                     /locus_tag="PLC71_06975"
     CDS             complement(3350..4522)
                     /locus_tag="PLC71_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HRM02420.1"
                     /translation="MKQLIKNITIQIYKVLTYLIPVNKKMIIFQSSNGRNYTGNPRYI
                     YEEMVREGLDQEYECIWFFFDTSIEVPGKCKKIRNNYFPYFWYLMRAGIWVFDSRQPL
                     YVRKKKHITYIQTWHGTPLKKLALDMDRMDMGGSTDIENYHEQFRVTCKDWDYLVSQN
                     KFSTDIFRSCFAFKDRPILQIGYPRNDILINDNSPAKIREYKEKLGLPLDKKIILYAP
                     TWRDNEYSEKGKYKFASKLDFDKAKEALSDEYIFIVKYHYLVSDKIDWTPYKGFVYTF
                     DETKDIAWLYLVSDMLITDYSSVMFDYSILNRPMLFFAYDLEDYKENLRGFYFDFVND
                     IPGPISRDTDQLIKDIKEYNEDSWKEKYKNYHDKFNGIDDGHASQKVIDVIKKVSR"
     gene            complement(4533..>4869)
                     /locus_tag="PLC71_06980"
     CDS             complement(4533..>4869)
                     /locus_tag="PLC71_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRM02421.1"
                     /translation="MMKRYQVPFVIWANYDIKEQHIEKTSMNYIQSILTQTAGVKMTG
                     YQRFLNEVRKEVPTITSQGYWGKNGKFYQINDKGSPYYGIIQKYRMIQYNMMFDKKNR
                     RDSFFEVSK"
BASE COUNT         1460 a         1056 c          727 g         1626 t
ORIGIN      
        1 aggatggagt ttttcatcct ttttcctgta taaacttaac tatttaagga aatctggagg
       61 gatacattat gacagcacag catacaaaac atgccaaaca cacaaagcga aaacgaagaa
      121 gaagatctcc acttcggatc ttgatcattc tcattcttgt gatcgctgta ttagtcggac
      181 ttacgctttc cacagttcac aaaaaacttg aaaaaatatc aacaaaaagt cttgatcaaa
      241 gccagcttgt cactgtaaac aaagacagca atatgactga ctataccaac tatgcttttt
      301 tcggaattga ttctcagact ggaagtatgt cagatcgtgg aaaccgttct gattctatca
      361 tagttgtcag catcaacaat tcaaccaaag atgtcaaatt gttatcgatc tatcgtgata
      421 catatgtaag tattaatgga aaatacagta agatcaatgc cgcttattcc tggggtggtc
      481 cagagcttgc gatcagcacg atcaatcgta atcttgatct gaatattgaa aaatacgcaa
      541 ctgtaaactt taagattctt gctgatatcg ttgatgctgt tggcggaatc gaagtaaaag
      601 tcgataaaag catcttaaag aatttaaatg cttatatcgg tgacatgaat cgtatcaacg
      661 gtggacattc tccaaaactt tcttctgccg gtacttatac tctggatgga aatcaagccg
      721 ttgcttattc cagaatccgt tataccgctg gcggtgacct tgccagagcc ggaagacaac
      781 gagaagtcct ccagaagatt tttgacaaag ccaagaaaaa tcctttgaag atgatgtctg
      841 ttatggatga gatacttcct caggtaaaga caaatatgag tcaggatgaa ttgtttgata
      901 tgttcttatc tgttttcaag tatgacatca aagatcagca gggattccct tgggaccaga
      961 aagagctccg ttattatggt acttattatg gattccctac aacattgaaa gagaatgcga
     1021 tccgcgcaca taaatacctc tttggtacat cggattatca agtcagtgat gagcttagcc
     1081 gtatcaatca gaagatcatt tatcgtgctg gatattaatc ctgaatatca actcaaaatg
     1141 caaaaaagaa gctctcgatc tatggattca ctcatagatc aaaagcttct ttttatttta
     1201 tattcttcaa gattcctaac aatgtttctc caaccattcc ctggccttct ttgcagtaaa
     1261 agaaatccct tgcctgcctt ctaacctctt taaaatgatc ttcgccttgt accagacgtt
     1321 cgaattcttc catcaaggct tcaaatgtct ctgccttata tcccggagcc gccttctcat
     1381 aatcgcaata cataccacga tcatgctcca gataatcctg ataatcaaaa ttataaaaga
     1441 ctaccggccg gtccagtaat agataatcta tatatgtgct tgaataatct gtaaccagaa
     1501 tatccgtatc cattaataat tcctgaatat ccatggatct ctctgtgata tctgttatat
     1561 tagaatacat ggagaaatct actttctcat ctttatgata aaaatggcgc cggatcacga
     1621 acaaaatatc attttggata caccaatcat tgagcacttt aaaatcaaaa tgttcttcca
     1681 aaggaaatgc cactttacct tctttcctgt gtgtcggtgt atataagatg actttctttc
     1741 cttttgccgc ttttgacaac tgatgagaag catgaaattt tccactttga tcatagaata
     1801 tatcattcct tggctgtccc aatgttatga tatgactctt tgatcttcga aatgcacttt
     1861 catagatcgg agcataaaaa tcacttgttg ccactacata ctcttttcct aatgggattt
     1921 cctgaatcat tcttcggatt ttctgaccct tgctatccca attcttgacc ttatcatcat
     1981 catagccaac tttctttaat ggaaccccat gccaaagatt taagaatact gctcgtccca
     2041 taaatgtatg atttacatca ctgatcccat tgcagactac cacatatttg gcccgaagct
     2101 gcattaaaat tcctttgaaa ctgtcaaaca tataagcttc atatccaagc tccctgactt
     2161 ttctgcagac tgattcattc ttcgtgatcc atatcggacg gatctcttta tgctcttgtg
     2221 cttctaaaaa cagatattta ggattgtctg caaactgttc tccaagccat gctccaaaga
     2281 tatatatctt tctgcttctt ggaaataaac acgacagaaa catgatcaca tattttcctg
     2341 caagtcctaa tgcttttttc ataaatattc ctatccccaa agccactgca tcagctttcg
     2401 gatatttcct tcctttaata cgtgatgatt tttataaaaa ctcaatcgtt tgatctttcc
     2461 tttataatac aattctccat aactctcaag aagttcataa acatccagat tatcaatctc
     2521 ttccccatat gtctcaacaa atccctgcat ctgctccata gattcgatca tcatacgacg
     2581 atagctgttc tttccttctt tgagacgatg atacaaaaag acgggattct ttgcatcttt
     2641 cgcaccaaca gaattattgc catgctgacg gtaatacatc gttggctcat ttacaaaacc
     2701 aatcttacca aacactttcg cgatcaatgc cgcccaataa tcatgcatga tgatcttaga
     2761 catcggtaac ggccgtaaaa aatattgttg cagacaacga ttgatcatca tcgtacaccc
     2821 tgtcacagaa ttttgtacca tcaattgatt caatacaatt ctctttggaa tattcgcata
     2881 ctgaaagaat gactctgcaa ccatctctaa gttatgatct gcaactgaca gatctgaatg
     2941 aaccaagatc ggtgttttct ctccatatct tgcctctaac gcttccatct tctgtaatgt
     3001 cagataaatc ttatctggtt tccacacatc atcctgatca caaaacataa aatacggtcc
     3061 gtaagaattt tgaatcagtg aaataaaatt gtctttggca cttccctgtg gcgggtcatt
     3121 cttaatgatc gtcacttgtt ccggatattg tttactgaat tcttcaagaa tgctgatcgt
     3181 accatccgta gaaccatcat cacccacgac cagctgccag ttttcatagg tctgagctgc
     3241 aattgattct aactgcgctt ttaaataatc ctctccattc aatgtcgcta acagaatcgt
     3301 taccatattg ttttcaaacc tcctgactgt gttattgttc ggcagatctc tatctgctta
     3361 ccttctttat cacgtcaatg accttctgtg aagcatgtcc atcatctatg ccattgaatt
     3421 tatcatgata attcttatat ttttctttcc aactatcttc attatattct ttaatatctt
     3481 tgatcaactg atctgtatct cttgaaatcg gtcctggaat atcattgaca aagtcaaaat
     3541 aaaatcctct cagattctcc ttgtagtctt ccagatcata tgcaaagaat aacatcggcc
     3601 tgttaagaat actataatca aacattacag aagaatagtc cgtgatcaac atatctgaca
     3661 caaggtataa ccatgcaata tcttttgtct cgtcgaatgt ataaacgaat cctttatatg
     3721 gtgtccaatc aatcttatct gatacaagat aatgatattt tacaatgaat atatattcat
     3781 ctgataatgc ctcctttgct ttatcaaaat ctagttttga agcaaatttg tactttcctt
     3841 tctcgctata ttcattatct ctccaagttg gagcatataa tatgatcttc ttatccaatg
     3901 gaagtccaag tttttctttg tattctctga tcttagcagg agaattatca ttgatcaaaa
     3961 tatcattcct tggatatccg atctgtaaga tcggacgatc tttaaatgca aaacatgatc
     4021 tgaaaatatc tgtagaaaac ttattctgtg atactagata atcccaatcc ttacatgtta
     4081 ctctaaactg ttcatgataa ttctcaatat ccgtacttcc acccatatcc attcggtcca
     4141 tatcaagcgc aagcttctta agcggtgttc cgtgccatgt ctgaatgtat gtgatatgct
     4201 tcttcttacg cacatataat ggctgacgac tatcaaatac ccagattcct gctctcatta
     4261 aataccaaaa atatgggaaa taattattcc gtatcttctt gcatttacct ggaacttcta
     4321 tacttgtatc aaagaaaaac cagatacatt catattcttg atcaaggcct tctcttacca
     4381 tctcttcata gatgtatctt ggatttcctg tatagttacg tccattgctg ctctgaaata
     4441 taatcatctt tttatttact ggaatcagat atgttaatac tttatatatc tgaatcgtaa
     4501 tattcttaat cagttgcttc atacgtgcac tcctatttac ttacttcaaa gaatgaatct
     4561 ctgcgattct tcttatcaaa catcatattg tactgaatca tacgatactt ctgtattata
     4621 ccataatatg gtgatccttt atcattaatc tgataaaatt ttccattctt tccccaatat
     4681 ccctgcgatg tgatcgttgg aacttctttt cggacctcat ttaaaaatct ctgatatcct
     4741 gtcatcttta ctccagctgt ctgggtcagg atactttgga tatagttcat gcttgtcttc
     4801 tctatatgct gttctttaat gtcataatta gcccagatca caaatggtac ctgataacgc
     4861 ttcatcatc
//
LOCUS       DAOMUE010000140         4865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4860_length_4865_cov_2.628274, whole genome shotgun sequence.
ACCESSION   DAOMUE010000140 DAOMUE010000000
VERSION     DAOMUE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4860_length_4865_cov_2.628274"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PLC71_06985"
     CDS             complement(<1..414)
                     /locus_tag="PLC71_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02422.1"
                     /translation="MYYSTATGYIIILSPLILLALLATIGIVLYRHPSKLQRKWYSVS
                     FKKRKRIKQIAILLGFCMAAGLLIYPLTSKKQFVSTISLSANENNFITEDKIDQISED
                     RREIKNEQWLKKFYKMMKSDDESSTLLNDIGSYEVW"
     gene            complement(414..>718)
                     /locus_tag="PLC71_06990"
     CDS             complement(414..>718)
                     /locus_tag="PLC71_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02423.1"
                     /translation="VTMASFDHFAQILLIQIVLIAVFFGVAFYVFQKSDMAKRNNFMY
                     FPWMEHLFVWIFPFCVSMFIVVTGFKIQSYTGGILAAVIATIIYYIVEKRKKRRAV"
     assembly_gap    719..728
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<729..1451)
                     /locus_tag="PLC71_06995"
     CDS             complement(<729..1451)
                     /locus_tag="PLC71_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02424.1"
                     /translation="MRGLVNHEKRNLLAITLVYTIVICFILQYVIANDISGTADQINN
                     FYGGIYNNYGNLFLGTLDQYYEMFIIVLMIPVSFVAVIQYRESSTSKCGEFLMQLPIK
                     RGQIFLTRTITGMMTYTIPWLFLSFGMIMMRTKAQSWYEMNLSVCKNGAVLLGNDNII
                     HLCAYLLFIWVSLTLIYAIAIFFQNICKRPWIAGAIGIGAMLFPNFMDYMMPKVLSFP
                     DTIYHGWWMAAIFENGPITKQFI"
     gene            complement(1435..2526)
                     /locus_tag="PLC71_07000"
     CDS             complement(1435..2526)
                     /locus_tag="PLC71_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02425.1"
                     /translation="MKFFRKYNLVLPPAALLYGIFVWNMKYPGDGLPQTMMDDLVNGW
                     LLIIILFAALSAGIVYQLQQNKKQQYIYSLPWTKKDIYQKSLTKLHISLLFAEIIYGI
                     FFAMKIATIPNAESITNVIICTLWNAVMCFAFCALIQVSLIVTAYIWQGLVIAVVTFY
                     VILPMILQNLAFLLQLLFKVRSSRFAILEWVIYGRFGRTFLYPLNFQLDTNSLESNPI
                     WKAQYVNSAIICIAVLFAAGVFSIWFAKRQFIKQEQAQNRMLTRLSGMQNKVITTIVI
                     TILCFNALSNHEIYVYIFKYDKTLRTRIYGLIAWGKEFMCSSDSYKLIIQKLNAVHWF
                     IYLVVILVITYFAVSIIQTIRRKCYERAC"
     gene            complement(2543..3415)
                     /locus_tag="PLC71_07005"
     CDS             complement(2543..3415)
                     /locus_tag="PLC71_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM02426.1"
                     /translation="MKVIELKDVKKSYGKNEILHGISLDIEEGSIHGLVGRNGCGKTT
                     LIKCLTGIYEQDQGQILVCGEEIFENPNVKAQVGYVADSNQYFEGYHIDEMVEFYKQI
                     YPTFDEKVFRDYNQSIGLDVSKRIKELSKGQAMSLASMLNLSIHPKVMIMDEPMSGLD
                     VIAQKQIKDFIVNEVDMNGMSVLISSHDLKDLESFCDSASMMKDGKILYQGTMDQMKE
                     RFTKLQVVFGESRSEIFKELPGVITYSNLGSVYTVILEGYGEPIHEALKQAGAIVVEE
                     IPLSLEEVFVYSNR"
     gene            complement(3419..3796)
                     /locus_tag="PLC71_07010"
     CDS             complement(3419..3796)
                     /locus_tag="PLC71_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRM02427.1"
                     /translation="MFRINPASGKAIYEQIMEQVKEAAWKGYLKPGDALPSVRKLALS
                     LNVTPNTVAKAYQLLEKEKVIITIRGKGAFLAENASQEVDKGKMQEIKTSLKEILSEL
                     RYEGYDEEKIASLIHEIYQDLEG"
     gene            complement(4036..4674)
                     /gene="dhaL"
                     /locus_tag="PLC71_07015"
     CDS             complement(4036..4674)
                     /gene="dhaL"
                     /locus_tag="PLC71_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HRM02428.1"
                     /translation="MSSFKNADAGVIVDQLIKTIQKERDYLSEIDGKIGDGDHGINMN
                     KGFTMCEKKLEGTTYNLSEGLTTLGETLLEDIGGSMGPLYGMLFDEMAQACEDEEEIT
                     ADVFAEMITASVEGIQDISPAQVGDKSLLDTLIPAKEAFVEAKEAGKDFAECLDAMNA
                     AAEKGWQSTKDLVAKIGRASRLGERSRGVLDAGATSCYFIITTIGSTAKSLL"
BASE COUNT         1365 a         1063 c          725 g         1702 t
ORIGIN      
        1 ccatacttca taacttccta tgtcatttaa aagagtcgaa ctttcatcgt ctgatttcat
       61 catcttataa aattttttca gccattgttc attttttatt tctcttctgt cttctgaaat
      121 ctgatcgatc ttatcttctg tgataaaatt gttttcattt gctgacagac tgatcgttga
      181 tacgaactgc tttttgcttg tcagcggata gataagtagt cctgctgcca tacaaaatcc
      241 aagaaggatc gctatttgtt taattctttt tcttttctta aatgatacgc tataccattt
      301 tctctgtaat tttgacggat gacggtaaag tacgattccg atcgttgcca ataaagctag
      361 aaggatcagt ggtgataata tgataatata accagttgca gttgaataat acattatact
      421 gccctccttt tcttacgctt ttctactata taatatatga tcgttgcgat cactgctgcc
      481 agaatgccgc ctgtgtatga ctgaatcttg aaccctgtca ctacaatgaa cattgaaaca
      541 cagaatggga agatccatac aaacagatgc tccatccatg gaaaatacat aaaattattc
      601 ctctttgcca tatccgactt ttgaaataca taaaatgcaa ctccaaaaaa taccgcgatc
      661 aatacgatct gaatcagaag gatctgtgca aaatgatcaa acgaagccat cgtcaccann
      721 nnnnnnnnta taaactgttt tgtgatcggt ccattttcaa aaattgcagc catccaccag
      781 ccatgatata ttgtatctgg gaatgataat acttttggca tcatataatc cataaagttt
      841 ggaaataaca ttgctccgat tccgatcgct cctgcgatcc atggtctttt gcagatattc
      901 tgaaaaaata tagcaatggc atagatgagt gtcaatgata cccatataaa tagaagatac
      961 gcacacaaat gtatgatgtt atcatttcca agaagtactg ctccgttttt acatacggat
     1021 agattcatct cataccatga ctgtgccttt gttctcatca tgatcattcc gaatgaaagg
     1081 aaaagccatg gaatcgtata tgtcatcatt cctgtgatcg tccttgtcag aaaaatctga
     1141 cctcttttga tcggaagctg cattaaaaat tctccacatt ttgatgtact gctttcccga
     1201 tactgtatta ccgctacaaa actaaccggt atcataagaa cgataatgaa catttcatag
     1261 tattgatcta atgttcctaa aaacagattg ccataattat tataaatgcc tccataaaaa
     1321 ttattgatct gatctgcagt tcctgaaata tcgtttgcga tcacatattg taatatgaaa
     1381 catatcacta tggtatacac taatgttatg gctagcagat ttcgtttttc atgattaaca
     1441 agccctctca taacatttcc tccttattgt ctgaataatc gatacagcaa aatatgtgat
     1501 cactaatatc actaccaaat atataaacca atgtactgca tttaatttct gtatgatcag
     1561 tttataacta tctgatgaac acataaattc ttttccccat gcgatcaatc cataaattct
     1621 ggttcgaagt gtcttgtcat acttaaatat ataaacatat atctcatgat tacttaatgc
     1681 attaaaacac aggatcgtga tcactatcgt cgtgatgact ttattctgca tccctgataa
     1741 ccttgttaac attcggttct gtgcctgctc ctgcttgata aactgtcgtt ttgcaaacca
     1801 gatactaaag actcctgctg caaataaaac ggcaatacag atgatcgcag aatttacata
     1861 ttgtgctttc cagatcggat tgctctccaa gctatttgta tcaagctgaa aatttaatgg
     1921 atataaaaat gttctgccga atcttccata gataacccac tcgagtatcg caaatctaga
     1981 agatcttacc ttaaacagca actggagtaa aaatgccaga ttctgaagga tcatcggcaa
     2041 tatcacataa aatgtaacga ctgctatgac aagtccttgc cagatatatg ctgtcacgat
     2101 caatgaaacc tgtatcagtg cacaaaatgc aaaacacatt actgcattcc ataatgtgca
     2161 tatgatcaca ttcgtgattg attccgcatt cggaatcgtt gcgatcttca ttgcaaagaa
     2221 aattccatag atgatctctg cgaacaaaag tgatatatgc agttttgtaa gactcttttg
     2281 gtaaatgtct ttctttgtcc atggaaggct gtagatatat tgctgttttt tattttgctg
     2341 cagttgatat acaattcctg ctgataatgc tgcaaataaa attatgatca aaagccatcc
     2401 attgacaaga tcatccatca tcgtctgtgg cagtccatcc cctggatatt tcatgttcca
     2461 tacgaaaatc ccatataaca atgctgctgg tggaagtacc agattatatt ttctaaaaaa
     2521 tttcatctcc aagttcctcc tcttagcggt tgctgtagac gaatacttct tctaaactta
     2581 atggaatttc ctctactaca atcgctcctg cttgttttaa tgcctcatga attggctctc
     2641 catacccttc taatattact gtgtatacac ttccaagatt agaatacgtg atcactcccg
     2701 gaagttcttt gaagatctcg cttcttgatt ctccaaatac aacctgtaat tttgtaaatc
     2761 gttctttcat ctgatccatt gttccctgat aaaggatctt tccgtccttc atcatacttg
     2821 cggaatcgca gaaactttct aaatccttta aatcgtgtga tgagatcagc acactcattc
     2881 cattcatatc gacttcattg acaataaaat ctttgatctg tttctgtgcg atcacatcaa
     2941 gtcctgacat tggttcatcc atgatcatca cttttggatg aatggacaga ttcaacatcg
     3001 aagctaatga cattgcctgt cctttggata attctttaat ccttttgctt acatctaatc
     3061 cgatactctg attgtagtcc ctaaatactt tctcatcgaa tgttgggtag atctgtttat
     3121 aaaattctac catctcatcg atatgatacc cttcaaaata ctggttgctg tctgcaacat
     3181 atcctacctg tgcttttaca tttggattct cgaaaatttc ttctccacag accaagatct
     3241 gtccttgatc ctgttcataa atcccagtta agcatttgat caatgttgtt tttccacatc
     3301 cattgcgtcc taccagccca tgaatgcttc cctcttctat atccaggctg attccatgaa
     3361 gaatctcatt ctttccatat gattttttca cgtcctttag ttcgatcact ttcatatcct
     3421 atccctccag atcctgatag atctcatgta tcaggcttgc tatcttctct tcatcatacc
     3481 cttcataacg aagctcactt aatatctctt ttaaacttgt cttgatctcc tgcatctttc
     3541 ctttgtccac ctcttgtgaa gcattttctg ccaaaaatgc tccctttcct cgtattgtta
     3601 taattacttt ctctttctcc aacaactgat atgctttcgc tacagtgttg ggtgttacat
     3661 tgagacttaa tgctaattta cgaacggacg gcaatgcatc tccaggtttt agatatcctt
     3721 tccacgctgc ttcttttact tgttccatga tctgttcata aattgccttg ccactggcag
     3781 ggttgattcg aaacacacct gtacctcctt attgtttttg tattactgta tgccatactg
     3841 tattagctgt actaactgta ccatttcata tgatacagtt agtttatata ctttttgcct
     3901 gtttgtcaat aactttttta aaatttccta tgaaatgaaa gaaccccaaa gcttctttcc
     3961 gaaactttga ggttcttcct aaacaaattg tatgaatata tagagggttc attgctttcg
     4021 caatttaaat cattcttata acaggctctt tgctgtgctt ccaatagttg tgatgataaa
     4081 ataacagctg gttgctcctg catcgagaac acctcgagag cgttctccaa gacggcttgc
     4141 tcgtccaatc ttcgctacaa gatcttttgt agactgccat cctttttctg ctgctgcatt
     4201 catagcatcc aaacattctg caaagtcttt tcctgcttct tttgcttcta caaatgcttc
     4261 ttttgctgga atcaatgtgt ctagcagtga tttgtctcct acctgtgcag ggctgatatc
     4321 ctgaattcct tctacagagg ctgtgatcat ttctgcgaat acatctgctg tgatctcttc
     4381 ttcatcttca catgcctgag ccatctcatc aaataacatt ccatacagag gtcccattga
     4441 tccaccgata tcttctaaca gtgtctcacc taatgttgtc agtccttctg acaaattata
     4501 tgtagttcct tctaatttct tctcgcacat cgtaaaacct ttgttcatgt tgatcccatg
     4561 atctccgtct ccgatcttac catcaatctc acttaagtaa tctctttctt tctgaattgt
     4621 cttgatcagc tggtctacga taactcctgc atcggcattt ttaaaacttg acataatttc
     4681 cacctctttg aattaatgtt gtacttttct tatctatgtt tattttaaca tacaatcttt
     4741 tttacctgaa atgattttca gttcaaatat ggcactcatt atggtgctat gtttcactct
     4801 tggttgttgt aggtttatat aacgtactct aaaatcgctt ccggtttctc tacaatatat
     4861 gtagc
//
LOCUS       DAOMUE010000141         4853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4874_length_4853_cov_1.921842, whole genome shotgun sequence.
ACCESSION   DAOMUE010000141 DAOMUE010000000
VERSION     DAOMUE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4874_length_4853_cov_1.921842"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..664)
                     /locus_tag="PLC71_07020"
     CDS             complement(125..664)
                     /locus_tag="PLC71_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HRM02429.1"
                     /translation="MIEREKLQHLIENIGIPKLCLILLLGISLIIVSIPSGKTKKNAQ
                     STETTAKEDSTDLYVKKQERRLVNALKHVEGVGKVKVMITVNSSKEAVVNKDSPYEQS
                     SSEKEKTVKEDEQTILVEEDGKKVPYVIKEVEPVVEGVVVVAQGGGNDIIKNQIVEAV
                     SVLFHISSYKVKVLKMEDS"
     gene            complement(672..1115)
                     /locus_tag="PLC71_07025"
     CDS             complement(672..1115)
                     /locus_tag="PLC71_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HRM02430.1"
                     /translation="MDYLKMIIFFLILTTLIDQLCDKSIYRPYLRLMTGFMLISLMMQ
                     PISYYLSNEDIVTQAISTAVKGENNLAFLDGAKEEKQNQIKEQITTLFSQYDLKVLDV
                     DSKVNEKGTIEKIDIKTDKLRHTKKLKTAISNFYNMKESNINISE"
     gene            complement(1121..2248)
                     /locus_tag="PLC71_07030"
     CDS             complement(1121..2248)
                     /locus_tag="PLC71_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HRM02431.1"
                     /translation="MKQRIFLLTLLFLLILTRTNDISAKETKLNEDMFTNVENTLKDK
                     EIDLSYTQLVKQLIHGNVKGVLHTIKAKMKEGFLNRMLIQKDLIREILLIAFTAAIFK
                     NLSDSFFQQSTANSAFYITYVILCGVMVHSFFYLNKTVTQLVTLMADYMKGMIMAYSL
                     AVVSTSGITTSTTVYEFYLLLIYAMTTLTNVVLLPMIKILFVLKIINHISKEEHFSRL
                     CKTLEGVIKFLLKGFLSVLLGVQLIQSMILPAVDSMKNTVLQKGMSAIPGIGSGLNSA
                     MTMVIGSAVVIKNSIGAVGILVLIVIIVPPVIEITAVVLTYLLAGIMIQPISDKRITG
                     AMDAVIQSGKLMLSMIFTMALLFILSVALIAFSTNVNYYAG"
     gene            complement(2245..2634)
                     /locus_tag="PLC71_07035"
     CDS             complement(2245..2634)
                     /locus_tag="PLC71_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HRM02432.1"
                     /translation="MIKVAVFGIVASLIAMKIKTIRPEIAVVIAVISSILLAMYGLKQ
                     MEEILTVFEMIRSYSKIPQSYFQILLKLIGISFICEFASNICKDAGQASIAKQIEFAG
                     KLAILIVGLPVFESLLGTIQKLLGNGT"
     gene            complement(2656..2853)
                     /gene="spoIIIAC"
                     /locus_tag="PLC71_07040"
     CDS             complement(2656..2853)
                     /gene="spoIIIAC"
                     /locus_tag="PLC71_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HRM02433.1"
                     /translation="MSVNIIFRIAAVGILVTILVQVLKHSGRDEQAFLITLAGLILVL
                     GWVIPYIYDLFESVQTLFTIS"
     gene            complement(2890..3408)
                     /locus_tag="PLC71_07045"
     CDS             complement(2890..3408)
                     /locus_tag="PLC71_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HRM02434.1"
                     /translation="MIKILGSLIVIFAGIGFGFHKAAKEQKRLEQGIAIKRMLYLLQG
                     EIRYGFTPLPEAVLKISTKTEKEYQPFLKKVAKQLETHSEESFAKVWQQTAEQKLKQV
                     IYEPKFYEILKNMGETIGYLDQQMQEKTILLTIEQLDDQIFLMKDQVVKNCKMYRSLG
                     ISLSVLIVIILL"
     gene            complement(3405..4331)
                     /gene="spoIIIAA"
                     /locus_tag="PLC71_07050"
     CDS             complement(3405..4331)
                     /gene="spoIIIAA"
                     /locus_tag="PLC71_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HRM02435.1"
                     /translation="MKEEEILGILPLHIRTIIKQAGINWDELQEIRLRIQKPVILKYN
                     AKTCYLSEQGKITTRKEQLHIVTSQEIRQAMEYISSYSMYAYIEQLKQGFLTNRGGHR
                     VGICGTVVMDHDKVKTIRHISGLNIRVAHEVKGCADTLYSRCTRMGKLIPTLIISPPG
                     CGKTTLLRDMIRKISDEGQTVGVVDERSEIGACYQGVAQNDLGIQTDVMDACEKGQGM
                     NMLIRSMAPDVIAVDEIGSKEDVEALFFCAYRGCSILATTHGKNKESLSKNPYMREII
                     NKKMFQRYVILEQRNQPGYIAQIIDENGVVLN"
     gene            complement(4405..>4853)
                     /locus_tag="PLC71_07055"
     CDS             complement(4405..>4853)
                     /locus_tag="PLC71_07055"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRM02436.1"
                     /translation="APKRYNSGSLILAMENAGQLIEDEDLREQIKGSGIGTSATRAEI
                     VKKLVSNKYIALNKKTQIVTPTLTGEMIVNVVSASIGSLLNPTLTASWEKGLTYVAEG
                     SVTEEEYMQKLDKFVTQKTNIVKQNNFQYQLRQSFDQIAPYYQKKK"
BASE COUNT         1212 a         1009 c          959 g         1673 t
ORIGIN      
        1 gtcttactat tcttcgtccc agccggatcg tatttctgac tataatttac ataccctgca
       61 acagcgatca caaccgctaa ggtcatgatg atcatctgat ttttctgaaa tacttttttc
      121 atgtttagga atcctccatt tttaagactt tcactttata agatgaaata tgaaataaca
      181 cagaaactgc ttcaacgatc tgattcttta taatatcatt cccgccgcct tgggcgacaa
      241 cgaccactcc ttcaacgact ggttctactt ctttgatcac gtatggaact ttctttccgt
      301 cttcttcgac taagatggtc tgttcatctt ctttgaccgt tttttctttt tcactgctgc
      361 tttgttcgta gggactatct ttatttacga cagcttcttt ggatgaatta acggtgatca
      421 tcacttttac ttttccgact ccttctacat gttttaatgc attgaccaat cgcctctcct
      481 gctttttcac atagagatcg gtagaatctt cttttgctgt ggtttcagtg ctttgtgcgt
      541 tcttctttgt cttcccggat gggatgctta cgatgatcag gctgatgcca agaagtaaga
      601 tcaggcaaag ttttggaatg ccgatatttt cgatcagatg ttgtagtttt tccctctcta
      661 tcatatttct tctactcact tatatttata ttagattcct tcatattata gaagtttgaa
      721 attgcggttt ttagtttctt tgtatgacga agcttgtctg ttttgatgtc gatcttctcg
      781 atggttcctt tttcatttac ctttgagtca acgtctaata ctttgagatc gtattgtgaa
      841 aataacgttg tgatctgttc tttgatctga ttttgttttt cctcttttgc accatctaaa
      901 aatgcaaggt tattttctcc tttgactgct gttgagattg cctgtgtcac gatatcttcg
      961 ttggataaat aataggatat tggctgcatc atcagggaga tgagcataaa tcctgtcatt
     1021 aaccgaaggt atggacggta gatgcttttg tcgcagagct gatcgatcag tgttgttaag
     1081 atcagaaaaa atatgatcat tttcagataa tccataatcc ttaccccgca taataattaa
     1141 cgttggttga gaatgcgatc aatgccacgg acaggataaa taatagtgcc atcgtaaaga
     1201 tcatcgaaag cattaatttc ccgctttgaa tgactgcatc catcgctccg gtgatccttt
     1261 tgtcggatat gggctggatc atgatccctg ccagcagata ggtaagaaca actgctgtga
     1321 tctctataac tggtggcacg atgatcacga tcagtacaag gattccaacg gctccgatgc
     1381 tgtttttgat cacaactgcc gaaccgatga ccatggtcat ggctgaattc aaaccacttc
     1441 cgattcctgg aatggcactc attccttttt ggagaactgt atttttcatg gaatcaacgg
     1501 ctggaaggat catcgactgg atcagctgaa ctcctaacag gacacttagg aatcctttga
     1561 gcaaaaactt aatcacacct tcgagcgttt tacatagtct tgaaaaatgt tcttcttttg
     1621 atatatggtt gatgatcttt aagacaaata agatcttgat catcggaagt aatacaacat
     1681 ttgtaagggt tgtcattgca taaatcagca gaagataaaa ttcataaact gtcgttgatg
     1741 tcgtgatccc ggaagttgag acaacagcca gtgaatatgc catgatcatt cctttcatat
     1801 aatctgccat taaggtaacg agctgcgtca ctgttttgtt taaataaaaa aaggaatgta
     1861 ccatgacacc gcataggatc acataagtaa tataaaatgc actattggct gtgctttgct
     1921 gaaaaaagga atcggacaga tttttaaaga tggctgcggt aaatgcgatc agaaggattt
     1981 ctctgatcag gtctttttgg atcaacatac ggtttaaaaa gccctctttc attttggctt
     2041 tgatcgtatg cagaacacct tttacatttc cgtggatcag ctgttttact aattgtgtat
     2101 aagatagatc gatctctttg tcttttaagg tgttttccac attggtaaac atatcttcgt
     2161 tgagttttgt ttctttggct gaaatatcat ttgttcttgt aaggatcagt aaaaacagca
     2221 aggttagcag gaatattctt tgtttcatgt gccatttcct aatagtttct ggatcgttcc
     2281 aagcagcgat tcaaagactg gaagtccaac gatcaggatc gctaattttc ctgcaaactc
     2341 gatctgcttt gcaatcgatg cctgtcctgc gtctttgcag atattggatg cgaactcgca
     2401 gatgaaggag ataccgatca gttttaaaag gatctggaag tagctttgtg ggatttttga
     2461 ataggaccgg atcatctcaa acacggttag tatttcctcc atttgtttta gtccatacat
     2521 tgccagaaga atgcttgaga tcactgcgat cacgactgcg atctctggac ggatcgtttt
     2581 gatcttcatg gcgatcagac ttgcaacgat cccaaagact gcgactttta tcatcttagt
     2641 atccgcctcc aatctctagg atatcgtaaa aagtgtctgg acgctttcaa acagatcata
     2701 gatatacggg atcacccagc caaggacaag gatcaatccg gcaagtgtga tcaaaaatgc
     2761 ctgttcatct cttccggaat gttttaagac ctgcacaagg atcgttacaa gaattccaac
     2821 ggctgcgatc cgaaaaatga tattgacact cataacccct ccttgtcata aaaatgtctg
     2881 gtagttgctt tatagtaaga tgatcacgat cagaacgctt agtgatatac cgaggcttcg
     2941 gtacatcttg cagttcttta ctacttgatc tttcataagg aagatctggt catcgagttg
     3001 ttcgattgtc agcaggatcg ttttctcctg catctgttgg tcaagatatc cgatcgtttc
     3061 acccatgttt tttaagattt cataaaactt tggttcatag atcacttgtt ttaatttctg
     3121 ctcagctgtc tgctgccata ctttggcaaa agattcttcg ctgtgtgtct ctaactgctt
     3181 tgccaccttt tttagaaatg gctgatattc cttctctgtc tttgtcgata tttttaaaac
     3241 agcttctgga agtggggtaa atccatagcg gatctctcct tgcagaaggt ataacattcg
     3301 tttgatcgca attccctgtt ccagacgttt ttgttctttt gcggctttgt gaaatccaaa
     3361 tccgatccct gcaaagatca cgatcaatga tcctaatatc ttgatcaatt taggaccacc
     3421 ccgttttcat ctatgatctg tgcgatatag ccaggctggt ttcgctgttc taagatcacg
     3481 tagcgctgaa acatcttttt gttgatgatc tcccgcatat agggattctt ggataacgat
     3541 tctttgttct ttccatgggt cgttgccaga atgctgcagc cacggtaggc acagaaaaat
     3601 aaggcttcca catcttcttt gcttccgatc tcgtccacag cgatcacatc gggggccatg
     3661 gatcggatca gcatattcat gccctgtcct ttttcacatg catccatgac atctgtctga
     3721 atcccgagat cattttgggc aacgccttgg tagcatgctc cgatctcact tcgttcatcg
     3781 acaactccga cggtctgtcc ttcatcagag atttttcgga tcatatcccg aagcagagtt
     3841 gtttttccac agccgggtgg tgagatgatc agcgttggaa tgagttttcc cattctggtg
     3901 cagcgtgaat ataaagtgtc cgcacatcct ttcacttcat gtgcgactcg gatatttaac
     3961 ccggaaatat gtcggattgt tttgactttg tcatgatcca tgacaacggt cccacagata
     4021 ccaactcggt gtccgcctcg gtttgttaaa aatccttgtt ttaactgttc gatataagca
     4081 tacatcgaat agctgctgat atattccatg gcttgtctga tctcttgtga ggttacgata
     4141 tgtaattgtt cttttcttgt tgtgatcttt ccctgttcgg atagataaca ggtttttgcg
     4201 ttgtatttta aaatgactgg cttttgaata cgcaggcgga tttcctgaag ttcgtcccag
     4261 ttgatgcctg cctgctttat gatcgttctg atatgtaatg gaaggattcc taagatttct
     4321 tcctctttca taaatttaaa aacacctccc cattttggta taatccaaat atatgtgatg
     4381 gaaggtgttt ttatgaatgt tattttattt tttcttctga taatatggtg cgatctggtc
     4441 aaaggattgt ctgagctgat actggaagtt attttgcttg acgatatttg tcttctgggt
     4501 gacaaacttg tctaacttct gcatatactc ttcttccgtg acacttcctt ctgccacgta
     4561 agtaagacct ttttcccagc ttgcggttaa ggttgggttt aaaagtgatc ctatggatgc
     4621 actgacaaca ttgacgatca tctctccggt taaagtcggt gttacgatct gtgtcttttt
     4681 atttaatgca atgtatttat tggacactaa tttctttacg atctctgctc ttgtcgcact
     4741 cgttccgatc ccgcttcctt tgatctgttc tcttaaatct tcatcttcga tcagctgtcc
     4801 tgcattttcc atcgcaagga tcagggaacc tgagttgtat cgttttggtg cgg
//
LOCUS       DAOMUE010000142         4848 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4883_length_4848_cov_3.384936, whole genome shotgun sequence.
ACCESSION   DAOMUE010000142 DAOMUE010000000
VERSION     DAOMUE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4883_length_4848_cov_3.384936"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLC71_07060"
     CDS             complement(<1..463)
                     /locus_tag="PLC71_07060"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRM02437.1"
                     /translation="MLVSAKEMLNKAKAGHYAVGQFNINNLEWTKSILLAAEETRSPV
                     ILGVSEGAGKYMTGYKTVVGMVNGMLEELNISVPVALHLDHGSYEGCYKCIEAGFSSI
                     MFDGSHYPIEENIEKTKELVKVAKGKGMSVEAEVGSIGGEEDGVIGKGECAD"
     gene            complement(586..1341)
                     /locus_tag="PLC71_07065"
     CDS             complement(586..1341)
                     /locus_tag="PLC71_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HRM02438.1"
                     /translation="MKEWKEYLFKVNGFEMTAVYNEDTIQKVFLPLLKQLQQLQKEKK
                     ERIIVFMAAPPAVGKTTLCEFLEYLSKQDQEFTDIQALGLDGFHYHSDYINSHDAIVL
                     GEKVPMKQVKGCPETYDTEKLRQKLEKIKIEDILWPIYDRNLHDVVEDQIKVTKDIIL
                     IEGNWLLLKQEPWKSMQQYADYKILILAEEEMLKERLISRKEKGGLTREEAVAWYQNS
                     DSKNVTRVLKNSCRKHLNLLLQVEQDNDYLQVK"
     gene            complement(1482..1850)
                     /locus_tag="PLC71_07070"
     CDS             complement(1482..1850)
                     /locus_tag="PLC71_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HRM02439.1"
                     /translation="MKMMTLYKIIRSMPCFFKEVTHFYCPACGGTRSVIALLHLDIER
                     AFLCNPTVVYTGVMFLWCIAGWMVKKLTAREMKSMKPRLWMLILGVCIFFGYAVIRNI
                     LVYQFGYDYLGDLIHYTEFN"
     gene            complement(1828..2292)
                     /locus_tag="PLC71_07075"
     CDS             complement(1828..2292)
                     /locus_tag="PLC71_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HRM02440.1"
                     /translation="MEDKRYDPYTGYEIKDEDPTQEYHTQDDDPYRRYRYEDDHIEQP
                     QQEQTSGIAIATMILGIIAVVCMVSCCCTPIAIITGIAAVICFAVTPKVRGTRGNKAK
                     AGLICGIIGIIGSVILITVFVVNILLSSDFQNEMKEFNTHYDTYIDENDDSI"
     gene            complement(2303..2728)
                     /locus_tag="PLC71_07080"
     CDS             complement(2303..2728)
                     /locus_tag="PLC71_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRM02441.1"
                     /translation="MKLVTLVSNNVGVDTYLELRKAVNFKPLSRTQARKALDNSLYIV
                     CAYIDAKIVGMGRLVGDGAVICYIQDLMIHPDYQHYGIGSAIIEDLIEYVENLCEEGT
                     EIMLDLMCAIGREPFYHKHEFISRPTDKLGPGMIRYIRK"
     gene            complement(2948..3298)
                     /locus_tag="PLC71_07085"
     CDS             complement(2948..3298)
                     /locus_tag="PLC71_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRM02442.1"
                     /translation="MLIKINAIVREEKFEDVKEALNKIGVNGITVSQVMGCGAQKGFT
                     EVIRGTKVDMMMRPKIKFEIVVSSEEWERKTIEAIQKSAFTGEVGDGKIFSYEVRNVV
                     KIRTGERDVDALQD"
     gene            complement(3314..>3998)
                     /locus_tag="PLC71_07090"
     CDS             complement(3314..>3998)
                     /locus_tag="PLC71_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia permease"
                     /protein_id="HRM02443.1"
                     /translation="GYDQGVYHVHNTPFVFLGAALLWFGWYGFNAGSALAANGLAAHA
                     FMTTSVSAAAALVSWMLIEVFSEGKTTLVGASTGLVIGLVAITPGAGFVPMWAAVICG
                     LLVSPICYFGVKLIKGKLKIDDALDAFGCHGIGGIWGGIATGLFGMTSINGVAKWNGL
                     VFGETRLFVAQIIGILVSIAVAVVGSLICIAIVRIFTPLRVEERAEKVGLDVSEHGEN
                     AYPSFNGLD"
     assembly_gap    3999..4008
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4009..4547)
                     /locus_tag="PLC71_07095"
     CDS             complement(<4009..4547)
                     /locus_tag="PLC71_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia permease"
                     /protein_id="HRM02444.1"
                     /translation="MNAGDTAFILLCAAFVFIMTPGLAFFYGGMVRRKNVGNTMMQCV
                     FIMGVSVIMWVLVGYALSFGGNHAGIIGGAKWFGFNGVGMKPGPYADTIPNLAFAAFQ
                     MMFAMITPALITGSVAGRMKFKALVLFIILWSLIVYYPMAHMVWGEGGFLAEIGSVDF
                     AGGNVVHITSGVSGLVLALT"
     assembly_gap    4680..4689
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1349 a         1096 c          706 g         1677 t
ORIGIN      
        1 gatctgcaca ttcaccttta ccgataactc cgtcttcttc tccaccgata gatccaactt
       61 cagcttcgac agacattcct tttccttttg caacttttac aagttctttt gttttttcaa
      121 tgttttcttc gattggatag tgagatccat caaacatgat agaagagaat ccagcttcga
      181 tacatttgta gcatccttca taagaaccgt ggtctaagtg aagagctact ggtacagaaa
      241 tgtttaattc ttctaacatt ccgttaacca ttcctacaac agttttgtat cctgtcatat
      301 attttcctgc tccttcagaa acaccaagga tcactggaga tcttgtctct tctgctgcta
      361 ataaaataga ttttgtccat tctaagttat tgatgttgaa ctgtccaact gcgtaatgtc
      421 cggcttttgc cttgtttaac atttcttttg ctgatactaa catagtttgt cctccatata
      481 attcgttaac tttttcacat ctagtttatt atactctatt tatacgtggg ttgcaagctt
      541 tcacttgtta ctaatatctt ctgttttcct gcaaaaattt ggtcattatt ttacttgcaa
      601 ataatcgtta tcctgttcca cttgtaacag cagattcaaa tgtttccggc aggaattttt
      661 taaaactctt gtcacatttt tactgtcact gttttgatac catgccacag cttcttctct
      721 tgtgagacct cctttttcct ttcttgagat cagacgttct ttcaacatct cttcctcggc
      781 caggatcaag attttatagt ctgcgtattg ctgcatggat ttccatggtt cttgttttaa
      841 tagtaaccag ttcccttcga tcaaaatgat atctttggta actttaatct gatcttccac
      901 aacgtcgtga agatttctat cataaatcgg ccataaaata tcttctattt ttatcttctc
      961 aagtttctgt cttaattttt ctgtgtcata tgtttccgga catcctttta cctgcttcat
     1021 tggaactttt tctccaagca cgatcgcatc atgagaatta atgtaatcag agtgataatg
     1081 aaatccatct aatcctaatg cttgaatgtc tgtaaactct tgatcttgtt ttgataaata
     1141 ttccaaaaac tcacataatg ttgtctttcc aacagccggc ggtgctgcca taaatacaat
     1201 gatccgttcc tttttttctt tttgtaactg ctgtaattgt tttaataaag gaagaaaaac
     1261 tttctggatc gtgtcttcgt tatagaccgc tgtcatttca aatccattga ctttaaataa
     1321 atattctttc cattctttca tatgacacct ctttctttat gtttattata cgatgtgttc
     1381 ttatacaagt acaagcaata gaccttttac tttggcagca ttaaaaagtg ccatcttttt
     1441 ctgttacaaa aaagatggca cttttgatct gtgacaaata tttaattaaa ttctgtgtaa
     1501 tgaatgaggt ctcccaaata atcatatcca aactggtaca ccagtatatt tctgatcacc
     1561 gcatatccaa agaaaataca cactcccaaa atcaacatcc acaatctcgg cttcatgctc
     1621 ttcatctctc tggctgtcag tttctttacc atccatcctg caatacacca tagaaacata
     1681 accccggtat atacaacggt tggattacat aaaaatgctc tctcaatatc caaatgcagc
     1741 agtgcgatca cacttcttgt cccgccgcat gccggacaat agaaatgtgt tacttcctta
     1801 aagaagcatg gcatacttct tatgatctta tatagagtca tcattttcat cgatatatgt
     1861 atcataatgt gtattaaact ctttcatttc attttgaaaa tccgaactaa gcaaaatatt
     1921 cacaacaaaa actgtgatca agatcacaga accaatgatg cctataatcc cacagatcaa
     1981 tcctgctttc gccttatttc ctcttgttcc tctgactttt ggtgtcaccg caaaacaaat
     2041 gaccgctgca atcccagtga taattgcgat tggcgtgcag caacaactga ccatgcatac
     2101 aactgctatg attccaagga tcattgttgc gatcgcaatt ccacttgtct gctcctgctg
     2161 tggttgttct atgtgatcat cttcgtaacg atatcgtctg tatggatcat catcttgtgt
     2221 atgatattct tgtgttggat cttcatcttt aatctcgtat ccagtatacg gatcgtatct
     2281 cttatcttcc atattttttc ctctactttc taatgtagcg aatcattcca ggccctaatt
     2341 tgtccgttgg acgagaaata aattcatgtt tgtgataaaa tggttctctt ccgatcgcac
     2401 acatcagatc cagcatgatc tcggttcctt cttcacaaag attctctaca tactcgatca
     2461 gatcttctat gattgcactt ccgattccat aatgctgata atctggatga atcattaaat
     2521 cctgaatgta acaaatgacc gccccgtcac ctacaagtcg tcccattcca acgatcttgg
     2581 catcaatgta tgcgcagacg atatacaaac tgttgtctaa ggctttcctc gcctgtgttc
     2641 ttgataatgg tttaaagttg accgcttttc ttaattccag atacgtatca actcctacat
     2701 tatttgatac tagtgttact aatttcattc tttccttgtc ttctttcttc tatataatat
     2761 gtttctcttc ctaatgtcaa aattatttct ctgattggct atcatcttat cacgaatttt
     2821 atcacattac aagaaatgaa tctttcttat tatattttat aattttatac acaataaaac
     2881 aggtgatttt tatttttttc cacaaaaatc acctgtttct tctatataat agtagatatt
     2941 ttaagaacta atcctgtaat gcatctacat cacgttctcc ggtacggatc ttaactacat
     3001 tccttacttc gtaactgaag atcttcccgt caccaacttc tcctgtaaat gcagatttct
     3061 gaatagcttc gatcgttttt ctttcccatt cttcagaaga tacgacgatc tcaaatttaa
     3121 tctttggacg catcatcata tcaacttttg ttccacggat aacttctgta aaacctttct
     3181 gtgctccgca tcccataacc tgtgatactg tgattccatt aactccgatt ttatttaaag
     3241 cttctttgac atcttcgaat ttctcttctc gaacgattgc attaattttg atcaacatat
     3301 attttcacct cttttaatct aaaccattaa aacttgggta agcattctct ccgtgttctg
     3361 atacatccaa tccgactttc tcggctcttt cttcaactct taatggagta aagatacgga
     3421 caatcgcgat acagatcagg cttccgacta ctgcaactgc gatagataca agaatcccta
     3481 tgatctgtgc tacaaacaat cttgtttctc caaacactaa tccattccat tttgcgactc
     3541 cattgatgga tgtcattcca aataatcctg ttgcgatacc accccagata ccaccgattc
     3601 cgtgacatcc aaatgcatcc aatgcatcat caatctttaa ttttcctttg atcagtttta
     3661 caccgaagta gcagattggg cttactaata atccacagat cactgctgcc cacattggaa
     3721 caaatccggc tcccggtgtg attgctacca gtccgataac taatcctgtg gaagctccga
     3781 ctaaagttgt ttttccttct gaaaatactt cgatcaacat ccatgaaaca agagctgcgg
     3841 ctgctgaaac agatgttgtc ataaatgcat gtgctgcaag tccatttgct gcaagagcac
     3901 ttcctgcatt gaatccgtac catccaaacc ataacagtgc agctcctaaa aatacaaatg
     3961 gtgtattatg tacgtggtag actccctggt catatccann nnnnnnnngt taaagctaat
     4021 accagaccgc tgacccctga tgtgatatga acaacatttc ctcctgcgaa atcgacagac
     4081 ccgatctctg ctaagaatcc tccttctccc cataccatat gtgccattgg gtagtacacg
     4141 atcaatgacc ataagataat aaaaagaact aatgctttaa atttcattct tcctgcaaca
     4201 gatcctgtga tcagtgctgg tgtgatcatt gcaaacatca tctgaaatgc tgcaaatgca
     4261 aggtttggaa tcgtatctgc atatggtcct ggtttcattc caactccgtt aaagccaaac
     4321 cattttgctc ctccaatgat ccctgcatgg tttccaccga aagatagtgc atatccaaca
     4381 agtacccaca tgatcacgga aactcccatg ataaatacac actgcatcat cgtgttccca
     4441 acgtttttcc ttcgtaccat tcctccatag aaaaatgcaa ggcctggtgt cataataaat
     4501 acaaatgctg cacatagcag aataaatgct gtatctcctg cgttcatagt atattcctcc
     4561 attctcattt tcaactatgc ttctatgctc gtcagagtag ctggttctga cattattgaa
     4621 aatctctcga gagaatgttt ttattaacta attctagcta ataaaaaagg agccaaagcn
     4681 nnnnnnnnnt tttctttatg ctttgactcc ttcgtcacgt ttatagtaac aaaaggtgtt
     4741 ttaaaatgca agatttattt atgtattttt atcaagacaa ttgtgatttg aatgacggaa
     4801 attattggtg gaatattata aaaagagatc atgtgtctgc aacccact
//
LOCUS       DAOMUE010000143         4846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4888_length_4846_cov_2.679608, whole genome shotgun sequence.
ACCESSION   DAOMUE010000143 DAOMUE010000000
VERSION     DAOMUE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4888_length_4846_cov_2.679608"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..381)
                     /locus_tag="PLC71_07100"
     CDS             complement(133..381)
                     /locus_tag="PLC71_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02445.1"
                     /translation="MKTLNKFIFLFLLSFISIGVFSGSIVSAQNTSSEPSIAVSEEDS
                     SVYSGDVIVWKYKTMNGKLYKRKYNKTTKKWIGNWIPA"
     gene            complement(365..1537)
                     /locus_tag="PLC71_07105"
     CDS             complement(365..1537)
                     /locus_tag="PLC71_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRM02446.1"
                     /translation="MTPANYGSVFSCCLFGSLMMLYLYVFCRSKRFILRNGTFVIYFA
                     VGVVTIRMLLPWNFHFAANVESHKILPFLFDLLRVKILSVEFLTILVIIFCFGSCLRL
                     ALYFYKLVRYRHLLHQLDPLDDIRILRIFSDILEEYHCTKQILIYQVPGLSSPAISGL
                     IHPVILLPDREYSDQDLRFIFMHELNHYLHHDLWKIFFWELVVCIYWWNPLSYMIRRL
                     IKDTAEYANDIRLTKDRDELTRTNYMLSLLNTSKQTHTFHTLPTLHFQEGTILNIEKR
                     CDLISDSPKIRRNLFSQFIHIGSISLLFTLSLCFIFQPSSPAPNYDNDGTVIFDKPDS
                     SNSFYIKRKDGKGYDLYVKHEDAFINTGIIENPEDYKSPKIYSSKKEATKFYENIK"
     gene            complement(1539..1925)
                     /locus_tag="PLC71_07110"
     CDS             complement(1539..1925)
                     /locus_tag="PLC71_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRM02447.1"
                     /translation="MKYKKLTPSQLNIMKTLWDKKEPMIASDFVQLDPSLNLNSVQSA
                     LRSLLKKNYIEVSDIVYSGKVLTRRYIPVVSSEDYASENINGVLEDLLSSNILLQYVE
                     SENDIKVIERLQEKLEERKKILERKS"
     gene            2252..3013
                     /locus_tag="PLC71_07115"
     CDS             2252..3013
                     /locus_tag="PLC71_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM02448.1"
                     /translation="MAGVILDAGHGGYDNGAQYDGRKEKDDNLRLTLAVGQILEQNGV
                     PVSYTRQSDIYQSPSEKARLGNESGADYFISFHRNASPNANEYQGVETLVFDDQGIKA
                     EMARNVNANLEQVGFRNIGVKERPNLTVLRRTKIPAILIEAGFLNSDQDNERFDEKFQ
                     EIAQAIADGIMETIGDENEALKGIYRVQIGLYRNFSNAQYVLNRAVADGYEGNIVYKD
                     PYYAVQIGEYNTLDEAVRLENELKKKGYDTLVIKE"
     gene            3378..4235
                     /locus_tag="PLC71_07120"
     CDS             3378..4235
                     /locus_tag="PLC71_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM02449.1"
                     /translation="MKIDKVMNNNVVSSIDEDGQEIIVVGTGIGFQGKEGKVVDEKKI
                     QKIFRLEDPKMIRKLKEILQDLPMEQFEISTAIIEHAKQSLGTELNENIYVTLTDHIH
                     FAIQRYEDHMNFPNPMLREVRLFYEKEFALGEYALGMIKQRLNISLPLDDAASIALHI
                     VSAEFDTRVKDTLKITEFLEDVMEQIKNYFHLEIDTQSLSYERFITHLKFLSRKLFAS
                     ERMDDMNNDVQEMIQKICPEEYQCAGYIKTFIKERYKKDMTSAEVAYLTMHIERIRKS
                     SIEKGEEDV"
     gene            4228..4725
                     /locus_tag="PLC71_07125"
     CDS             4228..4725
                     /locus_tag="PLC71_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HRM02450.1"
                     /translation="MFDSLKKMFEKNAKTISLKAVEDGRTIPMDEVNDQTFAQELLGP
                     GIAIVPSNGTVVSPINGTIATVMDTKHAVCIQGEDGLELIVHAGLDTVELNGKYYQTY
                     KEIGDQVKAGDVLLEFDLEEITKAGYDVTTPIVITNLGDYKITKCLTGQQVKAGEEVI
                     QLTKQ"
BASE COUNT         1806 a          699 c          964 g         1377 t
ORIGIN      
        1 tatttgatgt gatgatcgat agctgtgggg cattttgtgg aattatggta tctttgatca
       61 cgcagaaatt gatagagaaa aagaaaggaa atcagattta agatgtctga tttcctttct
      121 ttttcatgct cactaagctg gaatccaatt cccgatccat ttttttgttg ttttgttata
      181 ttttcttttg tataatttcc cgttcatagt cttatatttc caaacgatga catcgccgct
      241 gtatacagag gaatcttctt ctgaaacagc aatggaaggt tcgcttgagg tattttgggc
      301 tgagacgatt gaaccagaaa atacaccaat agagataaaa ctaagtaaaa atagaaaaat
      361 aaatttattt aatgttttca taaaatttcg tagcctcctt tttgctagaa tatattttag
      421 gacttttata atcttcaggg ttttcaatga ttcctgtatt tataaaagca tcttcatgtt
      481 tgacatataa atcatagccc tttccatctt ttcgctttat ataaaaagaa ttactgctgt
      541 caggtttatc aaaaataaca gtgccatcat tgtcatagtt tggagctggc gaacttggct
      601 gaaagataaa gcacagcgaa agggtgaaca ataaagaaat agatccaata tgtataaatt
      661 gtgaaaataa atttctgcga atctttggac tatcactgat aagatcgcat cttttctcaa
      721 tgtttaaaat cgtaccttcc tgaaaatgca gtgttggaag ggtgtggaac gtatgagtct
      781 gtttggaagt attgagaaga cttaacatat aatttgttct tgtaagttca tctctgtctt
      841 tggtaagtcg gatatcattt gcatattccg cagtatcttt gatcagacgg cgaatcatat
      901 aagaaagagg attccaccag tagatacata caacgagttc ccagaaaaaa atcttccata
      961 agtcatggtg tagataatga ttgagttcat gcataaaaat aaaacgcaga tcctgatcac
     1021 tgtattctct gtcaggaaga aggatcactg gatggatcag accagaaatt gcaggactag
     1081 aaaggccagg cacctgatag attaaaatct gcttcgtaca atgatattct tccaaaatat
     1141 cagaaaagat tcttaatatt cttatgtcat cgagagggtc taactgatga agtaaatgac
     1201 gatagcgaac aagtttatag aaatataaag caagtcttaa acaacttcca aagcaaaaaa
     1261 tgatcacaag gattgtcaga aattccacag ataaaatctt cacccttaaa agatcaaaaa
     1321 gaaaaggaag aattttatgg gattcaacat ttgctgcaaa atgaaaattc caaggcaaaa
     1381 gcatacggat cgtgacaact ccgacagcaa aataaatcac aaaggttccg tttcttaaga
     1441 taaatcgttt gcttctgcaa aatacataaa gataaagcat catcagactg ccaaaaagac
     1501 agcaactaaa tacggaacca taattcgcag gtgtcatatc aagacttcct ttctagaatt
     1561 ttcttacgtt cctctaattt ctcctgtaaa cgttcgatca ctttaatatc attttcagac
     1621 tccacatatt gaagaagtat attagaagat aaaagatctt ccagcactcc gttgatattc
     1681 tcagaagcat aatcttcaga ggaaacaaca ggaatataac gtctggttaa aaccttacca
     1741 ctatatacaa tgtcggaaac ttcaatatag tttttcttaa gtagagaacg caatgcagat
     1801 tgaacggaat tcagattcag tgaaggatca agctgaacaa aatcagaagc gatcataggc
     1861 tcttttttgt cccataatgt tttcatgata tttaattgtg atggtgttag ttttttgtat
     1921 ttcataaatt atcaactcct tgagtttatt ttatatgaat atatttatat tttcaacaac
     1981 tattttgaat taaaaaaaat aaatgtttta aattaaaata gttgttgaaa aaagaatcat
     2041 aatatagtag aatagaaaac gaagataata aatattgata catggtaggt ggggtattat
     2101 taaatttgta ataatacccc taaatttttt gtcaagtaat aggtcatatt ttcaataaaa
     2161 ctctttatca tcaggttatc tactaccaat ataacacaaa acatggagaa atgaatagtc
     2221 tgatagaaga aagaaataca gggaaaataa tatggcagga gtaattttag atgctggtca
     2281 cggcggttat gataatggag cccagtatga tggaagaaag gaaaaagacg acaatctaag
     2341 actgacactc gcagtcggtc agattttaga gcaaaatggc gttccagtat cctacacaag
     2401 acaaagtgat atctaccagt caccatctga aaaagcaagg cttgggaatg aaagtggagc
     2461 tgattatttt atctcatttc atagaaatgc aagtccaaat gcgaatgaat atcaaggcgt
     2521 tgaaacactg gtatttgatg atcaggggat caaagcagag atggcaagaa atgtaaatgc
     2581 gaacttagaa caagtaggat ttcgtaatat tggagttaaa gagcgcccga atcttactgt
     2641 attaagaaga acaaagattc ctgcgatctt gatcgaggca ggttttttaa attcagatca
     2701 ggacaacgaa agatttgatg agaaatttca agagatcgca caagcgatcg cagatggaat
     2761 tatggaaacg atcggagatg aaaatgaagc tctgaaggga atctatcgtg tccagattgg
     2821 tttataccgt aatttttcta atgcacagta tgttctgaat cgtgcggttg cagatggata
     2881 cgaggggaat atcgtttata aggatcctta ttatgcagtt cagattgggg aatataatac
     2941 gcttgatgaa gcagtaagat tggaaaatga attgaagaaa aaaggctatg atacacttgt
     3001 tatcaaggaa tgatcttaat ttaagaaaaa cacacatact gcaaggtgcg cagccaatag
     3061 agattgtaaa agatggcacg caccggcagt aaaaaagtat cacaatatct attgtaaaaa
     3121 atataaaaag aaaaagcctc aaaaggcttg acttttgtat aaaatgctga taagataaaa
     3181 gcaacttaag aaaataaagt gacttaaatg atatgaatat tgaggattgt cactggtaat
     3241 gcaggcaaaa ccggatttta taagtgtgaa agtgttgact ttcatattta taaaatgtgg
     3301 ttttcttttt gttttaaaat agaacatata atatatatac tagaagaaaa gcttccaatg
     3361 atcatggaga gaagattatg aaaatagata aggtaatgaa caacaacgta gtaagcagca
     3421 tcgatgaaga tggacaggaa atcattgttg ttggaacagg aattggattt caggggaagg
     3481 aaggaaaagt agttgatgag aagaagattc agaagatttt tcgattagaa gatccgaaga
     3541 tgatccggaa attaaaagaa atcttacaag atcttccgat ggaacaattt gagatttcaa
     3601 cagcgatcat agaacatgca aagcaaagtt taggaacaga attaaacgag aatatttatg
     3661 tgacattaac agatcatatt cattttgcga ttcagcgata tgaagatcat atgaattttc
     3721 caaacccgat gcttagggaa gtaagattat tttatgagaa agaatttgca cttggagaat
     3781 acgcgttagg aatgatcaaa cagcgattga atatatcact cccattagat gatgcggcat
     3841 ccattgcttt acacatcgtg agtgcagaat ttgatacaag agtcaaagat actttaaaga
     3901 tcactgaatt tttagaagat gttatggaac agataaagaa ctacttccat ttggagatcg
     3961 atacccagtc attgagttat gaacggttta tcacacattt gaagttttta tccagaaagt
     4021 tatttgcgtc agaacgaatg gatgatatga acaatgatgt acaggagatg atccagaaga
     4081 tctgtccgga agaatatcag tgtgcaggat atattaagac ttttattaaa gaaagataca
     4141 aaaaagatat gacatcagca gaagttgcat atttaacgat gcatatcgag aggatcagaa
     4201 aaagcagtat agaaaaagga gaagaagatg tttgattcat taaagaagat gtttgagaag
     4261 aacgcaaaga caatttcatt aaaagcagta gaagatggga gaacaatccc aatggacgaa
     4321 gtcaatgatc agacattcgc tcaggaatta ttaggaccag gaatcgcaat tgttccatcc
     4381 aatggaacag tcgtttctcc gatcaatgga acgatcgcca cggtaatgga tacaaaacat
     4441 gcagtctgta ttcagggaga agacggatta gagctgatcg ttcatgcagg acttgatacc
     4501 gtggaattaa atggaaagta ctatcagaca tataaagaaa ttggagatca ggtaaaggca
     4561 ggagacgttt tgttggaatt tgatcttgaa gagattacaa aggcgggata tgacgtcaca
     4621 acgccgatcg taataacgaa cttaggtgat tataagatca cgaagtgttt gacaggacaa
     4681 caagtaaaag caggggaaga ggtgatacaa ttaacaaaac aataacaaag atgagtaact
     4741 aaaagagaag gaaaagaaca tgaaaaactt aaagtaaaaa tgaaagggga caatctatat
     4801 gaaattttta caaaaattag gaaaagcgtt aatgcttcca gtagca
//
LOCUS       DAOMUE010000144         4810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4931_length_4810_cov_2.441220, whole genome shotgun sequence.
ACCESSION   DAOMUE010000144 DAOMUE010000000
VERSION     DAOMUE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4931_length_4810_cov_2.441220"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /gene="hisD"
                     /locus_tag="PLC71_07130"
     CDS             <1..1023
                     /gene="hisD"
                     /locus_tag="PLC71_07130"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRM02451.1"
                     /translation="RDFHEKQIQKSWFETREDGVMLGQKVTPMETCGVYVPGGKAVYP
                     SSVLMNIVPAHVAGVKNIIMTTPPGKDGKVTANTLVAAKEAGATKVYKVGGAQAIAAM
                     AFGTESIPKVDKIVGPGNIFVALAKKAVYGNVSIDSIAGPSEILVLADETANPRYVAA
                     DLLSQAEHDEMASAILVTTSEELAHKVSDEIDGFLNQLSRKEIMEKSLDSFGYILVAS
                     DMKEAIDTANAIASEHMEIMTANPFDVMTRIKNAGAIFIGAYSCEPLGDYFAGPNHVL
                     PTNGTAKFFSPLSVDDFIKKSSVIYYSREALEPVHKDIEFFANQEQLTAHANSMKVRF
                     EDNQEG"
     gene            1028..1615
                     /gene="hisB"
                     /locus_tag="PLC71_07135"
     CDS             1028..1615
                     /gene="hisB"
                     /locus_tag="PLC71_07135"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRM02452.1"
                     /translation="MSRSASIERNTSETKIKLSIDLDGNGNGNIHTGIGFFDHMLNAF
                     ARHGFFDLDVEVEGDLYVDCHHTIEDVGIVLGEAIKQAVGDKKGIKRYGSFMLPMDET
                     LMLCAIDLSGRPYFVMDCDFTVDRVGEFDTEMVREFFYAVSYGSMMNLHLKKLHGENN
                     HHMIEAAFKAFAKALDEATSMDPRITDVLSTKGAL"
     gene            1650..1973
                     /gene="hisI"
                     /locus_tag="PLC71_07140"
                     /pseudo
     CDS             1650..1973
                     /gene="hisI"
                     /locus_tag="PLC71_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567179.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
     gene            2062..2184
                     /locus_tag="PLC71_07145"
     CDS             2062..2184
                     /locus_tag="PLC71_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02453.1"
                     /translation="MTAFLSELVSYVLKFAILFACSAAGIYFGVGRAKAKKKES"
     gene            2305..>4810
                     /gene="alaS"
                     /locus_tag="PLC71_07150"
     CDS             2305..>4810
                     /gene="alaS"
                     /locus_tag="PLC71_07150"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRM02454.1"
                     /translation="MRAYGVNELRKMYLEFFESKGHLALKSFSLVPKNDNSLLLINAG
                     MAPLKPYFTGQEVPPRTRVTTCQKCIRTGDIENVGKTARHGTFFEMLGNFSFGDYFKH
                     EAIAWSWEFLTKVIGLDPDRLYPSVYEDDDEAFEIWEKEIGIAPERIFRFGKEDNFWE
                     HGAGPCGPCSEIYYDRGEKYGCGSPDCTVGCDCDRYMEVWNNVFTQFENDGHNHYTEL
                     ENKNIDTGMGLERLAVVVQEVDSIFDVDSIKAIRDEICKVAGVTYGADHETDVSIRVI
                     TDHIRSATFMLSDGITPSNEGRGYVLRRLIRRAARHGRLLGIDHLFLTDISKVVIRES
                     KDGYQELEEKAEFIFNHLSQEEKQFNKTIDQGLSILADMEKAMNEKGSKELAGADAFK
                     LYDTYGFPLDLTIEILEEKGFTVDKEGFEKEMQVQRETARAARKTTNYMGADATVYEQ
                     LDPAMTTKFVGYDELTCEGEITAMTTETEVVSTLNKGDKGTIVADQTAFYATSGGQEA
                     DKGVIISGDASFEVEDVIKLSGGKFGHVGHVVEGTFNVGDKAVFTVNEKNRALGAKNH
                     SATHLLQKALREVLGTHVEQAGSLNNAEHLRFDFTHFSPLSDDEIARVEKIVNEKIAQ
                     DLPVVTKVMSMEEAKKTGAMALFGEKYGDEVRVVSMGDFSVELCGGTHVKNTGAITAF
                     KILSETGVASGVRRIEAVTDQGVFNYYHKQEEELKEAAKALKATPATLLTRIESLLAE
                     VKELKSENESLKSKLAKDALGDVMDQVEEVKGVKLLATSIEDVDMNGLRELGDNLKEK
                     LGEGVIVLASQKDGKVFLVAMVTDEAQKAGAHAGNLI"
BASE COUNT         1672 a          782 c         1134 g         1222 t
ORIGIN      
        1 cgtgatttcc atgaaaaaca gattcagaag agctggtttg agacaagaga agacggagta
       61 atgcttggac agaaagtaac accaatggaa acttgtggtg tctatgtacc aggtggaaaa
      121 gcagtttatc catcttctgt tttaatgaac attgttccag cacacgttgc aggagtgaaa
      181 aatatcatca tgacaacacc tcctggaaaa gacggaaaag ttacagcaaa tacattagtt
      241 gcagcgaaag aagctggagc aacaaaagta tataaagtcg gtggagcaca ggcaattgca
      301 gcgatggcat ttggaacaga gtctattcca aaagtagaca agatcgtagg acctggtaac
      361 atttttgtgg ctcttgccaa gaaagcagtt tatggaaacg taagtatcga ttccattgca
      421 ggaccaagtg agatcttagt gttagctgat gaaacagcga atccaagata tgtggcagca
      481 gacttacttt cccaggcaga acatgatgag atggcaagtg cgatccttgt tacaacaagc
      541 gaagaattag cacataaagt atctgatgag attgacggat tcttaaatca gttatcaaga
      601 aaagagatca tggagaaatc tttagattcc ttcggatata ttcttgttgc aagcgatatg
      661 aaagaagcaa ttgatacggc aaacgctatt gcgtctgaac atatggagat catgacagca
      721 aatccatttg atgttatgac aaggatcaag aatgcaggag cgatctttat cggagcatac
      781 agctgtgaac ctttaggaga ttattttgca ggaccaaacc acgtacttcc aacaaatgga
      841 acagcaaaat tcttctctcc attaagtgtt gatgatttca tcaagaaatc aagtgtcatc
      901 tactattcaa gagaagcctt agagccagta cacaaagaca tcgaattctt tgcgaatcag
      961 gaacagctga cagcacatgc taattccatg aaagtaagat ttgaagataa ccaggaagga
     1021 taaaaatatg agtagaagtg catcaattga gagaaataca agtgagacaa agatcaaact
     1081 ttccattgat cttgatggaa atggaaatgg aaatatccat acaggaatcg gtttttttga
     1141 ccatatgtta aatgcatttg cacgtcacgg attttttgat cttgatgtgg aagttgaggg
     1201 agatctctat gtagactgcc atcatacgat cgaagatgtc ggaatcgtac ttggagaagc
     1261 gatcaaacag gccgttggag ataagaaagg gatcaaacgt tatggatcat ttatgcttcc
     1321 aatggatgag acattaatgt tatgtgcgat cgatctgtct ggaagaccat attttgtgat
     1381 ggattgtgat tttacggttg atcgcgttgg agaatttgac acagagatgg taagagaatt
     1441 cttttatgca gtatcttacg gcagcatgat gaatcttcat ttgaagaaat tacatggaga
     1501 gaataatcat catatgatcg aagcagcatt taaagcattt gcaaaagctt tggatgaagc
     1561 aaccagcatg gatccaagga tcacagatgt actttccacg aaaggagcct tataagatga
     1621 gtaatgaggc aaaaccatta aatagcaaga tgagttttga tgaattcaaa ttaaacagcg
     1681 acggaatgtt aaccgttgtt gtacaggatc ataagacaaa tgaagttttg atggtcgcat
     1741 atatgacaaa agaagcatat gataagacga tcgagacagg gattatgact tattacagcc
     1801 gaagccgtga tgaattatgg atcaaagggg agacatccgg acattatcag tatgtcaaag
     1861 atttatatat tgactgcgac agagacacct tacttgctaa ggtaaaacag atcggggctg
     1921 cctgccatac aggaaattat tcttgcttct acacagattt attagaaaaa gaagattaag
     1981 aacgtaatct ttgtgagaaa atatttgtgt ttctaagttc gatttggtat aatcgacttt
     2041 ataagataag gaggcaagat tatgacagca tttttatcag aattagtctc ttatgtattg
     2101 aagtttgcga ttctttttgc atgctctgcg gcaggaattt atttcggagt cggtcgtgcg
     2161 aaggcaaaga agaaagaatc ataagatcat acatgattaa gagaagatca tttagctgca
     2221 tgagaataca ggctcatgca gctaaaaagc cgtaaaggca aggtcttctt aaatataatg
     2281 aaacgacact aggaggatga aacgttgaga gcatatggag tcaacgaact caggaagatg
     2341 tatcttgagt ttttcgaaag taaaggacat ttagcattaa agagtttttc actcgtgcca
     2401 aagaacgata acagtttatt actgatcaac gctggtatgg caccactgaa accttatttc
     2461 acaggacagg aagtaccacc aagaacaaga gtgacaacat gtcagaagtg tatccgtaca
     2521 ggagatattg agaatgttgg taagacagca agacatggta cattctttga gatgttagga
     2581 aacttctctt ttggagatta cttcaaacat gaagcaatcg catggtcttg ggaattttta
     2641 acaaaagtga tcggattaga tcctgacaga ttatatccat ctgtatacga agatgatgat
     2701 gaagcattcg agatctggga gaaagagatc ggaatcgcac cagagagaat cttccgtttt
     2761 ggaaaagaag acaacttctg ggagcatggg gcaggacctt gtggaccatg ttcagaaatc
     2821 tactatgacc gtggagagaa atatggatgt ggaagcccag attgtacggt aggatgtgac
     2881 tgcgaccgtt atatggaagt atggaacaac gtatttacac agtttgagaa tgatggacac
     2941 aaccattata cagaattaga gaataagaac attgatacag gtatgggact ggaacgtctt
     3001 gcagtcgttg tacaggaagt agattctatc ttcgacgtag attctatcaa agcgatccgc
     3061 gatgagatct gtaaagttgc aggagtcaca tatggtgcag accacgagac agatgtatcg
     3121 atccgtgtaa tcacagacca tatccgttct gctacattta tgttgtctga tggtatcaca
     3181 ccatccaacg aaggacgtgg atatgtactt cgccgtttga tccgccgtgc agcaagacat
     3241 ggaagattat taggaatcga tcacctgttc ttaacagata tcagtaaagt cgtgatccgt
     3301 gaatccaagg atggatatca agaattagaa gagaaagcag aatttatctt caatcattta
     3361 tctcaggaag aaaaacagtt caacaagacg atcgatcagg gactgtctat cttagcagat
     3421 atggaaaaag ctatgaacga gaaaggcagc aaagaacttg ctggtgcaga tgcgttcaaa
     3481 ttatatgaca catatggatt cccattagat ctgacgatcg agatcttaga agaaaaggga
     3541 tttactgtag ataaagaagg ctttgaaaag gaaatgcagg ttcagagaga gactgccaga
     3601 gctgcaagaa agacaacaaa ctacatgggt gcagatgcaa ctgtatacga acagttagat
     3661 ccagccatga caacaaaatt cgttggatat gatgaactta cttgtgaagg tgagatcaca
     3721 gctatgacaa cagagacaga agttgtttca actctgaaca aaggagacaa aggaacgatc
     3781 gtcgctgacc agacagcatt ctatgctaca agcggtggtc aggaagcaga taaaggtgtg
     3841 atcatcagcg gagatgcatc ttttgaagta gaagatgtca ttaaattatc cggtggtaag
     3901 tttggacatg ttggacatgt agtcgaagga acattcaacg taggagacaa agcggtcttt
     3961 acagtgaatg agaagaacag agcattaggt gcaaagaacc atagtgcaac tcatttatta
     4021 cagaaagcat taagagaagt gcttggaact catgtagagc aggcaggatc tttaaataat
     4081 gcggaacatt taagattcga ctttacacat ttctcaccat tatctgatga tgagatcgca
     4141 agagttgaga agatcgtcaa cgaaaagatc gctcaggatc ttccagttgt gacaaaagtt
     4201 atgtctatgg aagaagctaa gaaaacagga gctatggcac tgtttggaga gaaatacgga
     4261 gacgaagtac gcgttgtatc catgggagat ttctctgtag aattatgtgg tggtactcat
     4321 gtgaagaata caggagcgat cacagcattc aagattttat ctgaaactgg tgttgcatca
     4381 ggagttcgaa gaatcgaagc agtgacagat cagggagtat ttaactacta ccataagcag
     4441 gaagaagaat taaaagaagc agccaaagca ttaaaagcta caccagcaac actgctgaca
     4501 agaattgaat cacttcttgc agaagtaaaa gaattaaaga gcgaaaacga atccttaaag
     4561 agcaaacttg caaaagacgc tctgggagat gttatggatc aggtagaaga agtaaaagga
     4621 gtgaaacttc ttgcaacttc tatagaagac gttgatatga atggacttcg tgaacttgga
     4681 gataacttaa aagagaaatt aggagaaggt gtcattgtcc ttgcttccca gaaagacgga
     4741 aaagtattct tagttgccat ggtaacagac gaggcacaga aagcaggagc acacgctgga
     4801 aacctgatca
//
LOCUS       DAOMUE010000145         4808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4938_length_4808_cov_2.808963, whole genome shotgun sequence.
ACCESSION   DAOMUE010000145 DAOMUE010000000
VERSION     DAOMUE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4938_length_4808_cov_2.808963"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..974
                     /locus_tag="PLC71_07155"
     CDS             216..974
                     /locus_tag="PLC71_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRM02455.1"
                     /translation="MEMKKVLKYAMEIGECMLVSGAGVSRVEDTISRICKAYGAKEAN
                     VFSITSSIVTTIEDEKGEILTQTKRIRSYKTDFTKLDELNQLSRYMCKELPEEEEVKR
                     KISEIKKGKVVVFTEEVLTSAVIAAAWTSFYGGGITEGIIAMIAGAFVAVLARYIKIL
                     APNEMVLNFLLSFFVAAFAYISAKFGLTEDYGKIIIGNIMLLVPGVAFVNALRDMIGG
                     DMMSGILRVCESILLAVFLAAGSFMALMFLGGVL"
     gene            975..1427
                     /locus_tag="PLC71_07160"
     CDS             975..1427
                     /locus_tag="PLC71_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRM02456.1"
                     /translation="MKAEIIQVLASFVGSFGFAVLYNLRGKKLCMAGISGMVSWIAYL
                     IVWNEMPSTFVANLAAAAVATIYAETMARILKTPVTVFLITGIIPLVPGGNLYYTMNY
                     VLAKQWHLFYLYGQKTLLIAVAVAAGIMTASSVYGICASVWKYCKKEI"
     gene            1494..3194
                     /locus_tag="PLC71_07165"
     CDS             1494..3194
                     /locus_tag="PLC71_07165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRM02457.1"
                     /translation="MEQDEKFENQLNLALDLSEEERERSKDLDAGYDKETDQWEIIFR
                     YAGTLDTIRWSDTTRIEPLSMGYAIAYIKEDELEAFGANEQVIYIEKPKNLLLSRQPS
                     IAASCMLSVKNPPLSLTGRGVFVAVIDTGIDIFHPDFIDENGDTKIYALWDQTVKGSP
                     PKPFLNGNLYTKEEINKVLHSESGRYDFRSRDRSGHGTHVASICAGINGVAPEAELIV
                     VKLGDTREQGFPRTTQLMTALQYVINEASQAGRPVAVNISYGHNYGDHRGNSLLERFI
                     SQIAQQWKCTICIGTGNEGNSGKHKQGKLIKEEQKILLDIAPFEQNLNLQIWKDFVDE
                     LRIQLESPSGISYEITDKQGKSQYAYGNTIVFVYNGYPTPYNVRQEIFLSFIVQEGNH
                     IESGQWNLTLIPRNIRNGEYQMWLPVSLGSNQETRFLEPDKEFTLTIPSTAEKVISAG
                     AYDVRYQSYADFSGRGDQRYGVKKPDLVAPGVGILAAAPGGGENSLSGTSMATPFVTG
                     AAALLMEWGIIRKNDPYLYGERIKAYFHKGARKMNGFLDYPNNEIGYGKLCVAASLYN
                     "
     gene            3306..>4808
                     /gene="glgP"
                     /locus_tag="PLC71_07170"
     CDS             3306..>4808
                     /gene="glgP"
                     /locus_tag="PLC71_07170"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HRM02458.1"
                     /translation="MKTERFDKKLFKQEVLNNLKTQFRVELDNASQQQIYQAVAYALK
                     EWIIEDWMDTQKTYEEKDPKFLYYMSMEFLMGRALGNNLINMSMYGEVKEALDELGVD
                     LNAVEDQEPDPALGNGGLGRLAACFLDSLATLGYAAYGCGIRYQYGMFKQKIKDGYQI
                     EVPDEWLKNGNPFELKRPEYAKEVRFGGNIRTEYDEATGRTNFIQENYQSVMAVPFDY
                     PIVGYGNHIVNTLRIWDAEAITDFQLDSFDKGEYDKAVEQKNLAKNIVEVLYPNDNHY
                     EGKELRLKQQYFFVSASLQAAVAKYKKNHDDITKLYEKMTIQMNDTHPTVSVAELMRI
                     LMDEEGLGWDEAWEVTTKTCAYTNHTIMAEALEKWPIDLFSKLLPRVYQIVEEIDRRF
                     VNKIREMYPGNEEKVKKMAILWDGQVRMAHMAIAAGYSVNGVAKLHTEILKNQELKDF
                     YQMMPEKFNNKTNGITQRRFLMHGNPLLADWVTKKLGTDTWATDLSLMSGL"
BASE COUNT         1659 a          764 c         1123 g         1262 t
ORIGIN      
        1 ctttaagtgt tgcatgtcat atcggtgcag gtgcattggc ggttgcttgg acacataaga
       61 ttgatgatta atagcattaa tcttcttttc atctcgaaga aatgtaaaca aaacatgaat
      121 tttacatgaa atccttgtgt gatcatagca caagggtttc ttttgtgtta caatacagtc
      181 aatttcgata caatagatag caggagatag atgttatgga aatgaaaaaa gtattaaaat
      241 acgcaatgga gatcggagaa tgcatgctag tgtcaggagc aggtgtttca agggtagaag
      301 atacgatttc aagaatctgc aaggcgtatg gagcaaaaga agccaatgta ttttctatca
      361 catcaagtat tgtaacaacg atcgaagatg aaaaaggaga aatcttaacg cagacaaagc
      421 gtatcaggtc atataagaca gactttacca aactggatga gttaaatcag ttatcaagat
      481 atatgtgcaa agaacttccg gaagaagagg aagtaaaacg aaagatttca gagattaaaa
      541 agggaaaagt cgtcgttttt acagaggaag ttcttacatc agcggtcatt gcagcagcat
      601 ggacttcatt ttatggaggt ggcattacag aaggaatcat agcaatgatc gcaggggcat
      661 ttgtcgcggt tcttgcaaga tatattaaga tcttagcacc caacgaaatg gtattaaact
      721 ttctattatc attttttgtt gcagcatttg catatatcag tgcaaaattc ggactgacgg
      781 aggattatgg aaagatcatc atcggtaata tcatgctgtt ggttcctgga gttgcatttg
      841 ttaatgcact tcgggatatg atcggtggag acatgatgtc aggaatctta agagtctgtg
      901 aatcgatact gttagctgta ttccttgcag caggttcttt tatggcattg atgttcttag
      961 gaggtgtgtt ataaatgaaa gcagagatca tacaggtact ggcaagtttt gtcggaagtt
     1021 ttggatttgc agtactttat aaccttcgag ggaaaaaact ttgtatggct gggatttctg
     1081 ggatggtatc atggatcgcc tatcttatcg tgtggaatga gatgccaagt acatttgtgg
     1141 cgaatctggc agcggccgca gttgcgacga tctatgcaga aacaatggca aggatcttaa
     1201 agactcctgt aacagtattt ttgatcacgg gaatcattcc attagtacca ggtggaaatt
     1261 tatactatac gatgaattat gtcttggcaa aacagtggca tttattttat ttatatgggc
     1321 aaaaaacatt gctgatcgca gtggcggttg cggcagggat catgacagca tcttctgttt
     1381 acggaatttg tgcatccgta tggaaatact gcaaaaaaga gatatgagca atctaagaat
     1441 aaacctcaca gattttgcat aagataagaa aaacgaaagt gtgcaggtat cgtatggaac
     1501 aggatgaaaa gtttgaaaat caattaaatc ttgctctgga tcttagcgaa gaagaacggg
     1561 agagatccaa agatttagat gcaggatacg ataaagaaac ggatcagtgg gagatcatat
     1621 ttcgctacgc aggcacactt gatacgatca gatggtcaga cacaacgagg atcgaaccgt
     1681 tgagtatggg ttatgcgatc gcctatataa aagaagacga gttggaagca tttggggcaa
     1741 atgaacaagt tatttatata gaaaaaccga aaaatctatt attaagccga cagccatcga
     1801 tagccgcatc ttgtatgttg tcggtcaaga atccacctct ttcattgact ggaagaggtg
     1861 tttttgtcgc tgtgatcgat acagggattg atatttttca cccggatttt atagatgaaa
     1921 atggagatac taagatttat gcactatggg atcaaacggt caaaggaagt ccgcctaaac
     1981 catttcttaa cggaaatctc tatacaaaag aagaaatcaa taaagtattg cactcagaaa
     2041 gtggaagata cgattttcgg agcagagatc gatctgggca tggaacacat gtagcttcaa
     2101 tctgtgccgg gataaacggg gttgcaccag aggcagaact gatcgttgtg aaattgggag
     2161 atacgagaga acaaggtttc ccaaggacga cacaattgat gacagcatta caatatgtga
     2221 tcaatgaagc atcacaggca ggacggccgg ttgcggtcaa tatcagttat ggacataatt
     2281 atggagatca cagaggaaat tccttgttag aaagatttat atcacagatc gcacagcaat
     2341 ggaaatgtac gatctgcatt ggaacaggaa atgagggaaa tagtggaaag cataaacaag
     2401 gaaagctgat caaagaagaa caaaaaatct tattggatat tgcaccattt gagcagaatc
     2461 taaatctcca gatatggaaa gatttcgtgg atgagttaag aatacagctg gaatctccat
     2521 cgggaatatc ttatgagatc acagataaac agggaaagtc acaatatgca tatggaaata
     2581 cgatcgtctt tgtatacaat ggttatccaa caccgtacaa tgtaaggcag gaaatattcc
     2641 ttagttttat tgtgcaggaa ggaaatcata tagagagtgg acaatggaat cttacattga
     2701 ttccacgaaa tatcagaaat ggggaatatc agatgtggct tccggtatcc ttaggaagta
     2761 atcaggaaac tagattttta gagccagata aagagtttac tttaacaatc ccatcaactg
     2821 cagaaaaagt gatctcagca ggtgcgtatg atgtcagata tcagtcctat gcagattttt
     2881 cggggcgggg tgatcaaaga tatggtgtaa agaaaccaga tcttgtggca cctggagttg
     2941 gaattttggc agcagcaccg ggaggcggtg aaaacagttt gtccgggacg tcaatggcaa
     3001 cgccatttgt cacaggagcg gcagctcttt tgatggaatg ggggatcata agaaaaaatg
     3061 atccttattt gtatggagag cggatcaaag cttattttca taaaggtgca aggaaaatga
     3121 atggattttt ggactatcca aacaatgaaa ttggatatgg aaaattatgc gtggcagcta
     3181 gtttgtataa ttagaagaac ttacgaaaaa aaatacaaat gatatttgta atttttgtat
     3241 catatttcca atttatatga taaaattaat aacacactgg gaacacgaaa ggggaagaga
     3301 caatcatgaa aacagaaaga tttgataaga aactgtttaa acaggaagta ctcaataatc
     3361 taaagactca gttcagagtt gagcttgata atgcatccca gcagcagatt tatcaggctg
     3421 tggcttatgc cctgaaagag tggatcattg aggactggat ggatactcag aagacctacg
     3481 aagagaaaga cccaaagttt ttatattata tgtccatgga attcctgatg ggaagagcac
     3541 ttggaaataa cctcattaac atgagtatgt atggagaagt gaaagaagca ctcgatgaat
     3601 taggcgtaga tctgaatgcc gttgaagacc aggaaccaga cccagcactt ggtaatggtg
     3661 gtcttggaag attagcagca tgtttccttg attcattggc aacacttgga tatgcagcat
     3721 atggatgcgg aatccgctat caatacggaa tgttcaaaca gaagatcaaa gacggatatc
     3781 agatagaagt accagatgaa tggttaaaga atggaaatcc atttgaatta aaacgtccgg
     3841 aatatgccaa agaagtacgt ttcggtggaa atatccgtac agaatatgat gaagcaactg
     3901 gaagaaccaa cttcattcag gagaactatc agtcagttat ggcagtccca tttgactatc
     3961 cgatcgtagg atatggaaat catatcgtta atacattaag gatctgggat gcagaagcga
     4021 tcactgactt ccagctggat tcttttgata aaggtgaata tgacaaagca gtagaacaga
     4081 agaaccttgc aaagaacatc gtagaagtat tatatccaaa cgataatcac tacgaaggaa
     4141 aagagcttcg tttaaaacag cagtatttct ttgtatctgc aagtttacag gcagcagtag
     4201 ccaaatataa gaaaaaccac gatgacatta caaaacttta cgagaagatg acgatccaga
     4261 tgaacgatac tcatccaaca gtatctgttg cagaacttat gagaatctta atggatgaag
     4321 aaggtcttgg atgggatgaa gcatgggaag ttacaacaaa gacttgtgca tacacaaatc
     4381 atacgatcat ggcagaagcg ttagagaaat ggccaatcga cttattctca aaattacttc
     4441 ctagagtata ccagatcgta gaagaaatcg accgtcgttt cgtaaacaag atccgtgaga
     4501 tgtatccagg aaacgaagag aaagtcaaga agatggcgat cctttgggat ggacaggtac
     4561 gtatggcaca tatggcgatc gcagcaggat attctgtcaa tggtgttgca aaacttcata
     4621 cagaaatctt aaagaatcag gaattaaaag atttctatca gatgatgcca gagaaattca
     4681 acaacaagac aaatggaatc acacagagac gtttcttaat gcatggaaac ccattacttg
     4741 cagattgggt aacaaagaaa cttggaacag acacatgggc aacagacctt tctttaatgt
     4801 caggactg
//
LOCUS       DAOMUE010000146         4792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4958_length_4792_cov_2.345788, whole genome shotgun sequence.
ACCESSION   DAOMUE010000146 DAOMUE010000000
VERSION     DAOMUE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4958_length_4792_cov_2.345788"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..696
                     /locus_tag="PLC71_07175"
     CDS             130..696
                     /locus_tag="PLC71_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRM02459.1"
                     /translation="MLFRRVKVITILLMVFMMSIGCSRRKEVSIEPKKETKTTAEKSS
                     SKETIYVYVCGEVKHPGVYRFSKGERIVSAVKAAGGLTKKASAESINQAEKMKDGQQI
                     TIPSKKQTAKNEGSDSKAVQSSSGKININTAGVKELMTLSGIGEAKASDIISYREEHG
                     PFSDISDIMKIQGIKEGIYHKIKDKITI"
     gene            713..1402
                     /locus_tag="PLC71_07180"
     CDS             713..1402
                     /locus_tag="PLC71_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM02460.1"
                     /translation="MRKVLVVDDEKLIVKGIKFSLEQDEMQVDCAYDGEEALEKAKEN
                     QYDIILLDVMLPGLTGFEVCQAIREFSSVPIIMLTAKGEDMDKILGLEYGADDYITKP
                     FNILEVKARIKAILRRSAHHDVKEEESAKVVECRGLKIDCESRRVHIHGKEVNLTAKE
                     FDLLELLVFHPNKVYSREDLLNTVWGYDYPGDARTVDVHIRRLREKIEVNPGVPDYVH
                     TKWGVGYYFQS"
     gene            1449..2831
                     /locus_tag="PLC71_07185"
     CDS             1449..2831
                     /locus_tag="PLC71_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM02461.1"
                     /translation="MSVFLLTTLISVLFSKLLNDGYRQKTYDQKVDSVVSQCSWLGDQ
                     VVGVDFHLDDGSNSLSNQIDELAASMNGRIIVIKNNYKIIKDTHTDFQSRFFLNDNVL
                     DVMDGKKTRIVSESGKYIEVMVPIVSKAGKQNHILGAVQATVPSEDMMGAVTEMEHRR
                     NMLLVACIICGLSMAMIISYLLTRDLKKIQRDVDFIAAGHEDESIEETGFLEVRSIVQ
                     RFNEILGRMQTLEDSRQEFVSNVSHELKTPMTSMKVLADSLIQQGDQVPAELYREFMQ
                     DIVAEIDRENVIISDLLSLVKLDKKAAQLQITTVSINELLEIIMKRLKPLALQRNIEL
                     IFESFRPVTAEIDEVKLSLGLTNLIENGIKYNKDDGWIRVTLNADHKYFYVKVADSGV
                     GIPEDCQDQVFDRFYRVDKARSRDTGGTGLGLAITKSVVSLHNGEIKLYSKVGEGTTF
                     TLRIPLKYKG"
     gene            2841..3359
                     /locus_tag="PLC71_07190"
     CDS             2841..3359
                     /locus_tag="PLC71_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRM02462.1"
                     /translation="MKKRKLCMIFLLCLALMTLVAGCRSKKETNDQGNQKGTAIYYTN
                     NDVTKLIMKKENVELEGNQQQKVKILLKKLQQTPKSDKIRSVIPKRIMINGVSVNTNI
                     VEIDFSTGYKRISENRDLICRAGIVYTLTQLKDINYVSFSISGEPMLDTDGTAIGALG
                     RDSFVFGKLPMK"
     gene            3363..>4792
                     /locus_tag="PLC71_07195"
     CDS             3363..>4792
                     /locus_tag="PLC71_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRM02463.1"
                     /translation="MKQRPMVLIFAGVLSGTAVVWNIMSMTAAFYLLTGICLLASLIF
                     SDRRYGWVVVGILAGILLSLGFKTSMKLDLIQVKEKATEYTMGRVLEVSKTKKGKLAV
                     LLKNKNLEGKFLLYTPEDEKIIPGDILRFQGELEEWEDAANPGQFSSRHYYFSKGIYY
                     HVYSKNIEIEGHQNIYFQEKILQCREYMKHQLEIQYKNEVSDFLKGMLIGDKNGLSDE
                     VKDDFKESGLIHLLAVSGLHISLAGRRVYKLVRKWCGNFVVGSLAGMTGAVFYCILTG
                     GSGSSLRAVMMLGVYFLSEILGEHYDMMSAGAFAGIVLLIMCPYRIYDTGFLYSFTAV
                     FVIAIYQLVKPKMKGKYYKLKESLSFCIFIQIGMLPLIIYFQYEAPAFSFLANVAAVP
                     LATCAFTLAFLLIFLPYTVFHEAISWMIQGVLWISRQSYGMLTIGHVPFLWVLLFYFM
                     TGLWIWKKNGQNRHIRISLAYVIMII"
BASE COUNT         1544 a          652 c         1153 g         1443 t
ORIGIN      
        1 tttgtttgtt gcaggtttgt taatatttaa atggaaatga taaatatctc ttgtaaagta
       61 ttataatttt cgctaaaata gaaaatagat ttacatgttc cagactcttt gcataagacc
      121 gggaggttta tgttgtttag aagagttaaa gtgataacca ttttgctgat ggtttttatg
      181 atgtccattg gatgcagccg caggaaggaa gtatcgatag aaccaaagaa ggagacaaag
      241 acaacggctg agaagtcttc ttctaaggaa actatttatg tctatgtatg tggagaagta
      301 aaacatccag gagtttatcg tttttcgaaa ggtgaacgta ttgtttctgc tgtgaaagca
      361 gctggtggac taactaagaa ggcttcagca gagagtatta atcaggcaga gaagatgaaa
      421 gacgggcagc agataacgat tccgtcaaag aaacagacag cgaagaatga aggatctgat
      481 tccaaagcag tgcaatcttc ctctggaaag atcaatatca atactgcagg tgtcaaggag
      541 ttgatgacgc tttcagggat aggggaagcg aaggcttctg atattatctc ttatcgagag
      601 gaacatggcc ctttttctga tatttcagat attatgaaga ttcagggaat taaagagggg
      661 atatatcata agatcaaaga caagataaca atataatttt agaggtgaaa aaatgaggaa
      721 ggtcttagtt gttgatgatg aaaaactaat cgtgaagggc atcaaattca gtctggaaca
      781 agatgagatg caagtagatt gtgcttatga tggagaagaa gcattagaga aagccaaaga
      841 gaatcaatat gatattattc ttcttgatgt aatgcttcca ggactgactg ggtttgaggt
      901 ttgtcaggca attcgtgaat tctctagcgt cccgatcatt atgttgacgg ctaagggaga
      961 agatatggat aagattcttg gccttgaata tggggcagat gattatatca caaagccatt
     1021 taatattctg gaagtaaagg caaggatcaa agcaatctta cgtcgaagcg cacatcacga
     1081 tgtgaaagaa gaagaatcag caaaggttgt agaatgcaga ggtttaaaga ttgactgcga
     1141 aagccgcaga gtgcatattc atggcaaaga agtgaacctg acagcgaaag aatttgactt
     1201 gttagagtta ctggtatttc atccaaacaa agtatacagc agagaggatt tgttgaatac
     1261 agtatgggga tatgattatc cgggggatgc aagaacggtt gacgtgcata tccgaagatt
     1321 gagagagaag atcgaagtga atccgggagt accggactat gtacatacaa agtggggggt
     1381 aggttactat tttcagtcct aagaagaaca gatttgcctt tttacatagt atgcagttct
     1441 ttattttctt gtcagttttc cttctgacga cattgatatc agttttattt agtaagttgt
     1501 taaatgatgg atatcgtcag aaaacatatg accagaaagt ggatagtgtg gtcagtcagt
     1561 gtagctggct tggtgatcag gtagttggag tcgattttca tttagatgat ggctccaact
     1621 ctctttctaa tcagattgat gaacttgcag caagtatgaa tgggcggatc atcgtaatta
     1681 agaataatta taagatcatc aaggatactc atacagattt tcagtctaga ttctttttaa
     1741 atgataatgt attggatgtt atggatggaa agaagacaag gattgtatca gagagtggaa
     1801 agtatatcga agtgatggtt ccgatcgtat caaaagctgg aaagcagaat catatcctgg
     1861 gagctgtgca ggcgactgtg ccatctgagg atatgatggg agcagttacg gagatggagc
     1921 atcgcaggaa tatgctgctg gtagcatgta tcatctgtgg attatcaatg gcaatgatca
     1981 tcagttatct gctgacgaga gatttaaaga agattcagag agatgttgat tttattgcgg
     2041 ctggtcatga agatgaatcc attgaagaga caggatttct agaagtacgc agtattgtgc
     2101 agcgttttaa tgagattctt ggaagaatgc agacgttgga agattcaaga caggagtttg
     2161 tatcgaatgt atcgcatgag ttaaagacac caatgacatc gatgaaagtt cttgcagatt
     2221 ccttaattca gcagggagat caggttccgg ctgaattata tcgagaattc atgcaggata
     2281 tcgttgctga gatcgaccgt gagaacgtga tcatttcaga tctgttatct ctggtgaaac
     2341 tagacaagaa agcagcgcag cttcagatca caacagtaag tatcaatgaa ttgttggaga
     2401 ttatcatgaa gcgtttaaaa ccattggctc tgcagcgtaa tatcgaattg atctttgaaa
     2461 gtttccgtcc ggtaacagca gagattgatg aagtgaagtt gtctttaggg cttacgaatc
     2521 tgatcgagaa tggtatcaag tataacaaag atgatgggtg gatccgcgtg acactgaatg
     2581 cagatcataa gtatttttat gtgaaagttg cagattctgg tgttggaatt cctgaggatt
     2641 gccaggatca ggtatttgat cgtttttatc gcgtggataa agcaagatca agagatactg
     2701 gaggcacagg acttggatta gcgatcacaa agagcgttgt ttctcttcat aatggagaga
     2761 ttaagttata cagtaaggtt ggtgaaggaa caacatttac acttagaatt ccattaaagt
     2821 ataaaggcta ggaggaagag atgaagaaaa gaaaattatg catgatcttt ttgctttgtc
     2881 tggctttgat gactttagtg gcaggatgcc ggtcaaaaaa agaaaccaat gaccaaggca
     2941 atcaaaaagg aacagcaatt tactatacaa ataatgatgt tacgaaattg atcatgaaga
     3001 aagaaaatgt ggagcttgag gggaatcagc agcagaaagt gaagatttta ttaaagaaac
     3061 ttcaacagac tccaaagagt gataagatac gctcagtgat tccaaagaga attatgatca
     3121 atggtgtatc agtgaataca aatatcgttg agattgattt ttcgacaggg tataaacgaa
     3181 tttcagagaa tagagatttg atctgccgtg ctggtattgt atatacattg actcagttaa
     3241 aagatatcaa ttatgtatca ttttcaattt ctggagagcc aatgcttgat acagatggta
     3301 cagcaattgg tgctcttgga agagatagtt ttgtatttgg taagttgcca atgaaataat
     3361 ttatgaaaca aagaccgatg gttcttatat ttgccggagt gttatccgga acagccgttg
     3421 tatggaatat tatgtccatg acagcggctt tttatctatt gacaggtatt tgtctgttgg
     3481 cgtccttgat ttttagtgat aggcgttatg gatgggttgt agttgggata cttgcaggaa
     3541 ttttattatc gttaggtttt aagacttcca tgaaacttga tctgatccag gtaaaggaaa
     3601 aggcaacaga atatacaatg ggaagagttt tagaagtatc aaaaacaaag aagggaaagt
     3661 tagcagtact tttaaaaaat aaaaatttgg aggggaagtt ccttttatat acacccgagg
     3721 atgaaaagat cattccagga gatattcttc ggtttcaagg agaattagaa gaatgggaag
     3781 atgcagctaa ccctggccaa ttttcaagca gacattatta tttcagtaaa ggaatttact
     3841 atcatgtata ttcgaaaaat attgagatcg aaggacatca aaatatatat tttcaggaaa
     3901 agatcttgca atgtagagag tacatgaaac atcagttaga aattcaatac aaaaatgaag
     3961 taagtgattt cttaaagggg atgttaatag gtgataaaaa tggactgagt gatgaggtta
     4021 aagatgactt taaggaaagt ggattaattc atttacttgc ggtatcagga cttcatatat
     4081 cgttggcagg aagaagggta tataaattgg ttcgcaagtg gtgtggaaat tttgtagttg
     4141 gttcactggc aggaatgaca ggcgcagtgt tttattgtat tttaacaggt ggatcgggat
     4201 cgtcacttcg tgcagtgatg atgttgggtg tttacttcct gtcagagata ttaggagaac
     4261 actatgatat gatgtctgca ggagcttttg caggaattgt cttacttata atgtgtccgt
     4321 acagaatcta tgatacgggt tttttatatt catttacagc agtttttgtg atcgcaatct
     4381 atcaacttgt taaaccaaag atgaaaggta aatattataa gttgaaggaa tctttatcgt
     4441 tttgtatttt tatccagata gggatgctgc cattgatcat atattttcaa tatgaagctc
     4501 cagctttttc ttttcttgcg aatgtagcag cagtaccact ggcaacgtgt gcttttactt
     4561 tagcttttct tttgattttt ttgccgtata ccgtatttca tgaagcaatc tcatggatga
     4621 tccaaggggt tttgtggatt tccagacagt cttatgggat gttgacgatc ggacatgtac
     4681 catttttatg ggtactctta ttttatttta tgaccggatt gtggatatgg aagaaaaatg
     4741 gacaaaatcg acatatcagg atcagtctgg cttatgttat catgatcatt tt
//
LOCUS       DAOMUE010000147         4791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4959_length_4791_cov_3.375845, whole genome shotgun sequence.
ACCESSION   DAOMUE010000147 DAOMUE010000000
VERSION     DAOMUE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4959_length_4791_cov_3.375845"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="dnaE"
                     /locus_tag="PLC71_07200"
     CDS             complement(<1..1055)
                     /gene="dnaE"
                     /locus_tag="PLC71_07200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRM02464.1"
                     /translation="MSFTHLHVHTEYSLLDGSSKIKEPVHQAKELGMDSIAITDHGAM
                     YGVIDFYRAAKAEGIKPIIGCEIYVTTGSRFDKEASQGDKRYYHLVLLAQNDTGYHNL
                     MKIVSRGFTEGFYYKPRVDYEVLEEYKEGLIALSACLAGEVATYIRENNYEKAKNTAL
                     RLQDLFGKDNFFLELQDHGIPDQTKVNTALLKMSKETGIDLVATNDIHYTFKEDAEAH
                     DILLCIQTGKKVQDEDRMRYEGGQYYLKSPEEMQRLFPYAREAIENTGKIAKRCNVEI
                     VFGEQKVPEYDVPEGYTAVTYLNHLCEEGLKRRYPNITKELRERLDYELKTIENMGYV
                     DYFLIVWDFIHYAKEHG"
     gene            1217..2575
                     /gene="murF"
                     /locus_tag="PLC71_07205"
     CDS             1217..2575
                     /gene="murF"
                     /locus_tag="PLC71_07205"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRM02465.1"
                     /translation="MENITIQDILEATDGTLLCGDPSMKIDHLSTNSMEIGPNTLFVP
                     IIGERVDAHIFIPNALKEGGACLTQEHTEASGNAPYIKVPDTLTAMQQIAAYYRNKMS
                     LPIIGVTGSVGKTTTREMIAHVLKGKYKVFETIGNQNSQVGVPLTLDHLTSEDEIGVL
                     EMGMSEKGQITTLGNIIHPNVGVVTNVGVSHIEMMGSRDNICIEKLDIQNGLPEDGVL
                     FLNGDNDMIRKHIDYVKKPYEFYGFADDCTYRAEKIREKGGQTLFEFHYGDMKEPITL
                     NVLGKHNVSNALAAIAIGLRYDVPMSAIKAQLSTFSGQRQNIIHVNDYILIDDAYNAS
                     PDSMKASLSILSEFKTRGRKIAVLSDMLELGPDSPEYHKEVGRFIATTKVTDLFITGE
                     LSRHYIEEAWKENPSLHTRAFSSNGELIAFLETYLKKNDVVLIKGSNGMNLKEVSKAL
                     MA"
     misc_binding    2649..2890
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3001..4704
                     /locus_tag="PLC71_07210"
     CDS             3001..4704
                     /locus_tag="PLC71_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HRM02466.1"
                     /translation="MNSLTIVAIAIVVLVAGYALYGRWLAKTWGIDPKAKTPAYTHED
                     GEDYIPTPKAVVFSHQFSSIAGAGPVTGPIIAAMFGWLPVLLWLLIGGIFFGAVQDFT
                     ALYASVKNEGKSMGVLIEKYIGKTGRKLFLLFSWLFTLLVIAAFTDMVAGTFNGFTVT
                     GAKSSPNAAAASISMLFILGAVVFGLFTKYVKPNQKVEFVAGLVLLLVMLAVGIAFPL
                     YFTKNTWIAVVMVYLFLASVMPMWLMMQPRDYLSTFLLVGMIIGAVLGVFVAHPAMNL
                     PAFSGFNVGGKSLFPTLFITIACGAVSGFHSLVSSGTSSKTVSNEKDMLCVGYGSMMV
                     ESLLGVIALVVVGAAAVGGKMPEGTPFQIFSGNVAGFLTLLGIPKHVATCFMTMCVSA
                     LALTSLDSVARIGRMSFQELFMGEAADGSDLTGVRKLFTNKYFSTVITLFFGFLLCLG
                     GYNNIWPLFGAANQLLASLVLIALSVFLLTTGRKGWMLYAPMCIMLVVTFTALVQAVI
                     GIFTKIFVTGGFVFMIDGLQLIVALLLMALGLMVAVSCFKKLFQKSHEGADNRSTHAT
                     V"
BASE COUNT         1387 a          945 c          920 g         1539 t
ORIGIN      
        1 atcccatgtt cttttgcata atgaatgaaa tcccatacga tcagaaaata atcaacatat
       61 cccatatttt caatagtttt caattcataa tccagccgct ctcttaattc ttttgtaata
      121 ttcggataac gacgttttaa gccttcttcg cataaatggt tcagatacgt aactgcagtg
      181 tatccttctg gcacatcgta ttctggcact ttctgttctc caaatacgat ttcaacatta
      241 caacgtttgg caatcttgcc tgtattctcg atcgcttctc ttgcatatgg gaacaatctc
      301 tgcatttctt ccggagattt taaataatac tgacctcctt cataacgcat acggtcttca
      361 tcctgtactt tcttccccgt ctggatacaa agcaggatat catgtgcctc ggcatcttct
      421 ttaaatgtat aatgaatatc atttgtagca accagatcaa ttcctgtctc ttttgacatc
      481 ttaagcagtg ccgtgtttac ttttgtctga tctggaattc cgtgatcttg caattccagg
      541 aagaaattgt cttttccaaa aagatcctgt aatctcaacg ccgtattctt tgctttttcg
      601 tagttatttt cacggatata agttgcaact tccccagcaa gacatgcact taatgcgatc
      661 agaccttcct tgtattcttc cagaacttca taatctactc gtggcttgta ataaaatcct
      721 tcggtaaacc ccctggaaac gatcttcatc aagttatggt aacccgtgtc attttgggca
      781 agaagtacca ggtgatagta tcttttatct ccctgactgg cttctttatc gaatctcgaa
      841 ccagttgtta cataaatctc gcatccaatg atcggtttga tcccttctgc tttcgctgcc
      901 ctgtaaaaat cgatcactcc gtacatcgct ccatgatcgg tgatcgcaat gctgtccatg
      961 ccaagctctt ttgcctgatg aactggctcc ttgatcttac tggaaccatc cagcagactg
     1021 tattctgtat ggacatgtaa atgtgtaaaa ctcatgaacc ctcttttcta atatgttttt
     1081 tgtattttct ggctatcatt ttatcataca acttcatttt atgctataat ctgtagataa
     1141 cagcataaat tttttaaacc gttatcatca atcacaccta tgctgaaatc taacagtgaa
     1201 agaaacgagg ttttctatgg agaatataac aattcaagac attttagaag caacagacgg
     1261 aacactttta tgtggtgatc cttccatgaa gattgaccac ttaagtacga attctatgga
     1321 gatcggtcct aacactttat ttgtaccgat catcggagaa cgtgttgatg cccatatttt
     1381 tattcctaat gcattaaaag aaggcggtgc ctgcttaaca caagaacata cagaagcttc
     1441 aggaaatgct ccatacatca aagttcctga tactttgaca gccatgcagc agattgctgc
     1501 ctattacaga aataaaatgt ctcttccgat catcggcgtt actggaagtg ttggtaaaac
     1561 aacaacccgc gagatgatcg cacatgtttt aaagggcaaa tacaaagtat ttgaaacgat
     1621 tggcaatcaa aacagtcagg ttggtgttcc attaacatta gatcatctga catccgaaga
     1681 tgagatcggt gttcttgaga tgggaatgag tgaaaaggga cagatcacaa ctcttggtaa
     1741 tatcatccat ccaaacgttg gtgttgtcac aaatgttggt gtttcacaca ttgagatgat
     1801 gggttcccga gacaatatct gtatcgaaaa attagatatc cagaatggtc tgcctgaaga
     1861 tggtgtccta ttcttaaatg gagataatga tatgatccgt aaacatatcg attatgtcaa
     1921 aaaaccatat gaattttacg gtttcgcaga tgattgtact taccgtgcag aaaaaatccg
     1981 tgaaaaaggt ggacagactt tatttgaatt ccactatggt gatatgaaag aacctatcac
     2041 tttaaatgta cttggaaagc ataatgtatc caatgcatta gccgccattg cgatcggact
     2101 ccgctatgat gttccaatga gtgcaatcaa ggcacagtta tccacatttt ctggtcagcg
     2161 acagaatatt atccacgtta acgactatat tctgatcgat gatgcgtata atgcaagccc
     2221 cgattccatg aaagcaagtt taagtatttt atctgaattt aagacacgtg gaagaaagat
     2281 cgctgtctta tctgacatgt tagaacttgg accagattca ccggaatacc acaaagaagt
     2341 gggacgtttt atcgctacaa caaaagtgac agatctcttt atcactggtg aactttccag
     2401 acattatatt gaggaagcat ggaaggaaaa tccttctctt catacccgtg cattttcttc
     2461 taacggagaa ctgatcgctt tccttgaaac ttacttaaag aagaacgatg tcgtactgat
     2521 caaaggctct aacggtatga atcttaagga agtaagcaaa gccttaatgg cttgattgta
     2581 tcaaaaatag aacattattt cctcttgaca ttcttttttt acaggactat tatgaatgca
     2641 acgaaataca gaatgcaatg agcaggacac attgatattt gaattctttt tcagagagtt
     2701 gccggttgct gtgaggcaat aaagaactta tgtccatatc ccctgcgagc agtcaactga
     2761 acatgatgat tcattagtaa gtgcgaccgg aatccttccg ttatcaagga gcacattaaa
     2821 tgatgtgact aagggattac gatttgagta ataaaaagaa gtggtaccgc ggagaatatt
     2881 cgtcttctga aatgctgagc aggcatttca ggagattttt tttactcatt tttattgtat
     2941 cctgtgtttt gaatacattt tattttatga tagagtttat tttaagagga gtgattaaga
     3001 atgaatagct taacaatcgt agcaatcgca atcgtcgtat tagttgctgg ttatgccctt
     3061 tacggacgct ggttagctaa gacatgggga atcgatccta aagcgaaaac cccagcttac
     3121 acacacgaag atggtgaaga ttacatcccg acaccaaaag ccgttgtatt ttcccatcag
     3181 ttttcatcca tcgcaggtgc aggtcctgtt accggaccga tcatcgccgc aatgtttgga
     3241 tggctgccag ttcttctctg gctgttgatc ggtggtatct tcttcggagc cgttcaggat
     3301 tttacagctc tttacgcttc tgtaaagaac gaaggaaaat ccatgggtgt cctgatcgag
     3361 aaatacattg gtaaaacagg aagaaaatta tttcttctgt tctcctggtt atttacttta
     3421 cttgtaatcg cagctttcac agatatggtt gccggaacct ttaacggatt tacagtaact
     3481 ggtgctaaaa gttcaccaaa cgctgccgct gcttctatct ctatgttatt tatcttagga
     3541 gctgttgttt tcggattatt cacaaaatat gtaaaaccaa accagaaagt agaatttgtt
     3601 gcaggacttg tattactgct cgttatgtta gccgttggta ttgccttccc attatatttt
     3661 acaaaaaata cttggatcgc agtcgttatg gtttacttat tccttgcatc tgttatgcca
     3721 atgtggctaa tgatgcagcc tcgtgactat ttaagtacct tcttattagt cggaatgatc
     3781 atcggtgctg ttcttggtgt atttgttgcc catccagcaa tgaacttacc agcattcagc
     3841 ggatttaatg ttggtggaaa aagcttattc cctacattat ttatcacgat cgcctgtggt
     3901 gctgtttcag gattccacag tttggtatct tccggaacat catctaaaac agtcagcaat
     3961 gaaaaagaca tgctttgtgt tggatatgga tctatgatgg tagaatcttt attaggtgtt
     4021 atcgccttag ttgttgtcgg tgcagcagct gtcggtggta aaatgcctga aggaacacca
     4081 ttccagatct tctcaggaaa tgtagctgga ttcttaacac tacttggaat ccctaaacat
     4141 gtagctactt gctttatgac aatgtgtgtt tctgcacttg cattaacatc ccttgactct
     4201 gtagcaagaa tcggacgtat gtcattccag gaattattca tgggagaagc tgctgatggc
     4261 tctgatctta caggggtaag aaaattattt acaaacaaat atttctcaac tgttatcaca
     4321 ttattcttcg gattcttatt atgcttaggt ggatacaaca acatctggcc attattcgga
     4381 gcagcaaacc agttattagc atctttggta ttgatcgcat tatctgtctt cttattaaca
     4441 acaggaagaa aaggatggat gttatacgca cctatgtgca tcatgttagt tgtaacattt
     4501 acagcccttg tacaggccgt gatcggaatc tttacaaaga tctttgtaac aggtggattc
     4561 gtatttatga tcgatggatt acagttgatc gtcgctttac ttctgatggc acttggactt
     4621 atggttgcag tttcctgctt taagaaactg ttccagaaaa gccatgaagg agcagataat
     4681 agatctacac atgcaactgt gtaaatttta attgtatgac ggaaatgtat taagtattca
     4741 taaaaatata aagaaagata gaggtacgaa aaacgaattt catttcgtac c
//
LOCUS       DAOMUE010000148         4779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4970_length_4779_cov_2.502117, whole genome shotgun sequence.
ACCESSION   DAOMUE010000148 DAOMUE010000000
VERSION     DAOMUE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4970_length_4779_cov_2.502117"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PLC71_07215"
     CDS             <1..802
                     /locus_tag="PLC71_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRM02467.1"
                     /translation="SFYFKYERKSRKKKITYKTSKKSVATVNKKGVIKGKKYGTAKIT
                     MKASGVKTKKITVYVRKAATGIKLSSAQTINFYKTGNTAQIKATALPGGKQMASKTLT
                     YKSSNPQVAVVNSKGKVTAKKGGYSRIQISTTARSGKICTKDVDVFVYDGFDDVCSET
                     SGSKTTFTFNPNWKSVTIYFTSQTGKQYSYKVDSIKTEFNMLANVKGFADERNGVAVS
                     KDSARKANIINFKIIETGEDYDVLADAQRYQLTFYKALNNKVTFNVEK"
     gene            1047..1544
                     /gene="infC"
                     /locus_tag="PLC71_07220"
     CDS             1047..1544
                     /gene="infC"
                     /locus_tag="PLC71_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRM02468.1"
                     /translation="MINGQIRDKEVRLIANDGEQLGVMSAREAQKLADEAGLDLVKIS
                     PKAKPPVCKIIDYGKYKYEQTRKEKLAKKKQKVIDVKEVRMSPNIDTNDLNTKINHAK
                     KFLAKGARVKVTLRFRGRELAHVNASKYILDDFAKALEDVATIDKKPKFEGRSMTMFL
                     APKNN"
     gene            1601..1798
                     /gene="rpmI"
                     /locus_tag="PLC71_07225"
     CDS             1601..1798
                     /gene="rpmI"
                     /locus_tag="PLC71_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRM02469.1"
                     /translation="MPKMKTCRAAAKRFKKTGTGKLKRNKAYKSHILTKKSPKRKRNL
                     RKAAMTDATNEKNMKKILPYL"
     gene            1828..2184
                     /gene="rplT"
                     /locus_tag="PLC71_07230"
     CDS             1828..2184
                     /gene="rplT"
                     /locus_tag="PLC71_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRM02470.1"
                     /translation="MARIKGGMNAKKKHNRVLKLAKGYRGARSKQYRVAKQSVMRALT
                     SSYAGRKQRKRQFRQLWIARINAATRMNGLSYSKFMHGLKLAGVDINRKMLSEMAISE
                     PEAFKALVDVAKEKLA"
     gene            complement(2194..3516)
                     /locus_tag="PLC71_07235"
     CDS             complement(2194..3516)
                     /locus_tag="PLC71_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HRM02471.1"
                     /translation="MTFYDIFTYLCTFTIIIPLAVFCLFPVIDHLKTPIRHLLIKISL
                     VFVCYFVLLIIPYMIFQQDLGNILVFLAVPVFFYLFQKETNLMLPASLFVMLTACCLG
                     SLSYVIYHTFGVIFHPHGQSTEFYPESMIAQLIFLVFADIILYKPARKYLGWMVTNFH
                     NAFVWRIACIFPCCFTFLVFTYIPHNYYDIYTYHDLHVYFSMMLTLLVFVFLLYVLFY
                     TIIHSYVENQYILEEQKILEIQAKEYSQLSQHVQETREIRHDFRHQITVISGLLNQGN
                     YEELKEYLSQYESSISLQTKIYCRQPAVNAILSHYDLLCAQDKIKTKFAVDFPTLSPI
                     SSVDFCIVLGNLLENAYLECKTLQKYEKFIHLKARQTSPGAFVLLIENPYEHEIKKTD
                     SGFFLSSRRKNCVGTGLKSVTAICKKYDGHLSIETDNHRFKVKMFLQC"
     gene            complement(3523..4239)
                     /locus_tag="PLC71_07240"
     CDS             complement(3523..4239)
                     /locus_tag="PLC71_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRM02472.1"
                     /translation="MKLAIVDDNKLEQELIFNTLRTYEHERNISLDISSYSDGNSFLN
                     TFVPGDFDLIFMDIYLNELNGIDIVRKIRQMDSKVMIVFLTTSKEYIFEAAPFHFFDY
                     ILKPFESTQIFHVLDDALALLPEQEAELSFEYRSFDVHFPLSKIQYIYSINHEVIIHT
                     TNGTHAFRLPFYSVTDHLDDSRFLQCNRGIMLNMDYIKTMESDYFEMTDSKCFPIKTK
                     GRKQIREQYIKYQFLKLEEA"
     gene            4422..>4779
                     /locus_tag="PLC71_07245"
     CDS             4422..>4779
                     /locus_tag="PLC71_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRM02473.1"
                     /translation="MNKIYDKVKECYKQVNQMTEFKPQIALVLGSGLGDYAEKIKIET
                     VIDYHEIKGFPVSTVQGHKGRFVLGYVEDIPVMIMEGRVHYYEGYSMSDVVLPIRLMK
                     MMGAEILFLTNASGGIQ"
BASE COUNT         1836 a          725 c          984 g         1234 t
ORIGIN      
        1 ctccttttat tttaaatatg aaagaaaatc tagaaagaaa aaaatcacat ataagacatc
       61 caaaaaatct gtagcgacag taaataaaaa gggtgtgatc aaaggaaaga aatacggaac
      121 tgccaagatc acaatgaaag cttctggagt gaaaacaaag aagatcactg tatatgtaag
      181 aaaagcagcg acagggatca aactttcatc tgcacagacg atcaatttct acaagacagg
      241 aaacactgcc cagatcaaag caacagcttt acctggagga aaacagatgg catcaaaaac
      301 tctgacttat aagagcagta atccacaggt agcagtggtt aacagcaaag gaaaagtgac
      361 agctaaaaaa ggcggataca gcagaattca gatttctacc actgcaagat caggaaagat
      421 ctgcacaaaa gatgttgatg tttttgtata tgatggattt gatgatgtat gcagtgaaac
      481 atctggaagc aagacaacat ttacatttaa tccaaactgg aaatctgtca caatctattt
      541 tacaagtcaa acaggaaaac aatattctta taaagtggat agcatcaaaa cagaatttaa
      601 tatgctggcg aatgtcaaag gatttgcaga cgaacgtaat ggtgtagcag tgagcaaaga
      661 cagtgcgaga aaggcgaaca tcattaattt caagatcatt gaaactggcg aagattatga
      721 tgtattagcg gatgcccaga gatatcagct gacattttat aaagcgttga ataacaaagt
      781 gacattcaat gtagaaaaat aaaaagataa gaaaaatact tgacaataaa aatgaaagca
      841 tgatataata tcaaaagttg tgaagaacaa cagaaacaac aaagaagagt atccactctc
      901 accttagcac atatgataga ttaggaagtg acttgggtta atatttaaga atatgatatt
      961 tcgtgatcat gcttgaattt tatgtggata gtcttataac tatccacttt tttaattatc
     1021 atggaggtgt aattattagc gatttaatga ttaacggaca gatcagagat aaagaagtac
     1081 gtctgatcgc gaatgatggc gagcagcttg gagttatgtc cgcaagggaa gcacagaagt
     1141 tagcagatga agcaggatta gatcttgtca agatctcacc aaaggcaaaa ccaccggttt
     1201 gtaagattat cgattacggt aagtacaaat acgagcagac aagaaaagaa aagctggcaa
     1261 agaagaaaca gaaagtcatc gatgtgaaag aagtccgcat gtcaccaaac attgacacaa
     1321 acgacttaaa cacaaagatt aatcatgcaa agaaattcct tgcaaaaggt gcgagagtga
     1381 aagtcacact tcgattcaga ggaagagaac ttgctcatgt gaatgcaagc aagtatatct
     1441 tagatgattt tgcgaaagca ttagaagatg tagccaccat cgataagaaa ccaaagtttg
     1501 aaggaagaag catgacaatg tttttagctc caaagaacaa ctaggagttt aagatattca
     1561 aatatgagta gatacaagca ggattaagga ggaaataacc atgccaaaaa tgaagacatg
     1621 tagagcagct gcaaaaagat ttaaaaagac aggaacagga aaattaaaaa gaaataaggc
     1681 atacaaaagc catatcttaa caaagaaatc tcctaagaga aagagaaatt taagaaaagc
     1741 tgcaatgaca gatgcaacaa atgaaaagaa catgaagaag attttaccat atctgtaaga
     1801 tggatcgtta aggaggaaat gtaagcaatg gcaagaatca aaggcggaat gaacgcaaag
     1861 aaaaaacaca atagagtatt aaaactggct aaaggatata gaggagctag atctaaacag
     1921 tatagagtag caaaacagtc tgttatgaga gctttaacaa gttcttacgc aggaagaaaa
     1981 cagagaaaac gtcagttcag acagttatgg atcgcacgta tcaacgctgc tacaagaatg
     2041 aacggacttt cttacagcaa attcatgcac ggattaaaat tagctggtgt tgacatcaac
     2101 agaaaaatgt tatctgagat ggcgatcagc gaaccagaag cattcaaagc tttagttgac
     2161 gtagcaaaag aaaaattagc ttaatcaata gaattaacat tgtaaaaaca tcttgacttt
     2221 aaagcggtgg ttatcagtct caatagacag atggccatcg tattttttac atatggcagt
     2281 gactgattta agacctgtac cgacgcagtt tttgcggcga gaggataaga agaagccact
     2341 gtctgttttt ttgatctcat gctcataagg gttctcgatg agcagaacaa aagcaccagg
     2401 agatgtctgt ctggctttga gatggatgaa tttctcatac ttctgtaatg tcttacattc
     2461 aaggtaagca ttttctaaaa gatttccaag aacaatacaa aaatcaacgg aagagattgg
     2521 agagagcgtt ggaaaatcaa cggcaaattt tgttttgatc ttatcttgtg cacataaaag
     2581 atcataatgc gataaaatgg catttacagc gggctgacgg cagtagatct tagtctgtaa
     2641 agaaatagat gattcatatt gggacagata ctcctttaat tcttcataat ttccttgatt
     2701 taacaatcca gagataacag tgatctgatg acgaaaatca tggcggattt ctcttgtctc
     2761 ctgtacatgt tgggaaagct gggagtattc ttttgcctga atctccagaa tcttttgttc
     2821 ttccaatatg tactgatttt ctacataact gtggatgata gtataaaata agacatataa
     2881 aagaaataca aagaccagta aagtgagcat catggaaaag taaacatgta gatcatgata
     2941 ggtataaata tcataataat tgtgtggaat ataagtaaaa acgagaaaag taaaacagca
     3001 tgggaaaata cacgcaatcc gccagacaaa ggcattgtgg aaattcgtaa ccatccagcc
     3061 gagatatttt ctggcaggtt tatataagat gatatcggcg aaaaccagaa agatcagttg
     3121 tgcgatcatg gattctggat aaaattctgt actttgtcca tgtggatgaa agatgacacc
     3181 gaatgtatga tagataacat aagaaagaga accaagacaa catgcagtca gcataacaaa
     3241 gagagatgca ggaagcatca ggttcgtttc tttttgaaat agataaaaga acacaggcac
     3301 agcaaggaat acaagaatat tgcccagatc ctgttgaaag atcatatacg gaatgatcaa
     3361 cagaacaaaa tagcatacaa aaacaaggct gatcttgatc agcagatggc ggatcggagt
     3421 ctttagatga tcgattactg gaaacaggca aaaaactgcc agtggaatga tgattgtaaa
     3481 tgtacataaa tatgtaaaaa tatcataaaa agtcataaaa gatcatgcct cctcaagttt
     3541 caaaaactgg tatttgatat attgctctcg gatttgcttg cgtcctttcg ttttgatcgg
     3601 aaaacacttt gaatctgtca tttcgaagta atcggattcc atcgtcttaa tatagtccat
     3661 attcaacatg attccacggt tgcactgaag aaatcttgaa tcatccagat gatctgtgac
     3721 agaataaaaa ggaagccgga aagcatgtgt tccattcgtt gtatgaatga tcacctcatg
     3781 attaattgag taaatatatt gaatctttga taatggaaaa tgaacatcaa aagaacgata
     3841 ttcaaaagat aattcagctt cctgctcagg aagaagcgct aaagcatcat caagtacatg
     3901 aaagatctgt gtggattcaa aaggctttaa aatataatca aaaaaatgaa aaggggcagc
     3961 ttcaaaaatg tattctttac ttgttgttaa gaatacgatc atcactttgg aatccatctg
     4021 acggatcttg cgtacaatat cgatcccgtt cagttcattt agataaatat ccataaagat
     4081 cagatcaaaa tctccaggaa caaaggtatt caaaaaagaa ttaccatcac tataagaaga
     4141 aatatcaagg gagatgtttc tttcatgttc ataagtacga agagtgttga aaatgagctc
     4201 ctgttctaat ttattatcat caacgattgc taacttcata ttagtactcc ttgttgtagg
     4261 gaaattgggg tttctttaag tataacttgc aaatgaatgg aaagcaagaa atgggaacta
     4321 taatttttgt aaacaatagg atgtgtgtgg aatgcatttc attttgtaca aaaatgtgtc
     4381 ataatgtaag aaaaagaact ggcaggaagg agatcgataa tatgaataaa atatatgata
     4441 aagtaaaaga atgttataaa caagtaaatc aaatgacaga gtttaaacca cagatcgcat
     4501 tggtcttagg ttccggtctt ggagattatg cggagaagat caagattgag actgtgatcg
     4561 attaccatga gatcaaagga tttccagttt ctacagtaca gggacataaa ggaagatttg
     4621 tacttggtta cgtagaagat attccagtaa tgatcatgga aggacgagtt cactattatg
     4681 aaggatattc catgagtgat gttgtactgc cgattcgact gatgaagatg atgggagcag
     4741 agatcttgtt tttgacaaat gcatccggtg ggattcagc
//
LOCUS       DAOMUE010000149         4774 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_4979_length_4774_cov_2.685527, whole genome shotgun sequence.
ACCESSION   DAOMUE010000149 DAOMUE010000000
VERSION     DAOMUE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4979_length_4774_cov_2.685527"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1828
                     /locus_tag="PLC71_07250"
     CDS             <1..1828
                     /locus_tag="PLC71_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM02474.1"
                     /translation="FLLENNLGEIQRKPRVGIWLEANENQKEEIQKILSDENLQVGNY
                     DPRDRVNETLKVFFNLRPWQTMTTQKLAEKLFLSVPTMLKVLKECEEWLEVYHISLVN
                     ERGKGYRLKSEENEYRVALKNLIMMKKPGEEIKENIDYFFSNIDVSAIRKCIIQTENE
                     WDYHFTDESFYEILIYCCIAYKRKELALPLVSDYFPEELKILKKYNEYAFTVAIFEKL
                     EEVFHVHFLIEDVLFLSIQILCSKFIGISDVDVTLSQVKKYDNKLVDFVDRMLKVIRD
                     ILDVDLTSDEKVKESLIIHLRPTIFRLRYGTPQKNALIDFIKKEYKNVFRASWAISIL
                     FEEYYGLQITEDEIGYIVLYIQAAIERKKHQYRTVMVSNFNMGHTQLVKEKIKKSVPE
                     IDEIKFVSIHDFKIADYEDYDIIISTEERKEKDKRIVVIPNILNETGISTLRAHLDNM
                     NSIMIENATPFSPECFILFSPEFIFTDLEVKTKEECLKIMSQAMEEKHVVTEDFYDSV
                     MDRESKTTTTIGNGVSLPHGSPTAVNESKVGIAILKNPIMWDNEQVQVVFLLAFRLST
                     RDEISRIQMFYKEYVTLIDSEEKLEKLKSMKSNIELYKYLIQ"
     gene            1860..2309
                     /locus_tag="PLC71_07255"
     CDS             1860..2309
                     /locus_tag="PLC71_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM02475.1"
                     /translation="MDIKSVLDERVIDLNMTAKNKKEAIEHLAGKLKDAGYIADVDSY
                     IEDIYLRESQGQTGIGHYVAIPHGKSDSVTQVGIAIGKVQDEIEWETLDGNGVKLIFL
                     FAVGNDNENAKTHLRLLAEVARTLGNDDAVEALLNASTVEDLKAVFA"
     gene            2621..2932
                     /locus_tag="PLC71_07260"
     CDS             2621..2932
                     /locus_tag="PLC71_07260"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HRM02476.1"
                     /translation="MKIVSVCACTSGIAHTYIAKEKLIRAAESMGHTIKVETQGTIGT
                     EDELTAEEIKEADVVILAVDIAITGTERFEGKPTIKVPTNVCVKAPKKLIEKIENELG
                     K"
     gene            2972..4060
                     /locus_tag="PLC71_07265"
     CDS             2972..4060
                     /locus_tag="PLC71_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HRM02477.1"
                     /translation="MKKLGAELKRHALTAISYMLPLVVASGLLIAIGNLMGGENVTEL
                     SKMTLPSALTTLGVMGMGLLPSFIAGYIAYSIADRPGIAPGFLMGQIASFLGAGFLGG
                     MVGGYLVGYIALFIKNNLKVPKWAEALMPMMIVPTLSAIIAGLIMFFVVGTPITMATK
                     ALTNFITGLDQSSKAVYGFIIGALGCIDFGGPISKVPNLICDGLLLEGITGPEAIKVL
                     AAMVPPLGITFSLVLSKLLKKNIYTAQEKENIKVAFPMGLCMISEGVIPIAMNDIIRT
                     VICTATGCGVTGAISFSLDVGSKVPSGGVFVIPAMSNPLAALIALLAGTAVTGVLLVL
                     IKKKKTEEDEPMVDEKEEDLDLSGFTIS"
     gene            4083..>4774
                     /locus_tag="PLC71_07270"
     CDS             4083..>4774
                     /locus_tag="PLC71_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRM02478.1"
                     /translation="MYVSMKGMLKRANEGNYAVMAINCFNIETARAVINAAQSLRAPI
                     IINIVQEHMVNHCDSNLIAPIVKKLAQRASVEVALNFDHGEEIGLLKKALVDGYSSVM
                     VDASRYNLEGNIRMTREIVEFAKQFDASVEGEIGCMGASEGDNYTDDDMKTNPDEALR
                     FAKETGIDALAISFGSSHGNYPEGYVPEFDFERLKEIKEKTKMPLVLHGGSGSGDENI
                     KRCVEFGINKINV"
BASE COUNT         1797 a          658 c          994 g         1325 t
ORIGIN      
        1 cttcttatta gaaaataatc ttggagaaat tcagcggaaa ccacgagttg gaatttggct
       61 agaagcaaat gaaaatcaaa aagaagaaat acagaaaata ttatcagatg aaaatctgca
      121 agtaggaaat tatgatccaa gagatagagt aaatgagaca ttaaaggtat tttttaatct
      181 gcgtccatgg cagacaatga caacacagaa actagctgag aagttattct taagtgttcc
      241 aacaatgctg aaagtattaa aggaatgcga agaatggctg gaagtatacc acatttctct
      301 tgtgaatgaa agaggaaagg gatatcggct caaaagtgaa gaaaatgaat acagggttgc
      361 attaaagaat ctgatcatga tgaagaaacc aggagaagag atcaaagaga atatagatta
      421 tttcttttct aacattgatg tttctgcgat cagaaaatgt atcattcaaa cagaaaacga
      481 atgggattac catttcacag atgaatcatt ttatgagatc ttaatttatt gttgcattgc
      541 gtataagaga aaagagcttg cattaccatt agtcagcgat tattttccag aggaattaaa
      601 aatccttaag aaatataatg aatatgcatt tacggtagca atctttgaaa aattagaaga
      661 agtatttcat gtacattttt tgatagaaga tgttttgttt ttatcaattc agattttgtg
      721 ttcaaagttt attggaatat cagatgtaga tgtgacatta agtcaggtaa agaaatatga
      781 caataaattg gttgattttg tagatcgcat gttaaaggta atcagagata ttttagatgt
      841 tgatctcact tcagatgaga aagtaaaaga aagcctgatc attcatttaa gaccaacaat
      901 tttcagactt cgttatggaa cgccacaaaa aaatgcattg attgatttta taaaaaaaga
      961 atataagaat gtatttcgtg caagctgggc gatcagtatt ttgtttgaag aatattatgg
     1021 acttcagatt acagaagatg aaattggata tatcgtgtta tatattcagg cagcgatcga
     1081 aagaaaaaaa catcagtacc gcacggtaat ggtttctaat tttaatatgg gtcatacaca
     1141 attagtaaaa gaaaagatta agaaatctgt gccagagatt gatgaaatta aatttgtaag
     1201 tattcatgac tttaagattg cggattatga agattatgac attatcattt cgacagaaga
     1261 gagaaaagaa aaagataaga gaattgtagt cattccaaat atcttaaatg aaacaggaat
     1321 ttctactttg agggcacatt tagacaatat gaattctata atgatagaaa atgcaacacc
     1381 attttcacca gaatgtttta ttttattttc accggaattt atatttactg atctggaagt
     1441 caaaacaaaa gaagaatgtt taaaaataat gagccaggca atggaagaaa agcatgttgt
     1501 tacagaagat ttttatgatt cagttatgga tcgtgagagt aaaacaacaa caacgattgg
     1561 aaatggggtc agtcttccgc atggttcacc gacagcggtt aatgaatcaa aagttgggat
     1621 agcaatctta aaaaatccga tcatgtggga caatgaacag gttcaggtag tatttttact
     1681 ggcgttccgt ttaagtacca gagatgagat cagcaggatt cagatgtttt ataaagaata
     1741 tgtaacattg atcgattcag aggaaaaatt agagaaatta aagagtatga aatcaaacat
     1801 tgagttatat aagtatctga ttcaataagt aaaaagaaaa gttaaaagga gaattaaaaa
     1861 tggatattaa aagtgtatta gatgaaagag ttatcgactt aaatatgaca gcaaagaata
     1921 agaaagaagc gatcgagcat cttgcaggaa aattaaaaga tgccggatat attgcggatg
     1981 ttgattctta tatagaagat atttatttga gagaatcaca gggacagaca ggaattggac
     2041 actatgtggc aattcctcat gggaaaagtg attctgtaac acaggtagga attgcgattg
     2101 gtaaagttca ggatgagatc gaatgggaga cactggatgg aaatggtgta aaactgatct
     2161 ttttattcgc agttggaaat gataatgaga atgcaaaaac tcatttaaga ttattggctg
     2221 aagtagcaag aaccttaggt aatgacgatg cagtagaagc tctgttgaat gctagcacag
     2281 tagaagatct aaaagctgta tttgcataaa taagtttcct aacactctat ataattctta
     2341 ggatccctgt tccaatagga gcagggatct tttgcacttc tatatgttaa aaataaataa
     2401 aaaattaccg catttaaata cttcccttgt ggaatgtgaa acaaaaaaac taccctaatt
     2461 aaaatgccca tattgaaaaa atgtcataat taaatggaaa aaatgccaca ttaaaaagcc
     2521 ctctcttaat gaacacagta aggaaagctg gtgtatgatt agaacataaa gaaagaggta
     2581 ataaacaacc aaaagaaaaa taagaaagga gttgtaagtt atgaaaatcg tatctgtatg
     2641 tgcatgtaca tcaggaatcg cgcacactta catcgcaaaa gaaaaattaa tccgtgcagc
     2701 tgaaagcatg ggacacacaa tcaaagtgga aacacaggga acaatcggaa cagaggatga
     2761 attaacagca gaagaaatca aagaagcaga tgttgtaatc cttgcagtcg atattgctat
     2821 tacaggaaca gaaagatttg aaggaaaacc tacgatcaaa gttcctacaa atgtttgtgt
     2881 aaaagcacct aagaaattga tcgaaaagat cgaaaatgaa ttaggtaaat aagagagaaa
     2941 caaaaaacta tacttaaagg agaagagaac aatgaaaaaa cttggagcgg aattaaaacg
     3001 tcacgcattg acggccattt cctatatgtt acctttagta gtagcatcag gattactgat
     3061 tgcgatcggt aacttaatgg gaggagaaaa cgtaactgaa ttatctaaga tgacacttcc
     3121 tagtgcatta acaacacttg gtgttatggg tatgggattg ttaccatcct ttatcgcagg
     3181 atatattgct tactcaattg ctgatagacc tggtattgca cctggtttct taatgggtca
     3241 gatagcttca ttcttaggag ctggattctt aggaggaatg gttggtggtt atttagtagg
     3301 ttacatcgca ttattcatta agaataactt aaaagtacct aaatgggcag aagcattaat
     3361 gccaatgatg atcgtaccaa cattatcagc gatcattgct ggacttatca tgttcttcgt
     3421 agtaggaaca ccaattacaa tggcaacaaa agccttaaca aacttcatta caggacttga
     3481 tcagtcttca aaagcagttt atggatttat catcggagct cttggatgca ttgactttgg
     3541 tggaccaatc agtaaagtac ctaacttaat ctgtgatgga ttactattag aaggaatcac
     3601 aggaccagaa gcgatcaaag tattagcagc tatggttcca ccacttggaa ttacattctc
     3661 attagtatta tcaaaattac tgaaaaagaa catttataca gcacaggaaa aagaaaatat
     3721 taaagttgcg ttcccaatgg gtctttgcat gatctctgaa ggagttattc caatcgctat
     3781 gaatgatatc attcgtacag ttatctgtac agcaacagga tgtggtgtta caggagcgat
     3841 cagtttcagt cttgatgtag gaagtaaagt accatcaggt ggtgtatttg taatcccagc
     3901 aatgtcaaat ccacttgcag cacttatcgc actgttagca ggaacagcag ttacaggtgt
     3961 tttacttgta ctgatcaaga aaaagaaaac agaagaagac gaaccaatgg ttgacgaaaa
     4021 agaagaagat cttgatttat caggattcac tatttcataa aatcagaaag gacagataag
     4081 ctatgtatgt atcaatgaag ggaatgttaa aacgtgcgaa tgaaggcaat tatgcagtca
     4141 tggcaatcaa ctgctttaac attgagacag caagagcggt gatcaatgca gctcagtcat
     4201 taagagcacc gatcatcatc aatatcgtac aggaacatat ggtaaatcat tgtgacagca
     4261 atttgatcgc accgatcgtc aagaaactgg cacaaagagc aagtgttgaa gtagctttaa
     4321 atttcgacca tggggaagaa atcggattgt taaagaaagc attagttgat ggatattcca
     4381 gtgttatggt agatgcttcc agatacaact tggaaggtaa tatccgaatg acaagagaaa
     4441 tcgtggaatt tgcgaaacag ttcgatgcaa gtgtcgaagg agagatcgga tgtatgggtg
     4501 ctagtgaagg cgataattat acagacgatg atatgaaaac taatcctgac gaggcattac
     4561 gatttgcaaa agaaactgga attgatgcat tagcaatttc ttttggaagt tctcatggaa
     4621 actatccaga aggatatgta ccagaatttg attttgagag actaaaagaa atcaaagaaa
     4681 aaacaaagat gccactggta ctgcatgggg gaagcggttc tggtgatgaa aatattaaaa
     4741 gatgtgttga atttggaatc aataagatca atgt
//
LOCUS       DAOMUE010000150         4752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5010_length_4752_cov_2.459442, whole genome shotgun sequence.
ACCESSION   DAOMUE010000150 DAOMUE010000000
VERSION     DAOMUE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5010_length_4752_cov_2.459442"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLC71_07275"
     CDS             complement(<1..324)
                     /locus_tag="PLC71_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM02479.1"
                     /translation="MRENHILIIDDDLMLLKTAEEILSDQYNVSVAKSGIQALNLLNK
                     NIIPDLILLDIDMPQMDGYETLEEIKKISGCELIPIIFLTGFSEMDYEVRGLKAGAVD
                     YIVKPF"
     gene            complement(321..2735)
                     /locus_tag="PLC71_07280"
     CDS             complement(321..2735)
                     /locus_tag="PLC71_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM02480.1"
                     /translation="MGKWFRQILQGEHSLFRLSLPFRVRLFNVLALVGGMISLINGIS
                     SYANNQDSVILGLNLGIAVLSFVLLFYAYKSGRYQFCYVVTIIMIFLMMFPYLFFKSG
                     GYKGGMVSFYIFGILFTVFMLEGKVMFFTAFMEMVVYIATIMIAYQNPQMVVWFSSEK
                     EVVMDLLIGFCASSISVAAVMYLHFRMYNKQQEILEEARIEAQSANKAKSAFLANMSH
                     EIRTPINVMLGMNEMILRESESKEIRQYAKSIERSGGYLISLINNILDISRIESGKME
                     IEEGKYELRQLLDEVMLIAEKQAEQKSLKMNLIFDKTLPAYLIGDVIHIKQILLNLIN
                     NAVKYTKEGQIDIKVSKNEEETKLIFEVKDTGIGIKEENLPILFDAFMRVDSKKNKKI
                     KGTGLGLAIAKQLAEQMDGMIWVESVYGKGSSFFVQLPMKKVSDGKISNVEWKETDER
                     KRRSFVAPQAKILIVDDNPENLMVTRSLLKRTAVFVDTAASGEECVHKVRQNVYDLIL
                     LDYMMPQMDGIDTIRELKKDVQFHIPVIALTADVTKGIEQTFLREGFCAYLSKPVMWS
                     KLEDLLMKYLRDDLVFIREDLKEEQKIKDEEFKQLKGQLKENDIKIEEGLRLLDGDFM
                     QYRKLMEFFTEYQEEYMRQMQQLMTQNEVKVDEITRMMHTLKSNAKAIGAIHLYEIAK
                     EMEDRGKQKDMEYIMSAYDLLKLEWGRVFKASREFIEQTKNVLFDQKKEEEKDKQSKE
                     EIKEKLKIFITRYQAKEAKEQIQYYRKGKISEEERNILKEMEIRIDQLDFDEAEILMK
                     RWEGME"
     gene            complement(2804..3733)
                     /locus_tag="PLC71_07285"
     CDS             complement(2804..3733)
                     /locus_tag="PLC71_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM02481.1"
                     /translation="MKKTVYLTSKQDGLKLHVLLMEPEQSPKGIVQICHGMAEHKERY
                     EPFMQMLCNNGYISVIHDHRGHGKSVKNASDLGYFYDDSGKAIIEDAHQVTTWMKERY
                     GEELPYHLFGHSMGSLVVRCYLKKYDDELDSLIVCGSPSENKAAKAAGFLAKTACKMG
                     AHKKGKFFQKMALGLYGKALGEDESENGWISYNKENVKAYDENPLDGFVFSNNGFLNL
                     FLLMDETYNKKGWQVKHPSLPILFIAGADDPCIGSKKQYAQAMTTLKKRGYNQVRGML
                     FLNRRHEILNEDDVEKVYDAVLHFLEQHSKRDD"
     gene            3839..4618
                     /locus_tag="PLC71_07290"
     CDS             3839..4618
                     /locus_tag="PLC71_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02482.1"
                     /translation="MKIRIKKVTALLLSCMLLFSISGCQKKRTAKEVLESSLKQSSKL
                     KDADFSGEASYKIANSEASSSSNVTIKMNFDTKLQTVDKDQLKMSMTSTLNMLGQTMD
                     MNMYYSDGYYYMNTNGTKQKIKMDIAGLQKQIQSTTGQTSLSAKYYKDLKLSEKDGNT
                     VLKYSINNDGLNQYVKDVTSQMTTVTGGSNSIKISSLTGTKTLNDKDLPVKESIQMVM
                     ESGDNETGSITLKMNLTYHDPGKSVTVTLPDDLNTYQEISN"
BASE COUNT         1372 a         1050 c          666 g         1664 t
ORIGIN      
        1 aaatggcttt acgatataat caactgcccc tgctttcaag ccccttactt cataatccat
       61 ttctgaaaat ccagtcaaaa atatgatcgg gatcagttca catcctgaga tttttttgat
      121 ttcttctaat gtttcgtatc catccatctg tggcatatca atgtctaata agatcaaatc
      181 tggaatgata tttttattta acagatttaa tgcctgtatt ccactctttg cgacactgac
      241 attatattgg tcagataaaa tctcttctgc tgtctttaga agcataagat catcatctat
      301 gatcaaaata tgattctctc tcattccatt ccctcccatc gtttcataag gatttctgct
      361 tcatcaaaat caagctgatc gatccgtatt tccatctctt tcaagatatt tctttcttcc
      421 tcggatatct tgccttttcg ataatactga atctgttctt tcgcttcttt cgcctgatat
      481 ctggtgataa atatttttaa tttttctttg atttcttctt tactttgctt gtctttttct
      541 tcctcttttt tctgatcaaa cagaacattt ttcgtctgtt caataaattc ccgtgatgcc
      601 ttaaatactc gtccccactc caatttaagc agatcatatg cagacatgat gtattccata
      661 tccttttgtt tccctctatc ttccatttct tttgcaatct catacaaatg aatcgcacct
      721 atcgcttttg cattgctctt taaggtgtgc atcatccttg tgatctcatc gactttcact
      781 tcattttgtg tcatcaactg ctgcatctgt ctcatatatt cttcctggta ctccgtaaag
      841 aattccatca atttacgata ctgcataaag tctccatcca gaagtctaag cccctcttca
      901 atctttatat cattttcttt tagctgcccc ttcagctgct taaattcttc atctttgatc
      961 ttttgttctt ctttcagatc ctcacgaata aatacaagat catctctcaa atatttcatc
     1021 agcaggtctt ctaatttgct ccacatcact ggctttgaaa gatacgcaca aaatccttct
     1081 cttaaaaatg tctgttcaat ccctttcgtc acatcagcag ttaaagcgat cactggaata
     1141 tgaaattgta catctttctt taattccctg atcgtatcga ttccatccat ctgcggcatc
     1201 atataatcaa gtaagatcag atcatatacg ttttgtctga ctttgtgcac acattcctct
     1261 ccgctagctg ctgtatccac aaacacagct gtacgcttca gaagtgatct tgtcaccatg
     1321 agattttccg gattatcatc tacaattaaa atctttgcct gcggtgcaac aaatgatctt
     1381 ctctttcgtt catccgtttc cttccattca acatttgaaa tcttcccatc agataccttc
     1441 ttcattggaa gctgcacaaa aaaactgctt cctttgccgt atacactttc tacccagatc
     1501 ataccgtcca tctgttctgc cagctgtttt gcgattgcaa gtcctaatcc agttcctttg
     1561 atcttcttat ttttcttaga gtctactctc ataaatgcgt caaataaaat aggcaaattt
     1621 tcttctttaa tcccgatccc tgtgtctttt acctcgaaaa taagcttcgt ttcttcttca
     1681 tttttactga ctttaatatc aatctgacct tctttagtgt attttactgc attattgatc
     1741 aggttcaaaa gaatctgttt gatatgtatc acatcaccga tcagatacgc tggcaaggtc
     1801 ttatcaaata tcagattcat ttttaatgac ttttgttccg cctgtttctc tgcgatcaac
     1861 atcacttcat caagcagctg cctgagttca tattttcctt cttcaatctc catttttccg
     1921 gattcgatcc ttgaaatatc caaaatatta ttgatcaatg agatcagata acctccagaa
     1981 cgttcaatgc tttttgcata ttggcggatt tctttgcttt cactttctct aagaatcatc
     2041 tcgttcattc caagcattac attgatcgga gtccggatct catgactcat attagcaaga
     2101 aatgcactct ttgccttgtt cgctgactgt gcttcgatcc ttgcttcttc taagatctct
     2161 tgttgtttgt tgtacatccg aaaatgcaga tacatcactg ctgccaccga aatactagat
     2221 gcacaaaatc ctatcaacag atccatcaca acttcttttt cagaggagaa ccatacaacc
     2281 atctgcggat tttgataggc gatcatgatc gttgctatat atacgaccat ttccataaat
     2341 gctgtaaaaa acattacctt tccttccaac ataaagactg taaataagat tccaaaaata
     2401 taaaaagaga ccatccctcc tttgtaaccg ccggatttaa aaaataaata tgggaacatc
     2461 ataagaaaga tcataatgat cgtaacaaca taacaaaact gatatctgcc acttttgtat
     2521 gcataaaaca acaacacgaa agacaacact gcaatcccaa gattcaatcc tagaatcaca
     2581 ctatcctgat tattcgcata tgatgaaatc ccattgatca gactgatcat tccaccaact
     2641 aatgcaagca cattaaataa tcttacccga aatggaaggg ataaccgaaa cagtgaatgt
     2701 tctccctgca atatctgacg aaaccatttt cccacgaact ttcctcccct ttcatgatct
     2761 tgcttctagt ttatcacgct ctttcttatc tccctagtta ctatcagtca tctcttttgg
     2821 aatgctgttc taagaaatgc aataccgcat cataaacttt ctctacatca tcttcattta
     2881 agatctcatg tctccgattt aaaaataaca ttccacgtac ctgattatac cctcgctttt
     2941 ttaaagttgt cattgcctgt gcatactgtt tctttgaacc aatgcatgga tcatctgctc
     3001 ctgcgataaa caagatcgga agtgacggat gttttacctg ccatcctttt ttgttgtaag
     3061 tttcatccat tagtaaaaac agatttaaaa atccattatt actaaatacg aatccatcca
     3121 atggattttc atcataagct tttacatttt ctttgttata actgatccag ccattttcgg
     3181 attcatcttc tcctaatgcc tttccatata agccaagtgc catcttctgg aagaactttc
     3241 cttttttgtg tgctcccatt ttacaagcag tttttgcaag aaatcctgct gcctttgctg
     3301 ctttattttc acttggactt ccacatacga tcagagagtc cagctcatca tcatattttt
     3361 tcagataaca acgaaccacc aaggatccca tactatgtcc gaacaaatga tatggaagtt
     3421 cttccccata tcgttctttc atccatgttg ttacctgatg agcatcttct atgatcgctt
     3481 ttccactgtc atcataaaaa tacccaaggt ctgacgcatt tttgacactc ttaccatgac
     3541 ctctgtgatc atggatcaca ctgatatagc cattgttaca caacatttgc ataaaaggct
     3601 catagcgttc tttatgttct gccataccgt ggcagatctg tacgattccc tttggagatt
     3661 gttctggttc cataagcagt acatgtagtt ttaatccatc ttgttttgat gtcaggtata
     3721 ctgttttttt catttcaagt acctccgttc ctattcttat ttttagttta acacaaaatt
     3781 atgattatat ggtaaactaa acttaataac tactaactct ttcatggagg gattcattat
     3841 gaaaattaga ataaaaaaag tgactgcatt gcttctttcc tgtatgctgc ttttttcgat
     3901 cagcggctgt cagaagaaac gtactgcgaa agaagttttg gaatcaagtc ttaaacaaag
     3961 ttccaaatta aaagatgctg atttcagtgg agaagcttct tataagatcg caaatagtga
     4021 ggcttcttca tcctcaaacg ttacgatcaa gatgaatttt gacacaaaac tacagactgt
     4081 tgataaagat cagttaaaaa tgtctatgac ttctacctta aacatgcttg ggcagactat
     4141 ggatatgaat atgtattatt ctgatggtta ttattacatg aacaccaatg gaacaaagca
     4201 gaaaataaaa atggatatcg caggtcttca aaaacagatt cagtcaacca caggtcagac
     4261 ttctttgtct gcaaaatatt acaaagatct gaaacttagt gaaaaggatg gaaatactgt
     4321 tcttaaatac agtatcaata atgatggatt gaatcaatac gtcaaagatg ttacctccca
     4381 gatgacaact gtaactggtg gaagtaattc cattaagatt tcttctttga ccggtacaaa
     4441 aacattgaat gacaaagatc ttcctgtgaa agaatccatc cagatggtta tggaaagcgg
     4501 tgacaatgag acaggttcga tcactttaaa aatgaacctg acttatcatg atcctggaaa
     4561 atctgtgact gtgaccttac ctgacgatct gaatacttat caggaaattt caaattaata
     4621 aattttattg cataggaaat tccgataaaa tcctaaagca aacgacgtaa gcatcttatt
     4681 cttttgaata taatacttac gtcgttgttt tttatgatct tattttataa tttttttttc
     4741 ttatgaaata gt
//
LOCUS       DAOMUE010000151         4751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5013_length_4751_cov_3.071124, whole genome shotgun sequence.
ACCESSION   DAOMUE010000151 DAOMUE010000000
VERSION     DAOMUE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5013_length_4751_cov_3.071124"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1422)
                     /locus_tag="PLC71_07295"
     CDS             complement(64..1422)
                     /locus_tag="PLC71_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM02483.1"
                     /translation="MKMKQKILNNMVYIAVVAVLITTTLIGMFTYYRYMEQIKTGMKD
                     EAAYLAASLNFEDQQNLDKYKNITITRITRIAKDGTVIYDSSGKEKSMGNHKNRTEVK
                     EAFKHGHGEDTRMSMTLRKQTYYYALKLKDGTILRMSRETQTIVRQMEDIIPIIALIL
                     AVVTILSVILSRMSTDRIVEPINQINLIHPKQNKTYSELTPLLDKIEKQNMDIERQIK
                     EIKEAENMRKEFSANVSHELKTPLTTISGYAELMKDGLVKPEDMPRFSATIYDEARRL
                     ISMIEGIIKLSRLDENRVELDWKDVDLYELAFSIKNDLKRRSEEENVAIHIRGIHTKI
                     RGVTQILYEMFFNICENAIKYNHPGGEVVFTISKMGDYPTVIVEDTGIGIPKEDIDRI
                     FERFYRVDKSHSNQKEGSGLGLSIVKHGAKFHHAEMEVESEVGKGTKITVVFPRQNSD
                     LL"
     gene            complement(1419..2099)
                     /locus_tag="PLC71_07300"
     CDS             complement(1419..2099)
                     /locus_tag="PLC71_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM02484.1"
                     /translation="MIFVVEDDPNIRELVTYTLQSTGFDACGFENGSEFLKALSDGEK
                     PELVLLDIMLPGEDGISILGKLRKKSATRDLPIIMMTAKGTEYDKVLGLDSGADDYIT
                     KPFGMMELVSRVKAVLRRSSRNEKKDEIIVGQLKIYPNKHKVKSCGNEVTLTNKEFKL
                     LCLLVESKGNVLNRDQLLTNIWGYDFDGETRTVDVHIRSLRQKLGECGKLIETVRGIG
                     YRIGGNES"
     gene            complement(2096..2749)
                     /gene="phoU"
                     /locus_tag="PLC71_07305"
     CDS             complement(2096..2749)
                     /gene="phoU"
                     /locus_tag="PLC71_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRM02485.1"
                     /translation="MRNRFDRQLLRLNQEMIDMGSLCEEMITAAAKIVATRDKELMDD
                     IVTMEEEINQKERDIERICMKLLMQQQPVANDLRVISAAMKMVSDMERIGDQACDITG
                     IVKKDAEVLTIRENTHLDKMAKATISMVSKSIQSFIKQNLVIAHEVIEQDDIVDELFN
                     RVKKETISLIHEDPGKGEECVAIVMIAKYFERIGDHASNIAEWVEFAITGEYKGGSL"
     gene            complement(2755..3507)
                     /gene="pstB"
                     /locus_tag="PLC71_07310"
     CDS             complement(2755..3507)
                     /gene="pstB"
                     /locus_tag="PLC71_07310"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRM02486.1"
                     /translation="MDSKMKVENLDLYYGKFKALKNINLDLPKNEITAFIGPSGCGKS
                     TLLKSLNRMNDLVEGCKITGKVTLDGDDIYGDMDVNLLRKRVGMVFQKPNPFPMSIYD
                     NIAYGPRTHGIRSKAKLDDIVEKSLRNAAIWDELKDRLKKSALGLSGGQQQRLCIARA
                     LAVEPEVLLMDEPTSALDPISTSKIEDLAVELKSKYTIIMVTHNMQQAVRISDNTAFF
                     LLGEMVEYGKSDQLFSMPQDKRTEDYITGRFG"
     gene            complement(3514..4416)
                     /gene="pstA"
                     /locus_tag="PLC71_07315"
     CDS             complement(3514..4416)
                     /gene="pstA"
                     /locus_tag="PLC71_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRM02487.1"
                     /translation="MEAVKEMESVEKIQAINKTSWIQRMKSYKRTPLSFVLWLLVNVA
                     MIATVAVLAYLIVYILVNGVPYLNSQLFELKYTSENASVFPAIINTLIMTVLSLVLAV
                     PLGIFAAIYLVEYAKRGNKLVEVVRLTAETLSGIPSIVYGLFGMLFFVKALGWGLCLL
                     SGAATMAIMILPLIMRTTEEALKSVPDSFREGSFGLGAGKLRTVFCIILPSAVPGILS
                     GVILGIGRIVGETAALMYTAGTIAEIPANVMGSGRTLALHMYVLSCEGLHINQAYATA
                     VVLLLIVLVINAVSSFIAKRITKG"
     gene            complement(4418..>4751)
                     /locus_tag="PLC71_07320"
     CDS             complement(4418..>4751)
                     /locus_tag="PLC71_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRM02488.1"
                     /translation="GSLGLGASHERSVFFAVLPAAKSGILAGIVLGIGRAIGETMAVV
                     MVAGNQAIIPDGMFSGVRTLTSNIVLEMGYATDLHREALIATAVVLFVFILMINLCFA
                     VLKSKEER"
BASE COUNT         1277 a         1066 c          727 g         1681 t
ORIGIN      
        1 aaaatgcgga gtgtcatcct ttttcttata aatatttctt ctttctgact catacatgca
       61 acactacaac aaatcagaat tttgccgtgg aaatacaacc gtaatctttg ttccttttcc
      121 cacttcactt tctacttcca tctctgcatg atggaatttt gctccgtgtt ttacaatgga
      181 taatccgagt cctgagcctt ctttttggtt tgaatggctc ttatctacac gataaaaacg
      241 ttcaaatata cgatcaatgt cttcttttgg aataccaatt ccggtatctt ctacaataac
      301 tgttgggtaa tcgcccattt ttgaaatcgt gaatacgact tctccgccag gatgattgta
      361 tttaatcgca ttttcacata tattaaaaaa catttcgtaa aggatctgtg taactcctcg
      421 gatcttcgta tgaattccgc ggatatgaat agctacattt tcttcttcac ttcttcgctt
      481 cagatcattc ttgatactga atgctaattc atagagatcg acatctttcc agtctaattc
      541 cactctattt tcatcaagtc ttgagagttt gatgattcct tcgatcatcg agatcaatct
      601 tcttgcttca tcatagatcg ttgcagagaa tcttggcatg tcttctggtt tcaccagccc
      661 atctttcatt aattcagcgt atcctgaaat cgtagtcaat ggagttttta gttcatggga
      721 tacattggct gaaaattctt tgcgcatatt ttctgcttct ttgatctctt tgatctgtct
      781 ttctatatcc atattctgtt tttcaatctt atcaagcaat ggcgtcaact cactatatgt
      841 tttgttctgt tttggatgta tcaaatttat ctggttgatc ggttctacga tacgatctgt
      901 cgacattctt gataatatca cagataatat cgttacaact gcaagaatca aggcgatgat
      961 cggaatgata tcttccatct gtcttacgat cgtctgggtc tcccggctca tccgaagaat
     1021 cgttccatct tttaacttca gcgcataata ataagtctgt tttcgtaatg tcattgacat
     1081 acgagtatct tccccatgtc catgtttaaa tgcttctttg acttctgtac gattcttatg
     1141 gtttcccatg gatttttctt tcccgctgct atcataaata accgttccat ccttagcgat
     1201 ccttgtaatc cttgtgattg taatattttt atatttatcg agattttgct gatcctcaaa
     1261 attaagtgaa gccgccaaat aggccgcctc atctttcatt cctgttttta tctgttccat
     1321 ataacgataa tatgtaaaca ttccaataag cgttgtcgtg atcagcacag ctacaactgc
     1381 gatgtatacc atgttgttta atatcttttg tttcattttc atgattcgtt gcctccgatc
     1441 cggtaaccga ttcctctgac cgtctcgata agctttccac attctccaag cttctgcctt
     1501 aatgaacgaa tatgaacatc gacagttctt gtctcaccat caaaatcata tccccagata
     1561 tttgtaagca gctgatctct gtttaataca tttcctttac tctctaccaa taaacataat
     1621 agtttaaatt ccttattggt taatgtcact tcattcccac aggattttac tttatgttta
     1681 tttggataaa tctttaattg tccaacaatg atctcgtctt ttttttcatt tctgctggaa
     1741 cgtctcaata ccgctttgac tctggataca agttccatca ttccgaatgg ttttgtaata
     1801 taatcatctg ccccactatc aagtccgagt actttatcat actctgttcc ctttgctgtc
     1861 atcataatga tcggcagatc tctggtcgct gattttttac gaagttttcc taaaatcgat
     1921 attccgtctt ctcctggaag cataatatct aataatacca gttctggctt ctctccatca
     1981 cttaaagctt ttaaaaactc tgatccattt tcaaatccac aggcatcaaa tccggtactt
     2041 tgtaaggtat atgtcaccag ttctctgata ttcggatcgt cttctacaac aaaaatcaca
     2101 agcttcctcc tttatattcc ccagtgatcg caaattcgac ccattctgcg atattgcttg
     2161 catgatctcc gattcgctca aaatacttgg cgatcatgac gatggctaca cactcttctc
     2221 ctttcccagg atcttcatga atcaatgaga ttgtttcttt cttgactctg ttaaataatt
     2281 catcaacaat gtcatcttgt tcgatcactt catgagcgat caccagattc tgtttaataa
     2341 aagattgtat gctcttgctt accatactga tcgtagcttt tgccatctta tcaagatgtg
     2401 tgttctctct gatcgtaaga acttctgcat cttttttaac gattccagta atatcacatg
     2461 cctggtctcc gatacgttcc atatcagata ccatcttcat agctgctgag ataacacgca
     2521 gatcgtttgc cactggctgt tgctgcatca gaagtttcat acagatacgt tcgatatctc
     2581 tttccttctg attgatttct tcttccattg ttacaatatc atccatcagt tctttatctc
     2641 ttgtcgcaac gatcttggcc gcagctgtga tcatctcttc acataaagaa cccatgtcga
     2701 tcatctcttg atttaaccta agcagctgtc tgtcgaatcg atttctcatc taaatcaccc
     2761 aaaccttcct gtaatataat cttctgttct cttatcctgt ggcatagaaa atagctgatc
     2821 tgattttccg tactctacca tctctcctaa taagaagaat gctgtattat cagaaattct
     2881 gactgcctgc tgcatattat gtgttaccat gatgatcgta tactttgatt ttaattctac
     2941 cgcaagatct tcaattttgg aagtagaaat tggatcaaga gctgacgttg gttcatccat
     3001 tagtaaaact tcaggttcta cagctaacgc tctcgcgata caaagtctct gctgctgacc
     3061 tccagaaagc cctaaagcgc ttttctttaa gcgatctttt aattcatccc aaattgctgc
     3121 atttcttaaa gatttttcta cgatatcatc taattttgct ttgcttcgga ttccatgagt
     3181 tcttggtcca tatgcaatat tgtcataaat actcattgga aatggatttg gtttctggaa
     3241 taccattcca actcgttttc ttaataaatt gacatccatg tcaccataaa tatcatctcc
     3301 gtctaatgtt actttacctg tgatcttaca accttctaca agatcattca ttcggtttaa
     3361 tgattttaaa agtgtagatt taccacatcc agatggtccg ataaatgctg tgatctcatt
     3421 tttaggaaga tccagattaa tattttttaa agccttgaat tttccataat acaggtcaag
     3481 gttttcaact ttcattttac tatccataat tggttaccct ttcgtaattc gttttgcgat
     3541 aaatgaagat acggcgttga tcacaagcac gatcagaagt aacacaactg ctgttgcata
     3601 tgcctgattg atatgtaatc cttcgcatga taatacatac atatgaagtg ccagtgtacg
     3661 tcctgatccc ataacatttg cagggatttc tgcgatcgta cctgctgtat acattaatgc
     3721 tgccgtctca ccgacgatac gtccgattcc taaaataact ccagataaga ttcctggaac
     3781 tgctgatggc aggataatac aaaatactgt tcttagtttt cctgctccaa gtccaaagct
     3841 tccttctcta aaagaatcag gcacagactt taatgcttct tcggttgttc tcatgatcaa
     3901 tggtaagatc atgatcgcca ttgttgctgc tccagatagc agacataatc cccatcccaa
     3961 agcttttacg aaaaataaca taccgaataa tccatacacg atcgatggaa ttcctgataa
     4021 tgtttctgct gttaaacgaa caacctcaac taatttattt ccacgttttg catactctac
     4081 taaataaatt gctgcaaaaa ttccaagtgg tactgctaat accaaagata atactgtcat
     4141 gatcaaggta ttaatgattg ctgggaatac cgatgcattt tcacttgtat attttaattc
     4201 aaataattgt gaatttaagt atggtactcc atttacaaga atataaacga tcaaatatgc
     4261 aagtactgcg acagttgcga tcatcgcaac atttacaaga agccataata caaaggataa
     4321 cggcgttctt ttatatgatt tcattctttg tatccaagac gttttattga tcgcttggat
     4381 cttttctact gattccattt ctttcaccgc ttccatttta acgttcctcc ttgctcttta
     4441 atactgcaaa gcacaggttg atcattaaga taaatacaaa taatacaact gctgttgcga
     4501 tcagtgcttc tctgtgaagg tctgtcgcat atcccatttc aaggacgata ttggatgtca
     4561 aagtacgaac tcctgagaac attccatctg ggatgatcgc ctgatttcca gctaccatta
     4621 ctaccgccat tgtctcaccg atcgcacgtc cgattcctaa aacgatccct gcaagaatac
     4681 ctgattttgc tgcaggtaac actgcgaaga atacgcttcg ctcatgactt gctccaagtc
     4741 ctaaggaccc t
//
LOCUS       DAOMUE010000152         4750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5014_length_4750_cov_2.443024, whole genome shotgun sequence.
ACCESSION   DAOMUE010000152 DAOMUE010000000
VERSION     DAOMUE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5014_length_4750_cov_2.443024"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLC71_07325"
     CDS             complement(<1..847)
                     /locus_tag="PLC71_07325"
                     /inference="COORDINATES: protein motif:HMM:NF026238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding Tp47 domain C-containing
                     protein"
                     /protein_id="HRM02489.1"
                     /translation="MKKIKVDMTSLLVQLQITDFTEEVQGNAVIKAKASDDTTKEFSV
                     SHWSKDGKKFYQSSTDATGVSYAKGTITDTDGTQYKLTSYEVKGTKYVPVKVKTTDLD
                     VLKANHTVVENGGTLVGGYGENQLKSYQKTANVTANTYGLKTATKQEDGSFIFSARSN
                     EGTESGVKDEALKEATGVTPNLRSGDAVGSYGETLRLDLNGDYGDLGANLQSVKWTFY
                     GDDSTYTTPVKTYGTKFAADNWMHKSMGIQLGLTESARFDLSGDAAVGYWSVTVSALG
                     YKDYTY"
     gene            complement(756..1088)
                     /locus_tag="PLC71_07330"
     CDS             complement(756..1088)
                     /locus_tag="PLC71_07330"
                     /inference="COORDINATES: protein motif:HMM:NF026239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding Tp47 domain A-containing
                     protein"
                     /protein_id="HRM02490.1"
                     /translation="MRKRLIGKAFAIAMSGAMVLTATPVTANIFNVAHIVKAADDAQQ
                     DEWTYCYVGLTWAEYWANEGVYNANDTSSSDVEDSHYEKDKGGYDVVTRATTNHGLHR
                     GSPRKCSH"
     gene            1399..2106
                     /locus_tag="PLC71_07335"
     CDS             1399..2106
                     /locus_tag="PLC71_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRM02491.1"
                     /translation="MNQPKILVVDDDKEIVGAIKKRLEMEDYEIITAYDGLEAMDILM
                     EQEVQLLIIDVMMPKMDGLSATMKIRESKNIPIIILSAKTEESDKILGLSMGADDYIS
                     KPFRPDELVARVKSQLRRYMQFGGMNAAESNKEQIINGGLILDMKGKRMMVDGEPVKL
                     TATEYKIVSLLMNNLGRVFSADEIYERVWNEEAYATENTVMVHIRRIREKIEIDAKNP
                     RYLKVVWGIGYKIEKMD"
     gene            2078..4231
                     /locus_tag="PLC71_07340"
     CDS             2078..4231
                     /locus_tag="PLC71_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRM02492.1"
                     /translation="MDTRSRKWTNKVLITLVMIAMTLSGIYGLNMIHNMALTGDNSFS
                     LDEQDQSDYIKHTQMSLFDKWADQESNKVYYTQANHVSESLLNKIKKESFVKVTMKVK
                     PYDKKMVTTTSTYGTENANYTGGRIEHFHIERDKTEKLNKDGSFKGWDPYDDYDEYSE
                     YIVPQTEYYEFSGDNYTLRDIFPEQLLINDEKNQRLENADIEISLTKKALTEIENFRK
                     GSTKQITTGIKTIIVVSIIEVIGTILLCILFALQEKRTRFFRGLDRLWWEIILVTATT
                     LLGLAIALCFAIWEYTIEYTQGGITGSTIEMIMTLSYISVPFFIMAFAVCIQTTVWRI
                     RQKNLLDSTFCLGYIRKWSRQAKTRRKLEDEAMSFAEKHARDRIRQYRVLRYILTVGT
                     LACIIMGMSGMNIPSPLLFIAAVIFVFLIRYFSRYSDKYAKEQRDLSMLVDQIERISN
                     GELTATTDISKESAYYEYSQKLTNIGQGMEKALETQMKGERMKIDLITNVSHDLKTPL
                     TSIIGYVDLLSRDQTLSAESRDYVTILINKTERLKSIISDLFELAKATSGDAKVDLEE
                     MDMKRLVEQTLGDMADQIEESGFAIRYQCEATHTRFIGDSSRMYRVVQNVIENALKYS
                     LKGTRIFLRITEADQNIKLEVINTASYEMNFTEEEIMERFARAEKSRTSEGNGLGLSI
                     ADSFTKNCGGEFSIRIDGDQFLVTIQFKQHLDVKK"
BASE COUNT         1640 a          875 c          973 g         1262 t
ORIGIN      
        1 tatatgtata atctttataa cctaaagcgg aaacagtaac actccagtat cctactgctg
       61 catctccaga aagatcaaaa cgagcagatt ctgttaaacc aagctgaatt cccattgatt
      121 tatgcatcca gttatcagca gcaaattttg taccataagt ttttactgga gttgtataag
      181 tactgtcatc tccatagaaa gtccatttta cagactgaag attagcacca agatcaccgt
      241 aatctccatt taggtcgaga cgtaaagtct caccataaga accaacagcg tctccagaac
      301 gtaagtttgg agtcacaccg gttgcttctt ttaatgcctc atctttaaca ccggattctg
      361 ttccttcgtt acttcttgca gaaaaaataa atgaaccatc ttcctgtttt gtagcagttt
      421 ttaaaccgta agtgttagct gtaacatttg cagttttttg gtaagatttt aattggtttt
      481 caccataacc accaacaaga gttccaccat tttcaacaac agtgtggttt gcttttaata
      541 catctaaatc tgtagttttt actttaacag gaacatattt tgtacctttt acttcataag
      601 aagttaattt atattgcgta ccatcagtat cagtaatagt tcccttagca tagcttacac
      661 ctgtagcatc tgtggatgat tgatagaatt ttttaccatc tttactccag tgagaaacag
      721 aaaattcttt tgtggtatca tcagaagctt ttgctttaat gactgcattt ccttggactt
      781 cctctgtgaa gtccgtgatt tgtagttgca cgagtaacga cgtcatatcc acctttatct
      841 ttttcataat gagagtcttc tacatcagag cttgaagtat cattagcatt ataaacacct
      901 tcattcgccc aatactcagc ccaagtaaga ccaacatagc aatatgtcca ttcatcctgc
      961 tgtgcatcat cagcagcctt aacaatgtga gcaacattaa agatattcgc tgtcacagga
     1021 gtcgcagtta aaaccattgc accactcata gcgatagcaa aggctttccc gattaatctt
     1081 ttacgcatat agtttttcct ccgttttctg taatataaaa taatagcgtg tacgtagtta
     1141 gaaaactcta actgatgaaa gatatgatac cacacgaaag gttagtgaac aataactaca
     1201 aatgagaata attatcaata tcttgtcatt cattaaatgc aggtgtgctc ctacggtcac
     1261 tggacaggat attgataatt attctggaag ttactataat taaggaagtc atattaagaa
     1321 attcgtaaga attatatccg cagaaacgaa agaaccacct gatacactaa aaccatcaaa
     1381 gatacgagga ggaaaagtat gaaccagcca aagatcctgg tcgttgacga cgacaaagag
     1441 atcgtaggag cgatcaagaa acgtctggaa atggaagact atgaaatcat aacggcatat
     1501 gatggtctgg aagccatgga tatcctgatg gaacaagaag tccagcttct gatcatagac
     1561 gttatgatgc cgaagatgga cggactatcc gcaacgatga agatcaggga aagcaagaac
     1621 atcccgatca tcattctttc agccaagaca gaagaaagcg ataagatcct gggactatct
     1681 atgggagccg acgactatat cagcaaacca ttccgcccag atgaactagt agcgagagtg
     1741 aaatcccagc tgcgaagata catgcagttt ggaggaatga acgccgcaga aagtaataag
     1801 gaacagatca tcaatggcgg actgatcctt gacatgaaag gaaaaaggat gatggtagac
     1861 ggagagccag tgaaactgac tgcaaccgaa tacaagatcg taagccttct gatgaacaac
     1921 ctgggcagag tattttcagc agatgagatc tacgaacgcg tatggaacga agaagcctat
     1981 gcaacagaga ataccgttat ggtccacatc cgtcgtatcc gcgagaaaat agaaatagat
     2041 gcaaaaaatc caagatattt aaaggtggtg tggggaattg gatacaagat cgagaaaatg
     2101 gactaacaaa gtattgatca cacttgtgat gatcgctatg accctgtcag ggatatatgg
     2161 actcaacatg atccataaca tggcattgac aggagataac agcttcagtc tggatgaaca
     2221 ggatcaatca gactatatca aacacacaca gatgtcactg tttgacaaat gggcggatca
     2281 agaaagtaat aaagtctatt atacacaggc aaatcatgta tcagaaagtt tactgaataa
     2341 gataaaaaaa gaatcgtttg tgaaagtaac gatgaaggtc aaaccatatg ataaaaagat
     2401 ggtaacgaca acatcaacat atggaacaga aaatgcgaac tatactgggg gcaggattga
     2461 acattttcat atagaaagag acaagacaga gaaattaaac aaagacggaa gtttcaaagg
     2521 atgggatccg tatgatgatt acgatgagta cagtgagtac attgtgcctc agacagaata
     2581 ctatgaattc tcaggagata attatacact gcgggatata ttcccagaac agcttctgat
     2641 caatgatgag aagaatcagc gtctggaaaa tgcagacatt gagatttctt tgacaaaaaa
     2701 agcactaaca gagattgaaa atttccgaaa aggatcaaca aaacagatca cgacgggaat
     2761 caaaacgatc atcgttgttt cgatcatcga agtgatcgga acgatccttc tgtgcatctt
     2821 atttgcactt caggaaaagc gaaccagatt ttttagaggg cttgacaggc tctggtggga
     2881 gatcatacta gtgacagcaa ctacgctgct tggactggcg atcgcattat gctttgcaat
     2941 ctgggaatat acgatagaat atacacaggg cgggatcaca ggttcaacga tcgagatgat
     3001 catgacgctg agttatatct cagtcccatt ttttatcatg gcatttgccg tatgtattca
     3061 gactacggta tggaggattc gccagaagaa tcttctcgat tcaacatttt gtctgggata
     3121 tattcgaaaa tggagccgtc aggcaaagac aagaagaaag ctggaagatg aagcgatgtc
     3181 atttgcagaa aaacatgcaa gagacaggat cagacagtac agggtcttac gatatatttt
     3241 aactgtcggt acacttgctt gtatcatcat gggaatgagt ggaatgaata ttccatcccc
     3301 actcttgttt atagcagcag tcatctttgt ttttcttatc agatattttt caagatattc
     3361 agacaaatac gcaaaagaac agagagattt aagcatgctt gtcgatcaga tcgaacgtat
     3421 ttccaatgga gaactgacgg caacgaccga tatttcaaaa gaatctgcgt attatgaata
     3481 cagtcagaaa ctgaccaata tcgggcaggg aatggaaaaa gcattagaaa cacagatgaa
     3541 gggcgagaga atgaagatcg atctgatcac aaatgtatca catgatctga agacaccgct
     3601 gacttctatc atcggatacg tcgatctctt aagcagagat cagacattat ctgcagaatc
     3661 aagagattat gtaaccatcc tgatcaacaa gaccgaacgc ttaaaatcta tcatttctga
     3721 cctgtttgaa cttgcgaaag caacaagtgg agatgcgaaa gtagatctgg aagaaatgga
     3781 tatgaaacgt ctggtagaac agacactggg ggatatggca gatcagatag aagaatctgg
     3841 atttgcgatc agatatcagt gcgaagctac acacacaaga tttatcggag acagcagccg
     3901 catgtaccga gtggtacaaa atgtcataga gaatgcctta aaatactccc tgaaaggaac
     3961 cagaatcttc ctaaggatca ccgaagcaga tcaaaatata aaattagaag ttataaacac
     4021 agcatcctat gagatgaatt ttacagaaga agagatcatg gaacggtttg ccagagcgga
     4081 aaaatcacgt accagcgaag gaaacggatt aggactttcc atcgcagaca gttttacaaa
     4141 gaattgtgga ggagagtttt cgataagaat agacggagat caattcctgg ttactataca
     4201 atttaaacaa catttagatg taaaaaaata gatttcatat atttttttac aaaattgtaa
     4261 aaattgaaaa aaactatgtt gaaagggtag aaaacataaa tataaaagta tatactagtg
     4321 gtgtcgacaa tatgcaaata gaccatttga gagagtggtt tttaaatcct ttttttcatg
     4381 aagttttgat acatggtgac ttatggaacg gttgcgtatt tggagacatc tgcttttaaa
     4441 gtctcttcag acattaagat ctggaggggc ttttttctta tttcatagga aatttcgatg
     4501 aaatccccta tgaaataaga aaaaagccct ccgggcagga tcgcactgcg gcggaaggga
     4561 aatttgtcgc tgaagcgacc cgccgcaggc ggagaatcct gcaaagcagg attctttctt
     4621 atatcgaaat taaggattca aaatattctt cacaacatcc ttatcgctca agtaaaccat
     4681 atgtgatcct ttcagctgta cataataatt cttttgatca gaatcttttt taccgatata
     4741 aagcacaaca
//
LOCUS       DAOMUE010000153         4750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5015_length_4750_cov_2.377636, whole genome shotgun sequence.
ACCESSION   DAOMUE010000153 DAOMUE010000000
VERSION     DAOMUE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5015_length_4750_cov_2.377636"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLC71_07345"
     CDS             <1..468
                     /locus_tag="PLC71_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02493.1"
                     /translation="LADNHSDLYDYCMSTNQSSCYQLMTQRTATFLQYYYPNPLEFAQ
                     NSRHYKCQKGNAIGFMYYPTGEGNDGYKILEGFVNKELLADWKSVAYTEREKELLENS
                     RKYYTNSVNTVNSMKSYYEIKPSYDSIKNIEGGKLSKEVAKGAVDYLFVWELD"
     gene            474..1922
                     /locus_tag="PLC71_07350"
     CDS             474..1922
                     /locus_tag="PLC71_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRM02494.1"
                     /translation="MEYSEQLSMDAQCKSTYTVYLAKNNIKNSSPHNPPKVEGLSDEY
                     YSKCQSGNGENLYSCGIISTSIIDSISSALDDSQGTGQYYNRGHRYNLLNPEWKYIGV
                     GNTLQQACHKLSGTQSSNVDVVAWPPKGITISESGFSPSGMWTCQFYNKLKPTADTTI
                     TIECLNSNKKWKIDPNNLLENQDYNYERSGDLISYSDDSIVFKIGGVYQITYDHLTDA
                     NGNETSYSYRTVYEKAYIGSEEEKVPQSIKLDKTSEKVLLGTTSKLIAKVSPDNIKNK
                     RIYFASNNKEVATVNECGEITAHSLGTADITATSENGNITATCKIIVVKTLDQTDDQP
                     EKEPTKEPEKEPTGAITNGNVNNSNNKSDLSQGHKTNNSKKENITISKAKIKSAKKKK
                     SSKSLTIILSKAVPKVIGYQIKIYSSKKKAKKNKGAIWTKVVQTNSKKIVIKNKKLKN
                     KKTLYIRIRAYKRINRKNKYSTWSEIKKVIVN"
     gene            1998..2192
                     /locus_tag="PLC71_07355"
     CDS             1998..2192
                     /locus_tag="PLC71_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM02495.1"
                     /translation="MEYLMYTDIPVIKIAMNNGFASVVAFNKVFKETYQETPSAYRKK
                     KKETVDNQESMLENYILQSG"
     gene            complement(2298..3785)
                     /gene="panF"
                     /locus_tag="PLC71_07360"
     CDS             complement(2298..3785)
                     /gene="panF"
                     /locus_tag="PLC71_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015498 - pantothenate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HRM02496.1"
                     /translation="MSANQKIILAIFIVYLALNAAIGIIFSNRQNKKQHTSSEKKFFI
                     GGRNMNGLLLAMTTMATYTSVSSFISGPGAAGLTYGYAQAWIATVQVPITFLVLGVLG
                     NKLALVSRRTGAVTVVGYLKARYKSDVLVIVTSLLMVAFFMAQMIGQFTGGATLISSI
                     TGLDHVVSLLIFGAVVIIYTSFGGFSAVAITDTIQGIIMCVGTFLLLFFVLKAGGGLA
                     GIDAGLAQNLPGVYDDLFSKYSPGTLLSFWILVGFGTLGLPQTAVRSMGFKNTKSLHS
                     AMWIGAVTCSFIIVGMHMAGTWAGALVDTKNLPTSDYFIPYIVQKIMPVGLAGTFLAA
                     PMAAVMSTADSLLILASAAIVKDLWRNYVVKDDPVKIQSYNKNVGKMSTLVTFAFGVI
                     VILLTINPPDIIFFLNLFALGGLECSFFWPLVGGLFWKKGTKQAAVFSSLGAAITYVF
                     CYYNVQIAGINAVVWGLLAGGILYFVIGNITCKNGLDEDIIKNCF"
     gene            complement(3786..4052)
                     /locus_tag="PLC71_07365"
     CDS             complement(3786..4052)
                     /locus_tag="PLC71_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HRM02497.1"
                     /translation="MKKLTRQEKHEQCMREIRGTLIVVLICCAWHIASAFLLNGTGLY
                     FLGMPAWFSVSTFGTIILSLIGVWYLLKHVFINFEYDDEEEGEE"
     gene            4258..4632
                     /locus_tag="PLC71_07370"
     CDS             4258..4632
                     /locus_tag="PLC71_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HRM02498.1"
                     /translation="MENKSSYQINEQGVLGEVKIADDVIAVIAGIAATEVTGVARMCG
                     NITNELVSKLGVNNLSKGVKVVVENHHVRVNLALELDYGVSIPEISKKVQDRVKAAIE
                     NMTGLKVREVNVRVTGVAAEEE"
BASE COUNT         1830 a          756 c          903 g         1261 t
ORIGIN      
        1 ctggcagata atcatagtga tctatacgat tattgtatga gtacaaatca aagttcttgt
       61 tatcagttaa tgacccaaag aacagctaca tttctgcaat attattaccc taatccatta
      121 gagtttgcgc aaaatagtag acattacaaa tgccaaaaag gaaatgcaat aggttttatg
      181 tattatccaa caggagaagg aaatgatggt tataagattt tagaggggtt tgtaaataaa
      241 gaattattgg cagattggaa gtctgttgca tatacagaaa gagaaaaaga gttattggaa
      301 aattccagaa aatattatac aaactcagta aacacagtta attctatgaa atcatattat
      361 gagattaaac cttcttacga ttcaattaag aatatagaag gtggaaagtt aagtaaagaa
      421 gttgcaaaag gagcagttga ttatttattc gtgtgggagc tggactaaat ccattggaat
      481 attcagaaca attgtctatg gatgcacagt gcaaatctac gtacacggta tatttggcaa
      541 aaaataatat aaaaaattca tcacctcata atccaccaaa ggtagaggga ttaagcgatg
      601 aatattattc aaaatgtcaa agtggcaatg gagaaaattt atactcatgt ggaataataa
      661 gtacgagtat catagacagt attagtagtg cattagatga tagtcaaggg acaggacaat
      721 attataatag aggtcataga tataatctgt taaatcctga atggaaatat attggagtag
      781 gaaatacgtt gcaacaagca tgtcataaat tgtcaggaac gcagagttct aatgtagatg
      841 ttgttgcatg gccaccaaaa ggaattacta tttcagaaag tggattttcg ccatcaggaa
      901 tgtggacatg tcagttttat aataagttaa aaccgacagc tgatactaca ataacgattg
      961 aatgtctcaa tagcaataag aaatggaaga ttgatccgaa taatctttta gaaaatcaag
     1021 attataatta tgaaagaagt ggggatctta tttcctatag cgatgattct attgttttta
     1081 aaataggagg agtttatcaa ataacatatg atcatcttac agatgcaaat gggaatgaaa
     1141 caagttattc ttatagaaca gtgtatgaaa aagcttatat tggatcagaa gaggagaaag
     1201 taccacaatc tataaaatta gataaaacat cagaaaaagt tttgcttggt acaacgagta
     1261 agttgattgc caaagtaagc ccggataata taaagaataa aagaatttat tttgcaagta
     1321 ataacaaaga agtggctact gtaaatgaat gtggtgagat tacagcccat tcgttaggaa
     1381 cagctgatat aacagctaca agtgaaaatg gaaatataac agctacatgc aaaataatag
     1441 tagtaaaaac tttagatcag acagatgatc agccagaaaa ggaaccaaca aaggaaccag
     1501 aaaaggaacc aactggagca ataacaaatg gaaatgttaa taatagtaat aacaagtctg
     1561 atttatcaca aggacataaa acaaataaca gcaaaaaaga aaatataacc attagtaaag
     1621 caaaaattaa aagtgcgaaa aagaaaaaat cctcaaagtc cttaacaata atattatcaa
     1681 aagcagtacc taaggtaatt ggttatcaaa taaaaatata ttcatccaaa aagaaagcta
     1741 agaagaataa gggtgctatt tggacaaagg ttgtacagac aaattccaag aaaatagtta
     1801 ttaaaaataa aaaattaaaa aacaaaaaaa cactttatat tcggatacgt gcttataaga
     1861 gaatcaatag aaagaataaa tatagtacgt ggtcagagat taagaaagta attgttaatt
     1921 agtaatgatt tcagtgaatc attacgagtg ttgcttcttt caagctaaaa taaagacaga
     1981 aaataataac tcatgcgatg gaatatttaa tgtacacgga cattccggta attaagattg
     2041 cgatgaataa tggatttgct tcagttgtag catttaataa agtatttaaa gaaacatatc
     2101 aagaaacacc atctgcatat cgaaagaaaa agaaagaaac agtagacaat caggaaagta
     2161 tgttggagaa ttatatattg caatcaggat gaaaatgata aaatccctca agtatgatat
     2221 taataagaag ttttatatat catacatggg ggatttttaa tgtgtattgc ttttaagata
     2281 tttctatgct ttaaaattta gaaacaattt ttgatgatat cttcgtcaag accattttta
     2341 caagtgatat ttcctatcac aaaatataag attccaccag caagaagtcc ccatacaacg
     2401 gcattgatcc cagcaatctg tacgttataa taacaaaata cataagtgat cgcagcaccg
     2461 agagaactaa agactgctgc ctgttttgtt cctttcttcc agaataatcc tccgaccaaa
     2521 ggccagaaga agctacattc caggccgcct aaagcaaaaa gattcaggaa gaaaatgata
     2581 tcaggtggat tgatcgtaag taagataacg atcacaccaa atgcgaacgt aacgagcgtg
     2641 ctcattttcc caacattttt gttataagac tgaattttca caggatcatc tttgacaaca
     2701 tagttacgcc atagatcttt cacgatcgct gcagaagcaa ggatcaagag agaatcagcg
     2761 gtagacataa cagcagccat aggagcagct aggaaagttc ctgcaagtcc gactggcatg
     2821 atcttctgaa cgatgtatgg gataaagtaa tcagacgtcg gaaggttctt agtatctacc
     2881 agtgcaccgg cccatgtacc agccatatgc ataccaacga tgataaaact gcaggtaaca
     2941 gcaccgatcc acatcgcaga atgaagactc tttgtatttt taaatcccat agaacgaacg
     3001 gctgtctgag gaagtccaag tgtaccaaat cctacaagaa tccagaaact aagtagcgta
     3061 ccgggactgt actttgaaaa tagatcatca tatactccag gcagattctg tgccagtccg
     3121 gcatcaatac cagcaagtcc gccgccagct tttaatacaa agaataaaag taaaaatgta
     3181 ccgacacaca tgataattcc ctgaatcgta tctgtgatcg caacagcgct gaaaccacca
     3241 aaagatgtat aaatgataac aactgctcca aagatcaata aagaaacaac atgatcaagt
     3301 ccagtgatag aagagatcaa agttgctccg ccagtaaatt gtccgatcat ctgtgccatg
     3361 aagaaggcaa ccataagtaa gctggttaca ataactaaaa catcactttt ataacgagcc
     3421 tttaaataac ctacaacagt aacggcacca gttcttctgg aaactaaagc aagtttgtta
     3481 ccaaggacac ctagtaccag aaatgtgatc ggaacttgaa ctgtagcgat ccatgcctgt
     3541 gcatatccat aagtaagacc agcagctcct ggaccagaaa taaaggaact tacagaagta
     3601 taagttgcca tggttgtcat tgccagaaga agtccgttca tatttcttcc accgatgaag
     3661 aattttttct cggaggatgt atgctgtttt ttattctgac ggttactgaa aatgataccg
     3721 atcgcagcat ttaaggcgag gtaaacaata aaaattgcaa gaatgatttt ctgatttgct
     3781 gacatttatt cttctccctc ctcttcatcg tcatattcaa aattaataaa tacatgtttt
     3841 aataggtacc agacaccgat caaagaaagt atgatcgttc cgaatgttga cacactaaac
     3901 catgcgggca tacctagaaa ataaagacca gtaccattta acaaaaacgc actggcaata
     3961 tgccaagcac agcagatcaa tacaacgatc agtgttccac ggatctcacg catacactgt
     4021 tcatgtttct cctgtcttgt taattttttc atattatatg atctccttta ataaaaggtc
     4081 agattcgcaa aggatatctg ccaaaagtat tatcttacaa atatggaaga aaatcaatta
     4141 ttcttaacat tttaaagaga taagatcaaa aagaatgtta taaaatagtt tgaataacat
     4201 attcatcttt ggtataataa atcatatcag tatgcagaaa tacaggaggc ttttactatg
     4261 gagaataaaa gtagttacca gataaacgag cagggagtgc tcggggaagt gaaaattgca
     4321 gatgatgtga tcgcagtgat cgcaggaatt gcagcaactg aagtgacagg agttgccaga
     4381 atgtgtggga atatcacgaa tgaactggta agtaaacttg gagtgaataa tctgtccaag
     4441 ggagtcaaag ttgtcgtaga gaatcatcat gtaagagtca atcttgcatt agaacttgac
     4501 tatggagtta gtattccaga gatttctaag aaagttcaag atcgtgtgaa agcagcaatc
     4561 gagaacatga caggattaaa ggtcagagaa gttaacgtcc gtgtcacagg cgttgcagca
     4621 gaagaagagt aagaaagaat ataaagcaaa gtcagacggg actgatcgca gttccgtctt
     4681 tgtatcgcag aaaggaaccc taatgaaaag aagtgaggta agagaacata ttttcagaat
     4741 tttattttgt
//
LOCUS       DAOMUE010000154         4726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5046_length_4726_cov_2.911368, whole genome shotgun sequence.
ACCESSION   DAOMUE010000154 DAOMUE010000000
VERSION     DAOMUE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5046_length_4726_cov_2.911368"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PLC71_07375"
     CDS             <1..900
                     /locus_tag="PLC71_07375"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HRM02499.1"
                     /translation="DEKAVGEMFMGLPADTGMIISVGSGTLNDMAKYMSSRTKIPYTI
                     VCTAPSMDGYASSGAPLMNGGRKISYTATLPYAIIGDTDIMKNAPMKMILAGYGDIIG
                     KLTALADWKLSHEITGEYYCETIVKLVQKAINKVVDTRFDLAKRDEDAIFYLIEALIL
                     TGVAMGLIGVSRPASGAEHMLSHYWEMAVIATGKNPELHGIKVGIATPIITKIFDKMK
                     DTLPESVFKMAPSAKYTTQLLRDVNAPINPQEAGIDNDLFYRSILEGNTVRKRYSILD
                     YAVKHGNIEKIADEITRNYYMDI"
     gene            complement(1084..2214)
                     /locus_tag="PLC71_07380"
     CDS             complement(1084..2214)
                     /locus_tag="PLC71_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HRM02500.1"
                     /translation="MSETKTLQVMYDEKLVGTLAMASGHKVAFQYSEEWLEDGFSISP
                     FSLPLKNQVFVPQKDYFDGLFGVFADSLPDNWGRLLLNRLLRAHKQNPDRLTVLDRLE
                     IVGKSGMGAITYHPERTLEQTNGNTNLDELAEQCQKILNTEYSDKLDELYRLGGTSGG
                     ARPKIMTTIDEEEWIIKFPAHVDGKNAGKMEYDYSCCAKKSGITMSETRLFPSEQCDG
                     YFGTKRFDRRIDQTGKKRIHMLTAAALLELDFEQPSLDYHSLMKLTKILTRDNTEDIE
                     NMFRRMCFNVFAHNRDDHSKNFTYLYDEEKDSWRLSPAYDLTYSSTYYGEHTTTVDGN
                     GRNPGRKEILTVGITAGMKKEVCVDIMDQIEKCVRDMLGEYL"
     gene            complement(2201..2479)
                     /locus_tag="PLC71_07385"
     CDS             complement(2201..2479)
                     /locus_tag="PLC71_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005349716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM02501.1"
                     /translation="MEQLVWETAEELDQKLAQRVRKIRKRRSISQMKLASMSGVSYGS
                     IKRFETTGQISLISLTKIAMALDIADELRNIFTQVPYRNIQEVINERN"
     gene            2847..4568
                     /locus_tag="PLC71_07390"
     CDS             2847..4568
                     /locus_tag="PLC71_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM02502.1"
                     /translation="MSTFQKFIQYYKPYKKVFIFDLICAAFISLVDLAYPQILRSLTK
                     TLFLKDASMILKTLPIIGGILFICYIFQAFCKYYVSCQGHIMGAQMERDMRRKLFDHY
                     ENLSFSYYDQHNTGQMMSRLVSDLFDISEAAHHGPENLFISLVKIVGSFIFLFIIQWK
                     LAIILLAIVLLMIFFSAKQNRKMQATFLDNRRKIGDVNASLQDSLAGIRIVQSFANED
                     IEREKFRVGNEAFLDSKRDNYRAMGSFQSGNTFFQGMMYLVTLLAGGYFIALGQMSAA
                     DLAMYALYIGIFISPIQILVELTEMIQKGMSGFIRYQSVIDIEPEITDSVDAVNMKHP
                     DGDICYHDVSFSYEDNEIVLNHIDFTIKSGKSIALVGPSGGGKTTICSLLPRFYDITD
                     GLITIGGQNIKDIKLESLRSSIGIVQQDVYLFGGSVKENIAYGNPDATFDEIVEAAKK
                     ANIHDFIMTLPNGYDTFVGERGTRLSGGQKQRISIARVFLKNPPILILDEATSALDNE
                     SEQHIQKSLDALSENRTTITIAHRLSTIQNADEILVIDGQTIKERGTHEELIASGGLY
                     AKYYHMM"
BASE COUNT         1378 a          891 c          794 g         1663 t
ORIGIN      
        1 gacgaaaagg ctgtcggtga aatgtttatg ggacttcctg ctgatactgg aatgatcata
       61 tctgttggct ctggaacttt aaatgacatg gcgaagtata tgtcatcaag aacaaaaatt
      121 ccatacacta tcgtctgcac agctccatcc atggatggat atgcttcatc tggtgctcct
      181 ttaatgaatg gcggaagaaa aatttcttac actgcaacac ttccatatgc gatcattggt
      241 gataccgata ttatgaaaaa tgctcctatg aaaatgatcc tagctggata tggagatatt
      301 atcggaaaac ttacagctct ggccgattgg aaactttctc atgaaatcac cggcgaatat
      361 tattgtgaga cgatcgtaaa gcttgtacaa aaagcaatca ataaagtcgt tgatacccgc
      421 tttgatctcg ccaaacgtga cgaagatgcc atcttttatc tgattgaggc tctgatctta
      481 actggtgttg ccatgggatt gatcggagtt tcaagacctg catctggtgc agaacatatg
      541 ctgagtcact actgggaaat ggcagtaatt gcaactggca aaaatccaga gcttcacggc
      601 attaaagttg gaatcgccac accgatcatt accaaaatct ttgataagat gaaagacact
      661 cttcctgaat ctgttttcaa aatggctccc tctgcaaaat acactacaca actattaaga
      721 gatgtaaatg cacctataaa tcctcaggaa gcgggtattg acaatgattt gttttatcga
      781 agcatcttag aaggaaatac tgttcgcaaa cgatatagca ttcttgatta tgctgtcaag
      841 catggtaata ttgagaaaat tgctgatgag ataactagaa attattatat ggatatctaa
      901 gatcaactgg catttacaat aatttcttta gaactgttta tactttcttt agaattgttc
      961 atactttata tattgaatga aggaaggatt ttgctatact taagacatag tttttcatat
     1021 atatttttta atcctctctt ttcagaaaag taaccgctca ttttattgag cggtcttttt
     1081 atttcataaa tattctccta acatatctct tacacatttc tctatctgat ccataatatc
     1141 cacacatacc tcttttttca tccctgccgt aattccaact gttaaaattt cctttcttcc
     1201 aggattacgt ccattcccgt caactgtcgt ggtatgttct ccataatatg tgctgctata
     1261 agttagatca tatgctgggc ttagtcgcca actatctttc tcttcatcat ataaatatgt
     1321 gaaattcttt gaatgatcat ctcgattatg tgcaaaaacg ttaaaacaca ttctacgaaa
     1381 catattttca atatcctccg tattatctct tgttaatatc tttgtcaatt tcattaaact
     1441 atgataatca agacttggct gctcgaagtc caattccaaa agagctgcag cagttaacat
     1501 atgaattctt ttttttcctg tttggtctat tcttctatca aagcgtttcg ttccaaaata
     1561 accatcacac tgttctgatg gaaaaagtct tgtttcactc attgtaatcc cacttttttt
     1621 cgcacaacat gaataatcat attccatttt ccctgcattt tttccatcta catgagctgg
     1681 aaattttatg atccattctt cctcatcgat tgtcgtcatg atcttcggtc ttgcaccacc
     1741 actggttcct ccaaggcgat ataattcatc tagtttatct gaatattctg tattgagaat
     1801 tttctgacat tgttctgcca gttcatccag gtttgtattt ccatttgttt gttccaatgt
     1861 ccgctcagga tgataggtaa ttgctcccat tcctgacttt ccaacaatct ctaagcggtc
     1921 caaaacagtt aatctatctg gattctgttt atgtgcacgc aataatcgat tcaataataa
     1981 tcttccccag ttatctggca aactgtccgc aaatactccg aaaaggccat caaaataatc
     2041 cttttgcgga acaaacactt gattttttaa aggtaatgaa aatgggctga ttgaaaaacc
     2101 atcttctaac cattcctcac tatactggaa tgcaacttta tgccctgatg ccatagccag
     2161 tgttcccaca agcttttcat catacataac ttgtaacgtt ttagtttcgc tcattaataa
     2221 cctcctgaat attccgatat ggcacttgtg taaaaatatt tcgaagttca tctgcaatat
     2281 ctaatgccat tgctatctta gttaatgaaa ttaatgaaat ctgtcctgtt gtctcaaacc
     2341 ttttgatcga accataactt acaccactca tagaagctaa tttcatctga gaaatagaac
     2401 gccgttttct gatctttcgt actctttgtg caagtttctg atctaattct tctgctgttt
     2461 cccatactaa ttgttccatt tatttctcct cattttatag ataatatttt atccaaaatc
     2521 tcatattttt aataataata gataatattt tatctattat tattttatca tttatacttc
     2581 atttaaacaa gttttgtttg aatatattca atttctttag aactgtttat actttcttta
     2641 gaattgttca tactttatat attgaatgaa ggaaggattt tgctatactt aagacatagt
     2701 ttttcatata tattttttaa tcctctcttt tcagaaaagt aaccgctcat tttattgagc
     2761 ggtcttttta tttttcaaaa attggtgtat gatagtctct ggttgtttgt atacaatcaa
     2821 atttttcttt aaaggagtta tcaccaatga gtacttttca aaaattcatc caatattata
     2881 aaccctacaa aaaagtcttc atttttgatc tgatctgtgc agcatttatc agtctggttg
     2941 atcttgctta tccacagatc cttcgttcac ttacaaagac tttatttctt aaggatgctt
     3001 ctatgatcct taagactctt ccgatcatcg ggggaatttt atttatctgc tatatttttc
     3061 aggcattctg taaatattat gtcagctgtc agggacatat catgggagct cagatggaac
     3121 gtgatatgcg ccgtaaactg tttgaccatt atgaaaatct ttctttttcc tattatgatc
     3181 aacataacac cggacagatg atgagccgtc ttgtttctga tctgtttgat atttccgaag
     3241 ctgcccatca cggccctgag aatttattta tctctcttgt aaagatcgtt ggatctttta
     3301 ttttcctgtt tatcattcag tggaaattag cgattattct tcttgcgatc gttttactga
     3361 tgatcttctt cagtgcaaag caaaaccgta agatgcaggc taccttcctt gataaccgca
     3421 ggaagattgg tgatgtcaat gcttctttgc aagactccct tgctggaatc cgtattgtgc
     3481 agtcttttgc aaatgaagat attgaacgtg agaaatttcg tgttggaaat gaggcatttt
     3541 tagattccaa gcgtgataat tatcgtgcta tgggaagttt tcagagtgga aatacatttt
     3601 tccaaggaat gatgtatctt gtcactttac ttgctggtgg ttattttatc gctcttggcc
     3661 agatgagtgc tgctgacctt gctatgtatg cattgtatat tggaatcttt atcagcccga
     3721 ttcagattct tgtagaatta acagaaatga tccagaaagg tatgtctggt tttatccgtt
     3781 atcagtctgt tattgatatt gaacctgaga tcacagattc tgttgatgcc gttaatatga
     3841 aacatcctga tggggatatc tgttatcatg atgtctcatt tagttacgag gataatgaga
     3901 ttgtattaaa tcacattgat ttcacgatca aatcaggaaa atccattgca ctcgttggac
     3961 cttccggtgg tgggaaaaca acgatctgtt ctctgctgcc tcgattttat gatattacag
     4021 atggtttgat tacgatcggt ggtcagaata tcaaggatat aaaattagag agtcttagaa
     4081 gctccatcgg tattgttcaa caggatgttt atctatttgg cggcagtgtc aaagaaaata
     4141 ttgcttacgg aaaccctgat gcaacctttg atgaaattgt cgaagctgct aagaaagcca
     4201 atatccacga ttttattatg acattaccta atggctatga tactttcgtt ggagaacgtg
     4261 gtacaagatt atccggtggt cagaagcaaa gaatctctat tgcaagagtc ttcttaaaga
     4321 acccgccaat cttaatctta gatgaagcta ccagcgcatt agataatgaa agcgagcagc
     4381 atatccagaa gagtctggac gccttgtctg aaaatcgaac aacaatcacg atcgcccatc
     4441 gtttatcaac gatccagaat gcagatgaaa ttcttgttat cgatggacaa acgatcaaag
     4501 aacgaggaac acatgaagaa ctaattgctt ctggcggtct ttatgctaaa tattatcata
     4561 tgatgtaagt gtcaaaagta caacaacgtc gaaacctatt ttgtttagaa ctgtttataa
     4621 attctttaga attgttcata ctttgtatat tgaatgcctc ggggattttg gtatacttaa
     4681 gacatagttt ttcatatata ttttttaatc ctctcttttc agaaaa
//
LOCUS       DAOMUE010000155         4715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5066_length_4715_cov_1.980043, whole genome shotgun sequence.
ACCESSION   DAOMUE010000155 DAOMUE010000000
VERSION     DAOMUE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5066_length_4715_cov_1.980043"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PLC71_07395"
     CDS             complement(<1..768)
                     /locus_tag="PLC71_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HRM02503.1"
                     /translation="MKFKFGVDSFIWAEDFKEKDLWIIEKAKEIGFEVVDFAIANPYT
                     FPTEQVKAELARVGMDCVCTTTLTLETNPISPDGEIRKAAVAAMKKCVDICNTLGAPI
                     LGGVNYAGWGYLTKKPRTDQEWDWGVACMREVAEYAKETGDVTICVECVNRFETHFLN
                     IAEDAVKFCKDVGTGNVKVHLDCFHMIREEKSFSGAVKTCGKEYLGYVHVNENDRGIP
                     GTGLVPFKEFFQALDEIGYDGPLVIESFDPSFEELAGN"
     gene            complement(781..1233)
                     /gene="rpiB"
                     /locus_tag="PLC71_07400"
     CDS             complement(781..1233)
                     /gene="rpiB"
                     /locus_tag="PLC71_07400"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRM02504.1"
                     /translation="MKISLGCDEAAYQLKETIKQHLQEAGHEVIDNGVGEGEVELYPD
                     VAVRTCKKVTDGTCDRGILVCGTGIGMAMTANKVPGIRAAVCHDPFSTERSVLSNDAN
                     VMCMGARVIAPQLALYLLDIWMGLTFKNGPSTPKVERIMEYQKEFCGK"
     gene            complement(1281..2054)
                     /locus_tag="PLC71_07405"
     CDS             complement(1281..2054)
                     /locus_tag="PLC71_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM02505.1"
                     /translation="MEKKYLFFDIDGTLTDRSTGEIVPSAREALTRLEENGHFVAIAT
                     GRAHYKARKFTDANGFKNMVCCGGNGCVIDNELVENIPLDKDGCIKIIHQAEELGYGW
                     CVALDDTKDVYMTDDKFLRQVGKRKEPTRYIIDDMLEVEKADAIYKVYVAIPKEDEEK
                     LTTKELVGHLRFEPEYLMFQPDNKRGGIIKMMEHLNADIKDVVVFGDDYNDMDMFCPE
                     WFSIAMGNACQDLKDIANYVTDSNVDDGIKNACEHFGWI"
     gene            complement(2099..2761)
                     /locus_tag="PLC71_07410"
     CDS             complement(2099..2761)
                     /locus_tag="PLC71_07410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM02506.1"
                     /translation="MKTYKGIIFDLDGTLLDTIEDLRDSVNDVMKIYGWKEHDTKACK
                     QMVGNGFRKLIKRALPEDKQKNELFIDEAVNQFSKAYQKRYLNKTIPYEGILKLLESL
                     EEKGIKIAVNSNKRGDYTSALVNKYFSQFPWTAVYGERESEGIPKKPDPSAALEIADL
                     MKLSKEEILYIGDSKTDMQTGQNAGMDTIGVSWGFRGRKELEENHASYVVDHPEDILK
                     FI"
     gene            complement(2832..3746)
                     /locus_tag="PLC71_07415"
     CDS             complement(2832..3746)
                     /locus_tag="PLC71_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRM02507.1"
                     /translation="MAKLVDIQGRFPCEPDNKKPTLIKKWEYSTALYPPNNAFTSDNT
                     FTLVTTDTFMLGIYELGPGGVFAPLDIHPGDESYYILNGPVVQRSGNGQFAYLETGEG
                     LFMPEGAWHCCHNFSDQKTRILYFITPKAWDEHIPPAVIPTEEETKFYKGPNNDNLPD
                     MRGAIKDISRQGCTDDIGCWPVDPAEARKTGAVYAVRDFEKLNNVHGTTHPMLLRFIT
                     SNDYGHFGEMVLPAGGYGPRCSDPDVHKGDGALYCVDGPVTVNLVDAEESFVLEPEDT
                     FFLPAGTKYQLVNFENKPVKVVFAITEL"
     gene            complement(3784..>4715)
                     /locus_tag="PLC71_07420"
     CDS             complement(3784..>4715)
                     /locus_tag="PLC71_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HRM02508.1"
                     /translation="LGGYNIPSVEPYGSTFASTIFWIGKIGLGTFMVPVLGAYVAYSI
                     ADKPGICVGMFGGWMATDPWGLGYQSGFIGALIAGIIAGYLVNALKKIPLPNAIRSLL
                     PTLIIPVVGCAAICLLMYYVIGGPLGALTQALTKLLNDLGTGNLILLGIVQGCMLAFD
                     MGGPCNKVAYAFALACMETGNYAPMAANFVACCAPPLAVALAMLIAPKKFTDEDRSGI
                     PGCFAGALCMITEFAIPYAAKNIKYIPCFMVGSAAGAVISYLWGITIRAPHGGVFVVF
                     AMNKVLPFFITLLIAGAISAALIVLVTKPQEEA"
BASE COUNT         1250 a         1067 c          747 g         1651 t
ORIGIN      
        1 gtttcctgct aattcttcaa aacttgggtc aaaggattca ataacaagtg gtccatcgta
       61 tccgatctca tccaatgcct ggaagaattc tttaaatggt acaagtcctg ttcctggaat
      121 tcctcggtca ttttcattga catgtacata tccaaggtat tcttttccgc aagttttaac
      181 tgctcctgag aagctctttt cttcacggat catatggaag cagtcaagat gtactttgac
      241 atttcctgtt ccaacatctt tacagaactt cactgcatct tctgcaatat ttaagaagtg
      301 tgtttcgaaa cggttgacac attcgacaca gatggtcaca tctcctgtct cttttgcata
      361 ttcagcaact tctctcatac atgcaacacc ccagtcccat tcctggtcag ttcttggctt
      421 ctttgtaaga tatccccatc cagcatagtt tactccgcca aggattggtg ctcccaatgt
      481 attacagatg tcaacgcatt tcttcatcgc tgcaactgct gccttgcgaa tctctccatc
      541 cggtgaaatt gggtttgttt ccaaagttaa tgttgttgtg caaacacaat ccattccaac
      601 tcttgctaat tcagctttta cctgctccgt tggaaatgta tatggatttg cgattgcgaa
      661 gtctacaact tcaaatccta tttcttttgc tttttcaatg atccagagat ctttttcttt
      721 aaaatcttct gcccagataa agctgtctac tccaaattta aatttcataa caacaccctc
      781 ctattttcca caaaattctt tctgatattc catgattctt tctacttttg gtgtggatgg
      841 tccatttttg aatgtaagtc ccatccagat atctaataaa taaagtgcca actgtggtgc
      901 aatgactctt gctcccatac acatgacatt tgcatcatta ctcaaaaccg aacgttctgt
      961 tgaaaatgga tcatggcaaa ctgctgctcg gattcccggc actttattgg ctgtcattgc
     1021 cattccgatt cctgttccac atacaagaat tcctcgatca caagtaccgt ctgttacttt
     1081 cttacaggtt cttactgcaa catccgggta taattcaact tccccttctc ctactccatt
     1141 atcaatgact tcatgtcctg cttcctgaag gtgttgtttg attgtttctt ttaactgata
     1201 agctgcttcg tcacatccca atgatatttt catgatcttt tccctccaat atctaacgga
     1261 aattaccgta ataagttata tcaaatccat ccaaaatgct cacatgcatt tttaattcca
     1321 tcatctacat tgctgtctgt tacataattt gcgatatctt ttaaatcctg gcatgcattt
     1381 cccatcgcaa tggagaacca ttctgggcaa aacatatcca tgtcattgta gtcgtcccca
     1441 aagactacaa catctttgat atctgcattt aaatgttcca tcatttttat gattccgcca
     1501 cgtttattat ctggctggaa cattaaatat tctggttcaa atcttaaatg tccaacaagt
     1561 tcctttgttg tcagcttctc ttcatcttct tttggaattg ctacgtatac tttataaatg
     1621 gcatctgctt tttctacttc caacatgtca tcaatgatat aacgtgttgg ttcttttctt
     1681 tttccgacct gacgtaagaa tttatcatcc gtcatgtaca catctttcgt atcatctaat
     1741 gccacacacc atccataacc aagttcttct gcctgatgaa tgatcttaat gcatccgtct
     1801 ttatctaatg gaatattttc tactaactca ttatctatga cacagccatt tcctccacaa
     1861 cataccatgt tcttaaatcc attggcatct gtgaattttc tcgctttgta gtgagcgcgt
     1921 ccagttgcga tcgcaacgaa atgtccattt tcttctaatc ttgtcaatgc ttctcttgca
     1981 cttgggacga tctcgcctgt tgatctgtct gtcaaagttc catcaatgtc gaaaaaaagg
     2041 tattttttct ccatattctt atcctttctt ctgcacccac ttgtctgtct ttcttcgatc
     2101 agataaattt caagatatct tccggatgat caaccacata ggatgcatga ttttcttcca
     2161 gttcttttct tcctcgaaat ccccatgaaa caccgatggt atccatacct gcattctgcc
     2221 ctgtctgcat atctgttttt gaatctccaa tatataaaat ttcttctttt gaaagtttca
     2281 tcaaatctgc gatttctaat gctgcgcttg gatctggttt cttaggaatc ccttcacttt
     2341 cacgttctcc atacactgcg gtccatggga actgtgaaaa atatttattt accagcgcac
     2401 tggtataatc tcctcgttta ttggaattaa ctgcaatttt aattcctttt tcttctaagc
     2461 tctcaagcaa tttaagaatt ccttcataag gaattgtttt gtttaaatat ctcttctgat
     2521 acgcttttga aaattgattc actgcctcat caataaacaa ttcatttttt tgtttatcct
     2581 ctggcaacgc ccttttgatc aatttacgaa agccattccc taccatctgt ttgcatgctt
     2641 tcgtatcatg ttctttccat ccatagatct tcataacatc attgacgctg tctcttaaat
     2701 cttcgatcgt atctaataaa gttccatcta aatcaaaaat aattcctttg tatgttttca
     2761 tctttgtttt cctttctggc tttagttaat atgggatgat aaagctgcct ttatcatccc
     2821 atattataca attacagctc agtaatggca aagactactt ttactggttt attctcaaaa
     2881 tttactaact gatattttgt tcctgctggt aagaagaatg tatcttctgg ttccagtaca
     2941 aaactttctt cagcgtcaac taggtttaca gtcactggtc catctacgca atataatgct
     3001 ccgtcacctt tgtgaacatc tggatctgaa catcttggtc cgtatcctcc tgctggaagt
     3061 accatttctc caaagtgtcc gtaatcatta cttgtaatga aacgaagtaa cattggatga
     3121 gttgttccat gaacattatt taacttttcg aagtcacgta ctgcgtatac agctcctgtt
     3181 tttctagctt ctgctggatc tactggccag catccgatgt catctgtaca tccctgacga
     3241 gagatatctt tgatcgctcc tctcatatct ggcagattat cattgtttgg tcctttgtag
     3301 aattttgttt cttcttctgt tggaatgact gctggtggaa tatgctcatc ccatgctttt
     3361 ggtgtgatga aataaaggat tcttgttttc tggtctgaga agttatgaca gcagtgccaa
     3421 gcaccttctg gcatgaataa tccttctcca gtttccagat aagcaaactg tccgtttcca
     3481 cttctctgta ctacaggtcc gtttaggatg tagtaagatt catcaccagg atggatatct
     3541 aatggtgcga atactcctcc aggccctaac tcgtaaattc ctaacataaa tgtatcagtt
     3601 gttactaatg taaaagtatt gtcagaagta aatgcattgt ttggtggata taatgctgtt
     3661 gaatactccc attttttgat caatgtaggt ttcttgttat caggttcaca aggaaaacgt
     3721 ccctgaatat caactaattt tgccatttta actctcctgt ctgttctttc tattaactgt
     3781 tttttatgct tcttcctgag gttttgtaac taatacaatt aaagctgctg aaattgctcc
     3841 ggcaattaat aatgtgatga agaatggaag tactttattc atagcgaata ctacgaatac
     3901 accaccatga ggtgcacgga tcgtaattcc ccataagtaa gagattactg ctccagctgc
     3961 tgatccaacc atgaaacatg gaatatattt aatatttttg gcagcatatg ggatcgcaaa
     4021 ttctgtgatc atacataatg ctccagcgaa acatcctggg attccggatc tatcttcatc
     4081 tgtaaatttc ttaggtgcga tcagcattgc taatgccact gctaatggag gtgcacagca
     4141 agctacgaag tttgcggcca ttggtgcata gtttcctgtt tccatacaag ctaatgcaaa
     4201 agcataagca actttgttac atggaccacc catatcaaag gcaagcatac atccttgtac
     4261 gattccgaga aggattaagt ttccagttcc taagtcattt aataattttg ttaatgcctg
     4321 tgttaatgct cctaaaggtc ctccaataac atagtacatt aataaacaga tagctgcaca
     4381 tccgacaact ggaatgatca atgtaggaag taatgatctt attgcatttg gtaatggaat
     4441 tttctttaat gcgtttacta aatatccagc aataatacct gcgatcagtg ctccgataaa
     4501 tcctgactgg tatccaagcc cccaagggtc cgtagccatc cagccaccga acatacctac
     4561 acagatacct ggtttatcag cgattgaata cgctacatat gcacctaata ctggtaccat
     4621 gaatgttcct aaaccaattt taccaatcca gaagattgtg gatgcgaatg tggaaccata
     4681 aggttctacg ctagggatat tatatcctcc taata
//
LOCUS       DAOMUE010000156         4711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5076_length_4711_cov_1.970361, whole genome shotgun sequence.
ACCESSION   DAOMUE010000156 DAOMUE010000000
VERSION     DAOMUE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5076_length_4711_cov_1.970361"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="PLC71_07425"
     CDS             complement(<1..1184)
                     /locus_tag="PLC71_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HRM02509.1"
                     /translation="MRTMKRVAAVALSVVVLMTSGVSAKAAVTDVQPAKSSSEAVLKW
                     AKKVGEPFDEKTYANNTVSKIKLVGNYLYVADDAKHRIMKIDKKDGTILKEQKYCDES
                     YIQGYVSSIGYGDNKVYVGYDNGRVQAFDENTLESLWITDENKNAINADFVFTNGKLY
                     FGTTAKTTDWYDNGAPFSYYVVTTSDDDKTKPDEEKTMKKVVESKTGKFYLKKGVVLG
                     KYIVISDTAGTLTMIDTTNDKVTDTKDIGEAFSGNITYDEMTGTIYFVAGTNDLYGIK
                     VSADGKLKDEKKVRLYETGYSSTTPEVYNGKVYVSGSKGKAFQDKGYMAVADVSSDKY
                     KVNYTVELSNYSQCEPTIVKTGENSVRVYFTINDEPGGIYAIEDSKDATSATLETVYE
                     PTG"
     gene            complement(1205..1837)
                     /locus_tag="PLC71_07430"
     CDS             complement(1205..1837)
                     /locus_tag="PLC71_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRM02510.1"
                     /translation="MLNAVIIFMVCISCIPYYFKYEKKKPQTRESVVLAVMIALTVAS
                     RMLFAFSPGFKPVSAMVIICGMAFGRESGFLCGSLSAVVSNFFFGQGPWTPFQMLAWG
                     MIGWISGILNQRKWLENSKILLTIFGILSGISYSFVMDIWTLLAAGDGFQWMRYVAVL
                     GTSVPVTIEYCVSNVIFLWILTPVFVKKLNRVKYKYGFFKDEEVRKLINQ"
     assembly_gap    1920..1987
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1988..2841)
                     /locus_tag="PLC71_07435"
     CDS             complement(<1988..2841)
                     /locus_tag="PLC71_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM02511.1"
                     /translation="MEMSDECVILDDGKVIEQGNPKEVARHLQKIHHPLEQSLPQILR
                     LEEKFQVELTFSMEEAREQIRKKEYQLIKKTHFERKTVLSISHLYAGYEKGKDVLSNL
                     SVEVKEGEIFAAVGANGSGKSTLLSCMVKQMKFDGKLKCKKKIVYMPQDPTLLFVKDQ
                     LFEDLLEMGKEKEVKIDKLLEMADLMREKKSHPYDLSGGQQQMAALIKALLADPEILL
                     LDEPTKGMDREHSRKFGELLRKLTDQGKTIFIVSHDLEFCAEYADRVGMMFDGKIEGI
                     DEPSKFFA"
     assembly_gap    2881..2931
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2932..3471)
                     /locus_tag="PLC71_07440"
     CDS             complement(<2932..3471)
                     /locus_tag="PLC71_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM02512.1"
                     /translation="MYEINNLTFRYMLSDRNSLEQVSLKIKKGKITLIAGPSGSGKTT
                     LLRHLKKELLPKGKRSGKVLYDEQEIEQLKDLQSVKEVGYLFQNPSAQMVMDTVWHEI
                     AFGLENLGMPYEQMKRTVAEIVNYFDLQKIYHEDTDKLSGGQKQLVNLAAVMAMHPKV
                     LILDEPTAQLDPAARKDFLV"
     gene            complement(3458..4294)
                     /locus_tag="PLC71_07445"
     CDS             complement(3458..4294)
                     /locus_tag="PLC71_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRM02513.1"
                     /translation="MIKALKRAHPFVVFTYFAGMFLLAPFSRHPWMTGVQMICLCLLY
                     FYHNRSLKKFFPLIALILIVAVTNPLFVQRGRTILYADTHVRITKEALLYGIHYGCVL
                     VNLLLVFSIFNQYIKKEQWIYLSGRFFPKLGVVTSMAFGLIPRYQNHAKKILNTRKSL
                     KSEQAVQRVMNTVSMETTWAFESSMDQLDSMGARGYGIGKRTHFHLFRWERNDWIHIV
                     EIVILTILNLWAYIHYYSRFFFYPMIIMPAFQMRDLLWMLMMAFQFLLPCMWKENFDV
                     RN"
     gene            complement(4295..>4711)
                     /locus_tag="PLC71_07450"
     CDS             complement(4295..>4711)
                     /locus_tag="PLC71_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HRM02514.1"
                     /translation="TTTASKNESTVQTAENQTTEAKQEQSCEFLISCKTVLSNKSALQ
                     SNYQVPAGGKIYEKKMEFEEGDTVMDVLKRTGVDIDVSKGYVAGIDGLYEFDCGKNSG
                     WMYRVNGKFPNYMAGKCKLHDGDKVEWLYTCVRGDL"
BASE COUNT         1306 a         1043 c          634 g         1609 t
ORIGIN      
        1 ccagttggtt cataaactgt ttccaatgtt gctgatgttg catcttttga atcttcaatt
       61 gcataaattc cacctggttc atcattaatt gtgaaatata cacgcacaga attttcccct
      121 gtttttacaa ttgttggttc acactgagaa taattagata attctactgt ataatttact
      181 ttatacttat cagaacttac atctgcaact gccatatatc ctttatcttg aaatgctttt
      241 ccttttgatc cagatacata aacttttcca ttatatactt ctggtgtagt tgaggaatat
      301 cctgtttcat ataatctgac ttttttctca tcttttaatt ttccatcagc tgatactttg
      361 ataccgtaca gatcatttgt tcctgctaca aaataaattg ttcctgtcat ttcatcataa
      421 gtgatattac cagaaaaagc ttctccaata tcctttgtat ctgtaacttt atcatttgtt
      481 gtatcgatca ttgttaaagt tcctgctgta tcagaaataa caatgtattt tccaagaact
      541 acaccttttt tcaaataaaa ttttcctgtt ttagattcta ctactttttt cattgttttt
      601 tcttcatcag gttttgtttt atcatcatct gatgtcgtta caacataata agaaaatggt
      661 gcaccattat cataccaatc tgttgtcttg gctgttgtcc caaaatataa ttttccattt
      721 gtaaatacga aatcagcgtt aattgcattc ttattttcat ctgtaatcca taaagattct
      781 aatgtgttct catcaaatgc ctgtacacga ccattgtcgt accctacata aaccttatta
      841 tctccatatc caatactgct gacatatccc tgaatataag attcatcaca atatttctgt
      901 tcttttaaaa ttgttccatc tttcttatca attttcatga tacgatgttt tgcatcatct
      961 gctacataaa ggtagtttcc aaccaacttg atcttagata ctgtgttatt tgcatatgtt
     1021 ttctcatcaa atggttctcc aactttcttt gcccacttaa gcaccgcctc actactactc
     1081 ttcgctggct gcacatccgt cactgctgct tttgcactaa cccctgatgt catcagcacg
     1141 acaacagaca atgccactgc cgctacacgt ttcatcgttc tcataaattc ctccttgtta
     1201 tgtctcattg attgattagt ttcctgactt cttcatcttt gaagaaacca tatttatatt
     1261 taaccctgtt tagtttcttt acaaaaacag gtgttaaaat ccataaaaat atcacattcg
     1321 atacgcagta ttcaatcgtc actggaaccg aagttcctaa tactgccaca taccgcatcc
     1381 actgaaatcc atctcctgct gccagaagtg tccagatatc catcacaaaa gaatatgaaa
     1441 ttcctgaaag aatcccaaaa atcgttaaca gtatcttact gttttccaac cattttctct
     1501 gatttaaaat tcctgagatc catccgatca ttccccatgc aagcatctga aatggagtcc
     1561 atggtccttg tccaaaaaag aagtttgata ccacggcact taaagatcca cacaaaaaac
     1621 cggattctct tccaaatgcc ataccacaga tgatcaccat tgcactaact ggtttaaatc
     1681 ctggtgaaaa tgcaaataac attctggatg caaccgtcaa tgcgatcatc accgcaagca
     1741 cgacactttc tcttgtctgc ggtttcttct tttcatattt aaaatagtat ggaatacatg
     1801 atatacacac cataaaaata atcaccgcat ttaacatacg cttaccacct cctgtggcag
     1861 aatgatcact ttctcaaaat ctctcgtaat ctttgcacac tctgtcgtat agaaataatn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnntgt gcaaaaaact tggatggctc atcaattccc tcaattttcc catcaaacat
     2041 cattccaacg cggtcagcat actcagcaca aaattccaga tcatgcgata cgataaatat
     2101 cgtttttccc tgatctgtaa gtttgcgtaa aagttctcca aacttcctgc tgtgttcccg
     2161 atccattcct ttggtcggtt catctaacag caagatttct ggatctgcaa gtaatgcttt
     2221 gatcaacgct gccatctgct gctgtcctcc acttagatca tatggatgtg acttcttttc
     2281 tctcattaga tccgccattt ccaataattt atcgattttt acttcttttt cttttcccat
     2341 ctccaaaagg tcttcaaaca actgatcttt tacaaacaat agtgttggat cttgtggcat
     2401 atagacgatt tttttcttgc attttaattt tccatcaaat ttcatctgct ttaccataca
     2461 agaaagaagc gttgattttc cacttccatt tgctccgaca gctgcaaaga tttcaccttc
     2521 tttaacttcg actgacagat tcgaaagaac atcttttcct ttctcatatc ctgcatataa
     2581 atgtgaaatt gaaagaactg tttttctttc aaaatgtgtt ttctttatca actgatattc
     2641 ttttttcctg atctgttctc ttgcttcttc catcgaaaag gtcagttcta cctgaaattt
     2701 ctcttctagc cgaagaatct gtggcagaga ctgttccaat ggatgatgta ttttttgaag
     2761 atgtcttgca acctctttcg gatttccctg ttcaataact tttccatcat ctaagatcac
     2821 acattcatct gacatttcca tcacatcttc caagttatgt gatgtcaaaa taatcgttaa
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacaagaaa
     2941 atctttcctt gccgctggat ctaactgtgc ggtcggttca tctaagatca atacttttgg
     3001 atgcattgcc ataactgctg ccagattcac caattgtttt tgtccgccag atagcttgtc
     3061 tgtatcttca tggtatattt tctgcagatc aaaataatta acgatctcgg ctactgttcg
     3121 cttcatctgt tcataaggca ttccgagatt ctcaagtcca aatgcgatct catgccatac
     3181 ggtatccatt accatctggg ctgatggatt ctgaaataaa tacccaactt cttttacaga
     3241 ctgcaagtct ttcaattgtt ctatttcctg ctcatcatat aaaacttttc cagatctttt
     3301 acctttgggc agcagttctt tttttaaatg tcttaacaac gtcgtctttc cactgccgct
     3361 tggccctgcg atcagagtga tctttccttt tttaattttt aatgatactt gctctagaga
     3421 atttctatcc gataacatat atcgaaatgt taaattatta atttcgtaca tcaaaattct
     3481 ccttccacat gcatggtaat aaaaactgaa atgccatcat aagcatccac aacagatctc
     3541 tcatctgaaa tgctggcata atgatcatcg gataaaagaa aaatctggaa taatagtgga
     3601 tgtatgccca gagatttaat atcgtcaaaa tcacaatctc cacgatatga atccagtcat
     3661 ttctctccca cctaaataaa tgaaaatgcg ttcgtttccc aattccataa cctcttgcac
     3721 ccatcgaatc caactgatcc atcgaagact caaatgccca tgtcgtttcc attgacactg
     3781 tattcatgac acgctgtaca gcctgttctg attttaagga ttttcgtgtg tttaaaatct
     3841 tctttgcatg gttttgatat cttgggatca atccaaatgc catggatgtt acgacaccaa
     3901 gtttagggaa gaatctgcct gataaataga tccactgctc tttctttata tattggttaa
     3961 agattgaaaa gaccagtaaa agatttacta atacacatcc gtaatgaatt ccataaagca
     4021 atgcttcttt cgtgatcctt acgtgtgtgt ctgcatatag gattgttctt cctctttgta
     4081 caaataatgg attcgttaca gccacgatca agatcaaagc aatcaacgga aaaaactttt
     4141 tcaaactccg attatgataa aagtacaaca gacataaaca gatcatctgc actcctgtca
     4201 tccatggatg tctgctaaat ggtgccagca aaaacattcc tgcaaaataa gtaaatacaa
     4261 caaacggatg ggctcttttt aatgccttga tcatctacag atctcctctg acacaggtat
     4321 acagccattc cactttatcc ccatcatgaa gcttacattt ccctgccata tagtttggaa
     4381 acttaccatt gacacggtac atccatccac tgttttttcc acaatcaaat tcgtataaac
     4441 cgtcaattcc tgctacatat cctttagaaa catcaatatc tactcctgtt cttttgagaa
     4501 catccatcac ggtatcgcct tcttcaaact ccatcttctt ctcataaatt ttcccgccag
     4561 ctggaacttg gtaattggat tgcaatgcac ttttattaga aagaactgtt ttacagctga
     4621 tcagaaattc acaagattgt tcttgctttg cttctgttgt ctgattctct gccgtctgta
     4681 ctgttgattc atttttactg gctgttgttg t
//
LOCUS       DAOMUE010000157         4640 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5184_length_4640_cov_2.876772, whole genome shotgun sequence.
ACCESSION   DAOMUE010000157 DAOMUE010000000
VERSION     DAOMUE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5184_length_4640_cov_2.876772"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4640
                     /locus_tag="PLC71_07455"
     CDS             <1..>4640
                     /locus_tag="PLC71_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HRM02515.1"
                     /translation="VTVNKNTSQYLKEQQYEGIIQVVKTFGEENVPEAQAVFEVYDSK
                     NNLKQTITTNDKGIAETDLLPYGAYRIHQTKTTDGYDMVPDKWVSIDGSKMIYKVESN
                     DPKQHAGIMLTKVTRISDKETGTYTKKEEYKAEFQIIDQSTKKVIETLITDENGAAQS
                     GKLDPGIYTVHQTKGSDNYKIADDFDVTIKDGDKKFKKFELDNYYNGDKIRVRKTMVK
                     NGKSKPEPGAEFVVLDESMVKDFKDQTLATSQDRLHYIEKLKKDNRDAILGTLVTDSE
                     GKAAMLLKDFVKKTGFIVVQTRGVEGYDLALPQYSTEMKASIEDEVKVYEFVLKDDYT
                     KSAKISIKKQMSVNKDKYVPESGAEFQIIDPHGEVVDTLITDEKGEATSISLAIGVYT
                     LHQIKGDPKHEMMEDEDVVLIKSDVHKTVTFGPYKDDENKIRIELVKRSSETKKLLND
                     AVYEIYDEDQDIVATLTTGTSGDGTAECYLPYGKYTIKETAAPAGYNMDDAEEKSFEL
                     SGESTSIKITYDNAGNGSCRFDQMDTPVYGEITFSKTGDILTDYDKDFQSFIYDNDQI
                     AGAVYGLYADENIKKDDGTLVWKKDELIDQKTTTKEKEIHFTRKDMDGKETTNFYLGK
                     YYIKEIKSPTGYIKDQEKHEVELTWDTTAGSINDIRDDDKVSDKEDPFGNEDNNVSTG
                     IYVLEKGEKLNQKIKDAESVTFTWKSAPEGAVTTDVSQNKDGSIVLWNDDGDCYISSQ
                     RAGQVIYMNAISSKMFKNCRNLTEINFKNIDTSAVVDMSQMFYAMDSIKTLDLSSFNT
                     SNVEDVSQMFYGNPVLKTTYVMDQILKIEEDKFIEEQPLKIVAMPKNRFLKGDKFKAE
                     DFSWRIFYDDDGAEDVEVTDKDVAFTPTYAEKSGKYEINISFNNQGSYKDLPEKTIKT
                     TVEVLDPEEEDIALKVAKKASIHVKANDTLQKYGIHLIKTDQDGNKLAGAIFALKAKT
                     DIVNSKGENIFKKGDTIATTTSQDDQFEYLEFIGLPTDIYAKDGTDSDMYEVEEIYAP
                     DGYEKSDKTLTFKGKVINDTKENLIHDVKAENNTDNDTTTYVHDSDTLVNQKLDYIAL
                     KKIWDDHDNAAKIRPDVVTIKATHKTTKEVKVYTLTKDNNWTMFTDIKKDDREKWEFH
                     EDVPEGYTLTSTHWNDAMYVISFTNFHENPEYVDINVQKVWEDGDNGDNLRPISIKAQ
                     LYRNGEKYKNAITLDETNNWSDDVTYHCLDKYDKAGEPFEYEVKEEVYDKLTGNAKTG
                     YVDTYETNETIDDDGTTTKNIMITNTHTPDTTKKKVKKVWNDHGNKDGIRPKSVLVNL
                     LADGKVEETLMLSAKNNWKASSKVLPVKVNGKKVNYTWKEVEEGLITGESEIGYRPTY
                     DTDENDQDLTVITNTHDRTGPKGSITITKALDPGNLNMDVGDPTFIFTLTGTDVYGKK
                     HSYEKKVKFTKAEVEKQMKDHPGDLIELSVTFDDLEYGTYTCNEGGMIKYFKLLNITS
                     NRSNATIDQEKETVTFDIGPTGTTAKAQLTGGAKFMNQMIQGSVKLIKKDSKG"
BASE COUNT         1744 a          718 c         1086 g         1092 t
ORIGIN      
        1 gtcacggtca ataaaaatac atcccagtat ctgaaggaac agcaatatga aggaataata
       61 caagttgtaa agacgtttgg cgaggaaaat gttccagaag cacaggcagt ttttgaggtt
      121 tatgattcca agaataactt aaaacagacg atcacaacga atgacaaagg gatcgcagaa
      181 acagatttac ttccttatgg agcttaccgt attcatcaga cgaaaacgac cgatggatat
      241 gatatggtac cggacaaatg ggtaagcatt gatggatcta aaatgatcta taaggttgaa
      301 tcaaacgatc caaagcagca tgcagggatc atgttgacaa aggttacaag gatcagtgat
      361 aaggaaactg gaacttatac aaaaaaagaa gaatataagg cagaattcca gatcatcgat
      421 cagtcgacaa agaaagtcat agaaacattg atcacggatg aaaatggtgc agctcaatct
      481 ggaaagttag atcctggcat ctacacggtt catcagacaa aaggaagtga taattataag
      541 atcgctgatg attttgatgt aacgatcaaa gacggcgata agaaatttaa aaaatttgag
      601 ctggataatt attataatgg agacaagatc cgggtcagaa aaacgatggt caagaatgga
      661 aagtctaaac cagaaccagg ggctgagttt gttgtactgg atgaatctat ggtgaaagat
      721 ttcaaggatc agacattggc aacgtcacag gacaggcttc attacattga aaaactgaaa
      781 aaagataata gagatgcgat tcttggaaca ttagtcacag attccgaagg aaaggcagcg
      841 atgctcctaa aagatttcgt aaagaagaca gggtttatag tcgtgcagac cagaggtgtc
      901 gaaggctatg accttgcgct accgcagtat tccacagaaa tgaaagcatc gatcgaggat
      961 gaagtcaaag tgtatgaatt cgttttaaaa gacgattata caaaatccgc aaagatttcg
     1021 atcaaaaagc aaatgtccgt aaataaagat aagtatgtac cggaatctgg ggcggaattt
     1081 cagatcattg atccgcatgg agaagtggtc gatactctga taactgatga aaaaggagaa
     1141 gcaacttcaa tctcgttagc gatcggtgtt tatacattgc atcagatcaa gggtgatcca
     1201 aagcatgaaa tgatggaaga tgaagatgtg gttttgatca agtctgatgt ccataagaca
     1261 gtcacgtttg gtccgtataa agatgatgaa aataagatca ggatcgaact agtcaaacgt
     1321 tcatcagaga caaagaagct gttaaatgat gcagtctatg aaatctatga tgaagatcag
     1381 gatattgtgg caactttgac tacaggaacg agtggagatg gaactgcaga gtgctatctt
     1441 ccatatggaa aatatacgat caaggaaact gctgcgccag caggctacaa catggatgac
     1501 gcagaagaaa aatcttttga gttaagcggg gaatccacaa gcatcaagat cacgtacgat
     1561 aatgctggaa atggaagctg caggtttgac cagatggata cgccagtcta tggagaaatt
     1621 acgttcagta agacgggaga tatcttaaca gattatgaca aggattttca gagttttatc
     1681 tatgacaacg atcagatcgc aggagcagtc tatgggctgt atgcggatga gaatatcaag
     1741 aaagatgacg gaacgcttgt ctggaagaag gacgaactga tcgatcaaaa gacaacgaca
     1801 aaagagaagg agatccattt tacaagaaag gatatggatg gaaaagaaac aactaacttc
     1861 tatttaggaa agtattacat aaaagagatc aagtctccga caggctatat caaagatcag
     1921 gaaaaacatg aagtagaact gacatgggat acaactgcag gaagtatcaa tgatattcgt
     1981 gatgatgata aagtatcaga caaggaagat ccgtttggaa atgaagacaa taatgtaagt
     2041 actggtattt atgtgttgga aaaaggcgaa aaattgaacc agaagatcaa ggatgcggaa
     2101 tctgtgactt tcacgtggaa gagtgcacca gaaggagctg tgacaacgga tgtttctcag
     2161 aataaagatg gcagtattgt gctttggaac gatgatggag attgttatat ctcaagtcaa
     2221 agagccggac aggtcatcta catgaatgca atatcatcta aaatgtttaa aaattgcagg
     2281 aatctcacag agatcaattt taaaaatatt gatacatccg cagtcgtgga tatgagtcag
     2341 atgttttatg ccatggattc catcaaaaca ctcgatcttt catctttcaa cacctccaat
     2401 gtagaagatg tcagccagat gttctatgga aatccagttc ttaagacaac atatgtcatg
     2461 gatcagatct taaagatcga agaagataag tttatcgagg aacagccgtt aaagatcgtt
     2521 gccatgccaa agaacaggtt cttaaaaggt gataaattca aggcagagga ctttagctgg
     2581 agaatctttt atgatgacga tggagcagaa gatgtggaag tgacggataa agatgtggca
     2641 tttacaccga catatgcaga aaaatcagga aaatacgaga tcaatatcag ttttaacaac
     2701 caaggatcgt ataaagatct gccagagaaa acgatcaaga ctacggtaga ggttctggat
     2761 ccagaagagg aagatattgc cttaaaggtt gcaaagaaag caagtattca tgtcaaagct
     2821 aatgatactc tgcaaaaata cggcatccat ctgatcaaga ccgatcaaga cggaaacaag
     2881 ctggcgggag ccatatttgc cttaaaagcg aaaacagata tcgttaatag caaaggagaa
     2941 aatatcttta agaagggtga tacgatcgcc accactacat cacaggatga tcagtttgaa
     3001 tatctggaat ttattggatt gccgaccgat atctatgcaa aggatggaac agacagtgat
     3061 atgtatgagg tagaagaaat ctatgcacca gatggatacg agaaatcgga caagacatta
     3121 acatttaagg gtaaagtgat caatgatacg aaagaaaatc ttattcatga tgtaaaagcc
     3181 gagaataata cggataatga tacaacaaca tatgtgcatg attcagatac tttagtcaac
     3241 cagaaactgg attatattgc attgaagaag atatgggatg atcatgacaa tgcagcaaag
     3301 atccgtccag atgttgttac gatcaaggca acacataaga caacaaaaga agtaaaggtg
     3361 tatacactga caaaagataa taactggaca atgtttacgg atatcaagaa ggatgatcgt
     3421 gaaaagtggg agtttcatga agatgttccc gaaggttata ctttgacgag tacacactgg
     3481 aatgatgcga tgtatgtgat ttcgtttacg aacttccatg aaaatccaga atatgtcgat
     3541 atcaatgtac aaaaggtttg ggaagatggt gacaatggag ataatctgag gccgatatcg
     3601 atcaaagctc aattatatag aaatggtgag aaatacaaga atgctattac attagacgaa
     3661 acaaataact ggtcagatga tgtaacgtat cattgcctcg ataagtatga caaagctggc
     3721 gaaccatttg agtatgaagt gaaagaagaa gtctatgata agctgacagg taatgcaaaa
     3781 acaggatatg tcgatactta tgagacaaat gagacaatag atgatgatgg tacaacaacg
     3841 aagaatatca tgatcacgaa tacgcataca ccagatacga cgaagaaaaa ggttaaaaag
     3901 gtatggaatg atcatgggaa taaagatggc atacgcccaa agtctgtctt agttaatctg
     3961 ttggctgatg gtaaagtgga agaaacattg atgctcagtg cgaagaataa ctggaaagcg
     4021 tccagtaagg tacttcctgt gaaagtcaat ggtaaaaaag tcaattatac atggaaagaa
     4081 gtcgaagaag gattgatcac gggagaatct gagatcggat acagaccaac ctatgatacg
     4141 gatgaaaatg atcaggatct tacagtgatc acaaacacac atgaccgtac agggccgaaa
     4201 ggaagcatta cgatcacaaa agcattggac ccaggtaatc tgaatatgga tgttggggat
     4261 ccgactttta tatttacact gactggaacc gatgtctatg gaaagaaaca cagttatgag
     4321 aagaaagtca aattcacaaa ggcagaggtt gaaaaacaga tgaaagatca tccgggagat
     4381 ctgatcgaac tgtctgtgac gtttgatgat ctggaatatg gtacttatac gtgtaatgaa
     4441 ggaggtatga tcaagtattt caaattactc aatattacga gcaacagaag caatgcaacg
     4501 atcgaccagg agaaagaaac tgtgacgttc gatattggac caactggaac gacagctaaa
     4561 gcacaattga caggtggagc gaagtttatg aatcagatga tccaaggatc tgtaaaactt
     4621 atcaagaaag attctaaggg
//
LOCUS       DAOMUE010000158         4637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5190_length_4637_cov_2.057180, whole genome shotgun sequence.
ACCESSION   DAOMUE010000158 DAOMUE010000000
VERSION     DAOMUE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5190_length_4637_cov_2.057180"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      196..278
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            395..1129
                     /gene="pflA"
                     /locus_tag="PLC71_07460"
     CDS             395..1129
                     /gene="pflA"
                     /locus_tag="PLC71_07460"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HRM02516.1"
                     /translation="MTVGHVHSIESMGLVDGPGIRTVIFLQGCALRCRFCHNPDTWEL
                     SGGTEYTPEKLVAKIRRFKPYFKEDGGVTFSGGEPLLQPDFLKETLKLCKNEGIHTCI
                     DTAGYGLSDYDEILNHTDLVLLDLKHIHKTDYEKMTGRSMDRFEEFLNALKKHQTKIW
                     IRHVVVPGITDSEDHMAQLKAYIQKIPNVEKVELLPYHLLGTNKYKVMDIPYSLEGVP
                     AMDKKKTEMLQQTYFDHVYFTEEKSC"
     gene            1123..3316
                     /gene="pflB"
                     /locus_tag="PLC71_07465"
                     /pseudo
     CDS             1123..3316
                     /gene="pflB"
                     /locus_tag="PLC71_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            complement(3371..>3649)
                     /locus_tag="PLC71_07470"
     CDS             complement(3371..>3649)
                     /locus_tag="PLC71_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02517.1"
                     /translation="TVRQTDAKQGLSEEVIAELNKKLYLIAETIGTQQMVEITYFVPD
                     AKKDGGAYITCSGCVKKIDKYKHTVVMTDQTIIPIEQISDIEGEMFEE"
     assembly_gap    3650..3659
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3841..>4637)
                     /locus_tag="PLC71_07475"
     CDS             complement(3841..>4637)
                     /locus_tag="PLC71_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HRM02518.1"
                     /translation="HGLFSMGDVARCSVGKPNEFHNEELLYKMFGINAELLIDHAWGY
                     EPCTMEQVKAYKPETNSVCSGQVLHCPYDFDKAKLVVKEMTDLMTLDLVDKRLVTDQI
                     VLTVGYDIENLTDPDRYRKYKGSVTIDRYGRKVPKHAHGTTNLKRKTSSTMLITDAVM
                     ELYDRIVDKNLLIRRINITANKLVDESFSKEEETYEQLDLFTDYTVKEQEQAEEEAVL
                     EREKRMQQTMLTIKKKFGKNAILKGMNLQEGATAKDRNEQIGGHKA"
BASE COUNT         1375 a          904 c          887 g         1461 t
ORIGIN      
        1 agattcttca tatagttcat tgtgatgata ccaactccat cttctacaac gaactgcaat
       61 ttctttagct tcataggcat atcctccttt aagttgtttt gataactata ttgtatcatt
      121 ttttgtacaa tttgtgaagt ttttcgacaa aattaagggg ttgacgaatc tcattttcta
      181 tagtatctta tagccataag gtcatataac tgacggagca cgtggaatta accacatgga
      241 agtatgatca ttaatcgccg accgtctggg caatctgtct ggacgtgcgg cgttttttta
      301 ttctattttt acaacgcttt atcgttcaga tattaatcct gttaactttt tacgtacttg
      361 tcctaagtac gtatcatcca taaggaggaa tactatgaca gttggtcatg tccattccat
      421 cgaatcgatg ggtctggttg atggaccagg gatacgaacc gttatctttt tacaaggatg
      481 tgctctaaga tgtcgtttct gccataatcc tgatacttgg gaattatctg gcgggaccga
      541 atacacaccg gaaaaacttg ttgcaaagat ccgacgattt aaaccttatt ttaaagaaga
      601 tggtggtgtt accttttctg gtggagaacc tttattacaa cctgattttt taaaagagac
      661 tttaaaacta tgcaagaatg aaggcattca cacttgcatc gacactgcag gttatggact
      721 ttctgattat gatgagattt taaaccatac agatcttgtt cttcttgatc tgaaacacat
      781 ccataaaacg gattatgaaa aaatgacagg aagatcaatg gatcgttttg aagaattttt
      841 gaatgcttta aaaaaacatc agactaagat atggattcgc catgtcgttg ttccaggaat
      901 tactgacagc gaagatcata tggctcagtt aaaagcttat attcaaaaga ttcctaatgt
      961 agagaaagtt gagctgcttc cttatcattt acttggtacc aacaaatata aagttatgga
     1021 tattccttat tctttagaag gtgtacctgc catggataaa aagaaaacag agatgttaca
     1081 acaaacctat tttgatcatg tttattttac ggaggaaaaa tcatgttaaa cgaatggaat
     1141 ggattcaacg atggtgtctg gacaaaagaa atcaatgtaa gaaattttat acagaaaaac
     1201 tacactctct atgaaggaga tgactctttc ttagctgatg tatctcagaa aacaaaaaaa
     1261 gtctggaata aatgtgaaga actgcttgct gaagaattga aaaaaggagg cgttttagat
     1321 gttgagacag atatcatttc tggtatcacg aacttcgcac ctggatatat tgataaagaa
     1381 aatgaggtga tcgttggact tcagacagat gcacccctaa aacgtattgt caatctatat
     1441 ggcggtaaac gtatggcgca ccagtcactg gaacaatatg gttataaact aaatgaagag
     1501 atcgaccgcc attttaatga atacagaaaa acacacaatg aaggggtttt tgatgcctat
     1561 ccaaaacgta ctcgtcttgc aagaactgct ggacttctaa caggacttcc tgatgcttat
     1621 ggacgtggac gtattattgg agactaccgt cgtgttgctc tttatggaat cgaccacttg
     1681 atcgaagaaa aacaaaaaga tctagacttt gaagatggac ctatgacaga agaccgcatc
     1741 cgtctgcgtg aagaaatttc tgagcagatc cgtgcccttt ctaaaatgaa agaaatggct
     1801 gcatcttatg gtgttgatat ttcaaaacct gcaaaaaatg cgaaagaagc tgttcaggcg
     1861 gtttatatgg gatatcttgc gggaacaaaa gaaaataatg gagctgcaac ttcccttgga
     1921 cgtatttcta cattccttga tatttatatt gaacgagatc ttaaaaatgg agtgatcaca
     1981 gaaacagaag ctcaggaatt gatcgatcaa tttatcatca aacttcgtct gacaagacat
     2041 ttaagaactc ctgaatatga cgaactattt ggtggggatc ctacgtggat cacggagtct
     2101 atcggtggag ttggtattaa tggaaaacct ctggttacta agaactcttt ccgttatctt
     2161 catactttat ataacattgg aactgctcca gagcctaact taactgtttt atggtctgaa
     2221 aaactgccag acaactttaa acatttttgc tctaaagttt ctattgatac ggattctatc
     2281 caatatgaaa atgatgatgt tatgcgccca gtctatgggg atgattatgc gatcgcttgt
     2341 tgtgtatctg caatgaaggt tggaaaacag acacagctgt tcggagcccg ctgcaatctg
     2401 gcaaaatcac tgctttatgc gatcaatgga ggaattgacg agaaaaaagg aatccaggtt
     2461 gttcctggaa tcgaaccgat cacagatgac gttcttgatt ttgacaaagt atgggaaaac
     2521 tataagaaag ttatgactta tgttgctgaa ttatatgttg atacagtaaa tatcattcat
     2581 tttatgcatg ataaatacgc atatgaagcc agccagtttg ctcttcatga tacaaactta
     2641 gagcgaatcg cagcttatgg aattgctggt ctgtctattg ccgcagactc tctatcagcg
     2701 atcaaatatg caactgtaaa accaatccgc aatgaaaatg gtgtagcaat tgactttgaa
     2761 accattgggg atttccctaa gtatggaaat gatgacgacc gcgcagatga tcttgcagtt
     2821 aacacagtaa cattcttctc tgacgaacta aagaaacatc cgatttatag aaatgcgatc
     2881 catactttat ctgctttaac gatcacatcc aatgttatgt atggtaagaa aaccggatcc
     2941 acaccagatg gaagaaaact cggagaacct ttagctccag gtgcaaaccc aatgcatgga
     3001 cgagacgaaa atggtgctct tgcatcctta aactctgtcg ctaaaattcc atatcgcgat
     3061 gtatgtcagg atggtgtatc caataccttt tccatcgtac cagatgctct tggaaaagat
     3121 caggaacaac gagtacaaaa ccttacaaca atcttagacg gatactttgt tcaaggtgca
     3181 catcatttga atgtaaatgt tatgcataga gaaactttga tcgatccgtg tttctggata
     3241 tgccgtgaac tttaatcgat tatcccgcga acagcaggaa gaagtgattc gtcgtacatt
     3301 ccatcagagt atgtagcttt ttatgaaaca gcaatggtgc ctgatttttt gatcgaatca
     3361 ggcaccatta ctattcttca aacatttctc cctctatatc acttatctgt tcaataggga
     3421 tgatcgtttg atctgtcata acaactgtgt gtttatattt atcaattttc tttacacaac
     3481 cagaacaggt aatatatgct cccccatctt tcttagcatc tggtacaaaa tacgtgatct
     3541 caaccatttg ctgtgtaccg atcgtttcag cgatcagata taacttttta tttaattctg
     3601 ctataacttc ttcactcaac ccctgttttg catctgtttg tcttacagtn nnnnnnnnnc
     3661 tttaatagct gcttcgtgtc cggtaagtgc cgcaaaaggc gaaaactgtg ctgccctggc
     3721 ataaagtgac atacgagggt gcttctttga agttggatgc ggcagattga taatatcatc
     3781 gtatttatga ttgtactgat ccttcataat cttcctccat cgacactgaa tacttacttt
     3841 ctatgcttta tgtccgccga tctgctcatt acggtctttt gctgttgctc cctcctgaag
     3901 gttcattcct ttcaagatag cgtttttccc aaacttcttt ttgattgtga gcattgtctg
     3961 ttgcatccgt ttctcacgct ctaaaacagc ctcttcttcg gcttgttcct gttcctttac
     4021 cgtataatct gtaaaaagat caagctgctc atacgtttct tcttcctttg aaaaactttc
     4081 atccacgagc ttgttcgctg tgatattgat tcttctgatc agcagatttt tatccacgat
     4141 ccgatcatat aattccatca ctgcatctgt gatcagcatg gtagatgatg tcttcctttt
     4201 caggtttgtt gttccatgag cgtgttttgg aactttcctt ccatatcggt cgatcgtaac
     4261 agatcccttg tacttcctat atctatctgg atccgttaga ttttctatat cataacctac
     4321 ggtcaatacg atctggtcag taacaagtct cttatctacc agatcaagtg tcataagatc
     4381 agtcatttct tttacaacta actttgcctt atcaaagtca tatgggcagt gaagtacttg
     4441 cccggagcat acactgttgg tttccggttt gtacgctttg acttgctcca tcgtacaagg
     4501 ctcatagccc caggcatgat cgatcagcag ttccgcattg atcccaaaca tcttataaag
     4561 cagttcttca ttatggaatt catttggctt tccgacggaa catcttgcaa catctcccat
     4621 tgagaaaaga ccgtgtt
//
LOCUS       DAOMUE010000159         4619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5217_length_4619_cov_3.074277, whole genome shotgun sequence.
ACCESSION   DAOMUE010000159 DAOMUE010000000
VERSION     DAOMUE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5217_length_4619_cov_3.074277"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..770)
                     /locus_tag="PLC71_07480"
     CDS             complement(237..770)
                     /locus_tag="PLC71_07480"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HRM02519.1"
                     /translation="MRNILIVVDMQNDFIDGSLGTKEAQEIVTPVAGKIRNFEGDIYG
                     TLDTHEEDYLSTQEGKNLPVKHCICGEDGWALNSEIMQAIAETKAEVHNFCRKGTFGS
                     MELAQILKETYEDQEVEIELIGLCTDICVISNAMLIKAALPEVKVSVDSSCCAGVTPE
                     SHRNALEAMKMCQIEIA"
     gene            complement(767..1213)
                     /locus_tag="PLC71_07485"
     CDS             complement(767..1213)
                     /locus_tag="PLC71_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRM02520.1"
                     /translation="MSMIDVIRKEMMTAMKNKDKERKESLSMLLSALKNAQIDKREDL
                     TEAEENTIIAKEIKQTKETMDTCPADRTDIIDECKARLAVLEEFAPEQMSEEQIEAEI
                     NAVLAELGIDAPTMKDKGKVMKNLMPRVKGKADGKLVNQLVGAKLS"
     gene            complement(1305..1913)
                     /locus_tag="PLC71_07490"
     CDS             complement(1305..1913)
                     /locus_tag="PLC71_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02521.1"
                     /translation="MHSIWQEQMEFPVYPILKRDKKTDILYVGATLKHAIEANFQKEM
                     GRSVMIIEEKSIGDMPELGGMGILKATNMNEFKELCILKNYIIRQHIPCDLEIISETS
                     IQVHPVKLFLFMTKDVEVYEQTKITARQGKRLDIESAYIDAGQVIEDDKPCEKRYIHV
                     FSEAGFPEITKPDILEIRKYKEDYLVLSYQRKLEGSRNSWEI"
     gene            complement(1966..3351)
                     /gene="asnS"
                     /locus_tag="PLC71_07495"
     CDS             complement(1966..3351)
                     /gene="asnS"
                     /locus_tag="PLC71_07495"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRM02522.1"
                     /translation="MEMMTGRTLFRNYKEYDQKEVEICGWVKSNRGSKKFGFLVINDG
                     TFFEPIQVVYDQEMGNFDTVSKLNVGTAVIVNGTVIVTPDAKQPFEIHAKSVEVEGEC
                     PSDYPLQKKRHTLEYLRSISHLRPRTNTFQAVFRVRSQIAYAIHKFFQERDFVYVHTP
                     LITGQDCEGAGEMFQVTTMDLNKIAEEGKVDYEQDFFKKPVSLTVSGQLAGETFAQAF
                     RNIYTFGPTFRAEDSNTTRHAAEFWMIEPEIAFADLEDNMILAEQMIKYIINYVMEHA
                     QEELQFFNKFVDKGLLERLNHVVSSDFGRVTYTEAVKLLEEHNDEFEYKVSWGIDLQT
                     EHERYLTEQIFKKPVFVTDYPKEIKAFYMKMNEDNKTVAAMDLLVPAIGEIIGGSQRE
                     DDLAKLEERITELGMKPEDYDFYLDLRKYGSTRHAGFGLGFERMVMYATGIANIRDVI
                     PYPRTVGKCQY"
     gene            complement(3377..3796)
                     /locus_tag="PLC71_07500"
     CDS             complement(3377..3796)
                     /locus_tag="PLC71_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HRM02523.1"
                     /translation="MEYKKFNNQYVIRIDKGEEICAKLKEVAQKENIKLAYLTGIGAA
                     GKVTAGVFDTKEKVFKGHTWEGDLEIVSIGGNINTMNGETYTHFHISVADEAGNVYGG
                     HLTEAVISGTGELVLTEIEGTVDRKFDEEIGLNLFEF"
     gene            complement(4026..>4619)
                     /locus_tag="PLC71_07505"
     CDS             complement(4026..>4619)
                     /locus_tag="PLC71_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HRM02524.1"
                     /translation="TFAWGLAVLVPAFIFGSSSGAHFNPALTIALAAIGNFSWSMVPG
                     YVIAQFAGAFVGAVLVYVLFAGQFEVTKDQGTKLGVFCTGPSIANTGLNLLSEIIGTF
                     VLVFAILGIGNVENAGSMGLNYFFVFAIIVSIGMSLGGLTGYAINPARDLGPRLAHAV
                     LPIPGKGDSNFGYAWIPVVGPIIGALLAGGLFVVIPW"
BASE COUNT         1237 a         1062 c          679 g         1641 t
ORIGIN      
        1 ttaattgaat attcaatcca ttactatccg ctgctttttt ctttagatat tcatctcgtg
       61 cataaagttt attgtttgtc ataggcatta ctcctttata tttttacatt tattgtaaat
      121 tttattatgc ccatgatttt gaaatacatc aagatattat tgtaaatatt tctattattt
      181 tacattttaa tttaaaataa aaaggcgact tcatatcgcc ctttatttac aactaattat
      241 gctatctcaa tctgacacat cttcatagct tccaacgcat tcctatgact ctcaggagtc
      301 actcctgcac agcatgaaga atcaacactt accttcactt ccggcagtgc tgctttgatc
      361 agcattgcat tggatatcac acagatatcc gtacataatc cgatcagttc gatttcaact
      421 tcctgatctt catatgtttc cttcaatatc tgtgccagtt ccatagaacc aaaagttcct
      481 tttctacaga aattatggac ttctgctttc gtctctgcga ttgcctgcat gatttcgctg
      541 tttaatgccc agccgtcttc tccacagata caatgtttga ccggaagatt ctttccttcc
      601 tgtgtggaaa gataatcttc ctcatgtgta tcgagcgttc catagatatc accctcaaaa
      661 ttacgaatct ttcccgcaac aggtgttacg atctcctgtg cttctttggt tccaaggctt
      721 ccgtcgatga agtcattctg catatcgacg acaattaata tatttctcat gaaagtttag
      781 ctcctaccag ctggtttaca agtttaccgt cagcttttcc tttgactctt ggcatcaggt
      841 tcttcataac ttttcctttg tctttcattg ttggcgcatc aattccaagt tctgctaata
      901 cagcattgat ctcagcttcg atctgttctt cgctcatctg ctctggtgca aactcttcta
      961 agactgctaa tcttgctttg cattcatcaa tgatatctgt acgatcagca gggcaagtat
     1021 ccattgtctc ttttgtctgt ttgatctctt ttgcgataat tgtgttttct tctgcctctg
     1081 tcagatcttc tctcttatcg atctgtgcgt tttttaatgc acttaataac atagaaagac
     1141 tttcttttct ctctttgtcc ttattcttca tcgctgtcat catttctttg cggattacat
     1201 caatcatact catagtttct tatcctcctg tataaatgtt ctcttgacat ctattgtagc
     1261 acaaaatact atcttcgttt cactaatttt acaacatgac tcaattatat ttcccaagaa
     1321 ttccggcttc cttccaattt tctctgatac gacaacacca gataatcttc tttatatttt
     1381 ctgatctcca gaatatctgg cttcgtgatt tctggaaaac ctgcttctga aaatacatga
     1441 atatatctct tctcacaagg tttgtcgtct tcgatcactt gtcctgcatc gatataggca
     1501 ctttcgatat ccagacgttt cccctgtctt gcagtgatct ttgtctgttc atatacttca
     1561 acatccttcg tcataaacaa aaacaacttc actggatgga cctgaatgct tgtctctgaa
     1621 ataatttcaa gatcacatgg aatatgttgt cttataatat aatttttcaa aatacagagc
     1681 tctttaaatt cattcatgtt cgttgctttc aaaatcccca tgccaccaag ttctggcata
     1741 tctccaatac ttttttcttc aatgatcata actgatcttc ccatttcttt ttgaaaattt
     1801 gcctctattg catgtttcag ggttgcacct acatataaaa tatctgtttt tttatctctt
     1861 tttaaaatcg gataaactgg aaattccatc tgttcctgcc aaatagaatg catatactac
     1921 tacctcctaa aaattgctct cgtaatagta tatgcataat tcatcttaat attgacattt
     1981 tcctactgtt cttgggtatg gaatgacatc tctgatatta gcaattcctg ttgcgtacat
     2041 taccatacgt tcaaatccaa gaccaaatcc tgcatgtctt gtggaaccat atttgcgaag
     2101 atctaaataa aaatcataat cttctggttt catacctaat tctgtgattc tctcttccag
     2161 tttcgcaaga tcatcttctc tctggcttcc cccgatgatc tctccaatag ctggcactaa
     2221 aagatccatg gctgcaaccg tcttgttgtc ttcattcatc ttcatataaa atgctttgat
     2281 ctcttttgga tagtctgtga caaacactgg tttcttaaag atctgctctg tcagataacg
     2341 ttcatgctct gtctgaagat caattcccca agatacttta tattcgaatt catcattgtg
     2401 ttcttctaat aatttcactg cttctgtata agtaactctt ccaaaatcag aagatactac
     2461 atggttcaaa cgttctaata atcctttatc tacgaattta ttaaagaact gtaattcttc
     2521 ctgtgcatgc tccatcacat aattgatgat atatttgatc atctgctctg caaggatcat
     2581 attatcctca agatctgcaa atgcgatctc tggctcgatc atccagaatt ctgctgcatg
     2641 tcttgttgta ttggaatctt cagctctgaa agttggtcca aatgtataaa tattgcggaa
     2701 tgcctgtgca aaagtctctc ctgcaagctg tccgctgacg gttaagctga ctggcttctt
     2761 aaagaaatcc tgttcataat caacttttcc ttcttcagcg atcttgttaa gatccatcgt
     2821 tgttacctgg aacatctctc cagcaccttc acagtcctgt cctgtgatca atggtgtatg
     2881 cacgtataca aaatcacgtt cctgaaagaa tttatggatc gcatatgcaa tctgagaacg
     2941 aactctgaat actgcctgaa acgtattcgt tcttggtctt aaatgagaga tagatcttaa
     3001 atattctaat gtatgacgtt ttttctgtaa tggataatcg gatgggcatt ctccctcgac
     3061 ttctacactc tttgcatgaa tctcaaatgg ctgtttcgca tctggtgtca cgatcacagt
     3121 tccattaacg atcacagctg ttccaacatt taatttgctg accgtatcaa agttgcccat
     3181 ctcctgatca tatacaacct gaatcggttc aaaaaatgta ccatcattaa taaccagaaa
     3241 tccgaatttc tttgatccac gattgctttt tacccagccg cagatctcta cttctttctg
     3301 atcatattct ttgtaatttc gaaataatgt tcttcctgtc atcatttcca tgttatcttt
     3361 gtcctccgtt tttcacttag aattcaaata aattcagccc gatttcttca tcaaacttgc
     3421 ggtcaactgt gccttcgatc tcggttaata caagttctcc ggttccgctg atcactgctt
     3481 ctgtcagatg acctccatag acattccctg cttcatccgc aacactgata tggaagtgtg
     3541 tataagtttc cccattcata gtattgatat tcccaccaat ggaaacgatc tcaagatcac
     3601 cttcccatgt atggcctttg aatacttttt cctttgtatc aaaaacacca gctgttactt
     3661 tgcctgctgc cccgattcct gttaagtatg caagtttgat attttctttt tgggcgacct
     3721 cttttaattt tgcacagatt tcttcgcctt tgtcaattct tattacgtat tgattgttga
     3781 attttttgta ttccatgttc attacctcct tttggctctt ataatcctgc atatatttta
     3841 ccgcacgtgc tatttctttt ctaggtgttt tcatagattt cttagtgaca tcctctccca
     3901 cctataggac gccactgaaa aaagataaaa ccatttaata atatatatct tgtaaaaaaa
     3961 tggatgcctc cttctaagaa aacatccatc ttttcaatct ctattttatt ttcgtttatc
     4021 taatactacc atggaatgac tacaaataat cctcctgcga gcagtgctcc aatgatcggt
     4081 cctacaacag gaatccatgc atatccaaag ttagaatccc cttttcctgg gattggaaga
     4141 actgcatgtg caagtcttgg gcctaaatct ctggctggat taatcgcata tccagttaat
     4201 ccaccaagtg acataccaat ggaaacgatg attgcaaata cgaagaaata gttaagtccc
     4261 atagaacctg cattttccac atttccgatt ccaagaatcg caaaaactaa aacaaaggtt
     4321 ccaatgatct cacttaacag gtttaatcct gtgtttgcaa tagaaggtcc tgtacaaaat
     4381 actcctaatt ttgttccctg atctttggtt acctcaaact gccctgcaaa taagacatac
     4441 acaaggactg ctcctacaaa tgctcctgca aactgagcga tcacatatcc tggcaccata
     4501 gaccaagaga aattgccaat agccgcaagt gcgatcgtaa gtgccgggtt aaaatgagct
     4561 ccagatgaag atccaaaaat aaatgctggt acaagcaccg caagtcccca tgcaaatgt
//
LOCUS       DAOMUE010000160         4612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5233_length_4612_cov_1.983761, whole genome shotgun sequence.
ACCESSION   DAOMUE010000160 DAOMUE010000000
VERSION     DAOMUE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5233_length_4612_cov_1.983761"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLC71_07510"
     CDS             complement(<1..241)
                     /locus_tag="PLC71_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HRM02525.1"
                     /translation="MLTSIIGFPRVGNLRELKFATEKYFKHQISQERLQTVAKEIREK
                     QWRLLKENGIDLIPSNDFSFYDTLLDSAALFNIIPS"
     gene            537..1100
                     /locus_tag="PLC71_07515"
     CDS             537..1100
                     /locus_tag="PLC71_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HRM02526.1"
                     /translation="MDYQSYLSHNVAVNLKRIRTSKGMSLDVLSEQTGVSKSMLAQIE
                     KGTANPSLGVLGKITSGLRIEFQELIDAPPMESCLVTPDQMTPTKEMIGEYKVWTCFP
                     YEDNHQLEIYRIDIEPGGKYISGSHGEKTREYLSVTDGELTIECGEDVQKITKGEIYR
                     FETDKKHNYKNETQQKTSFICVFVDYR"
     gene            1478..2692
                     /locus_tag="PLC71_07520"
     CDS             1478..2692
                     /locus_tag="PLC71_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HRM02527.1"
                     /translation="MRLKDTGLTSQDIKDKVNKYMIETYERFDFLAEKAKDMYMYDEN
                     GEEYLDFYAGIAVNNAGSCNEKVVAAVKDQVGDIMHTFNYPYTIPQALLAEKVCETIG
                     MDKIFYQNSGTEANEAMIKMARKYGIEKYGPNHYHIVTAKMGFHGRTFGAMSATGQPG
                     NGCQVGFGPMTYGFSYAPYNNLQAFKDACTENTIAIMIEPVQGEGGVHPATQEFMKGL
                     REFCDENDMLLLIDEVQTGWCRTGAVMSYMNYGIKPDIVSMAKGLGGGMPIGAICATA
                     EVAKAFTPGSHGTTFGGHPVSCAAALAEVNELIDRDLAGNAKKVGDYFAEKLKGLPHV
                     KEVRHQGLLTGIEFDDTLNAVDIKHACFDRKLLVTAIGSSVIRTIPPLIVTEEECDKA
                     FAIMKEAVESLA"
     gene            2738..3334
                     /locus_tag="PLC71_07525"
     CDS             2738..3334
                     /locus_tag="PLC71_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRM02528.1"
                     /translation="MKHFIINIGCEYGSGGPDIGKMVAESLGIPFYDRDLVDQVVEKL
                     GVDRDLVEKADSGEENVKYEFDTTFGPRYANLTNRVIYTQFEVIHKLAAKSSCVIIGR
                     CSNYILKDRDDCLNIFVYAPEEYKVKHIMEQKGVSQKEAQKLVKYNDGMLKSRYEYMT
                     NSDMRDCRTRHMMIDSSILGLEKTAKYIMQLIDLRFEN"
     gene            3446..>4612
                     /locus_tag="PLC71_07530"
     CDS             3446..>4612
                     /locus_tag="PLC71_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRM02529.1"
                     /translation="MEKKKSEFDKVFSAWDILVIAFGAMIGWGWVVSTGDWIGRGGVL
                     GAVIGFAIGGIMVFFVGLTYAELTAAMPQCGGEHVFSYKAMGPVGSFICTWAIILGYV
                     SVVCFEACALPTIITYIYPGFLKGYLYTVAGFDIYASWLAVAMIVAFFITFINIKGAK
                     TAATLQTVLTVIIGGVGILMIVASVVSGDASNLTPQLFAGDSASTTMKAIMSVAVMTP
                     FFFIGFDVIPQAAEEINVPLKKIGMIMILSIVLAVAFYALIILGVGYVMSPSDISSSQ
                     AGSGLVTADAMAKAFHSSIMSKVLIVGGMCGIVTSWNSFLIGGSRAMYSMAESYMIPR
                     TFRKLHKTHKTPVNALYLIGGLSILAPLLGRKMLVWIVDAGNFGCCLAYCMVSLS"
BASE COUNT         1624 a          696 c          977 g         1315 t
ORIGIN      
        1 gagatggaat gatattaaac aaagctgcac tgtcaagcaa tgtatcgtaa aatgagaaat
       61 cattggatgg gatcaggtca attccatttt cttttaataa tctccattgt ttttcacgga
      121 tttctttcgc aactgtttgc aaacgttcct gtgagatctg atgtttaaaa tatttttctg
      181 tggcaaattt tagttctctt aagtttccaa ctcttgggaa accaatgatt gatgttaaca
      241 tagttgtctt ctccttttgg tgtgattgtc gatttgtttt atgggcttta ttatagttga
      301 ggatttagaa agtgtgaaat acataaaaat gtgagtttga tataatctga agttataatg
      361 atgtagaaga agacgtctta tcagatgagt tagaaacatt aaaaaaagga atggttatgt
      421 aaaaaattgc acttgcatta aagcagtatt gcagataaca taaggatagt aataatactt
      481 gcattagaac acgcagaaat gaagaaactg ataataaaag caaaaaggag tctcctatgg
      541 attatcaatc atacctatct cacaatgtag ctgtcaacct aaaaagaatt cgaacttcca
      601 agggcatgag tcttgatgta ctatcagaac aaacaggtgt tagtaaaagt atgttagctc
      661 agattgagaa aggaacagca aatccatcgt taggtgtttt aggaaagatc acaagcggat
      721 taagaatcga attccaagaa ttaattgatg ctccgccaat ggaatcctgc cttgtaacac
      781 cagatcagat gacaccaaca aaagaaatga tcggtgaata caaagtatgg acatgttttc
      841 catatgaaga taatcaccaa ttagagattt accgcattga tatcgaacca gggggcaaat
      901 atatcagtgg cagccatgga gagaagacaa gagaatatct ttcagttaca gatggagaac
      961 ttacgattga gtgtggtgaa gatgttcaga agatcacaaa gggggaaatc tatcgatttg
     1021 aaacagataa aaaacataat tataaaaatg aaacacaaca aaagacaagt tttatctgtg
     1081 tgtttgtaga ttatcggtaa gtatcatacg aaagtagtaa gatgataaaa tgatatgttc
     1141 aatataatgg acagatgtaa tatataatat tttacaaaaa aattaaaaaa ataataaaac
     1201 actaagaaaa aatatacaat ataataataa aatatattat aaaagataaa aataaagaaa
     1261 tttcaaaaaa taacaaagat atattgacaa ttcaatcctc tagtgttatt gtatagttgt
     1321 acaaaataaa ggatagaaat taagcaaggg cgcttaccaa caggataagt ttcccttgct
     1381 tttcttttta ttatttaaag tttatcaata aaaaagataa acaaaatctg gcccagagaa
     1441 gttaaataac taataacatc atgaggagga agcagatatg agattaaaag atacaggatt
     1501 aacaagtcag gatattaaag ataaagtaaa caaatatatg atcgagacat acgaacgttt
     1561 tgatttcctt gcagagaaag caaaagatat gtatatgtac gatgaaaatg gtgaggaata
     1621 tctcgacttc tatgcaggaa tcgcagtaaa taacgcagga agctgtaatg aaaaggttgt
     1681 agcagcagtt aaagatcagg ttggcgacat catgcataca ttcaactatc catacacaat
     1741 cccgcaggca ctgcttgctg aaaaagtatg tgaaacgatt ggaatggata agatcttcta
     1801 tcagaactct ggtacagagg caaatgaagc aatgatcaag atggctcgta aatacggaat
     1861 cgaaaaatac ggaccaaacc attatcatat tgtaacagcg aagatgggat tccatggaag
     1921 aacattcgga gcaatgtcag caacaggaca gccgggaaat ggatgtcagg tcggatttgg
     1981 accaatgact tatggattct catacgcacc atacaataac cttcaggcat ttaaagatgc
     2041 atgtacagaa aatacgatcg cgatcatgat cgagccagta cagggtgaag gtggtgttca
     2101 tccagcaaca caggaattca tgaaaggatt aagagaattt tgtgatgaaa atgatatgtt
     2161 actcttgatc gatgaagttc agacaggatg gtgtcgaaca ggtgcagtga tgtcttatat
     2221 gaactatgga atcaaaccag acatcgtatc catggcaaaa ggcttaggtg gaggaatgcc
     2281 gatcggagca atctgtgcaa cagcagaagt tgctaaagca tttacacctg gatctcatgg
     2341 aacaacattt ggtggacatc cagtcagctg tgcagcagca ttagcagaag ttaatgaact
     2401 gatcgatcgc gacttggctg gaaatgcaaa gaaagttgga gattactttg ctgagaaatt
     2461 aaaaggactt cctcatgtaa aagaggtcag acaccaggga ttattaacag gtatcgaatt
     2521 tgatgacaca ttaaacgcag ttgatattaa acatgcatgt tttgatcgaa aactgttagt
     2581 aacagccatc ggaagcagtg tgatccgtac aatcccacca ttgatcgtaa cagaagaaga
     2641 atgtgataaa gcatttgcga tcatgaagga ggctgtagaa tccttagcat aaaataatat
     2701 ctttgaaaaa agatcaaaat atcagggagg aatgtaaatg aaacatttta tcataaatat
     2761 cggatgtgaa tacggaagcg gtggtccgga cattggaaag atggttgccg aatcacttgg
     2821 aattccattt tacgacagag atttagtaga tcaggtcgta gagaaacttg gagttgacag
     2881 ggatctggta gaaaaagccg attccggaga agaaaatgtc aaatatgaat ttgacacaac
     2941 ctttggacca agatatgcga atcttacaaa ccgtgttatt tacacacagt ttgaggttat
     3001 tcataaactg gcggcaaaat catcttgtgt cattatcgga agatgtagta actatatatt
     3061 aaaagacagg gatgactgct taaatatctt tgtatatgca ccagaagaat ataaagtgaa
     3121 acatatcatg gaacaaaaag gtgtatcaca aaaagaggca caaaaacttg taaaatataa
     3181 tgatgggatg ctgaaatcaa gatatgaata tatgacaaac tctgatatga gagattgccg
     3241 taccagacat atgatgatcg attctagtat tcttggactt gaaaaaacag caaaatatat
     3301 catgcagtta attgacctgc gatttgaaaa ctaaaataaa atacatttac attctaacaa
     3361 aggcgttaag agataaggat cttttagcgc cttttttctt tcgaaacttt aaacagaatg
     3421 tgaagcaaga aaggaatgat atataatgga aaagaaaaaa tcagaatttg acaaagtatt
     3481 tagtgcatgg gacattcttg tcattgcatt tggcgctatg atcggttggg gatgggttgt
     3541 ttcaaccgga gactggatcg gtcgaggcgg agttttagga gctgtcatcg gttttgcgat
     3601 cggtggtatc atggtattct ttgtaggact tacttatgca gaacttactg cagcaatgcc
     3661 acagtgtgga ggagaacatg tatttagtta caaggcaatg ggaccggttg gttcatttat
     3721 ctgtacgtgg gcgatcattt taggatatgt cagtgttgta tgttttgaag catgtgcact
     3781 tcctacaatt atcacgtata tttatcctgg attcttaaaa ggatatctgt atacagttgc
     3841 aggatttgat atttatgcat catggcttgc agttgcgatg atcgtagcat tctttatcac
     3901 atttatcaat atcaaaggtg cgaagacagc tgcaacttta cagactgttt taacagtgat
     3961 cattggtgga gttggaatcc ttatgatcgt agcatctgta gtcagtggag atgccagcaa
     4021 tttaacacca caattatttg caggagatag tgcgtcaact actatgaaag cgatcatgag
     4081 tgttgcggtt atgacaccat tcttctttat tggatttgat gttatcccac aggcagcaga
     4141 agagatcaat gtgccgctta aaaaaatcgg tatgatcatg atcttatcta tcgtactagc
     4201 agttgcattt tatgcgctga tcatcttagg tgttggttat gttatgagtc catctgatat
     4261 ttcatcttca caggcaggat caggacttgt tacagctgat gcaatggcaa aagcattcca
     4321 cagcagtatc atgtcaaaag tattgatcgt cggtggtatg tgtggaatcg ttacaagttg
     4381 gaactccttt ttaattggtg gtagccgtgc aatgtattcc atggctgaat cttatatgat
     4441 tccaagaact ttccgtaaat tacataaaac acacaaaaca ccagtgaatg cattatactt
     4501 gatcggtgga ttatccatat tagctccatt acttggacgt aaaatgttag tatggatcgt
     4561 cgatgcaggt aactttggat gctgtcttgc atactgtatg gtttccttat ct
//
LOCUS       DAOMUE010000161         4611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5236_length_4611_cov_2.135206, whole genome shotgun sequence.
ACCESSION   DAOMUE010000161 DAOMUE010000000
VERSION     DAOMUE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5236_length_4611_cov_2.135206"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..267
                     /gene="xseB"
                     /locus_tag="PLC71_07535"
     CDS             58..267
                     /gene="xseB"
                     /locus_tag="PLC71_07535"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRM02530.1"
                     /translation="MAKKKFDIDQGFHQLDEILTKLSSEEIKLPEAVKLYTEGVNLVK
                     QCKDSLDKVEKELIVLEENGEEHEL"
     gene            257..1150
                     /locus_tag="PLC71_07540"
     CDS             257..1150
                     /locus_tag="PLC71_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRM02531.1"
                     /translation="MNFKDEMKQKVAQIEIILDEYLPAQEGLQKTVLTAMNTTVKAGG
                     KRLRPMLINETYQMFGGKGDIVKPFMAAIEMIHTYSLIHDDLPALDNDDYRRGQKTCH
                     IVYGEDMAILAGDALLNYAYEVATKAFDLAEKDQIMNVVEAIKILASKPGIYGMIGGQ
                     VADVELEGTPLSMEQILFIHKNKTSALIEACMMIGAVLAGASKEDVLKMEECGEYIGL
                     AFQIQDDILDLTGDEEEIGKPVGSDEKNHKTTYVTLKGLERSQRDVEDISKKAIDILE
                     KYDKGDCYLTNLTRFLIHRTH"
     gene            1174..3045
                     /gene="dxs"
                     /locus_tag="PLC71_07545"
     CDS             1174..3045
                     /gene="dxs"
                     /locus_tag="PLC71_07545"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRM02532.1"
                     /translation="MLEYINDPNDIKQIQPEDYKLLAKDIRRFLLRNVSRTGGHLASN
                     LGIVELTMALHLVLDFPKDQLIFDVGHQSYVHKILTGRKNGFENLRQFHGMSGFPKRK
                     ESDCDAFHSGHSSMSLSAALGMVTARDINHTDETIVAVIGDGALSGGMAYEALNNMAR
                     LRKEKKNLIIILNDNKMSIAENVGGMSAYLNKVRTRKEYVEFKGNVEQSLLRIPGIGK
                     ELTTILKKSKDSIKQLFVPGMYFEDMGITYVGPIDGHNVPLMVDTLNRAKQLDEPIII
                     HVVTKKGKGYRPAEQNPAKFHGISPFSLKTGEVLKKSDNPSNTAVFSDTLIKEAKKDK
                     KIVAVTAAMPDGTGLGKFKNHFPDRFFDVGIAEQHAVTFCAGMASRGLKPVFAVYSTF
                     LQRGFDQILHDVAIGNYSVIFGLDRSGLVGADGETHQGIFDIPYLSIIPNMTVMAPIN
                     GRELSEMLKHTLHHAKGPTAIKYSRGEASSLYEDQFEELHYGKGQILKEGKEAVIIAV
                     GNIFEEADKAEKILKEEGYEIGLVNPRYIKPFDQELIMKLSQTYDKIMIAEEGVLNGG
                     FGMMVNEFLSEHDYKGEVRCLGIDDQFVEHGSVSELRRMLKIDGESIADSIRQWMKE"
     gene            3050..3871
                     /locus_tag="PLC71_07550"
     CDS             3050..3871
                     /locus_tag="PLC71_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRM02533.1"
                     /translation="MKKERLDVLLVKKGLASSREKAKAIIMSGIVFVDGQREDKAGST
                     FDEKQEIIVKGKTLKYVSRGGLKLEKAMKNFDIVLKDKVCMDVGASTGGFTDCMLQNG
                     AVKVYSVDVGHGQLDWKLRNDERVVCMERTNMRYMTEDDIDEKASFVSIDVSFISLTK
                     ILPAVYRILNTGGEVVALIKPQFEAGREKVGKKGVVRDPKVHEEVIEKICDFASTNGF
                     ELLHLDYSPIKGPEGNIEYLLHMRKTETMENGHTMTDQIKEVVDASHADLAKNKE"
     gene            3873..>4611
                     /locus_tag="PLC71_07555"
     CDS             3873..>4611
                     /locus_tag="PLC71_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRM02534.1"
                     /translation="MKNFLILTNEKKDPGLRISKKIQDYIEKQGGISQRMCDFTRHVQ
                     KDMNCITKDTECVIVLGGDGTMLHAARLIVDHDIPMVGVNLGTLGFLTEIELSKLYDG
                     LDGLLNDTFQIEERMMLDGRVIHADHETDHLPALNDVVIARSGFSRIISFRIMVNGKL
                     LDVYEADGIIVSTPTGSTGYNLSAGGPVVNPKANVILITPICPHSLQSNSLVLSPEDE
                     IDIYIENVRESQLEEAYVTFDGQVARKL"
BASE COUNT         1580 a          666 c         1140 g         1225 t
ORIGIN      
        1 ggcaaaacag atcagaacaa tggatgaaaa agggaagtaa gaaagaggaa gtttattatg
       61 gcaaagaaga aatttgatat tgatcagggt tttcatcagt tagatgaaat tcttacaaag
      121 ctttccagtg aagaaatcaa acttccagag gctgtcaaac tatatactga aggagtaaat
      181 cttgtgaaac agtgtaagga ttctctggat aaagtagaaa aagaattgat cgtattagaa
      241 gagaatgggg aagaacatga actttaagga tgagatgaaa caaaaggttg cacagattga
      301 aattatttta gatgaatatc tgccagcaca ggaagggtta caaaaaacag ttttaactgc
      361 aatgaacaca accgttaagg cgggaggaaa gcgtcttcgt ccaatgctga tcaacgagac
      421 atatcagatg tttggcggaa agggagatat cgtaaagcca tttatggcag cgattgaaat
      481 gatccatacg tattctctga ttcatgatga tcttccagca cttgacaatg atgattacag
      541 aagaggtcag aaaacatgtc atatcgttta tggagaagat atggcgatcc ttgcaggaga
      601 tgcacttctt aattatgcgt atgaagttgc aacgaaagca tttgatcttg cggagaaaga
      661 ccagatcatg aatgtagttg aagcgatcaa gattctcgcc agcaaacctg gaatctatgg
      721 aatgatcggc gggcaggttg cagatgttga actggaaggt acaccgctta gtatggagca
      781 gattttattt attcataaga ataagacgtc tgcattgatc gaagcatgta tgatgatcgg
      841 agcagttctt gcaggagcat ccaaagaaga tgttctgaag atggaagaat gtggagaata
      901 tatcggactt gcgtttcaga ttcaagacga tattctggat cttacaggag atgaagaaga
      961 gatcgggaaa ccggttggaa gtgatgaaaa gaatcacaaa acaacttatg tgacattaaa
     1021 aggattagaa cgttcacaaa gagatgtaga agatatttca aagaaagcaa ttgatatttt
     1081 agaaaaatat gataagggag attgttatct tacaaatctt acaagatttt tgatccatcg
     1141 aacacactaa ggcagagagg aagagggagc attatgctag agtatattaa tgatccgaat
     1201 gatattaaac agattcagcc agaggactat aaacttctgg cgaaagatat acgtcgtttt
     1261 ctattaagaa atgtcagtcg cacagggggg catctggcat cgaatcttgg aattgtagaa
     1321 ctaacgatgg cgctgcatct tgttttggat tttccaaaag accagctgat ctttgatgta
     1381 ggacatcagt cttatgtgca caagatctta acaggaagaa agaatggatt tgagaatctg
     1441 aggcaatttc atgggatgag tggattcccg aagagaaaag aaagcgattg tgatgcgttt
     1501 cacagtggac acagctccat gtcgttatca gcagcgcttg ggatggttac agcaagagat
     1561 atcaatcata cggatgagac gattgttgcg gtgatcggag atggtgcgtt gtctggcgga
     1621 atggcatatg aagcattaaa taatatggca agactacgca aggagaagaa gaatctgatc
     1681 attatcctga acgataacaa gatgtctatt gcggagaatg ttggtggaat gtctgcgtat
     1741 ttgaataaag tacgaacaag aaaagaatat gtggaattta aaggaaatgt ggaacaatcc
     1801 ttattaagaa ttccgggaat cgggaaggaa ttaacaacaa ttcttaagaa atcaaaagat
     1861 tcgatcaaac aattatttgt tccgggaatg tattttgaag atatggggat cacgtatgtt
     1921 ggacctattg acgggcataa tgttcccttg atggtagaca cgttaaatag agcgaaacaa
     1981 ttagatgagc cgatcattat tcacgtggta acgaagaaag gaaaaggata tcgtcctgcg
     2041 gagcagaatc cagccaaatt tcacggaatc agtccatttt ccttaaagac aggggaagtt
     2101 ttaaagaaat cggacaatcc atcgaacaca gcggtatttt cagatacact gatcaaagag
     2161 gcgaagaaag ataagaagat tgttgcggta actgccgcaa tgccggatgg aacaggtctt
     2221 ggaaagttta aaaatcattt tccagatcgt ttctttgatg tagggattgc cgaacaacat
     2281 gcagttacat tttgtgccgg aatggcatca agaggtctaa agcctgtatt tgcagtgtat
     2341 tcaacatttc tacaaagagg ttttgatcag atcttacatg atgtggcaat tgggaattat
     2401 tcagttatat ttggattgga ccgctcagga cttgttggag cggatggaga aactcatcaa
     2461 ggtatttttg atattccgta tttatctatc atcccaaata tgacggtgat ggctccgatc
     2521 aacggaaggg aattatctga gatgctaaaa catacgctcc atcatgcaaa aggaccaacc
     2581 gcgatcaaat attccagagg agaagcaagc tctttgtatg aagatcaatt tgaagagtta
     2641 cactatggaa aaggccagat acttaaagaa ggaaaggaag ctgtgatcat tgcagttgga
     2701 aatatctttg aagaggcaga taaggcggaa aagatcctta aagaagaagg atatgagatt
     2761 ggtttagtta atccaagata tatcaaacca tttgatcagg agttgatcat gaaactgtca
     2821 cagacctatg ataagatcat gatcgcagaa gaaggcgtat taaacggcgg atttggaatg
     2881 atggtgaatg aatttctgag tgagcatgat tacaaaggtg aagtcagatg ccttggaata
     2941 gatgaccagt ttgtggaaca tggttctgta agtgagttaa gaagaatgtt gaagattgat
     3001 ggagaaagta ttgcagactc catcagacaa tggatgaagg aatagaaaaa tgaagaaaga
     3061 acgtttagat gtactgcttg tcaagaaagg gcttgcgtct tccagagaaa aagcaaaagc
     3121 gatcattatg tcaggaatcg tttttgtaga tggacagaga gaggataaag caggatcaac
     3181 ttttgatgaa aagcaggaga tcattgtaaa gggaaaaact ttaaaatatg tcagtcgagg
     3241 tggattaaag cttgagaagg ctatgaagaa ctttgatatt gtattaaaag acaaagtctg
     3301 catggatgtt ggagcatcta caggtggatt tacagactgt atgttgcaaa atggtgcagt
     3361 gaaagtatat tcagttgatg tcgggcatgg acagttggac tggaaactaa gaaacgatga
     3421 gagagttgtt tgtatggaac gtacgaatat gcgttatatg acggaagacg atatcgatga
     3481 aaaggcatcg tttgtatcta ttgatgtttc ttttatttca ctgaccaaga ttcttccagc
     3541 ggtatatcga attttgaata cgggaggaga agttgttgca ctgatcaaac cgcagtttga
     3601 agcaggaaga gaaaaagttg gaaagaaagg tgttgtacga gatccaaaag ttcatgaaga
     3661 agtcattgag aagatctgtg atttcgcatc tacgaacggg tttgaattat tacaccttga
     3721 ttattctccg atcaaaggtc cagagggaaa tatcgagtat ttattacata tgagaaaaac
     3781 agagacaatg gagaatggac atacaatgac agatcagatc aaggaagtgg tggatgcatc
     3841 acatgcggat ctggcaaaga ataaggaata acatgaagaa ttttttgatt ttaacgaacg
     3901 agaaaaaaga tccaggattg cgtatttcca aaaagattca ggattatatt gaaaaacaag
     3961 gtggaatcag ccagagaatg tgtgatttta caagacatgt tcaaaaagat atgaactgca
     4021 tcacaaaaga cacagagtgt gtgatcgttt taggtggaga tgggacgatg cttcatgcgg
     4081 caagattgat cgtagatcat gatattccga tggttggagt aaatctcggt accttaggat
     4141 tcttaacaga gattgagtta tctaaacttt atgacggatt ggacggattg ttaaatgata
     4201 ctttccagat cgaggagaga atgatgttag atgggcgcgt gatccatgcg gatcatgaga
     4261 cagatcatct tccagcactc aatgacgtcg tgatcgcaag aagtggtttt tcaagaatca
     4321 tctcgtttcg tattatggtc aatggcaaat tgttagatgt atatgaagca gatgggatta
     4381 ttgtttcgac gccgacagga tctacaggat ataatttatc agcaggtggg ccggtagtga
     4441 atccaaaagc aaatgttatt ttgatcactc cgatctgtcc acattcgctg cagtcaaaca
     4501 gtcttgttct ttctccagaa gatgagattg atatttatat tgagaacgtg agagaatctc
     4561 agttagagga agcctacgtt acctttgacg gtcaggttgc gagaaaactt c
//
LOCUS       DAOMUE010000162         4590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5261_length_4590_cov_2.532745, whole genome shotgun sequence.
ACCESSION   DAOMUE010000162 DAOMUE010000000
VERSION     DAOMUE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5261_length_4590_cov_2.532745"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="PLC71_07560"
     CDS             complement(<1..703)
                     /locus_tag="PLC71_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRM02535.1"
                     /translation="MEETISKNFIEQIIEKDIEEGHCKKVVTRFPPEPNGYLHIGHAK
                     SILLNYGLAQEYGGDFHFRFDDTNPTKEKEEYVNSIKEDVEWLGADYHEHIYYASNYF
                     DKMYECAEFLIKKGKAYVCDLNAEQIREYRGTLTEPGKNSPYRDRTPEENLQLFREMK
                     EGKYPDGSKVLRAKIDMTSGNINMRDPILYRIARMEHHNTGNKWCIYPMYDFAHPLED
                     AFEGVTHSICTLEFED"
     gene            complement(804..2159)
                     /gene="radA"
                     /locus_tag="PLC71_07565"
     CDS             complement(804..2159)
                     /gene="radA"
                     /locus_tag="PLC71_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRM02536.1"
                     /translation="MAKQKTVYFCSQCGHESSKWMGQCPACKQWNTFTEEKVTETKKG
                     GAKSLKTSASPMNISEVTVENEERIPTGIHELDRVLGGGIVKGSLSLVGGDPGIGKST
                     LLLQVCRNLANSKRKVLYISGEESMHQIKMRAERIGTFEEEMLLYCETDLDAITNAIL
                     KTKPEFAVIDSIQTMYSEDLSSAAGSVSQVREVTAAMMRVAKENNIAVFIVGHVTKEG
                     VVAGPRTLEHMVDTVLYFEGEREAAYRILRGVKNRFGSTNEIGVFEMCNNGLVEVENP
                     SKTMLNGRPLDASGSVVVWSMEGTRPILIEIQALVSPTSFQMPRRTAVGIDYNRVNLL
                     MAVLEKRVGLQLGGCDAYVNLAGGMKLGEPAIDLGIIMAIASSYKNRPILEDTIIFGE
                     VGLVGEVRAVSGGEARIKEAQKLGFKRCILPQANVDQIKVQTDMRLVGVSNVMEALDL
                     I"
     gene            complement(2178..2591)
                     /locus_tag="PLC71_07570"
     CDS             complement(2178..2591)
                     /locus_tag="PLC71_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endosialidase"
                     /protein_id="HRM02537.1"
                     /translation="MAVVTELFRSEDNGTLSFGDYTLAQKAKKGDIQFEGDIYKIKTF
                     KTMTKLDKNGQLIFESVPGSAVHNFVATTDGVAFEIESSSDVQVTLEVEAGQEYKVFI
                     DNTNVGMMKANLGGKLTLSVELSEGTAAAVRVKKQ"
     gene            complement(2659..>4590)
                     /locus_tag="PLC71_07575"
     CDS             complement(2659..>4590)
                     /locus_tag="PLC71_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRM02538.1"
                     /translation="TDLTEKAMLGKLDPMIGRESEMERLMQILCRRTKNNPCLIGDPG
                     VGKTAIVEGLAQRIAEGNMPRILKNKRILSLDLTKVIAGTKFRGEFEERMKRIVTEAT
                     QDDSIILFIDEIHTIIGAGGSEGAMDASNILKPALARGDFQLIGATTSEEYTKYFEKD
                     AALVRRFQPVRVKEPTVEETITILKGIKHRFEDYHRILVSEDVAEAIASLSDRYISDR
                     FLPDKAIDLLDEACARKRMEQLGSHAGFKKERKEIREIIEKIEAALSDGDITEARELK
                     KEKNKLEKSLERKMKRNQKKQNEIPELTTEDAAEVVSLWTQIPVTQLTKGDMERLRHL
                     EKELHKHVIGQDEAVNAVAKAVKRSRVGLKSPNRPIGSFLFLGPTGVGKTELSKTLAK
                     ALFGREEDLIRVDMSEYMEKHSVSKIIGSPPGYVGFGEGGQLSEQIRRHPYSVLLFDE
                     IEKAHPDVFNILLQVLDDGHITDSNGRKVDFKNTVIIMTSNAGANRIIAPKHLGFSMD
                     DTDKAKTHERMKKGVMEEVKQIFRPEFLNRIDETIVFAVLTKEEVKKIAKLFMDELRA
                     RLLSEHQITLKITSGAMDLLADKGYDENYGARPLRRIIQNEIEDVLADKILEGTLTNG
                     QTMTIGKKDKKLTFSVKEN"
BASE COUNT         1134 a         1136 c          730 g         1590 t
ORIGIN      
        1 gatcttcaaa ttccagtgta cagatagagt gtgttacccc ttcaaatgca tcctctaatg
       61 gatgagcaaa atcgtacatt ggataaatac accatttgtt tcctgtatta tgatgttcca
      121 ttcttgcgat acgataaaga attggatccc tcatgttgat atttccagat gtcatatcga
      181 tcttggcacg aagaactttg cttccatctg ggtattttcc ttccttcatc tcgcggaata
      241 actgtaagtt ctcttctggt gtacggtcac ggtatggact gttctttcca ggttctgtta
      301 aagttcctct gtattctctg atctgttcag cattcagatc acagacatat gcttttccct
      361 tcttgatcag gaattctgca cattcataca tcttgtcaaa gtagttagaa gcataataga
      421 tatgttcgtg ataatctgct cctaaccatt ctacgtcctc tttaatagaa tttacatatt
      481 cttccttctc ttttgttggg tttgtatcat caaaacggaa atggaaatct ccgccatatt
      541 cctgagccag tccatagttt aataagatag attttgcgtg tccgatatga agatatccgt
      601 ttggttctgg tggaaatctt gtaactacct tcttgcaatg cccttcttct atatcttttt
      661 ctatgatctg ttcgataaaa ttcttagaaa tcgtttcttc catgtcttcc tccatatagt
      721 tttttctatt ctgattataa tgtatcttct atacacttgc aatcatataa tttctgtatc
      781 atattgaact acagataatc cttctatata agatctaatg cctccatgac atttgaaact
      841 cctacaagtc tcatgtctgt ctggaccttg atctgatcta cattcgcctg aggcagaatg
      901 catcgtttaa atcctagttt ctgtgcttct ttgatccttg cttctccacc tgatacggct
      961 cttacttctc ctaccagacc aacttctcca aaaatgatcg tatcttctaa gatcggccgg
     1021 ttcttataac tcgatgcgat tgccataata ataccaagat cgatcgctgg ttcgccaagt
     1081 ttcatacctc cagccagatt cacatacgca tcacagccgc caagctgcag tccaactctc
     1141 ttctcaagca ctgccatcaa aagattcacc ctgttataat caattccaac tgctgttctt
     1201 ctcggcatct gaaagcttgt tggtgatacc aatgcctgaa tctcgatcag gatcggtctg
     1261 gtcccttcca tggaccatac aacaacggat cctgacgcat caaggggtct tccattaagc
     1321 atcgtctttg acggattctc cacttctaca agtccattgt tacacatctc aaacacaccg
     1381 atctcgttcg tggatccaaa acggtttttg acacctctta agattctgta tgccgcttct
     1441 ctttcccctt caaaataaag gaccgtatct accatatgct caagtgttct tggtcctgca
     1501 accactcctt ctttggtcac atgtcctacg ataaaaactg cgatattatt ctctttcgca
     1561 actctcatca tcgctgcagt cacttctctt acctgagaca cactgcctgc tgccgaggaa
     1621 agatcctcgc tgtacattgt ctgaatcgaa tcaatcaccg caaattctgg tttcgtcttt
     1681 aagatcgcat tcgtgatcgc atcaagatct gtctcacaat acagaagcat ctcttcttca
     1741 aatgttccga tcctctctgc tctcatcttg atctgatgca tagattcttc cccagaaata
     1801 tacaaaacct tacgtttgct attcgcaaga ttcctgcata cctgtaataa taacgtggat
     1861 ttcccgattc ccggatctcc tccgaccaac gatagggaac ctttgacgat cccgccgcct
     1921 aacacacggt ccagttcatg aatccctgtt ggaatccgct cttcattctc tactgttacc
     1981 tcggatatat tcataggaga ggcagaagtc tttaaagact tcgcccctcc ttttttcgtt
     2041 tctgtaactt tctcttctgt aaaagtattc cactgcttac aggcaggaca ctgccccatc
     2101 cacttggaag actcatgacc acactggctg caaaaataaa ctgtcttttg ttttgccatt
     2161 ggtgctcctt tatgttacta ctgtttcttt acacgaacag ctgctgctgt tccctcgctt
     2221 aactcaacac tcagagttaa ttttcctcca agatttgctt tcatcattcc tacattcgta
     2281 ttatcaataa aaactttata ttcctgtccg gcttctactt ctaaagtaac ctggacatca
     2341 gatgaagatt caatctcaaa tgcaactcca tctgttgttg caacaaagtt atgtactgct
     2401 gaacctggaa ctgattcaaa gatcagctgt ccgttcttgt ccagttttgt cattgtcttg
     2461 aaagtcttga tcttatagat atctccttca aactggatat ctcctttctt cgctttctgt
     2521 gctaatgtat agtcaccaaa gctaagtgtt ccattgtctt cgctgcggaa taattcagtt
     2581 accactgcca ttgtcttttc ctcctaatat atgtttcggt cgtcgtatgt atattataat
     2641 atatttcagt attatttact agttttcctt taccgaaaaa gtaagttttt tatctttttt
     2701 accaatcgtc atcgtctgtc cattcgtaag ggttccttct aagatcttat ctgccagtac
     2761 atcttcaatc tcattctgga tgatccttct taatggtctt gccccataat tctcatcata
     2821 tcccttatct gctaacagat ccatcgcacc agatgtgatc tttaatgtga tctgatgttc
     2881 gcttaataaa cgggctctca attcatccat aaataatttt gcgatcttct taacttcttc
     2941 ctttgtaagg acggcaaata cgatcgtctc atcaattcga tttaaaaact ctgggcgaaa
     3001 gatctgtttc acttcttcca taacaccctt tttcatacgt tcatgagtct tggctttatc
     3061 tgtatcatcc atagaaaatc ctaaatgttt tggtgcaatg atacggtttg cacctgcatt
     3121 ggaagtcata atgataacag tattcttaaa gtcgactttt cttccattgg aatctgtaat
     3181 atgtccgtca tctaatacct gaagcaggat attaaataca tcaggatgag ctttttcgat
     3241 ctcatcaaaa agaaggacag agtaaggatg tcttcgaatc tgttcactca gctgtccgcc
     3301 ttctccaaat ccaacatatc ctggaggaga tccgatgatc tttgatacac tgtgcttctc
     3361 catatattca gacatatcca cacggatcag atcttcttct cttccaaata aagcttttgc
     3421 taatgtcttt gacagctctg tcttaccaac tcctgttggc cctaaaaata agaatgatcc
     3481 gatcggacgg tttggactct taagaccgac tctgcttctc ttcacagctt tggcaactgc
     3541 attgaccgct tcatcttgcc cgatcacatg tttgtgaagt tctttctcta aatgtcttaa
     3601 tcgttccata tctccctttg taagctgtgt tactggaatc tgtgtccata aagaaacgac
     3661 ttctgcggca tcctctgttg tcaattctgg aatctcattt tgcttcttct gatttctctt
     3721 catcttacgt tccagagatt tctcaagttt gttcttctct ttcttaagct ctcttgcttc
     3781 tgtaatatct ccatcagaaa gcgctgcttc gatcttctct atgatctccc tgatctcctt
     3841 gcgttctttc ttaaaacctg catgacttcc aagctgttcc attcgtttcc ttgcacaggc
     3901 ttcatctaac agatcgatgg ctttatctgg cagaaaacga tcactgatat atcgatcaga
     3961 aagagaggcg atcgcctctg ccacatcttc tgataccagg atcctatgat aatcttcaaa
     4021 acgatgtttg atccctttta agatcgtgat cgtttcttct acggttggtt ctttcactct
     4081 gactggttgg aatcttcgaa ctaatgctgc atctttttca aaatattttg tatattcttc
     4141 actcgttgtc gcaccgatca gctgaaaatc tcctcttgcc aatgctggtt ttaagatatt
     4201 agatgcatcc atagctccct cagaacctcc agctccaatg atcgtatgaa tctcatcgat
     4261 aaacaaaata atagaatcat cctgtgttgc ttcggttacg atacgtttca ttcgttcttc
     4321 aaattctcca cggaatttcg ttccagcgat cactttcgta aggtctaagg acaaaatcct
     4381 cttattctta agaattcttg gcatattgcc ttcggcgatc ctttgtgcaa ggccttcaac
     4441 gatcgctgtt ttaccaaccc ctggatcacc gatcagacat ggattattct ttgtccttct
     4501 gcaaaggatc tgcatcagac gttccatctc actttcacgt ccgatcatcg gatcaagctt
     4561 cccaagcatt gctttctctg tcagatctgt
//
LOCUS       DAOMUE010000163         4586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5268_length_4586_cov_2.616862, whole genome shotgun sequence.
ACCESSION   DAOMUE010000163 DAOMUE010000000
VERSION     DAOMUE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5268_length_4586_cov_2.616862"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..870)
                     /locus_tag="PLC71_07580"
     CDS             complement(232..870)
                     /locus_tag="PLC71_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRM02539.1"
                     /translation="MKTSSKKIRSWSLMTLCPHHWKIGGVSLNSKLWTAKILAEQPEL
                     IKQVHKNYFKAGADIILFETVPSLKEAKVEAEIAEEYGYDYWISFSCLSENIICEGTP
                     IAECATTFAKGYPHLKMIGVNCTKPEYITGLIHKIKENCDIPIGVYPNSGEEYDAVKK
                     VWFGKQSALSFEQYAYNYMKSGASAVGGCCTTVAKHVEEVVRAKKRFSEEQK"
     assembly_gap    1051..1060
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1410..2267
                     /locus_tag="PLC71_07585"
     CDS             1410..2267
                     /locus_tag="PLC71_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM02540.1"
                     /translation="MKEKHYIKKVLNNNVVFSKNKDGKDIILTGLGLGFQKKKGEFVD
                     ENKIEHIFKIESDDINTKLSALLEQIPIDYFYIADCIKKLAEDDLNKELSDNIYVTLC
                     DHIYYAVERYHKGLIFQNQLVWEIRRFYPEEYQVAKKSIAIVNDALNISLSEDEASFI
                     ALHIINAEINGKELQSVIEMTKLIKDICNIVQYEFNIAFDEESLTYTRFILHLKFFSQ
                     RLLLHENVMEEANFLYDQVEQNMSEAFLCAKKISTYIKKSYEYEISKSEIVYLTIHIQ
                     RLLTQGQKL"
     gene            2350..4221
                     /locus_tag="PLC71_07590"
     CDS             2350..4221
                     /locus_tag="PLC71_07590"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HRM02541.1"
                     /translation="MTNKQLAEEILQKVGGKKNINSYSHCSTRLRFDLKDKNSIDKAA
                     LERTDGILQVMEVAGQTQVVIGSNVQYVYDELATLLPQGNTSSEKSDGKKKGVFGSAL
                     ELISSLFTPLIDVLIGAGILKGLLSILTATNLLTDASGTYQILNAAADSLYYFLPIVI
                     AITCSKKLKTNMFVSVTIAGALLYPNLTALYDAGTAITFLGIPVHLTAFKSSVFPIIF
                     AIVLLSFVEKGLKKILPDSIRSRIAPFFSLLIVVPVTIVIFGPLGSMLSDTIANFYMS
                     LFKFNPTIAGGFIGAIAQVLVIFGIHWGLFPIIFSNIEKFGFDTILAVFGPSIIAQSG
                     AAFGVWLKTKDKRLKQIASPAAIMGFFGISEPAIYGITLKYRKAFACAVIGGGIGGAI
                     AGSCGARAMAVAVAAVPTFPAYFGTGFTGFIIAYFGAFLISAVLTYFVGFNDSMIPES
                     ERYDAKGTVTPVASSIENNGAIAMKFYSPADGNVVPLTEVSDQAFSSEALGKGIAVKP
                     INGTIVSPIAGTVSAVYPTAHAYGIKGNNNEEILVHIGIDTVKLEGKYFTPNVKTNDI
                     VMPGDLLATIELDKIIEAGYDPTVMVIDTADSSKRNVTLLAKNTVHAKDDLVLIESI"
     gene            4234..>4586
                     /locus_tag="PLC71_07595"
     CDS             4234..>4586
                     /locus_tag="PLC71_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM02542.1"
                     /translation="MLQTTTMPLSKGSLHSCTFKRIPDETRFHVVDPNVIQKVESSGK
                     YILTYKEPDTTYLNIHELRKEMGSPNLDLCTEEIEEIPYLVSLSDRKIPITFFRIPNK
                     EKQSAIVFIHGGGYIG"
BASE COUNT         1437 a          774 c          779 g         1586 t
ORIGIN      
        1 tttgtattct ttacctcttt ttttatgatt cctatgcaga gtaacgtcat gacaaagatt
       61 acgcaacaga gcaacactcc ttctgttaac tgaattattt ttttcttttt atccatatgc
      121 tttccttctc tttttaaatt tccaatataa tcattataaa gaaaaaaatg acctctgaca
      181 atctatttag agattatcct gaggtcattt ttatgatttc atttttttca gttatttttg
      241 ttcttcagaa aaccgttttt tggctcttac gacttcctct acatgttttg ctacggttgt
      301 acaacatcct ccaactgcac ttgctccaga tttcatataa ttgtatgcgt attgttcaaa
      361 tgacagggct gactgtttgc caaaccagac tttcttcact gcatcgtact cttccccgct
      421 gtttgggtaa actccgatcg gaatatcaca gttttctttg atcttatgga tcaatcctgt
      481 gatatattct ggttttgtac agttgacacc gatcattttt aaatgaggat atccttttgc
      541 aaatgttgtt gcacattctg cgatcggtgt tccttcgcag ataatatttt cacttagaca
      601 tgagaaactg atccagtaat catatccata ttcttcagca atttctgcct caactttagc
      661 ttcttttaga gatggtactg tttcaaataa aatgatatct gctcctgctt tgaagtaatt
      721 tttgtgaacc tgtttgatca gttctggctg ttccgctaag atcttagctg tccacaactt
      781 ggaattcaga gagactcccc ctattttcca gtggtgtgga catagagtca tcaatgacca
      841 tgatcttatt ttctttgatg atgttttcaa tagaattctt catcataaaa catctccttt
      901 ttcttcgact gtttcataag atactttttc tggatgttct cctcttttta atgctggaat
      961 aaaacgatcc caaaaatcat tatatagtcg catgatctct tttcgtttgc tgtaaataaa
     1021 gatcaatgtg acaatgacat aaactgcact nnnnnnnnnn attttccagt ggtgtggaca
     1081 tagagtcatc aatgaccatg atcttatttt ctttgatgat attttcaata gaattaaaga
     1141 tcaatatgac aatgacataa actgcactta acaagtcggt tgccgcccat aaaagatcgg
     1201 ccttgatatt ataaaataat aagaaatatt gtattatgca tttcttacgt taatatttga
     1261 caacatttta tttttgtgct aaaatacgaa cataattgaa cgtggatcgt gactattata
     1321 tgaataggcg agactagaat ttatgataag gttattttta tacccttatt atattttctg
     1381 gtctcttttt tattttggag gatacaagta tgaaagaaaa acactacata aagaaagtgc
     1441 tcaataataa tgtagtattt tcgaaaaaca aagatggaaa agatattatt ttaactggct
     1501 taggattagg ctttcaaaag aaaaaaggag aatttgttga tgaaaataaa attgagcata
     1561 tttttaaaat tgaaagcgat gatatcaaca caaaattatc cgcattatta gaacagattc
     1621 ctattgatta cttctatatt gcagattgca tcaaaaaact agccgaagat gatcttaaca
     1681 aagagttaag tgataatatc tatgttacac tttgtgatca tatttattat gccgttgaga
     1741 gatatcataa gggcttgatc ttccaaaacc agctggtctg ggaaattagg cgtttttatc
     1801 ctgaagagta tcaggttgcc aaaaaatcaa ttgctattgt caatgatgct ttaaatattt
     1861 cactttctga agatgaagca agctttattg cgttacatat cattaatgct gagatcaacg
     1921 gaaaagaact tcaatctgtt attgaaatga ccaaactaat caaagatatt tgtaacatcg
     1981 ttcaatatga atttaacata gcatttgatg aggaatcttt aacttatacc cggtttattc
     2041 ttcatttaaa attcttttca caacgattac tgcttcatga aaatgttatg gaagaagcta
     2101 attttcttta tgaccaagta gagcaaaata tgtcagaagc ttttttatgt gctaagaaaa
     2161 tctctactta tattaaaaaa agctatgaat acgaaatatc aaaaagtgaa attgtttatc
     2221 tgacaatcca tattcaacgc cttttaacac aagggcaaaa attataagtt tcattatgta
     2281 cctaagattg tgactacttc gagtaggcaa gacttaagat aaattttaaa tcttaaggag
     2341 gaaaagaaaa tgactaacaa acaactcgca gaagaaattt tgcaaaaagt aggaggtaag
     2401 aaaaatatta attcttactc tcactgctcc acaaggcttc gctttgatct taaagataaa
     2461 aattctattg acaaagctgc cttagaaaga accgatggca tattacaggt aatggaagtt
     2521 gccgggcaaa cacaggttgt aattggttca aatgttcaat atgtatacga tgaacttgcc
     2581 acattactcc cacaaggtaa tacatcttct gaaaaatcag acggtaaaaa aaagggagtc
     2641 ttcggatctg ctctagaact aatctcaagc ttatttactc cgctgattga tgtgttaatc
     2701 ggtgcaggta tactaaaggg gcttttaagt atccttacag ctaccaattt attaactgac
     2761 gcaagtggta cctatcagat tttaaatgca gccgctgaca gtttgtatta ttttttacca
     2821 attgttattg caatcacatg ctctaagaaa ttaaaaacaa atatgtttgt ttctgtaaca
     2881 attgctggtg cgctattata cccaaattta actgcattat atgatgcagg aactgcaatt
     2941 acttttttag gaattcccgt tcatttgact gcatttaaga gcagtgtatt tccaattatt
     3001 tttgctattg tgttgctatc ttttgtagag aaaggactaa aaaagattct tcctgattct
     3061 attagaagcc gaattgctcc ttttttctca ttactgatcg tagttccagt aacaattgtt
     3121 atttttggac ctttaggatc tatgttaagt gatacaatcg ctaactttta tatgagtctt
     3181 ttcaaattta atccaacaat tgctggtggt ttcattggtg caatcgctca ggtattagta
     3241 atttttggta ttcactgggg actgttccct attatcttct ccaatatcga gaaatttgga
     3301 tttgatacaa ttttagctgt ttttggacca agtatcatcg ctcagtctgg tgctgcattt
     3361 ggtgtatggt taaagacaaa agataaacgt ttaaaacaga ttgcatcccc tgcagctatt
     3421 atgggattct ttggtatttc agaacctgcg atttatggaa tcactttaaa atatcgtaaa
     3481 gcatttgcat gtgctgttat cggtggtggt atcggtggtg cgattgctgg gtcttgcggt
     3541 gctcgtgcta tggctgtcgc tgttgctgca gttccaacct tccctgctta ctttggaacg
     3601 ggatttactg gatttatcat tgcttacttt ggagcattct taatttcagc tgtcttaact
     3661 tattttgtag gatttaatga ttccatgatc cctgaatctg aacgttatga tgcaaaagga
     3721 actgttacac ctgttgcatc ttctattgaa aacaatgggg ctattgcaat gaaattctac
     3781 agccctgctg atggaaacgt tgtaccttta acagaagtat ctgatcaggc cttttcttca
     3841 gaagctttag gaaaaggaat tgctgtaaaa ccaatcaatg gaacaattgt ttcaccgatt
     3901 gctggaaccg tttctgctgt atatccaaca gctcatgcat atggaattaa aggaaataat
     3961 aacgaagaaa tcctggttca catcggaatt gatactgtaa aattagaagg taaatatttt
     4021 acaccaaatg taaaaacaaa tgatattgta atgcctggag atcttctggc aacgattgaa
     4081 ttagataaga tcattgaagc tggttatgat ccaaccgtta tggttattga tacagctgac
     4141 agttccaaac gaaatgttac attattagca aaaaatacgg ttcatgcaaa agacgatctt
     4201 gtcctaattg aatctatata aggaagtgat atcatgttac aaacaacaac gatgccttta
     4261 tcgaaaggct ccctacattc atgtactttt aaaagaattc ctgatgaaac acgttttcac
     4321 gttgttgatc ctaacgtgat ccaaaaggta gaatcttctg gaaaatatat tcttacatat
     4381 aaagaacctg atacaaccta tttgaatatt catgaattaa gaaaagagat gggatctcca
     4441 aaccttgatc tgtgtactga ggaaatcgaa gaaattccat atcttgtttc attatctgac
     4501 cggaaaattc cgattacttt tttccgaatt ccaaataagg aaaaacaatc tgccatcgtg
     4561 tttattcatg gtggcgggta tattgg
//
LOCUS       DAOMUE010000164         4566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5297_length_4566_cov_2.324097, whole genome shotgun sequence.
ACCESSION   DAOMUE010000164 DAOMUE010000000
VERSION     DAOMUE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5297_length_4566_cov_2.324097"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /gene="hslO"
                     /locus_tag="PLC71_07600"
     CDS             complement(<1..705)
                     /gene="hslO"
                     /locus_tag="PLC71_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRM02543.1"
                     /translation="MNDYIIRGTAANDQVRFFAAYTKDVVETARQKHNTSPVATAALG
                     RLLTAGAMMGSMCKNDSDVITLQIQCSGPIGGLTVTADSKANVKGYVNNPDVMLPPSK
                     EGKLDVGKALDLGVLTVIKDIGLKEPYSGQTHLVSGEIAEDLTYYFAASEQIPTSVAL
                     GVLMNKDNTVRQAGGFIIQMMPYAEEETISKLEKKIAEFKSVTYALEHEHTPEKMMED
                     LLGDMDMKIYEKVPTQF"
     gene            complement(1006..1404)
                     /locus_tag="PLC71_07605"
                     /pseudo
     CDS             complement(1006..1404)
                     /locus_tag="PLC71_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(1456..2982)
                     /gene="cls"
                     /locus_tag="PLC71_07610"
     CDS             complement(1456..2982)
                     /gene="cls"
                     /locus_tag="PLC71_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HRM02544.1"
                     /translation="MKIAEHLTKPISKILKFLLRRLVFVTVAIILQMIWMTYMVVVIG
                     EYSKAIEWVFRIFSITVVIYIANKDDNPAYKLAWTIPILLFPIFGGFLYLVLGDKQPA
                     KKLRMELEQSIEDTEFLLGQDYGVMERLEQEDYQVAGQAKYIDQYGGYPIYENSDAKY
                     YPSGEAMFKELIEDLKKAKHYIFMEFFIVSRGYMWDTILEVLKDRVNDGVEVRFMYDD
                     VGCVDLLPYKYYKELERFGIMAMPFNPIKPFVSTAWNNRDHRKVVVIDGHTAYTGGLN
                     LSDEYINKVERFGYWKDAGLKVTGDAVWNFTVMFLQVWNAIRKTDEGAFKGKGFIQPY
                     ADNPLDHEIVGENVYLNIINTANHYVYIYTPYLIIDNEMTTALCLAAKRGVDIKIVTP
                     GIPDKKLVFLLTQSYYKQLVEAGIHIYEYTPGFIHAKCFASDDKVATVGTINMDYRSL
                     YLHFENGVFMYKNDAVMQLKHDMEKTFEISQRITKAMCQGNLRKTLTQSVLRLLAPLL
                     "
     gene            complement(3052..3609)
                     /locus_tag="PLC71_07615"
     CDS             complement(3052..3609)
                     /locus_tag="PLC71_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRM02545.1"
                     /translation="MNKPCFIAVTNRNLCKRPFFDVIEDLSKKDVKTIVLREKDLSEE
                     EYYEIAEKCKEICDRNGASLTIHNFIDVARRLGIKKIHLPYPVFLKEAGNLSDFESVS
                     TSIHKPEEAIKAQKLGVDFVFAGHVFVTDCKKGLPPRGLEFLTDVVNAVKIPVYGIGG
                     INEENITKIMECGAAGGCMMSGFMK"
     gene            complement(3611..3829)
                     /locus_tag="PLC71_07620"
     CDS             complement(3611..3829)
                     /locus_tag="PLC71_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HRM02546.1"
                     /translation="MPKEKDFIEKTFEEMNEMERLKYETAAELGLLDQLKKSGWKSLS
                     ASETGRLGGIMNRKIRKQKESQENTKGS"
     gene            complement(3889..>4566)
                     /locus_tag="PLC71_07625"
     CDS             complement(3889..>4566)
                     /locus_tag="PLC71_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRM02547.1"
                     /translation="NEAETKGRLKYSVSEIKRMSQAADTEEVMYSSVSMEKEKRIPSF
                     VKKTEKISAASKGTIIHKVFELLDFSKDYTMKSLNEDIERWISLGKLKKEYEKVIYRK
                     EILALTRSELGKRMKEAAQKKLLYKERQFVTGIPFSDMNKDAKTDDFVVVQGIIDVYF
                     EEEDKIILVDYKTDRVREGEEDILIRRYRAQMESYQQALEKITGKCVKEIYIYSVTLQ
                     KTIPVHV"
BASE COUNT         1254 a         1092 c          613 g         1607 t
ORIGIN      
        1 aaattgtgtt ggaactttct catagatctt catatccata tcacctaaga gatcctccat
       61 catcttctct ggtgtatgtt catgctctaa tgcataagtt acggatttga attctgcgat
      121 cttcttctct aacttactga tcgtctcttc ctctgcatat ggcatcatct gaatgataaa
      181 tccacctgcc tgacgaaccg tgttatcttt gttcattaaa actccaagtg ctacagatgt
      241 tggaatctgc tcacttgccg caaaataata tgttaaatct tctgcaatct ctccagatac
      301 taaatgtgtc tgtcctgaat aaggctcttt taatccaata tccttaatca ctgtcaaaac
      361 acctagatct aatgctttcc caacatctaa ttttccttct ttacttggag gaagcatcac
      421 atctggatta tttacatacc ctttcacatt tgccttagaa tcagctgtta ctgttaaccc
      481 gccgattggt ccactgcact ggatctgtaa tgtgatcaca tcactgtcat tcttacacat
      541 acttcccatc atagctccgg ctgttaataa tcttccaagt gccgccgttg ctaccggact
      601 tgtattatgt ttctgtcttg ctgtctccac gacatctttt gtatatgcag caaaaaaacg
      661 aacctggtca tttgctgctg ttcctctgat aatataatcg ttcatctttt ttcctttcaa
      721 tttgcaatat aatctttgtg tatcttcttt aacttcatcc attgtctctg catccagaac
      781 acgcacggat gaaaatcccg cttcataaag aaagcttaca acctcttctt gttcgtaagc
      841 tttctgtaaa tgtaattctt cgtacttgtt atatgtacct tgctcattct gaataaacag
      901 gctcaactga tattcattga tacgttcttc ttcatcataa aaattatccc agataaagct
      961 cgcatcctca cggtcttctg caaatgtatt gcacgcaagg atctctttat acttataatg
     1021 actgttcata tcaaatacaa acacaccatc ttcttttaaa aattctctga ctcttctgaa
     1081 tgcttctttt aaatcttctt cctctagaat ataattcata caatcgcaga tactaaccgc
     1141 tgcatctacc tgctgcggaa gctctaatga caccatgtcc tgcaatacat ataaaatatg
     1201 atcatcctct tcactagccg cgatctccag catatccata gaattatcaa cagccgtaac
     1261 ctgataacct tctcttgcaa gctttcgtgc catctttcct gtcccacatc caagatctaa
     1321 tacctgtgca tctttatcaa tcttatattc tttgaatata tttaaaatat ttaaaagcca
     1381 ttcatcatat ggggtctgat ccataaattc atcatacacc cttgcaaaat ctctgtaact
     1441 ttccatctta tcctcttata gtaatggcgc cagtaatcgt aagacactct gtgtcaatgt
     1501 ttttcttaaa tttccttgac acatcgcttt tgtgatcctc tggctgatct caaatgtttt
     1561 ctccatatca tgttttaact gcataactgc atcattctta tacataaaaa caccattttc
     1621 aaaatgcaga taaaggcttc gataatccat gttgatcgtt ccaactgttg ccaccttatc
     1681 atcacttgca aaacactttg catgaataaa cccaggagta tattcataaa tatgaattcc
     1741 agcttccact aactgcttat aataagattg tgtcaataag aatactaact tcttatctgg
     1801 aattccagga gttacgatct ttatatcaac tcctcttttg gcagcaaggc aaagagctgt
     1861 tgtcatctcg ttgtcaatga tcagatatgg ggtgtaaata taaacataat gatttgcagt
     1921 attaatgata tttaaatata cgttctctcc aacgatctca tgatctaatg gattatccgc
     1981 ataaggttgt ataaatccct ttcctttaaa ggctccttca tctgtcttgc ggatcgcatt
     2041 ccatacctgt aaaaacataa cagtgaaatt ccacactgca tctcccgtca ccttaagtcc
     2101 ggcatctttc caatatccaa aacgttcaac cttattaata tattcatcag ataaattcaa
     2161 tccaccagta tatgctgtat gcccatcgat cacaactact tttctatgat ctctgttatt
     2221 ccatgccgtt gaaacaaatg gtttgatcgg attaaatggc atcgccatga tcccaaaccg
     2281 ctcaagctct ttgtaatatt tataagggag cagatccaca catcctacat catcgtacat
     2341 aaaacgtact tcaacaccat cattgactct gtctttcaga acttccaaga tcgtatccca
     2401 catatatcct cttgaaacaa taaaaaattc cataaagata taatgctttg ccttcttaag
     2461 gtcctcgatc agttctttaa acattgcctc tcccgatgga taatacttcg catcggaatt
     2521 ctcgtaaatc ggatatccac catactgatc tatatacttt gcctgtcctg cgacctgata
     2581 atcttcctgt tccagtcgtt ccatcactcc ataatcctgt ccaagcagaa attctgtatc
     2641 ctcgatcgac tgttcaagtt ccatacggag cttcttcgct ggctgcttat ctcccaggac
     2701 cagatataag aatccgccaa aaatcggaaa caaaaggatt ggtatcgtcc atgcaagttt
     2761 atacgccgga ttatcatctt tattcgcaat atagatcaca acagtaatgc tgaatatgcg
     2821 aaatacccat tcgatcgctt tggaatattc tccgatcacc acaaccatat aggtcatcca
     2881 gatcatctgt aaaatgatcg caacggtcac gaataccagt cttcgaagta aaaatttaag
     2941 aatttttgaa attggttttg ttaaatgttc tgctatcttc atcttacgtc cttaccttct
     3001 tcaaaagcta cttcttttca tcttaacata gataccattc aatttcaatt tttatttcat
     3061 gaatcctgac atcatgcagc ctcccgctgc cccgcattcc atgatctttg taatattttc
     3121 ttcattaatt ccaccaattc catacaccgg aatcttgacc gcatttacta catccgtcaa
     3181 aaattcaagt ccccttggcg gcagaccttt tttacaatct gtcacaaaga catgtcctgc
     3241 aaacacaaaa tccaccccca gtttctgtgc ttttatcgcc tcttctggct tatggatcga
     3301 tgtgctgaca gattcaaaat cagacagatt ccccgcttct tttaaaaata ccggatatgg
     3361 aagatgaatc ttcttgattc caagtcttct tgccacatcg ataaaattat gaatggttaa
     3421 ggaagcaccg tttctgtcac agatttcctt acatttctct gcgatctcat agtactcttc
     3481 ttctgacaga tccttttctc ttaatacgat cgttttcaca tctttctttg acagatcctc
     3541 gatcacatca aaaaatggtc gtttacatag atttctattt gtcactgcga taaaacatgg
     3601 tttgttcatt ttaacttcct ttcgtatttt cctgactttc cttctgcttt cttatcttac
     3661 ggttcatgat cccgcctaat cttcctgttt cactggctga taatgacttc catccactct
     3721 tctttagttg atcaagcaga ccaagttctg cagcagtttc atattttaac cgttccatct
     3781 cattcatttc ttcgaatgtc ttttctataa aatccttttc ctttggcatg atcttcttcc
     3841 tttctgattt gtcattttct attcttagaa tatgacaaat cagaaatttc atacatgcac
     3901 gggaattgtt ttttgcaggg ttacagaata aatgtaaatt tcttttacac actttcctgt
     3961 gatcttttcc aatgcttgtt ggtaagattc catctgtgca cgataacgtc tgatcaaaat
     4021 atcctcttcc ccttctctga cccggtctgt tttataatct accaaaataa ttttatcttc
     4081 ttcctcaaaa tatacatcaa taattccctg aacaacaaca aaatcatccg tttttgcatc
     4141 tttgttcatg tcagaaaatg gaattcccgt cacgaactgc cgttctttat acagcagttt
     4201 cttctgtgct gcctctttca tcctcttccc aagttctgat ctcgtcagag cgagaatttc
     4261 ttttcgatag attacttttt catattcttt ttttaatttt ccaagagaga tccatctttc
     4321 gatatcttcg tttaaacttt tcattgtata gtctttggaa aaatctagta attcaaaaac
     4381 tttgtgaatg atcgtacctt tggatgccgc tgaaatcttt tcagttttct tgacaaatga
     4441 tggtatcctt ttttctttct ccatagaaac agatgaatac atcacttcct cagtatctgc
     4501 tgcctgactc atacgtttga tctctgatac agaatatttt aaacgacctt ttgtctctgc
     4561 ctcatt
//
LOCUS       DAOMUE010000165         4532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5359_length_4532_cov_2.052044, whole genome shotgun sequence.
ACCESSION   DAOMUE010000165 DAOMUE010000000
VERSION     DAOMUE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5359_length_4532_cov_2.052044"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PLC71_07630"
     CDS             <1..305
                     /locus_tag="PLC71_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRM02548.1"
                     /translation="DLKKVFTNKRIYLVTFIRLVIEPIIALAVIILLGMKNWHPQAEN
                     IILITYMAAITPCASTITQMCQVYGNDSKYASAINVMTTLLAVVTMPVFVYFYMNL"
     gene            complement(448..1914)
                     /locus_tag="PLC71_07635"
     CDS             complement(448..1914)
                     /locus_tag="PLC71_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM02549.1"
                     /translation="MNQTFTFADRLKSAMKQKHMKQVDLIHAAETAGVKVGKSHISQY
                     VSGKTVPRAEILHFLSETLDVDADWLSGNGGNAVNLETSPRRTFSKSTKLDNVLYDVR
                     GPVVDEANRMERNGTHILKLNIGNPAPFGFRTPDEVIYDMRHQLADCQGYSASQGLFA
                     ARKAIMQYAQLKKIPNVDIDDIYTGNGVSELINLSMSALLDDGDEILIPSPDYPLWTA
                     CATLAGGKAVHYICDEQSEWYPDMEDIKRKVNSHTKAIVLINPNNPTGALYPREVLQQ
                     IVDIAREHQLMIFSDEIYDRLVMDGEEHVSIASLAPDLFCVTFSGLSKSHMIAGFRIG
                     WMILSGNKAIARDYIEGLNMLSNMRLCSNVPAQAVVQTALGGHQSVNDYIIPGGRIYE
                     QREFIYNALCDIPGISAVKPKAAFYIFPKIDTKKFNITNDEQFALDLLREKKILIVHG
                     SGFNWKDPDHFRVVYLPRVEVLEDASVKLRDFLEYYRQ"
     gene            2393..>2932
                     /locus_tag="PLC71_07640"
     CDS             2393..>2932
                     /locus_tag="PLC71_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRM02550.1"
                     /translation="MEATRLQMQNFLIFLLDLVGVTVGYFAAVWLRFGNIETGYTVMG
                     NDTYYRWLIAIVVLMMIYFLFRPNRGFFKRKFIEEMRMNLQTVVLMAAGMAMVAFLIQ
                     DAEDYSRFIYIFTSGFGFVWMQITHRAYRKYWLSRRKDHSYARKMMIITTSDHAEEVI
                     GNIMEEKNWDLWITSVAVVD"
     assembly_gap    2933..2942
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2943..3825
                     /locus_tag="PLC71_07645"
     CDS             <2943..3825
                     /locus_tag="PLC71_07645"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRM02551.1"
                     /translation="MTGWLINEIPVVAHSYRSIFEYAMRSVVDEVFLYIPMDDEFPIA
                     MTVQNFEDMGIKTNLNISQFQINENLERSLEKVGPYESVSFSGHNVSFVMLMMKRAMD
                     IAGGLVGMLITAIAVIIVGPLVKLESPGPLFFSQKRVGKNGRIFKIYKIRSMYQDAEE
                     RKKELMAQNEMDGLMFKMKDDPRITKVGKFIRKTSIDELPQFWNVLKGDMSLVGTRPP
                     TVDEFEQYSAYHKKRLCQKPGLTGVWQVSGRSTITDFEEIVQMDVDYIDHWSIWRDIG
                     ILFKTVWLVVCGDDGAQ"
     gene            3868..>4532
                     /locus_tag="PLC71_07650"
     CDS             3868..>4532
                     /locus_tag="PLC71_07650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRM02552.1"
                     /translation="MSEERKIRIVHIAQSPGGVERYLRSLLKYSNREKYEIILIVSNQ
                     YKRKNYVNLVDAFENVDMIRSIDPKSDLNAIKAIRKLLKKYKPDIVYCHSSKAGALGR
                     IANMGLKNKCVYNPHGWAFNMRGNKLKKLIYVGIEKVLAPFCSTIICISGAEKRSALK
                     YRICGKKKLKVIYNGIDFDEYNMDYHMNTRELLEIPEDAFVVGMVGRLAEQKAPDVFV
                     KAA"
BASE COUNT         1476 a          780 c          983 g         1283 t
ORIGIN      
        1 ttgatctaaa gaaagtattt acaaacaaaa gaatctacct ggtcacattc atccgattag
       61 tgatcgaacc gatcatagca cttgcagtca ttattctgtt aggaatgaaa aactggcacc
      121 cacaggcaga aaacatcatc ctgatcacat acatggcagc gatcacacca tgcgcctcaa
      181 cgatcacaca gatgtgtcag gtgtacggaa acgattcgaa atatgcaagc gcgatcaatg
      241 taatgacgac attgctggcg gtggttacta tgccggtgtt tgtttatttt tatatgaatc
      301 tgtaatgtaa aataattaca agtaaaatat tgacaaaaat gataatgata gtataataag
      361 agataataaa aaagagaact tacatacaag cgggtaagtt ctctttttga ttgtacattc
      421 ttcaaaaatt tataatggag taagatttta ctgtctataa tactccaaga aatccctcag
      481 cttcacagaa gcatcctcaa gcacttccac acgaggcaga taaactactc tgaaatgatc
      541 cggatccttc cagttaaatc cacttccatg aacgatcagg atctttttct cacgaagcag
      601 atccagagca aactgctcat catttgtaat gttaaacttc ttcgtatcaa tcttcgggaa
      661 gatataaaaa gcagcttttg gtttcactgc actgatacca gggatatcac acaacgcatt
      721 ataaataaac tctctctgtt cataaatacg tccaccagga atgatgtaat cattgacact
      781 ctgatggccg ccaagtgctg tctggacaac tgcctgtgca ggaacattgg aacaaagtcg
      841 catatttgat aacatattta aaccttcaat ataatccctc gcgatcgcct tattaccact
      901 taagatcatc catccgatac ggaaaccagc gatcatatga gacttggata aaccagagaa
      961 tgtcacacag aacagatctg gagctaagga agcaatagat acatgttctt ctccatccat
     1021 caccagacgg tcataaatct catcagagaa gatcatcaac tgatgttctc tcgcgatatc
     1081 aacgatctgc tgtaagactt ctcttggata aagagccccc gtagggttgt taggattgat
     1141 cagaacgatc gcttttgtat gggaatttac ttttcgcttg atatcttcca tatcaggata
     1201 ccactcagac tgttcatcac agatataatg aactgctttc ccaccagcaa gcgttgcaca
     1261 agccgtccat aaaggataat ccggagaagg gatcaaaatc tcatccccat catctaataa
     1321 agcagacatt gacagattga tcagctcact gacaccattt ccagtataaa tgtcgtcgat
     1381 atcaacattt gggatcttct taagctgcgc atactgcatg atcgcctttc ttgcagcaaa
     1441 taatccctga gaagcagaat atccctggca gtcagctaac tgatgtctca tatcatagat
     1501 gacttcatcc ggggttctga aaccaaatgg agcaggattc ccgatattta atttcaggat
     1561 atgagttcca ttacgttcca tgcggttcgc ttcatctacg acaggaccac gtacatcgta
     1621 aagtacatta tctaatttgg tagatttgct aaatgttctc cttggagaag tttccagatt
     1681 cacagcattc cctccatttc cagaaagcca gtcagcatcg acatcaagag tttcagataa
     1741 gaaatgaaga atctctgcac gaggaaccgt cttgccgctg acatactggc tgatatgact
     1801 tttcccgacc ttgactccgg cagtctctgc tgcatgaata agatcaacct gtttcatatg
     1861 tttttgtttc attgcagact ttaagcgatc tgcaaaggta aatgtctgat tcataacacc
     1921 ctccgataaa agttcaaaat aaaattcaaa gttcaaatga ttttgtttaa tatagcacaa
     1981 aaacaagaaa agttcaatga aaagttcaaa ataaaagcca aaagttcaaa gaaacaatgt
     2041 gcaaaataag atgagagtgt caaaagtaaa ttttgccact ctctgatgta cccggattgt
     2101 tccattgcta cgcaattcca caatcctcga acacctcgaa cttccataaa atggcgtgtt
     2161 cacaccattt tattccgttc tcgttgttcg gcgtgtcaat aagtataaat tgcttatttg
     2221 tgcaagcaca aacgcaattt atacttttga cacttacatc aaaataagaa aaaaattaaa
     2281 gttcgacaaa aagtacaaag atttgtattg aaatttagaa aatacatgct atactttgaa
     2341 aagtatagat aattaacaca aagtgtataa ataaatgggg atgaaataga atatggaagc
     2401 aacaagatta cagatgcaga acttcctaat ctttttatta gatctggtgg gagttactgt
     2461 tggttatttt gcagcagtat ggctgcggtt tggaaatatt gaaacagggt ataccgtgat
     2521 ggggaacgat acatattatc gttggctcat tgcgatcgta gtgcttatga tgatctactt
     2581 tctgtttcgt ccaaatcgtg gtttttttaa gcgcaaattt atagaagaaa tgagaatgaa
     2641 tctgcagacg gtcgtcctga tggcagcagg aatggcgatg gtcgcatttc tgattcagga
     2701 tgcagaagat tactccagat ttatttatat ctttacttct ggatttggat ttgtatggat
     2761 gcagatcaca catcgagctt atcggaagta ttggctatca agaagaaagg atcattctta
     2821 tgcaagaaag atgatgatca tcacaacgag tgatcatgca gaagaagtga tcggaaacat
     2881 catggaagag aagaactggg acctgtggat cacaagtgtt gcagtcgtgg acnnnnnnnn
     2941 nncatgacag gatggcttat caacgagatt ccggttgtag cacattcata tcgaagtatt
     3001 tttgaatatg cgatgcggtc ggttgtggat gaagtattct tatatatacc gatggatgac
     3061 gaatttccaa tcgcgatgac agtacagaac tttgaggaca tgggaattaa gacgaatctt
     3121 aacatttccc agttccagat caatgagaat ctggaacgat cactggagaa ggttggacct
     3181 tatgagagtg taagtttctc tggtcataat gtatcatttg tcatgctgat gatgaagaga
     3241 gcaatggata ttgccggggg attggttgga atgctgatca cagcgatcgc agtgatcatt
     3301 gtcggaccac tggtgaagct agaatcacca gggccgctgt tcttctcaca gaagagagtt
     3361 gggaagaatg gaagaatctt caagatctat aagatccgtt cgatgtatca ggatgcggaa
     3421 gagcgcaaga aagagcttat ggcacagaat gagatggacg gcttgatgtt taagatgaaa
     3481 gacgatccga gaattaccaa ggttggaaag tttatcagga agactagtat tgatgaactt
     3541 ccacagtttt ggaatgtact gaagggggat atgagtctgg tagggacaag accacctaca
     3601 gtggatgagt ttgaacagta tagtgcttac cataagaaga gactttgcca gaagcctggg
     3661 ctgactgggg tttggcaggt gagtggaaga agtacgatca cggactttga ggagattgtt
     3721 cagatggatg ttgattatat cgatcattgg tctatctgga gggatattgg gatattgttt
     3781 aagacagtgt ggcttgttgt ttgtggggat gatggggcac aataggagta gaagaagagt
     3841 tgatgatgga ttaagaaggg aaattcgatg tcagaagaga gaaagataag aattgttcat
     3901 attgcacaat ctcccggagg tgtggaacga tatttaaggt ccttacttaa atatagtaat
     3961 agggaaaaat atgagattat tctgattgtt tcaaatcaat ataagaggaa aaactatgta
     4021 aatttggtag atgcttttga aaatgtggat atgatcagaa gtattgatcc aaaaagtgat
     4081 ttaaatgcga ttaaggcaat acgtaagttg ttgaagaaat ataagccgga tattgtttat
     4141 tgccattcta gtaaggcagg agcattggga cgaattgcca acatgggatt aaaaaataag
     4201 tgtgtgtata atccacatgg atgggcattt aatatgaggg gaaataagtt aaagaaatta
     4261 atatatgttg gaattgaaaa ggtgttggca ccattttgtt caacgattat ttgtatttcg
     4321 ggggctgaga aaagatcagc attaaaatat aggatttgtg gtaaaaaaaa gcttaaggta
     4381 atttataatg ggattgattt cgatgagtat aatatggatt atcatatgaa tacgagagaa
     4441 cttttagaaa ttccagaaga tgcatttgtt gttggtatgg tgggaagact ggcagaacag
     4501 aaggcaccag atgtgtttgt aaaggcagcg aa
//
LOCUS       DAOMUE010000166         4529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5363_length_4529_cov_2.248100, whole genome shotgun sequence.
ACCESSION   DAOMUE010000166 DAOMUE010000000
VERSION     DAOMUE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5363_length_4529_cov_2.248100"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /gene="lon"
                     /locus_tag="PLC71_07655"
     CDS             <1..1373
                     /gene="lon"
                     /locus_tag="PLC71_07655"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRM02553.1"
                     /translation="QVLDRDHYGMEKVKERVLEYLAARAMSGKKDAAILCLVGPPGTG
                     KTSIARSIAKATNKKYIRLSLGGVRDESEIRGHRKTYVGAMPGRIVEALKKVKVRNPL
                     MLLDEIDKTGKDQRGDTASALLEVLDPEQNEHFTDHYLEVALNLSDVLFVATANDLST
                     IPRPLLDRMEIIEVSSYTDNEKYHIANDYLVKKQMEANGLNDTQIHWKEDALRFLITD
                     YTREAGVRNLERRIGQVSRKVTRDIYQKKHRKVTITKKVIKDYLGRPVYEWEKDTLRD
                     HVGIVHGLAWTAVGGVTLKIEVNVMPGKGAIQVTGSLGNVMKESAQAAISYIRSQSRK
                     YKIKQDFFEKHDIHIHIPEGAVPKDGPSAGITMTTAVLSAITGNKVCGNLAMTGEVTI
                     RGDVLMIGGLKEKLLAAKRLGITKVLVPKSNEKDVKEFEEEVVEGLEIVYVKTMTQVI
                     KEAFVH"
     gene            1403..2677
                     /locus_tag="PLC71_07660"
     CDS             1403..2677
                     /locus_tag="PLC71_07660"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRM02554.1"
                     /translation="MNYEEAMNFIQNTNKFGSVLGLDNIKELLERLGNPQDQLKVVHI
                     AGTNGKGSTLAFLAGIFRESGYRAGRYVSPASFCYEERFRINEENISKEDLCFYMEKI
                     KKISEEMVRDGMSHPTMFEIETALSFLYFLDKKVDVVLLETGMGGRLDATNVVKKPIA
                     VIIASIGMDHMQFLGDTIEKIAAEKAGIIKEGCPVISYNNQESVNEVIKEKAKEMHCK
                     VTFVNSEGIRVLSESLNGESFSYRSSDGRWYEKIEIPLLGRHQINNAALALEALNTIR
                     NYYCISEFQTEDGLRKTIWRGRIEILEKDPLVICDGAHNPDGAKSLLNFLQNNFTNRR
                     LIYIMGVLSDKDYEQMIQILTPMAAKVYTVAPDNARALSSKDLAECVRKYHHDVEERQ
                     RLKECLEEVKQQADKEDVIIICGTLSFQNELK"
     gene            2690..3169
                     /locus_tag="PLC71_07665"
     CDS             2690..3169
                     /locus_tag="PLC71_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM02555.1"
                     /translation="MERFQKIVWHEKYKETYERLQQLEIDRKFCGHDIEHFLSVARIS
                     YLMILEKRLPVSKDIIYATALLHDLGRADQYEKGISHEEAGAILAEEILTDCGYTREE
                     RKFMTDTILRHRDMKEESESFASIFYRADKLSRDCIHCKARQECYWPEEKKNNKYIY"
     gene            3180..3894
                     /gene="folP"
                     /locus_tag="PLC71_07670"
                     /pseudo
     CDS             3180..3894
                     /gene="folP"
                     /locus_tag="PLC71_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392622.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
     assembly_gap    3895..3904
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3973..>4529
                     /gene="folB"
                     /locus_tag="PLC71_07675"
     CDS             3973..>4529
                     /gene="folB"
                     /locus_tag="PLC71_07675"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRM02556.1"
                     /translation="MDQITIKDLEVYANHGLYKEEKALGQKFLVSAILSLDTKLAGVS
                     DQMDYSVDYGKVCHRIKEILTENDFNLIECVAETVAKKLLLEFSLIRKLEIEVKKPWA
                     PIGLPLDYVSVKIKRGWHRAYLGVGSNMGDRMEYINQAINAIEAQDDTRVVHVSSLIE
                     TKPYGGVVQDDFLNGCIAIDTLKEP"
BASE COUNT         1606 a          665 c         1110 g         1138 t
ORIGIN      
        1 aacaggtgct cgatcgagat cattatggaa tggaaaaagt caaggaacgt gttttggaat
       61 atttagcagc acgtgctatg agtggaaaga aagatgcagc gatcctttgt cttgtaggac
      121 ctccaggaac aggaaagaca tctattgcca gatcaattgc gaaagcaacg aacaaaaaat
      181 atattcgttt atctcttggc ggcgtcaggg atgaatcaga gatccgtgga cataggaaaa
      241 cttatgttgg agcgatgcca ggacgtattg ttgaagcatt aaagaaggta aaagttagaa
      301 atccgttaat gttattggat gagatcgata agacagggaa agaccagaga ggagataccg
      361 cgtctgcact gcttgaagta ttagacccgg aacagaatga acattttaca gatcattatc
      421 tggaagttgc attgaattta agtgatgtct tatttgttgc aacggccaat gacctttcta
      481 cgattccaag accactgctt gaccgtatgg agatcatcga ggtatcaagt tatacagaca
      541 atgagaagta tcacattgcg aatgactatc tggtaaagaa acagatggaa gcaaatggat
      601 taaatgatac acagattcac tggaaggaag atgccctgag atttctgatc acagattaca
      661 caagagaagc aggggtcaga aacttagaga gaagaattgg acaagtttcg agaaaggtga
      721 caagagacat ctaccagaag aaacacagaa aagtaacgat cacaaagaaa gtgatcaaag
      781 attatcttgg aagacctgtt tatgaatggg aaaaagacac tctaagagat catgtcggaa
      841 ttgttcatgg actggcttgg acagctgttg gtggggtgac attaaagatt gaagtaaatg
      901 ttatgccggg aaaaggagcg attcaggtga caggatccct tggaaatgtc atgaaagagt
      961 ctgcacaggc ggcgatcagt tatatcagaa gccagagtag aaaatataag atcaaacagg
     1021 atttctttga aaaacatgat atacatattc atattccaga aggagccgtg ccaaaagacg
     1081 gaccatctgc tggaattacg atgacaacag ccgttttgtc agcgatcaca ggtaataaag
     1141 tctgtggaaa tctggcaatg acaggggaag ttacgatccg cggagatgtt cttatgatcg
     1201 gtggattaaa agaaaagctt ctcgcagcaa aacggcttgg aattacaaaa gtacttgttc
     1261 caaaatcaaa tgaaaaagat gtcaaagagt ttgaagagga agttgtcgaa ggacttgaga
     1321 tcgtctatgt aaaaactatg acacaggtga tcaaagaagc atttgtacat tagatcgaag
     1381 gaaatagaaa ctggaggcag tgatgaatta cgaagaagca atgaatttta tacaaaatac
     1441 gaataaattt ggaagtgttt taggacttga taatatcaaa gaattattag aaagacttgg
     1501 aaatccacag gatcagctta aagtggttca tattgccgga accaatggaa aaggatcaac
     1561 acttgcattt cttgcaggaa tcttcagaga aagtggatat cgtgcgggga ggtatgtctc
     1621 cccggcatct ttttgctacg aagagagatt tcgcatcaat gaagagaaca tttcgaaaga
     1681 agatctgtgc ttttatatgg aaaaaataaa gaaaatttca gaagagatgg tgagagacgg
     1741 gatgtcacat ccgacaatgt ttgagattga aacggcactc agttttcttt attttctcga
     1801 taaaaaagtg gatgttgtgt tattggaaac aggaatgggt ggcagacttg atgcaacgaa
     1861 cgtagttaag aaaccgatcg cggtgatcat tgcatcgatc ggaatggacc atatgcagtt
     1921 tcttggagac acgatagaaa agatcgcagc agaaaaagca gggatcataa aagaaggatg
     1981 tccagttatt tcatataaca atcaagaatc cgtaaacgaa gtgatcaagg aaaaagcaaa
     2041 agagatgcac tgcaaggtta cttttgtaaa ttcggaaggg atccgtgtgt taagtgaatc
     2101 tttaaatggg gagagctttt cttatcgctc atctgatgga agatggtatg aaaagataga
     2161 aatcccgctt cttggaagac atcagatcaa taatgctgcc ttggcattag aagcattgaa
     2221 tacgataaga aattattatt gtatcagtga atttcagaca gaagatggat tacgcaaaac
     2281 aatctggaga ggcaggatcg agatcttaga aaaggaccca ctggtcattt gtgacggagc
     2341 acataatccg gatggagcga aaagtctgtt aaatttttta caaaataatt ttacaaatag
     2401 acgattaatc tatataatgg gagtgttatc agacaaagac tatgaacaga tgatacagat
     2461 ccttacgccg atggcagcaa aagtttatac ggtagcacca gataatgcga gggccttatc
     2521 aagcaaggat cttgcagaat gtgtaagaaa atatcatcat gatgtagaag aaagacagag
     2581 actgaaagag tgtcttgaag aggtaaaaca acaagcagat aaagaagacg tgatcataat
     2641 ctgtggtaca ttatcatttc agaatgaatt aaaatagagg atagaaacag tggaaagatt
     2701 ccaaaagatc gtatggcatg agaaatacaa ggagacatac gagagactac aacaattaga
     2761 aatagatcga aagttttgcg gtcatgatat agaacatttt ttgtcggtgg caagaatcag
     2821 ttatctgatg atccttgaaa aaagacttcc tgtgtccaaa gatatcattt atgcgactgc
     2881 attgcttcat gatcttggga gagctgatca gtatgaaaag ggaatttctc acgaggaagc
     2941 gggagcgatt cttgccgagg agatcttaac ggactgtgga tataccagag aagagagaaa
     3001 gtttatgaca gatacgatct taaggcatag agatatgaaa gaagaaagcg aaagttttgc
     3061 atcaatcttt tatcgtgcag ataaactttc aagagattgc atccactgca aagcaagaca
     3121 ggaatgttat tggccggaag aaaagaaaaa caacaagtat atatactagg agagatgcta
     3181 tgattattgg aaataagaca tttgatacga aacatcatgg atacatcatg ggaattttga
     3241 atgtgacacc agattcattt tcagatggag gaaaatatga tcatttagat gcggcactaa
     3301 agcatgctga tgagatgatc agagatggag cagcgatcat tgatgttgga ggagaatcta
     3361 caagacctgg atacacgttg atctctgatg aagaagagat cgcaagagtc acacctgtga
     3421 tcgaagcatt aaaaaaggaa tttgatgtgc cagtatccct agatacttat aaatcaggag
     3481 ttgcaaaagc gggaatcgag gctggagcag atctgatcaa tgacatctgg gggcttaagt
     3541 gggatggaac tatggctgca gttttagcag aaacaggagt tgcttcttgc ttgatgcata
     3601 accgtaagga tacagattat aaggattatt taaatgatgt ggtagcagat ttagatgaga
     3661 cgatgaagat ggcaaaggaa gctggaatca agaaagaaac gatcatgctg gatccaggaa
     3721 ttggatttgc caaggatctt gatcagaatt tagagcttat gaaccatctg gagttattaa
     3781 atcagtggga tgtaccagtt cttcttggaa catctagaaa atccatgatc ggtttaacgc
     3841 tggatcttcc atcagatcag agagtagaag gaactgtggc aacaacggtc agtgnnnnnn
     3901 nnnnatgtta aagaaaatat gcgtgcgatg aagatgatag aagcaatttg tgcaaaatag
     3961 ggagaacaga tgatggatca gattacaatc aaagatttgg aagtttatgc aaatcatggt
     4021 ttgtataaag aagagaaagc acttgggcaa aaatttctgg tatctgcaat tttatcgctt
     4081 gatacgaaac ttgcaggtgt cagtgatcag atggattatt ccgttgatta tggtaaagta
     4141 tgtcatagga tcaaagagat ccttacagaa aatgacttta acctgatcga atgtgtggct
     4201 gaaacagtgg cgaagaaatt attacttgag ttttcattga tcagaaaact agagattgaa
     4261 gtaaaaaaac catgggcgcc gatcgggttg ccactcgact atgtttcagt gaagattaaa
     4321 agaggatggc acagagcgta tcttggagta ggatcaaata tgggagatcg tatggaatac
     4381 attaatcagg caatcaatgc gatcgaggca caggatgata caagagttgt tcatgtttcc
     4441 agtctgatcg agaccaagcc atatggcggt gtggtacagg atgatttttt gaatggatgt
     4501 attgctattg acacattaaa agagccaga
//
LOCUS       DAOMUE010000167         4519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5386_length_4519_cov_2.088486, whole genome shotgun sequence.
ACCESSION   DAOMUE010000167 DAOMUE010000000
VERSION     DAOMUE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5386_length_4519_cov_2.088486"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1754)
                     /locus_tag="PLC71_07680"
     CDS             complement(72..1754)
                     /locus_tag="PLC71_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRM02557.1"
                     /translation="MSRKTRTKSKTRNNAKSRAKKATVSTVKSTDTEETVIPTAEPAI
                     IKEEVAQPEPKEEIKIETPVEENQVIEQPDLGPRRSVVFIGSECYPFVKTGGLGDVMS
                     ALPKSLAKLNVDVKVILPRYKCIPWEYQEKMEYKGSFYMDLCSDGRQYYVGIMEYQED
                     GVIYDFIDNEEFFSWGNPYTNLIDDIPKFCYFSKAALAALNYLDWVPDVVHCHDWQAA
                     LVPLYLRTCFADTNVGRASAVLTIHNLKFQGIYDRKMIQYWSGLPDYVFNKDCLTQNW
                     LDANMLKGGITYCNRLTTVSNTYAGEIQTEEYGEGLAEHLRYHQNKIRGIVNGIDINI
                     WNPSTDKLLKANYDAKTAIENKKKNKKALQESLGLEVDEHKMVIGLISRLTNQKGLDL
                     VNDVIPGVMDGNTQVVVLGTGDSWYEEAFRSYENQYKGSFCAYIAYDENVAHNIYSGC
                     DALLVPSRFEPCGLTQLIAMRYGAIPIVRETGGLRDTVWPYNMFDNTGNGFTFDRYES
                     GLLYDTINRAKTCYFENRASWDDMVIRDMNKDVSWETSARQYKDMYVELTPR"
     gene            complement(1900..2166)
                     /locus_tag="PLC71_07685"
     CDS             complement(1900..2166)
                     /locus_tag="PLC71_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02558.1"
                     /translation="MLTILNSESLWIGTDMIQFNAIRDILDQEKIKYKYKTYNHLGEW
                     AGSGTLRGNFGSVGNPTSQSIQYEIFVARKDLEKAQAVMWNLLR"
     gene            complement(2296..3084)
                     /locus_tag="PLC71_07690"
     CDS             complement(2296..3084)
                     /locus_tag="PLC71_07690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM02559.1"
                     /translation="MNEKANYGNWVPEKALYTLFGAVIVLGVIAVAVQVALSEMVIAI
                     IVGVLCILTLVMAIYMLICHEAFAFGKGNMMAGVHEHLIKHLDWDGEGKLLDIGCGAA
                     ALTVHCAKAFPKAQITAMDHWGVEWNYAKEQCEKNAKIEGVADHIAFQKGDAAKLDFP
                     DETFDAVVSNFVFHEVRTAKDKRDVVKEALRVLKKGGVFSFQDMFSQKALYGDMEEFV
                     RILKEEGISEVHYIGNLEKKLDFIPGFVTTPWMISGMGIIYGKK"
     assembly_gap    3245..3254
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3255..3552)
                     /locus_tag="PLC71_07695"
     CDS             complement(<3255..3552)
                     /locus_tag="PLC71_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02560.1"
                     /translation="MVPFLYLAIKSLYWSKGATLSKFMWCSEESIKPYFIKAGKNLRY
                     KNLYRQMMDSLEDKEFPKLSQEVQRTIFFEFGSVEEHYKYRDAVKKAYPYRKIDE"
     gene            complement(3863..4456)
                     /locus_tag="PLC71_07700"
     CDS             complement(3863..4456)
                     /locus_tag="PLC71_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRM02561.1"
                     /translation="MAKRIITISREFGSGGRFIGEEVAQQLGIAYYSENIIDQIAQQS
                     GLSPEYIEENAELSPKKGFFAYAFSGRDITGKSVDDMLYEAQRKVILEIAEKEPCVMI
                     GRNTDFILKDRDDVLNVFIHGDMPEKIKRICKLYNVTEEGAVKLIKDTDKRRRTNYNF
                     YTEQKWGMARNYTLSLNSSQLGYKRCEKMIIDCVDIC"
BASE COUNT         1223 a         1023 c          597 g         1666 t
ORIGIN      
        1 tttttcttat aaatatttct tcttatcttc tttctgactc atacatgcaa cactatcaac
       61 aaatcacaat tttatcttgg tgttaattct acatacatat ctttgtactg tcttgcactt
      121 gtctcccatg atacatcttt attcatatca cggatcacca tatcatccca gcttgctcta
      181 ttctcaaagt agcatgtctt tgctctattg atcgtatcat acagaagtcc gctttcatat
      241 cgatcaaatg taaagccgtt acctgtatta tcaaacatgt tatatggcca aacagtatct
      301 ctaagtcctc ctgtctctct gacaattgga atcgcaccat atctcatagc aatcaattgt
      361 gttagtccgc atggctcgaa tcttgatgga actaaaagtg catcacatcc agaatagata
      421 ttgtgggcaa cattttcatc gtaagcaata taggcacaaa aacttccttt atattgattc
      481 tcgtaagaac ggaaagcttc ttcataccag gaatcacctg taccaagaac aactacctgt
      541 gtatttccat ccataacgcc tggaattacg tcattgacca gatcaagtcc tttttggttc
      601 gtaagacgag aaatcaatcc gatcaccatc ttgtgttcgt cgacctctaa tcctaaagat
      661 tcctgtagtg ctttcttatt cttcttttta ttttcgattg ctgtctttgc atcataattt
      721 gcttttaaca acttgtctgt agatggattc cagatgttga tatcaatacc atttacaatt
      781 cctctgatct tattctggtg atatcttaag tgctctgcaa gtccttctcc atattcttct
      841 gtctgaatct ctccagcgta tgtgttactt accgttgtaa gtctattaca ataagtgatt
      901 ccacctttta acatattcgc atccagccag ttctgagtca ggcagtcttt gttgaataca
      961 taatccggaa gtcctgacca gtattggatc atttttctgt cgtagatacc ttggaatttt
     1021 aggttatgta tcgtaagtac tgcacttgca cgtcctacat ttgtatctgc aaaacatgtt
     1081 cttagataaa gtggtaccaa cgctgcctgc cagtcgtggc agtgtactac atcaggaacc
     1141 cagtccagat aattcagtgc cgccaatgct gccttggaaa aataacagaa ctttggaata
     1201 tcatcaatca gatttgtata cggattaccc caagagaaaa attcctcatt gtcgataaaa
     1261 tcataaatta caccatcttc ctgatactcc ataataccta catagtattg tcttccatca
     1321 gaacaaagat ccatatagaa agagcctttg tattccatct tttcttggta ttcccaagga
     1381 atgcatttat accttggaag gattactttc acatctacat ttaatttagc aagactcttt
     1441 ggaagtgcag acattacatc tccaagacct cctgttttca caaatggata gcattctgag
     1501 ccaataaaaa cgacacttct tcttggtcca agatcaggct gttcaataac ctgattttct
     1561 tctactggcg tttctatttt gatttcttct tttggttctg gttgtgcaac ttcctctttt
     1621 attattgcag gttctgctgt tggaataaca gtctcttctg tatctgtact tttcactgtt
     1681 gaaacagttg ctttttttgc cctgcttttt gcattatttc ttgtcttact ttttgttctt
     1741 gtttttctgc tcatatttgc ctccaatctg caacatacat catgcggtct gcaatgaatt
     1801 tttatgatat tcttattata ccataaaaag aatggcactg catatcactt tagtaatata
     1861 cagtatcttt cctttcttca ttatcatcat gtatctactt tatctaagca aattccacat
     1921 tacagcctgt gctttttcta agtcttttct cgccacaaaa atttcatact gtatggactg
     1981 tgatgtagga ttccccacac ttccaaagtt tccacgaagt gttcctgatc ctgcccattc
     2041 tcctaagtga ttgtatgttt tgtatttgta cttaatcttt tcctgatcca agatatccct
     2101 gatcgcatta aattgtatca tgtctgttcc aatccataag ctttctgagt ttaatattgt
     2161 aagcatactt ttctcctttc tcgcaatttc caattttatt aaactggatt tacccccctc
     2221 ttttgcagca taccgaagcc tctttcaacc tgttaattaa tttcttatat tttatcacaa
     2281 tttcctgact gtgtcctatt ttttcccata aataattccc atcccgctga tcatccatgg
     2341 tgttgttaca aatcccggaa taaagtctag cttcttttcc agatttccta tgtagtgaac
     2401 ttctgaaatt ccttcttctt ttaagattct tacaaactct tccatatctc cgtacaatgc
     2461 tttctgtgaa aacatatcct gaaaagaaaa tacaccgcct tttttcaaaa ctcttaatgc
     2521 ttcttttaca acatccctct tatcttttgc tgttcgcact tcatgaaaca cgaaattgct
     2581 tactactgca tcaaatgtct catcagggaa atccagtttt gctgcatctc ctttttgaaa
     2641 agcgatatgg tctgcaactc cttcaatctt tgcatttttc tcgcactgct cttttgcata
     2701 attccattcc acaccccagt gatccatcgc tgtgatctgt gcctttggaa atgctttggc
     2761 gcaatgaaca gtaagtgctg ctgctccgca tccgatgtct agaagtttcc cttcaccatc
     2821 ccaatcaaga tgtttaatca gatgttcatg tactcctgcc atcatatttc cttttccaaa
     2881 tgcgaatgct tcatgacaga tcagcatata aattgccatt accagagtca aaatgcataa
     2941 tacgcctact atgattgcaa tgaccatttc acttaaagct acttgtactg ctaccgcaat
     3001 aacacctaaa acaattactg ctccaaataa tgtatacaat gctttttcag gtacccagtt
     3061 tccataattt gctttttcat tcattcttct tttctccctt ttattagtgt tcgctaacta
     3121 ttctcatcaa ctttcctgta cagatgatag aatctaacat cttagcaaaa ccttttggat
     3181 caagaatctg catatgcata tgattctcat cttgaaatct gtgcaaattg acctccatca
     3241 aaaannnnnn nnnntctcat caattttcct gtacggatac gccttcttta cagcatcacg
     3301 atatttataa tgttcctcga cacttccaaa ctcaaagaaa atggttcttt gaacctcttg
     3361 tgacagcttt ggaaattctt tatcttccaa actgtccatc atctgtcgat atagattttt
     3421 ataccgcaga ttttttcctg cttttataaa atatggcttt atactttctt cactgcacca
     3481 cataaattta cttaaagtcg ctcctttact ccaataaagg ctcttaatcg ccagatacaa
     3541 aaatggaacc ataaccttcc ttgtcgatct tgaaatctgt gcaaattgac ctccatcaaa
     3601 aaagtatgct caattggaat tttcatctga gaaagaaatg tgagtcctaa atctgctcca
     3661 attgatgaag cgacaataag cgttattttt tatattcctt gcttttgcaa atactcttct
     3721 atctgatgaa tttcatcttg tttactttga taactttgtt cccatcattt gaaattccat
     3781 ttttatattc ctccttatag ttttagtata tcagattcaa aaaatatttc ctatatgaga
     3841 taataagaaa aattcctata atctaacaaa tatccacaca atctatgatc atcttctcac
     3901 accttttata tcctagctgt gaactgttta atgacaatgt ataattcctt gccattcccc
     3961 atttttgttc tgtatagaaa ttataatttg tccttcgtct tttatctgta tcctttataa
     4021 gttttactgc cccttcttct gtaacattat ataacttgca aatacgtttg atcttctctg
     4081 gcatgtctcc atgtataaac acatttaaca catcatctct atccttcagt ataaaatcag
     4141 tatttctccc aatcataacg catggttctt tttctgcaat ttccaaaatt acctttctct
     4201 gtgcctcata cagcatatcg tccacggatt tccctgtaat atcacgaccc gaaaatgcat
     4261 aggcaaaaaa tcctttcttt ggggataatt ctgcattttc ttcgatatat tctggtgata
     4321 atcctgactg ttgcgcaatc tgatctataa tattttcact ataataagca attccaagct
     4381 gttgtgctac ttcttctccg ataaatcgtc caccgcttcc aaattctcta ctgattgtaa
     4441 tgattctttt tgccataata ccttcctcct taatgtgtta atcgctctac ttttattttt
     4501 tggattttct ttaaaaaga
//
LOCUS       DAOMUE010000168         4501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5421_length_4501_cov_2.153846, whole genome shotgun sequence.
ACCESSION   DAOMUE010000168 DAOMUE010000000
VERSION     DAOMUE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5421_length_4501_cov_2.153846"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..702
                     /locus_tag="PLC71_07705"
     CDS             166..702
                     /locus_tag="PLC71_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRM02562.1"
                     /translation="MGFEFQILDKLQTIHTPLLDRFMVAITKLGDAGIVWIILTVILL
                     LIPKTRKSGVYMAVALIADLIICNVILKPIVARIRPYSINQTVQLLVTPLKDYSFPSG
                     HTAASFASVSALYFAGRKRMAAGALIVSVLIAFSRMYLYVHYPTDVLGGLIIGLLCGW
                     IADMIIQKVMEKRSRKSR"
     gene            840..1085
                     /locus_tag="PLC71_07710"
     CDS             840..1085
                     /locus_tag="PLC71_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6465 family protein"
                     /protein_id="HRM02563.1"
                     /translation="MIKKKESSMKTKIEVQFQERNIDVKDTEKLVKENLKAAGVKMNT
                     IVNLDIYYQPEEGDIYYVATTKDGKELSNEEALKIEE"
     assembly_gap    1295..1304
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1305..>1873
                     /locus_tag="PLC71_07715"
     CDS             <1305..>1873
                     /locus_tag="PLC71_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM02564.1"
                     /translation="LSIKWKDFYKNVFALVVPMALQNLINVGVTAADVVMLGKVSEKV
                     LSGASLAGQIQYIMVLFLFGLTSGATVLTAQYWGKGDKTAIEKILALGIRSAVIITAI
                     FTIAAFTIPASLMRIFTNDPQVIAEGVKYLRIVACSYIIMGITNVYLYVMRSIERVIV
                     ATVVYLVSFLCNVVFNAIFIFGLFGFPAL"
     assembly_gap    1874..1900
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1901..2687
                     /locus_tag="PLC71_07720"
     CDS             <1901..2687
                     /locus_tag="PLC71_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM02565.1"
                     /translation="IRGAAIATLIARIMELVLVCGYAKIYNKDVKFRVHYFLHQDKLL
                     VRDFLVYSLPVVLNEVMWGLGTSANTAVLGHMGSSAVAANSVAQVARQLATVVSFGLS
                     SATAIYLGKTIGERKFDHARAYAKRFIILSVVMGVIGGGLILIASPVVISFLSLSATA
                     RYYLKMMFYVMSYFVIAQAFNTTMVVGIFRSGGDTKFGLIMDVSTMWGCSILLGALAA
                     FVLHWSVPAVYVLLMSDELIKVPITLIRYRSFKWLKDVTRDNV"
     gene            2723..3727
                     /gene="selD"
                     /locus_tag="PLC71_07725"
     CDS             2723..3727
                     /gene="selD"
                     /locus_tag="PLC71_07725"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRM02566.1"
                     /translation="METEVKLTKLAQCAGCGAKVGAGTLAKLLDGIPTRSDERLLVGF
                     DKSDDASVYKIDENTALVQTLDFFPPIVDDPEMFGEIAAVNAISDIYAMGADPKLALN
                     IMCVTKDMPKESIQAILRGGYKKAYEAGVIITGGHTIHDQEPKYGLSVSGFVHPQKFL
                     TNSGARPGDILILTKPLGVGILTTSAKAEFIDQKVMDKIYDQMRQLNKYARDIMVKYE
                     VHSCTDVTGFGLLGHGYEMAQGSDVTIHFMTEEIPYHKEALSMADLGMIPEGASKTKK
                     INRALEDILYDPQTSGGLMIAVSENDAKNLLEDLQNTIPCAKIIGYIEEKEEKSIILE
                     "
     gene            3825..>4501
                     /locus_tag="PLC71_07730"
     CDS             3825..>4501
                     /locus_tag="PLC71_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM02567.1"
                     /translation="MKDQFQRTIDYARISITDRCNLYCTYCRPDHEEMLSHGEILRYE
                     EILRVCKILTTLGIDKFKITGGEPLVRKGCSDFIRELKKTDGVNKVTLTTNAILLSKD
                     LVKLAEAGVDGINISLDFMDQEKYKKITGFDGYKQVISVINKAINIGLNIKINVVLTE
                     QTTMEDIQKFIDYMKDHKICIRFIEQMPLGNKKTTIALTRNEIRKNLKDQGIRFHKTE
                     QRMGNGPA"
BASE COUNT         1563 a          634 c          985 g         1282 t
ORIGIN      
        1 agagaatatt ccgtgtatca gtattggaga aaagacaacg aagcagcttc gagaaatagg
       61 agtacggcat attttagaat ctaaggatac aacctatgta tcaatgtttt ataaaatcaa
      121 agaatcaatg ttataataga aaaagaaagg cagagtaaga ttattatggg atttgaattt
      181 cagattttag acaaacttca aacgatccat actccattat tagacagatt tatggtagct
      241 attacgaaac tgggagatgc agggatcgta tggatcattt taacagtcat tttattacta
      301 ataccaaaaa caagaaaatc aggagtatac atggcagttg cattaattgc agatttgatc
      361 atttgtaatg tgatcttaaa accgatcgtg gcaagaataa gaccatatag cattaatcag
      421 acggtccagc tgttggtcac accattgaag gattattctt ttccatcggg acatacagct
      481 gcatcatttg catcggtcag tgcgctgtat tttgcgggaa gaaagagaat ggcagcagga
      541 gctttgatag tatctgtttt aatcgcgttt tcaagaatgt atttatacgt tcattatcca
      601 acggacgttt taggaggttt gatcattggg ctcttatgtg gatggatcgc agatatgatc
      661 atccaaaaag ttatggaaaa aagatcaagg aaaagtagat agattttgta ctaaatcaga
      721 ttcaagttga ataaattgga aaaaaaggct ataatatgaa ttataaatcg tatgtagaat
      781 acaatgtacg catacaagaa tctaataata atcacatcaa gagttgacat aaagagtaga
      841 tgatcaagaa gaaggagagt agtatgaaaa caaaaatcga agtacagttt caggaacgca
      901 atatagatgt aaaagacaca gagaaacttg ttaaagaaaa tttaaaagca gctggtgtta
      961 aaatgaacac gatcgtaaat ttagatatct attatcagcc agaagaaggc gatatctact
     1021 atgttgcaac aacaaaagat ggcaaagagc tcagcaatga agaagcatta aaaatcgaag
     1081 aataaactac atatctataa atgccaaaaa attaaattct aattataaag gtaaagacca
     1141 tttaataaat atcgcaatga tgaatctgca gatgatttta ttaagtggtt tttatttttc
     1201 atttttgggg atactaaatg aaatacaggc aggcaatatg tgcttgacaa gcagacaaag
     1261 cttctatatc ctattatgag gtaatgaaaa gaacnnnnnn nnnnatttaa gcatcaaatg
     1321 gaaagatttt tataaaaatg tatttgcgct ggtcgttccg atggcattgc agaatctgat
     1381 caacgttgga gtaacagcag ccgatgttgt gatgcttgga aaagtcagtg aaaaagtatt
     1441 atcaggggcc tctcttgctg gccagattca gtacattatg gtactgtttt tgtttggatt
     1501 aacatcgggg gcaaccgttc ttactgcaca gtactgggga aaaggtgaca agacagcgat
     1561 tgaaaagatc ttggcattag ggattagatc agcagtgatc ataactgcga tattcactat
     1621 cgcagcattt acaattccag catcgttgat gagaattttt acaaatgatc cacaggtgat
     1681 cgcagaagga gttaagtatt taaggatcgt tgcatgttca tatattatca tgggaattac
     1741 caatgtatat ttatatgtca tgcgaagcat tgaacgagtg atcgtggcaa cagttgttta
     1801 tctagtatca tttttatgca atgtggtttt taatgcgatc tttatttttg gattatttgg
     1861 attcccagct cttnnnnnnn nnnnnnnnnn nnnnnnnnnn aatccgtgga gcagcgattg
     1921 cgacattgat cgcaaggatt atggaacttg ttctggtttg tggttatgca aaaatatata
     1981 ataaagatgt aaaattcaga gttcactatt tccttcatca ggacaaactg ttagtgagag
     2041 atttcttagt ctattccctc ccagttgttt taaatgaggt aatgtggggg cttggaacat
     2101 cagccaatac agcagttctt ggacatatgg gaagttctgc agttgcagcc aattctgttg
     2161 cacaggtggc gagacagtta gcaacggttg tgtcttttgg gctatccagc gccacagcga
     2221 tctatcttgg aaagacgatc ggtgagagaa aattcgatca tgcaagagcc tatgccaaac
     2281 ggtttatcat tttaagtgtg gtgatgggag tgatcggagg aggattgatc ttaattgcat
     2341 ctccagttgt aatttctttc ttatccttaa gtgcaactgc aagatactat ctgaaaatga
     2401 tgttttatgt gatgtcttat tttgtgatcg cgcaggcatt taatacaaca atggtcgttg
     2461 gaatcttccg atctggcggc gatacgaagt ttggcttgat catggatgta tcaacgatgt
     2521 ggggatgttc gattttatta ggagcattgg ctgcatttgt attacattgg agtgtgccgg
     2581 cagtttatgt attattgatg agtgatgaat tgatcaaagt accgatcaca ttgatcagat
     2641 atcgcagttt caagtggtta aaagatgtaa caagagataa tgtttaagag agatcatgaa
     2701 catttacggg aggataaggt ctatggaaac agaagtgaaa ttaacaaaac tcgcccagtg
     2761 tgcaggatgt ggggcaaaag ttggagctgg aactttagca aaattattag acggaattcc
     2821 aacaagatca gatgaacggt tattagttgg ttttgataaa agtgatgatg cgtctgtcta
     2881 taaaattgat gaaaatacag cacttgttca gacattggat ttcttcccgc cgatcgttga
     2941 cgatccagaa atgttcggag agattgcagc agtgaatgcg atcagtgata tttatgcgat
     3001 gggagcagat ccaaagcttg ccttaaatat catgtgtgtc acaaaagata tgccaaaaga
     3061 aagcattcag gcaattttac gaggtggtta taaaaaagca tatgaagcag gagtgatcat
     3121 cacaggtgga catacgattc acgatcaaga gcccaaatat ggtttatctg tttctggatt
     3181 tgtacatcct caaaaatttt tgacaaacag cggggcaaga cctggagata ttttaatttt
     3241 gaccaaaccg ttaggagttg gaattttaac aacatcagca aaggctgaat ttatcgatca
     3301 aaaagtaatg gataagatct atgatcagat gagacagctc aataaatatg ccagagatat
     3361 tatggtaaaa tatgaagtgc acagttgtac ggatgttact gggtttggat tacttggaca
     3421 tggttatgag atggcacagg gcagtgatgt aacgattcat tttatgacag aagagattcc
     3481 atatcacaaa gaagctcttt ctatggcaga ccttggaatg atcccggaag gtgcttcaaa
     3541 aacgaaaaaa ataaatagag cattggaaga tattttatat gatccacaga catctggagg
     3601 attgatgatc gcagttagcg aaaatgatgc aaaaaattta ttagaggatt tacaaaatac
     3661 aatcccatgt gctaaaataa ttgggtacat agaagaaaaa gaagaaaaat caatcatatt
     3721 agaatagaag aacgaacaga caggaagaat gttaaaacat ggtttttcct gtctgatttt
     3781 aggcaggcgt tattcttggt tgagaataac gataggagaa agagatgaaa gatcaatttc
     3841 aaagaaccat tgattatgca agaatatcga tcacggatcg ttgcaattta tattgtacct
     3901 attgccgccc tgatcatgag gaaatgttgt cacatgggga gattttaaga tatgaggaga
     3961 tcctaagagt atgtaagatt cttacaacgc ttggaattga caaatttaag atcacaggtg
     4021 gcgaaccgtt ggtacgaaaa ggttgttcag attttatccg tgaattaaag aaaacggatg
     4081 gagttaacaa agttacgtta acaaccaatg caatcttatt atcaaaagat ttagtgaaat
     4141 tagcagaggc aggagtggat gggatcaata taagtcttga ttttatggat caggagaaat
     4201 ataaaaagat cacaggattc gatggataca aacaggtcat atcagtaatt aataaagcga
     4261 tcaatatagg attgaatata aagataaatg ttgtgttgac agaacagacg acgatggagg
     4321 atattcaaaa atttatagac tatatgaaag atcataaaat ctgtatacga tttattgaac
     4381 aaatgccact tggaaataag aagacaacga tagcattaac aagaaatgaa atcagaaaga
     4441 acctaaaaga tcaagggatc agatttcaca aaaccgaaca aagaatggga aatgggccag
     4501 c
//
LOCUS       DAOMUE010000169         4422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5554_length_4422_cov_2.820472, whole genome shotgun sequence.
ACCESSION   DAOMUE010000169 DAOMUE010000000
VERSION     DAOMUE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5554_length_4422_cov_2.820472"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1223)
                     /locus_tag="PLC71_07735"
     CDS             complement(63..1223)
                     /locus_tag="PLC71_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM02568.1"
                     /translation="MKDIKVTDSVKYIGVDDHDIDLFESQYVVPNGVSYNSYVILDEK
                     VAVMDTVDACKTDEWLANLEEALDGRSVDYIVVSHMEPDHAASLQVLAEKYPEAKVVG
                     NAKTFNMIPQFFTFDLEGRKVVVKEGDTLSLGSHTLQFVMAPMVHWPEVMVAYETSEK
                     ILFSADGFGKFGALDADEDWACEARRYYFNICGKYGVQVQNLLKKAAGLDIEIICPLH
                     GPILKENLGYYIGLYDTWSKYEPENEGILIAYASIHGNTAAAAKKLAEILEAKGAPKV
                     VVADLSRDDMAEVIEDAFRYDRLVLAAATYDAGIFPCMEDFLHHLKAKNYQKRKVAFM
                     ENGSWAPMAAKIMKGIVEGFKNIEMVDPVVTIKSTMNDENIKTMEELADNLL"
     gene            complement(1420..2865)
                     /locus_tag="PLC71_07740"
     CDS             complement(1420..2865)
                     /locus_tag="PLC71_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRM02569.1"
                     /translation="MYDVTSIQNLKKDVLYFVAKASFEGTLEEERDLIPEKMIEGPEP
                     TFRCCIYKEREIVRQRIRLAEGKAPGAEDDGNIVQVIKSACADCPISSYVVTNNCQNC
                     LGKDCIKACRFGAIEPGHTRSRIDPQKCKECGMCAKACPYNAIAHVSRPCKDSCPVDA
                     ISYDEYGVSVIDEEKCIRCGQCAAKCPFGAIGTKTWITNVIADLKAGKKVYAILAPAT
                     EGQFGKDITMESWRQAVKKAGFEDLIEAGLGGDMTTCSEAEEWLEAYRNGEKKTTSCC
                     PGFVNMIRKHYPDLADMISTTVSPMCAVSRMIKAKDPDAVTVFVGPCVAKKSEVADQK
                     IEGNADYALNYNEILAIMKAKDVELEPAENTYQDSTIFGKFYGNSGGVTDSVLEYMKE
                     TEQNEDIKVCKANGAAECKKALMFLQRGRLPEDFIEGMACEGGCVGGPSRNEEIIKAR
                     KVRETMIKSADDRKITDNLKRYDMDSFSMHR"
     gene            3227..4333
                     /locus_tag="PLC71_07745"
     CDS             3227..4333
                     /locus_tag="PLC71_07745"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HRM02570.1"
                     /translation="MRPYRYDIVGSFLRPDYLKDARAEYAEGTLSADQLREVEDKAIK
                     ELVEKEKAVGLKAVTDGELRRRYWHLDFLASLVGVEEIKADHWSVAFKGHQPKAATLE
                     IVDKIDFDENSEFLDHFSYLKEIAGDVDCKMTIPSPAMLHLICCVRGSETYKAIDRYK
                     NEDDLYHEIALAYQKAIKAFYARGCRYLQFDDTSWGEFCDQNKRDDYASRGLDLDQIA
                     KNYVKMINEALEAKPEDMTITMHICRGNFRSTWFSSGGYEPVAETLFGGCKIDGFFLE
                     YDSDRSGDFKPLRFIKDQKVVLGLVTSKDPELEDKTEIKERIKEAAQYVALDQLCLSP
                     QCGFSSTEEGNILTDEDQWKKMTLIKEIAEEVWG"
BASE COUNT         1209 a          888 c          753 g         1572 t
ORIGIN      
        1 tgcttattta ttttttatca tatactgaaa gactacagtt ggccaactta caaatttaaa
       61 atttacagta aattatcagc taattcttcc atagtcttaa tattctcatc attcatcgta
      121 gatttgatcg ttacaactgg atctaccatc tcgatattct taaatccttc tacgatacct
      181 ttcatgattt ttgctgccat tggtgcccat gatccatttt ccataaatgc aactttacgt
      241 ttctgatagt tcttagcttt taagtgatgt aaaaagtctt ccatgcatgg gaagattcct
      301 gcatcgtatg ttgctgctgc cagtaccagt ctatcatagc ggaatgcatc ttcgatcact
      361 tctgccatat catctctgga aaggtctgct actacaactt ttggagctcc ttttgcttct
      421 aagatttctg ctaatttctt tgctgcagct gctgtatttc catggataga tgcgtatgcg
      481 atcaggattc cttcgttctc tggctcatat ttgctccatg tgtcgtataa tccaatataa
      541 tatccaaggt tttcttttaa aattggtccg tgtaatggac agatgatctc aatatctaat
      601 cctgcggctt tctttaagag attctgtacc tgtactccgt atttaccaca gatattgaaa
      661 taataacgtc ttgcttcaca tgcccaatct tcatctgcat ctaaagctcc gaatttacca
      721 aatccatctg ctgagaataa gattttctct gatgtttcat atgctaccat aacttctggc
      781 cagtgaacca ttggtgccat aacgaactgt aatgtatgag atccaagaga taatgtatct
      841 ccttctttta ctacgacttt acgtccttct agatcaaatg taaagaactg agggatcata
      901 ttaaatgtct tggcattacc tacaacctta gcttctggat atttttctgc taatacctgt
      961 aagcttgcag catggtctgg ttccatatga gatactacaa tgtaatctac gcttcttcca
     1021 tctaatgctt cttctaagtt tgcaagccat tcatctgtct tgcatgcatc cacagtatcc
     1081 ataacggcta ctttctcatc taagatcaca taagagttat aagaaacacc atttggtact
     1141 acatactggc tctcaaataa gtcaatgtca tgatcgtcta caccaatata ttttactgaa
     1201 tctgtaactt taatatcctt cattgtctta cctccaaaat taaattcatt ttgctttttc
     1261 aacttttctt actataacat agttttgcca aaatatctac tagtaatagt tactatttct
     1321 aatacaattt cttttcagat acaaagatcc ccaaaacaaa acttcaatct tgctccaggg
     1381 attctttatc atctctttgg atgcttcatg caactctgat tatctatgca ttgagaagct
     1441 atccatatca taacgtttta agttgtctgt gatcttgcga tcatctgcgg atttgatcat
     1501 tgtttctctg actttacgag ctttgatgat ctcttcgtta cggcttggtc caccaacgca
     1561 tccgccttcg catgccatac cttcgatgaa gtcttctggt aatcttcctc tctgtaagaa
     1621 cattaaagct ttcttacatt ctgctgcacc gttagctttg catactttga tatcttcgtt
     1681 ctgttctgtc tctttcatgt attctaatac agaatctgtt actccaccag agtttccata
     1741 gaatttaccg aagatcgtag aatcctgata tgtattctct gctggttcta attctacgtc
     1801 ttttgctttc ataattgcta agatctcgtt gtagtttaat gcataatctg catttccttc
     1861 gatcttctgg tctgccactt ctgatttctt ggctacacat ggtccaacaa atactgtaac
     1921 tgcatctgga tcttttgctt tgatcattct tgatactgca cacattggag atactgttgt
     1981 agaaatcata tctgcaagat ctggataatg tttgcgaatc atgtttacga atcctggaca
     2041 gcatgaagtt gttttcttct caccgtttct gtaagcttct aaccattctt ctgcttcgct
     2101 gcatgttgtc atatctccac ctaaacctgc ttcgatcaga tcttcaaatc cagctttctt
     2161 tactgcctgt ctccagcttt ccattgtaat atctttacca aactgtcctt ctgttgctgg
     2221 ggcaaggatc gcgtagactt tcttacctgc tttcaagtct gcgataacgt ttgtgatcca
     2281 tgtctttgtt ccgatcgcac cgaatggaca ttttgctgca cactgtccac aacggataca
     2341 tttttcttca tcgatcactg atactccata ctcatcataa gaaattgcat ctactggaca
     2401 gctgtcttta catggtcttg atacatgagc gatcgcattg tatggacagg cttttgcgca
     2461 cataccacat tccttacatt tctgagggtc gattcttgat cttgtatgtc ctggttcgat
     2521 cgctccgaaa cgacatgctt tgatacagtc ttttccaaga caattctgac agttgtttgt
     2581 tacaacatag cttgagatcg gacagtcagc acatgctgat ttaattacct gcacgatatt
     2641 tccatcatct tctgctcctg gagctttacc ttctgctaaa cgaattctct gtcttacgat
     2701 ttctctttct ttgtagatgc agcaacggaa tgttggttct ggtccttcaa tcatcttctc
     2761 tgggatcaga tctctttctt cttctaaagt cccttcaaac gatgcctttg ctacgaaata
     2821 cagtacatct tttttcagat tctgaatgct tgttacatca tacataatag tttacccttc
     2881 cttaaaaaac acttaactaa aacaccctag atattgttat ttttttatct atgtgcatta
     2941 taacaaatat tttctttttt ctctacaatg cttaaagaaa agtacaaaaa atatgcaatt
     3001 tttttaaaat tgtacaatta acattttctt aacgatgact ttgtccgtat attgcataca
     3061 attattgtta aaagaaagac ataattgtct gaaaattttt atcattataa cttgaagtta
     3121 tggcaaacta ttatttttat gtatttcaca agccttattc tatctgttat tgtatagctt
     3181 atcgatagaa aattattaga cttatataaa tggaaaggat agatttatga gaccatacag
     3241 atatgatatt gttggtagtt ttttaagacc agattattta aaagatgcac gtgctgaata
     3301 tgcagaagga actttatctg cagaccagtt gcgtgaagtt gaagataaag cgatcaaaga
     3361 gttagttgaa aaagagaaag ctgttggatt aaaagcagtt actgacggag agttaagacg
     3421 tcgctattgg catcttgatt tccttgcttc tttagtcgga gtcgaggaga tcaaagctga
     3481 tcactggtct gttgctttta agggacatca gccaaaagcc gcaactcttg agattgttga
     3541 caaaattgat tttgatgaaa acagtgaatt cttagatcat ttctcttact tgaaagaaat
     3601 tgctggggat gttgactgca agatgacaat tccttctcct gcaatgcttc atctgatctg
     3661 ttgtgttcgt ggaagcgaaa cttataaagc gatcgatcgt tataaaaatg aagatgatct
     3721 gtatcatgag attgctcttg cttatcagaa agcgatcaaa gcattctacg ctcgtggatg
     3781 ccgttactta cagtttgatg atacatcttg gggtgaattc tgtgatcaga ataaacgcga
     3841 cgattacgca agccgtggtc ttgaccttga ccagattgct aagaattatg tgaaaatgat
     3901 caacgaagct ttagaagcta aacctgaaga tatgacgatc acaatgcata tctgccgtgg
     3961 taacttccgt tctacatggt tctcttctgg tggttatgag ccagttgctg agactttatt
     4021 cggtggatgt aagatcgatg gtttcttctt agagtatgat agtgaccgtt ccggtgattt
     4081 caaaccatta cgttttatca aagatcagaa ggttgtgtta ggtcttgtta cttctaaaga
     4141 tccagaactt gaagataaaa ctgagattaa agaacgtatt aaagaggctg ctcagtatgt
     4201 tgcacttgac cagctttgct taagtcctca gtgtggtttc tcttctacag aagaaggaaa
     4261 tatcttaact gatgaagatc agtggaagaa gatgactctt attaaggaga ttgctgagga
     4321 agtttgggga taggatatcc gtgatacata tttcataata atatagtaaa taaaagaatc
     4381 cggcattaca tttttgtgtt tgccggattt ctttatatcc aa
//
LOCUS       DAOMUE010000170         4399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5596_length_4399_cov_1.959254, whole genome shotgun sequence.
ACCESSION   DAOMUE010000170 DAOMUE010000000
VERSION     DAOMUE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5596_length_4399_cov_1.959254"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLC71_07750"
     CDS             <1..301
                     /locus_tag="PLC71_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRM02571.1"
                     /translation="AIIVNTPKSADEIKPMLNGYQGKVYTVDARKISIQALGKYFPNS
                     PMLAAAVAVSGVMKKDAFISEMRASYQHKFAKKPEVIDGNMQALELTYEELKDVL"
     gene            331..633
                     /locus_tag="PLC71_07755"
     CDS             331..633
                     /locus_tag="PLC71_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM02572.1"
                     /translation="MRTDISKINEQTPHQDLTEGLMLFAGGTSKQFHTGEWRTRTPIL
                     DQDLCRQCMVCSMVCPDSSIPLKDGERVEFDYDHCKGCGICAKACPFDAITMKEGR"
     gene            635..1813
                     /gene="porA"
                     /locus_tag="PLC71_07760"
     CDS             635..1813
                     /gene="porA"
                     /locus_tag="PLC71_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HRM02573.1"
                     /translation="MARKDRMSGNEAVSFAIRQINPDVMPAFPITPSTEIPQMVSTYI
                     ANGEIDTEFVPVESEHSAMSAAIGSEAAGARTLTATSSAGLALMWEELLLAASNRMPI
                     ALALVNRTLSGPININCDHSDGMGARDTGWIQIYAENNQEAYDNFIQAYPIAESAGVH
                     LPIMICQDGFITSHAVENIELLEDEEVKNFVGEYEPEEFLLNPGKPIAVGPYSVTSYA
                     MEAKKNQEIALENAKDAVLKVAKEFKKISGRSYGLFEEYKTKDADYIMLLIGSAAGTA
                     KQAVDDLRAKGKKVGVIKLRLFRPFPADELATALKGAKAVAIMDRTESYNGNGGPLGS
                     EVTAGLFRNKVMIDTVNYIYGLAGRDFTVQEVYDIFADLEDHIENGTKLEQFKYIGLR
                     "
     gene            1851..2768
                     /locus_tag="PLC71_07765"
     CDS             1851..2768
                     /locus_tag="PLC71_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRM02574.1"
                     /translation="MMSKKERLAPGHRMCAGCGATIGVRAVLRALHEEDHAVIANATG
                     CLEVSSFMYPYSAWEDSYIHNAFENAGATLSGVETAYKALKKRGKLPKDENFKFIAFG
                     GDGGTYDIGFQSLSGAMERGHDMVYVCYDNGAYMNTGIQRSSATPMFADTTTTPTGKE
                     SVGKMQNRKDLASIIADHDVAYVGQSTFTLNFNDLHKKAEKAIYTEGASFLNIMTPCP
                     RGWRYNTEDIMEICRLAVETCYWPMFEVDHGKWNLTYEPKKKLPVEDFLRAQGRFKHL
                     FKPGNEHLIEEFQKEVDRRWENLLYKCDR"
     gene            2796..3683
                     /locus_tag="PLC71_07770"
     CDS             2796..3683
                     /locus_tag="PLC71_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM02575.1"
                     /translation="MTLLTYQVFKTIADVGSFHKAADILGLTPSAISHAISSMENELG
                     FSVLTRSKAGVSLTNYGEQLLPYVNAVLNCDESLKQEIAELNGLKQGKVKIGVFSSVC
                     TSWLPEILHSFKKKYTEIDIEVFQGTYDDVVYWIKNGVVDLGFLSVSSAKDIPIEPLY
                     RDELLCVLPSGTKANKSTNYIDIEEMKHHQFVTQRDSTDADIQNFLKENSLQVQSNYH
                     VVDDLSTIALVEKGFGICLMPKLVMTDIPYKVDSYPTKPPASRIIGLAAMNPNFMAPA
                     VRTMFNHIVDKYQENEFKK"
     gene            3737..>4399
                     /locus_tag="PLC71_07775"
     CDS             3737..>4399
                     /locus_tag="PLC71_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRM02576.1"
                     /translation="MQVKGVRLYAKDDIRLEEFELPEIKDDEILVKVMSDSICMSTWK
                     MVKQGADHKRVPADVADHPIIIGHEFAGDIVKVGKKWQDKFKPGQKFAQQPAIPGQAE
                     SPGYSYTECGGAATYCIFPNDIIEAGCLWTHEGDSYFESSVAEPMCCVICGYKTNYHV
                     KDRYEHVMGTKAGGSIAILGGCGPMGLGAVSYALAIENKPAKVIVTDIDDAKLARANE
                     VIS"
BASE COUNT         1520 a          739 c          964 g         1176 t
ORIGIN      
        1 agcaatcatt gtaaatacac caaaatcagc cgatgagatc aagccaatgt taaatggcta
       61 tcaaggaaaa gtttatactg tcgatgcaag aaaaatctct atacaggctt taggaaaata
      121 ttttccaaac tctccaatgc tcgcagcagc tgttgcagtc agtggtgtta tgaaaaaaga
      181 tgcttttatt tctgaaatgc gagcttctta tcagcataaa tttgcaaaga aaccagaagt
      241 catcgatgga aatatgcagg cacttgaatt aacttatgaa gaattaaaag atgtattata
      301 aaaacgtatt ttaaatggag atataacaat atgagaacag atataagcaa aatcaatgag
      361 cagacacctc atcaggatct gacagaagga ctgatgttat ttgctggcgg aacttcaaaa
      421 cagtttcata caggggaatg gagaacaaga acaccaattt tagaccagga tctttgcaga
      481 cagtgtatgg tctgttccat ggtctgtcca gactctagta ttcctctaaa agatggtgaa
      541 cgagtagaat ttgattatga ccactgtaaa ggatgcggta tttgtgcaaa agcctgtcca
      601 tttgatgcaa tcacaatgaa ggagggaaga taagatggct agaaaagatc gtatgtcagg
      661 aaatgaggcg gtatccttcg caatccgcca gatcaatccg gatgttatgc cggcattccc
      721 aatcactcca tccacagaga ttccacagat ggtttctaca tatattgcaa atggagaaat
      781 cgacacagaa tttgtccctg tggaaagtga acattctgca atgagtgctg caatcggatc
      841 agaagctgcc ggcgcaagaa ctttaacagc aacatcttct gcaggacttg cattgatgtg
      901 ggaagaatta ttgcttgcag catcaaaccg tatgcctatc gcattagctc tcgtaaaccg
      961 cacattatcc ggaccgatca acatcaactg tgaccattct gatggaatgg gcgcaagaga
     1021 tactggatgg attcagattt atgcggaaaa caatcaggaa gcctatgata actttattca
     1081 ggcatatcca atcgccgaaa gtgctggagt ccatttaccg atcatgatct gtcaggatgg
     1141 atttatcaca agtcatgcgg ttgaaaatat tgaattacta gaagatgaag aagttaaaaa
     1201 ttttgttggt gaatatgaac cggaagaatt cttattaaat cctggaaaac caatcgcagt
     1261 tggaccatat tctgtgacaa gctatgcaat ggaagcaaag aaaaatcaag aaatcgcatt
     1321 agaaaatgcg aaagatgcag tcttgaaggt tgcaaaagaa tttaaaaaga tttctggaag
     1381 aagctatgga ttatttgaag aatacaaaac aaaagatgcc gattacatca tgttattgat
     1441 cggatcagct gctggaacag caaaacaggc agtggatgac ttaagagcaa aaggtaaaaa
     1501 agtcggtgtt atcaaactgc gtctgttccg tccattccca gccgatgaat tagccacagc
     1561 tttaaaaggt gccaaagcgg tagcaatcat ggaccgtaca gaaagttata acggaaacgg
     1621 tggacctctt ggaagtgaag taactgccgg attatttaga aataaagtga tgatcgatac
     1681 cgtcaactat atttacggac ttgccggaag agactttacc gtacaggaag tatatgatat
     1741 ctttgctgat cttgaagatc atattgaaaa tggaacaaaa cttgaacagt ttaaatacat
     1801 cggattgcgt taataggagg aatactgtga gtagttataa tgtaaaagaa atgatgagta
     1861 aaaaagagcg tttagctcca ggacatcgta tgtgtgccgg atgtggtgct acaattggag
     1921 ttcgtgcggt acttcgtgct ttacatgaag aagatcatgc tgtgatcgca aatgcgacag
     1981 gatgtcttga ggtatcttct ttcatgtatc catattctgc atgggaagac agctatatcc
     2041 acaatgcatt tgaaaatgca ggagcaacat taagtggagt tgagacagca tataaagcat
     2101 taaaaaaacg tggaaaactt ccaaaagacg aaaactttaa attcatcgca tttggtggag
     2161 atggtggaac ttatgatatc ggattccagt ctttatcagg agctatggaa cgtggtcatg
     2221 acatggttta tgtgtgttac gataacggag cttatatgaa tacaggaatc cagagatctt
     2281 ctgcaacacc aatgtttgca gatacaacaa caacaccgac aggaaaagaa tctgttggta
     2341 aaatgcagaa ccgtaaagac cttgcaagca tcatcgcaga tcatgatgta gcatatgtag
     2401 gccagagtac atttacacta aactttaatg atcttcataa aaaggctgaa aaagcgattt
     2461 acacagaagg ggcaagtttc ttaaatatca tgacaccatg tccaagagga tggagataca
     2521 atactgaaga tattatggaa atctgtcgtc ttgcagtaga aacatgctac tggccaatgt
     2581 ttgaagtaga tcatggaaaa tggaatttaa cttacgagcc aaagaaaaaa cttcctgttg
     2641 aagatttctt aagagcacag ggaagattta aacatttatt taaacctgga aatgaacatt
     2701 taattgaaga attccagaaa gaggttgaca gacgttggga aaatctgtta tataaatgtg
     2761 atagataaaa taataaagta aggaagtttt atggaatgac attattgaca taccaagttt
     2821 ttaagacaat cgcagatgta gggagtttcc ataaagcagc ggatatctta ggattaacac
     2881 cctctgcgat cagtcatgcc atctcatcga tggaaaatga attaggattt tcagtattga
     2941 caagaagcaa agcaggagtc agtctcacaa actatggaga gcagctatta ccatatgtaa
     3001 atgctgtatt gaactgtgat gaaagcttaa agcaggaaat tgctgaatta aatggattaa
     3061 aacaaggaaa agtaaagatt ggagtattct ccagtgtatg taccagctgg ctaccagaga
     3121 tcttacattc ttttaagaag aagtatacag agattgacat tgaagtcttt cagggaactt
     3181 acgatgatgt tgtatactgg atcaagaacg gagttgtaga tctcggattt ttatccgtat
     3241 ccagtgcaaa ggatattccg attgaaccat tatatcgtga cgaattactc tgtgttctgc
     3301 cgagtggtac aaaagcaaat aaaagtacaa attatattga tattgaagaa atgaaacatc
     3361 atcaatttgt aacacaacga gacagtaccg atgcagatat ccagaatttc ttaaaagaga
     3421 attcgctgca agtacaatcg aactatcatg tagttgatga tttatcaacg atcgcacttg
     3481 ttgagaaagg atttggaatc tgtctgatgc cgaaacttgt tatgacagat attccatata
     3541 aagttgatag ttatccaaca aaaccacctg caagcaggat catcggactt gcagctatga
     3601 atccgaattt tatggcgcct gctgttcgca caatgtttaa tcatattgta gataaatatc
     3661 aagaaaacga gtttaaaaaa taatttcctt gtgctaaaat atacctgtat ggattttata
     3721 ttttaggagg tagcatatgc aagttaaagg tgtaagatta tatgcaaaag acgatatccg
     3781 tctggaagaa tttgaactcc cagagatcaa ggacgatgag atccttgtta aggtgatgag
     3841 tgattccatc tgtatgtcta catggaaaat ggtaaaacaa ggagcagatc ataagcgagt
     3901 tcctgcggat gttgctgatc acccgatcat cattggacac gaatttgccg gagatattgt
     3961 gaaagtcggt aaaaaatggc aggataaatt caaaccagga cagaagttcg cacagcagcc
     4021 agcaattcct ggacaggctg aatctccagg atactcttat acagagtgcg gtggagcagc
     4081 aacttactgt attttcccaa atgatattat cgaagctgga tgcctttgga ctcatgaagg
     4141 agattcttac tttgaatctt ctgttgcaga accaatgtgc tgtgtcattt gtggttataa
     4201 aacaaactat cacgttaaag accgttatga acatgtaatg ggaacaaaag caggtggaag
     4261 tatcgcaatt cttggtggat gcggaccaat gggacttggt gctgtaagct acgcccttgc
     4321 catcgagaat aaaccagcca aagtgatcgt tacagacatt gatgacgcca aattagcaag
     4381 agcgaatgag gtgatctct
//
LOCUS       DAOMUE010000171         4379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5645_length_4379_cov_2.898242, whole genome shotgun sequence.
ACCESSION   DAOMUE010000171 DAOMUE010000000
VERSION     DAOMUE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5645_length_4379_cov_2.898242"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1726)
                     /locus_tag="PLC71_07780"
     CDS             complement(<1..1726)
                     /locus_tag="PLC71_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5717 family protein"
                     /protein_id="HRM02577.1"
                     /translation="MSESLENFTNIVKEDIKEAKTLFFSEAFKEEYLKDRREEKELYH
                     QLLLGRNRNQILEEFLLSSGQKKPVLLSAAKTIYQATIMNLDEPLEIKVKREGWGYLV
                     GRIKSDGTFLSLHKNVFQAEDFEDGTLTIYADMKTIPKDGDIDHLIIQTVYQTLQIEV
                     VYKEQKSERKKEEEFRQKKLIELYVDFCTGRITTSQLYERGNMVLDKMPDDPVKNRIY
                     DLMKLHLSILEGKGDLEEKIPEDASKEPLLIAQGYVWYLQAFYDKEEETIIRSRDEIK
                     ELYDQCEDGKIKGYLFWLYMNLSEELMKDAKLRMELIKELYQEGCQNPLLQFEGCCIL
                     GEDEQLLDEIDSYELWVLEFGAEEKILNSKLIGRICFLISRNKVFSEEVLWLFLCIYK
                     EYRQSDVLQGICALMIQGNCMDEDCHPYYEEALQRGVQLIGLQEAFLRTIPKGQYPLL
                     PEEILRYFTYSKSLGKEDQSRLFANVVQNRRKYHKVFANYEELIRAFLREELRRGQIN
                     EDLILLYRYYFEELLEDERSRQDLGNIIFVRQLICDNEFIQKAEISQRWKKNLDVCYL
                     REEKIYVEI"
     gene            1882..2967
                     /locus_tag="PLC71_07785"
     CDS             1882..2967
                     /locus_tag="PLC71_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent class II
                     aminotransferase"
                     /protein_id="HRM02578.1"
                     /translation="MSEKKQEFHGSDLEKVEAYYGIKKEDIIPFAGNVNPLGISPLLK
                     KSMASHIESISEYPDRDYKELRSTLALYCNVPMEHIIVGNGATEMISLTMQLLRPKHA
                     LLLSPTYSEYTREIDLVGGHVEEYFLREDLDFKLDLNDLISKLTDDIDLLAICNPNNP
                     TSSALNTEEITKILTHCKLHDIFVMIDETYVEFAPDIDTISAVSLTTKFDNFMILRGT
                     SKFFCAPGLRLGYGICGNLAFLERMNSIKNPWTINTLAALAGEAMFMDTDYIQTTKDY
                     IQSERTRCIDILSKRDNLKIYPAYANFILVKLLDGTTSFEMFERCIKSGLMIRDCASF
                     HGLDGEFIRFCIQKKEDNDRLLNLLTL"
     gene            complement(3051..3428)
                     /locus_tag="PLC71_07790"
     CDS             complement(3051..3428)
                     /locus_tag="PLC71_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRM02579.1"
                     /translation="MDTKIINAENAPAAVGPYCHAVEAGDFVFTSGQIGLDPQTQELK
                     EGVAAQTKQVLVNLGAVLKASDLTLDNVIKTTVFLADINDFAEINEIYEEAFGDNKPA
                     RSCVQAGALPKGALIEIEVIAVK"
     gene            complement(3458..4009)
                     /gene="lepB"
                     /locus_tag="PLC71_07795"
     CDS             complement(3458..4009)
                     /gene="lepB"
                     /locus_tag="PLC71_07795"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRM02580.1"
                     /translation="MSKENADKEQKRSFKREVFSWIACIVVTILLTEFILNFVIINAN
                     IPSGSMENTIMTNDKLIALRTSYWFNDPKRGDIIIFKYPDDETEWFIKRVIALPGETV
                     LVKDGKVYINGSKKALSEPYIKEEPVEDFGPYKVPKNGYFVMGDNRNNSNDAREWETH
                     YVSRDEVLGKAWFRYYPSIKVIK"
     gene            complement(4029..>4379)
                     /locus_tag="PLC71_07800"
     CDS             complement(4029..>4379)
                     /locus_tag="PLC71_07800"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HRM02581.1"
                     /translation="VVATKYGCRMQGTTDVAFTVLDVLGYLDEIPVCVGYEIDGEVTT
                     DFPTTGKLEKAKPVLEVLPGWKSDIRGIKNYEDLPENCRKYIEFVEEKIGYPITLISN
                     GPERHDIIRRGFED"
BASE COUNT         1248 a          965 c          658 g         1508 t
ORIGIN      
        1 aaatctctac atagatcttt tcttctctta agtaacaaac atccagattc ttcttccatc
       61 tctgactgat ctccgctttc tggataaatt cattgtcaca gatcaactgt ctgacaaaaa
      121 taatattacc taaatcctgt cttgatctct catcttccag caattcttca aaataatacc
      181 ggtataataa gatcagatct tcattgatct gacctcttct taattcttct cttaaaaaag
      241 ctctgatcaa ttcttcataa tttgcaaaca ccttatggta ttttcttctg ttctgtacaa
      301 catttgcaaa caggcggctt tgatcttctt ttccaagact cttactatac gtaaaatatc
      361 ttaagatctc ttctggaagc aatggatatt gtcccttcgg gatcgttctt aaaaaagctt
      421 cctgcaagcc aatgagctgt actcctcttt gaagtgcttc ctcataataa ggatgacagt
      481 cctcgtccat acaattgccc tggatcatca gtgcacagat tccctgcagc acatcactct
      541 gacggtattc tttataaata caaaggaaca accaaaggac ttcttcggaa aacaccttgt
      601 ttcttgaaat caaaaaacaa atccttccaa tgagtttgga attcaggatc ttctcctctg
      661 caccaaattc aagcacccat aattcataag aatctatctc atccaacagc tgctcatctt
      721 cgcctaaaat acagcatcct tcaaattgca aaagtggatt ttgacatcct tcctgatata
      781 attctttgat cagttccatt cgaagtttag catccttcat caattcttcg cttagattca
      841 tataaagcca gaacagatat cctttgattt ttccatcttc gcattgatca tagagttctt
      901 taatctcatc tcttgatctt atgatcgttt cttcttcctt atcataaaat gcctgaagat
      961 accaaacata gccctgcgcg atcaaaagcg gctctttcga tgcatcttct ggaatctttt
     1021 cttctaagtc tccttttcct tcaagaatag aaagatgcag tttcatcaga tcataaatac
     1081 gattcttaac cggatcatcc ggcatcttat caagaaccat atttcctctc tcataaagct
     1141 gacttgttgt aatccttcct gtacagaaat ccacatataa ttcgatcaat ttcttttgac
     1201 ggaattcttc ttcttttttt ctttcacttt tctgttcttt atagacaacc tcgatctgca
     1261 gagtctgata aacagtctga atgatcagat ggtcgatatc tccatccttc ggaattgttt
     1321 tcatatctgc atagatcgtc agagtgccat cttcaaaatc ctctgcctga aatacattct
     1381 tgtgaagaga caggaaagtt ccgtcacttt ttattctccc taccagatac ccccatcctt
     1441 ctctcttgac ttttatctcc aatggttcat caagattcat gatcgttgcc tgatagatcg
     1501 tctttgctgc actcaagaga accggctttt tctgtcctga actcagcaaa aattcctcca
     1561 agatctgatt cctgtttctc cccagcaaaa gctgatgata aagctctttt tcttctcttc
     1621 gatcttttag atattcttct ttaaacgctt cagaaaaaaa gagtgttttt gcctctttta
     1681 tatcttcttt cacgatattt gtaaaatttt ccaatgattc tgacataatc ttattctgct
     1741 ccttgccaac caaaagattt tgtgggtata attatgatgt aaatatcaaa aatacaaatt
     1801 caagtacacc catgcgtact tatcgcataa tatgtattat accaaatttc gacacaatag
     1861 aaaaggaaaa gaggaattac catgtcagaa aaaaaacaag aatttcacgg aagtgatctt
     1921 gagaaagtag aagcttatta tggaatcaaa aaagaagata tcattccctt tgccggaaat
     1981 gtcaatccgc tcggaatttc tcctttgtta aagaaaagta tggcttctca tattgaatcg
     2041 atcagcgaat atcctgaccg tgattataaa gagcttcgtt ccacccttgc actttactgc
     2101 aatgtcccaa tggaacatat cattgttgga aatggtgcaa ctgagatgat cagtctgacc
     2161 atgcagctgt tacgtccaaa acatgccttg ctgctttctc caacttattc tgagtataca
     2221 agagagatcg atctggttgg cggacatgta gaagaatatt ttttaagaga agatcttgat
     2281 tttaaactag atcttaatga cctgatttca aagcttaccg atgatattga tcttcttgcg
     2341 atctgtaacc caaacaaccc gacgtccagc gctttgaata ccgaagagat cacaaagatc
     2401 ctaactcatt gtaaattaca tgatatcttc gttatgatcg atgaaactta tgtagaattt
     2461 gcacctgata ttgatacgat ctctgcagta tccctaacaa caaaattcga taatttcatg
     2521 atacttcgtg gaacttccaa attcttctgc gctccaggac tgcgacttgg atatggaatc
     2581 tgtggaaatc ttgctttcct tgaacgtatg aacagtatca aaaatccttg gacgatcaat
     2641 acacttgctg ctcttgcagg agaagctatg tttatggata cagattatat tcaaacaaca
     2701 aaggattata tacaatctga acgtacacgc tgcattgata ttttatccaa acgcgataac
     2761 ctaaagattt atccagctta tgcaaacttt atccttgtaa aattattaga tggcacaact
     2821 tcttttgaaa tgtttgaacg ttgcattaag tctggcctga tgatccgtga ctgtgcaagt
     2881 ttccatggac tagacggtga atttatccga ttctgtattc agaagaaaga agataacgat
     2941 cgtttgctga atttattgac actctgatca taaatatttt tataaaacaa gaaatccctg
     3001 agaatgaatc ttttacaact cattctcagg gatttctata actcatctat ttattttact
     3061 gcgataactt caatctcgat cagtgctcct tttggtagag ctcctgcctg tacacaagat
     3121 cttgctggtt tgttatctcc aaatgcttct tcgtagattt cattgatttc tgcaaaatcg
     3181 ttgatatctg ctaagaacac tgttgttttg ataacattat ctaatgtaag atctgatgct
     3241 tttaatacag cacctaagtt cactaatacc tgtttggtct gtgctgcgac accttctttt
     3301 aattcctgtg tctgtggatc taatccgatc tgtccggatg taaatacgaa atctcctgct
     3361 tctactgcat gacaatatgg tcctactgct gctggagcat tctctgcatt gatgatcttt
     3421 gtatccatct tatcttcctg cctttctctt tggtcattta cttgatcact ttaatcgatg
     3481 ggtaatacct aaaccatgct tttcctaaga cttcatctct ggaaacataa tgggtctccc
     3541 attctctggc atcatttgaa ttatttctgt tatctcccat cacaaaatac ccgttctttg
     3601 gtactttata tggtccaaaa tcttctactg gttcttcttt gatatatggc tcactgagtg
     3661 ctttcttact tccattaata tatactttac cgtcttttac caacacagtc tctcctggaa
     3721 gtgctatcac acgcttaata aaccattctg tctcatcatc cggatatttg aagataatga
     3781 tatctccacg tttcggatcg ttaaaccaat aggatgttcg aagagcgatc agtttatcat
     3841 tcgtcatgat cgtattctcc atcgaaccac ttggaatatt cgcattgata attacaaaat
     3901 tcaagataaa ttctgtcaga agaatcgtta caacaataca tgcgatccag ctaaatacct
     3961 cacgcttgaa acttctcttc tgttctttat ctgcattttc tttgctcata ttgatctgtc
     4021 cttaataatt aatcctcaaa accacgtctg atgatatcat gtctttctgg tccgttagat
     4081 atcaaagtga tcggatatcc aatcttctct tctacgaact cgatgtactt acggcagttt
     4141 tctggaagat cttcgtagtt tttgattcca cggatatcag atttccatcc tggaagtact
     4201 tctaatactg gtttcgcttt ctcaagtttt cctgttgttg ggaaatctgt tgttacttct
     4261 ccatcaattt cgtatccaac acatacagga atctcatcta agtatccaag tacgtctaat
     4321 actgtaaatg caacatctgt tgttccctgc atacggcatc cgtatttggt tgctacaac
//
LOCUS       DAOMUE010000172         4353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5689_length_4353_cov_2.413215, whole genome shotgun sequence.
ACCESSION   DAOMUE010000172 DAOMUE010000000
VERSION     DAOMUE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5689_length_4353_cov_2.413215"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLC71_07805"
     CDS             complement(<1..188)
                     /locus_tag="PLC71_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRM02582.1"
                     /translation="MKDKNRGIICIILSAFSFALMALFLKLSGDLPAIEKSFFRNLVA
                     VVIAFIMIIRSKEKIQIQ"
     gene            complement(196..753)
                     /gene="efp"
                     /locus_tag="PLC71_07810"
     CDS             complement(196..753)
                     /gene="efp"
                     /locus_tag="PLC71_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRM02583.1"
                     /translation="MVTAGDFKNGLTIQFENNIYQIIEFQHVKPGKGAAFVRCKLKNI
                     KNGGVVEKTFRPTEKFENAHIERKEMQYLYNDGELFYFMDNETFDQIALGPDVIGDSL
                     KFVKENENVTIVSHAGNAFQVEPPISVELLVTECEPGEKGNTAQGATKPCIVETGAQV
                     MVPLFVNEGDTLKIDTRTGEYSSRV"
     gene            complement(818..2002)
                     /locus_tag="PLC71_07815"
     CDS             complement(818..2002)
                     /locus_tag="PLC71_07815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRM02584.1"
                     /translation="MHGFTQYTPTRIIFGKETQKQAGELAKSLKASKVFVVYGGGSVV
                     RSGLLDEVTASLEEAGLEYMTVGGVKPNPRLSFAREAVKKSIEFGTDFVLAVGGGSVI
                     DTSKAVAHGPANPDTDIWEFWSKKQTVKKSLPVGVVLTLAAAGSETSDSAVLTNEETK
                     IKRGLSTDFNRPAFAIMNPELTYTLPKYQVGCGIVDIMMHTLDRYFTQTENNELTDEI
                     AEGLLRTVIRNGAVAIKDSHDYNAMSEIMWAGSLSHNGITGLGAEKDFAVHQLGHELS
                     AKFDIAHGASLSAVWGAWATYVYDAKPARFAQYARRVWNVEEADDVKAAQEGIRKTVT
                     YFASLDMPTSFREAKEIGSKTDEEVKQMAHGCSYEGTRTIGSFKVCDENDIYEIYKLA
                     NK"
     gene            complement(2055..2837)
                     /locus_tag="PLC71_07820"
     CDS             complement(2055..2837)
                     /locus_tag="PLC71_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRM02585.1"
                     /translation="MSQLEEIRERLDLCDKKIVSVLEERMDIIKEIMVYKKENGLPIL
                     QPEQEKRQRKMLLTHVDENTYKDEILDIFTYIVENSRRIQAKTLFTHNILLIGFMGAG
                     KSTVSTYLSKILASPQVEMDEEIVKKEGMSINKIFEEYGETYFRNCETNLLIELQKKN
                     NQIISCGGGVALRDENVAEMKKNGKVVLLTASPEVILERVKDNDDRPLLRGNKNTEFI
                     SNMMEQRRPKYEAAADVVVNTDYKTVEEIAEEIVVKLTRDAD"
     gene            complement(2954..3841)
                     /locus_tag="PLC71_07825"
     CDS             complement(2954..3841)
                     /locus_tag="PLC71_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM02586.1"
                     /translation="MNLNHLYYFRALAKEEHYTRTADMLSITQPSLSHAISCLESELG
                     VKLFEKQGRNAKLTKYGALFLRYVEQSLDMLDEGKRVVTETSGTSGGFIDMGYIFTLG
                     SHFIPNLIKGYLEEKGKNHIHFSFGQGTTKKIVEELKDGKYDLAFSSYVEGEDELEFI
                     PVGQEELVLITPKDHPLAQKEEIDLLDTIEYDYVYFTKNSGLRPVIDSCFSRIKRQPT
                     IAYEGEEDSSVAGLVAAGFGIAIVPKIPLLDTMDVCVRPIISPEIERYIYLVLKKEKY
                     LTPVVKDFISFITDQSEFA"
     gene            3969..>4353
                     /locus_tag="PLC71_07830"
     CDS             3969..>4353
                     /locus_tag="PLC71_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dienoyl-CoA reductase"
                     /protein_id="HRM02587.1"
                     /translation="MAAKNFRKLFEPYTVKSMTLRNRIVMTPIGTNFAEHTGEVNERI
                     IDYYKQRAKGGTGLITIEAASVYSPQGASGSYQLRIDHDNYIPGLYRLCESIHDCGSK
                     ISILLNHAGSVANGKMTNIQPVSASD"
BASE COUNT         1203 a         1010 c          661 g         1479 t
ORIGIN      
        1 ttctgaatct gaatcttttc cttgctacga atgatcatga taaatgcaat gaccaccgca
       61 actaaattgc ggaaaaaact cttctcgatc gctggaagat ctcctgacag ctttaaaaac
      121 aatgccataa gggcaaagga aaaagctgac agtatgatgc atataattcc acgattctta
      181 tctttcataa caagattata ctcttgaaga atattctcca gttcttgtat cgatctttaa
      241 cgtatcacct tcgttaacga ataatggtac cattacctga gcacctgttt caacgataca
      301 tggttttgta gcaccctgtg ctgtgtttcc tttttctcct ggttcacatt ctgtaacaag
      361 taattctaca gagattggtg gctcaacctg gaatgcattt cctgcgtgag aaacgatcgt
      421 aacgttttcg ttttctttaa cgaattttaa agaatctccg ataacatctg gtcctaatgc
      481 gatctgatca aatgtttcgt tatccatgaa gtagaataat tctccatcat tgtacagata
      541 ctgcatttct ttacgttcga tatgagcgtt ttcaaatttc tctgttggac ggaatgtttt
      601 ctcaacaact ccaccgttct tgatattttt taatttacaa cgtacgaacg ctgctccctt
      661 tccaggtttt acatgctgga attcgatgat ctggtaaata ttattctcga actgaattgt
      721 taatccattc ttaaaatctc ctgctgtaac catattataa ttgtcctcct taaattatat
      781 ctttttttat tttatcttaa atctatagtt gaatcaacta tttatttgct aatttataga
      841 tttcgtatat gtcattctca tcacagacct taaaagaacc gatcgttctg gttccttcat
      901 aggaacatcc atgtgccatt tgcttcactt cttcgtctgt ttttgaacca atttctttcg
      961 cctcacgaaa acttgtcggc atatcaagtg atgcaaaata tgtaactgtt tttcggattc
     1021 cttcctgtgc tgccttcaca tcgtcagctt cttctacatt ccagactctt cttgcatact
     1081 gcgcaaatct ggctggtttt gcatcatata cataagttgc ccatgctccc cagacagcgg
     1141 ataaacttgc cccatgtgca atatcaaact tcgcactcag ttcatgtcct aactgatgca
     1201 ctgcaaagtc tttctcagct ccaagtcctg taattccgtt gtgtgaaaga cttcctgccc
     1261 acatgatctc actcatcgca ttataatcgt gtgaatcctt gatcgcaacc gcaccatttc
     1321 tgatcactgt acgaagcaga ccttctgcaa tctcgtctgt cagttcatta ttctctgtct
     1381 gtgtaaaata acgatccaat gtatgcatca taatatccac aatgccgcat cctacctgat
     1441 actttggtaa tgtatatgta agttcaggat tcatgatcgc aaatgccggg cggttgaaat
     1501 ctgtggaaag acctctcttg atcttcgtct cttcattcgt aagtactgct gaatcgcttg
     1561 tctcacttcc tgccgctgca agtgttaata caactccaac aggaagagat ttcttcactg
     1621 tctgtttctt agaccagaac tcccagatat ctgtatccgg attcgccggt ccatgtgcaa
     1681 cagcctttga ggtatcgatc acacttcctc cacctactgc caatacaaag tccgttccaa
     1741 attcaatgga tttcttcact gcttctctcg caaatgaaag ccttggattc ggcttcacgc
     1801 ctccaactgt catatattca agaccggctt cttctaatga agcagttacc tcatcaagta
     1861 atccgcttct tacaacactt cctccaccat agacaacgaa tactttggaa gctttcaggc
     1921 ttttcgcaag ttctcctgcc tgcttctgtg tctcttttcc aaaaatgatt cttgttggtg
     1981 tatactgggt aaatccatgc attcgctcat cctccatctg cccataaaca ggggcaaaac
     2041 ttgtatgtta ctgattaatc tgcatctctt gttaacttca caacgatctc ttctgcgatc
     2101 tcttccacag tcttataatc tgtgttcact acaacatctg ctgccgcttc gtactttgga
     2161 cggcgttgtt ccatcatatt agaaataaat tcagtattct tattcccacg taataatggt
     2221 ctgtcatcat tgtctttgac acgctctaag atcacttctg gagatgctgt taataaaacg
     2281 acctttccat tcttcttcat ctcagcaacg ttctcatcac gaagcgcaac tccaccacca
     2341 caggagatga tctggttatt cttcttctga agctcaatca aaaggtttgt ctcacagttt
     2401 ctgaaatatg tctctccata ttcttcaaaa atcttattga tagacattcc ttctttcttc
     2461 acgatctctt catccatctc tacctgtgga gatgcaagga tcttacttag atatgtagaa
     2521 acagtactct tacctgctcc cataaatccg atcaataaga tgttatgtgt aaataatgtc
     2581 tttgcctgaa ttcttctgct gttttctaca atataagtaa atatatcaag aatctcatcc
     2641 ttataggtat tctcatccac atgtgtgagc agcatcttac gctgtctctt ttcctgttct
     2701 ggctgaagga ttggcaatcc attctctttc ttgtagacca tgatctcttt gatgatatcc
     2761 attctctctt ctaatacaga gacaatcttc ttatcacaca gatctaatct ctctctaatc
     2821 tcttctaact gactcatctt cttgttcttt ccttttctat actaaatgtc cccatacttc
     2881 tcagataacg aagtgtggtc ttgttcagta tatcatacat tcctatattt tttcaattca
     2941 ttgcctctat aatttaggca aattcacttt gatctgtgat aaatgaaata aaatctttca
     3001 caaccggtgt cagatatttt tcttttttta gtacaagata tatatatcgt tcgatctctg
     3061 gtgatatgat cggacgaaca cacacatcca ttgtatcaag aagcggaatc tttggaacaa
     3121 tcgcaattcc aaatcctgct gcaacaagcc ctgcaacaga agaatcttct tccccttcat
     3181 atgcaatcgt tggttgcctt ttaatccttg aaaaacaact gtcgatcact ggccttaatc
     3241 cactgttctt tgtaaaatat acgtaatcat attcaattgt atccagaaga tcgatttctt
     3301 ccttttgagc caatggatgg tcctttggtg tgatcaaaac cagttcttcc tgaccaaccg
     3361 gaataaattc aagttcatct tccccttcca cataagaaga aaatgcaaga tcatatttcc
     3421 catcttttaa ttcttccacg attttcttcg tcgttccctg tccaaatgaa aaatgaatat
     3481 gattcttccc tttttcttcc agataacctt tgatcagatt tggaataaaa tgacttccca
     3541 aagtaaagat ataccccata tcaataaatc caccgctggt tcctgatgtt tctgttacca
     3601 ccctttttcc ttcatccaac atatccagcg actgctccac ataacgtaaa aacaatgctc
     3661 catatttcgt taattttgca tttctcccct gtttttcgaa taatttcact cctaattcac
     3721 tctcaagaca tgagatcgca tgacttaaac ttggctgcgt aatggacagc atatctgcag
     3781 ttctagtata gtgttcttcc ttagctaatg cccgaaaata gtacaaatga ttcaaattca
     3841 taatctattc cctttcaatt cataaaattt atttatggat agtataccat attcttcatt
     3901 gcatttatat atctactttg ctataattat ttttagaaaa ctattaagca aattggagga
     3961 tcataattat ggcagcaaag aatttccgta aattatttga accgtacaca gtaaaatcta
     4021 tgacattaag gaacagaatt gtcatgactc ccattggaac aaattttgct gagcataccg
     4081 gagaagtaaa tgagagaatc attgattatt ataaacagcg tgcaaagggc ggaacaggtc
     4141 tgatcacaat tgaagctgcg agtgtttatt caccacaggg agcaagcgga tcataccagc
     4201 ttcgtatcga ccacgataac tatattcctg gattatacag attatgtgaa agcatccatg
     4261 actgcggatc caagatcagc atcttattaa accatgctgg ttctgtagca aatggtaaga
     4321 tgacaaacat ccagccagta tctgcatctg ata
//
LOCUS       DAOMUE010000173         4335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5717_length_4335_cov_2.587850, whole genome shotgun sequence.
ACCESSION   DAOMUE010000173 DAOMUE010000000
VERSION     DAOMUE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5717_length_4335_cov_2.587850"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLC71_07835"
     CDS             <1..532
                     /locus_tag="PLC71_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM02588.1"
                     /translation="VNGHAYKADEYKTNNTNFALLVSKNFTKPFKEPIQYGKHIAQLS
                     NMLCDGKILVQRFGDFVRGRRTTEERLVRNNITPTLKDAVPGDLSLVFPYRIMKDIEE
                     MIYALDEVTPGMASDETLLYGVEVKFYSNKILTNKDFETNIKGLRAIGDGASVTRGLQ
                     QASANGLSVAKSILNK"
     gene            complement(688..1191)
                     /locus_tag="PLC71_07840"
     CDS             complement(688..1191)
                     /locus_tag="PLC71_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRM02589.1"
                     /translation="MKADLHCHTVLSDGAMRVDQIIPYAKRIGLDYIAISDHESTHSI
                     PEAVRLGKELGVHAIPAVEANAWHEETQTNVHILCYYPIDTDRLQHSLSKDLKKLSDA
                     VTKSYQSLMDEYPITMDQLYEVSKESTGVYYTHIMQVLSMMGYTGTPIGQLHNELFRA
                     GGPHKFP"
     gene            complement(1213..1635)
                     /locus_tag="PLC71_07845"
     CDS             complement(1213..1635)
                     /locus_tag="PLC71_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM02590.1"
                     /translation="MNDVSALFEIFPICQKFLLNTIDIPSMDLTKTQILILFALSGGR
                     SLNMSQLSSYLASSKEQATRAVSPLVKDEYVTRFRSDENRKMVYVKLTEKGNQLILQE
                     KALVKEYLSKRFESLSKEDQELFHQSLSNILTILKKME"
     gene            1792..3153
                     /locus_tag="PLC71_07850"
     CDS             1792..3153
                     /locus_tag="PLC71_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRM02591.1"
                     /translation="MSETTTQMQDFLTRQAKVCHDNCEIEKDLYKELGVKRGLRDVDG
                     VGVLAGLTNISCIQSSEMINGEKVPCEGKLFYRGYNIYELVDGFMKRGEFGFEEITYL
                     LLFGKLPDEKQLKEFREILAQSMTLPKNFTRDVILKAPSKDIMNTLTKSILTLASYDD
                     KASDISMENVLRQCLMLISVLPMMAVYGYHGYNHYVNDDSLYIHRPDPNCSMAENILR
                     MLRPDKSYTELEARVLDIALVLHMEHGGGNNSTFTTHVVTSSGSDTYSVIAAALSSLK
                     GPKHGGANIKVVEMMQDLRKNVDDISDESQVEEYLRKLLHKEAFDQKGLIYGMGHAVY
                     SISDPRAQIFKAFVKKLALAKEREKDFELYSMIETLAPKVIADERKIYKGVSANVDFY
                     SGFVYSMLDIPLELFTPIFAMARIVGWSAHRLEELTNVDKIIRPAYQSVQDEKEYIEM
                     EDR"
     gene            complement(3359..4186)
                     /locus_tag="PLC71_07855"
     CDS             complement(3359..4186)
                     /locus_tag="PLC71_07855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM02592.1"
                     /translation="MNIKCIALDLDRTTLNASGRLSDGNYNALCHTIENGVHIVIASG
                     RSFDTLPKDVLAVPGIEYAITSNGAAIYHIPTSTCLHEYKMTPASVECIIQIAKQHET
                     ALEVFIDGKAYALKAYVEDPVSYGTTPQAIPYIQSTRIPIDDIISFIREHIDHIDSMD
                     IVVSGEQQKQLIWNELKYNCDEIYITSSVSQLIEISHKDAGKHSGLRFIREYLNLKPE
                     ETAAFGDGDNDIDLLKEAGIGIAMENASSKCKDAATFITKHHDKDGVAYGITEFLKI"
BASE COUNT         1505 a          716 c          848 g         1266 t
ORIGIN      
        1 ggtaaatggt catgcataca aagcagacga atacaagaca aacaacacaa actttgcatt
       61 attagtatct aagaacttta caaaaccatt taaagagccg attcaatatg gaaaacatat
      121 cgcacagtta agcaatatgc tttgtgatgg caagatctta gtacagagat ttggagattt
      181 tgtgagagga agaagaacaa ccgaagaacg tctggtaaga aataacatta caccaacatt
      241 aaaagatgct gttccaggag atctttcact cgtattccca tatcgaatca tgaaagacat
      301 cgaagagatg atctatgcat tagatgaagt aacaccaggt atggcaagtg atgagacact
      361 tctttacggt gtagaagtga aattctattc taataagatc ttaacaaaca aagactttga
      421 aacaaatatc aaaggacttc gtgcgatcgg tgacggagct tctgtaacaa gaggattaca
      481 gcaggcatct gccaatggat taagcgttgc taagagtatc ttaaataaat aaaatatcaa
      541 ataaaaatat taaaacaaaa atccctgaca ggtttctaaa ccattaggga tttttctgtt
      601 tgagaatcct gcaaagaatg attcttcctt ataaaaaaat tattttttga atttaggatg
      661 tttttcatag aaagctttgt tcgcctgcta tggaaattta tgagggccgc cagcacggaa
      721 taattcgtta tgtaattgac cgatcggagt tccagtatat cccatcatgg aaagaacctg
      781 catgatatgt gtgtagtata caccagtact ttctttggat acttcataaa gctgatccat
      841 cgtgatcgga tattcatcca tcaatgactg ataagatttt gtgacagcat cggataattt
      901 ctttaagtct ttgcttaaag aatgctgaag ccggtcagtg tcaatcggat aataacataa
      961 aatatgaaca ttcgtctgtg tctcctcatg ccatgcgttt gcctcgacgg caggaatcgc
     1021 atgaacacca agctctttcc caagtcttac agcttccgga atggaatgtg ttgattcgtg
     1081 gtcagagatt gcaatataat caaggccgat acgtttcgca taaggaatga tctggtcaac
     1141 acgcatagca ccatcagata aaacggtgtg acaatgtaag tctgctttca tataaaaata
     1201 cctccaaaat atttattcca ttttctttaa gatcgttaaa atattagata atgactgatg
     1261 aaataattcc tgatcttctt ttgacagtga ttcaaaacgt ttggaaaggt attctttgac
     1321 aagcgctttt tcctgaagga tcagctggtt tcctttctct gtgagtttta cataaaccat
     1381 ttttctgttt tcatcacttc taaaacgtgt gacatactca tccttcacaa gtggagaaac
     1441 ggctcttgta gcctgttcct tagatgatgc cagataagaa gataactgtg acatattcaa
     1501 tgaacgacct ccagacagtg caaataagat aagaatctgt gttttggtaa gatccatgga
     1561 aggtatgtcg atcgtgttta aaagaaattt ctgacagatt ggaaaaattt caaaaagtgc
     1621 agaaacatca ttcaatgtaa atccctctct ttcgatgttt ttctaactaa aagtatacca
     1681 ctttatgaat aaaaatcaat aatcaacaaa aatcaatatt tgacaaaagt caaataaaaa
     1741 gataaaatag gagaaacact ataaaatgac cttgatcaaa ggaggtaacc tatgagtgaa
     1801 acaacaacac agatgcagga ttttctgaca cgccaggcga aagtctgcca tgataattgt
     1861 gagatagaga aagatctgta caaagagctg ggagtgaaac gaggactcag agatgtcgat
     1921 ggagtcggcg ttcttgcagg tcttacgaat atatcctgta ttcaatccag tgagatgatc
     1981 aatggagaga aagtaccttg tgaaggaaag ttattttata gaggatataa tatatatgaa
     2041 ttagtcgatg gctttatgaa gagaggcgaa tttggatttg aagaaatcac ctatctttta
     2101 ctatttggaa aactgccgga tgaaaaacaa ttgaaagaat tcagagaaat tttggcacag
     2161 agtatgacac ttccaaagaa tttcacaaga gatgttattt taaaggcacc atcgaaagac
     2221 attatgaaca cgttgacaaa gagcatttta acactggcat cgtatgatga taaagcatct
     2281 gatatttcta tggaaaatgt attaagacaa tgtctgatgt tgatcagtgt tttgccgatg
     2341 atggcagttt atggatatca tggttacaat cattacgtca atgatgacag cctgtacatc
     2401 catcgtccag atccaaactg ttccatggca gaaaatattt taagaatgct gcgtccagac
     2461 aaatcttata cagaattgga agccagagtg ttagatattg cactcgttct tcatatggaa
     2521 catggaggag gaaataactc aacatttaca acccatgtcg tgacatcttc aggatcagat
     2581 acatattcag tgatcgcagc tgcactttca tcattgaaag gtcccaaaca tggaggagcg
     2641 aatatcaaag ttgttgagat gatgcaggat ttaagaaaaa atgtggatga tatcagtgat
     2701 gaaagtcagg tagaagaata cttaagaaaa ctgctgcaca aagaagcatt tgaccaaaaa
     2761 ggactgatct atggaatggg gcatgcagta tatagtattt cagatccaag agcgcagatt
     2821 ttcaaagctt ttgtaaagaa actggccctt gcaaaagaaa gagaaaaaga tttcgaactc
     2881 tattctatga ttgaaacatt ggcaccaaaa gtaatcgcag atgaacgaaa aatctacaaa
     2941 ggggtcagtg caaatgtcga tttctacagt ggatttgtgt atagtatgtt agatatccca
     3001 ttggaattgt tcacaccgat ctttgcgatg gcaagaatcg ttggatggag tgcccatcgt
     3061 ttggaagaat taacaaatgt ggataagatc attcgtcctg cgtatcagag tgtacaagat
     3121 gagaaagaat acattgagat ggaagatcga taaaaaagag ggattccctc tttttttatg
     3181 caaaaatata agcttataaa atgaggaaaa ccggagacga ggagagtctc cggtattatg
     3241 aaaaagtatg aaaaaaagtt ttagctaagt aagtttgttt gttacttgct atggttttag
     3301 tatactcagt aaatgtgaaa gaattgtgtc taaaaaatga tgaatttatg ttttttggtc
     3361 atattttgag aaattcagtg attccatatg caactccgtc cttgtcatga tgttttgtga
     3421 taaaggtcgc agcatcctta cattttgaag aagcattttc catggcaatt ccgataccag
     3481 cttcttttaa cagatcaatg tcattatcac catcaccaaa ggcagcggtt tcttcaggtt
     3541 ttagattcaa atattcccga ataaaacgaa gtccggaatg ttttccggca tctttatgag
     3601 aaatctcgat cagctgcgaa acagaagaag taatatagat ctcatcacaa ttatatttaa
     3661 gttcattcca aatgagctgc ttttgctgct cgccagagac aacaatatcc atactgtcaa
     3721 tatgatcaat atgttctcga ataaaagaaa taatatcatc aataggaatt cttgtagatt
     3781 ggatgtaagg aattgcttgt ggagttgtac cataggaaac aggatcttcc acataagctt
     3841 tcagggcata agctttgcca tcaataaaaa cttcaagggc agtctcatgc tgtttagcaa
     3901 tctgaattat acattcaacg gaagcaggtg tcatcttata ttcatgaaga caggtgcttg
     3961 taggaatatg gtaaatggca gcaccattag aagtaattgc atattcaatt ccaggcacag
     4021 caagaacatc tttaggcagc gtatcaaaag aacgtccact tgcgatcaca atgtgaacac
     4081 cattttcaat tgtatgacat aaagcattat aatttccgtc agaaagccgt ccacttgcat
     4141 ttaatgttgt acggtcaaga tcaagtgcaa tacattttat attcataaga aacctccaga
     4201 tttcgcattt gttatacata gtcgatattg tgtaatatat tttacacata tgaaaatatt
     4261 gtatcttttc tttgaaacaa ttacaatata cgaaaggagg agaaataaaa atgggaatga
     4321 caacattatg ctata
//
LOCUS       DAOMUE010000174         4281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5824_length_4281_cov_2.284666, whole genome shotgun sequence.
ACCESSION   DAOMUE010000174 DAOMUE010000000
VERSION     DAOMUE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5824_length_4281_cov_2.284666"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLC71_07860"
     CDS             <1..615
                     /locus_tag="PLC71_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRM02593.1"
                     /translation="VSKRQYRKAPHLVDTNYDALEVLVHDIKPGSIDRIVTDQEEVGE
                     QLKEKGYEVFLCSYMAGDIERRYRFRHYLSEVFKRKIFMKSGGFLYIEQTEAMAVIDV
                     NTGKSIGKKNQEAHIKKINLEAAKEAARQIRLRNLSGIIMIDFIDMKSKDDEKELLQV
                     MQHYLNGDSKKAVAVDITKLGIMEITRKKEKNPIFRQISIDILK"
     gene            708..1016
                     /gene="rplU"
                     /locus_tag="PLC71_07865"
     CDS             708..1016
                     /gene="rplU"
                     /locus_tag="PLC71_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRM02594.1"
                     /translation="MYAIIATGGKQYKVSEGDVIKVEKLDAEVGAKVTFDNVLLVGGD
                     TVKVGTPTVDGAKVEASVVSEGKGKKVIVYKYKRKTGYHKKNGHRQLFTQVKIDSIVG
                     "
     gene            1035..1361
                     /locus_tag="PLC71_07870"
     CDS             1035..1361
                     /locus_tag="PLC71_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HRM02595.1"
                     /translation="MTKITFYQRVDGTFQGFHSQDHAGYSAEGQDIVCAAVSALVINF
                     VNSLDELTDDHYQIDVNQEDAEIDVVFTEELSEEGSLLLRSLILGLTSVEEEHGQYLD
                     VIFKEV"
     gene            1367..>1441
                     /locus_tag="PLC71_07875"
     CDS             1367..>1441
                     /locus_tag="PLC71_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRM02596.1"
                     /translation="MMKMNLQFFAHKKGMGSTKNGRDSE"
     assembly_gap    1442..1451
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1452..1646
                     /gene="rpmA"
                     /locus_tag="PLC71_07880"
     CDS             <1452..1646
                     /gene="rpmA"
                     /locus_tag="PLC71_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRM02597.1"
                     /translation="GAKRADGQFVKAGNILYRQRGTKIHPGVNVGRGGDDTLFALVDG
                     IVRFERKGKDKKQCSVHPVA"
     gene            1787..3070
                     /gene="obgE"
                     /locus_tag="PLC71_07885"
     CDS             1787..3070
                     /gene="obgE"
                     /locus_tag="PLC71_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRM02598.1"
                     /translation="MFADRANIIIRSGKGGDGHVSFRRELYVPNGGPDGGDGGRGGDV
                     IFVVDEGINTLSDYRHRRKFKAGDGQEGGRRKCHGADGEDIILKVPAGTVVKDKESGK
                     VILDMSNKKEPVVLLKGGRGGKGNQHYATPTMQAPKYAQPGGKAQELEVMLELKVIAD
                     VGLVGFPNVGKSTFLSRVTNANPKIANYHFTTLNPNLGVVDMDGSKGFVIADIPGIIE
                     GASEGVGLGFEFLRHIERTKVMIHMVDGASVEGRDPIVDIHAITDELKKYNKEILEKP
                     QVIAANKMDAMSETDRETVIDLLKEEFEPEGIKVFPISAVSGEGVKELLWHVQGLLDE
                     LPEGATEFEQEYELNFGEEEGGIFIGHPEEGLYTVEGPKVERMLGYTNLESERGFDFF
                     QKFMKENGVIERLTEMGIEEGDTVRLYNLEFDYYR"
     gene            3093..3392
                     /gene="yhbY"
                     /locus_tag="PLC71_07890"
     CDS             3093..3392
                     /gene="yhbY"
                     /locus_tag="PLC71_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HRM02599.1"
                     /translation="MTSKQRAYLKSLAMKIDPIYNIGKGSISPELVQGVRDALEAREL
                     IKIGVLQNCMDDPKELANVLAERTRSQVVQVIGKKIVLYKESKNHKRIELPKAGK"
     gene            3408..4031
                     /gene="nadD"
                     /locus_tag="PLC71_07895"
     CDS             3408..4031
                     /gene="nadD"
                     /locus_tag="PLC71_07895"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRM02600.1"
                     /translation="MKKIGILGGTFNPIHHGHLILGQAAKEEFGLDEILVMPTKNPAY
                     KKISGGVSEKNRVDMIKLAIRDFPYFKFSDIELKREGTTYTVDTLRELTKQDTDCRYY
                     FIMGADSLYQIETWKDPGQIFTMADILVATRNDSRSALDAQIDYLEEKYDGKIYHLSS
                     PSIEISSNDIRKRCSNGSSIHFFLPEDVIDYIERNDLYGSTADRRKA"
     gene            4000..>4281
                     /locus_tag="PLC71_07900"
     CDS             4000..>4281
                     /locus_tag="PLC71_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM02601.1"
                     /translation="MEVQQIEEKLKTALKPSRYRHTLGVAYTASCMAMVFGVDVHKAY
                     RAGLLHDCAKGFSMEEQRALCKRYNISLEGTLTKSPQLMHQEIAPFLASD"
BASE COUNT         1507 a          636 c         1030 g         1098 t
ORIGIN      
        1 gtatctaaaa ggcaataccg caaagctcct catcttgttg atacaaatta cgatgcattg
       61 gaagtgctgg ttcatgatat caaaccagga tcgatcgatc gtattgtgac agatcaggaa
      121 gaagtagggg aacagctgaa agaaaaaggt tatgaagtat tcttatgttc ttatatggca
      181 ggagacattg aacgaagata tcggtttcgt cattatcttt cagaggtatt taaacgtaaa
      241 atatttatga agtctggagg atttctgtat atcgaacaga cagaggcaat ggcagtcatt
      301 gatgtgaata cggggaaatc cattggaaag aagaaccagg aagcacatat taagaaaata
      361 aacttagaag cagccaaaga agcagcaagg cagatccgct taagaaattt atcagggatc
      421 atcatgattg attttattga tatgaagtcc aaagatgatg agaaagaatt attacaggta
      481 atgcagcatt atctaaatgg ggattctaag aaagcagttg ctgtagatat tacaaagctc
      541 ggaattatgg agatcacaag aaaaaaagaa aaaaatccaa tttttcgaca aataagtatt
      601 gacatattga aatagtgctg ttattatata tgagtatgcc gcacagcgag gttaaagaat
      661 gcgagaatca ccaaagctgg cgagatttga taataggagg tgccgtgatg tacgcaatta
      721 tcgcaacagg tggaaaacag tataaagtaa gcgaaggcga cgtgatcaaa gttgagaaac
      781 ttgacgctga agtaggagct aaagtaacat ttgataatgt acttttagtt ggtggagata
      841 ctgttaaagt tggaacacct accgttgatg gtgcgaaagt tgaagcttct gtagtaagcg
      901 aaggaaaagg taagaaagtc atcgtttaca aatacaagag aaaaactgga taccataaga
      961 aaaacggtca tagacagtta tttacacagg ttaagatcga tagtatcgtt ggataatatc
     1021 ctgaggatag agttatgaca aagataacat tctatcagag agtcgatgga acctttcaag
     1081 gtttccactc acaggaccat gcaggttaca gtgcggaagg acaagatatt gtctgtgccg
     1141 cagtgtctgc tctggtaatt aattttgtga attctctgga tgaactgaca gatgatcact
     1201 atcagattga tgtgaaccag gaagacgcag aaattgacgt agtatttacg gaagagcttt
     1261 ccgaagaggg gagcttgtta ttaagatcac tgatccttgg tctgacttca gtagaagaag
     1321 aacatggaca gtatttagat gtaatcttta aggaggtgta acaaaaatga tgaaaatgaa
     1381 ccttcaattt ttcgctcata aaaaaggaat gggttctaca aagaacggta gagattccga
     1441 annnnnnnnn nggtgcgaaa agagcagacg gacagtttgt aaaagctgga aatattcttt
     1501 acagacagag aggaacaaag attcatccag gcgttaacgt aggacgcggt ggtgacgata
     1561 cattattcgc attagttgat ggtattgttc gcttcgaaag aaaaggtaaa gataagaaac
     1621 agtgttctgt acatccagta gcataattta attgcataca ggaacagatc atttgagatt
     1681 cagtaattac ggcttcaaat acatatttga ggccgttttt cattataaga taatgaaata
     1741 tttttgtgac tggaagttgt ttttttgaca cagaaaggaa aagacaatgt ttgcagatag
     1801 agctaatatt ataatcagat ccggaaaagg tggagatggc cacgtaagtt tccggaggga
     1861 actttatgta ccaaacggtg gaccagatgg tggagacggc ggccgtggcg gtgatgtgat
     1921 ctttgttgta gatgaaggaa tcaatacttt atctgattac cgtcacagaa gaaaattcaa
     1981 ggcaggagat ggtcaggaag gtggcagaag aaaatgccac ggtgctgatg gagaagacat
     2041 catcctgaaa gttccggcag gaaccgttgt aaaagacaag gaatcaggga aagttatcct
     2101 tgatatgtct aataagaagg aaccagttgt acttttaaaa ggcggtcgtg gtggtaaagg
     2161 aaaccagcat tatgcaactc caaccatgca ggcaccaaaa tatgcccagc caggtgggaa
     2221 agcgcaggaa ctcgaagtta tgcttgagtt aaaagttatt gctgatgtag gtttggttgg
     2281 attcccaaat gtaggaaaat ctacattttt atcaagagta acaaacgcaa atccaaagat
     2341 cgcaaactat catttcacaa cattaaatcc aaatcttgga gttgtggata tggatggaag
     2401 caaaggattt gttattgcag atatcccagg aatcatcgag ggggcgtctg aaggtgttgg
     2461 tcttggattt gaatttttac gtcatatcga acgtacaaaa gttatgatcc atatggtaga
     2521 tggagcttca gtagaaggaa gagatccgat cgttgatatc catgctatca cagatgaact
     2581 taagaaatac aacaaagaga ttctagagaa accacaggtg atcgcagcga acaagatgga
     2641 tgctatgtct gagacagaca gagagactgt gatcgattta ttaaaagaag agtttgaacc
     2701 agaaggaatc aaagtattcc ctatttctgc ggtaagcggg gaaggtgtta aagaattatt
     2761 atggcatgta cagggattat tagatgaatt accagaaggt gcaacagaat ttgaacagga
     2821 atacgaatta aacttcggag aagaagaagg tggaatcttt atcggtcatc cagaagaagg
     2881 attatatacc gtcgaaggac cgaaagtaga gagaatgctt ggatatacca atctggaatc
     2941 tgagcgtgga tttgacttct tccagaaatt catgaaagaa aacggtgtca ttgaacgttt
     3001 aacagaaatg ggaatcgaag aaggagacac tgtacgtttg tataatctgg agtttgacta
     3061 ctatagatag atataattga atggaggata acatgacaag caaacagaga gcttatttaa
     3121 agagccttgc aatgaagatc gatccgatct ataacattgg aaaaggaagt atttcaccag
     3181 aacttgtaca gggtgtcaga gatgctttag aagcaaggga attgatcaag atcggtgttc
     3241 ttcaaaactg tatggacgat ccaaaagaac tggcaaatgt tcttgcagag agaacaagat
     3301 cccaggttgt acaggttatt ggtaagaaga tcgtattata taaggaatca aagaatcata
     3361 agagaattga actgccaaaa gcagggaaat aatatcggag gacagccatg aagaagatag
     3421 gaattcttgg cgggacattc aatccgatcc atcatggaca tctgattctt ggacaggcag
     3481 cgaaagaaga gtttggattg gatgagattt tggttatgcc aacaaagaat ccggcatata
     3541 aaaagatcag tggcggtgtc agtgagaaaa acagagtcga tatgatcaag ctggcgatca
     3601 gggattttcc gtatttcaaa tttagtgata tcgaattaaa acgtgaagga acaacgtaca
     3661 cagttgatac gttaagagaa cttacaaaac aggatacaga ttgcagatat tacttcatca
     3721 tgggagcaga ttcgctttat cagatcgaga catggaaaga tccaggacag atttttacca
     3781 tggcagatat attagtagct acaagaaatg actcaagaag tgctctggat gctcagattg
     3841 attatctgga agagaaatac gatggcaaga tttatcattt aagcagtcca agcattgaga
     3901 tttcatccaa tgatatcaga aaaagatgta gcaatggatc aagtattcat ttcttccttc
     3961 cagaagatgt gattgattat atagaaagaa atgatttata tggaagtaca gcagatagaa
     4021 gaaaagctta aaacagcgtt aaaaccatcc agataccggc atacactagg agtagcttat
     4081 acagcttcct gtatggcaat ggtgtttgga gtggatgttc ataaagcata tcgcgcaggt
     4141 cttttacatg actgtgccaa aggcttttca atggaggagc aaagagcgtt atgcaaacga
     4201 tataatatca gtctggaggg aactttaacc aaaagtccgc agctgatgca tcaagagatc
     4261 gctccatttc ttgcaagcga t
//
LOCUS       DAOMUE010000175         4276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5833_length_4276_cov_3.312248, whole genome shotgun sequence.
ACCESSION   DAOMUE010000175 DAOMUE010000000
VERSION     DAOMUE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5833_length_4276_cov_3.312248"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PLC71_07905"
     CDS             complement(<1..1088)
                     /locus_tag="PLC71_07905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM02602.1"
                     /translation="MKKLAKKLLCLTAVLALTLSLFTGCKSKNEKTLFEYAGQEVTFQ
                     EAHVYARIMQYQAEAQYGAYFGDSMWSMQVGTDSKGKKITMQQSVKDSVINQLKQIKV
                     LAAHADDYNVKLTKSEKKQIKESVTAFAKDSTGKKVMKKTEADKDMIQKLYEESTIAS
                     KVMQAIIKKANVTVTDDEAKTVKVYKLVFTTKTTDSKTGKEKDMTAAEKKDQLKKAKS
                     ALKMIKKGQSISSVAKKFSVNSDNEESYTKGKATLGTKFETAAAKLKKNQVSGVVELD
                     DAYVIIKMLNPNDTTAAASNKSTLLQEKQQAAYEKVYKKWTKDADKKWDDKKSVDQDL
                     WKEVKFKYKATTASTATTTTTSTTASKTT"
     gene            complement(1152..>4276)
                     /gene="mfd"
                     /locus_tag="PLC71_07910"
     CDS             complement(1152..>4276)
                     /gene="mfd"
                     /locus_tag="PLC71_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRM02603.1"
                     /translation="NHKMTFKMGETLEEATIAEEFVARGYEKAPFVEAPGEFAVRGGI
                     IDIFPYTQESPYRIELWGDEIDSIRSFDKESQRSIEEVDTLTIYPASEIILNQPRIEH
                     GLRNMEEDYEKLSQKFKKEKKYDQEARLRKEMDRVREELRELHMLIGVEGYLPYFYEN
                     TECILDYFPEESMIYMDEPKHIRERADAFYLEFSESMKSRLEGGYLLPKQANTVTDYE
                     SILYQIEKHKMLCYSILSAEINDFRVARTLFMDTKSIQSYNNSFEQLVKDLTKYQSKD
                     YRIVVASPSVTRAKRLSSDLRENGLVVTYDKDLKYGVEAGQIVVTAGKLLTGIEYPAA
                     KWVLISEGDIFKGREEKKRRKKEKKKQGEKIRSFADINIGDYVVHEKHGVGIYRGIEK
                     ITTDGVEKDYISIEYQGGDNLFILASALDQIAKYASANARKPKLHKLGGNEWKKTKTR
                     VKGQVKDIAEELVQLYALRQAKEGYVYDKDTVWQKEFEELFPYDETQDQLNAIDDTKR
                     DMESKKIMDRLICGDVGYGKTEVAIRAAFKAVDNGKQVAYLVPTTILAQQHYNTFAQR
                     FHDYPITVRMMSRFCTAKEQKETIEGLKNGTVDVVIGTHRLLSKDMQYKNLGLLVIDE
                     EQRFGVTHKEKIKTMKKDVDVLSLSATPIPRTLHMSLIGIRDMSVLEEPPHDRRAIQT
                     YVMEYNEELVKEAVYREMTRGGQVYYVYNRVNNIAEVTAELQRLLPDAKVAFAHGQMK
                     ERELEEIMMGFMNHEIDVLVSTTIIETGLDIPNVNTMIIHDANQLGLSQLYQLRGRVG
                     RSNRNAFAFLMYKKNTLLKETAEKRLQAIREFTDLGSGYKIAMRDLEIRGAGNLLGQA
                     QSGHMEAVGYDLYCKMLNEAVLRLKGELKDEDEFDTTLDLDINAFIPSTYVYNEYQKL
                     ELYKRISSIESKDEMEDMTDELIDRFGEMPKAVYNLLYVAYLKSLAHHAYMTDVKQKG
                     EKVSFEMKPDAAVDVEKIPAILEEYKRELSFQAGKNPTFVLDLSKTKKVKQLEKIEHC
                     VNSLNSLIIR"
BASE COUNT          965 a          974 c          728 g         1609 t
ORIGIN      
        1 gttgtcttac ttgctgtcgt tgatgttgta gtagtagttg ctgtagatgc tgttgtagct
       61 ttatatttga acttaacttc tttccacaga tcctgatcta cagacttctt gtcatcccac
      121 ttcttgtcag catcttttgt ccacttcttg tagactttct cgtatgctgc ctgctgtttt
      181 tcctgaagca gtgtggattt gtttgatgca gctgctgttg tgtcgtttgg atttaacatc
      241 ttgatgatca cgtatgcatc gtctaattct acgactccgc ttacctggtt cttcttaagt
      301 tttgctgctg ctgtttcgaa ttttgttcct aatgtggctt ttccttttgt ataggattct
      361 tcgttgtcag agtttacgct gaacttctta gctacagagc tgatactctg gcctttcttg
      421 atcatcttta atgcagattt tgctttcttt agctgatctt tcttctcagc ggctgtcatg
      481 tctttttctt ttcctgtctt gctgtctgtt gtctttgttg tgaagacaag tttgtagact
      541 ttgacagttt tggcttcgtc atctgttact gtaacgtttg ctttcttgat gatcgcctgc
      601 ataactttac ttgcgatcgt gctttcttcg tacagtttct ggatcatatc tttatctgct
      661 tctgtcttct tcatgacttt cttacctgtt gagtcttttg cgaaggcagt tacgctttct
      721 ttgatctgct tcttctcaga ttttgttaac ttcacgttgt agtcgtctgc gtgtgctgct
      781 aaaactttga tctgttttaa ctggttgata acgctgtctt tgacagactg ctgcattgtg
      841 atcttctttc ctttgctgtc tgttccgacc tgcatagacc acatgctgtc tccgaagtat
      901 gctccgtact gagcttctgc ctgatactgc atgattcttg catatacatg tgcttcctgg
      961 aatgttactt cttgtcctgc atattcaaat aatgtcttct cattcttgct tttacatcct
     1021 gtaaataaag aaagagttaa cgcaagaact gcagttaaac atagcaactt ctttgctaat
     1081 ttcttcattt cattttctcc ttcatattat gattatatta ggctaatgtc tggcgatatt
     1141 atagcacaaa cttatcggat tatcaatgaa ttgagcgagt tcacacaatg ttcaatcttt
     1201 tctaactgct tcactttctt tgtctttgac agatccagta caaatgtcgg attcttgcct
     1261 gcctggaagg ataattcacg tttgtattct tctaatatcg ctgggatttt ttctacatcc
     1321 acggctgcat ctggtttcat ttcaaatgag actttttctc ctttttgttt cacatctgtc
     1381 atataagcgt ggtgtgctaa tgatttcaaa tatgcaacat ataacagatt gtagactgct
     1441 ttcggcattt ccccaaaacg gtcaatcaac tcatctgtca tatcttccat ttcatctttg
     1501 gattcaatgg atgaaatacg tttgtataat tccagttttt gatattcatt atatacatac
     1561 gttgatggaa taaatgcgtt gatatcaaga tcgagtgttg tgtcaaattc atcctcgtct
     1621 tttaattcgc cttttaatct caagactgct tcattcagca tcttgcaata cagatcgtat
     1681 ccaactgctt ccatatgacc ggattgtgcc tgtcccaaaa gatttcctgc tccgcggatc
     1741 tccagatctc gcattgcgat cttataaccg gatcccagat ctgtaaattc ccggatcgcc
     1801 tgtaatcgtt tttctgcggt ttcttttaac agtgtgtttt ttttgtacat caaaaatgca
     1861 aatgcatttc gattcgatct tccaacacgt cctcttaact gatacaactg ggacaaccca
     1921 agctggttgg catcgtgaat gatcattgta tttacattcg gaatatctag tcctgtctcg
     1981 atgatcgttg tagataccag gacatcgatc tcatggttca taaatcccat catgatctct
     2041 tccagttcgc gttctttcat ctgtccatgt gcaaatgcaa ctttcgcatc tggaagcaat
     2101 ctctgtaatt cggctgtcac ttctgcgata ttattgacgc ggttatacac ataatatacc
     2161 tgcccgccac gtgtcatttc gcggtagacc gcttctttga ccagttcttc attatattcc
     2221 ataacatatg tctggatcgc tcttcggtca tgcggtggtt cttctaatac actcatgtca
     2281 cggattccga tcagactcat atgcagggtt cttggaattg gtgtagcaga taaggataag
     2341 acatcgacat cttttttcat tgtcttgatc ttttctttat gtgtcactcc aaaacgctgt
     2401 tcttcatcaa ttacgagcag tcccagattc ttatactgca tgtctttgga caataagcga
     2461 tgcgttccga tcacgacatc taccgttcca tttttgagtc cttcaatcgt ctctttctgc
     2521 tcttttgctg tacaaaatct ggacatcatg cgtacggtga tcgggtaatc atgaaatctc
     2581 tgtgcaaatg tattgtaatg ctgctgtgca aggatcgtgg ttggtacgag atacgccacc
     2641 tgttttccat tgtctactgc cttaaatgct gcacggatcg ctacttctgt cttgccatac
     2701 ccgacatctc cacagatcag tcggtccatg atcttcttgc tttccatatc ccttttcgta
     2761 tcatcgatcg catttaactg atcctgcgtt tcatcgtatg gaaataattc ttcaaactct
     2821 ttctgccata cagtgtcctt gtcatagaca tatccttctt ttgcctgtct tagtgcatat
     2881 aactgcacga gttcttccgc aatatctttg acttgacctt tgacacgtgt tttcgtcttc
     2941 ttccattcat ttccacctaa tttatgaagt ttcggctttc ttgcatttgc acttgcgtat
     3001 tttgcgatct ggtctagggc tgatgctaaa ataaataaat tatcaccgcc ctgatattca
     3061 atactgatgt agtccttttc aaccccatct gttgtgatct tttcgattcc gcggtaaata
     3121 ccgactccat gtttttcatg aacaacgtaa tcaccgatat tgatatctgc aaaacttcgg
     3181 atcttttctc cttgtttttt cttctctttc tttcggcgct ttttttcttc tcttcctttg
     3241 aaaatgtctc cttctgagat caatacccat ttggctgccg gatattcaat tcctgtcaga
     3301 agctttcctg ctgtgacaac aatctgccct gcttccactc catatttcaa gtccttatca
     3361 tatgtcacaa ccaaaccatt ttctctcaga tcactgctta gacgtttcgc tcttgtaaca
     3421 gatggagaag caacaacgat cctgtagtcc ttactctgat atttggtaag atctttgact
     3481 aactgctcaa aactgttgtt ataagactga atggattttg tatccataaa taaggttcta
     3541 gcaactctaa aatcattgat ctcagcagat aagatcgaat aacatagcat cttatgtttc
     3601 tcgatctgat ataaaatact ctcataatct gtcaccgtat ttgcctgttt aggcagaaga
     3661 tacccacctt ccagacggct tttcatactc tctgagaatt ccagataaaa tgcatctgca
     3721 cgttctctga tatgttttgg ttcatccata tagatcatgc tttcttctgg aaaataatct
     3781 aagatgcatt ctgtattttc ataaaaatat gggagataac cttctactcc gatcaacata
     3841 tgaagttccc ttaattcctc acgaacacgg tccatttctt ttcttaggcg ggcttcctga
     3901 tcatattttt tttctttttt aaacttctgt gataacttct cataatcttc ttccatattt
     3961 cgaagaccat gttcaatcct tggctggttt aaaatgatct ctgatgccgg ataaatggtc
     4021 agagtatcga cttcttcgat ggatctttgg ctttctttat caaaacttcg gatcgaatca
     4081 atttcatctc cccatagttc gatacgatat gggctttcct gcgtgtatgg gaaaatatca
     4141 atgattcctc ctcgcacagc aaactctcct ggagcctcaa caaatggtgc tttttcgtat
     4201 cctctggcaa caaattcttc tgcgatcgtt gcctcttcca gtgtctctcc cattttaaac
     4261 gtcatcttat gattct
//
LOCUS       DAOMUE010000176         4211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5933_length_4211_cov_2.017084, whole genome shotgun sequence.
ACCESSION   DAOMUE010000176 DAOMUE010000000
VERSION     DAOMUE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5933_length_4211_cov_2.017084"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PLC71_07915"
     CDS             complement(<1..955)
                     /locus_tag="PLC71_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRM02604.1"
                     /translation="MNALALGNSGRGVNESSLDAARKIYEVRTKVDQFFDGYGAEQVV
                     FTSGITESLNTVIKGSLNHGDHVITTFMEHNSVLRPLYEMERQGVCLTITSPDVGDIK
                     QAITKDTKMIVMTHASNVTGEMFDIQSVGKLCREKGILFVVDTAQSAGVIPISMKEDN
                     IDILCFTGHKGLMGPQGIGGICIRKGVEIRPLKTGGTGILSFSKTQPGVLPQALEAGT
                     LNGIGIVGLGAAIDFINTYGIDKIREQEMNLIEIFYKKIQKIQGIKIYHEKQLDLTKQ
                     TAICSINLEEYDSGSVSDELMERFQIQTRSGAHCAPLVHEHF"
     gene            complement(1100..1888)
                     /gene="yqeB"
                     /locus_tag="PLC71_07920"
                     /pseudo
     CDS             complement(1100..1888)
                     /gene="yqeB"
                     /locus_tag="PLC71_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
     gene            complement(1898..2503)
                     /gene="yedF"
                     /locus_tag="PLC71_07925"
     CDS             complement(1898..2503)
                     /gene="yedF"
                     /locus_tag="PLC71_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HRM02605.1"
                     /translation="MKQIDERGNACPIPVVHTKKALESMDGETKLIVLVDNEPAVQNV
                     TRFAEGKNCEVKSEKISDNEFKIEIMAENYTPADEEEEIVCAPSAKDDFIIAVSSNEM
                     GQGEKALGQTLIKAFLFAVTKQDKLPSKVLFYNSGAYLTCEDSDSLEDLKTLEAEGVE
                     IYTCGTCLNFYDITDKLAVGSITNMYDIVEMMEKAGKVIKP"
     assembly_gap    2545..2583
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2584..2744)
                     /locus_tag="PLC71_07930"
     CDS             complement(<2584..2744)
                     /locus_tag="PLC71_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HRM02606.1"
                     /translation="MRKKELKTVITFETTTQAIGMESACKKHQIAGRLIPVPKEITAG
                     CGMAWMTPL"
     assembly_gap    2868..2877
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2878..3114)
                     /locus_tag="PLC71_07935"
     CDS             complement(<2878..3114)
                     /locus_tag="PLC71_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM02607.1"
                     /translation="MKSYLPKRFDFRVRVFMEEPDFGKGVAQLLSLTREKGSLSAAYK
                     SMGMAASKAWKILNRAEADLGVKLVERKSGGKQGG"
     gene            complement(3117..4151)
                     /locus_tag="PLC71_07940"
     CDS             complement(3117..4151)
                     /locus_tag="PLC71_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRM02608.1"
                     /translation="MKLVDTKDAVGTVLCHDITQIIKGVTKDARFRKGHVVKEEDIPV
                     LLSLGKDHLYVWEAGDDMLHENDAARILCNLCKNEGMKESEVKEGKIELSAEYDGLLK
                     IDKEKLFTVNSFGQMMIATRSGNTPVKAGDKLAGTRIIPLVIEKDKMQEVKDRVGDTP
                     ILSLLPYKKKKAVILATGNEVYYHRIEDTFTPVVAEKIKEYGAEVTSTKQLPDDNELL
                     TKTILEAIENGAEMVLCTGGMSVDPDDKTPLAIKNTGADIISYGAPVLPGAMFLLAYY
                     KKGDQIIPIMGLPGCVMYSKRTVFDLILPRIMADDKVSAEELAHLGEGGLCLGCKVCT
                     YPNCGFGKGW"
BASE COUNT         1054 a          975 c          642 g         1491 t
ORIGIN      
        1 cgaaatgttc atgtaccaat ggtgcacaat gagcacctga ccttgtctgt atctggaaac
       61 gttccattaa ttcatcactg acacttccag aatcatattc ttccagattg atcgaacaaa
      121 tggcggtttg ttttgtcaga tctaattgct tttcatggta aattttaatt ccttggatct
      181 tctgaatttt tttataaaat atttcaataa gattcatttc ctgttcacgt atcttatcaa
      241 ttccatatgt attgataaaa tcaatcgctg ccccaagtcc tacgattccg atcccattta
      301 atgttcctgc ttccaatgcc tgtggcagca ctcctggctg tgttttcgaa aatgataaga
      361 ttcctgttcc acctgttttt aacggacgga tctctacacc ttttctaata cagatccccc
      421 cgataccctg cggtcccatc aagcctttat gtccggtaaa acataatata tcgatattat
      481 cttccttcat ggagattggg atcactccag cactttgagc ggtatcaacg acaaataaaa
      541 ttcctttttc tctacaaagt tttccaacac tttggatatc aaacatttca cctgtcacat
      601 tggatgcatg cgtcatcacg atcatcttgg tatcttttgt gatcgcctgt ttgatatccc
      661 ccacatctgg tgaagtgatc gttaagcaaa ctccttgtcg ttccatctca tataatggac
      721 gaagaacgct gttgtgctcc ataaacgttg tgatcacatg atcaccatga tttaagcttc
      781 ctttgatcac tgtgtttaag ctttctgtaa ttccactggt aaatacaact tgttctgctc
      841 catagccgtc aaaaaactga tctactttcg tacgaacttc gtaaatcttt cttgcagcat
      901 ccagagaact ttcgttgaca cctcttccgc tgttgccaag tgccaatgcg ttcataactg
      961 catccttgac acactctggt ttcgtgatat gcgttgctgc ataatctgca taaattgttt
     1021 ctaccttttg tttctcatgg atacatatag gaacattcgg ttcttcctgc tgatcagaaa
     1081 gattattttt caaatataat aatgcttcca aaacaccacc tgcaatgcat ctggctttat
     1141 ctgaaattgt aaagcagtta tcgtattctt cagctctcgg atcgatatct gcgatcttaa
     1201 atccttttgt caccggatat ccatcacgaa ttaaacctct cagtaatcca tccatacttg
     1261 caggtacatc aacaatcgta ccttctggtg tttctatctt tgctaataac tggcctttgc
     1321 ttaccatatc tgtgatatga cagatatttc ttaagatccc tttagctggg gaatggatca
     1381 ctcgttcctt tccaaagcct ttgatcactc ctggaattcc tgtattcggg atcgcgctgc
     1441 cttctttgat gatccttcca agcctgtgtc cacgcatcgt ctcaatcaca acatcgacat
     1501 cttcccctgc tgtaaatcca ggtcctaaag cgatcgtgat cggtgccata tctcttgttg
     1561 taccgagatt tttcttcgca agaatggcat caacaactgc gatcggatgc aactgtttga
     1621 tcatttctcc atttggatcg atcattaatg ccggattccc tgctttcatg atctgttctg
     1681 cttctttgat atcatgtgca agatggcagg tcatatcctc aacctgccac ttttcttcat
     1741 aaactgcttc tgaaaatgca acatttctac gaattgcaga tggatgtgca atttctaaga
     1801 tcaatacatg aaaaccactt tttaccaatt tataaattgt acctgtggcg atatctccac
     1861 cgcctcttac aatgattaaa tctttcatag agattcctca aggtttgatc acttttcctg
     1921 ctttttccat catttctacg atatcataca tatttgtgat ggaaccaacc gctaatttat
     1981 ctgtaatatc atagaaattt aagcatgttc cgcaagtata tatttcgact ccttctgctt
     2041 ctaatgtttt taaatcttct aaagaatcag aatcttcgca tgtaagatat gctcctgaat
     2101 tataaaataa tacctttgat ggaagtttat cctgttttgt tactgcaaat aaaaacgctt
     2161 tgatcaatgt ctgaccaaga gctttctctc cttgtcccat ctcattggat gatacagcga
     2221 tgataaaatc atcctttgca gatggtgcac agacaatctc ttcttcctca tctgctggtg
     2281 tataattctc tgccatgatc tcaatcttaa attcattgtc tgagatcttc tcgcttttaa
     2341 cttcgcagtt tttaccttct gcaaatcttg taacattctg gacagctggt tcattatcta
     2401 ccagaacgat taattttgtc tctccatcca ttgattctaa tgctttcttt gtgtgtacaa
     2461 ccggaatcgg acaagcattc cctctttcat caatctgttt catacttaac ctcccattta
     2521 aatttctacc tgaaataccc cttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnntctaaag gggtcatcca tgccattcca catcctgctg tgatctcttt tggtactggg
     2641 atcaatcttc ctgcgatctg atgttttttg caggcacttt ccataccgat cgcctgtgtt
     2701 gttgtctcaa atgtgatcac tgtttttaat tctttttttc tcatgatatt ttatgattct
     2761 cctgaatttc caaagttttt tataaaagaa cgttttactg cttcagcaac ttctttctga
     2821 aagttttcat aacgattcag aatatcttcg ccttctggtg taaggtgnnn nnnnnnntcc
     2881 cccttgcttt ccaccggatt ttcgttctac taatttgact ccaaggtctg cttccgcacg
     2941 atttaagatc ttccatgctt tgcttgcagc cattcccatg gatttatatg cagcagataa
     3001 agaacctttc tctcttgtta atgataataa ctgtgcaact ccttttccaa aatctggttc
     3061 ttccataaaa actctgaccc tgaaatcaaa acgttttggt aaatatgatt tcatgtctac
     3121 cacccttttc cgaatccaca gtttggataa gtacaaactt tacatccaag acataatccg
     3181 ccttctccaa ggtgtgcaag ttcttctgca cttactttat catctgccat gatccttggc
     3241 agaataagat caaaaacagt tctctttgaa tacataacac atcctggaag tcccatgatc
     3301 ggaattatct gatctccttt tttataatat gcaagtaaaa acattgctcc tggcaaaaca
     3361 ggtgctccat aagatatgat atcggctcct gtattcttga tcgcaagtgg tgtcttgtca
     3421 tctggatcaa cactcattcc tcctgtgcat aataccattt ctgccccatt ttcgatagct
     3481 tctaagattg tttttgttaa aagttcattg tcatctggca actgttttgt actggtcact
     3541 tctgcaccgt attctttgat cttttctgcc acaactggag taaatgtatc ctcaattcta
     3601 tgataataca cttcatttcc tgtagctaag atcacggctt ttttcttctt ataaggcaga
     3661 agagacagga ttggtgtatc cccaaccctg tctttgactt cctgcatctt atctttttca
     3721 atgaccagtg gaatgattct cgtacctgct agtttgtccc ctgctttgac tggagtattt
     3781 ccagatcttg tcgcgatcat catctgtccg aaagaattga cggtaaatag tttttctttg
     3841 tcgatcttta agagtccgtc atattcggca gataattcaa tcttaccttc cttaacttct
     3901 gactctttca ttccttcatt cttgcacaga ttacaaagga tccttgctgc atcattttca
     3961 tgaagcatat cgtctccggc ttcccagacg tataaatgat cttttccaag ggataacaac
     4021 actgggatat cttcttcttt gactacatga cctttcctga atcttgcgtc ttttgtaaca
     4081 cctttgatga tctgtgtgat atcatgacat agcacagttc ctacagcatc ttttgtatcc
     4141 actaatttca ttgtttttca ccttctactt cttatttatt aattgtaaat ccataagatc
     4201 tgaagacttt t
//
LOCUS       DAOMUE010000177         4190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_5962_length_4190_cov_2.474728, whole genome shotgun sequence.
ACCESSION   DAOMUE010000177 DAOMUE010000000
VERSION     DAOMUE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5962_length_4190_cov_2.474728"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1153)
                     /locus_tag="PLC71_07945"
     CDS             complement(20..1153)
                     /locus_tag="PLC71_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM02609.1"
                     /translation="MSYYKKVTLKDIANDSGVSVSSVSMILNKREGVSFSNKTVEKVL
                     SSAKKLGYEISSSPKKNGTPAYNTQRSAKYIAIFCPNISNSYYSTIAQSIEQAAYQKG
                     FKTLIITTFRDEALEKEFLQDMIKLHISGIVFTMMPQCPQFLEKVAKKYPVIVIGDKT
                     TDIDLNVIETSNYTAGVLMAEHLYELGHRNIAFLTTTIGKSLSLAMRYQRLKAIQNTY
                     KKLCMNEPYNIVVKEAKIDPELERKNIFLEHGVGYQLCNECLDDRNLANPTAFIGNND
                     MVSYGIMDAILKKGYQIPDDFSVCGFDNDFASSLLPISLTTVEHYMEDKGKKAFDMIY
                     EKIQEKDDFFVQDDKCVIRIEYKSKLISRDSTNVARTRKNINL"
     gene            1424..2299
                     /locus_tag="PLC71_07950"
     CDS             1424..2299
                     /locus_tag="PLC71_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM02610.1"
                     /translation="MKVIQISDFHLRGDGKLSFQKADTMKALHQTIDYFCSMKDYELP
                     EFFVVTGDLSEGGTREGYEFMREGLRKLPRPVYIVPGNHDKRDFFLEMFTEETPVKED
                     IKPYICYTIDEYPVRVIVIDTSKPSCHSGGLSDRVAEWLEERITEYPEKPTLVFTHHP
                     PFVTGLPAMDEGFDQADRLAQILNKHENVTLCCGHMHTAIFTSWHKIPCVTCPPIAMQ
                     MEVDFRGKNDINLTEEEKKEEFKGGGDRFFLGNPGYLIHDLQGDRINTHYQTIPTGAD
                     YSGPWPFKYYEGEED"
     gene            2320..3627
                     /locus_tag="PLC71_07955"
     CDS             2320..3627
                     /locus_tag="PLC71_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM02611.1"
                     /translation="MEKNNFRKIATITLFESAVQWYCFLLYGTAAGTVFNKVFFSQTG
                     NGTTALILSYMSFAIGYIAGPFGAIFFGHIGDRKGRKVTMYASLLMMGISTSIIGILP
                     PAASAGVGVVIVLQLMRLAQCFGRGGTWGGGILMAYENVPENKRSFYAAIPQIGLPIG
                     FGLSSILIAVPTLLLPEDIFFTWGWRIPFLAAIILTLIVIRQKGEMMETEDYKKAQAK
                     LEAEEAEGKKHKVGFIPMVKGYWKTLLLGCGTRWVDGTFYNIFIVWILSYCINWLGMP
                     LIQTYIITIIACIFKIPFTLFGGWCAQKLGNTRTFVIGALASAVLSIPTLKVIELSKG
                     NLAITIIGIVLGWSIAYNLIWAVISSLWSSYFETEVRYSGISFVYHVPSFLVAGMVPT
                     ICTLLINYGKGDTIYVGIYSTVVALISAACALALKARHDRGEK"
     gene            3689..4190
                     /locus_tag="PLC71_07960"
                     /pseudo
     CDS             3689..4190
                     /locus_tag="PLC71_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024740.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
BASE COUNT         1401 a          644 c          847 g         1298 t
ORIGIN      
        1 aaaattttaa taacaaaagt tataaattga tattttttcg tgtacgagca acattcgtag
       61 aatcacgaga aataagtttt gatttgtatt caatacggat cacacattta tcatcttgaa
      121 cgaagaagtc atccttttcc tgaatctttt catagatcat atcaaatgct ttctttcctt
      181 tgtcttccat gtaatgctcc acagtcgtca atgagattgg aagcagactg gaagcaaagt
      241 cattgtcaaa cccacataca ctgaaatcat ctggaatctg atagcctttt ttaaggatcg
      301 catccatgat cccataagaa accatatcgt tgttaccgat gaatgcagta ggatttgcga
      361 gatttctgtc atcaagacat tcattgcata attgataacc aacgccgtgt tctaagaaaa
      421 tatttttacg ttcaagttct ggatcaattt tggcttcttt tacaacgata ttataaggtt
      481 cattcataca aagtttctta taggtgttct gaattgcttt taaacgttga taacgcatgg
      541 caagagacag gcttttacca atcgtggttg ttaaaaatgc gatattacgg tgtccaagtt
      601 catataaatg ttcagccatc aaaactccag cagtatagtt acttgtctcg atgacattca
      661 gatcaatatc tgttgtttta tctccgataa cgatcacggg atatttctta gcaacctttt
      721 ctaaaaactg tgggcattgg ggcatcatag taaaaacaat accactgata tgcagtttga
      781 tcatatcctg aaggaactct ttttccaagg cttcatcacg gaaagtcgtg atgatcaaag
      841 ttttaaatcc tttttgataa gcagcctgtt caatggattg tgcgatcgta gagtaataag
      901 agtttgatat gttagggcaa aaaatagcaa tatactttgc ggatctttga gtattgtaag
      961 caggtgtacc attctttttt ggggaagaag aaatctcata tcctaatttt ttcgcagagg
     1021 atagaacctt ttcaaccgtt ttattggaaa aagaaactcc ttcgcgttta tttaagatca
     1081 tggaaacaga acttacggaa acacctgaat catttgcaat gtcttttaat gtaacttttt
     1141 tataatatga cataaatccc tcctgaataa cattaaaagt ttaataaaaa acagaaagaa
     1201 actattcaaa atagacaaaa attgatgaga attattagtc aataatctgt atttttatta
     1261 aaacaattaa aaataattga ataaaacaat taaaaaagtt aaaattcaat taaagaaaca
     1321 gagatacaaa aattaaaagt ttaattaatt ttaacacatt tattattccg gaacaataaa
     1381 aatgtattct cgagagcgtg atatgaaaaa ggaggttgca tttatgaaag ttattcagat
     1441 ttcagatttc catttaagag gtgatgggaa attatctttc cagaaagcag atacaatgaa
     1501 agccttgcat cagacgatcg attatttttg ttcaatgaag gattatgaat taccagaatt
     1561 ttttgtagtg actggggatt tgtctgaggg tggaacaagg gaaggatatg agtttatgag
     1621 agagggactt agaaaacttc caagaccagt ctacattgtt cctgggaacc atgataaaag
     1681 agacttcttt ttagaaatgt ttacagagga aactccggta aaagaggata tcaaacctta
     1741 tatctgctat acaatcgatg aatatccagt tcgagtgatc gtaattgata caagcaaacc
     1801 atcttgtcat tcgggtggat taagcgatcg ggtagcagag tggctggaag aaagaattac
     1861 agaatatcct gagaaaccaa cattagtatt tacacatcat ccaccatttg taacaggatt
     1921 gccagcaatg gatgaaggat ttgatcaggc agacagactt gcacagatct taaataaaca
     1981 tgaaaatgtt acgttatgtt gtggtcatat gcatactgct atttttacat catggcataa
     2041 aattccatgt gtgacatgtc caccgatcgc aatgcagatg gaagttgatt ttagggggaa
     2101 aaatgatata aatcttacag aggaagagaa aaaagaggaa tttaaaggtg gaggagatcg
     2161 cttcttcctt ggaaatccag gatatttgat tcatgatctt caaggagatc gcattaatac
     2221 acattatcag acaattccaa cgggtgctga ttattcagga ccatggccat ttaaatacta
     2281 tgaaggggaa gaagattaac aagaaggagg ataaaatata tggaaaaaaa taattttcga
     2341 aaaattgcaa cgatcacatt gtttgaaagt gcggtgcaat ggtattgttt cttattatat
     2401 ggaacagcag caggaactgt gtttaataaa gtgttttttt cacagacggg aaatggaaca
     2461 acagcgctga tcctttctta tatgtcattt gcgattggat atattgcagg tccatttggt
     2521 gcgattttct ttggacatat tggtgataga aaaggtcgaa aagttacgat gtatgcatct
     2581 ttattgatga tgggaatttc gacatcaatc attggaatcc tgccaccagc agcaagcgca
     2641 ggagttggag tagttatcgt tcttcagtta atgcgtctgg cacaatgctt tggccgtgga
     2701 ggaacatggg gtggaggaat cttaatggca tacgaaaatg tgccagagaa taagagatct
     2761 ttctatgcag cgattccaca aatcggtctt ccgattggat tcggattatc ttctattttg
     2821 atcgcagtac caacattatt attaccagaa gatattttct ttacatgggg atggagaatt
     2881 ccttttttag cagcaattat cttgacattg atcgttatcc gtcaaaaagg tgaaatgatg
     2941 gaaacagaag attataagaa ggcacaggca aaattagagg cagaagaagc agaaggaaag
     3001 aaacataaag tcggatttat tccaatggta aaaggatact ggaaaacatt acttcttgga
     3061 tgcggtacaa gatgggtgga tggaacattt tataatattt ttatcgtatg gatcttgtcc
     3121 tattgcatta actggcttgg aatgccactg atccagactt atattattac gatcatagca
     3181 tgtattttta agattccatt tacactgttt ggtggatggt gcgcacaaaa attgggaaat
     3241 acaagaacat ttgtaattgg agctttagca tcagctgttt tgagtattcc tacactgaaa
     3301 gttattgaat taagcaaagg aaaccttgcg atcacaatta ttggaatcgt tcttggctgg
     3361 agtattgcgt ataacctgat ctgggctgtt atcagttctt tatggtcttc ttactttgaa
     3421 acagaagtac gttattcagg aatttctttt gtatatcatg tgccaagttt cttagtagct
     3481 gggatggtac caacaatctg tacattgctg attaactatg gaaaaggtga tacgatttat
     3541 gtaggtatct attctacagt tgtagcgctg atcagtgctg catgtgcact ggctctaaaa
     3601 gctcgacatg atcgaggaga gaaataaaga atgaagggat ttgcagggga atcatatgtt
     3661 tgaaaaaatc aaatgtttta tccttgatat ggatggaacg atttatcttg gaaatgaact
     3721 attcccattt acgaaagact ttttaaagaa agttgaggag accggaaggg aatattactt
     3781 ctttaccaat aattcatcaa aaagccagcg gcttatattg agaaacttgg gaagcttggg
     3841 ataccgatca agaaagatca gatgatgatc tcaagtcatg tgatcatcaa atatcttaaa
     3901 gaacattatg atggaaagag catttatgta gtagggacaa catctctgat ccaggaattt
     3961 caatattttg atatgaatct gacagaagaa gatccagata tcgtagtcct gggatttgat
     4021 accactttaa cttatgaaaa gctttccaaa gcatgtcatt atattcggaa tggctgtata
     4081 tattttggaa tcaatccaga ctggaactgc ccaatggaag gcggagcatt tattccagac
     4141 tgtggatcaa tagcaaaact tatagaggct tcaacgggaa gatttccaga
//
LOCUS       DAOMUE010000178         4113 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6094_length_4113_cov_2.327748, whole genome shotgun sequence.
ACCESSION   DAOMUE010000178 DAOMUE010000000
VERSION     DAOMUE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6094_length_4113_cov_2.327748"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="PLC71_07965"
     CDS             complement(<1..1385)
                     /locus_tag="PLC71_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HRM02612.1"
                     /translation="MMNNEMTKSNEQNILVGIDVGSTTTKIVAVDANDRHQLLFSDYA
                     RHHANQIASVIRSIKKFCGHIRDKKIRLCLTGSGAKPVASILKVPFVQEVAANAIALQ
                     DQFDQVGTAIELGGQDAKMIFFKDSENGQSQSVSDMRMNGSCAGGTGAFIDEIASVLK
                     VPVEQFNELAQKGTHLYDISGRCGVYAKTDIQPLLNQGAAKEDLALSAFHAIAKQTIG
                     GLAQGLEIKKPVAFEGGPLTFHPTLVKVFAERLDLKEDEILCPKHSELMIAYGAALSL
                     DKMFADSKPKDVNKMLVILEDAQKDHSHVSGEIAKPFFESKEEENAFKEAHKKKQVTW
                     KKPQKGEVVRCFLGIDAGSTTTKFVLLDEQEEILDSFYAPNEGDPLKVAKEALICLRK
                     RYEEAGAELEILSAGTTGYGEMLFSKAFEIPCHTVETVAHARASEKYVKDASFILDIG
                     GQDMKAIWLEDG"
     gene            complement(1482..2018)
                     /locus_tag="PLC71_07970"
     CDS             complement(1482..2018)
                     /locus_tag="PLC71_07970"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRM02613.1"
                     /translation="MIKQIGTLNIEIERRKIKNINIYVKPPNGDVLVTVPMRVSNEEV
                     FRFLKKKEEWILKNHEKVKNRQNSSQQEINLEQRKWLEDKIIEYAMRWESIMKVHANG
                     FTIRDMKTRWGSCSIHSKKIRMNLQLAVKSEECVEYVLVHELCHLLEPSHNQRFYDLM
                     SHFLPDWRERKQKLNEKV"
     gene            complement(2024..3598)
                     /locus_tag="PLC71_07975"
     CDS             complement(2024..3598)
                     /locus_tag="PLC71_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HRM02614.1"
                     /translation="MKQETGRLKEILHVLNQHNVTHGINPEKLCSILEDLGPTYVKLG
                     QIMSMRSDILPEKYCHELARLRTDVKPMSFNEIWKILNKELHNDPGQLFQKINQIPVG
                     CASIAQVHEAVLGDGSKVVLKIQRPQIKQIMAEDIALLKKASGFLNFATGTGELIDFR
                     KVIDELWETSKEEMDFEKEAQHLERFYENQKDVAYVTCPKVYKEYSNEHLLVMSYVDG
                     IQIDHIEELDRNGYDRKEIAQKTAQNYCKQILADGFFHADPHPGNLWISGGQIVWLDL
                     GMAGDLSEHYRAIMKRAITAILKNDIYELKNAFLSFGKPTEKIDHASLYTDLDDMVGK
                     YMNMDFGTMDLGELMEDAIGLLKKHKIAIEPDITLLARSMVTMEGTLKLCSPEVNMIQ
                     ILTSYMSANMFHEIDIKKEIRHALRDIYGSSKKSLEIPAQVSDLLNITKNGQVRVNIE
                     SAQIEKAGRELKKGFDHLLLVLIVMALWFSSAILCLSDVTPRVLGMPYLSVAGFISGF
                     LIVLYVVIHMLIQRKK"
     gene            complement(3608..4006)
                     /locus_tag="PLC71_07980"
     CDS             complement(3608..4006)
                     /locus_tag="PLC71_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02615.1"
                     /translation="MDFTNGMKKAFLASVGAAATTAEVTKDILEKMIEKGERTVEHGK
                     VLNQELKHNAKQKVKEHVSIQVTKKYDKAFECVDEMSPEDLALLKERIAKAEALQKEK
                     QEAEEDVEVEESEATTDSETSQVQEDETEQ"
BASE COUNT         1102 a          960 c          573 g         1478 t
ORIGIN      
        1 ccatcctcta accagattgc tttcatatcc tgtccaccaa tatccaaaat aaaagaggcg
       61 tctttgacat atttttcaga agctcttgca tgtgctaccg tctcaaccgt atggcaaggg
      121 atttcaaaag ctttggaaaa cagcatttct ccatacccag tcgtaccagc tgataaaatt
      181 tctaattctg ctcctgcttc ttcatatcgt tttcttaaac agatcaaggc ctctttcgct
      241 actttcagcg gatccccttc attcggtgca taaaaagaat ccagaatttc ttcttgttca
      301 tctaataata caaattttgt tgtggtactt cctgcatcaa tcccaagaaa acatcgaaca
      361 acctctcctt tttgtggttt cttccatgtc acctgtttct tcttatgtgc ttctttaaaa
      421 gcattttctt cttcttttga ttcaaaaaat ggttttgcaa tttctcctga aacgtgggaa
      481 tgatctttct gtgcgtcttc cagtattact aacattttat tcacatcttt tggtttgctg
      541 tctgcaaaca tcttatccaa agataatgct gccccatatg caatcattaa ttctgaatgc
      601 tttggacaaa gaatctcatc ttctttcaaa tccagacgtt cagcaaaaac ttttaccaga
      661 gttggatgaa atgttaatgg gccaccttca aatgcaacag gttttttaat ctccaatccc
      721 tgtgccaacc ctccgatcgt ctgtttcgcg atcgcatgga atgcggataa cgcaaggtct
      781 tccttggctg ctccctgatt cagaagtggt tggatatctg tctttgcata taccccgcat
      841 cttccagaaa tatcatataa atgtgttcct ttttgtgcca actcattaaa ctgttccact
      901 ggtactttta atacggatgc aatttcatca ataaatgctc ccgttccacc cgcacagctt
      961 ccattcatac gcatatcgga aacactttgt gactgtccat tttcagaatc tttaaaaaag
     1021 atcatcttgg catcctgtcc acctaactca attgccgttc cgacctggtc aaactgatct
     1081 tgtaatgcga tcgcattggc tgcaacttcc tgcacaaatg gtacttttaa aatagatgcc
     1141 actggttttg ccccagaacc agtcagacac aatctgatct tcttatctct tatatgtccg
     1201 caaaacttct taatgcttcg gatcacactt gcgatctgat ttgcatgatg tcttgcataa
     1261 tcagaaaata acagttgatg acggtcattg gcatcaactg ctactatttt ggttgtcgta
     1321 gatcctacat cgatcccgac taatatattt tgttcgtttg attttgtcat ttcgttattc
     1381 atcattcgtt atcgtctttt ctccttcttt tgagctttac gttttatcat aactcaaatt
     1441 aatagaaaat gtaagataaa aaatggattt taacactttc ttcacacttt ttcatttagt
     1501 ttctgcttac gttccctcca atctggaaga aaatgactca tcaaatcata aaatctctgg
     1561 ttatggcttg gttctaaaag atgacataat tcatgcacca aaacatactc tacacattcc
     1621 tctgatttta cagccagctg cagattcatc cgaatctttt tactgtgaat actacagctt
     1681 ccccatcttg ttttcatatc tcgaatcgta aaaccattcg catgaacttt catgatcgat
     1741 tcccatctca ttgcatattc tatgatcttg tcttccagcc atttcctttg ttccagatta
     1801 atttcctgct gtgatgaatt ttgtctgttt tttacttttt catggttttt taaaatccac
     1861 tcttcttttt tcttaaggaa acgaaaaacc tcttcgttgg aaactctcat tggaactgtt
     1921 accaaaacat ctccgtttgg aggtttcacg tagatattta tatttttaat cttccttcgc
     1981 tcaatctcaa tatttaatgt tcctatttgt tttatcatta ttcttatttc tttctctgaa
     2041 tcaacatatg gatcactaca tacaaaacaa tcagaaaccc tgatataaat cctgccacac
     2101 taagatatgg cattcctaac actcttggtg ttacatcact tagacataaa attgcagagc
     2161 taaaccaaag tgccatcacg atcaatacaa gcaacagatg atcaaatcct ttttttaatt
     2221 ctctacctgc tttctcgatc tgcgcagatt caatgtttac ccttacctgt ccattctttg
     2281 taatgttcaa aagatctgat acctgtgctg gtatctccaa agatttctta gatgacccat
     2341 aaatatctct caaagcatgt cttatctctt tctttatatc aatctcatga aacatattcg
     2401 cagacatata agaagtcaat atctgtatca tattcacttc tggagaacat aatttcaatg
     2461 ttccttccat agtcaccata cttcttgcaa gcagcgtaat atctggttcg atcgcaatct
     2521 tatgtttctt caaaagccct attgcatctt ccatcaattc tccaagatcc atggtcccga
     2581 aatccatatt catgtatttt ccaaccatat catcaagatc cgtatacaaa ctagcatggt
     2641 cgatcttctc tgtcggtttt ccaaacgata agaatgcatt cttaagttca taaatatcat
     2701 ttttaagaat agctgtgatc gcacgtttca tgatcgcacg gtaatgttca gatagatcac
     2761 ctgccattcc aagatcaagc catacgatct gaccaccact aatccataga ttccctggat
     2821 gtggatctgc atgaaaaaaa ccatccgcca aaatctgttt acaatagttt tgtgctgttt
     2881 tctgggcaat ctccttccta tcatacccat tgcgatcaag ttcctcgata tggtcgatct
     2941 ggattccatc aacataactc atcaccagca aatgctcatt cgaatattcc ttatatactt
     3001 tcggacatgt cacatatgca acgtccttct ggttctcata aaatcgttcc aaatgttgtg
     3061 cttctttttc aaaatccatt tcttctttgg atgtctccca caactcatca ataacttttc
     3121 taaaatcaat caattcaccg gttcctgttg caaaatttaa aaatccagaa gccttcttta
     3181 acagagcaat atcttctgcc atgatctgtt taatctgcgg tctctggatt tttaatacaa
     3241 ctttactgcc atctcctaaa acggcctcat ggacctgtgc gattgatgca cacccaactg
     3301 gtatctggtt aatcttctga aatagttgac cggggtcatt atgaagttct ttattcaaga
     3361 tcttccatat ctcattaaaa ctcatcggtt ttacatccgt gcgaagtctc gctaactcat
     3421 gacaatactt ctctggcaat atatccgaac gcattgacat gatctgccca agctttacat
     3481 aagttggacc aagatcttct aaaatactgc ataacttctc tggattgatc ccatgtgtga
     3541 cattatgctg atttaataca tgcaagatct ctttcagtct tcctgtttcc tgtttcataa
     3601 gtacctctta ttgttcagtt tcatcctctt gtacttgtga tgtctctgaa tctgttgttg
     3661 cttcactttc ttccacttca acatcttctt cagcttcctg tttttctttc tgaagtgcct
     3721 cagcttttgc aattctttct tttaaaagtg caagatcttc tggggacatt tcatcaacac
     3781 attcaaatgc tttatcatat ttcttagtca cttggataga tacatgttct ttgactttct
     3841 gttttgcatt gtgctttaat tcctgattta acacttttcc atgttctaca gtacgttcac
     3901 ccttttcaat cattttctca agaatgtctt tggtaacttc tgcagttgtt gctgctgcac
     3961 ctacacttgc aagaaatgct tttttcattc catttgtgaa atccatatgt catcatcctt
     4021 tctataattt agcatgatca tttgtcactt ttattttacc actatagatc ttcaaggaca
     4081 accgtaaatg gtccatcatt tattaaagca act
//
LOCUS       DAOMUE010000179         4086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6159_length_4086_cov_2.911684, whole genome shotgun sequence.
ACCESSION   DAOMUE010000179 DAOMUE010000000
VERSION     DAOMUE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6159_length_4086_cov_2.911684"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..941)
                     /locus_tag="PLC71_07985"
     CDS             complement(66..941)
                     /locus_tag="PLC71_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM02616.1"
                     /translation="MEFRQIQTFMQIAQVKNFSKTAELLGYSQSAVTVQIRQLETELG
                     VRLFDRTGKKVILTPKGKEFLIHANKIIDEMHKAKDAMNDTAELKNPLHIGTIESLCT
                     VKLQEIVQRFREEHPQVRIQITIGSPERLIQKMEHNELDVIYILDTPRWNKNWVKVME
                     KAEPVIFVSAPNYKLAGKKNIEVEEILNAPFYLTERNANYRQALEQELALHKQTLSPV
                     LECSDTAFIKKMLKTGKGLSYLPLFVVEKDIEEGKIAKLDVKDIDITMYRQVFYHANK
                     YMTKEMEKFVEYCRL"
     gene            1114..2337
                     /locus_tag="PLC71_07990"
     CDS             1114..2337
                     /locus_tag="PLC71_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRM02617.1"
                     /translation="MEKKKFKLGLVPKLIIAIIIGILFGQFLPTGFCRFVVTLSGFFS
                     TYLKFIIPLMILAYVTMGIADLSQGAGQLLLITVALAYGSTLLAGTASYLVSSNLFPH
                     FMTSGALDQIAATADASLKPYFSLTITPLFDTLSAVVLAFILGLCLSTMKGKEIGNSL
                     YNGMSDFSTIIDKVLHKTIIPLLPLYICGTFTDMTKSGKTFAILGILWKVFLVVIIMH
                     FVCITIQFIIAGSVSKKNPFSLIKNQVPGYTTALGTQSSAATIPVNLECAKNDGVSAQ
                     IRNFVVPLCANIHMAGSMITITACATAVCLMNQLPISLATVIPFIMTLGVAMVASPGA
                     PGGSIMTALPFLYMVFGTTAGDTNGPICALMVALYITQDSFGTACNISGDNAIGIVVD
                     SIYKKFILKEDTVEA"
     gene            2341..3009
                     /gene="sdaAB"
                     /locus_tag="PLC71_07995"
     CDS             2341..3009
                     /gene="sdaAB"
                     /locus_tag="PLC71_07995"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HRM02618.1"
                     /translation="MSFISAFDVMGPNMIGPSSSHTAGAARIAFLARKMLNSTLKKVQ
                     FTLYGSFAKTYHGHGTDRALLGGIMGFGTDDMRIRDSFEIAKDIGLDFSFVPNEIETD
                     VHPNTVDILMANENGDHIMIRGESLGGGKARICRINDVEVDFTGEYSTLIVIQKDKPG
                     VVTYITKCLSDQGVNIAFMRLFRESKGNTAYSIVESDGLLPENIAEEIRKSPNVSDVM
                     IIQL"
     gene            3028..>3282
                     /locus_tag="PLC71_08000"
     CDS             3028..>3282
                     /locus_tag="PLC71_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRM02619.1"
                     /translation="MDFKNANELLDLCKKHDLPISEVMRQREIVEGEKEADTVHDAMA
                     RVLEIMKNAAFTPIKEPVRSMGGLIGGEAKKLYLHQTSGKS"
     assembly_gap    3283..3292
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3293..3949
                     /gene="sdaAA"
                     /locus_tag="PLC71_08005"
     CDS             <3293..3949
                     /gene="sdaAA"
                     /locus_tag="PLC71_08005"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRM02620.1"
                     /translation="EAKKIASRYHSGIGICGDLLEKSMIYAMATLETNASMGLIVASP
                     TAGSAGIVPGLLLGLQEVHKLDDEQIIQALFNASAIGYLAMRNATVAGAVGGCQAEVG
                     VASAMAASAIVELLGGDPEQCLGAASTVLMNMLGLVCDPVGGLVEYPCQNRNAAGVAN
                     AIIAAEISLSGIHQLIPFDEMLNAMYIVGKRLPAELRETAQGGCAATPSACAACGGCA
                     "
BASE COUNT         1124 a          798 c          813 g         1341 t
ORIGIN      
        1 actgtcaata ggtgagtgct aattttttta aaattttatt tctaacatgt tatcaaacca
       61 acaaatcaca acctgcagta ctccacaaat ttctccattt cttttgtcat atatttattt
      121 gcatgataaa acacttgtcg atacattgta atatcaatat ctttcacatc taattttgcg
      181 atctttcctt cttcaatatc cttttctaca acaaataatg gaagataaga aagccctttt
      241 cctgtcttta gcatcttctt gataaacgcg gtatcactgc attcaagtac tggcgataat
      301 gtctgtttat gcagagctaa ttcctgttct aatgcctgtc tgtagtttgc atttcgttct
      361 gttagataaa acggtgcatt taagatttcc tcaacctcta tattcttttt ccctgctaac
      421 ttataatttg gtgcagacac aaagatcaca ggctccgctt tttccatgac tttcacccag
      481 tttttattcc atcttggtgt gtccagaata taaatgacat cgagttcatt gtgttccatc
      541 ttctggatca ggcgttctgg agaaccaatc gtgatctgaa ttctaacctg tggatgttct
      601 tcccgaaacc gctgtacgat ttcctgcaat ttgacagtgc agagtgattc aatcgttccg
      661 atatgtaatg gattttttaa ctctgcagta tcattcattg catcttttgc cttgtgcatt
      721 tcatcaatga tcttatttgc atgtattaaa aattcttttc ctttcggtgt gagtatcact
      781 ttttttcctg tacggtcaaa taaccgcact cccaattctg tttctaactg acggatctgt
      841 actgtcacgg cagattgcga ataacctagt aattctgcgg tttttgagaa attcttcact
      901 tgtgctatct gcataaatgt ctgtatctga cggaattcca ttttttctcc tttgtactat
      961 tatgaataat tttcaatgtt atcattaaat ctatgaattt gattcatcta tacgtggatg
     1021 atacaataca aacataaaga cgtcaaacat tttttgtgca cattaaacaa actacgatga
     1081 tatgtgatta tgcttttaga ggagtaatta aaaatggaaa agaaaaaatt taaacttggt
     1141 ctggtaccaa aactcattat tgccatcatt attggtatcc tgtttggaca gttccttcca
     1201 actggattct gccgctttgt tgtaacactg tctggattct tcagtaccta cttaaaattc
     1261 atcattccac tgatgatcct tgcttatgta actatgggaa tcgctgatct gtcacagggt
     1321 gctggacagc tgcttttaat taccgttgct cttgcttatg gttcaacttt acttgctggt
     1381 acagcttctt accttgtatc atccaatctg ttcccacact ttatgacttc tggggcatta
     1441 gatcagattg ctgcgactgc tgatgcgtca ctgaaacctt atttttcact gacgatcaca
     1501 ccgctgtttg atacattatc tgctgttgtt cttgctttca ttttaggatt atgcttatcc
     1561 acaatgaaag gtaaagagat tggaaacagc ctttacaacg gaatgagcga tttctcaacg
     1621 atcatcgata aagtccttca caaaacgatc attcctttat tgccattata tatctgcggt
     1681 acatttactg atatgacgaa atcaggaaaa acatttgcaa tccttggaat cttatggaaa
     1741 gtattccttg tagtgatcat catgcacttt gtatgtatca cgatccagtt tataattgca
     1801 ggttctgtca gcaaaaagaa tcctttctct cttattaaaa atcaggttcc tggttatact
     1861 acagctttag gaactcagtc ttctgctgct acaattcctg tcaatctgga atgtgcaaag
     1921 aatgacggtg tcagtgctca gatccgtaac tttgtagtgc ctctttgtgc caatatccac
     1981 atggcaggtt ctatgatcac gatcacagca tgtgcaactg ctgtatgttt gatgaatcag
     2041 ttaccgatca gccttgcaac tgttattcca tttattatga cacttggtgt tgcaatggtc
     2101 gcttctcctg gagctccagg tggatctatc atgactgcat tgcctttcct ttacatggta
     2161 tttggtacaa cagcaggaga tacaaacgga ccaatctgtg ctttaatggt tgcactttat
     2221 atcacacagg attctttcgg aactgcatgt aacatttccg gagataatgc gatcggaatc
     2281 gttgtagatt ctatttacaa gaaatttatt ttaaaagaag atacagtgga ggcttaattt
     2341 atgtctttta tcagtgcatt tgatgttatg gggccaaata tgattggtcc ctcaagctcc
     2401 cacacagccg gagctgcaag gatcgcattt ctcgcaagaa aaatgttaaa tagtacactg
     2461 aaaaaagttc agtttacact ctatggatct tttgcaaaaa cttatcatgg acacggaacg
     2521 gatcgtgcct tattaggtgg gatcatggga tttggaaccg atgatatgag aatccgtgat
     2581 tctttcgaga ttgcaaaaga cattggactt gatttctctt ttgtaccaaa tgaaatagaa
     2641 actgatgtcc atccaaatac agtggatatc ttaatggcaa atgaaaacgg agatcacatc
     2701 atgatccgtg gggaatcttt aggcggtgga aaagccagaa tttgccgtat caatgatgta
     2761 gaagttgatt ttaccgggga atacagtacc ttgatcgtga tccagaaaga taaacctggc
     2821 gttgttactt atatcacaaa atgtctgagt gatcagggtg ttaatatcgc ttttatgcgt
     2881 cttttcagag aatctaaagg aaatacagct tattctatcg ttgaatccga tgggcttctt
     2941 cctgaaaaca tcgcagagga gattaggaaa agcccaaatg tctcagatgt tatgatcatt
     3001 cagctgtaga aaggagactg aaatattatg gattttaaaa acgcaaatga attgcttgat
     3061 ttatgtaaaa aacatgatct tccaatctcc gaagtgatga gacagcgtga gatcgtagaa
     3121 ggggaaaaag aagctgatac ggttcatgat gcaatggcaa gagttcttga gatcatgaaa
     3181 aatgctgctt ttactccgat caaagaacca gttcgttcca tgggtggact gatcggtggc
     3241 gaagcaaaaa aactctatct tcatcagact tccggcaaaa gtnnnnnnnn nngaggcaaa
     3301 gaagattgct tcccggtatc attctggaat tggaatctgt ggtgatctgt tagaaaaaag
     3361 tatgatctat gcgatggcga ctcttgagac gaatgcatcc atggggttga tcgtagcatc
     3421 cccaaccgca gggtctgccg gaatcgttcc tggactttta cttgggcttc aggaagttca
     3481 taaattagat gatgaacaga tcattcaggc actgtttaat gccagtgcga tcggatatct
     3541 tgctatgaga aatgcaacgg ttgcaggtgc tgtcggcggg tgtcaggcgg aagtcggtgt
     3601 tgcttctgcg atggctgctt ctgccattgt cgaactgctt ggcggtgatc cagaacaatg
     3661 tcttggagct gcctctaccg ttttaatgaa tatgttaggt cttgtttgtg accccgttgg
     3721 cgggttggtg gaatatccat gccagaaccg taatgccgca ggtgttgcaa atgcgatcat
     3781 cgcagctgag atttctttat ctggaatcca tcagctcatc ccgtttgacg aaatgttaaa
     3841 cgcaatgtat atcgttggta aacgactgcc agctgaacta agagaaaccg cccagggagg
     3901 ctgcgctgca actccttctg catgtgctgc atgcggaggt tgtgcgtaaa aattccttca
     3961 gaacacattt ttaaatagta ggtaataaat caatctcttt tttgcctctt ttgggcgttt
     4021 tcatatattg ggatctaaaa gagtgtctgc atcacttaat gatgcagaca ctctttattt
     4081 actata
//
LOCUS       DAOMUE010000180         4061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6215_length_4061_cov_2.763105, whole genome shotgun sequence.
ACCESSION   DAOMUE010000180 DAOMUE010000000
VERSION     DAOMUE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6215_length_4061_cov_2.763105"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLC71_08010"
     CDS             complement(<1..409)
                     /locus_tag="PLC71_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRM02621.1"
                     /translation="MYTIGQVSTMFDLPVSTLRYYDKEGFFPNLERKGNIRYFSDNEL
                     EALRIIECLKKSGLEIKDIKQFFLWVSEGSASYEKRKELFEARKSAVETEIQELQKTL
                     SLLKFKCWYYETAMKDGNEDAINAMLPDKLPENI"
     gene            526..2274
                     /locus_tag="PLC71_08015"
     CDS             526..2274
                     /locus_tag="PLC71_08015"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRM02622.1"
                     /translation="MYIENINGPEDVKKLSEDQLNVLAEEIRDALLKKLSKHGGHFGP
                     NFGMVEATIAMHYVFESPKDKIVYDVSHQSYPHKMLTGRKDAFLYEEHYDDVSGYSNP
                     GESEHDHFTIGHTSTSISLALGLAKARDLKEENGNVIAVIGDGSLSGGEALEGLDYAA
                     ELGGNLIIVVNDNDMSIAENHGGLYENLKALRETNGESKCNLFKAMGLDYIYVKEGNK
                     VADLIEAFKQVKDTKKAVVVHINTQKGKGYKLAEEHKEDWHYCAPFDVETGKPLDSFD
                     GEDFSSVTAQYLLKKMKEDKKVVAITSATPTVMGFTEDKRKEAGKQFIDVGIAEETAM
                     AMAAGIAAGGGKPVYGVYSTFVQRTYDQITQDLCIDSNAATIVTFWGSVYGMNDVTHL
                     GLQDIPMMSNIPNLVYLAPTTKEEYLAMLDWSIDQDQYPVAIKLPGGEMISDRKKITK
                     DFSQLNKYEVTEKGSKVAVIGLGTFYNMGCEVAKAVEEKTGTKATVINPYYITGIDEV
                     LLEDLKKDHDVVITLEDGFLEGGFGEKIARFYGDSDVKVLNYGVKKELLDRYDVTELL
                     EKNHLTVQQIVGDLKF"
     gene            complement(2917..3990)
                     /locus_tag="PLC71_08020"
     CDS             complement(2917..3990)
                     /locus_tag="PLC71_08020"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRM02623.1"
                     /translation="MLRIGILTSGGDCQALNAAMRGVIKGLSVKRNDLEIYGFRDGYK
                     GLIYDIADRMTPDDFSGILNRGGTILGTSRQPFKKMRIPDENGLDKVEAMKNNYRKYK
                     LDCLVVLGGNGTHKTANMLREEGLNVVTLPKTIDNDLWGTEMTFGFQSAVDIATDTID
                     RIHTTASSHSRVFIIEVMGHKVGHVTLQSGIAGGADVILLPEIPYDIDKVAEVVENRF
                     AAGKKFSIIAVAEGAISKEDAKLKKKQYKAKLAERRYPSVAYEIAAALEEKTNREIRV
                     TVPGHTQRGGAPCAYDRVLATRVGAYAAELILNKEYGYMVGIVDEDTKKVPLSEVAGK
                     LKYVDPKCQMIKDAKTIGISFGE"
BASE COUNT         1348 a          724 c          780 g         1209 t
ORIGIN      
        1 gtatgttttc aggaagttta tcaggcaaca tggcatttat agcatcttca ttaccgtctt
       61 tcattgctgt ttcataatac cagcacttga attttaagag agaaagagtt ttttgaagtt
      121 cttggatttc agtttcaaca gctgattttc tggcttcaaa taattctttc cttttttcat
      181 agcttgcgct gccttcagat acccagagaa aaaattgctt tatatctttg atctcaagac
      241 cggatttctt cagacattca ataatacgaa gcgcttccaa ttcattatca ctaaaataac
      301 ggatatttcc tttacgttcc agattaggga aaaatccttc cttgtcataa taccttaagg
      361 tagaaacagg aagatcaaac attgtagaga cttgaccgat cgtgtacata gaaaaacctt
      421 ctttcaaaaa tattcttgac ctaaagttaa ctttaggttg tatgatataa atatcataca
      481 ctattgctag tgtatttaca agaaaccaga caaggagaaa aatagatgta tattgaaaat
      541 attaatggtc cagaagatgt caagaaatta tccgaggatc agttaaatgt acttgcagaa
      601 gaaattcgtg atgcattatt aaaaaaatta agtaagcatg gaggtcattt tggaccgaat
      661 ttcggtatgg tagaagcaac gatcgccatg cattatgtat ttgaatcgcc aaaagacaag
      721 atcgtatatg atgtttccca tcaatcttat ccacacaaaa tgttgactgg acgtaaggat
      781 gcattcctat atgaagaaca ttatgatgat gtatcaggat acagtaatcc aggagaaagt
      841 gagcatgatc atttcacaat tgggcataca tcaacttcta taagtcttgc acttggactt
      901 gccaaagcaa gagacctaaa agaagaaaat ggaaatgtga tcgcagtgat cggagatgga
      961 tctttaagtg gaggggaagc attagaaggt cttgattatg ccgcagaatt aggtggaaat
     1021 ctgatcattg tagttaatga taacgatatg tcgatcgcag aaaaccacgg tggattatat
     1081 gagaatctaa aagcgcttcg tgaaacaaac ggagaatcta aatgcaatct attcaaagcc
     1141 atgggattag attacattta tgtcaaagaa ggaaataaag tcgctgatct gatcgaagca
     1201 ttcaaacagg tcaaagatac aaaaaaagca gttgtcgttc atattaatac gcaaaaagga
     1261 aaaggatata aattagctga ggaacataaa gaagactggc attattgtgc gccatttgat
     1321 gtggaaacag gaaagccttt agattctttt gatggtgagg atttctcaag tgtaacagca
     1381 caatacctgt taaagaaaat gaaagaagac aagaaggttg tagcgatcac ttctgcaaca
     1441 ccaacagtta tgggatttac agaagataaa cgtaaggaag ctggaaaaca gtttatcgat
     1501 gttgggatcg cggaagaaac agcaatggca atggcggcag gaatcgcagc aggtggtgga
     1561 aaaccggttt atggagtata ttcaacattt gtacagagaa cttacgacca gatcacacag
     1621 gatctgtgca tcgatagcaa tgcagcaacg atcgtgactt tctggggatc tgtttatgga
     1681 atgaatgatg ttactcatct tggattacag gatattccaa tgatgagcaa tattccaaac
     1741 ttagtatacc tcgcaccaac aacaaaagaa gaatatctgg caatgttgga ctggagtatc
     1801 gatcaggatc aatatccagt cgcgatcaaa cttccaggtg gagaaatgat ctctgacaga
     1861 aaaaaaataa caaaagattt cagtcagtta aacaaatatg aagtcacaga aaaaggaagc
     1921 aaagttgcag tcattggact tggaacattc tacaatatgg gatgtgaagt tgcaaaagca
     1981 gtagaagaaa agacaggaac aaaagcaact gtgataaatc catattatat tactggaatt
     2041 gatgaagtat tattagaaga tctaaagaaa gatcatgatg tagttattac attagaagat
     2101 ggatttttag aaggaggatt cggagaaaag attgcacgat tctatgggga ttctgatgtg
     2161 aaagtgctga attatggcgt gaagaaggaa ttgttagatc gatatgatgt taccgaactc
     2221 cttgagaaga atcatttgac ggttcagcag attgtggggg atcttaaatt ttaatttgta
     2281 tgagttgaag gtcatcgagt atttagagaa aaaatgaaag atagcatgtg agcaacacga
     2341 attccattga cggagttttg acagaacgct tatccgctgg atctcctgcg attctatcaa
     2401 aactcctatg gaattcgatc gttgctcaca tgctatcttt cattttttct ctaaatacct
     2461 gaagactttc aatcataaaa ataagaaaat tcaggctgat ggatgtatgt taaacattat
     2521 tgtttttcaa tttttgtagt ggtagacaga tgtttattat aaatgctaat atagtaaaat
     2581 attcaccccc accaataact tcagttcatc aaactaccct acaaatcggt gcttgtagat
     2641 tttagctaaa cttctgttaa tgaatacaaa aattatcaat taataccata caacatacac
     2701 actgtatagt gatttgaatt acgtaaatta tttgaatatt ataaaaatgg ttatatgtcg
     2761 gcgaccgtag gaacacgcct gcacttagcg agtggatgat cgtaagatca ttcaggagct
     2821 tagtacagct gcgtagtgag tcgagcgaga gcgcgtcgca gacatataac catttttata
     2881 atattccacc aataatttcc gtcatcaaac tacaatttac tcaccaaaac ttattccaat
     2941 cgtcttagcg tccttaatca tctggcactt cggatcaaca tattttaatt ttccggcaac
     3001 ttcagataat ggaacctttt ttgtatcttc atcaacgata ccaaccatat agccatactc
     3061 tttattaagg attaattcag cggcatatgc tccaactctt gtagcaagta cacggtcgta
     3121 agcacaagga gcaccaccac gctgtgtatg tcctggaacg gtgactctga tctcacgatt
     3181 tgttttttct tctagtgcag ctgcaatctc ataagcaaca gaaggatatc ttctttctgc
     3241 aagttttgcc ttatactgtt tcttctttaa cttcgcatct tcttttgaaa tagcaccttc
     3301 agcaactgcg ataatcgaga atttctttcc agctgcaaag cggttttcta caacttcggc
     3361 tactttatca atatcataag gaatttcagg cagcaggatt acgtcagctc caccagcaat
     3421 tcctgattgc agggtaacat gtccgacttt atgccccata acctcgatga taaatacacg
     3481 gctgtgagaa gaagctgttg tatggattcg gtcgatcgta tcagttgcaa tatcaacggc
     3541 actctggaat ccgaatgtca tttcagttcc ccaaagatca ttgtcaattg tctttggaag
     3601 tgttacaaca tttaaacctt cttcacgaag catatttgca gttttgtgag ttccgttacc
     3661 gccaagaact acaagacagt ctaatttata tttacgataa ttatttttca ttgcctcgac
     3721 tttatcaaga ccgttttcat cagggatgcg catctttttg aatggctgtc tggaagttcc
     3781 aagaattgta cctccacggt ttaaaattcc agaaaaatca tctggtgtca tacggtcagc
     3841 aatatcgtag atcagacctt tatatccatc gcggaaacca tagatctcca gatcatttct
     3901 ctttacactt aaacctttta ttacaccacg catggcagca ttcaatgcct gacagtcgcc
     3961 gccactggtt aaaattccaa ttcttaacat gggttgttta cctccggatc attttttaaa
     4021 aataaattaa cataaactaa ctgttatttt accatataat t
//
LOCUS       DAOMUE010000181         4035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6252_length_4035_cov_2.833920, whole genome shotgun sequence.
ACCESSION   DAOMUE010000181 DAOMUE010000000
VERSION     DAOMUE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6252_length_4035_cov_2.833920"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(381..1817)
                     /gene="purB"
                     /locus_tag="PLC71_08025"
     CDS             complement(381..1817)
                     /gene="purB"
                     /locus_tag="PLC71_08025"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRM02624.1"
                     /translation="MFMNDRYQSPLSERYASKEMQYIFSPDKKFKTWRKLWIALAETE
                     KELGLDITQEQIDELKAHAEDINYDVAKEREKLVRHDVMSHVYAYGVQCPKAKGIIHL
                     GATSCYVGDNTDIIIMTEALKLVRVKLLNVINELSKFAMKYKDLPTLGFTHFQPAQPT
                     TVGKRASLWLMDLVYDLEDLDHVIANMRLLGSKGTTGTQASFLELFEGNHETIKKIDG
                     MIAKKMGFDKCQPVSGQTYSRKVDSRVINVLSQIAQSAHKFSNDIRLLQHLKEVEEPF
                     EKNQIGSSAMAYKRNPMRSERIASLANYVMSDAMNPALVASTQWFERTLDDSANKRLS
                     VPEGFLAIDGILDLYLNVVDGLVVYPKVIESRLRSELPFMATENIMMDAVKAGGDRQE
                     LHEKIRVHSMAAGKVVKEEGKANDLLERIAADPAFGMNMEQLQAIMKPENFVGRAPQQ
                     TEEFVNEVINPILEENKEVLGLTAEINV"
     gene            complement(1828..3255)
                     /gene="purF"
                     /locus_tag="PLC71_08030"
     CDS             complement(1828..3255)
                     /gene="purF"
                     /locus_tag="PLC71_08030"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRM02625.1"
                     /translation="MSRENFDKLGEECGVFGIYDFDGNDVASTIYYGLFALQHRGQES
                     CGIAVSDTNGPKRNVTSQKGMGLCNEVFTPDSLQALKGDIGVGHVRYSTAGESVPENA
                     QPLVLNYVKGTLGLAHNGNLINAIELREELEKTGAIFQTTIDSEVIAYHIARERVNTS
                     SIEKAVARAMQKIKGAYSLIVMSPRKLIGARDPYGFKPLCIGKRDNAYVLASESCALD
                     TIGAEFIRDVNPGEIVTITKDGIQSDCDLCLPKEKTARCIFEYIYFARPDSEIDGVGV
                     YHSRIQAGRYLAQDSPVEADLVVGVPESGNAAALGYSLESGIPYGTAFVKNGYVGRTF
                     IKPGQSSRESSVQIKLNVLKEAVKGKRVIMIDDSIVRGTTSDRIVKMLRDAGATEVHV
                     RISSPPFLWPCYFGTDIPEREQLIAYNRSINEICEVIGADSLGYLGEERLSQMVQGLP
                     ICKGCFTGEYPMKPPTRDIRGNFER"
     gene            complement(3356..>4035)
                     /locus_tag="PLC71_08035"
     CDS             complement(3356..>4035)
                     /locus_tag="PLC71_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRM02626.1"
                     /translation="ELGFNMNLEPVADVLTNKNNTEIGDRSFGTDSKKVADIITTLVK
                     NMQKQQISATLKHFPGSGQTGGDTHRGSTETDQTINTLRDTDFKPFKAGIKAKADAVM
                     VSHLMLSNVTDEKEPSSLSSRVVSDILRDELEYKGVIMTDAMNMKAITDNYSSGEAAV
                     KAIQAGVDLIVMPDNYKEAYKAIKKALKSGKIKESRIDKSVRRIIYTKLKRGEIPPDT
                     TLLKENQ"
BASE COUNT          970 a          916 c          680 g         1469 t
ORIGIN      
        1 cataataaac ggaactataa gtaatccaac tagtatgccc cattgtgtct ttttatattg
       61 aattcttttc cagatttttt ctatcaacgg tgttaatata taacaaatca ttaacgttgt
      121 cataaaccag gtatgatctg cacctaatac tcctacatgc attccttgta taaaaaacta
      181 taatccttct atatatttta ttataaaatt tattatagta ccttttttct taatttccta
      241 tatctactcc tgctcatttc ctgctttttg gtaatttttt cttaaaatac agcaaaaaga
      301 gatggaaaaa tctatattgt tttgatcttt ccatctcttt ttatgttaaa attttatctt
      361 ttcatcaaga ttctatcttt ttatacgttg atctcagctg ttaatccgag tacttcttta
      421 ttctcttcca gaattggatt gatcacttca ttcacaaatt cttctgtctg ctgtggtgca
      481 cgtcctacga agttctctgg tttcatgatc gcctgtaact gctccatgtt cataccaaat
      541 gctgggtctg ctgcgattct ctctaacaga tcgtttgctt ttccttcttc tttgacaact
      601 tttcctgctg ccatagaatg tacacggatc ttctcatgaa gttcctgtct gtctccacca
      661 gcttttactg catccatcat gatattctct gttgccatga atggaagttc gcttcttaaa
      721 cggctttcga taaccttagg gtatacaact aatccgtcta caacgtttaa gtaaaggtct
      781 aagataccat cgattgctaa gaatccttct ggtacagata aacgtttgtt ggctgagtca
      841 tctaatgttc tttcaaacca ctgtgtagat gctactaatg ctgggttcat ggcatctgac
      901 attacgtagt ttgctaagga agcgattctt tcacttctca ttgggtttct cttatatgcc
      961 attgcagagg atccgatctg gttcttctca aatggttctt ctacttcttt taagtgctga
     1021 agaagtctga tgtcgtttga gaacttatga gcagactgtg cgatctggct taatacgttg
     1081 atcacgcggg aatcaacttt tctagagtat gtctgtccag atactggctg gcatttgtca
     1141 aatcccatct tcttggcgat cattccatca atcttcttga tcgtttcgtg gtttccttcg
     1201 aataactcta agaaacttgc ctgtgttcct gttgttcctt ttgatcctaa taatctcatg
     1261 ttagcgatca cgtgatcaag atcttctaga tcatatacga gatccatcag ccaaaggctt
     1321 gctctcttac ctactgttgt aggctgtgct ggctgaaaat gtgtaaatcc aagtgttgga
     1381 agatctttgt atttcattgc gaatttggat aattcgttga tcacatttaa taattttact
     1441 ctgacaagct ttaatgcttc tgtcatgatg atgatgtctg tgttatcacc aacatagcag
     1501 gatgttgctc ctaaatggat gattcctttt gcctttggac attgtactcc gtatgcatat
     1561 acatgagaca taacatcatg acggactaat ttttcacgtt cttttgctac atcgtagttg
     1621 atgtcttctg catgtgcttt taattcgtcg atctgttcct gtgtgatatc aagtcctaat
     1681 tctttctctg tctcagcaag tgcgatccat aactttctcc atgtcttaaa cttcttgtct
     1741 ggagaaaaga tgtactgcat ctctttgctt gcataacgtt ctgataatgg gctctggtaa
     1801 cggtcgttca taaaaatctc cttatcctta tctttcaaaa tttccacgaa tatctcttgt
     1861 tggcggtttc attggatact cacctgtgaa acatccttta cagatcggta atccctgtac
     1921 catctgtgaa agtctctctt ctcctaagta tcctaaacta tctgctccga tcacttcaca
     1981 gatctcgttg atcgatctgt tatatgcgat cagctgttct ctctctggaa tatcagttcc
     2041 aaagtagcat ggccacagga atggtgggga actgattctt acgtgaactt ctgtcgctcc
     2101 tgcatcgcgt aacatcttca caatgcggtc acttgttgtt cctcgtacga tggaatcatc
     2161 gatcatgatg actctctttc ctttgactgc ttcttttaaa acatttaact tgatctgcac
     2221 gcttgattca cggctgctct gtcctggctt gatgaaagtt cttccaacat aaccgttctt
     2281 gacaaacgcg gttccgtatg ggattcctga ctctaaggaa tatccaagag ctgctgcatt
     2341 tcctgattct ggtactccga caaccaagtc tgcttctacc ggtgaatcct gtgcgaggta
     2401 tcttcctgcc tgaattcttg agtggtatac cccaactccg tcaatctcac tgtctggtct
     2461 tgcaaaataa atgtattcaa agatacatct tgctgttttc tcctttggaa gacataaatc
     2521 acagtctgac tgaattccat cctttgtgat cgttacgatc tctcctggat tcacgtcacg
     2581 gataaattct gctccgatcg tatctaatgc acagctttca gatgctaata cataagcatt
     2641 atctctctta ccgatacata atggcttaaa tccatatgga tctcttgccc cgatcagttt
     2701 ccttggtgac ataacgatca gggaatatgc acctttgatc ttctgcattg ctctggctac
     2761 tgccttctca attgatgatg tattcacgcg ttctcttgcg atatgatatg cgataacttc
     2821 ggaatcgatc gttgtctgga aaatagctcc tgtcttttct aattcttcgc gaagttctat
     2881 tgcattgatc agattaccat tatgtgcaag tcctaaagta ccttttacat aatttaaaac
     2941 cagtggctgg gcattctcag gaacactctc tcctgctgtt gaatacctta catgccccac
     3001 accgatatct ccctttaatg cctgaaggga atctggtgta aacacttcat tacataatcc
     3061 catacctttt tgagatgtga cattacgttt tggaccattc gtatcagaaa ctgcaattcc
     3121 gcagctttcc tgacctcgat gctgaagtgc aaacaatcca taatagatcg ttgacgctac
     3181 atcattgcca tcgaaatcgt agataccgaa aactccacat tcttctccca acttatcaaa
     3241 gttctctctg ctcatcgtga tcttttgacc tttctgttat acaatcttta gtttcctttg
     3301 taaaatacac atttttgata gtaacatatt cattttaaat ctgcaagcat ccctgttatt
     3361 gattctcttt taataatgta gtatctggag ggatctctcc tcgcttaagc ttcgtatata
     3421 tgatccttct tacagattta tcgattcttg attctttgat cttgccactt tttagtgcct
     3481 tcttaattgc cttgtatgct tctttatagt tatccggcat cacgatcagg tcaactcctg
     3541 cctgaattgc tttcactgct gcttctccgg aactatagtt atctgtaatc gctttcatgt
     3601 tcatcgcatc ggtcatgata acgcccttat attcaagttc atctcttaga atatctgata
     3661 caactctgct gctcaggctt gaaggctcct tctcatctgt cacattagac agcatcagat
     3721 gtgacaccat cactgcatca gcttttgcct tgatccctgc tttaaatggt ttaaaatctg
     3781 tatctcttaa tgtattgatc gtctgatcgg tctctgtgct tccgcggtgt gtgtctcctc
     3841 cagtctgtcc acttcctgga aaatgcttta atgtcgctga gatctgttgt ttctgcatgt
     3901 tctttaccag tgttgtaata atatcagcaa ctttctttga gtctgtgcca aagcttcggt
     3961 ctccgatctc tgtgttattc ttattcgtta aaacgtctgc gactggttcc agattcatgt
     4021 taaatccaag ctctt
//
LOCUS       DAOMUE010000182         4009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6307_length_4009_cov_2.306019, whole genome shotgun sequence.
ACCESSION   DAOMUE010000182 DAOMUE010000000
VERSION     DAOMUE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6307_length_4009_cov_2.306019"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PLC71_08040"
     CDS             <1..650
                     /locus_tag="PLC71_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HRM02627.1"
                     /translation="TGRKLIASRCAKFFKDGDFVNLGIGVPLMCVNYLPEGVDLWLEA
                     EIGTVGSGPTPSWDEADIDVIDAGGQPASVIKGGSVYDHEMSFAFIRGGHIDAAVLGT
                     LQVDQEGNIANWTIPGKMVPGMGGAMDLCAGVKKIIVATDHCEKSGKSKILKKCTLPL
                     TGVHCVTDIVTERCYFEVTKDGLVLRELAEEYSVEDILACTEADVIVPDEIGVMS"
     gene            complement(733..2124)
                     /locus_tag="PLC71_08045"
     CDS             complement(733..2124)
                     /locus_tag="PLC71_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRM02628.1"
                     /translation="MNQEKNKGAEYDFYGKLPLQKAILLGLQHVFAMFVGNLTPVLII
                     TGACGIASGTEFADVQIALLQNAMLIAGIVTLIQLYAIGPIGGKVPIIMGTSSGFIGV
                     CSSVASVMGGGILGYGAIMGASIIGGLFESVLGFMLKPLRRFFPAVVTGTVVLSIGLS
                     LIGVGVNSFGGGNSANDFGSIENLLLGTIVLVIIIALKHGTKGITSASSVLIGIIAGY
                     IIAGIMGVVLPTTAVNADGVEYTKAWVLNWDKVAQAGWFAIPKFMPVKIIFDARAIVP
                     VLIMFIVTAVETVGDISGVIEGGMDREATDSELSGGVVCDGIGSTIAAFFGVLPNTSF
                     SQNVGLVTMTKIVNRFALATGAIFLILCGLFPKLAALISIMPQSVLGGAAVMMFSSIV
                     ISGIQLITKEPLTMRNITVVSVALGLGYGIGANSTVLSGLPEGIQLIFGGSGIVPAAL
                     VAIILNIALPKED"
     gene            2316..3080
                     /locus_tag="PLC71_08050"
     CDS             2316..3080
                     /locus_tag="PLC71_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HRM02629.1"
                     /translation="MISLGLDSGSSMTKAVLLEDMKIIDTKMIPTSISPRKSLEALYR
                     EIKKQHKEIDVIVTTGYGRNLLDQADFKITEITCHAAGAFFMNPDCDTIIDIGGQDSK
                     VITIDDLGCVTDFLMNDKCAAGTGRFLEMTMKRVESEIGRIDTFVKDAKPAKINSMCA
                     VFAESEIVGLLAQDIAPGDIVLGCVESICQRTASFAQKLAPANPKVFFSGGLAQFDVI
                     KRSLASHLGVKEVATDPLCQYTGAIGAAVLGIRKIK"
     gene            3110..>4009
                     /locus_tag="PLC71_08055"
     CDS             3110..>4009
                     /locus_tag="PLC71_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002564916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HRM02630.1"
                     /translation="MVELKKELPEIFEEFAEQRKNSFLAIKEVKEKEIPVVGVFCTYL
                     PREIPNAMGAAVVGLCSVSDETIPDAEKDLPRNLCPLIKSSYGFAKTDKCPFFYFSDL
                     VVGETTCDGKKKMYEMLADFKPVHVVELPNCQTEDGIQLYKKELLRFKKVLEDKFETT
                     ITDEAVLHQIKLRNGINKALRRLQYVMANDPAPVNGLDVINTVYGSGFDINTEGLEDR
                     INAVTDKIEKEYTEGKNIGKKPRILVTGSPSGGAALKVIRAIEDNGGVVVCFENCTGM
                     KPLAMVDEENPDVYDALARKYLNI"
BASE COUNT         1326 a          730 c          851 g         1102 t
ORIGIN      
        1 tgacaggaag aaagctgatc gcatcacgat gtgcaaagtt ttttaaagat ggagattttg
       61 taaatcttgg tatcggagtt ccgctgatgt gtgtaaatta tctcccagaa ggtgtggatc
      121 tgtggctgga agcagagatc ggaaccgtcg gaagtggacc aaccccaagc tgggatgaag
      181 cagatattga tgttattgat gctggcggac agcctgcatc tgtgatcaaa ggtggaagcg
      241 tgtatgatca tgagatgtct tttgcattta tacgtggcgg acatattgat gcagcagttc
      301 ttggtacact tcaggtagac caagaaggca atatcgctaa ctggacgatt ccagggaaga
      361 tggtgccagg aatgggtggt gccatggatc tgtgtgccgg agtgaagaag atcatcgtag
      421 caacagacca ttgtgaaaag agtggaaagt ctaagatatt aaagaaatgt acacttcctt
      481 tgacgggagt tcattgtgta acagatatcg taacagaacg ctgctatttt gaagttacaa
      541 aagatggtct cgtgttaagg gaacttgcag aagaatatag tgtagaagat atccttgcat
      601 gcacagaagc tgatgtgatc gtaccggatg aaattggtgt tatgagctga taatagacac
      661 aggaaagcat atagaaaaac agcactgtac aggaataaaa ttcccaatgc agtgctgttt
      721 ttattagttt atttaatctt cttttggcaa tgcaatattt aaaataattg caacaagtgc
      781 cgcaggtaca ataccagatc ctccgaagat caactgaatt ccttcaggta gtccagaaag
      841 aactgtgctg tttgcaccga ttccatatcc aagtccgaga gctacagata cgacagtaat
      901 atttctcatt gttagtggtt cttttgtgat cagctgaata ccactgatta cgatagaaga
      961 gaacatcata acagctgctc cacctaatac gctctgtggc atgatagaga tcaatgcagc
     1021 aagttttgga aataatccac acaggattaa gaagattgct cctgttgcaa gtgcaaaacg
     1081 gtttacgatc tttgtcatag taacaagacc aacattctgg ctgaatgatg tatttggtaa
     1141 aacaccaaag aatgctgcga tcgtagatcc gataccatca cagacaacac caccagaaag
     1201 ctcagaatct gtagcttcac gatccatacc accttcgata actccggaaa tatccccgac
     1261 tgtttctaca gcagttacga taaacatgat aagtacaggg acgatcgctc ttgcatcaaa
     1321 aatgattttt acaggcataa attttggaat ggcaaaccat ccagcctgag caactttatc
     1381 ccagtttaac acccatgctt ttgtatactc tacaccatct gcattgacag cagttgtagg
     1441 aagtacaaca cccatgattc ctgcgataat gtatcctgcg ataattccga tcagtacaga
     1501 agaagcactt gtgatacctt tcgttccatg ttttaatgca atgatgatca caagaacaat
     1561 tgttccaagt aacaagttct cgattgaacc gaaatcatta gcagaatttc ctccaccaaa
     1621 agaattcaca ccaacaccga tcagtgataa tccgatggaa agtacaaccg taccagttac
     1681 gacagctggg aaaaatcttc gaagtggttt taacataaat cctaatacag attcaaataa
     1741 cccaccgata atggaagcac ccatgatcgc accatatcca aggattccac cacccataac
     1801 agatgcaaca ctgctgcaga caccgataaa tcctgaactc gttcccatga tgattggaac
     1861 ttttccaccg atcggtccga tcgcgtacaa ctggatcagt gtaacaattc cggcaattaa
     1921 cattgcattt tgtaaaagcg caatctgaac gtctgcaaat tcggttccag aagcaatacc
     1981 acaagcccct gtaatgataa ggactggagt aaggttacct acgaacatag cgaatacatg
     2041 ctgcagacca aggagaatcg ctttttgcag cggaagtttc ccgtaaaagt cgtattccgc
     2101 acctttgttt ttctcttgat tcatttttct ttcgtctcct tttttttaat gcatgatgag
     2161 cagttttata ctgctatttt tcacagcata ctattataaa ggatttgaat gaaaatgtat
     2221 agttcttttt tttaagcatg tttttttaca ttgcaaccac accattttca tgataaaatt
     2281 tacccgaaaa actttccaaa aggagtcaat gtacagtgat aagtttagga ttagattctg
     2341 gttcgtctat gacaaaagcc gttttgttag aagatatgaa gataatagat acaaagatga
     2401 tcccaacatc gatcagtcca aggaaatctc tagaagctct ttacagagag ataaaaaaac
     2461 aacataaaga aatagatgtt attgtaacaa ctggatatgg aaggaatctg ttagatcagg
     2521 cagatttcaa gatcacggag attacttgcc atgcagctgg agcatttttt atgaatccag
     2581 attgtgacac gatcatagat atcggtgggc aggatagcaa agtgatcacc attgacgatc
     2641 ttggatgtgt cacagatttt ttaatgaatg ataaatgtgc tgctggaact ggacgattcc
     2701 ttgagatgac gatgaaacga gtcgaaagtg agattggaag aatcgacact tttgtaaaag
     2761 atgccaagcc tgcgaagatc aacagtatgt gtgctgtatt tgcagaaagt gaaatcgttg
     2821 gattattggc acaggatatt gcacctggtg atattgttct tggttgtgta gaatctattt
     2881 gtcagcgaac cgcttcattt gcacagaaat tggcgccagc gaatccaaaa gtatttttct
     2941 ctggtggact agcacaattt gatgtgatca aacgatcatt agccagtcat ttaggagtga
     3001 aagaagtagc aacagatccg ttatgtcaat acacaggagc aattggagct gccgttttag
     3061 gcatccgcaa aataaaatga taatttttac ttacaggagg attcaaacaa tggttgaatt
     3121 aaagaaagag ttaccagaga tttttgagga atttgcagaa cagagaaaga attcctttct
     3181 cgcgatcaag gaggttaaag aaaaagaaat acctgttgtc ggtgtatttt gtacctatct
     3241 tccaagagag attccaaatg ccatgggtgc agcagttgtc ggattatgtt ccgtatcaga
     3301 tgaaacaatc ccagatgcag aaaaagatct gccaagaaac ctatgtccat tgatcaaatc
     3361 aagttatgga tttgccaaaa cagataaatg tccattcttc tatttttcag atttggttgt
     3421 aggagaaaca acatgtgatg gaaagaaaaa aatgtacgaa atgcttgctg attttaaacc
     3481 agttcatgtc gtagaacttc caaactgtca gacagaagat ggaatccagt tatataagaa
     3541 agaattactt cgtttcaaga aagttctaga agataaattt gagacaacga tcacagatga
     3601 ggcagttctt catcagatca agttaagaaa tggaatcaat aaagcattaa gacgtcttca
     3661 atatgtaatg gcaaatgatc ctgcaccagt caatggatta gatgtgatca atactgtata
     3721 tggaagtggt tttgacatta acacagaagg tcttgaagac agaatcaatg cggtaacaga
     3781 taaaattgaa aaagaatata cagaaggaaa aaatataggg aaaaaaccaa ggatccttgt
     3841 aaccggatct ccatctggcg gtgcggcctt gaaagtcatt cgtgcaattg aggataatgg
     3901 tggagttgtc gtatgttttg aaaactgtac tggaatgaaa ccgttagcaa tggtagatga
     3961 agagaatcca gatgtatatg atgcactcgc aagaaaatat ttaaacatt
//
LOCUS       DAOMUE010000183         3999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6328_length_3999_cov_2.353448, whole genome shotgun sequence.
ACCESSION   DAOMUE010000183 DAOMUE010000000
VERSION     DAOMUE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6328_length_3999_cov_2.353448"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1730)
                     /gene="groL"
                     /locus_tag="PLC71_08060"
     CDS             complement(105..1730)
                     /gene="groL"
                     /locus_tag="PLC71_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009267873.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRM02631.1"
                     /translation="MAKEIKYGIEARKALEAGVNQLADTVRVTIGPKGRNVVLDKSFG
                     APLITNDGVTIAKEIELEDPFENMGAQVVKEVATKTNDVAGDGTTTATVLAQAMINEG
                     MKNLAAGANPIILRKGMKKATEAAVEAIAEMSSKVTGRDQIAKVAAISAADEEVGELV
                     ADAMEKVSNDGVITIEESKTMKTELDLVEGMQFDRGYLSAYFSTDMEKMVAELDDPYI
                     LITDKKITNIQEILPLLEQIVQSGKKLLIIAEDIEGEALTTLIVNKLRGTFSVCAVKA
                     PGYGDRRKAMLEDIAILTGGTVISEEVGLELKDATLDQLGRAKSVKVEKENTVIVDGE
                     GDKDAIQARLGQIKAQIEETTSEFDKEKLQERLAKLSGGVAVIRVGAATETEMKESKL
                     RLEDALAATKAAVEEGIIFGGGSAYIHASKKAAEKVADLEGDEKTGANIILKALEAPL
                     FQIAVNAGLEGAVIVNKVKEAEIGKGFDALHGEYVDMVEKGIIDPAKVTRSALQNATS
                     VASTLLTTESVVANIKEEAPAMPAGAPAGMGMM"
     gene            complement(1793..2065)
                     /locus_tag="PLC71_08065"
     CDS             complement(1793..2065)
                     /locus_tag="PLC71_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRM02632.1"
                     /translation="MKLTPLGDRVVLKQLEAETTTKSGIVLTTATQEKPQEAEVVAVG
                     PGTEDVKMEVSVGQKVIYSKYAGTEVKLEDEEYIIVKQNDILAVVE"
     gene            complement(2238..2723)
                     /locus_tag="PLC71_08070"
     CDS             complement(2238..2723)
                     /locus_tag="PLC71_08070"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM02633.1"
                     /translation="MKNIVLARVDDRLIHTEILTLWVPEVRANRIIIVDDVVAKDKFR
                     SKVIKEMAPQGLIIHVYGIDRATEKLKEAPSFDGERVIVIAESPLVFEELIKRGIKIN
                     QLNIGGMGIRGERKAVARRVACDTMEMEAIHELIKKHVHVYFQTVPTRSSEEAKKYIK
                     E"
     gene            complement(2720..3151)
                     /locus_tag="PLC71_08075"
     CDS             complement(2720..3151)
                     /locus_tag="PLC71_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM02634.1"
                     /translation="MREKRGKLMKVIVVSHGSYARGLVDTAQMIAGEQEDLEAFGLEP
                     EESVDTLREKIRESIEQTSEGEEVLILTDLFYGSPFNTVISLMSEYDLYHVTGINLPL
                     MMEVVMGRYAGKSAQEVCKDLLKAAPETVKDVRELYKEVEE"
     gene            complement(3180..>3999)
                     /locus_tag="PLC71_08080"
     CDS             complement(3180..>3999)
                     /locus_tag="PLC71_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRM02635.1"
                     /translation="VDGWDDPRLVSLSALRRRGYTPESIKKFMELVGVAKSHSSVDSA
                     MLEYCIRDDLKLKRPRMAAILDPIKLVITNYPEGEGELVDVPNNQENEEMGTRQVPFS
                     RELYIEREDFKIEKPKKYRRLYVGNEVRLMNAYFVTCTGYDVDEDGNVTCVYATYDPE
                     SRGGNSPDGRKVRGTIHWVSVPTAKKAEIRLYENIVDEEKGVYNEDGSINVNPNSLTV
                     IKEAYVEPELEKYDKEDSFQFMRTGYFCLDSKDSTKEHMVFNRIVSLKSSYKPN"
BASE COUNT          936 a          945 c          604 g         1514 t
ORIGIN      
        1 acctcaaaac tctttcgaat tctgaggtgt ttttatattt aatctttgag ttgcttcgct
       61 tgtcagcttg ttttcactaa gtgttcaatg ccagtgttca atgcctacat cattcccatt
      121 ccggctggtg ctcctgctgg cattgctggt gcttcttctt tgatattcgc aacaactgat
      181 tctgttgtta ataatgtaga agctacggat gttgcattct gtaatgcgct tcttgttact
      241 ttggctggat cgatgattcc tttttctacc atgtctacgt attctccatg taatgcatcg
      301 aatccttttc cgatttctgc ttcttttact ttattgacga tcacagctcc ttcaagacct
      361 gcattgactg cgatctggaa taatggtgct tctaatgctt tgaggatgat gtttgctcct
      421 gttttctcgt ctccttctaa gtctgctact ttctcagctg ctttcttaga tgcatggata
      481 taagcagatc ctccaccgaa gatgattccc tcttctacag cggcttttgt tgctgctaag
      541 gcatcttcca gacgtaattt gctttctttc atctctgttt ctgttgctgc tccaacacgg
      601 atcactgcaa ctccaccaga taatttagct aatctttcct gtaatttttc tttgtcgaat
      661 tcagatgttg tttcttcgat ctgtgctttg atctgtccaa gacgtgcctg gattgcatct
      721 ttatctcctt caccgtctac gatgaccgtg ttttcttttt caactttgac agattttgca
      781 cgtcctaact gatctaatgt tgcgtctttt aattccaggc caacttcttc agagatcact
      841 gtaccacctg ttaagatagc gatatcttca agcattgctt ttcttctgtc tccatatcca
      901 ggtgctttca ctgcacatac agagaatgtt ccacgtaatt tgttaacgat cagtgttgtt
      961 aatgcttcac cttcgatatc ttcagcaatg atcagtaatt tcttaccact ctgtacaatc
     1021 tgctctaata atggaaggat ttcctgaata ttagtgatct tcttgtctgt gatcagaata
     1081 tatggatcat ctaattcagc aaccattttt tccatatctg ttgagaaata tgctgataaa
     1141 tatccacgat caaactgcat tccttctact aaatctaatt ctgttttcat tgttttagat
     1201 tcttcgatcg tgataactcc gtcgttagat accttttcca tagcatctgc tactaattct
     1261 ccaacttctt cgtctgctgc agagattgct gctactttcg cgatctgatc tcttcctgta
     1321 actttgctac tcatctcagc aattgcttca acagctgctt ctgtcgcttt cttcattcct
     1381 ttacgaagaa tgattgggtt tgctcctgct gctaagtttt tcattccttc gttgatcatt
     1441 gcctgtgcaa gaactgtagc tgttgttgta ccatctccag caacatcatt tgtcttagtt
     1501 gctacttctt tgactacctg agctcccata ttctcaaatg gatcttctaa ttcgatttct
     1561 tttgcgatcg ttacaccgtc atttgtgatc agtggtgcac caaaagattt gtctaataca
     1621 acattgcgtc cttttggtcc gattgtaact cttactgtat ctgctaactg gttaactcct
     1681 gcttccagtg cttttcttgc ttcaattccg tatttaatct cttttgccat gatcttcatt
     1741 acctccgact aaatttttta atgaaattaa ataattgtgt attataataa aactattcta
     1801 caactgctaa aatatcattc tgttttacga tgatatattc ttcatcttct aattttactt
     1861 ctgttcctgc atatttagaa taaataactt tctgtcctac agaaacttcc atcttcacat
     1921 cttctgttcc aggtccaact gcaacaactt ctgcttcctg tggtttttcc tgtgttgctg
     1981 ttgtaaggac gattcctgac ttagttgttg tttctgcttc cagctgtttt aatacaactc
     2041 tatctcctaa tggtgttaac ttcataatga tattacctcc taaatatatt agaattattg
     2101 ttctttttat cttttgttag cactcgctta tatcgagtgc taactctatg ttctttagta
     2161 taacaccaga caaaaaaaat gcaaccccct ttttataaaa attctataaa ctgagattgc
     2221 attctttggt attttactta ttctttgatg tatttttttg cttcttctga acttcttgtt
     2281 ggaactgtct ggaaatatac atgtacatgt tttttgatca gttcatgaat agcttccatc
     2341 tccatggtat cacacgcaac tcttcttgca actgctttac gttctccacg aattcccata
     2401 cctccgatat taagctgatt gatcttgatc cctcgtttga tcaattcttc aaataccagt
     2461 ggggattctg caattacgat cacccgctct ccatcaaagg atggagcttc ttttaatttc
     2521 tccgttgcac gatcgatccc atatacatga attataaggc cttggggcgc catctctttg
     2581 atcactttgc ttcggaattt atccttcgcc acaacatcat ctacaataat gatcctgttc
     2641 gctctgactt ccggcaccca taatgtcaaa atctccgtat gtatgagcct gtcatcaact
     2701 ctcgctaata caatgttttt cattcttcta cctccttata taattcacgc acatctttca
     2761 ctgtctctgg tgctgcctta agaagatctt tgcacacctc ttgtgcgctc tttcccgcgt
     2821 aacgtcccat aactacttcc atcatcaacg gaagattgat tccagttacg tggtagagat
     2881 cgtattctga cattaaagaa attactgtat taaacggact tccataaaac agatctgtta
     2941 atataaggac ttcttctcct tctgatgtct gttcaatact ctctctgatc ttctctctca
     3001 aagtatccac tgattcttct ggttcaagcc caaatgcctc aaggtcttcc tgttcccctg
     3061 cgatcatctg tgctgtatcc acaagtcctc ttgcataact tccatgagat accacgatta
     3121 ccttcatcaa ttttcctctc ttctctctca tcacatcttg cctgttacgg caaatttgat
     3181 taatttggtt tgtaagaact ctttaagctt acaattctat taaataccat gtgttctttt
     3241 gtagaatctt tggaatctaa acagaaatat cctgttctca taaactggaa actgtcttcc
     3301 ttatcatatt tctctaactc aggctctaca tatgcttcct tgatcacagt cagtgaattt
     3361 ggattcacat tgatacttcc atcttcatta tatacacctt tttcttcatc tacaatgttt
     3421 tcatacagac gaatttctgc ttttttcgca gttggtacag atacccaatg gatcgttccg
     3481 cgaacctttc ttccatctgg actgtttcct cctctggatt ctggatcata tgttgcatat
     3541 acacaggtta cattgccgtc ctcatctaca tcatatcctg tacatgttac aaaatatgca
     3601 ttcatcagac gcacttcatt acctacatat aaacgacggt atttcttagg cttctcaatc
     3661 ttgaaatctt ctcgttcaat atataattct cttgaaaatg gtacctgtct tgttcccatc
     3721 tcttcgtttt cctgattatt tggaacgtca acgagttccc cttctccttc tggatagttt
     3781 gtgatcacca gtttgatcgg atctaagatc gctgccatac gtggacgttt taatttcaga
     3841 tcatcacgga tgcagtattc aagcatcgct gaatctacag agctgtggct ctttgcaact
     3901 ccgacaagtt ccataaactt cttgatggat tctggtgtat atcctcttct tctaagtgct
     3961 gataatgata ccagacgtgg atcatcccat ccatctacg
//
LOCUS       DAOMUE010000184         3997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6330_length_3997_cov_2.210553, whole genome shotgun sequence.
ACCESSION   DAOMUE010000184 DAOMUE010000000
VERSION     DAOMUE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6330_length_3997_cov_2.210553"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2272)
                     /locus_tag="PLC71_08085"
     CDS             complement(<1..2272)
                     /locus_tag="PLC71_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HRM02636.1"
                     /translation="MKQTNQIPGLYDPSFEHDNCGIGACVNIKGVKNHQTVDNALKIV
                     ETLEHRAGKDAAGETGDGVGIMLQISHKFFSKACKAANINIGKEREYGIGMFFFPNDE
                     LRLRQAKKMFEIIVEKEGMTFLGWREVPTTPTVLGKKALECMPKILQGFVAKPADVEK
                     GLDFDRKLYIARKVFEQSNEESYVVSLSSRTIVYKGMFLVGQLRTFFNDLQDSDYESA
                     IALVHSRFSTNTTPSWEKAHPYRFIVHNGEINTIRGNADKMLAREENMESEYLKNEMH
                     KLIPVVNPNGSDSARLDNTLEFLVMSGMDLPHAIMITIPEPWENNKVISQEKRDFYQY
                     YATMMEPWDGPASIIFTDGDQVGAVLDRNGLRPSRYYITDDDQLILSSEIGVLDIDPT
                     KIVVKERLHPGKMLLVDTIKGKVIADDVVKEKYAEKEPYGEWLDSNLLELKDLKIPNE
                     PVPTYGAEEREKLMKAFGYTYEDIRTAILPMALNGSEAIAAMGNDTPLAVLSNRHQPL
                     FNYFKQLFAQVTNPPIDAIREELVTSTTVYVGKEGNILDEKPENCRVLKVHNPILTDT
                     DLLKIKSMNKKGFEVVELPITYYKNTSLEKALDRLFVEADRAYRDGANIIILSDRGVD
                     ENHVAIPSLLAVSAMHQYLIKTKRRTSVALILESGEPREVHHFATLLGYGACAVNPYL
                     ALQGIKKLIDENMLQKDYYAAVEDYTNAILHGIVKIASKMGISTLQSYQGSQIFEAIG
                     INSDVIEKYFTNTVSRV"
     assembly_gap    2608..2617
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2629..3951
                     /locus_tag="PLC71_08090"
     CDS             2629..3951
                     /locus_tag="PLC71_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM02637.1"
                     /translation="MSNLLLRFAIPSIIAMVVSALYNMVDQFFIGRSVGMLGNAATNV
                     AFPLVITCTAIALMCGIGGAANFNLCMGQQEKEEAASFAGNAITMLFSLGMILCIITR
                     LFLKPMMILFGATSSVLDYSLIYTGITSLGFPFLILTTGGTNLVRADGSPKFSMTCTL
                     TGAIINTILDPTLIFGFHMGIAGAAWATVIGQVVSGIMVILYLRKFKTVKLTKEKLRP
                     HPHYIGRIVSLGMAPFFNQVSMMVVQIVMNNVLIRYGAKSSYGSDIPLACAGIITKIN
                     MIFFAINIGISQGLQPIISYNYGARKLDRVREAYLKAAISATIISTVSFICFQLFPRQ
                     IIGIFGSESEAYFRFAEKFFRIFLFCTFINGLQPITANFFTAIGKANRGIFLSLTRQV
                     IFLLPLMIILPIFFGIEGVMFSAPIADGFAAVCAITFAVKELKSFQKL"
BASE COUNT         1075 a          783 c          708 g         1421 t
ORIGIN      
        1 caactctgct gactgtattt gtaaaatatt tttcgatcac atcagaattg ataccaattg
       61 cttcaaagat ctgtgatccc tgataagact gtaatgttga aatacccatc ttagatgcga
      121 tcttaacgat tccatgaagg attgcatttg tataatcttc aacagctgcg taataatctt
      181 tctgaagcat attttcatcg atcagtttct taattccctg taatgcaaga tatgggttca
      241 ctgcacatgc accatatcct aacagtgttg caaaatgatg aacttctctt ggttccccgc
      301 tctctaagat cagtgcaaca gatgtacgtc tctttgtctt gatcagatac tgatgcattg
      361 ctgatactgc taataaagaa gggattgcca catggttttc atcgactcct ctatcagata
      421 ggataatgat attcgctcca tctctgtatg ctctgtctgc ctctacaaac aagcgatcta
      481 aggctttctc aagagatgta ttcttgtagt atgtaatagg aagttctaca acctcaaatc
      541 cttttttatt catagatttg atctttaaca gatctgtatc tgtaagaatt ggattgtgta
      601 cttttaatac gcggcagttc tctggtttct cgtctaaaat attaccttct tttccaacat
      661 aaactgttgt tgatgttaca agttcctcac ggatcgcatc aattggaggg tttgttacct
      721 gtgcaaataa ctgtttgaaa taattaaata atggctgatg acgattggat aatactgcaa
      781 gtggtgtatc atttcccatg gctgcgattg cttcagatcc atttaacgcc attggaagga
      841 ttgctgtacg gatatcttca taagtatatc cgaatgcttt catcaatttt tcgcgttctt
      901 ctgctccata tgttggaact ggttcatttg gaatctttaa atcttttaat tctaacagat
      961 tactgtctaa ccattcacca tatggctctt tctctgcgta tttttctttc actacatcat
     1021 ctgcgatcac ttttcctttg atcgtatcaa ctaacaacat ctttcctgga tgaagtcttt
     1081 ctttcactac gatctttgtt gggtcgatgt caagaactcc tatctcagaa gacaggatca
     1141 gctgatcatc atctgtgatg taatatcttg atggacgaag tccattacgg tcaagtactg
     1201 ctcctacctg atcaccatct gtgaaaatga tagatgctgg tccatcccat ggttccatca
     1261 tcgttgcata atattgatag aaatcacgtt tttcctgaga aataacttta ttattttccc
     1321 atggttctgg gattgtgatc atgatcgcat gcggaagatc cataccgctc ataactaaga
     1381 actctaatgt attatcaagt ctcgcagagt cagatccatt cggatttacg actgggatca
     1441 gtttgtgcat ctcattttta agatattctg attccatatt ttcttctctg gcaagcatct
     1501 tatcggcgtt accacggatg gtattgattt ctccattatg tactataaaa cgatatggat
     1561 gagctttctc ccagcttggt gtcgtattag tactgaatcg tgaatgcaca agtgcgatcg
     1621 ctgattcata atctgaatcc tgaagatcgt taaagaatgt acgaagctgt ccaactaaga
     1681 acataccttt atatacgatc gttctgctgg ataatgaaac aacgtatgat tcttcattac
     1741 tctgttcaaa tacttttctt gcgatatata atttacgatc aaaatcaagt cctttttcaa
     1801 catccgcagg ttttgctaca aatccctgta agatctttgg catgcattct aatgctttct
     1861 taccaagaac tgttggtgtt gtaggaacct ctctccatcc aaggaatgtc attccttcct
     1921 tctcaacgat gatctcaaac atcttcttcg cctgacgaag tctcaattca tcattcggga
     1981 agaagaacat tccgattcca tattctcttt cttttccaat attgatattg gctgccttac
     2041 aggctttgct aaagaactta tgagaaatct gaagcatgat tcctacacca tctcctgtct
     2101 ctcctgctgc atcttttcct gcacgatgct ctaatgtctc aacaatcttc agtgcattat
     2161 ctactgtctg atgattcttg actcccttaa tatttacaca ggcaccgata ccgcagttat
     2221 cgtgctcaaa acttggatca tataatccag gaatttgatt tgtctgtttc atatctattc
     2281 atcctttctc gatgtactgt ctcgtgtttt ttcttatcat accgaaattt tccctagttg
     2341 taaagtatga attttcttaa gttgtctgat aattagattt cttaacaatt tatacattta
     2401 ttttctgata atatatcgaa ttatctctta ttttctcgtt tactgaatcc tgtttctcaa
     2461 aaagtttttt aaaaagttgt actattttgt catttatact atactagata acgtccgttt
     2521 cgaccatttt cgatcgtttc gacaatcttt tatattatat atattataga aagaagaggt
     2581 tttatttatt atgaacgaac aaaagacnnn nnnnnnngta ccgagaagat atcaaatttg
     2641 cttcttcgtt tcgctattcc tagtattatc gctatggtcg taagtgcctt atataatatg
     2701 gtagaccagt ttttcattgg acgaagtgtt gggatgcttg ggaatgcagc aacaaatgtt
     2761 gcctttcctt tagttatcac ctgtactgcc atcgccttga tgtgtggaat cggcggggct
     2821 gcaaacttta atctttgcat gggtcaacaa gaaaaagagg aggctgcttc cttcgctgga
     2881 aatgcaatca caatgctttt ttcacttggt atgatccttt gtatcataac aagattattt
     2941 ttaaaaccaa tgatgatctt atttggcgca acatctagtg ttttagatta ttctttaatc
     3001 tatacaggta ttacatctct tggatttcca tttttgatct taacaacagg tggtacgaac
     3061 cttgtccgtg ctgacggaag tccaaagttt tccatgacct gcactttaac tggtgcgatc
     3121 atcaatacga ttcttgatcc aaccttgatc tttggtttcc acatgggaat tgctggtgct
     3181 gcttgggcaa ccgtgatcgg acaggtcgta tctggaatca tggttatttt atatttaaga
     3241 aaatttaaaa cagtaaaact tacaaaagaa aagttaagac cacatcctca ttatatagga
     3301 agaatcgttt ctcttggtat ggcgccattc tttaatcagg tttccatgat ggtcgtacag
     3361 atcgttatga acaatgtttt gatccgttat ggcgcaaaat cttcttacgg aagcgatatt
     3421 ccattggcat gtgccggaat cattacgaag atcaatatga tcttttttgc gattaatatt
     3481 ggtatttctc aaggattaca gccaattatc agttataact atggtgcaag aaagctagat
     3541 cgtgtccgtg aggcttattt gaaagctgcg atttctgcaa cgatcatcag cacagtttct
     3601 ttcatctgct tccagctgtt cccacgacag atcattggaa tctttgggtc tgaaagtgaa
     3661 gcttatttcc gctttgcaga gaagtttttc agaatcttct tgttttgtac ctttattaat
     3721 ggattacagc caattactgc aaacttcttt acagcaattg gaaaggcaaa tcgtggtatt
     3781 ttcctttcat tgaccagaca agttattttc ttgctgcctt tgatgatcat tttgccgatc
     3841 ttctttggaa ttgagggagt tatgttctca gcaccgatcg cagatggatt tgctgcggta
     3901 tgtgcgataa catttgcggt aaaggaattg aagtcgtttc agaaattgtg atttgtagtt
     3961 tgaagacgga aattattggt ggaatattat aaaaaga
//
LOCUS       DAOMUE010000185         3987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6359_length_3987_cov_2.218210, whole genome shotgun sequence.
ACCESSION   DAOMUE010000185 DAOMUE010000000
VERSION     DAOMUE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6359_length_3987_cov_2.218210"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLC71_08095"
     CDS             <1..184
                     /locus_tag="PLC71_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRM02638.1"
                     /translation="IRACIAEVYDEATAEAIRIQYGGSVSAASAPELFAQPDIDGGLV
                     GGASLKPDFGKIVNYK"
     gene            378..1565
                     /locus_tag="PLC71_08100"
     CDS             378..1565
                     /locus_tag="PLC71_08100"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HRM02639.1"
                     /translation="MEKYKRIFVIVLDSLGIGEMPDAARFGDHDVDTFGHICDIVGTL
                     NIPNLKKLGLLNLHPTKEMEEEKHPIAYYTRLKETSNGKDTMTGHWEMMGLKIEKPFL
                     TFTDTGFPPELIHELERCGKKVIGNKCASGTQILDELGEEEIKNGSMIVYTSADSVMQ
                     ICGNEETFDLKNLYRCCEIARELTMKNEWKVGRIIARPYVGKKKGEFVRTSNRRDYAL
                     KPFSRTALNALKDEGFDVISVGKINDIFCGEGITKAYHSESSVHGMQQTVDVCKEDFH
                     GLCFTNLVDFDTLWGHRRNPVGYAKEIEKFDVGLGNLLEVLKEDDLLIITADHGNDPT
                     YTGTDHTREYTPFIAYSKSMKENGRLEDQDTFAVIGATIADNFGVTMPEGTIGISLLS
                     QLK"
     gene            complement(1641..2570)
                     /locus_tag="PLC71_08105"
     CDS             complement(1641..2570)
                     /locus_tag="PLC71_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRM02640.1"
                     /translation="MLHEVSFHSFNGRDEVQGWVYVPACKPKGIVQVIHGFGEHSRRY
                     FHMIVKFMDAGYIVAADDHVGHGKTAMYNDTWGDWGDAGFHTMMEDEHKLKEIVSKMY
                     PDLPYFMFGHSMGSFITRDFAAKYGEELAGITICGTAGHFRGATEAESALLAAVAEGK
                     GKESDPSFTADLMGWMCERCGNISIGNEWICSDPYVQRDHAEDPFDAFTRPTTNQSLL
                     YFVQMMKAITGTEWAGKIPSDLPIYNIGGDQDPVGEYGKGIYEVTNWLVDTGHTVKTR
                     VYSGYRHEIHNYDEIKDEVEAGIISFMDSILDE"
     gene            2788..>3987
                     /gene="gpmI"
                     /locus_tag="PLC71_08110"
     CDS             2788..>3987
                     /gene="gpmI"
                     /locus_tag="PLC71_08110"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRM02641.1"
                     /translation="MSKKPTVLMILDGYGLNENKEANAVAEAKTPVMDKLMKEYPFVK
                     GNASGMAVGLPEGQMGNSEVGHMNMGAGRIVYQELTRITKEIQDGDFFKNEAMLEAIE
                     NCKKNGSALHCFGLLSDGGVHSHNTHLYGVLEMAKRNGLENVYVHLFLDGRDTAPTSG
                     KGFIEELLAKMNEIGVGKVASISGRYYAMDRDNRWDRVQKAYNAIVMGQGNTADSAID
                     AIDASYKEDVTDEFVVPTVIVENGEPVATLKENDSVIFYNFRPDRAREITRSICDDKF
                     DSFDRPNGYFKTTFVCFTEYDVTIPNKLVAFHKVEIKNTFGEFLAQHGLKQLRLAETE
                     KYAHVTFFFNGGVEEPNEGEDRILVKSPAVATYDLQPEMSAPEVGAKLVEAIKSDKYD
                     VIIMNFAN"
BASE COUNT         1439 a          674 c          801 g         1073 t
ORIGIN      
        1 aattcgtgct tgcatcgcag aagtatatga tgaagctaca gcagaagcaa tccgcattca
       61 gtatggtgga tctgtatctg cagccagcgc accagaatta tttgcacagc cagatatcga
      121 cggtggatta gttggtggag catcattaaa accagacttt ggtaaaatcg taaactataa
      181 ataaatttga ttaattaaat gatgggtccc tcggatattg aatatccggg ggattttttc
      241 ttttttatat aatggtagaa ttcgttaaaa gattaaagaa ataaaagact agaagcaagc
      301 aataaatcgg tttataataa ataataagat caagtacaaa aaagattccg agagtagatg
      361 aaaagggggc atatgatatg gagaaataca aaagaatttt tgtcattgtc ttagattcct
      421 taggaatcgg agaaatgcca gatgcagcca gatttggaga tcatgatgta gacacctttg
      481 ggcatatctg tgatatcgtg ggaacattaa atattccaaa tctaaaaaag cttggattgt
      541 taaatttgca tccaacaaaa gaaatggaag aagaaaaaca tccaattgcc tactatacca
      601 gattaaaaga aactagtaac ggaaaagaca ctatgacagg tcactgggag atgatgggac
      661 ttaagatcga gaaaccattt cttaccttta cagacacagg ttttccacca gaattgatcc
      721 atgaattaga aagatgtgga aagaaagtta ttggcaataa atgtgccagt ggaacgcaga
      781 ttttggatga attaggtgaa gaagagatta aaaatggttc aatgatcgta tatacatctg
      841 ccgattctgt gatgcagatc tgtggaaatg aagaaacttt tgatcttaag aatctttatc
      901 gctgttgtga gatcgcaaga gagcttacga tgaaaaatga atggaaagtc ggaagaatca
      961 ttgcaagacc atatgtggga aagaaaaaag gcgaatttgt aagaacaagc aatcgaaggg
     1021 attatgcttt aaaaccattt tcaagaactg ctttaaatgc attaaaagat gaaggatttg
     1081 acgtaatttc agtaggaaag atcaatgata ttttctgtgg agaaggaatc acaaaagcat
     1141 atcattctga aagctctgtg catggaatgc agcagaccgt ggatgtatgc aaagaagatt
     1201 ttcatggact ttgttttacc aatctggtag actttgatac actctgggga catcgaagaa
     1261 atccagtcgg ctatgcaaaa gagatcgaga aatttgatgt tggtttagga aatctgttag
     1321 aagttttaaa agaagatgat ttattgatca tcaccgcaga tcatggaaat gatccaactt
     1381 atacaggaac agaccataca agagaataca caccatttat cgcatattca aagtctatga
     1441 aagaaaatgg aaggctagaa gatcaagata catttgcagt gatcggagct acgatcgcag
     1501 ataattttgg tgttacgatg ccagaaggaa cgatcggaat atctttatta agtcaattaa
     1561 aatagtacaa ctgaaataaa aacaccacag aaggaaataa agatctcctg tggtgttttg
     1621 tttataattt ggtatgtata ttattcatct aaaatcgaat ccataaaaga aataatacca
     1681 gcttccacct catctttgat ttcatcataa ttatgaatct catggcggta accagaataa
     1741 acacgagttt ttactgtatg tccagtatct acaagccagt tggtaacttc ataaatacct
     1801 tttccatatt caccaacagg atcctgatct ccaccgatat tataaatcgg aagatcagaa
     1861 ggaatcttgc cagcccattc ggtaccagtg atcgctttca tcatctggac aaaataaagc
     1921 agagattgat ttgtagttgg ccgggtaaat gcatcaaatg gatcttctgc atgatctcgc
     1981 tgaacataag gatcagaaca gatccattca ttgcctatgg aaatattacc acaacgttca
     2041 cacatccaac ccattagatc agcagtaaag gaagggtcag attctttccc ttttccttca
     2101 gctacagcag ccaataaggc agattcagct tctgtagcac cacggaaatg accagctgta
     2161 ccacagattg tgattcctgc aagttcttct ccatattttg cagcaaaatc tctcgtaata
     2221 aaagatccca tgctgtgtcc aaacataaaa taaggaagat caggatacat tttgctgacg
     2281 atttctttta acttatgttc atcttccatc atcgtatgga aaccagcatc accccagtca
     2341 ccccaggtat cgttatacat tgcagttttt ccatgtccaa catggtcatc tgcagcaacg
     2401 atataccctg catccataaa ttttacgatc atatggaaat aacgacggga atgttcacca
     2461 aagccatgga tcacctgtac aattcctttt ggtttgcagg caggtacata aacccaacct
     2521 tgcacctcat cacggccatt aaaggaatga aaactaactt catgaagcat actaagcacc
     2581 tccaaatctg taaaaactaa tatgcaagca aagaaaagac gaatcaacat attagttata
     2641 taaaattgta tctgaatata ttatactcca gattaaaatg tacgtccata aatacaatca
     2701 tataatagtt gaatttcaag aagaaaatcg ttataatatt aacggacata aaataagatt
     2761 aatttttggg aaatgaagga gaaaataatg agtaaaaaac caacagtcct gatgatttta
     2821 gatggatatg gattaaatga aaacaaagaa gccaatgcag tagcagaagc aaagactcct
     2881 gtaatggata aattaatgaa agaataccca ttcgtaaaag gaaatgcatc tggaatggca
     2941 gtaggacttc cagaaggtca gatgggtaac tctgaagtag ggcatatgaa tatgggtgca
     3001 ggacgcatcg tatatcagga attaacaaga atcacaaaag agatccagga tggagatttc
     3061 tttaagaacg aagcaatgtt agaagcaatt gaaaactgca agaagaatgg ttctgcatta
     3121 cattgttttg gattattatc agatggtgga gtacacagcc acaatacaca tttatatggc
     3181 gttcttgaaa tggctaagag aaatggtcta gagaatgtat atgttcattt attcttagat
     3241 ggacgtgaca cagctccaac atcaggaaaa ggattcatcg aagaattatt agcgaagatg
     3301 aatgagatcg gagtaggtaa agtggcatct atctctggac gttactatgc aatggatcgt
     3361 gacaatcgtt gggatcgtgt acagaaagca tacaatgcga tcgttatggg acagggaaat
     3421 acagctgaca gtgcgatcga tgcaattgat gcttcttaca aagaagatgt tacagacgaa
     3481 ttcgtggtac caacagttat tgttgaaaat ggtgaaccag ttgcaacatt aaaagaaaac
     3541 gattccgtta ttttctataa cttcagacca gaccgtgcaa gagagatcac acgttctatc
     3601 tgtgatgaca aattcgacag ttttgatcgt ccaaatggat acttcaagac aacatttgta
     3661 tgcttcacag aatatgatgt gacaatccct aacaaattag tggcattcca caaagtagag
     3721 atcaagaata catttggaga attccttgca cagcacggat taaaacagtt acgtttagca
     3781 gaaacagaga aatacgctca tgtaacattc ttctttaacg gtggagtcga agaaccaaac
     3841 gaaggagaag acagaatctt agtaaaatct ccagcagtag caacatatga tcttcagcca
     3901 gagatgagtg ctccagaagt tggtgcaaaa cttgtagaag caatcaaatc tgataaatat
     3961 gacgtgatca tcatgaactt tgcaaat
//
LOCUS       DAOMUE010000186         3985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6360_length_3985_cov_2.567430, whole genome shotgun sequence.
ACCESSION   DAOMUE010000186 DAOMUE010000000
VERSION     DAOMUE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6360_length_3985_cov_2.567430"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..988
                     /locus_tag="PLC71_08115"
     CDS             191..988
                     /locus_tag="PLC71_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM02642.1"
                     /translation="MKKYKGVVFFDYDGTTVDEVDEIKTATPTTVESLNKLKENGYLT
                     MLCSGRTQRFLEMDIDKFMGAITCNGSHTEVEGEVIRDICIPDELVFQVVNEYFPRDT
                     IIHFETRDISYYLHMDEEFFKNHCKLFNLPQRWYAPWKFRTKNTHISKLVMNYKDIQV
                     KKDFEEEFKDVFTCAKHVRENFIDITLKGVTKGDAITELIEKLGIDKKDTYAFGDADN
                     DVEMMQAVGTGIAMGRHSEKVGEVASMVTGTVKEEGITMALKKLGLI"
     gene            990..1685
                     /locus_tag="PLC71_08120"
     CDS             990..1685
                     /locus_tag="PLC71_08120"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM02643.1"
                     /translation="MKNIIFDVDDTLYNLMEPFEKAHKELMAARTDADCEELFEASRT
                     YSDEAFCMSREGKISEDEEFAYRVQKTYADVGVEVSKEEAKQFEERYRYYQKHIQVPE
                     ITKQILDHCKENYRIGILTNGTTKNQGKKLETLGLDHWFNPKTMFISDSIGAAKPDVK
                     AFHTVQKEMKLDPEETWFIGDTFEIDVVGAKNSGWHVIWFNHRNRPMPEGDIVPDVEV
                     TSGQELFEYIKKL"
     gene            complement(1828..>1907)
                     /locus_tag="PLC71_08125"
     CDS             complement(1828..>1907)
                     /locus_tag="PLC71_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRM02644.1"
                     /translation="EEGQSVSFEIEEGPRGKQASNVTKL"
     assembly_gap    1908..1917
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1918..2039)
                     /locus_tag="PLC71_08130"
     CDS             complement(<1918..2039)
                     /locus_tag="PLC71_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRM02645.1"
                     /translation="MSNGTVKWFNAEKGYGFISQESGDDVFVHFSAIQGKGFKT"
     gene            complement(2243..2668)
                     /locus_tag="PLC71_08135"
     CDS             complement(2243..2668)
                     /locus_tag="PLC71_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HRM02646.1"
                     /translation="MDNENLLNSIYQNIAMVLETIPEIRKITTDCDFKALIISQEEEY
                     QNLSDSIKDTITSNGDSTEELNDLLKTYSEWMTKLKAMSDKNVSHLSEMCIQGYTMGM
                     IKAIQKNHLYCDASKEAKHFGDKLISLHEKSIQDLKKYL"
     gene            2814..3272
                     /locus_tag="PLC71_08140"
     CDS             2814..3272
                     /locus_tag="PLC71_08140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HRM02647.1"
                     /translation="MSKENFEKQIRKRMEQLDEINKHGIFKELKGTVTDFNYDTKSLT
                     MTFEATKFHENAFGIMFGGSLAGMFDIAFGTLTAGLGDYSVAPTVQLSTTFLKGIPTG
                     ATVRTEVEAVSTGKTIMNFVGKAYVGEELVGSASGTFMTPRPFKKFNTEK"
     gene            complement(3354..>3985)
                     /locus_tag="PLC71_08145"
     CDS             complement(3354..>3985)
                     /locus_tag="PLC71_08145"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRM02648.1"
                     /translation="IFINTQVIANAPEKYLWAGIGDTMAKFYECTTSARCDNDLDHST
                     SMGVQISNLCARPLVKYGVEALEECRNHTPGKALEEVILGIIVSTGFVSNLVGIDLNT
                     GLAHACYNGFTVCKSTEEHGHLHGEIVAYCILILLKVDHQEEEFKKIYEFSKNMGFPV
                     KLADIHATIDDMDAVITKALSGIDVRVWPYEVTPDMILDAVKEIEQVAL"
BASE COUNT         1442 a          650 c          737 g         1146 t
ORIGIN      
        1 gtgaaaaaga tacttttatc aagagaagat agaagtgtct ttttctgatt tatgcatttt
       61 tttcaaacat acacacacct ggtatttttt attggtaaaa tcttgaaatt aaaagctgta
      121 gaaagtaaaa taaagtgtgt attatatata gagaaaataa gaaatgaata aaatgggaga
      181 aagcaaaatt atgaaaaagt acaaaggtgt cgtatttttt gactatgatg gaacaacagt
      241 tgatgaagta gatgagatta aaacagcaac accaacaact gtagaatcat tgaataaatt
      301 aaaagaaaat ggatatctga caatgttatg ctcaggaaga acacagcgat ttttagagat
      361 ggatattgat aaatttatgg gagcgatcac atgtaatggt tcacatacag aagttgaggg
      421 agaagtgatc agagacatct gtattccaga tgaattggta tttcaggtag ttaacgaata
      481 tttcccaaga gatacgatca ttcattttga gacaagagat atcagttatt atctacatat
      541 ggatgaagag tttttcaaaa atcactgcaa actttttaat ctgccacaac gatggtatgc
      601 gccatggaaa ttccgcacaa aaaacactca catcagcaaa cttgttatga attacaagga
      661 tattcaggtg aagaaggatt ttgaagaaga atttaaagat gtatttactt gcgctaaaca
      721 tgttcgcgaa aatttcattg atatcacatt aaaaggtgta acaaaaggag atgcgatcac
      781 agaactgatc gagaaacttg gaattgacaa gaaagacaca tatgcatttg gagatgcaga
      841 taacgatgta gaaatgatgc aggcagttgg aacaggaatc gcgatgggac gtcatagtga
      901 gaaagtagga gaagtcgcat ctatggtgac aggaactgtc aaagaagaag gaattacaat
      961 ggcacttaag aagttaggat tgatctaaga tgaagaatat tatttttgat gtagatgata
     1021 cattatataa tttaatggaa ccatttgaaa aagcccacaa agaactgatg gcagcccgca
     1081 cagatgcaga ctgtgaggaa ttatttgaag catcaagaac ttacagtgat gaagcattct
     1141 gtatgtcaag agaaggaaaa atctctgaag acgaagaatt tgcatatcgt gtccagaaaa
     1201 catatgcaga tgttggagta gaagtcagca aagaagaggc aaaacaattc gaagaacgat
     1261 accgttatta ccaaaagcat attcaggtac cagaaattac aaaacagatc cttgatcatt
     1321 gcaaagaaaa ttacagaatt ggaattttaa cgaatggaac aacaaagaat cagggaaaga
     1381 aattagagac attaggatta gaccactggt tcaaccctaa aacaatgttc atctcagaca
     1441 gcatcggagc tgcaaaacca gatgttaaag cattccatac tgttcaaaaa gaaatgaagc
     1501 ttgatcctga ggaaacatgg tttatcggag acacctttga aatagatgtc gtaggagcca
     1561 agaattctgg ctggcatgtc atctggttca accacagaaa ccgcccaatg ccagaaggag
     1621 atatcgttcc ggacgtagaa gtaacaagtg gacaggaatt atttgaatat ataaaaaaat
     1681 tataatttaa caccgtctaa catacacaca tgtggtggtt tgaatagctg tccatgtata
     1741 tgagaaaaac gaaataatag gataaaagat ttagaacttt tatcctatta tttcgttttt
     1801 ttatatgcat tgaacaacgc attcaaatta caacttggtt acattagatg cctgttttcc
     1861 gcgaggtcct tcttcaatct caaaactaac actctgtcct tcttccannn nnnnnnnaaa
     1921 gtcttaaatc cttttccctg aattgcagaa aaatgaacaa atacatcatc tccggattcc
     1981 tgtgaaataa atccataacc tttttctgcg ttaaaccatt ttactgtacc attactcatc
     2041 tgggtacctc tactttctta aataaaaaat acatgtaact gttaatgaca ggtgtttcaa
     2101 taaaaatctt tataaaaaag aaaaagtcca cgaaccgtta cttgctttat gcattcatag
     2161 aaataaatga atgcatccgt atcggtcgtg aacattaaaa tctatcaata acaagattaa
     2221 tcatagcaca acaacttaac gattacaaat acttttttaa atcctgaata cttttttcgt
     2281 ggagagaaat aagtttgtca ccgaaatgtt ttgcttcttt ggaggcatca cagtaaagat
     2341 gatttttctg gattgctttg atcattccca tcgtatatcc ctgaatacac atttcagaaa
     2401 gatgactgac atttttatca gacatagcct ttaatttggt catccattca gaatatgttt
     2461 tcaaaagatc atttaattct tctgtagaat caccattgga agtgatcgta tcttttatag
     2521 aatctgataa attttgatat tcttcttcct ggctgatgat caaagcctta aaatcacagt
     2581 ctgtcgtgat ctttcgaatt tcaggaatgg tttctaaaac cattgcaatg ttctgataaa
     2641 tactgtttaa taaattttca ttatccataa ttgtaaaact ccttttgttc ataggatgtt
     2701 ccatataaca gtttctatac ataaaagtgg aaatatgata aaatggcaga cataaaatga
     2761 aatctacata catcgattta atggtgaaaa gaaataataa gcaggagaaa aagatgagca
     2821 aagaaaattt tgagaagcag atccgaaaaa gaatggagca gttagatgaa atcaataaac
     2881 atggaatatt taaagaatta aaaggaactg tcacagattt taattatgat accaaaagtt
     2941 taaccatgac gttcgaggcc acaaagtttc atgaaaatgc atttggaatc atgtttggag
     3001 gttctctggc agggatgttt gatattgcat ttggaacatt gactgctgga ttgggcgatt
     3061 atagtgtagc cccaacggtg cagctttcta caacattttt aaaaggaata ccaacaggag
     3121 caaccgtaag aacagaggta gaagcagtgt ccacaggaaa aacgatcatg aattttgttg
     3181 gaaaagccta tgtaggagaa gaactcgtcg gaagcgccag cgggacattt atgacaccgc
     3241 ggccatttaa gaaatttaat acagaaaaat aaagaaaaag gatattgatc gtgacgccaa
     3301 tatccttttt atatcatagg aaattccaag ggaatccact tgataaagtt attttataaa
     3361 gcaacttgtt caatctcttt tacagcatca aggatcatat caggagttac ttcataaggc
     3421 catacacgta catcaattcc ggataaagcc tttgtgatca cagcatccat atcatcaatc
     3481 gtcgcatgaa tatccgcaag tttcacagga aatcccatat tcttggagaa ttcatagatt
     3541 ttcttaaatt cctcttcctg atgatcaact tttaataaga tcaggataca ataagcaacg
     3601 atctcaccat gcagatgtcc atgttcttcg gtagatttac atacagtaaa tccgttatag
     3661 caggcatgtg caagtccggt atttaaatca attccgacaa gatttgatac aaaaccagta
     3721 gaaacgataa ttccaagaat gacttcttcc agagcttttc caggagtatg atttcgacat
     3781 tcttctaagg cttctacacc gtattttact aatggtctgg cacaaagatt actgatctgg
     3841 acgcccatgg aagtgctatg gtctagatca ttatcacaac gggcactggt tgtacattca
     3901 taaaatttag ccattgtatc accaattcca gcccacaggt acttttcagg agcattggcg
     3961 atcacttgtg tattgataaa gatcc
//
LOCUS       DAOMUE010000187         3963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6405_length_3963_cov_2.822671, whole genome shotgun sequence.
ACCESSION   DAOMUE010000187 DAOMUE010000000
VERSION     DAOMUE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6405_length_3963_cov_2.822671"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLC71_08150"
     CDS             complement(<1..325)
                     /locus_tag="PLC71_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HRM02649.1"
                     /translation="MKKKDIIEGKIIKTEFPNKGTFICEDQKVTVKGVIDGQTIKGQV
                     TKKRKSGCVVRLLDVLEKSPLEDAKPVCPHFGICGGCFYQTVSYENQLKIKEGMVRDL
                     LKDYVN"
     gene            complement(410..916)
                     /locus_tag="PLC71_08155"
     CDS             complement(410..916)
                     /locus_tag="PLC71_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM02650.1"
                     /translation="MRILVISDSHGRNDDIEGVLKQVGDIDMMIHCGDVERGDSYIRE
                     IADCPVVMVAGNNDYYLDLPSEEEVRIGDYKVLVTHGHGYYVNSGVDYLREHALEYGY
                     DVVMYGHTHVPYIEIGDDVTILNPGSISYPRQPGRKPTFLIMEIDEEGQAHYAHGYYK
                     EQFDELML"
     gene            complement(937..1521)
                     /locus_tag="PLC71_08160"
     CDS             complement(937..1521)
                     /locus_tag="PLC71_08160"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HRM02651.1"
                     /translation="MKQRLIFATGNEHKMKEIREILDESKYEIISMKEAGVDIDIVED
                     GKTFEENALIKAKAVMEVTGQLTLADDSGLEIDALNGEPGIYSSRYLGEDTSYVKKNS
                     VILERLKDVPEEKRSARFVCAVAAAFPDGQTKVIRGTMEGIIGYEIKGENGFGYDPIF
                     YLPQYGKYSAELSSDEKNAISHRGEALRRIREVL"
     gene            complement(1521..2666)
                     /locus_tag="PLC71_08165"
     CDS             complement(1521..2666)
                     /locus_tag="PLC71_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRM02652.1"
                     /translation="MKTVELIAPCHFGLEAVLKREILDLGYEIVKVEDGRITFRTDLD
                     GIARANIFLRTAERILLKIGSFKAYTFDDLFEGTKKLPWEDYIPENGRFWVKKASTAK
                     SKLFSAPDIQSIVKKAMVDRMKSKYKVSWFNEDGDDYPVRVFILKDQVTISLDTTGIP
                     LHKRGYRKLVSEAPIRETLAAALLMLTPWHKDRILVDPFCGSGTFLIEAAMMGMNMAP
                     GMNRSFESEHWSNFLPKKAWYDTIDEANDLVNHDIEMDLQGYDKDPRMLKIARQNAQD
                     AEVDHLIHFQQREVKDLRHPKPYGFIIANPPYGERLEDQETLPQLYTEMKEAFDRLDT
                     WSKYVITSYEDTEKYLGKPTKKRKVYNGMIRGEFYQYLGPKPPRRRK"
     gene            complement(2748..3065)
                     /locus_tag="PLC71_08170"
     CDS             complement(2748..3065)
                     /locus_tag="PLC71_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRM02653.1"
                     /translation="MEFENISLKEGIKLSRDKKHYILMDVREEERYKEGHLENAISCP
                     YGILKECYEQMDGKKIIVYCDFGGQSMMAARHLRKDGFEVYNIIGGVYYYMKEKEQNK
                     SEA"
     gene            complement(3179..3328)
                     /locus_tag="PLC71_08175"
     CDS             complement(3179..3328)
                     /locus_tag="PLC71_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02654.1"
                     /translation="MAKNCSNSSNKNQSSNTSNSGGSNRSKNQNKNKSQNSSTNSNKN
                     ANDEY"
BASE COUNT         1054 a          895 c          563 g         1451 t
ORIGIN      
        1 cattgacata atcttttaac agatcacgta ccattccttc tttgatcttt aactggtttt
       61 cataagatac cgtctgataa aaacatccac cgcatattcc aaagtgtgga cagactggtt
      121 ttgcatcttc taatggagat ttctctaata catctaacaa tcttaccaca cagccacttt
      181 ttctcttctt ggtaacctgc cctttgatcg tctgcccatc aatgacacct ttgactgtaa
      241 ctttctgatc ttcgcagata aatgttccct tattcgggaa ttctgtcttt atgatctttc
      301 cttcaatgat atcttttttc ttcattgtca tccctttctt atcttatcaa atcaaaccta
      361 tcgcagaaaa aagatggaaa taccacctac gatacttcca tcattcatat tatagcatta
      421 attcgtcaaa ctgttctttg taatatccat gggcataatg tgcctgtcct tcttcgtcaa
      481 tctccataat aagaaatgtt ggcttccttc ctggctgacg aggataagat atacttcctg
      541 gattcaagat cgttacatca tccccaatct caatatatgg cacatgagta tgtccataca
      601 ttacaacatc atatccatat tccaaagcat gttctcttaa ataatccaca cctgagttta
      661 cataatagcc atgtccatga gtcactaata ctttataatc gccaattctc acttcttctt
      721 cgcttggcag atctaagtag taatcattgt tgcctgcgac cataactact gggcagtccg
      781 ctatttctcg tatgtatgaa tctccacgtt ccacatctcc gcaatggatc atcatatcga
      841 tgtcgcctac ctgttttaaa actccctcga tatcatcatt gcgtccatgg gaatcactga
      901 tcaccaatat cctcataaac tttctccttg ttttatttac aaaacttctc gtatacgtct
      961 cagtgcttcg cctctgtgac tgatcgcatt cttctcatca ctgctcagtt cggcactata
     1021 cttcccatat tgtggtaaat agaagatcgg atcatatcca aatccattct ctcctttgat
     1081 ctcatatcct ataattcctt ccatagtacc acgaattacc ttcgtttgtc catctggaaa
     1141 tgcagctgcg accgcacaaa cgaatctcgc gcttctcttc tcttctggta catcttttaa
     1201 tctttcaagg atcacactgt ttttcttcac ataagaagta tcttctccaa gatatctgga
     1261 agaatagatt ccaggttctc catttagtgc atcaatttct aatcctgaat catctgcaag
     1321 tgttaactgt cctgtgactt ccatcactgc ttttgctttg atcaacgcat tttcttcaaa
     1381 tgtttttcca tcttctacaa tgtcaatgtc tactcctgct tctttcatag aaatgatctc
     1441 gtatttggat tcatcaagga tctctctgat ctctttcatc ttatgttcgt tgcccgtggc
     1501 aaagattaat ctctgtttca ttattttctc cttcttggcg gtttcggtcc aaggtactgg
     1561 taaaactctc ctcggatcat tccattgtaa actttacgtt tctttgttgg ttttccaaga
     1621 tatttttctg tatcctcata agaagtgatg acatacttag accatgtatc caagcggtca
     1681 aatgcttctt tcatctctgt atatagctgt gggagagtct cctgatcttc cagtcgttct
     1741 ccatatggag ggttagcaat gataaatcca tatggctttg gatgtcgtaa atctttgacc
     1801 tctctttgct gaaaatgaat caagtgatca acttctgcat cctgagcatt ctgtcttgcg
     1861 atctttaaca ttcttggatc tttgtcatat ccctgaagat ccatctctat atcatgattg
     1921 accagatcat ttgcctcatc aatggtatca taccatgctt tctttggcag gaaattgctc
     1981 caatgttcag attcaaaaga acggttcatt cctggtgcca tattcatccc catcattgcc
     2041 gcttcgatca aaaatgttcc acttccgcag aatggatcga ctaagattcg atccttatgc
     2101 catggtgtga gcataagaag agctgctgcc agtgtctcac ggattggtgc ttcactgacc
     2161 aacttacggt atcctctctt atgaagcggt atacctgttg tatctaaact aatcgtcacc
     2221 tgatccttta aaataaatac gcggaccgga taatcatctc catcttcatt aaaccatgat
     2281 actttgtatt tagacttcat acgatcaacc atagccttct tgacaattga ctggatatcc
     2341 ggtgcgctaa acaatttact cttcgctgtt gatgccttct ttacccagaa acgtccattc
     2401 tctggaatat aatcttccca tggaagtttc tttgttcctt caaacagatc atcaaatgta
     2461 tatgctttaa aagaacctat ttttaataag attctttctg ctgttcttaa aaatatattc
     2521 gcacgggcaa tcccatctaa atctgtacga aatgttattc gcccgtcttc tactttaaca
     2581 atttcatatc ctaagtccag gatctctctt tttaatactg cttccagccc aaaatggcat
     2641 ggtgcaatca gttcaactgt cttcactcaa ttctcctttg tatttttatt ctgttcctat
     2701 tctaccgttt tccttatttt tcgtcaactg tcaacttttg taacatgcta agcctctgac
     2761 ttattttgtt ctttttcttt catataataa tagacaccac cgatgatatt ataaacttca
     2821 aatccatctt tcctcaagtg tctggctgcc atcatactct gtccgccgaa atcacaataa
     2881 acaatgatct ttttcccatc catctgttca taacactctt ttaagattcc atagggacag
     2941 ctgatcgcat tttctaaatg cccttctttg taccgttctt cctctctgac atccatcaaa
     3001 atataatgct ttttatctct tgaaagcttg atcccttctt ttaatgatat attttcaaat
     3061 tccataaaag tcctcttttt gttagcatat gcacatcaca gactcttgga actaacaagt
     3121 attttctata gaaaagagga atccttatgc tgatttcaca aggattcctc tcttctaatt
     3181 agtactcatc atttgcattt ttgttgctgt ttgttgaact attctgactt ttgtttttat
     3241 tctggttctt tgatctgtta gatcctccgc tgttgcttgt attggaagac tgatttttgt
     3301 tacttgaatt cgaacaattc tttgccatgt tcattctcct ttctttttag tacagcatta
     3361 ttttgtgcga aatctctttc atatatacac aaaaaaaatt attttacttt tattttactt
     3421 tttttaaaaa aatcacttga tttttttcaa ccaatcatat atcatgaact tatcgaggat
     3481 atgtttattt tcgattataa ccccttatta atttatttat actcccctca aaaagagcag
     3541 atttccctat ctgctctttt tactatttat ctttaatgtt tttaaaaatg tacaaatgat
     3601 tttattgaca ttttctatag tatgtgtata attaagattg ttcaagaaca aaggcgttcc
     3661 gatcatagga ttggagcgcc tttttttgta tatcaatggt aattccttgg gtcttctcca
     3721 tcgatatacg aaattctttc ttaagacttt atacataacc gcgtactagt ttttgacaag
     3781 tttttggcat cttgcagaca agagtaacgg acacatcatt gacttgaaca cagacttgct
     3841 atgttgacta gaatgagact aacaatgacg actagaaatg agactagaat gagactgaca
     3901 atgacaacta gaacgagact agaatgagac gaaatactat gactagtttt gaagcttaaa
     3961 agt
//
LOCUS       DAOMUE010000188         3927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6489_length_3927_cov_2.569990, whole genome shotgun sequence.
ACCESSION   DAOMUE010000188 DAOMUE010000000
VERSION     DAOMUE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6489_length_3927_cov_2.569990"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PLC71_08180"
     CDS             complement(<1..1088)
                     /locus_tag="PLC71_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRM02655.1"
                     /translation="MNTLTHVGNLFAFIGGLGMFLYGMDIMASGLQKTAGNKMKDLLG
                     YLTNNRILGVLVGALVTAIIQSSSATTVMVVGFVNAGIMNLTQAAGVIMGANVGTTIT
                     AWLVSANEWAGALKPDFFAPFILGIGAFIITFSNKQKMTQKAEIAVGFGILFIGLSSM
                     SSSIGVYQDSPVFYKAFQVLGGNPILGILVGAVVTAIIQSSSASVGILQTLAMRGIVN
                     WRSAIFITLGQNIGTCITAILSSAGANKTAKRAAVIHLSFNVLGAAIYGVIMTIFFGI
                     FPDLAMQHISSTQISIFHSIFNISVTILLFPFANALVKLSGLIIREEPVEDEEEEPEE
                     KALRHLDPRILETPAFAVENAVKEVVRMG"
     gene            1296..2105
                     /locus_tag="PLC71_08185"
     CDS             1296..2105
                     /locus_tag="PLC71_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRM02656.1"
                     /translation="MILADYHLHTSFSSDSESPMEEMIRHAISLGLKTICFTEHHDID
                     YPVNPDGFDFLLDLPSYKEEIFKMKEHYQDQIEINYGVELGLMPTTLDQCAAFVKDEP
                     FDFIIGSTHIVDFMDPYDAIYFETYPARLGLAHYFEDILKNISSFNHYCVYGHLDYAA
                     RYIKDPSFEFHYRDYSDLLDAILRMIIENGKGIEVNTAGLKYGMSDPNPHHEIIKRYM
                     ELGGEIITIGSDGHCPKHMAYDFYKLPDFLSNLGIRYYTVFRDKKPEFIKL"
     gene            complement(2181..3050)
                     /locus_tag="PLC71_08190"
     CDS             complement(2181..3050)
                     /locus_tag="PLC71_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM02657.1"
                     /translation="MRYEGMIYRPPSEAYSLIVQVTIGCSQNRCIFCSMFKEKRFRIR
                     KVEEVLEDLQDARNKYRYVEKIFLADGDALICKMTDLEAILHFIKDQFPECKQVTLYG
                     SPRSILMKQQEDLDKLRELGISMIYMGLESGNDEVLTYMKKGVTSQEMIEAAQKVKQA
                     KIRLSVTAISGLGGRRLWKEHAKDTGLVLSQMKPDYIGLLTLMIEEDLPLADKIRSGE
                     FEMLNPYDILIETKEMLKYMDCPDCIFRSNHASNYVNLRGTLNQDKEAMINLLDEAIK
                     GNVHLKSEWMRGF"
     gene            complement(3071..3748)
                     /locus_tag="PLC71_08195"
     CDS             complement(3071..3748)
                     /locus_tag="PLC71_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRM02658.1"
                     /translation="MIKTIKKVAVIIITVVIMISTVTIADQASSTTVQAASGYSGKIY
                     KHWCKLRTAKSKRSKTIKKLSVGTKVTVLSTSGSWRKIKVGNKTGYALKKYVYISTNA
                     PKLTGSTYNKGQTVASFAQRFVGNPYVWGGTNLNTGADCSGFVKSVYKSFGYNLPRSS
                     SSQRSAGRKVSYSNKQPGDLICYSGHIAIYIGNGKIVHASSRKTGIKISPKANYRKVL
                     SVRRIVK"
BASE COUNT         1121 a          794 c          577 g         1435 t
ORIGIN      
        1 cccatacgta ccacttcttt cactgcattc tcaactgcaa atgcaggtgt ttcaagaata
       61 cgtggatcta agtgacgtaa tgctttctct tctggttctt cttcttcgtc ttctactggc
      121 tcttcacgaa tgataagtcc agataatttt accaatgcat tcgcgaatgg gaataacagt
      181 atcgtcacag atatgttaaa gatcgagtgg aaaatggaaa tctgtgtact ggatatatgc
      241 tgcattgcaa gatctgggaa gattccaaag aaaatcgtca tgatcacccc ataaatagct
      301 gcacctaata cattaaaact taaatggatc acagctgctc tttttgctgt tttatttgca
      361 cccgcactag ataagattgc tgtgatacaa gtaccaatat tctgccctaa cgtaataaag
      421 atcgctgatc tccagtttac gattcctctc atcgctaatg tctgtaaaat accgacagat
      481 gcagatgaac tctgaatgat cgctgtaaca acagcgccga ctaaaatacc aaggatcgga
      541 tttcctccta atacctgaaa tgctttgtag aataccggac tatcctgata tactccaatg
      601 gatgatgaca tagatgataa tccaataaat aaaataccaa atccgacggc aatctccgct
      661 ttctgtgtca tcttctgctt attagagaat gtaatgatga aagctccgat tcctaaaata
      721 aatggtgcaa aaaaatctgg ttttaacgct cccgcccatt cattagctga taccagccat
      781 gccgtgatcg ttgtacctac attggctccc atgataacac cggctgcctg tgtaagattc
      841 ataatccctg catttacaaa acctacaacc ataacagtgg ttgctgatga actctgaata
      901 attgctgtaa ccagtgctcc tacaagtaca cctaaaatac gattatttgt taaatagcca
      961 agcaggtctt tcatcttatt tcctgctgtc ttttgaagtc ctgatgccat gatatccatt
     1021 ccatacagga acattccaag tccgccgata aacgcaaaca gattaccgac atgtgttagt
     1081 gtattcattt tttacgattc ctcctgtgtt tttacagacc ctcttattgg atctcccaca
     1141 tatatttcat ttgtcgttat tccgacataa ataattttaa caaacctact cttatattta
     1201 aagaactttt tacatagatt ttattttttc tttacctttc tgtcataatg tatataataa
     1261 aagtattata ttaactattt acaaaggagt tttttatgat tcttgcagat tatcatttac
     1321 ataccagttt ttcttccgat tctgaatctc ctatggaaga aatgatccga catgctattt
     1381 cacttggtct taaaactatt tgttttacag agcatcacga tattgattat cctgttaatc
     1441 ctgatggatt tgatttcctt ctagatcttc cttcctataa agaagaaata tttaaaatga
     1501 aagaacacta tcaggatcag attgaaatca attatggagt ggaacttgga ttaatgccaa
     1561 caaccctaga tcaatgtgct gcattcgtca aggatgagcc ttttgatttt atcattggtt
     1621 ctactcatat tgttgatttt atggatccat atgatgcgat ttattttgaa acatatcctg
     1681 cccgtctggg acttgctcat tattttgaag atatcttaaa aaatatttct tcttttaatc
     1741 attattgtgt ctatggacat cttgattatg ctgcacgtta tatcaaggat ccttcttttg
     1801 aattccatta tagagattat tctgatcttt tagatgccat tttaaggatg atcattgaaa
     1861 atggcaaagg tattgaagtg aatactgctg gacttaaata cggaatgagt gatccaaatc
     1921 ctcatcatga gatcataaaa agatatatgg aacttggtgg tgaaattatt acgatcggat
     1981 ctgatggcca ttgtccaaaa catatggctt atgattttta taaattacct gattttctgt
     2041 ctaatcttgg aattcgttat tataccgtct ttcgagataa gaaacctgaa ttcattaagt
     2101 tatgagtttc tctgcataaa aaggaacgag acaataggct cataatattc ctattgtctc
     2161 gttccttgat tctagctata ttagaatccc ctcatccact ctgatttcaa atgaacattt
     2221 cctttgattg cctcgtctaa cagattgatc attgcttcct tatcctgatt taatgttcct
     2281 cttaaattca cataattaga tgcatgattc gatctgaaaa tacaatctgg gcaatccata
     2341 tattttaaca tttcttttgt ctcgatcaaa atatcatatg gatttaacat ttcaaattct
     2401 ccgcttctga tcttatctgc taacggaaga tcttcttcta tcattaatgt aagcaatcct
     2461 atataatctg gcttcatctg ggataaaaca agtcctgtat cttttgcatg ttccttccat
     2521 agccgtcttc cacctaaccc tgatatagct gtcacagata aacggatctt tgcctgtttt
     2581 actttctgtg ctgcctctat catctcttgt gatgttacac cttttttcat atatgtaagg
     2641 acttcatcat ttcctgattc caatcccata tagatcatag atattccaag ttctcttaat
     2701 ttgtccaaat cttcttgttg tttcatcaaa atgcttcttg gcgatccgta taaagttact
     2761 tgtttacatt ctgggaattg atctttgata aaatgaagga ttgcttcaag atctgtcatc
     2821 ttacaaatca atgcatctcc atctgccaaa aaaatcttct ctacatatcg atacttattt
     2881 cttgcatctt gtaaatcttc taagacttct tctacttttc tgatcctaaa tcgtttttct
     2941 ttaaacatgc tgcaaaaaat acaacgattc tgactacatc cgatcgttac ctgtacgatc
     3001 agactgtatg cttcacttgg cggccgatag atcattcctt catatctcat atcttatcct
     3061 gctttcttaa ttattttaca attcttctta cagaaagtac ttttcgataa ttagcctttg
     3121 gtgaaatctt aattcctgtt tttcttgaac ttgcatgaac aatctttcca ttgcctatat
     3181 aaatagcaat atgcccacta taacagatca gatctcctgg ctgtttatta ctgtaagaaa
     3241 ccttacgtcc tgcacttctt tgtgaggatg aacttcttgg aagattatat ccaaatgact
     3301 tatagacgct ttttacgaat cctgaacaat ccgctcccgt attcaagttt gttcctcccc
     3361 atacatatgg attcccaaca aatcgttgtg caaaacttgc aacagtttgt cctttgttat
     3421 aagtacttcc cgtaagtttt ggtgcatttg tactgatata aacatacttt tttaatgcat
     3481 atccagtttt gttaccaact ttaatttttc tccagcttcc acttgttgat aatactgtaa
     3541 cttttgttcc tacacttagt ttcttgatcg ttttagaacg tttagacttt gctgttctta
     3601 atttgcacca gtgtttataa atctttccac tgtaccctga tgctgcctga acggttgtcg
     3661 acgatgcttg atcagctatc gtaactgtcg atatcattat tacgactgtt attattataa
     3721 cagctacttt ctttattgtc tttatcataa ttctgcctca attctactct ttgatatttt
     3781 gttacatttc tgttacattt attaaattta acacattatt cttcttttgt aaagtgattt
     3841 tgtggtattc cgtaatattt ttgtaacatt ttacaaagta atcttgctaa aattcaaagg
     3901 gtaaaatctg acatacgctt tcttttc
//
LOCUS       DAOMUE010000189         3915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6525_length_3915_cov_2.258808, whole genome shotgun sequence.
ACCESSION   DAOMUE010000189 DAOMUE010000000
VERSION     DAOMUE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6525_length_3915_cov_2.258808"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PLC71_08200"
     CDS             <1..790
                     /locus_tag="PLC71_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRM02659.1"
                     /translation="ITEQNVNYALSLSMEEKSQSLLELDKDVICHTIQGKAVKPKTLG
                     QQKYVDAIKNKMITFGVGPAGTGKTYLAMAMAITAFKNDEVSRIILTRPAIEAGEKLG
                     FLPGDLQSKVDPYLRPLYDALYEILGADAFMKNMEKGLIEVAPLAYMRGRTLDNAYII
                     LDEAQNTTPAQMKMFLTRIGFGSKAIITGDLTQKDLPFDSISGLEESLRVLRKVKEIG
                     VCELTNKDVVRHPLVQKIVAAYDQYEAARSAKKNKNNNNKKNRR"
     gene            798..1310
                     /gene="ybeY"
                     /locus_tag="PLC71_08205"
     CDS             798..1310
                     /gene="ybeY"
                     /locus_tag="PLC71_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRM02660.1"
                     /translation="MSIIIENEYEGEINIPYEKIAHEVIEAAIDYVDCPYECEVNLML
                     TDNETIHAINKEQREIDRPTDVLSFPMAEYEEPADFSKIEEDPMAFDPESGEFLLGDI
                     VISMDKVWEQAENYGHSREREYAFLIAHSMLHLFGYDHMEEEERKDMEKRQEEILTKT
                     KYTRDERGLV"
     gene            1307..>1709
                     /locus_tag="PLC71_08210"
     CDS             1307..>1709
                     /locus_tag="PLC71_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRM02661.1"
                     /translation="MSKSSKNSFLMQGMILAAASIVCRVIGLLYRSPLFGIIGKEGMG
                     YYSYAYNIYNIILLIASFSIPLAVSKSISARLAKKEYINAQRIFYGALVYAVVVGGIA
                     SMIAYFGAPTFVGHQKGAALALQVISPTIFFS"
     assembly_gap    1710..1719
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1720..2944
                     /locus_tag="PLC71_08215"
     CDS             <1720..2944
                     /locus_tag="PLC71_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HRM02662.1"
                     /translation="GYFQGHNTMVPTSISQIIEQILNAIFSILMAYVLILPYLPSNKI
                     TYEIARHGAAGSAIGTGVGVLTGLIFCSIIYRAYQPKVKIQMKHDRTKYIESYGNILK
                     ILLLTITPVIFSTAIYNCSATIDQTIFSNILVGKGITAKVVSEFYGLFSGQYNVLINV
                     PVAMASALSNAIVPNVSAAYALGKKDDMNDSIQMAIRFTMMIAIPCAVGLGVLNSSIN
                     GLIFGATYAKGLGAAMLRIGAVSVIFYCLSTLTNGILQGMGKMRVPVRHSAISVVVNI
                     VVLWLLLTYTDCKTYGLVFATMAFSLVMCLLNAHSIKKYTGFHQEITKTFIKPALSAA
                     VMGVVCFLIQYAVQSVLPFGRVCFAVCVIIAVPVGALIYFAAIIKLKVFTEEELTKIP
                     KGNLIVRLAKKLHLL"
     gene            3062..3427
                     /locus_tag="PLC71_08220"
     CDS             3062..3427
                     /locus_tag="PLC71_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02663.1"
                     /translation="MKKINRFLMIYMVVITVLCICMCYLAFSANEKKDNLKEVRSQIT
                     TTAEKKQTKAVKKVKATYSLKILDGSLAVINNKTKEVFEYTDMKKENLPADIKNMLKE
                     NFIFENREEVYHFLESYSS"
     gene            3454..>3915
                     /locus_tag="PLC71_08225"
     CDS             3454..>3915
                     /locus_tag="PLC71_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRM02664.1"
                     /translation="MKYDVIIVGAGASGMMAAITAASHHKKVLLLERMDKLGKKILAT
                     GNGRCNLSNAYMDENCYRGQRPSFAYPMIEEFGLEDLIQFFESLGMLVTEQNGYYYPA
                     SLQAATVVDTLTQKLKHLHVDILTEFEVTKAEKRKSTFYVYGNDKCFQSSNL"
BASE COUNT         1268 a          661 c          887 g         1089 t
ORIGIN      
        1 gatcacagag cagaatgtaa actatgcgtt atcgttatca atggaagaga aatctcaaag
       61 tcttttggag cttgataaag atgtcatctg tcatacgatc cagggcaaag cagtcaaacc
      121 aaagacttta ggacagcaaa aatacgtcga tgcgatcaag aataagatga tcacatttgg
      181 agtcggtcct gcaggaactg gtaagacata tcttgcaatg gcaatggcga tcacagcatt
      241 taagaatgat gaagtcagcc gtatcatttt aacaagacca gcaattgagg caggagagaa
      301 gctaggtttc ttgcctggag atctacagag taaagttgat ccatatttaa gaccattata
      361 tgatgcatta tatgagatcc ttggggcaga cgcatttatg aagaatatgg agaaaggctt
      421 gatcgaagtc gctccattgg cttacatgag aggacgtaca ctggataatg catacattat
      481 cttagatgag gcacagaata cgacacctgc tcagatgaag atgttcttaa cacgtattgg
      541 atttgggtcc aaagcgatca tcacgggtga cttaacacag aaagacttgc catttgacag
      601 tatttcagga ttagaagaat ctctaagagt gttaagaaaa gttaaggaga tcggggtctg
      661 tgagcttacc aataaggacg ttgtcagaca tccattagta cagaagatcg ttgcagcata
      721 tgatcaatat gaagcagcca gatctgcgaa aaagaataag aataacaata acaaaaagaa
      781 cagaagatag gaatgttatg agtattataa tcgagaatga atacgaaggg gagatcaata
      841 tcccttatga aaagatcgca catgaagtga tcgaggcagc aattgattat gtggactgcc
      901 cttatgagtg cgaagtgaat ttaatgctaa ccgataatga aacgattcat gcgatcaaca
      961 aagaacaaag agagatcgac cggccaacag acgtactgtc ttttccaatg gcagaatacg
     1021 aagaaccagc ggatttctca aagattgaag aagatccgat ggcatttgat ccggagagtg
     1081 gtgaatttct gcttggagat atcgtgatct ccatggacaa ggtatgggaa caggccgaga
     1141 attatggaca cagcagggag agagaatatg cctttttgat cgctcacagt atgttacatc
     1201 tgtttggata tgaccatatg gaagaggaag aacgaaaaga tatggaaaaa cgtcaggaag
     1261 agattttgac aaagacgaaa tatacaagag atgaaagagg tttagtatga gtaaaagcag
     1321 caaaaactca tttttaatgc aaggaatgat cttagccgca gcatccattg tctgccgtgt
     1381 gatcggatta ttatatcgta gtccactgtt tgggatcatc ggcaaagaag gtatggggta
     1441 ctattcttat gcgtataata tctataacat tattctgctg atcgcatcct ttagtattcc
     1501 acttgcggta tccaaatcaa tttctgcaag acttgcgaag aaagaatata tcaatgctca
     1561 gagaatcttc tatggagcat tagtttatgc agttgtcgtt ggtggtatcg cgtccatgat
     1621 cgcatatttt ggagcaccaa cctttgtcgg acatcaaaaa ggtgcagcac ttgcccttca
     1681 ggtcatttcc ccaacgatct ttttctctgn nnnnnnnnna ggatattttc aaggacataa
     1741 tacaatggtg ccaacatcca tttcccagat cattgagcag atcttgaatg cgatctttag
     1801 tattttgatg gcatatgtac tgatcttacc atatctgcca tctaacaaga tcacatatga
     1861 gatcgcaaga catggggcag caggtagtgc gatcggtaca ggagtcggtg ttttaacagg
     1921 attgatcttc tgctctatca tttatcgtgc ataccagcca aaggtcaaga ttcagatgaa
     1981 acatgaccgt acaaaatata ttgaatctta tgggaatatc ttaaagatct tattattaac
     2041 gatcacaccg gttatcttta gtacagcgat ctataactgt agtgcaacga tcgaccagac
     2101 gatctttagt aatatcttag taggaaaagg aattacggcg aaagtagttt cagaatttta
     2161 tggtttgttc agcggtcagt ataacgtgct gatcaatgtt ccagttgcga tggcatctgc
     2221 actttctaat gcgatcgtgc caaatgtttc tgcagcttat gcgttaggca agaaggacga
     2281 tatgaacgac agtatccaga tggcgatacg ttttacgatg atgatcgcga tcccatgtgc
     2341 ggttggacta ggtgtgttga atagttcgat caatggattg atcttcggtg caacatatgc
     2401 taagggatta ggagctgcca tgcttcggat cggtgcagtt tccgttattt tctactgtct
     2461 gtcaacgttg acaaatggta tcttacaggg aatgggaaag atgagagtgc cagtcagaca
     2521 ttctgcgatt tccgttgtgg tcaatatcgt tgtattatgg cttttattaa catacacaga
     2581 ttgtaaaaca tatggattgg tatttgcgac tatggcattt tcccttgtga tgtgtctatt
     2641 aaatgcacac tccatcaaga aatacacagg ttttcatcag gagatcacaa agacatttat
     2701 caagcctgca ttaagtgcag cagtgatggg tgttgtctgt tttctgattc agtatgcggt
     2761 tcaaagcgtt cttccattcg gacgagtatg ctttgcggtc tgcgtgatca ttgcagtgcc
     2821 agttggagca ctgatctatt ttgcagcgat cattaagtta aaagtattta cagaagaaga
     2881 acttactaag attccaaaag gaaatctgat cgtcagatta gcaaagaaat tacatttgct
     2941 gtaaggatcg taagggcttc catttataaa aggaagaaaa agtccagatc tgctatgatt
     3001 tgggcttttt gtataaattc ccttatcttt ggtaacaata tagccaaagg agggttgcat
     3061 tatgaagaag atcaatcgat ttttgatgat atatatggtt gtgatcacag ttttgtgcat
     3121 ctgtatgtgt tatcttgcat tctcagcaaa tgaaaagaag gacaacctca aggaagttcg
     3181 ttctcagata acaacaacag ctgaaaaaaa acagaccaaa gcagtcaaga aggttaaggc
     3241 aacgtacagt ctgaagattc ttgatggaag tctggctgtg atcaataata agaccaaaga
     3301 agtttttgaa tatacagata tgaagaaaga gaatctgcca gcagacatca aaaatatgct
     3361 gaaagagaat tttatctttg aaaacagaga agaagtatat cattttctgg aatcatacag
     3421 tagttaaaat aaaaatttgg agaaaaataa cgaatgaaat atgatgtgat catcgttgga
     3481 gcaggggctt ctggtatgat ggcagcgatc acagcagcta gtcatcataa gaaagtcctc
     3541 ttgcttgaac ggatggataa attaggaaag aaaatattag caacgggaaa tggaagatgc
     3601 aacctgtcaa atgcctacat ggacgagaat tgctacagag ggcagagacc ttcctttgcc
     3661 tatccgatga tcgaggaatt tggattagaa gatctcattc agttctttga atctctcgga
     3721 atgcttgtga cagagcaaaa tggatattat tatcctgcat ctttgcaggc agcaactgtt
     3781 gttgatacat tgacacagaa attaaagcat ctgcacgtgg atattctgac agaatttgaa
     3841 gtcacgaaag cagagaaacg taagtccaca ttttatgtct atggaaatga caaatgtttt
     3901 cagtcaagca atctg
//
LOCUS       DAOMUE010000190         3902 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6554_length_3902_cov_2.557837, whole genome shotgun sequence.
ACCESSION   DAOMUE010000190 DAOMUE010000000
VERSION     DAOMUE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6554_length_3902_cov_2.557837"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..761
                     /locus_tag="PLC71_08230"
     CDS             6..761
                     /locus_tag="PLC71_08230"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HRM02665.1"
                     /translation="MYQIDLNSDLGESFGRYTIGNDDKIIPLITSANVACGYHASDPV
                     VMMETIQRAKEAGIAVGAHPGLPDLMGFGRRSMAISNEEAKAYTLYQISAIGGMCKAA
                     GVKLQHVKPHGALYNMAAKDYDLSKAICEAIYEYDKELIVMGLSGSEMIKAAKDCGLK
                     SASEVFADRAYEEDGTLVNRRKPGAMIEDEDEAINRVIRMIKEGKVTSITGKDIDIKA
                     DSVCVHGDGEKALLFVEKIRKTFGENEIEICHL"
     gene            958..2421
                     /locus_tag="PLC71_08235"
     CDS             958..2421
                     /locus_tag="PLC71_08235"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HRM02666.1"
                     /translation="MQDKVRDLRSALKRLKQMEGQYIETDVEVDPMAELAGVYRYVGA
                     GGTVKRPTKEGPAMVFNNVKGHKDARVAIGVLASRKRVAALLDCKPEELGKKLYHSVD
                     NPIAPVEYQGDAPCQQVVHKVEDPDFNLYDLVPAPTNTPDDAGPYITLGMCYATHPDT
                     GVHDVTIHRLCIQGKDELSIFFTPGARHIGAMAERAEELGQKLPISISIGVDPAIEIG
                     SCFEAPTTPLGYDELSVAGALRNEPVELCKCLTVNEMAIANAEYVIEGEVIPNVRVQE
                     DQNSHTGYAMPEFPGYTGPASSQCWLIKVKAVTHRENPIMQTCIGPSEEHVSMAGIPT
                     EASIYGMVEKAMPGRLQNVYCGSAGGGKYMAVLQFKKREASDEGRQRQAALLAFSAFS
                     ELKHVFLVDEDVDCFDMNDVLWAMNTRFQGDADIITIPGVRCHPLDPSNDPTCSSSIR
                     DHGIACKTIFDCTVPYDQKERFKRARFMEVDPEHWLS"
     gene            2459..3028
                     /locus_tag="PLC71_08240"
     CDS             2459..3028
                     /locus_tag="PLC71_08240"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HRM02667.1"
                     /translation="MKKRLIIGATGASGIPILIQCLKLVQVQKDFESYLVMTDSAKLT
                     LEHETKMKVEEVEKLADHVLDSREIGAGPASGSFQTEGMLIVPCSMKTIAGIHSGYAD
                     NLILRAADVTIKEQRPLVLAARETPLSAIHLRNLQELAMIPNVRIIPPMMTFYHMPES
                     IEEMTYHIAAKLLEPFGIEAKEYRRWSGL"
     gene            3043..>3902
                     /locus_tag="PLC71_08245"
     CDS             3043..>3902
                     /locus_tag="PLC71_08245"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HRM02668.1"
                     /translation="MKILSFGSLNIDYVYKVSHFVKKGETLSAEELNVYTGGKGLNQS
                     IALSRAGMETYHAGAIGMDGLFLLDQLKEAGVNTDLVKILEDVRTGNAIIQNDEEGDN
                     CIILFGGANQAISKEQVDEVFEHFKNEDYLLIQNEINELPYIVKKAKEIGMKIILNPS
                     PMNEKIKELPLDQINYFLLNEIEAMQILDMEEPKEIDGKYISGLLHQKFPEATIVLTL
                     GSEGSVCISGDEYVEQSIYKVKTVDTTAAGDTYTGYFIAGILNGKTIKEAMNTASKAS
                     AIAVSRQGAAP"
BASE COUNT         1382 a          628 c          935 g          957 t
ORIGIN      
        1 attaaatgta ccagatagat ttaaatagtg accttggaga aagttttgga cgttacacga
       61 tcgggaatga tgataagatc attccattga tcacatcagc gaatgttgca tgtggttatc
      121 atgcatccga tccagtggtt atgatggaga cgatccagag agcaaaagaa gccgggatcg
      181 cagttggggc ccatccggga cttccagatc tgatgggatt tggacgaaga agcatggcga
      241 tttccaacga agaagcgaaa gcatatacac tataccagat cagtgcgata ggtggaatgt
      301 gtaaagcggc cggagtgaaa ttacagcatg taaaaccaca tggagcatta tataatatgg
      361 cggctaagga ttatgatctg tctaaagcga tctgcgaagc aatttatgaa tatgataaag
      421 aactgatcgt tatgggatta tctggaagcg agatgatcaa agcagcaaaa gactgtggat
      481 taaagtcagc cagtgaagta tttgcagaca gagcttatga agaagatgga acactggtga
      541 atcgaagaaa accaggagct atgatcgaag atgaagatga agcgatcaac cgagttattc
      601 gtatgataaa agaaggaaaa gtgacttcga tcactggaaa agatattgat attaaggcag
      661 actcagtatg cgttcatgga gatggagaga aagcattatt atttgtggag aagatccgta
      721 agacattcgg tgagaatgag atagaaatct gtcatttata atggagatat ttttaagata
      781 atgaagaaat ggtagatgga tctagagaag aattcatctg ccattttttc atttcatagc
      841 aatttgtgac tctattgatg ccatgtcatt gaactgttgt tatcgaaaag cagtacttga
      901 acattgggat tatgttgttg gaaatgagaa agagaagtat aagaaagaag gagacaaatg
      961 caagataaag taagagattt aagaagtgca ttaaaacgct tgaaacaaat ggaagggcag
     1021 tacatagaaa ctgatgtaga agttgatcca atggcagaac ttgcgggagt ttaccgctat
     1081 gtaggtgcag gtggaactgt aaagcgcccg acaaaagaag gtccagcgat ggtatttaat
     1141 aatgtcaaag gacataaaga tgccagagta gccatcggtg tattagcaag cagaaagcgt
     1201 gtagcagcgc tcttggactg taaaccagaa gaattaggaa agaaacttta tcatagcgta
     1261 gataatccga tagcaccagt ggaatatcag ggagatgcac catgtcagca ggttgttcat
     1321 aaagtggaag atccagactt taatttgtat gatctggtac cagcaccaac caacacacca
     1381 gatgatgcag gcccatatat tacattgggt atgtgctatg caacgcaccc agacacagga
     1441 gttcatgatg taaccatcca cagattatgc attcagggaa aagatgaact ttctattttc
     1501 ttcactcctg gagcaagaca catcggagca atggcagaac gtgcagaaga attaggacag
     1561 aaacttccaa tttcaatcag tatcggagtg gatccggcga tcgagatcgg ttcttgtttc
     1621 gaggcaccaa caacacctct gggatacgat gaattatctg tagcaggtgc attgagaaat
     1681 gaaccagtag agctttgtaa atgcttaaca gttaacgaaa tggcaattgc aaatgccgaa
     1741 tatgtcatcg aaggggaagt gattccaaat gtccgagtac aggaagacca gaacagccac
     1801 acaggctatg caatgccaga atttccagga tacacaggac ctgcaagttc acagtgctgg
     1861 ctgatcaaag tcaaagcggt aacacacaga gaaaatccga tcatgcagac atgcatcggg
     1921 ccaagtgaag aacatgtatc tatggctgga atcccaacag aagcaagtat ttatggaatg
     1981 gtagaaaaag cgatgccggg acgtcttcag aatgtatact gcggctccgc aggcggtgga
     2041 aaatacatgg cagtcttaca gtttaagaaa cgagaagcca gcgatgaagg aagacaaaga
     2101 caggcagcac ttcttgcatt ttcagcattc agtgaactga agcatgtatt tttggtagac
     2161 gaagacgtcg actgtttcga tatgaatgat gtattatggg caatgaatac aagattccag
     2221 ggagatgcag atatcatcac gattccaggg gtaagatgtc atccgctgga tccatccaac
     2281 gatccaacat gttcttcaag tatcagagat catgggattg catgcaagac aatctttgac
     2341 tgtactgttc catatgatca gaaagagcga ttcaagagag caagatttat ggaagttgat
     2401 ccggaacact ggttatcata aaatacagaa tataaatagg aaaaggaaat ttatttgaat
     2461 gaagaaacga ctgatcatag gagcaacagg ggcaagtgga attccaatct tgatccagtg
     2521 tctaaaattg gtgcaagtgc agaaggattt tgaatcatat ttggttatga cagatagtgc
     2581 caaactgacc ttggaacatg aaacaaaaat gaaggtggaa gaggttgaaa aacttgctga
     2641 tcatgttctg gattcaagag aaatcggtgc aggaccagcc agtggatcat ttcagacgga
     2701 agggatgctc attgtcccat gtagtatgaa gacaattgca ggaattcaca gtggatatgc
     2761 agacaatctg atacttcgtg cagcggatgt aacgatcaaa gagcaacggc cattggtgct
     2821 tgcagcaaga gagacaccac tgagtgcgat tcatcttaga aatcttcagg aattggcaat
     2881 gattccgaat gtgcggatca ttccaccaat gatgactttt tatcatatgc cagagagcat
     2941 agaagaaatg acgtatcata ttgcagcaaa actattagaa ccatttggga tagaagcaaa
     3001 agaatatcga agatggagcg gattgtagaa agcgaggttt ccatgaaaat attatcattt
     3061 ggatcattaa atattgacta tgtatataaa gtttctcatt ttgtgaaaaa aggagaaaca
     3121 ttgtctgcag aagagttgaa tgtatataca ggcggaaaag gattaaacca gtccattgca
     3181 ctttcacgtg caggaatgga aacatatcat gcaggagcga ttggcatgga tggactattt
     3241 ttgttagatc aattaaaaga agcaggagta aatacagatc ttgttaagat ccttgaagac
     3301 gtaagaacag gaaatgcgat catacaaaat gatgaagagg gcgataactg tattattcta
     3361 tttggaggag ctaatcaggc aatcagtaag gaacaagtag atgaagtctt tgaacatttt
     3421 aaaaacgaag attatttact gatccaaaat gagatcaatg aacttccata tatcgtaaag
     3481 aaagcaaaag aaattggaat gaagatcatt ttaaatccat ctccaatgaa tgagaagata
     3541 aaagaacttc cactagatca gatcaactat tttcttttaa atgaaataga agcaatgcag
     3601 attcttgata tggaagaacc aaaagaaatt gatgggaaat atattagtgg tctgttacat
     3661 cagaaattcc cagaagcaac catcgtatta actttaggaa gtgaaggatc tgtatgtata
     3721 tctggtgatg aatatgtgga acagtcaatt tataaggtaa agacagtcga tacaacggca
     3781 gcaggagata cctataccgg atattttatt gcaggtatct taaatggaaa aacgattaaa
     3841 gaagcaatga ataccgcatc caaagcatca gcaattgcag taagcagaca aggggcagca
     3901 cc
//
LOCUS       DAOMUE010000191         3883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6602_length_3883_cov_2.336468, whole genome shotgun sequence.
ACCESSION   DAOMUE010000191 DAOMUE010000000
VERSION     DAOMUE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6602_length_3883_cov_2.336468"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLC71_08250"
     CDS             complement(<1..731)
                     /locus_tag="PLC71_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRM02669.1"
                     /translation="MKTKAVRLYGEMDLRLDEFELPEMQDDEIMAEVVTDSLCMSSYK
                     AITQAQKHKKVPDDISENPIILGHEFCGTIKKVGKKWKDQYQEGDKFVIQPNIGDIRG
                     YAPGYSFHHMGGDATTIIFSDQVMENGSLLKYNGDSFFEGSLVEPLSCVVGAFNAQYH
                     MKKMYSYEHVMGIKEGGAIALLGATGPMGFLAIDFAIHGPKKPKTLVVTGRTQKKIDM
                     AARLYTVEEAKKNGVKLVYVNTRDIV"
     gene            complement(750..1571)
                     /locus_tag="PLC71_08255"
     CDS             complement(750..1571)
                     /locus_tag="PLC71_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HRM02670.1"
                     /translation="MSEKKLTKGDRFNMFMRTNLQQASFNYERMHALGMCFDMVPAIK
                     RLYDTKEEQVAALKRHLTFFNVTPAAVGPVLGVTAAMEEAKANGADIDEGTIGSIKIG
                     LMGPFCGVGDPIFWGTLRPITAAIGASLALTGSALGPVLFFLAFNIVRMAALYFGLEF
                     GYKKGLDFVKHLAGNMLQKVTEGASILGLFVMGALVSKWTTINVPLVVSRVTTNGKTT
                     VTTVQNILDQLLPGLLALVLTLVVSKLLKKNISPIAIIFILFAVGIVGYGLGILA"
     gene            complement(1592..2410)
                     /locus_tag="PLC71_08260"
     CDS             complement(1592..2410)
                     /locus_tag="PLC71_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HRM02671.1"
                     /translation="MGISTVQIVLLVIFGCIAGMGSVLDSFQTHRPLIACTVVGLILG
                     DVKTGILLGGTLEMIALGWMNIGAAQSPDSALASIISTILVVVGHQSVANGIAIALPV
                     AVAGQVLTVFARTVTIFFQHAADKYAEDANFKMLDICHVAALVVQALRVAIPTFLVAA
                     VVSPEGVQTLLNSIPKVVTGGLTVAGGMIVVVGYAMVLNMMGAKYLMPFFFLGFVIAA
                     FTEFNLVAFGIMGAVFAIVYVQLNPKFQTPVAAPVTAGAGGSDDLDDDLDDELD"
     gene            complement(2436..2924)
                     /locus_tag="PLC71_08265"
     CDS             complement(2436..2924)
                     /locus_tag="PLC71_08265"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HRM02672.1"
                     /translation="MKINLVRIDDRLIHGQVATVWSKEANAQRIIICSDEVAKDQIRK
                     TLLLQVGPPGVKVSVVDVAKAVRVYNNPKYEKDTVFFLYTCPQDVLRMAEAGVPIKSV
                     NIGGMAFKTGKTQITKAVSVDESDIAAFKKLHEMGIELEIRPVATDKKVDLMSKIEGI
                     EG"
     gene            complement(2921..3352)
                     /locus_tag="PLC71_08270"
     CDS             complement(2921..3352)
                     /locus_tag="PLC71_08270"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HRM02673.1"
                     /translation="MKLIVSAHGMLAKEVVNSAGMVFGAIDDLDIVTFVPGENAETLK
                     ARYKELIDGYKEDEEILFLVDLFGGSPYNAAFETVIGQDRMDVITGLSLPMLIDVVGI
                     RTMEEDIKAGEVYDKLSGDYVKSCKKLLGNTNEDEMEDDEL"
     gene            complement(3611..>3883)
                     /locus_tag="PLC71_08275"
     CDS             complement(3611..>3883)
                     /locus_tag="PLC71_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02674.1"
                     /translation="IKEHPDDGEIYCTKYAVLAKQEKYTQGLKVIERALKQKELENKK
                     EVLFARISAYESMFDFNTAYRYAKAYVKAYPKDANGKKELTFLETR"
BASE COUNT         1010 a          894 c          614 g         1365 t
ORIGIN      
        1 actatatctc tcgtattgac atatacaagc tttacaccat tcttctttgc ttcttctact
       61 gtgtataatc ttgctgccat atcaatcttc ttctgtgttc ttcctgtgac aaccagcgtc
      121 tttggtttct ttggtccatg gattgcaaag tcgatcgcta aaaatcccat tggccctgtt
      181 gcaccgagaa gtgcgattgc tccaccttct ttaattccca tcacatgttc atatgaatac
      241 attttcttca tatgatactg tgcattaaat gcacctacga cacatgacag tggttcaacg
      301 agagatcctt caaagaagga atctccgttg tattttaata gtgaaccatt ttccataacc
      361 tgatcagaaa agatgatcgt tgtggcatcc cctcccatat gatggaaaga atatcctggg
      421 gcatagcctc tgatatctcc aatgttaggc tgaatgacaa atttgtcacc ttcctgatac
      481 tgatctttcc attttttacc gacctttttg atcgttccac aaaattcgtg tcctaaaatg
      541 atcggatttt cactgatatc atctggtact tttttgtgtt tttgtgcctg ggtgatcgct
      601 ttgtaactgg acatacacaa actgtctgtt acaacctctg ccataatttc atcatcttgc
      661 atctctggca gttcaaattc atccagtctt aaatccattt ctccgtaaag tcttactgcc
      721 tttgttttca taactttccc cctaacagat tatgccaaaa ttcctaatcc atatccaaca
      781 attcctactg caaataagat aaagataatt gcgatcggac tgatgttttt ctttaataat
      841 ttagatacga ccaaggttaa aactaatgct aataatcctg gtaataactg atctaagata
      901 ttctgtactg ttgttactgt tgtttttccg tttgttgtta ctctagatac tacaagtggt
      961 acgttaatcg tcgtccattt agataccagt gcacccatga cgaacagtcc aaggatagaa
     1021 gctccttctg tgactttctg taacatattt cctgctaaat gttttacaaa gtctaatcct
     1081 tttttgtatc cgaattcaag tccaaagtaa agtgctgcca tacgtacgat gttgaatgct
     1141 aagaagaata atactggtcc taatgcactt cctgttaatg ctaaggatgc accgatcgct
     1201 gctgtgatcg gtcttaatgt tccccagaag attggatcac caactccaca gaaaggtccc
     1261 attaacccaa tcttgatact tccgatcgtt ccttcatcga tatctgctcc atttgctttt
     1321 gcttcttcca tcgctgctgt tacacctaag actggtccta cagctgctgg tgttacgttg
     1381 aagaatgtca gatgtctctt taatgctgct acttgttctt cttttgtatc atataatctt
     1441 ttaatagctg gtaccatgtc gaagcacata cctaatgcat gcattctttc gtagttaaat
     1501 gatgcttgct gaaggtttgt tctcatgaac atattaaatc tatcgccttt tgttaatttc
     1561 ttttcactca tctttaaatt cctcctcttt cttagtcaag ttcgtcgtct agatcatcat
     1621 ccagatcatc gctgccacct gcacctgctg ttactggtgc tgccactggt gtctggaatt
     1681 taggatttaa ctgtacatat acgattgcga ataccgcacc catgattccg aatgcaacta
     1741 agttaaattc tgtaaacgct gcaattacga atccaaggaa gaaaaatggc attaagtatt
     1801 ttgcacccat catgtttaat accattgcgt aacctacgac tacgatcatt ccacctgcaa
     1861 ctgttaatcc accagttact actttcggaa tgctgtttaa taatgtctgt acaccttctg
     1921 ggcttacaac tgctgctact aagaatgtag ggattgctac acgaagtgcc tgtacaacca
     1981 atgcggctac atgacagata tcaagcatct tgaagtttgc atcttctgca tatttgtcag
     2041 ctgcgtgctg gaagaagatt gttactgtac gagcaaatac tgttaacacc tgacctgcaa
     2101 ctgcaactgg aagtgcgatc gcgataccat ttgctacaga ctgatgtcct acaactacaa
     2161 gaatcgtaga aatgatactt gctaatgctg agtcaggaga ctgtgctgct ccgatgttca
     2221 tccatccaag agcgatcatt tccagtgtac ctccaagtaa gatacctgtt tttacatctc
     2281 ctaagatcaa tccaacaact gtacatgcga tcagtggacg atgtgtctgg aaggaatcaa
     2341 gcacgcttcc cattccagcg atacatccaa aaataactaa taaaacgatt tgaactgtag
     2401 atattcccat ttttaaatcc tcctttgtat ctttcttatc cttcgatgcc ttcgatcttg
     2461 ctcataagat ctactttttt gtctgtggct accggacgga tctctaattc aattcccatc
     2521 tcatgcagtt tcttaaatgc tgcgatatcg ctttcatcaa cagatactgc ctttgtgatc
     2581 tgagtctttc ctgtcttgaa tgccattcct ccgatgttaa cactcttgat cggaacccct
     2641 gcttctgcca ttcgcagaac atcctgtgga catgtatata agaagaatac tgtgtccttt
     2701 tcatattttg gattgttgta aacacgaact gcttttgcta cgtctacaac gcttactttt
     2761 actcctggag gtcccacctg taaaagtaat gttttacgga tctggtcttt tgctacttca
     2821 tcactgcaaa tgatgattcg ctgtgcgttt gcttctttgg accaaacggt tgcgacctgt
     2881 ccatggatca gtctgtcatc gattcttact aaattgattt tcatagttca tcatcctcca
     2941 tttcatcttc gtttgtatta ccaagcaatt tcttgcatga tttcacataa tctcctgaaa
     3001 gtttgtcata tacttcgcca gccttgatat cttcttccat tgttctgatt cctacaacat
     3061 cgatcagcat tggtaagctc aatcctgtaa taacatccat tcgatcttgt ccgatcacgg
     3121 tctcaaatgc tgcattataa ggacttcctc caaataaatc tacaaggaat aaaatttctt
     3181 cgtcttcctt atagccatcg atcaattctt tgtatctggc ttttaatgtc tctgcattct
     3241 cacctggaac aaatgttacg atgtcaagat catcaattgc tccgaacacc attcccgcag
     3301 aattcacaac ctcttttgcc aacattccat gggctgatac aattaacttc attgctctcc
     3361 ctcctttagc acgaaattta tttttttact tttgtctcat gtgaaatttt attttgtatc
     3421 ttgattatag tcataaacct aaatttcgtc aacttatttt tcgtaaaatt ttattttttg
     3481 tataaagtca tgatttttgc tttctatttt tgtatatttt gcacaatttt caattgatct
     3541 tttctgtttc tgcacaaaaa aaagaaggac tttcgtcctt cctaactctt atcatgaaat
     3601 tgtcacgaaa ttatcgtgtc tccaaaaatg tcagctcttt cttgccattc gcatcctttg
     3661 gataggcttt tacataagct tttgcataac gatatgctgt attgaaatca aacatggatt
     3721 catacgcact gatcctagcg aataaaactt ccttcttatt ctcaagctcc ttctgtttta
     3781 atgctctctc gatcactttt agcccttgtg tatacttctc ctgctttgct aacaccgcat
     3841 attttgtaca atagatttct ccatcgtctg gatgctcttt tat
//
LOCUS       DAOMUE010000192         3880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6607_length_3880_cov_2.276863, whole genome shotgun sequence.
ACCESSION   DAOMUE010000192 DAOMUE010000000
VERSION     DAOMUE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6607_length_3880_cov_2.276863"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..2008
                     /locus_tag="PLC71_08280"
     CDS             53..2008
                     /locus_tag="PLC71_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM02675.1"
                     /translation="MNNLQEKVIRAMVDMYGFYSIEELSEMTEISISSIKRSLKDITK
                     FIEDYDGKLLRVPKKGICMVVTDQQRENILEALDSYANGNPESFYYRKNYILDILFNY
                     PANYTVQLFSEELCVSRKMIEKDLKMIEEYLATFHLELSKVQNQGISIVGREFDIRQA
                     IVDTQNKKYWKHTYIEKLPQELDYRISKRAFTYFSDYYSKEDIMMVQEYLADSEAELG
                     VVLVDISFCRLTEYLLLTKRRMEEGNKIRDKADRTMTKLDDIYVETAKKILDRMFPHE
                     DEMELEYQFLAAKFAVAKTCEVEAEIQDDYLMNIAEDYIYTVFMAMEKENTYHYRELE
                     KQIATFLKKISIRHDYMLVEWDDLHKDVQQQIQSSYAVCLTYSFHLEEKLGFVLTQDE
                     IAWLALLIHQASMETGSEKQGIFVMATDPYSAKYEAMKIENEISNLEIIKIVHIHDFK
                     PELTKGKLVISTVPLKEKRNNVIEITKHVTQLDINKIISQMDEYAQEMKNEQVIETVK
                     KAFQKELIVIDALATTKKETIAQVSKLLLEQGCLSEDVTEKVFELEERRPTTIGSQIA
                     MAHVYKDSVKKSGIAVMRMKYPVKWSRSSKVKLVFFLAINMEESNEILKLFKYLYALI
                     DNKEDIQKILEANSSEEIYELLMEEFH"
     gene            2051..2932
                     /locus_tag="PLC71_08285"
     CDS             2051..2932
                     /locus_tag="PLC71_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRM02676.1"
                     /translation="MLVNMKKILEIAEAEGYAIPCINTPNEETVRAVIGAAEELNTPI
                     IIDHAQVHDSLIPIERIGPIMLKYAKEARVPVCVHLDHGSDYEFVMRAVKVGFPSIMY
                     DCSALPFEENVEKVVQFVKFAHAAGITVEAELGIMASTDEDTHDDDPEKRVLTNEDIR
                     EFFTEPAEAKEFAERTKCDALAVCFGTMHGIYAEPPVLDIDRVKELREAIPDDTRIVM
                     HGASGVEFDQVQNAITAGCSKVNYYSYMAKATTKFVADKVAETDGKIAYHELQEAAYE
                     FMKGYAKDVIKAFKNGK"
     gene            2960..3433
                     /locus_tag="PLC71_08290"
     CDS             2960..3433
                     /locus_tag="PLC71_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM02677.1"
                     /translation="MSGVNLAEVLLDEMVILDCEPFKDKENMFDVMTAKFEEAGFVSD
                     AKAYKDALEYRESLGPTYMGNFVAIPHGKCKEVLKPGIGFCRCKKPFIYESSGESGEV
                     KYIFVLAISENQENDYHLRVLATLAGMLAHKEFLELLEKAESYDDLIHGIKQLQL"
     gene            3455..3754
                     /locus_tag="PLC71_08295"
     CDS             3455..3754
                     /locus_tag="PLC71_08295"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIB"
                     /protein_id="HRM02678.1"
                     /translation="MKVVAVTSCATGIAHTYMAAEAIMKVCKERGYKCKVEKQGALGL
                     EDKLRDRDIKEADLIVFANDVGLTKAERFEPYKDKIFQTKPHEVIKDPNIIFSGK"
BASE COUNT         1490 a          587 c          819 g          984 t
ORIGIN      
        1 acagcagatg aaaagctact ttttagtgtt ttattcattg aggaggccta tcatgaataa
       61 tttacaggaa aaagtaatcc gtgcaatggt tgatatgtat ggattctatt ccatcgaaga
      121 gttatcggag atgactgaaa tttccataag cagtatcaaa cgatccttaa aagatattac
      181 aaaatttatt gaagactatg atggaaaact actaagagta cccaaaaaag gaatctgcat
      241 ggtggtgaca gatcagcaga gagaaaatat tttggaagca cttgattcct atgcaaatgg
      301 aaacccagaa tctttctact acagaaagaa ttacatttta gatatcttat tcaattatcc
      361 ggcaaattac acagttcaat tattttccga ggaattatgt gttagccgaa agatgataga
      421 aaaagatctg aaaatgatag aagaatatct ggcaaccttt catctggaat tatcaaaagt
      481 tcaaaatcag ggaatcagta ttgtaggtag agagtttgat atcaggcagg caattgttga
      541 cacacagaat aagaaatact ggaaacacac gtacattgaa aaacttccac aggaactaga
      601 ttacaggatc agtaaaagag catttacata tttttcagat tactattcca aagaagatat
      661 catgatggtt caggaatatc ttgcggatag tgaagcagaa ttaggagttg tgcttgtaga
      721 catttctttc tgcagattga cggaatacct tctattaact aagagaagaa tggaagaagg
      781 aaacaagatc agagataaag cagacagaac aatgacaaag ctcgatgata tatatgtaga
      841 aacagcaaag aagattcttg accgcatgtt cccacatgaa gatgagatgg aacttgaata
      901 ccagttcttg gcagcaaagt ttgctgtagc aaaaacttgt gaagtagaag ccgagattca
      961 ggatgattac ctgatgaata ttgcagaaga ttacatctac acagttttta tggcgatgga
     1021 aaaagaaaat acctatcact atcgggaact ggaaaaacaa attgctactt ttttaaagaa
     1081 gataagtatc agacatgatt acatgttagt tgaatgggac gatcttcaca aggacgtaca
     1141 gcaacagatt cagagcagtt atgcggtttg tttaacttac tctttccatt tggaagaaaa
     1201 acttggattt gtattgacac aggatgagat tgcctggctc gcattactga tccatcaggc
     1261 atccatggaa acaggaagtg aaaaacaagg aatctttgtt atggcaacag atccttattc
     1321 agcaaaatat gaagcaatga agattgagaa tgaaatcagt aatttagaaa tcataaaaat
     1381 cgtacatatt cacgatttta aaccagaatt aacaaaaggg aaactggtaa tatcaaccgt
     1441 tccactcaaa gaaaaaagaa ataatgtcat agaaatcaca aaacatgtta cgcagctgga
     1501 catcaataag atcatcagtc agatggatga gtacgcacag gaaatgaaga atgaacaagt
     1561 tatagaaact gtgaagaaag catttcagaa agaattgatt gtgattgatg cactggcaac
     1621 aactaaaaag gaaactatcg cacaggttag taagttatta ctggaacaag gatgtcttag
     1681 tgaagatgtc acagaaaaag tatttgaatt agaagaaaga agaccgacaa caattggaag
     1741 tcagatcgca atggcacatg tttataaaga ttccgtaaag aaaagcggca tcgcagtcat
     1801 gcgaatgaaa tatccagtga aatggtcgag atcatcaaag gttaaattag tatttttctt
     1861 agcaattaat atggaagaat caaacgagat cttaaaatta ttcaaatatt tatatgcact
     1921 gatcgataac aaagaagaca ttcaaaaaat cctagaagca aatagttccg aagaaattta
     1981 tgaactacta atggaagaat tccattagac gtaataacca acaaaactta tgttacagga
     2041 ggaagaacaa atgctagtaa acatgaaaaa aatcttggag attgcagaag cagaaggata
     2101 cgctatccca tgtatcaaca caccaaatga agaaacagtt cgtgcagtca ttggtgcagc
     2161 agaagaatta aatactccga tcatcattga ccatgctcag gtacatgact ctttgattcc
     2221 aatcgagaga atcgggccga tcatgttaaa atatgcaaaa gaagcaagag tacctgtttg
     2281 tgttcattta gatcatggta gtgactatga attcgtaatg agagcagtga aagttggatt
     2341 cccatctatc atgtacgatt gcagtgcact tccttttgaa gagaatgttg aaaaagttgt
     2401 acagttcgtt aaattcgcac atgctgcagg aatcacagta gaagcagaac ttggaatcat
     2461 ggcatctaca gatgaagata cacatgatga tgatccagaa aagagagttt tgacaaatga
     2521 agatatcaga gaattcttca cagaaccagc agaagcaaaa gaatttgcag aaagaacaaa
     2581 atgtgatgcc ttagcagtat gcttcggaac aatgcacgga atctatgctg aaccaccagt
     2641 acttgatatt gacagagtca aagaacttcg agaagcaatc cctgatgata caagaatcgt
     2701 aatgcatggt gcatcaggag tagaatttga ccaggtacag aatgcgatca cagcaggatg
     2761 ctcaaaagtt aactactatt cttatatggc aaaagctaca acaaaatttg ttgcagacaa
     2821 agtagctgag acagatggta agattgcata tcatgaatta caggaagcag catatgaatt
     2881 catgaaagga tatgcaaaag acgtaatcaa agcgttcaaa aacggaaaat aagataaatc
     2941 ataaaaagga gaatatttca tgagtggtgt aaatttagcg gaagtactac tcgatgaaat
     3001 ggtaattctt gattgtgagc cttttaagga caaagaaaat atgtttgatg tgatgactgc
     3061 aaaattcgaa gaagcaggat tcgtgtcaga cgcaaaggcc tacaaagatg ctctggaata
     3121 cagagaatcc ctagggccaa catatatggg aaacttcgtt gcaatacctc atggaaaatg
     3181 caaagaagtt ctgaaaccag gaattggatt ttgtagatgt aagaagccat tcatttatga
     3241 gtcctcaggg gaatctggtg aagttaaata catctttgta ttggcaatat ctgaaaatca
     3301 agagaatgat taccatttaa gggtattagc aacattagcg ggaatgttag cacacaaaga
     3361 gtttttagaa ctgttagaga aggcagaaag ctatgatgat ctgattcatg gcataaaaca
     3421 attacaacta taaaagctac aggaggtgga aactatgaaa gtagtagcag taacatcatg
     3481 cgcaactgga atcgctcata cttatatggc agcggaagct attatgaaag tatgtaaaga
     3541 gagaggttac aagtgtaagg tcgagaagca gggggctctc ggactggaag acaagttaag
     3601 agatcgtgat attaaagaag cggatctgat cgtttttgca aatgacgtag ggcttacaaa
     3661 agcagaaaga ttcgaaccat acaaagacaa gatcttccag acaaaaccac acgaagtgat
     3721 caaagacccg aatattattt ttagcggaaa ataagaaaag agaaacctta aaaatatttt
     3781 atgaaaggac gggaaacaaa tgagagatgt aattgatgat atcaaagaag cgtttttgac
     3841 aggtgtttca tacatgctgc cattcgtagt tgcaggagga
//
LOCUS       DAOMUE010000193         3845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6694_length_3845_cov_2.961214, whole genome shotgun sequence.
ACCESSION   DAOMUE010000193 DAOMUE010000000
VERSION     DAOMUE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6694_length_3845_cov_2.961214"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLC71_08300"
     CDS             complement(<1..738)
                     /locus_tag="PLC71_08300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203254.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRM02679.1"
                     /translation="MADNRTGKITQVIGAVLDIKFGSDNLPEINDAIDIQTKDGGKLV
                     VEVAQHLGDDTVRCIAMGPTDGLVRGMEAQATGGPISVPVGEATLGRIFNVLGDPIDN
                     KPAPEGANRLPIHRKAPEFSEQSTETEVLETGIKVVDLLCPYQKGGKIGLFGGAGVGK
                     TVLIQELIRNIATEHGGYSVFTGVGERTREGNDLYTEMSESGVIDKTAMVFGQMNEPP
                     GARMRVGLTGLTIAEDFRDRGGKDVLLF"
     gene            complement(771..1631)
                     /gene="atpG"
                     /locus_tag="PLC71_08305"
     CDS             complement(771..1631)
                     /gene="atpG"
                     /locus_tag="PLC71_08305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRM02680.1"
                     /translation="MASIIDIKRRKASVESTGQITKAMKLVSTVKLQRAKQRADESKA
                     YFNKMYETISSILAKAGNINHPYLQENGSTKKGIITISSNRGLAGGYNNNIVKLIRDS
                     GMNKEDIMIYAVGHKAMDTLKHRGYEIVSDDSEMINEPVYDDARILGEKLLKAFEDGE
                     IGEIYLAYTDFKNTVVHEPKLVKLLPIENDQQANEEKSESKAPMNFEPEPEEALSLLI
                     PKYICSMIYGALVTSVASENGARMQAMDSATSNAEEMISNLELAYNRARQGAITQELT
                     EIISGAEALK"
     gene            complement(1643..3151)
                     /gene="atpA"
                     /locus_tag="PLC71_08310"
     CDS             complement(1643..3151)
                     /gene="atpA"
                     /locus_tag="PLC71_08310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRM02681.1"
                     /translation="MNLRPEEISSVIKEQIKQYSTKLETSDIGTVIQVADGIARIHGL
                     EKAMQGELLEFPGEVYGMVLNLEEDNVGAVLLGDRKNINEGDIVKTTGRVVEVPVGDQ
                     LTGRVVNSLGQPIDNKGPIETDKYRPIERVASGVISRKSVDTPIQTGIKAIDAMVPIG
                     RGQRELIIGDRQTGKTAIAIDTIINQKGQGVHCIYVAIGQKASTVANIVKTLEEFGAM
                     DYTTIVASTASELAPLQYIAPYAGCAIGEEWMERGEDVLVVYDDLSKHATAYRTLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARLSDELGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLETEMFNAGFRPAINAGLSVSRVGGSAQIKAMKKIAGPIRTDLAQYREL
                     AAFAQFGSELDDDTKERLDQGERIREILKQPQYQPLPVEKQVAIIYAAVKKHLLDLPV
                     DQILDFQKELFELIDTKYPEIFTSIAETKVMDDATEEKLIKAINEAKESFRK"
     gene            complement(3173..3718)
                     /gene="atpH"
                     /locus_tag="PLC71_08315"
     CDS             complement(3173..3718)
                     /gene="atpH"
                     /locus_tag="PLC71_08315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HRM02682.1"
                     /translation="MASRVDKIYGDAYISVKTDEDDLLEALEEAKAVKEILDSNTDFV
                     NFLNHPQITKEEKMEAAKNVFDGRVSDDMTGFLMIIIKKGRFKEFDGIFTYIISNIKR
                     QLGIGALHVTSAMPLSQEQKDKILKKVIASSEYQKLEVTYETDKDIIGGLILCMDDRI
                     VDNSIRTKLDSMGRYLSQIQL"
BASE COUNT          895 a          939 c          712 g         1299 t
ORIGIN      
        1 gaataataag acgtcttttc ctccacgatc acggaaatct tctgcaattg taagtcctgt
       61 aagaccaact ctcatacggg ctccaggtgg ttcgttcatc tgtccaaaga ccatggcggt
      121 cttgtcgata acacctgatt cactcatctc tgtgtataga tcatttcctt cacgagtacg
      181 ttctcctaca ccagtgaata cagaatatcc accatgctct gttgcgatat ttcggatcag
      241 ctcctggatc agtacggttt ttcctactcc cgcaccaccg aacagaccga tctttccacc
      301 cttctggtat gggcatagaa ggtcaacgac cttgatccct gtttccagaa cttctgtctc
      361 tgtagattgt tctgaaaatt ccggtgcttt tctgtggatt ggtaagcggt tggctccttc
      421 tggtgccggt ttattatcaa taggatctcc cagcacgtta aagattcgtc ctaaggttgc
      481 ctctcctaca ggaacggaga tcggtccgcc tgtagcctgt gcttccattc cacggactaa
      541 tccgtcggtt ggtcccattg cgatacatct tacggtgtca tcacccaggt gctgtgcaac
      601 ttcaacgact aatttcccgc cgtcttttgt ctgaatatcg atcgcatcat tgatctctgg
      661 tagattgtca ctgccaaatt tgatatccag aaccgcaccg atgacctgtg tgatctttcc
      721 tgtacgatta tctgccatgt tatttcctcc tgcttcttat atcgagtcca tcacttcagg
      781 gcttctgctc ctgaaatgat ctctgttaat tcttgtgtga tcgctccctg tcttgctcgg
      841 ttgtatgcaa gttctaagtt tgagatcatc tcttctgcat tgcttgtcgc agaatccatt
      901 gcctgcattc tggctccatt ttcacttgct acagatgtaa ctaaggctcc atagatcatg
      961 ctgcagatat actttggaat taataaagaa agtgcttctt ccggctctgg ctcaaaattc
     1021 attggagcct tgctttcaga tttctcttca tttgcctgct gatcattctc aattgggagt
     1081 aattttacaa gttttggttc atgaaccacg gtattcttaa aatctgtgta tgccagatag
     1141 atctcaccga tctctccatc ttcaaaagct tttagaagct tctctcctaa gattctggca
     1201 tcatcataga ctggctcgtt gatcatctct gaatcatcag aaacgatctc atacccacga
     1261 tgttttaacg tatccatcgc tttgtgtcct accgcataga tcatgatatc ttctttgttc
     1321 ataccgctgt cccggatcag tttcacaata ttgttattat atcctcctgc taatccacgg
     1381 ttggatgaaa tggtaatgat ccctttcttt gtggaaccat tttcctgtaa atacggatga
     1441 ttgatattac ctgctttggc aaggatcgag ctgatcgtct catacatctt attaaagtat
     1501 gcttttgact catctgcacg ctgcttggct ctctgtaact ttaccgtgga tacaagtttc
     1561 atcgcttttg tgatctgtcc ggtactttcg acgctggctt ttcgtctttt aatatcaatt
     1621 atcgatgcca tgacagatca ccttactttc taaatgattc ttttgcttca ttgatcgctt
     1681 tgatcagttt ctcttctgtc gcatcatcca tgactttcgt ctcggcaatg cttgtaaaga
     1741 tctctggata ttttgtatca atcagttcaa ataattcttt ctggaaatca aggatctgat
     1801 ctactggcag gtctaagaga tgtttcttga ctgctgcata aatgatcgca acctgtttct
     1861 caactggtaa tggctgatac tgtggctgtt ttaagatctc tctgattctc tcaccctgat
     1921 ctaatcgttc ttttgtatca tcgtcaagct ctgatccaaa ctgggcaaat gctgccagtt
     1981 ctcgatactg tgcaaggtct gtacggattg gtcctgcgat cttcttcatt gccttgatct
     2041 gtgcagatcc tccaactcgt gatacagaaa gtccggcatt gatcgctggt ctgaaacctg
     2101 cattaaacat ctctgtttcc agatagatct gtccatctgt gatggaaata acgtttgtag
     2161 gaatgtatgc agatacatca cctgcctgag tctcgatgat cggaagggct gttaatgatc
     2221 ccccacctaa ttcatcagat aatctggctg cacgctctaa aagtcttgaa tgtaaataga
     2281 atacatctcc aggataagct tcacgtccag gtggtctgcg aagaagtaag gaaagtgtac
     2341 ggtatgcagt tgcatgtttg cttaaatcgt catatacaac taatacgtct tctcctcttt
     2401 ccatccattc ttcaccgatc gcacatcctg cgtatggtgc gatatactga agcggtgcta
     2461 attcactggc tgtggatgct acgatcgttg tataatccat cgcaccaaac tcttctaatg
     2521 tcttaacgat attagcaact gtggatgctt tctggccgat tgccacataa atacagtgaa
     2581 caccctgtcc tttctgatta atgatcgtat cgatcgcgat cgctgtctta ccggtctgtc
     2641 tgtcaccgat gatcaactca cgctgtcctc ttccgatcgg taccatcgca tcgatcgctt
     2701 tgattcctgt ctgaatcgga gtatctacgg attttcttga gattactcct gacgcaactc
     2761 tttcaattgg acgatattta tctgtctcga tcggaccttt attgtcaatt ggctgtccta
     2821 aggagtttac gacacgtcct gtcagttgat ctccaaccgg cacttctacc acgcggcccg
     2881 ttgtcttgac aatatcgcct tcgttaatat tctttcggtc acctaagaga actgcaccta
     2941 cgttgtcctc ttctaagttt aataccattc cataaacttc ccctggaaat tcgagcagtt
     3001 ctccctgcat cgctttctct aatccatgga ttctggcgat tccatcggca acctgtatta
     3061 ccgttccaat atcagaggtt tcaagctttg ttgaatattg tttgatctgt tctttgatca
     3121 cagaactgat ctcttctggt cttaaattca tggggcttat cttcctcctg ttctatagtt
     3181 gtatttgtga cagatatctg cccatcgaat caagctttgt acgaatggaa ttatccacaa
     3241 tccgatcatc catacaaagg atcaatccac cgatgatgtc tttatctgtc tcataagtca
     3301 cctctaactt ctgatattct gaagaagcaa tgactttctt taagatcttg tctttttgtt
     3361 cttgtgacag cggcattgct gacgttacat gtagggctcc gattcctaac tgacgtttga
     3421 tattcgaaat gatgtaagta aagattccat caaattcttt aaaacgtcct ttcttgatga
     3481 tgatcatcaa aaatcctgtc atatcatcag aaacccggcc atcaaaaacg ttctttgcag
     3541 cctccatctt ctcttctttt gtgatctggg gatgatttag gaaattcacg aaatctgtgt
     3601 tggaatctaa gatttctttg acagctttcg cctcttccaa agcttctaaa agatcgtcct
     3661 catctgtttt cacagaaata taggcatccc cgtaaatctt atctactctt gaagccatgt
     3721 cttatcaccc atttcctgta aggtctgatc aacgaaagca ttctggtctt tctcatccat
     3781 ctggttctgg atgaatttgc cagccattaa gactgccatc ttgaccatct cctgcttcat
     3841 ctgat
//
LOCUS       DAOMUE010000194         3819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6746_length_3819_cov_2.516738, whole genome shotgun sequence.
ACCESSION   DAOMUE010000194 DAOMUE010000000
VERSION     DAOMUE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6746_length_3819_cov_2.516738"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..717
                     /locus_tag="PLC71_08320"
     CDS             160..717
                     /locus_tag="PLC71_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRM02683.1"
                     /translation="MEQQTMKIQDLTLIALMAALTCILGPMSITLPFTPVPISFTNLV
                     IYFAVMVIGMKRGTISYLVYLLIGAVGLPVFSGFGGGLAKLAGPTGGYLVGFIFLALI
                     SGFFVEKFSGNIVMAVIGMVLGTAVTYTFGTIWLCAQMHLTFIQGLYAGVIPYLPGDA
                     AKIVIAIIVGSAVKKAVVKARVLPE"
     misc_binding    789..1044
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1150..1842
                     /gene="cysE"
                     /locus_tag="PLC71_08325"
     CDS             1150..1842
                     /gene="cysE"
                     /locus_tag="PLC71_08325"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRM02684.1"
                     /translation="MGFREFAKSQYDTIKLRDPALREEKEVFLYPYVKALYWHRVAHE
                     LYKKGEFYKARKISQKWARKTGIEIHPGAQIGEGFFIDHGHGVVIGETTIIGNNVTLY
                     QGVTLGGTGNETGKRHPTIEDNVMISSGAKVLGSITIGKNSKIGAGSVVVSDVPPNST
                     VVGVPGKVVKQDGERVNRIQSIVLDQIDLPNPVDMDIEKLARENAELRQALETFIIHY
                     QNSEAIRREKEK"
     gene            1839..>3040
                     /gene="cysS"
                     /locus_tag="PLC71_08330"
     CDS             1839..>3040
                     /gene="cysS"
                     /locus_tag="PLC71_08330"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRM02685.1"
                     /translation="MKIYNTLTRQKEEFVPVHPGKVGMYVCGPTVYNYIHIGNARPMI
                     IFDTVRRYFEYKGYEVNYVSNFTDVDDKIIKKANEEGVTATEIAERYIKECKQDMEAL
                     NIKPATHQPRATEEIGGMIKMIQTLIKKGHAYEVDGTVYFKTRSFKDYGKLSKKNIDD
                     LEAGHREIKVTGEEGKEDPLDFVLWKPKKEGEIAWDSPWGEGRPGWHIECSEMSKKYI
                     GDTIDIHAGGEDLIFPHHENEIAQSEACNGEKFANYWMHNGFLNINNKKMSKSAGNFF
                     TVREIGEKYPLQVIRFFMLSAHYRTPLNFSDTLVESAKTGLDRILTAIDLCREMAAKE
                     ETGSLSKEETEHFANIEVLVKKFEDAMEDDFNTADAVSAIFEIVRESNSTVKDFSADY
                     AKKVLKVL"
     assembly_gap    3041..3055
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3056..3252
                     /locus_tag="PLC71_08335"
     CDS             <3056..3252
                     /locus_tag="PLC71_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRM02686.1"
                     /translation="CSVLGIETTKEEEILDEEIEKLIEERQAARKNKDFARADEIRDQ
                     LLEQGIVLKDTREGVKWSRA"
     gene            3237..3692
                     /locus_tag="PLC71_08340"
     CDS             3237..3692
                     /locus_tag="PLC71_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HRM02687.1"
                     /translation="MEQSLIQKIKEGLYLDGLDPKSYSPLSLAYIGDAIYEIVIRTIV
                     MSAGNMSVNKYHKKSSSMVKASAQKEVFEKIEPFLTEEEMAVYKRGRNSKSGSVAKNA
                     SMMDYRKATGVEALVGYLYLAGDMDRIIELIGIGFDLNKKEEIGEEHES"
     gene            3682..>3819
                     /locus_tag="PLC71_08345"
     CDS             3682..>3819
                     /locus_tag="PLC71_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HRM02688.1"
                     /translation="MNHKEDLIAGRNAVIEALRAKKPIDKIFVLDGCQDGPVRTIARE
                     AR"
BASE COUNT         1356 a          541 c          900 g         1007 t
ORIGIN      
        1 aagattaaga aaggcatcgg tggtgccttt ctttttttag aatgatcaaa agaccccttt
       61 attgttaact tttaaaaaaa caaagttgac aataagggat tgatgatata taatggcagt
      121 cagtacagga aaataataaa agtgagggag aaatttatta tggaacaaca aacaatgaaa
      181 attcaggatt taacactgat tgcattaatg gcagcattaa cttgtatttt aggaccaatg
      241 tcgatcacat tgccatttac accagtaccg atttcattta cgaacttggt gatttatttt
      301 gcagtaatgg ttattgggat gaaacgtgga acgatcagtt atcttgtata ccttctgatc
      361 ggagcagtag gacttccagt gttttcaggg tttggcggag gacttgccaa acttgcagga
      421 ccaacaggag gatatttggt tggatttatc tttttggcat tgatcagtgg attctttgta
      481 gaaaaattct cagggaacat agtgatggca gtaattggta tggtacttgg aacagcagta
      541 acatatacat ttggaacgat ctggctttgt gcgcagatgc atttaacatt cattcaggga
      601 ttatatgcag gtgtgattcc atatcttcca ggagatgcgg caaagatcgt gatcgcgatc
      661 attgtaggaa gtgcagtgaa gaaggctgtt gtgaaggcga gagttttacc agaataaata
      721 acaaatactt tgtatgaaaa ataagaatct gttgacattt tgtatgaaaa agcataaact
      781 attcgtagtt aaatgcaatg aacagaatta gtacagacaa gttggaactg tcagagagaa
      841 gctgccatcg gctggaagcg gctttgaggg aagatgtctg gaatgcatct gggagcagac
      901 aagatgaata aagtagtttt gtacgttgcc ggcgttaacg gattcgagtg aaagtatcct
      961 gatcaagtga tattagtcag atatataata agaagatcag gaacttttaa aagagtggaa
     1021 ccgcggatat actccgtctc tttatttaga gacggagttt tttgtattat agaaaatttt
     1081 gatggaatcc tctatgatat aaaaagtcct gtaaattaca tactatatat tggaggttta
     1141 gaaattatga tgggttttcg agaatttgcg aaaagccaat atgatacgat caaactaaga
     1201 gaccctgcgt taagggaaga gaaagaagtt tttttgtatc cttatgtaaa agcactgtat
     1261 tggcatcgtg ttgcacacga attatataag aaaggagagt tttacaaagc tagaaagatt
     1321 tctcagaaat gggcgagaaa aactggaatt gagattcatc caggtgcaca gatcggagaa
     1381 gggttcttta ttgaccatgg gcatggagtg gtcattggcg agacgacgat catcggaaat
     1441 aatgtgacct tatatcaagg tgttacatta ggtggaactg gaaatgaaac tggaaaacgt
     1501 catccaacga tcgaagataa tgttatgatc agttcaggtg ctaaggtttt gggatctatt
     1561 acaattggaa agaactcaaa gattggtgca ggaagtgttg ttgtatctga tgtaccgccg
     1621 aattctactg ttgtcggagt tccaggtaag gtagttaaac aggatggaga gagagtcaat
     1681 cgcattcaga gtattgtgct tgaccagatc gatcttccaa atccagttga tatggatatc
     1741 gaaaagctcg caagagagaa tgctgaatta cgacaggcac tggaaacatt tattatacat
     1801 tatcaaaatt cagaagcaat cagaagggaa aaagaaaaat gaaaatctac aatacactga
     1861 caagacaaaa agaagaattt gtaccggtgc atccagggaa agttggaatg tatgtttgtg
     1921 ggccaacggt gtataactac attcatattg gaaatgcaag accaatgatc atttttgata
     1981 cggtgcgtcg ttactttgaa tataaaggat atgaagtaaa ttatgtttca aacttcacag
     2041 atgtcgatga caagatcatt aagaaggcaa atgaagaagg tgtaacagca acagagatcg
     2101 cagaaagata tattaaggaa tgcaaacaag atatggaagc gttaaatatc aaacctgcaa
     2161 cccaccagcc aagagcgaca gaagaaatcg gtggaatgat caaaatgatt cagacattga
     2221 tcaaaaaagg gcatgcatat gaagtagatg gaactgtata ctttaagaca agatctttta
     2281 aagattatgg aaaactctcc aagaaaaata ttgatgatct agaagcagga catcgcgaga
     2341 tcaaggtaac aggagaagaa ggaaaggaag atcctcttga ttttgtatta tggaaaccaa
     2401 agaaagaagg agaaatcgca tgggattctc catggggaga aggaagacca ggatggcata
     2461 ttgagtgttc tgagatgtct aagaaatata tcggagatac aattgatatt catgcaggtg
     2521 gagaagactt gatcttccct catcatgaaa atgagatcgc acagagtgaa gcatgcaatg
     2581 gggagaagtt tgcgaattac tggatgcata atggtttctt aaatatcaac aataagaaaa
     2641 tgtctaaatc agcgggaaac ttctttacag tgcgtgaaat tggagaaaag tatccattac
     2701 aggtgattcg tttcttcatg ttaagtgcac actacagaac cccattaaac ttcagtgata
     2761 ctttagtaga aagtgcaaaa acaggattag accgtatctt gacagcaatt gatctatgca
     2821 gagagatggc agctaaagaa gaaacaggtt ctttaagcaa agaagaaaca gaacattttg
     2881 ctaatataga agtacttgta aagaaatttg aagatgcaat ggaagatgac tttaacacag
     2941 cagatgcagt ttcggctatt tttgaaatcg tgcgtgaatc aaactcaaca gtgaaagact
     3001 tcagtgcaga ctatgcaaag aaagtactaa aagtattaga nnnnnnnnnn nnnnntttgc
     3061 agcgttcttg gaattgaaac aacaaaagaa gaagagatcc tagatgaaga aatcgaaaaa
     3121 ctgatcgaag aacgtcaggc ggcaagaaag aacaaagatt ttgcaagagc agacgagatc
     3181 agagaccagt tattagagca gggtatcgta ttaaaagata caagagaagg tgtaaaatgg
     3241 agcagagctt aattcaaaag atcaaagaag gattgtatct tgatggattg gatcccaaaa
     3301 gttactctcc attatctctt gcttacattg gagatgcgat ctatgagatc gtgatccgaa
     3361 cgatcgttat gtctgcaggg aatatgagtg tgaataagta tcacaaaaaa tcaagcagta
     3421 tggtcaaagc ttctgcacaa aaagaagtgt ttgaaaagat cgagccattt ttgacagaag
     3481 aagaaatggc tgtttataaa cgtggaagaa attccaaatc aggttctgtt gcaaagaatg
     3541 catctatgat ggattacaga aaagcaactg gagtggaagc tttggttgga tatttatatc
     3601 tggcaggaga tatggaccgc atcatcgaat tgatcgggat cggttttgat ttgaataaaa
     3661 aagaagaaat aggagaagaa catgaatcat aaagaagatc tgatcgcagg gcgtaatgcc
     3721 gtgatcgagg ctttaagagc aaagaaacca atcgacaaga tttttgtatt ggatggatgt
     3781 caggatggac cggtccgcac tattgccaga gaggcaaga
//
LOCUS       DAOMUE010000195         3813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6764_length_3813_cov_2.444918, whole genome shotgun sequence.
ACCESSION   DAOMUE010000195 DAOMUE010000000
VERSION     DAOMUE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6764_length_3813_cov_2.444918"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..539
                     /gene="mraZ"
                     /locus_tag="PLC71_08350"
     CDS             108..539
                     /gene="mraZ"
                     /locus_tag="PLC71_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRM02689.1"
                     /translation="MFMGEFNHTIDAKGRLIIPSRFREELGQEFVMTKGLDGCLFVFP
                     QNEWESFQGKLKTLPLINKDARKFSRFFMAGAAPCEMDKQGRTLIPATLREFAQMKKE
                     VVLTGMADRIEIWSKEKWIENNSYEDMDDIAASMQELGLSI"
     gene            552..1490
                     /gene="rsmH"
                     /locus_tag="PLC71_08355"
     CDS             552..1490
                     /gene="rsmH"
                     /locus_tag="PLC71_08355"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRM02690.1"
                     /translation="MEFKHISVLLDETIEELHIKPDGIYVDGTLGGGGHAYHVCQKLS
                     EKGRYIGIDQDADAIAASTKRLEPFKDKVTIVRDNYVNMPKVLHDLGIEHVDGILLDL
                     GVSSFQLDEKERGFTYREDVPLDMRMDQRQSLTARDIVNEYSEMELFHIIRDYGEEKF
                     AKNIAKHIVNKRKESPIETTGQLIEVIKGAIPMKVRAVGGHPAKKTFQAIRIECNREL
                     DVLKDSIDAMIDLLNPGGRLCIITFHSLEDRIVKRAFKKNENPCTCPPNFPVCVCGNV
                     SKGKQLTRKPIIPGEEELEYNKRAKSSKLRVFERAE"
     gene            1503..1997
                     /locus_tag="PLC71_08360"
     CDS             1503..1997
                     /locus_tag="PLC71_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRM02691.1"
                     /translation="MDKTRQERYNMIYGNTARKIEDLAAEPVRRQPRKTRKARPRREH
                     QVKESTRQNQKRAMSIDLAFIKVLVAGLCVIAVASFFYLSERTKVLNQKAALTDAKTQ
                     LNTLQVENEDLNLEIEQSIRFDTVETAAKKYGMVKPDSKHTLRYESQDAEYIRQYGKI
                     PKAD"
     gene            2010..>3241
                     /locus_tag="PLC71_08365"
     CDS             2010..>3241
                     /locus_tag="PLC71_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRM02692.1"
                     /translation="MKKRRKPIKRLNDAMKRRTMIACGIIALLFVLLIGRLIYFSIFK
                     NGIYKRQVLSQQNYSSETLPYKRGDILDRDGNTLATSQKMYTLVLEPKNILRSEEVQK
                     ATIDALHKYMKLDEDKLLKFIKRHKDSYYSVYREELSYSQIADLKDFIASDKAENVSG
                     IVFNEKYKRRYPNQSLASQVIGFVSDGTIGTGGIEQYYNSTLSGVDGRKYKYLNEELE
                     QDSSIVEPENGKTVVTTIDSNIQKLAEDQLSKFEKKYGSKGSSILVMNPNNGEIYAMA
                     NSTSYNLESPRDDKNLLKKYSQSQVNKMSEKEKTKAFNEIWKNPIVSNAFEPGSTYKP
                     FTVAAGLEEGILKGNETYYCDGYQKVGPHTIHCSHRSGHGLITLSQSISLSCNDALMQ
                     IAAKEGKNVFARYQKNFN"
     assembly_gap    3242..3251
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3252..>3813
                     /locus_tag="PLC71_08370"
     CDS             <3252..>3813
                     /locus_tag="PLC71_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRM02693.1"
                     /translation="TGIDLPGEAQTASLLHDAKDMTAADLATSSFGQSFNCSMIQMGS
                     AFCSLINGGNYYKPHVVKQLRSSNGEVVSNIEPTLVKQTISKETSDKLRKYMKETVDS
                     GTGTKAKMKDYTAGGKTGTAQKIPRSAGTYIVSFCGFAPVENPQVLVYVVIDEIQRDS
                     QTNTGLAVEIAKKVLEGSLKELNVAKS"
BASE COUNT         1354 a          601 c          901 g          947 t
ORIGIN      
        1 taagaaaaaa ctaagatttg attgtggatt attccttgca atttataagt ctatcgtata
       61 aaatgaaatt tataagtggt aagaagtggt ggaaagtggt gatgtccatg tttatggggg
      121 aatttaatca cacgattgat gcaaaaggca ggctgatcat tccttcacga ttccgggaag
      181 aattaggtca ggaatttgtg atgacaaagg gattggatgg atgcctgttt gtgtttccac
      241 aaaacgaatg ggaaagcttt caaggaaagc taaaaacttt gccacttatt aataaagatg
      301 ccagaaagtt ttcgagattt ttcatggcag gggcagctcc atgtgagatg gacaagcagg
      361 gaagaacctt gatcccagcg acacttcggg aatttgccca gatgaagaaa gaagttgtat
      421 taacaggaat ggctgaccgc atcgagatct ggagtaagga gaagtggatt gagaacaatt
      481 cttatgagga tatggatgat atcgcagcca gcatgcagga attaggatta agcatataag
      541 ttaggtggat tatggaattt aaacacattt ccgttttatt agacgaaacg atcgaagaac
      601 tacatattaa accagacgga atctatgtgg atgggactct gggcggaggc ggacatgcat
      661 atcatgtatg ccagaaacta tcagagaaag gaagatacat cgggatcgac caggatgcgg
      721 atgcgattgc tgcaagcacg aagcggttag aaccgtttaa ggacaaagta acgatcgtgc
      781 gtgataatta tgtaaacatg ccgaaagtgt tacatgatct tggaatcgaa catgttgatg
      841 gaatcttgtt agatcttggg gtatcatcct ttcagttaga tgagaaggaa cgtggattta
      901 cttacagaga agatgttccg cttgatatga gaatggatca gagacagtca ctgactgcaa
      961 gagatattgt caatgaatat tccgagatgg aactattcca tataatcaga gactacggcg
     1021 aagagaaatt tgcaaagaac attgcaaaac atatcgtgaa taagagaaaa gaaagtccga
     1081 tcgaaacgac cggacagtta atagaagtca tcaaaggtgc gattccgatg aaagtcagag
     1141 ccgttggagg acatccggcg aagaagacat ttcaggcgat ccgtatcgaa tgtaacagag
     1201 aattagacgt attgaaagac agcattgatg cgatgatcga tcttttaaat ccaggtggaa
     1261 gattatgtat cattaccttc cattctttag aagaccgtat cgtcaaacga gcttttaaga
     1321 agaatgagaa tccttgtaca tgcccgccaa acttcccagt atgtgtatgt ggcaacgtat
     1381 cgaaaggaaa acagttaaca agaaaaccaa tcattccagg ggaagaagaa ctagagtaca
     1441 ataagagagc aaaaagttcc aagcttcgag tatttgaaag ggcagaataa gggagagtat
     1501 caatggacaa gacgagacaa gaacgatata acatgatcta tggcaacaca gcaaggaaga
     1561 tagaagatct tgctgcagag ccagtgcgca gacagccaag gaagacaaga aaggcaagac
     1621 caagaagaga acatcaggta aaggagtcaa caagacagaa tcagaagaga gcgatgtcga
     1681 tcgatctggc ctttatcaag gtattagttg cgggactttg tgtgatcgca gttgcaagtt
     1741 tcttttatct gtcagagcgt acgaaagttt tgaatcaaaa agcggctctt acggatgcaa
     1801 agacacagtt gaacacgctg caggtagaga atgaagatct gaatcttgag attgaacaga
     1861 gtattcgttt tgatactgta gaaactgcgg caaagaaata tggaatggtg aaaccagatt
     1921 ccaaacatac tcttcgttat gagagccagg atgcagaata catacgccag tacgggaaga
     1981 taccaaaagc agactaagag gtggcaaact tgaagaaaag aagaaaacct atcaaacgat
     2041 taaatgatgc tatgaaacga cgtacgatga tcgcgtgcgg aatcatagca cttctttttg
     2101 ttctactgat tggaagattg atctatttta gtatatttaa gaacggaatt tacaaaagac
     2161 aggtattatc acagcagaat tattctagtg aaacgcttcc gtataaaagg ggagatatct
     2221 tagatcgtga tggaaataca ttagcaacca gccagaagat gtatacattg gttttagaac
     2281 caaagaatat tttaagatcc gaagaggtac agaaagcaac cattgatgca cttcataaat
     2341 atatgaagtt agatgaagat aagctgttaa aatttataaa aagacataaa gattcttact
     2401 attctgtata tcgagaagaa ctttcttata gtcagatcgc agatctgaaa gattttatcg
     2461 cttcagacaa agcagagaat gtctcaggga tcgtatttaa tgaaaaatac aaacgtcgtt
     2521 atccgaatca gtcactggca agccaggtca ttggatttgt atcagacggt acgatcggaa
     2581 ctggagggat tgagcagtat tataacagta ctttatcagg cgtcgatgga agaaaatata
     2641 aatatttaaa cgaagaatta gaacaggata gctccattgt ggagccagag aatgggaaaa
     2701 cagttgtcac aacgattgac tccaatattc agaaactggc agaagatcag ctttctaagt
     2761 ttgaaaagaa atatggaagt aagggaagca gcattcttgt gatgaatccc aataatggag
     2821 agatctatgc aatggcaaat tctacttcct ataatctgga aagcccaaga gatgataaga
     2881 atctcttaaa gaaatactca caaagtcaag ttaataagat gagtgagaaa gaaaagacaa
     2941 aagcatttaa tgaaatctgg aagaatccaa ttgtatcaaa tgcatttgaa ccaggttcaa
     3001 catataagcc atttacggta gcagctggtc ttgaagaagg aatcttaaag ggcaatgaaa
     3061 cttattattg tgatggatac cagaaggttg ggccgcatac tattcactgt tctcatagaa
     3121 gcggacatgg attgatcacg ttgtctcaga gtatttcatt atcgtgcaat gatgcattga
     3181 tgcagattgc agccaaagaa ggcaagaatg tattcgcaag atatcagaag aatttcaatt
     3241 tnnnnnnnnn ngacagggat cgatcttcct ggagaagcac agacagcatc gcttcttcat
     3301 gatgcaaaag atatgacagc cgcagatctt gccacaagtt cttttggaca gtcatttaac
     3361 tgctctatga tccagatggg aagtgcattt tgttctctga tcaatggagg aaattattac
     3421 aaaccgcatg tggtcaaaca acttcgaagc agtaatggag aagttgtcag caatatagag
     3481 ccgacgttag tgaaacagac gatctcaaaa gagacatcgg ataaattaag aaaatacatg
     3541 aaagaaactg ttgattcagg aactggaaca aaggcaaaga tgaaagatta tacagctggt
     3601 ggtaagactg gtactgcaca gaagattcca agaagtgcag gaacttatat cgtatctttt
     3661 tgtggatttg ctccagtgga gaatccacag gtgctagttt atgttgtaat cgatgagatt
     3721 cagagggatt ctcagacgaa tacaggactt gcagttgaga tcgcaaagaa ggtattagaa
     3781 ggatcactca aggaattaaa tgttgcgaaa agc
//
LOCUS       DAOMUE010000196         3789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6817_length_3789_cov_2.566952, whole genome shotgun sequence.
ACCESSION   DAOMUE010000196 DAOMUE010000000
VERSION     DAOMUE010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6817_length_3789_cov_2.566952"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PLC71_08375"
     CDS             <1..1195
                     /locus_tag="PLC71_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HRM02694.1"
                     /translation="LHKVTPTWLDNLTTPLISIMVTSFLTFIFVGPVLREAGNLLADG
                     ITWVYNTLGFVGGGLFGLAYAPICLTGMHHSFIAIETQLIAAKATTGGSFIFTTASMN
                     NVAQGAAVLAVLFLTKNEKMKSICSASGVSALLGITEPAMFGVTLKLKYPFYAAIIGS
                     AVGNAYCAATGVLAQALGAAGLPGFLSMLPKDYLNFVIGLLLSMGVSAVLTAVFWKKF
                     NVEGEDHKAETPVKEEVKEIEVQEPADTTEETNELYAPMKGEVLDVAKSADPAFASKA
                     MGEGVAINPSEGIVYAPADGTISLIFPTKHAMGITLNSGVELLIHAGIDTVKMNGEGF
                     ETFVETGAKVKKGDKLLSFDMDLVKEKGYKTQTMFLVADAKGKEVEVVEAADADNNTK
                     IMNLK"
     gene            1230..2696
                     /locus_tag="PLC71_08380"
     CDS             1230..2696
                     /locus_tag="PLC71_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HRM02695.1"
                     /translation="MFEKYKKVYKADYEKWYKENEAYRERVAADQDRLKFHLMPKTGW
                     LNDPNGLCQFNGTYHIYYQYTPFEPTGELKTWGHYTTKDFIHYEDFGPVVFPDEDIDA
                     HGVYSGSAFIEDDTIHYFYTGNLKYFDRDDYDYINSGRGSNTITFTSKDGYEFTKKEL
                     LMTTDDYPADMSNHVRDPKIFKKNEKYYMVLGARDVEGVGMILLYESTDLKNWTYKNR
                     ITTPQKFGYMWECPDLFEMDGQLYIICCPQGVETQGIDYENVHQVTAMKLDYDFDTDE
                     YEITDIKLFDRGFDFYAPQSFEDESGRRILIGWMGIPDADYTNPTEEAGWQHALTIPR
                     KLSVRDGKLIQEPIEELKQLRSEDKAVCTFCYGQTIIMQNAIYEAVVDFKRCREMVMT
                     LREGITLSYKDNILTLNLGVYGSGRSTRKVRLEKLEHLQIFADTSSLEIFVNHGEEVF
                     TTRIYNHYGRLLINGECSGIMTVYPLKSFEIEGGRHEE"
     gene            2686..3660
                     /locus_tag="PLC71_08385"
     CDS             2686..3660
                     /locus_tag="PLC71_08385"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HRM02696.1"
                     /translation="MKNKLCGIGEALIDFIPEVKGQRLKDVPSFKRVAGGAPANVVGA
                     VTKLGIPSKFLTKLGDDPFGDYIVEVLDEAGIDTSNIARDKEGETALAFVSLASDGNR
                     DFKFYRKNSADLRYSVEDIPADILNDCGMIHFCSVDLVESPMKEAHKKLIDMAIAQNV
                     KVSFDPNLRFSLWDDLDQLKETVNDFLKYADIIKISDEELEFITGHTDIKDALDGLFA
                     DRAKYVIYTKGADGAEVYTRNGMVEASGYKIDVRDTTGAGDSFIGAFLYCLLNDEVED
                     LESVSDDTLRNYLDFANAYAANTTTKEGALAAMADRAEMDEWMKTLKK"
BASE COUNT         1338 a          628 c          841 g          982 t
ORIGIN      
        1 gttacataaa gtaacaccaa catggttaga taacttaaca acgccattga tctctatcat
       61 ggtaacatca tttttaacat ttatttttgt aggcccagtt ttaagagaag caggtaactt
      121 attagcagat ggaatcacat gggtatataa cacacttgga tttgtcggtg gcggattatt
      181 cggacttgca tatgcaccga tctgtctaac aggaatgcat cacagcttta tcgcgatcga
      241 gacacagctg atcgcagcca aagcaacaac aggtggaagt tttatcttca caacagcaag
      301 tatgaacaac gtagcacagg gagcagccgt attagcagtt ttattcttaa caaagaatga
      361 gaagatgaaa tcaatctgtt ctgcatcagg tgtttctgca ttacttggaa tcacagaacc
      421 agcaatgttc ggtgtaacac tgaaattaaa atatccattc tacgctgcca tcatcggttc
      481 agccgtaggt aatgcatatt gtgcagcaac aggagttttg gcacaggcat taggagcagc
      541 aggacttccg ggattcctgt ccatgcttcc aaaagattac ttaaacttcg tgatcggact
      601 gctcttatcc atgggagtat ccgcagtttt aactgcagtc ttctggaaga agtttaacgt
      661 cgaaggagaa gatcacaaag cagagactcc ggttaaagaa gaagtaaaag aaatcgaagt
      721 acaggaacca gcagatacaa cagaagaaac aaatgaatta tatgcaccaa tgaaaggtga
      781 agttttagat gtagcaaagt ccgcagaccc agcatttgca agtaaggcaa tgggagaagg
      841 tgtagcgatc aacccaagcg aaggcattgt atatgcacca gccgatggaa cgatcagtct
      901 gatcttccct acaaaacatg caatggggat cacattaaac tcaggagtag aacttctgat
      961 ccatgcagga atcgatacag ttaagatgaa tggcgaaggt tttgagactt tcgtagaaac
     1021 aggagccaaa gtaaagaaag gtgataaatt attatctttt gacatggatc ttgtgaaaga
     1081 aaaaggatac aagacacaga cgatgttctt agttgcagat gcgaaaggaa aagaagtcga
     1141 agtggttgaa gcagcggatg cagataataa tacgaagatc atgaatttaa aataatcatt
     1201 attcgaagaa ggaaaggaaa ccagggcaga tgtttgaaaa atacaagaaa gtctataagg
     1261 cagattatga aaaatggtat aaagaaaatg aagcttatag agaacgtgtg gcagcagatc
     1321 aagaccgatt aaaattccat ttaatgccaa agacaggatg gttaaatgat ccaaatggtt
     1381 tatgccagtt taatgggaca taccatattt actaccagta cactccattt gaaccaactg
     1441 gtgagttaaa gacatgggga cattatacga caaaagattt tattcattat gaagattttg
     1501 gaccagtcgt attcccagat gaagacattg atgcccatgg tgtctattcc ggctctgcat
     1561 ttattgaaga tgatacgatc cactatttct acacaggaaa tttaaaatat tttgacagag
     1621 atgattatga ctatatcaac agtggacgtg gaagtaatac gatcacattt acaagtaaag
     1681 acggctatga gtttaccaaa aaggaattgt taatgacaac cgacgattat ccggctgaca
     1741 tgagcaacca tgtgcgtgat ccaaaaatct ttaagaaaaa tgagaaatat tacatggttc
     1801 ttggagcaag ggatgtcgaa ggtgtgggta tgattcttct atatgaatct acagatctta
     1861 agaactggac atacaagaac cgcatcacaa ctccacagaa gtttggttat atgtgggaat
     1921 gtccggatct gtttgagatg gacggacagt tatacatcat ctgctgccca cagggagtag
     1981 aaacacaagg cattgattac gaaaatgttc atcaggtcac agcaatgaaa ttagactatg
     2041 attttgatac cgatgaatat gaaatcacag atatcaaact ttttgaccgc ggatttgatt
     2101 tctatgcacc acaaagtttt gaggatgaaa gtggaagaag aattctgatc ggatggatgg
     2161 gaatcccaga cgcagactat acgaatccaa ccgaagaagc aggatggcag catgcactga
     2221 cgattccaag aaaattatcc gtaagagacg gaaaactgat tcaggaaccg atcgaagaat
     2281 taaaacaact tcgaagcgag gacaaagcag tttgtacatt ttgttatgga caaacgatca
     2341 tcatgcaaaa tgcaatctat gaagctgttg tagattttaa acgatgcaga gaaatggtaa
     2401 tgacactgcg cgaaggaatc actctttcct ataaagacaa tatcctgacc ttaaatcttg
     2461 gggtctatgg aagtggaaga tcaacaagaa aagtcagatt agaaaaatta gagcatcttc
     2521 agatttttgc ggatacttca tccttagaaa tctttgtgaa tcatggagaa gaagttttta
     2581 caacaagaat ctacaatcat tatggaagac tactgattaa tggagaatgt tctggtataa
     2641 tgactgtata tccattaaaa tcatttgaaa tcgaaggagg aagacatgaa gaataaatta
     2701 tgtgggatcg gagaagcact gatcgatttt attccagaag ttaagggaca gagactaaaa
     2761 gatgtacctt catttaaaag agttgcggga ggagcacctg ccaatgtagt cggggcagtt
     2821 acgaagcttg gtattccatc caaattttta acaaagcttg gagatgatcc atttggagat
     2881 tatatcgtgg aagtattaga tgaagcagga atcgacactt ctaatatcgc aagagacaaa
     2941 gaaggggaaa cagccttagc attcgtatcc cttgcctctg acggaaatcg tgatttcaaa
     3001 ttttatagaa aaaacagtgc agatttacgc tacagtgtag aagatatccc ggcagatatc
     3061 ttaaacgatt gtggaatgat tcatttttgc agtgtagacc ttgtggaatc tccaatgaag
     3121 gaagctcaca agaaactgat cgatatggcg atcgcgcaga atgtgaaagt atcttttgat
     3181 ccaaacttaa gattttcatt atgggacgat cttgatcaat taaaagagac agtcaatgat
     3241 ttcttaaaat acgcagatat catcaagatt tctgacgaag aactggaatt tatcacagga
     3301 catacagata tcaaagatgc tctggacggt ttatttgctg atcgtgcaaa atacgttatc
     3361 tacacaaaag gagctgacgg agcagaagtg tatacgagaa atggaatggt agaagcttct
     3421 ggatataaga tcgatgtccg agatacgaca ggtgctggag attcgtttat cggagcattc
     3481 ctatactgtc tgttaaatga tgaagtagaa gatttggagt ccgtaagtga tgacacatta
     3541 agaaactacc ttgattttgc caatgcttat gcagcgaaca caacaacaaa agaaggtgca
     3601 ttagcagcca tggcagatcg agctgagatg gatgaatgga tgaagacatt aaagaaataa
     3661 atcaaataaa tccttataga ccatagaaga aagaagagtt gaatgtagta tttggctctt
     3721 cttttttgta gtagaagaaa tctccgagat tcctaaatca gaaaaggaga tcgtagaacg
     3781 taattaaaa
//
LOCUS       DAOMUE010000197         3782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6836_length_3782_cov_2.161792, whole genome shotgun sequence.
ACCESSION   DAOMUE010000197 DAOMUE010000000
VERSION     DAOMUE010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6836_length_3782_cov_2.161792"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /gene="codY"
                     /locus_tag="PLC71_08390"
     CDS             complement(<1..684)
                     /gene="codY"
                     /locus_tag="PLC71_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HRM02697.1"
                     /translation="MSSIQLLDKTRKINKLLHNNNSQKVVFNDICKVLTEVMNSNVLV
                     ISKKGKILGKNEADHIEVLHQLISGNVKDYVDFSLNERLLTILSTNENVSLSTLGFES
                     DVDDYYAIIAPIDIAGERFGTLFMYRQRENYSIDDIILAEYGTTVVGLEMLRSVSEEV
                     EQERRKKGIVDSAISTLSFTEHKAVIHILEELEGNEGVLVASKIADRFGITRSVVVNA
                     LRKLESAGVV"
     gene            complement(700..>2738)
                     /gene="topA"
                     /locus_tag="PLC71_08395"
     CDS             complement(700..>2738)
                     /gene="topA"
                     /locus_tag="PLC71_08395"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRM02698.1"
                     /translation="YEVIASNGHVRDLPKSTLGIDIENDFEPKYITIRGKGEVLAALR
                     KAVKKADKVYLATDPDREGEAISWHLYYALKLENKNYSRITFNEITKTAVKDSIKHAR
                     DIDMNLVDAQQARRVLDRIVGYQISPILWAKIKRGLSAGRVQSVALRLICDREEEINL
                     FIPQEYWSLEALLDVKGSKKPLEAKLIGNKEQKIEIHSEEEMNEILSYLKGKEFTVEG
                     VKVTERLKKSPLPFTTSTLQQDASSKLNFAPQKTMRIAQQLYEGVNIKGQGTVGLITY
                     LRTDSTRISVEADAAAKEYIKEAYGAEFVGAGTVAKKDDKKVQDAHEAIRPTYMTNSP
                     ASIKDELSRDQFRLYQLIWNRFMASRMAPAKYENTSVKIAAGDYRFNASASKIAFDGF
                     LSVYNINNEKSEGNVMLKSIDEDTKLTLNNLEPKQHFTQPPAHYTEASLVKTLEEFGI
                     GRPSTYAPTISTITARRYVVKEKKNLYVTELGEAVNNMMKKAFASIVDVNFTAMMEGL
                     LDMVEEGKVHWKSVIENFYPDFEIAVKNAEKELEKIKIEDEVTDVICDECGRNMVVKY
                     GPYGKFLACPGFPECRNTKPHFEKVGIPCPMCGGEVVLKKTKKGRKYYGCENNPECEF
                     MSWQKPSTVKCEKCGSYMIEKGKKLVCSSKECGFVCSMPEDAKEKETSGESVSK"
     assembly_gap    2739..2752
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2833..>3782)
                     /gene="dprA"
                     /locus_tag="PLC71_08400"
     CDS             complement(2833..>3782)
                     /gene="dprA"
                     /locus_tag="PLC71_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRM02699.1"
                     /translation="MTQLEYDRFVHSRDETAIQKGYEALHRENIRFIIQKEAAYPNRL
                     RKIYDAPYGIFQKGQPYEEKELSIAIVGSRYPTTYGFEISKKFAKELAQQGVQIVSGL
                     ARGVDGTVHREIIDKKGQAVGVLGCGIDLIYPKENFQLFYDMYANQTVISEYPPGSEP
                     LKWHFPERNRIISGLSDGILVTEARARSGSLITADCGLDQNKEVFAVPGMITDKHCQG
                     CHGLIKQGAKLVENVDDILTEFSEFTGFSRKFPKHSAKSLAKAEKRVYDMLSLKPKYI
                     DEILSTSQDSYEEVIQALFQLEAKGYIKQIHQNLYIKKM"
BASE COUNT          882 a          829 c          687 g         1370 t
ORIGIN      
        1 aacaactccg gcactttcta gtttccttag tgcgtttacg acaacagatc ttgtgatccc
       61 gaatctatct gcgatcttac ttgcaactaa gactccttca ttaccttcta attcttctaa
      121 gatatggatc actgctttgt gttctgtgaa ggataatgta ctgatcgcgg aatcaacgat
      181 tcctttcttt cttcgttcct gttccacttc ttcgcttaca gatcttaaca tttcaagtcc
      241 gacaacggtt gttccatatt ccgctaagat aatatcatca atggaataat tttctctctg
      301 gcggtacata aataatgttc cgaatctctc tcctgcgata tcgatcggtg cgatgatcgc
      361 atagtaatca tcaacgtctg actcaaatcc cagtgtggaa aggctgacat tttcattggt
      421 ggaaagaatc gttaatagtc tttcatttaa tgaaaaatca acataatctt tgacatttcc
      481 tgagatcagc tgatgaagca cttcgatgtg gtctgcttca ttttttccta ggatctttcc
      541 ttttttactg atgacaagga cgtttgaatt cattacttct gtcagaactt tacagatatc
      601 gttaaataca actttctgtg aattgttatt atgaagtaat ttattgatct ttcttgtttt
      661 atctagtaat tgtatactac tcatctatat tttttctcct tattttgaca ctgactcccc
      721 agaagtctct ttctcttttg catcttctgg catgctgcat acgaatccac attccttgct
      781 agagcataca agcttcttac ctttttcgat catatagctt ccgcattttt cacatttgac
      841 tgtggatggt ttctgccatg acataaattc acattccgga ttattttcac atccgtaata
      901 ttttcttcct tttttcgttt tctttaagac aacttctccg ccgcacattg ggcatgggat
      961 tccgactttt tcaaaatgtg gttttgtatt tctgcattct gggaatccag ggcatgcaag
     1021 gaatttccca tatggtccgt acttcacgac catatttctt ccacattcat cgcagataac
     1081 gtctgtgact tcgtcttcga tcttgatctt ctctaattct ttttctgcat tcttcactgc
     1141 gatctcaaag tctgggtaga agttttcaat gacggatttc caatggactt taccttcttc
     1201 taccatatca agcagacctt ccatcatcgc tgtaaagttc acatcgacaa tacttgcaaa
     1261 tgctttcttc atcatgttgt tgactgcttc tccaagctcc gtgacgtata agttcttctt
     1321 ttctttgaca acataacgtc ttgctgtgat cgttgagatc gttggtgcgt aagtacttgg
     1381 tcgtccgatt ccaaactctt ctagtgtctt aactaaagat gcttctgtat aatgagctgg
     1441 tggctgtgta aaatgctgtt ttggttctaa attattcaat gtaagtttgg tatcttcatc
     1501 gatggatttt aacattacat ttccttcgga tttctcattg ttaatgttat atacagatag
     1561 gaatccatcg aaggcgatct tgcttgcgga tgcattaaaa cgataatctc cggcagcgat
     1621 ctttacagat gtattctcgt atttggctgg tgccatacgg cttgccatga aacggttcca
     1681 gatcagctgg tataaacgga actgatctct tgatagttca tctttgatgc ttgctggtga
     1741 gtttgtcatg taggttggtc ggatggcttc atgtgcatcc tgtactttct tgtcgtcttt
     1801 cttggcaaca gttcctgctc caacgaactc ggctccataa gcttccttga tatattcttt
     1861 cgctgcggca tcggcttcta cagagatacg tgtggagtct gtacgtaaat atgtgatcag
     1921 accgacggtt ccctgtcctt tgatattgac accttcatat aactgctgtg cgatacgcat
     1981 cgttttctgt ggtgcgaagt ttaacttgct ggatgcatcc tgctgaagag tacttgtcgt
     2041 aaatggaagt gggctcttct ttaatcgttc tgtgactttc actccttcga cggtaaattc
     2101 ttttcctttt aaataggata agatctcatt catctcttct tcgctgtgga tctcgatctt
     2161 ctgttcttta ttgccgatca gtttggcttc taatggcttt ttgcttcctt tgacatctaa
     2221 taaggcttct aaggaccagt attcctgtgg aataaacaga ttgatctctt cttctctgtc
     2281 acagatcagg cgtagagcta cggactgaac tcgtccagca cttaaacctc gtttgatctt
     2341 tgcccagagg atcggactga tctggtatcc tacgattcgg tccagaacac gtcttgcctg
     2401 ctgtgcatcg actaaattca tatcgatgtc tcttgcgtgt ttgatggagt ctttgacagc
     2461 tgtttttgtg atctcattaa atgtaattcg gctgtagttt ttgttctcca gttttaatgc
     2521 gtaatataaa tgccatgaga tggcttctcc ttcacggtcc gggtcagtcg cgaggtatac
     2581 tttgtctgct ttctttactg ctttacgaag agctgctaat acttcaccct tcccacggat
     2641 cgtaatgtac tttggttcaa agtcattctc gatatctatt cctaaggtac tctttggcag
     2701 atcgcgaaca tgcccgttgg acgcgatcac ttcgtaatnn nnnnnnnnnn nncagaaatt
     2761 ttttaattgt ttttgctttc gccggtgatt cgacgataac tagatatttt ggcatatagt
     2821 attacccctt gtttacattt ttttgatata taagttctga tggatctgtt taatatatcc
     2881 tttcgcttct aattgaaata aagcttggat cacttcttca taagaatctt gggatgtact
     2941 taagatctca tcaatatatt tcggtttcaa actcaacata tcatacaccc ttttttctgc
     3001 ctttgcaaga gattttgcac tatgctttgg aaattttcgt gaaaatcccg tgaattctga
     3061 aaattctgtt aaaatatcgt cgacattttc aactaatttt gcaccttgtt tgatcaatcc
     3121 atgacaccct tggcaatgtt tgtctgtgat cattccggga actgcgaaca cttctttgtt
     3181 ctgatcaagc ccgcaatccg ccgtgatcag cgatccgctc cttgctctgg cttctgtgac
     3241 gaggattcca tcacttaatc cactgatgat tcggttcctt tctggaaaat gccatttgag
     3301 cggttcactg cctggcgggt actcagaaat aactgtctga ttcgcataca tgtcataaaa
     3361 taactgaaaa ttttcttttg ggtaaatgag atcaatccca catcctaaaa ccccaacagc
     3421 ctgtcctttt ttgtcgatta tttcacggtg aaccgtccca tctacgcccc ttgcgagtcc
     3481 acttacaatc tgtacgcctt gctgagccag ctcttttgcg aacttttttg agatttcgaa
     3541 tccataagtt gtcggatatc ttgatccaac gatcgctatt gacagttctt tttcttcata
     3601 tggctgacct ttttgaaaaa tcccatacgg agcatcgtag atctttctta atcgattcgg
     3661 gtaagcagct tctttttgaa tgataaaacg gatattttca cgatgcaagg cttcatagcc
     3721 tttctgaatc gctgtttcat ctcttgaatg aacgaaacga tcgtattcaa gttgtgtcat
     3781 gt
//
LOCUS       DAOMUE010000198         3776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6850_length_3776_cov_2.136522, whole genome shotgun sequence.
ACCESSION   DAOMUE010000198 DAOMUE010000000
VERSION     DAOMUE010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6850_length_3776_cov_2.136522"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PLC71_08405"
     CDS             <1..867
                     /locus_tag="PLC71_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRM02700.1"
                     /translation="DIQKELFESSHRNEYVLERMKRISKRAVWAMEQHIKRGDFTPEE
                     YEIAFGEENHLDSMTFALEDGEKMFFSGVVDRMDSIEDDENKYLKIIDYKSGKQKFDF
                     AKIFHGLQMQLIIYMNAMMELYEKKTGKRVYPAGMFYFHMDDPIVNVEHENEAEDKIL
                     KDLKMSGVVNEDFQLIDHMEHTGSEGYLTLPVRATKNGYDKRSSVLNTTQLFNLGRIV
                     EKKMTELGNSLMHGDISIKPYEYEGRKPCEYCEFKNICAYEDGVDQVEKIKKVSLEEG
                     KHALDQTTAESH"
     gene            833..>3776
                     /gene="addA"
                     /locus_tag="PLC71_08410"
     CDS             833..>3776
                     /gene="addA"
                     /locus_tag="PLC71_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HRM02701.1"
                     /translation="MPWTKQQQKVIDQRDADILVSAAAGSGKTAVLVERIIQKITDEK
                     HPIDVDHLLVVTFTKAAAGEMKERIMAALDEKVREFPGNQHFVKQLSLIHKAQITTIH
                     SFCMNLIRDYFYVLGIDPNTAPGDEGRLSAIRKEILDDLLEEAYEKKEEDFINLIESY
                     SPGKNDNIISEYILKLYENARSHREPEKWLDQAEENISVETKEQFDQAPFMKIILRDA
                     RFSIDGAYDTIQEALELALSPGGPYFYEKTLRKDLEIIIKIKEAQTFSELCESFVKME
                     KPRLSGRKKKTDEIDEMLQDQCKYERNQAYNLLDDLKAAYFYENEAEIFHELRRIKSP
                     LKGLIDLTRKFMIRYDEKKKNENIMDFDDMEHFALELLIDHYDEEGNPVPSKIAREKS
                     DGYEEIYIDEYQDSNYIQDAILRSVSKESEGGHNMFMVGDVKQSIYSFRLARPELFLE
                     KYHGYQQKGEEYQLIELRNNFRSRSEVLTFVNDVFYQIMHEDLGNIEYTQNVALVPTM
                     EFEQGCDAQTELLLLESNEVKDSEEDAVVLEARMIATRIHEMIDGEDPMMVTGKDEEG
                     NMILRKAKYSDIVILLRSMKTNAEVIQKELMNAGIPAFANNQKGYFDTVEIRTLLSLL
                     SVVDNIYMDIDLAAVLRSPMIGMSEEELGRLKVDGEKDSLYECLCETKDKMEKSKKAL
                     ELLDLLRDAKTYLPLTQLIWLALEKSGYYHYAGAMPQGKKRQGNILMLVEQAKAFESS
                     QIKGLFHFVRFIEQCREYDMDYGEANTMSEDQDLVRISSIHKSKGLEYPIVFVSKIHQ
                     KFNLRDGNGSMIFHGDYFIGADHIDPVYRTRKKTILKNLIKNQMTRESLGEELRVLYV
                     AMTRAKEKLILTGIKKDDIDWNAKGEVTAIDRLSGRSYFDWIKKALPMIPKQDYRLKI
                     YTLEDLLWQQEGNELLHEMNRQIFYERLNDQIPNENVKALKESFAYHYRNEAETKGRL
                     KYSVSEIKR"
BASE COUNT         1353 a          534 c          893 g          996 t
ORIGIN      
        1 gatatccaaa aagaattgtt tgagagcagc cacagaaatg aatatgtcct ggagcggatg
       61 aaacgaatct ctaaacgagc ggtatgggcg atggaacaac atatcaagag gggtgatttt
      121 acaccagaag aatatgagat tgcctttgga gaggaaaatc atttagattc catgacgttt
      181 gctttagaag atggagagaa aatgttcttt tctggagtgg ttgaccgtat ggacagtata
      241 gaagatgatg aaaataaata tttaaagatc atcgattaca aatcaggtaa acagaagttt
      301 gattttgcca agatttttca tggattgcag atgcagctga tcatttatat gaacgcaatg
      361 atggaattat acgaaaagaa aacaggaaag cgggtatatc cggcaggaat gttttatttt
      421 catatggatg atccgatcgt caatgtagaa cacgagaatg aggcggaaga taagatttta
      481 aaagatctta agatgagtgg cgtcgtcaat gaagattttc agttgatcga tcatatggaa
      541 catacgggat cagaaggata tctgacactg ccggtccgtg caacgaagaa tggatatgac
      601 aagagatctt ctgttttaaa tacaacacag ttgttcaatc tgggaagaat tgtggaaaag
      661 aaaatgacag agcttggaaa ttcattgatg catggagata tttctataaa accatatgaa
      721 tatgaaggaa gaaaaccttg tgagtattgt gagtttaaga atatctgcgc ctatgaagat
      781 ggtgttgatc aggtagagaa gattaagaaa gtatcactag aggagggaaa acatgccttg
      841 gaccaaacaa cagcagaaag tcattgatca aagagatgcc gacatcttag tatctgctgc
      901 ggcaggttca ggaaagacgg cggtactggt agaacgtatt atacaaaaga tcacggacga
      961 aaaacatccg atcgatgtgg atcatctgct tgtcgtgaca tttacgaaag cagcagcagg
     1021 ggagatgaaa gaaagaatta tggcggcact tgatgaaaag gtgcgggaat ttccaggaaa
     1081 tcagcatttt gttaagcagc tgtcactgat tcacaaagca cagattacaa cgatccatag
     1141 tttttgtatg aatctgatcc gcgattattt ttatgtgctt ggtattgatc cgaatacagc
     1201 accgggggat gaaggaagat tatctgcaat aagaaaagaa atattggatg atctgctgga
     1261 agaagcttat gagaaaaaag aagaagattt tatcaatctg atcgaatctt attctccagg
     1321 aaaaaatgat aatatcatca gtgaatatat tttaaagtta tatgaaaatg caagaagcca
     1381 cagggaacca gaaaaatggc ttgatcaggc agaagaaaat atttcagtag agacaaaaga
     1441 acagtttgat caggcaccat ttatgaagat catcttaaga gatgcaagat tttcgataga
     1501 tggtgcatat gatacgattc aggaagcatt agaactggca ctttctcctg gtggaccata
     1561 tttttatgaa aaaacactgc gaaaagattt agagatcatc ataaagatca aagaagcaca
     1621 gacattttct gaattatgtg aaagctttgt aaagatggag aaaccacgac tttctggacg
     1681 taagaaaaaa acagatgaga ttgatgaaat gcttcaggat cagtgtaagt atgaaagaaa
     1741 tcaggcgtac aatctgcttg atgatctgaa agcagcatat ttttatgaaa atgaggcaga
     1801 aatttttcat gaattaagaa ggatcaaaag tccattaaaa ggattgatcg atctgacgag
     1861 aaaatttatg attcgttatg atgagaaaaa gaagaatgaa aatatcatgg atttcgatga
     1921 tatggaacat tttgcgctag aacttttgat cgatcattac gatgaggaag gcaatccagt
     1981 gccaagtaag atcgcaagag aaaagtccga tggatacgaa gaaatctata tcgatgaata
     2041 tcaagacagt aactatattc aggatgcgat cttaaggagt gtatccaaag aatccgaagg
     2101 cggtcataat atgtttatgg taggggatgt taagcagagt atttatagtt tccgactggc
     2161 aaggccagag ctgtttcttg aaaaatatca tggatatcag caaaaggggg aagaatatca
     2221 gctcattgag ctccgtaata atttccgaag ccgcagtgag gtccttacat ttgtcaatga
     2281 cgtgttttac cagatcatgc atgaagatct tggaaatatc gaatatactc aaaatgttgc
     2341 tcttgttcca acgatggaat ttgaacaggg atgtgatgca cagacagaac tgttactgtt
     2401 agaatctaat gaagtcaaag actcggaaga agatgcagtt gttttggaag ccagaatgat
     2461 cgcaacaagg attcatgaga tgatcgatgg agaagatcca atgatggtca ctggaaaaga
     2521 tgaagaaggc aatatgattt taagaaaagc caaatacagt gatatcgtta ttttgcttcg
     2581 ttctatgaag acaaatgcgg aagtgatcca gaaagaattg atgaatgcgg gaatcccagc
     2641 atttgcaaat aatcagaaag ggtattttga tacggtggag atccgcacgt tattaagttt
     2701 attgtctgta gttgataata tatatatgga tattgatctt gcagctgtct taaggtctcc
     2761 aatgatcggg atgtcagagg aagaactagg acgattaaag gtagatggag aaaaagattc
     2821 tctctatgaa tgtctttgtg agacgaaaga taagatggaa aaatcaaaga aagcactgga
     2881 attgcttgat ctgttacggg atgccaagac atatctgcca ttaacacagt tgatctggct
     2941 ggctctggaa aaaagtgggt attatcatta tgcaggagcc atgccacaag gaaagaaacg
     3001 acaaggaaat atcctgatgc tcgttgaaca ggcaaaagct tttgaaagca gccagattaa
     3061 gggattgttt cattttgtgc ggtttatcga acagtgcagg gaatacgata tggattatgg
     3121 agaggcaaat acgatgagcg aagatcagga tctggtccgt atttccagta tccataaaag
     3181 taagggattg gaatatccga ttgtttttgt atcaaagatc catcagaaat ttaatttaag
     3241 ggatgggaat ggaagtatga tctttcacgg agattatttt atcggagcag atcatattga
     3301 tcccgtttat cgtacaagaa agaagacgat cttaaagaat ctgatcaaaa atcagatgac
     3361 aagagagtct ttaggagaag aattaagagt tctttatgtt gcaatgacaa gagcaaaaga
     3421 aaaactgatc ttgactggaa tcaagaaaga tgatattgac tggaatgcaa aaggagaggt
     3481 cacagcaatt gatcgtttga gcggaaggag ttattttgac tggatcaaaa aggcacttcc
     3541 gatgattcca aaacaggatt atcgattgaa aatctatact ctggaagatc ttttgtggca
     3601 gcaggaagga aatgaacttc ttcatgagat gaaccgccag atcttctatg aacgtctaaa
     3661 tgatcagatt ccaaacgaaa atgtaaaagc gctgaaagag agttttgcat atcattaccg
     3721 caatgaggca gagacaaaag gtcgtttaaa atattctgta tcagagatca aacgta
//
LOCUS       DAOMUE010000199         3729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6973_length_3729_cov_2.218835, whole genome shotgun sequence.
ACCESSION   DAOMUE010000199 DAOMUE010000000
VERSION     DAOMUE010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6973_length_3729_cov_2.218835"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLC71_08415"
     CDS             complement(<1..188)
                     /locus_tag="PLC71_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HRM02702.1"
                     /translation="MEDKLYWYWLCGKVRLPIKKQKELMDIFIHPKELYKINPAILKE
                     HMTQLEYDRFVHSRDETA"
     gene            complement(178..1719)
                     /locus_tag="PLC71_08420"
     CDS             complement(178..1719)
                     /locus_tag="PLC71_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRM02703.1"
                     /translation="MSIIYQEVITGIVSGVSGKLIHVEVEIRNGLPYFTMVGYLSKES
                     MEAKERVASALRSIGHPLPSMKITVNLSPANIRKNGTAFDFPIAVAFLGCLNLIDASK
                     LDDICIMGELGLNGKIRGVGNCLPIVLEARKQGIHKFFAASEDENSLRGIEDIEVVFM
                     DSLQDVLDYFHGTYHQKSCRSATTYQKEESEEDFSDIIGQQHLKRAMEIAVTGRHHLL
                     IIGSPGSGKTMAARRIPTILPQLSKEEKMEVQAIYDATGIPRYLQDDTRPFRQPHPMI
                     PKAAFLGGGKTPTAGEITLAHHGILFLDEFMEFKTECIEALRQPLEDGTIDITRNGIY
                     HKFPADFQLIATMNPCPCGYGLEDGICRCTYHEKKRYLKKLSGPILDRFDMVLCLSKK
                     EADTQKIQKESQETSNQIKERIETTIQREKKLLKNYQCSDTSHLSHIQLNKLLHLSKE
                     CKEILDIAYRSGKITRRGMDKILKVALTIMLMENESEIKPIHLMEAMTFRNTGFIKEV
                     LEYGR"
     gene            complement(1820..2269)
                     /gene="nrdR"
                     /locus_tag="PLC71_08425"
     CDS             complement(1820..2269)
                     /gene="nrdR"
                     /locus_tag="PLC71_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRM02704.1"
                     /translation="MRCPYCGSENSRVVDSRPAEDNNAIRRRRQCDECGKRFTTYEKI
                     ETEPLSVIKKDKTRQLYDRNKIRNGIVQSCHKLPVSMGQIEAAIDEIETKIYNMGTRE
                     VETSVIGEMVMDKLKDLNEVAYVRFAAIYREFKDVNTFMSELKKLLK"
     gene            complement(2339..>2605)
                     /locus_tag="PLC71_08430"
     CDS             complement(2339..>2605)
                     /locus_tag="PLC71_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HRM02705.1"
                     /translation="RKRIPDMKFLELRQKDVINCNTGECLGFVLDLEFEIHTGKICYL
                     IVPKTTKMLICVGKNKVYKIPYKCIVRIGRDTILVDINEKECLK"
     assembly_gap    2606..2635
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2720..3556)
                     /locus_tag="PLC71_08435"
     CDS             complement(2720..3556)
                     /locus_tag="PLC71_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HRM02706.1"
                     /translation="MNISAILLAMVVVGGTGLLIAILLGIASEKFKVPVDEKEVAIRA
                     ELPGNNCGGCGYAGCDGLAKAIANGEAPVNGCPVGGAAAAEKISAIMGVEAGEFVKKV
                     AFVKCAGTCEKASDKYQYSGVQSCIEAMNVPGAGPKGCEFGCMGFGSCAAVCPENAIS
                     IVNGIAHIDEEACVGCGKCAETCPKALIDLVPENKKTRVQCSSKEFGKNVMSVCKAGC
                     IGCKACEKVCKLGAIKVENNIAQIDYDKCVGCGLCAGKCPKGIITGKKLVPKKPAPKK
                     EA"
     gene            complement(3569..>3729)
                     /locus_tag="PLC71_08440"
     CDS             complement(3569..>3729)
                     /locus_tag="PLC71_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HRM02707.1"
                     /translation="ISLGFLIAIVILAGIREKMEYNNIPESFKGMPITLLTAGLMAIA
                     FFGFSGLI"
BASE COUNT          893 a          821 c          675 g         1310 t
ORIGIN      
        1 atcgctgttt catctcttga atgaacgaaa cgatcgtatt caagttgtgt catgtgttct
       61 tttaagattg ctgggttgat cttatagagt tcttttggat gaatgaagat atccatcaat
      121 tctttttgct tcttgatcgg caatcggact ttgccgcata gccagtacca atataattta
      181 tcttccatat tcaagcacct ctttgataaa tcctgtattt ctgaaggtca tggcttccat
      241 cagatggatc ggtttgatct ctgattcatt ttccataagc atgatcgtca gggctacttt
      301 taaaatctta tccattcctc gccttgtgat cttgccagat cgataggcga tatctaaaat
      361 ctctttgcat tctttggata aatgaaggag cttgtttaac tgtatgtggc ttaaatggct
      421 ggtatcactg cattgatagt tttttaataa ctttttttct ctttggatgg ttgtttcgat
      481 gcgttctttg atctgattgg atgtttcttg actctctttt tgtatcttct gtgtgtcagc
      541 ctctttttta cttagacata ggaccatgtc aaaacggtct aagatcggac cggacaattt
      601 ctttagataa cgcttttttt catgataagt acatcggcag attccatctt ccaatccata
      661 accacaagga catggattca tggtggcgat cagttgaaag tctgccggaa acttgtggta
      721 gatcccattt ctggtgatat cgatcgttcc atcttccaat ggctgtctga gtgcttcgat
      781 acattctgtt ttaaattcca taaattcgtc cagaaataaa atgccatgat gtgctaatgt
      841 gatctctcct gccgtcggcg tcttgccgcc tccaaggaat gcggctttcg ggatcattgg
      901 atgtggctgg cgaaatggtc tggtatcatc ttgtaaatac cgtggaattc cagtggcatc
      961 atagattgcc tgaacttcca tcttctcttc ttttgatagt tgtgggagaa tggtcgggat
     1021 ccttctggct gccattgttt ttcctgaacc tggggaacca atgatcaata gatgatgtct
     1081 tccagtgacc gcgatctcca tcgctctttt taaatgttgt tgtccgatga tgtcactgaa
     1141 gtcttcttct gactcttcct tttgataagt tgttgcactt ctgcaacttt tttgatgata
     1201 tgttccatga aaatagtcca ggacatcctg taaactgtcc ataaatacca cttcaatatc
     1261 ttcgatccct cttagagaat tctcatcttc acttgccgca aagaacttgt gaattccttg
     1321 ttttcttgct tctaaaacga tcggaaggca gtttcctaca cctctgatct tgccgtttag
     1381 tcctaattct cccatgatac agatgtcatc taacttgctt gcatcgatta aattcaggca
     1441 tcctaaaaag gccacggcga ttgggaaatc gaacgcggtt ccgttctttc ttatattggc
     1501 tggggataaa ttaacggtga tcttcatcga cggtaaggga tggccgatgg agcgcagtgc
     1561 tgatgcaacc cgctcttttg cttccatgga ttctttggaa agatagccta ccattgtaaa
     1621 atatggcagg ccgtttctga tctctacttc tacgtggatg agctttccgc tcactccact
     1681 gacgattcct gtgattactt cctgataaat aatactcaac tccttctctt ctcaaaaagg
     1741 aagctggttt caaaatgata tggttagttt atcacaaaag taatattttt acaatcttgt
     1801 ttattcaaaa caattgtgat tattttaata atttctttaa ttcactcata aatgtgttca
     1861 catctttgaa ttcacggtaa atggctgcaa atcttacgta tgcaacttca tttaaatcct
     1921 ttaatttgtc catgaccatc tctccgatca cagatgtttc aacttctctt gttcccatgt
     1981 tatagatctt tgtctcgatc tcatcgatcg ctgcttcgat ctgccccatg gatactggga
     2041 gtttgtgaca ggattgtacg attccattcc ggatcttgtt tcgatcgtag agttgtcgtg
     2101 tcttgtcttt cttgatgaca ctcaacggtt ctgtctctat cttctcataa gttgtaaaac
     2161 gttttccgca ttcgtcacat tgtcttcttc tacggatcgc attgttgtct tctgccggtc
     2221 tggaatctac gactctggaa ttctcacttc cacaatatgg gcatctcatc ttcttgtacc
     2281 tccgtgtttt tactgcttga agtatatcat tttttctgta gaaaagaaag cttttctttt
     2341 atttcaggca ttctttttcg ttgatatcga ctagaatcgt atctctgccg atccttacaa
     2401 tgcatttgta ggggattttg taaactttat tcttgccaac gcatatgagc atcttcgttg
     2461 ttttgggaac gatcagataa cagatcttac ccgtatggat ttcaaattcg aggtctagga
     2521 caaagcctaa acattctcct gtattacaat tgatcacatc tttttgccga agttcgagaa
     2581 atttcatgtc aggaatcctt tttctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccatg
     2641 aatagaagca gaaaaaagca ggagattctg atctctcgaa tctcctgctt ctttattatt
     2701 ataatgatat aaagaaagat taagcttctt tctttggtgc tggtttctta ggaactaatt
     2761 tctttcctgt aatgattcct tttggacatt tacctgcaca taatccacaa cctacacatt
     2821 tatcataatc gatctgtgcg atgttgtttt caactttgat cgctccaagt ttacatactt
     2881 tttcgcatgc tttacatccg atacatcctg ctttacatac agacataacg tttttaccga
     2941 attctttaga gctgcactgt actcttgttt tcttgttttc tggtacaagg tcgatcaatg
     3001 cttttggaca tgtttctgca cattttccac atcctacaca tgcttcttca tcgatgtgag
     3061 cgattccgtt cacgatagag atcgcatttt ctggacatac tgctgcacag cttccaaatc
     3121 ccatacatcc aaattcgcat ccttttggtc ctgcacctgg tacgttcatc gcttcgatac
     3181 agctctgaac tcctgagtac tggtatttgt cagatgcttt ttcgcaagtt ccggcacatt
     3241 ttacgaatgc gactttcttc acgaattctc ctgcttctac acccatgatc gcactgatct
     3301 tctcagctgc agctgctcca ccaactggac agccgttcac tggtgcttct ccatttgcga
     3361 ttgcttttgc aagtccatca caacctgcgt atccacaacc accacagtta tttcctggaa
     3421 gttctgcacg gatggctact tccttctcgt ctacaggaac tttaaatttc tctgaggcaa
     3481 ttcccagtaa gattgcgata agcagaccgg ttcctccgac aacaaccatg gcaagtaaaa
     3541 ttgctgatat attcatcttc tttacctcct aaataagtcc tgagaatccg aagaatgcga
     3601 ttgccattaa tcctgctgtt aacagtgtga taggcattcc tttgaaagat tctgggatgt
     3661 tgttgtattc catcttttca cggataccag caaggataac aatagcgatc aggaatccta
     3721 aagagatac
//
LOCUS       DAOMUE010000200         3728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6977_length_3728_cov_2.693983, whole genome shotgun sequence.
ACCESSION   DAOMUE010000200 DAOMUE010000000
VERSION     DAOMUE010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6977_length_3728_cov_2.693983"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLC71_08445"
     CDS             complement(<1..626)
                     /locus_tag="PLC71_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM02708.1"
                     /translation="MKKRKIFTVVLIFSILMATLAGCDKADKVSDTTKKPVIKIGSDN
                     YPPYNFLNEDGIPTGIDVELATEAFRRMGYKTEIVQINWEKKKELVESGKIDCIMGCF
                     SMEGRLNDYRWAGPYIASRQVVAVNENSDIHKLSDLKGKNLAVQSTTKPEGIFLKRTD
                     KRIPKLGNLISLGHRELIYTFLAKGYVDAVAVHEESIIQYMKDYDASFR"
     regulatory      complement(699..774)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(824..2227)
                     /locus_tag="PLC71_08450"
     CDS             complement(824..2227)
                     /locus_tag="PLC71_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HRM02709.1"
                     /translation="MLGIIIDSISTFMYSKLLVILLIGAGIYFTIRTKLPQMRLFKDA
                     CKAVVEKPEDENGVSSFQALMVSTASRVGTGNIIGVSSAICIGGFGSVFWMWIIAIIG
                     SASALIESTLAQIYKRKGKDGECYGGPAYYIEAALHCRPLAIIFCISMIATYAFGFNM
                     LASYNLQSTFSGFSFYDVKWTPWIIGGILAVITGWCLLGGGSRIVKVTSTLVPAMGVA
                     YILISVIVMIINRAYLPVVFSKIISEAFDFKAIFGAFAGSAMMQGIRRGLYSNEAGIG
                     SAPNASASAQVSHPIKQGLVQMLSVFIDTLLLCTATAMMCLSSGIAPAQELQGAPWVQ
                     AALHESLGNFGPLFITVAMVLFAFTTLLGNCFYCDNLLNYIHKGQPSKTFMKGFRIVS
                     ALVVFIGAGMEVSMLWNISDVLMGVMALINIPVILILSNTALKALEDYEKQKKNGENP
                     VFKTGNIGLEYETDYWS"
     gene            complement(2532..>3728)
                     /locus_tag="PLC71_08455"
     CDS             complement(2532..>3728)
                     /locus_tag="PLC71_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM02710.1"
                     /translation="CAALTNVFICVALGAGVGAGVLVSRYFGARDYGKMKTIVSTSLI
                     SFLVLSIFLGVFGFCFSHLMMSVLQTPADILNEAVLYLRVYFVGFPFLFMYNILSTMF
                     TSIGESKIPLALLIFSSMLNILMDLWMVAGLGLGVFGAALATLIAQGISAVLSLLIFL
                     CRMRRYESRFDWFDRQELHSMLQIAVPSVLQQSTVSIGMMIVQAVVNPFGTQALAGYS
                     ATMRVENVFSLIFVSIGNAVSPYVSQNLGAKKIERIKKGYHAALVLDICFAILAFMII
                     ESLHTQISSLFLGKDGTALAYQVSGNYMRWLGYFFTFMGIKMATDGVLRGLGIMRPFL
                     VANMVNLAIRLSVALIFAPRFGIAFVWLAVPAGWFANFLISYGALRKSWPSDNCNCVI
                     NTNELL"
BASE COUNT         1151 a          858 c          623 g         1096 t
ORIGIN      
        1 cggaagcttg catcataatc tttcatatac tggatgatgg attcctcatg cacagccaca
       61 gcatccacat acccttttgc cagaaatgta tagatcaatt ccctgtgtcc aagactaatc
      121 agattgccaa gttttggaat tctcttatct gtccgcttca gaaagatacc ttccggtttg
      181 gttgtagact ggactgcaag attctttcct ttcagatcac tcagtttatg aatgtcactg
      241 ttctcattta cagcaaccac ctgacggctt gctatgtatg gtccggccca acgataatcg
      301 ttaagtcgtc cttccattga aaaacatccc atgatacaat caatttttcc actttctacc
      361 agctcttttt tcttttccca attgatctga acaatctccg tcttataccc catccttcta
      421 aatgcttccg ttgccagttc aacatctata cctgtcggga taccatcctc attcaggaaa
      481 ttataaggcg gataattgtc acttccaatt tttataacag gtttttttgt tgtgtcagac
      541 actttatctg ccttatcaca tcctgccaaa gttgccatta atatagaaaa tatcaataca
      601 acagtaaaaa tttttctttt tttcatctta taacctcatt tttatatctc atccttatga
      661 aataaaacag cactgccaaa cagacagtgc ctatcatttt ctgcaactgg ctgccatttt
      721 acaggccctt tagctttgcg tcacagactt tcgtctgttt tgcctagaat ctttctttaa
      781 ttataccatg ataatgtcag tatcaaaagt aaaaaatata aatctagctc caataatccg
      841 tctcgtattc caatccaata ttcccagtct taaacactgg attctctcca tttttcttct
      901 gcttttcata atcctccaat gctttcagtg ctgtatttga caaaatcaag atcaccggaa
      961 tattgatcag tgccataact cccatcaaaa catctgaaat attccaaagc atagaaactt
     1021 ccattcctgc accaatgaac acaaccagtg cagatactat acgaaatcct ttcatgaatg
     1081 tctttgatgg ctgtcctttg tggatataat ttaacaagtt atcacaataa aaacaattcc
     1141 caagaagtgt tgtaaatgca aacaatacca tcgctactgt gataaagagt ggtccgaagt
     1201 ttcctaagga ttcatgcagt gctgcctgta cccatggtgc cccctgtaat tcttgagcag
     1261 gggcaattcc tgatgacaaa cacatcatgg ctgttgctgt gcataaaagc agggtatcaa
     1321 tgaatacgga taacatctgt actaagccct gtttgattgg atgacttact tgtgcggatg
     1381 cagaagcatt tggtgctgat ccaatacctg cttcattgga gtacagacct cttctgattc
     1441 cctgcatcat agctgatcct gcaaatgcac caaaaattgc tttgaaatca aatgcttcgc
     1501 tgatgatctt tgaaaataca accggcagat atgcacggtt gatgatcata acgatcacag
     1561 agatcagaat atatgcaacc cccatagctg gcactaatgt agatgttact tttacaattc
     1621 ttgatccccc gcctaagaga caccatcctg tgatcactgc aagaattcct ccgatgatcc
     1681 atggcgtcca ttttacatca taaaatgaga atcctgaaaa tgtagattgt aaattataag
     1741 atgccagcat attaaatcca aatgcatacg ttgcgatcat ggaaatacag aatatgatcg
     1801 ctaatggtct gcagtgaagt gctgcttcaa tgtaatatgc cggtcctccg taacattctc
     1861 catcttttcc ttttctctta taaatctgtg ctagcgtact ttcgatcagt gcggatgcac
     1921 ttccgatgat cgcaatgatc cacatccaga atacagagcc aaatcctcca atgcagatag
     1981 cggacgatac tccaatgata tttcctgttc caactcgtga tgcggtagat accattaatg
     2041 cctgaaacga tgatactcca ttttcatctt ctggcttttc tacaacggct ttgcaggcat
     2101 ctttaaataa tctcatctgt ggcagttttg tacggatcgt aaaatagatt cccgctccga
     2161 tcaacagtat tactagcaat ttactgtaca taaaagtact gatgctgtca atgatgattc
     2221 ctaacataaa ttctctcctt gtacgatatt ctcgtataat aatctggatc aatagttaca
     2281 atttttattg cagctacttt aattcaacag aaaaatagac agtccctgcc taagggactg
     2341 cccattcatt ttaaaagtat gcgattttac tgtctgcgtc gtttttgtat cttatgatat
     2401 aatcgcgatt ttcagaattc tgataaatga tgaatatccc gttttcctgc ctgttgtggc
     2461 agaaaggtcc gttgaacctt cttcgtatgg cttcagaagc tttcgctgct ggtttaaaaa
     2521 cctatctgtt attataataa ctcattcgta ttgattacgc aattacaatt atcacttggc
     2581 catgattttc tgagtgcccc ataggagatc agaaagttgg caaaccagcc agctggtaca
     2641 gcgagccaga caaatgcaat accaaaacgc ggtgcaaaaa tcaaagcaac tgacaagcgg
     2701 atcgcaagat ttaccatatt agcaacaaga aatggccgca tgattccaag accgcgaagg
     2761 actccatctg ttgccatctt aattcccatg aaggtgaaga aataaccgag ccatctcata
     2821 taatttccag acacctgata tgctaaagca gttccatctt tccctaggaa cagtgaagaa
     2881 atctgggtat gcaaggattc gatgatcatg aaagcaagga ttgcgaaaca tatatctaac
     2941 accaatgcag catggtatcc ctttttgata cgttcgatct tctttgcacc aagattctga
     3001 gaaacatacg gcgaaactgc attaccaatg gatacaaata tcaatgaaaa aacattttct
     3061 actctcatcg ttgctgaata tcctgcaagt gcctgtgtac cgaatggatt cacaactgcc
     3121 tgtacgatca tcatgccaat tgacactgtg gactgctgaa gaactgacgg tacagcaatt
     3181 tgaagcatag aatgtaactc ctgtctgtca aaccagtcaa agcgactttc atatcgacgc
     3241 atccggcaca agaagatcaa aagtgacaac actgcggaaa tcccctgtgc aatgagagtt
     3301 gcgagggctg caccgaacac accgagtcca agtccagcta ccatccaaag atccatcaat
     3361 atatttaaca tcgatgagaa tattaaaaga gccaacggta ttttcgattc accgatcgaa
     3421 gtaaacattg ttgaaagaat attatacata aacagaaacg gaaatcccac aaaataaact
     3481 cttagataca ataccgcttc attcagtata tcagcaggtg tttgtaatac actcatcatc
     3541 aaatgagaaa aacaaaaacc aaagacaccc aaaaatatac ttaagaccag aaaactgatc
     3601 aatgatgttg acacgatcgt tttcattttg ccataatctc tagctccaaa atagcggctg
     3661 acaagcacac ccgcacctac acccgcaccc aatgctacac aaataaatac atttgtgagg
     3721 gcagcaca
//
LOCUS       DAOMUE010000201         3722 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_6994_length_3722_cov_2.423507, whole genome shotgun sequence.
ACCESSION   DAOMUE010000201 DAOMUE010000000
VERSION     DAOMUE010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6994_length_3722_cov_2.423507"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLC71_08460"
     CDS             complement(<1..216)
                     /locus_tag="PLC71_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HRM02711.1"
                     /translation="MQEFLQPLLPEEERHYLILSRQGDMNARNLLVEHNLRLVAHIVK
                     KYHNLDREKEDMISIGVIGLIKAINTYD"
     gene            complement(314..1595)
                     /locus_tag="PLC71_08465"
                     /pseudo
     CDS             complement(314..1595)
                     /locus_tag="PLC71_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(1582..2244)
                     /locus_tag="PLC71_08470"
     CDS             complement(1582..2244)
                     /locus_tag="PLC71_08470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HRM02712.1"
                     /translation="MIVDLNITEYIRSLMKEEKNDLSHIESEARVNHVPIVKPETKEL
                     LRTLVLLKKPMKILEVGAAVGFSSLYMNSYQPEGGTIITIERNEKRIKKAKENIKNQG
                     KEEQITLLEGDAIEILKGLDGSFDLIFVDAAKGQYIHFLDDVLRLLAPEGVLVSDNVL
                     QDGDVARSRYAIERRDRTIHKRMRDYLYTIKNHPQLETTILPVGDGVAISIKMGESNE
                     RR"
     gene            complement(2241..2642)
                     /locus_tag="PLC71_08475"
     CDS             complement(2241..2642)
                     /locus_tag="PLC71_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02713.1"
                     /translation="MSLSEEKKFFHSEGWQLFFRTLSRVFLICAIFCIFYGAYFVAEQ
                     TFSNQPYNAQSHKKVTVIVTQNESDEKVAETLVSKKLIYGKTRFVIRKYFSKYKDKSF
                     ISGTYEFTQSQGMDDIMGILCGDHVSEEIIQ"
     gene            complement(2644..>3722)
                     /locus_tag="PLC71_08480"
     CDS             complement(2644..>3722)
                     /locus_tag="PLC71_08480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRM02714.1"
                     /translation="STNVERQGYTPSEKNVGKRLDNLFAEHLKGRMIIATFASNVDRV
                     QHIINSAHKYGRKVAVEGRSMVNIIATASELGYIKIPKNTLIETDQIKNYPDDQIVLI
                     TTGSQGETMAALSRMANSTHRKVSIVPSDTIIFSSSPIPGNEKNISKIMNELALKGAQ
                     IVFEDTHVSGHACREELRLIYALTKPKYAIPVHGEYRHLMRHRELAIEMGIPKENAFV
                     LAEGDVLELNKRQGKIAGKVQAGGILVDGLGVGDVGNIVLNDRKLLSQNGLIIATVTL
                     QKHGNMILAGPDIVSRGFVYVRESESLMEGARDVARDAVEECLERNITDWARIKTKIK
                     DELSNYIWKKTKRNPMILPIITEV"
BASE COUNT          926 a          858 c          625 g         1313 t
ORIGIN      
        1 atcatacgta ttgatcgctt tgatcagccc gatcacaccg atcgagatca tatcttcttt
       61 ttccctgtcc agattatgat atttctttac gatatgtgca accaaacgaa ggttgtgttc
      121 cactaacaga tttctcgcat tcatgtctcc ctggcggctt aggatcagat aatgacgttc
      181 ttcctctggt aataaaggtt gtaaaaactc ttgcaaaaga aaagcaccct tctaagcctt
      241 tttatcatac tatgatactt tgcttagaaa agtgcttttc catttctatt ctgaagtttt
      301 tgccgatctt atcttaattc tctccttcga cttcttctct tcttcgaagc agatatcctt
      361 ctttgatctc ttctccaaaa tcaacataga tcatctgttt tggatgtggg gcggattcca
      421 ttggtcttcc gtcttcatct tcgatcgctt tcactataac ttctttgttt gttccgtctg
      481 ggatcataac ttcgatcgtc tctccgacag agaatttatt cttctgttct aagagtgcat
      541 atcctttgtc attatgtccc tgaacgattc cgatgtatgt gcagttctta atatacgtat
      601 tgctgtcata tatctgagaa gtttcgtctg tctttccaaa ataaaatcct gttgtaaact
      661 gacggtatgt gcattttgcg atctcttctt tgtagtaatc catatttgct ctgtattttt
      721 ctacggattc cttgcagtca tcgatcgctt ttctgtaagt tcttgcaacc gttgctacat
      781 aaagagctgt cttcatacgt ccttcgatct tcaagctgtc gattcccgca tctaagatct
      841 gatcgatata ttcgatcata ttcaagtctt tggagttaaa aatatatgtt ccacgttcat
      901 tttcatacac tggcatatat tctcctggtc ttgtctgttc catcacagag tatttccatc
      961 tgcaaggatg tgtgcattct ccgctgtttg catttcttcc tgtgaagtag ttacttaaca
     1021 ggcatcttcc ggaatgagag atacacatag caccatgaat aaatgattcg atttccatat
     1081 catctgggat atgagcacga atatctttga tctcttccag agataattct cttgcagata
     1141 cgacacggct cgctccctga tcataccaga acttgtatgt tgcatagttg gtactgtttg
     1201 cctgtgtact gatgtggcgg tcaatatctg gacaaacttc ttttgcgatc ataaaaactc
     1261 caggatctga gatgatcagt gcatctggtt tgatttcttt taattcttca aaatattccc
     1321 gcacaccagc caaatctcca ttatgtgcca aaatatttgc ggattccttc taaatcttcg
     1381 ttgtgtgcca cgatattagc agtgatataa actttcactc cgtgtttatg tgcaaattca
     1441 attccttctt taacatcttc catggagaaa ttctttgctt tggcacgtag tccatacatc
     1501 tctccaccga tataaacggc atctgctcca tataacacag caacttttaa tacttccaag
     1561 ttacttgccg gaattaataa ttcaacgtct ctcattactt tctcccatct tgatactgat
     1621 tgctacgcca tcacctaccg gaaggatcgt tgtctctaac tgtgggtgat tcttgatcgt
     1681 atataaatag tctctcattc gtttatgaat ggtgcggtct cttcgctcga tcgcataacg
     1741 tgatctggca acgtctccgt cttgaagtac gttatctgaa accagcacac cttctggtgc
     1801 cagaagtctt agtacatcat caaggaaatg aatatactgt cccttggctg catctacaaa
     1861 aataaggtca aaacttccat caagtccttt taagatctcg atcgcatctc cttctagtaa
     1921 ggtgatctgt tcttcttttc cctgattttt gatgttctct tttgctttct tgattctttt
     1981 ttcatttcgc tcaatcgtga tgatcgttcc gccttccggc tggtaactgt tcatataaag
     2041 agaagaaaat cccactgctg ctccgacttc taagatcttc atcggtttct ttaaaaggac
     2101 cagagtcctt aagagttcct tcgtctctgg tttgacgatc ggaacatgat tgactctggc
     2161 ctctgattca atatggctta aatcgttctt ctcttctttc atcaatgagc ggatatattc
     2221 tgtgatattt aaatccacta tcattgtatg atctcctctg atacatgatc tccgcataaa
     2281 atacccataa tgtcatccat tccctgagac tgtgtaaatt catatgtccc tgaaataaac
     2341 gacttatctt tgtattttga gaaatatttg cggatcacaa atctagtctt tccatagatc
     2401 agtttctttg aaacaagggt ttcggcaact ttctcatctg actcattctg tgtaacaatg
     2461 accgttactt tcttatggct ttgtgcatta tatggctgat tcgaaaatgt ctgttctgcc
     2521 acaaaatacg ccccatagaa aatacagaag atcgcacaga ttaaaaatac tctgcttaat
     2581 gtcctaaaaa acagttgcca tccttcggaa tgaaaaaact tcttctcttc tgaaagactc
     2641 atcttaaacc tctgtaatga tcggcaggat cattggatta cgctttgtct tcttccaaat
     2701 gtaattacta agctcgtctt tgatcttcgt cttgatcctt gcccagtctg tgatattacg
     2761 ttctaagcat tcctctaccg catctcttgc aacatctctt gcaccttcca tcaaagattc
     2821 tgattctctg acatatacaa acccacgaga tacgatatct ggacctgcca ggatcatatt
     2881 gccatgtttc tgcagagtaa ctgttgcaat gatcaatcca ttctgagaca gtaacttacg
     2941 gtcattcaat acgatgttac caacatctcc gacaccaagt ccatcaacga ggattcctcc
     3001 tgcctgtact tttccagcga tctttccttg tctcttattg agctccagaa catcaccttc
     3061 tgccaataca aatgcattct cctttggaat tcccatctcg atggcaagtt cacgatgacg
     3121 catcagatga cggtattctc catgaacagg gattgcatac ttcggcttcg tcagtgcata
     3181 aataagtctt aattcttcac ggcatgcatg accggaaaca tgtgtatctt caaatacgat
     3241 ctgtgcacct tttaatgcca gctcattcat gatctttgaa atgttcttct catttcctgg
     3301 aattggactt gagctgaata tgatcgtatc acttggtacg atggatactt ttctgtgagt
     3361 tgagtttgcc atacgggata atgctgccat tgtctctccc tggcttcccg ttgtgattaa
     3421 aacaatctga tcgtcaggat aattcttaat ctgatcagtt tctattaatg tgttctttgg
     3481 aatcttgatg tatcctaatt cggacgctgt cgcaatgata tttaccatgc ttcgtccttc
     3541 tactgcaact tttcttccgt acttatgtgc agaattgatg atatgctgta cacgatccac
     3601 gttcgatgca aatgttgcaa tgatcatacg tccttttaaa tgttctgcga acagattgtc
     3661 cagacgtttt cctacattct tctctgatgg tgtatagcct tgtcgttcta cattcgtact
     3721 at
//
LOCUS       DAOMUE010000202         3716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7012_length_3716_cov_2.293636, whole genome shotgun sequence.
ACCESSION   DAOMUE010000202 DAOMUE010000000
VERSION     DAOMUE010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7012_length_3716_cov_2.293636"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="PLC71_08485"
     CDS             complement(<1..1395)
                     /locus_tag="PLC71_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02715.1"
                     /translation="MAKKFCTTGTCIPEKNYMVDLSNRIQQIINQYIESGQYFTINRA
                     RQYGKTTLLYLLEKELRKQDYLVLSLSFEAADEVDKSSDNQIFLSFLGLLREKYLKCQ
                     QGKDVTFHSVILAGVYDIKTLKLKLHPQEESKYNSPWNIAVDFNIEMSFSVSDIQTMI
                     QEYEQEHRTGMDVKEISRILYDYTSGYPYLVSKICQLLDERVSDVQVWTREGILSAVK
                     VLLKEPNTLFDDMTKKLLDHPQLKEMLQNILFAGVDFPFKRETPIIDLGVTFGFLKDK
                     NGIVAVSNRIFETQLYDMFLSETAVNNQMYMKVSSDRNQFIVSGMLQMPLVMQKFYEY
                     YEEIYSEKDQKFIEETGRGKIMYKISNFSDNDDVKIIDKSGPFEVIEYQRDLSVMPED
                     AQLAFFCSQMNVRKRQLKCELSRGNVTIQSGAMQWMAGDVSATTGIKGAGDFLSKTIR
                     GKVTGESVIKPEYTG"
     gene            complement(1445..2203)
                     /locus_tag="PLC71_08490"
     CDS             complement(1445..2203)
                     /locus_tag="PLC71_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRM02716.1"
                     /translation="MENETYMKRLKDRLQIEFKKQDRSGVYGYTQRSMAYNSNRIEGS
                     TLTEKQTASMFETGTLYVDDPDMIFRTKDIEEMNGHFKMFNYVMKCMEKPLSEDIIKN
                     MHKNLKEGVFEDRANGYAIGGWKKRANRVSDIQTALPQEVPEKIHQLLEKYHNAEKIT
                     LYDIAKFHAQFENIYPFQDGNGRVGRMIILKQCLDHNIVPVIIRDIHKAEYNRYLNKA
                     QHEQDYKGLEAYFEKEQKYYQESTIPMIFDFDEL"
     gene            complement(2272..2982)
                     /locus_tag="PLC71_08495"
     CDS             complement(2272..2982)
                     /locus_tag="PLC71_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM02717.1"
                     /translation="MNDQFIQGVIFDWNKIDDDSYLKQIEALKNIERLEFKNSINFFV
                     GENGSGKSTLLEAIAIAHGFNPEGGTKNYVFSTHDTHSELCDAIRVVKGYRKEKWGYF
                     LRAESFYNVATKEEEYGGIDSARYHERSHGEGFLALAQNNLQPNGLYLFDEPEAALSP
                     QRQLTLLMEIYKCARKGAQFFIVTHSPILLGIPDADIYCFDDGRIHLCEYEETESYQI
                     TEMFINNREVLLDRLLKE"
     gene            complement(3140..>3716)
                     /locus_tag="PLC71_08500"
     CDS             complement(3140..>3716)
                     /locus_tag="PLC71_08500"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRM02718.1"
                     /translation="ILASVGFDALCHCMEAYTSKIAQPFTDALSLYAMELIAGNLVKV
                     YKGEGGKEAWEKITLASTIGGMVINTAGVTLAHGMEHPASGLKDIVHGQGLAALTPVI
                     VEASHKGNHFKFAKIARIFGGVTAEDLAGKLRSLLKDIDLACTLSDLGLSEEDIPWMA
                     ENCMKVSAASIQNNPVVFTQEEIAEIYRKAM"
BASE COUNT         1026 a          780 c          507 g         1403 t
ORIGIN      
        1 tcctgtatat tctggcttga tcacagattc tccagttact tttcctcgga tcgtcttgga
       61 taagaaatct cctgctcctt tgatccctgt tgtcgcagag acatctcctg ccatccactg
      121 catggcacct gactgaatcg ttacatttcc tctggatagt tcacatttta gctgacgttt
      181 tcttacattc atctgactgc agaagaatgc taactgggca tcttctggca tgacacttaa
      241 gtctctctga tattcgataa cttcaaatgg tcctgatttg tcgatgatct ttacatcgtc
      301 gttgtcactg aaattgctga ttttatacat aatctttccc cttcctgttt cttctataaa
      361 cttttgatct ttttctgaat aaatttcttc ataatattca taaaactttt gcattaccaa
      421 tggcatttgc aacattccag atacaataaa ttgatttcga tcagatgata ccttcatata
      481 catttgatta ttaactgctg tttctgataa aaacatatca tataactgtg tttcaaaaat
      541 ccgattcgat actgctacaa ttccattttt atcctttaag aagccaaacg taactcccaa
      601 atctataatc ggtgtttctc gtttaaatgg gaagtcaact cctgcaaata aaatgttttg
      661 aagcatttct tttaactgtg gatgatctaa caactttttg gtcatatcat caaacaatgt
      721 gtttggttct tttaacaaaa ccttgactgc acttaaaatt ccttctcttg tccacacttg
      781 aacatcggat actctttcat ctaataactg acatatcttt gaaaccaaat atggatatcc
      841 tgacgtataa tcatataaaa tccgactgat ttcttttaca tccatacctg tacgatgttc
      901 ctgctcgtat tcttgtatca ttgtctgaat atcacttaca gaaaagctca tctcaatatt
      961 aaaatcaact gcgatattcc atggactgtt atatttcgat tcttcttgtg gatgtagttt
     1021 taactttaat gttttaatat cgtaaacacc tgcaagaatt acagaatgaa atgttacatc
     1081 ttttccttgc tgacatttta aatatttctc tcttagaagt ccaaggaaag ataagaaaat
     1141 ctgattgtct gaacttttgt ctacttcatc ggcagcttca aaacttaaac tcaaaaccag
     1201 ataatcttgt tttcgcaatt ctttttccag aagataaagc aaggttgtct taccatattg
     1261 tctggctcga ttaatcgtaa agtattgtcc tgattcgata tattgattga taatctgctg
     1321 aatcctgttg ctaagatcaa ccatataatt tttctccggg atacatgttc ctgttgtaca
     1381 aaacttctta gccataaacc ccaacctcct ttcttgtcca ctctctatta attacaccct
     1441 tattctacaa ttcatcaaaa tcaaatatca ttgggattgt gctttcctga taatatttct
     1501 gttctttttc aaaataagct tctaatcctt tatagtcttg ttcgtgctgt gctttattaa
     1561 gatatcgatt atattctgct ttatgaatat cacggatgat cactggaaca atattatgat
     1621 ccagacattg ttttagaatg atcatacgcc cgactctccc atttccatcc tgaaatgggt
     1681 aaatattctc aaactgtgca tgaaatttag caatatcgta taatgtaatt ttctctgcgt
     1741 tatgatactt ttctaataac tgatgaatct tttctggaac ttcttgtggc aatgccgtct
     1801 gaatatcact gactctgttt gcacgctttt tccacccacc aattgcatat ccatttgctc
     1861 gatcttcaaa aacaccttcc tttaaatttt tatgcatatt ttttatgata tcttctgaca
     1921 aaggtttttc catacatttc attacataat taaacatttt gaaatgacca ttcatttctt
     1981 ctatatcttt tgttcgaaaa atcatgtctg gatcatccac atataaagtt cctgtttcaa
     2041 acatacttgc tgtctgtttc tctgttaagg tacttccttc aattcgattg gaattatacg
     2101 ccatactcct ctgtgtataa ccgtatactc cgcttcgatc ctgtttttta aattctattt
     2161 gcagtctgtc ttttaatcgt ttcatatatg tttcattttc catgatttta tctcacctct
     2221 tttatttaat ctgttatttt taagtatagc atgaattatg aatttctaca tctattcttt
     2281 tagaagtcta tccaataata cttcccgatt atttataaac atctccgtaa tctgataact
     2341 ttccgtctcc tcatactcac ataaatgaat cctaccatca tcaaaacaat aaatgtcagc
     2401 gtctggaatt cctagtaaaa tcggtgaatg tgtaacaata aagaactgtg ctccttttct
     2461 tgcacattta tatatctcca ttaataatgt taattgtctt tgtggtgata atgccgcttc
     2521 tggctcatca aaaagataca atccattggg ctgtagatta ttttgtgcta atgccaagaa
     2581 accttctccg tgtgatctct catgatatct ggctgaatct attccaccat attcttcttc
     2641 cttcgttgca acattataga aactttctgc tctaagaaaa tatccccatt tttccttccg
     2701 atatcctttt acaactctga tggcatcgca taattcggag tgtgtatcat gcgtagaaaa
     2761 tacatagttt ttcgttccac cttctggatt aaatccatga gctatggcaa ttgcttctaa
     2821 taaggtggac tttccacttc cattttctcc aacgaagaaa ttgattgaat ttttaaattc
     2881 tagcctttca atatttttta atgcttctat ttgttttaga taactgtcat catctatttt
     2941 attccagtca aatattacgc cttgtataaa ctgatcattc attggatttt cctttctatc
     3001 atgataagct tcttcctaat ccttttatat tttatcaaaa aaagatatga cacgaaactg
     3061 tcttttcata tcatatcttt catatttctt atattttcat ttttctacat actcaatagc
     3121 ctatagataa taaactatcc tacattgctt tcctgtatat ctcagcaatt tcttcctgtg
     3181 taaatacaac cggattgttc tgaatacttg ccgcagatac tttcatacag ttctctgcca
     3241 tccaaggaat atcttcttcg gataatccaa ggtcagatag tgtacaagca aggtcgatat
     3301 cttttaacag acttcttaat ttaccagcaa gatcttctgc ggtaaccccg ccaaagattc
     3361 ttgcgatctt agcaaatttg aaatggtttc ctttatggga tgcttctacg attactggtg
     3421 ttaatgctgc aagtccttgt ccgtggacga tatcttttag tccgctggct ggatgttcca
     3481 ttccatgtgc cagagtgaca cctgctgtat taataaccat tccaccgatc gttgatgcca
     3541 gagtgatctt ctcccatgct tctttgccgc cttcaccttt atatacttta acaaggtttc
     3601 ctgcgatcag ttccatcgca tataaactta gggcatctgt aaatggctgt gcgatctttg
     3661 atgtgtatgc ttccatgcag tggcataatg cgtcaaatcc aacagatgca agtata
//
LOCUS       DAOMUE010000203         3712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7019_length_3712_cov_2.605414, whole genome shotgun sequence.
ACCESSION   DAOMUE010000203 DAOMUE010000000
VERSION     DAOMUE010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7019_length_3712_cov_2.605414"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..173
                     /locus_tag="PLC71_08505"
     CDS             6..173
                     /locus_tag="PLC71_08505"
                     /inference="COORDINATES: protein motif:HMM:NF016925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HRM02719.1"
                     /translation="MDDPDVREPLKFLREREIVHFQRFGEALRTVQDHLDSKNFYAIN
                     PAFDHSGNCNK"
     gene            328..762
                     /locus_tag="PLC71_08510"
     CDS             328..762
                     /locus_tag="PLC71_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM02720.1"
                     /translation="MKINIEFPRKLLEVYNDACKPLCKKLKLPQTAFDILMFLGNNPQ
                     YQTARDIVKIRNIKANLISINVEKLVKEGYLRREEIKGDRRKTKLIITEKAHPVIKEG
                     QQLQQGFVDQLFDNTTQEDKKIFIHVMKNMDKNLDDILKGGN"
     gene            764..1540
                     /locus_tag="PLC71_08515"
     CDS             764..1540
                     /locus_tag="PLC71_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRM02721.1"
                     /translation="MNILLTIAVTFFAGMGAGLGTGFAGMSAAAVISPMLITFLKMDP
                     YMAVGIALSSDVLASAVSAYIYGKNKNLDIKNGIIMMISVLTFTVVGSYIASLLPAAT
                     MGSFSVFMTFLLGIKFIVRPVMITKEAMQGVSAKKRAIQSVVCGIMIGLICGFVGAGG
                     GMMMLLILTSVLGYELKTAVGTSVFIMAFTAFTGAVSHFMIVGAPDWTVFILCVVFTL
                     IWARIAARFANKATPETLNRATGVILVILGIVVLGFSMLS"
     gene            1569..>1972
                     /locus_tag="PLC71_08520"
     CDS             1569..>1972
                     /locus_tag="PLC71_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HRM02722.1"
                     /translation="MKKNLVVIEELKKQGKALLDARRDNFVRQSLLSDEFLNFMQQNK
                     KPFDLLMSYYECAIMEVETKFRVLNHELSLEYDNNPIESIKTRVKSYDSILKKIRRKN
                     IPLNLRAIEDNLKDIAGVRVICSFPDDIYKLA"
     assembly_gap    1973..1982
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1983..2353
                     /locus_tag="PLC71_08525"
     CDS             <1983..2353
                     /locus_tag="PLC71_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HRM02723.1"
                     /translation="KQDDITLIERKDYIKNPKPSGYRSLHLIVQVPIFLQNEKKMVNV
                     EVQFRTIAMDFWASLDHKMRYKKELSDEEVEILQEELYDCARQSAALDERMQGIRDRI
                     TKKQEQETILLEDKNSKDWL"
     gene            2528..>3712
                     /locus_tag="PLC71_08530"
     CDS             2528..>3712
                     /locus_tag="PLC71_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM02724.1"
                     /translation="MIDLIRRQIELLKLLSKQREYKPASFFSKSLDVSTKTIYTDITY
                     LQSEVEKYKVDLVRAPRIGIRLEGEKENIQHMLRDLQKDNLSEDEKYTPEYRRLWILK
                     KVLIDCETITLESVSKEFLVSKTSLYQDIAVINKSIESQSDVKLEVGECGICILGEEI
                     EIQNAVNNYLLSESKEEMFSDFTHKLGNFFELDVIKAVSDLILNDFEELTEVLSEYYL
                     KSLLVTLIMQSSRLLKKKHMNEETEISYNNIRHMETYIVANSIAEQLKYQLHITYSNN
                     DMEYLCRQLYAHRVTHHVKHMRTDYEETVRDVIKRMSEIQKLDLTRDKKLYESLLYHL
                     PPMILRLQNNIQIVNPMLDNIKQEYPELFTTMWYALTQIELRYQVTLTEDEVSLILLH
                     FQV"
BASE COUNT         1383 a          476 c          756 g         1087 t
ORIGIN      
        1 ggcttattga tgatccagat gttagagagc cactgaaatt cttacgagaa agagaaatcg
       61 tgcatttcca gagatttggt gaagcactga ggactgtaca ggatcatttg gattctaaga
      121 atttttatgc gatcaatcca gcatttgatc atagtggtaa ttgtaataaa taaatatata
      181 aaaacagttg gcataaaaag taaaaattat gtcagctgtt ttttattttt tgaagtaaca
      241 aaaagttgaa aatatatgac aatctgttat aataaatcaa aaatgaatag ttcaaatatg
      301 aattaattaa taaggaggga taaattcatg aagatcaata tagaatttcc gagaaagttg
      361 ttagaggtat ataacgatgc atgtaagccg ttgtgcaaga aattaaaatt accacagaca
      421 gcttttgata ttttaatgtt tttaggaaac aatccacaat accagacagc cagagatatc
      481 gtaaagatca gaaatatcaa agcaaatctt atatcaatca acgtagagaa acttgtgaaa
      541 gaaggttatt taagaagaga agagattaaa ggtgatcgaa gaaaaacaaa actgattatt
      601 acagaaaaag cacatccagt gatcaaagaa ggacagcagt tacaacaagg atttgtagat
      661 cagttgtttg ataatacaac acaagaagat aaaaaaattt ttatccatgt aatgaaaaat
      721 atggataaga atttagatga tattttgaaa ggtggaaact aagatgaata tattattaac
      781 aatcgcagta acattttttg caggaatggg agccgggctt ggaacaggat ttgcagggat
      841 gagtgcagca gctgtaatta gtccgatgtt gatcacattc cttaagatgg acccgtatat
      901 ggcagttgga attgcattgt cctctgatgt attagcaagt gcagtatcag cttatattta
      961 tggaaaaaat aagaatcttg atataaaaaa tggtatcatc atgatgataa gtgtgttaac
     1021 atttacagta gtaggaagct atattgcaag cttacttcca gctgcaacga tgggaagctt
     1081 ttctgtattt atgacatttt tattaggaat taaatttatc gtgagaccag ttatgataac
     1141 caaagaagcc atgcaaggag tttcagcaaa gaaacgtgca attcagtcag tggtctgtgg
     1201 gatcatgatc ggattgatct gtggatttgt tggtgcaggt ggtggaatga tgatgttgct
     1261 cattttaacg agtgtattag gatatgaatt aaaaacagct gttggaacga gtgtatttat
     1321 tatggcattt actgcattta caggagctgt ttctcatttt atgattgttg gagcaccgga
     1381 ttggacagta tttatcttat gtgttgtatt tacgctgatc tgggcaagga tcgcagcacg
     1441 atttgccaat aaagcaacac cagagacatt aaatcgtgcg acaggtgtga tccttgtgat
     1501 cttagggatt gtagtattag gattttctat gctttcataa agaatattta cgaaggaagg
     1561 aatttttaat gaagaaaaat ctggttgtga tcgaggaatt aaaaaaacag ggaaaagcat
     1621 tattagatgc gagaagagat aactttgtca gacaaagtct tttgtcagat gaatttttga
     1681 attttatgca acaaaataag aagccgtttg atcttttaat gtcatattat gaatgcgcga
     1741 tcatggaagt ggaaacaaaa ttcagagtat taaatcatga attatctttg gaatatgata
     1801 ataatccgat tgagagtatt aagacaagag tcaaatcata cgatagcatt ttgaagaaga
     1861 tccgaagaaa aaatattcca ttaaatctaa gggcgattga agataattta aaagatattg
     1921 ccggggtcag agttatctgt tcatttccag atgacattta taaattagca gannnnnnnn
     1981 nntaaagcaa gatgatatca cactgatcga gcgaaaagat tatattaaga atccaaagcc
     2041 aagtggatat cgaagtcttc atctgatcgt acaagtaccg atctttttac aaaatgaaaa
     2101 gaagatggtg aatgttgaag tgcaatttcg aacgatcgct atggatttct gggcaagtct
     2161 tgaccataag atgcgttata agaaagagtt gtctgatgaa gaagtggaaa tccttcagga
     2221 agaactttat gattgtgcaa gacaaagtgc agcacttgat gagagaatgc agggaatcag
     2281 ggatcgtatt acaaagaaac aagaacagga aacgatattg ttagaagata agaacagcaa
     2341 agactggtta taaaacataa gtgtgacaaa cgtacatata ggagatatca tatatcagga
     2401 aatgatcata gatatctcct ttttttattc gtttccacat taatatggaa gctaatgtaa
     2461 ttgcaagaaa taagatatca atggtattct agttacagaa agaaaaacgt taattaacag
     2521 attaggggtg attgatttga ttcgtcgaca aatcgaatta ttaaaacttt tgtcaaaaca
     2581 aagggaatat aaacccgcat ctttctttag taaatcatta gatgtttcga caaaaacgat
     2641 ttacacagat attacttatc ttcaaagtga agttgaaaag tataaagttg atctggtcag
     2701 agctccaaga ataggaattc gtctggaagg tgaaaaggaa aacattcagc atatgttaag
     2761 agaccttcaa aaggataatc tgtctgaaga tgaaaaatat acaccggagt atcgcagatt
     2821 gtggatttta aagaaggtat tgatcgattg tgaaacaatc acattggaat cagtatcaaa
     2881 ggaattttta gtaagtaaaa catcactgta tcaggacatt gcagtgatca ataaatcaat
     2941 cgaaagtcag tcagatgtaa aactggaagt tggagaatgt ggaatctgta ttttaggaga
     3001 agaaatcgag atacaaaatg cagtgaacaa ttatttgtta tcagaatcaa aggaagaaat
     3061 gttttctgat tttacgcaca aacttggaaa cttttttgaa cttgatgtga taaaagcagt
     3121 ttcagatctt attttaaatg attttgaaga actgaccgaa gtgctttcag aatattatct
     3181 gaaatcttta ttggtaacat tgatcatgca aagttcaaga ttgctcaaaa agaaacatat
     3241 gaatgaggaa acagaaattt cttacaacaa tatccggcat atggagacat acatcgtggc
     3301 aaatagtatt gcggaacagc tgaaatatca gcttcatatt acatacagta ataatgatat
     3361 ggaatattta tgcagacagt tatatgccca cagagtgaca catcatgtaa aacatatgcg
     3421 gacagactat gaagaaacgg taagagatgt catcaagaga atgagtgaga ttcaaaagct
     3481 tgatctgaca agggacaaga aattatatga gtcattgtta tatcatctgc caccgatgat
     3541 cttaagattg caaaataata ttcagatcgt aaatccgatg cttgataata tcaaacagga
     3601 gtatccggaa ctttttacaa cgatgtggta tgcacttaca caaatagaat taaggtatca
     3661 ggtaacatta acagaagatg aagtttcact aatcttatta cattttcaag tg
//
LOCUS       DAOMUE010000204         3682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7087_length_3682_cov_2.511166, whole genome shotgun sequence.
ACCESSION   DAOMUE010000204 DAOMUE010000000
VERSION     DAOMUE010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7087_length_3682_cov_2.511166"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1665)
                     /locus_tag="PLC71_08535"
     CDS             complement(<1..1665)
                     /locus_tag="PLC71_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HRM02725.1"
                     /translation="MQKVRKLVTTIMIAALITAMEGTTILPLSHHVKADTNTVSQTSQ
                     AENDLTKYKKINGIGDNTVLGADFSHYQLQKNAWKKVWKNYKGIEVSNVFEYVRSQGI
                     NTISVKVAVNPAKDKDGNESYLSLENAKKTLKEAKKAGLKTNVTLLYSDDITYAGVQK
                     LPDGWDTDSAEEKALEYTKNVIKELKAADAVPTMITIGNEVNYNFLNMSSGDGWEGFV
                     AMSKISKMIREEGIKPAVSVSAPTADASDIQWIIGKLGDADVDYDYIGVNIYPNTHND
                     NYVKTLKNTVEEKAAGKQMIISSVKCPWKDSEGKASITTQTKSIYDYLQATIDEKNAG
                     GLIYDDADFVGAWDSFFDENGQAMSSLAIFAYAQGNQVDVSSYKDPWEYGGDTGLKDQ
                     KVTIKKVKGMSESSIRGMDISSYLALKKAGVKYYDYEGNETPLLKVLHDNGINYIRIR
                     IWNDPFNADGETYGGGGNDVSTGVEIAKEAAQYDMKVLLDFHYSDFWAEPAVQLIPKA
                     WKKDVNNTEKMCSDVYDFTKESIQKFKDGGANIGMVQVGNEITNGLL"
     gene            complement(1680..>3682)
                     /locus_tag="PLC71_08540"
     CDS             complement(1680..>3682)
                     /locus_tag="PLC71_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HRM02726.1"
                     /translation="YAQGNQVDVSSYKDPWEYGGDTGLKNLTASVKKLNNMSQSSVRG
                     MDISSYTALKKAGVKYYDFDGKETSLLKVLHDNGVNYIRIRIWNDPTNEKGETYGGGA
                     NDVAAGLEIAKEAAQYDMKLLLDFHYSDFWADPALQKIPKAWEKDKNDTEKMKQNVYD
                     FTKDTIKKFQEVGADIGMVQVGNEITNGMLDIMPDYSKGETYKDTWGNAKNAKILCGY
                     LKAGIKAVRECTPKALVTLHLESMGYGKCSEIMNAWERNGVDYDVFGSSFYQFWQGNS
                     SKNALAGLQKIENLAKSRGKMYAVMETSWLNSLKDADGTSNVIGEGHANAKVYSDDPQ
                     GQVDALTDMYQTLLSNDNGLGAFYWEGAWIPVKAGWTNWKYNKDMSDRYGTGWAAQGA
                     KGYYPDNKMYYNGQPAWGGSSWDNQTLFDSNGYPLQSLKFYKDSVSKGKEQIIALKIV
                     DKNGKEVYPTQYVKVEVGKTRKITLPKFSGYYPKNKKYNMTLKGTQEGNTVQKVVYTR
                     TAAGPAISYNYRVKVTKKNYKLYKNFKWKKSKTKVYKKTYVAKYRYDHKNGNKYLALY
                     TKGGKFVGYINKKAVKRLGSATQPEQGKAYTYGKRVKIKSKKYKLYKNFKWKKSKTKV
                     YKKTYVAKYRYKHENGNKYLALYTKSGKFVGYINTKAAKVVK"
BASE COUNT          907 a          815 c          513 g         1447 t
ORIGIN      
        1 aagaagtccg ttggtaattt catttcctac ctgaaccatt ccaatgtttg ccccgccatc
       61 tttaaatttc tgaatagatt ctttggtgaa atcatataca tctgaacaca tcttttctgt
      121 attatttaca tcctttttcc atgcttttgg aattaactga accgctggtt ctgcccagaa
      181 atccgaataa tgaaaatcaa gcagaacttt catatcatat tgggctgcct cttttgcgat
      241 ctctacacct gtggaaacat catttccacc accgccatag gtttccccat ctgcattaaa
      301 cggatcattc cagatcctga tcctgatata attgattcca ttatcatgaa gtacttttaa
      361 taacggtgtc tcatttcctt cataatcata atatttcact cctgctttct ttaatgcaag
      421 ataagatgaa atatccattc ctcggataga actctcagac attcctttga cttttttgat
      481 cgttactttt tgatctttta atcctgtatc tccaccgtat tcccatggat ctttgtagct
      541 gcttacatca acctgatttc cttgtgcata agcaaagatt gcaagtgaag acattgcttg
      601 tccattttca tcaaagaatg agtcccatgc cccgacaaaa tctgcatcat cgtagatcaa
      661 tccacctgca tttttctcat cgatcgttgc ctgaagataa tcataaatgc tctttgtctg
      721 tgtcgttata ctggcttttc cttctgaatc tttccatgga catttgacac tggaaatgat
      781 catctgcttt cctgctgcct tttcttctac tgtattcttt aatgttttta cataattgtc
      841 attgtgtgta tttggataga tgtttactcc aatataatca taatcaacat cagcatctcc
      901 taattttcca atgatccatt gaatatctga agcatctgct gttggtgccg atacactaac
      961 agccggtttg attccttctt ctctgatcat tttcgagatt ttgctcattg caacaaagcc
     1021 ttcccatcca tctccactac tcatatttaa gaaattatag tttacttcat ttccgatcgt
     1081 gatcatcgtt gggactgcat ctgctgcctt caattctttg atgacgtttt ttgtatactc
     1141 cagtgctttt tcttctgctg aatcagtatc ccaaccatct ggtaatttct gcacccctgc
     1201 atacgttata tcatcagaat ataacaatgt aacatttgtt tttaatcctg ctttcttggc
     1261 ttcttttaat gtcttcttag cgttttctaa agacagatag ctttcattac catctttatc
     1321 ttttgctggg ttcactgcaa ctttcacaga aatcgtattg atcccctgtg atcttacata
     1381 ttcaaacaca ttggaaactt cgattccttt gtaatttttc cagacttttt tccacgcatt
     1441 tttttgcagt tgataatggg aaaaatctgc tcctaaaacc gtgttatctc caattccatt
     1501 gatttttttg tatttcgtca gatcattctc tgcctgactg gtttgtgaga cagtattcgt
     1561 atctgctttt acatgatgtg ataacggtaa aattgttgtt ccttccatcg ccgtgatcaa
     1621 tgctgcgatc attatcgttg ttaccagttt tcttaccttt tgcataaact accctcctgt
     1681 tatttgacaa cttttgcagc cttcgtattg atatatccaa caaattttcc tgattttgta
     1741 tacaatgcaa gatatttatt tccattctca tgtttatatc tatattttgc tacatatgtt
     1801 ttcttataaa cttttgtttt tgatttcttc catttgaaat tcttatatag tttatatttt
     1861 ttactcttga tctttacacg ttttccataa gtgtatgctt ttccctgctc tggctgtgtt
     1921 gctgatccta atcgttttac cgctttctta ttaatgtacc caacaaattt accacctttt
     1981 gtataaagag caagatattt atttccattc ttatggtcat atctatattt tgctacatat
     2041 gtctttttat aaacttttgt tttggatttc ttccatttga aattcttgta aagtttataa
     2101 tttttctttg taactttcac tcggtaatta taactgatcg caggtcctgc tgctgttctt
     2161 gtatatacaa ctttctgtac tgtattacct tcctgtgttc cctttaatgt catgttatat
     2221 tttttattct ttggataata tccagagaat tttggtaatg tgatcttgcg ggtctttcct
     2281 acttcaactt tgacatactg ggtaggatat acttcttttc catttttatc aacgatcttc
     2341 aatgctatga tctgttcttt tcctttggat acagaatctt tatagaattt taatgactgt
     2401 aatggatatc cattggaatc aaataacgtc tggttatccc agctgcttcc gccccatgct
     2461 ggttgtccat tataatacat cttgttatct ggatagtatc cttttgctcc ctgggctgcc
     2521 catccagttc cataacgatc agacatatcc ttattgtact tccaattagt ccatcccgct
     2581 tttaccggaa tccatgctcc ctcccagtaa aaagctccaa gcccattatc atttgataac
     2641 aatgtctgat acatatccgt caatgcatcc acctgtccct gtggatcatc agaatatact
     2701 tttgcatttg catgtccttc accaatgaca tttgatgtac catccgcatc ttttaaactg
     2761 ttaagccagc ttgtttccat aaccgcatac atttttcctc ttgacttggc aagattttct
     2821 attttctgaa gtccagctaa tgcattttta ctggaatttc cttgccagaa ttgataaaat
     2881 gagctaccga aaacatcata gtccactcca tttcgttccc atgcattcat aatttctgaa
     2941 cattttccat aacccatact ttcaagatgc agtgtaacta atgcttttgg tgtgcattct
     3001 ctgactgctt tgatccctgc tttcaaatat ccacataaaa tcttggcatt ctttgcattt
     3061 ccccatgtat ctttataagt ttctcccttt gaataatctg gcatgatatc aagcattcca
     3121 tttgtaatct catttccaac ttgtaccatt ccgatatctg ctccaacttc ctggaatttc
     3181 tttatcgtat cctttgtaaa atcgtaaaca ttttgtttca tcttttctgt atcattttta
     3241 tctttttccc aagctttggg tatcttttga agtgctggat ccgcccagaa atcagaatag
     3301 tggaaatcta gcagaagttt catatcatac tgtgcagctt cttttgcaat ttcaagacca
     3361 gctgccacat cattcgcacc acctccgtat gtttctcctt tttcatttgt cggatcattc
     3421 cagatacgga ttctgatata attaactcca ttatcatgta atacttttaa caatgatgtt
     3481 tcttttccgt caaaatcata gtattttacg cctgctttct ttagtgctgt ataggatgaa
     3541 atatccattc cacgaacgga actttgtgac atgttattaa gttttttgac agaagctgtt
     3601 agattcttaa gtcctgtatc tccaccatat tcccatggat ctttgtagct gcttacatca
     3661 acctgatttc cttgtgcata ag
//
LOCUS       DAOMUE010000205         3682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7088_length_3682_cov_2.437552, whole genome shotgun sequence.
ACCESSION   DAOMUE010000205 DAOMUE010000000
VERSION     DAOMUE010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7088_length_3682_cov_2.437552"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..469)
                     /locus_tag="PLC71_08545"
     CDS             complement(182..469)
                     /locus_tag="PLC71_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HRM02727.1"
                     /translation="MSKKIKTEAAKRLFQAVLTLETEEECFTFFEDLCTVNELESLAQ
                     RFEVASMLYDKHTYLEVADKTGASTATISRVNRSLNYGSDGYDMVFKRMKK"
     gene            complement(544..1971)
                     /locus_tag="PLC71_08550"
     CDS             complement(544..1971)
                     /locus_tag="PLC71_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HRM02728.1"
                     /translation="MLNTLNDVLNNVDNFIWGMPLIILILFTGILLTVRLRGLQIRHL
                     GKALHYVFHNEDDGEGEVTSFGALCTALSATIGTGNIVGCATAIVAGGPGALFWMWLA
                     AFFGMATKYAEGMLAVKYRVIAEDGHTLGGPFYYIEKGMGKNFKWLAKLFCVFGTMVG
                     LFGIGTFTQVNGITSAVNNFFDPSNVHTISLFGMNYSISVVVAGIIVTICAGLVIIGG
                     IKRISKVSEVIVPFMAVTYIGVCLIIVFTHLPQVPAAFAEIVQSAFGLKAVAGGAIGA
                     MTVAMQKGIARGIFSNEAGLGSAPIAAAAAQTNEPVRQGLVSMTGTFIDTIIVCTMTG
                     LAIVMTGAWDMGLEGVAVTTKAFALGLPFPAKASAFVLMMCLVFFAFTTILGWAYYSE
                     RCLEYLVNGKKFVITAFKWFYILAVFIGPFMTVSAVWTIADICNGLMAFPNLVALLAL
                     NGDVVSETRGYLERVGIVKTTVGEA"
     regulatory      complement(2092..2186)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2459..>3682
                     /locus_tag="PLC71_08555"
     CDS             2459..>3682
                     /locus_tag="PLC71_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRM02729.1"
                     /translation="MNKHKRSTFSGKLGFVLSAAGASVGLGNIWRFPYLAAKYGGGIF
                     LLVYIILALTFGYTLIMAETSLGRMTRKSPVGAFESFGNSRFISIGGWINAIIPILIV
                     PYYSVIGGWVIKYLIEYFAGHSKTLAGDAYFTTFISNGWSTEICFIIFTLFTLGIIYA
                     GVRNGIERVSKFMMPILIVLSLIIAVYSVTRPGAIEGVKYFLVPNFDHFSWMTVVSAM
                     GQMFYSLSIAMGILITFGSYMKKDTSIEKSTENVEIFDTAIAIMAGLMIIPAVFAFSG
                     GNAETLKSGPALMFITIPKVFANMGFGTAIGVVFFLLVLFAAVTSSIALTESAVSTFE
                     DELHWSRKKSTVFMGMVMLLLGTLSCLGYGPLANFKILGMQFLDFFDFITNSVMMPIA
                     AIATCLLVSKVVGVDK"
BASE COUNT         1056 a          759 c          641 g         1226 t
ORIGIN      
        1 ctacttttgc tgaattaaag gaatcgaaga agaaagaaca gctgcagcaa tttgctttta
       61 ttacacttct tggatatgat atctatctaa ggaaacaggt gattgagaaa atgatcgatg
      121 aactctatca accagagtca aacaataaag cagaaaagaa aaagaagaaa taggcatctg
      181 cctatttctt cattcgttta aataccatat catagccatc acttccataa ttcaatgaac
      241 gattcactcg gctgatggtt gccgtggaag cacctgtctt atctgcaact tctaagtatg
      301 tatgcttatc atataacatc gaagccacct caaatctctg agctaacgat tctaattcat
      361 ttactgtaca aagatcttca aaaaatgtaa aacattcttc ttctgtctct aatgtcagta
      421 ctgcctgaaa taatcttttt gccgcttctg tcttgatttt cttgctcatt agcatttact
      481 ccctttctga tcttatagtt ccactatact atatcagagt gttaaagtaa agcaatattt
      541 tctttatgct tctcctactg ttgttttcac aataccaact ctttcaagat atcctcttgt
      601 ctcagataca acatctccgt tcaacgcaag cagtgctaca aggttcggga atgccattaa
      661 tccattacag atatccgcaa tagtccatac agcactaact gtcataaatg gtccaataaa
      721 tactgctaaa atataaaacc atttgaacgc tgtgataacg aatttctttc catttaccag
      781 gtattccaga catctttcac tataatatgc ccatccaaga attgttgtaa atgcaaagaa
      841 taccagacac atcattaata caaatgctga tgcttttgca gggaatggaa gtcctaatgc
      901 aaatgccttt gttgtgactg caactccttc aagtcccata tcccatgcac ctgtcataac
      961 gattgcaagt cctgtcattg tacatacgat gatcgtatca ataaatgttc ctgtcataga
     1021 tacaagtccc tgacgtactg gttcatttgt ctgagctgct gccgctgcga tcggcgctga
     1081 tccaagtcct gcttcgttag agaagattcc tcttgcaata cctttctgca ttgcaactgt
     1141 catagctccg attgctccac ctgcaactgc ttttagtcca aatgctgact gtacgatctc
     1201 agcaaatgct gctggtacct gtggtaaatg tgtgaataca atgatcagac agacaccaat
     1261 atatgtcact gccataaatg gcacgataac ctctgatacc ttagagattc tcttgattcc
     1321 tccaatgata acaagtcctg cacagattgt tacgataatt cccgctacaa caacgctaat
     1381 agaataattc attccaaata agctgatcgt atgtacattg gatgggtcaa aaaagttatt
     1441 aactgctgat gtgattccat ttacctgtgt aaatgttccg attccaaaca aacctaccat
     1501 tgttccaaat acacaaaaca attttgcaag ccatttaaaa ttcttaccca ttcctttttc
     1561 aatgtaatag aatggtccgc caagtgtgtg accatcttct gcgatcactc tatacttaac
     1621 cgctaacatt ccttcagcat attttgttgc cattccaaaa aaggctgcaa gccacatcca
     1681 gaataatgct cctggtccac ctgcaacgat tgctgtcgca catcctacga tattaccagt
     1741 tccgatcgtt gctgaaagtg ccgtacataa tgcaccaaaa cttgtaactt ctccttctcc
     1801 atcatcttca ttatgaaata cataatgtag tgcctttcca agatgtctga tctgtagtcc
     1861 tcgaagcctg actgtcaata agattcctgt aaaaaggatc aagatgatga ggggcatccc
     1921 ccagataaaa ttgtcgacgt tgtttagtac atcgtttagc gtgtttaaca tttttcattc
     1981 ctccaaaaat agatctttgc gaatgtaacc ttgaacagct tgatttttgc atatctgagt
     2041 atacaaaaaa gagctgatta aatcagctct aaagagtatc gaatacattg cttataaagc
     2101 tctgtccttt tgcctgagag attagtttcc taatagaaac tgtacacctt cggcgctcac
     2161 ttatgagact ctccagagtt catttctttt tgttcatcag aaagaaatgc ctatgtttat
     2221 ttttcttatc cgcaatattt actttatatg atgattctga tatttgcaag tatttttttt
     2281 aataatcagt cactttcagc acactataca aaaacacttg tctttctatg gcatacatat
     2341 gtaatatttt ccatacaata tttttatcag tttctttgct tttttcctgc tgctgtgtta
     2401 aactgattca gatatgctca atgcgtatgg aaacattatt taaaggagat aatttataat
     2461 gaacaaacac aaaagaagca ctttctctgg aaagcttggc tttgtattat cagctgctgg
     2521 tgcttccgta gggcttggaa acatctggag atttccatat ctcgctgcta aatatggcgg
     2581 tggaatcttt ttacttgttt atatcatact tgcactgacc ttcggttaca ccttgatcat
     2641 ggcggagact tctcttggac gcatgacaag aaaaagccct gttggagcat ttgaaagctt
     2701 tggaaactca cgttttatat ctattggtgg ttggatcaat gcaatcattc cgatcctcat
     2761 cgttccttat tattctgtga tcggtggatg ggtgatcaaa tacttaattg aatattttgc
     2821 cggacatagt aaaactttag ctggcgatgc ttattttacg acatttattt ccaatgggtg
     2881 gtctacagaa atctgtttta tcatttttac attatttaca cttggtatca tttacgccgg
     2941 tgtacgaaat ggaattgagc gtgtatcaaa atttatgatg ccgatcctga tcgttttatc
     3001 actgatcatc gctgtgtatt ctgtaaccag accaggtgct attgaagggg taaaatactt
     3061 tcttgtgcca aactttgatc atttttcatg gatgaccgtt gtatctgcaa tgggacagat
     3121 gttttattct ttatccattg caatgggaat attgatcaca tttggttctt atatgaagaa
     3181 agatacttcc attgaaaaat ctacagagaa tgttgagatt tttgatactg cgatcgcgat
     3241 catggctgga cttatgatca ttcctgctgt ttttgctttc tctggcggta atgccgaaac
     3301 attaaagtct ggacctgcct taatgtttat cacaatcccg aaagtttttg caaacatggg
     3361 atttggaact gcgatcggtg ttgtattttt cttattagta ctgtttgcag ctgtgacaag
     3421 ttcgatcgca ctgacagaaa gtgctgtttc gacgttcgaa gatgaactac actggagccg
     3481 taaaaaatca actgtgttca tgggaatggt tatgttactg cttggaacgc tttcctgcct
     3541 tgggtatgga cctcttgcga attttaagat tcttggaatg caatttcttg atttcttcga
     3601 tttcataaca aactctgtga tgatgccgat tgccgcaatc gcaacctgcc ttctggtgtc
     3661 aaaagttgtt ggagttgata aa
//
LOCUS       DAOMUE010000206         3671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7113_length_3671_cov_2.988108, whole genome shotgun sequence.
ACCESSION   DAOMUE010000206 DAOMUE010000000
VERSION     DAOMUE010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7113_length_3671_cov_2.988108"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..1200)
                     /locus_tag="PLC71_08560"
     CDS             complement(334..1200)
                     /locus_tag="PLC71_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRM02730.1"
                     /translation="MQDVSKDGLHLFSSTEELTPDEVKRYDRQIILPQVGSDGQKKLK
                     KSKVLIIGAGGLGAPAALYLAGAGVGTIGIVDADDVSVSNLQRQIIHTSKREGTNKAE
                     SAKKSILELNEHIKVNTYPEYLYADNAEELFKEYDFIIDAVDNFETKFLINDTCVLLK
                     KPFCHAGILQFQGQVMTYVPEEDQPCYRCIFEEIPESGSVPNCSQAGIIGAVAGIIGC
                     IQALEAIKYLLGIGELLTGKMLVMDGLTMNTRVVKFPSKNKNCRVCGEHADITSVKEN
                     RMEYVGAACPVQ"
     gene            complement(1240..1494)
                     /locus_tag="PLC71_08565"
                     /pseudo
     CDS             complement(1240..1494)
                     /locus_tag="PLC71_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(1518..2456)
                     /gene="cysK"
                     /locus_tag="PLC71_08570"
     CDS             complement(1518..2456)
                     /gene="cysK"
                     /locus_tag="PLC71_08570"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRM02731.1"
                     /translation="MSKITASALELIGGTPLLKADRYADKAGVKDATILTKLEYLNPA
                     GSVKDRIALAMIEDAEKKGQLKPGSTIIEPTSGNTGIGLAAVATAKGYRAILTLPDTM
                     SVERRNLLKAYGAELVLTEGAKGMKGAIAKADELAKEIDGAVILGQFVNPANPAVHKA
                     TTGPEIWDQTDGKVDIFVAGVGTGGTLTGVGEYLKEQNPDVKIVAVEPATSPVLSKGT
                     AGAHKIQGIGAGFVPDTLNTKIYDEVITIENEDAFTEGRAFAHSEGILVGISSGAALK
                     AATILAERPENKGKTIVALLPDSGDRYLSTALFAEE"
     gene            complement(2644..3462)
                     /locus_tag="PLC71_08575"
     CDS             complement(2644..3462)
                     /locus_tag="PLC71_08575"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM02732.1"
                     /translation="MKNIKVIMCDIDGTLLNSKGIVTSKTIDAIKKVREQGILFGIST
                     GRDVPSVKRLFGKWGIGGLVDMVVGSNGGEIYDYKTDYYEENFALPGNLIANIMEHYK
                     DMDVNFAIHGDGVLYTPKEDELIKILSKGDQLPYEVIDFDEYLKEDRLKLLIVCKPET
                     MPAVIERAKTFKNERFKCASLQTTQHLFEFMDPRISKSFGLQKLMEMHGFTMENLCTF
                     GDADNDYDMTLHAGVGVVMANGSEKTKSVADHITKDNDHDGIGVFLQEHLISRS"
BASE COUNT          937 a          815 c          553 g         1366 t
ORIGIN      
        1 aatacaatca aaaatctgtt tcatataaaa tttgtctttt gaacgtttca tattgtttat
       61 cctttcaaat ttaatgactt tcttttttgc acctcctgtt gtttaacttg aatttagtat
      121 agcacgaaac gattcttcca acttatcaag aacaggcttt ttaatgtggt acattttatc
      181 tattgcaaaa aaaatacggt aaaaattctt ttttgaaatg tggtaatttt taattaaaaa
      241 agaagaactt ctgtttattt tctagttctc cttttgtata aaatataagg gggcagaatt
      301 ctatcttcta ccccttatat ttttctatta atattattgc accggacaag cggccccgac
      361 atattccatc ctattttctt tcacgctcgt aatatccgca tgttctccac agacacggca
      421 gttcttattc ttcgatggaa atttaactac tcttgtattc atcgttaatc catccatcac
      481 aagcatcttg cctgtcagca attccccgat tcctaacaga tacttgatcg cttctaatgc
      541 ctgaatacat ccaatgattc ctgcgactgc accgataatt cctgcctgac tgcagtttgg
      601 cacagaacca ctttctggta tctcctcaaa gatacatctg taacatggct ggtcttcttc
      661 tggaacatag gtcataacct gaccctggaa ctgtaagatt cctgcatgac aaaatggttt
      721 ctttaacaac acacatgtat cattgatcaa gaactttgtc tcaaagttat ccacagcatc
      781 aataataaaa tcgtattctt tgaacaattc ttctgcgtta tccgcatata aatattctgg
      841 ataggtgttt accttgatat gctcatttaa ttcaagtatt gatttctttg ctgattctgc
      901 cttatttgtt ccttctcttt tgcttgtgtg aatgatctgt ctctgtaagt ttgacacgct
      961 gacatcatct gcatccacaa ttccgatcgt tccaacgcct gctcctgcaa gatataaagc
     1021 cgctggtgca cctaagccgc cagctccgat gatcagaact tttgatttct ttaatttctt
     1081 ttgtccgtca cttcctacct gtggcaggat gatctgacga tcatatcgtt taacctcgtc
     1141 tggtgttaat tcttctgtag acgaaaacag atgcagtccg tcctttgaaa cgtcctgcat
     1201 ctgttctttt tgggatgtca catgtaataa ccattctcgg taaccgccgg ataagttcaa
     1261 agcttcatac ccttcttgtc ttaactgttc ggcaattggg gcgcttttct ctccatagtt
     1321 gcaatatacg atcagcctct tatcctttgg aagttcctcg atgtgagaga aatcaccagc
     1381 ccattcgtaa gccttcggta tatgtccatg ctggtatgcg atctcacctc ttacatctag
     1441 gtagatataa tcatcatcgg acagtaattc tacttgctgt accgtgatat ccatattctt
     1501 tcctcttaat cttaatccta ttcttcagca aataatgctg tagataagta tctgtctcct
     1561 gaatctggaa gtaatgctac aattgtcttt cctttgttct ctggtctctc tgcaaggatc
     1621 gtagctgctt ttaatgctgc tcctgaagaa ataccaacta agattccttc gctgtgtgcg
     1681 aatgctcttc cttctgtaaa tgcatcttca ttctcgattg tgataacttc atcataaatc
     1741 tttgtattca gtgtgtcagg tacgaatcca gcaccgattc cctgaatctt atgtgctcca
     1801 gctgttcctt ttgataatac tggagatgtt gcaggttcaa ctgctactat tttaacatct
     1861 gggttctgtt ctttcaagta ttcaccaaca cctgttaatg ttcctcctgt accaacacct
     1921 gctacgaaga tgtctacttt accgtctgtc tgatcccaga tctctggtcc tgttgttgct
     1981 ttgtgtacag ctgggtttgc tgggtttacg aactgtccta agatgactgc tccgtcaatc
     2041 tcttttgcga gttcatcggc tttggcgata gcacctttca ttcctttggc accttctgtt
     2101 aatactaatt cagcaccata ggcttttaat aagtttcttc tttcaacact cattgtgtca
     2161 ggcagtgtaa ggatcgctct gtatcctttt gctgttgcaa ctgctgcaag accgattcct
     2221 gtgttaccac ttgttggttc gatgatcgtt gatcctggtt ttaactgacc tttcttctct
     2281 gcatcttcga tcattgccaa tgcgatacgg tctttgacag atcctgctgg atttaagtac
     2341 tcaagttttg taaggatcgt tgcgtctttc actcctgctt tgtctgcata tctatctgct
     2401 ttaagaagtg gtgttcctcc gattaattct aatgcgcttg ctgtaatctt actcataatt
     2461 ctaaacctcc ataaaaagtt gttgttttta ttccgtttta tttcctattt ttctactagg
     2521 ttttctatgt tttagattat acaaccacat atctttattg tcaacatctt ttgcattatt
     2581 tctgttatag gtttttcttt tgatttgcta taagttctct ttatattttt aatccacaga
     2641 ttcttatgat ctggagatca aatgttcttg cagaaacact ccaattccat cgtgatcatt
     2701 gtctttagta atatgatctg caacactttt tgttttttca cttccatttg ccatgacgac
     2761 tccgactcct gcatgcaagg tcatatcata atcattatct gcatctccaa atgtgcagag
     2821 attttccatt gtaaatccat gcatctccat cagtttttgt aatccaaagg attttgagat
     2881 tctaggatcc ataaattcaa acaggtgttg cgtcgtttga agacttgcac atttgaaacg
     2941 ttcattttta aaggtttttg ctctctcaat aactgccggc atagtttctg gtttgcaaac
     3001 aataagaagt tttaaacgat cttcttttaa atactcgtca aaatcaatga cttcatatgg
     3061 aagctgatca cctttagata aaattttaat aagttcatct tctttcggag tatacaaaac
     3121 tccatcccca tgaattgcaa aattcacatc catatcttta tagtgctcca tgatatttgc
     3181 gatcaggttt cctggaaggg caaaattttc ttcataataa tcggttttgt aatcataaat
     3241 ctcacctccg ttgcttccaa cgaccatatc aacaagcccg ccaattcccc attttccaaa
     3301 caaccttttt acacttggaa catctcttcc tgttgatatt ccaaacaaga ttccttgttc
     3361 tcgaactttc tttatcgcat caattgtttt ggaggtaaca attcccttgc tatttaacag
     3421 tgttccatca atatcacaca ttataacttt tatattcttc ataacgtttc tcccttgatt
     3481 ttgtccataa atattatttt ctaccaattt aacatattct ttcaaactaa ataagtccca
     3541 gaacaaaact ttacgctttt tctgagactt atgaatttta tatatttaga aatcagataa
     3601 caattatttt aattgtaaca tcgcacctcc aaatttttga atcttctgca tcatttttca
     3661 aatcactcct t
//
LOCUS       DAOMUE010000207         3665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7131_length_3665_cov_1.637673, whole genome shotgun sequence.
ACCESSION   DAOMUE010000207 DAOMUE010000000
VERSION     DAOMUE010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7131_length_3665_cov_1.637673"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="PLC71_08580"
     CDS             <1..1433
                     /locus_tag="PLC71_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM02733.1"
                     /translation="FVIHEIEKENSIIGTLYALGVIKKQLLKYYLTVPVIVTFLAGLA
                     GTIIGYSPIGIPTQMQDCYDYFSIPDLSPELLIYLLVYGIVMPPLVAVIVNYFVIRKK
                     LSRPALSMIRNEQKKSHISKVKLGNMPFLTKFRIRQSLREARAGFTVVFGMFIALLVM
                     MIGLDCYVMCDHISKENKKDTKFEYMYTYKYPDKKVPKGGDACFVKGLHKEVWGYDLE
                     ISLIGIENDNPYFSQDKDLPKAKNKVVISSAMAQKYDLKKGDSIILSDEEDEMDYAFQ
                     VADITQYSSGLYAFMDIDSMRDLFQTSSDYYNMVVSKKKLSIDSGKLYATTSKKAIEE
                     SSDVFINMMIPMMGMIVGLSVIIFAIVMYLMIKVMIDRSAQNIALMKVFGYRKKEIKK
                     LYLDGNFYMIAIGALISVPLSKKLMDAMYPVMVSNIACGMNLSFTWQMYALIYAGIII
                     TYLVINKLLVRKIYKVGLSDALKNRE"
     gene            1515..2414
                     /locus_tag="PLC71_08585"
     CDS             1515..2414
                     /locus_tag="PLC71_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM02734.1"
                     /translation="MTLQQLRYVVTIAEIGTISKAAEELFVSQPSLTKALKELEKEMQ
                     ITIFDRTNKGIHVSREGEIFLGYARQVLEQAALIEEKYKHKSGGKQEFCISTQHYSFA
                     VNAFVDLIKEYGSENYDFSLRETQTYEIIDDVARMKSEIGILYLNEFNASVLEKIMKA
                     NDLKFTELFVARPHIFICDKNPLAQKDQVTMEDLKDYPYLSFEQGEHNSFYYSEEMFS
                     TEVRSKNIRVRDRATLFNLLIGLNGYTISSGVIDKELNGENIIAVPLAEEGEMHIGYV
                     THKKTKPSRLGAIYLEALKRHAQ"
     gene            2535..>3665
                     /locus_tag="PLC71_08590"
     CDS             2535..>3665
                     /locus_tag="PLC71_08590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HRM02735.1"
                     /translation="MITGELKNKVDKIWEIFWTGGITNPLEVIEQFNYLLFIKQLDEV
                     ETRKERDANILGIPYEGIFPADCQQYRWSKFKNLGDANEIYDLMMNGVFPFIKSLHPD
                     GESAYSKYMGDAIFKIPTPALLTKVIDGIDGLNLEGDSKGDLYEYLLSKLESAGKNGQ
                     FRTPRHIIQMMVELVKPVSSDIICDPAMGSAGFLMAAQQYLRKNHKDLFLNAEQREHF
                     NHEMFYGFDMDRTMLRIGAMNMLLHGVDDPNIEYKDSLSEMNTDKEKYSLILANPPFK
                     GSLDYDGVSADLLKVAKTKKTELLFLVLFLRIMKIGGRAAVIVPDGVLFGSSRAHKAI
                     RKELIENHKLDAVISMPSGVFKPYAGVSTAILLFTKTGAGGTD"
BASE COUNT         1288 a          519 c          806 g         1052 t
ORIGIN      
        1 tttttgtcat tcatgaaatt gagaaagaaa atagtattat tggaacattg tatgcgcttg
       61 gagtgataaa gaagcagctt ctaaaatatt atttaacagt tcctgtgatc gtaacatttc
      121 tggctggatt agcaggaacg atcattggat atagtccgat aggaattcca acacagatgc
      181 aggattgtta tgattatttt tctataccag atttgagtcc agaactttta atctatctat
      241 tggtgtatgg aattgttatg ccgccacttg ttgcagtgat cgtaaactat tttgttataa
      301 gaaagaaact gtcaaggcca gcactttcta tgataagaaa tgagcaaaag aagagtcata
      361 tttcaaaagt caaattaggc aatatgccat ttcttacaaa attcaggatc aggcagtcac
      421 tgcgtgaggc aagagccgga tttacggtag tatttggaat gtttattgca ctgttggtta
      481 tgatgatcgg tctggactgt tatgtgatgt gtgatcatat cagcaaagaa aataagaaag
      541 acacaaaatt tgaatatatg tatacatata aatatccaga taaaaaagta ccaaaaggcg
      601 gggatgcatg ttttgtaaaa ggattgcata aagaagtatg gggatatgat cttgaaattt
      661 ccctgatcgg tatagaaaat gataatccat atttcagcca ggataaagat cttccgaaag
      721 caaagaacaa agttgttatt tcatctgcaa tggcacagaa atatgatcta aaaaaaggtg
      781 actcgatcat actgtctgat gaggaagatg aaatggatta cgcatttcag gtagcagata
      841 tcacacaata ttcttctggg ttatatgcat ttatggatat cgacagcatg agagatctct
      901 ttcagacatc atcagattat tacaatatgg ttgtatcaaa gaagaaactg tcgattgact
      961 ctggaaaact atatgcaacg acaagtaaaa aagcaattga ggagagttca gatgtattta
     1021 tcaatatgat gattccgatg atgggaatga tcgttggact ttctgttatt atctttgcaa
     1081 ttgtcatgta tttaatgatc aaggtaatga ttgatcgttc tgcacaaaat attgcgttga
     1141 tgaaagtatt tgggtatcgg aagaaagaga ttaagaagct ttatctagat gggaatttct
     1201 atatgatcgc aattggagca ctgatcagtg ttccattatc caagaaatta atggatgcaa
     1261 tgtatccagt tatggtttcc aatatagctt gtggtatgaa tttaagtttc acatggcaga
     1321 tgtatgcatt gatctatgca ggaattatca ttacatacct ggtaattaac aagttattag
     1381 tgcgaaagat ttacaaagtc ggactttctg atgcgttaaa gaatcgtgag taagattaaa
     1441 agaaacaatc agtctttgcc atataaaata aactattgta taatgataca aaaagaccaa
     1501 agaaggaaga aacgatgaca ttacaacagc taagatatgt cgtaacgatt gcggagatcg
     1561 gaacgatcag caaggcggca gaagaacttt ttgtttcaca gccaagtctt accaaagctc
     1621 taaaagaact ggaaaaagag atgcagatca cgatcttcga ccgcacgaac aagggaattc
     1681 atgtatccag agaaggagag attttcctcg gatatgcaag acaagtactg gagcaggcgg
     1741 cattgatcga agagaaatat aaacataaaa gtggtgggaa acaggaattt tgcatttcaa
     1801 cacagcatta ttcttttgca gtcaatgcat ttgtagactt gatcaaagaa tatggaagtg
     1861 agaattacga tttcagtctg agagaaactc agacttatga gattatcgat gatgtagcca
     1921 gaatgaaaag tgagattgga attttatatc tcaatgaatt caatgcatcg gtccttgaga
     1981 agatcatgaa agcaaatgat ctcaaattta cggaactatt tgttgcaaga ccacatattt
     2041 ttatctgcga taagaatccg ctggcacaga aagatcaggt cacaatggaa gatctcaaag
     2101 attatccgta tctgtcattt gagcaggggg aacataattc tttctattat tctgaagaga
     2161 tgttcagtac ggaagtgcgt tcaaagaaca ttcgagtgag agacagggca acgttgttta
     2221 acttgctgat cggattaaat ggatatacga tcagcagcgg tgtgatcgat aaggaattaa
     2281 atggagagaa tattatcgca gttccgcttg cagaagaagg cgagatgcat attgggtatg
     2341 tgacacataa gaagacgaaa cctagcagat taggggcgat ttacttggaa gcgttgaaga
     2401 ggcatgcaca ataattttat atatgtttaa agaaaggtta taactgtatc atggtagaga
     2461 tacaggttgt gaccttttgt gattttatgg tataattttc acataaaaaa cgaaaatgaa
     2521 gaagaggaag taagatgatt acgggtgaat taaaaaacaa agtagataag atatgggaga
     2581 ttttctggac aggtggaatt acaaatccac tggaagttat tgaacagttt aattacttac
     2641 tatttatcaa gcagttagat gaggtagaaa ccaggaaaga aagagatgcg aatattttag
     2701 ggattccata tgaaggaatt tttccagcag attgtcagca gtatcgttgg agtaaattta
     2761 agaatttggg agatgcgaat gagatttatg atctgatgat gaatggagta tttccgttta
     2821 ttaaaagtct acatccagat ggagaatctg cttatagtaa atatatggga gatgcgatct
     2881 ttaagattcc aacgccagca ttacttacca aagtaattga tgggattgat ggattgaatc
     2941 tggaaggaga cagtaaagga gatttatacg aatatctgtt atccaaactg gaaagtgcag
     3001 gaaagaacgg acaatttcga accccaagac atatcattca gatgatggta gaattggtaa
     3061 aaccagtatc atctgatatc atctgcgacc cggcaatggg atcagcagga ttcttaatgg
     3121 cagcacagca gtatttgcgt aaaaaccaca aagatttatt cttaaatgcg gaacaaagag
     3181 agcattttaa tcatgagatg ttctatgggt ttgatatgga ccgaacgatg cttcgaatcg
     3241 gagcaatgaa tatgctgctt catggagtcg atgatccgaa tattgaatac aaagacagct
     3301 tgtcagagat gaatacagat aaagaaaagt attcattaat tcttgccaat ccaccattta
     3361 aaggaagttt agactatgat ggagtatcag cagatctttt aaaagttgca aaaacaaaga
     3421 aaacagaatt attattcctt gttttattcc ttcgtatcat gaaaattgga ggaagggcag
     3481 cagttatcgt accagatgga gtattattcg gaagcagtcg tgcacataaa gcgattcgaa
     3541 aagaattaat tgaaaatcat aaattagatg cagtgatttc catgccatct ggagtattta
     3601 aaccatatgc aggagtatcc acagcaatct tactgttcac aaagacaggg gcaggtggta
     3661 cggat
//
LOCUS       DAOMUE010000208         3632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7234_length_3632_cov_1.859938, whole genome shotgun sequence.
ACCESSION   DAOMUE010000208 DAOMUE010000000
VERSION     DAOMUE010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7234_length_3632_cov_1.859938"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /gene="hemA"
                     /locus_tag="PLC71_08595"
     CDS             complement(<1..880)
                     /gene="hemA"
                     /locus_tag="PLC71_08595"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRM02736.1"
                     /translation="MQFGFLGIDYKNADLTIRDEISFTDQKKLEFFHKAEEIGVEQVM
                     ILSTCNRSEIYYFFDDEQQIKKIQNIYCDMFDKAEIEQYIRHCEEDKAVSYLFQVTAG
                     LESMVLGEDQILGQVKDALDFSRTMGFSKKELNKVVRDAITCAKKVKTTFRISEKPVS
                     VGYIGICELQKICDIKDKMVLVIGSGDTAVLALRYLQEYEAGKIYLCSRTLAHAGNVQ
                     KEFQEIEIISYEQRYEIMKQCDIVVSATSAPHVVVKQEYYTPEKQVTFLDLATPRDID
                     PKLSDDSKVNLINLDTI"
     gene            complement(862..2145)
                     /gene="hemL"
                     /locus_tag="PLC71_08600"
     CDS             complement(862..2145)
                     /gene="hemL"
                     /locus_tag="PLC71_08600"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRM02737.1"
                     /translation="MKDTRSKELYERAKVHIPGGVNSPVRAFRAVNRTPRFMESAKGD
                     RIIDADGNEYIDYVCSWGPGILGHAHPQVIKKVMDSCENGLTFGAPTEKEVILAELIS
                     ELIPSMEVSRLVSSGTEAVMSAIRAARGFTGRDKIVKFRGCYHGHSDSLLVKAGSGAL
                     TTSVPDSAGVPKDDTKNTLVAEYNDKDSVQKLFDENKDQIAAVIVEPVAANMGLVLPE
                     EGFLEFLREITKDHGSLLIFDEVITGFRLSLGGAQQYYNIKPDITTLGKIVGGGMPIG
                     AYGGRREIMQMISPDGPVYQAGTLSGNPVATTAGIETLNILKNDPQIYERLEQKTRKL
                     ADVAREAGKGHICVNQIGSLMSVFFTDQKVRDFESAVTSNTEQYTDYFGYLLDRGIYI
                     APSQFETMFISNAHTEEDIEKTCKLAGEALCSLDF"
     gene            complement(2147..3118)
                     /gene="hemB"
                     /locus_tag="PLC71_08605"
     CDS             complement(2147..3118)
                     /gene="hemB"
                     /locus_tag="PLC71_08605"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRM02738.1"
                     /translation="MERTRRLRTSNYMRDMVRENHVRIDELIYPIFVTEGENIKHPVE
                     SMPGIYQYSLDRLSEEINRIKEAGIKAILIFGIPDHKDEVGSGAYAEDGIVPKAIRQI
                     KKEYEELLIIADVCLCEYTSHGHCGLVKDGVILNDETLPLLAKASVSYAKAGADIIAP
                     SDMMDLRVKAIREALDEAGFVYTPIMSYSAKFASGYYGPFRDAAHSAPGFGDRKTYQM
                     DPANGQEALREIGEDIEEGADLIIAKPALAYMDIMKEASMKYNIPIVAYNVSGEYAMV
                     KAAAANGWIDEKKIVMENMVGLKRAGAKMIITYHALDVAKWLKEEER"
     gene            complement(3137..>3359)
                     /locus_tag="PLC71_08610"
     CDS             complement(3137..>3359)
                     /locus_tag="PLC71_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HRM02739.1"
                     /translation="TQYLKEKIKPDLNKELGQLAQILGSLRKLAKSCIATEQERKAFY
                     KELLQIGLEDIDSLEEQRINEIIQKYISE"
     assembly_gap    3360..3369
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3370..>3632)
                     /locus_tag="PLC71_08615"
     CDS             complement(<3370..>3632)
                     /locus_tag="PLC71_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM02740.1"
                     /translation="IAPEMITQIRQIDQICRIFRTFMDKDFNEADFVIAATDDQKTNH
                     EISQICRRKKIPVNAVDQKEDCSFIFPSYVKEGEVVAAFSSGG"
BASE COUNT          938 a          823 c          562 g         1299 t
ORIGIN      
        1 tgatcgtatc caagttgatc agatttacct ttgagtcatc tgaaagtttt ggatcaatat
       61 ctcttggtgt tgcaagatct aaaaatgtaa cttgtttttc tggcgtataa tattcttgtt
      121 ttacaaccac atgtggtgct gatgttgcag atactacaat atcacattgt ttcatgatct
      181 catatctttg ttcataagaa atgatctcaa tctcttgaaa ctctttctga acatttcctg
      241 catgtgccag tgttctgctg cataaataaa tcttgcctgc ttcatattcc tgcagataac
      301 gaagtgcaag aactgcagta tctccgcttc cgatcaccaa gaccattttg tctttgatat
      361 cacatatttt ctgtaattca cagattccga tatatccaac agatactggt ttctcactta
      421 ttctaaacgt tgtttttacc ttttttgcac aagtaatagc atctcttacg actttattta
      481 gttctttctt gctaaatccc atggttcttg aaaaatccaa ggcatctttt acctgtccaa
      541 gaatctgatc ttctcctagt accatagatt ctaatcctgc ggttacttga aacagataag
      601 aaactgcttt atcttcttcg caatgtctga tatattgctc gatctctgct ttatcgaaca
      661 tatcacagta tatattttgt atttttttga tctgttgttc atcatcaaaa aaataataaa
      721 tctcactgcg gttacaggtc gataagatca tcacctgttc aaccccgatt tcctctgctt
      781 tatgaaaaaa ttcaagcttc ttctgatccg taaatgaaat ttcatcacga atggtaagat
      841 ctgcattttt ataatcaatt cctagaaatc caaactgcat agagcctctc ctgcaagttt
      901 acatgttttc tcaatatctt cttctgtatg tgcgttagaa ataaacattg tctcaaactg
      961 agatggtgca atataaattc cacgatctaa taaatatcca aaataatctg tatactgttc
     1021 tgtattggat gtcacagcac tttcaaaatc tctgactttc tggtctgtaa agaatacact
     1081 cattaaagat ccaatctgat taacacaaat atgtcctttt cctgcttctc ttgctacatc
     1141 agcaagtttt cttgtttttt gctctagtct ttcgtaaatc tgcggatcat tttttaagat
     1201 atttaatgtc tcaattcctg ccgttgttgc aactgggttt cctgacaatg ttcctgcctg
     1261 ataaactggc ccatctggag agatcatctg catgatttct cttcttccgc catacgcacc
     1321 gatcggcatt cctccaccaa caatttttcc aagtgttgtg atatccggct tgatattata
     1381 atactgctgt gctcctccta gagatagcct aaaacctgta ataacctcat caaagatcaa
     1441 taatgatccg tgatcttttg taatctctct taaaaattcg agaaatcctt cttctggaag
     1501 tactaatccc atattagctg ccaccggttc tacgatcact gctgcgatct gatctttatt
     1561 ttcatcaaat agtttttgta cagaatcttt gtcattgtat tctgcaacta atgtattctt
     1621 cgtatcatct tttggaactc cggcactgtc tggaacggat gttgtaagtg ctcccgatcc
     1681 tgcttttact aacaaactgt cggaatgtcc atgatagcat ccgcggaatt taacgatctt
     1741 atctctccct gtaaaccctc tggctgcacg gatcgcactc ataacagctt ctgttccaga
     1801 acttaccaga cggcttactt ccatagacgg gatcaattca ctgatcaatt ctgcaaggat
     1861 cacttccttc tctgttggtg ctccgaaagt caaaccgttt tcacaagagt ccatgacttt
     1921 tttgatgact tgaggatgag catgtcctaa gattccaggt ccccatgaac atacataatc
     1981 gatatattca tttccatcgg cgtcaataat acggtctcct tttgcagatt ccatgaatct
     2041 tggagtacga tttaccgcac ggaatgcacg aactggactg tttacccctc ctggaatatg
     2101 tacttttgct cgttcatata attcttttga tcttgtatct ttcattttat ctttcctctt
     2161 ctttcagcca ttttgcaaca tcaagtgcat gatatgtaat gatcatcttt gcaccagcac
     2221 gttttaatcc gaccatattt tccataacga tctttttctc atcgatccat ccattggccg
     2281 cagctgcttt taccatagca tattctccgc ttacattgta tgcaacgatc ggaatattgt
     2341 atttcataga tgcttctttc ataatatcca tataagcaag tgctggtttg gcaatgatca
     2401 agtctgctcc ttcttcgatg tcttccccga tctctcttag agcttcctgt ccatttgccg
     2461 gatccatctg gtatgttttt ctatctccaa atcctggtgc agaatgtgct gcatcacgaa
     2521 atggtccata gtatcctgac gcaaacttgg cactataaga catgatcggt gtgtatacaa
     2581 atcctgcttc atctaaagct tcacgaatag ctttcactcg aagatccatc atatcacttg
     2641 gcgcaatgat atctgcccct gcttttgcat aacttacaga tgcctttgca agaagcggaa
     2701 gtgtttcatc atttaagatc actccgtctt tcacaagacc acagtgtcca tgagatgtgt
     2761 attcacaaag acatacatcc gcaatgatca aaagttcttc atactctttt ttgatctggc
     2821 ggatcgcctt tggcacaatt ccatcttcag catatgcacc gcttccaact tcatctttat
     2881 gatctgggat tccaaagatt aaaattgctt tgatccccgc ttctttgatc ctgttaattt
     2941 cctctgataa acgatccagc gaatactgat aaatccctgg catagattct acaggatgtt
     3001 tgatattttc tccttctgta acaaagattg gatagatcaa ttcgtcaatt ctcacatgat
     3061 tttctcttac catatctctc atatagttgc ttgttcttaa tcttcttgta cgttccattt
     3121 ttactgttcc tcgatttcac tctgatatat atttttgaat gatttcattg attcgttgtt
     3181 cttccaatga atcaatatcc tctaatccga tttgtaataa ttctttataa aatgcctttc
     3241 gttcctgttc tgtggcaatg catgattttg caagctttct cagacttccc aagatctgtg
     3301 cgagttgtcc taattcttta ttcaaatctg gtttgatctt ttcttttaaa tactgtgtgn
     3361 nnnnnnnnnc ccgccacttg aaaatgcagc gacaacttct ccttctttta cataggatgg
     3421 aaaaataaag ctgcaatctt ctttctgatc gaccgcattg acaggaatct tctttcgtct
     3481 gcagatctgg ctgatctcat gatttgtttt ttgatcatcg gttgctgcga tcacaaaatc
     3541 tgcctcatta aaatctttat ccataaacgt tcgaaagatt cggcaaatct gatcgatctg
     3601 ccgaatctgt gttatcatct ctggtgcgat ca
//
LOCUS       DAOMUE010000209         3626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7248_length_3626_cov_2.246990, whole genome shotgun sequence.
ACCESSION   DAOMUE010000209 DAOMUE010000000
VERSION     DAOMUE010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7248_length_3626_cov_2.246990"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLC71_08620"
     CDS             <1..727
                     /locus_tag="PLC71_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02741.1"
                     /translation="DVHYPLQSEIQNEIEDIILKGMPQKISFLKRMKKIYLGPGLRVI
                     YYKNLSVFFATFIIYFAVCLGLVLSFEEEIRQFGAAFLGMPVFFLLFSYFTCYMDEQT
                     QINELRNTMYYSMNYIVSLRLFYTAVVLSLVNTSFLFLSGIREKAILSVGVVGVSSML
                     VFAIIAVYIYHRWNNYKCFAGLGAIWVLLFAAIMRLPETKILFLFEYIPLGIQIATMI
                     VCFVALFTLVGRMEEKDAYTFAY"
     gene            705..1565
                     /locus_tag="PLC71_08625"
     CDS             705..1565
                     /locus_tag="PLC71_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM02742.1"
                     /translation="MLTLSHIRKDYGKFTAVEDINLELENGLYAMLAPNGAGKTTLIK
                     MITTLLYPTSGEILYDGMDIYKMGETYRDVIGYLPQHFGYYKNNTPTQYLDYLAALKG
                     ISKEAAREKIPELLELVGLSDVFNKKMKKFSGGMIQRVGIAQAMLNDPKILVLDEPTA
                     GLDPKERVRFRNILSVLSKDRIVILSTHIVSDIESIANHVIMIKEKQLLKNDTVPNIC
                     KELEGKVFEKSINESEIQEFEQKRIILSMRQESEKMMVRYYSEEEDNNARPCTPNLED
                     VFLVTYREEK"
     gene            1567..2772
                     /locus_tag="PLC71_08630"
     CDS             1567..2772
                     /locus_tag="PLC71_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02743.1"
                     /translation="MRIIINECKKILDIRIILLLCMFSILYYMTFTQIYRYPTGGQVT
                     NTKYDIPFTAELVKEWGPKWKVKDEKQYQEKHQELERGFAKIIKQDQELSKRGIVDYE
                     IFNKKYEELGQKTTLSKQEKELGKILENYVFYNDKTSKIFLDIQALEHIREFQGSEYG
                     VSDKKYKELKADGFFDGTKLYQKAIEKRVKRDYISLVHEGVFYILQEDMVTIGGLIMI
                     CFFALIIPYQLKQRLRQVIPILATTKTGRRIYQIQLIASALAALFVGILQMAVYGIIW
                     HLKGLSVFWRCESWGIASNSYWCDKLSFGTYMLLYMALILLFAIASIVIIDFIGRTIG
                     NYMGAVAVSIPVCGVIMFLMRKIYYMLFLITNDQVLAYWELYALATWLIVMFIFYKIR
                     SNRWKKVDL"
     gene            2769..>3626
                     /locus_tag="PLC71_08635"
     CDS             2769..>3626
                     /locus_tag="PLC71_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02744.1"
                     /translation="MRIFKLEFQKIMKNPMLWLLAVIFCLVNCLIIYNEAGNKDTSKE
                     LVIMHDIIKKTDDTNHSRQAKNRNERKQLSEAYQEYYKENIDLYNKLDMTKILKQKEE
                     MFQYHPKGFMKEFVKDNYEHLQKRVEEIKSDKEYNDAFYPGQYYEIHSLLYSKLGKKL
                     IVEMSLLMALSILFIMDYERLQKTNDLVDATRTGKRIMDYKAFTGTLSGILFSAILCS
                     VTWIYFFYCVSFKGLWNVSVASTLVAEKRYSGWFYPFVTFFKMTQMQYLILTLTVYLG
                     IILLIALATI"
BASE COUNT         1266 a          491 c          739 g         1130 t
ORIGIN      
        1 ggatgttcat tatccgctgc agtcagagat acaaaatgaa attgaagaca tcattttaaa
       61 aggtatgcct caaaagattt catttctaaa aagaatgaaa aagatttatc tgggacctgg
      121 attaagagtc atttattata aaaatctttc tgtatttttt gcgacgttca taatttattt
      181 tgccgtgtgt cttggactag tactttcctt tgaggaggaa atcagacaat ttggggcagc
      241 ttttttagga atgcccgttt tctttctgtt gttttcttat tttacttgct atatggacga
      301 acagacgcag atcaatgaat taagaaatac gatgtattat tcgatgaatt atattgtgag
      361 ccttcgccta ttttatacag cagtcgtatt atctctggtg aataccagct ttttattcct
      421 tagtgggatt agggagaaag caatcttatc ggttggagtt gttggagttt caagtatgct
      481 ggtctttgcg atcattgctg tatacattta tcacagatgg aataactata agtgctttgc
      541 gggtcttggt gcaatatggg tattactttt tgcagcgatc atgcgattac cggaaacaaa
      601 gattttattt ctttttgaat atattccatt aggaattcag attgcaacaa tgatcgtgtg
      661 ttttgtggct ttatttactt tggtcggaag aatggaggag aaggatgctt acactttcgc
      721 atattagaaa agattatgga aaattcacag cagtggaaga tattaatctg gaacttgaaa
      781 atggtttata tgccatgctc gcaccaaatg gtgcagggaa aacgacattg atcaaaatga
      841 tcacaacact tttgtatccg acaagcggag agattttata cgatggaatg gatatttata
      901 aaatgggaga aacataccgg gatgtgatcg gttatttgcc acagcatttt ggatattata
      961 aaaataatac accaacgcag tatcttgatt atcttgcagc gttaaaagga atttccaaag
     1021 aagctgcaag agaaaaaatt ccagaattat tggagttagt aggattaagc gatgtcttca
     1081 ataagaaaat gaaaaaattc tctggtggaa tgattcagcg tgtagggatc gcacaggcaa
     1141 tgttaaatga tccaaaaatt cttgtattag atgaaccaac tgcaggactt gatccaaaag
     1201 agagagtaag gtttcgaaat atattatccg tattatccaa ggatcgaata gtgatccttt
     1261 caacgcatat cgtatcagac attgaatcta ttgcaaatca tgtcattatg atcaaagaaa
     1321 aacagttatt aaaaaatgat acagttccaa acatctgcaa ggagcttgag gggaaggtat
     1381 ttgagaaatc aatcaatgaa tccgagatcc aagaatttga acaaaaacga atcattttat
     1441 ccatgcgaca ggaatcagaa aaaatgatgg tccgatatta tagtgaggaa gaagataata
     1501 atgcaagacc gtgtacacca aaccttgaag atgtattttt agtcacttac agggaggaaa
     1561 aataacatgc ggatcataat caatgaatgt aaaaaaatat tggatattcg aatcatactg
     1621 ttattatgta tgttctctat tttatactat atgacattta cacaaatata tcgatatcca
     1681 actggaggac aggtaacaaa tacaaagtat gacattccat ttacggcaga actagtgaaa
     1741 gaatggggac caaagtggaa agtaaaagat gaaaaacaat accaggagaa acatcaggaa
     1801 ttagaaagag gttttgcaaa gatcataaaa caagatcaag agctttcaaa aagagggatt
     1861 gtggattatg aaatttttaa taaaaaatat gaagaacttg gtcaaaaaac aactttatca
     1921 aagcaggaaa aagaacttgg taaaatatta gaaaattatg tgttttataa tgataagaca
     1981 tcaaaaattt ttttggatat acaagcttta gaacatatca gagagtttca aggatctgaa
     2041 tatggagttt cggataaaaa atataaagaa ttaaaagcag atggattttt tgatggaacg
     2101 aagttatatc aaaaggcaat cgaaaagagg gtaaaaagag attacatatc attagtacat
     2161 gagggagtgt tttacatttt gcaagaagat atggtaacga tcggtggttt gatcatgatc
     2221 tgtttttttg cattgatcat tccatatcag ctaaaacaac gtttaagaca ggttatcccg
     2281 attctggcaa cgacgaaaac aggaagaagg atttatcaga ttcaattgat agcaagtgca
     2341 cttgctgcac tctttgttgg aattcttcaa atggctgtat atgggattat ctggcatttg
     2401 aagggattat cggtattttg gagatgtgag agttggggaa tcgcatcaaa ttcttactgg
     2461 tgcgataagc tttcttttgg aacttatatg ttactttata tggctctgat cttattattt
     2521 gcaatcgcaa gtattgtgat cattgatttt atcggcagaa caattggaaa ttatatggga
     2581 gctgttgcgg tttcaattcc agtatgtggg gtgataatgt ttcttatgag gaaaatttat
     2641 tatatgctct ttctgatcac aaatgatcag gttctggcat actgggaatt gtatgcacta
     2701 gccacatggc tcatagtgat gtttatattt tataagatca gaagtaatcg atggaaaaag
     2761 gtggatttat gagaattttt aaattagagt ttcaaaagat catgaaaaat cctatgcttt
     2821 ggttattggc agtgatattt tgtcttgtga attgtttaat tatttacaat gaggcgggaa
     2881 ataaggatac atccaaagaa ttagtgatca tgcatgacat tatcaaaaaa acagatgata
     2941 caaaccactc acgtcaagct aaaaatagaa atgaaagaaa gcagttatcc gaggcatacc
     3001 aagaatatta caaagaaaat atcgacttgt ataacaaact tgatatgacg aagattttaa
     3061 aacaaaaaga agaaatgttt cagtatcacc ctaagggatt tatgaaggaa ttcgtaaaag
     3121 ataactatga gcatctgcaa aaaagagtgg aggagatcaa atctgataaa gaatataacg
     3181 atgcttttta tccaggacag tattatgaga ttcattcgtt attatattca aagttaggga
     3241 aaaaattaat cgtggaaatg agtcttttga tggcactcag tattttgttt atcatggatt
     3301 atgagagact gcaaaagaca aatgatcttg tagatgcaac cagaactggg aagagaatca
     3361 tggattataa agcttttacg gggacgttaa gtggaatttt attttcagcg atactatgca
     3421 gtgttacatg gatttatttt ttttactgcg tatcattcaa aggactatgg aatgtctcgg
     3481 ttgcctccac actagttgca gaaaaaagat attcgggatg gttttatcca tttgttacat
     3541 tctttaaaat gacgcagatg caatacttga tcctaacact taccgtatat cttggaatca
     3601 tcctgttgat tgcactggca acgatc
//
LOCUS       DAOMUE010000210         3575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7391_length_3575_cov_2.905966, whole genome shotgun sequence.
ACCESSION   DAOMUE010000210 DAOMUE010000000
VERSION     DAOMUE010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7391_length_3575_cov_2.905966"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLC71_08640"
     CDS             complement(<1..251)
                     /locus_tag="PLC71_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HRM02745.1"
                     /translation="MDGRIFGLDIQLGFDVIMQGIAVFIMFALLSYILFEPVRKILED
                     RKNRIADEIDQAAADQAEAAKLKAEYDHKLKNVEKEADA"
     gene            complement(279..542)
                     /gene="atpE"
                     /locus_tag="PLC71_08645"
     CDS             complement(279..542)
                     /gene="atpE"
                     /locus_tag="PLC71_08645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRM02746.1"
                     /translation="MNISSEAFVLGCSAIGAGLALIAGIGPGVGQGYAAGQGAAAVGR
                     NPGAKGDITSTMLLGQAVAETTGLYGLVIGLILLYANPLFAKL"
     gene            complement(585..1316)
                     /gene="atpB"
                     /locus_tag="PLC71_08650"
     CDS             complement(585..1316)
                     /gene="atpB"
                     /locus_tag="PLC71_08650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRM02747.1"
                     /translation="MGIMNAPMMLAASSGDDVDFMVHGLIPLKFFGHKVWITTTHVTT
                     LIVMVFIIVLALIARHAVLHGDEKPSGLQNFVEMIVEMLDNLVKGSMGRHWRPFVNYI
                     GTIMVFIFFANTSGLFGLRPPTADYGTTLALALITFVLIQYSAFKTSKLHVFTDLFKP
                     IPILFPINLIGEFATPLSMSLRLFGNIMAGTIMMALWYGLLPWLAQLGIPAFLHMYFD
                     LFSGAIQTFVFAMLTMTFITDKREA"
     gene            complement(1400..1804)
                     /locus_tag="PLC71_08655"
     CDS             complement(1400..1804)
                     /locus_tag="PLC71_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HRM02748.1"
                     /translation="MRDKLRQLDETYLDLVIGILLCGVLSAILGMIITRGDLRYLIGA
                     IVGTIFALVMITTMMVSIQKSVQMDTHQASRNMIKSALIRYVATLVILYVMIKLSMSA
                     FIAALVGFLSSKFAAFLQKPIHEHITRRVLQE"
     gene            complement(1794..2030)
                     /locus_tag="PLC71_08660"
     CDS             complement(1794..2030)
                     /locus_tag="PLC71_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRM02749.1"
                     /translation="MHKYGPVIKMLSLVTQIGITMLTSVFLCMAIGMWIDKHFSTNLF
                     LLFLILGIMGGIRGVYSLIHNILKQEDMEDKDER"
     gene            2384..3553
                     /locus_tag="PLC71_08665"
     CDS             2384..3553
                     /locus_tag="PLC71_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HRM02750.1"
                     /translation="MPLTLNELYTQTKHQYHLSMIAGETGIDHIMNWVYVSEDSSTHD
                     FLKGGELIITTGINCQDEASLYDFIETMIKSHTCGMILNTGKYILSEDITDSIKALCD
                     KHNYPLIEMPWKVHIYDITRDYYNKIFMDTQKDTSITDAFLSFIEEDSLHHYDALRIL
                     EDNHFEKEDCYEIALMKCNKKAISDDLKLRLLFLMESYIKLNDLDIHIAFYKNHFLFV
                     FHDMEEELIYNEISSLISTLKSSFKKMQIYAGIGSSVHTLREIRVSYQRSLAALVYAA
                     NNGQEIARFEEMGFFRILHSVNDKNLLMAYHDEYLKNIEEYDEIHDTNYLETLHQYLL
                     CNGSIQHVASNMFCHRNTITYRMRVIEDHWNLKLDDTVERFHLMTAFFIRDYLNL"
BASE COUNT         1083 a          729 c          607 g         1156 t
ORIGIN      
        1 aaggcatccg cttctttttc aacatttttt agtttatgat cgtattcggc ttttaacttt
       61 gcagcttctg cctgatcggc tgctgcctga tcgatctcat ctgcaattct gttcttacgg
      121 tcttctaaga tcttgcgaac cggttcaaac agaatatagg aaagcagtgc aaacatgatg
      181 aacactgcga ttccctgcat aatcacatca aagccaagct gaatatctaa tccaaatatt
      241 cttccgtcca cgccttggcc tcccttcggc ttgttttatt aaagttttgc aaacagtgga
      301 tttgcatata ataagatcaa accaataacc agaccataaa gacctgttgt ctctgctacc
      361 gcctgtccaa gaagcatggt agatgtgata tcacctttcg ctcctggatt acgtcctact
      421 gctgctgcac cctgtcctgc tgcgtatccc tgtccaacac caggtccgat acctgcaatc
      481 agtgcaagtc ctgctccgat cgcagaacac cctaatacaa atgcttcact tgaaatattc
      541 atagtttttt cctcctaaac tacataaaat tttgtcgcta aaatttaagc ttcccgctta
      601 tctgtgataa atgtcattgt cagcatcgca aatacaaatg tctggatcgc tcctgaaaac
      661 agatcaaagt acatatgcaa gaatgctgga atacctagct gcgcaagcca tggcagcaat
      721 ccgtaccaca atgccatcat gatcgtaccc gccataatat ttccaaacaa acgaagagac
      781 atggaaagcg gtgtcgcaaa ctctccgatc aggttaattg gaaataagat tgggatcggt
      841 ttgaaaagat ctgtaaatac atgtaattta ctcgtcttaa atgcactgta ctgaatcagt
      901 acaaacgtga tcaacgctaa cgcaagcgtt gtcccataat ccgctgtcgg tggtcttaag
      961 ccaaataatc cagatgtatt tgcaaaaaag ataaagacca tgatcgttcc aatgtaattt
     1021 acaaatggac gccaatgtct tcccatgctt ccctttacca gattatcaag catctcaacg
     1081 atcatctcca caaaattctg aagtcctgac ggcttctcat caccgtgaag aactgcatgt
     1141 cttgcgatca atgctaatac gatgataaac accataacga tcagcgtcgt cacatgcgtt
     1201 gtcgtgatcc agaccttatg cccgaaaaac ttcagcggga tcagcccatg caccataaag
     1261 tcaacgtcat caccggatga tgctgccaac atcatggggg cattcataat tcccatggca
     1321 caacctcctt tttattatat tttccatttc tccgattttc tctttctcca tgttagaaac
     1381 ccggaaaatg tgattcacgt tattcttgta aaactctgcg cgtgatatgc tcgtgaatcg
     1441 gtttttgtaa aaatgctgca aacttactgc ttaagaatcc aaccaatgca gcaataaaag
     1501 cactcatact aagtttgatc atcacgtata aaataaccaa tgtcgctaca tatcttatca
     1561 acgcactctt gatcatattc cggcttgcct gatgagtatc catctgtacg gacttctgga
     1621 tgcttaccat catcgttgtg atcatcacga gagcaaagat cgttcccaca atagctccga
     1681 tcagatatct tagatcacct ctggttatta tcatacccaa aatcgcagat aacactccgc
     1741 acaaaagaat tccgatcaca agatctaggt atgtctcgtc tagctgtctt aatttatctc
     1801 tcatctttat cctccatatc ttcctgtttt agtatattgt gaatcagaga atatactcct
     1861 ctgattcctc ccataatacc aagaatcaga aaaagaagaa atagatttgt ggaaaaatgt
     1921 ttatcaatcc acattccgat cgccatgcat aaaaaaacag aagtcaacat cgtaattcct
     1981 atctgagtca ccaatgacaa catcttgatc actggtccat acttatgcat agatatttcc
     2041 tccttcttgc gaaatctttt tacaaaaatt attataacat tttttacaaa tgcaaaccac
     2101 catatttttt gtttcttatc tatttttgcc aaaaataacc agagaaattg aatttttcat
     2161 caatttttct ctggtttctt cttattaaat agttatttat ttttttgaat aatttttttc
     2221 atttttgtgt gatcattcca ccaatttttt attagtttcc tatattcttt tatcacaatt
     2281 ttcctttgat tctttgtgtt cattgcacaa atagtatcaa atatggtaga ctatttttac
     2341 atgacttcaa atgaatagga cacaaataaa ggagggcaga tttatgcctc ttacattaaa
     2401 tgaattatat acacaaacca aacatcaata ccatctttcc atgatcgcag gtgaaactgg
     2461 gatcgatcat atcatgaact gggtttatgt ctctgaagac agttctaccc acgactttct
     2521 aaagggtgga gaactgatca tcacgacagg gatcaactgt caggatgagg cttctcttta
     2581 tgattttatt gaaacgatga tcaaaagcca tacttgcggg atgatcttaa atacaggaaa
     2641 atatattctt tccgaagata tcactgattc tattaaagca ttgtgcgaca aacacaacta
     2701 tccattgatc gagatgcctt ggaaagttca tatttatgat attacgagag attattataa
     2761 taagatcttt atggatacac agaaagatac ttcgatcacg gatgcatttt tatcttttat
     2821 tgaagaagat tccctgcacc attatgatgc tttgcgaatt cttgaagaca atcattttga
     2881 gaaagaagat tgttatgaga ttgctttaat gaaatgtaat aagaaagcga tctctgatga
     2941 tcttaaatta cgtctgctgt ttttgatgga aagttatatc aaactaaacg atctggatat
     3001 ccatattgct ttttataaga atcatttttt gtttgttttt catgacatgg aagaagaatt
     3061 gatttataac gaaatatctt ctttgatctc tacactgaaa agttcgttta aaaaaatgca
     3121 gatttatgca gggattggaa gttctgtcca cacgcttcgt gagattcgtg taagttatca
     3181 acgttctctg gctgctcttg tttatgctgc gaataatgga caagaaattg cccggtttga
     3241 ggagatggga ttctttcgaa ttcttcattc tgtgaatgat aaaaatcttt tgatggctta
     3301 tcatgatgag tatttaaaga atattgaaga gtatgatgag attcatgata caaattatct
     3361 ggagacgttg catcagtatc tgctttgtaa tgggagtatt cagcatgtgg cttcgaatat
     3421 gttttgtcat cgaaatacga ttacttatcg aatgcgtgtg atcgaggatc attggaattt
     3481 gaagttggac gatacggtgg agagatttca tttgatgaca gcgtttttta tccgggatta
     3541 tttgaatttg tagttatcaa tcatctaaaa gatgt
//
LOCUS       DAOMUE010000211         3544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7477_length_3544_cov_2.468902, whole genome shotgun sequence.
ACCESSION   DAOMUE010000211 DAOMUE010000000
VERSION     DAOMUE010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7477_length_3544_cov_2.468902"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLC71_08670"
     CDS             <1..654
                     /locus_tag="PLC71_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM02751.1"
                     /translation="AGEFVAVIGPSGSGKSTFVSLIARLYDVTSGRILYRGVDEKELK
                     PEFIRGKIGFVPQKASLFEGSLRDNMKWGKEDATDEEIYQALDIAQAREFVDQKEQGL
                     DFRIEQNGGNLSGGQKQRLTIARALVRKPEILILDDSASALDFATDARLRKAIKENTD
                     NMTVFLVSQRVSTIRNADHILVLDDGKIAGIGTHLQLLKENQVYKEICESQMAGEEA"
     gene            659..2407
                     /locus_tag="PLC71_08675"
     CDS             659..2407
                     /locus_tag="PLC71_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM02752.1"
                     /translation="MKKDKKQKTLQRIMEYMKPYRFLIFASLVLAVISVALTLYVPIL
                     TGQGVDCIISKGNVDFARLISIIKTIVICIVLTAAAQWLMNHVNNQITYKIGKDLRIQ
                     AFEHLQKLPLSYVDAHSSGDLISRIVTDIDQFTDGLLLGFTQLFTGVITIIGTICFML
                     GINPWITLVVVILSPFSFFIASFISKRSFNMFRKQSQTRGNMTGFVNEMLGNIKVVKV
                     FDHGEKAQEEFDQINDDLAYYSLRATFFSSITNPATRFMYSGIYAGVAIAGCMSVIRG
                     SISVGQLSSFLSYTNQYTKPFNEITGVITEFQNSLASAARVFELLDEEPMIPDAKDAK
                     ELQDVKGNVELEHVDFSYVKEVPLIQDFNLKVEPGQRIAIVGPTGCGKTTLINLLMRF
                     YDVNVGMIKVEGNDIRDVTRESLRSSYGMVLQETWLKAGTIRENICYGKPDATEEEMI
                     MAAKEANAHGFIERMPDGYDTIITEGGGNLSQGQKQLLCIARIMLSLPPMLILDEATS
                     SIDTMTEIRIQKAFETMMKGRTSFIVAHRLSTIQNADVILVMENGHILEQGTHEELLA
                     KNGAYARLYNSQFAPV"
     gene            2437..3015
                     /locus_tag="PLC71_08680"
     CDS             2437..3015
                     /locus_tag="PLC71_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRM02753.1"
                     /translation="MEEKGMKKKKKLLRLFIETFYLSAFTFGGGYMIVSLMKKKFVNE
                     YHWIEEDEMLDLVAIAQSAPGAIAVNGAIVVGYKLAGISGVIVSVFGAVLPPFLIISV
                     VSVFYEIFRDNRIVAAMLSGMQTGVGAVIISVVYEMAKGQSKKDNRIELAIIMVVAFM
                     ASYVFDISVIYIILGVGIVQYGISRKKTGGRE"
     gene            3012..>3544
                     /locus_tag="PLC71_08685"
     CDS             3012..>3544
                     /locus_tag="PLC71_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRM02754.1"
                     /translation="MIYLQLFLSFLKIGAFSFGGGYAAMPMIQQQVVDAYHWLSVSEF
                     GDLVTISQMTPGPIAINAATFVGIRVVGWQGALCATMGCVFPACVIVTVIAYFYVKYR
                     HMEGLQMVLNTLRPAVIALILTSGITILTQAFFQESHIDLQQINWIQGGIFIICMYLL
                     LRKKKDPIIVMLLAGVL"
BASE COUNT         1127 a          530 c          864 g         1023 t
ORIGIN      
        1 gccggtgaat ttgttgcagt cattggcccg agtgggtcag gaaagagtac ttttgtatct
       61 ttgatcgcaa gattatatga tgtgacatca ggaagaattc tataccgtgg agttgatgag
      121 aaagaattaa aaccagaatt tatccgagga aagatcggat ttgttccgca gaaagcatct
      181 ttgtttgaag gaagcctaag agataatatg aaatggggta aagaagatgc cacagatgaa
      241 gaaatctatc aggcattgga tatcgcacag gcaagagaat ttgttgatca gaaggaacag
      301 ggattagact ttagaattga acaaaatgga ggaaacttat ctggcggaca aaaacagaga
      361 ctaacgatcg caagagcatt agtacgtaaa cctgagatct taattcttga cgatagtgca
      421 tccgcacttg actttgcaac agatgcaaga cttcgaaaag cgatcaaaga aaatacagat
      481 aatatgacag tatttcttgt atcccagaga gtatcaacga ttcgtaatgc tgaccatatc
      541 ttagtgttgg atgatggaaa gattgcagga atcgggactc atctacagct attaaaagag
      601 aatcaggtat ataaagaaat ctgcgaatca cagatggcag gagaggaggc ataagatcat
      661 gaagaaagac aaaaaacaaa agacattaca aaggatcatg gaatatatga agccttatcg
      721 atttttgatt tttgcgtctt tagtgttggc ggtgatcagt gtggcactga cactttatgt
      781 tccgattctg acaggacaag gtgtggattg cattatatca aaaggaaatg ttgattttgc
      841 aagattgata agtattataa aaacgatcgt gatctgtatc gtattgacgg cggcagcgca
      901 gtggctgatg aatcatgtca ataaccagat tacatataag attggaaaag atttaaggat
      961 tcaggcattt gagcatttgc agaaactgcc attatcttat gtagatgcac actcctcagg
     1021 agacttgatc agccggatcg taacggatat tgatcagttt acggatggat tgctgcttgg
     1081 atttacacag ttatttactg gtgtgatcac gatcattgga accatttgtt ttatgttagg
     1141 aatcaatcca tggatcacat tggtcgttgt tattttaagt ccgttttcgt tttttatcgc
     1201 aagttttatc tcaaagcgaa gttttaatat gttccggaaa caatcacaga ctagaggaaa
     1261 tatgacagga tttgtcaatg aaatgcttgg aaatattaaa gtagtcaaag tgtttgatca
     1321 tggagaaaaa gcacaggaag agtttgatca gatcaatgat gatcttgcat attactcctt
     1381 aagagcgaca tttttctcat ctatcacaaa tccggcaacc agatttatgt attcaggaat
     1441 ctatgcaggg gttgcgatcg caggatgtat gagtgttatt cgcggaagta tcagtgttgg
     1501 gcagttatca agttttttaa gttatacaaa ccagtataca aagccattta atgagatcac
     1561 aggggttatc acagaatttc agaattcttt agcttctgcg gcaagagttt ttgaattatt
     1621 agatgaggag ccgatgattc ctgatgccaa agatgcgaag gagttacagg atgtgaaagg
     1681 aaatgtagaa ttagagcatg tggacttttc ttatgtgaaa gaagtacctt tgatccagga
     1741 ttttaatctg aaagtggaac caggacagcg gattgcaatc gttggaccga caggatgtgg
     1801 gaaaacaacg ttgatcaatc tgttaatgcg tttttatgat gtgaatgtag gaatgatcaa
     1861 ggtggaagga aatgatatcc gggatgtcac gagagaaagt ttacgatcca gttatggaat
     1921 ggtacttcag gagacatggt taaaagcagg aacgattcgt gaaaatatct gctatggaaa
     1981 accagatgca actgaggaag agatgatcat ggcagcaaaa gaagccaatg cacatggatt
     2041 tatcgagcga atgccagatg gttatgatac gatcattaca gaaggtggcg gaaatctctc
     2101 tcaaggacag aaacagctcc tttgtattgc aaggatcatg ttaagccttc ctccaatgct
     2161 gatcttagat gaagcgacat catcaattga tacgatgaca gagatccgaa ttcagaaagc
     2221 atttgaaacg atgatgaaag gaagaacaag ttttatcgta gcccaccgac tttcaacgat
     2281 tcagaatgcg gatgtgatac ttgttatgga aaatggacat attttggagc agggaacaca
     2341 tgaagaactt ttagcgaaaa atggtgcgta tgccagatta tataacagcc agtttgcacc
     2401 agtataaatg gtaagatttt tgactggaga tgtcatatgg aagaaaaagg aatgaaaaaa
     2461 aagaaaaaat tattgaggtt gtttatagaa acattttatt taagtgcatt tacctttggt
     2521 ggcggctata tgatcgtatc tttgatgaag aagaagtttg tgaatgaata tcattggatc
     2581 gaagaagacg agatgcttga tctggtggcg atcgcacaat cagccccagg agcaatcgca
     2641 gtgaatggag caatcgttgt tggctataaa ttagcaggta tcagtggtgt gatcgtaagt
     2701 gtattcggag ctgttctacc gccatttttg atcatatctg tagtctctgt tttttatgaa
     2761 atatttcggg ataacaggat tgttgcagcg atgctttctg gtatgcagac gggtgttggt
     2821 gcggtgatca tcagtgttgt gtacgagatg gcaaaagggc aaagtaagaa ggacaacaga
     2881 atagaacttg cgatcattat ggttgtggca tttatggcaa gttatgtctt tgatattagt
     2941 gtgatctaca ttattcttgg agttggaatc gtacaatatg gcatttcaag aaagaagaca
     3001 ggaggcagag aatgatctat ttgcaattat ttttaagctt cttaaagatc ggagcattta
     3061 gtttcggagg cggatatgca gcaatgccaa tgatacagca gcaggtggta gatgcatatc
     3121 attggctgtc tgtgtctgaa tttggagatc tcgtgacgat ttcacagatg acaccgggac
     3181 caatcgcaat taatgcggcg acttttgttg gtataagggt agttggatgg caaggagcat
     3241 tatgtgcaac gatgggatgc gtattcccgg cgtgtgtgat cgttacagtg attgcttatt
     3301 tttatgtaaa atatcgtcat atggaaggac tacaaatggt gttaaatacc ttacgtcctg
     3361 cggtgatcgc actgatctta acttctggaa ttacgatcct tacgcaggca ttttttcagg
     3421 aaagtcatat agatcttcaa cagatcaact ggatacaggg tggtattttt atcatttgta
     3481 tgtacctttt attgagaaag aaaaaagacc cgatcattgt tatgctgctt gcgggtgtgc
     3541 taaa
//
LOCUS       DAOMUE010000212         3540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7491_length_3540_cov_3.044763, whole genome shotgun sequence.
ACCESSION   DAOMUE010000212 DAOMUE010000000
VERSION     DAOMUE010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7491_length_3540_cov_3.044763"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PLC71_08690"
     CDS             <1..312
                     /locus_tag="PLC71_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02755.1"
                     /translation="MERALEEMIKISQDERERYLYLREEMAESDRASQIQSAQRIGRK
                     EGEILKLISLVQKKILKNKTLPQIAEESEEDTIVIEPIYTLIKENPEAEKEEIYKMLN
                     L"
     gene            498..1253
                     /locus_tag="PLC71_08695"
     CDS             498..1253
                     /locus_tag="PLC71_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRM02756.1"
                     /translation="MLNQFSRTELLLGKEALDKLAGSRVAVFGIGGVGGYVCEALVRS
                     GVGEFDLVDDDKVCLTNLNRQIIATRKTIGKYKVEVMRDRILEINPDAKVNVHKCFFL
                     PENADEFPFEEYDYIVDAVDTVTAKISLVMAAKEKNVPIISSMGAGNKLDPSQFKVAD
                     IYKTKVCPLAKVMRRELKKRRVKKLKVVYSEEMPIKPKDDMANNCRTNCICPPGAQHK
                     CTEKRAIPGSTAFVPSVAGLIIAGEVVKDLCKA"
     gene            1241..1945
                     /locus_tag="PLC71_08700"
     CDS             1241..1945
                     /locus_tag="PLC71_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRM02757.1"
                     /translation="MQGVKSVTKQAFMKSIPIMCSYIFVSMAYGMMMENAGFAWYYSL
                     LTSLTVYTGAFQFVLITFLSSGASIVTIAVTALLMNSRQSFYSLSFLETFRKMGRKKL
                     YMIHTMTDETYAVNCTIEDKDENSRKEMFLVAFFSRCYWMFGAVMGGLIGQLIPFELT
                     GIDFCMTALFIIIFIDQWEKADKHFPAVAGILIAVIALMIFGQRAFMLPALVIVSGVL
                     IFWNSKQQEIYTERAK"
     gene            1945..2271
                     /locus_tag="PLC71_08705"
     CDS             1945..2271
                     /locus_tag="PLC71_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRM02758.1"
                     /translation="MNTKMALIAIFVSAVITFGLRALPFLVFQEGKELPDQIKRLGMV
                     LPSSIMAVLIVYCLKDVGDDFMADGLWKLIAAAITAVSYRWKHNTLISILLGTVSYML
                     FLHFFG"
     gene            2319..3476
                     /locus_tag="PLC71_08710"
     CDS             2319..3476
                     /locus_tag="PLC71_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HRM02759.1"
                     /translation="MAREIETKCIHLEEEENSINHYGAISYPIYQSATYAHPGVGQST
                     GYDYSRLQNPTREHLEKTVASLENGIDALAFSSGMAAITLMMELFKPGDHLIVDADLY
                     GGSIRLFNHVSEKNGIEFSSVDCHKENVAAYIRENTKAVYIETPTNPMMNVTDIKALA
                     EITKKHNILLIVDNTFLSPYFQNPLDLGADIVVHSGTKYLGGHNDTLAGFLVTNREDI
                     SERFRFLIKTTGAGLAPFDCFLIQRGIKTLGVRMDRAQENAIEIAKWLKEQDIVKKVV
                     YPGFKEHPGYEIMKKQARGFGAMLTFELTKKEYAINILEKVRMIKYAESLGGVETLIT
                     YPMTQTHADVPEHIRKQNGITDCVLRMSVGIENVKDLLNELEEVFAEVRGE"
BASE COUNT         1255 a          582 c          805 g          898 t
ORIGIN      
        1 atggaaaggg cgctggaaga gatgataaag atcagtcagg atgagaggga aagatatttg
       61 tatctgagag aagagatggc agagagtgac agagcaagtc agatacaaag tgcacagagg
      121 attggacgta aggaaggaga aatattaaaa ttaatttcat tagttcaaaa gaaaattttg
      181 aaaaataaaa ctttgccaca gattgcagaa gaatcagaag aagatacgat agtaattgaa
      241 cctatttata cattaataaa agagaatcca gaggcagaga aagaagaaat ctacaaaatg
      301 ttgaatttgt aaggagttta acaatcgttt gttgacacat aaaaataaca acaccagtct
      361 tgacatccaa tcatcgtttg catataataa aaccaataga aatagtagga aatacaaaaa
      421 taccagcgga agcgcagcat aacaagaata gaaatgctaa gcatccgctt ttttattcta
      481 aataaaagga gaaagaaatg ttaaatcagt tttcaagaac agaactatta cttggaaaag
      541 aagcacttga taaattagca ggcagcagag ttgctgtctt tggaatcggt ggtgttggtg
      601 gatatgtatg cgaagcctta gtacgaagcg gtgtcggtga attcgacctt gttgacgatg
      661 ataaagtctg cttaacaaac ctaaaccgcc agatcattgc aacaagaaaa acaatcggaa
      721 aatacaaagt agaagtgatg agagaccgta ttttagagat caacccagat gcgaaagtca
      781 acgttcacaa atgtttcttc ttaccagaga atgccgatga atttccattt gaggaatatg
      841 actacatcgt tgatgccgtt gacacagtga cagcaaagat ctcattagtt atggcagcaa
      901 aggaaaagaa tgtaccgatc atcagcagta tgggagctgg aaataaatta gatccaagcc
      961 agtttaaagt ggcagatatc tataagacaa aagtatgccc tcttgcaaag gttatgcgaa
     1021 gagaattaaa gaagagaaga gttaagaaat taaaagttgt atattcagaa gaaatgccga
     1081 tcaaaccaaa agatgatatg gcaaacaatt gcagaacaaa ctgtatctgc cctccaggag
     1141 cacagcacaa atgtacagag aaaagagcga ttccaggcag tacagcattt gtaccatctg
     1201 tcgcaggact gatcatcgca ggggaagtag tgaaggattt atgcaaggcg taaaaagtgt
     1261 aacaaaacaa gcatttatga aatccatacc aatcatgtgc agctatatat ttgtcagtat
     1321 ggcatatgga atgatgatgg agaatgcagg atttgcgtgg tattattcgc ttcttacaag
     1381 ccttaccgtg tatacaggag cttttcagtt tgtcctgatc acatttttaa gtagcggggc
     1441 atcaatcgtt acgatcgcag tgacggcatt gttgatgaac agcagacaga gcttctacag
     1501 cctttcattt ttggagacat tccgtaagat gggaagaaag aaactttata tgatccatac
     1561 gatgacagat gaaacatatg cagtaaactg cacgatcgaa gacaaagatg aaaacagcag
     1621 aaaagagatg tttctcgttg cattcttcag cagatgttac tggatgtttg gagcagtgat
     1681 gggcggtctg atcgggcagt tgatcccatt tgaattaaca ggaattgatt tttgtatgac
     1741 tgcattattt atcatcattt ttatcgatca atgggagaaa gcggacaagc attttccagc
     1801 ggttgcagga attctgatcg cagtgatcgc cttgatgatc tttggacagc gggcatttat
     1861 gctgccagca ttggtgatcg tttcaggagt tttaatcttc tggaatagca aacaacagga
     1921 aatttataca gaaagggcaa aataatgaac acaaaaatgg cattgattgc gattttcgtt
     1981 tctgccgtga tcacatttgg acttcgagcc ctcccttttc ttgtatttca ggaaggaaaa
     2041 gagcttccag atcagataaa aagattagga atggtgttgc catcttcgat catggcagta
     2101 ctgatcgtgt attgcctgaa agatgtcggg gatgacttta tggcagatgg tttatggaaa
     2161 ctgatcgcag cggcaattac cgcagtcagc tatagatgga aacataatac gctgatcagc
     2221 attttacttg gaacagtgag ttatatgctg ttcttacatt tttttggata gaactaattt
     2281 gagatagaat aaaacatagg atgaaacagg agatatatat ggcaagagaa atagagacaa
     2341 aatgtattca tttagaagag gaagaaaatt cgatcaacca ctatggtgcg atcagttacc
     2401 caatctatca gtcagcaaca tacgcacatc caggagtcgg acaaagcaca ggttatgatt
     2461 acagccgcct gcagaatcca acgagagaac atctggagaa gacagttgct tctctggaaa
     2521 atggaatcga tgcattggca ttttcctcag gaatggcagc gatcacgttg atgatggaat
     2581 tattcaaacc gggcgatcat ctgatcgtgg atgcagattt gtatggtggc agcatccgtc
     2641 tatttaatca cgtatctgag aaaaacggaa tcgaattttc cagtgtggac tgtcacaagg
     2701 aaaatgttgc agcatatatc agagaaaata caaaagcagt ttatatcgag acaccaacca
     2761 acccaatgat gaacgtcaca gatatcaaag cattagctga gatcacaaag aaacataaca
     2821 tccttctgat cgttgacaat acatttttat ctccatattt ccagaatcca ttagatctcg
     2881 gagcagatat tgttgtacac agcggaacaa aatatcttgg aggccacaac gatacactgg
     2941 ctggattctt agttacaaac agagaagata tcagtgaaag atttcgcttc ctgatcaaga
     3001 caacaggagc tggattagca ccatttgact gtttcctgat ccagagaggg atcaaaacct
     3061 taggagtccg catggaccgt gcacaggaaa atgcgatcga gatcgcaaaa tggttaaaag
     3121 aacaggatat cgtaaagaaa gttgtttatc caggattcaa agaacatccg ggatacgaga
     3181 tcatgaaaaa gcaggcaaga ggatttggtg caatgcttac atttgaactt acgaagaaag
     3241 aatatgcgat caatatcctt gaaaaagtaa gaatgatcaa atacgcagaa agtcttggtg
     3301 gggtggaaac actgatcaca tatccaatga cacagacaca tgcagatgtt ccagaacata
     3361 tccgcaaaca aaacgggatc acggattgtg tgttaagaat gtctgtcgga attgagaatg
     3421 tcaaagattt attaaatgaa ctggaagagg tatttgcaga agtacgaggt gaataaactt
     3481 ttgaaaagga gtgatttgaa aaatgatgca gaagattcaa aaatttggag gtgcgatgtt
//
LOCUS       DAOMUE010000213         3535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7507_length_3535_cov_2.485345, whole genome shotgun sequence.
ACCESSION   DAOMUE010000213 DAOMUE010000000
VERSION     DAOMUE010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7507_length_3535_cov_2.485345"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLC71_08715"
     CDS             complement(<1..714)
                     /locus_tag="PLC71_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRM02760.1"
                     /translation="MKYTKQNIQKKRQILSSEKIRRNSKISLFLFKYAFIISIALIVT
                     GLGLGVGFVRGILKTTPQITKDSVHSKGYITTIYDNKGSTIKTLSNHDSNRIYTPLSS
                     VPKNLQHAFIAIEDERYYSHNGIDLYGIIRATFLGIRNQSLSQGGSTITQQLIKNNVL
                     GIQPEKTTMERLERKIKEQSLALELEKIASKQYILEEYLNAINLGEGTLGVQTASQKY
                     FNKDVSELTLSECAVLASIT"
     gene            843..1142
                     /locus_tag="PLC71_08720"
     CDS             843..1142
                     /locus_tag="PLC71_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HRM02761.1"
                     /translation="MKKQLKGKQSFYDDKQRENVVSYYLMEDQEHTMYGVELEKYQEE
                     TNVIEWDAVPSISESMELVDRVIHNLIKYKVTPISLAESLDEIMTREEEDGRSKI"
     gene            1123..1779
                     /locus_tag="PLC71_08725"
     CDS             1123..1779
                     /locus_tag="PLC71_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRM02762.1"
                     /translation="MDVQKYKILYKEGEAEISEKKSRFIAHIAPAQTEEEAQAFIEKI
                     KKQYWDARHNCWAYSIGEKQPALRCSDDGEPSGTAGKPMLEVLTGQELHNVVAVVTRY
                     FGGTLLGTGGLIRAYTKSTQEGIKESMVIEKCLGVMLSLTCDYTTSGKIQYLTATEHI
                     PVLDTVYTDNVTFEMIVPVEEVGSVEKKFMEASMGKAVLKKGEETYYAEIDGKISYDL
                     "
     gene            1880..2767
                     /gene="metF"
                     /locus_tag="PLC71_08730"
     CDS             1880..2767
                     /gene="metF"
                     /locus_tag="PLC71_08730"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRM02763.1"
                     /translation="MKISEILKNKSTISFEVFPPKNKEGDISSIYSTIEGLAKLSPDF
                     ISVTYGAGGSTKGKTVEIASVIKHEYGIESVAHLSCISSTKEDIKYECERLKEAGIDN
                     ILALRGDYPADATNFDPENLEFHYASELNEFIGEHFKGQFCLSGACYPETHQEADGFK
                     KDLEALKKKVDAGAEYLVTQIFFDNDYYYRLVREARKIGITVPILAGIMPITNAKSLI
                     RTTKMCGCTIPYQLSTMIEAHYDNPEAMKEIGINYAAQQIIDLITNGVDGIHIYTMNR
                     AETAQRVFDSIPAVLKELN"
     gene            2769..3425
                     /locus_tag="PLC71_08735"
     CDS             2769..3425
                     /locus_tag="PLC71_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HRM02764.1"
                     /translation="MDFQVVRENALKYLGYQAPMFEGLTPGISSLLGECIEEIEQIAE
                     PKVIVKRFKLAKDPLRLEESGDEILGQQMEDMLEDCEECILIGCTLGIVMERKIKFYA
                     KVNMTKSTVMDAAASAYLEEYCDRYEETLGFDNRTYRACPGYGDFPLEFNKRIAILLD
                     IPRKIGVTITPNNLLIPQKSMLGLIGIGSDKRKKSCGNCVMKEDCPFRKRGQRCYRTD
                     "
BASE COUNT         1252 a          518 c          792 g          973 t
ORIGIN      
        1 agtaatactt gcaagtacag cacattcgga caatgtgagt tcagaaacat ctttattaaa
       61 atatttttgt gatgctgtct gaacaccaag tgttccttct ccaagattga tcgcatttag
      121 atattcttct aagatatatt gcttagaagc aatcttttct agttcaagag caagagattg
      181 ttctttgatc tttcgttcca gacgttccat tgtggttttt tccggctgaa ttccaagtac
      241 attgttcttg atcaactgtt gcgtgatagt acttccccct tggcttagag attggtttct
      301 gataccaagg aaggttgctc gaataattcc atacagatca attccattat ggctgtaata
      361 acgttcatct tcaatcgcaa taaaagcatg ctgaagattc tttggaacgg aagaaagagg
      421 agtgtagatc ctgttggaat catggtttga aagtgttttg atcgttgatc ctttgttatc
      481 ataaatagtt gtgatatatc ctttggaatg gacggaatct tttgtaatct gtggggttgt
      541 ttttaaaatc ccacgtacga aaccaacccc gaggccgagc ccagtaacga tcaaggcaat
      601 ggagatgata aaagcatatt taaaaagaaa gagagaaatc ttagaatttc tacgaatttt
      661 ttctgaagat aagatctgtc tctttttctg aatgttttgt ttggtatatt tcataaaaaa
      721 cctcctgtta cgtattatac caaataaaaa agttcaagta aaccgaccgt gtaactataa
      781 acgatacaat caacaatatg atgatgtaag gggcaaaagt ataaagtata aaggagaaga
      841 atatgaaaaa acaattaaag gggaagcagt cattttatga tgacaaacaa agggaaaatg
      901 tagtatccta ctatttgatg gaagatcagg aacacacgat gtatggagtc gaactggaaa
      961 aatatcagga agaaacgaat gtgatcgagt gggatgcagt tccaagtata tcagaatcta
     1021 tggaattagt agatcgtgtg atccataacc tgataaaata taaagtaaca ccgatctcac
     1081 tggcagagtc cttagatgag atcatgacca gggaggaaga agatggacgt tcaaaaatat
     1141 aaaatcttat ataaagaggg agaagcagag atttcagaaa agaaatcaag atttattgca
     1201 catattgctc cagcgcagac agaagaagaa gcacaggcat tcatagagaa gataaaaaaa
     1261 caatactggg atgcaaggca taactgctgg gcatacagta tcggagaaaa gcaaccagcc
     1321 ttgcgttgta gtgatgatgg ggaaccaagt ggaactgctg ggaagccaat gcttgaggtg
     1381 ctgacagggc aggaactaca taatgtggta gcagttgtaa caagatattt tggtggaaca
     1441 cttcttggaa ctggaggatt gatccgtgcg tacacaaaaa gtacgcaaga agggatcaag
     1501 gaaagtatgg tgatcgagaa atgtcttggg gtaatgctca gcctcacttg tgattacacg
     1561 acatctggaa agattcagta tttaacagca acagagcata tcccagtgct tgatacagtg
     1621 tatacggata atgtaacatt tgaaatgatc gtaccagtgg aagaagttgg aagtgttgag
     1681 aaaaagttta tggaagcatc tatggggaaa gcagttctta aaaaaggaga agaaacttat
     1741 tacgctgaaa tcgatggtaa gatcagttac gatctataga cgaagaatga tatgatttcg
     1801 ttcctttaca gtgataaaat atcatgttaa aatttatcat agttaattag tcagaaataa
     1861 tgaaaaggag caaaattcta tgaaaatatc agaaatttta aaaaataagt caacaatttc
     1921 gtttgaggta tttcctccaa agaataaaga aggagatatt tcttccattt atagtacgat
     1981 tgagggatta gcaaaattat ccccggattt tattagtgtt acatatggtg caggtggatc
     2041 tacaaaaggt aagacagtag aaatcgcatc agtcattaag catgaatatg gaattgaatc
     2101 tgtagcacat ttaagctgta tttcatctac aaaagaggat atcaaatacg aatgtgaacg
     2161 tctgaaagaa gcaggaatcg ataatatctt agcattaagg ggagattatc ctgcagatgc
     2221 aacaaacttt gatcctgaga atctggaatt tcactatgca agtgaattaa atgaatttat
     2281 cggagaacat tttaaaggac aattttgctt atcaggagca tgttatccag agacacatca
     2341 ggaagctgat gggtttaaga aagatttaga agcattaaag aagaaagtag atgcaggagc
     2401 agaatattta gtaacacaga ttttctttga caatgattat tattacagat tagtcagaga
     2461 agcaagaaag atcggtatca cagtaccaat tcttgcaggg atcatgccaa ttacgaatgc
     2521 gaaatctctg atccgtacaa caaagatgtg tggatgtacg atcccatatc agttatctac
     2581 aatgatcgag gcacattatg ataatccgga agctatgaaa gaaatcggaa tcaactacgc
     2641 agcacaacag atcattgatc tgatcacaaa tggagttgat gggattcata tttacacaat
     2701 gaatcgtgca gaaacagcgc agcgtgtatt tgacagtatt ccagctgtat tgaaagaatt
     2761 aaactaagat ggattttcag gttgtaagag aaaatgcctt gaaatatctt ggatatcagg
     2821 caccaatgtt tgaaggtttg actccaggga tttcaagtct tttgggtgaa tgtatagaag
     2881 agatcgaaca gatagcagag cccaaagtga tagtgaaaag atttaaactg gcgaaagatc
     2941 cattgagatt agaggaatca ggggatgaga ttttagggca gcagatggaa gatatgctgg
     3001 aagattgtga agaatgtata ttgatcggat gtactcttgg gattgttatg gagcgtaaaa
     3061 tcaagtttta tgcaaaggtt aacatgacaa aatctacggt tatggatgct gcggcaagtg
     3121 cttatctgga agaatattgt gacagatatg aagaaacact tgggtttgat aaccgaacat
     3181 atcgtgcatg tcccggatat ggagattttc cacttgaatt caacaaaaga attgctatat
     3241 tactagatat accaaggaag atcggagtca cgatcacgcc aaataatctt ttgatcccac
     3301 agaagtctat gttaggattg atcgggatcg gaagtgataa acgaaagaaa tcatgtggaa
     3361 attgtgtgat gaaagaagat tgcccgttta gaaagagagg acaaagatgt tacagaaccg
     3421 attaggaaaa gaacttttaa tatttgatgg agcgatggga acacagctcc agaatgcagg
     3481 attaagtgca ggggatatcc cagaagaatt aaatattgac cgcccggatt tatta
//
LOCUS       DAOMUE010000214         3533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7512_length_3533_cov_2.342438, whole genome shotgun sequence.
ACCESSION   DAOMUE010000214 DAOMUE010000000
VERSION     DAOMUE010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7512_length_3533_cov_2.342438"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLC71_08740"
     CDS             complement(<1..312)
                     /locus_tag="PLC71_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM02765.1"
                     /translation="MKIRQQRTIEKNICKKMILLSFCMILMVSMAGCGNKRKENLTIT
                     NVSYDPTREFYEKYNKDFIKYYKDKYGKKVEVIQSHGGSGSQARSVVEGSNGDVVTLA
                     LE"
     gene            complement(533..1414)
                     /locus_tag="PLC71_08745"
     CDS             complement(533..1414)
                     /locus_tag="PLC71_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02766.1"
                     /translation="MKGAKYILTAAVIAMSSVMMTGCFKPSKDAVVESKYYQSLKDER
                     DKLSVQLKEEKKKTNSLNKKIKAIHATSGDQKIADYKSRVKDSRIIKVDFATNTIKNQ
                     SFAVTNIPVCKYVKKIVTGCNRMIGITPTDVEKQYKQSYSYALIDEDNTTFEFKVYGD
                     SYIVFDEIPENVYAYNGASTVGDALIDAKEQKNYSNVAARIADAQIVVTDKKMKFNDT
                     AIKVSKIIEKAKKLSGKDATLDTASWNEYRFYTSGTLTKILLGDRTVIGIEDKNGKQT
                     FYQISDKQKKNLKKYMK"
     gene            complement(1438..2106)
                     /locus_tag="PLC71_08750"
     CDS             complement(1438..2106)
                     /locus_tag="PLC71_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HRM02767.1"
                     /translation="MVELTNKEFFQDLDQTIAAQLFEQTTYPWEILPLIKDFVLEVGP
                     TLSVDEYDQVEEDVWIAKSATIAKTATINGPAIIGPDTEVRPGAFIRGNALIGAGCVV
                     GNSTEIKNDILFNNVQVPHYNYVGDSILGYKSHMGAGSITSNVKSDKTNVVIKNGEEK
                     IETGRKKIGAILSSRVEVGCGTILNPGSVVGHDTNIYPLSMVRGCIAPNSIYKNAGEI
                     AQKR"
     gene            complement(2162..2914)
                     /gene="xth"
                     /locus_tag="PLC71_08755"
     CDS             complement(2162..2914)
                     /gene="xth"
                     /locus_tag="PLC71_08755"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRM02768.1"
                     /translation="MKFISWNVNGIRACVKKGFLDFFNETDADIFCIQESKMQEGQLD
                     LNLPGYHQYWNYAVKKGYSGTGIFTKEEPISVFYGLGIEEHDQEGRVITLEFKDYYFI
                     TVYTPNSQSELARLSYRMKWEEDFLAYLKKLEEKKPVIFCGDLNVAVEEIDLKNPKTN
                     RKNAGFTDEEREKMRTILSNGFIDTFRYLYPDQTGIYSWWSYRFNARKNNAGWRIDYF
                     IVSECLKERISDAKILTDVMGSDHCPIELDFK"
     gene            complement(2915..>3533)
                     /locus_tag="PLC71_08760"
     CDS             complement(2915..>3533)
                     /locus_tag="PLC71_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM02769.1"
                     /translation="NFYAVDHLVLSDASCLNKLSPFFVIVFSYLVLKEKITPTQCACV
                     IAAFVGSLFIVKPSFAGGMTPAAVIGFAGGMCAGFAYTCVRKLGLRGERGPMIVLFFS
                     TFSMLACVPYLIMDFHPISARQLFFLLLTGLAAAGGQFGVTAAYTYAPAREISVYDYT
                     QIMFSAIMGLLVFGQLPDMLSFVGYCVIIAAGVIMFLYNKRRGGN"
BASE COUNT          977 a          797 c          521 g         1238 t
ORIGIN      
        1 ttctaaggcc agtgttacaa catcaccatt gcttccttct actactgatc ttgcctgact
       61 tccagaacca ccgtgggact ggatcacttc gactttcttt ccatatttgt ctttataata
      121 tttgataaaa tctttgttgt atttctcata aaactctctt gtcggatcat aagagacatt
      181 tgtgatcgta agattttctt ttctcttatt tccacatcct gccatagaaa ccataagaat
      241 catacagaaa cttaaaagga tcattttctt acagatattc ttttctatag ttctttgttg
      301 tcttattttc atggtttctt ctcctttatt ttaattccat actatttctg tatgttttgt
      361 atgaattgga ttatataaga tttctgtata catgtcaaca aatatcgtta taaaaattta
      421 taataactga ttcaaagaaa tttttattat gttcatcttt gctcataaaa gcacgaaaaa
      481 aagacgtttt gacatatgtt ttcaatacgt caaaacgtct ttctttcatt tattatttca
      541 tatatttttt cagattcttc ttttgtttgt cactgatctg ataaaaagtc tgttttccat
      601 tcttatcttc gattccaatg acagtacgat ctccgagaag tatttttgtt aatgtaccac
      661 ttgtatagaa acgatactca ttccatgagg ctgtatccag tgtggcatct tttccagata
      721 acttcttcgc tttctcaata atcttagata ctttgatcgc tgtatcatta aatttcatct
      781 tcttatctgt tacaacgatc tgggcatctg caattcttgc tgctacgtta ctgtaattct
      841 tctgctcttt ggcatcgatc aacgcatctc caacggttga agcaccatta tatgcgtaaa
      901 cattctctgg aatctcatca aacacaatat aactgtcacc atatactttg aattcaaacg
      961 ttgtattatc ttcatcaatc aacgcataag aataactttg tttatactgc ttctctacat
     1021 cggttggtgt gattccgatc attcgattac atccagttac aatcttcttt acatatttgc
     1081 agacaggaat attcgttact gcaaaactct gattcttgat cgtattcgtt gcaaagtcaa
     1141 ctttaatgat cctgctgtct ttgacacgac ttttgtagtc tgcaatcttt tgatctccag
     1201 aagttgcatg gattgctttg atcttcttat ttagtgaatt tgtttttttc ttttcttctt
     1261 ttaactgaac agacagttta tctctttcat ctttaagact ctgataatac tttgattcca
     1321 cgacagcatc ttttgacggc ttaaagcatc ccgtcatcat cacgctgctc attgcgatca
     1381 ccgctgctgt tagtatatat tttgcacctt tcatatggtt tccctccatt acaggtccta
     1441 tcgtttttga gcgatctctc ctgcgttctt atagatgctg tttggtgcga tacatccacg
     1501 taccatagat aatggataaa tattggtatc atgaccaacc actgatcctg gattcaagat
     1561 agttccgcat cctacttcta cacgagaact taagatcgca ccgatcttct ttcttccagt
     1621 ctcaatcttt tcttctccgt ttttgatcac aacgtttgtc ttgtcagatt ttacattcga
     1681 tgtgatagat ccagctccca tatgagactt atatcctaaa atagaatccc caacataatt
     1741 ataatgaggc acctgaacat tattaaataa aatatcattc ttgatctctg tagaatttcc
     1801 tacaacacat cctgccccga tcaatgcatt tcctctgata aatgctcctg ggcgcacttc
     1861 tgtatctgga ccaataatcg ctggtccatt gatcgttgcc gtcttggcga tcgttgcact
     1921 tttcgcgatc catacatctt cttctacctg atcatattca tctactgata gtgttggtcc
     1981 aacttccagt acaaaatctt tgatcaatgg aagaatctcc catggatacg ttgtctgttc
     2041 aaataattga gctgcaattg tctgatctaa atcctgaaag aattctttgt ttgttaattc
     2101 taccatctga ttttacccct ttctgttctt attacctgtt tcaatccaca gtctgtaatt
     2161 ttcatttgaa atccaactcg atcggacagt gatctgatcc catcacatct gttaagatct
     2221 tcgcatcact gatccgttct tttaaacact ctgatacaat gaaatagtcg atacgccacc
     2281 ctgcgttatt ctttctcgca ttgaagcggt aagaccacca ggaatagatt cctgtctggt
     2341 ctgggtataa ataacggaag gtatcgataa atccattgct aaggattgtt ctcatttttt
     2401 cacgttcttc atccgtgaaa ccagcatttt ttctatttgt ctttgggttc ttaaggtcga
     2461 tctcctcgac tgcgacattc agatctccac aaaagatcac cggctttttc tcttctaact
     2521 tcttcaaata agcaagaaaa tcctcttccc acttcattcg ataagaaagc cttgccagtt
     2581 cactttgtga attcggtgta tacactgtga taaagtaata atctttaaac tccagtgtga
     2641 tcacacgtcc ttcctgatca tgttcttcta tcccaagacc ataaaaaacg ctgatcggtt
     2701 cttcttttgt aaaaatgcct gttcctgaat atcccttctt cactgcataa ttccagtact
     2761 ggtgatatcc cggaagattc aggtcaagct ggccttcctg catcttactt tcctgaatgc
     2821 agaaaatatc ggcatctgtc tcattgaaaa aatccaaaaa tcctttcttc acacatgctc
     2881 tgatcccatt gacattccat gatatgaatt tcatctaatt tcctccacgt ctcttattat
     2941 aaagaaacat aatcactcct gctgcaatga tcacacaata tcctacaaaa ctcagcatat
     3001 ctggaagctg tccaaaaacg agaagcccca taattgctga gaacatgatc tgtgtgtaat
     3061 catatacaga aatttccctt gctggtgcat acgtatatgc cgctgtgact ccaaactgtc
     3121 ctcctgcagc agctaatcct gttaataaca aaaagaataa ctgccttgct gaaatcggat
     3181 gaaaatccat gatcaaatat ggtacacacg ccaacataga aaatgttgag aagaaaagca
     3241 cgatcattgg tcctctttct cctctaagtc caagttttct cacacaggta tatgcaaaac
     3301 ctgcacacat tccccctgca aagccgatca cagctgctgg tgtcatcccg cctgcgaaac
     3361 ttggttttac aataaataaa ctaccgacaa aagctgcgat cacacatgcg cattgtgtcg
     3421 gtgtgatctt ctctttcagt actaaataag aaaatacaat aacaaaaaat ggcgataact
     3481 tatttagaca tgaagcatct gaaagcacca ggtgatctac cgcataaaag ttg
//
LOCUS       DAOMUE010000215         3511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7571_length_3511_cov_2.380208, whole genome shotgun sequence.
ACCESSION   DAOMUE010000215 DAOMUE010000000
VERSION     DAOMUE010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7571_length_3511_cov_2.380208"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>383)
                     /locus_tag="PLC71_08765"
     CDS             complement(<1..>383)
                     /locus_tag="PLC71_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRM02770.1"
                     /translation="DVLYEDENVIMVNKPWGILSQKSKPEDISINDQIIPYAVNQGLI
                     SEKELQVVKPSICNRLDRNTTGIIICGFSIEGLQTMAEMLKHRDMENFVVTHVVFRLD
                     KEFEPILTYGGLASHFPDRHALTAD"
     assembly_gap    384..393
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<394..632)
                     /locus_tag="PLC71_08770"
     CDS             complement(<394..632)
                     /locus_tag="PLC71_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRM02771.1"
                     /translation="MRELNITTREEGQRLDKILSKYLNKAPKSFVYKMLRKKNIKLNG
                     KKATGNEKLSQGDQVCIYLAEDTINKFREEVKAGK"
     gene            complement(642..1403)
                     /locus_tag="PLC71_08775"
     CDS             complement(642..1403)
                     /locus_tag="PLC71_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRM02772.1"
                     /translation="MRNIALVTGASSGMGKEFVREISKKYKGLDEIWVVARRRERLRE
                     LKKEIIGTRVRILPLDLMEEESFERLKMVLAKEQPVVRILVNASGYGISGSFEHQTEE
                     DAAGMIHLNCEALTRITYLVLPYLRRGSFIYQMASSAGFAPQPNFTVYAATKAYVKSF
                     SIALRQELSYQGIRVIAVCPGPVKTEFFDRAYEQEEMKFYKKFVMADPKKVVRKAIRD
                     ARKNKAVSVYGMTMKVTRVICKLLPGRWIAKIMGK"
     gene            complement(1430..2101)
                     /locus_tag="PLC71_08780"
     CDS             complement(1430..2101)
                     /locus_tag="PLC71_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRM02773.1"
                     /translation="MLENKKAVIFDLDGTLVDSMWIWREIDIRFLGKYGLEVPQGLND
                     KLEGYSFHETAVYFKEHFPLPLTIEEIMGTWNRMASEIYINEIRLKEGVKEFIELLKK
                     RNMKLGIATSNSRKLAKDCLRSNGILDAFDYICTSDEVPRSKPEPDVYLHAAKMIDTR
                     PKDALVFEDIPYGILAGKRAGMEVCAVKDPYSQGSVKEKKEIADYYINTYYDILDGTY
                     EVLKK"
     gene            complement(2102..2839)
                     /locus_tag="PLC71_08785"
     CDS             complement(2102..2839)
                     /locus_tag="PLC71_08785"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRM02774.1"
                     /translation="MVIMAKKTRLDKFLCDSLNMTRKEAKESVKKGKVVVNGEAIKKP
                     EYKVDAEQDQVEFDHKLIQFEQFHYLMLHKPQGVVSATKDHHDQTVLDLIHEDYKDRL
                     FPVGRLDKDTEGLLLLTDDGMLAHELLSPRKHVDKVYFAKVSGQFGENEIARFKEGLD
                     IGNGERSKQARLQILKADVQESEVLITIMEGKFHQVKRMVKAVGSEVLYLKRLSMGSL
                     KLDEELKLGEYRRLTKAELQELKEHRR"
     gene            complement(2830..>3511)
                     /locus_tag="PLC71_08790"
     CDS             complement(2830..>3511)
                     /locus_tag="PLC71_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HRM02775.1"
                     /translation="YSTCTFSPEENEQVIEYLLEKNEDLSLVPMKMCEGFDHGHPEWT
                     LTGREDIKQCIRLWPHKIKGEGHFLALLKKADGEQPTFKYEKIKKVKLTPETEEFFKN
                     CKMDIDWSHVREHQGKLFYLKEDIPEMKKVRVLRKGLYLGEMKKGRFEPSQSFAVALA
                     ANEYEPYLSFDINDENTVKYLKCETLDVDTNTEGIHLVGTNEFPLGWAKIKKGRLKNK
                     YSSSWRWL"
BASE COUNT          893 a          828 c          502 g         1278 t
ORIGIN      
        1 catcggctgt aagcgcatgt ctgtccggga aatgcgatgc aaggccaccg tatgtcagaa
       61 tcggctcgaa ttctttgtcg agacggaata ctacgtgagt gaccacaaag ttttccatat
      121 cacgatgttt tagcatttct gccatcgtct gtaatccttc tatagaaaac ccacaaatga
      181 tgattcctgt tgtattgcgg tccaatcgat tacaaatcga tggttttact acctgcaatt
      241 ccttttcact gatcaaccct tggttcactg catatgggat gatctggtca ttgatggaaa
      301 tatcctctgg ttttgatttc tgtgacaaaa ttccccatgg tttattgacc ataatgacat
      361 tctcatcttc atataaaaca tcannnnnnn nnntgctttc ctgctttaac ttcttcacgg
      421 aacttgttga tcgtatcttc tgcaagataa atacacacct gatctccttg agataatttc
      481 tcatttcccg ttgctttttt gccgtttaac ttgatatttt tctttcgaag catcttgtat
      541 acaaaactct ttggagcttt atttaaatat ttcgaaagaa tcttatctaa tctctgtcct
      601 tcttctcttg tcgttatgtt taattctctc attttatatc cttatttacc catgattttt
      661 gcgatccatc ttccaggaag aagcttgcag ataaccctag ttactttcat cgtcattcca
      721 taaacagata ctgctttatt ttttctcgca tcacggattg cttttcttac aactttcttt
      781 ggatctgcca ttacgaattt cttataaaat ttcatctctt cctgttcata tgcacggtca
      841 aaaaactccg tttttactgg ccctggacat actgcgatca ccctgatccc ttgataggac
      901 agctcctgcc gcaatgcaat agagaaactt ttcacatatg ctttggttgc tgcataaact
      961 gtgaaattcg gctgcggagc aaatcccgca gaagatgcca tctgataaat aaaagatcct
     1021 cttcttaaat atggcaatac aagatatgta attcttgtta atgcttcaca gtttaaatgg
     1081 atcattcctg ccgcatcttc ctctgtctga tgttcaaaag accccgaaat cccatatcca
     1141 gaagcattca ctaaaattct taccactggc tgttcttttg caagaaccat tttaagacgt
     1201 tcaaagcttt cttcctccat aagatctaat ggaagaatcc tcactcttgt tccaatgatt
     1261 tcttttttta gttctcttag ccgctctctt cgtctggcaa cgacccagat ctcatcaagc
     1321 cccttgtatt tcttcgagat ctcacggaca aattcttttc ccattccgga ggatgctccg
     1381 gttaccaatg cgatatttct catatgatca tcctttctcc tggcatctgt tatttcttta
     1441 atacctcata ggttccatct agtatgtcat aatatgtatt aatataataa tctgcaattt
     1501 ctttcttttc tttcactgat ccttgagaat atggatcttt cactgcacac acttccattc
     1561 ctgcacgttt ccctgccaaa atcccatatg gaatatcttc aaagaccaat gcatcctttg
     1621 gtctggtatc gatcatcttt gctgcatgca aataaacatc cggttccggc ttacttcttg
     1681 gaacttcatc ggacgtgcag atataatcaa atgcatccaa aatcccattg gatcttaaac
     1741 aatctttcgc cagctttctt gaattgcttg ttgcgattcc aagtttcata ttccgttttt
     1801 ttaacaactc aataaattct ttcacgccct cttttaaacg aatttcattg atatagattt
     1861 cggaagccat acgattccat gttcccatga tctcttcgat cgtaagcggc aacggaaaat
     1921 gttccttaaa ataaactgct gtttcatgaa aactatatcc ctctaattta tcatttaacc
     1981 cttgcggaac ttccaatcca tattttccca aaaagcgaat atcaatctct ctccagatcc
     2041 acatggaatc aactaatgtc ccatcaagat caaagattac tgctttttta ttctctaaca
     2101 tttatctcct atgctccttt agttcttgta actctgcctt cgttaatctt cggtattctc
     2161 caagctttaa ctcttcatcc aattttaatg atcccattga cagccttttt aaatataaca
     2221 cttcacttcc aacagctttt accatccgtt ttacctgatg aaatttccct tccatgatcg
     2281 tgatcagaac ctctgattct tgtacatctg ctttaaggat ctgaagtctt gcttgttttg
     2341 atctttcgcc attgccaata tccagacctt ctttgaatct ggcgatctca ttttcgccaa
     2401 actgccctga aacttttgca aaatatactt tatccacatg ttttcttggc gaaagaagtt
     2461 catgtgccag cattccatca tccgttaata ataataatcc ttctgtatct ttatccaatc
     2521 ttcctacggg gaacaatctg tctttataat cttcatggat cagatcgagt acagtctgat
     2581 catgatgatc cttcgttgca gatacaaccc cttgtggttt atgtaacatc agataatgga
     2641 actgttcaaa ctgaatcagt ttgtggtcaa attctacctg atcttgttct gcatccacct
     2701 tatattctgg tttcttgatc gcttctccat taaccacaac tttacccttt ttgacagact
     2761 ctttcgcttc ttttcttgtc atatttaaac tgtcacacaa aaatttgtct aatctggttt
     2821 tctttgccat tataaccatc tccagctgga agaatactta ttcttcagtc gtcctttttt
     2881 aatcttcgcc catcctaatg gaaattcatt tgttccaacc agatgaattc cttcggtatt
     2941 tgtatctacg tctaatgtct cacattttaa atatttgacc gtattctcat cgttgatgtc
     3001 aaacgaaaga tatggttcat actcattggc cgccaacgct actgcaaatg actgactcgg
     3061 ttcgaatctt ccttttttca tctctcctag ataaagacct ttccttagaa ctcttacctt
     3121 cttcatctct ggaatatctt cttttaggta aaacaatttt ccttgatgtt ctctaacatg
     3181 actccagtca atatccatct tacagttttt gaaaaattct tctgtctctg gtgtcagttt
     3241 tactttctta atcttttcgt atttgaatgt tggctgttct ccgtctgctt tcttcaataa
     3301 ggcaagaaaa tgtccttctc ctttgatctt atgcggccat aatcgaatgc attgtttgat
     3361 gtcttctctt cctgtcaaag tccattctgg gtgtccgtgg tcaaatcctt cacacatctt
     3421 cattggcaca agagaaagat cttcattctt ctctaaaaga tattcgatca cctgttcatt
     3481 ttcttcgggt gaaaatgtgc aggtagaata a
//
LOCUS       DAOMUE010000216         3470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7696_length_3470_cov_2.831918, whole genome shotgun sequence.
ACCESSION   DAOMUE010000216 DAOMUE010000000
VERSION     DAOMUE010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7696_length_3470_cov_2.831918"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="PLC71_08795"
     CDS             <1..979
                     /locus_tag="PLC71_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRM02776.1"
                     /translation="TKIIKDNLAEDGSRYGTQKVLVKMTTGTYKGKEKQAINPSGTLF
                     GADCKVGARIIVIVNKSGTNVGFTVYSQDRTMAIYGFALIFAAVVCAIGGKKGVYAIL
                     SLVFAFACIIGIMFPMMYRGISPIVLTIIVAVLTTIVTLGLLGGYSSKTVSAILGTAI
                     GVVIAAVAAMAFGKAAGITGYNVSDIEALNYVGQNTKIKIGELLFAGIIISALGAVMD
                     VGMSIASTIQEIYETDKTLSMKRLFVSGINVGRDMMGTMTNTLIFAYVGGAITTLVIN
                     YAYDLSYRQLANSYVIGIEIMQGLSGSLGVVLTVPITALIASFLAVRKK"
     gene            1352..>2267
                     /gene="aroF"
                     /locus_tag="PLC71_08800"
     CDS             1352..>2267
                     /gene="aroF"
                     /locus_tag="PLC71_08800"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRM02777.1"
                     /translation="MIIILKKQAEEAKINALKKEMEEQGLGIHESEGINTRILGLVGD
                     TSVVDMRHIMANSIVENVQRIQEPYKKANRKFHPNDTVVDVNGVKIGGGNFQVIAGPC
                     SIETKEQITEVANDVKNSGAGLLRGGAFKPRTSPYSFQGLHGEGLELLLEAKKATGLP
                     VVTEIMDASHLPLFEDVDLIQVGARNMQNFELLKELGKINKPILLKRGLAATIEEWLM
                     SAEYIMAGGNENVILCERGIRTYETAMRNTLDLSAIPMIKKKSHLPVMVDPSHATGIR
                     DMVEPMALAAIAAGADGLMVEVHNDPAKA"
     assembly_gap    2268..2277
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2482..3048
                     /gene="pth"
                     /locus_tag="PLC71_08805"
     CDS             2482..3048
                     /gene="pth"
                     /locus_tag="PLC71_08805"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRM02778.1"
                     /translation="MKVIVGLGNPGKKYDNTRHNIGFEALDYIADKEGISINTGKHKA
                     LIGTGYMGGEKVLLVKPQTFMNLSGESLRPIMDFYKLEPEDFIIIHDDIDLDVGRLRI
                     RRKGSAGGHNGLKSIISHLGSMDFPRVKIGVGEKPKGYDLADYVLGHFTKEEQAIFAE
                     RFDEVYDAVQLIVMGDITEAMNRHNKKK"
     gene            3058..>3470
                     /locus_tag="PLC71_08810"
     CDS             3058..>3470
                     /locus_tag="PLC71_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRM02779.1"
                     /translation="MLENILKDDPVYRQLKDEVEDYCGPVLVSGCTDTAKWHLTSLIG
                     NHKKKQFKIIIVPDENKARQWVEASHFFGEKVQYIPAKDPLFYTADVHGNAIARDRML
                     AIKKIVDDKCGTFVMTIDAVMDQVVPLSDIKNHKMT"
BASE COUNT         1224 a          556 c          798 g          882 t
ORIGIN      
        1 aacaaagatc attaaagata atcttgcaga agacggaagt cgttatggaa cgcagaaagt
       61 tctggttaaa atgacaacgg gaacctacaa aggaaaagaa aaacaggcga tcaatccaag
      121 cggaacttta tttggagctg actgtaaagt aggagcaagg atcatcgtga tcgtaaataa
      181 aagtggaaca aatgtaggat ttaccgtata tagtcaggac cgtaccatgg caatctatgg
      241 atttgcgttg atctttgcag cggtggtatg tgcgattggt gggaaaaaag gtgtttatgc
      301 tattttaagt cttgtatttg cgtttgcatg tatcattggt atcatgttcc cgatgatgta
      361 tcggggaatt tcaccgatcg tgttgacgat cattgtagca gtgttgacaa cgatcgtaac
      421 attaggactt cttggtggat attcgagcaa aacagtgtct gcgatccttg gaacagcgat
      481 cggagtcgtg atcgcagcag tggcagcgat ggcatttgga aaagcagcag ggatcacagg
      541 atataatgta tcggatatcg aagcattgaa ctatgtcgga caaaatacaa agataaagat
      601 cggagaattg ctttttgccg gaatcatcat ttcagcatta ggagccgtca tggacgttgg
      661 tatgtccatt gcatctacga ttcaggaaat ttatgaaaca gacaaaacac tgtccatgaa
      721 acgacttttt gtatcaggaa tcaatgttgg aagagatatg atgggaacga tgacgaatac
      781 actgatcttt gcttatgtgg gcggtgcaat aacaacatta gtgatcaatt acgcatatga
      841 tctaagctac agacagttag cgaactctta tgtgatcgga attgagatca tgcaaggatt
      901 atctggaagt cttggtgttg tattaacagt accgatcaca gcattgatcg catcatttct
      961 tgcagtgcgc aaaaaataaa ttttataatt cataaaataa atcataaata cggtctatgg
     1021 attttataaa aaacatctat aaatctattg acacagaaaa aaatttcatg tatcgtaaaa
     1081 acatcaaaac aaggagaaac taagatgaca acaagtaaga tgaataattg cacattttat
     1141 tactttagct ttcagggtta ttatttttat cctgactatt tttgttgcaa tgaatcatcg
     1201 ccaaaactta tgtcacttta aaccgacaga gtaaaacatt taaggcatgc attcttgcaa
     1261 cagagtgcat gccttttctt tttactcaaa gaaacaaagg ccgcaccaga gaagcaggat
     1321 agaaacgact tgccaggaaa ggacgaacat catgattatt attttaaaga aacaggcaga
     1381 agaagcaaag atcaacgcat taaagaaaga gatggaagag cagggactgg gaatccacga
     1441 atccgaaggt attaacacaa ggatccttgg attagtggga gatacatctg ttgtagatat
     1501 gcgtcatatc atggcaaaca gcatcgttga aaatgtacag cgtattcagg agccatacaa
     1561 gaaggccaac agaaaattcc atccaaacga tacagttgtt gatgtaaacg gagtaaagat
     1621 cggtggtgga aacttccagg tgatcgcagg accatgttct attgagacaa aagaacagat
     1681 cacagaagtc gcaaacgatg tgaagaattc tggtgcagga ttattaagag gaggagcttt
     1741 caaaccaaga acttccccat actctttcca gggacttcat ggagaaggat tagaattatt
     1801 attagaagct aagaaagcaa caggacttcc agttgttaca gagatcatgg atgcaagtca
     1861 tcttccatta ttcgaagatg tggacctgat tcaggttgga gcaagaaata tgcagaactt
     1921 tgaattatta aaagaattag gaaagatcaa caaaccaatc ttattaaagc gaggacttgc
     1981 agcaacgatc gaagaatggt taatgagtgc agaatacatt atggcaggtg gaaatgaaaa
     2041 tgtaatcctt tgtgaaagag gaatccgaac atacgagaca gctatgagaa atacattaga
     2101 tctttctgca atccctatga ttaagaagaa atcccatctt ccagtcatgg tagatccatc
     2161 tcatgcaaca ggaatcagag atatggtaga gccaatggca ttagcagcga tcgcagctgg
     2221 ggccgatgga ttaatggtag aagttcataa tgatccagcc aaagcatnnn nnnnnnngac
     2281 accagaacag tttgatacat tgatgaagaa ggtaaataag acaagagaat tctttcaggc
     2341 attataacta gaaaaaatct tgctgagaat tgtaaattgg gatatactat aactaaagaa
     2401 agaaaaagac aacccgggaa tccattgatt tccgggtgtt tgtcagtttt atagattcta
     2461 gggaaaaagg agagatatct tatgaaagta atcgtcggac tggggaatcc gggaaagaaa
     2521 tatgacaaca caagacacaa tattggattt gaagcccttg attatattgc agacaaagaa
     2581 ggcataagta taaatacagg gaaacacaaa gcattgatcg gaactggtta tatgggagga
     2641 gaaaaagtcc ttcttgtgaa accacagaca tttatgaact taagcgggga aagtcttcgt
     2701 ccaatcatgg atttctataa attagagcca gaagatttta tcattattca tgatgatatc
     2761 gaccttgatg ttggaagact gcgtatccga agaaagggaa gtgcaggggg acataatgga
     2821 ctgaaaagta tcatcagtca tttaggaagt atggatttcc caagggtgaa gatcggtgtt
     2881 ggagagaaac caaaaggata tgatctggca gattatgtat taggacattt tacaaaagaa
     2941 gaacaggcaa tctttgcgga acgttttgat gaagtgtacg atgcagtaca gctgatcgtt
     3001 atgggcgaca tcacagaagc gatgaaccgc cacaataaga aaaagtaggg atagaaagtg
     3061 ttagaaaata tattaaaaga cgatccggta tacagacaat taaaggatga ggtcgaagat
     3121 tactgtggcc cggttcttgt atcaggatgc accgatacag ccaaatggca tctgaccagt
     3181 ctgatcggaa atcataagaa aaagcaattt aagatcatca tcgtaccaga cgagaacaaa
     3241 gcaagacaat gggtcgaggc aagtcatttc tttggagaaa aggttcagta tattccagca
     3301 aaagatccat tattctacac agccgatgtt catggaaatg caatcgcaag agaccgtatg
     3361 ctggcgatca agaagatcgt ggatgacaaa tgtggaacct ttgtgatgac gatcgatgct
     3421 gtgatggatc aggttgttcc attaagtgat atcaagaatc ataagatgac
//
LOCUS       DAOMUE010000217         3445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7773_length_3445_cov_2.117994, whole genome shotgun sequence.
ACCESSION   DAOMUE010000217 DAOMUE010000000
VERSION     DAOMUE010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7773_length_3445_cov_2.117994"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLC71_08815"
     CDS             <1..682
                     /locus_tag="PLC71_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HRM02780.1"
                     /translation="KKQTDDWAKVKVDGQTGYVSKDYAKVTYSFGKAKSMKQIQAEQE
                     AKEAKKRAEEAARSKSENESVSTTSSGHTSDDGTTSGSTTGSTQTTKKASVSVSSSGS
                     SATGSRIASYAQQFVGNPYRYGGNSLTNGIDCSGFTQQIMAKFGYSISRTSSSQSGDG
                     RAVSTSNLRAGDLVFYGDGGSIDHVALYIGGGQVVHASNSAPYPRGGIKISNVNYRTP
                     ICARRIIG"
     gene            900..2237
                     /locus_tag="PLC71_08820"
     CDS             900..2237
                     /locus_tag="PLC71_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM02781.1"
                     /translation="MKQRRFTNKKLIALMLPLAVDQLLNSFMGTVDTLVVSNLGSAAI
                     SAVSLVDSINILVVQAFFALASGGTVVCSHYLGCEKKERATNAARQLLFITFAMSLVI
                     AIACHLFSNQLLGVIFGQVEPAVMDNAKKYFFFSAMSYPFIALYDDGACILRAQENSK
                     LPMQISFVANGINVALNLIFVWVLHLGVAGSSAATMIARAFAMVAVLYKLRNPKMKIQ
                     LRDYFSIRPEWEEIKRILHIGVPSGIENGMFQFGKLAIQSTVSLMGTAAIAAQGMTNI
                     IENLNGIMSIGMGIGLMTVVGECLGAGEKEEAVYYIKKISIAAEVVLIATCLLMFGLT
                     YPITYFGGMEPESAKLCIFMVTCITIVKPIVWIMAFIPPYGFRAAGDVRFTMTTSMLC
                     MWFCRVVLAMVLARVFHMGPIAVWIGMFTDWTIRGIIYTIRFKNRKWLAHQVI"
     gene            2322..>3152
                     /gene="queA"
                     /locus_tag="PLC71_08825"
     CDS             2322..>3152
                     /gene="queA"
                     /locus_tag="PLC71_08825"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRM02782.1"
                     /translation="MKVSDFTFDLPEELIAQDPLEDRSSSRLLTLDKNTGEIGHDVFH
                     NIVNYLKPGDCLVLNNTKVIPARLIGAKEETGGKVEVLLLKRKQDNVWETLVKPGKKA
                     RPGARISFGDGKLVGEVIDVVDEGNRLVKFEYEGIFEEVLDELGQMPLPPYITHQLKD
                     KNRYQTVYAKYDGSAAAPTAGLHFTKELLQQIKDMGVNIAYVTLHVGLGTFRPVKVDD
                     VLEHHMHSEFYRIEEKDAELINETKKNGGRVISVGTTSTRTLESVAEEDGTLHAKSGW
                     T"
     assembly_gap    3153..3162
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3163..3326
                     /locus_tag="PLC71_08830"
     CDS             <3163..3326
                     /locus_tag="PLC71_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRM02783.1"
                     /translation="IDGLITNFHLPESTLLMLVSALAGREHILNAYNIAVKERYRFFS
                     FGDAMFIHP"
BASE COUNT         1144 a          545 c          801 g          945 t
ORIGIN      
        1 aaagaaacag acggatgact gggcaaaagt taaggtcgat ggtcagacag gatatgtgtc
       61 aaaagattat gcaaaagtaa catactcttt tggaaaagcg aaatccatga agcagattca
      121 ggcagagcaa gaagcgaaag aggcgaagaa aagagcagaa gaagcagcca gaagtaagtc
      181 tgaaaatgaa tcagtatcca caacatcaag cggtcatact tctgatgatg gaacaacatc
      241 tggaagtaca acaggttcca cacagacaac aaagaaagct tccgtatccg tatcatctag
      301 tggctcatca gcaacaggaa gcaggatcgc aagttatgcg cagcagtttg ttggaaatcc
      361 ataccgatat ggtggaaaca gcttaaccaa cggaattgat tgttctggat ttacgcagca
      421 gattatggcg aagtttggat acagtatcag tcgtacgagc agttcccagt ctggtgatgg
      481 aagagcagta tctacgagca atttaagagc cggtgatctg gtattctatg gagatggtgg
      541 aagcattgat catgtggcac tttatatcgg cggtggtcag gtagtacatg caagtaattc
      601 cgcaccatat ccgcgaggag gaattaagat ttcaaacgta aattatagaa caccaatctg
      661 tgcaagacgt atcataggat agctagaaaa attaccaata cagtatttat ataggaagag
      721 cgtaaaagga agattcatgc agaatcattc ttgtgcgctc ttcctttttt gatataccag
      781 acatgaaaat aaaaaattat atttcctata ttgacagaag aacatcatgt ttatagaatg
      841 taaaaaggta tattttggtt aggaaaataa agatagaaaa gagatgtaaa aagaaaaaga
      901 tgaaacaaag acgatttaca aacaaaaagc tgatcgccct catgttacca ctggcagttg
      961 atcagctttt aaattcgttt atgggtacgg tagataccct tgtagtcagc aatttaggat
     1021 cagcagcgat ttctgctgtc agtctggtag attccattaa tattctggta gttcaggcat
     1081 tttttgcact ggcttcaggt ggtacggttg tttgctctca ttatcttgga tgtgagaaga
     1141 aagagagagc aacgaatgca gccagacagt tattgtttat tacattcgca atgtcattgg
     1201 tcattgcgat cgcgtgtcat ttattcagca accagcttct tggagtgatt tttgggcagg
     1261 tggaaccagc ggttatggac aatgcgaaga agtacttctt cttttcggcc atgtcttatc
     1321 catttatcgc attgtacgat gacggagcct gcattttaag agcacaggag aacagcaaac
     1381 ttccgatgca gatttctttt gttgcaaatg gcattaatgt tgcattgaat ctgatatttg
     1441 tatgggtatt gcatttgggt gtagcaggtt cttctgcagc gacgatgatc gccagagcat
     1501 ttgcaatggt tgcggtgctt tataaattga gaaatccgaa gatgaagata cagcttagag
     1561 attatttttc gattcgtcca gaatgggaag agatcaaaag aatcttacat atcggagtac
     1621 cctcaggaat tgaaaatgga atgttccagt ttggaaagct tgcgatccag tcaacggtat
     1681 ctttgatggg aacggcggcg atcgcagcac agggaatgac caatatcatc gagaacttga
     1741 atgggatcat gtcgatcgga atggggatcg gattgatgac agtcgttgga gaatgtcttg
     1801 gagcaggaga aaaagaagaa gcagtctatt atataaagaa gatttccatt gcagcagaag
     1861 ttgtattgat cgctacatgt cttttgatgt ttgggcttac ttatccgatc acatattttg
     1921 gaggcatgga accagagagt gcaaaacttt gtatattcat ggtaacatgt atcacgatcg
     1981 taaaaccgat cgtgtggatc atggcattta ttccaccata tggattcagg gcagcagggg
     2041 atgttagatt tacaatgacg acctccatgc tgtgtatgtg gttttgcaga gtcgtgcttg
     2101 ccatggtact tgcgagagtt tttcatatgg gaccgatcgc agtgtggatc ggaatgttta
     2161 cagactggac gatccgtgga attatttata caatacgatt taaaaataga aaatggcttg
     2221 ctcatcaggt gatctgatgt tcgttgcgga tgatgacggt tcgtgttata ctgaaatgta
     2281 tttgaatgat caagaaataa tatagaaggg atacaaataa aatgaaagta tcagatttta
     2341 catttgattt accagaagaa ttgatcgctc aggatccact tgaggacaga tcaagttcca
     2401 gattattaac attagataaa aatacaggag agatcggaca tgatgtcttc cataatattg
     2461 taaattattt aaaaccggga gactgtcttg tgttgaataa cacaaaagta attcctgcaa
     2521 gactgatcgg agccaaagaa gaaactggcg gtaaagttga agttttactt ctcaagagaa
     2581 aacaggacaa tgtatgggaa actcttgtaa agccaggaaa aaaagcacgt ccgggagcaa
     2641 ggatttcttt tggagatgga aaattagttg gagaagtcat tgatgttgtt gatgaaggaa
     2701 atcgtttggt gaagtttgaa tatgaaggta tttttgagga agtattagac gaacttggac
     2761 agatgccatt accaccatat atcacacatc aattaaaaga caagaatcgt tatcagacag
     2821 tttacgccaa atatgatggg tcagccgcag caccaactgc tggacttcat tttacaaagg
     2881 aattattaca gcagatcaaa gacatgggag ttaatatcgc atatgtaaca cttcacgtcg
     2941 gacttggaac attccgtcca gtaaaagttg atgatgtctt agaacatcat atgcattctg
     3001 aattttatag aattgaagag aaagatgcag aacttatcaa tgagacaaag aaaaacggtg
     3061 gaagagttat ttctgttgga acaacaagta caagaacttt ggaatctgtt gcagaagaag
     3121 atggaacact ccatgctaag agcggatgga cannnnnnnn nntgatcgat ggactgatca
     3181 caaacttcca tctgccagaa tctacattat taatgttagt ttctgcactc gcaggaagag
     3241 aacatatttt aaatgcttat aatatcgcag taaaagaaag atacagattc ttctctttcg
     3301 gggatgcgat gttcattcat ccataagaag aatttactat aaaaggatat cttagaaagg
     3361 agtcacaatg gtaaatttga tcgaaatagg aaaaagatat agaaacatta ttataagata
     3421 tgaaatggag gaaagcttat gaaac
//
LOCUS       DAOMUE010000218         3444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7781_length_3444_cov_2.061670, whole genome shotgun sequence.
ACCESSION   DAOMUE010000218 DAOMUE010000000
VERSION     DAOMUE010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7781_length_3444_cov_2.061670"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /locus_tag="PLC71_08835"
     CDS             complement(<1..1822)
                     /locus_tag="PLC71_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRM02784.1"
                     /translation="MKTFFKNIGNTIVKKLRKHPLICNFVLSVVLAFVLEVLGRQTFD
                     LSAVGFVDQRSKMFLYSIFIIFVTYSVTLITKRRLFSYIIVTLLWSIVGIVNFMMLSS
                     RNTPFTYVDITLMKSVLPVMNNYFSPLEIGAMGALLLIALVLLVVAYMYLPIEEHLNR
                     KSGFIKVIVIIAVFSGVTSYGFKSGMLVDQIHNIRIAFSDYGTPYCFSITALKNGIDK
                     PSDYSEKKIKKIEKRTQKKVAKMKKEKVKKPNIIFVQLESHFDITQVKGVKFNKDPLP
                     NFHKYMKGYSSGQLSMPSYGAGTANSEFELITGMNLDHFGAAEYPYKTVLQNTTTESM
                     ATVLKNYGYKAHAIHDNSAAFYDRDLVFSQLGFDTFTTKESMNIKKWTENGWAKDQIL
                     TGCIMDSLDSTKGQDYVYCISVQGHGDYPTTQIIENPEITVEGIEDEALKNKYTYYVN
                     QVYQMDQFVGKLVKALQQRGEPTILCMYGDHLPSLEIEDEDLTYGNKYQTSYFMWDNI
                     GLKKKDGTIEAYDLGSEVLNKCNIHTGLMNSFHQTRKGTKNYQKDMKELQYDMLYGKQ
                     YVWNQENPFKATDLQFGIRPLTVTKVYETKDSIFIVGNNFT"
     assembly_gap    1881..1890
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1999..>3444
                     /locus_tag="PLC71_08840"
     CDS             1999..>3444
                     /locus_tag="PLC71_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02785.1"
                     /translation="MSTNEKNRFSTLLEELISISGVKNSGLAKALQYDASYISKWVSG
                     RLLPTEKTKKKILSGLSHEIVSQSTSEGLENLYANYQVSFPEELENVIYDNLEIEYDY
                     VTDLQNTCGSLIAPDVSFFASLSPAQYITKMQHPVLRRVKMLDIMAEMDLLKLNHEYR
                     LQIMQGDVHRKLLHTYPDVHFSLVIDLSLNKIDDIYDSIFIMNMLSAMSQVDFYLYQN
                     QQASGRMIFTVKNDFMISALLVNPDRCMSVTISSNEKNCNPIYHGIKDRCTRESLLCR
                     RTSMKEMLSSHDYMHSILSLHSKWGIGHMTEHFLPDDLFHEILDLLKSSNDELFFDED
                     HLCYLHSMTQRTLAESEIDLFIHCQALSDLVVDGNLDFYDQKVHLTLSQRKKYFNHLL
                     HVFETNSNLTFKLAKEPMILDFKYQTNQCIFLSDQFSYIRLNTYNWANNLAIINHPNL
                     KHMFDRFFNAVWNDATSELLSDYSEIHSFFIH"
BASE COUNT         1048 a          711 c          511 g         1164 t
ORIGIN      
        1 tcgtaaaatt gtttcctaca ataaagatgc tgtcctttgt ctcatatacc tttgtcaccg
       61 taagcggccg gattccaaat tgaagatccg ttgccttaaa tggattctcc tgattccata
      121 catactgttt tccgtacaac atatcatact gaagctcttt catgtctttc tgataatttt
      181 ttgtaccttt tcttgtctgg tgaaaactgt tcattagtcc tgtatggata ttacacttat
      241 tcagaacttc tgatccaaga tcatacgcct cgatcgttcc gtccttcttc ttcaatccaa
      301 tattatccca cataaaataa cttgtctgat atttatttcc atatgtcaga tcttcatctt
      361 cgatctccaa acttggaaga tgatctccat acatacaaag aatcgttggt tctccacgct
      421 gttgtaatgc tttgaccagt ttcccgacaa attgatccat ctgataaacc tgattgacat
      481 aataagtata tttattcttc agtgcttcat cctcaattcc ttcaaccgtg atctccggat
      541 tctcgatgat ctgtgttgtt ggataatctc catgtccctg tacagaaata caataaacat
      601 aatcctgtcc cttcgtagaa tccaaactat ccataataca accggtcagg atctgatctt
      661 ttgcccatcc attttctgtc catttcttga tgttcatact ttcttttgtt gtaaatgtat
      721 caaaaccaag ctgtgagaat accagatctc gatcataaaa tgccgcactg ttatcatgga
      781 tcgcgtgtgc cttatatccg taattcttca ataccgtcgc catgctctct gtcgttgtat
      841 tctgaagcac tgttttatat ggatattctg ctgctccaaa atggtccaga ttcattcctg
      901 tgatcagttc aaactcagag tttgctgttc ctgccccata agacggcata gaaagctgtc
      961 cagaggaata ccctttcata tacttatgga aatttggtaa tggatctttg ttaaacttaa
     1021 ctcctttgac ctgcgtaata tcaaaatgtg attccaactg tacaaagatg atatttggtt
     1081 tctttacctt ttctttcttc atctttgcaa ccttcttctg tgttcgtttc tcaatctttt
     1141 tgatcttctt ttctgaataa tctgatggtt tatcaattcc attcttcaag gctgtaatac
     1201 tgaaacaata cggtgttcca taatcactga atgcaattcg gatattatgg atctgatcta
     1261 ccagcattcc actcttaaat ccataagaag taactcccga aaatactgct atgatcacta
     1321 taacttttat aaatccgctc ttgcggttta agtgttcttc tattggaaga tacatataag
     1381 ccactactaa caataccaat gcgatcagta acaatgctcc cattgctcca atctcaagtg
     1441 gcgagaagta attattcata actggcaata cagacttcat caacgtaata tctacatacg
     1501 taaatggagt atttcttgaa ctgagcatca taaaatttac gattccaacg atactccata
     1561 gcagtgttac tattatatag gaaaatagtc gtctcttagt aatcagtgtc acagagtatg
     1621 ttacgaaaat aataaagata ctatataaaa acatcttact tctctgatcc acaaatccca
     1681 cagcactaag atcaaatgtc tgacgtccca aaacttccaa tacaaacgcc agcacaactg
     1741 acaatacaaa attacatatc aatggatgtt ttcgaagctt ctttacgatc gtatttccga
     1801 tattcttaaa aaaagttttc atgcttttct cctttgctct ttttcgctat catagtatta
     1861 tatcattcaa aacagaaagt nnnnnnnnnn tcatatatta tcttaccact ttttgctatt
     1921 caattaaatc tttattaaat ctttttttgt gctatactaa agataacaaa gtatatctta
     1981 catacacgag gtaatcatat gtcaacaaat gaaaaaaaca gattcagcac cctgcttgaa
     2041 gaattaataa gtatctccgg tgtaaaaaat tccggtcttg caaaggcatt gcagtatgat
     2101 gccagttata tcagcaaatg ggtcagtgga cgcctgctcc caacagaaaa aacaaagaaa
     2161 aagattctct ctggtctaag tcatgaaatt gtaagccaaa gtacttctga aggacttgaa
     2221 aatttatatg ctaattacca ggtttcattt ccagaagaac ttgaaaatgt tatttatgat
     2281 aacttagaaa tcgaatatga ctatgttaca gatcttcaaa atacttgtgg ttccttaatc
     2341 gcacctgatg tgagcttctt tgcatcttta agccccgccc agtatattac aaagatgcag
     2401 caccctgtac ttagacgagt aaaaatgctt gatattatgg cagagatgga tcttttaaaa
     2461 ttaaaccacg aataccgctt acagattatg caaggtgatg tccatcgaaa gttattacat
     2521 acctacccgg atgttcattt ctctcttgtt attgacttgt ctcttaataa aattgatgat
     2581 atttatgatt caatcttcat aatgaacatg ctttctgcca tgtctcaggt ggatttttat
     2641 ttgtaccaga atcagcaagc ttctggacga atgatcttta cagtcaaaaa tgattttatg
     2701 atttccgctc ttcttgtaaa tcctgaccgt tgcatgtcag ttacaatcag cagtaatgag
     2761 aaaaactgca acccaatcta tcacggaata aaagacagat gtaccagaga atctttactc
     2821 tgccgaagaa caagcatgaa agagatgctt tcctctcacg attatatgca ttccatcttg
     2881 tctcttcatt caaaatgggg aattggtcac atgacagagc atttcctgcc agatgatctg
     2941 tttcatgaaa ttcttgattt gcttaaaagt agtaacgacg aattgttctt tgatgaagat
     3001 cacctttgtt atcttcattc tatgacacaa agaacactcg cagaatcaga aattgatctt
     3061 tttatccact gccaggcatt atcagatctt gtagttgatg gaaatcttga tttttacgac
     3121 cagaaagttc atcttacttt atcgcagaga aaaaagtact tcaatcattt actacatgta
     3181 tttgagacga attccaacct tacctttaag cttgcaaaag aaccaatgat ccttgatttc
     3241 aaatatcaaa ctaatcaatg tatctttttg tcggaccagt tttcttatat tcgattaaac
     3301 acatataact gggcaaataa tcttgcgatc attaatcatc caaaccttaa acatatgttt
     3361 gaccgtttct ttaacgcggt atggaatgat gcgactagtg aacttctctc tgattacagt
     3421 gaaatacatt catttttcat ccat
//
LOCUS       DAOMUE010000219         3434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7804_length_3434_cov_2.737200, whole genome shotgun sequence.
ACCESSION   DAOMUE010000219 DAOMUE010000000
VERSION     DAOMUE010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7804_length_3434_cov_2.737200"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(356..3151)
                     /locus_tag="PLC71_08845"
     CDS             complement(356..3151)
                     /locus_tag="PLC71_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HRM02786.1"
                     /translation="MLKREWQKISKNPWMIIILIAIITIPAIYTSVFLGSMWDPYGDA
                     DQLPVAVVNHDKKVNYEGKTLQVGDDLVKNLKDSGSLDFHFVSDKKAEEGLKSGEYYM
                     IISIPENFSKNATTLMDKNPKQMKLTYKTNPGTNYVASKMDDSAIAKIEKSVREKVTE
                     TYVKTVFDQIKTAGSGFQKAADGSKKIESGAKKLKAGNDTIEQNLKKLASSTLTFQNG
                     AKSLSVGLKTYTAGVAKVDSGAKSLKSGTKTLKNGVAALQTGATQLSNGSKSLKLGIS
                     QYTNGVSTAQAGSKQLAANNKALNAGVEKLSSGSKDLKDGTGTLSAGLKNLLGQTKET
                     SNGLGTQLKENEAKKDGIISKRSNFDTQLEAFNTKLNELNQMLSAGSTASETSTQTAN
                     NETAKKAASSAANAKSSVSDLNSKVSKLKNSSVLDKLSEEDKKALLAEIDEVDSAASD
                     VENNVDETYTNAQKAADTKTTAKTQSASSEQQLATVKAYVSSLAKSSKQLAESSQTLS
                     GYEKAMMNGLYEGLNEVKTTLDRQGTTQDTMGMIQALTQLSEGATKVQTGMTTLESGI
                     NDSTSGLKIGIANYTAGVESLNTGLTTLTNNSKALNSGATQLNSGIGQVSSKLPTFKS
                     GTAKLYKGTKTLKSGTAALVANNNTLNSGAGQLASGAGQIASGSSQLAAGSTTLGNGI
                     GTLQSGSKTLKDSLQKGADQVNSIKATKKTNKMFAAPVKAKNVEYSHVDNNGHAMAPY
                     MMSVGLFVACMAFTLMYPLMEKNEEVKSGLQWWLSKVTVMAAVSIAQAVIMVAVLMGI
                     NGLEPHYVGKTFGMAVLASMAFMSLICFGEMLLNRVGSYVMLVFMVVQLGAAGGTYPL
                     DMAPHFYTVLHKYMPFSYTVHAFRHTLSMDGQIGQDIAVFVGILVVSTLATIIFYRFR
                     MKKQVGGTLEPNGAPQH"
BASE COUNT          809 a          708 c          561 g         1356 t
ORIGIN      
        1 aatctgatca ctcttttagc gcattttggg attatttaat tttaatcgtt acagttgctt
       61 tctttccacg tacttttttc cgtggaatct tagtcttctt cataagtgaa agctccctat
      121 taacttatta tccctattta caattactat acttttttaa ttatactcct gcattaatct
      181 ttattcaatg tatttattca cattttatac acatttttat tacactcttc ttatatacaa
      241 cagcaaaaat aaaaagacca ggacagccag tttgcaatca tgtaaataca catcttccca
      301 aactgactgt cctagtcttt tgttcattag agtttttatt taattatttt tatatctaat
      361 gctgtggcgc tccattaggt tccaatgttc ctccaacctg tttcttcatt ctgaatcggt
      421 agaagatgat cgttgctaat gttgacacaa ctaagattcc aacaaatact gcaatatcct
      481 gtccgatctg tccatccata gatagtgtgt gacggaatgc gtgtactgta tagctgaatg
      541 gcatatattt gtgtaatact gtgtagaagt gtggtgccat atccagtgga tatgttcctc
      601 cggctgcacc taactgtacg accatgaata ctaacatgac gtaacttccg acacggttta
      661 ataacatctc accaaagcag atcaatgaca taaatgccat ggatgctaag acggccattc
      721 caaatgtttt tcctacgtaa tgtggttcga gtccattgat tcccattaaa actgcaacca
      781 tgatcactgc ctgtgcaatg gatactgctg ccatgactgt gactttgctt aaccaccact
      841 gaagtccact cttgacttct tcgtttttct ccattaaagg atacattaat gtaaatgcca
      901 tacatgctac gaataatcca acggacatca tatatggtgc cattgcgtga ccattgttat
      961 caacgtgtga atattctaca ttcttagctt ttactggtgc tgcaaacatt ttgtttgtct
     1021 tcttggttgc tttgatgcta ttaacctgat ctgctccttt ttgtaaggaa tcttttaatg
     1081 tcttgcttcc actctgaagt gttccgattc catttccaag agttgtagat cctgctgcta
     1141 actgacttga tccacttgcg atctgtccag ctccagatgc taactgtccg gctccactgt
     1201 ttaatgtatt gttgttagca actaaggctg ctgttcctga tttcagtgtt tttgttcctt
     1261 tgtaaagttt tgctgttcct gacttaaatg ttggaagttt tgagcttacc tgaccaattc
     1321 cactattcag ctgagttgct ccgctattta atgctttgga gttgtttgtt aatgttgtaa
     1381 gtcctgtgtt cagactttca actcctgctg tgtagttggc aattccaatc tttaatccgc
     1441 ttgtgctgtc gttgattcca gattctaagg ttgtcattcc tgtctgtact tttgtggcac
     1501 cttctgatag ctgagttaac gcctggatca tacccattgt atcctgagtt gttccctgac
     1561 ggtctaatgt tgtttttact tcatttaagc cttcatacag accattcatc atcgcttttt
     1621 cgtatccaga aagtgtctgg gatgattctg caagttgctt agatgatttt gctaaagaac
     1681 taacatatgc tttcactgtt gcaagttgct gttcactact tgcggactgt gtttttgctg
     1741 ttgtttttgt atctgccgct ttctgtgcat ttgtatatgt ttcatcaaca ttattttcaa
     1801 catcagatgc tgcgctatct acctcgtcga tttcagctaa taatgctttt ttatcttctt
     1861 cgcttaattt atcaagtaca gatgaattct ttaacttgct tactttgctg tttaaatcag
     1921 atacacttga ctttgcattt gctgcactgc ttgctgcctt cttagctgtt tcattgtttg
     1981 ctgtctgtgt tgatgtctct gatgcggttg atccagcaga aagcatttga tttaattcat
     2041 ttaattttgt attaaatgct tctaattgtg tatcgaaatt gcttcgtttg cttataattc
     2101 catctttctt cgcttcattt tcttttaact gagttccaag tccattggat gtttcttttg
     2161 tctgacccaa cagattttta aggcctgctg ataatgttcc agttccatct tttaaatcct
     2221 tacttccact ggataatttt tcaacacctg catttaatgc tttattattc gctgcaagct
     2281 gtttgcttcc agcctgtgct gtgctgactc cgtttgtgta ctgggagatt cctaatttta
     2341 aggatttaga gccattggat aactgagttg ctcctgtctg cagagctgca actccatttt
     2401 tcaatgtctt tgttcctgat tttaaagatt ttgctccact gtcaactttg gctacacctg
     2461 ctgtatatgt ttttagtcct acagaaagtg attttgctcc attctgaaat gttaatgtgc
     2521 tggatgctaa cttctttaaa ttctgttcga tcgtatcatt tccagctttc agcttcttcg
     2581 ctccgctttc gatctttttg gatccatctg ctgctttctg gaaaccgctt ccggctgtct
     2641 tgatctgatc aaatacggtc tttacataag tttctgttac tttttctctg actgacttct
     2701 caatcttcgc tatggcactg tcatccatct tggatgctac atagttcgta cctggatttg
     2761 tcttgtatgt cagtttcatc tgttttggat tcttatccat taatgttgtt gcattctttg
     2821 agaaattttc tggaatggaa atgatcatat agtattctcc actcttcaat ccttcctctg
     2881 ctttcttgtc acttacaaaa tgaaaatcca gtgatcctga atctttcaga ttctttacca
     2941 gatcatctcc tacttgtaat gtttttccct cgtaatttac cttcttatcg tgattgacta
     3001 ccgctactgg taattgatct gcatctccgt atggatccca catggaacca aggaatacag
     3061 atgtataaat tgcaggaatc gtaatgatcg cgatcaaaat gataatcatc caggggttct
     3121 ttgaaatttt ctgccattct cgttttaaca tgatcacagc tgctccttcc ttgcattttt
     3181 attacttata tagaggtcat acctctttga atcattggtt attatatgca cttccttgat
     3241 aaaagtcaat aatttattga acattggtca tttttttatt tcatacataa ctttcttttt
     3301 catttatgat atatcttttt tcttctgttc attttctttt gattctgtta tgataaaagt
     3361 gcttttttta tttgaatttt aatggaaacg aggaatttta ttacatatga tacaagatat
     3421 ttatccacat aaac
//
LOCUS       DAOMUE010000220         3434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_7805_length_3434_cov_2.478248, whole genome shotgun sequence.
ACCESSION   DAOMUE010000220 DAOMUE010000000
VERSION     DAOMUE010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7805_length_3434_cov_2.478248"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..439
                     /gene="rnpB"
                     /locus_tag="PLC71_08850"
     ncRNA           92..439
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLC71_08850"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            559..729
                     /locus_tag="PLC71_08855"
     CDS             559..729
                     /locus_tag="PLC71_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02787.1"
                     /translation="MKEIADIYIKMTIKWTNQTIRERTFKISKKLEINFKTDILKITK
                     AGKGGIKNESES"
     gene            713..1399
                     /locus_tag="PLC71_08860"
     CDS             713..1399
                     /locus_tag="PLC71_08860"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HRM02788.1"
                     /translation="MNPKVENLKGKLIVSCQALPDEPLHSSFIMGRMALAAKQGGASG
                     IRANTPEDIKEIKTQVDLPVIGIIKRNYDDCEIYITPTIKEIDELMEAKPEIIALDAT
                     ISSRPKGQKLDEFFHEIKEKYPDQLLMADCSTIEEVLHADELGFDFIGTTMVGYTKQS
                     KDLKIDENDFEILRTILSKVKHPVIAEGNINTPAKLKRVLELGAFCAVVGSAITRPQL
                     ITKSFANAIK"
     gene            1423..2244
                     /locus_tag="PLC71_08865"
     CDS             1423..2244
                     /locus_tag="PLC71_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRM02789.1"
                     /translation="MDYYVKSVIPIIESNYENFTALEKNIADFFMKNEEKMDFSAKEI
                     AKHLFVSEASLSRFAKKCGFRGYREFIYQYEESFEEKNKELTGHTRTVLNMYQELLNK
                     TYSLMDEAQIVRICEKLGQAKRVFVCGKGSSGLAAREMRLRFMRIGVDINSIQDPDLM
                     RMQAVFQKEGCLVIGISISGESEDVCYLLEESKKRGADTILITGKNKDDYEDICDELV
                     LIASRKYLDHGNVISPQFPILIMLDLIYSYYLEHDKKKKETMHDDTVRALKGERS"
     gene            2241..2969
                     /gene="nagB"
                     /locus_tag="PLC71_08870"
     CDS             2241..2969
                     /gene="nagB"
                     /locus_tag="PLC71_08870"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRM02790.1"
                     /translation="MRIIKTKSYEEMSKKAANIIASVVTLKPDCMLGLATGSSPIGTY
                     DELVKKYEAGDLDFSEVTTVNLDEYKGLPKENEQSYYYFMHEYLFDKVNINPEKTYLP
                     DGTNLNSEEEAARYEALVQSLGTVDLQLLGLGRNGHIGFNEPGDHFEDGTHCVDLKES
                     TIEANKRFFESADDVPKQAYSMGIGTIMRSKKILLVVSGEEKAQALKDSIYGPVTPEV
                     PGSILRFHPDVTIIADEAAMSLIK"
     gene            2989..>3434
                     /locus_tag="PLC71_08875"
     CDS             2989..>3434
                     /locus_tag="PLC71_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRM02791.1"
                     /translation="MKIQSIKTVYFSATGNTKNVVKLIGETIARNMNLSYKEIDFTLP
                     QAHKDSYEFTKEELVIFGTPVYAGRVPNKVLPMIQGLFQGNDAFAVPVVTFGNRNYDN
                     ALIELRNELENNHFHTIAAGAFVAQHAFTDQLATMRPGKSDQEEIRG"
BASE COUNT         1310 a          539 c          730 g          855 t
ORIGIN      
        1 ccaagcgaag caattccaaa gacatctctt ttaagagaat ttatcggtgg tagttgcttt
       61 gaagtgtaag atatgatata ataattggcg cttagtcgga caaatggtcg cgccgtttca
      121 gacgaaaggg aaacggtgag gaaagtccgg gcttcacagg gcaggatgcc ggataacgtc
      181 cggtaggggc aaccctcaag ctagtgcaac agaaatatac cgccacattc gtggtaaggg
      241 tgaaatggta aggtaagagc ttaccgcctg gctggtaaca ggcagggcat atgtaaacct
      301 catccgaagc aagaccgaga tgaaaacgat agatcggccc gatctgtttt caggtgggtc
      361 gctggagctt gctggtaaca gtaagtcgag atagatgacc attcaacgac ataacccggc
      421 ttatcgtctg gctaagcatt atgaataaga atgatagaga agttgtaaga gaaatcataa
      481 gtagatctta caacttcttt ttttgatatg taaaaagaag ttataacaaa tgtatttctt
      541 tcagaaacca gaaaaatctt gaaagaaatt gcagatatat atataaaaat gacgataaaa
      601 tggacaaatc aaactattcg tgaaagaact ttcaaaatat caaaaaaact tgaaattaat
      661 tttaaaacag atatactaaa gataacgaaa gcaggaaagg gaggaattaa aaatgaatcc
      721 gaaagttgag aatttaaaag gaaaactgat cgtatcctgc caggcattgc cagatgaacc
      781 attacattct tctttcatca tgggaagaat ggcacttgct gcaaaacagg gtggagcatc
      841 gggaatccgc gcaaatacac cagaggatat caaagagatc aaaacacagg tagatcttcc
      901 ggtgatcgga atcatcaaga gaaattacga tgactgtgag atttatatta caccaacgat
      961 caaagagatt gacgaattaa tggaagcgaa accagagatc atagcattag atgcaacaat
     1021 ttcaagccga ccaaagggac agaaattaga tgagttcttc catgagatca aagaaaaata
     1081 tccagatcaa ctactaatgg cagattgttc tacaatcgaa gaagtacttc atgcagatga
     1141 attaggattt gattttatcg gaacaacaat ggttggctat acaaaacaaa gcaaggatct
     1201 taaaattgat gaaaatgatt ttgaaatctt aagaacaatc ctttccaaag taaaacatcc
     1261 agtgatcgca gagggaaata tcaatacacc agcaaagtta aagagagttt tagaactagg
     1321 agcgttttgt gcagttgtag gttcagcaat cacaagacca cagcttatca caaaatcatt
     1381 tgcaaatgcc atcaaataaa agcattttta aaaggatcat gtatggatta ttatgtaaaa
     1441 tcggtcatac cgatcattga atcaaattat gaaaatttta cagctctgga aaaaaatatt
     1501 gcggatttct ttatgaaaaa tgaagaaaaa atggactttt cagcaaaaga gatcgcaaaa
     1561 cacttgtttg tttcagaagc ttcgttatca agatttgcaa aaaaatgtgg atttcgaggc
     1621 tatcgagaat ttatttatca atatgaggaa tcatttgaag agaaaaataa agaactcacg
     1681 ggacatacaa gaacagtgtt aaatatgtat caggagttgt taaataagac atacagcctg
     1741 atggacgaag cgcagatcgt aagaatctgt gagaaattag gacaggcaaa gagagtgttc
     1801 gtatgtggga agggaagttc cggccttgca gcgagagaga tgcgcctaag atttatgaga
     1861 attggagtag atatcaattc gattcaagat ccagatctga tgaggatgca ggcagtattc
     1921 caaaaggaag gttgtcttgt gatcgggatc agtatcagtg gagagagtga agatgtttgt
     1981 tatctattag aagaatcaaa aaaacgagga gcggacacca tcttgatcac aggaaaaaat
     2041 aaggatgact atgaggatat ctgtgacgaa ttagttttga tcgcatcaag aaaatattta
     2101 gatcatggaa atgttatctc tccgcagttt ccaatcctta ttatgttaga tcttatatat
     2161 tcttactatt tagaacacga taaaaagaaa aaagaaacca tgcacgatga cactgtgcgc
     2221 gcattaaaag gagaaagatc atgagaatca ttaaaacaaa atcatacgaa gaaatgagta
     2281 aaaaagcagc aaacatcatt gcatctgtag tcacattaaa gccagactgc atgttaggac
     2341 ttgcaacagg atcatcacca atcggaacat atgatgaatt agtaaagaaa tacgaagcag
     2401 gagatcttga tttttcagaa gttacaacag taaacttaga cgagtacaaa ggattaccaa
     2461 aagaaaatga acagagctat tactatttca tgcatgaata cttattcgat aaagtaaata
     2521 tcaatccaga aaaaacatat cttccagatg gaacaaactt aaacagtgaa gaggaagcag
     2581 cacgttatga agcattggta cagtctttag gaacggttga tcttcagtta ttaggattag
     2641 gaagaaacgg acatattgga ttcaacgagc caggggatca ttttgaagat ggaacacact
     2701 gcgtagactt aaaagaaagc acgatcgaag caaacaaacg attcttcgaa tcagcagatg
     2761 atgtaccaaa acaggcatac agcatgggaa tcggaacgat catgcgttct aagaagatct
     2821 tattagttgt cagcggagaa gaaaaagcac aggcattaaa agacagtatt tatggaccag
     2881 taacaccaga agtaccagga tctatcttaa gattccatcc agatgtaaca attattgcag
     2941 atgaagcagc tatgtcattg atcaaatagt tgaatatgtg gaataaaaat gaagattcaa
     3001 tctattaaaa cagtgtattt tagtgcgaca ggaaatacaa agaacgtcgt aaagttaata
     3061 ggagaaacaa ttgcaaggaa tatgaatctt tcatataaag agattgactt tacacttcca
     3121 caagctcata aagatagcta tgaatttaca aaagaagaac tagtgatctt tggaactcct
     3181 gtgtatgcgg gaagagttcc aaacaaagta cttccgatga tacaaggatt atttcaagga
     3241 aatgatgcat ttgcagtacc agttgttaca tttgggaaca gaaactatga caatgcattg
     3301 attgaattaa gaaatgaact agaaaacaat cattttcata cgattgcagc aggagcgttc
     3361 gtagcacagc atgcttttac agatcaatta gcaacgatgc gcccaggtaa gtcagatcag
     3421 gaagaaatca gagg
//
LOCUS       DAOMUE010000221         3367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8016_length_3367_cov_2.645531, whole genome shotgun sequence.
ACCESSION   DAOMUE010000221 DAOMUE010000000
VERSION     DAOMUE010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8016_length_3367_cov_2.645531"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1229)
                     /gene="spoIVA"
                     /locus_tag="PLC71_08880"
     CDS             complement(<1..>1229)
                     /gene="spoIVA"
                     /locus_tag="PLC71_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HRM02792.1"
                     /translation="EDENDRNRANDELPQSSSGKTIMTTEPKFVPNEAVSIKTEEGIE
                     LNVRLIDCVGYMVEGATGHMEGEEERLVKTPWFDYEIPFTKAAAIGTKKVITEHSTIG
                     VVVTCDGSFGEIAAKQYEPAEEETIKQLKALKKPFVVLLNTIHPYSESTKQLAAEKEE
                     KYQTKVLPMNLEQMKKEDIYEIIKSVLMEFPISSIGFYVPRWTEMLKKDHPLKMELLQ
                     MARDVITEKTTMRDIYEEQEKEYEYITGQKLESVAMDSGEVVITVKVGDVYYYEFLSE
                     TTGMEIRNEYEFIKIMGELAKKKKEYEEVGEALAAVKQRGYGVVTPTKEEIVLEEPQI
                     VKHGSKYGVKIKASAPSIHMIRANISTEIAPIVGEEYQAKDLMDYIEQGSNQPGESMW
                     DVNIFGKTLEQLVGDGM"
     assembly_gap    1230..1239
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1240..1343)
                     /locus_tag="PLC71_08885"
     CDS             complement(<1240..1343)
                     /locus_tag="PLC71_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02793.1"
                     /translation="MKARTNGEIYIGVVGPVRTGKSTFIKRFMNLMVLP"
     gene            complement(1483..2358)
                     /locus_tag="PLC71_08890"
     CDS             complement(1483..2358)
                     /locus_tag="PLC71_08890"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRM02794.1"
                     /translation="MEARISGTTKLFGLIGSPVSHSASPAMQNYGFQACGVDGAYLAF
                     DIKEEEVPEFVKSMRLLNIQGCNVTMPNKTAAAQNMDELSPAAELIGAVNTIVNRDGK
                     LYGHITDGEGFVANLKDHGIGVEGKDIVIFGAGGAATAIQVQCALDGAKSITIFNPRD
                     PFFERAQKMVEKIKAKVPECQVELYDLDAKDTMREKIDAADILVNGTLLGMKPKEDTT
                     VISDTTMFHEGLVVADVVYNPEETRFLREAKEHGCITVPGKGMLLWQGVASFKLFTGK
                     DMPVDEVKERFFQGV"
     gene            complement(2407..>3367)
                     /locus_tag="PLC71_08895"
     CDS             complement(2407..>3367)
                     /locus_tag="PLC71_08895"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRM02795.1"
                     /translation="ADAGLKNVVMEVSSQALMLHRTSGFEFDYGIFTNLAEDHIGENE
                     HKDMDDYIHCKSLLFKQCKQGIVNGDDDYVERIIKDHTCAIETYGMKDTNDVYAENIT
                     RIQEPGYLGVAYDLKGKQEYNVHVDIPGDFTVYNSLAAISLCRHMGIVKEDVLSALNT
                     IQVKGRLEIIKTPGDYTLMIDYAHNAMSLESLLTTIRKYNPKKIYCLFGCGGNRAKAR
                     RYEMGEVSSKLADLTVVTSDNPRNEEPMDIINDILIGVHKADGEYVTIPDRKEAIKYC
                     MEHAGAGDIVILAGKGHEDYQEIKGVKHHMDERDLIQEIIEGR"
BASE COUNT          852 a          781 c          475 g         1249 t
ORIGIN      
        1 tgcataccgt ctccaaccag ctgttctaac gtctttccaa aaatattgac atcccacata
       61 ctttcccctg gctgattcga tccctgctca atatagtcca tcagatcttt tgcctgatat
      121 tcttccccaa cgatcggagc gatctccgtt gaaatatttg cacggatcat gtgaatagaa
      181 ggtgcacttg ccttaatttt tactccgtat ttacttccat gttttacaat ctgcggttct
      241 tctaatacaa tttcttcttt cgtcggtgtc acaacaccat atcctctttg ttttactgcc
      301 gccaatgctt ctccaacttc ttcgtactct ttctttttct ttgctaattc tcccatgatc
      361 ttgataaatt catattcatt gcgaatctcc attcctgttg tttcacttaa aaattcataa
      421 taatatacat ccccaacttt tactgtgatg accacttccc cactgtccat cgcaacagat
      481 tctagtttct gccccgttat gtactcatat tctttttctt gttcttcata aatgtctctc
      541 attgttgttt tttctgtgat cacatctctg gccatttgaa gtaattccat ttttaatgga
      601 tgatctttct ttaacatctc tgtccatctt ggaacataaa atccaattga tgaaatcgga
      661 aattccatca acacactttt tataatttca taaatatcct cttttttcat ctgttctaaa
      721 ttcattggta gcacttttgt ctgatatttt tcttcctttt cagccgctag ctgctttgtg
      781 ctttctgaat atggatgaat cgtatttaat aaaacaacaa atggcttttt caatgctttc
      841 agttgtttga tcgtttcttc ttccgctggc tcatattgtt ttgctgcaat ttccccaaaa
      901 gaaccatcac aagttacaac tacaccgatc gttgaatgtt ccgtgatcac tttctttgtt
      961 ccaattgccg ctgctttcgt aaatggtatt tcataatcaa accaaggtgt ttttacaagc
     1021 ctctcttctt ctccttccat gtgtcctgta gcgccttcca ccatatatcc cacacaatcg
     1081 atcagtcgga catttaattc gattccttct tccgtcttta ttgatactgc ttcatttgga
     1141 acaaatttcg gttctgttgt catgatcgtt ttaccacttg aagattgtgg aagttcgtca
     1201 tttgcacgat ttctgtcgtt ttcatcttcn nnnnnnnnng gcaataccat cagattcata
     1261 aaacgtttga taaatgtact ctttcctgtt cgcaccggac caacaacacc aatgtaaatc
     1321 tctccgtttg tccttgcttt catatcctta tatacattaa actgatccat aagttcctcc
     1381 tttatagact gataaagtat atgaagacaa taagaaaaaa agaccagaaa tctgcttatt
     1441 acagtttctg atctttttgt ttccatatat gatctatgta tcttatactc cctggaagaa
     1501 tctttctttc acttcatcaa ccggcatatc tttccctgta aataatttga aagatgcaac
     1561 tccctgccat aatagcattc cttttcctgg aactgtgata catccatgtt cttttgcttc
     1621 tcttaagaat cttgtctctt ctggattata tacaacatca gctacaacta atccttcatg
     1681 aaacattgtt gtatctgaga tcactgttgt atcttctttt ggtttcattc caagtaatgt
     1741 tccattaact aaaatatctg ctgcatcaat cttttcacgc attgtatctt tcgcatcgag
     1801 atcatataat tctacctgac attctggtac cttagctttg atcttctcta ccatcttctg
     1861 ggctctctcg aaaaatggat ctcttgggtt aaagatcgtg atagattttg ctccatctaa
     1921 tgcacactgt acctgaatcg ctgttgctgc tccacctgct ccaaagatta cgatatcttt
     1981 tccttcaacg ccgattccat gatcttttaa gtttgcaaca aaaccttcac catctgtgat
     2041 atgtccgtat aattttccgt ctctgtttac aattgtgtta acggcaccga tcaattctgc
     2101 tgctggtgat aattcatcca tattctgagc tgctgctgtt ttatttggca ttgtaacatt
     2161 acatccctga atatttaaca gacgcatgga ttttacgaat tctggcactt cctcttcttt
     2221 aatatcaaag gcaagatacg ctccatcgac tccacatgcc tgaaaaccgt aattctgcat
     2281 tgctggtgat gcggaatgtg aaaccggtga accaatcaat ccgaataatt ttgttgttcc
     2341 tgaaattctt gcttccacgt ttgtaattct ccttcgtttc tttatatatt tcaaaagaca
     2401 gatgttttat cttccttcga taatttcctg gatcaaatct ctctcatcca tatgatgttt
     2461 cacacctttg atctcctgat agtcttcatg cccttttccg gcaagaataa caatatctcc
     2521 tgctccagca tgttccatac aatatttgat cgcctcttta cgatctggga tcgtaacata
     2581 ttctccatct gctttgtgaa ccccgatcaa aatatcgttg atgatatcca ttggttcttc
     2641 atttcttgga ttatcagatg ttacaacggt aagatctgca agttttgaag atacttctcc
     2701 catctcataa cgtcttgctt ttgcacggtt tccaccgcat ccaaataagc agtagatctt
     2761 cttagggtta tatttacgga tcgttgttaa aagactttca agactcatag cattatgtgc
     2821 atagtcgatc attaatgtgt aatctcctgg agtcttgatg atctccaaac gtcctttgac
     2881 ctgaattgta ttcaatgctg ataatacatc ttctttgaca attcccatat ggcggcaaag
     2941 agagattgct gccaaggaat tataaactgt aaagtctcct gggatatcta catgtacgtt
     3001 atattcctgt tttcctttga gatcgtaagc aactccaagg tatccaggtt cctgaattct
     3061 tgtaatattt tcagcataaa catcgtttgt atccttcatt ccatatgttt cgatcgcaca
     3121 tgtatgatct ttgatgattc tttctacata gtcatcatct ccattgacaa ttccttgttt
     3181 acactgctta aataacagac tcttacaatg aatatagtcg tccatatctt tatgttcatt
     3241 ttccccaata tggtcttctg caaggtttgt aaaaattcca taatcaaatt caaatccact
     3301 tgttctatga agcatcaatg cctgagaaga tacttccata acaacatttt tcaatcctgc
     3361 gtccgcc
//
LOCUS       DAOMUE010000222         3358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8042_length_3358_cov_2.744777, whole genome shotgun sequence.
ACCESSION   DAOMUE010000222 DAOMUE010000000
VERSION     DAOMUE010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8042_length_3358_cov_2.744777"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLC71_08900"
     CDS             complement(<1..187)
                     /locus_tag="PLC71_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRM02796.1"
                     /translation="MSRKKDISLMRKTIVAGNWKMNKTPSEAKALIEELKPLVANEEV
                     DVVFCVPAIDIIPVVEAA"
     gene            complement(233..1438)
                     /locus_tag="PLC71_08905"
     CDS             complement(233..1438)
                     /locus_tag="PLC71_08905"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRM02797.1"
                     /translation="MLNKKSVDDINVAGKKVLVRCDFNVPLKDGQITDENRLVAALPT
                     IKKLISDGGKVILCSHLGKPKGEPKPELSLAPVAVRLSELLGQEVKFAADDNVVGDNA
                     KAAVAAMKDGEVVLLQNTRYRAEETKNGEAFSKDLASLCDVFVNDAFGTAHRAHCSNV
                     GVTEYVDTAVVGYLMQKEIDFLGNAVNNPERPMVAILGGAKVADKLKVIDNLLEKCDT
                     LIIGGGMAYTFLKAQGLEVGKSLVDDEKIDYCKEMMAKAEKLGKNLYLPIDTTVADGF
                     PNPIDAEIEVSVVAADAIPADKEGLDIGPATCEKFAEAVKTAKTVVWNGPMGVFENPI
                     LAKGTIAVAQALADTDATTIIGGGDSAAAVNQLGFADKMTHISTGGGASLEFLEGKDL
                     PGVVAANDK"
     gene            complement(1603..2616)
                     /gene="gap"
                     /locus_tag="PLC71_08910"
     CDS             complement(1603..2616)
                     /gene="gap"
                     /locus_tag="PLC71_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRM02798.1"
                     /translation="MAVKVAINGFGRIGRLAFRQMFGAEGYEVVAINDLTSPKMLAHL
                     LKYDSSQGTYALADKVEAGENSITVDGKEITIYAEADAKNLPWGELGVDVVLECTGFY
                     TSKEKASAHIEAGARKVVISAPAGNDLPTIVYNVNHNELKAEDTVISAASCTTNCLAP
                     MAKALNDLAAIKSGIMCTIHAYTGDQMTLDGPQRKGDLRRSRAAAVNIVPNSTGAAKA
                     IGLVIPELNGKLIGSAQRVPTPTGSTTILTAVVEGNVTVDEVNAAMKAAQTESFGYNT
                     DEIVSSDIVGMRYGSLFDATQTMVLPLDNGTTEVQVVSWYDNENSYTSQMVRTIKYFA
                     ELA"
     gene            complement(2827..>3358)
                     /locus_tag="PLC71_08915"
     CDS             complement(2827..>3358)
                     /locus_tag="PLC71_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HRM02799.1"
                     /translation="QSKIDKVHNDEQVINHLEPMITEYLVKISHLAITEKEMKVVNNL
                     FDSITDIERSGDHVENMAGYLQHIKDMNENISEDAIEELRNMNNIVRAAFESAIRAIE
                     FDDTKEAKEALSYAEDVYCTRKIIRKRHIQRMKDGACSPDIGVYFLDIISSMERIAGY
                     AFTIARYILSQNDEEE"
BASE COUNT          884 a          711 c          544 g         1219 t
ORIGIN      
        1 tagcagcttc tacaaccggg atgatatcga ttgcaggtac gcagaatact acgtcaactt
       61 cttcgttagc tactaatggt tttaattctt cgattaatgc ttttgcctcg ctaggagttt
      121 tgttcatttt ccagtttcct gctacgattg ttttacgcat aagactaata tccttctttc
      181 ttgacatttt gtttataaag tgtattaaat attaaatatt taagtgcaat gattatttgt
      241 cgttggctgc tactacacct ggaaggtctt ttccttctaa gaattcaaga gaagctccac
      301 cacctgtaga gatgtgtgtc atcttgtctg caaatcctaa ctggttaaca gctgctgcag
      361 agtctcctcc accgatgatt gttgttgcat ctgtatcagc taaagcctga gcaacggcaa
      421 ttgttccttt ggcaaggata ggattttcaa atacacccat tggtccattc catacaactg
      481 ttttagcagt tttaactgct tctgcgaatt tctcacatgt agctggtcca atatcaagtc
      541 cttctttatc agctggaatt gcatcagctg ctacaacaga tacttcgatc tcagcatcga
      601 taggatttgg gaatccgtct gctactgttg tatcgattgg aagatataaa ttctttccta
      661 atttctctgc ttttgccatc atttctttac aatagtcgat tttttcatcg tctactaaag
      721 atttaccaac ttctaatccc tgtgctttta agaatgtata agccattcct ccaccgatga
      781 tcagtgtatc acatttttca agtaagttat caattacttt taatttgtca gcaactttcg
      841 ctccacctaa gatggctacc ataggtcttt ctggattatt aaccgcattt cctaagaaat
      901 cgatctcttt ctgcattaaa tatcctacaa cggctgtgtc aacatattct gttacaccaa
      961 cgtttgagca gtgtgctcta tgagctgtac caaacgcatc gtttacaaat acatcacaaa
     1021 ggcttgcaag atctttgctg aatgcttcac catttttagt ttcttctgct ctgtaacgag
     1081 tattctgaag aagtactact tctccatctt tcatagcagc tactgctgct tttgcattat
     1141 ctccgacaac attgtcatct gctgcaaatt taacttcctg tcctaacagt tcgcttaagc
     1201 gtactgctac tggtgctaaa gataattctg gttttggttc tcctttaggt tttcctaagt
     1261 gagagcaaag gattactttt ccaccgtcag aaatcagttt cttgattgtt ggaagtgctg
     1321 ctaccagacg attttcatct gtaatctgtc cgtcttttaa aggcacgttg aaatcgcaac
     1381 gtactaagac tttctttcct gctacattaa tgtcatctac agattttttg tttaacatgt
     1441 ttcgtttttc ctcctaagat tgtatgccat aaggcatatc ttaattgatc agggtttctc
     1501 aatccttgat taaaaatagg ggcccggtcc aaaagaccgg accccttatc tgagtcttat
     1561 tacactgtat aattacaggc ctgtcctcat tccaatatca aattaagcta attcagcaaa
     1621 gtatttgatt gttcttacca tctggcttgt gtaagagttt tcgttgtcat accaagatac
     1681 aacctgaacc tctgttgttc cattatctaa tggaagaacc attgtctgtg tagcatcgaa
     1741 taaagaacca tatctcattc ctacgatatc gctagatacg atttcgtctg tgttatatcc
     1801 gaaagattct gtctgagctg ctttcatagc tgcgtttact tcatcaactg ttacatttcc
     1861 ttcaacaact gctgttaaga ttgttgtaga tcctgtagga gttggaacac gctgagcaga
     1921 tccgattaat tttccgttta attctggaat aacaagtccg attgcttttg ctgcacctgt
     1981 gctgtttgga acgatattta cagctgctgc acgtgatctt cttaaatcac cttttctctg
     2041 tggtccgtca agagtcatct gatctcctgt gtaagcatgg attgtgcaca tgattccaga
     2101 tttgattgct gcaagatcat taagagcttt tgccataggt gctaagcagt ttgttgtaca
     2161 agatgcagca gaaataactg tatcctctgc ttttaattca ttgtggttta cattgtaaac
     2221 gattgttgga agatcgtttc ctgctggtgc agaaataaca acctttcttg ctcctgcttc
     2281 gatatgagca gatgcttttt ctttagatgt gtagaatcct gtacactcaa gtactacgtc
     2341 tactccaagt tctccccatg gaaggttttt agcatcagct tcagcgtaga ttgtgatttc
     2401 ttttccatca actgtgatag agttttcacc agcttctact ttatcagcta atgcatatgt
     2461 tccctgtgaa gaatcgtatt ttaataagtg tgcaagcatc tttggagatg ttaaatcgtt
     2521 gattgctaca acttcatatc cttctgctcc gaacatctgt ctgaatgcaa gacgtccaat
     2581 acgtccaaat ccattaattg ctactttaac tgccatctca aattcctcct aagtcttgat
     2641 taattaaatt ttgatggatt tgccaatctg ttaatctatt gacaaacttc ctttttattt
     2701 taaacaatac tgacaaaaaa ttcaagtgaa atttagtatt tttatcatgt atacaataaa
     2761 acgattttgt acaattctac caaattttat cccgtttctt cggcacaatg aataaaaaac
     2821 ttttccttat tcttcttcat cattctgact taaaatgtat cttgcaattg taaatgcata
     2881 tcctgcgatt ctttccatac tggaaatgat atcaaggaaa tatacaccaa tatctggaga
     2941 acaagctcca tctttcattc tctgaatatg tctctttctg atgatctttc ttgtacaata
     3001 tacatcttct gcataagata atgcttcttt tgcttctttg gtatcgtcaa attcaatcgc
     3061 acggattgct gactcaaatg cagctcttac aatattattc atatttctaa gctcttcaat
     3121 cgcatcttct gaaatatttt cattcatatc cttgatatgc tgcagatatc ctgccatatt
     3181 ctctacatga tctcctgaac gctcaatatc tgtgatcgaa tcaaacagat tgttgacaac
     3241 tttcatctct ttttctgtga tcgccaaatg actgatcttt acaagatatt ctgtgatcat
     3301 tggttctaaa tggttgatca cctgttcatc attatgaact ttatcgatct tactctgt
//
LOCUS       DAOMUE010000223         3351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8060_length_3351_cov_2.247269, whole genome shotgun sequence.
ACCESSION   DAOMUE010000223 DAOMUE010000000
VERSION     DAOMUE010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8060_length_3351_cov_2.247269"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="PLC71_08920"
     CDS             <1..928
                     /locus_tag="PLC71_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRM02800.1"
                     /translation="NKGLELYKENGCDFLVALGGGSPIDSMKAIAALVANGGNISDYM
                     GKIIGGELPTTVAIPTTAGTGSEATQFTIITDTQKDVKMLLKGAVLMPDYAIIDPQFT
                     MTAPPKITAATGLDALTHAAEAYTSKLAQPLSDTFALSAIKRIFKYLPVAFHDGKNVE
                     AREQMSIAALEAGIAFNNASVTLVHGMSRPIGALFHVAHGLSNAMLLKQCFIYALDGA
                     YDRFADLGRAIGAANDADSDKEASEKFLQAVVDICDELETPTLEEYGINKEEFFNVID
                     KMAADAMESGSPSNTRKAITAEDVKEIYKNLW"
     gene            complement(1140..2018)
                     /locus_tag="PLC71_08925"
     CDS             complement(1140..2018)
                     /locus_tag="PLC71_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02801.1"
                     /translation="MKRNVYRILGCFLFAFTLCIMTPSFVKASVKNIPQTKTSGTYVG
                     NVDLTGDENADSVIIRTTPDQEGWYINRFTIYLNGKRTTEISLRDHDCYDLTVKYAKM
                     SKQHTFIQIIGRGENDYVTYNEIFTYNKKSNQFRVVKSFNNRSSYAEEIVTANKKGIT
                     VKHRVQPMETGWINWTLPFKYSKQKFIRTASSTKTVRSELGQNRKDKYSRYFAKNKFI
                     TAKKVTFYKKAGSKKVAFRVPKGKAVTLKKLTYSKKKIYLQFKYGKKYGYLRVNRANY
                     NFEKPLFQNVNSRLAG"
     gene            2348..2830
                     /locus_tag="PLC71_08930"
     CDS             2348..2830
                     /locus_tag="PLC71_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HRM02802.1"
                     /translation="MPQNKRESLIYTFIMCFMMVFWMSIYNVSLHMGGLSMESIKAGW
                     LGFPIAYIFAMCCDWFFVSKIAKGFAFKYLVKPDSSVLRKVISVSCCMVVPMVIIMSL
                     YGAVEVSIEMGNFSNLLIMWLTNIPKNFVMALPYQLIIAGPVVRFVFRAAFPEGKVLA
                     "
     gene            2934..>3351
                     /locus_tag="PLC71_08935"
     CDS             2934..>3351
                     /locus_tag="PLC71_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HRM02803.1"
                     /translation="MKNNVKNLIEFIDHSPSCFHVIENARKQFLEHGFMELKEKEYWD
                     LEEGKSYFVTRNGSSIIAFHLPKKDFQGFHIVASHSDSPTYRIKEQPEMSVEKHYTKL
                     NIERYGGMIPETWFDRPLSVAGRIIIEENGQIKDQLV"
BASE COUNT         1171 a          524 c          638 g         1018 t
ORIGIN      
        1 caataaagga ttagagcttt acaaagaaaa cggatgcgat ttcttagttg cactaggtgg
       61 aggaagtccg atcgactcta tgaaagccat cgcagcactc gttgcaaatg gtggaaatat
      121 ctccgactat atgggaaaga tcattggtgg ggaacttcca acaactgttg caatcccaac
      181 aacagcaggt acgggatcag aagcaacaca gtttacgatc attacagata cacaaaaaga
      241 tgtaaaaatg ttattaaaag gtgcagtgtt aatgccagat tatgcaatca ttgatccaca
      301 gtttacaatg actgcaccac caaagatcac agcagcaaca ggattagatg cactgacaca
      361 tgcagcagaa gcgtatacat ccaaacttgc acagccatta tctgatacat ttgcattatc
      421 agcgatcaaa agaatcttta aatacctgcc agttgcattc catgatggaa agaatgtaga
      481 ggcaagagaa cagatgtcaa tcgcagcgtt agaagcagga atcgcattta acaatgcatc
      541 tgttacatta gttcatggaa tgagccgtcc gatcggagca ttattccatg tagcgcatgg
      601 attatccaat gcgatgttat taaaacaatg cttcatctat gctttagatg gtgcttatga
      661 tagatttgca gatctaggaa gagcgattgg agctgcaaat gatgcggatt ctgataaaga
      721 agcaagtgaa aaattccttc aggcagttgt tgatatctgt gatgaacttg aaacaccaac
      781 actggaagaa tatggaatca acaaagaaga attctttaat gtgatcgata aaatggcagc
      841 agatgctatg gaaagtggaa gcccatccaa tacaagaaaa gcaatcacag cagaagatgt
      901 aaaagaaatt tataaaaatt tatggtagat gttataaaat tgccaaactc ctaagaaaca
      961 tataacataa acaaagataa tcataaatga aaactccaaa aatataaaaa ataaaactcc
     1021 aaaaaacata taaaactcaa acacatataa ttcgaaaaat ctaaaaagaa aagaacaacc
     1081 atattatgaa cggatcaaaa tggtggttct tttctttttt tggttggtat tgaaaatgat
     1141 cagccagcta atcgtgaatt aacattctga aaaagtggtt tttcaaagtt ataatttgcc
     1201 cgatttacac gaagatagcc atatttcttc ccatatttaa actgtaaata aatctttttc
     1261 ttagaataag ttaatttctt taaagttaca gctttccctt ttggtacacg aaaagcaact
     1321 tttttgctgc cagctttttt ataaaaagtt acttttttgg cagtgataaa cttatttttt
     1381 gcaaaatatc ttgaatattt atcttttctg ttttgtccaa gttcgcttcg taccgttttt
     1441 gtagaagatg cagttctaat gaatttttgc ttactgtatt taaaaggcag tgtccaattg
     1501 atccatccgg tttccatagg ttgtacacga tgtttaacgg taatcccttt tttattggca
     1561 gtaacgattt cttcagcgta agatgatcga ttattaaaag attttacaac gcgaaattgg
     1621 ttggattttt tgttataagt aaagatttca ttataagtaa cgtagtcatt ttcaccacgg
     1681 ccaataatct ggataaatgt atgttgtttg ctcatttttg catattttac agtgaggtca
     1741 taacagtcgt gatcacggag agatatttct gtggtccttt ttccatttag atagatcgta
     1801 aaacggttga tgtaccagcc ttcttgatcg ggtgttgtac ggatgatcac agaatcagca
     1861 ttttcgtctc cagtgaggtc aacattacca acataagttc cagaagtttt tgtttgaggt
     1921 atgtttttaa cagaagcttt tacaaatgat ggtgtcataa tgcataatgt aaaagcaaaa
     1981 agaaaacaac ctaaaatcct gtatacattt cttttcataa gaaaatccct ccttcttaag
     2041 tatatgaaaa ctaattgttt ctaattacat tttaacacat ttcattgtgg aaaatgcaca
     2101 aaaatatggt atgtataata attaaattaa agaatgtgac gaaattaaaa aaagtgctta
     2161 tacaagacat aaaaagtgtg tataataatg cattgacaag aaataagcag gaaagaacgc
     2221 agtgaagatt gcacacgata gaaagtggag atccgcaacg agtttggttg agggtctcca
     2281 ctattttttt gccttatttc ttgaagaaaa gcaacaattt atcacaacaa gaagaggaga
     2341 aaagaaaatg ccacagaata aaagagaaag ccttatctat acattcatca tgtgttttat
     2401 gatggtattt tggatgagca tatacaacgt atcattacat atgggaggat tatcaatgga
     2461 atccatcaaa gccggatggt tagggttccc gatcgcatat atttttgcaa tgtgctgcga
     2521 ctggttcttt gtatctaaga tcgcaaaagg atttgcgttt aagtatcttg taaaaccaga
     2581 cagctctgtt ttaagaaaag ttatttcagt atcttgctgt atggttgtac ctatggtgat
     2641 cattatgtca ctttatggag cagtcgaagt tagtatcgag atggggaatt tctcaaactt
     2701 actgataatg tggcttacaa atattccaaa gaactttgta atggcacttc cttatcagtt
     2761 gatcattgca ggaccagttg tgcgctttgt gtttagagct gcatttccgg aaggaaaagt
     2821 gcttgcataa taaaagaata taacagatat atcaaactga cataaccaca tttcttttct
     2881 ttcctatata aatgtgctag aataatcgtt agaaaatttt tggagggaaa tagatgaaaa
     2941 acaatgtcaa aaatttaatc gaatttattg atcatagtcc aagctgtttt catgtgatcg
     3001 agaatgcaag aaaacagttt ctggaacatg gttttatgga attaaaagag aaagaatatt
     3061 gggatctgga ggaaggaaag agttactttg ttacgagaaa cggaagttct atcatagcat
     3121 ttcatttgcc aaagaaggat tttcaaggat ttcatatcgt ggcaagtcac agtgattctc
     3181 caacatacag gatcaaagaa cagcccgaga tgtcagttga gaaacattat acgaaattaa
     3241 atattgaacg ttatggtgga atgattccag aaacatggtt tgatcgtcca ttatctgttg
     3301 cgggacgtat tataatagaa gaaaacggac agatcaaaga tcagttggtc a
//
LOCUS       DAOMUE010000224         3350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8064_length_3350_cov_2.162671, whole genome shotgun sequence.
ACCESSION   DAOMUE010000224 DAOMUE010000000
VERSION     DAOMUE010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8064_length_3350_cov_2.162671"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="PLC71_08940"
     CDS             <1..1014
                     /locus_tag="PLC71_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02804.1"
                     /translation="ITQGLYALFFWYGMRYAYKHKTYDYRSFSDSLYGKTRPIMSNLY
                     EICYLIMIGTASAAAFATGGSTLQMLFGIPYWVCTLIIAAFIFVIALFGTSVVRKCAS
                     TLSVLILIGLVLVLVPNIIVQWDAITAAISNLVSGKMPVISGESGAFGPALYSAVLYF
                     FFQLASVSVMYQHMEPVTDKKQINKAAIEIFVFNFFAMELSIIGLLAVAYVADLVTAT
                     VPMLVLVQNGVSSGLLTPIISVLIILGSISTAVNMISGIVTRCVNAVERRMRSEQERQ
                     NGHLVRNAIATLIFTFLAFAIAQFGLLAVVKQGYAYLGYAAFITLFIPFIIHVIVTKG
                     KEI"
     regulatory      1168..1273
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1442..>1799
                     /locus_tag="PLC71_08945"
     CDS             1442..>1799
                     /locus_tag="PLC71_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HRM02805.1"
                     /translation="MRTALSIAGSDSSGGAGVQADLKTMTMNGVFAMSAITALTAQNT
                     TGVTGILEADPEFLKKQIDAVFEDIRPDAVKIGMVSSSGLIKTIAERLQHYKAENIVV
                     DPVMVATSGSRLLEEDA"
     assembly_gap    1800..1809
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1810..2248
                     /locus_tag="PLC71_08950"
     CDS             <1810..2248
                     /locus_tag="PLC71_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HRM02806.1"
                     /translation="LKKELLPIATVITPNIPEAEILCGMEIHTEEDMVAAAKAIYEDL
                     GCAVLLKGGHNINDANDLLYTKEEVSWFKGKRINNPNTHGTGCTLSSAIAANLAKGFD
                     LKISVQRAKDYISRALADMLDLGAGSGPMNHAFDLKDEWKEEA"
     gene            2253..>3350
                     /gene="cytX"
                     /locus_tag="PLC71_08955"
     CDS             2253..>3350
                     /gene="cytX"
                     /locus_tag="PLC71_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HRM02807.1"
                     /translation="MGNRRTSIFENGLIWFGAGVSLAEILTGTYFASLGFSTGLLAII
                     IGHIIGCGMMFFAGFIGGKTRKSAMETVKMSFGSTGALLFAVLNILQLVGWTAIMIYD
                     GSLAAAGIFNIANWVWPVIIGGLIIIWILVGIENLGKINTVAMAALFILTLVLSFIIF
                     GHGSLKPVANDGLTFGAAVELAVAMPLSWLPLISDYTREAKEPTKATWASVIVYGLVS
                     CWMYVIGMGISIFTGESDIAQIMVKAGLGIAGLLIIVFSTVTTTFLDAWSAGISSESL
                     SKNINGKWIAVAATVIGMIGAIVYPMDDITDFLYLIGSVFAPMIAVQIADFFILHQDF
                     SRKSVCIQNMIVWVIGFIAYRWLMGVDTILGN"
BASE COUNT         1089 a          542 c          736 g          973 t
ORIGIN      
        1 atcacacaag gattatatgc attatttttc tggtatggaa tgcgttatgc atacaaacat
       61 aaaacatatg attacagaag tttttctgac agtttatatg gaaagacaag accgatcatg
      121 tcaaatctat atgaaatctg ttatctgatc atgatcggaa cggcatccgc tgcagcattt
      181 gcaacaggcg gatcaacact tcaaatgcta tttggaatcc catactgggt atgtacattg
      241 atcatagcag catttatttt tgtgatcgca ctctttggaa caagtgtcgt aagaaaatgt
      301 gcatctactt taagtgtact gatcttgatc ggattagttc ttgtattagt accaaacatc
      361 atcgtacagt gggatgcgat cacagcagca atctctaact tagtatcagg aaagatgcct
      421 gtgatctctg gagaaagcgg agcttttgga ccagccttat attctgcagt tttatatttc
      481 ttcttccagt tagcatctgt atctgttatg taccaacata tggagcctgt tacagataag
      541 aaacagatca ataaagcagc cattgaaatc tttgtattta acttctttgc aatggaattg
      601 tctatcattg gattattagc ggttgcatac gtagcagacc ttgtaacagc aacagttcct
      661 atgttagttt tagtacaaaa tggagtatct tcaggattat taacaccgat catctctgtt
      721 ttgatcatcc ttggatctat ttcaacggcg gtaaacatga tctctggaat cgtaacaaga
      781 tgtgtcaatg cagtagagcg tcgtatgaga tctgagcagg aaagacagaa tggacattta
      841 gtaagaaatg ccattgcaac actgatcttt acatttttag catttgcgat cgcacagttt
      901 ggattgttgg ctgttgtaaa acagggatat gcgtaccttg gatatgcagc atttatcaca
      961 ttgtttattc catttatcat tcatgtgatc gtgacaaaag gaaaagagat ttaaaaatta
     1021 tttttgggga aagaatttat tatgaaaaag ttagggttaa cgatatttcc aagaaaggca
     1081 agaaaaataa gctaaaaatt aaaaaaagca ataaagtacg aaaatagaaa cttgcttttt
     1141 agagaaactt gctatatact tattaagtta taaaaacagg gagcttgtta aacaggctga
     1201 gaggaaagta aaactttcga cctttgaacc tgatttgggt aatgccaacg taggaaacga
     1261 agatgataag tcgcgaacat acggaaagtt acaaaatcag tagctttccg tttttttcgt
     1321 atgataaagg attcctatta tggaagaatt tacaatgata cgaaacaatg cggtcaactg
     1381 ggagcaccac ttttgatcgc aatagaaaaa taatgcataa gagagaaaag gagagatact
     1441 tatgagaaca gcattatcaa tcgctggaag tgattccagt ggaggcgctg gagttcaggc
     1501 tgacttaaag accatgacaa tgaatggtgt atttgcaatg agtgcgatca cagcactgac
     1561 ggcccagaat acaaccggcg taacaggaat attagaagca gatccagaat tcttaaagaa
     1621 acagatcgac gcagtttttg aagacatcag acccgatgca gtcaagatcg ggatggtttc
     1681 ctcaagcgga ctgatcaaaa cgatcgcaga aagattacag cattataaag cggaaaatat
     1741 cgttgttgac ccagtgatgg tagcaacaag cggatcacga ttattagaag aagatgccgn
     1801 nnnnnnnnna ttaaagaaag aattattacc gatcgctacg gtaattacac cgaatattcc
     1861 agaagctgag attctttgtg gaatggagat tcacacagaa gaagacatgg tagcggctgc
     1921 aaaagcgatc tatgaagatc ttggatgcgc agtgttatta aaaggtggtc ataatattaa
     1981 cgatgcaaat gaccttttat atacaaaaga agaagtttcc tggtttaaag gaaaaagaat
     2041 caataaccca aatacacatg gaacaggatg cactctttca agtgctatcg cagcaaatct
     2101 tgccaaaggt tttgacctta agatttctgt acagcgtgca aaagactata tttcaagagc
     2161 attggcagat atgttagatc ttggagctgg aagcggacca atgaatcatg catttgattt
     2221 aaaagatgaa tggaaagagg aggcataaat aaatgggaaa tagaagaaca tcaatttttg
     2281 aaaacggact gatctggttt ggagccggag tgtccttggc agaaatttta acaggtacat
     2341 atttcgcatc cttaggattt tctacaggat tattagcaat catcattgga catatcattg
     2401 gatgtggtat gatgtttttt gccggattta tcggtggaaa gacaagaaaa agtgcaatgg
     2461 aaacggtaaa aatgagtttt ggatcaacgg gagctttgtt atttgcagtg ttaaatatcc
     2521 tgcagttagt tggatggaca gcgatcatga tctatgacgg atcattagca gcagcaggaa
     2581 tcttcaatat tgcgaactgg gtatggccag tcattatcgg aggattgatc attatctgga
     2641 ttctggtagg aattgaaaat ctaggaaaga tcaatacggt tgcaatggca gcattattta
     2701 ttttgacatt agttttaagt tttattattt tcggacatgg ttcattaaag ccggttgcaa
     2761 atgatggatt aactttcgga gcagctgtag aacttgcagt tgccatgcca ttatcatggc
     2821 tgccactgat cagtgactac acaagagaag ctaaagaacc aacgaaagca acatgggcaa
     2881 gtgtgattgt ttatggattg gtcagctgct ggatgtatgt gatcggtatg ggaatctcta
     2941 tctttacagg agaatctgat atcgcacaga tcatggtaaa agcaggactt ggaattgcag
     3001 gattattaat tatcgtattc tcaactgtaa caacaacatt cttagatgct tggtctgccg
     3061 gaatttccag tgaatcccta tctaagaata tcaatggaaa atggatcgct gtcgcggcaa
     3121 cagtgatcgg aatgattggg gcaattgttt atccgatgga cgatatcaca gatttcttat
     3181 atctgatcgg atcagtcttt gcaccaatga tcgctgtaca gatcgcagat ttctttatcc
     3241 ttcatcagga cttctcaaga aaatctgtat gtattcagaa tatgatcgta tgggtaattg
     3301 gatttatcgc atatcgctgg ctgatgggtg tcgatacgat tcttggaaac
//
LOCUS       DAOMUE010000225         3340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8099_length_3340_cov_2.538508, whole genome shotgun sequence.
ACCESSION   DAOMUE010000225 DAOMUE010000000
VERSION     DAOMUE010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8099_length_3340_cov_2.538508"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /gene="ulaG"
                     /locus_tag="PLC71_08960"
     CDS             <1..1078
                     /gene="ulaG"
                     /locus_tag="PLC71_08960"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HRM02808.1"
                     /translation="ESWIESTFPEWGTWLVEDIENEVVAPENVAMWWLGCTGVWFKTP
                     ADTNITIDLWCGNGKRTHGDGKMKVGHQMANMCGGRAMQPNLRNVPFVIDPFAFKKVD
                     AVLATHYHQDHMSAEWAAHVINSGMTTTDENGKEIPVPFIGPKKSVELWQKWGVPADR
                     CITVKPGDSIKIKDIEIIALDSFDRTCIVTTDSTGPDREELTGVCPTDMDDKAVNYLV
                     KTPGGNIYHSGDSHFSIYFAKHGKDYDVDVAFGSFGENPIGMQDKMTSIDVLRMAENL
                     QCKVVVPIHWDVWTNFQADCDEIKVLYDFKKDRNEYKFHPFFWQVGGKYTYPADKDKI
                     YYHHRRGFEDCFEAPQNIPFRSCL"
     gene            1094..2143
                     /locus_tag="PLC71_08965"
     CDS             1094..2143
                     /locus_tag="PLC71_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02809.1"
                     /translation="MKVSKKVIGNLEKCYSVAPLTYKGKKHMLVAAEKVNQCRLYDLE
                     GNQEEVIWEGPGGTMSMVQVPGSDGQFLATHKFYSPNDSKEAKIILAYPEDGEWKVRT
                     LAELPFVHRFDIISRNGVNYVIACTLKSGHEYKEDWRSPGKIQVCVLPEDLSSVDEEH
                     PLQFEVLKEGLLKNHGYCKAEVDGVLRSYVAANEGVFECIPPESEEGTWEIKQILDEA
                     SSDMAFADFDNDGELEMLTISPFHGEKISIFKKQDGEFKKVYTYEKDAEFAHGIWGGE
                     IAGTQGVLIGHRKGERNLLFFRCTDPEKLTFTADLLDSDIGPANVLHYTNDGEDYILS
                     ANREIDEIALYKVEK"
     assembly_gap    2316..2325
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2326..3223
                     /locus_tag="PLC71_08970"
     CDS             <2326..3223
                     /locus_tag="PLC71_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HRM02810.1"
                     /translation="LYYNAEMTQNQIAERFFTSRSKISRMLKEARQLGIVEIKILEPW
                     DRNVELEQEFMRRFALKDVRVISVKEANNTMVLQKLGEVAAYYLDNLLNDNMILGISW
                     GNTMYHTVKAVKTSKNIPITVVPIMGAANVRTPERDSLDLSKELAYAYGGTYHYIYAP
                     LFVNSEEVRDSLEQEPNIKGCLELARNADIILTSVGSIVYKSWKSYLSTRDLYNLEKK
                     GAIGHIGGHFYDMEGNEVITPFVNKMIGLGIEDIKNTENVICVAGLEMKAEAILGAVR
                     GGYINTLITDEEAAKAVLALCK"
BASE COUNT         1178 a          513 c          754 g          885 t
ORIGIN      
        1 agaaagctgg attgaaagca cattcccaga gtggggaaca tggttagtag aagacattga
       61 aaatgaagta gtagcaccag aaaatgtagc aatgtggtgg cttggatgta caggtgtatg
      121 gtttaaaaca ccagctgaca caaacattac gatcgactta tggtgtggaa atgggaaacg
      181 tacacatggt gatggaaaaa tgaaagtcgg acatcagatg gcaaatatgt gtggtggacg
      241 tgcaatgcag ccaaaccttc gtaatgtacc atttgtaatt gatccatttg catttaagaa
      301 agttgatgca gtattagcaa cacattatca tcaggatcat atgtctgcag aatgggcagc
      361 acacgtgatc aacagtggaa tgacaacaac agatgaaaac ggaaaagaaa ttccagtacc
      421 attcatcgga cctaagaaat ccgtagaatt atggcagaaa tggggagttc cagcagaccg
      481 ttgtatcaca gtaaaacctg gagattccat caagattaaa gatatcgaga tcattgcgtt
      541 agattccttt gacagaacat gtatcgtaac aacagactct acaggtccag acagagaaga
      601 attaacaggt gtatgcccaa cagatatgga tgacaaagca gttaactact tagttaagac
      661 accaggtgga aatatctatc attctggaga ttctcacttc tctatctatt ttgcaaaaca
      721 tggaaaagat tacgatgtag acgtagcatt cggatctttc ggagaaaacc ctatcggtat
      781 gcaggataag atgacatcca tcgatgtatt acgtatggct gaaaacttac agtgcaaagt
      841 tgttgtacct attcactggg atgtatggac aaacttccag gctgactgtg atgagattaa
      901 agtattatac gacttcaaga aagatcgtaa tgaatataaa ttccatccat tcttctggca
      961 ggttggagga aaatatacat atcctgcaga taaagataaa atctactatc atcacagaag
     1021 aggattcgaa gactgctttg aagcaccaca gaacatccca ttccgttctt gcttatagga
     1081 agaaggtggc ataatgaaag tatcaaagaa agttattgga aacttagaaa aatgctattc
     1141 tgttgcgcct ttaacttaca aaggaaagaa acatatgctc gtagcagcag aaaaagtaaa
     1201 ccagtgccgt ttatatgatc ttgaaggaaa tcaggaagaa gtaatctggg aaggaccggg
     1261 aggaaccatg tcaatggttc aggtccctgg ttccgacgga cagtttttag cgacacataa
     1321 attctactct ccaaatgatt ccaaagaagc aaagatcatc ttagcttacc cagaagacgg
     1381 agaatggaaa gtaagaacac tagcagagct tccatttgtg catcgttttg atatcatttc
     1441 aagaaatgga gtaaactatg tgatcgcatg tactttaaaa tcaggacatg aatacaaaga
     1501 agactggaga agtccaggaa agattcaggt atgcgttctt ccagaagatt taagtagtgt
     1561 tgatgaagaa catccattac agtttgaagt attaaaagaa ggtttattaa agaatcatgg
     1621 atactgtaaa gcagaagttg atggagtttt aagatcttat gttgcagcaa atgaaggtgt
     1681 ttttgaatgt ataccaccag aatcagaaga aggaacatgg gaaatcaaac agatcttaga
     1741 tgaagcaagc agtgatatgg catttgcaga ctttgacaat gatggtgaat tagaaatgtt
     1801 aacaatctca ccattccatg gagagaagat cagcattttc aagaaacagg atggagaatt
     1861 caagaaagtc tatacttatg aaaaagatgc tgaattcgct catggaatct ggggtggaga
     1921 gatcgcagga actcagggtg ttttaattgg acacagaaaa ggggagagaa atctgttatt
     1981 cttccgatgt acagatcctg aaaaattaac atttacagca gaccttttag atagtgatat
     2041 cggaccagca aatgtacttc attatacaaa tgatggtgaa gattatatct tatctgcaaa
     2101 cagagaaatt gatgaaatcg cattgtataa agtggaaaaa taactacaac tgcaggaaat
     2161 ctgagaatga taattaacag attgtgtact ggactgatat atagcaatgc ggtataataa
     2221 aaagtgcttt ggataatgaa tgggaggtgg aaaacaatga aggaatatga ttcacttagc
     2281 gataacgaaa agtgcaacat gcttgccagt gttgcnnnnn nnnnncctct attataatgc
     2341 ggaaatgact cagaatcaga ttgcggaacg tttttttacc tcacgttcta agatttcaag
     2401 aatgctaaaa gaggcaagac agcttggaat cgtagagatc aagattttag agccatggga
     2461 ccgcaatgta gagctggaac aggaatttat gagaagattt gcgttgaaag atgtgagagt
     2521 gatttctgtc aaagaagcaa ataatacgat ggtccttcag aaattgggtg aagttgcagc
     2581 atactatcta gataatctgt taaatgataa tatgattctt ggaatttcat gggggaatac
     2641 aatgtatcat actgtcaaag cggtcaagac aagtaagaat atcccgatca cagtcgtacc
     2701 gatcatggga gcagccaatg taaggactcc agagagagat tccttggatc tttcgaaaga
     2761 acttgcctat gcatatggtg gaacgtatca ctacatttat gctccgcttt ttgtgaattc
     2821 ggaagaagtc agggacagtc ttgaacaaga gccgaatatt aaaggatgtt tagagcttgc
     2881 aaggaatgca gatatcattc taaccagtgt tggttctatc gtatataaga gttggaaatc
     2941 ttatttaagt accagagatt tatataatct tgagaagaaa ggtgcgatcg gacatatcgg
     3001 aggacatttt tacgatatgg aaggaaatga agtcataaca ccgtttgtta ataagatgat
     3061 cgggcttgga attgaagata tcaagaatac agagaatgtg atctgtgtcg caggactgga
     3121 gatgaaggca gaagcgattc ttggagctgt gagaggcgga tatattaata cactgatcac
     3181 agatgaagag gcagcgaagg cagtgcttgc attatgtaaa taatatttat atcatattaa
     3241 taaaaaaaga ctgtaaaata agttagtcag acttatttta cagtcttttt tattacgatt
     3301 atttagcttt tacaccttta gaaccagacc agttagaata
//
LOCUS       DAOMUE010000226         3302 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8234_length_3302_cov_2.210040, whole genome shotgun sequence.
ACCESSION   DAOMUE010000226 DAOMUE010000000
VERSION     DAOMUE010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8234_length_3302_cov_2.210040"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..448)
                     /locus_tag="PLC71_08975"
     CDS             complement(104..448)
                     /locus_tag="PLC71_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02811.1"
                     /translation="MKISTVIIGIALFAIVTMILYGWGIVKQKNQTSDLMNLLFSKGQ
                     DKIKKYLKQHEYVTISQVEQICEGLEAKQPFSRNKAVVKDKRDYAKKLVEYMLKTNQL
                     EQEGSRYKMHKK"
     gene            complement(478..813)
                     /locus_tag="PLC71_08980"
     CDS             complement(478..813)
                     /locus_tag="PLC71_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRM02812.1"
                     /translation="MKEKKNYIDNIPKINDMKWDVSEDGIVEITVENTGFYNTIAQKI
                     FKKPRYSFIKLDEYGSFVWQKIDGKKSIYEIGKELQAVHEGAATQLYERLSQYFAILE
                     RNKYIVFEE"
     gene            complement(894..2831)
                     /locus_tag="PLC71_08985"
     CDS             complement(894..2831)
                     /locus_tag="PLC71_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HRM02813.1"
                     /translation="MEKKNDFKPFISADKVLPEFTVTSVILGMILAVIFGGANAYLGL
                     RVGMTVSASIPAAVISMGVIRVILKKDSILENNMVQTIGSAGESLAAGAIFTIPAIFI
                     WASEKGSGVTAPSFVSIALIALCGGILGVLFMVPLRTALIVEEHGVLPYPEGTACAEV
                     LLAGEEGGSKSKVVFAGLGIAAVYKFIADGLKLFPSEVEFSMQGQYTTSVGMDVLPAL
                     AGVGYICGVQVSSYLFAGGVLSYLVLIPAIAYFGGDSLSKVVDETGKALAFNQLGASQ
                     IWSNYVRYIGAGAVAAGGLISLIKTFPTMIKTFGHAMKGFGKKGDSQLRTQQDISMKV
                     VLGGILIIAVLIWLLPEIPVSFLGAVMIVVFGFFFATVSSRMVGIVGSSNNPVSGMAI
                     ATLIVTTFILKATGNTGAAGMVSAISIGTVICIVAAMAGDTSQDLKTGYIVGATPRLQ
                     QIGELIGAIVAAFAIGGVMYLLNAAWGFGSDQIPAPQATLMKMVVEGVMGGTLPWVLI
                     FMGVFIAIVVEILGIPVLAFSIGLYLPIYLSTPIMVGGLIRWFIDNKRKYDSDAARKD
                     GSDRGVLYAAGMIAGEGIVGILLAVLAVFGVADKINLSSVYGAAFQSVGNWVGLAAFV
                     VLLAILFYFTRNKKTIEVDAK"
     gene            complement(2994..>3302)
                     /locus_tag="PLC71_08990"
     CDS             complement(2994..>3302)
                     /locus_tag="PLC71_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02814.1"
                     /translation="RKRCYETVSRKNLGENCPEHHCATPDAEEGYRFSKAMKNPGFIS
                     ANDIKKIYLPMGFENFKIEGRGLGSALVLEFLLYYMVKPEYQICVREEVYLDNMLDLF
                     "
BASE COUNT          994 a          765 c          465 g         1078 t
ORIGIN      
        1 gctgcagatt tcttgatccg tgagggattg aacaaatctc ctcaaaatat ttaaatactt
       61 tgtttggctg taattctcct aatactgaca ttttatgtcc tccttatttt ttgtgcattt
      121 tgtaacgact gccctcttgt tcgagctgat tcgttttcag catatattct acaagtttct
      181 tcgcatagtc tcgtttatcc ttcacaaccg ctttatttct tgaaaatggc tgtttcgctt
      241 caagaccttc gcaaatctgt tctacctgtg aaattgtaac gtattcatgt tgttttaaat
      301 acttttttat tttatcctgt cctttactaa aaagcaaatt catcaggtca cttgtctgat
      361 tcttttgttt tacgattccc catccatata ggatcatcgt cactatggca aataaagcaa
      421 tgcctatgat cactgttgat attttcatat taaatccttt ttcactttta ataatattta
      481 ttcttcaaaa acaatgtatt tatttctttc taaaattgca aaatactgtg ataatctttc
      541 atataactgt gttgctgcac cttcatgaac tgcttgaagt tcttttccta tctcataaat
      601 acttttcttt ccatcaattt tctgccacac aaaacttcca tattcatcta atttgataaa
      661 actgtaacgt ggctttttaa atattttctg cgcgatcgta ttataaaatc cagtattttc
      721 tactgtaatt tctacaattc catcttctga cacatcccat ttcatatcat tgatcttcgg
      781 gatgttgtca atataattct tcttttcttt cataatacac ctttttattt agaaagcact
      841 actgcagaat cgcagcagtg ctttcttctc actgaaatac atatttattt ttattatttt
      901 gcgtctactt caatcgtctt tttatttctt gtgaagtaga ataatatagc taacaatact
      961 acaaaagctg ctaaacctac ccagttacct acactctgga aggcagctcc atatacagat
     1021 gacagattga tcttatctgc aacaccaaat actgccagta cagccagaag gataccaacg
     1081 ataccttctc ctgcaatcat acctgcagca taaaggactc ctcgatctga tccgtctttt
     1141 cttgccgcat cactgtcata ttttctctta ttgtcaataa accaacgaat cagtccacca
     1201 accatgatcg gtgtacttaa gtaaattggt aaataaagac cgattgaaaa tgctaacact
     1261 ggaattccaa ggatttcaac aacgattgcg ataaacacac ccatgaaaat caatacccat
     1321 ggtaatgtac cacccataac accttcaaca accatcttca tcaaagttgc ctgtggtgct
     1381 ggaatctgat cagaaccgaa tccccatgct gcgttcagaa ggtacataac tcctccgatc
     1441 gcaaatgctg ctacgatcgc accgatcaat tctccgatct gctgtaatct aggtgtcgca
     1501 cctacgatat aacctgtctt taagtcctgt gaagtatctc ctgccatagc tgctacgata
     1561 cagataacag taccaataga aattgctgat accatacctg ctgctcctgt attcccagtt
     1621 gcttttaaga taaatgttgt tacgatcagt gtcgcgatcg ccatacctga tactgggtta
     1681 ttactacttc ctacaatacc aaccattcta gatgataccg ttgcaaagaa gaaaccaaat
     1741 acaacaatca tcactgctcc caagaagctt actggaatct ctggtaataa ccagattaaa
     1801 actgcaatga tcaggattcc tccaagaacc actttcatag aaatatcctg ctgtgttctt
     1861 aactggctgt cacctttctt accgaatccc ttcatagcat gtccaaatgt cttgatcatt
     1921 gttgggaatg tcttgatcag actgatcaga ccaccagctg ctactgcacc ggcaccaata
     1981 tatctgacat agttactcca gatctggctt gcacctaact gattaaatgc aagtgctttt
     2041 cctgtctcat cgacaacttt tgataaagaa tctccgccaa aataagcaat tgcaggaatt
     2101 aatacaagat aagatagcac accacctgca aaaagatagc ttgatacctg cacaccacag
     2161 atataaccta cacctgctaa tgccggaaga acatccatac caacggatgt tgtatactgt
     2221 ccctgcattg aaaattcaac ttcactaggg aaaagtttta aaccatctgc aataaattta
     2281 taaactgccg caattccgag tcctgcaaat acaactttag atttacttcc tccttcttct
     2341 ccagctaaaa gcacttctgc acaagctgtt ccttcaggat aaggaagtac cccatgttct
     2401 tctacgatga gggctgtacg aagaggtacc ataaaaagta ctcctaaaat accaccgcaa
     2461 agcgcgatca atgcgataga tacaaaagat ggagcagtaa ctccacttcc cttttctgat
     2521 gcccaaataa aaatagcagg gattgtaaag attgctcctg ctgctaatga ttcacctgct
     2581 gaaccaattg tctgtaccat gttattttca aggatggaat ctttctttaa gatcacacgg
     2641 atcacaccca tggaaataac tgccgctgga atggaggctg atacagtcat accaactctt
     2701 agccccaaat aagcgtttgc accaccaaat ataaccgcca gtatcatacc aaggattact
     2761 gatgtaactg taaactctgg taatacttta tctgcggaaa taaaaggctt aaagtcattt
     2821 ttcttttcca tttttctctc ctattttcaa tatatatttc actgtatctc tctattatac
     2881 taaatcatac aagaaaaaga aaccccttct ttttcttaaa cttcttaact tagattcctc
     2941 gcatttttta ataaacagtt ttgctttatg tgttgtataa aatgcattat acattaaaat
     3001 aaatcaagca tattatccaa ataaacttct tctctaacgc atatttggta ttctggtttt
     3061 accatatagt atagtagaaa ctccaaaacc aacgcacttc ccaatccacg tccttcaatt
     3121 ttaaaattct caaaccccat tggaagataa attttcttta tatcattagc actgataaat
     3181 cccggattct tcatggcttt tgaaaaacga tatccttctt ctgcatccgg cgtagcacag
     3241 tgatgttccg gacagttctc tccaagattt ttacgactta ctgtttcata acaacgcttt
     3301 ct
//
LOCUS       DAOMUE010000227         3291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8271_length_3291_cov_2.858776, whole genome shotgun sequence.
ACCESSION   DAOMUE010000227 DAOMUE010000000
VERSION     DAOMUE010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8271_length_3291_cov_2.858776"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1327
                     /locus_tag="PLC71_08995"
     CDS             146..1327
                     /locus_tag="PLC71_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRM02815.1"
                     /translation="MTKDGYYTSGEFAKKANVTLRTIRYYDRQGILKPSKVADNGYRL
                     YTDADFAKLQKILSLKYLGFSLEEIMTMTINDEDQDFIKESIGLQLYLVQKKMEHLKL
                     VEQSLEEMEQKMDNEEEIDWTQMMNLLHVTNLEKGLVEQYKNASNLDIRIGLHQKYSQ
                     NPQPWFEWIYEQLNLKEKDEVLELGCGTGELWKNKNLPKNVHVTLSDQSQGMVKDARN
                     YVGEGKGQISYQMIDCRQIPKEDHSFDKVIANHVLFYLDDRQQVFNEIKRVLKQNGTF
                     ICSTYGSSHMQEITQLIKDFDERITLSDHKLYDVFGLENGEEQLKKVFSQVKEIDYED
                     ELLVDQPEPLISYILSCHGNQHEYLNHRYLEFKDFVRKKMAAKGVIKITKSAGIFICK
                     P"
     gene            1338..2183
                     /gene="rfbD"
                     /locus_tag="PLC71_09000"
     CDS             1338..2183
                     /gene="rfbD"
                     /locus_tag="PLC71_09000"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRM02816.1"
                     /translation="MRVFVTGVKGQLGYDVMNELEKQGLEGIGVDIDEMDITDADQVN
                     KVIKEAAPDAVIHCAAYTAVDAAEDNEEICRKVNAQGTENIAKVCEELDIKMMYISTD
                     YVFNGQGERPWEPDDKREPLNVYGQTKYEGELAIEEHVKKFFTVRIAWVFGVNGKNFI
                     KTMLNLGKTHDHLTVVNDQTGSPTYTYDLARLLVDMIQTDKYGRYHATNEGICTWYEF
                     ACEIFKQAGMNVSVAPVSSDEYPAKAKRPSNSRMDKSKLTANGFTPLPTWQDALSRYL
                     KEIEY"
     gene            2203..2757
                     /gene="rfbC"
                     /locus_tag="PLC71_09005"
     CDS             2203..2757
                     /gene="rfbC"
                     /locus_tag="PLC71_09005"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRM02817.1"
                     /translation="MGKITVTPCDIEGLYVIEPTVFKDERGYFVETYNQNDMKEAGLD
                     MVFVQDNQSMSTRGVLRGLHFQKQFPQGKLVRVVRGKVFDVAVDLRSDSKTYGKWFGV
                     ELSAENMKQFYIPEGFAHGFLVLSDEAEFCYKCTDFYHPGDEGGLAWNDPEIGVEWPL
                     EEGVDLIISEKDQKWKGLKDTFKF"
     gene            2788..>3291
                     /locus_tag="PLC71_09010"
     CDS             2788..>3291
                     /locus_tag="PLC71_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRM02818.1"
                     /translation="MKGIILAGGSGTRLYPLTMVTSKQLLPIYDKPMIYYPLSVLMNA
                     GIRDILIISTPSDTPRFESLLGDGHQFGVNLSYAVQPSPDGLAQAFIIGEDFIGDDSV
                     AMVLGDNIFHGHGLKKRLLRAANKKEGATVFGYYVDDPERFGIVEFDSDGKAISIEEK
                     PEHPKSNF"
BASE COUNT         1208 a          544 c          690 g          849 t
ORIGIN      
        1 tctggatctt ctattctttt atttcgtttt cctaagatac agaaagacta cagctattac
       61 accagcaaat taaaattcct cttgacactt cccttacgtc accctttatg ataacgtata
      121 gagagaaaac caaggaggaa tctaaatgac aaaagatgga tactatacat ccggtgaatt
      181 tgcaaaaaaa gccaatgtaa cactaagaac gatccgctac tatgaccgtc aaggcatctt
      241 aaagccaagt aaagttgcag acaatggata tcgtctttat acagatgccg attttgccaa
      301 attacaaaaa atcttgtcat taaaatacct cggattttct ttggaagaga tcatgacgat
      361 gacgatcaac gatgaagatc aggatttcat taaagaatct atcggcttac agttatacct
      421 tgtacaaaaa aagatggaac acttaaaact ggtagagcaa tccttagaag agatggaaca
      481 aaagatggat aatgaagaag aaatcgactg gacacagatg atgaatcttc ttcacgtaac
      541 caatctggaa aaaggattgg tagaacaata taagaatgca tcaaatcttg atatccgtat
      601 cggacttcat caaaaatatt cacaaaatcc acaaccatgg tttgaatgga tctatgagca
      661 gttaaactta aaagaaaaag acgaagtatt agaactagga tgtggaacag gggaactgtg
      721 gaagaataaa aatcttccaa aaaatgtcca tgttacttta tccgaccagt cacaaggtat
      781 ggtaaaagat gccaggaact atgtcggaga gggaaaagga cagatttctt atcagatgat
      841 cgactgtaga cagattccaa aagaagatca cagtttcgat aaagtgatcg caaaccatgt
      901 attattttat ctggatgacc gacaacaagt atttaacgaa atcaaaagag ttctaaaaca
      961 aaatggaaca tttatctgca gtacctatgg aagcagtcat atgcaggaaa ttacccagct
     1021 gatcaaagat tttgatgaaa ggatcacgct ttctgatcac aaattatatg atgtctttgg
     1081 tctggaaaat ggagaagaac aattaaaaaa agtattttca caagtaaaag aaattgacta
     1141 tgaggatgaa ttgttagtcg atcagccaga accactgatc tcttatattt tatcctgtca
     1201 cggaaatcag catgaatatt taaatcatcg ttatttagaa tttaaagact ttgtaagaaa
     1261 gaagatggca gccaaaggag tgatcaagat cacaaaatct gccgggattt ttatatgtaa
     1321 accataaagg aggagttatg agagtttttg tcacaggagt caaaggacag cttggttatg
     1381 acgtaatgaa cgaactagaa aaacaggggc ttgaaggaat tggtgtggat attgatgaaa
     1441 tggatatcac agatgccgat caggtaaata aagtgatcaa agaagcagca ccagatgcag
     1501 tgatccattg tgcagcatac acagccgttg atgctgcaga agataacgaa gaaatctgca
     1561 gaaaagttaa tgcacaggga acagaaaata tcgcaaaagt ctgtgaagaa ttagatatca
     1621 aaatgatgta tatcagcaca gactatgtat ttaacgggca gggtgagcga ccttgggaac
     1681 cagatgataa gagagaacct cttaatgtct atggacagac aaaatatgaa ggggaacttg
     1741 ctatcgaaga acatgtaaag aaatttttca cagtccgtat cgcctgggta tttggagtga
     1801 atggaaagaa ctttatcaaa acaatgttaa acttaggaaa gacacacgat catttaaccg
     1861 ttgtcaatga ccagacaggg tcaccaacat acacatatga tcttgcaaga ttgttagtgg
     1921 atatgatcca gacagacaaa tatggaagat atcatgcaac aaatgaagga atttgcacat
     1981 ggtatgaatt tgcctgtgaa atcttcaaac aggcagggat gaacgtatcc gttgcaccag
     2041 tcagcagtga cgaatatcct gcgaaggcaa aacgtccatc taacagcaga atggataaga
     2101 gcaagctgac agccaatgga tttacaccat taccaacatg gcaggatgca ttaagcagat
     2161 atttaaaaga gattgaatat taaaataaaa aggagaacga tcatgggaaa aattacagta
     2221 acaccttgcg atattgaagg actttatgtt attgaaccaa cagtatttaa agatgaaaga
     2281 ggatatttcg tagaaacata taaccagaac gacatgaaag aagcaggact tgatatggta
     2341 ttcgtacagg ataaccagtc tatgtctaca agaggcgtac ttcgtggatt acatttccag
     2401 aaacagtttc cacagggaaa attagtacga gttgtgcgag gaaaagtatt tgacgtagct
     2461 gtagacttaa gatctgattc caaaacttat ggaaaatggt tcggtgtaga attatcagca
     2521 gaaaatatga aacagttcta tattccggaa ggatttgcac atggcttctt agtattatct
     2581 gatgaagcag aattctgcta caaatgtaca gatttctatc atccaggaga cgaaggcggt
     2641 cttgcatgga atgatccaga aatcggagtt gaatggccat tagaagaagg cgttgacttg
     2701 atcatttctg aaaaagacca gaaatggaaa ggtttaaaag acacatttaa gttttaatta
     2761 ttacccagag ggagaaaggg aacacaaatg aagggaatta ttcttgccgg aggttccggt
     2821 accagactgt acccattaac catggtaaca tccaaacagt tactgccaat ttatgataaa
     2881 ccaatgatct attatccatt atctgtatta atgaatgcag gaatccgtga tattcttatc
     2941 atttccacac caagtgatac gccaagattt gagtctttgt taggagacgg acatcagttt
     3001 ggagtgaact tatcttatgc agtgcagcca tcaccagacg gacttgcaca ggcatttatc
     3061 attggagaag attttatcgg agatgacagt gtagctatgg tccttggaga taacattttc
     3121 cacggacatg gattgaagaa acgcctgtta cgtgccgcaa ataaaaaaga aggagcaact
     3181 gttttcggat actatgtaga tgatccagaa cgcttcggaa tcgttgaatt tgattctgat
     3241 ggaaaagcca tttccattga ggaaaaacca gaacatccaa aatccaattt t
//
LOCUS       DAOMUE010000228         3250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8388_length_3250_cov_2.624413, whole genome shotgun sequence.
ACCESSION   DAOMUE010000228 DAOMUE010000000
VERSION     DAOMUE010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8388_length_3250_cov_2.624413"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLC71_09015"
     CDS             complement(<1..251)
                     /locus_tag="PLC71_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HRM02819.1"
                     /translation="MEFEKQLEEWMKLDYVLPEDLPNIELYMDQVTTFLEEQLKNTKR
                     FEEDKIFTKTMINNYTKNHLLPPSNKKKYSRNHMILLIY"
     gene            394..2595
                     /gene="pcrA"
                     /locus_tag="PLC71_09020"
     CDS             394..2595
                     /gene="pcrA"
                     /locus_tag="PLC71_09020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HRM02820.1"
                     /translation="MGIYDTLNEQQQEAVFCTEGPLLLLAGAGSGKTRVLTHRIAYLM
                     DQGVNPYHIMAITFTNKAAKEMRERVDDLVGFGAEHIWVSTFHSTCVRILRRHIDKLG
                     YGNSFTIYDADDQKSLIKQICKQYKIDTKMMPERRIINEISSAKDEFMTPSEYETRHQ
                     YDFKKKKIAQIYKEYQKQLKANNALDFDDLIFKTVELFQFHPEVLDYYQERFRYIMVD
                     EYQDTNTIQFQLVSMLARKYQNLCVVGDDDQSIYKFRGANVKNILNFENVFPEAVTIK
                     LEQNYRSTKNILNAANEVIKHNKGRKTKKLWTENEEGDLIEFHQYGTEYEEARKIIHE
                     IEDLSKEGYDYKNMAILYRTNAQSRVFEESFMIKNIPYRIVGGTNFYQRKEVKDILSY
                     LKVVDNGLDDLAVRRIINVPRRGIGAATIEKINVYAVEHNISFLDACFSADSIDTLGN
                     AKKKINGFADLIREFRRKMQEGSLEELFKYITDETGYIANLKAEETEEAEGRIENINE
                     LLNKVVIYEQEAEEASLSELLEEIALVADIDNLEDSDNRVVLMTLHSAKGLEFPYVFI
                     CGMEDGIFPSYMTVMSEDDDDMEEERRLCYVGITRAKKKLYLSAAKRRMMQGRTQFNK
                     VSRFIDEIPEQLLQLDTGVNFKEKRPDKALFSSNRSNRFRKPYQAKSFTSTKMDTLPY
                     DVGDMVKHIKFGKGKVLEIVSGGRDYEVTVDFEKAGVKKMFASFAKLKKVE"
     gene            2662..2943
                     /locus_tag="PLC71_09025"
     CDS             2662..2943
                     /locus_tag="PLC71_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HRM02821.1"
                     /translation="MSGMEKLSDLLEMAKVSDIIATKKEKEDNKIVWALAIIGAIAAV
                     AGIAFAVYKYLTPDYMDDFDEDFDDDFDDDFFDEEDDTDVKEEDIEEVE"
BASE COUNT         1174 a          445 c          725 g          906 t
ORIGIN      
        1 atataaatta aaagaatcat gtgattcctg gaatatttct tcttattaga aggtggaaga
       61 agatggtttt tggtatagtt gttgatcatg gtttttgtaa atatcttatc ttcttcgaaa
      121 cgcttcgtat tctttagctg ttcttctaaa aaagtagtga cctgatccat atacaattcg
      181 atgtttggaa ggtcttctgg aagcacataa tcgagtttca tccattcttc gagttgtttt
      241 tcaaattcca taatgatttc tccttatctt atgactatat tatacggtta ttcaaaccat
      301 atgacaagag aaaaaacatc aggtgcaacg gactggactc gcttcgcttg ataaggtata
      361 atagcagtta agctttgaga aaggaattta tctatgggaa tttatgatac attaaatgaa
      421 cagcagcagg aagccgtttt ttgtacggag ggaccgcttt tgcttcttgc tggtgcaggg
      481 tctggaaaga ctcgtgtgtt gacgcacagg attgcttatc tgatggatca gggtgtgaat
      541 ccttatcata ttatggcgat cacatttaca aataaagcag cgaaggaaat gagagagagg
      601 gtcgatgatc tggtcggatt tggagcagag catatctggg tcagtacttt ccattcaact
      661 tgtgtgagaa tcttaagaag gcatattgat aagcttggtt atggaaacag tttcacgatc
      721 tatgatgcag atgatcagaa atccctgatc aaacagatct gcaagcagta taagattgat
      781 acaaagatga tgccagagag aaggatcatc aatgagattt cttctgccaa agatgaattt
      841 atgacacctt ccgagtacga aacaagacat cagtatgatt ttaagaagaa aaagatcgca
      901 cagatttata aagaatatca gaagcagtta aaggcaaata atgcactgga ttttgatgat
      961 ctgatcttta agaccgtgga attatttcag ttccatccag aagtgcttga ttattatcag
     1021 gagagatttc gttatatcat ggttgacgag tatcaggata caaatacgat ccagttccag
     1081 ttagtcagta tgcttgcaag aaaatatcag aatctctgcg ttgtaggtga cgatgaccag
     1141 tctatttaca aattcagagg tgcgaatgta aagaacatct taaactttga aaatgtattt
     1201 ccagaagcag ttacgatcaa actcgagcag aattatcgtt ccacgaagaa tatcttaaat
     1261 gcagcgaatg aggtgatcaa acacaataag ggaagaaaaa cgaagaaact ttggactgag
     1321 aacgaagaag gggatctgat cgaattccat cagtatggaa cagaatatga agaagcaaga
     1381 aagatcattc atgagattga agatctatcc aaagaaggtt atgattataa aaatatggcg
     1441 atcctttatc gtactaatgc gcagtctcgt gtatttgaag aaagttttat gatcaaaaat
     1501 attccatacc gtattgtggg aggcacaaac ttctatcaga gaaaagaagt gaaagatatt
     1561 ttgagttact taaaagttgt tgataatggg ctggatgatc tggcagtgcg aaggatcatc
     1621 aatgttccaa gacgagggat cggtgcagca acaatcgaga agatcaatgt gtatgcagtt
     1681 gagcataata ttagtttttt agatgcatgc ttttctgcgg atagcattga tacattggga
     1741 aatgcaaaga aaaagatcaa tggatttgct gatctgatcc gtgaattcag acgcaagatg
     1801 caggaagggt cgctggaaga attatttaag tatattacag atgaaacagg atatattgcg
     1861 aatctgaaag ctgaagaaac ggaagaagct gaaggaagaa ttgaaaatat caatgagctt
     1921 ttaaataaag ttgtaatata tgaacaggaa gcagaagaag cgagtctgtc agaattatta
     1981 gaagagatcg cattggttgc agatattgat aatctagaag attcagacaa cagggttgta
     2041 ttaatgacac tgcatagtgc aaaaggtctg gaatttccat acgtatttat ttgtggtatg
     2101 gaagatggaa tcttcccaag ttatatgacg gtgatgtctg aagatgatga cgatatggaa
     2161 gaagaaagac gtctttgcta tgtaggaatc acaagagcaa agaaaaagct ttatttaagt
     2221 gcggcaaaac gaagaatgat gcaaggaaga actcagttta ataaagtttc acggtttatc
     2281 gatgagattc cggaacagct attacagtta gacacagggg tcaacttcaa agaaaaacgt
     2341 ccagataaag cattgttttc atcaaatcgc agtaatcgat ttagaaaacc atatcaagca
     2401 aaatcattta cttcaacgaa aatggatact ttaccatatg atgttggaga tatggtgaaa
     2461 catataaaat ttggaaaagg aaaagtgctt gaaattgtct ctggaggccg tgattacgag
     2521 gtaacagtag actttgagaa agcaggagtg aaaaaaatgt tcgcatcctt tgcaaaacta
     2581 aaaaaagtcg aataaaatga acgaaaaagt tagtaaaata tagttagaag tgatataata
     2641 aagaaaacgc ataagaaagg aatgagcggc atggaaaaat taagtgatct tttagaaatg
     2701 gcaaaagttt cagacatcat tgcaacaaaa aaagaaaaag aagataacaa aatcgtttgg
     2761 gcgttagcga tcatcggagc gatcgcagca gttgcaggaa tcgcatttgc ggtatataaa
     2821 tatctgacac cagattatat ggatgatttt gatgaagact ttgatgatga ttttgacgat
     2881 gatttcttcg atgaagaaga tgatacggat gtgaaagaag aagacatcga ggaagtggaa
     2941 taagaaagct tcatatagga aactgaaacg aagaaagaac acctgttttt ggtgttcttt
     3001 ttttgataca attagcggct ttttcctatg atttttttag taagtctaag aaaaataaaa
     3061 aaagataatg aaaaaatata agatcagaaa ccgcataaac aggggatttg cgacaaagtg
     3121 ttcaagaaaa ataaaaaaag ttgaaaaaag ttgttgacat ttaaaggacg attctatata
     3181 ataaatcttg ctgttgatga aatagagaaa cagcaaagca cttacggggt gtggctcagc
     3241 ttggctagag
//
LOCUS       DAOMUE010000229         3249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8394_length_3249_cov_1.999374, whole genome shotgun sequence.
ACCESSION   DAOMUE010000229 DAOMUE010000000
VERSION     DAOMUE010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8394_length_3249_cov_1.999374"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..984)
                     /locus_tag="PLC71_09030"
     CDS             complement(241..984)
                     /locus_tag="PLC71_09030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRM02822.1"
                     /translation="MDNQNILAVVAGEEITQKDLDALIAALPKEQQAYAGNEHFRNQC
                     LEQIITVHLFAKLGEELKLEETEAFADNLAHAKREILAQMALGEAMKDITVSEEEAKE
                     YYKANENQFMAGETVHAKHILVDDEDKCQEILEKIIGEETTFEDAAKEFSTCPSKEKG
                     GDLGAFGRGQMVKEFEDAAFAAEVGHVVGPVKTQFGYHLIKVEDKKDAETSVYEDVAD
                     TIKNIILQQKRNDVYGNKIAELKEKYVEK"
     gene            1131..1952
                     /locus_tag="PLC71_09035"
     CDS             1131..1952
                     /locus_tag="PLC71_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRM02823.1"
                     /translation="MSVIQITDLNDPQLDIYARLSEGQLLHYYEPDLGIFIAESPKVI
                     QTAFEQGYEPISFLVEDRHIKTQAKDIILQYQDIPVYTASFDVLKQLTGFGLTRGMLC
                     AMRRKPLPALETICDHAKRIVILENVMNPTNVGAIFRSAAALNMDAILLSKGCSDPLY
                     RRSVRVSMGTVFQIPWTFLGDDTWPADGMHRLKELGYKNVAMALTDRSVSIDDEALMS
                     EDKLAIILGTEGDGLCQETIDACDYTVKIPMAHGVDSLNVAAASAVAFWQLAKRK"
     gene            1957..2070
                     /locus_tag="PLC71_09040"
     CDS             1957..2070
                     /locus_tag="PLC71_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM02824.1"
                     /translation="MNTNFEHYRIFYYVAKYGNLTKAATVLHTSQPSVTRI"
     gene            2103..2816
                     /locus_tag="PLC71_09045"
     CDS             2103..2816
                     /locus_tag="PLC71_09045"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HRM02825.1"
                     /translation="MKLTPEGEVFYEYIAAGCAQFFKAESNLSDMLSLENGTIYVSAT
                     ETALHCYLFQAMESFNEQYPNVHFKILNNSTRKSINIVKEGNVDFAVVSAPFQIEKPL
                     QQKVLRKYHDILIGGKRFEELKGQKISIKQLAQYPWISLTPEAITRKFLNQYFEKNGL
                     KFEADMELATTDMILPAVRHNLGIGFIPPEFAKDDLESGEVFEIEVKETFPQRNIILI
                     YDTEYPQSIAAKAFLRFLN"
BASE COUNT         1060 a          560 c          592 g         1037 t
ORIGIN      
        1 gatcttagaa gtcttgcaaa agatgatcct tttttcaatg aacttgctga tatttgcgac
       61 agacataatg gattatggag cgcagaagca gaaaaagcct tattaacaca ttacggtgta
      121 gaataacgta gtagatttag aagattgatc atgtaataaa aattaaacat tcaaaaagat
      181 gcctcccata aagtttttgt ggaaggcatc tttttatact ttacactatg taagaaaatt
      241 ctatttttct acatattttt cttttaattc agcgatctta tttccgtaaa catcgttacg
      301 tttctgctga agaatgatat tcttgatcgt atcagcaaca tcttcatata cagaagtttc
      361 tgcgtctttt ttatcttcaa ctttgatcag atgatatcca aactgtgtct ttactggacc
      421 tacaacgtgt ccaacttcag cagcaaatgc agcgtcttca aattctttca ccatctgtcc
      481 tcgtccaaat gcaccaaggt ctccaccttt ttctttggat gggcatgttg aaaattcctt
      541 agccgcatct tcaaatgtag tttcctcacc gatgatcttt tctaagattt cctgacattt
      601 gtcttcatca tctacaagaa tatgcttcgc atgaactgtt tctccagcca taaactgatt
      661 ttcattcgct ttataatatt cttttgcttc ttcttcagaa acagtaatat ctttcatagc
      721 ctctccgaga gccatctgtg caagaatctc tctctttgca tgagctaaat tgtcagcgaa
      781 agcttctgtt tcttctaatt ttaattcttc tcctaattta gcgaaaagat gaacagtaat
      841 gatctgttct aaacactggt ttctaaagtg ttcattccct gcatatgcct gttgttcttt
      901 tggtaatgca gcaatcagtg catctaaatc tttctgcgtg atttcttctc cggcaacaac
      961 agccaggatg ttctggttat ccatagtaat ttctcctttg attgaaatgt gtttgaaaat
     1021 ttattatatc acaaatcctt tatattttgt agatttagtt tgtgaaatac gatataataa
     1081 tgtggatttt aaagctacat ttcagaacaa agacatggag gtaagataag atgtctgtca
     1141 tacagatcac ggatctaaac gatccacaac tagatattta tgcaagattg tcagaagggc
     1201 agttattaca ttattatgaa ccagatcttg ggatttttat tgcggaaagc ccaaaggtta
     1261 tacagacagc ctttgaacaa ggatatgaac cgatttcttt tttggttgaa gatcgtcata
     1321 ttaaaacaca ggcaaaagat attatattgc aatatcagga tattcctgtc tatacagcat
     1381 cttttgatgt attaaaacaa ctaactggtt ttggactcac aagaggcatg ttgtgtgcca
     1441 tgcgtcgaaa accattacct gcgctcgaaa cgatctgtga tcatgcaaaa cgaatcgtta
     1501 ttctggaaaa tgttatgaat ccaaccaacg tcggagcaat ctttcgttct gctgcggcac
     1561 ttaatatgga tgccatactg cttagtaaag gctgcagcga tccattatac cgaagatctg
     1621 tacgcgttag tatgggaact gtttttcaga taccatggac atttcttggg gatgatacat
     1681 ggccagcgga tggaatgcat cgtttaaaag aacttggtta taaaaatgtt gcaatggcat
     1741 taactgaccg atctgtaagt attgatgacg aagctttgat gtcagaagat aaacttgcga
     1801 tcatccttgg aacagaaggc gatggtttat gtcaggaaac gattgatgca tgtgactata
     1861 cagtaaagat tccgatggca catggagtag attcattaaa tgtagctgct gcgagtgctg
     1921 ttgcattctg gcagcttgcg aaaaggaaat aaaacgatga atacaaattt tgaacattac
     1981 cggatttttt attatgtagc aaaatatgga aatcttacga aagctgctac agttttacac
     2041 acaagccagc catccgtaac aagaatttaa actgcagatt atttgaacga agcaaagttg
     2101 gtatgaagtt aacaccagaa ggtgaagttt tttatgaata catcgcagcc ggatgtgctc
     2161 agttttttaa agcagagagc aatttaagtg atatgctaag ccttgaaaat ggaacaatct
     2221 atgtaagtgc aacagaaacc gctctgcatt gctatttatt tcaggcaatg gaatccttta
     2281 atgaacaata tccgaatgtg cattttaaga ttttaaataa tagtacaaga aaatccatca
     2341 atatcgtaaa agaaggcaat gttgattttg cagttgtatc tgcaccattt caaatagaaa
     2401 aaccgttaca gcaaaaagtt ttaagaaaat accatgatat tttaattggt gggaaacgtt
     2461 ttgaggaatt aaaaggtcaa aaaatttcta tcaagcaact tgcccaatat ccttggatca
     2521 gtctgacccc agaagctatc acaagaaagt ttttaaatca gtattttgaa aagaatggat
     2581 taaaatttga ggcagatatg gaacttgcaa ctacagatat gatccttcca gctgtaagac
     2641 ataatcttgg aattggtttt atcccaccag aatttgctaa agatgatctg gaatctggag
     2701 aagtctttga gattgaggta aaagaaacgt tccctcagcg taatatcatc ttgatctatg
     2761 atacagaata tccacaaagt atcgcagcta aggcattctt aagattctta aattagggga
     2821 ttccatcgga aattcctatg atattaaaaa acctccaaag aacagcaccc gttgcttatc
     2881 tctggagatt tctcagaaca atatataaaa agtgtaaact agttacctga cttaatatgt
     2941 ttttatctta tcatgaataa aaacaagtgt aaaatacatt ttttacatca tatccatatc
     3001 aaaatgatat ggataaaatt ctgaagattt tcttaggaaa tgaaaaaaac aaagattaac
     3061 ggtttgatat caaaagaata tgtggtaaaa tacggacagg aaaataagga gggaacttta
     3121 gaatgtttac aaaaaaattt acagtcatca tcagtttttt tctgatcatt gcaattggcg
     3181 ttggtggtat gcttgggtat tatgatgcgc gactggaagc cgcagtgacg ccaaaaaaac
     3241 aatctatgg
//
LOCUS       DAOMUE010000230         3246 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8403_length_3246_cov_2.451583, whole genome shotgun sequence.
ACCESSION   DAOMUE010000230 DAOMUE010000000
VERSION     DAOMUE010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8403_length_3246_cov_2.451583"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PLC71_09050"
     CDS             complement(<1..530)
                     /locus_tag="PLC71_09050"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HRM02826.1"
                     /translation="MVVAIDSLKGSLTSIQAGEAIEKGIKKVDLEAEVVIKPLADGGE
                     GTVDALVDGMGGNMEFIKVTGPAGKSVLAKYGIIRPTKSDMEETSTAIMEMSQAAGIT
                     QVTDKERNPLYTTTYGVGQMIVDAIHKGCRRFIMGIGGSATNDGGVGMLQALGYDFLD
                     KDGNQIKHGAIGLKDLV"
     assembly_gap    533..542
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(632..>3246)
                     /locus_tag="PLC71_09055"
     CDS             complement(632..>3246)
                     /locus_tag="PLC71_09055"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HRM02827.1"
                     /translation="KFGSNEIPDSEPTTFWQEFKETFQDPMIKILLAIAVLMIVMFFF
                     GYAEIYEPMGTIIAVLIVAFVTAKTGVASDTKYRELKKSTKKDQCKVHRNGVVAVIDV
                     DDVVVGDQVLLQSGDKIPADGILHTGNLKVNNSALNGEAEECEKKAAGEGVYLAEEIT
                     GDTFVDSYSLFRGAVIFDGEGIMDVQKVGLHTMMGKMAEEMQEEEPDSPLKVKLSKLA
                     DQISKFGYIGAIVIAVMYFVYFIIMAGGFQAYFSMGPAKVVQDLIDAVSLAVVIIVCA
                     VPEGLPLMISLVLMQNTSKMLDHNVLVRKAEGIETAGSLNILFSDKTGTITKGELEVV
                     DFFTADGTSISANELRHHGKVKGLLDLAIGKNTQAMFDVYHKVIGGNATDQALLKFIG
                     EETFCMLDGNDGCKVSAHQGFNSSNKFSQARIESIGKTFYKGAPERLLAKATKYLDGD
                     GQIKEIDQKALNQKIDSLAAKAMRVLAFGYSEKELVKNQINDDLVIIGLVAIRDDVRP
                     SAKDAIRQVQEAGIQVVMITGDRLETAVAIAKDAGLLKNESDRALSSAQLNQMSDEEV
                     KAILPQIRVIARALPTDKSRMVRLCQEMNLVVGMTGDGVNDSPALKRADVGFAMGSGT
                     EAAKEAGKIVILDDNFSSIKDAIWYGRTIYHNILKFCKFQLVINVTAVVVSAVAPFLG
                     IEEPLKVTHLLFVNLVMDGLGAMMLGNEPALSKYMKEAPRRRDEGIISKDMMTQIGFM
                     GIWLVILSFLFLKLPVLTNLFDNKAQHLTAYFVLFIFSALFNGFNVRDERFGIFKRLN
                     ENPDFLKVFFIIMLVQIMIVNAAAIPFQVFIWIGKMFSCIPFGAKGWIVTVLLSMTMI
                     PVDCLRKFLFGCGK"
BASE COUNT          924 a          841 c          455 g         1016 t
ORIGIN      
        1 actaaatctt ttaatccgat cgcaccatgt ttaatctggt ttccatcttt atctaagaaa
       61 tcatatccta acgcctgcag cattccgaca ccaccatcat tggttgcact tccaccaatt
      121 cccatgataa agcgcctgca tcctttgtga atcgcatcaa cgatcatctg accgactccg
      181 taagttgttg tgtataatgg attacgttct ttatcggtta cctgtgtgat acctgctgcc
      241 tgtgacattt ccataatggc tgtgcttgtt tcttccatat ctgattttgt tggacggata
      301 attccatact ttgctaacac ggacttccct gccggacccg taactttaat aaattccatg
      361 ttaccaccca taccatctac cagggcatcg accgttcctt ctccgccatc tgcaagaggt
      421 tttatgacta cttctgcttc taaatcgact ttttttattc ctttttctat agcttctccc
      481 gcctgaattg atgttaagct tccttttagt gaatcaatcg ctaccactat ttnnnnnnnn
      541 nnatccaggt tgaggaaaat taatatcgct ccaataaact ctattaaaaa agtaatggtt
      601 aaaatcctct ttaccgtcat aaaaacctcg cctattttcc acaaccaaac agaaattttc
      661 tcagacaatc cacaggaatc atcgtcattg ataacaatac tgtaacaatc cagccttttg
      721 ctccaaatgg aatacaacta aacatctttc cgatccatat aaatacttga aatggaattg
      781 ccgctgcgtt tacgatcata atctgtacca gcatgataat aaaaaatact ttcagaaaat
      841 ccgggttctc atttaatctt ttgaaaatcc caaatcgttc atccctgaca ttaaatccgt
      901 taaataatgc actaaagata aataaaacaa aatatgctgt taaatgctgt gctttattgt
      961 caaatagatt cgtgagcact ggtaatttta agaataagaa acttaatata accagccata
     1021 tccccataaa tcctatctgc gtcatcatat ctttgctgat aataccctca tcacgtcttc
     1081 tcggtgcttc tttcatatat tttgaaagtg ctggttcatt tccaagcatc attgctccga
     1141 gcccgtccat cacaaggttt acaaataata aatgtgtgac ttttaatggt tcttctattc
     1201 ccaaaaatgg tgcaactgca ctgacaacaa ctgctgtcac attgatcact aactggaatt
     1261 tacagaactt caagatatta tgatagatcg ttcttccata ccagattgca tccttgattg
     1321 atgagaagtt atcatccaaa ataacaatct ttcctgcctc tttcgcagcc tctgttccac
     1381 ttcccattgc aaatccaaca tctgcacgtt tcaatgctgg ggagtcattc actccatcac
     1441 ctgtcatacc tacaacaaga ttcatttcct gacacaggcg caccattctt gacttatccg
     1501 ttggcaatgc tcttgcgatc acacggatct gcggtaaaat agctttgact tcctcatcac
     1561 tcatctgatt taactgtgca gaagataaag ctctatctga ttcatttttt aacaatcctg
     1621 catcttttgc gattgctact gccgtctcca ggcggtctcc tgtgatcata acaacctgaa
     1681 ttccagcttc ctgtacctgc ctgatggcat ctttggctga tggtctgaca tcgtctctga
     1741 tcgccactag tccaatgatc acaaggtcgt cgttgatctg attcttcaca agctcttttt
     1801 ctgaatatcc aaaagcaaga acacgcattg ctttcgctgc cagagaatca atcttttgat
     1861 ttaatgcttt ctgatcgatc tctttgatct gtccatcacc atctaaatat tttgttgctt
     1921 ttgcaagcaa tcgttcgggt gctcctttat aaaatgtctt gcctatgctc tcaattctcg
     1981 cctgactgaa cttattggaa gaattgaatc cttgatgggc actaacctta catccgtcat
     2041 tcccatccaa catacaaaat gtctcttcac caataaattt caacaacgcc tgatccgttg
     2101 catttccacc aatgacctta tgataaacat caaacatcgc ctgtgtattc ttcccaatcg
     2161 caagatctaa cagccctttc accttcccat gatgtcttaa ttcatttgca gaaattgaag
     2221 ttccatccgc tgtaaagaaa tctacaactt ccaattctcc ctttgtgatc gttcctgtct
     2281 tatcactgaa taaaatattt aaagatcctg ctgtctcaat tccttctgcc ttacgaacta
     2341 atacattatg atctaacatt ttacttgtat tttgcattaa tacaagagat atcataagcg
     2401 gcaatccttc tggtactgca catacaatga tcacaaccgc aagtgacact gcatcgatca
     2461 aatcctgcac aacctttgct ggtcccatag aaaaatatgc ctgaaatcca cctgccatta
     2521 taatgaaata tacaaaatac ataactgcga tcacgatcgc accgatatat ccaaacttcg
     2581 atatctgatc agcaagtttt gaaagtttta ccttcaatgg agaatctggt tcttcttcct
     2641 gcatctcctc agccatcttc cccatcatcg tatgaagtcc aaccttctga acatccatta
     2701 taccttcacc atcaaagatc acagctccac gaaacagaga atatgaatca acaaatgtat
     2761 ctcctgtaat ctcctccgca agataaacac cctctccggc tgctttcttc tcacactcct
     2821 ctgcttctcc atttaaagca gaattattga ctttcaggtt tcctgtatgc aaaataccat
     2881 ccgcaggaat cttatcccca gactgcagta aaacctgatc cccaacaacc acgtcatcaa
     2941 catcgatcac tgcaaccaca ccatttctgt gtaccttaca ctgatccttc ttcgtactct
     3001 tcttcaactc tcggtacttt gtatcactcg caacccctgt cttcgccgta acaaaagcaa
     3061 cgatcaacac cgcaatgatc gtccccatcg gttcataaat ctcagcatat ccaaagaaaa
     3121 acatcacgat catcaacacc gcgatcgcaa gcaagatctt gatcatcgga tcttgaaatg
     3181 tttccttaaa ttcctgccaa aacgtcgtag gttctgaatc tggaatctca ttggaaccaa
     3241 acttct
//
LOCUS       DAOMUE010000231         3235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8441_length_3235_cov_2.132075, whole genome shotgun sequence.
ACCESSION   DAOMUE010000231 DAOMUE010000000
VERSION     DAOMUE010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8441_length_3235_cov_2.132075"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="PLC71_09060"
     CDS             <1..1018
                     /locus_tag="PLC71_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02828.1"
                     /translation="QWKEVIIGAAGAVLMYLCWKSSGGIDYPANYLIILAMKDVDLKK
                     VMKAVFACGFVGLSYGFTWFFMNAPDKLTTVKDYGRGMIEVRYKFCTWHANTIHLMIM
                     VLIVSFLYAYYKKMKWWAFAIMFYFNYEFYQLSKSRTAFYCGSAAIIAYFILRYARKI
                     YEFKISLILLEIGNLVGIFLSIYYGLYSQLTDPTFMRLDQLITGRLTVARNCFLGAGI
                     PLFGSNIGGKVCGYGIYTQANDGYVTELGIVRTLLEYGPIVFGLFCAFMLIAVWVLYK
                     KGYFGAMVLLEIGFIACGVEAYFPAAYNLKAFVFGLAFYQLMGLFKGKEKEEKRGKAV
                     NASE"
     gene            1005..2627
                     /locus_tag="PLC71_09065"
     CDS             1005..2627
                     /locus_tag="PLC71_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HRM02829.1"
                     /translation="MHQNNRIISWIISFLFSICMVISISYDQRDNWSLIICDKKHMIS
                     ALIMTMILTMIVHMIMNVLYSKAGKSFIRLKTQANSGIGEKIFDHHPIAVPWSILIVL
                     WLPNYILYFPGCLTYDIIRQYEQFFSGNLTNHHPVLTTLFEGMFVKFGRNIGCQNVGI
                     AVYLLFTLLFTSGVFAICFYWMHKKNTKYWIRFTGLAFYGLFPIWSAYARTAVKDTLF
                     YPIFVLFVLFIFDIVLEPEKIFDSKVKSILFLIVSLLLCLVRHNGFYILIFTMPFCIV
                     GVKKYRRQLIVLFIIMVAFWEVYQKAILPMAGVKPGGKQEMLSIPFQQTARYVKYYGN
                     DVSTEEEKVIRKVLDYDTIGKNYDPDLSDPVKNTYKQKDEYLKDYFNIWFEMLKKHPT
                     AYIQATLNGTYGYWGYMTEIRYPYGYYVQPESMDIYQKEYKIYYSGKTKYIRDIYHGV
                     LDTIYQKTPLTLFTKPMIYLWILIGLLGMVCSFKKTIRYWIAFLPVVTSFLICLASPV
                     NGDMRYMLPITASTILYVAFVNEIIYKEYNEF"
     gene            2644..>3235
                     /locus_tag="PLC71_09070"
     CDS             2644..>3235
                     /locus_tag="PLC71_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRM02830.1"
                     /translation="MEKIAVLIPCYNEEKTIKKVVEDWKKELPEATIYVYNNNSTDRT
                     VEIAKEAGAIVRNEYQQGKGNVIRRMFREIDAQCYIMIDGDDTYPAQFGREMAEKVLE
                     YKVDMVVGDRLSSTYFEENKRPFHNLGNSMVRAAINHLFKSNIRDIMTGYRAFSYQFV
                     KTFPVLSRGFEIETEMSIHAVDKNMLVDNVIIEYRDR"
BASE COUNT         1095 a          393 c          677 g         1070 t
ORIGIN      
        1 tcaatggaaa gaagtgatta ttggagcagc aggagctgtt ttgatgtatt tatgttggaa
       61 aagctctgga ggaattgatt accccgcaaa ttatctgatc attctggcga tgaaagacgt
      121 agatcttaag aaagtgatga aagcagtatt tgcatgtgga tttgttgggt taagctatgg
      181 ttttacatgg ttctttatga atgcaccaga taaattaaca acagtaaaag attatgggcg
      241 aggaatgata gaagtacgat ataagttttg cacatggcat gcgaacacga ttcatcttat
      301 gatcatggtt ttgattgtta gttttttata tgcttattat aagaaaatga aatggtgggc
      361 atttgcgatc atgttctatt tcaactatga gttttatcaa ctatcaaaat caagaacagc
      421 attttattgt ggatcagcag cgatcattgc ctattttatt ttaagatatg caagaaaaat
      481 ttatgagttt aagatttcac tgattttatt agagatagga aatcttgtag gaattttctt
      541 atctatatat tatggacttt attctcaatt gacagatcca acatttatga gattagatca
      601 attgatcaca ggacgattga cagttgcacg gaactgcttt cttggagctg gaattcctct
      661 gtttggaagt aacattggtg gaaaggtttg tggatatgga atttatacac aggccaatga
      721 tggatacgta acagaattag gaattgtaag aacattgttg gaatatgggc caattgtgtt
      781 tggacttttt tgtgctttta tgcttatagc tgtatgggta ctttataaaa agggatattt
      841 tggggctatg gtgcttttag aaattggatt tatcgcatgc ggagtagaag catattttcc
      901 agccgcttac aatttaaagg catttgtatt cggcctggca ttttatcagt tgatgggatt
      961 atttaaggga aaagaaaaag aagagaaacg aggaaaggca gtcaatgcat cagaataatc
     1021 gaatcatttc atggattata tcatttttat tttcaatatg catggttata tctataagtt
     1081 atgatcagag agataactgg tcactgatca tatgcgataa aaaacatatg atcagtgcat
     1141 tgatcatgac gatgattctt acaatgatcg tacatatgat catgaatgta ttatattcaa
     1201 aagcggggaa gtcttttata aggttaaaga ctcaagcaaa cagtgggata ggagaaaaaa
     1261 tatttgatca tcatcctata gcagttcctt ggagcattct gattgtttta tggcttccaa
     1321 attatatttt gtattttccg ggttgtttaa cttatgatat tatacgacag tatgagcagt
     1381 ttttcagtgg aaatctgacg aatcatcatc ctgtattgac gacattgttt gaagggatgt
     1441 ttgtcaaatt tggaagaaac attggatgtc aaaatgtagg aattgcagtg tatctgttat
     1501 ttacattgtt atttacaagt ggtgttttcg caatatgttt ttattggatg cataagaaga
     1561 atacaaagta ttggattcgc tttacaggat tggcatttta tggattgttt cctatctggt
     1621 cggcatatgc aaggacagca gtaaaagaca cattatttta tccaatattt gttttatttg
     1681 tgttatttat atttgatata gtattagaac cagagaagat atttgattca aaagttaaga
     1741 gtattttgtt tttaatagtc tctttattat tatgtttggt cagacacaac gggttttata
     1801 tattaatttt tacgatgccg ttttgtattg taggtgtaaa aaaatatcga agacaattga
     1861 tagttttatt tataataatg gtggcgtttt gggaggttta ccaaaaagca attcttccca
     1921 tggcaggtgt gaagcctggc ggaaagcagg agatgctctc catccctttt caacagacag
     1981 cgagatatgt aaaatattat ggaaatgatg tttcgacgga agaggaaaaa gtgattcgaa
     2041 aagtattaga ttacgataca atcggaaaga attatgatcc agatctttct gacccagtaa
     2101 agaatactta caaacagaaa gacgaatatt taaaagatta ttttaacata tggtttgaaa
     2161 tgcttaagaa gcatccgacg gcttatattc aggcaacgtt aaatgggaca tatggatatt
     2221 ggggttatat gacagaaata agatatccat atggatatta tgtacagcca gaaagtatgg
     2281 atatatacca aaaagaatat aaaatatatt attcaggaaa aacaaaatat atcagagata
     2341 tatatcatgg ggttttagat acaatatatc agaaaactcc attgacatta tttacaaaac
     2401 cgatgatata tttatggatt ttgattggat tgcttggcat ggtatgttct tttaaaaaga
     2461 cgatcagata ttggattgca tttttgccag tagtgacatc attccttata tgtctggcat
     2521 cacctgtaaa tggcgatatg cgatatatgc ttccgattac agcgagtacg attttatatg
     2581 ttgcatttgt aaatgaaata atatataaag aatataatga attttaacaa aggagaatgc
     2641 gtaatggaaa aaattgcagt attgattccg tgttacaatg aagaaaagac aattaaaaaa
     2701 gtagtggagg attggaagaa agaacttcca gaggcaacaa tctatgtata taacaataat
     2761 tccacagatc gtacagtaga gattgcaaaa gaagcaggag ctattgtaag aaatgaatat
     2821 caacagggaa aaggaaatgt gatccgaagg atgtttcgag aaattgatgc gcaatgttat
     2881 atcatgattg atggagatga tacatatcct gcccaatttg gacgagaaat ggcagaaaaa
     2941 gttttagaat acaaggtgga tatggttgta ggggacagat tatcatctac ctattttgag
     3001 gaaaataaaa gaccttttca taatcttgga aatagcatgg tgagagctgc aattaatcat
     3061 ctttttaaaa gtaatatccg ggatattatg actggatata gagcttttag ctatcagttt
     3121 gttaaaacat ttccagtgtt atccagggga tttgaaatcg aaactgaaat gagcattcat
     3181 gcagtagata aaaatatgct ggttgataat gtgatcatcg aatatcgaga tagac
//
LOCUS       DAOMUE010000232         3215 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8509_length_3215_cov_2.335127, whole genome shotgun sequence.
ACCESSION   DAOMUE010000232 DAOMUE010000000
VERSION     DAOMUE010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8509_length_3215_cov_2.335127"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1417)
                     /locus_tag="PLC71_09075"
     CDS             complement(80..1417)
                     /locus_tag="PLC71_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HRM02831.1"
                     /translation="MKEHELRKRIKREKIQEQMPESTRKRMDDLLDGLPEETKRSKIH
                     RIWKNAVVFVAVLSLSTVTVFAGAKLITLGTGKLDTKHGNRQYQRSVKLNEIQKNNAK
                     AGISRTDQGITLRIDNVGLDEGNLLLYYTVSLNKTTTAEKLEKVKKDSLQERWNLNSI
                     WLGPQISIDGKANDEVNETGVTEAYRINSHKIKGVYRFNLTGKLKKKVKLDIKTNYLW
                     DTKGDWSMQLAVDRSKVAKKTKVIIRSKKSIVDRVIISPLGNTLRSNDKKHDLVIRDN
                     KGRYLYYEEKTNSEKSKDIYQFFKHTHTRSLEIIPVKKQSVVTKNGKVQKAVLNLKKN
                     EIVKVSDHTKLKVADVKKQKHNLRIYFKVLDYDGAILTDGLEGRFIVDNKGRSLIKDG
                     GSIDTWTDYEKEQLVLEFYNAFKGVDYTKAAKINFLKQKAVLNESQKQKIKIK"
     gene            complement(1404..1919)
                     /locus_tag="PLC71_09080"
     CDS             complement(1404..1919)
                     /locus_tag="PLC71_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRM02832.1"
                     /translation="MKKETFCRYIRQYETDMFRFAKSIVGTQADGEDAMQESILKAYE
                     NIDTLRSRRKFKAWIFQILANECYQILRNRKRQEPTDPFEFPEQEHSSDYWTEDMVSE
                     DGEILSYILRMPKQHQEVLILYYYDEFSTKEIAKILDIPQGTVKSRLARGRKQLKDLL
                     EMEGEGAYEGA"
     gene            complement(2122..>3215)
                     /gene="gatB"
                     /locus_tag="PLC71_09085"
     CDS             complement(2122..>3215)
                     /gene="gatB"
                     /locus_tag="PLC71_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRM02833.1"
                     /translation="DIRIHEIHMEEDAGKLVHDPWTGDSLVDFNRSGVPLIEIVSEPD
                     MRSADEVIAYLEKLRLIIQYLGASDCKLQEGSMRADVNLSVREVGAEEFGTRTEMKNL
                     NSFKAIKRAIEGEMERQIDLIEAGEKVVQETRRWDDTKGASYAMRSKEDAQDYRYFPD
                     PDLVPIEISDEWMDKVRDAQPEFRDEKKVRYKEEYDLPDYDIDIITGSKHLADIFEAT
                     IALGSEPKEVSNWLMGETIRLVNESEMDIDDVSFKPEHLAKLIEMIKNNEINRSVGKE
                     VFEKVFKEDIDPAAYVEEHGLKSMNDEGALKATIEEIVANNPKSVEDYKAGKKKAIGF
                     LVGQTMKATQGKANPGIVNKILTEILDNM"
BASE COUNT          753 a          698 c          464 g         1300 t
ORIGIN      
        1 ttcaagattt taccaataaa aataccagat ctctttttat tgaaatccgg tatttttgtt
       61 tatacaattg tatcgtatac tattttatct ttattttttg tttctgactc tcattgagaa
      121 ctgctttctg ttttaagaaa ttaatcttcg ctgctttcgt ataatcaacg cctttgaatg
      181 cattgtaaaa ttcaagtacc agctgttctt tttcataatc tgtccatgtg tcaatacttc
      241 caccatcttt gatcagggat cttcccttgt tatctacgat aaatctgccc tctagtccgt
      301 ctgttaagat tgctccgtca taatctagaa ctttaaagta aattctcaga ttgtgttttt
      361 gtttttttac atctgccact tttaatttgg tatgatcact gactttgaca atctcatttt
      421 tcttcagatt taatactgct ttctgtactt ttccattctt tgttaccaca gattgttttt
      481 taactggaat gatttccaat gatcttgtgt gtgtatgttt gaaaaactga tagatatctt
      541 tgcttttttc tgaattagtt ttttcttcat agtagagata tcttccttta ttatctctga
      601 tcacaagatc atgttttttg tcattgcttc gcaaggtatt tcctaatgga gatataataa
      661 ctctgtctac gattgatttc ttagatcgta tgatcacttt tgtcttcttt gcgaccttgc
      721 ttcgatcaac cgcaagctgc atagaccagt cacctttggt atcccataaa tagttggttt
      781 taatatccag ttttactttc ttttttaatt ttccagtaag attaaaacga taaactcctt
      841 taatcttatg gctgttgatc ctgtaagctt ccgttactcc cgtttcattt acttcatcat
      901 ttgcttttcc atcaatggaa atctgtggtc caagccatat actgttaagg ttccagcgct
      961 cttgtaaact atctttttta actttttcaa gcttttcggc tgtcgttgtt ttatttaatg
     1021 acactgtata atacaataat aaattacctt catccagacc gacattgtca attcttaatg
     1081 taattccctg atctgttctg cttattcctg cctttgcatt atttttttga atctcgttta
     1141 gtttcacaga cctttgatat tgcctgtttc catgttttgt atccaatttt cctgttccta
     1201 atgtgatcaa tttcgcacct gcaaacactg tcactgttga taaagataat acagccacaa
     1261 ataccacggc atttttccag attctatgaa ttttacttct ttttgtttct tccggcaatc
     1321 catctaacag atcatccatc ctttttctgg ttgattctgg catctgttcc tgtatttttt
     1381 ctctcttgat cctttttctt aactcatgct ccttcatatg caccttctcc ttccatctct
     1441 aataaatcct ttaattgttt tcttcctctg gcaagtcttg attttacggt tccttgcggt
     1501 atatctaaga ttttggcgat ttcttttgtg gaaaattcat cataataata taagatcaat
     1561 acttcctgat gctgttttgg cattcttaaa atataactta aaatttctcc atcctctgat
     1621 accatatctt ctgtccagta atcactggaa tgttcctgtt ctggaaattc aaacggatct
     1681 gttggttcct gtctcttcct gtttcttaag atctgataac attcatttgc caggatctga
     1741 aagatccatg ctttgaattt tcttcttgaa cgtaatgtat caatattttc ataagctttt
     1801 aagatgcttt cctgcatcgc atcttctcca tctgcctggg ttcccacgat actttttgca
     1861 aatcgaaaca tatctgtttc atactgtctg atataccgac aaaatgtctc tttcttcatt
     1921 cttacctcct ctcttttctt ttttccctct tttattgatt cacatattcg atggatttac
     1981 ttgccgtttg gttccctttt ttttattttt ttttaaagat tcataaatca gaaaaagaca
     2041 cttctatctt ctcttgataa aagtatcttt ttcactacta tgcaattctc aaggacttta
     2101 agacctcttg gaatgctatg gttacatatt atctaaaatc tcggttaaga tcttattaac
     2161 gattccaggg tttgcttttc cctgtgttgc tttcattgtc tgtcctacta agaatccgat
     2221 agctttcttc ttaccagctt tgtagtcttc gacagatttt ggattgtttg ctacgatctc
     2281 ttcgatcgtt gcttttaatg ctccttcatc attcatggat tttaatccat gttcttctac
     2341 gtatgctgca ggatcaatat cttctttgaa tactttctcg aagacttctt ttcctacgct
     2401 tcggttgatc tcattgttct taatcatctc gatcagtttc gcaagatgtt ctggcttaaa
     2461 tgatacatca tcgatatcca tctctgattc attgaccaga cggatcgtct ctcccattaa
     2521 ccagttagag acttcttttg gctctgatcc aagtgcgatc gttgcttcga agatatctgc
     2581 aaggtgtttt gatcctgtga ttatgtcgat atcataatct ggaaggtcgt attcttcttt
     2641 ataacggact ttcttctcat ctctgaactc tggctgggca tctcttactt tgtccatcca
     2701 ttcatcgctg atctcaatcg gtactaagtc tggatctggg aaataacgat aatcctgtgc
     2761 gtcttctttg gaacgcattg cataagatgc tcccttcgtg tcatcccaac gtcttgtttc
     2821 ctgtacgact ttctcacctg cttcgatcag gtcgatctga cgttccatct ctccttcaat
     2881 ggctctcttg atagctttga aagagtttaa gttcttcatc tctgtacgtg ttccaaattc
     2941 ttctgctcca acttcacgga cagataagtt cacatcagct ctcatggaac cttcctgtaa
     3001 tttacaatct gatgctccta agtactgaat gatcagacgt aacttctcaa gatatgcgat
     3061 aacctcatct gcagaacgca tatctggttc tgatacgatc tcgatcagtg gcactccaga
     3121 acggttaaag tctactaaag aatctcctgt ccatggatca tgcactaatt ttcctgcatc
     3181 ttcttccata tggatctcat ggattctgat atctt
//
LOCUS       DAOMUE010000233         3187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8610_length_3187_cov_2.427203, whole genome shotgun sequence.
ACCESSION   DAOMUE010000233 DAOMUE010000000
VERSION     DAOMUE010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8610_length_3187_cov_2.427203"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLC71_09090"
     CDS             <1..297
                     /locus_tag="PLC71_09090"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HRM02834.1"
                     /translation="IVDEMLEKIVADLPYENGDEVAVLVNGLGATPLDEQYIVTRRIN
                     QVLEEKGISVYKYYVGEYATAIEMAGFSISLLKLDDELKEYIDHPVDTPFFKQV"
     gene            311..931
                     /locus_tag="PLC71_09095"
     CDS             311..931
                     /locus_tag="PLC71_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRM02835.1"
                     /translation="METSYLIKLLKEISDIMAENKDKLIEMDGIVGDGDLGLTMSDGF
                     KAAYDAVSDGAIADAGKLLFAAGKAMANKVPSTMGSLMATGLKQAGKDLKGKETLEDA
                     DVVQIFASYEAGVAKLGEAKVGDKTFLDGLHPAVESLQASLAAGESLADMAGKAADAA
                     EEGFKATTTMLAVHGRAATRGEASRSLEDPGAKVASLIMQAFAKSL"
     gene            1014..1731
                     /locus_tag="PLC71_09100"
                     /pseudo
     CDS             1014..1731
                     /locus_tag="PLC71_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530664.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(1814..2530)
                     /locus_tag="PLC71_09105"
     CDS             complement(1814..2530)
                     /locus_tag="PLC71_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HRM02836.1"
                     /translation="MKIERISDNQIRCTLNKEDLEGKETLLSELAFGSEKAKGLFREL
                     MNKAATELGFETSEDNPLMVEAIPVSKECLILVITRVDNPEDFREHYKKLPKSLLAGQ
                     LAGADEDILSNLPDLLSDFGKSAFSADSGNDMKRVLADDGVGIFAFTSLDDVAFASKL
                     LNKYSLSSTLYKDSENQCYYLSLTDLDDSPNSTVMDEIHQLLSEYCKRLPNTYATKAL
                     FNEHLTTIIEKQAVEEMAAY"
     gene            2807..3001
                     /locus_tag="PLC71_09110"
     CDS             2807..3001
                     /locus_tag="PLC71_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02837.1"
                     /translation="MTSHDELSYYLSQGLAIRVDGITISDANLEHLNLILNEDDSYMK
                     EYVNDQEGEIYAIEYQKIKE"
     gene            complement(3047..3166)
                     /locus_tag="PLC71_09115"
     CDS             complement(3047..3166)
                     /locus_tag="PLC71_09115"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRM02838.1"
                     /translation="MECGFLSNPEELRSLQDNNYQKKAVQCILDGIESYLADI"
BASE COUNT         1061 a          526 c          693 g          907 t
ORIGIN      
        1 attgttgacg aaatgttaga gaagattgtt gcggaccttc catatgaaaa tggtgacgaa
       61 gttgcagtac ttgtaaatgg tcttggagca actccattag atgaacagta tattgtaaca
      121 cgtcgtatca atcaggttct tgaagaaaaa ggaatcagcg tatacaaata ctatgtggga
      181 gaatatgcaa cagcgatcga aatggctgga ttctcaatct cattattaaa acttgatgat
      241 gaattaaaag aatatatcga ccatccagtt gatacaccat tcttcaaaca ggtttaggag
      301 gcattttagt atggaaacat cttacttaat caaattatta aaagagatca gcgacattat
      361 ggctgagaat aaagataagc tgatcgagat ggacggaatc gttggtgatg gagacttagg
      421 tcttacaatg tccgatggat tcaaagcagc atacgatgca gtatctgacg gagcaatcgc
      481 tgatgcagga aaactgttat ttgcagcagg aaaagcaatg gcgaataaag ttccttcaac
      541 aatgggatct ttgatggcaa ctggtttaaa acaggctgga aaagatctga aaggaaaaga
      601 aacattagaa gatgctgatg tagtacagat ctttgcttct tacgaagcgg gagttgcgaa
      661 attaggtgaa gcgaaggttg gagataagac attccttgat ggacttcatc cagcagtaga
      721 aagcttacag gcttctttag cagcagggga atctttagca gatatggcag ggaaagccgc
      781 tgatgcagca gaagaaggat tcaaagctac tacaacaatg ttagcagttc atggacgtgc
      841 tgcaacaaga ggagaagcat ccagaagttt ggaagatcca ggagccaaag ttgcatcgct
      901 gatcatgcag gcatttgcaa aaagtttata attcttagca tgtgatttat aaagataaaa
      961 cagtacattg taatagagat attatagtgt actgtttttt taggaggtat gttatgttaa
     1021 attttcaata ttttacaccg accaaggttg tatttggcaa agatgcggaa ttgaaagttg
     1081 gtccgttggt aaaggaagaa gggtgtaaga aagttctggt acattatggt ggacaaagtg
     1141 ctaaaaagtc tggactgttg gacagagtct gcgaagcatt aaaggatttt gacattgatt
     1201 atgtaacact tggaggtgta gttccaaatc cacatctttc aaaagtatat gaaggatcct
     1261 gagaattatg aagcaagagc agagatcatg tggtgtggat ctctgtcaca taatggtttg
     1321 acaggctgcg gaatttgcgg aggtgactgg gcaacgcatc tgattgaaca tgagcttggt
     1381 ggaatgtttg atgtggctca tggagcaggg cttgcagctg tatggggaag ctgggcaaga
     1441 tatgttatgg acgagaaacc agaacgattt gcacagtttg cagtcgatgt tatgggagta
     1501 gaagaaagcg atgatgtaaa atcaacagca ttaaaaggta ttgaagcagt cgaagagttt
     1561 tatagaatga tcgagatgcc aacaaatatg aaagaacttg gaatcaatcc aaccgacgag
     1621 cagataaaag atatggcaat gaaagcaacg aatcatgaca cacagcagtt aggtgcattt
     1681 aaaaaattgt cagcaaagga cttagtagaa atttataaaa cagcaaaata aatccaataa
     1741 gaagccatgt atctataaaa tagaaaaagg aattcctgaa cacaaaatat tcggggattc
     1801 cttttcgttt gttttaataa gcagccattt cttcaacggc ctgtttttca ataatcgtcg
     1861 tcagatgttc attaaataaa gctttcgttg catatgtgtt tggcagacgt ttgcaatatt
     1921 ctgataaaag ctgatgaatc tcatccatga ccgtgctgtt cggcgaatcg tcaagatccg
     1981 tcaatgaaag atagtagcac tggttttcag aatctttata taaagtactg gacaggctgt
     2041 acttgttcag taactttgaa gcaaatgcaa cgtcatcgag agaagtaaaa gcaaagatgc
     2101 caactccgtc atcggcgagt acacgtttca tatcatttcc tgaatctgct gaaaatgcgg
     2161 attttccaaa gtctgaaagc agatctggaa gattagataa aatgtcttca tcagctcccg
     2221 cgagctgtcc agccaataga cttttcggca gttttttata atgttcacgg aaatcctccg
     2281 ggttatccac tcttgtgatc acaaggatca gacattcttt ggatacagga attgcctcaa
     2341 ccatcaaagg attatcttca cttgtttcaa atccaagctc tgtggctgcc ttattcatca
     2401 attcccggaa cagacccttt gccttctcag aaccaaaagc cagttcactt aacagagttt
     2461 cctttccttc taagtcttct ttatttaaag tacatcggat ctgattatca ctgatacgtt
     2521 ctattttcat aatcatcact cctttactac tttgatgtta ttatagtatt acttttgcgg
     2581 tgtgtaaagc tttttcaaga aatttacaat tttatccata aatagcttga aataatttcc
     2641 aattatgtat ataattacat tatgtcgcga caagacttac atttggaaac agtgcggaat
     2701 ttttatatac cacaagaaac actgaccatg taaggaagaa actttcattg tatatggtag
     2761 gaaattgtaa caagaaacat ctgcttaata agattggagg gtacgtatga cgagtcatga
     2821 tgaattatct tattatctgt cacaaggact ggcgatccgt gtggacggca ttacaataag
     2881 tgatgcgaat ctggaacatc taaatctaat actcaacgaa gatgattctt acatgaaaga
     2941 gtatgtcaac gatcaggaag gagagatcta cgccatagaa tatcagaaaa tcaaagaata
     3001 atatgcaaag agtatagcag agctgggaga ttaatttccc agctcattat atatctgcta
     3061 gatagctttc aatgccatct aagatacatt gaacagcttt tttctgatag ttattatcct
     3121 gaagtgacct taattcctca ggattagata aaaaaccaca ttccacgatc acgatcggat
     3181 aaggatt
//
LOCUS       DAOMUE010000234         3186 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8619_length_3186_cov_2.162887, whole genome shotgun sequence.
ACCESSION   DAOMUE010000234 DAOMUE010000000
VERSION     DAOMUE010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8619_length_3186_cov_2.162887"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PLC71_09120"
     CDS             complement(<1..807)
                     /locus_tag="PLC71_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HRM02839.1"
                     /translation="MKSNYKNIFTPLTIKNMTMRNRIMMTPMGTNYGEQTGEMSFLHI
                     DYYTERAKGGVGLIMVENASVDSPLGSNGTTQIRIDHDNYMPRFFKLCETLHAHGACV
                     GVQLNHAGASAQSKRTNMQPVSASDIPSKAGGEIPRPLKVEEIYEIVKKYGEAAARAQ
                     ACGFDCVEIHAGHSYLLSQFMSPTTNKRTDEFGGCPENRARFTKLVVEEVRKQVGPMF
                     PIFVRISADELMEGGNTLEDTLELLSYFQEEVDAFDVSAGLNGSLQFQIDA"
     assembly_gap    1041..1050
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1051..1906)
                     /locus_tag="PLC71_09125"
     CDS             complement(<1051..1906)
                     /locus_tag="PLC71_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRM02840.1"
                     /translation="MNLNQLYYFQKVAQLQHYHQTAKELNISQPSLSRSIANLEEELG
                     VSLFQKNGRNIELTKYGSIFLEHVNRIIDEIKIAENKMKSLAGSSSGHIDIGYVFPLA
                     KSYIPRLVRSFLNQQENHEITFSLSQEITKNLIADLKNEKYDVVFGSLVADEPEIEFV
                     PVVNQEMVIITPPEHPLKYKKKLVLEDLLDYPVIGYDKTSGLGRFTNSIYKQNHIKPS
                     IAFESSDENAIASLVAEDFGIGFVAHVESLQAYDVEILHLSNVKPYHTVYMAYLKNMQ
                     AVSIPEALI"
     gene            2104..2979
                     /locus_tag="PLC71_09130"
     CDS             2104..2979
                     /locus_tag="PLC71_09130"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRM02841.1"
                     /translation="MAERITGHTELIGLMAYPIRHSSSPAMHNEAFATLGLDYAYLAF
                     EVDNSTLEDAVKGLRALKMVGSNVSMPNKTVVGQYLDELSPAAKMAGAVNTIVNDNGK
                     LIGHITDGIGYMQSLKDNDIDVIGKKMTIAGAGGAATAIEIQAALDGVKEMSIFNIKA
                     KFWANAEETVKKIRENTDCIVNLYDLDDKDKLREEIADSYLFAQATGVGMKPLEGQTV
                     VPDETYFRPDLIVTDTVYAPRETETLRLAKKAGCKTMNGLGMMLFQGDAAFHLWTGKH
                     MPIDHMKEVLDIKYD"
     gene            3021..>3186
                     /locus_tag="PLC71_09135"
     CDS             3021..>3186
                     /locus_tag="PLC71_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRM02842.1"
                     /translation="MDKKYIPSALVLYLNYFIHGIGCSILSQQVVKEMLASQWGLKDV
                     MAVTSIAAALG"
BASE COUNT         1069 a          565 c          605 g          937 t
ORIGIN      
        1 ggcatcgatc tggaactgaa gagaaccatt taatccagca gatacgtcaa atgcatcaac
       61 ttcttcctgg aagtaagaaa gtaactctaa agtatcttct aatgtgtttc caccttccat
      121 cagttcatca gcactgatac gaacgaagat agggaacata ggtccaacct gtttacgaac
      181 ttcttcaaca actaattttg tgaagcgggc acggttttct ggacatccac cgaattcatc
      241 tgtacgtttg tttgttgtag gagacataaa ctgacttaat aagtaagaat gtcctgcatg
      301 gatctcaacg cagtcaaatc cacaagcctg tgcacgagca gcagcttctc catatttttt
      361 aacgatttca taaatttctt ctactttcag aggacgagga atctctccac ctgctttaga
      421 aggaatatca gatgcagata ctggctgcat gtttgttctc ttagactgag cagaagcacc
      481 agcgtggttt aactgaacac caacgcaagc tccgtgagcg tgtaatgttt cacataattt
      541 gaagaaacga ggcatatagt tgtcatgatc gatacggatc tgagttgttc cgttagatcc
      601 aagtggtgaa tcaacacttg cgttttcaac catgatcaga ccaactccac cttttgctct
      661 ttctgtatag taatcaatgt gtaagaaact catttctcct gtctgttcac cgtagttagt
      721 tcccattggt gtcatcatga tgcggtttct cattgtcatg ttcttaattg ttaatggtgt
      781 gaaaatgttt ttatagttgc ttttcattag aattgaacct ccaaaaaatt aaatccaaaa
      841 aatcaaaaaa ttaaattcca tataatccga ggttgttaga atcataaaaa ttgggtgaat
      901 gatttatgat ggctttagta taacatacgg ttaattattt aacaaataaa tcttttttga
      961 agaatcaata aaaaatatct attgtttctt ttgtgaattc aataaattta cgaacagcag
     1021 ggatcatcgg catattttta nnnnnnnnnn gtatcagtgc ctctggaata gaaacagcct
     1081 gcatattttt aagataagcc atataaacag tgtggtaagg ttttacatta ctaagatgta
     1141 ggatttccac atcatatgcc tgtaatgatt ctacatgtgc aacaaaacca atcccaaaat
     1201 cttctgcaac aagggaagcg atggcatttt catccgaaga ttcaaaagcg atagacggtt
     1261 tgatatggtt ttgtttgtaa atagaattag taaaacgtcc aagaccagaa gtcttatcat
     1321 aaccaatgac tggataatcc agcagatcct ctaaaacgag tttcttctta tattttaaag
     1381 gatgctctgg cggggtaatg atcaccattt cctgattaac aaccggaaca aactcaattt
     1441 ccggttcatc ggcaacgaga gaaccaaaaa cgacatcata tttttcattt ttcagatccg
     1501 caatcagatt ttttgtgatc tcctgactta gagaaaacgt gatctcatga ttttcctgct
     1561 gatttaagaa acttcgtaca agtcttggaa tataactttt ggcaagtgga aatacatatc
     1621 cgatatcaat gtgcccgctg gaactgccag ctaaggattt cattttattt tcagcaattt
     1681 tgatttcatc tataatacga ttgacatgtt ccaaaaagat agagccgtat ttggtaagtt
     1741 cgatattgcg tccgtttttc tgaaataaag aaacacctaa ttcttcttca agattagcga
     1801 tcgaacggct taaactaggt tgagagatat ttaattcttt ggctgtctgg tgataatgtt
     1861 gtaattgtgc aactttttga aaatagtaca gttgatttaa attcatacat accttcctta
     1921 ttataataat taatggttaa gttcattata acacgattga tagcctgaag ctatcaaaaa
     1981 acactaaaag atatattgga agtatcaatc aatatggaat ataatatagc cataaggttg
     2041 ttaaaaaatt aacttttggg tgaatgaaac taataacaag catattattt aaggagtaaa
     2101 attatggcag agagaatcac aggacatact gaattaatcg gattaatggc ttatccaatc
     2161 agacattcaa gctcacctgc aatgcataat gaagcattcg caacattagg acttgactat
     2221 gcatacttag cattcgaagt tgacaacagc acattagagg atgcagtcaa aggacttcgt
     2281 gctttaaaga tggttggatc taacgtatca atgccgaaca aaacagttgt aggacagtac
     2341 ttagacgagt tatctccagc agctaaaatg gcaggagctg ttaacacgat cgtaaatgac
     2401 aacggaaaac tgatcggaca tatcacagac ggtatcggat acatgcagtc attaaaagac
     2461 aacgatattg atgtgatcgg taaaaaaatg acaatcgctg gtgctggtgg agctgcgaca
     2521 gcaatcgaga ttcaggctgc attagacggt gtcaaagaaa tgtcaatctt caacatcaaa
     2581 gctaaattct gggctaatgc tgaagaaaca gtaaagaaga tccgtgaaaa cacagattgt
     2641 atcgtaaatc tttacgatct tgatgacaaa gataaattaa gagaagaaat cgcagacagc
     2701 tacttattcg cacaggcaac tggtgtagga atgaaaccat tagaaggaca gacagttgtt
     2761 cctgatgaaa catacttccg tccagacctg atcgtaacag atacagttta cgctccaaga
     2821 gagacagaaa cattaagact tgctaagaaa gctggatgta agacaatgaa cggtcttgga
     2881 atgatgttat tccagggaga tgctgctttc cacttatgga caggaaaaca tatgccgatc
     2941 gaccatatga aagaagtatt agatatcaaa tatgactagg atcagtcata accatatagc
     3001 aaacgagagg aattattaaa atggataaaa aatacatacc aagtgcctta gttctttatc
     3061 tgaactattt cattcatgga atcggatgtt ccatcttaag tcagcaggta gtaaaagaaa
     3121 tgctcgcaag ccagtgggga ttaaaagatg ttatggcagt aacttccatt gcagcagcat
     3181 taggat
//
LOCUS       DAOMUE010000235         3174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8660_length_3174_cov_2.352036, whole genome shotgun sequence.
ACCESSION   DAOMUE010000235 DAOMUE010000000
VERSION     DAOMUE010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8660_length_3174_cov_2.352036"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..294)
                     /locus_tag="PLC71_09140"
     CDS             complement(145..294)
                     /locus_tag="PLC71_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM02843.1"
                     /translation="MNVAANLLMEDSKVEIQEIALKMGYENPGKFSSAFKSVMGVTPA
                     KYRKH"
     gene            complement(298..927)
                     /locus_tag="PLC71_09145"
     CDS             complement(298..927)
                     /locus_tag="PLC71_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02844.1"
                     /translation="MGEFLFLNLDIAQFGSNTKTIIKDENSMVMMISDETGEGKMTVH
                     QILPGIMICFTDMHMEQCMSEFELNSGQKVLCIDQCREGRIEMENQPGIFSIMRENEL
                     RVDDRKHHKGMAYYPLRHYHGVSIFLEVHAAQKSLDEMFHGFSIDLKPYYDKTQMEKI
                     RAIHEQITSNLQVRVTIEELSKQYDMPATSMKKCFREIYGDSIYSYQKR"
     gene            927..1250
                     /locus_tag="PLC71_09150"
     CDS             927..1250
                     /locus_tag="PLC71_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02845.1"
                     /translation="MTDYQQRGETFSVLGSKYDLFEGIYGPADWKRLCQFLELDRTPI
                     CCAIPKNHPLCDHKQISMHDLDNQHIVTIPLNTDLTLPYGLMYANEPSEALKKFLRIV
                     KKLTW"
     gene            complement(1289..2626)
                     /locus_tag="PLC71_09155"
     CDS             complement(1289..2626)
                     /locus_tag="PLC71_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM02846.1"
                     /translation="MENYLFEKMSVPKAYMKLALPVVLSMIVSLVYNMVDTYFIALTG
                     VQELVAGVSLVAPMFTLMIAFGDIFGLGGSSAISRLLGEKKDNEAKKTCAFCIWISLV
                     FGLCISAILLLFRTQILGLLGVGKDTYQYANAYYTWIAIGAVSIIFSMVPSNILRTEG
                     LAVQSMAGSIIGSIVNIIFDPIFIFGLNQGAAGAAMATVLGNIIADIYYVYAVMKKSK
                     RLTCSPSHMKVTGRRIRDILMIGIPASITNIMQTFMMVMTNNFLLTYGTDKVAAMGIA
                     LKVNMITALVLVGFAFGGQPLVGYNYGAKNEKRLKNILKFAYLFEMGLGLLFTILMCI
                     FAPQIIKVFMDKPDIITNGAMMLRFQQIGMTFMSVSLISTCVCQAVGNAGGAFVLSIS
                     RQGVIYVLALFIMSNVFGYTGVLVSQACSDVVTALIAAVIMLKIMKKLTNKNE"
     gene            complement(2797..>3174)
                     /locus_tag="PLC71_09160"
     CDS             complement(2797..>3174)
                     /locus_tag="PLC71_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HRM02847.1"
                     /translation="SIYTPGTDQVDVKQARYGIDAVIRFLAYHGLIHMKVNGGYRSRI
                     VEENELVTVRTKTSGILVLKVKCGDHLSVGDEIAEIIDTYEGDVIEVIKSPCEGCLFY
                     HGSNPLIYSNTAIAKIIKDTDFI"
BASE COUNT          920 a          669 c          528 g         1057 t
ORIGIN      
        1 tctctttatt tatgttatac ttacctcgca caacaaacgt acgttcgata aagtgggctg
       61 cggctatggc taaattaaat aaaatcaaac cagatactat attaaaaaac ttctggaaaa
      121 ataatgaccg atttgctgat ctttttaatg ctttctatat ttggctggtg tcacgcccat
      181 cacgctttta aatgcggatg aaaattttcc tggattttcg tatcccattt tcaatgcaat
      241 ttcttgaatc tctactttgg aatcttccat taacagattg gctgcaacat tcatacgtta
      301 tctcttctgg taagaataaa tggaatctcc ataaatttca cgaaaacatt ttttcatgct
      361 tgttgctggc atatcgtatt gttttgacaa ttcttcgatt gtgactctca cttgtaaatt
      421 tgatgtaatc tgctcatgga tcgcacggat cttttccatc tgtgttttgt cgtaatatgg
      481 cttgagatca atggaaaaac catgaaacat ctcatctaaa gatttttgtg ctgcatgaac
      541 ttctaggaaa atgcttacgc catgataatg tcttaatgga taatatgcca tgcctttatg
      601 atgcttgcgg tcatcgaccc gcagttcatt ttcccgcata atactgaaga ttcccggctg
      661 gttttccatc tcgatgcgtc cttctctgca ttggtcgatg catagaactt tctgtccgct
      721 gtttaattca aactcggaca tacattgttc catatgcata tcggtaaaac agatcatgat
      781 acctggcaga atctgatgaa ctgtcatctt accttctcca gtttcatcgg aaatcatcat
      841 gaccatgctg ttttcatctt ttatgatcgt ttttgtgttg cttccaaatt gggcaatatc
      901 cagatttaaa aatagaaatt ctcccaatga cggactacca gcaaagagga gaaacatttt
      961 ccgttcttgg aagcaaatat gatctgtttg aaggtattta tgggcctgct gactggaaga
     1021 gactttgtca gtttcttgaa cttgacagga caccaatttg ttgtgccatt cctaagaacc
     1081 accctctttg tgatcacaaa caaatttcta tgcatgatct tgataatcaa catattgtta
     1141 cgattcctct gaatacagat ctgactctgc cttatggatt aatgtatgca aatgagccaa
     1201 gtgaagcttt gaaaaagttt cttagaattg taaaaaaact gacatggtaa aactgtcagc
     1261 ttttttgatt tcttcttgca acgtatattt attcattttt atttgtaagt tttttcatga
     1321 tcttaagcat aataactgct gcgatcaatg ctgtaaccac gtctgaacac gcctgtgata
     1381 ccaaaactcc tgtatatcca aacacatttg acatgataaa aagtgctaat acatagatca
     1441 caccctgtct gctaatggat aaaacaaaag ctcctcctgc atttccaacg gcctgacata
     1501 cgcaggttga gatcaggctc acggacataa aggtcattcc gatctgctgg aatcttaaca
     1561 tcatggctcc atttgttatg atatctggtt tatccataaa tactttgatg atctgtggtg
     1621 cgaaaataca catgagaatc gtaaataata gtcctaatcc catttcaaat aaatatgcaa
     1681 atttcaggat attttttaat cttttttcat ttttagcccc gtagttgtat ccgaccagcg
     1741 gctgtcctcc gaaggcaaat cctacgagta ctaaggctgt gatcatatta acctttaatg
     1801 cgattcccat cgctgcgact ttatctgttc catatgtaag taaaaaatta ttggtcatga
     1861 ccatcataaa ggtctgcatg atatttgtga tcgatgctgg aattccgatc attaagatat
     1921 ctctgatcct tcttcctgtg accttcatat gggatggaga acatgtcagt ctctttgatt
     1981 tcttcatcac cgcatataca taataaatat ctgcaatgat atttccaaga actgttgcca
     2041 tggcggctcc tgctgccccc tggtttaatc caaagataaa gattgggtca aagatgatat
     2101 ttacaatgga accaataatt gatcctgcca ttgactggac tgctaatcct tcggttctta
     2161 ggatattgct tggcaccatg ctgaaaatga tagatactgc accaatcgca atccatgtgt
     2221 agtaggcatt ggcatactgg tatgtatctt tgccaactcc taataatccg agaatctgtg
     2281 tacggaataa taataggatt gcactgatac atagtccaaa taccaaagat atccagatac
     2341 agaatgcgca tgttttcttt gcctcgttat ctttcttctc tcctaacaat cttgaaatcg
     2401 cggaacttcc tccaagtccg aagatatctc caaatgcgat cattaaggta aacatcggtg
     2461 ctacgaggga cactccggcg accagttcct gcactcctgt cagggcgata aaataggtat
     2521 ctaccatgtt atatactaat gatacgatca tacttaatac cactggcagt gcaagtttca
     2581 tatatgcctt tggaactgac attttttcaa ataaatagtt ttccataaaa actcctttcc
     2641 aaatcttatt ttataggcca aaaaacatcc ataatcctta cttaaaatgg attatggatg
     2701 tccaaaccgt agcattgcat acgcaatgcc ctatcatatt ttactttatc actaagtggc
     2761 attttaacag agtttatttt atacgtcaag attcatttaa ataaaatctg tatcctttat
     2821 gatctttgca attgcagtat tcgagtagat caatggattg gatccatgat aaaacaaaca
     2881 tccctcgcat ggcgacttga tcacttcgat cacatctcct tcataggtat ctatgatctc
     2941 tgcgatctca tctcctacac ttaaatgatc tccacatttt actttcaaga caagaattcc
     3001 tgaagtcttt gttcttactg ttacaagttc attttcttct acgatccttg aacggtatcc
     3061 cccattcacc ttcatatgga tcagtccatg atatgcaaga aatcggatca ctgcatcaat
     3121 tccgtaacgt gcctgtttta catcaacctg atctgttccc ggtgtataaa tcga
//
LOCUS       DAOMUE010000236         3166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8697_length_3166_cov_2.443266, whole genome shotgun sequence.
ACCESSION   DAOMUE010000236 DAOMUE010000000
VERSION     DAOMUE010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8697_length_3166_cov_2.443266"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..551
                     /locus_tag="PLC71_09165"
     CDS             192..551
                     /locus_tag="PLC71_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HRM02848.1"
                     /translation="MKFLRQFMIILLLSFLGEVLKMFIPLPIPASVYGLVLMLVCLMT
                     GILKTSQVKEAAFFLIEIMPVMFIPAAAGLIDSWKVLQPLLLPILVITVVITIFVMVV
                     TGKIAQMIAQKRGIKNE"
     gene            544..1236
                     /locus_tag="PLC71_09170"
     CDS             544..1236
                     /locus_tag="PLC71_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HRM02849.1"
                     /translation="MNEMFRQSLFAGVTISLIAYEIGLFLKRKLGLAILNPLLVSIVI
                     VILALKVFGISYEVYNASAKYISYLLTPATVCLAVPLYEQLELLKKNKVAVVCGILSG
                     TLASLTGIFVLCMLFGFNHQQYVTMLPKSITTAIGIGISEELGGIVTITVAVIIITGI
                     LGNVIADTVFHIFKIKNPLAKGLALGTASHAIGTARAIEMGPVEGAMSSLAVAVAGLL
                     TVVFVSGFAQLI"
     gene            1254..1838
                     /locus_tag="PLC71_09175"
     CDS             1254..1838
                     /locus_tag="PLC71_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRM02850.1"
                     /translation="MADEKMIITIGREHGTMGDKVAEKIRRQFELPLYDKAHLLRVVE
                     GTDDYDELNAFFNDRKVNSLLYAIAMSENVSSNDVPFGIIKKIIGNTGGVVMDSCGNY
                     LFRERPNTVRIFMHADKNIRIKNLMKYLGISKEQAEKEIETEDSKRYEFHKYYTNEIW
                     GMSNGYELCLDEGILGIDGCVKMILDYLKIRNLI"
     gene            1916..1987
                     /locus_tag="PLC71_09180"
     tRNA            1916..1987
                     /locus_tag="PLC71_09180"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1949..1951,aa:Glu,seq:ctc)
     gene            2383..2577
                     /gene="thiS"
                     /locus_tag="PLC71_09185"
     CDS             2383..2577
                     /gene="thiS"
                     /locus_tag="PLC71_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRM02851.1"
                     /translation="MRLNGETKTLKDPVALSDFLTQEGFSDKRIAVERNGEIVPKGEY
                     ANVILSDSDTLEIVAFVGGG"
     gene            2636..>3166
                     /locus_tag="PLC71_09190"
     CDS             2636..>3166
                     /locus_tag="PLC71_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRM02852.1"
                     /translation="MKQDKLIIGGHEFDSRFILGSGKYSLDLINAAVQNAGAQIITLA
                     LRRTNTNDTANILDYIPEGVTLLPNTSGARNAEEAVRIARLAREVGCGDFVKIEVIHD
                     SKYLLPDNYETIKATEILAKEGFVVMPYMYPDLNAARDLVNAGAAAVMPLGSPIGSNK
                     GICTKDFIQILIDEIDL"
BASE COUNT         1139 a          445 c          668 g          914 t
ORIGIN      
        1 aaatttgttg gatatatcaa tgccaaagct gtgaaagtta taaagtaatt taggtgaaaa
       61 aatagaattc tgtcgatgca ggattctatt tttcatttat gaaaataaat aaagtattga
      121 cattaaaagt tggtagtacc ataatattgt atacaagaaa aaagacttaa tatgaaaaac
      181 tggaggagtg tatgaaattt cttagacaat ttatgattat tttactactc tcttttttgg
      241 gagaagtgct taagatgttc ataccattgc caattccggc aagtgtgtat ggactggtgt
      301 taatgttggt atgtctgatg actggtattt tgaagacgag tcaagttaaa gaggcagcat
      361 tttttctgat tgagatcatg cccgttatgt ttattccagc agcagctgga ttaattgatt
      421 cgtggaaagt tttacagcca ttattactgc caatactagt cataactgta gtgatcacaa
      481 tatttgttat ggtagtcaca ggaaaaatag cacaaatgat agcacagaag agaggaatca
      541 aaaatgaatg aaatgttcag acaatcattg tttgcaggag taacgatcag tttgatcgca
      601 tatgagatcg gactattttt aaagagaaag ctgggattag caatattaaa tccattatta
      661 gtatctatag ttattgtgat cctggcattg aaagtatttg gaatcagtta tgaagtatat
      721 aacgcaagtg cgaaatacat atcttatttg ttgacgccag caacagtgtg tcttgctgta
      781 cctctatatg agcagttaga attactaaag aaaaataagg ttgcagttgt ttgtggaata
      841 ttatcaggaa cattagcaag tttaacagga atttttgttc tttgtatgtt atttggattc
      901 aaccaccaac agtatgtaac aatgcttcct aaatcgatca caacagcgat cggaattgga
      961 atatcagaag aattaggtgg aatcgtgaca attacagttg cagtgatcat tattactgga
     1021 attcttggaa atgtgatcgc agatacagta tttcatatat ttaaaataaa aaatccattg
     1081 gcaaaaggcc tggcacttgg aacagcatct catgcgatcg gaacggcaag agcaattgag
     1141 atgggaccag tcgaaggtgc aatgagcagt ctggcagtag cagtagcagg acttttgact
     1201 gttgtatttg tatcaggatt tgctcagctt atttaaaata ggaggaagaa atcatggcag
     1261 atgagaaaat gatcatcacg atcggaagag aacatggaac aatgggagat aaagtagcag
     1321 aaaaaattcg aagacaattt gaactgccac tttatgacaa ggcacatctt ctaagagtag
     1381 tagaaggaac ggatgattat gatgaattaa atgcattttt taatgacaga aaagtaaaca
     1441 gtctgttata tgcgattgca atgagtgaga atgtaagttc taatgatgtg cctttcggga
     1501 ttatcaagaa gattattggt aatacgggag gcgtcgtgat ggatagctgt ggaaattatc
     1561 ttttcaggga acgtccgaat acggtaagaa tttttatgca tgcagacaag aacattcgaa
     1621 ttaaaaatct tatgaaatat cttggaataa gcaaagaaca agctgaaaaa gaaatagaaa
     1681 cagaagattc aaaacgttat gaatttcata aatattacac aaatgaaata tggggaatgt
     1741 ccaatggata tgaattatgt ctggacgaag gaatccttgg aatcgatgga tgtgtaaaaa
     1801 tgattttgga ctatctgaaa ataagaaatt taatttaaaa aagttcttga cattacaaaa
     1861 ataatatgta taatagatag aggtggttgc aacagaccac ctcgaatata aatctggctc
     1921 catggtcaag cggttaagac gcgaccctct caaggtcgaa tcacgagttc gattctcgtt
     1981 ggagtcataa gctacaaact tcgtatttat gcggtttgta gcttttttat tttgtttata
     2041 tttgaattgg ttaaagaaaa gggttaaagt ttttgaaata ttaaatctgg aataaaaata
     2101 taacgatcaa gaaataaaaa atccccctgg catataaaat gccaaagagg attcaaacaa
     2161 atgtcaaagt gctgattaaa gcacaaagat attatatcac acaaaaaaca aaatgcaata
     2221 atcacttgac aaaaaaatat caatatgata atctacaaca agagtgaatc tgaactatcg
     2281 ggtccatgaa ggggcacacc acctcgggtg tgatgcttct ttgtggacct tttttttgtt
     2341 ccttttaaag ataaaaggaa agtcagtcaa ggaggaaagc agatgagatt aaatggagag
     2401 acaaaaacat taaaagaccc tgtcgcatta tctgatttct taacacagga aggattttca
     2461 gataagagaa tcgcagtgga aagaaatgga gaaatcgttc ctaaagggga atatgcgaat
     2521 gtgatcttaa gtgacagtga cacattagag atcgtagcct ttgtcggtgg cggatgatct
     2581 gaagataaca gatacaaaga gataagaaaa ttatgcatat ataaaggaga ataacatgaa
     2641 acaggacaag ttaatcatcg gtggacatga atttgattca agatttatct taggatcagg
     2701 aaaatattcc ttagatctga tcaatgcagc agtgcagaat gcaggagcac agatcattac
     2761 attagcactt cgaagaacga acacaaatga tacagctaat attcttgact atattccaga
     2821 aggtgtcaca cttcttccaa atacatctgg agcaagaaat gcagaagaag cagttcgtat
     2881 tgcaagactt gcaagagaag taggatgcgg agattttgtt aagattgaag ttattcatga
     2941 ttctaaatat ctgcttccag ataactatga aacgatcaag gcaacagaaa tccttgccaa
     3001 agaaggattt gttgttatgc catacatgta tccagactta aatgcagcca gagatcttgt
     3061 aaatgctggg gcagctgcag ttatgcctct tgggtctcca attggatcta acaaaggaat
     3121 ctgtacgaaa gactttatcc agattttgat cgatgagatc gatctt
//
LOCUS       DAOMUE010000237         3140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8799_length_3140_cov_2.373744, whole genome shotgun sequence.
ACCESSION   DAOMUE010000237 DAOMUE010000000
VERSION     DAOMUE010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8799_length_3140_cov_2.373744"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..899
                     /locus_tag="PLC71_09195"
     CDS             159..899
                     /locus_tag="PLC71_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM02853.1"
                     /translation="MNRSLKGICFTVAGGCCWGMSGVMGKYLFDAKDLTATWLVTVRL
                     LGAGICLLLLMYQRKGNSIFRVWKREDSAVNQLIFGMIGMAMCQMTYFLAIQESNPGT
                     ATVIQYSAPILIMVFFVLVEKRFPKKEEVLVLAAVIVGIFLLATHGSIKNLVITNAAL
                     FWGLLSAFAFAVYNVQPKKLLDEFGTLETTGWGMFVGGVVFLGTIVAFSCYLHGISML
                     GPVQGSMLGCVIRHYGFVFEGKKVLIGE"
     gene            1067..1459
                     /locus_tag="PLC71_09200"
     CDS             1067..1459
                     /locus_tag="PLC71_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRM02854.1"
                     /translation="MEELTRLEERIMQCIWDYQGEVPFLDLIETVKEKLNKDYKRTSI
                     RGYLFRLEEKGYIKVTKKGRKAYVQPLIDEETYGKYQAEKVLDRWFDGSAKRLVSALG
                     EKLTKEDGEELRGILDDFDFDDNHNKNI"
     gene            1524..2810
                     /locus_tag="PLC71_09205"
     CDS             1524..2810
                     /locus_tag="PLC71_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HRM02855.1"
                     /translation="MIEHKGYIDINYLIWKMVLLMFAIPITLIYANGFEKSTYVFEVT
                     GPIKWVIGILMIIWLVGTIIFTIRFLEKYRAIRKDILNADPCGDEIQSMVKSISKKLG
                     VHKEIQVVILEKAGGPMLYGGLKPRIILPRIYEPQQLNMIFTHELIHYKHHDLIWKHL
                     ANIICCIYWFQPALKDVFYQLDQWGETYCDRTVCEYLPDVKGYFSTIIDISMEEIRYG
                     RYTTAGLYESKEVLKLRMERMKSYRQQRRLRRGISVSVLFGMIILSATTVFASGIGFV
                     KAYDKIADETREEINVGTEGDIKDQKAKYRMYDKTRIKKCMIKNERLKENDGKPFTWK
                     IKPKERIETKEGHLKKGTCVQVAACMGFEEEHPETHIAIGIIDEDGEETYIENGVNLV
                     SHLKVKKDGRYRVFLENLGAKKAIAGGHYMVITEEE"
     gene            2823..>3140
                     /locus_tag="PLC71_09210"
     CDS             2823..>3140
                     /locus_tag="PLC71_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02856.1"
                     /translation="MFVKKVLMIVLVSVIYLINLNIPVAAEENEQDIIFSPNWMLENG
                     YKEEVEENQEILPFATRVNVNWKVGLKTEKKTKTFHKNAGSKITVEASVFPTKKIRIG
                     IVAK"
BASE COUNT         1164 a          374 c          720 g          882 t
ORIGIN      
        1 gataagattc aaaattacct agaaaatgta gataagactt ttgtatacgc caatccaaac
       61 aagaaaaata tttccattca ggaatttgaa aaatgtgaat ttgataattt actcacagaa
      121 gaatagataa aaatacagat acgaggagaa atgtatttat gaatcgaagt ttaaaaggaa
      181 tctgcttcac agttgcagga ggatgctgct ggggcatgtc tggtgtgatg gggaaatatt
      241 tatttgatgc aaaagatctg acggctacgt ggcttgtgac agtcagatta ttaggggcag
      301 gaatctgcct gctgttgctt atgtatcaga gaaaagggaa ttcaattttt cgtgtatgga
      361 aaagagaaga ttcagcggtc aatcagctga tcttcgggat gattggaatg gctatgtgtc
      421 agatgacgta cttccttgcc attcaggagt caaatccggg aactgcgaca gttattcaat
      481 attctgcacc aatcttaatt atggtgttct ttgtattagt ggaaaaaaga tttccaaaga
      541 aagaagaagt gcttgttctg gcagctgtga tcgtaggtat ctttttgctt gcaacacatg
      601 gaagtataaa aaacttagtg atcaccaatg cagcattatt ctggggattg ttatccgctt
      661 ttgcttttgc agtttataac gtgcagccaa agaaattatt agacgaattt ggtactttag
      721 agacaacagg atggggaatg tttgtcggtg gagttgtatt cttaggaacg atcgtggcgt
      781 tttcctgtta tctccatggg atttccatgt taggaccagt tcagggaagt atgcttggat
      841 gcgtaatcag acattatgga tttgtctttg aaggtaagaa ggttttgatc ggagaatagt
      901 agataaatcg ctgagttcat atgaattata tggattcagt gattttttca tagaatgatt
      961 attttattga aaaaaataac ctgtaaatat attgtgttaa atacaaaaca atgttaaaat
     1021 agaagtaacc taaaaataga acataaaaga aaggaggaat acgaaaatgg aagaattaac
     1081 cagactagaa gaacgaatca tgcaatgcat ttgggattat caaggagaag ttccttttct
     1141 tgatctgata gagacagtca aagaaaagct caataaagat tataaaagaa caagtatccg
     1201 cggatatctg ttccggctag aagagaaggg atatatcaaa gttaccaaga aaggaagaaa
     1261 agcatatgtg cagccattga tcgatgaaga aacatatggc aaatatcaag ctgagaaagt
     1321 tttagatcgt tggtttgacg gatctgcaaa acgattagta agtgcattag gagagaaact
     1381 gacaaaagaa gacggggaag agttaagagg gatcttagat gactttgatt ttgatgataa
     1441 tcataataaa aatatttagc ggaatattct tatgtacact gcttggaacg atcttatatc
     1501 ttttttggaa ggctgtagga cgtttgatag aacacaaggg atatattgat atcaattatc
     1561 tgatatggaa gatggtatta ttgatgtttg ccataccaat cacgttgatc tatgcaaatg
     1621 gatttgagaa gagtacttat gtatttgaag ttacaggtcc gatcaagtgg gtcattggaa
     1681 ttttgatgat catctggctg gttggaacga tcatttttac gatcagattt ctagagaaat
     1741 acagagcaat aaggaaggat attttaaatg cagatccatg tggagatgaa attcaaagta
     1801 tggtaaaaag catatcaaaa aagttagggg ttcataaaga aattcaagta gtgatcttag
     1861 aaaaagcagg cggaccaatg ttatatgggg gtttgaaacc aagaatcatt ctgccaagaa
     1921 tttatgaacc acaacagtta aatatgatat ttacacatga attgattcat tataaacatc
     1981 atgatctcat ttggaaacac ttggcgaaca ttatctgttg tatatactgg tttcaacctg
     2041 cgttaaagga tgttttttat caattagatc aatggggaga aacatactgt gacaggacag
     2101 tatgtgaata tctgccagat gtaaaaggat atttttctac gatcatagat atttctatgg
     2161 aagagatcag atatggaaga tatacaacag cggggttata tgagagtaag gaagtattaa
     2221 agttacgaat ggaacgaatg aaatcttatc gacaacaaag aaggttgcga agaggaattt
     2281 ctgtaagtgt cttatttgga atgatcatac tcagtgcaac tacggtattt gcttcaggga
     2341 taggatttgt aaaggcatac gataagattg cagatgaaac aagagaggaa atcaatgttg
     2401 gaacggaagg tgatataaaa gatcagaaag caaagtatag aatgtatgat aaaaccagaa
     2461 tcaaaaagtg catgataaaa aatgaaagat taaaagagaa tgatggaaaa ccatttacat
     2521 ggaagatcaa gccgaaagag agaatagaga caaaagaagg acacttaaaa aagggaactt
     2581 gtgttcaagt ggctgcctgt atggggtttg aagaagaaca ccctgaaaca catatagcaa
     2641 ttggaattat tgatgaggat ggagaggaaa cttatataga gaatggggta aatctggtaa
     2701 gccacttaaa ggtaaaaaaa gatggacggt atcgggtatt tttagaaaat ctaggggcga
     2761 aaaaagcgat agcgggtgga cattatatgg tgataacgga agaagagtag aggagagaaa
     2821 agatgtttgt aaaaaaggta ttaatgatag tgcttgttag tgtaatatat ttaattaatt
     2881 taaatattcc agtagctgca gaagaaaatg aacaagatat tattttttca cctaattgga
     2941 tgttagaaaa tggatataag gaagaagtag aggaaaatca agaaatatta ccatttgcta
     3001 caagagttaa tgtaaactgg aaagttggac taaaaacaga aaagaaaacg aaaacatttc
     3061 ataaaaatgc aggaagtaaa attactgtag aggcaagtgt gtttccaaca aagaaaatac
     3121 gtataggaat tgtagcaaaa
//
LOCUS       DAOMUE010000238         3135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8822_length_3135_cov_1.781169, whole genome shotgun sequence.
ACCESSION   DAOMUE010000238 DAOMUE010000000
VERSION     DAOMUE010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8822_length_3135_cov_1.781169"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..98
                     /locus_tag="PLC71_09215"
     CDS             <1..98
                     /locus_tag="PLC71_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003459936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM02857.1"
                     /translation="VERMMRDAKITQIYEGTNEIQKLIVSGAIFR"
     gene            310..732
                     /locus_tag="PLC71_09220"
     CDS             310..732
                     /locus_tag="PLC71_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRM02858.1"
                     /translation="MELTQCINYLLTTAQHKVFQEMSSRLEKFDVTPVQYGVMYCLWI
                     EGKTKPKEIAAELKLENSTISGVLERMEKKELIERQVSKEDRRFIEVVLTEKGKALQN
                     PILATVEEVNKDVLSEMSQEEQSELKKALRILGGLEDE"
     gene            766..1794
                     /locus_tag="PLC71_09225"
     CDS             766..1794
                     /locus_tag="PLC71_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM02859.1"
                     /translation="MRVTIRQIAEESGVSRGTVDRVLNNRGKVRPEVEERVRKVAEEL
                     GYKPNLLGRALIKMKENIKIGVILQDTGTPYLKTLSDGIKKAKGEVEDFGGTVIVKAV
                     DYGNVEQTLAAMNKLKEAGIKGLVLMPINDKRVLQKIDQFTEEYGISVVTVNADVEDT
                     KRICFVGQNSVQSGRAAAGLMHDILREEGTIAVISGIETNTSLSDRIYGFCDEMKKIS
                     PKTEILDTKYCFEDDLIAAHLTESILNRYEDLSAIYITCHGEKGVCDTLRKHKKMKKV
                     TIIACDLSRQNYRYLKNGDIKYLIGQDGRYQGYEAVMTLYRLLFNDEPPKKEHKYTDI
                     IVKTKYNI"
     gene            2006..3019
                     /gene="iolG"
                     /locus_tag="PLC71_09230"
     CDS             2006..3019
                     /gene="iolG"
                     /locus_tag="PLC71_09230"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HRM02860.1"
                     /translation="MVNVGIIGAGRIGQVHMNSILTRVPNAKIKAVADPFLSEEADKK
                     AKAAGAEYTTKDYKDILADKDIDAVIICSSTDTHSPISIEAIKAGKHVFCEKPIDHDV
                     DKIKEVIKTLEGTGLKYQVGFNRRFDHNFEAAQRAVVNGKIGAPHIIKITSRDPEPPS
                     AEYVKVSGGMFLDMTIHDFDMVRFLAGCDAEEVYVQAANLVDPEIGKAGDVDTAIITL
                     KMENGALAVIDNSRQAVYGYDQRAEVFGSKGMVAIKNDSDSTAVISNEDGVTGEKPQF
                     FFLERYMDAYGKEMVQFIDAIENDTETPLGVEDGLKPVLMGLAAKKSVEEGRPVKISE
                     IEF"
BASE COUNT         1194 a          455 c          717 g          769 t
ORIGIN      
        1 atgtagaacg tatgatgaga gatgctaaga tcacacagat ttacgaagga acaaatgaaa
       61 tccagaaact gatcgtaagc ggagcaatct tccgatagaa aagaatataa aattaaaaat
      121 ttcttctcct taagtaaaaa gttatcggat aggtttgaac tatctgataa cttttttcta
      181 tttcttcata gaaaatttta atgaaatcta ctatgaaaca aaaaaggaga gaaaactaaa
      241 ttacttgaca cataaaaaaa agcaagctac actaatatta gtgtgcgaaa tatttaaagg
      301 agaaattata tggaactaac acaatgtatt aattatttat taacaacagc acagcataaa
      361 gtttttcagg agatgagttc cagacttgaa aagtttgatg taacaccagt tcagtatggt
      421 gtcatgtatt gtttatggat cgaaggaaaa acaaaaccaa aagagatcgc agcagaatta
      481 aagctagaga actctacgat ctccggagtt ttagaacgta tggagaagaa ggaactgatc
      541 gaacgtcagg taagcaaaga agaccgtaga tttatagaag ttgtattaac agagaaagga
      601 aaagcattac agaatccgat ccttgcgacg gtagaagaag tgaataaaga tgtattgtct
      661 gagatgtcgc aggaagaaca aagtgaactg aagaaagcct tgaggattct gggtggactg
      721 gaggacgaat aaagagaact ctggtcatga cagacaggga taagtatgag agtaacaatt
      781 cgacaaattg cagaagaatc aggagtttcc agaggaacgg tagaccgtgt attgaataac
      841 aggggaaaag ttcgtccaga agtagaagaa cgagtccgta aagtagcaga ggagcttgga
      901 tataagccaa atcttttagg tcgtgcattg atcaagatga aagagaatat caagatcggt
      961 gtgatccttc aggatacagg aacgccttat ttaaaaacat tatcggatgg gatcaagaag
     1021 gcaaaaggcg aagtagaaga ttttggtgga actgtcattg tgaaagcggt agattatggt
     1081 aatgtagaac agaccttagc ggcaatgaat aagctgaaag aagcagggat caaagggctt
     1141 gtgctgatgc cgatcaatga taagagagtt cttcagaaga tcgatcagtt tacagaagaa
     1201 tatgggatat ccgttgtcac agtgaatgca gatgtggaag atacaaagag aatttgtttt
     1261 gttggacaga actctgtaca aagcggcagg gcagcagcag gattaatgca tgatattcta
     1321 agagaagaag gaacgatcgc agtgatctct ggaattgaga cgaatacttc attaagtgac
     1381 agaatctatg gattttgtga cgagatgaag aaaatttcac caaagactga gatattagat
     1441 acaaaatact gctttgagga tgatctgatc gcagcacatc tgacagagag tattttgaac
     1501 cgttacgaag atttatctgc gatttatatt acctgtcatg gagaaaaagg ggtatgtgat
     1561 acccttagga aacataagaa gatgaagaaa gtgaccatca ttgcatgtga cctgtcacgg
     1621 cagaattaca gatatctaaa gaatggagac atcaagtatc tgatcgggca ggatggacgt
     1681 tatcaaggat atgaagctgt catgacctta tatcggttat tatttaatga tgagccgccc
     1741 aaaaaagaac ataagtatac agatattatt gtaaaaacaa agtacaatat ttaatgatgt
     1801 aagtgagaat aagaacaaga tcagggaaat atccttggtc ttgttcttat tttcgtacat
     1861 ttgcacaaaa tttaggaaga atttttgtgc agtttaccaa ttgtgtgctc gaacacaaag
     1921 tgctataatt caaaacataa aagatgtgct cgaacacaaa acacaaaaaa caaatcaaaa
     1981 gcgtattaga aaaggagaaa caattatggt aaacgtagga attattggag caggaagaat
     2041 cggacaggta cacatgaaca gcattttaac aagagtacca aatgcaaaga tcaaagcagt
     2101 tgcagatcca ttcttaagtg aagaagcaga caagaaagca aaagcagctg gagcagagta
     2161 tacaacaaaa gattacaaag atatcttagc agacaaagat attgatgcag tgatcatttg
     2221 ttcatcaaca gatactcatt ccccaatctc aattgaagcg atcaaagcag gaaaacatgt
     2281 attttgtgaa aaaccgatcg atcatgatgt agacaagatc aaagaagtta tcaaaacatt
     2341 agaaggaaca ggacttaaat atcaggtagg atttaacaga agatttgacc acaactttga
     2401 agcagcgcag agagctgttg tcaatggaaa gatcggagca cctcatatca tcaagatcac
     2461 atcaagagat cctgagccac caagtgctga atatgtaaaa gtatctggag gaatgttcct
     2521 tgatatgaca attcatgatt ttgatatggt acgattcctt gcaggatgtg atgcagaaga
     2581 agtttatgta caggcagcaa accttgtaga tcctgagatt ggaaaagcag gtgatgttga
     2641 cacagcgatc atcacattga agatggaaaa tggagcactt gcagtaatcg ataactctcg
     2701 tcaggcagtt tatggatatg atcagagagc agaagtattc ggatctaaag gaatggtagc
     2761 gatcaagaat gattctgatt ctacagcagt gatcagcaac gaagatggtg taacaggaga
     2821 aaaaccacag ttcttcttcc ttgaaagata catggatgca tatggaaaag aaatggttca
     2881 gttcattgat gcaattgaaa atgacacaga aacaccatta ggagtagaag atggattaaa
     2941 accagtactt atgggacttg cagcaaagaa atcagtagaa gaaggccgtc cagtaaaaat
     3001 ttcagaaatt gaattctaat ttgaaggaga gtgtatatgg aaaaacatac aaatgaggtt
     3061 gaagacaatc agatgagagc acttagctgg aagaccagag tgtcttacgg cttaggtgat
     3121 acagcgtgta acgtt
//
LOCUS       DAOMUE010000239         3124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8872_length_3124_cov_2.118931, whole genome shotgun sequence.
ACCESSION   DAOMUE010000239 DAOMUE010000000
VERSION     DAOMUE010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8872_length_3124_cov_2.118931"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="PLC71_09235"
     CDS             complement(<1..527)
                     /locus_tag="PLC71_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM02861.1"
                     /translation="MNNMKLPVGISDFHKLRQNEYYYVDKTNLIKELLESRIAEVTLI
                     TRPRRFGKTIGMSMLAYFFDIRKDSRKLFEDLKISRDIELCQKWMNQYPTLFISFKDV
                     DGLNFQSAMKMLRNQISWICNEHDYLSDSDKVNENDKKIFLKLQDISEENLSEDLIKI
                     SVATIIRMMNVHYGKP"
     gene            complement(551..>676)
                     /locus_tag="PLC71_09240"
     CDS             complement(551..>676)
                     /locus_tag="PLC71_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02862.1"
                     /translation="VSCSIGVYRFTYPKTIKELLTKTDEILYEAKANGRGCFVIR"
     assembly_gap    677..686
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<687..1888)
                     /locus_tag="PLC71_09245"
     CDS             complement(<687..1888)
                     /locus_tag="PLC71_09245"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM02863.1"
                     /translation="MKQNKWKHLKFEKYVFVLLLAIELIMSFTFLGFIHIDPISLTTA
                     YIPIVVAGCLLGPLESTLIGLVFGLASMYKASALYVVSDDKIFSPLYSGNPIGSILLS
                     VGSRVLFGFVIGYLFSIIKGRKYEKIGIWILSLISQWIHAFLVFTVMGACFPQLGYTG
                     MSTFHMGINDALIALCCLFWVEVAYKIYHKERIQKYKDAVNQSLDDPYLSSKIVAISS
                     GISFLIICIAIFSTGYFANRSTYMLKQHRVHVTRTIEIDLLHLQVQFLIAMLALDFIL
                     ILTMLMIYQYMKYREYQGEIDFLTGVMGRRLFLQHCQNIQSDQRIHGHQGWFLFLDVD
                     WFKQINDKLGHIAGDETLKKFAMFLKEQFEPYGAVGRVGGDEFAVMIEEEMSQEALEE
                     EMKQFFQN"
     gene            complement(1885..2166)
                     /locus_tag="PLC71_09250"
     CDS             complement(1885..2166)
                     /locus_tag="PLC71_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HRM02864.1"
                     /translation="MQSAYIHLYLIMINVITFALYGLDKWRAMTRQWRVREKKLIILA
                     LIGGSIGAMIGMTVFHHKTRKWYFRLDVSKQRRIGKKFKRPKSKGRLEI"
     gene            complement(2193..2756)
                     /locus_tag="PLC71_09255"
     CDS             complement(2193..2756)
                     /locus_tag="PLC71_09255"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM02865.1"
                     /translation="MIANTLIHREFTSSYTAKFVIEKDRMYTENANRSSGDGIITPDN
                     MEPNPKNPIIASFFRNIGWSDRLGSGVRNIFKYSKYYSGEEPEFVEGDVFRLIVPLNE
                     DYSYDNVKNGDKKTAIKNGDKKISKKTIQNYKKILEFMEEGKEYTIQDFCNLLNLKPS
                     RTKELLKALTQDIEQIGNNKNRKYRLK"
     assembly_gap    2870..2879
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2942..>3124)
                     /locus_tag="PLC71_09260"
     CDS             complement(2942..>3124)
                     /locus_tag="PLC71_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRM02866.1"
                     /translation="DDLIMDICPAYETDALVRRVNVDRYDDREIIRTNLIESYDQLME
                     FGRKHLSDKFFIEGVE"
BASE COUNT          920 a          672 c          382 g         1130 t
ORIGIN      
        1 ggctttccgt aatgcacatt catcattctt atgatggtag ccacactaat ttttatcaaa
       61 tcttctgaca aattttcttc acttatatct tgtaacttca aaaagatctt tttatcattc
      121 tcatttacct tatcgctatc tgataaataa tcatgctcat tgcagatcca tgaaatctga
      181 tttcttaaca tcttcatagc tgattgaaaa ttcaatccat ctacatcttt gaaggaaata
      241 aataatgttg gatactgatt catccatttt tgacataatt ctatatctct tgagatcttc
      301 aaatcttcaa atagtttcct gctgtctttt cgtatatcaa agaaatacgc caacatactc
      361 atgccgatcg tctttccaaa gcgtcttggt ctcgtgatca atgtaacttc tgcgattctt
      421 gattccagta attctttgat caaattcgtt ttatctacat aataatattc attttgtctt
      481 aatttatgaa aatcagaaat tcctactggt aatttcatat tgttcattgc caacaccacc
      541 ttcttagact ttatctaata acaaaacatc ctcttccgtt tgcttttgct tcatataaga
      601 tctcatcggt ctttgtcaga agttccttga tcgtttttgg atatgtaaaa cgatatactc
      661 caatactgca gcttacnnnn nnnnnnatgt tttgaaagaa ttgtttcatt tcttcttcta
      721 acgcttcctg gctcatctct tcttcgatca tcacagcaaa ctcatctcca ccaactcttc
      781 cgactgctcc atatggctca aattgttctt ttaaaaacat cgcaaatttc tttaaggttt
      841 catctcctgc tatatgtccc agtttatcat tgatctgctt aaaccaatcc acatccaaga
      901 ataaaaacca tccttgatgg ccatgaatcc tctgatcact ttgaatattt tggcaatgct
      961 gcaaaaacag tcttcttccc atcaccccag taaggaaatc aatctcccct tgatattccc
     1021 gatacttcat atattggtag atcataagca tcgtcaatat taagataaaa tccaatgcaa
     1081 gcattgcgat caaaaactgt acctgtaaat gtagcagatc aatctctatt gttcttgtta
     1141 catgaactct gtgctgtttc aacatatatg tagaccgatt tgcaaaatat ccagtagaaa
     1201 atatcgctat acaaatgatc aaaaaactga tccctgatga tatagcgact atcttagaag
     1261 acagatatgg atcatccaaa gattgattta ccgcatcctt gtatttttga atgcgttcct
     1321 tgtgataaat cttatatgcc acctctaccc agaaaagaca acatagtgca ataagtgcat
     1381 cattgatccc catatgaaat gttgacattc cagtatagcc aagttgtgga aaacaggctc
     1441 ccataacagt gaacactaag aaagcatgta tccactgtga gatcaaagaa agaatccaga
     1501 ttcctatttt ctcatacttt cgtcctttta tgatggaaaa cagatatccg atcacaaagc
     1561 caaacagaac tcttgaccca acacttagta aaatgcttcc tattggatta ccactgtata
     1621 gcggcgaaaa tatcttatca tctgatacaa cataaagtgc tgatgcctta tacatacttg
     1681 ctagtccaaa taccaatccg atcagtgtcg attccaaagg tccaagcaaa catcccgcaa
     1741 ctacgatcgg gatatatgct gttgtaagtg aaattggatc aatatggatg aatcctaaaa
     1801 aagtaaatga cataataagc tcgatcgcca ataataacac aaaaacatat ttttcaaatt
     1861 ttaaatgctt ccatttgttt tgcttcatat ctctaatctt cctttgcttt tgggtctctt
     1921 aaacttcttc ccaattcttc tttgcttact tacatctaat ctaaaatacc attttcttgt
     1981 cttatgatga aatactgtca taccgatcat tgctccaatg gagcctccga tcagtgcaag
     2041 tatgatcaat tttttctctc tgactctcca ctgtcttgtc atcgctctcc atttgtctag
     2101 tccatacaat gcaaatgtta taacgttgat catgatcaga tataagtgaa tatatgctga
     2161 ttgcatttgt aggtttcctc cccatagcct ttttatttta atctgtattt tcgattttta
     2221 ttattgccta tttgttctat atcttgtgtc aaagctttta ataactcttt cgttctggag
     2281 ggttttaaat ttaacaaatt acaaaaatcc tgaatcgtat attctttacc ctcttccata
     2341 aattcaagta tttttttata attttgtatt gtcttttttg aaatcttttt atcgccgttt
     2401 tttatcgccg tttttttatc gccgtttttt acattatcat atgaataatc ttcatttaat
     2461 ggcacaataa gcctaaatac atccccttct acaaattctg gttcttctcc tgaataatat
     2521 ttcgaatatt taaatatatt tctcactcct gaacctaatc gatctgacca tcctatattt
     2581 ctaaaaaatg aagcaataat cggattcttt ggatttggtt ccatattatc aggtgtaata
     2641 attccatctc ctgatgaccg attcgcattt tctgtataca tcctatcttt ttctattaca
     2701 aatttagctg tatatgaact cgtaaattct ctatgaatca atgtatttgc aatcatttcc
     2761 caataacata actgctgcca gattatatcc actttctcct gtcgctctgt cctttccata
     2821 taatcccgca cttctcaata attcctcatc actcatactt tcccaactan nnnnnnnnna
     2881 attccctatg aatcaatgtg tttgcaatca tttccttggt tataatattc ctcagatttt
     2941 ttcattccac cccttctata aaaaatttat cagacaaatg ttttcttcca aattccatta
     3001 attgatcgta actttctatt aagtttgttc taatgatttc tcgatcatcg tagcgatcca
     3061 cattaactct tcttaccaac gcatccgttt cataagctgg gcaaatatcc atgatcagat
     3121 catc
//
LOCUS       DAOMUE010000240         3121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8887_length_3121_cov_2.302674, whole genome shotgun sequence.
ACCESSION   DAOMUE010000240 DAOMUE010000000
VERSION     DAOMUE010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8887_length_3121_cov_2.302674"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLC71_09265"
     CDS             complement(<1..405)
                     /locus_tag="PLC71_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HRM02867.1"
                     /translation="MAEVKKIATRESYGNALVELGQENPNVVVLDADLAAATKTGMFK
                     KAFPERHIDCGIAECNMIGIAAGLAAAGMTPFASSFAMFAAGRAFEQVRNSIGYPHLN
                     VKIGATHAGITVGEDGATHQCNEDIALMRTIPG"
     gene            complement(407..1228)
                     /locus_tag="PLC71_09270"
     CDS             complement(407..1228)
                     /locus_tag="PLC71_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRM02868.1"
                     /translation="MTTAELKKTANEVRKGIIKSTHAAKSGHPGGSLSAADVFTYLYF
                     VELNVDPKNPKDENRDRFVLSKGHVAPGYYSTLAERGFFPKEDLLTLRHVGSYLQGHP
                     DMKKIPGVDMSSGSLGQGISAAVGMALAAKLQNKDYRTYTLLGDGEIQEGQVWEAAMF
                     AGSRKLDNLVVIVDNNGLQIDGPIDEVNSPYPIGAKFEAFNFNVVEIDGHDFDQIADA
                     FKQAKECKGKPTAIIMKTIKGKGVSFMENQVSWHGSAPNDEQCKQALEELEKVGE"
     gene            complement(1247..1903)
                     /gene="fsa"
                     /locus_tag="PLC71_09275"
     CDS             complement(1247..1903)
                     /gene="fsa"
                     /locus_tag="PLC71_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HRM02869.1"
                     /translation="MRFFVDTADVDQIRKANDMGVICGVTTNPSLIAKSGRQFEDVIK
                     EITSIVDGAISGEVKATTTDAEGMIKEGREIAKIHPNMVVKIPTTIEGLKATKVLSSE
                     GIKTNLTLIFNPNQALLAARAGATYVSPFLGRLDDISQNGLDLIADIREIFDAAGDID
                     TQIICASVRNPIHVTECAKMGADIATVPYNVFEQMMKHPLTDQGIEKFQADYKAVFGE
                     "
     gene            2260..>3121
                     /locus_tag="PLC71_09280"
     CDS             2260..>3121
                     /locus_tag="PLC71_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRM02870.1"
                     /translation="MGLKKKVTTKDIADHVGLSQSAVSMILNNKTNVSFSDSTRQKVL
                     DAAKELGYQKKKKSIEQTSKPLSKIILIMCPFLSNHYYTTLVHSITERAHDYGYLTFV
                     APTLRNPSTEQYYLDLMKNDLDTAGIVYLYPTSFLPEVNDMSKQIPIVNIGDKNDEIT
                     FDSIHVSSSKPARLVAEHLISLGHRKITYISTPISDIERSRSKRYDALVATFKEHGFG
                     DDCVSIRSLSPGEYNLLSPNMLEYSSGYNLTKQILEEGTDSTAFVGYNDMVAFGILDA
                     LYELKYKVPND"
BASE COUNT          866 a          672 c          520 g         1063 t
ORIGIN      
        1 gccggggatg gtgcgcatca gggcgatatc ctcgttacac tgatgggtag cgccatcctc
       61 acctactgtg atacctgcat gggttgcacc gatcttcaca ttcagatgcg ggtaaccgat
      121 agagttacgt acctgctcga atgcacgtcc tgctgcaaac atcgcaaaac tacttgcaaa
      181 tggagtcatt cctgctgctg caagtccagc agcaataccg atcatattac attctgcgat
      241 accacagtca atatgtcttt ctggaaaagc tttcttaaac attcctgttt ttgttgcagc
      301 ggctaagtca gcatctaaaa ctacaacatt agggttttcc tgtcctaatt caactaaagc
      361 attaccatag ctttctcttg tcgcaatctt ctttacttct gccatcttac tcgcccacct
      421 tttctaattc ttccagtgcc tgtttacatt gttcatcatt tggtgcagaa ccatgccatg
      481 aaacctgatt ctccataaag gatacacctt tacctttgat cgtcttcata atgatcgctg
      541 ttggttttcc tttgcattct tttgcctgtt tgaacgcatc agcgatctga tcaaagtcgt
      601 gaccatcaat ttcaaccaca ttaaagttaa atgcttcgaa tttagctccg atcggatatg
      661 gagagtttac ttcatcaatt ggtccatcaa tctgtaatcc gttgttatca acgataacta
      721 ctaaattatc aagttttctg gatcctgcaa acattgcagc ttcccatacc tgtccttcct
      781 ggatctcacc atcaccaaga agtgtatatg tacgatagtc tttattctga agttttgcag
      841 caagtgccat accaactgcg gcagagattc cctgtcctaa tgatccacta gacatatcta
      901 cgcctggaat cttcttcata tcaggatgtc cctgtaagta agatcctaca tgtcttaatg
      961 ttaacagatc ttcttttggg aaaaatcctc tttctgctaa tgttgagtaa tatccaggag
     1021 ctacatgtcc tttagataat acgaaacgat ctctgttttc atcttttgga ttcttaggat
     1081 caacgtttaa ctcaacgaaa tataaatatg taaatacatc agctgctgat aaagatccac
     1141 ccggatgtcc ggatttggca gcatgtgttg acttaatgat acctttgcga acctcatttg
     1201 ctgttttctt taattctgcg gttgtcattg tatctaccac cctttcttat tctccgaata
     1261 ctgctttgta atctgcctgg aatttttcga ttccctgatc tgttaatggg tgtttcatca
     1321 tctgttcaaa tacgttgtaa ggtactgtgg caatgtctgc tcccatctta gcacattctg
     1381 ttacgtggat tgggttacgt acacttgcac aaatgatctg tgtatcgata tctcctgctg
     1441 catcaaagat ctctctgatg tctgcaatta aatctaatcc gttctgagaa atgtcatcca
     1501 gtcttcctaa gaatggagat acatatgttg ctccagctct tgctgctaat aaagcctggt
     1561 ttggattgaa gatcaatgtc agatttgttt tgatcccttc agaagataat acctttgtag
     1621 cttttaatcc ttcgatcgtt gtaggaatct taactaccat gtttggatga atcttagcga
     1681 tttctcttcc ttctttgatc attccttctg catcagttgt tgtagctttt acttcaccac
     1741 tgatagctcc atctacaata gaagtgatct ctttgatcac atcttcaaac tgtcttcctg
     1801 atttagcgat cagtgaaggg tttgttgtaa ctccacagat aactcccata tcatttgctt
     1861 tacgaatctg atctacatct gctgtatcca cgaaaaatct cataacttac ttcctcctaa
     1921 aacattgttt gttgttttgt tcgttgtttt gtttatggct ttatttaaac atggataaaa
     1981 tgtcttgtca aactcgaaaa actgtcagat ttcagcaata ttcattgaat ttattaatat
     2041 ttttcaactt tattaatagt acatggtttt tcgttttttt tcaagatgct ttcttcaatt
     2101 tttttcaatt aaaaaaacct cgtttttcct tgtttttccg taaaaaaata tgctatgata
     2161 aatgtagttt taattaataa ttattatttt attactaata tttctgtttt tattaattgt
     2221 tattaattaa ttaaaataat tttgattaag gaggatttta tgggtttaaa gaagaaagta
     2281 acgaccaaag acattgcaga tcacgtcggt ctttcccagt ctgcagtctc catgatctta
     2341 aataataaga ctaatgtctc tttttcagac agcaccagac agaaagtttt ggacgctgca
     2401 aaagaacttg gctatcaaaa aaagaaaaaa tccattgaac agacatcgaa gcctttatcg
     2461 aaaatcattc tgatcatgtg tccgttctta tctaaccatt attatacaac tctggttcat
     2521 tcgatcacag aacgagcaca tgattatgga tatcttacct ttgttgcacc aacactgcga
     2581 aacccgagca ccgaacaata ttatctggat cttatgaaaa atgatctgga tactgctgga
     2641 atcgtttatc tgtatccaac ttcttttctt ccagaagtta atgatatgtc aaaacagatt
     2701 ccaatcgtca atatcgggga taaaaatgac gagatcactt ttgactcgat ccatgtcagc
     2761 agctccaaac ctgccagact cgtcgcagaa catttgatct cccttggaca ccgcaagatc
     2821 acttacatat caacaccgat cagtgatatt gaacgttcca gatcaaaacg ttacgatgca
     2881 cttgttgcaa ccttcaaaga acacggcttt ggagatgact gcgtcagtat tcgttccctc
     2941 tcacccggag agtataatct gctgtctcca aatatgctgg aatattcttc cggatacaat
     3001 ctgacaaaac agattctcga agagggaacg gattctaccg ccttcgttgg atacaatgat
     3061 atggttgcat ttggaatcct agatgcatta tatgaattaa aatacaaagt accaaatgac
     3121 t
//
LOCUS       DAOMUE010000241         3117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8906_length_3117_cov_2.404637, whole genome shotgun sequence.
ACCESSION   DAOMUE010000241 DAOMUE010000000
VERSION     DAOMUE010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8906_length_3117_cov_2.404637"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..163
                     /locus_tag="PLC71_09285"
     CDS             11..163
                     /locus_tag="PLC71_09285"
                     /inference="COORDINATES: protein motif:HMM:NF018645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRM02871.1"
                     /translation="MYSGGDSGFEERDGVLEYLSQLDDRKYFIARQDFPNKKNNPPMP
                     VFIIKL"
     gene            237..1388
                     /locus_tag="PLC71_09290"
     CDS             237..1388
                     /locus_tag="PLC71_09290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRM02872.1"
                     /translation="MKTIVSVFLYVIICSIQIYAVSAEEINLNAKAAVIMDAESGKIL
                     YEKSKDQKMPNASTTKILTCLYILKHCDLDQMAEVSKNAAMQPKVRLGVREGEKYKVK
                     DLLYGLMLESYNDCAVVLAEHAEGSVENFEKRMNQMAENLGTLNTHFVTPNGLDGIDK
                     KGRHETTAQDLAKIMARCIKNQQFLEITQTKQYTFTDGSRKRRFICNNHNALLSSMQG
                     VISGKTGYTSKAGYCYVGAVKQKNMTMTFSVLASGWPPHKTYKWKDVRKLVQYATDHY
                     ERREIIADTSKIKEISIKNGLKEKVLLKTGNLKAAFLVKKTDKIKIESILPSFIDAPV
                     KEGQKVGEIKYLINGKSQKSVPIYAKQSVKQKDYWYYFEKIIQRFTLTT"
     gene            1432..1782
                     /locus_tag="PLC71_09295"
     CDS             1432..1782
                     /locus_tag="PLC71_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HRM02873.1"
                     /translation="MLFLEYPKCSTCKKAKKWLDEQGISYEDRHIIEDNPTVEELKDW
                     HERSGLPLKRFFNTSGMKYRELGLKDKLPEMSEEEQYELLATDGMLVKRPLVVGDDFV
                     LIGFKEALWKETLA"
     gene            1779..>3117
                     /locus_tag="PLC71_09300"
     CDS             1779..>3117
                     /locus_tag="PLC71_09300"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRM02874.1"
                     /translation="MNRENAWTTYEEADIKALEGLCKEYKDFLSNGKTERECVAQSIE
                     LAKKAGYQDLEELIKNQTPLKAGNKVYRTWMEKTIALFQIGEDAMEEGLNILGAHIDS
                     PRLDLKQNPLYEDTEMALLDTHYYGGVKKYQWVTLPMALHGVVVKKDGTKVNICIGEK
                     DEDPVVGITDLLIHLSGKQMQKKGNVIVEGEDLNVLIGSKPLKGEEKEAVKKQLLQLF
                     DEEYGIEEEDFLSAEIEVVPAGKARDFGLDRSMIMSYGQDDRVCAYTSLAAMLNVEAP
                     KRTSCCILVDKEEIGSVGATGMHSRFFENTIAEILALTGDYNDLRLRRTLQNSYMLSS
                     DVSAAFDPNYPSVMEKKNCAYFGKGLVLNKYTGARGKSGSNDANPEYIAQLRKIFDEA
                     KVAFQTAELGKVDEGGGGTIAYIPAAYSMNVIDCGVAVLSMHAPWEITSKSDIY"
BASE COUNT         1181 a          497 c          715 g          724 t
ORIGIN      
        1 gagcatctgt atttacagtg gcggtgacag tggttttgaa gaacgtgatg gggtgctaga
       61 atacctaagc cagctagatg accgtaagta ttttattgca agacaggact tccccaataa
      121 gaaaaacaat ccgccaatgc cggtatttat cataaaatta taataaagaa acaatggatt
      181 ccatatactt gtattaaatt gaaccttaaa gtaagcaaaa aagaagggta caggatatga
      241 aaaccattgt ttctgttttt ttatatgtga taatatgcag tatacagata tatgcagtgt
      301 cagcagaaga aataaactta aatgcaaagg cagctgtgat catggatgct gaaagtggga
      361 aaatcctcta tgagaaatcc aaagatcaaa aaatgccgaa tgccagtaca acaaagattt
      421 taacatgttt atatatttta aagcactgtg atcttgatca gatggcagaa gtttcaaaga
      481 atgcagcgat gcagccgaaa gtaagattag gggtgagaga aggggaaaag tataaagtca
      541 aagatctgct ctacggatta atgttagagt cctataatga ttgtgcagtt gtattagcag
      601 aacatgcaga aggaagtgtg gaaaactttg aaaaaagaat gaatcagatg gcagaaaatc
      661 taggtacttt gaatacccat tttgtaacac caaatggtct ggatggtata gataagaaag
      721 gacggcatga aacgacagca caggatctgg caaagatcat ggcgcgatgt attaagaatc
      781 agcaatttct tgaaatcacg cagacaaaac aatatacatt tacagatggc agcagaaaga
      841 gaagatttat ctgcaataat cacaatgcct tgttatccag catgcaagga gtgatttctg
      901 ggaagacagg atacacttca aaagcaggat attgctatgt gggggctgta aagcagaaga
      961 acatgacgat gacattttct gttcttgcca gtgggtggcc gccgcacaag acctataaat
     1021 ggaaggatgt aaggaaactg gttcaatacg ccacagatca ttacgagaga cgagagatca
     1081 ttgcagatac aagcaagatc aaagaaataa gtataaagaa tggtcttaaa gaaaaagtct
     1141 tgctgaaaac aggaaatctc aaggcagcat ttctggtaaa gaaaacagat aagataaaga
     1201 ttgagagtat cttaccctct ttcattgatg cacccgtgaa agaaggacag aaagtcgggg
     1261 agatcaaata tcttatcaat ggaaaaagtc aaaagagcgt tccaatctac gcaaagcaaa
     1321 gcgtaaaaca aaaagactac tggtattatt ttgagaaaat aattcaaaga tttacgctga
     1381 caacctaatg tgctatgata aaacagataa gaaaacaagg gaggcgaaac aatgctgttt
     1441 ttagaatatc caaaatgcag tacctgcaag aaggcaaaga aatggttgga tgaacaaggt
     1501 atatcttacg aagaccgtca tatcattgaa gacaacccaa cggtcgaaga gttaaaagac
     1561 tggcacgaaa gaagcggatt gccactgaag cgatttttta acacaagcgg tatgaaatat
     1621 cgtgagttag ggttaaaaga caaattgcca gagatgtcag aagaagaaca atacgaactg
     1681 cttgcaaccg acggaatgtt agtaaaacgc ccactggttg taggagatga ttttgtgttg
     1741 atcgggttta aagaagcact atggaaggag acattagcat gaatcgagaa aatgcatgga
     1801 caacatatga agaagcagat atcaaggcat tagaaggact ttgcaaggaa tacaaagatt
     1861 tcttatccaa tggaaagaca gaacgtgaat gtgtagcaca gtcgatcgaa ttggcaaaga
     1921 aagctggata tcaggattta gaagaactga tcaaaaacca gacaccatta aaagcgggta
     1981 acaaagtata ccgcacatgg atggaaaaaa cgatcgcatt attccagatc ggagaagatg
     2041 cgatggaaga aggattaaat atcttaggag cacacatcga ctcaccaaga ctggatctta
     2101 aacagaatcc attatacgaa gacacagaga tggcattatt agacacccac tattacggtg
     2161 gagttaagaa atatcaatgg gttacacttc caatggcatt acacggtgtt gttgtgaaga
     2221 aagacggaac aaaagtcaat atctgtatcg gtgaaaaaga tgaagatcca gtagtgggaa
     2281 tcacagacct tttaattcat ttatcaggaa aacagatgca gaagaaagga aatgtgatcg
     2341 tagaaggaga agatcttaac gtcctgatcg gaagcaaacc attaaaaggc gaagaaaaag
     2401 aagcagtcaa gaaacagttg ttacagttat tcgatgaaga atatggaatc gaggaagaag
     2461 atttcttatc cgcagagatc gaagtcgtac cagctggaaa agcaagagac tttggattag
     2521 accgcagtat gatcatgtct tatggacaag atgacagagt ctgtgcatat acatccttag
     2581 cagctatgtt aaatgtagaa gcaccaaaga gaacatcttg ctgcatctta gtagacaaag
     2641 aagaaatagg aagtgttgga gcaacaggaa tgcactccag attctttgaa aatacaattg
     2701 cagaaatctt agctttaaca ggagattata atgatctgag gcttagaaga acgctgcaga
     2761 attcttacat gttatcatct gatgtaagtg cagctttcga tccaaactat ccaagtgtaa
     2821 tggagaagaa aaactgtgca tatttcggaa aaggattagt attaaacaaa tacactggag
     2881 ccagaggaaa atcagggtca aatgatgcaa acccagaata tatcgctcag ttaagaaaga
     2941 tctttgatga agcaaaagta gccttccaga cagctgaact tggaaaagtt gacgaaggag
     3001 gcggaggaac gatcgcctac attcctgctg cctacagcat gaacgtcatc gactgcggag
     3061 ttgcagtgtt aagcatgcat gcaccatggg agatcacaag caaatccgat atctatg
//
LOCUS       DAOMUE010000242         3111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8930_length_3111_cov_2.683246, whole genome shotgun sequence.
ACCESSION   DAOMUE010000242 DAOMUE010000000
VERSION     DAOMUE010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8930_length_3111_cov_2.683246"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..766
                     /locus_tag="PLC71_09305"
     CDS             <1..766
                     /locus_tag="PLC71_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRM02875.1"
                     /translation="EKTDFLEPLYRESKVKKVIGIVSGKGGVGKSMTTSLLGVAAMRK
                     GLHTAILDADVTGPSIPQAFGLHERLLGNAEGIILPAMTNFGIEVVSLNLMLEEETQP
                     VIWRGPVVADVVKQFWGKTLWKDIDVMFVDMPPGTGEVPLTVFQSIPLDGIVVVATPQ
                     ELVGMIVEKAVNMANMMNVPVLGLIENMSYIKCPDCGKEIKVFGDSHIEEISEKFNIP
                     VLGKLPIDPKLTQACDAGLIEDVENIYLDGVMDIIL"
     gene            1045..1446
                     /locus_tag="PLC71_09310"
     CDS             1045..1446
                     /locus_tag="PLC71_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRM02876.1"
                     /translation="MLKVMKKKLLVVIAMLVVASGFIFTGSTSTKAKDNDVKYLGKYK
                     LTAYCGCRKCSGKWGARTASGKKAKQGRTIAVDKRKIKLGSKVQINGKTYVAEDVGGG
                     VKGKHIDVFFKSHKQTKRFGKKTGVKVYKVN"
     gene            complement(1560..2471)
                     /locus_tag="PLC71_09315"
     CDS             complement(1560..2471)
                     /locus_tag="PLC71_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRM02877.1"
                     /translation="MKKPALVILAAGMGSRYGGLKQMDPMDPMGHAIIDYSIYDAKRA
                     GFGKVVFVIKKAIEKDFKETVGARVPEGMEVCYAYQEVDALPEGYNVPEGRVKPWGTA
                     HAVLCAKPFINEPFAVINADDYYGVDGYKVMADFLTSHEEKDGKAPFAMVGYHLGNTV
                     TENGYVSRGVCEVDDNHQLLSITERTHIEKREDHAEFTEDDGATWASLPFDTLVSMNF
                     FGFQPMIMDELEKGFPAFLDQAIKENPLKGEYFIPSVASDLLHDGKASLEVLVSKDQW
                     YGVTYPEDKQSVIDALAALRENGTYKF"
     gene            2750..>3111
                     /locus_tag="PLC71_09320"
     CDS             2750..>3111
                     /locus_tag="PLC71_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRM02878.1"
                     /translation="MTEIQHELIMPNKDLPFKMFLFEGRDGNYIREKHWHRSIEIFAV
                     FQGTLEFYLNDQEYLLECGDFIIVNSNEVHSIHAPKENMTVVLQMPLNMFEKYYTGDQ
                     FICFAHAPQAQDEQLMEM"
BASE COUNT         1084 a          512 c          627 g          888 t
ORIGIN      
        1 ggagaaaaca gattttcttg aaccattata tagagaaagt aaagtaaaga aggttatcgg
       61 tatcgtcagt ggaaaaggtg gcgtaggtaa atccatgaca acttctttat taggagtcgc
      121 agcaatgaga aaagggttgc atacagcaat tcttgatgcg gatgtcacag gaccatccat
      181 cccgcaggca tttggacttc atgaaagatt actaggtaat gcagaaggaa tcattcttcc
      241 agcaatgaca aatttcggaa tcgaagttgt atccttaaac ttaatgttag aagaagaaac
      301 acagccagtg atctggagag gtccagttgt agcagatgtt gtaaaacagt tctggggtaa
      361 gacattatgg aaagacattg atgtgatgtt tgtggacatg ccaccaggaa ccggagaagt
      421 tccattaaca gttttccagt ctattccatt agatggaatc gttgtcgttg caactccaca
      481 ggaattagta ggaatgatcg tggagaaagc tgtgaatatg gcgaacatga tgaatgtacc
      541 agttctgggt ctgatcgaga atatgagcta catcaaatgt ccagattgcg gcaaagagat
      601 caaggtattt ggagacagtc atattgaaga aatttctgag aagtttaaca ttccagtact
      661 cggaaaactt ccaattgatc caaaattaac acaggcatgt gacgcagggc tgatcgagga
      721 tgtggaaaat atttacttag atggtgtaat ggatatcatt ttataagaag aaacaacgta
      781 aagaagcggt tttccgcttc tttttttgat ggaaaaaatg caatgaaaaa tagaaatatt
      841 ttatacaaat atataaaaaa caaatcgtat aagttaataa atttgtaaca acaaacttac
      901 atatattgga aatgaattgt attttatcga gtacaaaggt tgacatatcg agggatatcg
      961 ggtaaagtat gaaacataga tttcgaaaat gttacaaaaa tgtttcgaaa tttaagaaac
     1021 tattgagcac agaaagaggt ttatatgctg aaagttatga agaagaaatt gttagtagtg
     1081 attgcgatgt tagtagtagc atcaggattc atttttacag gatcaacttc aaccaaagca
     1141 aaagataacg acgtaaagta cttaggaaaa tataaattaa cagcttactg cggatgcaga
     1201 aagtgcagtg gaaaatgggg agcaagaaca gcttctggaa agaaagcaaa acagggaaga
     1261 actattgctg tagataaaag aaagatcaaa ttaggatcaa aagtacagat caatggaaaa
     1321 acatacgtag ccgaagatgt cggaggcggt gtcaaaggaa aacatatcga tgtattcttt
     1381 aaatctcata aacagacaaa gagatttggt aagaaaacag gtgttaaagt ttataaggta
     1441 aattaagata cgaatgatga tcataaaata tagcgtaaaa atatcagcca gtttggaaga
     1501 aaagatgaat tcaaactggc tgatattttt ttgcttttac aaatgaatat ttaacggtat
     1561 taaaatttat aagtaccatt ttctcttaaa gcagctaaag catcaataac gctctgttta
     1621 tcttctggat atgtaacacc ataccactga tctttagata caagcacttc aagagaggct
     1681 tttccgtcat gtaacaggtc acttgcgaca ctaggaataa agtattcacc ttttaatgga
     1741 ttctctttga tcgcttgatc aaggaaagca gggaatcctt tttctaactc gtccatgatc
     1801 ataggctgga atccaaagaa gttcatggac actaaagtat caaatggaag cgatgcccaa
     1861 gttgcaccgt catcttctgt gaattctgca tgatcttcac gtttttcgat atgagttctt
     1921 tctgtgatag aaagtaactg gtggttgtca tcaacttcac agacacctct ggatacgtat
     1981 ccattctctg taactgtatt tccaagatga tatcccacca ttgcaaatgg tgcctttcca
     2041 tctttctctt catggcttgt aaggaaatca gccatgactt tatatccatc aacaccataa
     2101 tagtcatctg cgttgatcac ggcaaatggt tcattgataa atggtttcgc acataaaaca
     2161 gcatgtgcag ttccccaagg tttcacacgt ccttctggga cattataacc ttctggtaat
     2221 gcatctactt cctgatatgc ataacatact tccattcctt caggaaccct tgctccaaca
     2281 gtttctttaa aatctttctc aattgctttc tttatcacaa agactacctt accgaatccg
     2341 gcacgttttg catcatagat ggaataatca atgatcgcat gtcccatcgg atccataggg
     2401 tccatctgtt ttaatcctcc ataacggctt cccataccgg cagccagaat tactaatgct
     2461 ggttttttca tgaaaaaact cccttctgat ttttaaacta aaataagtat aacgcaagat
     2521 agaacagatg gaaagagaaa gaaaaaataa agaattggaa ttgataaaga tctatttcgg
     2581 tcagctagaa tttattgtat aatttgagta ttgaatcgtt gcaaaaaaag agaaagattt
     2641 acaaatattc gaggaggaag agtatgaaaa aacgaatatt atcagtttta ttatcgatgg
     2701 ttgtgacaaa ataaaaggat atccacgagg ataagaaaag gataaagcga tgacagagat
     2761 tcaacatgaa ctgatcatgc cgaataagga tcttccattt aagatgttcc tttttgaagg
     2821 cagagatggt aattatatca gagaaaaaca ttggcacaga tcgatcgaaa tctttgcggt
     2881 ctttcaggga acgctggagt tttatttaaa cgatcaggag tatttactgg aatgtggaga
     2941 ttttatcatt gtcaattcca acgaagtgca ttccattcat gcaccaaaag aaaatatgac
     3001 tgttgtgctc cagatgccgc ttaatatgtt tgaaaaatac tacactgggg atcagttcat
     3061 ttgtttcgcc catgccccgc aagcacagga tgaacagctc atggaaatga t
//
LOCUS       DAOMUE010000243         3098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8979_length_3098_cov_2.523497, whole genome shotgun sequence.
ACCESSION   DAOMUE010000243 DAOMUE010000000
VERSION     DAOMUE010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8979_length_3098_cov_2.523497"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PLC71_09325"
     CDS             complement(<1..1275)
                     /locus_tag="PLC71_09325"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HRM02879.1"
                     /translation="MKKNQTQIKKYAAVISIFLAAVIVGLFVFFFCIKRSVEQRSKNT
                     MRNNVVRQSEHLRTILDIQYDYLNGLAEEIGKTDHLLAKKNMNLIKSITKHTDFGGTA
                     LIKPNGDAHYDNGLIKNLSHRRYFKEVISGKRTLSDPLDSSISGSTRVVLGVPIYNSK
                     HKVIGALGGSYDVTALSRMLFEDLFNGQGCSMIIAQNGEIIAFDNFYKCCCKWIIKDK
                     VTVKKIKQDFKEGKENLVTADSKKEGTRRHYFAYMPMGVNGWILCYALPEQAAQQSYN
                     FIEDYEISFMIVFIVLVTLLILYIVYENHTRNKELLKYAQTDALTGLYNKETTEQLTD
                     ELLSEDENKYHAFLILDVDCFKQINDIYGHAVGDIVLQKFGKLLKGTFREGDILGRIG
                     GDEFGVIMKNIQTKDIAMKKAEELLAKTQAYKI"
     gene            complement(1440..>3098)
                     /locus_tag="PLC71_09330"
     CDS             complement(1440..>3098)
                     /locus_tag="PLC71_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM02880.1"
                     /translation="IVGPTILGDATTEIFKGLVRKVSGGSGIDFDKVKHILLMLLSLY
                     VASAIFSFIQGLIMTHVSNNVSYQMRKDISEKIHRMPMKYFESRTYGEVLSRVTNDVD
                     TLGQSLNQSMTQIITSATQIVGVFIMMIRISIPMTIAVLLTLPLSMGLIGLIMSKSQP
                     YFKAQQKHLGELNGQVEEIYSGHNIVKAFNKEESVIEEFKEVNGKLYNSAWRSQFFSG
                     AMMPLMQFVGNLGYVAVTILGGYLAIKKTIEVGQIQSFLQYVRNFSQPITQLAQVGNM
                     MQTTAASAERVFEFLEEEEEIEVEKDAVPVDTLAGNVTFEHVNFGYNKDQTIINDFSV
                     DVKEGQKVAIVGPTGAGKTTLIKLLMRFYDVNGGCIKVDGHDIREFKRSDLREMFGMV
                     LQDTWLYNASIRDNIRYGKLDASEEEIQGAARAAYAHHFIETQPGGYDMEINEESNNI
                     SQGQKQLLTIARAILADPKILILDEATSSVDTRTEERIQMAMDNLMQGRTSFVIAHRL
                     STIKDADVILVMNHGDIVEQGNHEELLAKGGFYADLYNSQFENA"
BASE COUNT          825 a          683 c          466 g         1124 t
ORIGIN      
        1 gatcttgtat gcttgtgttt tagccagcaa ttcttctgct ttcttcattg ctatatcctt
       61 tgtctggata ttcttcataa tgactccgaa ttcatcccca ccgatccttc ctaatatatc
      121 tccttcacgg aaagtgcctt ttagaagttt cccgaatttc tgtaacacga tatccccgac
      181 tgcatgtcca tagatatcgt tgatctgctt aaaacagtcc acatccaaaa tcaaaaatgc
      241 atggtactta ttctcatctt ctgataaaag ttcatccgtt aactgctcgg ttgtttcttt
      301 gttatataat cctgtcaatg cgtcagtttg tgcatatttc agtaattctt tgttccttgt
      361 atgattctca taaacaatat acaggatcaa taaggtcacc aaaacaataa atacgatcat
      421 aaagcttatt tcgtagtctt caatgaagtt atatgactgt tgtgctgcct gttctggtaa
      481 tgcataacag agtatccaac cattgacccc cattggcata tatgcaaagt aatgtcttct
      541 tgtcccttct ttcttgctgt ctgccgttac aagattttct tttccttctt taaaatcctg
      601 tttgatcttc ttcactgtta ctttgtcttt gatgatccat ttgcaacaac atttatagaa
      661 attatcaaat gcaatgattt ctccgttttg cgcaatgatc atactgcatc cttgtccatt
      721 aaaaagatct tcaaacagca tacgacttag agctgttacg tcataagaac ctccaagagc
      781 tccgatcact ttgtgtttgc tgttatatat aggaactcct aacacaactc ttgttgatcc
      841 agaaatacta ctatctaatg gatcacttaa tgttcttttt ccactgatca cttctttaaa
      901 atatcttcga tgcgataaat tctttatcaa tccattgtca taatgagcat ccccatttgg
      961 cttgatcaat gcagtcccac caaaatctgt atgttttgtg atcgacttga tcaaattcat
     1021 attcttcttt gctaacagat ggtctgtctt gccaatctct tctgctaatc catttaagta
     1081 atcatattga atatctaata ttgttcttaa atgctcactc tgtctgacca cgttgttcct
     1141 catcgtgttt tttgatcttt gttcaacaga acgttttatg caaaagaaga aaacaaaaag
     1201 tcctacgatg actgccgcta agaatatact gatcacagcc gcatattttt taatctgtgt
     1261 ctgattcttc ttcataactt tgattctcct tgattctata tctggatata tttttgttct
     1321 atttaactat accaaaattt gctatatccg tctatttaat cctgtgaaaa tcttttctat
     1381 attagaataa aaaaagagaa aactttcgtt ttcccttttt tcacaatgtt ttattattct
     1441 taagcgtttt caaactggct attataaaga tccgcataga atccaccctt ggccagcaat
     1501 tcttcatgat ttccctgttc cacaatgtct ccgtgattca taactaagat cacgtcggca
     1561 tctttgatcg ttgataagcg atgggcgatc acaaaacttg ttcttccttg catcagatta
     1621 tccattgcca tctgaattcg ttcttctgtt cttgtatcga ccgaacttgt tgcttcatca
     1681 aggatcaaaa tctttggatc tgctaagatt gctcttgcga tcgttaataa ctgtttctgt
     1741 ccttgggaga tattattgct ttcttcgttg atctccatat catatccacc tggctgtgtt
     1801 tcaatgaaat gatgtgcgta tgcagctctt gctgctcctt ggatttcttc ttcgcttgca
     1861 tctaatttac catagcggat attgtcacgg atacttgcat tgtaaagcca tgtatcctga
     1921 agcaccatac caaacatttc tcttaggtca cttctcttga attctctgat atcatggcca
     1981 tctactttaa tgcagcctcc attcacatca tagaaacgca tcagaagttt gatcaacgtt
     2041 gtctttccag ctccagttgg tcctacgatc gcaactttct gaccttcttt gacatcgacg
     2101 gagaagtcat tgatgatcgt ttgatctttg ttatatccga agtttacatg ttcaaatgtt
     2161 acatttccag caagtgtatc aacaggtacc gcgtcttttt caacttcgat ttcttcctct
     2221 tcttccagga attcaaagac acgttctgca gatgctgcgg ttgtctgcat catgttacct
     2281 acctgtgcta actgtgtgat cggctggctg aagtttctta catactgtaa gaatgactgg
     2341 atctgaccta cctcgatcgt cttcttgatt gctaaatatc cacctaatat tgttactgct
     2401 acataaccta aattcccaac aaactgcatc agcggcatca ttgctcctga gaagaattgt
     2461 gatctccatg cagaattgta aagttttcca tttacttctt taaattcttc gatcacgctt
     2521 tcttctttgt taaatgcttt gacaatgtta tgtccactat agatttcttc tacctgtcca
     2581 tttaactctc caagatgttt ctgctgtgcc ttgaaatatg gctgagattt tgacatgatc
     2641 aatccgatca atcccataga taatggaagt gtcaataaaa ctgcgatcgt catcggaata
     2701 ctgatacgga tcatcataat aaatacaccg acgatctgtg tcgctgatgt gatgatctgt
     2761 gtcatactct ggtttaagct ctgccctaat gtatcaacat catttgtaac tcttgacaat
     2821 acttcaccat aggttctgct ttcaaaatat ttcattggca tacgatgaat cttctctgaa
     2881 atatcttttc tcatctggta actgacattg tttgatacat gtgtcatgat cagtccctga
     2941 ataaatgaga aaatcgcact tgctacatat aagcttaaca acatcaataa aatatgttta
     3001 actttatcaa aatcaattcc acttccacca gatactttgc gaaccagtcc tttgaagatc
     3061 tcagttgttg catctcctaa gatcgttggt ccaacaat
//
LOCUS       DAOMUE010000244         3098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_8981_length_3098_cov_2.124877, whole genome shotgun sequence.
ACCESSION   DAOMUE010000244 DAOMUE010000000
VERSION     DAOMUE010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8981_length_3098_cov_2.124877"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="PLC71_09335"
     CDS             complement(<1..1219)
                     /locus_tag="PLC71_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRM02881.1"
                     /translation="MKQFEEWEGFKGRIWKEEINTRDFIQENYTPYDGDESFLAGSTE
                     ATDKLWGILQGLQKEERKKGGVLDMDTDIVSSLTSHGPGYISEETKDLEQIVGLQTDK
                     PLKRAFMPYGGIKMAEESCRNYGYEPSERLHEIFTKYSKTHNTAVFDAYTPEMKKARH
                     NKIITGLPDTYGRGRIVGDYRRVALYGIDYLLEEKENDFANCGCGVMTDDVIRLREEI
                     AEQKKALKGMKEMAQIYGFDISRPAKTAKEAIQWMYFGYLAAIKTQNGAAMSIGRVAT
                     FLDIYIQRDLDKGIITEEEAQEMIDHLTMKFRMVKFARIPSYNQLFSGDPVWATLDLA
                     GIGVDGRSMVTKTDFRFLHTLENMGPSPEPNITVLYSSDLPENFKKYAADISVLTSSI
                     QYENDDVMRPVWGD"
     gene            complement(1473..2651)
                     /locus_tag="PLC71_09340"
     CDS             complement(1473..2651)
                     /locus_tag="PLC71_09340"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HRM02882.1"
                     /translation="MKELELKYGCNPNQKPSRIYMADGSELPIEVLNGRPGYINFLDA
                     FNGWQLVRELKKATGLPAATSFKHVSPAGASVGRPMSDTLKKIYWVDDMGDLSPLACA
                     YARARGADRMSSFGDFISLSDVCDADTARLIKREVSDGVIAPGYTDEALEILKAKKNG
                     NYNIIKIDENYTPAPLEHKQVFGVTFEQGRQELPIDDELLSNIVTENKEIPEEALIDM
                     KISLIVLKYTQSNSVCFVKDGQAIGIGAGQQSRVHCTRLAGQKADNWWLRQCPKVMNL
                     PFKEKIRRADRDNAIDLYIGDEYMDVLADGAWENIFTEKPEVFTKEEKREWLDKLTDV
                     TLGSDAFFPFFDNIERAHKSGVKYIAQPGGSVRDDAVIDCCNKYNMAMAFTGIRLFHH
                     "
     gene            complement(2684..>3098)
                     /locus_tag="PLC71_09345"
     CDS             complement(2684..>3098)
                     /locus_tag="PLC71_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HRM02883.1"
                     /translation="KGLTFEQSLRSREFEPDGPNYTPRISGLMKIKDGTFHYAMSILK
                     SNNGNPESCNRFTYTYENPIAGEGRFIHTYMHDGNPLPSFEGEPKLVAIEEDIDDFTN
                     TLWNSLNDDNKVSLFVRYIDIATGEYETRIVNKNK"
BASE COUNT          822 a          666 c          504 g         1106 t
ORIGIN      
        1 catcgcccca taccggacgc ataacatcat cgttctcata ctggatagaa cttgtcagta
       61 cggagatgtc agctgcatat ttcttgaagt tttctggaag atcagaagaa tataatacag
      121 tgatgtttgg ttctggagat ggtcccatgt tctctaatgt atgtaagaaa cggaagtctg
      181 tctttgtaac catggaacgt ccatccacac cgataccagc aaggtcaagt gttgcccata
      241 ctgggtctcc tgagaataac tggttgtaag aaggaattct tgcgaatttt accattctga
      301 atttcattgt taaatgatcg atcatttcct gagcttcttc ttctgtgatg attcctttgt
      361 caaggtcacg ctggatatag atatcaagga atgtagcgac acgtccgata ctcatggcag
      421 ctccattctg agttttgatc gctgctaagt atccaaagta catccactgg attgcttctt
      481 ttgcagtttt cgctggtctt gaaatatcaa atccatagat ctgagccatt tctttcatac
      541 cttttaatgc ttttttctgt tctgcaatct cttcacgaag acggatcaca tcatctgtca
      601 tcacaccgca tccacagttt gcaaaatcat tttctttttc ttctaataag tagtcaattc
      661 cgtataaagc aacacgacga taatcaccta caatacgacc acgtccataa gtatctggaa
      721 gacctgtgat gatcttgtta tgtcttgctt ttttcatttc tggtgtgtaa gcatcgaaaa
      781 cagctgtgtt atgagtcttt gaatattttg taaagatttc atgtaatctt tcacttggtt
      841 cataaccata gttacgacaa gattcttctg ccatcttgat accaccatat ggcataaatg
      901 cacgttttaa tggtttgtct gtctgaagac caacgatctg ctccaaatct tttgtttctt
      961 cactgatata accaggtcca tgagaagtta aagaagatac gatatctgta tccatgtcaa
     1021 gaactccacc tttttttcgt tcttctttct gaagtccctg taagataccc catagcttat
     1081 ctgttgcttc tgtagatcct gcaaggaagc tttcgtctcc atcataaggt gtgtagtttt
     1141 cctggatgaa atctcttgta ttgatttctt ccttccagat tctaccttta aatccttccc
     1201 attcttcaaa ctgtttcatt ttgaaaactc ctccatttct tttggattaa aattattata
     1261 aaaactcctt tggttagctc tatctaactc aattacatta tagcaaaaat aaaattgaat
     1321 acaatatact taaccgataa actttctcgt taaataaata acaactcctg tttttcatat
     1381 ttttttgcat acaaaaacag tatatggtcc aaaaaattat acttcagacc atatactgtt
     1441 tttcatgttc aatgactata taaagaaact ttttagtgat ggaataatct gattcctgta
     1501 aatgccattg ccatgttgta cttattacag cagtcaatga cagcgtcatc tcttacagat
     1561 ccacctggct gtgcgatgta ttttacgcca ctcttatgtg ctctctcaat gttatcaaag
     1621 aatgggaaga atgcatcaga tcccaatgta acatctgtta acttatctaa ccattctctc
     1681 ttttcttcct ttgtaaagac ctctggcttc tcagtgaaga tgttttccca tgctccgtct
     1741 gctaaaacat ccatgtattc atctccaatg taaagatcaa tcgcattatc tctgtctgca
     1801 cggcggattt tttctttaaa tggcaggttc ataacttttg gacactgtct taaccaccag
     1861 ttgtctgctt tctgtcctgc aagtcttgta caatgcacac gtgactgctg ccctgctccg
     1921 attccgatcg cctgtccatc ttttacaaaa catacagagt tagactgtgt gtattttaat
     1981 acgatcagag aaatcttcat atctatcaat gcttcttctg gaatctcttt gttctcagtc
     2041 acgatattag ataataattc atcatcgatc ggaagttcct gtcttccctg ttcaaatgta
     2101 acaccaaata cctgtttatg ctctaatggt gctggtgtat agttctcatc aatcttaatg
     2161 atgttatagt ttccgttttt ctttgctttt aagatttcca gtgcttcatc tgtgtatcct
     2221 ggtgcgatca ctccgtcaga tacttctctc ttgatcaaac gtgctgtatc tgcatcacat
     2281 acatcagata aagaaataaa gtcaccgaat gaagacatac ggtctgcacc tcttgctctg
     2341 gcatatgcac atgctaatgg agataaatct cccatatcat ctacccagta gatcttcttt
     2401 aaggtatcag acattggacg tcctacagat gctcccgcag gtgatacgtg tttgaaagaa
     2461 gttgcagcag gaagtcctgt tgctttcttt aattctctta ccagctgcca tccattaaat
     2521 gcatccaaaa agttgatgta ccctggtctt ccgtttaata cttcaattgg aagttcactt
     2581 ccgtctgcca tgtagattct tgatggtttc tggtttggat tacatccgta ctttaattct
     2641 aattccttca tttttcttct cctttgatct gctgatcgac tgattatttg ttcttattta
     2701 cgattcttgt ctcatattct cctgttgcaa tatcaatata acgtacaaac agagatactt
     2761 tgttgtcatc gtttaagctg ttccaaagtg tatttgtgaa gtcatcgata tcttcttcga
     2821 tcgctactaa tttaggttct ccttcaaaac ttggaagtgg attcccatca tgcatatatg
     2881 tgtggataaa acgtccttct cctgcgattg gattttcata tgtatatgta aatcgattgc
     2941 aactttctgg atttccattg ttactcttta aaatagacat tgcatagtga aatgtcccat
     3001 ctttaatctt catcagacca gaaattcttg gtgtatagtt tggtccgtct ggttcaaact
     3061 cacgacttct taaagactgc tcaaaagtaa gtcctttt
//
LOCUS       DAOMUE010000245         3080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9047_length_3080_cov_2.111074, whole genome shotgun sequence.
ACCESSION   DAOMUE010000245 DAOMUE010000000
VERSION     DAOMUE010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9047_length_3080_cov_2.111074"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..1519
                     /gene="yqeC"
                     /locus_tag="PLC71_09350"
     CDS             107..1519
                     /gene="yqeC"
                     /locus_tag="PLC71_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HRM02884.1"
                     /translation="MIIAVAGSGGKTTRVHKLAQYYRSLGKKVFVTTTTHMKKESDTV
                     IPENIEDIRKQLNETGYCMAGMPATPENALVQKIGPLPEDFYETAVKEADITLIEADG
                     SRGMPAKIPADYEPVIPENIDEIHIVIGMSALGKPASKAVHRLSLADKDLEIKEDTIL
                     TPLHLQKLLKKGYLGPLREQYKDTKIKVYPGQADTLYQRVIARFLQEEKDVAQIKDDW
                     FKIQPKLVIFGAGHVAIQLLRIAKFLDFYTIMIDDREEFADPEKLSQADEVYCRDFHD
                     IEDILPEQDNAFYVVVTRGHANDRLCAETVLRRPYLYLGMIGSKGKVAKTFEIMKEEG
                     YSEEQISTIHAPIGLKIGARTPEEIAISIAAEMIAIKNHETESTMSKELFETKESGVL
                     CIITKKSGSSPRGVGSMMLVTKDGIIGSIGGGNLEKTVMEEAPSVKEITRKKYDLSNA
                     QSATLGMICGGKNEILYVPV"
     gene            1551..2741
                     /locus_tag="PLC71_09355"
     CDS             1551..2741
                     /locus_tag="PLC71_09355"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HRM02885.1"
                     /translation="MGKYNFDEVIDRHGTDCLKYDFGMKRKGRDDLLPLWVADMDFRL
                     PDEILDEFHKRIDHGIFGYTDPLDEYFDAMNHWFSTRYGYTIKPEWVTLGAGIVYALG
                     TSVKAFTEEGDAMMVMQPVYYPFSEVIKNDGRKLVNCQLRYENNRYSIDFEKMEQMIK
                     EENVKALIFCNPHNPVGRVWTREEMEEVAEICLKYNVIWMVDEMHCDFIFPGHEFTSC
                     MNLDKKFHEIIALYSSPGKTFNVAGLQPANIIIPNEELRKKYQWANTQAAYSQGSLMG
                     QLAVKVCYTKGADWVDELVEYIYENVKYMSRYVKENFPKAKMVEPEGTYLVWVDFSGY
                     GFSNEELEHLMLEEAKLWLDSGIIFGPETAQFERFNVACPRVTVEQALTQLKNALDKH
                     LAEK"
     gene            2785..>3080
                     /locus_tag="PLC71_09360"
     CDS             2785..>3080
                     /locus_tag="PLC71_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02886.1"
                     /translation="MEKQKKNLGSVAFLPLIVFLVCYVGCGITFTLMGSDDPFGKFPR
                     HVALLAGCAVALLLNRSMDVKDKLNIMTTNMGNSGVMMIVLIYLMAGGFQGAAAA"
BASE COUNT         1130 a          462 c          713 g          775 t
ORIGIN      
        1 cagggaatgg taagattcag ttttggacat gagacaacga tgaaagaaat caaccagata
       61 ataaatgcag ttaagattct tgcagaagaa taaagaggaa aaagatatga tcattgcagt
      121 tgcaggttcc ggtggaaaaa caacgagagt tcataagtta gcacagtatt atagaagtct
      181 tggaaagaaa gtctttgtca caacgacaac tcatatgaaa aaggaatccg ataccgtgat
      241 ccctgaaaat atagaagata ttcgaaagca attaaatgaa accggatatt gtatggcggg
      301 aatgcctgcg acgccagaaa atgcattagt acaaaaaatc ggaccattac cagaagattt
      361 ctatgaaact gcagtaaaag aagcagatat cacgctgata gaagccgatg gttccagggg
      421 aatgccggca aagattccag cggattatga accagtgatc cctgaaaata tcgatgagat
      481 tcatattgtg atcggaatgt cagcacttgg gaaaccagcc agcaaggctg ttcacagact
      541 ttcattagcg gataaggatt tagagattaa agaagatacg atccttacac cgttgcattt
      601 acaaaaatta ctgaagaaag gatatctagg accattgaga gagcaatata aagatacaaa
      661 aatcaaagta tatccaggac aggcagatac actatatcaa agagtgatcg ctcgattctt
      721 acaggaagaa aaagatgtcg cacagatcaa ggacgattgg tttaagattc agccgaaatt
      781 agttatcttc ggggctggac atgtagcgat ccagttactc cggattgcga aatttttaga
      841 tttctacacg attatgatcg atgacagaga ggaatttgca gatccagaga aactatcaca
      901 agcagatgaa gtttactgca gagattttca tgacatagaa gatattttgc cagaacaaga
      961 taacgcattt tatgtggttg tcacacgtgg acatgcaaat gacagattat gtgcagaaac
     1021 ggtattacga agaccatatc tctatcttgg aatgatcgga agcaaaggaa aagttgcaaa
     1081 aaccttcgag ataatgaaag aagaaggata cagtgaagaa cagatcagca cgatccatgc
     1141 accaatcgga ttaaagatcg gtgcaagaac gccagaagag attgcaatca gtatcgcagc
     1201 agagatgatc gcaatcaaaa atcacgaaac agaaagtacg atgagtaaag aactttttga
     1261 gacaaaagaa tcaggagtgc tgtgtattat cacaaagaaa tccggttcat ctccacgagg
     1321 tgttggaagt atgatgcttg tcacaaaaga tggaattatt ggaagtattg gtggaggaaa
     1381 cttagaaaaa actgttatgg aagaagcacc atcggtgaaa gagatcacaa ggaaaaaata
     1441 tgatctatcg aatgcacaga gtgcaacact tggcatgatc tgtggtggta aaaatgaaat
     1501 attatatgtt ccagtataaa aagacagaaa aaacaacaca ggagtagaaa atgggaaaat
     1561 acaattttga cgaagtaatc gacagacatg gaacagattg tttgaaatac gattttggaa
     1621 tgaagagaaa aggaagagat gacctgcttc ctctttgggt agcagatatg gattttcgtc
     1681 tgccagatga gattcttgat gaatttcata aaagaattga tcatgggatt tttggatata
     1741 cggatccctt agatgaatat tttgatgcaa tgaaccactg gttttcaaca agatacggat
     1801 atacgatcaa accagaatgg gtaacacttg gagcaggaat cgtttatgca cttggaacaa
     1861 gtgttaaggc atttaccgaa gaaggagatg ccatgatggt tatgcagcca gtgtactatc
     1921 catttagtga agttatcaaa aatgatggaa gaaaactggt aaactgtcag cttcgttatg
     1981 aaaacaatcg ttattccatt gattttgaaa aaatggaaca aatgatcaaa gaagaaaatg
     2041 taaaagcact gatcttctgc aacccacaca atccggtagg aagagtctgg acaagagaag
     2101 aaatggaaga agtcgcagaa atctgtttaa aatacaacgt aatctggatg gttgatgaga
     2161 tgcattgtga ttttattttt ccaggacatg agtttacaag ctgtatgaat ctggataaaa
     2221 aatttcatga gatcattgca ctttacagct cgccaggaaa gacatttaat gttgcaggat
     2281 tacagccagc aaatatcatt attccaaacg aggaattaag aaaaaaatat caatgggcta
     2341 atacacaggc agcttacagt cagggaagct taatgggaca gcttgctgtg aaagtatgtt
     2401 acacaaaagg tgctgactgg gtagatgaat tggtagaata tatatatgaa aacgtaaagt
     2461 atatgagccg atatgtaaaa gagaactttc caaaagcaaa gatggtagaa ccagaaggga
     2521 cttatttagt atgggttgat ttctcaggat atggattcag caatgaagaa ttagaacatt
     2581 taatgttaga agaagcaaaa ttgtggctgg attctggaat catctttgga ccagagacag
     2641 cacagtttga gcgtttcaac gttgcgtgtc cacgagtgac ggttgagcag gcactgacac
     2701 agttaaaaaa tgcactggat aagcatctgg cagagaaata agacagattt atctggatat
     2761 atattaagat tgagaggaaa agaaatggag aaacaaaaga aaaatctagg atctgtggcc
     2821 tttcttccac tgatcgtatt tctagtatgt tatgtaggat gtggaattac atttaccttg
     2881 atgggatcag acgacccatt tggaaaattt ccacgtcatg tcgcactttt ggcaggatgt
     2941 gcggtagcac ttcttttaaa tagaagtatg gatgtaaaag ataaattaaa tattatgacg
     3001 acaaacatgg gaaattctgg agttatgatg attgttttga tttatctgat ggcaggggga
     3061 tttcagggag cagcagcggc
//
LOCUS       DAOMUE010000246         3078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9058_length_3078_cov_2.358915, whole genome shotgun sequence.
ACCESSION   DAOMUE010000246 DAOMUE010000000
VERSION     DAOMUE010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9058_length_3078_cov_2.358915"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..>3078
                     /locus_tag="PLC71_09365"
     CDS             157..>3078
                     /locus_tag="PLC71_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM02887.1"
                     /translation="MSDTAKRKKPNMIHIGIGILTVLLVVFIVILMQLVSNIQGTARV
                     VNYAGLIRGETQRIIKLENSGQREDELIKEVQSFIEGLRHGNDQLKLVRLKDDDFQDK
                     MKELDEYFISLHKEIYRVRTVGYEHTDIISKSERFFKICDEATGLAEVYAQRKATSLA
                     TLEKFITADIVVLMLLIGYEFIKAVRFAAMNRILRHKAYLDNATGLPNKNKCEEILGE
                     LNSPEDIIVCSFDLNNLRRINDSMGHDAGDAYIYRFAVCLRSSIPSEHFVGRLGGDEF
                     IAVLQRLDKQAVRKLFEDLRKDIAAESKEHREIPLSYAAGFAMASDYPGKTMRELFND
                     ADKNMYINKNHVKREEALEEKRLNYQLLKLLNQHGKNFLDCLYCDAKLDTYRVIRSSE
                     NFFLALDGAYSSAVEQIVQEKIGKDEQKNIWERLQITRLQEKMKEKQDVQEYQYDMQE
                     EGFYKRMTLIPVDWDEDKKLHHFLLAFEIIRKGADNQTGAREQLQIYYEQLKQSIIEN
                     DSYVDALLELSDVIYTVNLKTDILERNIILKGKEQKNKELFLDYPLPCNYQDYCAEYE
                     KKVTKETKAEYNMTNDTKKLLERYNNGDTNISVEYCFHEDDGSIRWVQKTILMTQIVV
                     YDEETMSEIPTVYAITLLQDTSQRHKQGELEHARLQAEFDEMRTASQAKTDFLSRMSH
                     DIRTPLNGIIGLLNINEKHFDDQKLVLENHKKMETAAKHLLSLINDVLQMSKIEEGQI
                     TLAHQEIAVKKLMREVETMIRERVLEAGIRWNYEEDNIDDRYSYVYASPLHLRQILLN
                     IYGNCIKYNHPGGTISTKMEQMDVHDKICTYRWTIKDTGIGISKDYIDHIFEPFSQEK
                     TDARSVYQGTGLGMSIVKGLVEQMHGEIKVTSEKGMGSTFVITIPFEIAQKKEQPVAE
                     KIVEQADIRGLNLLVAEDNQLNAEIIQTLLTDDGANVTVVEDGQQAVDQFKNASQGTF
                     DA"
BASE COUNT         1080 a          470 c          730 g          798 t
ORIGIN      
        1 aaaaaggatt ccaaaaagat agaacagatt tttggagatt atctctggaa cacgatcagt
       61 ataagggata cagcagttgc caaagaaaat ttcatcaagt acgggattgc atgttataag
      121 aaattttcta aagtagtgat aggggaataa aagtttatgt ctgatacggc aaaaagaaaa
      181 aaaccaaata tgatccatat cggcattggt attctgacgg tacttttagt ggtctttatc
      241 gtcatcctga tgcagttggt ctcgaatatt cagggaacag cccgcgttgt aaattatgcg
      301 gggttaataa ggggagaaac acaaaggatc attaagctag agaattccgg acagcgagaa
      361 gatgagctga tcaaggaagt gcagtccttt atcgaagggc tgcgtcatgg caatgaccaa
      421 ttaaaactcg tacgattaaa ggatgatgat tttcaggata agatgaagga actggatgaa
      481 tattttattt ccttacacaa agaaatttat cgtgtgcgga cggtcggcta tgagcataca
      541 gatatcattt caaagagcga gagattcttt aagatctgtg atgaggcaac aggtcttgcg
      601 gaagtatatg cacagagaaa agcaacgtca ttagcaactc ttgagaaatt catcacagca
      661 gatatcgttg tattgatgct gctgatcgga tatgagttta ttaaagcggt ccgttttgcg
      721 gcgatgaacc gtatattgcg gcataaagct tatttagata atgcaacagg acttccgaat
      781 aagaataaat gtgaagagat attaggtgaa ctgaattcac cagaggatat tattgtatgc
      841 agttttgacc ttaataatct ccgtcggatc aacgatagta tgggacatga tgcaggagat
      901 gcgtatattt atcggtttgc tgtttgtctg cgatcttcca taccatcgga acattttgtc
      961 ggaagacttg ggggagacga atttatcgca gtgctgcagc ggcttgataa acaagcggtg
     1021 agaaagctat ttgaagatct tcgcaaagat atagcagcag aatccaaaga acatcgagag
     1081 attccgttaa gttatgcagc gggttttgct atggcaagtg attatcctgg caaaacaatg
     1141 agagaattat tcaatgatgc agataaaaat atgtatatca ataaaaacca tgtgaaacgg
     1201 gaagaagcac ttgaagagaa gcggttgaat tatcaattat taaaactgct gaatcaacat
     1261 ggaaagaact ttttagactg tttatattgt gatgctaagc tggatacata tcgtgtgatc
     1321 cgttccagtg aaaacttttt cttagcttta gatggtgcgt attccagtgc agtagagcag
     1381 atcgtacagg aaaagattgg gaaagatgag cagaagaaca tttgggaaag gcttcagatc
     1441 acaaggcttc aagagaagat gaaggagaaa caggatgtac aggaatatca atatgatatg
     1501 caggaagaag gattctataa gcgtatgaca ttgatcccag ttgactggga tgaagataag
     1561 aaattacatc attttcttct ggcatttgaa atcatccgaa aaggagctga caatcagaca
     1621 ggggccagag aacagttaca gatttattat gaacagctaa aacaatcaat cattgaaaat
     1681 gacagttatg tcgatgcact tttggaatta tccgatgtga tctatacggt caatctaaag
     1741 acagatattc tggagagaaa tattattcta aagggaaaag agcaaaagaa caaagagctg
     1801 tttcttgatt atccattacc atgtaattat caggattact gtgcagaata tgagaaaaaa
     1861 gtcacgaagg agacaaaagc agagtataat atgacaaatg atacgaaaaa actgctggag
     1921 agatacaaca atggtgacac aaatatttct gtggaatatt gttttcatga agatgatgga
     1981 agtattcgct gggtacaaaa aacaattctt atgacacaga tcgtggtata cgatgaagag
     2041 acaatgagcg agattccgac agtttatgcg atcacgctct tacaggatac atcgcaaaga
     2101 cataaacaag gagaattaga acacgcacgt ttacaggcag aatttgacga aatgcgtaca
     2161 gcaagtcagg cgaagaccga tttcttaagc cgtatgagcc atgatatcag aacaccatta
     2221 aatggaatca ttggacttct aaatatcaat gaaaaacatt tcgatgatca gaaactggta
     2281 ttagagaatc ataagaaaat ggaaactgct gccaaacatc tgctttctct gatcaacgat
     2341 gtattgcaga tgagtaagat cgaggaaggt cagatcacac tggcacatca agagattgct
     2401 gtgaagaaac tgatgcggga agttgagacg atgatcagag agcgggttct agaggcagga
     2461 atccgatgga actatgaaga agataacata gatgacagat attcatatgt atatgcaagc
     2521 ccattgcatc ttcgtcagat cttattgaat atttatggaa actgtattaa atataatcat
     2581 ccgggtggaa ctatttctac aaagatggaa caaatggatg ttcatgataa gatctgtacg
     2641 tatcgttgga cgatcaaaga cacgggaatt ggtatcagta aagattatat cgatcatatt
     2701 tttgagccat tttcacagga aaagacggat gcccgaagtg tatatcaggg gactgggctt
     2761 ggaatgtcga tcgtcaaggg actggtagaa cagatgcatg gagaaatcaa agtgacaagc
     2821 gagaaaggaa tgggatctac ctttgtgatc acgattccat ttgaaatcgc acagaagaaa
     2881 gaacagccag tggctgagaa gatagtggag caagcagata ttcgtggttt aaacttatta
     2941 gttgccgagg ataatcaatt gaatgcagaa atcatacaga cacttctgac ggatgatgga
     3001 gccaatgtca ctgttgtgga agatgggcaa caggcagtgg atcaatttaa gaatgcgtca
     3061 caaggaacat ttgatgcg
//
LOCUS       DAOMUE010000247         3078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9059_length_3078_cov_2.243467, whole genome shotgun sequence.
ACCESSION   DAOMUE010000247 DAOMUE010000000
VERSION     DAOMUE010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9059_length_3078_cov_2.243467"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PLC71_09370"
     CDS             complement(<1..954)
                     /locus_tag="PLC71_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009531150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRM02888.1"
                     /translation="MCKDVYVFAEQRDGNLQKVGIELIGEARKLADDLGQNVVAVLLG
                     NQIKDKASELIAHGADKVVVVDDEMLAEYVTEPYAKAMMEIIQSHNPEIVLYGATSIG
                     RDLAPRVSARIHTGLTADCTKLDIDEETKLLMMTRPAFGGNIMATIVCEDFRPQMATV
                     RPGVMKALEADESRQGEVEVVEVNFTDADMNVKIREVVKTTHKSVDITEAKILVAGGR
                     GVGNAEGFKDLEALADTLDGEVAASRAAVDSGWISNDRQVGQTGKTVRPELYLACGIS
                     GAIQHAAGMENSEFIVAINRDEDAAIFDIADLGIVGDIKKIL"
     gene            complement(974..1771)
                     /locus_tag="PLC71_09375"
     CDS             complement(974..1771)
                     /locus_tag="PLC71_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRM02889.1"
                     /translation="MKIVVCAKQVPDTTEVKLDPKTNTLIRDGVPSIINPDDKAGIEA
                     ALQLKEKCPGSTVTVVSMGPPQADVALREALAMGCDDALLISDRAFGGADTWATSSTI
                     AAALKKLDYDVIITGRQAIDGDTAQVGPQIAEHLGIPQVSYVEKMDVEGEDTLVVQRQ
                     FEDRHQIIEVKTPCLLTALAELADPRYMSVHGVFDAYREKEVKVWGLEDIKDTVNEGN
                     IGLKGSPTRVKKSFTKQAKGAGTILNDLSDDEAVAAIVEKLQEKHII"
     gene            complement(1791..2927)
                     /locus_tag="PLC71_09380"
     CDS             complement(1791..2927)
                     /locus_tag="PLC71_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HRM02890.1"
                     /translation="MVEFKLTKEQLLAKELFRSFAENEIKPISRDMDEAEKFDMDLLD
                     KMKKYGLMGIPYSKEYGGEGADVLTYALAMEEVSKVDASAGITISVHTSLCCSCINDY
                     ASEEQKQKFLRPLVDGSKIGCFGLTEPGAGTDASGVKTTAKLDGDHYVLNGQKVFTTN
                     SGFADTFIIFAYTDKSAGTRGMSAFIVDRDSEGLTVSKDIPRMGIRAASNAEVALVDV
                     KVPKENLIGREGMGYKIAMGALAGGRIGIGAQSVGIAQGALNEAIKYVKERKQFGKPI
                     AKFQNTQFLIAALQTKIDAARLMVWRAAKAKDDHENFAPLAAQCKLFASDVAVEVTRG
                     AVQMLGGYGYSREYPVERMYRDAKIIYEGTSEAMKMIVGGGLLR"
BASE COUNT          767 a          740 c          470 g         1101 t
ORIGIN      
        1 aagaatcttc ttaatatcac ctacgatacc aaggtctgcg atatcaaaga tcgctgcatc
       61 ttcatctcgg ttgatcgcta cgatgaattc tgaattctcc atacctgctg cgtgctggat
      121 cgctccagaa attccacatg ctaagtataa ctctggacga accgtcttac ctgtctgtcc
      181 aacctgacga tcattgctga tccatcctga atctacagct gctcttgatg ctgctacttc
      241 accatctaat gtgtctgcta atgcttcaag atctttaaat ccttctgcat ttcctacacc
      301 acgtcctcct gctaccagga tcttagcttc tgtgatatct acagatttgt gcgttgtttt
      361 tacaacttca cggatcttaa cattcatatc tgcatctgta aagtttactt caacaacttc
      421 aacctcaccc tgtctgcttt catcagcttc taatgctttc ataacaccag gtcttactgt
      481 tgccatctgt ggtctgaaat cttcgcagac gatcgtagcc atgatatttc caccaaatgc
      541 tggtcgtgtc atcattaata attttgtttc ttcatcaata tcaagttttg tacagtctgc
      601 tgttaatcct gtgtggattc ttgcagaaac tcttggtgca agatctcgtc cgatcgatgt
      661 tgctccatag agaacaatct ctggattatg ggactgaatg atctccatca ttgcttttgc
      721 atatggttct gtcacatatt ctgccagcat ttcatcatct actactacaa ctttatctgc
      781 tccatgagcg atcagttcac ttgctttgtc tttaatctga tttccaagta aaactgcaac
      841 tacattctgt ccaagatcat ctgcaagctt tcttgcttct ccgatcagtt cgataccgac
      901 tttctgaaga tttccatctc tctgctcagc aaatacataa acatctttac acattttgat
      961 tgtcctcctt accttaaatg atgtgttttt cctgtaattt ttctacgatt gcggcaactg
     1021 cttcgtcatc ggagagatca tttaagatcg ttcctgcacc ttttgcctgt tttgtaaagg
     1081 atttcttaac tctggtagga gaacctttta atccgatatt tccttcattg actgtatctt
     1141 tgatatcttc taatccccat actttaactt ctttttcacg atatgcatca aatacaccgt
     1201 gaacagacat atatcttgga tcagcaagtt cagctaatgc tgttaaaaga caaggtgtct
     1261 tcacctcgat gatctggtga cgatcttcaa actgtctctg gacaactaaa gtatcttccc
     1321 cttctacatc catcttctct acataactta cctgagggat tccaaggtgt tctgcgatct
     1381 gcggtccaac ctgcgctgta tctccatcga ttgcctgacg acctgtgatg atcacatcat
     1441 agtctaattt ctttaatgct gctgcaattg tagaagaagt agcccatgta tctgctcctc
     1501 caaatgcacg atctgagatc aataatgcat catcgcatcc cattgctaat gcttctctta
     1561 atgcaacatc tgcctgaggt ggtcccatag aaacaacagt tactgtactt cctgggcatt
     1621 tttcttttaa ctgaagtgct gcttcaattc ctgctttatc atctgggtta ataatacttg
     1681 gaacaccatc acggatcagt gtatttgtct ttggatctaa ttttacttcc gttgtatctg
     1741 gtacttgttt tgcacaaaca acaatcttca ttctcttgtc tccttctttc ttatcttaat
     1801 aatccaccgc caacgatcat cttcatagct tcggatgtac cttcataaat gatctttgca
     1861 tcacgataca ttctctctac tggatattct cttgagtatc catatccacc aagcatctga
     1921 actgctcctc ttgtaacttc aacagctaca tcagaagcaa ataatttaca ctgtgctgca
     1981 agtggtgcaa aattttcatg atcatctttt gctttcgctg ctctccatac cattaatctt
     2041 gctgcatcaa tctttgtctg taatgctgcg atcaagaact gtgtattctg gaattttgcg
     2101 attggttttc caaactgttt tctttctttt acgtatttga tcgcttcatt taatgcaccc
     2161 tgtgcaatac caacagactg agcaccgatt ccgatacgtc ctcctgctaa agctcccata
     2221 gcgatcttat atcccattcc ttctcttccg atcaggtttt cttttggaac tttcacatca
     2281 actaatgcta cttctgcgtt ggatgctgct ctgattccca tacgaggaat atctttgctg
     2341 actgttaatc cttcgctgtc tctatctacg ataaatgcag acatacctct tgttcctgca
     2401 ctcttatctg tgtaagcaaa aataatgaat gtatctgcaa atccagagtt tgttgtaaat
     2461 actttctgac catttaatac ataatgatca ccgtctaatt ttgcagttgt tttaactccg
     2521 ctggcatctg ttccagctcc tggttctgtc agtccaaaac atccaatctt acttccgtca
     2581 actaatggac gtaagaattt ctgtttctgt tcttctgatg catagtcgtt aatacaagaa
     2641 cagcaaagag aagtatgtac ggaaattgtg attcctgcac tggcatcaac tttggatacc
     2701 tcttccattg ctaaagcata tgttaataca tctgctccct ctccaccgta ttctttagaa
     2761 tatggaattc ccatcagacc gtatttcttc atcttatcta acagatccat atcaaatttc
     2821 tctgcttcat ccatatctct ggaaattggt ttgatctcat tctcagcgaa acttctgaac
     2881 aattcctttg caagcagttg ttctttcgtt aatttaaatt caaccattct ttttatcctc
     2941 ctgtctggtc aaaataattt gcctgttata ttcttcgtat gttttatttt tcgtgtacca
     3001 atactttgta cgctaattat atgactaaaa aaatatgttg tcaaggattt aacaaatatt
     3061 ttttcatgta tactgtgt
//
LOCUS       DAOMUE010000248         3053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9174_length_3053_cov_2.254169, whole genome shotgun sequence.
ACCESSION   DAOMUE010000248 DAOMUE010000000
VERSION     DAOMUE010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9174_length_3053_cov_2.254169"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..>772)
                     /locus_tag="PLC71_09385"
     CDS             complement(11..>772)
                     /locus_tag="PLC71_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRM02891.1"
                     /translation="KKQVMAEIIRHTSLSDGIFDMTLKAEEIAKEAKAGQFISVYLNN
                     KSKILPRPISICGIDKEAGTLRIVYRTVGDGTKELSDYKEGEMVKILGPLGNGFTQKD
                     KKAILIGGGIGIPPMLELMKQLDCDKTAVLGYRDSDMFLKDEFEAVGDVVISTEDGSF
                     GTKGNVIDAIKEQGVEGSIIYACGPTPMLRGIKAYAEEMGIEAQISMEERMACGIGAC
                     LACVCKSKDVDSHSHVHNKRVCKDGPVFDAREVEL"
     assembly_gap    773..782
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(824..1747)
                     /gene="pyrF"
                     /locus_tag="PLC71_09390"
     CDS             complement(824..1747)
                     /gene="pyrF"
                     /locus_tag="PLC71_09390"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009264467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRM02892.1"
                     /translation="MINQLVNNIKKLDAPIVVGLDPMLDYVPEYIKKAAFAEYGETLE
                     GAAEAIWQYNKGIIDATYDLIPAVKPQIAMYEQFGVPGLVAFNKTCEYAKSKGLVIIG
                     DIKRGDIGSTSKAYAIGHVGKVAVGSKTYSGFCEDFVTVNPYLGSDGIRPFMEVCKEE
                     KKGMFILVKTSNPSSGEFQDQEIDGKPLYEMVAEKVASWGEELMEDSYSYIGAVVGAT
                     YPKMGEVLRKIMPKSYILVPGYGAQGGQAKDLAPFFNEDGLGAIINSSRGIICAYKQD
                     AYSQFGEENYADASRQAVLDMKEDLKQAWVK"
     gene            complement(1890..2549)
                     /locus_tag="PLC71_09395"
     CDS             complement(1890..2549)
                     /locus_tag="PLC71_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRM02893.1"
                     /translation="MEYQGIIFDFNGTLFFDNDKHVKAWNEISKILRGKEITLEELHT
                     KLNGTPNIQNILYFTDGKATKEELEKYSQKKEEYYRQFCKEDTEKFHLVDGVCEFFDH
                     LKEKDIPFTIASASIKENIDFFIESFGLDRWIKPEDIVYDDGTYENKIAMFHKAADVI
                     GIDMKDIRIYEDSNSGIRNAYDAGCEQIVVICDKENEEEFLKRPGVIEVMQDFSFAKE
                     L"
     gene            complement(2572..>3053)
                     /locus_tag="PLC71_09400"
     CDS             complement(2572..>3053)
                     /locus_tag="PLC71_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRM02894.1"
                     /translation="SKKPPLQNQGFKWWEHVTEIWAVATNIYIEGTFPNGVQYDMASA
                     IQLMHNMMVAHAKAVIAYKEAGYEGKIGIVHSLESKYPYDETKDEDVKAAKNEDVLNN
                     QFLLDATFLGEYRDETMEIINRLVELNNGSFHASKDDMEILKEAAYWYREVSKTKEL"
BASE COUNT          802 a          713 c          429 g         1099 t
ORIGIN      
        1 gataaattca ttatagttcc acctccctgg cgtcgaatac tggtccatct ttgcaaacac
       61 gtttgttatg aacatgtgaa tgggaatcca cgtcttttga cttgcaaaca caggctaagc
      121 atgcaccgat tccacatgcc attctctctt ccatggaaat ctgtgcttcg atccccatct
      181 cttctgcata tgctttgatt cctcgaagca ttggtgttgg accacatgca tagatgatgc
      241 ttccttccac gccctgctct ttgatcgcat caatgacatt tcccttcgtt ccaaaagatc
      301 cgtcttctgt agagatcaca acatctccaa ctgcctcaaa ttcgtctttt aagaacatat
      361 cactgtcacg gtatcctaaa actgctgttt tgtcgcaatc taactgcttc attaattcta
      421 acatcggagg aattccgatt ccgccaccga ttaaaattgc ttttttgtct ttctgtgtaa
      481 atccgtttcc taaaggtcct aagattttga ccatctcacc ttctttgtaa tcggataatt
      541 ccttcgttcc atctccaacc gtacgataga cgatccttaa tgtccctgct tctttatcga
      601 tcccacagat actgatcggt cttggaagga tcttactctt gttattcaga taaacagaaa
      661 taaactgtcc tgcttttgct tctttcgcaa tctcttctgc tttcaatgtc atatcaaaga
      721 ttccgtctga taaagatgta tgacgaatga tctctgccat tacctgtttc ttnnnnnnnn
      781 nntttatctg gctgttttct ttactcacaa aataggtgtt cttttatttt acccaggcct
      841 gttttaaatc ttctttcata tcgagaacag cctgtcttga tgcatctgcg taattttctt
      901 ctccaaactg actgtaagca tcctgtttat atgcacagat gattcctctg gaagaattaa
      961 tgatcgcacc taatccatct tcgttaaaga atggcgcaag atcctttgcc tgaccaccct
     1021 gtgctccgta tcctggaact aagatgtatg attttggcat gatctttctt aatacttctc
     1081 ccatcttagg ataagttgct ccaacaacgg caccgatata gctgtaagaa tcttccatca
     1141 gttcttctcc ccaggaagca actttctcgg ctaccatctc atataacggt tttccatcga
     1201 tctcctgatc ctggaattct ccgctggatg ggtttgatgt cttaactaag atgaacattc
     1261 ccttcttctc ttctttacat acttccataa atggacggat tccatcactt ccaagatatg
     1321 gattaactgt tacaaaatct tcacaaaatc cgctgtatgt cttgcttccc actgcaacct
     1381 ttcctacatg accgatcgca taagctttag atgttgatcc aatatctcca cgtttgatat
     1441 ctccgatgat cacaagacct ttgctctttg catattcaca tgtcttatta aatgcaacaa
     1501 gtcctggaac tccaaattgt tcatacatag caatctgtgg tttaactgct ggaatcagat
     1561 cataagttgc atcaatgatt cctttattat actgccagat tgcctctgct gctccttcta
     1621 aagtctcacc atattcagca aatgctgctt tcttaatata ttctggtaca taatcaagca
     1681 ttggatctaa tccaacaaca ataggggcat ctaatttttt aatattgttt actaactgat
     1741 tgatcatcga actctccttt atatctttct tcttatattt caaaagatta taccctttct
     1801 catttattga ttaaggtata ttcaatttat cttatcacaa aaagggagtt tcgtctatga
     1861 aactccctaa aatcaatttt aaatttgtat tacaattctt ttgcaaagga aaaatcctgc
     1921 ataacttcta taactcctgg acgttttaaa aattcttctt cattttcctt gtcacagatc
     1981 acaacgatct gttcacatcc tgcatcatat gcatttcgga ttccagaatt ggaatcttca
     2041 taaattctaa tgtctttcat atcaatgccg atcacatcgg ctgctttatg aaacattgcg
     2101 atcttattct catatgttcc atcatcataa acgatatctt ctggcttgat ccagcggtcc
     2161 agtccaaagg actcaataaa gaaatcaata ttttctttga ttgatgcact agcaatcgta
     2221 aatggaatat ctttttcttt caaatgatca aaaaactcac atacaccatc taccagatga
     2281 aatttttccg tatcttcttt acagaattga cgataatatt cttctttttt ctgagaatat
     2341 ttttctaatt cttcttttgt tgccttccca tctgtaaaat ataaaatatt ttgtatattt
     2401 ggtgttccgt ttagtttcgt atgtaattcc tctaacgtga tttcttttcc tcgtaagatc
     2461 ttagagatct cattccacgc tttcacatgt ttatcattgt caaaaaataa tgttccattg
     2521 aaatcaaaaa tgattccctg atattccatt tctcattcct ccattgcaat tttacagttc
     2581 ttttgtcttg cttacttcgc gataccagta tgcagcttct tttaagatct ccatatcatc
     2641 tttgcttgca tgaaagcttc cgttgtttaa ctctaccaaa cgattgatga tctccattgt
     2701 ttcatccctg tattccccaa ggaatgttgc atctaacaag aactgattat ttaatacatc
     2761 ttcatttttt gccgctttaa catcttcgtc tttcgtttca tcataaggat atttactttc
     2821 taacgaatga acgataccta tcttcccttc atagcctgct tctttgtatg caataacagc
     2881 ttttgcatga gccaccatca tattgtgcat taactggatc gcacttgcca tatcatattg
     2941 tacaccattc ggaaaggttc cttcaatata tatattggtt gcaaccgccc agatctcggt
     3001 aacatgctcc caccacttaa atccctgatt ttgaagtggg ggcttcttgc tca
//
LOCUS       DAOMUE010000249         3045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9208_length_3045_cov_1.957860, whole genome shotgun sequence.
ACCESSION   DAOMUE010000249 DAOMUE010000000
VERSION     DAOMUE010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9208_length_3045_cov_1.957860"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="PLC71_09405"
     CDS             <1..654
                     /locus_tag="PLC71_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HRM02895.1"
                     /translation="SFFSPGQGASLEFYQKMEKVYQKGGYKLLGYADQGFRVMSTNKN
                     VKSINDFKGQKIRTMENSYHLKFWKTLGANPTPMTFSEVYIGLQQGTIDAQENPYEVI
                     VSSKLYEQQDYVVETNHLPHYISLIVSDEFYNSLSKDQQKIIDQASQTAKVYAREASD
                     KRIADRIKTIEDSGTKIVKLDAKTQKEMRKQARPVYDAIKKSISKDIYNAYLSGVEK"
     gene            786..1286
                     /locus_tag="PLC71_09410"
     CDS             786..1286
                     /locus_tag="PLC71_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HRM02896.1"
                     /translation="MFQKFYPSEDIRSTYDIDFERYYKEGYRGVIFDVDNTLVPHNAP
                     ADERAKKLFKRLDAMGMQYCFTSNNKEPRVKAFCEAVGGKYYVYKANKPSIKGYETAM
                     KLMGTDKKNTFFVGDQLFTDVYGANRTGIHSILVTPMNPKEEIQIVIKRYFEKIVLWQ
                     YRKKKH"
     gene            complement(1398..>3045)
                     /locus_tag="PLC71_09415"
     CDS             complement(1398..>3045)
                     /locus_tag="PLC71_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM02897.1"
                     /translation="NGKITNIQPVSASDIPSRVGGDIPRALTTDEIYTIVDKFAEAAK
                     RAVTAGFDAVEIQAGHGYLLNQFLSPTTNDRTDEFGGSKENRARFTTLVLKAVRETVG
                     ENIPIFLGISADELAEGGNTLDDTLEIVEYFKDYVDVFDVSAGLTCSIQYQYDADYLP
                     DGWKSYMSKSIKEKTGKPCQTSGNIREPEVANRILEDGEADLIAIGRGLIADPEWVNK
                     VQFGNTDDINRCISCNNGCTATLGDEPIRCSINPALVSGSLYKDKKLKKPCNVVVVGG
                     GTAGLEAACTAAEVGCATVLIEKSGELGGLARRVGKMPNKQRLGYFPQYLERRAMKLK
                     NLFICKNTEASIEFIEGFKPDIVVNATGSVPLLPNIPGLKENLEAGNVSTIFDLVDHV
                     DSYPEDLSGKKVVVIGGGNVGLDVVEFFVPRKADVTIVEQKAHFGENLDFITKTGSIE
                     FMNKNKVNQYPNASLLEVKDHSFIVRHDYKDLELDFDYGFVCLGMQSATPSLQELYNH
                     FIYEGVEVLNIGDSAQTRRIIEGVKEGRDILVALERQDFI"
BASE COUNT         1006 a          564 c          588 g          887 t
ORIGIN      
        1 tcgttttttt ctccagggca gggggcatcc cttgagttct atcagaagat ggagaaagta
       61 tatcagaagg gtggatacaa attacttgga tatgccgatc agggattccg tgttatgtct
      121 accaataaga atgttaagag tattaacgac ttcaaaggac agaagatccg tacgatggag
      181 aacagctatc atttgaagtt ctggaagaca cttggagcga atccaacacc aatgacgttc
      241 agcgaagttt atatcggact tcagcaggga acgatcgatg cacaggagaa tccttacgaa
      301 gttatcgttt ccagcaaatt atatgaacag caggattatg ttgtagagac aaaccatttg
      361 ccacattata tttcgctgat cgtcagtgat gaattctata acagtctgtc aaaagaccag
      421 cagaagatca tcgatcaggc atcgcagaca gcgaaagtgt atgcaagaga agcatctgac
      481 aagagaatcg cagaccgtat caaaaccatt gaagacagtg gtacaaagat tgtgaaactt
      541 gatgcaaaga cacagaaaga gatgcgtaag caggcacggc cagtttatga tgcgattaag
      601 aagagtatct caaaagatat ttataatgca tatttaagtg gtgtagagaa ataaaatgaa
      661 tgatatgaga ctagaatttt tgaaaaaact ggtctcatat tttgttttct tcttgctaat
      721 tgtaaaaagc tgttataaaa taaacaagat tgaacaatgt aaagatgtaa aaaggaagat
      781 aaattatgtt tcagaaattc tacccgtccg aagatatcag aagtacttat gatattgatt
      841 tcgaacgata ctacaaagaa ggatatcgag gagtgatctt tgatgtcgat aatacgttag
      901 taccacacaa tgcaccggca gatgaacgtg caaagaagtt atttaagaga ctggatgcga
      961 tggggatgca gtattgtttt acaagtaaca acaaagaacc aagagtgaaa gcgttctgcg
     1021 aggcagtcgg tgggaaatat tatgtataca aagcaaataa accatcgatc aaaggatatg
     1081 agacagcaat gaaattgatg ggaacggata agaagaatac attcttcgtt ggagatcagt
     1141 tgtttacaga tgtttatgga gcgaacagaa caggtataca tagtattctt gtaacaccga
     1201 tgaatccgaa agaagagatt cagatcgtca taaagcgtta ttttgaaaag attgtgttgt
     1261 ggcagtaccg taagaaaaaa cattaatgaa ataataaaaa acatgggtca gatctttgtt
     1321 atctggtcca tgttttttat tattttacaa tatatttctt atcttttaaa gaaaggtttg
     1381 aaatcaaaat tatgatctta gatgaaatcc tgacgttcta atgcaactaa aatgtcacga
     1441 ccttctttta caccttcgat gatacgacgt gtctgagcac tgtcaccgat attaagtact
     1501 tcaacacctt catagatgaa gtggttgtat aattcctgaa gagaaggagt tgcagactgc
     1561 attccaagac atacaaatcc ataatcgaag tctaattcaa gatctttata gtcatgtctt
     1621 acaataaagc tgtgatcttt aacttctaat aaagaagcat ttggatactg atttacttta
     1681 ttcttattca tgaattcgat agaaccagtc ttagtgataa agtcaaggtt ctcaccgaag
     1741 tgagctttct gctcaacgat cgttacatca gcttttctag gaacaaagaa ttcaacaacg
     1801 tcaagtccta cgttaccacc accgatcaca acaactttct taccagataa atcttctgga
     1861 taagaatcta cgtgatctac cagatcaaag atcgtagata cattacctgc ttctaagttc
     1921 tcctttaatc caggaatatt tggaagaaga ggaacggatc ctgttgcgtt aacaacgata
     1981 tctggcttga aaccttcgat aaattcgata gaagcttctg tattcttaca gataaacagg
     2041 ttcttaagtt tcattgcacg acgttctaag tactgaggga aatatccaag acgctgtttg
     2101 ttaggcatct ttcctacacg tctggcaagt ccaccgagtt ctcctgattt ctcgatcaga
     2161 acagttgcac atccaacttc agcggctgtg catgcagctt caagaccagc agtaccacca
     2221 ccaacaacga caacgttaca tggtttcttt aatttcttat ctttatataa tgaacctgaa
     2281 acaagagcag gattgatgct gcatctgata ggttcatccc ctaatgttgc tgtacatcca
     2341 ttgttacatg agatacaacg attgatatca tctgtgttac caaactgaac tttatttacc
     2401 cattcaggat cagcgatcag tccacgtccg attgcgatca gatctgcttc accatcttca
     2461 agaatacggt ttgcaacttc tggttcacgg atattaccac ttgtctgaca aggctttcca
     2521 gtcttttctt tgattgattt agacatataa gatttccatc catctggaag gtaatctgca
     2581 tcatactggt actggataga acatgtaagt ccagcagaaa catcaaatac atcaacgtaa
     2641 tctttgaaat attcaacgat ctctaatgta tcatctaatg tgttaccacc ttcagcaagt
     2701 tcatcagcac tgattcctaa gaagatagga atattttcac caactgtctc acgaacagct
     2761 tttaatacta atgtagtgaa acgtgcacga ttctctttgg atccaccaaa ttcatcagta
     2821 cggtcatttg ttgttggaga taagaactgg tttaataaat aaccatgtcc agcctgaatc
     2881 tcaacagcat caaatcctgc tgtaactgca cgttttgctg cttcagcaaa tttatcaacg
     2941 atcgtgtaga tttcatctgt tgttaaagca cgaggaatat caccaccaac tctggatgga
     3001 atatcagatg cagatactgg ttggatgttt gttatcttac cattc
//
LOCUS       DAOMUE010000250         3022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9301_length_3022_cov_2.126727, whole genome shotgun sequence.
ACCESSION   DAOMUE010000250 DAOMUE010000000
VERSION     DAOMUE010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9301_length_3022_cov_2.126727"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1696)
                     /gene="htpG"
                     /locus_tag="PLC71_09420"
                     /pseudo
     CDS             complement(1..1696)
                     /gene="htpG"
                     /locus_tag="PLC71_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520349.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
     gene            1925..2557
                     /locus_tag="PLC71_09425"
     CDS             1925..2557
                     /locus_tag="PLC71_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HRM02898.1"
                     /translation="MNKINYQKQLDKVIENLGETKPTLLLHSCCAPCSSYVMEYLSQY
                     FDITIDYYNPNIDSKEEYEKRVHEQQRLVDEMNLPIKVIDAGYAPQTFFDMAKGYEQE
                     PEGGKRCYRCYKLRLDQAAKLAQEGGYDYFTTTLTISPLKNATWLNELGQKAGELAGV
                     RFLPSDFKKKGGYQRSIQLSKEYNLYRQNFCGCVFSKRESEERMHKEKNI"
     gene            2831..>3022
                     /locus_tag="PLC71_09430"
     CDS             2831..>3022
                     /locus_tag="PLC71_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HRM02899.1"
                     /translation="MNAFLILEDGHVFEGKSFGAQKEVICEVVFNTSMTGYLEVLTDP
                     SYQGQGVVMTYPLIGNYGVC"
BASE COUNT          981 a          504 c          533 g         1004 t
ORIGIN      
        1 aatctttgca tcttttaatt tttcaacgtg aacttcaaga ttttctttgt ttaaagtctt
       61 tctaaacagg tcagttaaag aagtcttgat ttcatctaat tctttttcat ctacatcttc
      121 tttaagggca tcttcgacat ccgcatcgat acgtttaaat ttgatatgct gattctgctg
      181 ttcgatctgt gtgataaatg cagaatcaat gttatgattt aaaatgacag catccatatc
      241 ctgttctttg aacatattga tatactggct ttgctgaact tcatctgtga tatagtataa
      301 agttgtcaca tcatcatcgt ttgcaggagc aggaacaagg tcacttaaag ttacatattt
      361 atgttccata ttcttaaata agatgtaatc tttgatctta tctgcgaatt tctggtcacg
      421 gatgaatcca aatttgataa atggactgat atcatcccag tatttttcat aagattcacg
      481 gtctgtcttg cacataccag ttaacttatc agcaactttc tttgtgatgt aatcagaaat
      541 tttcttcacg aatccatcgt tctgaagagc acttcttgat acgttcagtg gaaggtcagg
      601 acaatcgatc acacctttta ataacattaa gaattcagga atgacttctt tgatgttatc
      661 ggcgataaat acctgattgt tatataattt aattgttcct tcgatagaat catattctgt
      721 attgatctga gggaagtaaa ggataccttt taagttaaat gggtaatcca tgtttaggtg
      781 gatccagaat aatggttctt taaagtcatg gaagactttg tgatagaagt ttttgtattc
      841 ttcatcagta cattcatttg gacgtttatt ccaaagagga tgtgtatcac ttaaagaaac
      901 aggtcgttta ttgatcttaa caagatcttt tgcaggggaa acttctatag tttcttttgt
      961 accgtcttcg ttttcttttt cttcagtttt tgcatcttca tggatgtgtt ctacaacgac
     1021 atcatcgtct tttacctgat cttctgggat tgtctcaaat tcctgttcgg caccttctac
     1081 agataagaaa atctctgttg gcatgaaaga gcagtatttc tcgatgatct ctcttgcacg
     1141 gtattcattt gcgaattctg tgctttcttc atttaagaat aaagtgatct ctgtaccaac
     1201 cgtttctttt gtacctgttg ccattgtgta ttctgtacca ccatcacaag tccagtgaac
     1261 aggttctgct ccttctttat aagaaagagt gtcaatcgta acttcgtccg ctaccataaa
     1321 tgcagaatag aaaccaagac caaaatgtcc gatgatctgg tcaccttctg ttttgtcttt
     1381 gtactgttct aagaactgag ttgcaccaga aaatgcaatc tgagtgatgt atttttcaac
     1441 ttcatcagca gtcataccaa gaccagtatc gatgaatttt aaagttttct tctcaggatc
     1501 aaggataact tcaactttgt ttttatgtcc ttctgggaaa ttgtattctc ccatcatatc
     1561 taatttctgt gatggcatca catccgttag agattaattc acggaagaaa atatcgtgat
     1621 cggaataaag ccatttctta atgattggga aaatattatc gctgttaatg gaaagagttc
     1681 cctttgtttc tgccataatt tgtacactcc tttatctaaa aatattatga tcttaatcaa
     1741 gtgttctatg ccaaatagaa cttttacaaa gagtattgta gaaccaaagt tgataaaagt
     1801 caagtaaaaa ttagcactca tgaaaaaaga gtgctaacaa ttaactgccg gtgttttctt
     1861 tttatataaa tactagtata atagaaagaa aacagtaaaa aacaaaatca gaaagagaca
     1921 accaatgaac aaaataaact accaaaaaca attagataaa gtaatcgaaa acttaggaga
     1981 aaccaaacca accctattac tccacagttg ctgtgcgcca tgtagcagct atgtcatgga
     2041 atatctctcc caatattttg atatcaccat tgactactac aatccaaata tcgactccaa
     2101 agaagagtat gaaaaaagag tccacgaaca acagcgtctg gtcgatgaaa tgaatcttcc
     2161 tattaaagtg atcgatgctg gatatgcccc gcagactttt tttgatatgg caaagggcta
     2221 tgaacaggaa ccagaaggtg gtaagagatg ttatcgttgt tacaaattaa gattggatca
     2281 ggcggcaaaa cttgcccaag aaggtggata tgattatttc acaacaaccc ttacgattag
     2341 tccattaaag aatgctacat ggctgaatga acttggacag aaagcaggag aattagcagg
     2401 agtcagattt cttccatctg attttaaaaa gaaaggtggt tatcagagat ctattcaatt
     2461 atcgaaagaa tataatttgt atcgtcagaa tttctgcgga tgtgtttttt ctaaaagaga
     2521 gagtgaagag cggatgcata aagaaaaaaa catataaaaa tacaaaaaag tacttgcaac
     2581 gacaaaaaat atatgataaa atttttacaa tttgtagaga aatatttctg aaataaaaaa
     2641 taacttgggt attggaagga caagatcgct gaatttccaa tacttagctt tgtaattttt
     2701 attttagggt atttcttttt tttatataaa atcaagggtt ttggcaaaac tcttatttta
     2761 taaataggaa gttagttctt cctgacaaaa tacaacccta tgaaagagac tttttaggag
     2821 gaaaaattac atgaacgcat ttttgatatt ggaagacgga cacgtatttg agggaaaaag
     2881 ttttggagcc caaaaagaag tgatctgtga agtcgtattt aatacttcca tgacaggcta
     2941 tttagaagta ttaacagatc cttcctatca gggacaggga gtcgtaatga cttacccact
     3001 gataggaaat tatggagtat gt
//
LOCUS       DAOMUE010000251         3015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9320_length_3015_cov_2.183108, whole genome shotgun sequence.
ACCESSION   DAOMUE010000251 DAOMUE010000000
VERSION     DAOMUE010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9320_length_3015_cov_2.183108"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="PLC71_09435"
     CDS             <1..1264
                     /locus_tag="PLC71_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRM02900.1"
                     /translation="RDVAIAAMLGAEEFGFATAPLVTLGCVMMRVCNLDTCPVGVATQ
                     NPELRKKFAGKPEYVINFMKFIAQELREYMAKLGVATVDELVGRTDLLKPNAKAAEKH
                     VDLSRILGYKYDPAQKMDYNHKNVYDFKLEQTADMKVLMKELMPALEKKQKKSIQLDV
                     TNIDRTFGTIFGSEITKHWGEQALEDDTYVVKCNGAGGQSFGAFIPKGLTLQLVGDSN
                     DYFGKGLSGGKLILCPPTGIKFKAEDNIIVGNVALYGATSGKAFINGVAGERFCVRNS
                     GATAVVEGVGDHGCEYMTGGRVLVLGPTGKNFAAGMSGGVAYVLDEDSKLYKNLNKEM
                     VSVETVTSKYDVIELKDMLKEHVAYTNSEKGKEILKNFEQYLPKFKKIMPNDYKAMLN
                     LIVQMEERGLSSEQAKMEAFNIKANAGK"
     gene            1274..2761
                     /locus_tag="PLC71_09440"
     CDS             1274..2761
                     /locus_tag="PLC71_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRM02901.1"
                     /translation="MGKPTGFLDYEREDARAESPKERIKHFNEFHIPLSREEQQKQGA
                     RCMDCGVPFCQSGKVLLNGMASGCPLNNLVPEWNDLIYTGNWEQAYLRLKKTNPFPEF
                     TGRVCPALCENACTCGLNGNPVCSKQNELSIIEYGYKHGLAKARPPKFRTGKKVAVIG
                     SGPSGLSAANSLNKRGHEVTVYERSDRLGGLLMYGIPNMKLEKHIVERKIAIMKEEGI
                     HFVTNANVGKDIKPEQLKKDYDAVVLACGSSNPRNIEVPGREAKGIYFAVDFLKSTTK
                     SLLDSGLKDKKYISAKGKNVIVIGGGDTGNDCVGTSIRHGAKSVTQLEMMPKLPDERA
                     ENNPWPEWPRICKTDYGQEEAIAVFGHDPRIYQTTVKEFKMNKKGEVTKAVLVNLESK
                     KDEKTGRMMMVPVEGSEKEIPADLVLIAAGFLGSEAYVTEAFGVKVNQRTNVETKPDS
                     FATNVENVFTAGDMHRGQSLVVWAIREGRDAAREVDKQLMGYTNL"
BASE COUNT         1092 a          457 c          687 g          779 t
ORIGIN      
        1 acgtgatgtg gcgattgcag caatgcttgg agcggaagaa tttggctttg caacagcacc
       61 acttgtaaca cttggatgtg ttatgatgag agtatgtaac ttagatacat gcccagtcgg
      121 agttgcaaca cagaatccag aacttcgtaa aaaattcgca ggaaaacctg aatatgttat
      181 aaacttcatg aagtttattg ctcaggaatt aagagaatac atggcgaaat taggagtagc
      241 tacagttgat gaattagtcg gacgtacaga cttattaaaa ccaaatgcaa aagcggctga
      301 aaaacatgta gatctttcaa ggattcttgg atataaatat gatccagcac agaagatgga
      361 ttacaaccac aagaatgtat atgatttcaa actggagcag acagcagata tgaaagtgct
      421 gatgaaagaa ttaatgccag cactcgaaaa gaaacagaag aagagtattc agcttgatgt
      481 tacaaatatt gaccgtactt tcggaacgat ctttggatca gagatcacaa agcactgggg
      541 tgaacaggca ttagaagatg acacatatgt tgtaaaatgt aacggagctg gtggacagag
      601 tttcggagcc ttcattccaa aaggtctgac attacagtta gtcggagaca gcaatgacta
      661 cttcggaaaa ggactttctg gcggtaaatt aatcctttgc cctccaacag gaatcaaatt
      721 caaagcagaa gataacatca tcgtaggtaa cgttgcatta tacggagcta caagcggtaa
      781 agcatttatc aatggtgttg ctggagaacg tttctgtgtc agaaactcag gagctacagc
      841 ggtcgttgag ggtgttggag atcacggatg tgaatatatg acaggtggtc gtgttcttgt
      901 tcttggacca acaggaaaga acttcgcagc tggtatgagt ggtggagttg cttatgtatt
      961 agatgaagac agtaagttat ataagaactt aaataaagaa atggtttcag tagaaactgt
     1021 aacatccaaa tatgacgtta ttgaattaaa agatatgtta aaagaacatg ttgcatatac
     1081 aaattccgaa aaaggaaaag aaattctaaa aaactttgaa cagtatctgc ctaaatttaa
     1141 gaaaatcatg ccaaatgatt acaaagcaat gttaaatctg atcgttcaga tggaagaacg
     1201 tggattaagc agtgaacagg caaagatgga agcatttaat atcaaggcaa atgcaggaaa
     1261 ataggaggaa attatgggaa aaccaactgg atttttagat tatgaaagag aagacgcccg
     1321 tgcagaatct ccgaaagaac gaatcaaaca ttttaacgaa tttcatattc ctctttcaag
     1381 agaagaacag cagaaacagg gtgcaagatg tatggactgt ggagtaccat tctgtcagtc
     1441 aggaaaagta ttattgaatg gaatggcatc tggttgccca cttaacaact tggtgccaga
     1501 gtggaatgac ttgatctata caggaaactg ggaacaggca tacttaagac ttaagaaaac
     1561 aaatccattt ccagagttta ccggacgtgt atgtccggca ctctgtgaga atgcctgtac
     1621 atgtggatta aatgggaatc ctgtatgttc caaacaaaat gaattaagta tcatcgagta
     1681 tggatacaaa catggattag ctaaagccag accaccgaaa ttccgtacag gaaagaaagt
     1741 tgcagtgatc ggttctggac cttctggatt atctgcagcc aactctttaa acaaacgtgg
     1801 acatgaagtt acagtgtacg aacgttctga ccgtcttggc ggattgttaa tgtatggaat
     1861 tccaaatatg aaattggaaa aacatatcgt agaacgtaag attgcgatca tgaaagaaga
     1921 aggaattcac tttgtgacta atgcaaacgt tggaaaagat attaaaccag aacagttaaa
     1981 gaaagattac gatgcagtag ttttagcttg tggatcatca aatccaagaa atatcgaagt
     2041 tcctggacgt gaagcaaaag gaatttattt tgcggttgat ttcttaaaat ccacaacaaa
     2101 gagtctgtta gattctggat taaaagataa gaaatatatc tctgccaaag gaaagaatgt
     2161 tattgttatc ggtggtggag atacaggaaa tgactgcgtt ggtacatcca ttcgtcatgg
     2221 agcgaagagt gttactcagc tagagatgat gccaaaactt ccagatgaac gtgcagagaa
     2281 caatccatgg ccagaatggc caagaatctg taaaacagat tatggacagg aagaagcgat
     2341 cgcggttttt ggacatgatc cacgtatcta ccagacaaca gtcaaagaat tcaaaatgaa
     2401 taaaaaaggc gaagtcacaa aagctgtttt agtaaatctt gaaagcaaaa aagatgaaaa
     2461 gacaggaaga atgatgatgg ttccagtgga aggatctgaa aaagaaattc cagcagatct
     2521 tgtactgatc gcagcaggat tcttaggaag tgaagcttat gtcacagaag cttttggggt
     2581 taaagttaac cagagaacaa atgtagaaac aaaaccagat agttttgcga caaatgtaga
     2641 aaatgtcttt actgcaggag atatgcatag aggacagtca cttgttgtat gggcgatccg
     2701 tgaaggaaga gatgcagctc gtgaagttga caaacaatta atgggatata caaatcttta
     2761 agttataaaa attgaaaaat gatgtcagaa atacaaatat cgtctgacat catttttcgt
     2821 ttgtaaaaaa caaaactatt tttgataagt tttcgagaaa gtcttttcaa aagagattgt
     2881 ttttgctatg ataatccgtg gagaaaaatt cttgataaaa gagagaagta tgagggagca
     2941 gattggatta tgcattttat tggtcacttt aaaacgatta caagacataa gatcctggtg
     3001 gcaaaaggct gtttt
//
LOCUS       DAOMUE010000252         3011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9340_length_3011_cov_2.586604, whole genome shotgun sequence.
ACCESSION   DAOMUE010000252 DAOMUE010000000
VERSION     DAOMUE010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9340_length_3011_cov_2.586604"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLC71_09445"
     CDS             complement(<1..770)
                     /locus_tag="PLC71_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM02902.1"
                     /translation="MRTMKKVLAAGLTAVMAMSLMVGCGSKKDDSSSKTYNLGIIQFA
                     EHGSLDNCRKGFLKGLESEGIKEGENLKITYKNSQADTATDNQIASNFASKKLDMICA
                     IATPSAQSAYNAAKDSDIPVVYTAVTSPKAAGFVDKEGKNVGNITGTSDLVLADKQLK
                     LIRQMMPKAKNVGIFYSTNEANSKAGIEAYEKVADKYGFKIITQGITASADMPMAADS
                     LLKKVDCITNLTDNLVVSNMQTYLQKANKLNIPVFGSEV"
     misc_binding    complement(842..1080)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1128..1769)
                     /locus_tag="PLC71_09450"
     CDS             complement(1128..1769)
                     /locus_tag="PLC71_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRM02903.1"
                     /translation="MTLKKKIEIAVILCSIGICSFGQNVFAGEFLRLPAQTNAKVEQV
                     VQTRLLEEQRAYLMQAQLMTKVNLEQQIKDKPVYIPKTKHVVTQRERSILERIVEAEA
                     TDKDEKSKILVANVILNRVRSKEFPNSIESVVFQRAYGKVQFSPTADGRYESVRITKS
                     TKRSVKKALEDGIDYSEGALYFVEKTMANPKNVSWFDEALTRLFTYQGHSFYK"
     gene            complement(1908..2597)
                     /locus_tag="PLC71_09455"
     CDS             complement(1908..2597)
                     /locus_tag="PLC71_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM02904.1"
                     /translation="MKLFCKDFLHFTKEAFSRFFKILWPSFLSNTLWALATPIQTGIL
                     GRLSADAIAANSVSTTMFQYLKVITIGEASASAVLIGTTIGEGKGTTKVKEYSKTLQV
                     IYLIIGSVLGISLFFLRIPLLSLYDLTQNAYQMADQILIILSIVMVGMSYQMPTGIGI
                     IKGGGDVKYMMYLNLISTWAIVMPLSFLGAFVWKLPVPMVVLLLNSDQLFKCIPTYLY
                     VKKDQWIKVLV"
     gene            complement(2690..>3011)
                     /locus_tag="PLC71_09460"
     CDS             complement(2690..>3011)
                     /locus_tag="PLC71_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRM02905.1"
                     /translation="PPYYTPLGIDGKPAKFLKAKPKTKSNCDHCDLCVKVCPMGSINS
                     EDPSKIDGICIKCQACVKKCPKQAKYFDDPAFLSHVEMLKRNYQRAAKNEIFVSDGRE
                     NEIQ"
BASE COUNT          813 a          607 c          505 g         1086 t
ORIGIN      
        1 acttcagatc cgaaaactgg aatatttaat ttgtttgctt tctgaaggta tgtctgcatg
       61 ttgctgacta ctaaattgtc tgttaagttt gtgatgcagt ctactttctt cagtaaactg
      121 tctgctgcca ttggcatatc tgcggaagct gtgattccct gtgtgatgat cttgaatcca
      181 tatttatcag ctactttttc ataagcttcg attcctgctt tggagtttgc ttcgtttgtg
      241 ctgtagaaga ttccgacatt ctttgctttt ggcatcatct gacgaatcag ttttaactgt
      301 ttatctgcaa gtacaagatc ggaagttcct gtgatatttc caacattctt tccttcttta
      361 tctacaaatc ctgcagcttt tggtgatgta acagctgtat aaacaactgg gatatcactg
      421 tcttttgctg cattgtatgc actctgtgca cttggtgttg cgatcgcaca gatcatatct
      481 aatttctttg atgcaaagtt tgatgcgatc tggttatctg tggctgtatc tgcctgtgag
      541 tttttgtatg taatctttaa gttttctcct tctttgatac cttcagattc taaacctttt
      601 aaaaatcctt tgcggcagtt atcaagagaa ccatgttctg caaactggat gattcctaag
      661 ttatatgttt tagacgaact gtcatctttc ttgcttccac atccaaccat taatgacatt
      721 gccataactg ctgttaatcc tgctgctaat acttttttca ttgttctcat aatttcttct
      781 cctttttatt taagttgtac gtttcttcaa atagaaaaaa agaaaagccc caagcaaata
      841 tgcttgaggc gaattatccg cggtaccact caaattgaca ctaggtgtgt ccacttaaat
      901 catatactat catatatgcc cactctggta acgggtcggg tgcccggcga ttcctacttg
      961 ttactttcaa aatcaccctc gaaagtccat tcaccaggga aatcatactg caatctcacc
     1021 acctgcagct ctctttgaat cattctaatc ctgcttacta ctctttctca acggtttctt
     1081 ttctatttgt ttaaaatcat aatactttta tctttccttg tcaagcatta tttgtaaaaa
     1141 gaatgtcctt gataggtaaa caatctggta agtgcctcat caaaccatga aacatttttg
     1201 ggatttgcca ttgttttttc tacgaaatac aaagctcctt cggaatagtc gattccatct
     1261 tctaatgctt tttttacgct tcttttcgta ctctttgtga tacgaacaga ttcatatctt
     1321 ccatctgctg tcggtgaaaa ctggactttt ccataggctc tttgaaacac aacggattca
     1381 attgaatttg gaaattcttt acttcttaca cggtttaaaa ttacatttgc tacaagtatc
     1441 ttgctttttt catctttatc ggttgcttct gcttccacga tcctctcaag aatcgatcgt
     1501 tctctttggg tgactacatg ctttgttttt ggaatataga cgggtttgtc tttaatctgt
     1561 tgttctaaat tgactttcgt cataagctgt gcctgcataa ggtacgctct ttgttcttcc
     1621 aaaagtcttg tctgcaccac ttgttctact ttggcattgg tttgggctgg taatcttagg
     1681 aattctcctg cgaagacatt ttgtccaaag ctgcagatgc cgatggaaca taggatgact
     1741 gcgatttcta ttttcttctt caatgtcaca tactctcctt taagaaagag tatatgcagt
     1801 tatttttaga ttattcatag aagtttcatt gccttagtgt gttgcaattg attttgtttt
     1861 atcaattatc ttatcatttg aatctgcaaa tggcaaggta ttgatcatca aaccaaaacc
     1921 ttgatccact gatccttctt cacatacaaa tacgttggta tacacttaaa caactgatct
     1981 gaattcagca ataacacaac catcggtact ggcaatttcc atacaaatgc ccctaaaaat
     2041 gaaagcggca tcacgatcgc ccaggttgag attagattca gatacatcat atattttacg
     2101 tcgccaccgc ctttgataat accaattcca gttggcatct ggtatgacat accaaccatc
     2161 acgatggata aaatgatcag aatctggtct gccatttgat atgcattttg tgtcagatca
     2221 tagagagata acaatggaat ccttaggaaa aacaagctga ttccaagaac gcttcctatg
     2281 atcagataaa taacttgcag tgttttactg tattctttta cttttgtagt tcctttgcct
     2341 tccccgatcg ttgttccgat caatacagca gatgctgagg cttctccgat ggttataact
     2401 ttcaagtact gaaacatcgt cgtagaaaca gaattggctg caatcgcatc tgcggataag
     2461 cggcctaaga ttcctgtttg aattggtgtc gcaagtgccc ataacgtatt tgataaaaag
     2521 gatggccaca aaatcttgaa aaagcgtgaa aatgcttctt ttgtaaagtg gaggaaatct
     2581 ttacagaata atttcaattt atgatccgca aagaaaatag caaaaacacc attttgctga
     2641 taaattaatt atatcagaaa aatgatgttt tcatccactt gattaaaaat tattgtattt
     2701 cattttctct gccatcactc acaaaaattt catttttagc agctctttga tagtttctct
     2761 ttaacatttc aacatgtgat aaaaatgccg gatcatcaaa atattttgct tgttttggac
     2821 atttttttac acatgcctga catttgatac aaattccatc aattttactt ggatcttctg
     2881 aattgatcga tcccattgga cagactttca cacaaagatc acagtgatca caattacttt
     2941 ttgtctttgg ttttgccttt aagaatttcg ctggtttgcc atcaattcca agtggggtat
     3001 aatatggcgg g
//
LOCUS       DAOMUE010000253         2998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9391_length_2998_cov_3.585457, whole genome shotgun sequence.
ACCESSION   DAOMUE010000253 DAOMUE010000000
VERSION     DAOMUE010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9391_length_2998_cov_3.585457"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /gene="rpsH"
                     /locus_tag="PLC71_09465"
     CDS             complement(<1..327)
                     /gene="rpsH"
                     /locus_tag="PLC71_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRM02906.1"
                     /translation="MTMSDPIADMLTRIRNANTAKHDTVEIPASKMKTAIAEILLKEG
                     FIKAYDIKEEGSFSTIVITLKYGADKNEKIITGLKRISKPGLRVYAGAEELPKVLGGL
                     GIAIIST"
     gene            complement(352..537)
                     /locus_tag="PLC71_09470"
     CDS             complement(352..537)
                     /locus_tag="PLC71_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRM02907.1"
                     /translation="MAKKSMVAKQKRKAKFSTREYSRCRICGRPHAYLRKYGICRVCF
                     RELAYKGQIPGVKKASW"
     gene            complement(552..1091)
                     /gene="rplE"
                     /locus_tag="PLC71_09475"
     CDS             complement(552..1091)
                     /gene="rplE"
                     /locus_tag="PLC71_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRM02908.1"
                     /translation="MSRLHEQYSNQIKDAMMKKFEYSNIMQVPKLEKIVINMGVGEAK
                     ENKKVLESAVADLEKIAGQKAVVTRAKKSVANFKLREGMPIGCKVTLRGERMYEFADR
                     LINLALPRVRDFRGVNQNSFDGRGNYALGIKEQLIFPEIEYDKVDKVRGMDIIFVTTA
                     NTDEEARELLTLFGMPFKK"
     gene            complement(1114..1425)
                     /gene="rplX"
                     /locus_tag="PLC71_09480"
     CDS             complement(1114..1425)
                     /gene="rplX"
                     /locus_tag="PLC71_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRM02909.1"
                     /translation="MSAIKIKKGDLVKVIAGKDKDKEGKVIAVDHKKGTVTVEGANMI
                     TKHTKPSPANQQGGIVSQEGPMDVSNVMLVHNGKTTKIGYKVVDGKKVRVARATGEVI
                     D"
     gene            complement(1438..1806)
                     /gene="rplN"
                     /locus_tag="PLC71_09485"
     CDS             complement(1438..1806)
                     /gene="rplN"
                     /locus_tag="PLC71_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRM02910.1"
                     /translation="MIQQESRLRVADNTGAKEILCIRVLGGSTRRYANIGDVIVATVK
                     DATPGGVVKKGDVVRAVVVRTKKGARRKDGSYIKFDENAAVIIKDDMNPRGTRIFGPV
                     ARELRDKKFMKIVSLAPEVL"
     gene            complement(1837..2091)
                     /gene="rpsQ"
                     /locus_tag="PLC71_09490"
     CDS             complement(1837..2091)
                     /gene="rpsQ"
                     /locus_tag="PLC71_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRM02911.1"
                     /translation="MERNLRKTRVGKVVSDKMDKTIVVAIEDNVKHPLYKKIVKRTYK
                     LKAHDENNECNIGDRVKVMETRPLSKDKRWRLVEIIEKAK"
     gene            complement(2119..2322)
                     /gene="rpmC"
                     /locus_tag="PLC71_09495"
     CDS             complement(2119..2322)
                     /gene="rpmC"
                     /locus_tag="PLC71_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRM02912.1"
                     /translation="MKTKDFVKELNNKSVDELNNELVAAKKELFNLRFQNATNQLENT
                     SRIKEVRRNIARIQGAITAKANA"
     gene            complement(2312..2749)
                     /gene="rplP"
                     /locus_tag="PLC71_09500"
     CDS             complement(2312..2749)
                     /gene="rplP"
                     /locus_tag="PLC71_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRM02913.1"
                     /translation="MLMPKRVKRRKQFRGSMRGKALRGNKITYGEYGIVATEPAWITA
                     NQIEAARVAMTRYIKRGGKVWIKIFPDKPVTAQPAETRMGKGKGNLEYWVAVVKPGRV
                     MFEISGVSEEIAKEALRLAVHKLPIKCKIVSREALEGGDNSEN"
     gene            complement(2749..>2998)
                     /locus_tag="PLC71_09505"
     CDS             complement(2749..>2998)
                     /locus_tag="PLC71_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRM02914.1"
                     /translation="SCMQRTMRSGALGIKTSVSGRLGGADMARTEFYSEGTIPLQTLR
                     ANIDYGFAEADTTYGKVGVKTWIYHGEVLPSKKEGGNK"
BASE COUNT          728 a          675 c          455 g         1140 t
ORIGIN      
        1 tgtagagatg atcgcaatac caagtcctcc taatacttta ggaagctctt cagctcctgc
       61 gtatactcta agtcctggtt tagagattct cttaagtcct gtaatgattt tttcattttt
      121 atcagcacca tattttaatg tgattacgat tgtgctgaaa gaaccttctt ctttgatgtc
      181 atatgctttg ataaaacctt cttttaataa gatttctgca atcgctgtct tcatcttaga
      241 agcaggaatt tctactgtat catgtttcgc agtatttgca ttacggattc ttgtaagcat
      301 atctgcaata ggatcgctca ttgtcatttt cgtgttcctc cttccttaat cttaccagct
      361 tgccttcttg acacctggaa tctgtccttt atatgctaat tcacggaagc aaactctgca
      421 gataccatat tttcttaaat atgcatgtgg acgtccgcag attctgcaac ggctatattc
      481 tcttgttgag aattttgctt ttcttttctg ttttgcaacc attgacttct tagccatggg
      541 aaccctccta actatttctt aaacggcata ccaaataaag ttaataattc acgagcttct
      601 tcatcagtgt tcgctgttgt aacgaagatg atgtccattc ctcttacttt gtctacttta
      661 tcgtattcga tttctgggaa aattaactgt tctttaatac ctaatgcata gttacctctt
      721 ccatcgaaag agttctggtt aactcctctg aagtcacgta ctcgaggtaa tgctaagtta
      781 attaaacgat cagcgaattc atacattctt tcaccacgta atgtaacttt gcatccgata
      841 ggcatacctt ctcttaattt gaagttcgca acagatttct ttgctcttgt tacgactgct
      901 ttctgtccag cgatcttttc aagatcagct actgcagatt ctaaaacttt tttattttct
      961 tttgcttcac caacacccat gttgattacg atcttctcga gttttggtac ctgcataata
     1021 ttgctgtatt cgaatttctt catcatagca tctttgatct gattgctata ctgttcatgt
     1081 aatctactca cttgcgtaaa cctcctctct taattagtca atgacttcgc ctgttgcacg
     1141 tgcaacacga acttttttac cgtcaactac tttatatcca atttttgttg tttttccatt
     1201 gtgcactaac attacattgg aaacatccat tgggccttcc tggcttacga taccaccctg
     1261 ctggtttgca ggactaggtt tagtatgctt tgtgatcatg ttggcacctt caactgttac
     1321 agtacctttt ttatgatcaa cagcaattac ttttccttct ttgtctttat ctttaccagc
     1381 gataacttta actagatcgc cttttttaat tttaattgct gacacagtgt ttcctcctta
     1441 taatacttct ggtgctaatg aaacaatctt catgaatttc ttgtctctaa gctctcttgc
     1501 cactggtccg aagatacgag ttcctcttgg attcatatca tctttaataa ttacagcagc
     1561 attttcgtca aatttgatat aagaaccatc tttacgacgg gcacctttct ttgtacgaac
     1621 aacaactgct ctgactacgt cacctttttt tacaacgccg cctggtgttg catctttgac
     1681 tgtagcaaca ataacatcac cgatgttagc atatcttctt gtagatcctc ctaacacacg
     1741 aatgcaaagg atttcttttg ctcctgtgtt gtcggctact ctaagtcttg actcctgctg
     1801 aatcatgcct ttattctcct ttccaaccaa gcaaaattat tttgcttttt cgataatttc
     1861 aacaagtctc catctcttat cttttgaaag aggtcttgtc tccataacct ttactctatc
     1921 tccaatgtta cattcgttat tttcgtcatg agcttttaat ttgtaagttc ttttaacgat
     1981 ctttttatat aaaggatgtt ttacgttgtc ttcgattgca actacgattg ttttatccat
     2041 tttatcacta acgactttcc ctacacgggt ttttcttaaa tttctttcca caacaaattc
     2101 ctcctttcga aatgattact aagcgtttgc ttttgctgta attgcaccct gaatacgtgc
     2161 aatatttctt ctaacttctt taattctgct tgtattctca agttggtttg ttgcattctg
     2221 gaatcttaag ttgaataatt cctttttcgc agctactagc tcattattta attcgtctac
     2281 tgacttatta tttaattctt ttacaaaatc tttagttttc actgttgtca ccgccttcta
     2341 aagcttcacg agaaacaatc ttacacttaa ttggtaattt gtgtactgca agacgtaatg
     2401 cttcttttgc aatttcttcg cttacaccag aaatttcgaa cattacacgt cctggtttta
     2461 caactgctac ccaatattct aagttacctt tacctttacc cattcgagtt tcagctggct
     2521 gagctgttac tggtttgtca gggaaaattt tgatccaaac tttaccacca cgtttgatgt
     2581 aacgagtcat agcaacacgg gctgcttcga tctggtttgc tgtgatccat gctggctctg
     2641 tagctacaat accgtactca ccgtaagtga tcttattacc gcgtaatgct tttccgcgca
     2701 tgcttccacg gaactgttta cgacgtttta ctctttttgg cattaacatt atttatttcc
     2761 cccttccttt tttgatggaa gtacttcacc atggtaaatc catgttttta ctccgacttt
     2821 accatatgtt gtatctgctt ctgcaaatcc gtaatcaatg ttagcacgaa gtgtctgtag
     2881 aggaattgtt ccctcgctgt agaattctgt acgtgccata tctgctccac ctaaacgtcc
     2941 agaaacggat gttttgattc caagagctcc agatctcatt gtacgctgca tgcaagat
//
LOCUS       DAOMUE010000254         2996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9403_length_2996_cov_2.114247, whole genome shotgun sequence.
ACCESSION   DAOMUE010000254 DAOMUE010000000
VERSION     DAOMUE010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9403_length_2996_cov_2.114247"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..923)
                     /locus_tag="PLC71_09510"
     CDS             complement(159..923)
                     /locus_tag="PLC71_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439860.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HRM02915.1"
                     /translation="MNLYEIYFSPTGGTKKVADIMIKAMKKDAQEIDMIKDPDKILQT
                     EFSEDDICLIAVPSYGGRIPSVTVDKFQKLQAKGTKAILVAVFGNRAIDDTLVEMQDI
                     LENAGFVCVVGVEAVAEHSLMHQFGTGRPDQQDEKELIRFSEQIMQKIETKMECMKVE
                     LPGNHNYREYNGVPLKPVANGKCTSCGICAKECPAGAISKDDPKVTDKEKCISCMHCV
                     NICPRHARKLNPLLLSVASKKLKKVCFNRKENELFL"
     gene            complement(1072..1848)
                     /locus_tag="PLC71_09515"
     CDS             complement(1072..1848)
                     /locus_tag="PLC71_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HRM02916.1"
                     /translation="MARPTRCRRICMEPAYDSFFPDGFLTGEEVIMTLDEYETIRVVD
                     LAEYTQHECAKQMNISRTTTAEIYKSARYKMADSVVNGKKLLITGGNYQFCDGTTCPF
                     CKKHCNRAANSVRQSKILKKEGKKMRIAVTYEDGKIYQHFGHTSQFKLYDIEDGKITS
                     TQIVETNGQGHGALSGFLSQGEVDVLICGGIGGGAQIALAEAEIKLYGGVSGDADEAV
                     NAYLEGKLDFNPNVQCSHHAHEHSCGGHQCGEDKHGCSGN"
     gene            complement(1928..2803)
                     /locus_tag="PLC71_09520"
     CDS             complement(1928..2803)
                     /locus_tag="PLC71_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HRM02917.1"
                     /translation="MIANSRCIACNLSKKEEKIRKHSDEKKKKEFIQKVTGILYKYGD
                     IESTPMLDKRIQDIYNEYYEEETDYVALKHKYNQYMLEREDEIKEHLKEAKNIKDYIK
                     YVCVGNYIDFGVTGNIDDQMLDDLLKKVDSLNIPEQEIKYLEEELKQAKTLLYITDNC
                     GEIVLDKIFIEELKKRYGNLEITVMVRGGLAINDATIEDAQEIGLTKIVPVISNGAAI
                     AGTVKEQLSKEARKQLDSADVIIAKGMGNFESMYQEGINPYYLFLCKCDLFTRRFGVK
                     LFDPIFCKEERLDIK"
BASE COUNT          834 a          614 c          385 g         1163 t
ORIGIN      
        1 agcgtgcttg tgcggatgat ttgattatgc aatataaaga aggtgacaag ggggaaaaga
       61 ggattaaata gacagagaat aaaatttgaa aatcggagaa ttgaggggat tacggtttgt
      121 ggatcgtaat tccctcaatt acatttgaag gtgattgttt ataaaaacaa ttcgttttct
      181 tttcgattaa aacagacttt ttttaacttt tttgaggcaa cagatagtag cagtggattt
      241 agtttacgtg catgtctagg acagatattg acacaatgca tacaggaaat acatttctct
      301 ttatctgtta cttttggatc atcttttgaa atcgctcctg ctggacattc ttttgcacaa
      361 attccacagg aagtacattt gccatttgca acaggtttta aaggtacacc attatattct
      421 ctataattgt gatttcctgg tagttctacc ttcatacact ccatttttgt ttctattttt
      481 tgcatgatct gttctgaaaa tctaatcaat tctttctcat cctgttgatc tggccgtcca
      541 gttccaaact gatgcatcag agaatgttct gctactgcct caactcctac tacacaaaca
      601 aatcctgcat tttctaaaat atcttgcatt tcaactaatg tatcatcaat cgctctgttt
      661 ccgaatacag caaccaatat tgcttttgtc cctttcgctt gtaatttttg aaatttatca
      721 actgtcacag atggaattct acctccataa gaaggaactg cgatcagaca gatatcatct
      781 tccgaaaatt ctgtctgtag tattttatct ggatctttga tcatatcaat ctcttgtgca
      841 tcttttttca ttgctttgat cataatgtct gctacttttt ttgttccacc tgtaggacta
      901 aaataaattt catataaatt catatatttt tctcctattg ttctcttttt tatatacaaa
      961 gttctccatc tgcttaaatc ttcagatgga gaacttgctt gatgtcttat cctattgtca
     1021 ttatactctt tctacatatt tttgtaaatt attattcttt tgcagtactt tctaatttcc
     1081 tgaacatcca tgcttatctt ctccacattg atgtcctcca caagaatgtt catgtgcatg
     1141 atgagaacat tgtacattag gattgaaatc tagttttccc tctaaatatg cattcacagc
     1201 ttcatcggca tctcctgata cgccaccata taatttaatt tcagcttctg ctaatgcaat
     1261 ttgtgctcca ccaccgattc cgccacagat caatacatcc acttcacctt gtgataagaa
     1321 acctgataag gctccatgtc cttgtccatt cgtttcaacg atctgtgtag atgtaatctt
     1381 accgtcttca atatcataaa gtttaaactg acttgtatgt ccaaaatgct gataaatctt
     1441 tccatcttcg tatgttactg caattctcat tttcttacct tcctttttta aaatctttga
     1501 ttgtctgacg gaatttgctg ctctattgca atgtttttta caaaaaggac atgtcgtgcc
     1561 atcgcaaaac tgataatttc ctcctgtgat cagaagcttc tttccattca caacgctgtc
     1621 tgccatcttg tatcttgcgc ttttataaat ctctgctgtg gttgtcctgg aaatattcat
     1681 ctgttttgca cattcatgct gtgtatattc tgcaagatcg accacacgta tcgtttcgta
     1741 ttcatctaat gtcatgataa cttcttcccc tgtcaaaaat ccatctggaa aaaaactatc
     1801 ataagctggt tccatacata ttcttctaca tcttgttggt cttgccatat tatcttctcc
     1861 ttgttgtatt ttatttccgt catatgtcga taactgtatt ataacattca aatatttata
     1921 tctcaagtta ctttatatct aatcgttctt ctttgcagaa tatcggatca aacaacttga
     1981 ccccaaatct ccgtgtgaac aaatcacatt tgcataggaa taaataatat gggttgattc
     2041 cttcctgata catagattca aaattgccca tgcctttggc tatgatcaca tctgcactgt
     2101 ctaactgttt tctcgcttct ttgcttaact gttctttaac cgttccagca attgcagctc
     2161 cattactgat aactggaaca atctttgtaa gacctatttc ttgtgcatct tctatcgttg
     2221 catcattaat tgccagacca cctcttacca taactgtgat ttcaagattt ccataacgtt
     2281 tttttaattc ctcaataaaa atcttatcaa gaacgatttc accacaatta tctgtgatat
     2341 acaaaagcgt ttttgcttgt tttaattctt cttccagata tttaatttct tgttcaggta
     2401 tatttagact atcaactttc ttcaataaat catctaacat ctgatcatct atatttccag
     2461 tgacaccaaa atctatataa tttccaacac atacatattt aatataatct tttatattct
     2521 ttgcttcctt caaatgctct ttgatctcgt cttctctttc caacatatat tgattatatt
     2581 tatgttttaa cgctacataa tccgtttcct cttcataata ttcattataa atgtcctgaa
     2641 ttcttttatc caacattggt gttgattcta tatccccata tttatataaa atccctgtaa
     2701 ctttctgaat aaattccttt ttcttttttt catctgaatg ttttcgaatt ttttcttctt
     2761 ttttcgaaag attacatgca atacatcttg aattcgcaat catgattttt ctccttatgt
     2821 catcaaagtc ttattctaat cctgctttta ttatatcatg agggtatttt ctatgaaata
     2881 aaaaaagcat ccttatctct aagaatgctc tgaatttcat tattaaatct atatataaag
     2941 gatatacctt caaaacaaca tacaagaatc tttctacatc tcttacactt cagcct
//
LOCUS       DAOMUE010000255         2989 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9432_length_2989_cov_2.231766, whole genome shotgun sequence.
ACCESSION   DAOMUE010000255 DAOMUE010000000
VERSION     DAOMUE010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9432_length_2989_cov_2.231766"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..862)
                     /locus_tag="PLC71_09525"
     CDS             complement(155..862)
                     /locus_tag="PLC71_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRM02918.1"
                     /translation="MYPYGYGYYFDPTYILIIIGVIITMIASSKMNSTFRRYSRVRCH
                     CGLTGSQAAERILNQAGIYDVSVERVSGNLTDHYDPKNKVLRLSDATYGSDSIAAIGV
                     AAHECGHAVQHQQGYAPLKIRGAMVPAVNLGSNLSWLFILGGVILGMNQTLLNIGILL
                     FSLAVIFQLVTLPVEFNASSRALRILGSTGIMYEDEVGQTRKVLSAAALTYVAGAASA
                     LLSLLRLIILFGGRDND"
     gene            complement(982..1965)
                     /locus_tag="PLC71_09530"
     CDS             complement(982..1965)
                     /locus_tag="PLC71_09530"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HRM02919.1"
                     /translation="MIRIGIMGYGNLGRGIECAIKQNDDLELVAVFTRRDPATVSILT
                     EGAAVCNVKDVEDWKDKIDVLMLCGGSATDLPVQTPEYAKLFNVVDSFDTHARIPEHF
                     ANVDKAAKEYGHIGIISVGWDPGMFSLNRMYANAILPEGKDYTFWGKGVSQGHSDAIR
                     RVEGVKDGKQYTIPVEAALEAVRNGEDPELTTRQKHTRECFVVLEEGADAKKVEEEIK
                     TMPNYFSDYDTTVHFISQEELDRDHSKIPHGGFVLRSGCTGWEKENKHIIEYSLKLDS
                     NPEFTSSVLVAYARAAYKLSKEGQSGCKTVFDIAPAYLSAKSGDELRASLL"
     gene            2124..2738
                     /locus_tag="PLC71_09535"
     CDS             2124..2738
                     /locus_tag="PLC71_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02920.1"
                     /translation="MSFTGIFKAKNGIVAVVDSKASRVENGQSYEDVHRNPEKLFVFQ
                     NGIATTFGANQILVQNPFEFSSKVIAIEDLVYEYLHLKNILDASFFQSLLVKMTSNPA
                     NTEPVNFLVGRKIRQEEYILEHHQVRYCAYAQRLATPMEHFFTGGDEKYRKHFDNLPY
                     LSQITSVDVLQKTVAKELDGLIQYYDQQSEYNPVGKPVKSYVLR"
     gene            complement(2746..>2989)
                     /locus_tag="PLC71_09540"
     CDS             complement(2746..>2989)
                     /locus_tag="PLC71_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HRM02921.1"
                     /translation="TNARCPVCHKKMEMVGEGEGQKFVCVCGYKEKLSAFKQRKEKQG
                     SGMSKKEVNRYLKKQQKEAQAPINNAFAEAFSKIKL"
BASE COUNT          848 a          662 c          462 g         1017 t
ORIGIN      
        1 catatagatc cctggttttc ttcctttttt tactgcgtaa aattttcctg ccatatattt
       61 cctcttattt ctttaatagt cttaatcctt atattataat tttctttgaa aagaaaaaat
      121 cctgcattgc aggatttttc ctaaccttat gattctagtc gttatctctt ccaccaaata
      181 aaatgatcag acgaagcaag ctcagaagtg ctgatgctgc acctgctaca tatgtcagtg
      241 cagctgctga taaaaccttc cttgtctgcc ccacttcatc ttcgtacatg atccctgtac
      301 ttccaagaat tcgtaacgct cttgaagatg cattaaattc caccggaagt gttactaatt
      361 gaaagatcac cgctaatgaa aataataaaa ttccaatatt caataatgtc tgattcattc
      421 caaggatcac tccacctaga ataaacaacc aggaaagatt ggatccaaga ttcacagctg
      481 gaaccatagc cccacgaatc ttcaatggtg catatccttg ctgatgctgt acagcgtgcc
      541 cacactcatg ggctgcaaca ccgatcgctg cgatcgaatc agatccataa gttgcatctg
      601 ataatcttaa tactttattc tttggatcat aatgatctgt aagattcccg ctgacacgtt
      661 ctaccgaaac atcgtaaatt cctgcctggt ttaaaatacg ttcagcagcc tgagaacccg
      721 tcagtccgca atgacaacgg actcttgaat atcttcgaaa cgttgaattc atcttggaag
      781 atgcgatcat cgtgatgatc actccgatga tgatcaaaat atacgttggg tcaaaataat
      841 aaccataacc gtatggatac ataaatatct cccttctttc tctgtaacat gtagatgatt
      901 atatcgttcc tcctacatga aatcaaggca gtttagaaat attttataat cattttattg
      961 aatgattaat tcttcatatt tttataacaa agaagctctt aattcgtctc cggatttcgc
     1021 actcaaatat gctggcgcaa tatcaaatac agtcttacat ccactctgtc cttctttgga
     1081 aagtttatat gcagctcttg catatgcaac taaaacactt gatgtaaatt ctgggttaga
     1141 atctaatttt aagctgtact caatgatatg tttgttttct ttttcccatc ctgtacatcc
     1201 acttcttaat acaaatccac catgagggat cttgctatgg tcacgatcta attcttcctg
     1261 gctgatgaaa tgtacagttg tatcatagtc tgagaagtag ttcggcattg tcttgatctc
     1321 ttcttcaact ttcttcgcat ctgcaccttc ttcaagtact acaaagcatt ctcttgtatg
     1381 cttctgtctt gttgtaagtt ctggatcttc cccattacga actgcttcta aagctgcttc
     1441 tacaggaatt gtgtactgtt taccatcttt gacaccttcc acacgacgga tcgcatcaga
     1501 gtgtccctgg ctgactcctt ttccccagaa tgtataatct tttccttctg gaaggattgc
     1561 atttgcatac attctattta aagagaacat tcctggatcc caacctacag aaatgattcc
     1621 aatatgtcca tattctttcg ctgctttatc tacatttgca aaatgttctg gaattcttgc
     1681 gtgtgtatca aagctgtcta ccacgttaaa taatttggca tattctggtg tctgaacagg
     1741 aaggtctgtt gcacttcctc cacataacat taaaacatcg atcttatctt tccaatcttc
     1801 tacatctttg acattacata cagctgcacc ttctgttaaa atgctgactg tcgcgggatc
     1861 tcttcttgta aatacagcta caagctccaa atcgtcattc tgtttgatcg cgcattcaat
     1921 tccgcgtcca aggttaccat aacccataat accaattcta atcattgatt ttctcctttt
     1981 tgactttatt aaacatagta tatcatttct gtgttttgcg ttcaatgtat tctttttatg
     2041 atttttttgc tataatgatc ttaaaaattc ccgatgattc ctgatccttc gggaatattc
     2101 taacagaaat gaggaatttt atcatgtcat ttaccggcat tttcaaagca aaaaacggca
     2161 ttgtcgctgt cgttgattca aaggcatcca gagtagaaaa tgggcaatct tatgaagacg
     2221 ttcacaggaa tccagaaaag ctctttgtat tccaaaacgg aattgcaaca acttttggtg
     2281 ctaaccagat tttagtccag aatccttttg aattttcctc aaaagtcatt gccatcgaag
     2341 atctggttta tgaatatcta catttaaaga atatcttaga tgcttctttc ttccaatctc
     2401 tgcttgtaaa aatgacatcc aacccagcca atacagaacc tgtaaacttt ctggttggac
     2461 gcaaaatccg tcaagaagaa tacatcttag aacatcatca agtacgttat tgcgcctacg
     2521 cacaacggct cgcaacccca atggaacatt tctttactgg cggagatgaa aaatatcgga
     2581 aacactttga taaccttcca tatctttcac agatcacctc tgttgatgta ttgcagaaaa
     2641 ctgttgcaaa agaattggat ggattgattc aatattatga ccagcaatcc gaatataatc
     2701 ctgtaggaaa gcctgtgaaa tcttatgttt taagataatg ctactttata gttttatctt
     2761 cgaaaaagcc tccgcaaatg cattattgat cggcgcctgt gcttcttttt gctgtttttt
     2821 caaatatcgg ttcacttctt ttttactcat tccagaacct tgtttttctt ttctttgttt
     2881 aaatgcagac agtttttctt tatacccgca aacacataca aacttctgtc cttcaccttc
     2941 tccgaccatt tccatcttct tatggcatac gggacatctg gcatttgtg
//
LOCUS       DAOMUE010000256         2978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9466_length_2978_cov_1.867944, whole genome shotgun sequence.
ACCESSION   DAOMUE010000256 DAOMUE010000000
VERSION     DAOMUE010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9466_length_2978_cov_1.867944"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="PLC71_09545"
     CDS             <1..1398
                     /locus_tag="PLC71_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM02922.1"
                     /translation="MLQEDSLLKIMRTNCCMFDPVKPCTHCFQCIGLIDQKSPKELNA
                     FESAKLFEYKLYEMNIDQFEKRINESFHKNGGFEDIIFSMNYDVDQMLQVNAEAYNKE
                     RDISISVERLGRGMKSIYMLSLLEAYVMDENSLSSIILVEEPEMFLHPQLQKTASEIL
                     YRLAQKNQVIFTTHSPHLLANFSSRQLCQIILDEDSYSVAKKKTNISSVLDDLGYNAS
                     DLMNVDFVFIVEGKQDKYRLPLLLNHYYSEIYDEDGRLNRVAILTTNSCTNIKTYANL
                     KYINQLYMKDRFLMIRDSDGKDRDMLGRQLCKYYDERNLVDVDHLPKVTRKNVLILKY
                     YSFENYFLNPEIMSKLGVIESEDAFYEILYDKWREYLHRIKSGVHLIQMMGRDFTSPQ
                     DMKEHMEEIKTYMRGHNLFDIFYGRYKKEEKDLLKKYIEIAPRDEFSDILDAADSFIY
                     FQSKTKQKDIQNETN"
     gene            1420..>2978
                     /locus_tag="PLC71_09550"
     CDS             1420..>2978
                     /locus_tag="PLC71_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HRM02923.1"
                     /translation="MTKKHTILTAINSKYIHSNLAVYCLRAYAKPYIDEVEIAEYTIN
                     QQVDDILMSLYQKHPDILCFSCYIWNIEYIERVIREIHKLLPDVPIWLGGPEVSYDSR
                     EVLRRLPQVTGVMKGEGEVTFLEVLDHYHGKRDDLSKIPGLTYRNEDEIVETPWRPVM
                     DLSKVPFVYEHIEDFEHKIIYYESSRGCPFSCSYCLSSIDKCLRFRNLSLVKKELQFF
                     LDHKVPQVKFVDRTFNCRHDHAMEIWRYIKEHDNGITNFHFEVAADVLKDDEIELISD
                     MRPGLIQLEIGVQSTNIDTITEIHRKMDFKKVADIVTRINAGHNIHQHLDLIAGLPYE
                     DLESFKQSFQDVYEVHPEQLQLGFLKVLKGSYMEKQKENYGLVYKDTPPYEVLYTKWL
                     PYEDVLVLKKVEEMVEVYYNSSQFSNTLRLLEKEFDTAFALYDTLARFYEEKGYDKVS
                     HSRIARFEILYEFIQTITAEENLVLYKELLTYDLYLRENVKKRPDFAGDYTLQKDELR
                     YINEEILLKDER"
BASE COUNT         1018 a          435 c          612 g          913 t
ORIGIN      
        1 atgctacagg aagacagttt gcttaagatc atgcgtacaa actgctgtat gtttgatcct
       61 gtgaaaccat gtactcattg ttttcagtgt atcggtctga tcgatcagaa gagtccaaaa
      121 gagctgaatg catttgagtc tgcaaagtta tttgaatata aattgtatga gatgaacatt
      181 gatcagtttg agaaacgtat taatgagagc ttccataaga acggcggatt tgaggatatc
      241 attttctcta tgaattatga tgttgatcag atgcttcaag ttaatgcaga ggcttataac
      301 aaagaacgtg atatttcgat ttctgtggaa cgattgggtc gtggaatgaa aagtatttat
      361 atgctttctc ttctggaagc ttatgtaatg gatgagaata gtctttcatc gatcattctg
      421 gtagaggaac cagagatgtt tttgcatcca caactgcaaa agacagccag tgagatcctt
      481 tatcgtctgg cacagaagaa tcaggtgatc tttacaacac attctccaca tttattagcc
      541 aattttagca gtagacaatt atgtcagatc atattggatg aagattcata ttctgttgca
      601 aagaagaaaa cgaacattag ttcggtatta gacgatcttg gatataatgc ttccgatctt
      661 atgaatgttg attttgtttt tatcgtagaa ggaaaacaag ataaataccg tttgccattg
      721 ttgttaaatc attattattc tgaaatttat gatgaagacg gcagattaaa tcgtgtagcg
      781 atcctgacaa caaacagctg taccaatatc aagacgtatg ccaacttaaa atatataaat
      841 cagctttata tgaaggatcg ttttttaatg atccgtgata gtgatggtaa ggatcgagat
      901 atgcttggaa gacaactttg taaatattat gatgagagaa atcttgtgga tgttgatcat
      961 ttaccaaaag tgacaagaaa aaatgtattg atcttaaaat attattcttt tgaaaattat
     1021 tttttaaatc cagagatcat gtcaaaactt ggagttatag aatctgaaga tgctttttat
     1081 gagattttat atgataaatg gagagaatat ctgcatagga tcaaaagtgg agttcatttg
     1141 atacagatga tgggacgtga ttttacatca ccgcaagata tgaaagaaca tatggaagag
     1201 atcaaaacct atatgcgggg tcataatctg tttgatattt tttatggaag atataaaaaa
     1261 gaagagaaag atttattaaa gaaatatatt gagatcgcac caagagatga atttagtgat
     1321 attcttgatg cggcggattc gtttatctat tttcagagta agacaaaaca aaaggatatt
     1381 cagaatgaaa caaattaaag aaagtgcgag ataaataata tgacaaagaa acacacaata
     1441 ttaacagcga tcaattcgaa atacattcac tcgaaccttg cagtttactg ccttcgtgcc
     1501 tacgcaaagc catatatcga tgaagtcgag attgcagaat atacgatcaa tcagcaggtg
     1561 gatgatatcc tgatgagtct gtatcagaaa catccagata tcctttgttt ttcctgttat
     1621 atctggaata ttgaatacat agaacgtgtg atcagggaaa tccataaact actgccggat
     1681 gttccaatct ggcttggagg accagaagtt tcctatgatt ccagagaagt tttaagacga
     1741 cttccacagg taacgggtgt gatgaaaggg gaaggagaag ttacattcct tgaagtgctt
     1801 gatcattatc atggaaagag agatgatctt agcaagattc ctggactaac ttatagaaat
     1861 gaagatgaga tcgtagaaac accatggcgt ccagtgatgg acttaagtaa agttccattt
     1921 gtgtatgaac atattgaaga ttttgaacat aagatcatct actatgaatc aagcaggggc
     1981 tgtccatttt catgtagtta ttgtttatct tcgatcgata aatgcctgcg cttccgaaat
     2041 ctttctcttg tcaaaaagga attacagttt ttcttagatc ataaagtgcc acaggtcaaa
     2101 tttgtcgatc gtacctttaa ctgtcgacat gatcatgcaa tggaaatctg gcgttatata
     2161 aaggaacatg ataacggcat taccaatttc cattttgaag tggcagcaga tgtcttaaag
     2221 gatgatgaaa ttgaattgat ctctgatatg cgtccgggac tgattcagtt agagattggc
     2281 gttcagtcaa cgaacatcga tacgatcact gagattcaca ggaaaatgga ttttaagaaa
     2341 gttgcagata tcgtaacaag gatcaatgct ggacataata tacatcaaca tttggatctg
     2401 atcgcaggac tgccctatga agatcttgaa agttttaaac agtcatttca ggatgtctat
     2461 gaagtacatc cagaacaatt acagttaggc tttttaaaag tattaaaagg ttcttatatg
     2521 gaaaaacaaa aagagaacta tgggcttgtt tataaagata caccgcctta tgaagtcctt
     2581 tatacaaaat ggctgcctta tgaagatgta ctggttttaa agaaagtgga agaaatggtg
     2641 gaagtttatt ataatagcag ccagttttcg aacaccttgc ggttgttgga aaaagaattt
     2701 gacactgcat ttgctttata tgatacactt gccagatttt atgaggagaa aggttatgac
     2761 aaggttagcc acagccggat tgcccgattt gaaatcttat atgaatttat acaaaccatt
     2821 acagcagaag agaatcttgt attatataaa gagttgttaa cgtatgactt atatcttcgt
     2881 gaaaatgtaa agaaacgtcc ggattttgca ggagattata cattacaaaa ggacgagctt
     2941 cgttatatca acgaagagat tttattaaaa gatgaacg
//
LOCUS       DAOMUE010000257         2959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9548_length_2959_cov_2.433884, whole genome shotgun sequence.
ACCESSION   DAOMUE010000257 DAOMUE010000000
VERSION     DAOMUE010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9548_length_2959_cov_2.433884"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLC71_09555"
     CDS             <1..222
                     /locus_tag="PLC71_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM02924.1"
                     /translation="IMMPVMDGLTATKEIRSIKRDDAAQIPIIAMTANAFEEDAKKCF
                     AAGMNAHLAKPFQIDKVEKTLVQCIHKSI"
     gene            316..1269
                     /locus_tag="PLC71_09560"
     CDS             316..1269
                     /locus_tag="PLC71_09560"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRM02925.1"
                     /translation="MSKGINSRKVAVIGTGFVGSSSAFALMESGLFTEMVLIDADKNR
                     AEGEALDIAHGLPFARPMKITAGDYDDIVDAAIIVVTAGAGQKPGETRLDLVKKNVAI
                     FQSIIPEIAQRKCEGILLIVANPVDILTQVAVKLSGFPENRVFGSGTTLDSARLKYLL
                     GEHLQVDARSVHAWIIGEHGDSEIVAWSSANVSGVPISEFCEMRGYTEHDEHMEEIAQ
                     GVKNSAYKIIEKKKATYYGVAMAVRRICEAIIRDEKSVLPVSSIQHDTYGIEGVTLSM
                     PAIVGKNGIEKQLAIKLNEKEQEALKKSAEALKEVLENLDI"
     gene            1298..1459
                     /locus_tag="PLC71_09565"
     CDS             1298..1459
                     /locus_tag="PLC71_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02926.1"
                     /translation="MWFMIRKLQKTDINRVADIWLDTNLKAHDFIPAKYWKNNFQLVK
                     EYVMIWSQK"
     gene            1481..2851
                     /locus_tag="PLC71_09570"
     CDS             1481..2851
                     /locus_tag="PLC71_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM02927.1"
                     /translation="MEGNLTKGPILKTLTKLAVPIMASSFLGTLYNITDMAWIGLLGS
                     KAVAGVGVGGMFTWFSQGLASMSRMGGQVQVAQCIGRGDKEEAHKYAQTAVQLATLMG
                     LLFAAVMLLFLHPLVGFFQLQDPEALQAALSYTKIACGLIVFSFLTLTLTGIYTAQGD
                     SKTPFIANFIGLVTNMILDPLLILGLGPVNKFGVNGAAIATVSAQAIVMTILILGIIR
                     QKKQNVLKGIKLFTKIPFKYLSGICKIGIPTAIQGMVYCGISMVLTRMVSGFGAEAVA
                     VQRVGGQIESVSWNTADGFGTALNAFIGQNYGAGKMDRVRKGYKASLCSVGIWGIFIT
                     IMFVFQPKAIAEIFFHEPKAIMIAIGYLTIIGVSEAFMAVELMTVGALSGLGKTHLCS
                     VISILFTSARIPLAIILGGALLGLEGIWWAFSVTSIVKGIIFVCTFCWITRGKGTAKM
                     MKLNKE"
BASE COUNT         1010 a          445 c          694 g          810 t
ORIGIN      
        1 attatgatgc cggttatgga tggattgaca gcgaccaagg agattcgatc catcaaacga
       61 gacgatgcag cacagatacc gatcatcgct atgacagcaa atgcatttga agaagatgca
      121 aagaaatgtt ttgcagcagg gatgaatgca cacttagcga aaccatttca gattgataag
      181 gttgaaaaaa cattagtaca atgtattcat aagagtatat aataaacaat atagtaaaaa
      241 tctttttgca tgaattgata taattaaatt gggtgataat attgttattc taaaaatgta
      301 aaaggagacc caataatgag taaaggaata aacagcagaa aagtagcagt gatcggaaca
      361 ggatttgtag gttcatcttc tgcatttgca ttaatggaaa gtggattatt tactgaaatg
      421 gtgttgatcg atgctgataa gaatcgtgca gaaggagagg cattagatat tgcccatgga
      481 ctcccatttg ccagaccaat gaagatcaca gcaggcgatt atgatgatat cgtggatgca
      541 gcgatcattg ttgtgacagc gggtgcagga cagaaaccag gagagacaag acttgatctt
      601 gtcaagaaaa atgtagcaat ctttcaatcg atcattccag agatcgcaca gagaaaatgt
      661 gaagggattc ttttgatcgt agccaatcca gtagatattt tgacacaagt tgcagtaaaa
      721 ctatcaggat ttcctgaaaa cagagtcttt ggttctggta caaccctaga ttctgccaga
      781 ttaaaatatc tgcttggaga acatttacag gtagatgcaa gatctgttca tgcatggatc
      841 atcggagaac atggagattc tgaaatcgta gcatggtcaa gtgccaatgt atcaggcgta
      901 ccaatttcag aattttgcga gatgagaggt tacacagaac acgacgagca tatggaagag
      961 attgcacagg gagtcaaaaa cagtgcatat aagatcattg aaaagaaaaa agcaacatat
     1021 tatggagttg caatggcagt cagaagaatc tgcgaagcaa tcatccgaga tgaaaaatct
     1081 gtattaccag tatcttctat ccaacatgat acctatggaa ttgaaggtgt tacattaagt
     1141 atgccagcga tcgtaggaaa aaatggtatt gagaaacaac tggcgatcaa gctgaatgaa
     1201 aaagaacagg aagcattgaa gaaatcagca gaagccttga aagaagtgtt agaaaatctg
     1261 gatatttaaa aatttatttt tggccaggag gattcgtatg tggtttatga tcaggaaatt
     1321 acagaaaaca gatataaaca gagttgcaga tatatggtta gatacaaatt taaaagcaca
     1381 tgattttatt cctgccaagt attggaagaa caattttcaa ttagtaaaag aatatgtaat
     1441 gatatggtcg caaaaataaa ttcacaggag agaaaagtaa ttggaaggaa atttaacaaa
     1501 aggaccaatt ttaaaaacac taacgaaact tgcggtgccg atcatggcat cgtcattttt
     1561 aggaacatta tacaacatta cagacatggc atggatcgga cttcttggat caaaagcagt
     1621 tgctggagtt ggtgtcggag gcatgtttac gtggttttca caagggttag cttcgatgtc
     1681 gagaatggga ggacaggtac aggtagcaca atgcattgga cgaggagaca aagaagaagc
     1741 ccataagtat gcacagacag cagttcagct ggcaacgctt atgggacttt tatttgcagc
     1801 agtgatgtta ctgtttttgc atccgctggt aggattcttt cagctacaag atccagaggc
     1861 attgcaggca gcattatctt acacaaaaat agcctgtgga ttgatcgtgt tttcattttt
     1921 gacactcact ttaactggaa tctatacagc acaaggagat tcaaagacgc catttatagc
     1981 caattttata ggacttgtaa cgaatatgat cttggatcca ttactgatcc tggggcttgg
     2041 accagtaaac aagtttgggg taaatggagc tgcgattgca acagtaagtg cacaggcaat
     2101 tgttatgact attttgatcc ttggaattat ccgacagaaa aaacaaaatg tattaaaagg
     2161 aatcaagtta tttacaaaaa ttccatttaa atatctaagt ggaatctgca agattggtat
     2221 tccgacagcg attcagggaa tggtttactg tggaatttcc atggtattaa ctcgtatggt
     2281 ttcagggttt ggagcagaag cagttgcagt ccagagagtc ggagggcaga ttgaatctgt
     2341 ttcatggaat acagccgatg gatttgggac agctctaaat gcatttatcg ggcaaaatta
     2401 tggagcagga aagatggatc gtgtcagaaa aggatacaaa gcctctttgt gcagcgttgg
     2461 aatatgggga atctttatta cgatcatgtt tgtttttcaa ccaaaggcaa tcgcagagat
     2521 tttcttccat gaaccaaagg caattatgat cgcgatcgga tatttgacga tcattggggt
     2581 gagtgaagcg tttatggctg tagaactgat gacagtaggg gcattatcag gattaggaaa
     2641 aacacattta tgcagtgtga tcagcatttt atttaccagt gcaagaattc cgcttgcaat
     2701 cattttgggt ggagcattgc ttggactgga aggtatctgg tgggcatttt ctgtgacatc
     2761 aattgtaaaa ggaattatct ttgtatgtac attttgctgg atcacgagag gcaaaggtac
     2821 ggcaaaaatg atgaaactca ataaggaata atagaattat tgccgataat atagtttgat
     2881 catgtttaag ggatgtctaa aatatatatg tagactggaa aggatgtagc gatgaaaata
     2941 tatatagact tcgatgacg
//
LOCUS       DAOMUE010000258         2951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9579_length_2951_cov_1.864986, whole genome shotgun sequence.
ACCESSION   DAOMUE010000258 DAOMUE010000000
VERSION     DAOMUE010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9579_length_2951_cov_1.864986"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PLC71_09575"
     CDS             <1..1142
                     /locus_tag="PLC71_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM02928.1"
                     /translation="GPLENDYQYGRKVDLNTRQENKYSIVTKIVYGSNSFLMTGDAQK
                     ETIEKIIAKGYDLTAQVLKEPHHGFQDVTEEELANGYQYSDHKTVIDASQASIAIISN
                     GYMNTGGVPYTKVLRDLSKLDVYETGDRGTIIVTSDGSQLSIQTEKGDNQPSVKGKES
                     DDETSSPVMKSMNITANTTKPLTATSVDAANTYDRYEKKNITVRFSGTAQGFTKLTSI
                     EYKFVPKGVNNKTIAYKTGSSYTVKNGNCGRFYVRYNTPLGSTEIKLPGFTVDTKAPT
                     SVKIKANKSGIKTLSTSAKNTYSKRIKKSVKFTFSANYGTSGKSMTQYKFVPRGKKSS
                     KYKWKTGNSVTYKTRNKKVRLYVRYIDKSGNITTKKTNGFYVTKK"
     gene            1167..2930
                     /locus_tag="PLC71_09580"
     CDS             1167..2930
                     /locus_tag="PLC71_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM02929.1"
                     /translation="MKYFTKKIIAVLLTVMLTGITVLHVSGDQTIKVNAAQTFKVHFI
                     DVGAADGALLQYGEGENAKYALIDSGAYSYETTDHDTIDVSDRVHQYLLDHGVKHLEF
                     VVLTHPHGDHIGGMKKILEDKNITIDTIYGNPLEFEYLESSEDKEKQTEETARWTAFD
                     TQTYQTFKKKLEKRNSYQDASLHIQYVVPQAGTSIKLGEAVMTFYGPLDNTYRYGRAQ
                     DAPNLNTRQVNKYSIVTRIVYGSNSFLMTGDAQQETIKKIVARGYDLSAQVLKQPHHG
                     YQDVRLQDKPKGRYVYDSDHKYLIDRTGASIAIISNGYKNVNQTPESNVLRDLSGMDV
                     YQTSDKGTIVVSSDGKNLSVSAQKGGNVPSHAGYVVKQKRTPLMQNVTVQANTKKKMT
                     PLRSDASAAYQHYERKNIKIRISAQAKSFTNIKQIQYKFVKKGTSKGSVPYKTGTTLT
                     LKDGMIGRVYVRFVTDAGIDEMQLSGIAVDKKAPTKTKIKVNRSGIKTLKTSTKTSYN
                     KKIKKSAKFVFSASYGISGKSKTQYKIVPKGKKASKYRWKTANKITYKKKNKKVSIYA
                     RFIDKCGNITQRKSSGFYITK"
BASE COUNT         1143 a          482 c          640 g          686 t
ORIGIN      
        1 atggtccatt agagaatgat tatcaatatg gtagaaaggt tgatttgaat acaagacagg
       61 aaaataaata ttctatcgtt acaaagatcg tttacggaag caactctttc ttaatgaccg
      121 gagatgccca gaaagaaacg atcgagaaga tcatcgcgaa gggatatgat ctgacagcac
      181 aggtgctcaa ggagccacat catggatttc aggatgtcac agaagaagag ttagccaatg
      241 gctaccagta ttctgatcat aaaacagtga tcgatgcatc acaggctagt attgcgatca
      301 tcagtaatgg atacatgaat acaggtggtg ttccatatac aaaagtctta agagatttat
      361 caaaactaga cgtctatgag acaggagacc gtggaacgat cattgtaaca tccgatggct
      421 cacagttatc tatccagaca gagaaaggag ataaccagcc aagtgtcaaa ggaaaggaaa
      481 gtgatgatga aacatcatcg ccagtgatga aatccatgaa tatcacagca aacacaacaa
      541 agccattgac agcaacgagt gtggatgctg cgaatactta tgatcgttat gaaaagaaga
      601 atatcacagt aagattttca ggaacagccc aaggatttac aaagctgaca agcattgaat
      661 ataagtttgt gccaaaaggt gtgaataata aaaccatagc atataagaca ggaagttcct
      721 atacagtcaa aaatggaaat tgtggaaggt tttatgtaag atataataca ccattaggat
      781 caacagagat caaacttcct ggatttacag ttgatacgaa agcaccaacc agtgtgaaga
      841 tcaaagcgaa taagtcaggg atcaaaacac tgtcaacatc tgcaaagaat acttacagta
      901 aacgaatcaa gaagtctgtc aaatttacat ttagtgcaaa ttatggaacc agtggaaaat
      961 ccatgacaca gtataagttt gtaccaagag ggaaaaaatc ttccaagtac aaatggaaaa
     1021 caggaaatag tgtaacctat aagacaagga ataaaaaagt tcgtttatat gtaagatata
     1081 ttgataaatc tggaaatatc acaacaaaga agacaaacgg attttatgta actaagaaat
     1141 aacaaagaaa acgctaggat ggaagtatga aatattttac aaagaagatc atagcggtac
     1201 tgctgacagt gatgcttaca ggcatcactg ttttgcacgt atcgggagat caaacgatca
     1261 aagttaatgc agcacagaca tttaaggttc attttatcga tgtgggggcc gctgatggag
     1321 ctttgcttca atatggagaa ggagaaaacg cgaagtatgc cctgattgat tctggggctt
     1381 attcttatga gacaacagat cacgatacga tcgatgtatc agaccgtgtg caccagtatt
     1441 tgctggatca tggagtaaaa catctggaat ttgttgttct tactcatcca catggagatc
     1501 atatcggtgg aatgaaaaag atactagaag ataagaatat tacgatcgat acgatttatg
     1561 gaaatccatt agagtttgaa tatctggaaa gcagcgaaga taaagaaaag caaacagaag
     1621 aaacagcccg atggacagca tttgatacac agacttatca gacattcaag aagaaactag
     1681 agaagaggaa ttcatatcaa gacgcatcat tgcatatcca atatgttgtg ccacaggccg
     1741 gaacatccat aaaattaggg gaagcggtaa tgacttttta tggtccgtta gacaatacct
     1801 ataggtatgg aagagcacag gatgcaccta atttaaatac aagacaggta aataaatatt
     1861 cgatagtgac gaggatcgtt tatggcagta attcattttt aatgacaggc gatgcacagc
     1921 aggaaacgat caaaaagatt gtagctagag gttacgattt aagtgcacag gtattaaagc
     1981 agccacatca tggatatcag gatgttcgtt tgcaggataa accgaaaggt cgttatgtat
     2041 acgattcgga tcataagtat ctgatcgatc gaacaggtgc aagtattgcg atcatcagta
     2101 atggatataa aaatgtaaac cagacaccag agagcaatgt acttcgtgat ctgtcaggaa
     2161 tggatgttta tcagacatca gataaaggaa cgatcgtagt atcgtctgat ggtaagaatc
     2221 taagtgtgtc agcacaaaaa ggcggtaatg taccaagtca tgcgggatat gttgtgaaac
     2281 agaagcgtac ccctttgatg cagaacgtta cagtgcaggc aaatacaaag aagaaaatga
     2341 caccacttcg ttccgatgcc tctgcagcat atcagcatta tgagagaaaa aacatcaaga
     2401 tcaggatttc agcacaggca aaaagcttca caaatataaa acagattcag tacaaatttg
     2461 tgaaaaaagg aacttccaaa ggaagtgtac catacaaaac cggtacgaca ttaacgctca
     2521 aagacggcat gataggcagg gtctatgtga ggtttgtcac agatgcaggg atagatgaga
     2581 tgcagctttc aggaatcgca gtagataaaa aagcaccaac aaagacgaag atcaaggtta
     2641 acagatcagg gatcaagaca ttgaaaacgt caacaaaaac gtcttacaat aagaaaatca
     2701 aaaagtcagc caaatttgta ttttcagcat cctatggaat cagcgggaaa tcaaagacac
     2761 aatataagat cgtgccaaaa ggcaaaaaag catccaaata tcgatggaaa acagcgaata
     2821 agataaccta taaaaagaag aacaaaaaag tgagtatcta tgcaagattt atcgataaat
     2881 gtggaaatat cacacagcga aagagttctg gattctatat tacaaaataa gggaagaaag
     2941 atataagaag g
//
LOCUS       DAOMUE010000259         2946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9602_length_2946_cov_1.829817, whole genome shotgun sequence.
ACCESSION   DAOMUE010000259 DAOMUE010000000
VERSION     DAOMUE010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9602_length_2946_cov_1.829817"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLC71_09585"
     CDS             complement(<1..654)
                     /locus_tag="PLC71_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRM02930.1"
                     /translation="MSLNATPRGDRIHIALFGKRNAGKSSVINAMTNQELAIVSDVKG
                     TTTDPVYKAMELLPLGPVVMIDTPGLDDEGELGEKRVKKAKEVLAKADIALVIMDATA
                     GMTEFEEDMIRLIEDRKIPYLKVYNKMDLSNAQEWKEDKACFVSAKDKKGIWELKEEI
                     GKLVPTDDDTLKIVGDLVCPNDFVILVVPIDSAAPKGRLILPQQQTIRDLLEAGAASI
                     "
     gene            complement(699..1727)
                     /locus_tag="PLC71_09590"
     CDS             complement(699..1727)
                     /locus_tag="PLC71_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HRM02931.1"
                     /translation="MMRVTKKLYALLIAGMMAVSLVGCQSTGNSSNDESTQNEQSSKG
                     STNSSTKSVSSDNIPDFSGNMTVAVDNNDPDFTSKDLTTKSYESYSRLDSEGRCQVAE
                     ACVGKDIMPKGKRGAIGMVKPTGWHTAKYNNVDGKYLYNRCHLIAYQLTGENANNKNL
                     ITGTRSFNVDGMLPYEEMVGDYVRETGNHVLYRVTPVFDGDDLVAKGVQMEAMSVEDK
                     GEDIKFNVFVYNVQDGVKIDYETGDSEADSSVQVTTENSKASQKYHTNQNSSNNSKNY
                     SSNKNTTAAKTNTKTTASQKIRGNSRSKVYHCPGQRDYDRMGTSKYLVTFKSEKEAKA
                     AGYHKAQR"
     gene            complement(1768..2172)
                     /locus_tag="PLC71_09595"
     CDS             complement(1768..2172)
                     /locus_tag="PLC71_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02932.1"
                     /translation="MIIILCLDNNNGMMFNDRRQSQDRGLREYIAEMTKGEKVYMNAY
                     TEKLYEEINDPVVCEDFLHKAGKGETCIVENLPLKPVIDQIEEIVIFRWNKVYPSDQV
                     LDVDLEEWKLAEEEQIEGSTHEITKQIYLPKQ"
     gene            complement(2309..2946)
                     /locus_tag="PLC71_09600"
                     /pseudo
     CDS             complement(2309..2946)
                     /locus_tag="PLC71_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409645.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lyase family protein"
BASE COUNT          657 a          702 c          489 g         1098 t
ORIGIN      
        1 aatagaggct gctcctgctt ctaaaaggtc acggatcgtc tgctgctgtg gaaggatcag
       61 tctgcctttc ggtgctgcac tgtcgatcgg tacgactaag atcacaaaat cattcggaca
      121 aaccaaatct ccaacgatct ttagcgtgtc atcatcagtt ggaaccagtt tcccgatctc
      181 ttcttttaac tcccagattc cttttttatc ctttgcactg acaaagcagg ctttgtcttc
      241 tttccattct tgggcattgg aaagatccat cttattgtag actttcagat aaggaatctt
      301 ccggtcttcg atcaaacgta tcatatcttc ttcaaactct gtcataccag ctgtagcatc
      361 catgatcaca agggcgatat ctgccttagc caagacttct tttgctttct tgactcgctt
      421 ttcccctaat tctccttcat catcaagtcc cggggtatcg atcataacta ctggtcctaa
      481 tggcagaagt tccattgcct tgtatacagg atctgtcgtt gttcctttga catcggatac
      541 gatcgctaat tcctgattcg tcatagcatt gataacgctt gacttcccgg catttcgttt
      601 cccgaataat gcgatgtgaa ttctgtctcc tcttggtgtt gcatttaaac tcatatgatt
      661 tcctcctgtc tgtctttatg gatcgttaac tctttctttt atctttgtgc cttatgatat
      721 cccgctgcct ttgcttcttt ctcactctta aatgtgacta aatattttga tgttcccata
      781 cggtcataat ctctttgacc tgggcagtgg tatacctttg atctgctatt tccacggatc
      841 ttctgtgaag cagttgtctt tgtatttgtt tttgctgctg ttgtattttt gttactgctg
      901 tagtttttgc tgttattaga tgaattctgg tttgtgtggt atttctggct tgcttttgaa
      961 ttctctgttg ttacctgcac gctgctgtct gcttcactgt ctcctgtctc gtaatcaatc
     1021 ttcactccat cctgtacatt atatacaaaa acattaaact taatatcttc gcccttatct
     1081 tccacagaca tcgcttccat ctgtacccct ttggctacca ggtcatctcc gtcaaagact
     1141 ggtgtaactc tgtataatac atgattgcct gtctcacgga catagtcacc gaccatctct
     1201 tcatatggta acattccatc cacattaaaa gatcttgttc ctgtgatcag gttcttatta
     1261 ttggcattct caccggtcag ctggtaagcg atcaaatgac aacggttata tagatatttc
     1321 ccatcaacgt tattgtattt tgctgtatgc catccggttg gttttaccat accgatcgct
     1381 ccgcgttttc cttttggcat gatatctttt ccaacacacg cttccgccac ctgacatctt
     1441 ccttcagagt caagtctgct gtaagactcg taggattttg ttgtcagatc cttggatgta
     1501 aaatctggat cattattgtc aacagcaacc gtcatattcc ctgaaaaatc cggaatattg
     1561 tcagatgata cagatttggt ggaagaattt gtacttcctt tgctgctttg ttcgttctgt
     1621 gtgctttcgt cattactgct attgcctgta ctctggcatc ctaccagtga taccgccatc
     1681 attcctgcga tcagcagggc atataatttc tttgttactc tcatcatcaa ctgtttctcc
     1741 tcttgttata ttcttttctg atctagctta ttgttttgga agatagatct gttttgtgat
     1801 ctcgtgagtg gaaccttcaa tctgttcttc ttctgcaagt ttccattcct caagatccac
     1861 atccagtacc tgatcggacg gataaacttt attccagcgg aagatcacga tctcttcaat
     1921 ctgatctatc actggtttta atggaagatt ctctacaata caagtctccc cttttcctgc
     1981 cttgtgtaag aaatcttcac aaacaaccgg atcgttgatc tcttcataca acttctctgt
     2041 atatgcattc atatagacct tctccccttt ggtcatctcc gcaatatact ctcgcaagcc
     2101 tcgatcctga ctctgccttc gatcattaaa catcataccg ttgttgttgt ctaagcatag
     2161 aattattatc attctttcca tcctccaatc gtacttatgt tctatttatg tattatatct
     2221 tgtgcgagag gatattgcaa taaaagaagc tctgaatttc tccaaagcct cttcttactt
     2281 ttcattcttt tttgtattct aattgtattt atgatactgc tttcatcact ttttctctca
     2341 taggctgtgt cattttctct ggttttaaga tctcttttaa ttcttcttcc tttaacagac
     2401 cttcttctaa taccagctct tttacagaaa ttcctgtttt tagtgatttc tttgcaatct
     2461 cagcagactt tgcatatccg atatatggac ataacgctgt tgagattcca acactttttt
     2521 caatatattc ttcgcactgt ccggcttcgg ctgccatcgt gatcgtatag tcatgtccaa
     2581 tgatcaggta tgcaacctgt gatacaactt ctggaatcac tgagttgatc tttcctggca
     2641 tgatggatga accattctgt cttgctggaa tcatctgcct gttttaatga aattcctgca
     2701 actttgctta attcataaga aatgttcgca agatagtaag ggtctacatt gatcgctgtt
     2761 ccgatcgctg ttgctccgat gtttaagact ttcatctcat ccataacatt tttcagtctc
     2821 ttgatatccc tttttaagac atgtgcaaat gctccaaaac tttgtcctag tctcattgga
     2881 acggcatcct gaagctgtgt tcttcccatt ttgatcacgc cgtcaaactc ttctgctttt
     2941 ttcttc
//
LOCUS       DAOMUE010000260         2924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9691_length_2924_cov_1.934472, whole genome shotgun sequence.
ACCESSION   DAOMUE010000260 DAOMUE010000000
VERSION     DAOMUE010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9691_length_2924_cov_1.934472"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PLC71_09605"
     CDS             complement(<1..1321)
                     /locus_tag="PLC71_09605"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRM02933.1"
                     /translation="MSNVKEIIESGKAYLGIEFGSTRIKAVLIDEEHNPIASGSHTWE
                     NRYENGLWTYSLDDIWAGLQDSYRDMAENVQKEYGATIKKLGAIGFSAMMHGYMAFDK
                     EGKLLAPFQTWRNSNTGKASEVLVDLFQYNIPLRWSIAHLYQCILDKEDHVKDIAFTT
                     TLAGYIHWQMTGEKVLGVGDASGMFPIDMNTKDYDEEMIQKFDKLIEGENYPWKIKEI
                     LPKVLVAGDAAGILTEEGAKKLDPTGNLEAGIPLCPPEGDAGTGMVATNSVAQRTSNI
                     SAGTSFFSMVVLEKPLKDLHTEIDMVTTPDGSLVAMVHSNNGTTDLNAWVNLFKEFAD
                     SIGADVDMNQMYGTLYNKALEGDADCGGILSYGNYAGEPITNVEKGRPMVVRTPETKF
                     NLANFMRSHLYTALGVIKVGMDILTKDEGVEIDTVLGHGGLFKTKGVG"
     gene            complement(1449..>2924)
                     /gene="araA"
                     /locus_tag="PLC71_09610"
     CDS             complement(1449..>2924)
                     /gene="araA"
                     /locus_tag="PLC71_09610"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HRM02934.1"
                     /translation="FWFCTGSQDLYGDECLAHVAEHSKIIVDALNKSGNLPYEVVWKP
                     TMITNEVIRKTFNEANTDENCAGVITWMHTFSPAKSWILGLQEYRKPLLHLHTQFNRE
                     IPYDTIDMDFMNENQAAHGDREYGHIFSRLNMERKVVAGYWEDEDVQKQIGSWMRTAV
                     GVVESSHVRVMRVADNMRNVAVTEGDKVEAQIKFGWEVDAYPVNEVVEAVNAVSQADI
                     DTLVEEYYDKYDILLEGRDEKEFREHVAVQAGIELGFERFLDENNYQAVVTHFGDLGG
                     LKQLPGLAMQRLMEKGYGFGAEGDWKTAAMVRVMKIMTQGMKDAKGTSFMEDYTYNLV
                     SGKEGVLESHMLEVCPTIADGKISIKEQPLSMGNREDPARLVFTSKTGPAIATSLIDL
                     GDRFRLIINDVDCKKTEKPMPKLPVATAFWTPQPNLKVGTEAWILAGGAHHTAFSYDL
                     TAEQMGDWAACMGIEAVYIDKDTTIRQFKNELLWNSVAYRK"
BASE COUNT          660 a          703 c          564 g          997 t
ORIGIN      
        1 gtccgacacc ttttgtcttg aagagtccgc catgtccaag aacggtatca atctcaacac
       61 cttcgtcttt tgttaagata tccattccga ctttgataac accaagtgct gtgtacaggt
      121 gagatctcat gaagttagcc agattgaatt ttgtctctgg tgttctgact accattgggc
      181 gtcctttttc tacatttgtg ataggctcgc ctgcgtagtt accataagaa aggattccac
      241 cacagtcagc atcgccttcc agtgctttat tgtaaagtgt tccatacatc tgattcatgt
      301 ctacgtctgc tccgatagaa tcagcgaatt ctttgaacag atttacccat gcattcagat
      361 ctgttgttcc gttattggaa tgtaccatgg caaccagact tccgtcaggt gttgtaacca
      421 tgtcgatctc tgtatgtaag tctttcagag gtttctcaag gacaaccata gagaagaatg
      481 aagtaccagc ggaaatgttg cttgtacgct gtgctacgct gtttgtagct accatacctg
      541 ttcctgcatc tccttccgga ggacaaagcg ggattccggc ttccagatta cctgtagggt
      601 caagtttctt tgctccttct tctgtcagga taccggctgc gtctcctgct accagaactt
      661 taggaagaat ctcttttatt ttccatggat agttctcacc ttcgatgagt ttatcgaact
      721 tctggatcat ttcttcatca tagtcttttg tattcatgtc gattgggaac attccggatg
      781 catctcctac accaagaacc ttctcacctg tcatctgcca gtggatatat cctgcaagtg
      841 ttgtcgtgaa tgcgatatct tttacatgat cttctttatc cagaatgcac tgatacagat
      901 gtgcgatact ccatctcagc ggaatattat actggaacag atctaccagc acttcggaag
      961 cttttcctgt attgctgttt ctccatgtct ggaatggtgc aagaagcttt ccttctttgt
     1021 caaatgccat atatccgtgc atcatggcac tgaatccgat cgctccgagt tttttaattg
     1081 ttgctccgta ctctttctgt acattttctg ccatatcgcg gtagctgtcc tgaagacctg
     1141 cccagatatc gtccagagag tatgtccaca gtccgttctc ataacggttc tcccatgtat
     1201 ggcttccgga tgcgatcgga ttgtgctcct catcgatcag tactgcttta attcttgtag
     1261 aaccgaattc gattccaaga tatgcttttc cgctttcaat gatctctttt acattgctca
     1321 ttgtacgctt cctcctaaac attatatttt aagttttctt aatatctcca ttatactatt
     1381 tttgcaggga aatatctatt tcgatatccc ctgcaaatat aatcttttac ccgtaattct
     1441 ttatttgatt atttgcggta tgcaacactg ttccacagga gttcattctt gaactgtctg
     1501 attgttgtat ccttatcaat gtatacagct tcgattccca tgcatgctgc ccagtcaccc
     1561 atctgctctg ctgttaaatc ataagagaat gctgtgtggt gagctcctcc tgccaggatc
     1621 catgcttctg ttccaacctt taagttcggc tgtggtgtcc agaatgctgt tgctactgga
     1681 agttttggca ttggtttctc tgtcttcttg cagtctacat cgttgatgat cagacggaat
     1741 ctgtcaccaa ggtcgatcag agatgttgcg atagccggac ctgtcttaga tgtgaataca
     1801 agtcttgcag gatcttctct gttacccatg ctaagtggct gctctttgat gctgatcttg
     1861 ccatctgcga ttgtcgggca aacttccagc atgtgagatt ccagaacgcc ttctttacct
     1921 gatacaaggt tgtatgtgta atcttccatg aatgaagttc ccttagcgtc cttcattccc
     1981 tgagtcatga tcttcataac acgaaccata gcagctgtct tccagtcacc ttctgctccg
     2041 aatccgtatc ctttttccat cagtctctgc attgcaagtc ctgggagctg tttcagtccg
     2101 ccaagatctc cgaagtgtgt tacaactgcc tgatagttat tctcatccag gaatctttca
     2161 aatccaagtt cgattccagc ctgtactgct acatgctcac ggaattcttt ctcatctctt
     2221 ccttcaagaa ggatgtcata tttgtcatag tattcttcta ctaatgtgtc gatatcagcc
     2281 tgagatactg cattaacagc ttctacaact tcatttacag gataagcatc tacttcccat
     2341 ccgaatttga tctgagcttc tactttgtcg ccttctgtta cggctacgtt tctcatgttg
     2401 tcagctacac gcataactct gacgtggctg ctttctacaa caccaactgc tgtacgcatc
     2461 catgatccga tctgtttctg aacgtcttcg tcttcccagt atcctgcaac aactttgcgt
     2521 tccatgttca gacggctgaa gatgtgtccg tactctctgt caccatgtgc tgcctggttc
     2581 tcgttcatga agtccatgtc gattgtgtcg tatgggattt cacggttgaa ctgtgtatgt
     2641 aagtgaagta atggttttct gtattcctgt aatcctaaga tccatgattt tgcaggagag
     2701 aatgtatgca tccatgtaat aacaccggca cagttctcat ctgtgttagc ttcgttgaat
     2761 gtctttctga ttacttcgtt tgtgatcatg gttggtttcc atactacttc atatggaagg
     2821 tttcctgatt tgttcaatgc atctacgata atctttgaat gctctgctac gtgtgcaaga
     2881 cactcatctc cataaagatc ctgtgatcct gtacagaacc aaaa
//
LOCUS       DAOMUE010000261         2907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9769_length_2907_cov_3.472300, whole genome shotgun sequence.
ACCESSION   DAOMUE010000261 DAOMUE010000000
VERSION     DAOMUE010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9769_length_2907_cov_3.472300"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..351)
                     /locus_tag="PLC71_09615"
                     /pseudo
     CDS             complement(193..351)
                     /locus_tag="PLC71_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520505.1"
                     /note="catalyzes the formation of pyridoxal 5'-phosphate
                     from pyridoxal; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
     gene            complement(385..1311)
                     /locus_tag="PLC71_09620"
     CDS             complement(385..1311)
                     /locus_tag="PLC71_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRM02935.1"
                     /translation="MNDFKYVFGPIPSRRLGRSLGISPLPKKTCNYSCIYCQLGRTDK
                     MTNKRQEFYKTEDIIAEFKQYLKDSDKFDIVTVVGEGEPTLAANLGELVVALKALTDK
                     PVAVITNGALLSDPQVREELCHADMVLPSLDAYNQEISKKIDRPYGTIKFEEEFEGLK
                     KFTHMYEGELWLEIMLVDGINDDEQSILKFQELLKELKYDRLYLNTPVRPPAEADVNV
                     VSEERMRYAVETLGGISIEMMSSGAFFSEIEDDYEAVKSIIGRHPMNQFEVRGFLESR
                     DVKDPEAMMEQMKKDEAIHVIDYKGILTFRLK"
     gene            1489..2454
                     /locus_tag="PLC71_09625"
     CDS             1489..2454
                     /locus_tag="PLC71_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRM02936.1"
                     /translation="MKKDKNILVVMPVKDEHKKTLEQHSQGNKFIYSSYDNVTQEMVQ
                     EANVIIGNVDPFYLQEAKNLEWLQLNSAGADPYVKKGVLPEDVILTNATGAYGPSVAE
                     HMLAVLFSLQKKLHLYRDNQNQCEWQDEGGVMSLCGGTLLIVGLGDIGLYFARLMKNF
                     DYHIIGIKRRPGQVPQDIDELHTMDDLDKLLPKADVVLSVLPDSKATRNIFNKDRFEK
                     MKNTSILLNAGRGSAVNTEDLCEALIQGQIYGAGLDVTDPEPLQTQHKLWNVENVIIT
                     PHVAGDFHHPATLYRIVDIIAGNLQRYLEGEQLMNIVDTTTGCKL"
BASE COUNT          900 a          573 c          517 g          917 t
ORIGIN      
        1 cataatctcc tgctcttaaa tcctgtattt cttcctttgc tactggaact gtaatatgtc
       61 tttccatatc cttttccttc ctgattcttt ttctgtattc tgctgcggcg tttttaactg
      121 ctaacaggag tcttataatt ctcttaaaat aatttcagcc agtttctcaa ctgtaaagtc
      181 tacaaaacta aaggtatcaa atcccccagt atgattggaa aggatcgctg tcggcaatgg
      241 acagacctgc actcccatgg atgaaaggat gggaatgatc gctgtcaacg aagctcttcc
      301 aaaaccagac agatcatgaa ttgctgccac tctttttaca ctatgcttca ttttgtttct
      361 cctttttata atccgatcat tgtactattt taaacggaac gtcaggattc ctttgtaatc
      421 gatcacatgg atcgcttcat cttttttcat ctgttccatc atcgcttctg gatctttcac
      481 atctctgctt tctaagaatc ctcgtacttc aaactgattc ataggatgtc ttccgataat
      541 ggatttaacc gcttcatagt catcttcaat ctcactgaaa aatgcaccag aactcatcat
      601 ctcaatcgaa ataccaccaa gtgtctcaac cgcatatcgc atgcgttctt cagatacgac
      661 attcacatct gcttctgctg gcggacgaac tggtgtattt aagtatagtc tgtcatattt
      721 taattctttt aataattctt gaaacttaag aatagactgc tcatcatcgt tgatcccatc
      781 aactagcatg atttccaacc aaagttctcc ttcatacatg tgagtgaact ttttgagacc
      841 ttcaaattct tcttcaaatt tgatcgttcc ataaggtcgg tcgatcttct tggaaatttc
      901 ctggttataa gcatccagag atggcaatac catatcagca tggcataatt cttctctgac
      961 ttgtggatca gaaagcaacg caccatttgt gatcactgcg actggcttgt ctgttaatgc
     1021 tttcagagca actactaatt caccaagatt tgctgctaaa gtaggttctc cttctccaac
     1081 gaccgttacg atatcaaatt tatcagagtc tttgagatac tgtttaaatt ccgctatgat
     1141 atcttctgtt ttataaaatt cctgacgttt atttgtcatt ttatcggttc ttcctaattg
     1201 acagtagatg caggaatagt tacatgtctt cttaggcagt ggactgatac ctaaggatct
     1261 tcctagtctt ctggatggga tcggaccaaa tacatattta aaatcgttca tattgatatt
     1321 tccttttcct attatttttg tataattctt attatacggc acgtatacaa aatttgtcta
     1381 tatgaattgt tctttgaaaa ctcctgaaat ttaatttcta tgggttttct ttctataact
     1441 cttgcgttac aatagtataa aataaacagg aggatattaa gtaattcaat gaaaaaagat
     1501 aaaaacattc tggttgttat gccagtaaaa gacgaacata aaaaaacatt agaacaacat
     1561 tctcaaggca ataaatttat atacagttca tacgataatg taactcagga aatggtacag
     1621 gaagcaaatg tgatcattgg aaatgtagat ccattttact tacaggaagc aaaaaactta
     1681 gaatggctgc agcttaattc agcaggggct gatccttatg tgaagaaagg cgtgcttcct
     1741 gaagatgtaa tattgacgaa tgctacagga gcctatggac caagcgttgc agaacatatg
     1801 ctggcagtct tattttccct gcaaaagaaa ttacatctat acagagataa ccaaaatcaa
     1861 tgtgaatggc aagatgaagg aggtgtcatg tccttatgcg gaggaacact tctgatcgtg
     1921 ggactcggtg acatcggatt atactttgca agattaatga aaaattttga ctaccatatc
     1981 atcggaatca aacgaaggcc agggcaggta ccgcaagata ttgatgaact ccataccatg
     2041 gatgatctgg ataaattgct gcccaaagca gatgttgtat tatctgtact tccagattct
     2101 aaagcaacac gaaatatctt caataaagat cgttttgaaa aaatgaagaa cacctcaatc
     2161 ttgctaaatg ctggaagagg cagtgcagtt aatacagagg atctttgcga agcattgatc
     2221 caaggacaga tttatggagc aggactggat gttacagacc cagaaccgct gcagacgcaa
     2281 cataaattat ggaacgtaga aaatgtgatc ataactccac acgtagccgg agattttcat
     2341 catcctgcga ctttatatcg aatcgtagat attatagctg gaaacctgca aaggtatctg
     2401 gaaggagaac aattgatgaa tattgttgat actacgactg gatgtaaact ctgattgata
     2461 gtgttgcatg tatgagtcag aaagaacaag agaagaaata tttataagaa taaagggtga
     2521 cactccgcat ttttgaaagc gttttttaca aatcgctgat ccgcgcctgt tcctttgata
     2581 acttcctggc tgcgagccaa aatgcccgca gctacggtca gacaatcaac cacagggcgc
     2641 tgcgaatttg taaaaaacgc attcaaaaag tgctcatgtt gtcacccttt tttcttataa
     2701 atatttcttc ttatcttctt tctgactcat acaacgaaac actattcaaa ccactacaca
     2761 gtgtgtatgt taaacggtat tggttgatga tttttgtatt cattgacgga agatgaaaga
     2821 aacttacgaa caccgattta gcgagatgtt ttgaaatata ataaacatct gtctaccact
     2881 acaaaaattg aaaaccaata acgtcta
//
LOCUS       DAOMUE010000262         2905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9776_length_2905_cov_3.763509, whole genome shotgun sequence.
ACCESSION   DAOMUE010000262 DAOMUE010000000
VERSION     DAOMUE010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9776_length_2905_cov_3.763509"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..2076)
                     /locus_tag="PLC71_09630"
     CDS             complement(157..2076)
                     /locus_tag="PLC71_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HRM02937.1"
                     /translation="MTDYNIDFLIAGLVFLIFLLIHFQRYKKLNNISGRTFWFLIIAG
                     IFDIIFDLLSSILLVKSNPAYNSWMIFFSTMLYILQMIVVYIFYNYTQALRHCDEDIR
                     TRNIKIMAVPILCMEVAIVTNVLHKQFFYCNEQGQYVHGKAYLVTYIFTLICIAAVII
                     NCFINQEEYQKNELRAIKEFLGVALVCVTIQMKFQSVLMTGFGLSLGITILFWALYNP
                     QLYIDSLTGLYSKGYFRRWFPEQIKRKKELHLLMIDLWKLKQVNKLYGVTTGDELLIQ
                     IAEYLHKINEANHVFRIHGNRFFVFTTSLMDYETNKDAIMKLFKRPFKVKGERISFSA
                     AICGIINVQEVKEIDVLIDYLEYLVQLKPKSDRTILIQNDQHTKEGFLYEQEVKKYMA
                     TAIEEDLFEVYYQPLYDLKEKRYRTMEALSRLRHPSLGMISPEVFIGIAEKHGQIAKL
                     GYLQFRKVCKFIKEHPQIMEKIESIKYNLSPLELLKSGYSKKLLRTIEEFDIPFSYFQ
                     FEITETVATEYSEALYQTIADFKKSGIQICLDDFGSGYANLNTVLKLPFSTIKLDRSL
                     LNGICYDDQVALLYKNIVAVMQNLGHKVVAEGVETQEEIDLLKQWGVDLVQGYYYTKP
                     LDSEQIIHKLEKDVC"
     gene            complement(2063..>2905)
                     /locus_tag="PLC71_09635"
     CDS             complement(2063..>2905)
                     /locus_tag="PLC71_09635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM02938.1"
                     /translation="FYANALIQGILNKCLKIHMVHMLFVTHVLLFIAVITIVVLMIEE
                     YRRTKDSELKIIMNAFGIMAVAGVLSLCMYWKLEIPFYGTIFEVGVLIFEQLLLTSIF
                     VNLVEQAKTRSELEVYERLLKEDRMTGINNRTAFEEQLQDIEDHAQDYDNAALIFMDV
                     DGLKNTNDLYGHNAGDELIISAALCIKNVFATYGKCYRIGGDEFCVLIFDSIENMDEL
                     LKQMDQEIIKYNRNNRYYLSIARGVSFLKDTEGKQKKISDWKYEADQDMYCNKKRRNI
                     NDRL"
BASE COUNT          896 a          593 c          397 g         1019 t
ORIGIN      
        1 tgacacatga tttcttttta taatattcaa ataatttacg ttattcaaac caccacacag
       61 tgtgtatgtt aaatggtacc gctttgtact atgcattctt tacataaacg ctgttttctt
      121 tcacaaatcc catcttttca agatagcctt tatgtcctaa caaacatctt tctctaattt
      181 atgaatgatc tgctctgaat ctaatggttt tgtataataa tatccttgta caagatcaac
      241 accccattgt tttaacaggt cgatctcttc ctgagtttcc actccttctg ccacgacttt
      301 atgtcccaga ttctgcatga ctgcaacaat atttttatat aataatgcga cctggtcatc
      361 atagcagatt ccatttagca aagagcgatc cagtttgatc gttgaaaatg gtagttttag
      421 taccgtattt agattcgcat atccagatcc aaaatcatct aggcagatct gaattcctga
      481 ctttttgaaa tctgcgatcg tctgatacaa tgcttcacta tactctgtcg caacagtttc
      541 tgtaatctca aattgaaaat acgaaaacgg tatgtcaaac tcttcgatcg tacggagcag
      601 ttttttgcta tatcctgatt ttaataattc caatggagac agattatact ttatactttc
      661 aatcttttcc atgatctgtg gatgttcttt tatgaactta cagacctttc ggaattgaag
      721 atacccaagc tttgcgatct gtccatgttt ctctgcaatt ccgataaaaa cttctggtga
      781 aatcattcca agtgacggat gtctcaatcg gcttaatgct tccattgtac gatatctttt
      841 ttcttttaga tcatacagtg gctgataata tacttcaaaa agatcctctt caattgctgt
      901 cgccatatat tttttaactt cctgttcata aagaaatcct tcttttgtat gttgatcatt
      961 ctgaatcagt atcgttctgt ctgatttagg ttttaactgt accagatatt ccagataatc
     1021 gatcaataca tcaatctctt tgacttcttg aacgttgatg attccacata tcgcagcgga
     1081 aaaagaaatt cgttcccctt ttactttaaa aggtcgttta aatagtttca tgatcgcatc
     1141 tttattcgtt tcataatcca tcaatgatgt tgtgaacaca aaaaaacgat tcccatgaat
     1201 cctgaaaaca tggtttgctt cattaatctt gtgaaggtat tctgcgatct ggatcagcaa
     1261 ttcatctcct gttgtaactc catatagctt attaacttgt tttagtttcc acaaatcgat
     1321 cataagcaga tgaagttcct tctttctctt gatctgctct ggaaaccatc tcctgaaata
     1381 gcctttagaa tacaatccgg tcaggctgtc tatatataac tgtggattat aaagtgccca
     1441 aaataagatc gtaatcccta aggataaccc aaatcctgtc attaatacag attgaaattt
     1501 catctgtatc gtaacacaga ccaatgcaac tccaaggaat tccttgatcg cccgaagctc
     1561 attcttctga tattcttcct gattgataaa gcaatttata atgacagcag ctatacagat
     1621 caatgtaaaa atatatgtta ccaaatatgc ttttccatga acatactgcc cctgttcatt
     1681 acaataaaaa aactgtttgt gtaaaacatt ggtcacgata gctacttcca tacatagtat
     1741 tggtactgcc atgatcttta tatttcttgt tcttatatct tcatcacagt gtcttaatgc
     1801 ctgtgtataa ttatagaaaa tatatacaac gatcatctgc aggatataaa gcatcgtgga
     1861 aaaaaagatc atccatgaat tataagcagg attacttttt accaatagta tggagcttaa
     1921 cagatcaaat atgatatcaa atattcctgc aataattaag aaccagaatg ttcttccact
     1981 gatattattc agttttttat aacgctgaaa atgaatcaac agaaatatta aaaatacaag
     2041 tcctgcgatc aaaaaatcta tattataatc tgtcatttat atttctcctc tttttgttac
     2101 aatacatatc ctgatcggcc tcatatttcc aatcactgat cttcttctgt tttccttctg
     2161 tatcttttaa gaaacttacc cctcttgcaa ttgaaagata atatctgtta tttctattat
     2221 actttatgat ctcttgatcc atttgtttca ataattcatc catattctct attgaatcaa
     2281 aaataagcac acaaaattca tccccgccga tcctgtaaca ttttccatat gttgcaaata
     2341 cattttttat acatagcgcc gcagaaatga tcagttcgtc ccctgcatta tgtccataaa
     2401 gatcgtttgt attttttaat ccatctacat ccataaagat caatgcagca ttatcgtaat
     2461 cttgtgcgtg atcctcaatg tcttgaagct gttcctcgaa tgcagtcctg ttattaatac
     2521 ctgtcatacg atcttctttt agaagtcttt cataaacttc aagctcagat cttgttttcg
     2581 cctgttcaac aagatttaca aaaatactag ttaataacaa ctgttcaaag atcaaaacac
     2641 ccacttcaaa aatggttcca taaaaaggaa tctcaagttt ccagtacatg cataatgata
     2701 atacccctgc aacggccatg atcccaaagg cattcataat gatcttaagt tcgctatctt
     2761 ttgttcttcg gtattcttct atcatcaaaa caacaatggt tattactgca atgaacaaga
     2821 gcacatgtgt tacgaacagc atatggacca tatggatttt caaacatttg tttaaaattc
     2881 cctgaatcaa cgcattcgca taaaa
//
LOCUS       DAOMUE010000263         2904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9782_length_2904_cov_1.898561, whole genome shotgun sequence.
ACCESSION   DAOMUE010000263 DAOMUE010000000
VERSION     DAOMUE010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9782_length_2904_cov_1.898561"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1543
                     /locus_tag="PLC71_09640"
     CDS             137..1543
                     /locus_tag="PLC71_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HRM02939.1"
                     /translation="MSIVYNLDVRCHEMLKYLLYQDGYTSITQLAEEMNISKRSVYYD
                     IRKINEWMEAQHLSELVVERKKGIRIDDEQKAMIRAGLKMIPSELAYVFTPTERVKII
                     ICSILQRNRNLFLDDFMDFCQVSRNTTISDVKEAGRIISEYQLQLIYENGKGYHIKGD
                     LVRKRSIYFSYFSSIADFYKKGILPLDAPEKVQEFLDRLKDIENALDVQYVQGTLYTI
                     AVFFSTIDNRTDVVEFSKKEKMEIMDTKEYEMVSRRFSDLKESEQLYLTLHLLGSRMQ
                     SIPVDFMEEESGQESQWLSRILVKAFSRIAGVGFSKERELTEALAAHLKTSMYRYRYG
                     VQLANPMLDNIKNEYGDLFELTARTCKYLEKEIGFPIPEGEIAYITLHFGAFISAGQE
                     KENRLRILIVCPNGVSTANMIWGEIQTLVPDADVVDVCSLREYERAHDYNVVISTVVI
                     ENEKDLILVHPILTDNDR"
     gene            1561..2190
                     /locus_tag="PLC71_09645"
     CDS             1561..2190
                     /locus_tag="PLC71_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM02940.1"
                     /translation="MKYKHENNVSISNITEIASKYMTNDNLQKFKEELIELFSKNSLK
                     QYVEHKKKPQKGLYEILEDPYIRIIKDKFKWQDAIKVSGEVLLENKLIRQSYIDSIIY
                     KTDQYGPYMFITKQVFLAHSEIEDGAQSMGLSMTIFKKPVEFITLDGKQRFAKIILML
                     SAQDQKSHIRLLNDIMTIFSEQKNEEKLWGQKEILDVKNVLYELLQEEK"
     gene            2187..2636
                     /locus_tag="PLC71_09650"
     CDS             2187..2636
                     /locus_tag="PLC71_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM02941.1"
                     /translation="MKQIYDMLKLENIKILAGVKDWKEAIYTAVQPLVDGGYCEARYS
                     DEIIKNTEKLGPYYVLCENVALIHGSTEQGVIKRQIAITLLKEPVKFKEDGYDVRILV
                     TLAATDAESHMEVLQAMSELFESKESTQRVLDASNEKEIYDLFVGAV"
     gene            2670..>2904
                     /locus_tag="PLC71_09655"
     CDS             2670..>2904
                     /locus_tag="PLC71_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HRM02942.1"
                     /translation="MSFIINILSTPAILVGLISLFGLILQKKPVEDVVKGTVKTIVGF
                     LVLTAGSSFLQTGSLNDFGVIFNYAFNMQGVVPN"
BASE COUNT         1082 a          340 c          601 g          881 t
ORIGIN      
        1 catgagtagt tttgtacaaa ttttgcactg tgtacattct gcagatttgt gctttttctt
       61 tgtacatttc ttggactttt attttaagag ttgattcggt atgataaata tatggaaagc
      121 aaaaggaggt aagaacatgt caatcgtata taacttagat gtaagatgtc atgagatgtt
      181 gaaatattta ttatatcaag atggctatac atcgatcaca caacttgcag aagaaatgaa
      241 tatttctaag cgtagtgttt attatgatat acgcaagatc aatgaatgga tggaggcaca
      301 acatctgtca gaattagtcg ttgagcgaaa aaagggaata cgaattgacg atgaacaaaa
      361 agcaatgatt cgtgcaggat taaagatgat accatctgaa cttgcttatg tgtttacacc
      421 aacagaacga gtaaagatca ttatatgttc tattttgcaa agaaatagaa atttgtttct
      481 tgatgatttt atggattttt gtcaggtgag tagaaataca acgataagcg atgtgaaaga
      541 ggcaggaaga atcatttctg aatatcagct gcagctgatt tacgaaaatg gaaaagggta
      601 tcacattaag ggtgatcttg tacgaaaaag aagtatttat ttttcttatt ttagttcaat
      661 tgcagatttt tataagaaag gaattcttcc gttagacgct ccagaaaaag tacaagaatt
      721 tttggacaga ttaaaagata ttgaaaacgc gttggatgtt caatatgtac aaggaacgtt
      781 atacactatt gcagtgttct tttcaaccat tgataatcgt acagatgttg ttgaattttc
      841 gaaaaaagaa aaaatggaga ttatggatac gaaagagtat gaaatggtat ctagaagatt
      901 ttcagattta aaagaaagtg aacaattata tctgacatta catttactgg gatcaagaat
      961 gcagagtata ccggtagatt ttatggaaga agaatctggg caagaatcac aatggctttc
     1021 caggatactt gtaaaagctt tttcacgaat tgcaggagtt ggattctcaa aggagagaga
     1081 gttaacagag gcactggcag ctcatttaaa gacatcaatg tatcgatatc gatatggagt
     1141 acagcttgcg aatcctatgt tagataatat aaaaaatgag tatggagatc tctttgaact
     1201 cacagcaaga acctgtaaat atttggaaaa agaaattgga tttccgattc cggaaggtga
     1261 aattgcgtat ataacattac attttggcgc atttatcagt gcaggacagg aaaaagaaaa
     1321 cagattaaga attttgatcg tatgtcccaa tggagtcagt actgcaaata tgatatgggg
     1381 tgagattcag acacttgtcc cagatgcaga tgtagttgac gtgtgttcat taagagaata
     1441 tgagagagca catgattata atgtagtgat ttcgacagtc gtaattgaaa atgaaaaaga
     1501 tctgatttta gtacatccga ttcttacaga taatgatcga tgaaagtttt gcaaagatgt
     1561 atgaagtata agcatgaaaa taatgtcagt atttcgaaca ttacagaaat tgcatcaaaa
     1621 tatatgacaa atgataattt acaaaaattt aaagaagaat tgattgagct attttcaaaa
     1681 aattctctga aacaatatgt ggaacataag aaaaaacctc aaaagggatt gtatgaaata
     1741 ttggaggatc catatattcg cattataaaa gataagttta aatggcagga tgcgataaaa
     1801 gtttctggag aggtattatt agaaaataaa ttgatcagac agtcttatat agacagcatt
     1861 atttataaaa cagaccaata tggtccctat atgtttatta caaaacaggt atttcttgca
     1921 cattcagaaa ttgaagatgg agcacaaagc atgggcttat caatgactat ttttaaaaag
     1981 cctgtggaat ttataacatt agatggtaag cagcgatttg caaaaattat tttaatgtta
     2041 tcagctcagg atcaaaaatc gcacattcgt ttattgaatg atataatgac aattttttca
     2101 gaacaaaaaa atgaagaaaa attatgggga cagaaagaaa ttttggatgt taaaaatgta
     2161 ttgtatgaac ttttacagga ggaaaaatga agcagatata tgacatgttg aaattggaga
     2221 atataaaaat tcttgcagga gtaaaagact ggaaagaagc aatttataca gcagttcaac
     2281 cattagtaga tggtggatat tgtgaagcaa gatatagtga tgagatcata aaaaatacag
     2341 aaaaacttgg accatattat gtgctgtgtg aaaatgtggc attgattcat gggagtacag
     2401 aacaaggagt tatcaaacgt cagattgcca ttacattatt aaaagaacca gtaaaattta
     2461 aagaagacgg atacgatgta agaattcttg ttacgttagc tgcaacagat gcggaatcgc
     2521 atatggaagt actacaggcg atgtcagaat tatttgaaag caaagaaagt acgcaaagag
     2581 ttttagatgc ttcaaatgaa aaagaaattt atgacttgtt tgttggggca gtgtagaaat
     2641 tatatggaga tacacaagga ggataaaaga tgagttttat tattaatata ttatcaacac
     2701 ctgcgatttt ggtaggtctg atttcattat ttggtttgat tttgcagaaa aaaccagtag
     2761 aagatgttgt aaaaggaaca gtaaaaacaa ttgtaggatt cttggtactg acagcaggtt
     2821 caagtttttt gcagacaggg tctttaaatg attttggtgt catttttaat tatgctttta
     2881 atatgcaggg agttgtgcca aata
//
LOCUS       DAOMUE010000264         2876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_9913_length_2876_cov_2.747962, whole genome shotgun sequence.
ACCESSION   DAOMUE010000264 DAOMUE010000000
VERSION     DAOMUE010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9913_length_2876_cov_2.747962"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLC71_09660"
     CDS             <1..426
                     /locus_tag="PLC71_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HRM02943.1"
                     /translation="KKEPEVEEESSNSKISQPFDNTITASREAKKRRFTSTGSKVVSM
                     NGRGVEVYVIKPQDFAEAQTAADLLKEGRTVVINLEGVELTVAQRSIDFVGGATYAIN
                     GSLQAVSNNIFIAAPDSIEVSGDLKSEIMNENTISPQLK"
     gene            433..1188
                     /locus_tag="PLC71_09665"
     CDS             433..1188
                     /locus_tag="PLC71_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HRM02944.1"
                     /translation="MKEEELLKKRLMELCEKAYNQNIYTYTHFLNEYEQSIYQDMKQE
                     LAYAHPILVGGHKYFTRAVVMFGSEELFGYQGEVPVVCARISPVFDKFAEELSHRDYL
                     GALMNLGIERHLIGDILIDGRYAYIFCMEHIIGVIKEQLDQVRHTRVFVEEVSWEETG
                     YVQKYKKKEGFVASMRLDVLVSQAFSLSRNISEKLLKSQKVFHNGKMCLSGGQKIKQG
                     DIISVRGYGKFLVEDLGKTSKKGRQFITLAIFQ"
     gene            1198..1788
                     /locus_tag="PLC71_09670"
     CDS             1198..1788
                     /locus_tag="PLC71_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRM02945.1"
                     /translation="MITPVEVLGKELRRGFGYKAVEVDEFLEELAKDYEKVYKENNEL
                     REKVSALTENLSHYRTIEESLKRALVLAEETSKETIENANNKAQALEAEASRNAKQLV
                     SEAEDQAEKILTSAKEDFKEEKEQHEKTIENYQKLIRKLESDYQSYKARMKQFINGQM
                     DILDNPVYEMEFEIAGCEQTDGDEEDEQQKQFGNEQ"
     gene            1778..2857
                     /gene="aroB"
                     /locus_tag="PLC71_09675"
     CDS             1778..2857
                     /gene="aroB"
                     /locus_tag="PLC71_09675"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRM02946.1"
                     /translation="MNNKIEICNEGKKAYDIVLRDSFADLLDQMEFLEIEKKKVCIVT
                     ESNVAPIYLKEVCELISSKASKTITFSFEAGEKHKQLKTIEALYEELIINHFDRQDLL
                     IALGGGVVGDMTGYAAATYLRGIDFVQVPTTLLSQVDSSIGGKTGVDFLQYKNMVGAF
                     HQPKLVYINTTTLKSLPEREYFSGMGEVIKHGLIKDADYYEWIKENIDAIKAREHEAV
                     KEMIYQSCLIKGGVVERDPKEKGERALLNFGHTLGHAIEKLKDFTWLHGECVAAGCCL
                     AAEISCIKGNISKEEVDDIKGVFEAFDMNGDISELSADDIVKTTKSDKKMVGDKIKFV
                     LLRSVGDAYIDMSVTDDEMKQVLQG"
BASE COUNT         1042 a          415 c          706 g          713 t
ORIGIN      
        1 aagaaagagc ctgaagtaga agaagaaagc agtaacagca agataagtca gccgtttgat
       61 aatacaatta cagcaagcag agaagcaaag aaacgaagat ttacaagtac aggaagcaag
      121 gtcgtatcta tgaatggaag aggcgtggaa gtttatgtaa taaagccaca ggattttgca
      181 gaagcacaga cggcagctga tcttttaaaa gaaggaagaa cagttgtgat caatctggaa
      241 ggtgtagaac ttacagttgc acagagaagt attgattttg tgggcggagc aacttatgcg
      301 atcaatggat ccttacaggc agtatcaaac aatattttta ttgcagcacc agacagtatt
      361 gaagtcagcg gagatttaaa aagcgagatc atgaatgaga atacgatctc accacagtta
      421 aaataaaagg tcatgaagga agaagagtta ttaaagaaac gtctgatgga attatgtgag
      481 aaagcatata atcagaatat ctatacatat acacactttc tgaatgaata tgaacagagt
      541 atctatcagg atatgaagca agaacttgct tatgcacatc caatacttgt tggagggcat
      601 aagtatttta caagggcagt ggtgatgttt ggctcagaag aattatttgg ttatcagggt
      661 gaggttccag tggtttgtgc caggatctca cctgtttttg ataaattcgc agaagaatta
      721 agccacagag attatcttgg agcattgatg aaccttggga tcgagagaca tttgatcggt
      781 gatatcctga tcgatgggag atatgcctat attttctgta tggaacatat cataggtgtg
      841 atcaaagaac agcttgatca ggtaagacat acacgagttt ttgtggaaga agtttcatgg
      901 gaagagaccg gatatgtcca aaaatacaag aaaaaagaag gatttgttgc gtccatgagg
      961 ctggatgttc tggtttcaca ggccttttct ttatccagga atatcagtga gaagctgcta
     1021 aagagccaga aagtatttca taatggaaag atgtgtttat caggtggaca aaagatcaaa
     1081 cagggcgata tcatatccgt gcgcggatat gggaagtttc tggtggaaga tttaggaaaa
     1141 acatcaaaaa aaggccgtca gtttatcaca ctggcgatct ttcaatagga ggaatatatg
     1201 attacaccag ttgaggtatt agggaaggaa ctaagaagag gatttggcta caaagcagtc
     1261 gaagttgatg aattcttaga agaattagcg aaagactatg agaaagtcta taaagaaaac
     1321 aacgaactgc gagagaaagt atctgcattg acagagaact taagccatta ccgcacgatc
     1381 gaagaatcct taaagagagc attggttctt gcggaagaga catccaaaga gacaattgag
     1441 aatgcaaaca ataaagcaca ggcattagaa gcagaagcat cccgtaatgc aaaacagttg
     1501 gtatcagagg cagaggatca ggcagagaag atcttaacaa gtgcgaaaga agactttaaa
     1561 gaagagaaag aacagcatga aaagacgatt gagaattatc agaagctgat ccgtaaactg
     1621 gaaagtgatt atcagagcta caaagccaga atgaagcagt ttatcaatgg gcagatggat
     1681 attttagata atcccgttta tgagatggaa ttcgaaattg caggatgtga gcagacagat
     1741 ggagatgaag aagatgaaca gcaaaaacaa tttggaaatg aacaataaga ttgagatctg
     1801 caatgaagga aagaaagcat atgatatcgt attaagagat tcctttgcag acctcctgga
     1861 ccagatggaa ttccttgaaa ttgagaagaa aaaagtctgc attgtgacag aaagcaatgt
     1921 tgcaccaatc tatttaaaag aagtttgtga actgatctca tccaaggctt ccaaaacgat
     1981 cacattcagt tttgaagccg gggaaaaaca taagcagtta aagacgatcg aagcattata
     2041 tgaagaactg atcatcaatc attttgaccg acaggatctg ttgatcgctc taggtggagg
     2101 agttgtcggg gatatgacag gatatgctgc tgcaacatat cttcgaggca ttgattttgt
     2161 acaggttcca acaacacttc tatctcaggt tgacagcagt atcggtggaa agactggtgt
     2221 tgatttctta cagtataaga acatggttgg ggctttccat cagccaaaac ttgtatacat
     2281 caatacaaca accttaaaat ctctgccaga gagagaatat ttctctggaa tgggagaagt
     2341 gatcaaacac ggattgatca aagatgccga ttattatgaa tggatcaaag aaaatataga
     2401 tgcgatcaag gcaagagagc atgaagcagt gaaagaaatg atctatcaaa gctgtctgat
     2461 caaaggcggt gtggtagaac gtgatccaaa ggaaaagggt gagagagcgc tattaaactt
     2521 tggacatact ttagggcatg cgatcgagaa actaaaggat tttacatggc ttcatggaga
     2581 gtgtgtggca gctggatgct gtctggcagc agagatttct tgtataaaag ggaatatcag
     2641 taaagaagaa gttgatgata tcaaaggtgt ttttgaagca tttgatatga atggagatat
     2701 cagtgaactt tcagcagatg atattgtaaa aacaaccaaa tccgataaga aaatggtcgg
     2761 agataagatc aagtttgtat tgttaagatc tgtcggagat gcatatattg atatgagcgt
     2821 gacggatgat gaaatgaaac aagtattaca gggataagtt tttaaggacg agacat
//
LOCUS       DAOMUE010000265         2852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10034_length_2852_cov_2.739006, whole genome shotgun sequence.
ACCESSION   DAOMUE010000265 DAOMUE010000000
VERSION     DAOMUE010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10034_length_2852_cov_2.739006"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..964)
                     /locus_tag="PLC71_09680"
     CDS             complement(89..964)
                     /locus_tag="PLC71_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRM02947.1"
                     /translation="MLAQIQAMFAVVDLGKINWNQFLEKYLEIAMSIIGKVIVSFLII
                     AIGFKLIKILIKLLKTTLEKAEIDYGVISFSCSFIRIGLRCIVIFMAVAHMGVEVSSF
                     IALLGSAGVAVGLALQGTLSNVAGGVLLLILKPFQVGDYIVLTGTDCEGEVAAMDIFY
                     TKLKTIDNRVIVIPNGTLSNSNLINNTKQERRLIDIEVGISYDADIKEVKRILTDIAK
                     NEKRILDQDGVKIFVSSLADSSVMMGIRCWVPTSDYVATKWALNEEIKIRFDEEGIEI
                     PYQKLDVCIKNEVNN"
     gene            complement(1088..2173)
                     /gene="asd"
                     /locus_tag="PLC71_09685"
     CDS             complement(1088..2173)
                     /gene="asd"
                     /locus_tag="PLC71_09685"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRM02948.1"
                     /translation="MSEKLRVGILGATGMVGQRFISLLENHPWFEVTTLAASPRSAGK
                     TYEEAVGGRWKMDTPMPEAVKSMIVKNVNEVEEVASHVDFVFSAVDMSKDEIKKIEED
                     YAKTETPVVSNNSAHRWTPDVPMVVPEINPEHFEVIESQKKRLGTTKGFIAVKPNCSI
                     QSYAPVLTAWKEFEPYEVVATTYQAISGAGKTFKDWPEMEGNIIPYIGGEEEKSEQEP
                     LRLWGHIEDGVIVKAETPVITTQCIRVPVLNGHTAAVFVKFKKKPTKEQLIEKLVNFS
                     GLPQELELPSAPKQMIQYLEEDNRPQVREDVDYENGMGISVGRLREDTVYDYKFVGLS
                     HNTVRGAAGGAVLCAETLKAKGYITKK"
     gene            complement(2184..>2852)
                     /locus_tag="PLC71_09690"
     CDS             complement(2184..>2852)
                     /locus_tag="PLC71_09690"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRM02949.1"
                     /translation="PYVSVRSAWSLSRMVKHIASIDELFETCDYITIHVPYMDSTKGM
                     INKEAIDKMKDGVTLLNFARGELVDNQAVIEGLASGKVKHYVTDFPSAEIAGVDKVIT
                     IPHLGASTEESEENCAEMAVDQLMNYLENGNIVNSVNYPNCDLGEVSGAARITVHHKN
                     LPNMIGQLTAILAADGHNISNMLNKSKGEWAYSMFDMEKKAPTEETIKKMEQIDGVVR
                     VRVL"
BASE COUNT          745 a          632 c          440 g         1035 t
ORIGIN      
        1 actttaaatc atttactggg ctgccgttaa cgacagccct ctccagcacc tgtttgtcca
       61 ggtacgtgca ttgtgcattt acgattcatc aattatttac ttcattttta atacaaacat
      121 ccaatttctg atatggtatc tcgattcctt cctcatcaaa tcgaatctta atctcttcat
      181 ttaacgccca tttggttgct acataatcag atgttggaac ccagcacctg atccccatca
      241 tgacagagct gtctgctaaa ctgctcacaa agatcttaac tccatcctga tcaagaattc
      301 ttttttcatt ttttgctatg tctgtaagaa ttcttttgac ttctttaata tctgcatcat
      361 aagagatccc aacttcaata tcgatcaatc ttcgttcctg ttttgtgttg ttgatcagat
      421 ttgaattgct taatgttccg ttaggaatga cgatcactcg gttatcaatt gtcttaagct
      481 ttgtataaaa aatatccatc gctgcaactt ctccttcgca gtcggttcct gttaatacga
      541 tgtaatctcc tacctgaaat ggtttcagga ttaatagtaa aactccacct gctacattag
      601 aaagtgttcc ttgtaatgct aaacctactg ctacacctgc agatcctaat aatgcgataa
      661 aggagcttac ctctaccccc atatgtgcaa ctgccataaa gattacaata caacgcaatc
      721 caatccttat gaaggagcag gaaaatgaga taactccata atcgatctct gccttctcaa
      781 gggttgtttt taacaattta attaagatct tgatcaattt aaatccaatt gcaatgatca
      841 aaaatgatac tatgactttt cctatgatac tcattgcaat ttccagatat ttttctaaaa
      901 actgattcca gttaatctta cccaagtcca ctactgcgaa catcgcttgt atttgtgcta
      961 acataaactc tcctcttttt ttcttgtgtt tcttttggta ttcattttac acatatgctt
     1021 aacataaaag aaacacgtat ttctacgtgt ttcttatcat agcaagttta tctattcatt
     1081 acaatactta tttttttgta atatatcctt ttgcctttaa tgtctcagca cataaaacag
     1141 ctccacctgc tgctccacga actgtgttat gagaaagtcc tacaaatttg taatcgtata
     1201 cagtatcttc tcttaaacgt ccaacagaga ttcccattcc attttcgtaa tcaacatctt
     1261 ctcttacctg tgggcggtta tcttcttcta agtactggat catctgtttt ggagcacttg
     1321 gaagttctaa ttcctgtgga agtccgctga agtttactaa tttctcgatc aactgttctt
     1381 ttgttggttt cttcttgaat ttaacgaaga ctgctgctgt atgtccgttt aatactggta
     1441 cacggataca ctgtgttgtg atcactggtg tttcagcttt cacgatcaca ccgtcttcaa
     1501 tatgtcccca taaacgaaga ggctcctgtt cgctcttttc ttcttctcca ccgatgtatg
     1561 gaatgatgtt tccttccatc tctggccaat ctttgaaagt ttttcctgct ccagaaattg
     1621 cctgatatgt tgtagcaaca acttcatatg gttcaaattc tttccatgct gttaaaactg
     1681 gtgcataact ctggatagag cagttaggtt ttactgctat gaaacctttt gttgttccta
     1741 aacgtttctt ctggctttcg atcacttcga agtgttctgg gttgatctca ggtactacca
     1801 ttggtacatc tggtgtccag cggtgagcac tgttgttaga tacaactggt gtttctgttt
     1861 ttgcatagtc ttcttcgatc tttttgatct cgtctttaga catatctact gcactaaata
     1921 caaaatctac gtgagaagca acttcttcta cttcattgac gttttttacg atcatagatt
     1981 ttactgcttc tggcattgga gtatccattt tccaacgtcc accaactgct tcttcatatg
     2041 tctttccagc agaacgaggg cttgctgcta atgttgtaac ttcaaaccat ggatgattct
     2101 caagtaatga aataaaacgc tgtccaacca ttccagttgc acctaaaata ccaactctta
     2161 atttttcact cattttttct ctcctatagt acacgaacac gaacgactcc gtcgatctgt
     2221 tccattttct tgattgtctc ttctgttggt gctttctttt ccatgtcaaa catagaataa
     2281 gcccattcac ctttggattt attcaacata ttggaaatgt tatgtccatc tgctgccaag
     2341 atcgctgtta actgaccgat catatttgga agattcttat gatgtactgt aatacgagct
     2401 gctccactta cttctccaag atcacagttt ggatagttta ctgaattgac aatattacca
     2461 ttctctaaat aattcatcaa ctgatccact gccatctctg cacagttttc ttctgattcc
     2521 tctgtagatg ctcctaagtg tggaattgtg atcactttat ctactccagc gatttctgca
     2581 cttgggaaat ctgttacata atgttttact tttcctgatg ctaatccttc gatcactgcc
     2641 tgattatcaa caagttctcc tctggcgaag tttaataatg taactccgtc tttcatctta
     2701 tcgatagctt ctttgttgat cattcctttt gtagaatcca tgtatggaac gtgaattgta
     2761 atataatcac atgtctcaaa taattcgtca atggaagcga tatgttttac cattcttgat
     2821 aagctccatg cagaacgtac ggatacgtat gg
//
LOCUS       DAOMUE010000266         2852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10038_length_2852_cov_1.889882, whole genome shotgun sequence.
ACCESSION   DAOMUE010000266 DAOMUE010000000
VERSION     DAOMUE010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10038_length_2852_cov_1.889882"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLC71_09695"
     CDS             complement(<1..609)
                     /locus_tag="PLC71_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRM02950.1"
                     /translation="MKITLKDGSVKEYENNMSVIDIAKDISEGLARVACAGEVDGEVV
                     DLRTVIDKDCELNILTFDSDEGKHAFRHTASHILAQAVKRLYPETKLAIGPSIDNGFY
                     YDMEKDTPFTQEDLEKIEKEMKKIVKENLEITSFTKPRDEAIAFMKERNEPYKVELIE
                     DLPEDSVISFYQQGEFVDLCAGPHLMTTKPVKAFKLTSIAGAY"
     misc_binding    complement(728..951)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1014..>1642)
                     /locus_tag="PLC71_09700"
     CDS             complement(1014..>1642)
                     /locus_tag="PLC71_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRM02951.1"
                     /translation="SKYVFGINHPDFETLLDKNETIQLDQYLKNEQILPIKPMEEKVQ
                     TIKKQIKKFKGEIKKEEQTRYFNPNDVSALSHITVSDAKHILEGTALYDDAKAYVKAE
                     EKYHVNAVFLMGIAAHESAWGTSRRAREDNNLTGYGVTSDHAKGINKSTKEAGLLATA
                     ETLHEKYLTSGGSYYVGTSAAAVNKHYCVGGEWAAAVVKNAYLLMNRL"
     assembly_gap    1643..1683
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1684..2172)
                     /locus_tag="PLC71_09705"
     CDS             complement(<1684..2172)
                     /locus_tag="PLC71_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HRM02952.1"
                     /translation="MRRKIMTAFVCAIMTISVIPVYADDTQTDQAVTTEVSTETAATV
                     QETKRTQSKEQQKKKTTSKKHTKTKKTKLKKKEENKVIPMPTQVISKSSSDTIEGRIK
                     DKQAKVKQLKKQKAKIEEEIRLEQQKARSINEFYKKYTANSKAEIALYGQEITPSMKI
                     SKK"
     gene            complement(2356..2841)
                     /locus_tag="PLC71_09710"
     CDS             complement(2356..2841)
                     /locus_tag="PLC71_09710"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRM02953.1"
                     /translation="MKLEQFYYQIYIDYHDKEWGVPVYDDHKLFEMLILESFQAGLSW
                     ECVLNKREAFREAFDDFDVYKVSAYDEEKQAQLKENKGIIKNQRKISAAVSNATIFMK
                     IQEQYGTFSEYLWKFTGHKIIHETGKTSSELSDQISKDLKKRGMKFVGTTIIYSYLQA
                     V"
BASE COUNT          712 a          607 c          426 g         1066 t
ORIGIN      
        1 gtaagcacct gcaatacttg taagtttgaa tgctttcact ggttttgtag tcattaagtg
       61 aggtcctgca caaagatcga cgaattctcc ctgctgataa aagctgatca cagaatcttc
      121 tggaagatct tcaatcaatt ctactttgta aggttcgttt ctttctttca taaatgcaat
      181 cgcttcatct ctaggttttg taaagcttgt gatttcaaga ttttctttga cgatcttctt
      241 catttctttt tcaatcttct caaggtcttc ctgtgtaaat ggagtatctt tctccatatc
      301 atagtagaaa ccattgtcga tagaaggtcc gatcgcaagt tttgtttctg gatataaacg
      361 ttttactgcc tgagctaaaa tatgggatgc tgtatgacgg aatgcgtgtt ttccttcatc
      421 agaatcaaat gttaagatat ttaattcgca gtctttgtca atgacagttc taaggtcaac
      481 aacttctcca tcaacctcac ccgcacaggc aactcttgcc agtccttcac tgatatcttt
      541 tgcaatatca atgacagaca tattgttttc atactctttt acagatccat cttttaatgt
      601 aatcttcatc ttcttattca tcctttcttg atatataacg ttttatttat cctgtcacaa
      661 ttctctatag atttcttcta taataaatct ataacagaaa taaaaaatcc catggactaa
      721 taaaaatcca tgggacgagt tatcgcggtt ccaccctggt tatctgtttc atatcctttg
      781 atcataaact ccagaaacag atcacttaat cgacgattaa cggtgtcagc cgggctggtt
      841 tacagccact ccgaggtggt cttcctttgt ttgtctgctt aggatacttt cagcaaatgt
      901 atccctctct ggaagctttc cacaaagtac tcttctcatc aacgttttct tctttttgat
      961 ttcttgtaaa ttatagcatt gtgattctat aaaatcaata caaaaataga aaattataaa
     1021 cgattcatta ataaataagc attctttaca actgctgccg cccattctcc gccaacacag
     1081 taatgtttgt tcactgctgc cgcagaagtt ccaacatagt aacttccacc tgatgtcagg
     1141 tatttttcat gtaaagtttc tgcagttgct aaaagtcctg cttctttcgt tgatttgtta
     1201 attccttttg catgatccga tgttacacca tatccagtca gattattatc ttccctcgca
     1261 cggcggctcg tgccccatgc actttcatgt gcagcgattc ccatcaaaaa gactgcattc
     1321 acatgatatt tttcttccgc cttgacataa gctttcgcat catcatacaa agcagttcct
     1381 tccaaaatat gtttggcgtc tgaaactgtg atatgactta gagcactgac atcatttgga
     1441 ttaaaatatc ttgtctgttc ctcttttttg atctctcctt tgaacttctt gatctgcttc
     1501 ttgatcgtct gaaccttttc ttccattggt ttgatcggaa gtatctgttc gttctttaaa
     1561 tactgatcaa gctgaatcgt ttcattctta tcaagcaaag tctcaaagtc cggatgattg
     1621 attccaaaga catatttgct tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnntttttta gaaatcttca tagaaggagt gatctcctgc ccatataaag caatctccgc
     1741 cttggaattt gctgtatact ttttgtaaaa ttcatttata gatcgtgctt tttgctgttc
     1801 caggcgaatc tcttcctcaa tctttgcttt ttgttttttc agctgtttca ctttcgcctg
     1861 tttatccttg atccttcctt cgatcgtatc cgaagaactc ttactgatca cttgtgttgg
     1921 cattggaatg actttgtttt cttccttttt ctttaatttt gtcttctttg tcttagtatg
     1981 tttcttgctt gttgtctttt tcttctgttg ttctttactt tgcgttcttt ttgtttcttg
     2041 caccgttgct gctgtttcgg tagaaacttc tgttgtcact gcctgatcgg tctgtgtatc
     2101 atctgcataa accggtatta cagagattgt cataatcgcg catacaaatg ctgtcataat
     2161 cttacgtctc atgatcgctt cctccctttg tttgaacatt gttatgacat gatcatcttt
     2221 ctaatactca attttatctg gtaatttcta ccttactcct ttttgtataa aaattcaagt
     2281 acgcagtgtt acgtacttgt aaatttttta ttctttatga tgcaggaaac aaccttcctc
     2341 atgtgactcg accactcaca ctgcctgcag ataggaataa atgatcgttg taccgacaaa
     2401 tttcattcca cgtttcttta aatcctttga aatctggtcg gataattctg aagatgtttt
     2461 tcccgtctca tggatgatct tatgacctgt aaacttccat aaatattcag aaaaggttcc
     2521 gtattgttcc tggattttca taaaaatagt cgcatttgat acggcagcag atatttttct
     2581 ttggtttttt ataatccctt tattttcttt taattgcgct tgtttctcct catcatatgc
     2641 actcacttta tatacatcaa aatcatcaaa cgcttctctg aacgcttctc gtttatttaa
     2701 cacacactcc cacgataatc ctgcctgaaa cgattccaag atcagcattt caaacaattt
     2761 atggtcgtca tagacaggca ctccccattc tttatcgtga taatcaatat atatctgata
     2821 ataaaactgc tccaatttca aatgtaacgg ca
//
LOCUS       DAOMUE010000267         2849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10050_length_2849_cov_3.317108, whole genome shotgun sequence.
ACCESSION   DAOMUE010000267 DAOMUE010000000
VERSION     DAOMUE010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10050_length_2849_cov_3.317108"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..883)
                     /gene="sdaAA"
                     /locus_tag="PLC71_09715"
     CDS             complement(20..883)
                     /gene="sdaAA"
                     /locus_tag="PLC71_09715"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HRM02954.1"
                     /translation="MGFASVEEIETRAKEQECQLWETILHDDMTERQVDRLESIGKMS
                     SMYLAMKDANESYDKDLKSQSGLSGGDGKKMMEEVRKMQNLTGEFVGTVMANALKMGE
                     SNACMKRIVAAPTAGACGVLPAVLITYEQFHKVPEAKMLEGMYIAAGVGQVIAERACI
                     AGAQGGCQAEIGSASCMAATAITYIRGGSTKQIFDAGAFALKSLLGLVCDPLGGLVEV
                     PCIKRNVIGSVNAITASDMAMAGIESKVPLDEVIDAMAEVGDLLPCSLKETSQAGLAQ
                     TETGKKYMPES"
     gene            complement(887..1555)
                     /gene="sdaAB"
                     /locus_tag="PLC71_09720"
     CDS             complement(887..1555)
                     /gene="sdaAB"
                     /locus_tag="PLC71_09720"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HRM02955.1"
                     /translation="MNLFDIVGPVMVGPSSSHTAGAVRIGQVSRHLLGESVKEAKILL
                     HGSFLATGKGHGTDKALVAGLLGMAADDIRIPDSFDIAKESGVKFEIGKIQLKNAHPN
                     SVKLLLVGESGRNLEIVACSLGGGRIKICTIDGLEANFSGEYTTLVVHNDDQPGHVAE
                     VTSMLAHKSVNIATMQLYRDHRGGSAVMVIECDKEIPEEGLNFLERLEGIQKVTYLAK
                     MKED"
     gene            complement(1723..>2849)
                     /locus_tag="PLC71_09725"
     CDS             complement(1723..>2849)
                     /locus_tag="PLC71_09725"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRM02956.1"
                     /translation="SWNKGFKEYFAVKATPNPTILKILKEEGCGTDCSSLTELMMSDR
                     CGFDGSEIMFSSNDTPAEEFILAKQLGATINLDDITHIEFLKECAGIPEKISCRYNPG
                     GVFALGTDIMDNPGDAKYGMTTEQIFEAFKQLKELGVKEFGIHSFLASNTVTNEYYPT
                     LAKQLFELAVKLKEEVGVHIGFINLSGGVGIPYLPDQEGNDIAVIGEGVHKVYDEVLV
                     PAGMGDVKIFTELGRYMLAPNGHLVTKAIHEKHTHKEYIGVDACAVNLMRPAMYGAYH
                     HITVMGKENEPADHVYDITGSLCENNDKFAIDRKLPKIDMGDLLVIHDTGAHGFAMGY
                     NYNGKLKSAEILLKEDGTTEMIRRAETPEDYFATIKGFNF"
BASE COUNT          698 a          663 c          484 g         1004 t
ORIGIN      
        1 tcctttactt acaaatattt tatgattccg gcatgtattt tttgcctgtt tctgtctggg
       61 caagacctgc ctgactggtt tcctttaaag aacatggcaa gaggtctccg acttctgcca
      121 ttgcatcgat cacttcatcc aatggaacct tgctttcaat gcctgccatt gccatatctg
      181 aggctgtgat cgcattgaca gatccaatga catttctctt gatacatggt acttctacca
      241 aaccacctaa tggatcacat accaatccca ataatgattt caaggcaaat gctcctgcat
      301 caaagatctg ctttgtgctg ccgccacgaa tgtacgtaat agctgttgct gccatacagg
      361 aggcagaacc gatctctgcc tgacatcctc cttgtgcccc tgcgatacag gcacgttctg
      421 cgataacttg tccgactcct gccgcaatat acattccttc tagcatcttt gcttctggta
      481 ctttatgaaa ttgttcatat gtgatcaata ctgctggaag caccccacat gctccggctg
      541 tcggtgctgc tacgatacgt ttcatacagg catttgattc gcccattttt aaagcatttg
      601 ccatgacggt cccgacaaat tcaccggtca gattctgcat ttttctaacc tcttccatca
      661 tctttttacc gtcgccgcca gaaagaccgc tttgtgactt taagtcttta tcgtaacttt
      721 cattggcatc tttcattgca agatacatac tgctcatttt cccgatagac tctaggcggt
      781 ctacctgtcg ttctgtcata tcatcatgta aaattgtttc ccataattga cattcctgtt
      841 cttttgctct tgtttctatc tcttctacgg atgcaaatcc catactctag tcctccttca
      901 tcttagcaag atatgtaact ttctggattc cttccagacg ttccaaaaag ttcagacctt
      961 cttctggtat ttctttgtca cattcaatga ccatgactgc acttccacct ctgtgatcac
     1021 ggtataattg catcgttgcg atattaaccg atttatgagc aagcattgat gttacttctg
     1081 ctacatgtcc tggctggtcg tcattgtgta cgactaatgt tgtatactcc ccgctaaagt
     1141 ttgcttccag tccatcaatc gtgcagatct taattcgtcc accgcctaag gaacaggcta
     1201 caatctcaag attccttcct gattctccga caagtaataa ttttacactg tttggatgtg
     1261 cattttttaa ttgaatcttt ccaatctcaa atttcactcc tgattctttt gcaatgtcaa
     1321 aactgtctgg aatccgaatg tcatcggctg ccatgccaag taatcctgcc actaatgctt
     1381 tgtctgtacc atgtcctttt cctgttgcaa gaaatgatcc atgtaatagt attttagctt
     1441 cttttaccga ttctccgagc aggtgccttg atacctgtcc gatccttaca gcacccgctg
     1501 tatgagagct cgacggtccg accatcactg gtcctacgat gtcaaataaa ttcatgtgat
     1561 ttctcctgtc tataaatgcc tttcgtcttt tgaaatgctg ttttgccgtg tatcattttg
     1621 tttcttgtat ataatttact cgtaatatat gaaaaaggca agtatagaat ttcctatact
     1681 tgccctgctt tggaacatct catattgatc tgttaatttt tcttagaaat taaatccttt
     1741 gatcgttgca aaataatctt ctggtgtttc tgcacgacgg atcatttctg ttgttccatc
     1801 ctcttttaat aagatttctg cagattttaa tttaccatta tagttatatc ccattgcaaa
     1861 tccgtgtgct cctgtatcat gaatcactaa cagatctccc atgtcgatct ttggtaattt
     1921 acggtcgatc gcaaatttat cgttattttc acaaagagat cctgtgatat catatacatg
     1981 atctgctggt tcgttttctt tacccataac tgtgatgtga tggtacgccc catacattgc
     2041 aggtctcatt aagttgactg cacatgcatc aacaccaatg tattctttat gtgtatgttt
     2101 ttcatggatt gcttttgtta ctaaatgtcc gtttggtgca agcatgtaac gtccaagttc
     2161 ggtaaagatc ttcacatctc ccattccggc tggtactaat acttcgtcat atactttgtg
     2221 tacaccttca ccaatgacag cgatgtcatt accttcctga tctggaaggt aagggattcc
     2281 gactccacca gataagttga taaatccgat atgaactcca acttcttctt ttaatttaac
     2341 tgctaattca aataactgtt ttgcaagtgt tggatagtat tcatttgtaa ccgtgttact
     2401 tgcaaggaaa gaatggattc cgaattcttt gacaccgagc tcttttaact gtttgaaagc
     2461 ttcaaagatc tgttctgttg tcattccgta ttttgcatct cctgggttat ccatgatatc
     2521 tgttcctaat gcgaaaactc cacctggatt gtaacggcag gaaatctttt ctgggattcc
     2581 ggcacattct tttaagaatt cgatatgtgt aatgtcatct aagttgatcg ttgcacctaa
     2641 ctgttttgcc agaataaatt cttctgctgg tgtatcgtta gaagagaaca tgatctcgct
     2701 tccatcgaat ccacaacgat ctgacatcat aagttctgtt aaagatgagc agtctgttcc
     2761 gcatccttct tcttttaaaa tctttaagat tgttggattt ggtgtcgctt ttacagcaaa
     2821 atattcttta aatcctttat tccatgaaa
//
LOCUS       DAOMUE010000268         2844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10083_length_2844_cov_2.220868, whole genome shotgun sequence.
ACCESSION   DAOMUE010000268 DAOMUE010000000
VERSION     DAOMUE010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10083_length_2844_cov_2.220868"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1034
                     /locus_tag="PLC71_09730"
     CDS             192..1034
                     /locus_tag="PLC71_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HRM02957.1"
                     /translation="MRKGTLKKALALALTGAVLVGAFAGCGAKKDSGDKKETKKIVIG
                     ASPSPHADILKVAKKELKKEGYELEIKEYSDYVQPNTALESGDLDANYFQHKPYLDDF
                     NKQKKTHLVSAGTIHYEPFGIFPGKTKTLKALKNGATVAVPNDTTNEARALLLLQDQG
                     LIKLKDGAGLTATKKDIVENKKDLAIKEIEAAQIPRSLKDVDIAVVNGNYALEAGLKV
                     NKDALATEDADSIGAKTYGNVVAVKKGNEKTDATKALIKALKSDTVKKYINDKYDGAV
                     VPLF"
     gene            1232..2242
                     /locus_tag="PLC71_09735"
     CDS             1232..2242
                     /locus_tag="PLC71_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM02958.1"
                     /translation="MIELKNIDVTFQNGSHEFKAVDNVSLKINKGEIFGIVGPSGAGK
                     SALVRVINLLQRPTNGEVIIEGSDITKFNRKQLRKTRLNIGMIFQHFNLISGSTIAEN
                     VAFSLYANNYPKDKIKDRVKELLEIVHLPEKADAYPANLSGGQKQRVAIARALANNPD
                     ILLCDEATSALDIENTEEIVELLREINEKTHITIVFITHEMDVAKKLFDRIAFMEDGK
                     LKEVTDIYSAFANPKSDIAKSLVQRVLNVEVPEGLKAKTNEEILRLSYTGDDSYDPVI
                     SVASKNYDITMDIINGKVDYIRNKPFGMLIVILRGEDAERKKALEYISKNVYKLERLG
                     GR"
     gene            2244..>2844
                     /locus_tag="PLC71_09740"
     CDS             2244..>2844
                     /locus_tag="PLC71_09740"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HRM02959.1"
                     /translation="MNETLEIIQSEFAQASFETFEMVLISMIAAAILGTILGLVLHLA
                     SNPIFYKNRVVNGITGTIINVIRSLPFIILIIVLQPLARLVVGTSIGPIAASVSLAIA
                     AIAFYARLVEGAFSEVDKGVIEAAIATGASMPLIIRKVLFVEATPALVRALTVTFVSV
                     IGYSAMAGAVGGGGIGNLAIMYGYNRYQTGVLLVTVVALV"
BASE COUNT         1085 a          421 c          602 g          736 t
ORIGIN      
        1 tgggtgagat atcagtttta aaagaaagct gcattattta tatacaaaca atctgctata
       61 gccaaaaact atatcaaact aaaagaaata catattttac aaaatcctaa tacactgtta
      121 tactagactc aatcagttga aacaatacaa tataaataac aaaataatag aaagagtacg
      181 aggtgaaaag tatgagaaaa ggaacattaa aaaaagcatt agcattagca ctgacaggag
      241 cagttttagt cggagcattc gcaggatgcg gagcaaagaa agatagcggt gataaaaaag
      301 aaacaaagaa gatcgttatt ggagcaagtc catctccaca tgctgatatc ttaaaagtag
      361 cgaagaaaga attaaagaaa gaaggatacg aattagaaat taaagaatac tctgattacg
      421 tacagccaaa tacagcactc gaatctggag atttagatgc aaactatttc cagcacaaac
      481 catatttaga tgacttcaac aaacaaaaga agacacattt agtatccgct ggaacgattc
      541 attatgaacc atttggaatc ttcccaggaa agacaaagac attaaaagca ttaaagaacg
      601 gtgcaacagt tgcagttcct aacgatacaa caaatgaagc aagagcgtta ttattgttac
      661 aggatcaggg acttatcaaa ttaaaagacg gagcaggatt aacagcaaca aagaaagata
      721 tcgtagagaa caaaaaagat ttagcaatca aagaaatcga agcggctcag attccaagat
      781 cattaaaaga tgttgatatc gcagttgtga atggaaacta tgctttagaa gcaggattaa
      841 aagtcaacaa agatgcatta gcaacagaag atgctgattc tatcggagct aaaacatatg
      901 gaaatgttgt agcagtgaaa aagggaaatg aaaaaacaga tgcaacaaaa gcactgatca
      961 aagctctaaa gagtgataca gtgaaaaaat atatcaatga caaatatgat ggagctgttg
     1021 tacctctgtt ctaagagcag aagattgcta taaaccataa ataatttatg gaagtaacaa
     1081 gaaattattg aaaaaatcaa ctgaaattgt tatgatgaag ctattgtctg tcaggaaaac
     1141 tgataggcga tagcttatct gattttacag gataagaaaa aagtccgatg aagtgatcaa
     1201 aggacaatga aaaataagaa aggataaagt catgattgaa ttaaaaaata tagatgttac
     1261 cttccaaaat ggcagtcatg aattcaaagc agttgataat gtaagcttaa agatcaacaa
     1321 aggagagatt tttggaatcg taggaccaag tggtgctgga aagagtgctc tggtacgtgt
     1381 gatcaatttg ttacagagac caacgaatgg agaagtgatc attgaaggat cagatattac
     1441 gaagtttaac cgaaagcaat taagaaaaac aagattgaat attggaatga tcttccagca
     1501 ttttaatctg atcagtggat caacgatcgc tgaaaatgtt gcattttcat tgtatgccaa
     1561 taactatcca aaggataaga tcaaagacag agtgaaagaa ctcttagaga tcgtacatct
     1621 tccagaaaaa gcagatgcat atccagcaaa cttaagtgga ggacagaaac aaagagttgc
     1681 gattgcaaga gcgttagcga acaatccaga tatcctgtta tgcgatgaag caacctccgc
     1741 attggatatt gagaatacag aagaaatcgt ggaattatta cgagagatca atgaaaaaac
     1801 acatatcacg atcgtattta tcacacatga gatggatgta gcaaagaaat tatttgatcg
     1861 tattgcattt atggaagatg gaaagctgaa agaagttaca gatatctatt ctgcctttgc
     1921 aaatcctaag tcagatattg caaagagtct ggtacaacgc gtattaaatg tagaggttcc
     1981 agagggatta aaagcaaaaa caaatgaaga gatccttcgt ttaagttata caggagatga
     2041 ttcttatgat cctgtgatca gtgttgcttc taagaactat gatattacaa tggatatcat
     2101 caatgggaaa gtcgattaca tcaggaataa accatttggt atgttgatcg taatccttag
     2161 aggagaagat gcagaacgta agaaagcatt agaatacatt agtaagaatg tatataaatt
     2221 agagaggtta ggaggaagat aatatgaatg aaacattaga gatcatccag tcagaatttg
     2281 cccaagcatc ttttgagacc tttgagatgg tattgatctc catgatcgca gcagcaatcc
     2341 ttggaacgat cttaggacta gtattacatc tggcatcgaa tccgattttt tataaaaaca
     2401 gggtagttaa tggcattaca ggaacgatca tcaatgtgat caggtctttg ccatttatca
     2461 ttttgatcat tgtattacag ccgttggcaa gactggttgt aggaacaagt atcggaccaa
     2521 tcgcagcatc tgtatctttg gcaatcgcag caatcgcatt ctatgcaaga cttgtggaag
     2581 gtgcatttag tgaggttgat aagggtgtga tcgaagcagc gattgcaaca ggagcaagta
     2641 tgcctcttat tattcgtaag gttttatttg tagaagcaac accagcactg gttcgtgctt
     2701 taactgtaac ttttgttagt gtgatcggat attcagcaat ggctggagct gttggaggcg
     2761 gcgggatcgg taatcttgcg atcatgtatg gatataaccg ctatcagaca ggagtattat
     2821 tagtaacagt tgttgcactt gtca
//
LOCUS       DAOMUE010000269         2837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10130_length_2837_cov_1.759526, whole genome shotgun sequence.
ACCESSION   DAOMUE010000269 DAOMUE010000000
VERSION     DAOMUE010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10130_length_2837_cov_1.759526"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLC71_09745"
     CDS             complement(<1..189)
                     /locus_tag="PLC71_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRM02960.1"
                     /translation="MKAIILNQPKDFSIKEIDKPRIKDDEVLIRVKASGICTNDVRDF
                     NGDCNYSYPRIGGHEYGGV"
     gene            complement(251..1093)
                     /locus_tag="PLC71_09750"
     CDS             complement(251..1093)
                     /locus_tag="PLC71_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HRM02961.1"
                     /translation="MENNKKKSLIPKAALIKAWLIWETFPQTCYNYERMMGQVVAHMF
                     SAIIGFLYKDDPSKRKEVMKREIEFFNVHIEFGSCILGMAVALEEQKAMGENIPGEFI
                     TNLKTSLMGPLAGMGDTIWQGVVIPILLAVCIDLTRSGSGNVWGAVIYAVVIVVAAYA
                     LSYWNFMFGYKAGSEAIMDFLEKGILNKLIKGASIMGCMVMGGLIVNYVKAVCGLKIV
                     SSTTTYSIQTNFLDQVCPSILPLAVTLLVYYLMNKKRWSSLKIIGLIVVIGIVGGVTG
                     ILAY"
     gene            complement(1095..1889)
                     /locus_tag="PLC71_09755"
     CDS             complement(1095..1889)
                     /locus_tag="PLC71_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HRM02962.1"
                     /translation="MEMTILQAVLCGVVYWLAVGNLPFVGLWSLQRPLVCGAITGLIL
                     GHPVQGAVIGATINLVYLGFMSAGGSMPADMGLAGVLGTAYAIVGGLDTDTALALAVP
                     IGILGTIVWAGRMTFDCFFVHMADKYIEEEKYNKIWIANVLLPQILCFCMTAIPCALA
                     AYFGSSYIQGILAMLSGKVLTVFQIIGGLMPALGIAITLQYIFKGESKVFLFIGFLLA
                     VYSKLPLLTLGIIALLVAVVYVQVTSAVQPATAGADYDDEDDEEDD"
     gene            complement(1914..2399)
                     /locus_tag="PLC71_09760"
     CDS             complement(1914..2399)
                     /locus_tag="PLC71_09760"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HRM02963.1"
                     /translation="MKNIVLARVDDRLIHGEVVSVWTPSLNVNRIIVVDDEVAADKFN
                     KRVIKALAPNGVKVNVYGTEKGADILQRDSKDPNERVMILTKTPITYDRMADMGLKLS
                     DVNLGGMGLRGERTPFIKNVACSPDEITSIKNLRAKGVHVYYQLVPEQQVIEVDGYLN
                     E"
     gene            complement(2396..2812)
                     /locus_tag="PLC71_09765"
     CDS             complement(2396..2812)
                     /locus_tag="PLC71_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM02964.1"
                     /translation="MMKVIAVSHGSYSKGLVDSVQMLVGEQESLVAYGLFPEQTVATL
                     TEKLEKEINDTPEGEEILFLTDLFHGSPFNAVVSLMRDHEFHHITGINIPLAVEVMMG
                     RYADKSAEEICRGIMEAAPDTVKYVNDLFDDEEEEE"
BASE COUNT          814 a          668 c          422 g          933 t
ORIGIN      
        1 cactccacca tattcatgtc caccgattct tggataactg taattacaat ctccattaaa
       61 atctctgaca tcatttgtac agattccaga tgctttgaca cgaatcagta cttcatcatc
      121 tttgatccgt ggtttatcga tttctttaat tgaaaaatct tttggctgat ttaaaataat
      181 tgctttcata gctctcccac actttctttt tagtatcgaa gggaagcaaa tgctcccctt
      241 ctcgtaattt ctaatatgct aagattcctg ttacaccacc gacgataccg atcactacga
      301 tcagtccaat gatttttaag gaagaccaac gttttttgtt catcagatag taaactaata
      361 atgttactgc caatggtaaa atacttgggc aaacctgatc caagaagttt gtctgaatgc
      421 tgtaagttgt tgtagaactt acgatcttta atccacatac agcttttaca tagttaacga
      481 tcagaccacc cattaccata caacccatga tagaggcacc tttgatcagt ttatttaaga
      541 ttcctttttc aaggaagtcc atgattgctt cacttccggc tttataacca aacatgaagt
      601 tccagtaact taatgcgtat gctgctacta cgatgacaac tgcatagatc acagctcccc
      661 atacatttcc acttccggaa cgtgttaaat cgatacatac tgctaataag atagggataa
      721 ctacaccctg ccagatcgta tcacccatac cagctaaagg tcccattaaa gaggtcttta
      781 agtttgtaat aaattcaccc ggtatattct cgcccatcgc cttttgttct tccaatgcga
      841 ctgccatacc taagatacaa gacccaaatt caatatgtac gttaaaaaat tcaatctcac
      901 gtttcataac ctctttacgt tttgaagggt catctttata taagaaacca atgatcgctg
      961 agaacatatg ggcaacaacc tgtcccatca ttctctcata gttgtaacat gtctgtggaa
     1021 atgtctccca gatcagccag gctttgatca gggcggcctt cgggattaaa cttttctttt
     1081 tattgttttc catcttaatc gtcctcctca tcatcttcat catcataatc tgcgcctgct
     1141 gtcgctggct gtactgctga agttacctgc acatatacaa ctgctactaa tagtgcaatg
     1201 atacctaatg ttaataatgg taacttagaa tataccgcta ataagaatcc gataaataag
     1261 aatactttgg attctccttt aaagatatac tgtaatgtga tcgcaattcc taatgcaggc
     1321 attaatccac cgataatctg gaatactgtt aagactttac cacttaacat cgctaagatt
     1381 ccttgaatat agctagatcc aaaatatgct gccaatgcac aagggatcgc cgtcatacag
     1441 aaacataaga tctgtggaag caatacgttt gcaatccaga ttttgttata tttttcttct
     1501 tcaatgtatt tatctgccat atgtacaaag aagcagtcaa atgtcatacg tcctgcccac
     1561 acaatcgttc ctaagatacc gatcggtact gcaagtgcta aggctgtatc tgtatcaagt
     1621 ccaccaacga tcgcatatgc tgtaccaagt acacctgcaa gtcccatatc tgccggcata
     1681 cttcctccag ctgacataaa tcctaaataa accaagttaa tcgttgctcc gatcacagca
     1741 ccctgtactg gatgtccaag gatcaaacct gtgatcgctc cacataccaa cggtctttgt
     1801 aaagaccaaa gtcctacgaa aggcaagtta ccaactgcaa gccagtaaac gaccccgcat
     1861 aacactgcct gtaaaatcgt catttccata ttattttcct cctttgaatc acattactca
     1921 tttaaatatc catcaacttc aattacctgc tgttctggta ctaactgata atacacatga
     1981 acacccttgg ctcttaaatt tttgatcgat gtaatttcat ctggagaaca cgctacattt
     2041 ttgataaatg gagttctttc tccacgaagt cccatacctc caagatttac atcactaagt
     2101 tttagtccca tatctgccat tctgtcgtaa gtgatcggtg tttttgtaag gatcataact
     2161 ctttcatttg gatctttgga atctctctgt aagatgtctg cccctttttc tgttccgtat
     2221 acattaacct ttactccgtt tggagctaat gctttgataa cacgtttatt gaatttatct
     2281 gctgcaactt catcatctac tacaatgatt ctgtttacat ttaaacttgg ggtccataca
     2341 gaaacaactt ctccatgaat caacctgtca tcaactcttg ctaatacaat gttcttcatt
     2401 cttcttcctc ctcatcatca aatagatcat ttacatattt tactgtgtct ggtgcagctt
     2461 ccataatccc tctgcagatt tcttcagcac tcttgtctgc atatcttccc atcataactt
     2521 caactgccag cggaatattg attcctgtaa tatgatggaa ttcatgatct ctcattaatg
     2581 aaacaactgc attaaatggg cttccatgga agagatctgt taagaaaaga atttcttcac
     2641 cttctggtgt atcattaatt tccttttcta acttttctgt taatgttgct actgtctgtt
     2701 ctggaaataa tccatatgct acaagacttt cctgttctcc taccagcatc tgcacactgt
     2761 ctacaagtcc tttggaatag cttccatgag atactgcgat tactttcatc acgtttcctc
     2821 ctttatatgt tgttttt
//
LOCUS       DAOMUE010000270         2827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10176_length_2827_cov_2.523088, whole genome shotgun sequence.
ACCESSION   DAOMUE010000270 DAOMUE010000000
VERSION     DAOMUE010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10176_length_2827_cov_2.523088"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLC71_09770"
     CDS             complement(<1..315)
                     /locus_tag="PLC71_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM02965.1"
                     /translation="MKKRKWNRVLSIFLVMVTVISLMSGCDRKSAKNEEKDTITVYLW
                     STNLYEKYAPYIQKQLPDINIEFVVGNNDLDFYRFLKENGGLPDIITCCRFSLHDANP
                     LKD"
     gene            complement(315..2495)
                     /locus_tag="PLC71_09775"
     CDS             complement(315..2495)
                     /locus_tag="PLC71_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRM02966.1"
                     /translation="MILDINGREKAKRFWIVASLLGICMFVTAIFCFFHTEKNEAEKR
                     MVEIVNYVKVQCSTYTHYNESSESKSLLRAIESARQMSTNIDMEVKNRKQLDRKFLKE
                     NLQSLWVDGILVLDEEGKKVCEYSMDEGLMDEMIDYLQKDIIMDYTGYKERSYSERIA
                     RGDGSRIDIAACARKDAPGIVAVYYYTSPRFIRNYTLTIQSLLKGYNTEKDGTIIVAD
                     KGKIIASNDEKLLAQDVADNEIIQKMKKHTDSRHIFHLKNKGTGCYGIMLKQRDYYIY
                     MYLPDKEVFSNLPLSVTGVVFLYLIILSFSWSWIYITNLAHQKQEQEKDEKYKAELLK
                     SAKKAEAANEAKTEFLQRMSHDIRTPINGICGMIDVAEHYADDMEKQTECRAKIKETS
                     HLLLELINEVLDMSKLESDEVVLEEIPFNLSNISKEIFVVIEQIAAEENIRIVWEKEE
                     ITHWNLIGSPGYVKRIMMNILSNAVKYNKENGYIYISYQEFTSEQEGRVTIEFICRDT
                     GIGMTKDFQKRLFEPFAQEHTGSRTKFSGTGLGMPITKKLIEKMGGTITFESKKGEGT
                     TFVIRIPFKIDQDADQREEQEAISEKSIKDLKILLVEDNELNMEIAEFVIQNEGASVT
                     KAWNGQEAVEIFKKSRQDEFDVILMDIMMPIKNGYEAAKMIRALDRDDAKTVPIIAMT
                     ANAFTEDRLKSKESGMNEHIAKPIDAKLLVKVISEFVENKEEDS"
     gene            complement(2479..>2827)
                     /locus_tag="PLC71_09780"
     CDS             complement(2479..>2827)
                     /locus_tag="PLC71_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRM02967.1"
                     /translation="KRTDKRIPKLGNLISLGHMELIYTFLGKGYVDSVAAHEESIVQY
                     MKDYDTSFRILKEPLMVVGIGAAFAKNDDRGICEQMNQKLEEMHKDGTSLKIIKKYMD
                     DPEKYLEVDNLGY"
BASE COUNT          719 a          675 c          421 g         1012 t
ORIGIN      
        1 gtctttcaga gggtttgcat catgtagtga aaaacgacag caagttatga tatccggcaa
       61 tccgccattt tccttaagaa accgatagaa atccagatca ttgttgccca ctacaaattc
      121 aatattaata tctggaagct gtttctgaat gtatggggca tatttttcat atagatttgt
      181 gctccataaa tatacagtga tcgtatcttt ctcttcattt ttcgcgctct tcctatcaca
      241 tcctgacata agtgatatga ctgtcaccat caccaaaaat atagaaagaa ctctattcca
      301 ttttctcttt ttcattatga atcctcctct ttattttcca cgaattcact gatcactttt
      361 actaataatt ttgcatctat aggtttcgcg atatgttcat tcattcccga ttcttttgac
      421 tttaatctat cctcggtgaa cgcatttgcc gtcattgcta tgatcggaac cgtttttgca
      481 tcatctctgt ccagtgcacg gatcatcttt gctgcttcat aaccattctt gatcggcatc
      541 atgatatcca tcagaatgac atcaaattca tcttgtctgc tttttttgaa tatctcaaca
      601 gcttcttggc cattccatgc cttcgtcaca gatgcacctt cattctgaat cacaaattct
      661 gcgatttcca tattcagctc attatcttcc accagaagga tcttcagatc ttttatagat
      721 ttttctgata tagcttcctg ttcttcacgt tgatcagcat cctgatctat tttaaatgga
      781 atcctgatca caaaggttgt gccttctccc ttcttacttt caaacgtgat cgttcctccc
      841 attttttcga tcagtttttt cgtgatcggc attccaagcc ctgttccgga aaattttgta
      901 cggcttccag tatgctcctg tgcaaacggt tcaaaaaggc gtttctggaa atcctttgtc
      961 ataccaattc ctgtatctcg gcagataaat tcaatcgtca ctcttccctc ctgctccgat
     1021 gtaaattcct ggtagctgat gtagatatat ccgttttctt tattatattt taccgcattt
     1081 gacaaaatat tcatcatgat cctttttaca tatcctggac tcccgatcag attccagtgt
     1141 gtgatttctt ctttctccca cacgatccga atattttctt ctgcagctat ctgttcgatc
     1201 acaacaaaga tttccttgga aatattgctt agattaaatg gaatttcttc cagaactact
     1261 tcgtccgatt ccagcttact catatccaaa acttcattga tcaattccag taacagatga
     1321 gatgtttctt tgatctttgc cctgcattct gtctgttttt ccatatcatc tgcataatgc
     1381 tccgccacat cgatcatacc gcatattcca ttgatcggtg tccggatatc atgactcatc
     1441 ctctggagaa actcagtttt cgcctcgttc gctgcctctg ctttttttgc cgatttcaga
     1501 agttctgctt tatatttctc atctttttcc tgttcctgtt tctgatgtgc cagattggtt
     1561 atatatatcc aggaccatga aaaactaaga atgataagat aaaggaatac aacacctgtt
     1621 acactgagtg gcagattact gaatacctct ttatctggta aatacatgta gatataataa
     1681 tccctttgtt tcagcatgat accatagcat ccagttcctt tattcttcag atgaaagata
     1741 tgccgactat ctgtatgctt cttcattttc tgaataatct cattgtctgc gacatcctgt
     1801 gccaataatt tctcatcgtt actggcaata atcttccctt tgtctgcaac gatgatcgtc
     1861 ccgtctttct cagtattgta cccctttaaa agactctgga tcgttaaagt ataatttctg
     1921 ataaatctcg gagatgtgta atagtaaact gcaacgatcc ctggggcatc ttttctagcg
     1981 caggctgcga tatcgatacg tgatccatct cctctggcaa tccgttctga ataagatctt
     2041 tctttatatc ctgtgtaatc catgatgatg tctttttgca gataatcaat catctcatcc
     2101 atcaaacctt catccatact gtattcacag actttctttc cttcttcatc caatacaaga
     2161 ataccatcca cccagagtga ctgcagattc tctttcagaa actttcggtc taactgcttt
     2221 ctattcttta cttccatatc aatatttgta ctcatctgtc tggcactttc gatcgcacga
     2281 aggagacttt ttgattctga agattcatta taatgagtat aggttgagca ctgtactttg
     2341 acatagttta caatctccac cattcttttc tcagcttcgt ttttttccgt atgaaaaaaa
     2401 caaaaaattg cagtgacaaa catacagatg ccaagcaaac tagcaactat ccagaatcgt
     2461 tttgcttttt ctcttccatt aatatccaag attatccacc tccaaatatt tctctggatc
     2521 atccatatat ttttttatga tcttcaggga ggtaccatcc ttgtgcattt cttccagctt
     2581 ctgattcatc tgttcgcaga tccctctgtc atcattcttt gcaaaagcag ctcctatccc
     2641 gacgaccatc aatggttcct tcagaatgcg gaagcttgta tcataatctt tcatatactg
     2701 gacgatggat tcctcatgcg cagccacaga atccacatac cctttcccca gaaatgtata
     2761 gatcaattcc atgtgtccaa gactgatcag attgccaagt ttgggaattc tcttatccgt
     2821 ccgcttc
//
LOCUS       DAOMUE010000271         2824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10193_length_2824_cov_2.988082, whole genome shotgun sequence.
ACCESSION   DAOMUE010000271 DAOMUE010000000
VERSION     DAOMUE010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10193_length_2824_cov_2.988082"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..612
                     /locus_tag="PLC71_09785"
     CDS             133..612
                     /locus_tag="PLC71_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HRM02968.1"
                     /translation="MKRKDYISWDQYFMGIAILSAQRSKDDHTQVGACLVNDHNKILS
                     VGYNGMPIGCNDDDMPWEREGDPLHTKYFYVCHAELNAILNYGGGSLDGARVYVTLFP
                     CNECAKAIIQSGMKEVIYMQDKYAGSDTTKASKKMFDMAGVKYRQFVPKGQTIEVDL"
     gene            622..2763
                     /locus_tag="PLC71_09790"
     CDS             622..2763
                     /locus_tag="PLC71_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRM02969.1"
                     /translation="MDIIKILQEELGIRRGQVETTIKLIDEGNTIPFIARYRKEMTGS
                     LDDETLRNFHERLLYLRNLEERKEAIKKNIEEQGKLTKELAKQIEEAKTLVAVEDLYR
                     PYKQKKRTRAMIAKEKGLEPLANLILLQMTKEPLEKEAEAFINEEKDVKTVEDALKGA
                     NDIIAEHIADDAEYRTWIRKATWDHGKITSSAKKPEESSVFEMYYDFEEPIEKIAGYR
                     ILAINRGEKEGILQVKIEPDMQKIASYLARKIITRKNPNTTKALFAAIEDSYKRLIAP
                     SIERDIRNELTENASTEAIKVFGKNLAQLLMQPPIAGQVVLGWDPAFRTGCKLAVVDA
                     TGKVLDTVVIYPTAPQNKVEEAKKVVKALIKKYGITLISIGNGTASRESEQIVVDMLK
                     EIKEPVQYVITNEAGASVYSASKLATEEFPNFDVGQRSATSIARRVQDPLAELVKIEP
                     KAIGVGQYQHDMNQKQLTEALGGVVEATVNKVGVDLNTASASLLEYVSGVSKTVAKNI
                     VAYREENGTFRNRKQLLKVAKLGPKAFEQCAGFLRVSGGDEPLDATGVHPETYKETGM
                     LLKNLGYSTKELSKEGFKGISSKITDYKKLSEELGIGEITLKDIVKELEKPGRDPRED
                     LPKPILRTDVLEMKDLKPGMKLKGTVRNVIDFGAFVDIGVHQDGLVHISQMADRFIKH
                     PLEVVSVGDVVDVVVVSVDIDKKRIQLSMKL"
BASE COUNT         1067 a          463 c          626 g          668 t
ORIGIN      
        1 tttttcttat aaatatttct tcttatcttc tttctgactc atacatgcaa cactacctac
       61 aaatcaaaag ttgacgatat cgtagaaaca tgacaaaata tatctacaaa gattcagaaa
      121 ggaatatgta caatgaaacg taaagattat atctcctggg atcagtattt tatggggatt
      181 gccattttat ctgcccagag aagtaaagat gatcatacac aggtaggggc ttgccttgta
      241 aatgatcata ataagatttt gtcagttggt tataacggaa tgccaatagg atgcaacgat
      301 gatgatatgc cttgggagag agaaggagat cctcttcata ccaaatattt ttatgtatgt
      361 cacgcagaat taaatgccat tttgaactat ggtggaggct cacttgacgg agcgcgtgtt
      421 tatgtaacat tatttccgtg caatgaatgt gcaaaagcta tcattcaaag cggtatgaaa
      481 gaagttatct atatgcagga taaatatgca ggcagtgata cgacaaaggc atcaaagaag
      541 atgtttgata tggcaggagt taaatatcgc cagttcgtac caaaaggaca gacaatcgaa
      601 gtagatttat aaggtgaaga tatggatatt attaagattt tacaagaaga actaggaatc
      661 agacgaggac aggtagagac aacgatcaaa ctgatcgatg aaggtaacac gattccattc
      721 attgccagat accgaaaaga aatgacagga tcattagatg acgagacact tcgtaatttc
      781 catgagagat tattatattt aagaaattta gaagagcgca aagaagcgat caagaaaaat
      841 atcgaagaac aaggtaaact tacaaaggaa ctggcaaagc agattgaaga agcgaagaca
      901 ctggtagcag tggaagattt gtaccgtcca tataagcaga aaaaacgtac cagagctatg
      961 atcgcaaagg aaaaggggtt agagccgtta gctaatctga ttcttttaca aatgacaaaa
     1021 gagccattag agaaagaagc cgaagcgttt atcaatgaag aaaaagatgt aaagacagta
     1081 gaagatgcat taaaaggtgc aaatgatatt atcgcagagc acattgcaga tgatgcggaa
     1141 taccgtacat ggatccgtaa ggcaacatgg gatcacggaa aaatcacatc ctcagcgaag
     1201 aaaccagaag aatcttctgt atttgagatg tattatgatt ttgaagaacc gattgaaaag
     1261 attgcaggat accgtattct tgcgatcaat cgcggagaaa aagagggaat tcttcaggta
     1321 aagattgagc cagacatgca aaagatcgca tcatatcttg caaggaagat cataacaagg
     1381 aagaatccca atacaacaaa agccttattt gcagcgattg aagacagcta taaacgtctg
     1441 atcgcaccat cgatcgaacg tgatatcaga aatgaactga ctgaaaatgc aagtacagaa
     1501 gcgatcaaag tctttggaaa gaacttagca caacttctta tgcagccacc gatcgcagga
     1561 caggtcgtcc ttggatggga ccctgcattt cgtactggat gcaaattagc agtggtcgat
     1621 gcaacaggaa aagtattaga tacagtcgtc atttatccga cagcaccaca gaataaagtg
     1681 gaagaggcca aaaaagttgt caaggcactc atcaagaaat atggaatcac actgatctca
     1741 attggaaatg gaacagcatc cagagaatct gaacagatcg ttgttgatat gttaaaagag
     1801 atcaaagaac cagtacagta tgtgattaca aatgaagcag gggcgtctgt atactcagca
     1861 agtaaacttg cgaccgagga atttccaaat ttcgatgtag gacaaagaag tgcgacatcc
     1921 atcgcaagac gagtacaaga tccactggca gaattagtaa agatcgaacc aaaagccatt
     1981 ggagtcggac agtatcagca cgatatgaac caaaaacagc ttaccgaagc tttaggtgga
     2041 gtcgttgaag caacagtaaa taaagtcgga gttgacctaa atacagcttc agcatctctt
     2101 ttagaatatg tatccggtgt gtccaagact gtagcaaaaa atatcgtagc ctaccgtgaa
     2161 gaaaatggaa cgttcaggaa cagaaaacag ctattaaaag tagctaaact aggacccaaa
     2221 gcctttgaac aatgcgcagg attcctaaga gtatctggag gtgatgagcc actcgatgca
     2281 acaggagtac atccagagac atacaaagaa acaggaatgt tactaaagaa cctaggatat
     2341 tcaacaaaag aactgtccaa agaaggattc aaaggaatca gcagtaagat cacagattac
     2401 aagaaattat ccgaagaact tggaatcgga gagatcacgt tgaaagatat cgtaaaagaa
     2461 ctggaaaagc caggacgaga tccacgagaa gatcttccaa aaccaatcct aagaacagat
     2521 gtattagaaa tgaaagatct gaaaccagga atgaaattaa aaggaacagt tcgtaatgtc
     2581 atcgattttg gagcatttgt agatattgga gtacatcagg atggactagt acacatttca
     2641 cagatggcag atcgatttat caaacatcca ttagaagtag ttagtgttgg agatgttgtg
     2701 gacgttgtag ttgtcagtgt ggatatagat aaaaaacgga tacagttaag catgaaatta
     2761 tgatttgtat gtaggcttgc atgtatgtga cagaaataaa agaaaaacaa atatttgaaa
     2821 gaat
//
LOCUS       DAOMUE010000272         2802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10287_length_2802_cov_2.900983, whole genome shotgun sequence.
ACCESSION   DAOMUE010000272 DAOMUE010000000
VERSION     DAOMUE010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10287_length_2802_cov_2.900983"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1483
                     /locus_tag="PLC71_09795"
     CDS             155..1483
                     /locus_tag="PLC71_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRM02970.1"
                     /translation="MSINTADFAALAVTIVLFAVIYYLDYKKGVDFGLLTLLGTVFGI
                     AVGLGFGNHFTYVEIFGRIYTNVLTALVVPMLLFSIIASITNLSESIHLKKIGGKSIF
                     YLILNTAIASTITVIAAVILKVGDGFAYKVADGYQKVEVPSVVDTIVNLFPSNFATNW
                     SEGQIIPIVVFAIIIALSYNAISKENESVKPFKAFIDAGNQVIGQAIDWIIGFTPYAV
                     LSFLARAVGRSSVTELLPLLSTLVLSYILCAVQAFGVEGLLIKFVAKLSPVKFFKGIA
                     PAQYVAFTTQSSVGTIPVTLRQLKEALGVDGDVASFTAGLGANVGMPACAGIWPALTA
                     VFSVHMLGYHYGIAQYAFLILITLVVSVGTVGVPGTATITSTAVFAALGLPIELVVVF
                     APISTIVDMARTMTNVTGAATAAVITAATEGLVDEEVYNSDQAKHVAVNN"
     gene            1689..>2802
                     /locus_tag="PLC71_09800"
     CDS             1689..>2802
                     /locus_tag="PLC71_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009264208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM02971.1"
                     /translation="MGNYLNPGNEKFRRMIQSEIYVDKTGMIKYTNSVLDTAQNYVCV
                     SRPRRFGKSMAANMLTAYYSRGCNSKELFGKFEIAKDKDFEKYLNQYNTIFLNMQEFL
                     SRSHNVEELIDRVKKLVIRDLKMEYPEVDYFDDTDLIECMQDIYAQMQYPFVVIIDEW
                     DCIFREYKQDKEAQEKYLDFLRDLLKDKACIYLSYMTGILPIKKYGTHSALNMFDEFS
                     MIAPGPLACYVGFTEQEVKKLCEQYGMDMEEVKNWYDGYSFDGVLSVYSPRSVVNSMR
                     FQKISNYWNQTETFEALQMYIDMNFEHLKDDVLSMIAGESVAVNTESFTNDMATFRTE
                     DDVLTLLIHLGYLAYDDKTKTVKIPNSEIRAEYVNTV"
BASE COUNT          966 a          408 c          580 g          848 t
ORIGIN      
        1 attatgcttc ataaacattc caacaaatca aaattgtata aaaaaagaag catcaaatat
       61 tcttgacagt attactagga aatgatatta tacgaataat tcctactgga aaactatgaa
      121 aaagaaaaaa ctataagaga ggatgtgtaa caaaatgagc attaacacag cagattttgc
      181 cgcactagca gttacaattg ttttgtttgc ggttatctat tatttagatt ataagaaagg
      241 cgtagacttc ggacttttaa cattattagg aacagttttt ggaatcgcag ttggtctggg
      301 atttggaaat cattttacat atgttgagat tttcggacgt atctatacca atgtattgac
      361 agcacttgtt gtaccaatgc tgttatttag tatcatcgca agtatcacaa atctaagtga
      421 atccattcat ttaaagaaaa tcggtggaaa gtcaatcttt tatctgatct taaatacagc
      481 gatcgcaagt acgattacag tgatcgcagc agtcatctta aaagttggtg atggatttgc
      541 ttacaaagta gctgatggat atcagaaagt tgaagttcca tctgttgttg atacgatcgt
      601 aaacttattc ccaagcaact tcgcaacaaa ctggagtgaa ggacagatca ttccgatcgt
      661 cgtatttgcg atcatcattg cattatctta caatgcaatt tccaaagaaa atgaatccgt
      721 aaaaccattt aaagcattta ttgatgcagg aaatcaggtg atcggacagg caattgactg
      781 gatcattgga tttacaccat atgcagtatt atctttcttg gcaagagctg ttggaagaag
      841 cagtgtcacg gaattgctgc cattattaag tacattagta ttatcttata tcttatgtgc
      901 agttcaggca tttggagtag aaggattatt aattaaattt gtagcaaaat taagtcctgt
      961 taaattcttt aaaggaatcg caccagctca gtatgtagca tttacgacac aaagcagtgt
     1021 tggtacgatc ccggttacat taagacagtt aaaagaagca cttggagttg atggagatgt
     1081 agcatcattt acggcaggtc ttggagccaa tgttggaatg ccagcatgtg caggaatctg
     1141 gccagcatta acagcggtat ttagtgttca tatgttagga tatcattacg gaattgcaca
     1201 gtatgcattt ctgatcctga tcacattagt agtatctgtt ggaacagtcg gggttccagg
     1261 aacagcgacg atcacatcaa cagctgtatt tgcagcactt ggcttaccaa tcgaattagt
     1321 tgtcgtattt gcaccaatct caacgatcgt tgacatggca agaacaatga caaatgtaac
     1381 aggagcagca acagcagccg ttatcacagc agcaactgaa ggtctggttg atgaagaagt
     1441 ttacaattca gatcaggcaa aacatgttgc agtaaacaac taggatagaa gaaattctat
     1501 atataatcac tgtatgaaaa gacacttgat cgtaacgtat attttatgta tgttatgagc
     1561 aggtgttttt tcatttcatg taattaatat aagttgaggc ggctaaaatt tcgtgctatc
     1621 ataatcaata tagataagag cacgaagttt tttcatatta tagaaaattg attcaaggag
     1681 gtgatcgtat gggaaattat ctaaatccag ggaatgaaaa gttcagaaga atgattcagt
     1741 ctgaaattta tgtagataag acagggatga taaaatatac gaattccgta ctagatacag
     1801 cacaaaacta tgtctgtgtc agccgtccaa gaagatttgg gaagtcaatg gctgcgaata
     1861 tgctgacagc ttattacagc cgcggttgta attcaaaaga attatttgga aagtttgaaa
     1921 ttgcaaaaga taaagatttt gaaaaatatc tgaatcaata taataccata ttcttaaata
     1981 tgcaggaatt tctaagtcga agtcataatg tagaagaatt gatcgatcgt gtgaagaagc
     2041 ttgtgatcag agatttgaaa atggaatatc cagaagttga ttattttgac gatacagatt
     2101 tgattgagtg tatgcaggat atttatgcac aaatgcagta cccatttgtt gtgatcattg
     2161 atgaatggga ttgcattttt agggaatata aacaagataa agaagcacag gaaaaatatt
     2221 tagattttct acgggactta ttaaaagata aggcatgtat ttatctatct tacatgacag
     2281 gaattttacc gattaagaaa tatggaacac attcggcact gaatatgttt gatgaatttt
     2341 ccatgatcgc cccgggacct ttagcatgtt atgtcgggtt tacagaacaa gaagtcaaaa
     2401 agctctgtga acaatacgga atggatatgg aagaagttaa gaactggtat gatggatatt
     2461 catttgacgg agtactgtct gtgtatagtc caagatcagt tgtaaatagt atgagatttc
     2521 aaaagatatc aaattactgg aatcagacag agacttttga agcattacag atgtatattg
     2581 atatgaattt tgagcatcta aaagatgatg ttttaagtat gatcgctgga gaaagcgtgg
     2641 cggtgaatac cgaaagtttt acgaatgata tggcaacgtt tcgtacagaa gatgatgtac
     2701 tgacattatt gatccatctt ggatatcttg catatgatga taaaacaaaa acagtcaaga
     2761 taccaaatag tgagattcgg gcagaatatg taaatacagt tt
//
LOCUS       DAOMUE010000273         2799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10308_length_2799_cov_2.258382, whole genome shotgun sequence.
ACCESSION   DAOMUE010000273 DAOMUE010000000
VERSION     DAOMUE010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10308_length_2799_cov_2.258382"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /gene="rlmB"
                     /locus_tag="PLC71_09805"
     CDS             <1..610
                     /gene="rlmB"
                     /locus_tag="PLC71_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRM02972.1"
                     /translation="AKKTDAILKFVDKERLNQLTNEHHQGVVAIVAAYEYGTIEDLFK
                     RAEEKGEDPFFILLDGIEDPHNLGAIIRTANLAGAHGVIIPKHRAVGITPTVAKTSAG
                     AINYTPVVKVTNIGKTMDELKERGMWFACADMDGEVIYRQNLTGSIGLVIGNEGSGVS
                     RLVKEKCDFISSIPMKGDIDSLNASVAAGVLAFEVVRQRLGK"
     gene            614..1216
                     /locus_tag="PLC71_09810"
     CDS             614..1216
                     /locus_tag="PLC71_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRM02973.1"
                     /translation="MKELKTISDEKLLRQIQSGNDDAMECLLERYRDMVRKEARSFFL
                     AGGDEEDLIQEGMIGLFKAVTSYQEEKNTSFSTFAYLCVQRQIYTTITAFNRKKHIPL
                     NTAISLFEQKNQEEELSLDEILETPEETPEEMMLRKEELNDYYKMLDQNLSKFEKQVM
                     YHYLNGETYTTIAKKLGKSDKSIDNAIQRIRRKIRNDQES"
     gene            complement(1277..1349)
                     /locus_tag="PLC71_09815"
     tRNA            complement(1277..1349)
                     /locus_tag="PLC71_09815"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1314..1316),aa:Thr,seq:ggt)
     gene            1421..2734
                     /locus_tag="PLC71_09820"
     CDS             1421..2734
                     /locus_tag="PLC71_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02974.1"
                     /translation="MTKQYRKNKRRICVVICLVLCMISGIKIQAAQPGYFDNPQTVYD
                     EFAGKIYDRSFKKSYAFLKKKMNVFETSSGSKKIGVAPRYSGVIVVSKTSDHVQVIYE
                     KKKAYGIGWIDRGLYHKEAIPYNGNEKQLLGNGTYWIQNKASGEGIRVQIIFLGNQKY
                     QVSILNQSKEDHTWKLIREYDHFYIKNMKNEKYLSMDENGTLTDGKIKDMNNCFTKPQ
                     KEAAQKSYQWQVMRLNNKNVYPYRDFMQFDPAWARKDYGNVSDYSGKMAAAGCGVVAI
                     TNAVYALNGQFVDPMLFADYAVEKHYRIIGAGTHDGIFKAAAKKFGDTYGFTYIKTTY
                     STSEVREYLKKGCVAISHVPGHYVTVADFNPKTKKYLVLDSHPIKSRPTSSFGNWFKR
                     ERLERGGLTSSAYYIYGVPGQAWKYESAKGIQFQKDLFTFMIYMR"
BASE COUNT         1042 a          392 c          635 g          730 t
ORIGIN      
        1 ggcaaagaaa acagatgcga ttcttaaatt tgtagataaa gaacgcttga atcagctgac
       61 aaatgaacat catcagggag ttgttgcaat tgtcgcagca tatgaatatg gaacgattga
      121 ggatctgttt aaaagagcag aagaaaaggg tgaggatcct ttctttatcc tgttagatgg
      181 gattgaagac cctcataatc ttggggcaat cattcgtaca gcaaatcttg caggtgcaca
      241 tggagtgatc attccaaagc atcgtgcagt tggaattaca ccaaccgtag caaaaacatc
      301 tgcaggtgcg atcaattata caccagttgt gaaagtaaca aatattggaa aaacaatgga
      361 tgaattaaaa gaacgtggta tgtggtttgc atgtgcagat atggatggag aagtgattta
      421 tcgtcagaat ctgacaggat cgatcgggct tgttattgga aatgaaggaa gcggtgtgag
      481 ccgtttggta aaggaaaagt gtgactttat ctcatctatt ccaatgaaag gagatattga
      541 ttcactgaat gcttctgtag cagcaggtgt tctggcgttt gaagttgtaa gacaacgact
      601 gggaaaataa agaatgaaag aattaaaaac aatttctgat gagaaattgc tcagacagat
      661 ccaaagcggt aatgatgatg cgatggaatg tctgttagag agatacaggg atatggtaag
      721 gaaagaggca aggagctttt ttcttgcagg tggggatgaa gaagatctga tccaggaagg
      781 aatgatcggg ctttttaaag cagtgacatc ctatcaggaa gaaaaaaaca cttcgttttc
      841 aacgtttgct tatctgtgtg tgcaaagaca gatctataca acgatcactg catttaacag
      901 aaaaaaacat attccgttga atacagcaat ctctctgttt gaacaaaaga atcaggaaga
      961 agaattatca ttggatgaaa tcttagaaac tccggaggaa actccggagg agatgatgct
     1021 tcgaaaggaa gaattaaatg attattacaa aatgcttgac cagaatttaa gtaaatttga
     1081 aaaacaggtg atgtatcatt atctgaatgg agaaacatat acaacgattg caaaaaaact
     1141 tggaaaaagt gataaatcca ttgataatgc gattcagagg attcgcagga agatcagaaa
     1201 tgatcaggaa agttaaagta aagataagag aaataaaaag aagacttaaa acagatgttt
     1261 taagtcttct ttataaagct gctgacggga gttgaacccg tgacctcctc actaccaatg
     1321 aggtgcgcta cctcctgtgc cacagcagca gaactgatta ataatttatc atgaaaatga
     1381 tatactgtca atagaattaa caaaataaaa ggaaaatcaa atgacaaaac aatacaggaa
     1441 aaataaaaga agaatctgtg tagtgatatg ccttgttctt tgcatgatct caggcatcaa
     1501 aatacaggca gcacagccag gatattttga taatccccag acggtctatg atgaatttgc
     1561 tgggaagatt tatgatcgtt cattcaaaaa gagttatgca tttttgaaaa aaaagatgaa
     1621 tgtttttgaa acatcttcag gaagtaaaaa gataggggta gcgccaagat attcaggagt
     1681 gatcgtggtt agtaaaacat ccgatcatgt tcaggtgatc tatgagaaaa agaaagcata
     1741 tggaattgga tggattgata gaggtttgta tcataaggaa gctattccat ataatgggaa
     1801 tgaaaaacag ttgctaggaa atggaactta ttggatacaa aataaagctt ctggagaagg
     1861 aattcgtgta cagatcattt ttctgggaaa tcagaaatat caagtttcga ttttgaatca
     1921 aagcaaagaa gatcacacat ggaaactaat acgtgagtac gatcattttt atatcaaaaa
     1981 tatgaaaaat gaaaaatatc tgtcaatgga tgaaaatggt accttgacag acggtaaaat
     2041 aaaagatatg aacaactgtt tcacaaaacc acagaaagaa gcagcacaga agtcctatca
     2101 atggcaggta atgcgtctta acaataaaaa tgtatatccg tatcgtgatt ttatgcagtt
     2161 tgatcctgca tgggccagaa aagattatgg aaatgtttcg gattacagtg ggaaaatggc
     2221 agcagcaggc tgcggagttg tggcgatcac aaatgcagta tatgcattga atggacaatt
     2281 tgttgatcca atgttgtttg cagattatgc agtggagaaa cattacagga tcataggtgc
     2341 cggaacacat gatgggattt ttaaggcggc agcgaagaaa tttggagaca catatgggtt
     2401 cacttatatt aagacaactt acagtacatc agaagtaaga gaatatctga aaaaaggttg
     2461 tgtagcaatc tcgcatgtac caggacatta tgtaacagtt gctgatttta atcccaagac
     2521 aaaaaaatat ctggtcttag attctcatcc gatcaagagc agaccaacca gctcttttgg
     2581 aaactggttt aagagagaac gattagagag aggagggttg acatcatctg catattatat
     2641 ttatggagtg ccaggacagg catggaaata tgaaagtgca aaaggaatac aatttcagaa
     2701 ggatttattc acatttatga tttacatgcg ttaaaatgta agaaaaaata acaaaggata
     2761 gggaaggaga agttaaatga acaaagttaa aaagatatt
//
LOCUS       DAOMUE010000274         2794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10332_length_2794_cov_2.228916, whole genome shotgun sequence.
ACCESSION   DAOMUE010000274 DAOMUE010000000
VERSION     DAOMUE010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10332_length_2794_cov_2.228916"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLC71_09825"
     CDS             <1..625
                     /locus_tag="PLC71_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRM02975.1"
                     /translation="KLLIENGLQATLTLFFLTLIFGLILGMIIAKGRMSKLLIIQMPI
                     RFFLLIMRGTPLILQLYGFYFGLYYIAGLSLDRMTAAVIGFSLNYAAYFAEIYRSGIQ
                     AIPKGQYEAAKVLGFNRSQTFFKIILPQVVKIITPSLGSECMTLVKDTSLAHVIGVME
                     IYVVATNQMARSRGMIYLVVAGIFYLIMNAIVSKVFSVIEKRMNYYR"
     gene            638..1399
                     /locus_tag="PLC71_09830"
     CDS             638..1399
                     /locus_tag="PLC71_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRM02976.1"
                     /translation="MSILSVKNLEKKFGDLTVLKDISFNINDGEIISIIGSSGSGKST
                     LLRCMNQLETITSGTITIDGKTLVETKNGAPVYAGKDILKEILMETGFVFQNFNLFPH
                     YSVLRNVMEAPVCVAGVPKKQAEEKARELLKKLGLETKADAYPCELSGGQSQRVSIAR
                     ALALEPKILFFDEPTSALDPELTGEVLKVIKSLTDLNITMVIVTHEMAFAKEISDRII
                     FMDKGVIIEDASPDEVFSSDNERTREFLGKYHDVN"
     gene            1434..>2794
                     /locus_tag="PLC71_09835"
     CDS             1434..>2794
                     /locus_tag="PLC71_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRM02977.1"
                     /translation="MVKDMPNKFSEVTPEIKELAKKCNKKIDEELFAKYDVKRGLRKR
                     NGEGVLAGLTDISMINAYTMIDREIVPCEGKLYYRGIDIEDIVKGFIEEDRFGFEETA
                     YLLLFGELPKKDQLKQFEGMLGEYRQLPTNFVRDIIMKAPSCDMMNTLARSVLTLFSY
                     DDNASDISLPNVLRQCIQLIALFPVLSVYAYQAYNHYERGESLFIHLPDPELSTAENI
                     LHLLRPDSKYTKLEAKLLDMALVLHAEHGGGNNSTFTTHVVSSSGTDTYSAIAAALCS
                     LKGPKHGGANIKVVQMFDDLKANVKDWSNEEEIREYLLKLLNKEAFDKAGLIYGMGHA
                     VYSLSDPRSKILSRFVKQLSEEKGKEEEYQLYATVERLAKQVIGEKRKIYKGVSANID
                     FYSGFIYSMLGLPHQLYTPLFAIARIVGWSAHRMEELMNGNRIIRPAYKAVAPHREYT
                     PID"
BASE COUNT          951 a          466 c          592 g          785 t
ORIGIN      
        1 aaagttatta attgaaaatg gtctgcaagc aacattaaca ttattctttc tgactttgat
       61 ttttggattg attcttggta tgataattgc taaaggaaga atgtcaaagc ttctgatcat
      121 tcagatgcca attcgatttt tcctattgat catgagagga acaccactga tcctgcagtt
      181 atatggattc tattttggac tatattatat cgcaggatta agccttgatc gtatgacagc
      241 ggcggttatc ggtttttctt taaattacgc agcatatttt gctgagattt atcgaagtgg
      301 tattcaggca attccaaaag gtcagtatga agcagcgaaa gtattaggat ttaacagatc
      361 acagacattc tttaagatca ttttacctca ggttgtaaag atcatcacac catcccttgg
      421 aagtgagtgt atgacattgg ttaaggatac atccttagct catgttattg gtgttatgga
      481 gatttacgtt gtggcaacaa accagatggc acgaagccgt ggaatgatct atcttgttgt
      541 agcaggtatt ttctacttga tcatgaatgc cattgtatca aaagtattct cagtcattga
      601 gaaaagaatg aactattatc gttagggagg aaagaagatg agtattttat ccgttaaaaa
      661 cttagagaag aaattcggag atcttaccgt attgaaagac atttccttca acatcaatga
      721 cggcgagatc atttcgatca ttggatcatc aggatcagga aaatcaacat tactgcgttg
      781 tatgaatcag ttagagacga tcacatccgg aacgatcacg atcgatggaa agacactggt
      841 tgagacaaag aatggagcac cagtctacgc aggcaaagat attttgaaag aaattttaat
      901 ggaaacagga tttgtatttc agaactttaa cctgtttcca cactatagtg ttctaagaaa
      961 tgtaatggaa gcccctgtat gtgttgcagg agtacctaag aaacaggcag aagagaaggc
     1021 aagggagctg ttaaagaaat taggtcttga gacaaaggca gatgcttatc catgtgaatt
     1081 atccggtgga cagtcacagc gagtatcgat cgcaagagca ttagcattgg aaccaaagat
     1141 cttgttcttt gatgaaccga cttctgcact tgatccagag cttactggag aagtattaaa
     1201 agtcatcaaa tcactgacag atctaaatat cacgatggtg atcgtcacac acgagatggc
     1261 ttttgcaaaa gaaatttcag acaggatcat ttttatggat aaaggagtca tcattgaaga
     1321 tgcatctcca gatgaggtat tcagttctga taatgaaaga acaagagaat tccttggaaa
     1381 ataccatgat gtaaactaga aatgatagca attgttagaa gggagaaaaa acgatggtta
     1441 aagatatgcc gaataagttt tcagaggtaa cccctgagat caaagaactg gctaagaaat
     1501 gtaataagaa gattgatgaa gagttgtttg caaaatatga tgtaaaaaga ggtcttcgta
     1561 aacgtaatgg tgaaggtgtc cttgcaggat taactgacat ttccatgatc aatgcttata
     1621 caatgatcga ccgtgaaatc gtgccatgtg aaggtaaatt atattaccgc ggaattgata
     1681 tcgaggatat cgtaaaggga tttatcgaag aagatcgttt tggatttgaa gaaacagcat
     1741 acttactgtt gtttggagaa ctccctaaaa aggaccagtt aaaacagttt gaaggaatgc
     1801 ttggagaata cagacagctt cctacaaact ttgtaagaga tatcatcatg aaagcaccaa
     1861 gctgtgacat gatgaataca ttagcacgaa gcgtcttaac attattttca tatgatgaca
     1921 atgcaagtga tatttcactg ccaaatgtac tgcgtcagtg cattcagctg atcgcattat
     1981 tccctgtact ttctgtatat gcatatcagg catacaatca ttatgagaga ggagaaagtt
     2041 tattcattca tcttccagat ccagaacttt ctacagcaga gaatatcctt catctgttaa
     2101 gaccagacag caaatataca aaactggaag caaaattact agatatggcg ttagtgcttc
     2161 atgcagagca tggtggaggt aacaactcta cgttcacaac acacgttgta tcatcttcag
     2221 gaacagatac atattcagca attgcagcag ccctttgctc attaaaagga ccaaaacatg
     2281 gtggagcaaa cattaaagtc gtacagatgt tcgatgatct aaaagccaat gtcaaagact
     2341 ggtccaatga agaagaaatt cgtgaatatt tattaaaact gctgaataaa gaagcattcg
     2401 acaaagcagg attgatctat ggtatgggac atgcagtata ttcattatca gatccacgtt
     2461 ccaagattct ttcacgcttc gtaaaacagc tttctgaaga aaaaggaaaa gaagaagaat
     2521 atcaattgta tgcaacggta gaacgtcttg ccaaacaggt cattggtgag aagagaaaga
     2581 tctacaaagg agtcagcgca aacatcgact tctacagtgg atttatctac agcatgttag
     2641 gattaccaca tcagttatat acaccattat ttgcgatcgc acgtatcgta ggatggtcag
     2701 cacatcgaat ggaagagttg atgaacggta atcgtattat tcgtccggct tataaagctg
     2761 tggcaccaca tagagaatat acaccaattg atga
//
LOCUS       DAOMUE010000275         2789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10360_length_2789_cov_2.272860, whole genome shotgun sequence.
ACCESSION   DAOMUE010000275 DAOMUE010000000
VERSION     DAOMUE010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10360_length_2789_cov_2.272860"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PLC71_09840"
     CDS             <1..683
                     /locus_tag="PLC71_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HRM02978.1"
                     /translation="FFDSTLKYQLSEDELPSEEENPDLPHVAIAVLPIIVILVLFNNP
                     WHPFPVETSVFAGVALATILMFKRIDGMTEWINIFNKGAADSGVSILNTAIVVGFGGV
                     VAKTQGFTDLVAGLKHLSMPPLIFVMVTVAVCAGACGSASGGMGVAFKALKDTYLKLG
                     ASLPDIHRISTIAAGTLDTLPHQGAQITLLGICKLTHKEAYWDIAVTQIFIPLIDCLV
                     FIALTSIF"
     gene            complement(756..1766)
                     /locus_tag="PLC71_09845"
     CDS             complement(756..1766)
                     /locus_tag="PLC71_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRM02979.1"
                     /translation="MNLGKKEGIVVVGEIQKREIMPLTLELIGAAGKLSKERDLSLSV
                     ILSGCGIKEEAKKLYNYGVDEILCIEDEKLIEGHMQLHHDAVIEVLKEKDPKVILIGG
                     TASGRVLAGKTAYAFDTELVCDASKLTYDEKQEQLIITRPAFDGKNMSDFLINPSLTS
                     VITIRTGVMGKAEYKEDKQGEMLYVHPECLKEERQDLIYHGAAGKSGKEVHLDTARII
                     VSGGRGMKGEEGFALLERLAEKIGGEVACTRPCVDAGWMLPTQQVGQSGISVKPKLYL
                     AFGIAGAIQHMTGIDADCIISVNNNPRAAIFAYSDYGVVSDAKKLLESMLKQMEEGKE
                     FL"
     gene            complement(1778..2536)
                     /locus_tag="PLC71_09850"
     CDS             complement(1778..2536)
                     /locus_tag="PLC71_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HRM02980.1"
                     /translation="MRFVVILKQIPDDSIKDEYQDVDQLNDSDKNVIKEALDLRDRYG
                     GTVDVIGFGPLSAEDVMKETLTYGIDDAFLISDSQFADMDVSQVAKIVAAVIKKLGPY
                     DLVLCGRQAIDGDSAHMASMTSCALEIPLIAYSDEITEMKDGSVFATCMGDQMAYKVE
                     AKTPAMILSIREKNKNRFPSVPDIMKTYDGTYKTKILTNEDLNFSVTKRKVTQLRKYE
                     VKSSKQQKLVMIGGKDEEEKAAQILQLLKQKSVI"
BASE COUNT          699 a          650 c          441 g          999 t
ORIGIN      
        1 gattttttga cagtacttta aaatatcaat tatctgagga tgaactgcct tctgaagaag
       61 aaaacccgga tcttccacac gttgccatcg cagtccttcc gatcatcgtg atcttagtat
      121 tatttaataa tccatggcat cctttccctg ttgaaacttc tgtatttgca ggtgttgcac
      181 ttgccacgat cttaatgttt aaacgaatcg acggcatgac ggaatggatc aatatcttta
      241 ataaaggtgc cgctgattct ggagtttcta tccttaacac agctatcgtt gtaggatttg
      301 gtggtgttgt tgcaaaaaca cagggattta ctgatcttgt tgcaggatta aaacatctat
      361 ccatgcctcc tttaatcttc gttatggtaa ccgttgctgt ttgtgctggt gcctgcggat
      421 ctgcttctgg tggtatggga gtcgctttta aagcattaaa agatacttat ttaaaacttg
      481 gtgccagcct tcctgatatc catagaattt ccacgatcgc cgctggaact ttagataccc
      541 ttcctcatca gggagctcag atcactttat taggtatctg taaattaaca cataaagaag
      601 catactggga tatcgcagtc acacagatct tcattcctct gatcgactgt cttgtattca
      661 ttgcgttaac cagtattttc taagacttac ctttatatta aaaaaagtat gctgtatatc
      721 aaaaccagat acacagcata cttttttatc catttttata agaattcttt tccttcttcc
      781 atctgtttta acatactctc cagtaatttc tttgcatccg aaaccacacc ataatcactg
      841 tatgcaaaga tcgcagccct cggattatta ttgactgaaa tgatgcaatc tgcatcaatt
      901 cctgtcatat gctgaattgc cccggcaatc ccaaatgcaa gatacagttt cggttttacg
      961 gagattcctg actgtcctac ctgctgtgtt ggaagcatcc atccggcatc tacacacggt
     1021 cttgtacatg caacttcccc tccgatcttc tctgcaagtc tctctagaag cgcaaatcct
     1081 tcttctcctt tcattccacg accaccggaa actatgatcc ttgctgtatc cagatgaact
     1141 tcttttccac tttttcctgc cgctccatga tagatcagat cttgtcgttc ttccttcaga
     1201 cattctggat gcacatataa catctcacct tgtttgtctt ccttgtattc tgcttttccc
     1261 atcactcctg tacgaatcgt gatcaccgat gtcaaggatg gattgatcaa aaagtctgac
     1321 atattttttc catcgaatgc cggtcttgtg atgataagtt gttcctgttt ctcatcgtat
     1381 gtaagtttcg atgcatcaca gactaattct gtatcaaatg cataagctgt ctttcctgca
     1441 agaactcttc ctgatgctgt tcctccgatc agtatcactt tcggatcctt ttcttttaaa
     1501 acttctatta ccgcatcatg atgcagctgc atatgtcctt ctattaattt ttcatcttcg
     1561 atacagagga tttcatcaac tccgtaattg taaagcttct ttgcctcttc tttgatccca
     1621 catcctgata gaataactga caatgaaaga tctctttcct tcgaaagttt tcctgctgcc
     1681 ccgatcaatt ccaacgtcag cggcatgatc tctctctttt gaatctctcc aacaacaacg
     1741 attccttctt ttttccctaa attcatccaa acacctccta gatcacgctc ttttgcttta
     1801 acaactgaag aatctgtgcc gccttttctt cttcatcttt tccaccgatc ataacaagtt
     1861 tttgctgttt gctgcttttg acttcatatt ttctaagttg cgttactttt ctcttggtca
     1921 cagaaaaatt caggtcttca ttcgtcaata tctttgtctt atatgttcca tcatatgttt
     1981 tcatgatatc tggtacggat ggaaaacggt tcttgttctt ctctctgata gaaagtatca
     2041 ttgctggagt ttttgcttct accttatatg ccatctgatc acccatacat gttgcgaaaa
     2101 cacttccatc tttcatctct gtgatctcgt cagaatacgc gatcaatggg atctccaatg
     2161 cacaagaggt catagaagcc atatgggcac tgtctccatc aattgcctgc cgtccgcata
     2221 agaccagatc atatggacca agctttttga tcaccgctgc gacgatcttg gctacctgtg
     2281 atacatccat atctgcgaat tgggaatcac tgatcaggaa ggcatcatca attccataag
     2341 taagcgtctc tttcataaca tcttctgcag ataatggtcc aaatccgatc acatctacag
     2401 ttccaccata gcggtctctt aaatccagtg cttcttttat tacgttctta tcactgtcat
     2461 ttaactgatc cacatcctga tattcatctt tgatcgaatc atctggaatt tgttttaaaa
     2521 tcacaacaaa tctcatttcg tttctccttt ttgtttattt tgtaaatatt tcgcacgcta
     2581 attatatttc acattgtaac attctaacaa tttttcgtca agtgttttta ctttccataa
     2641 gacaaagaaa tgggcggcgt aaagaccgtc catttctctg ttcttaggat ttttaatttt
     2701 ttgagtttta tgttttagga gttttcattt ataacttcat ttatacttta tttttctttg
     2761 atcgctttaa tcgcgtcatt taattttgg
//
LOCUS       DAOMUE010000276         2759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10514_length_2759_cov_2.471154, whole genome shotgun sequence.
ACCESSION   DAOMUE010000276 DAOMUE010000000
VERSION     DAOMUE010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10514_length_2759_cov_2.471154"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..>933)
                     /locus_tag="PLC71_09855"
     CDS             complement(63..>933)
                     /locus_tag="PLC71_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM02981.1"
                     /translation="KRGELVGFSRCFGYDRDKETGELKIVESEAETVRHIFDRYLQGA
                     GGTVIARELNEEGILTYNHCQWTCSIILNIIKNEKYMGDMLQGKTYTVDPISKRRLPN
                     NGEEDKFYVEGHHEPIVSRKVFDKAQELRISRNVKRAKTSTESNRVRIRRQYAFSCML
                     QCYFCGSNLSKRTWHSSSKYSKVIWQCVKSAKKGKRFCPESKAIPEAVIEKAFVESYK
                     ILCENNQDILEDLLDKIEVILKDEKIEKEVKQIEGRIKRTKTKRNKLADGYLDGIIPQ
                     ERLRNYFLQSLSF"
     assembly_gap    934..986
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<987..1225)
                     /locus_tag="PLC71_09860"
     CDS             complement(<987..1225)
                     /locus_tag="PLC71_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM02982.1"
                     /translation="MTGTSVRPREDFQRLIEDCMDGKIDMIITKSIPRFARNTLDTLR
                     YVRLLREKNVAIYFEVEKINTLVDGEFLMTILSSVA"
     gene            complement(1256..1432)
                     /locus_tag="PLC71_09865"
     CDS             complement(1256..1432)
                     /locus_tag="PLC71_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02983.1"
                     /translation="MEVKIIQGNKSVLGAFHRKVKKLRVAAYCRVSTDDEDQIKSYNS
                     MIKYYTDLIQKNEE"
     gene            complement(1494..1643)
                     /locus_tag="PLC71_09870"
     CDS             complement(1494..1643)
                     /locus_tag="PLC71_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HRM02984.1"
                     /translation="MEDDEILKYSMQIAMLKQLLNKKLIDEKEYEKIKKELMKEYGVL
                     SDLVI"
     gene            complement(1792..1941)
                     /locus_tag="PLC71_09875"
                     /pseudo
     CDS             complement(1792..1941)
                     /locus_tag="PLC71_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392203.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
     gene            complement(1978..2664)
                     /locus_tag="PLC71_09880"
     CDS             complement(1978..2664)
                     /locus_tag="PLC71_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HRM02985.1"
                     /translation="MDNVERLMEFGLTRHEASLYILLTLEGSLNGYEAAKQSGISRSN
                     AYNALAGLVDKGAAYIQEEDTVRYTPVAIEEFCSNKIRHLEQKKTELIAELPDKRKNE
                     GGYLTIKGEEHICDKIIDMLLCAEERAYISVYNERLEMFREHLEKMVSEGKKVVIITN
                     EPFDLQGATVYLTECKKEQIRLITDSKDVLTGAITNRYEATSLYSSNNNLVEVFKDAL
                     ANEIQLLKMK"
BASE COUNT          712 a          580 c          344 g         1070 t
ORIGIN      
        1 taattggaaa ttgaaaacca attttagaca gacttagatc tgtcttattg tgatgcctaa
       61 agttaaaaag aaagcgattg aagaaaataa tttctcaatc gctcttgcgg tattattcca
      121 tccaagtaac catctgctaa cttatttctc tttgtcttag tccttttaat cctcccttct
      181 atttgtttca cttctttttc tattttctca tcctttaaaa ttacctctat cttatctaat
      241 aaatcttcca aaatatcctg attattttca cataatattt tatatgattc tacaaatgct
      301 ttttctatta ccgcctctgg tattgctttt gattcaggac aaaatctttt tccttttttc
      361 gcagatttta cacattgcca tattactttt gaatatttcg aactgctatg ccaagttctt
      421 tttgataaat ttgacccaca aaaataacac tgcaacatac agctaaatgc atattgtctt
      481 ctgattctaa ctctattcga ttctgttgat gtctttgctc gttttacatt tcttgatatt
      541 cgcaattcct gtgctttgtc aaaaaccttt cgtgatacaa tcggttcatg atgtccttct
      601 acataaaact tatcttcttc tccattgtta ggcaatcttc tctttgatat aggatcaacc
      661 gtataagtct ttccctgtag catgtctccc atatattttt cattttttat gatatttaaa
      721 attatcgaac atgtccattg acaatgatta tacgtgagaa ttccttcttc attcaattct
      781 cttgcaatta cagttcctcc agctccttgt aaatatctat cgaaaatatg ccttacagtt
      841 tctgcctctg attctacaat tttcaattca cccgtttctt tatcacgatc atatccaaag
      901 catcgactaa atcctaccaa ttcacctctt ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnngcaa cagaacttag gattgtcatt agaaattcac
     1021 cgtctaccag cgtattgatc ttttcaactt caaaataaat agctacattc ttttctctta
     1081 acaaacggac atatcttaaa gtatccaatg tatttcttgc aaaccttgga atactctttg
     1141 taatgatcat atcaattttt ccatccatac aatcttctat gagtcgttga aaatcttctc
     1201 taggtcttac acttgttcct gtaatagcct tatcagcata aattccaaca aattcctatt
     1261 cttcattctt ctgaattaaa tcagtataat atttaatcat agaattataa cttttaatct
     1321 gatcttcatc atctgtgctg actctacaat atgctgcaac tctaagtttc ttaacttttc
     1381 tatgaaaagc tcctaaaact gatttgtttc cttgtataat ttttacttcc attttctttt
     1441 tatcctttgc tatgttgtaa tgctacacat tctaatgaag tcaatttttc acgtcaaata
     1501 actaaatcag acaaaactcc atattctttc atcaattctt tttttatctt ttcgtattct
     1561 ttctcatcaa ttaatttttt gttcaataat tgctttaaca ttgctatttg catgctgtat
     1621 tttaatattt catcatcttc cataaatcag ttccttccta ttaaatagaa tattaatcct
     1681 acaagcatcc ctggaatcag gaccgcattg gaatctcacc atctctatgg actgatctat
     1741 atcagaagta tcattattaa aaggcaacat cttcgccaaa gattttccat tttctttaaa
     1801 tcctttattc catgaaaatg ctttgtataa tcctcttgct gtttcacgga ttcctttttc
     1861 atcatataaa tgaaatggtg ttggatattt ttttacgatt tcatctaact gttcttttgt
     1921 tacaaatggt acttttgcca tggtgtttgg ttcctttctt ctatactttt tgtatattta
     1981 tttcattttt aatagttgta tttcattggc cagtgcatct ttaaatactt ccaccagatt
     2041 attgttactg gaatacaggc tggttgcttc gtaacgattt gtgatcgcgc ctgttaaaac
     2101 gtctttggaa tccgtgatca atcggatctg ttctttctta cattctgtca gataaacagt
     2161 tgctccttgt aaatcaaatg gttcattcgt tatgatcacg actttctttc cttcacttac
     2221 catcttctct aagtgttccc gaaacatctc cagtcgttca ttgtatacag agatatacgc
     2281 acgctcttct gcacaaagca gcatatcaat gatcttatcg caaatatgtt cttcgccttt
     2341 gatcgtcaga tacccgcctt cgttcttgcg tttgtccgga agttccgcga tcagctctgt
     2401 cttcttctgt tccagatgcc ggatcttatt actgcagaat tcttcgatcg ctaccggtgt
     2461 gtaacgaact gtatcttcct cttgtatata ggctgcgcct ttgtctacca agcctgccag
     2521 tgcattgtaa gcgttcgatc ttgagatccc gctttgtttg gctgcctcgt atccgttaag
     2581 gcttccttcc agtgtcagta aaatgtaaag actggcttcg tgtcttgtaa gtccgaattc
     2641 catcagacgc tctacattat ccatcacttt cacctcgtgt tcctatctag gactactata
     2701 gtcgaattaa aaaagaatgt caaggtttcc ttgacattct gagaattttt cttgagttt
//
LOCUS       DAOMUE010000277         2748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10573_length_2748_cov_3.044189, whole genome shotgun sequence.
ACCESSION   DAOMUE010000277 DAOMUE010000000
VERSION     DAOMUE010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10573_length_2748_cov_3.044189"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLC71_09885"
     CDS             complement(<1..210)
                     /locus_tag="PLC71_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HRM02986.1"
                     /translation="MEKAVSWKKILILAIAVCLFAGIIVKCNQFQKTPLASYKGQKYE
                     KAVVTKIIKDNLAEDGSRYGTQKVLV"
     gene            complement(351..2465)
                     /locus_tag="PLC71_09890"
     CDS             complement(351..2465)
                     /locus_tag="PLC71_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM02987.1"
                     /translation="MRTIRRNIARNAKKYAAKATVSAMMLAGGVMMASPILSSTVSAA
                     TEHWNDASASKTESWSTWKANWDKYSTNYENVSLTPGATESQLNFAYYSKTEETPKVK
                     IATKADMSDAKEVEGKQTQAVAIEGVQYYSNKASVNDLKENTTYYYQVFQNGKYQDVQ
                     KYSTKSFKNYSFLYVGDPQIGASSGQTSTEGDAMKDNNYAARNDSYNWNNVLNNAVKQ
                     NPNLSFVASAGDQVNNNNNEKQYAGYLGADALRSLPVATTIGNHDSGSAQYEMHYNNP
                     NAFDTSGYRNTAKYTEGKTAAGTDYYYTYGNTLFIVLDTNNYNCATHENVMRKAIKEN
                     PNAKWKVVMFHQDIYGSGYDHSDSDGMVLRTQLTPLMDKYDIDVVLQGHDHTYSRTYQ
                     LQSDGQAHDKFAKTENTANYAKENNCYEIVDTTKGGTVVNPKGTVYLEANSATGSKFY
                     NLITAKQDFISERSQTWTPSYSVVNVTDDSFEVTTYDADTGKVLDGSSSYKIVKKAED
                     TKKDDANSNTTKKDDTTAVQAKNQTITATASYKKSETSKAFKLNAKTNGNGKLTYTTS
                     NKAVATVDAAGKVTVKGLGVAKITVKAAATTDYKAASKTVTVTVAPKKQGISLVNKIK
                     KQLTIKWKKNTKASGYQVVYSTNKKFTGKKTVRKAKTTTSYKIKGLKKGKKYYVKVRS
                     YKTVNGKRIYGAYSTVKKATIK"
BASE COUNT          633 a          494 c          487 g         1134 t
ORIGIN      
        1 aaccagaact ttctgcgttc cataacgact tccgtcttct gcaagattat cttttatgat
       61 ctttgttaca actgcttttt catatttttg tcctttataa gatgccaatg gcgttttctg
      121 aaactgattg catttgacta tgatccctgc aaataaacac actgctattg cgaggatcaa
      181 gatttttttc catgatactg ctttttccat aagttgtctt ctttctataa atcgtagcct
      241 tttatttata aagaaaaatc agagaatgcc tgtgtccgga tctatcattc cgggcgcagg
      301 cttttgctct gtctgataaa ttaagttacg acactaattc tttatcatct ctatttgatt
      361 gttgcttttt tgactgtgct gtaggctcca taaatacgtt ttccatttac tgttttgtaa
      421 gaacggactt ttacgtaata ttttttccct tttttcagac ctttgatctt gtaagatgtt
      481 gttgtttttg cttttcttac tgtcttttta cctgtaaatt tcttgtttgt agaatataca
      541 acttgatatc ctgatgcttt cgtattcttt ttccatttga tcgttagctg ttttttgatc
      601 ttatttacta aagagatacc ctgtttcttt ggtgctacag tcactgtaac tgttttggaa
      661 gctgctttgt agtctgtagt tgctgctgct ttgactgtga tcttggcaac tcctagtcct
      721 ttgactgtta cttttcctgc ggcatcaact gttgcaactg ctttgtttga tgttgtgtaa
      781 gtgagttttc catttccatt tgtttttgca tttaatttga atgctttgga tgtttcactc
      841 ttcttataag atgctgttgc tgtaatcgtc tggttttttg cctgtactgc tgttgtatca
      901 tctttctttg ttgtatttga gttcgcatca tcttttttgg tatcttctgc tttcttcaca
      961 atcttatatg aagatgatcc gtctaatact tttcctgtgt ccgcatcgta tgttgtaact
     1021 tcaaaagagt catctgttac attgactaca gaataacttg gtgtccatgt ctggcttctc
     1081 tcagaaatga aatcctgttt tgctgtgatc agattgtaga atttagatcc tgttgctgag
     1141 tttgcttcta aatatacagt tccttttgga tttacaaccg ttccaccttt tgttgtatca
     1201 acgatttcgt agcagttgtt ttcttttgcg taatttgctg tattttctgt ttttgcgaat
     1261 ttatcatgtg cctgtccatc actctgtaac tgatatgttc tggagtatgt atggtcatgt
     1321 ccctgaagta caacatcaat atcatattta tccattaatg gtgttaactg tgttcttaaa
     1381 accattccgt cagaatctga atgatcatat cctgatccat aaatgtcctg gtggaacatt
     1441 acaactttcc attttgcatt tggattttct ttgatcgctt tacgcataac attttcgtga
     1501 gttgcacagt tgtaattgtt tgtgtctaat acgataaaca atgtatttcc atatgtgtaa
     1561 taatagtctg ttcctgctgc tgtctttcct tctgtatact ttgctgtatt tctatatcca
     1621 cttgtatcaa atgcatttgg attgttgtaa tgcatctcat actgtgctga tccagaatcg
     1681 tggtttccga tcgttgttgc tactggaaga gatcttaaag catctgctcc aaggtaacct
     1741 gcatactgtt tttcattgtt attgttattt acctgatctc ctgcggatgc aacaaaactt
     1801 agatttggat tctgttttac cgcgttattt aatacgttgt tccagttata actatcattt
     1861 cttgctgcat agttattatc tttcatcgca tctccttcag tacttgtctg tcctgaagat
     1921 gctccgatct gaggatctcc tacatataag aaagaataat ttttaaatga ttttgtagaa
     1981 tatttctgta catcctgata ttttccgttc tggaatacct gatagtagta agttgtattt
     2041 tctttcaggt catttacaga tgctttgttt gaatagtact gaacaccttc gatcgctact
     2101 gcctgtgtct gttttccttc gacttctttt gcatcagaca tatctgcttt tgttgcgatc
     2161 ttaacttttg gtgtttcttc tgtcttgctg tagtatgcaa aattaagctg tgattctgtc
     2221 gctcctggtg ttagggatac attttcatag tttgtgctgt atttatccca gtttgctttc
     2281 catgtggacc agctttctgt tttagaagca gatgcatcat tccaatgttc tgtcgctgcg
     2341 ctgactgttg atgaaaggat tggagatgcc atcataactc cgcctgctaa catcatggct
     2401 gatacagttg cttttgctgc atattttttt gcatttcttg ctatgtttct gcgaattgtt
     2461 ctcacaataa gatcctcctt tgatatagtc aatcattctc tgttgtgttt aactataaac
     2521 ttgtgagatt aaggaaaggt acgtgggaat gataagtttt tgtaaaattt tttaattgta
     2581 tgacgggaat gtgtcgagta ttcataaaaa gataaagaaa gatagcggta cgaaaaacga
     2641 attccattga tggatgtatg ttaaacgtta tcgtttttta atttttatag aggtggacag
     2701 atattattaa tgctaataca gtaaaacata cacactgtgt agtggttt
//
LOCUS       DAOMUE010000278         2746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10582_length_2746_cov_2.131550, whole genome shotgun sequence.
ACCESSION   DAOMUE010000278 DAOMUE010000000
VERSION     DAOMUE010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10582_length_2746_cov_2.131550"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..375
                     /locus_tag="PLC71_09895"
     CDS             133..375
                     /locus_tag="PLC71_09895"
                     /inference="COORDINATES: protein motif:HMM:NF039998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6120 family protein"
                     /protein_id="HRM02988.1"
                     /translation="MTITRKYIRQCRTLFPVYGNSERTFLNRLKVQINEHLDLFPDLS
                     YEELVKQFGTPKEVIMEYYANADDDYLLKKLMYQKN"
     gene            complement(1107..1670)
                     /gene="pdxT"
                     /locus_tag="PLC71_09900"
     CDS             complement(1107..1670)
                     /gene="pdxT"
                     /locus_tag="PLC71_09900"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HRM02989.1"
                     /translation="MKQVGVLALQGAFIEHEKMLEQLGVSCIELRNKEDLEKPYDGII
                     LPGGESTVQGKLLKELDMFDTIKKQIEAGMPVLATCAGLILLAKDIKDQEETYFGTIP
                     MCVKRNAYGRQLGSFFTTKEVDGVGEVPMTFIRAPYVESVEDGVKVLAEVDGNIVGVE
                     YGKQMGFAFHPELDGDLRIHQRFVDKI"
     gene            complement(1672..2550)
                     /gene="pdxS"
                     /locus_tag="PLC71_09905"
     CDS             complement(1672..2550)
                     /gene="pdxS"
                     /locus_tag="PLC71_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRM02990.1"
                     /translation="METTKRYELNKELAQMLKGGVIMDVTTPEQAKIAQEAGACAVMA
                     LERIPADIRAAGGVSRMSDPKMIAGIQEAVTIPVMAKCRIGHFAEAQVLEAIEIDYID
                     ESEVLSPADDVYHINKRNFKVPFVCGAKDLGEALRRINEGASMIRTKGEPGTGDIVQA
                     VRHMRKMNSQIAQLVSMREDELFEAAKQLRVPYDLVVYVHENGKLPVVNFAAGGVATP
                     ADAALMMQLGAEGVFVGSGIFKSGNPAKRAAAIVQAVTNFTDAKRIAELSKDLGEAMV
                     GINEQEIELLMAERGK"
BASE COUNT          771 a          551 c          447 g          977 t
ORIGIN      
        1 atcaggggaa acgataatgt gtccgtcagg acgtggtact ctgcttagat tttgcttaga
       61 tttatagatg ctattcaatc cgttttgggg cctagagttt agaacagtat atcaaaaagt
      121 gaggaaccta ttatgactat aacacgtaaa tacattcgac aatgtcgtac attattccct
      181 gtttacggga actcagaacg aacttttttg aatcgtttaa aagttcaaat aaatgaacac
      241 ttagacttat ttccagattt aagctacgaa gaattagtaa agcagtttgg aacacctaaa
      301 gaagttatca tggaatatta tgctaatgct gatgatgatt acttgttaaa aaaattgatg
      361 tatcaaaaaa attaaaaaga tttttacttt ttattgcaat attatttctg agttactttt
      421 tttatgaaag ttacacaata tatcaagctc ttaatttgat caaagatcaa caaataatat
      481 atgaaaaaga aggacctgtt gaagaaacaa aataaattag cgaggaattt agtgtgaaaa
      541 aatttataat tttatttttt agtaccttac ttatgttaaa tctattttaa ttgacgattc
      601 agcagatact tcatcatcca aatgtattaa atcagaaatt tccacaactt gccctgcaag
      661 taattggaaa ctttctgaaa aaaagcatac atatctggag catcagctaa agcaactgca
      721 tcttttaaac aatataaaaa agatgtgtat cttcaaacaa ttacaaggac agttacaatt
      781 agttgtaata aatcaggaaa attatattaa tttctttaaa ttatttcctt ttgccacttg
      841 tacacttgat gtatatgata aaacataaat aagaaaccag aatcacagca ttggaatttt
      901 gccatatttt gaatgagttc gcagcccaat tggacgattg tctgagctta gaagtctaca
      961 agtttctact aacttcaaag cgagttttcg taggaaattg ggtggatcag cgactcgttc
     1021 aaaatgtgag taaaattcca ccgctgtgat tctggtttct tatttatgtt ttatcatata
     1081 ctcatagcct acaattggcc aatcaattaa attttatcta caaacctctg atgtatccgc
     1141 aaatccccat ccaattccgg atgaaatgca aatcccatct gtttcccata ttctacccca
     1201 acaatatttc cgtcaacttc tgctaatact tttaccccat cttctacgct ttccacatat
     1261 ggtgcgcgga taaatgtcat tgggacttct ccgactccat ctacttcttt tgttgtaaag
     1321 aagcttccga gctgtcttcc atatgcattt cgtttgacgc acattgggat tgttccaaag
     1381 tatgtttctt cctgatcttt aatatctttt gcaagaagaa taagtcctgc acaggttgcg
     1441 aggactggca ttcctgcttc gatctgtttc ttgatcgtat caaacatatc aagttctttt
     1501 agaagtttcc cctgaacggt gctttcaccg ccagggagga tgattccatc gtatggtttt
     1561 tctaaatctt ctttgtttcg taattcaata caggaaacgc ctagttgttc aagcattttt
     1621 tcatgttcga taaatgcgcc ctgtaatgct aaaacaccga cttgtttcat cttattttcc
     1681 tctttctgcc attaataatt cgatttcctg ttcgttgatt cctaccattg cttccccaag
     1741 gtctttggat aattctgcga ttcgtttggc atctgtaaag tttgtcactg cctgtacgat
     1801 cgctgctgct cgtttggctg ggtttccaga tttgaagatt cctgatccta cgaagactcc
     1861 ttctgctcct aactgcatca ttaaggctgc atccgctggt gttgcgactc cacctgctgc
     1921 gaagtttacg actgggagtt ttccattttc gtgaacgtat actacaagat catatggaac
     1981 tcttaactgt tttgcagctt caaataattc atcttccctc atgctgacta actgtgcgat
     2041 ctggctgttc atctttctca tatggcgcac tgcctgtacg atatctcctg ttcctggttc
     2101 tcctttggta cggatcatgg atgctccttc attgattctt cgaagtgctt ctccaagatc
     2161 ttttgctcca cacacaaatg gaactttgaa gtttctttta ttgatatgat atacatcatc
     2221 tgctggtgat aatacttcgc tctcatcgat atagtcaatt tcaattgctt ctaaaacctg
     2281 tgcttctgca aaatgtccga ttctgcattt tgccatcact ggaattgtga ctgcttcctg
     2341 aattcctgcg atcatttttg gatcactcat tcttgatact ccacctgctg ctctgatatc
     2401 tgccggaatt ctctctaatg ccataactgc gcatgctcca gcttcctgtg cgatctttgc
     2461 ctgttctggt gttgtaacat ccatgatcac tccacctttt aacatctgtg ccaattcttt
     2521 atttaattcg tatctctttg ttgtttccat gttgtaagcc tccactgtat ttttctaaat
     2581 tcatttgcta tgttttcttt acatatgcta tgggactgat tataattaat ttgtggcctt
     2641 tttaaaatca tcaatttgaa tatgttcaat aaggccagat tggagaattt acatatcatg
     2701 ttaacctata actttgcaaa taaagggtca gattcccttt acgaat
//
LOCUS       DAOMUE010000279         2743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10594_length_2743_cov_2.018229, whole genome shotgun sequence.
ACCESSION   DAOMUE010000279 DAOMUE010000000
VERSION     DAOMUE010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10594_length_2743_cov_2.018229"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLC71_09910"
     CDS             complement(<1..385)
                     /locus_tag="PLC71_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HRM02991.1"
                     /translation="MKKYMVWLISALMAVSMTACSNAQKKDTKDQAQITTAAKENETQ
                     TEAAKTTSKKEKKNEKKKQKSKKNSKKTKNSTAKKKETSTTTAKKKKVKKKKTKEKTT
                     KNTKETTKTTTASKNESTVQTAENQT"
     gene            complement(711..1484)
                     /locus_tag="PLC71_09915"
     CDS             complement(711..1484)
                     /locus_tag="PLC71_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRM02992.1"
                     /translation="MLRKITIMIFLILTVVWFTNSDSIQPAREFIQNNIYVWSEMQEE
                     KLPIYCVDTQKKQIALTFDTAWGNEDIPQILKILKQKNVKATFFFCGDWISKYPADIK
                     TIYEEGHDIASHGDHHKYMTKLTDKQQQEEIQGVTQKIQGLLGIKIDLFRAPYGDYNE
                     SVVRNARKMNYYMIQWNVDSLDWQEPTKEQLIKKVCEHKNLSPGSIILMHTGTKCTKQ
                     ALKQIIKNIKAKGYEFVPVSRLIYRRDYRIDPTGKQMKL"
     gene            complement(1582..2694)
                     /gene="nagA"
                     /locus_tag="PLC71_09920"
     CDS             complement(1582..2694)
                     /gene="nagA"
                     /locus_tag="PLC71_09920"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRM02993.1"
                     /translation="MIIKNGLVFTPEGKFEKKDLYVEGEYVAKETTDNKEVEAEGCYV
                     IPGLVDIHFHGCVRHDMCDGTEESIQALADYEAANGVLAICPATMTIPEEELFQAMKA
                     AKGHKNGKGADLVGINMEGPFINKEKKGAQKEEDIKLADVELFHKLQEAAGGLIKLVD
                     LAPETEGAMDFINQVKDEVHVSIAHTMADYDTASEAIRRGADHITHLYNAMPPLNHRE
                     PGVIGAARDSDCYVELICDGVHIHPSCVRATFEMFTDKRIVLISDSMMATGMEDGQYE
                     LGGQPVTVVGNVATLTEGGAIAGSATNLMDCMRTVVKEMHIPFESAVRCATLNPAKSI
                     GIDDKYGSLEEGKYANAVVLDKDLNIVSIIQKGQVK"
BASE COUNT          607 a          589 c          422 g         1125 t
ORIGIN      
        1 ttgtctgatt ctctgccgtc tgtactgttg attcattttt actggctgtt gttgtttttg
       61 ttgtttcttt tgtattcttt gttgtctttt cttttgtttt tttcttcttt acttttttct
      121 ttttagctgt tgttgtgctt gtctctttct tttttgctgt actgtttttt gtctttttgc
      181 tgtttttctt actcttctgt tttttctttt cgttcttctt ttctttttta ctcgttgttt
      241 ttgctgcttc tgtctgcgtt tcattttctt ttgcagctgt tgttatctgt gcctgatctt
      301 ttgtatcttt cttctgtgca tttgaacagg ctgtcatcga tactgccatc aatgcagaaa
      361 tcagccatac catatatttt ttcataaaaa atagatcctc ccacgtatac agcataaaag
      421 gatctactct ggcttattta cataacaaaa agacatgcat tcttattcat ttctaaaaag
      481 actacatttt atcatcttat tttttgtcat tctggcacca tgaggacgac ttcgtcactc
      541 gcaaagtata tccttagatc atggcaagta ttccggctta agatctgcat atacagatta
      601 ccatgaaatt atgcgtttct tttcattatg tattatttgt tatttgattg tcaagatttt
      661 cgagtgtttt aataggattt cgatgcttgt ttcgatacct ctttgatatt tcatagtttc
      721 atctgtttcc ctgttggatc aatacggtaa tctcttcgat agatcaatct tgacactggt
      781 acgaactcat atccttttgc tttgatattt ttaatgatct gttttaatgc ctgtttggta
      841 catttcgttc ctgtatgcat cagaatgata cttccaggtg ataaattttt gtgttcgcaa
      901 actttcttaa ttaactgttc tttcgttggc tcttgccagt ctaggctgtc tacgttccat
      961 tggatcatat aatagttcat ttttcttgca ttccttacga cggattcgtt gtaatctcca
     1021 tatggtgccc ggaaaagatc tattttgata cctaacaatc cttgaatctt ctgtgtcact
     1081 ccttgaattt cttcctgctg ctgtttgtct gttaattttg tcatgtattt atgatgatct
     1141 ccatgacttg caatatcatg tccttcctca tagattgttt tgatatcagc tggatatttg
     1201 ctgatccagt ctccacagaa gaaaaaggtt gcttttacat ttttctgttt taatattttg
     1261 agaatctgtg gtatatcttc attcccccat gcagtatcaa atgtcagtgc tatttgtttt
     1321 ttctgcgtat ctacacaata gattggaagt ttttcctctt gcatttcact ccaaacataa
     1381 atgttgtttt gtataaattc tctggcgggc tggatactgt cactgtttgt aaaccagact
     1441 actgttaaga tcagaaagat catgatcgtg atttttcgaa gcatttttct gctccctttg
     1501 tttttatcat tttatgaatt cctgtataaa aaaagaatcc tgcctacaca gaattctttt
     1561 tcttatctca attgatattc tttattttac ctgccccttc tgaatgatgg atacaatatt
     1621 taaatcttta tctaaaacta ctgcattcgc atattttcct tcttcaaggc ttccatattt
     1681 atcatcaatt ccaatggatt ttgctggatt caaagttgca catcggactg cactttcgaa
     1741 tggaatatgc atttctttga ctaccgttct catacaatcc attaggtttg ttgcagaacc
     1801 tgcgatggct cctccttcgg tcagtgttgc aacgtttcct actacagtca ctggctgtcc
     1861 gcctaattca tactgtccat cttccattcc cgttgccatc atactgtcac tgatcagtac
     1921 gatcctcttg tctgtaaaca tttcaaaggt tgctctgaca caggatgggt gaatatgaac
     1981 tccatcgcag atcagttcta cataacaatc actgtctctt gctgctccaa taacccccgg
     2041 ttctctatga tttaacggcg gcatagcatt atacaaatgt gtgatatgat cggcacctct
     2101 tctgattgct tcacttgctg tatcataatc agccatcgta tgtgcaattg atacatgtac
     2161 ttcatctttc acttgattga taaaatccat agctccttca gtctctggcg caagatccac
     2221 taatttgatc aatccaccgg ctgcttcctg cagtttatgg aataattcaa catccgccag
     2281 cttgatatct tcttctttct gggcaccttt tttctctttg tttataaaag gcccttccat
     2341 attgatcccg acaagatctg caccttttcc attcttatgt ccttttgctg ccttcattgc
     2401 ctgaaatagt tcttcttctg gaatcgtcat tgtcgcagga cagattgcca atactccatt
     2461 ggctgcttca taatcagcca gtgcctgaat ggattcttcg gttccatcgc acatatcatg
     2521 tctgacacat ccatgaaaat gaatatcgac aagtcccggt atcacataac atccttcagc
     2581 ttctacttct ttgttgtccg ttgtttcttt cgctacgtat tctccttcta catagagatc
     2641 ttttttctca aattttcctt ctggtgtgaa taccagtcca ttttttatga tcattatatt
     2701 acctcgattt ccgttaccca tttgtattgt tctttctgat tct
//
LOCUS       DAOMUE010000280         2739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10615_length_2739_cov_2.241058, whole genome shotgun sequence.
ACCESSION   DAOMUE010000280 DAOMUE010000000
VERSION     DAOMUE010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10615_length_2739_cov_2.241058"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="PLC71_09925"
     CDS             <1..1172
                     /locus_tag="PLC71_09925"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRM02994.1"
                     /translation="VKQIRLPLEVDKMNALLGTNVSKEEVLEYFGRIDLGYDEATNEV
                     IVPSFRQDIHRMADLAEEVARFYGYDNIPVTLPNGESTAGKKPFKIRVEDVCRNVAEQ
                     NGFCGGMTYSFESPKVFDKLLLDENAKERQAIEISNPLGEDFSIMRTVSLNGMLTSLS
                     TNLAHRNKNVRLYEFGNIYIPKSLPLTELPDERMQMTLGMYGECDFFTLKGVVEDMLD
                     SLGLGGRSEYAPTGEHPFLHPGRAADVTIDGEKIAYIGQIHPEVMDNYNMKGEVYVAV
                     IDMPTLVPKATFDRKYEGVAKFPAMKRDLSMVMKKDIFVGQLEKIFKDKGGKLLESYE
                     LFDVYEGDQIEKGYKSVAYSLTFRAKDRTLEDAEVSKIVEKILDELKKLGVELRA"
     gene            1355..2326
                     /locus_tag="PLC71_09930"
     CDS             1355..2326
                     /locus_tag="PLC71_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02995.1"
                     /translation="MKSNINKLILIEVIAGILFAFIASKILHMTAALQIMYLPFELIG
                     KGLRFLSLSSAIGNVIAIIIYAAISMIPAIYIIYQRKRGRTQKEDILLVAFTGLLFYS
                     MYMFINPGLMYQKMPDIFSMDQNSLIVMKIIYAFIIYSVLIMWLIMKAIRFLGDDSQK
                     NWKSRLYFGMKGLIAALVSYYTLLITYAKTFELLHKSENKLQNVSSIEWFKSVLQLLP
                     IIMTILLFINIIHVVKAIEDHKEEQQMMTVARLASVAKVTAVVTVISDFILNFYQFLL
                     GATLQNVNVTIATDVSMEPLIIAFGALIFHEYFKRTKQLKEENDQFI"
     gene            2341..2565
                     /locus_tag="PLC71_09935"
     CDS             2341..2565
                     /locus_tag="PLC71_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRM02996.1"
                     /translation="MAIKIHLDDILKERNMTSKELCKKVHITEANLSILRSGKAKGVR
                     FSTINKICYYLECDVGDILKFDGRLEEEDE"
     gene            2558..>2739
                     /locus_tag="PLC71_09940"
     CDS             2558..>2739
                     /locus_tag="PLC71_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM02997.1"
                     /translation="MSRGIRKTGIFIAIMLTVVVLTGCQIAKKDESGQAKEELYGAYA
                     VVGDRIPDGKIAGVKD"
BASE COUNT         1006 a          450 c          557 g          726 t
ORIGIN      
        1 cagtcaaaca gatccgcctt ccattagaag tagacaagat gaatgccctt cttggaacaa
       61 atgtatccaa agaagaagtc ttagaatatt tcggaagaat cgaccttgga tatgatgaag
      121 caacaaatga agtcatcgta ccatccttcc gtcaggatat tcaccgtatg gcagaccttg
      181 cagaagaagt tgcaagattc tacggatatg ataacatccc agttacatta ccaaacggag
      241 aatccacagc cggtaagaaa ccattcaaga tccgtgttga agatgtctgc agaaatgtag
      301 cagaacagaa cggattctgt ggtggaatga catattcctt cgaaagtcca aaagtattcg
      361 ataaattatt attagatgaa aatgcaaaag aacgtcaggc aatcgagatt tcaaacccat
      421 taggagaaga cttcagtatc atgagaacag tttccttaaa cggaatgtta acatctctgt
      481 ctacaaactt agcacacaga aataaaaatg taagattata tgaatttgga aatatctaca
      541 ttccaaaatc attaccatta acagaattac cagatgaaag aatgcagatg acacttggaa
      601 tgtacggaga atgcgatttc ttcacattaa aaggtgttgt agaagatatg cttgattcct
      661 taggattagg tggaagatct gaatatgcac caacaggtga acatccattc ttacacccag
      721 gaagagcagc agatgtgacg atcgatggag aaaaaattgc ctacatcgga cagatccatc
      781 cagaagttat ggataactat aacatgaaag gtgaagtcta tgtagcggtc attgacatgc
      841 caacattagt accaaaagca accttcgacc gcaaatacga aggagtagcc aaattcccag
      901 caatgaaacg tgacttaagt atggttatga agaaagacat cttcgttgga cagcttgaga
      961 agatcttcaa agataaaggt ggaaaacttc tcgaatccta cgaattattc gatgtctacg
     1021 aaggagacca gatcgaaaaa ggatataaat ccgtagcata ctccttaaca ttcagagcaa
     1081 aagaccgtac attagaagac gcagaagtat ctaagatcgt tgagaagatt cttgatgaac
     1141 tgaagaaatt aggcgttgaa ttacgtgctt aattgtgatt gaaaataaaa taaagtgaga
     1201 attaaaatcc tctggcagat aatagtgtca ggggattttt gtgtatcaga aagaagcaat
     1261 atgtaaaggt gttgaaaaaa ttattgtttt tcaataattt ttatgctaca ataatcttga
     1321 aattatcgaa aaacaataat tggaggtaga taggatgaaa tcaaacataa ataaactaat
     1381 cctaatcgaa gtcatcgcag gaatcctttt tgcattcata gcaagcaaaa tcctgcatat
     1441 gacagcagca ctacagatta tgtacttacc atttgaactc ataggaaaag ggctaagatt
     1501 tctatcccta agttcagcaa taggaaatgt catcgcgatc atcatatacg cagccatttc
     1561 aatgatacca gcaatctata tcatctatca gagaaaaaga ggaagaacac agaaagaaga
     1621 catattactt gttgcattca cagggctatt attttattcc atgtatatgt tcatcaatcc
     1681 aggactcatg tatcagaaaa tgccagacat tttttcaatg gaccagaata gtctgattgt
     1741 aatgaaaatt atttacgcat tcatcattta cagcgtcctg attatgtggt tgatcatgaa
     1801 agcaatccgt tttcttggag atgacagcca gaagaattgg aaaagccgct tatattttgg
     1861 aatgaaagga ctcattgctg cgctagtcag ttattacacg cttttgatca catatgcaaa
     1921 gacatttgaa ctgcttcata agagcgaaaa taaactgcaa aatgtatctt caatagaatg
     1981 gtttaaatca gtattacagt tattaccaat cattatgaca atcctgctat ttatcaacat
     2041 cattcatgta gtaaaagcaa tcgaagatca taaggaagag cagcagatga tgacagtggc
     2101 aagacttgca agtgtcgcaa aggttactgc agtggttaca gtcatatcag attttatcct
     2161 gaatttttac cagtttttac taggagcaac cttacagaat gtaaatgtta cgattgcaac
     2221 agatgtatcc atggaaccat tgatcatagc atttggagca ttgatcttcc atgaatactt
     2281 caagcggact aaacagttaa aagaagaaaa tgaccagttt atatagaagg aatgatagtt
     2341 atggcaatta aaatacattt ggatgatatc ttaaaagaac gaaacatgac atccaaagaa
     2401 ttatgtaaaa aagtccatat tacagaagca aacctgtcaa tcttaagaag tggcaaggca
     2461 aagggagtgc gtttctcaac gatcaataag atttgctatt atcttgagtg cgatgtcggg
     2521 gatatcttga aatttgacgg gagattggag gaagaagatg agtaggggaa tcagaaaaac
     2581 aggaatattt atagcgatta tgcttacggt agtagtctta acaggatgcc agatcgcaaa
     2641 gaaggacgaa agtggacagg cgaaggaaga attatatgga gcttatgcag ttgtagggga
     2701 caggatacca gacggtaaga tagctggagt gaaagataa
//
LOCUS       DAOMUE010000281         2706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10801_length_2706_cov_2.725009, whole genome shotgun sequence.
ACCESSION   DAOMUE010000281 DAOMUE010000000
VERSION     DAOMUE010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10801_length_2706_cov_2.725009"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..2290)
                     /locus_tag="PLC71_09945"
     CDS             complement(125..2290)
                     /locus_tag="PLC71_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HRM02998.1"
                     /translation="MAKKKKSSAFAVVQQIGKSFFLPVSVLPIAGILLGLGSSFTNAD
                     TIAAYHLQGIMGEGTILNALLTIMSQVGNTIFGNLPLIFALAVALGMAKNEKAVAVLS
                     AGISFFVMNSTINAMLKLSGKILADGTYAKSVLNGQITSVCGIDSLQMGVFAGVIVGL
                     VTAALHNRFYKQQLPAALAFFSGVRFVPIISVIAHIGVGIICFFVWPTIQSGIFALGN
                     LVMHSGYFGTAIFGFLERALIPFGLHHVFYLPFWQTSLGGTMEVAGKMVEGAQNIFFA
                     QLADPNTKHFAVEACRFMTGKYSFMMAGLPGAAYAMYRCAKPEKRKIVGGLLFSAALT
                     SFLTGITEPIEFTFLFIAPGLFILHCGLAGLSFALMHILKICIGTTFSCGLIDFMLYG
                     VLQGQTKSNWMMILPVFAVYAVLYYFVFKFVIEKFDLPTPGRDDDEEEVKLYTKADYQ
                     AKKGAANEDTDAPEDPISFMILKGLGGIKNIEDIDCCATRLRITVTDETKVTDQYLKQ
                     SGSKGIIKKGTGIQIIYGPQVSVIKSNFEEYVEYYAQHGTDPSKEEEVTPIVSSKEEE
                     KKEIRHGKLVAVATGKVLAMTQAKDEAFATCAMGDGVVIEPEKGVVVAPADGKITALM
                     KPSLHAIGIETKEGLNLLIHIGIDTVKLDGKGFRAFVKSGQEVKAGDKLVEFDIDLIK
                     KEGYSPDVMVVVLEDSNLPKVAYKTGEKTVAGKTVVAEF"
     gene            complement(2292..>2706)
                     /locus_tag="PLC71_09950"
     CDS             complement(2292..>2706)
                     /locus_tag="PLC71_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HRM02999.1"
                     /translation="EIRKVEHTPMDFREWKKIKNDLYAEDEQLEKGKGYDHNFVIRNK
                     DCGFRKMAEALDEEQGIKMEVYSDLPGLQFYTGNTMKSTKGKGGVIYGKGCGFCMEPH
                     YFPNSINTKEFDAPVFDAGEVYQTVTMYQFVTKED"
BASE COUNT          763 a          596 c          406 g          941 t
ORIGIN      
        1 cgcggtctcg accttggcaa ggtcgcgcgt taccaactac gccactatcg catataatga
       61 ctgttccttt ttcagaacag tcattatttt accttattca cagtttgttg tcaatcattt
      121 tttactaaaa ttccgcaaca actgtttttc ctgcaactgt tttttctcca gttttataag
      181 ctacttttgg cagattagaa tcttctaaga caacaaccat aacatctggg ctatatcctt
      241 ctttcttgat cagatcgata tcaaactcta ccaacttatc tcctgccttt acttcttgtc
      301 cactcttgac aaaagcccta aatccttttc catctaattt gactgtatcg attccgatat
      361 gaattaaaag atttaatcct tcttttgtct cgattccaat cgcatgtaaa gatggtttca
      421 ttaatgctgt aatctttcca tctgctggtg caacaacgac acctttttct ggttcgatca
      481 caacaccatc tcccatagca caagttgcaa atgcctcatc ttttgcctgt gtcattgcaa
      541 gaacttttcc tgttgctacc gcaactaatt taccatgtct gatttctttt ttctcttctt
      601 ctttggagga aactatgggt gttacctctt cttctttgga aggatctgtt ccatgctgtg
      661 cataatattc cacatattct tcaaaattac tcttgatcac agatacctgt ggtccataaa
      721 taatctgaat tcctgttcct tttttgatga ttcctttgga accagattgt tttaagtact
      781 gatctgtcac cttagtttca tctgttactg tgatacgaag tcttgtagca cagcagtcaa
      841 tatcttcaat atttttgatt ccaccaagtc cttttaagat cataaaggaa attggatcct
      901 ctggtgcgtc tgtatcttca tttgctgcac ctttttttgc ctggtaatca gcttttgtat
      961 ataattttac ctcttcttcg tcatcatctc tacctggtgt tggaagatca aatttctcaa
     1021 tgacaaattt gaacacaaag taatataata ctgcataaac tgcaaatact ggaaggatca
     1081 tcatccagtt actctttgtc tgtccctgta atacaccgta taacataaag tcgatcaatc
     1141 cacaagagaa tgttgttccg atacagatct ttaagatatg cattaatgca aatgacaaac
     1201 ctgctaatcc acaatgtaag ataaataatc ctggtgcaat aaataagaat gtaaattcaa
     1261 tcggttctgt aattcctgta aggaatgatg ttaatgctgc tgagaatagt aatcctccaa
     1321 caatttttct tttttctggt tttgcacaac gatacattgc ataagctgct ccaggaagtc
     1381 ccgccatcat gaaagaatat tttcctgtca tgaatctaca tgcttctact gcaaaatgtt
     1441 ttgtatttgg gtctgccagc tgtgcaaaga agatattctg tgcaccttct accatctttc
     1501 cagcaacttc cattgttcct ccaagagatg tctgccagaa tggtaaataa aatacatgat
     1561 gtaatccgaa tggaattaat gctctctcta agaatccaaa gattgctgtt ccaaaatatc
     1621 cagaatgcat aaccagatta ccaagtgcaa agattccact ctgaatcgtt ggccatacaa
     1681 agaagcagat aatacctaca ccgatatgtg cgattacact aataattggc acaaaacgta
     1741 ctcctgagaa aaatgctaat gctgctggta attgctgttt ataaaagcga ttatgtaatg
     1801 ctgctgttac aagtcctacg ataactccag caaatactcc catctgaaga gaatcaatac
     1861 cacagacact cgtgatctgt ccgtttaaga cactctttgc atatgttccg tccgctaaaa
     1921 tctttcctga taattttaac atcgcattaa ttgttgaatt cattacaaag aatgaaatac
     1981 ctgcagaaag tactgctact gctttttcat tttttgccat tcctaatgct actgctaatg
     2041 caaagatcaa tggtaaattt ccaaagattg tattaccgac ctgtgacatg atcgttaaaa
     2101 gcgcattcag gatagttccc tctcccatga ttccttgtaa atgatacgct gcgatcgtat
     2161 cagcattagt aaatgaagat cccagtccta gtaagatacc tgcaattggt aacacagata
     2221 ctggaaggaa aaatgatttt ccgatctgtt gtactaccgc aaatgcggaa cttttctttt
     2281 tctttgccat tttaatcctc ctttgtgaca aattgataca tggttactgt ctgatacact
     2341 tctcctgcgt caaatactgg tgcatcaaac tcttttgtgt taatactgtt tgggaaataa
     2401 tgtggttcca tgcaaaatcc acatcctttt ccataaatca ctcccccttt cccttttgtt
     2461 gatttcattg tatttccagt gtaaaactgt aatcctggaa gatctgaata aacttccatt
     2521 ttaataccct gttcttcgtc caatgcttcc gccatttttc ggaaaccaca atctttattc
     2581 cgaattacaa aattatgatc atatcctttg cctttttcta gttgttcatc ttcagcatat
     2641 agatcattct taatcttttt ccattcacgg aaatccatcg gtgtatgttc tacttttctg
     2701 atttct
//
LOCUS       DAOMUE010000282         2698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10843_length_2698_cov_2.277336, whole genome shotgun sequence.
ACCESSION   DAOMUE010000282 DAOMUE010000000
VERSION     DAOMUE010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10843_length_2698_cov_2.277336"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1482)
                     /locus_tag="PLC71_09955"
     CDS             complement(<1..>1482)
                     /locus_tag="PLC71_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03000.1"
                     /translation="KRRLSKALAMFLAAALTIPAAVPVAASESTADKEETVYVKTDAN
                     GEEKTVIVSDWLKNFSGEAKIKDATDLTDVENVKGNETYKRGKKDSITWNANGKDIYY
                     QGTSDKNLPVSMKVTYYLDGKEISPKKLVGKSGKVKIRYEYENHSAVTKTINGKQTTV
                     YTPFTIITAAILNTDNFSNVKATNGKVISDGNKHIVIGVAMPGLADSLNLKNTSIGSN
                     FDIPEDFEITADVEDFQMTVTATVANSDTLSEFGLDDAGDLSELTDSLKDLENATDKL
                     CDGSGDLVKGIEKLVTASGKLKSGTKQIAVSSKQLATGLDKLVNGSTTLKNGTSQLAK
                     GTSALPSSTEKLDNGVRQILKELQNSTPSESDQAKLQSNLQSAQTGITKELMNIKTQT
                     ESVGVSAKTLGALAESIGASATDVGDQAGAIGEFIKNNYSSLTDEQKAQLTTIAGSLK
                     TDAGNLGGYAQSVGGQAKNLGTYAGSIGTSLQETQTDLQIVSG"
     assembly_gap    1483..1492
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1509..>2698)
                     /locus_tag="PLC71_09960"
     CDS             complement(1509..>2698)
                     /locus_tag="PLC71_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRM03001.1"
                     /translation="FALGANLGIVMAKGVIIGVICCVTVLPSLILICDKLIEKTHHRS
                     LMPDLTNASHFITKHYKTTIVVFLILLFPAIYGNNHTEIYYNIDKSLPQSLPSNVANK
                     KLSDEFGMSNIHMVMIKNGMSQKELSNMQKEIDKVDGVEWCLGMNSVIGSSIPSDMIP
                     SSMKEMLQSDEYEMQFICSNYKSATEKVNKQINEINNIVKKYSPGSMVIGEAPLMNDL
                     ETVTNVDLRNLNIASIAAIFVIILIVFKSISLPVILVAVIEFAICVNMAVPYYTGLSL
                     PFVASIVIGTIQLGATVDYAILMTSRYQKERINGKKKMEAIRIAHETSMQSIITSGLS
                     FFAATIGIAIYSNIDMISAICTLLARGAVISTVAVIFILPAMFMIFDKLICKTTLKMG
                     HLE"
BASE COUNT          693 a          578 c          463 g          954 t
ORIGIN      
        1 catcctgata cgatctgaag gtctgtctgt gtttcttgca agcttgttcc gatagatcct
       61 gcgtaggttc ctaaattctt tgcctgacct cctacggatt gtgcataacc gcctaagttt
      121 cctgcatctg tctttaaaga acctgcaatc gttgtaagct gtgctttctg ctcatctgta
      181 agagaactat aattattctt gataaattct ccaattgctc ctgcctgatc tccaacatct
      241 gttgctgatg ctccaataga ctctgctaat gcacctaatg tcttcgctga cacaccaaca
      301 ctttctgtct gtgtcttaat attcattaat tcttttgtga ttcctgtctg tgcactctgt
      361 aagttagact gtaattttgc ctgatcactt tcgcttggtg tactattctg cagttctttg
      421 agaatctgtc tcactccatt atctaacttc tctgtagatg atggtaaagc actggttcct
      481 ttcgctaact gactggttcc attctttaat gttgtagatc cattgaccag tttgtcaagt
      541 cctgttgcta actgtttgga actgactgca atctgttttg ttcctgattt taattttcca
      601 gaggctgtga ctaatttttc aattcccttt actaaatctc cagaaccatc acataactta
      661 tctgtcgcat tttcaaggtc ctttaaggaa tctgttaatt cacttaaatc tcctgcatca
      721 tctaaaccga actctgataa tgtatcggaa ttcgcaactg tcgctgtgac tgtcatctgg
      781 aagtcttcta catcagctgt gatctcgaaa tcttctggaa tatcgaagtt tgacccaata
      841 cttgtattct taaggtttaa gctgtctgct aatcctggca ttgcaactcc aataacgata
      901 tgtttgttac catcggagat aacttttccg tttgttgctt tgacattaga gaagttatct
      961 gtatttaaga tcgctgctgt gattattgta aatggtgtat agactgttgt ctgttttcca
     1021 ttgatcgttt tagttacggc ggaatgattt tcatattcat aacgaatctt tacttttcct
     1081 gatttaccaa ccagtttctt tggtgaaatt tcttttccat ccagatagta tgtgactttc
     1141 atggatactg gcagattttt gtcacttgtt ccctgatagt aaatatcttt tccattggca
     1201 ttccatgtaa tactgtcctt ttttcctcgt ttatatgtct cattcccttt aacattctca
     1261 acgtctgtca ggtctgtagc atctttgatc tttgcttcac ctgagaaatt ctttaaccag
     1321 tcactgacaa taactgtttt ctcttctcca ttcgcatccg tttttacgta tacggtttct
     1381 tctttatctg cagtggactc gctggctgct accggtactg ctgccggaat tgtaagagct
     1441 gcagcgagaa acattgctaa tgctttcgat aatcttcttt ttnnnnnnnn nncataacaa
     1501 tcgctctcct attctaaatg acccatcttg agggttgttt tacagatcaa tttatcaaat
     1561 atcataaaca ttgccggcag aataaagatt acggcaacgg tactgatcac agctcctcgt
     1621 gcaagtaagg tacagatcgc actgatcata tcaatatttg agtaaatcgc aataccgatc
     1681 gttgctgcga agaaacttaa tccacttgta atgatagact gcatggatgt ttcatgtgca
     1741 atacggatcg cttccatttt cttcttgcca tttatacgct ctttctgata acgacttgtc
     1801 atcaggatcg cgtagtcaac ggttgcacct aactgaatcg ttccgataac gatacttgca
     1861 acaaacggca gtgataatcc cgtgtaatat ggaactgcca tattgacaca gattgcaaat
     1921 tcgatcacgg ctaccaggat caccggtaag gaaatggatt taaatacaat taaaatgatc
     1981 acaaagattg ccgcgatcga cgcgatattt agatttctaa ggtcaacatt ggttactgtc
     2041 tccaagtcat tcatcaatgg tgcttctcca atgaccatag aacctggact gtatttcttc
     2101 acaatgttgt tgatctcgtt gatctgttta tttactttct cggttgctga tttgtaattt
     2161 gaacagataa actgcatctc atactcatca ctttgcaaca tttctttcat ggaagaagga
     2221 atcatatcgg atggaatact agaaccgatc acggagttca ttccgagaca ccattcaact
     2281 ccgtcgactt tatcgatctc tttctgcatg ttgcttaatt ccttctgact catgccgttc
     2341 ttgatcatga ccatatggat gttactcatt ccaaattcgt cacttaattt cttattcgcc
     2401 acattactcg gtaagctttg tggaagtgat ttgtcaatat tatagtaaat ctctgtatga
     2461 ttatttccat aaattgctgg gaacagcaaa attaaaaata ctacaatcgt tgttttataa
     2521 tgttttgtga taaagtgtga tgcattcgta agatctggca tcagtgaacg atggtgtgtc
     2581 ttctcaatta atttatcaca gatcaggatc aatgatggca aaactgttac acaacagata
     2641 accccgataa taacaccttt cgccataacg atacctaagt tagctcctaa tgcaaatg
//
LOCUS       DAOMUE010000283         2690 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10874_length_2690_cov_2.752941, whole genome shotgun sequence.
ACCESSION   DAOMUE010000283 DAOMUE010000000
VERSION     DAOMUE010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10874_length_2690_cov_2.752941"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLC71_09965"
     CDS             complement(<1..262)
                     /locus_tag="PLC71_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRM03002.1"
                     /translation="MIKVFLVEDEKIIRKSIKNNVKWEENGFEFAGEAPDGEMALPMI
                     EKLHPDIVITDIKMPFMDGLELSDILKKKMPKIQIIILSGYGE"
     gene            complement(405..1421)
                     /locus_tag="PLC71_09970"
     CDS             complement(405..1421)
                     /locus_tag="PLC71_09970"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRM03003.1"
                     /translation="MMKVKMIEKRQNGVCLFELQNDEIKVITSNLGCHILSVFTKDRD
                     GNYGDVVLGYQDVEDCHKDDKFLGCIIGRVANRIANGEFQLNGKTYKLAVNSGPNTLH
                     GGINGFNQKIFNYEILEDGIRFIYLSPDMEEGFPGSLYLKIVYRLSGNELKMEYEAMT
                     DQDTLINIANHSYFNLSAKDSKIYDHQLMIKSDQIACADENGLPTGKFLDVENTPFDF
                     KSFHEIGERIHDDDEQLRFVGGYDHCFMLKDEKDHAVLYDKESGRKLTITTTLPCMQL
                     YAGNFLAGGSDGKFGKPYENRDGVALEPQFLPNSMNIEKKPRVILRKGEEYEAVTTYR
                     FETE"
     gene            complement(1475..2167)
                     /locus_tag="PLC71_09975"
     CDS             complement(1475..2167)
                     /locus_tag="PLC71_09975"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HRM03004.1"
                     /translation="MLEQLKKEVYEANMELPKRGLITYTWGNVSGIDRESGLFVIKPS
                     GVDYDKLSPEDMVVMDLDGNRVEGDLNPSSDTPTHMELYKAFKDLGGIVHTHSPWATS
                     WAQAGRSIPCYGTTHADYFYGEIPCARSLTKEEIEEAYEKNTGLVIIETFKDKNPVYV
                     PGVLCTNHGPFTWGADAAEAVHNAVVLEEVAKMAYRCEHLNPEAKPAPQYLQDKHFFR
                     KHGANAYYGQGK"
     gene            complement(2182..>2690)
                     /locus_tag="PLC71_09980"
     CDS             complement(2182..>2690)
                     /locus_tag="PLC71_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRM03005.1"
                     /translation="LLAYNYFSGEHITGFEEGRPLFVRKPDSNFNLANFMRVHLYTAL
                     GALKTGLDILMKEEDVKVDKMLGHGGLFKTEGVGQKIMAAAVNAPVSVMKTAGEGGAW
                     GMAILAAYMLDKAEDETLDTYLSDKVFAGEEGTTIAPDEKDVEGFDEFIKQYQKGLAL
                     ERVAIDVM"
BASE COUNT          741 a          589 c          364 g          996 t
ORIGIN      
        1 actccccata tccagaaagt atgatgatct gtattttggg catttttttc tttaagatat
       61 cacttaattc aagtccatcc ataaatggca tcttgatatc tgtgatcacg atatctggat
      121 gtagtttttc gatcattgga agagccatct caccatctgg tgcttctccc gcaaattcaa
      181 aaccattttc ttcccatttt acgttattct ttatactctt acgaatgatc ttttcatctt
      241 ctactaaaaa tactttgatc atattacctt cctcttttct aaaatattat tatactctct
      301 tatagaatta tattccagaa ataaacaaga atatatccat acaaataaaa aggggaaggg
      361 gatattgtat ggatatgatt cttatttttt aatttcaatc tatattattc tgtttcaaat
      421 cggtatgttg tcactgcttc atactcttcg ccttttctta agataactct tggctttttc
      481 tctatattca tagaattagg caagaattgt ggttctaatg caacaccatc tctgttctca
      541 tatggttttc caaatttacc atcacttcct cctgccagaa agtttcctgc atataactgc
      601 atgcatggaa gtgttgttgt gatcgttaat tttcgtccac tttctttatc atataagact
      661 gcatgatctt tctcatcctt taacataaaa caatgatcat agcctccaac gaatctcaac
      721 tgttcatcat catcatgaat ccgttctcca atctcatgaa aacttttaaa atcaaatggt
      781 gtattctcta catccaaaaa cttacctgtt ggaagaccat tctcatctgc acatgcaatc
      841 tgatctgatt tgatcatcaa ttgatgatca tagatcttac tgtcttttgc tgaaagatta
      901 aaataggaat gattggctat attgatcagt gtgtcttgat ctgtcattgc ttcatattcc
      961 atctttaatt catttccaga tagacgatat actatcttta aataaagact tcctggaaat
     1021 ccttcttcca tatctggaga aagatagata aaacggattc catcttctaa aatctcatag
     1081 ttaaaaatct tctgattaaa tccatttata ccaccatgaa gcgtattcgg tccggaattt
     1141 actgcaagtt tataggtctt tccattcaac tgaaactctc catttgcaat tctgtttgca
     1201 acacgtccga tgatacatcc taaaaattta tcatctttat gacaatcttc cacatcttga
     1261 tatccaagaa cgacatctcc ataattccca tctctgtctt ttgtgaacac agataaaata
     1321 tgacaaccaa ggttactcgt gatcactttt atctcatcat tttgtaattc aaataagcag
     1381 actccatttt gtcttttttc gatcattttt actttcatca ttttacaaca ctttctttta
     1441 aattttatct gatatcttta ttgatccatt ttcattattt gccttggcca tagtaagcat
     1501 ttgccccatg ttttctgaag aaatgtttat cctgcagata ttgtggtgct ggctttgctt
     1561 ctggatttaa atgctcgcaa cggtatgcca tctttgcgac ttcttctaag acaacggcat
     1621 tatgaactgc ctcggctgca tcagctcccc atgtaaatgg tccatgattt gtacaaagca
     1681 ctcctggaac ataaactgga ttcttatctt taaatgtctc aatgatcacc aatcctgtat
     1741 tcttctcata ggcttcttca atttcttctt ttgtgaggct tcttgcacat ggaatttctc
     1801 catagaagta atctgcatgt gttgttccat agcatggaat acttctcccc gcctgtgccc
     1861 atgatgttgc ccatggggaa tgtgtatgta caattcctcc taaatctttg aatgctttat
     1921 ataattccat atgcgttggt gtatcagaag atggatttaa atctccttcc actctgtttc
     1981 catcaagatc catgacaacc atatcttctg gagaaagttt atcataatcc acaccacttg
     2041 gtttaataac aaataaacca ctttctctat caattccact gacatttccc catgtatatg
     2101 tgatcagacc tcttttagga agttccatat ttgcttcata gacttctttt tttaattgtt
     2161 ctaacatttt tcgtctcctt tttacatgac atcaattgca actcgttcaa gtgcaagacc
     2221 tttttgatat tgtttgataa attcatcaaa tccttccaca tctttttcat ctggagcgat
     2281 cgttgtacct tcttcccctg caaatacttt gtcagaaaga taggtatcaa gcgtctcatc
     2341 ttctgcttta tccagcatat aggctgcaag gatcgccatt ccccatgcac caccttctcc
     2401 agctgttttc ataactgata ctggtgcatt cacagctgct gccatgatct tttgtccaac
     2461 accttctgtt ttgaataatc caccatgtcc cagcatttta tcaactttga catcttcttc
     2521 tttcatcaag atatcaagcc ccgtcttcaa agctccaagt gctgtgtata gatgaactct
     2581 cataaagttt gcaagattaa aattactatc tggttttctt acaaataatg gccttccttc
     2641 ttcaaatcct gtgatatgtt ctcctgaaaa atagttgtat gcaagtaatc
//
LOCUS       DAOMUE010000284         2682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10915_length_2682_cov_3.214313, whole genome shotgun sequence.
ACCESSION   DAOMUE010000284 DAOMUE010000000
VERSION     DAOMUE010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10915_length_2682_cov_3.214313"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1159)
                     /locus_tag="PLC71_09985"
     CDS             complement(62..1159)
                     /locus_tag="PLC71_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HRM03006.1"
                     /translation="MSYYSVNLPSYTIGEGCYKEIPHTTRFYGKTAVVIGGKIAMSKA
                     KDKILEGIKDSDVEILDFIWYGGDSTYENGNALIDMDVVKKADMIFAVGGGRACDTCK
                     YVANEMDKPLFCFPTVASNCASVTAISVIYNPDGSFREYYYPKNANHTFIESSIIADS
                     PEELLWAGIGDALSKECEAVFASKEDELSHTPMMGVQLSKICTTPLVEYGKKALDDLR
                     ANKVSYELEQVALDIIISTGLVSNMVSAAPKYYYNSSLAHCVYYGSTVTKGGEKHLHG
                     EIVSLGVLCLLTFGKEYEERERIAKFNLSMGLPVCFEDIEITEDDFDAMAEKAMTTTE
                     WDFKPENAGVTKESFIQCMKETDEYGRSIKF"
     gene            complement(1274..1783)
                     /locus_tag="PLC71_09990"
     CDS             complement(1274..1783)
                     /locus_tag="PLC71_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HRM03007.1"
                     /translation="MNKMNENRKIVLDTLNEQNIEYEMFEHQAVYTVDEIEDSDLWNH
                     ENGIGAKNLFLRDGSGKHHFLVIIREDKQADLKKVRDEIGSSRLSFASAERLMKYLNI
                     EPGSVSPLGVLNDQECVIPVFFDEDLKEYEYIAVHPNDNTATIWMKLADLVNVIKLHG
                     NQVKFINVE"
     gene            complement(1783..2676)
                     /locus_tag="PLC71_09995"
     CDS             complement(1783..2676)
                     /locus_tag="PLC71_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRM03008.1"
                     /translation="MTKIKLTDNLSLSQIIRGFWRLDTWELSTDELIDNMKSSIDLGV
                     TSFDTAEIYADTLCESQIGEAFAKDPALRGKVELISKTGIYKQEINGESFGYYNTTYD
                     RIVRSCKESLQRLHTDYLDLYLIHREDPLLDPWEAGRAMLDLKKEGLIKEIGVSNFDP
                     FKFDAMNKAVDGALVTNQIEWNPVCFEHFNSGMMDYLTVNKIHPMIWSPLAGGRLFQE
                     DDLQCKKAMDKIKEIAERHNCEPDTIVYAWIMYHPVQAMPIVGSRKVERIESAVKAMD
                     VKLEHYEWYEIYAASGQQQIR"
BASE COUNT          703 a          586 c          404 g          989 t
ORIGIN      
        1 tttcggttgc ttatttattt tttatcatat actcgaaatc tacagttggc caacttacaa
       61 attaaaattt tatacttcta ccatattcat ctgtttcttt catacactga ataaagcttt
      121 ccttcgtaac cccagcattc tcaggtttga agtcccattc tgtagtggtc attgctttct
      181 ctgccattgc atcaaaatca tcttctgtga tctcaatgtc ttcaaagcat actggcagtc
      241 ccatacttag gttgaatttt gcgattcgtt ctctttcttc gtattctttt ccgaatgtta
      301 acaggcatag cacacctaat gatacgattt ctccatgtaa atgtttttct ccgccttttg
      361 taacggttga tccgtaatat acacagtgtg caaggcttga gttatagtaa tattttggtg
      421 ctgcacttac catgttagat actaatcctg tactgatgat gatgtctaac gctacctgtt
      481 ctaattcata agatacttta ttcgctctta agtcgtccaa cgctttcttt ccatattcaa
      541 caagtggtgt agtacagatc ttgcttagct gtactcccat cattggtgta tgggataatt
      601 cgtcttcttt ggatgcaaat actgcttcac attctttgct taatgcatct ccgattcctg
      661 cccataataa ttcttctggt gaatcagcga tgatggatga ttcgatgaat gtatggtttg
      721 cattctttgg atagtaatat tctctgaatg atccatctgg gttataaatt acagagattg
      781 ctgtgacaga tgcacagttt gatgctacgg ttgggaagca gaataatggt ttatccattt
      841 catttgccac atatttacat gtatcacagg cacgtcctcc accgactgcg aagatcatgt
      901 ctgctttctt gacaacgtcc atgtcgatca atgcgttacc attttcatag gtggaatcac
      961 caccgtacca gataaaatca aggatttcta catcggaatc tttgattcct tctaagatct
     1021 tatcttttgc tttagacatc gcgatctttc caccgatcac aacagctgtt tttccataaa
     1081 atcttgttgt atgcggaatc tctttataac atccctctcc gatcgtataa cttggtaaat
     1141 tcacactata ataactcatc ttgttcgttg ccttctttct ctttattctt ttgactcttt
     1201 atattggcta cattatagtc ctcaattctc aaaaaatcaa agaatcttgt gttctttttc
     1261 ttattcatga attttattcc acattgataa atttcacttg atttccatgc aactttatga
     1321 cattcacaag atcagcaagt ttcatccaga tcgttgctgt attatcattt ggatggactg
     1381 caatgtattc atattctttc agatcttcgt caaagaatac cggaatcaca cattcctgat
     1441 cgttcaaaac gcctaacggc gaaacagatc cgggttcaat atttaaatat ttcattagac
     1501 gctcggctga ggcaaaactt agtctggaac ttccaatctc atctcttact ttcttaaggt
     1561 ccgcctgctt gtcttcccga atgatcacaa gaaaatgatg ctttccgctg ccatctcgta
     1621 aaaataaatt cttagcccct attccattct catgattcca caggtcagag tcttcgattt
     1681 catcgactgt gtataccgct tgatgttcaa acatttcata ttcgatgttc tgctcattta
     1741 aagtatctaa cacgattttt ctattttcat tcattttatt cattatctta tctgctgctg
     1801 tccgcttgct gcataaatct cgtaccattc ataatgctct aatttaacat ccattgcttt
     1861 cactgcagat tcaatcctct caacctttct gcttccaacg atcggcattg cctgtactgg
     1921 atgatacatg atccatgcat acacgatcgt atctggttca cagttatgtc tctcagcgat
     1981 ttctttgatc ttatccattg ctttcttaca ttgtagatca tcttcttgaa ataatcttcc
     2041 tcctgcaagt ggtgaccata tcattggatg aattttatta actgtcagat aatccatcat
     2101 tccagaattg aaatgttcaa aacatactgg gttccattca atctgattcg ttacaagtgc
     2161 tccgtcaacc gctttattca ttgcatcaaa tttaaatgga tcaaaatttg acactccgat
     2221 ttctttgatc agcccttctt tcttcagatc cagcattgct ctgcctgcct cccatggatc
     2281 taaaagaggg tcttctctgt gaattaaata taggtcaaga taatctgtat gtaatctctg
     2341 aagactttct ttacaggatc gtacgattct atcataagtt gtattataat acccaaatga
     2401 ttctccatta atttcttgtt tgtaaattcc tgtttttgaa atcagctcta cttttcccct
     2461 taatgccgga tctttggcaa atgcttcacc tatctgagat tcacataaag tatctgcata
     2521 aatctctgct gtatcaaaag atgttacacc taagtctatg cttgatttca tgttatcaat
     2581 aagttcgtct gttgataatt cccaagtatc cagtctccaa aaacctcgga tgatctgaga
     2641 taaggaaagg ttatctgtta atttaatctt tgtcatatta at
//
LOCUS       DAOMUE010000285         2679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_10932_length_2679_cov_2.289253, whole genome shotgun sequence.
ACCESSION   DAOMUE010000285 DAOMUE010000000
VERSION     DAOMUE010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10932_length_2679_cov_2.289253"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLC71_10000"
     CDS             complement(<1..522)
                     /locus_tag="PLC71_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRM03009.1"
                     /translation="MPRRKPSSVKIIPLGGLGQIGMNITAFECNNQILIVDCGLAFPD
                     EDMLGIDLVIPDITYLKDNASKIRGLVITHGHEDHIGAIPYFEKELNVPIYATKLTMA
                     LIENKLKEHGILNNVERHVVTHGDKVKLGIFNVEFIKTNHSIADAAALAITTPAGVIV
                     HTGDFKIDHTPLFG"
     gene            complement(698..973)
                     /locus_tag="PLC71_10005"
     CDS             complement(698..973)
                     /locus_tag="PLC71_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HRM03010.1"
                     /translation="MSKKDSKILFLDDDGQEIEFNVIEQTTIAGQTYLLVEDCEASSE
                     DESVVLIMKEVQLEDDYVSYEIVEDDKELDMISDIFNALLDGLEVSL"
     gene            complement(986..1405)
                     /gene="ruvX"
                     /locus_tag="PLC71_10010"
     CDS             complement(986..1405)
                     /gene="ruvX"
                     /locus_tag="PLC71_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRM03011.1"
                     /translation="MRRILGLDYGTKTVGVALSDELHITAQPLETITRKDANKLRKTY
                     ARIEELVKEYDVEKIVLGLPKNMNNTVGERGEDTLSFRDALVRRTGLEVILWDERLTT
                     VASEKVLMESGVRRENRKKVIDQIAASMILQGYLDSL"
     gene            complement(1419..1673)
                     /locus_tag="PLC71_10015"
     CDS             complement(1419..1673)
                     /locus_tag="PLC71_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HRM03012.1"
                     /translation="MDNNQTQHFSVVREELDVHMILERVYEALQEKGYNPVNQMVGYI
                     MSGDPTYITSHNNARSIINKVERDDIIEALLENYIETRLK"
     gene            complement(1758..>2679)
                     /locus_tag="PLC71_10020"
     CDS             complement(1758..>2679)
                     /locus_tag="PLC71_10020"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRM03013.1"
                     /translation="DQEYESLHVEQLKEHTRAYIKVQDGCNQFCSYCIIPYARGRVRS
                     RKMEEVLEEITNLTKHGCQEFVITGIHVTSYGKDLGDVTLIDLLEEIAKIPEVKRIRL
                     GSLEPGFITKDTLDRLSKMESFCPHFHLSLQSGCDSVLKRMNRKYTTQDIREKCKAIR
                     EHFQYPALTTDIIVGFPGETEEEFEETRKFLEEIDLYEMHVFKYSIRKGTKAADMKPQ
                     INDQIKAKRSNVLLEMSKQHQKEFETKQLGMVKEVLIEEKMHGKDHYYTGHTKEYIKV
                     ALYSEEPLENKIVKVKLRQILDDGYVLAEC"
BASE COUNT          682 a          626 c          402 g          969 t
ORIGIN      
        1 cccaaataat ggcgtatggt cgatcttaaa gtcacctgta tgcacgatca cacctgctgg
       61 tgttgtgatc gcaagtgctg ctgcatctgc aatactgtga tttgtcttaa taaattctac
      121 attaaagatt ccaagtttta ctttgtcacc atgtgtcaca acatggcgct ccacgttatt
      181 taaaataccg tgctctttta atttgttctc gatcaatgcc atggtaagtt ttgtcgcata
      241 gatcggtacg ttcaattcct tctcgaaata aggaatcgct ccaatatggt cctcatgacc
      301 atgagtgata accagtccac gaatcttgct tgcattgtcc tttaaatatg tgatatctgg
      361 aataactaaa tcaattccta acatatcctc atctgggaac gcaagtccac agtccacgat
      421 caaaatctgg ttattgcatt caaatgctgt gatgttcatt ccgatctggc cgagtccgcc
      481 aagtggaatg atctttactg agctaggctt tcttctaggc attggtcttc ttccccttgc
      541 ctgataatta ttcttattgt atgatttctt gttatcattt gcataataat tatttcgttt
      601 tggtcttgcc tgctgtctta cttgattgtc tttcaacttt cttcctccat atctacgtag
      661 gtctgacaat ccattgtttt aaacactctt atatatttta taaactcact tcaagtccat
      721 ctaacagtgc gttaaaaata tctgagatca tatccagttc tttatcgtcc tcaacgatct
      781 cataggatac atagtcatcc tctaactgca cttctttcat gatcaataca acgctctcat
      841 cttcactcga tgcttcgcag tcttctacca aaaggtaagt ctgacctgcg atcgttgtct
      901 gttctatgac attaaattcg atttcctgtc catcgtcatc cagaaaaaga atcttgctgt
      961 ctttcttgct cattgtctgt tcctcctata gggaatctaa atatccctgt aaaatcatag
     1021 acgccgcgat ctggtcgatc actttctttc tgttttcgcg tcttacaccg ctttccatca
     1081 gaactttctc tgatgctact gttgtcagtc gctcatccca caagatcact tctaatcctg
     1141 tacgacgcac taaggcatcg cggaaagata aagtatcttc tccgcgctca cctactgtat
     1201 tattcatatt ctttgggaga cctaaaacaa tcttctcaac atcatattct ttcacaagtt
     1261 cttcgatcct tgcgtaagtc tttcttaact tattcgcatc tttccttgtg atcgtctcta
     1321 atggctgtgc tgtaatatga agctcatcac ttaacgccac tccgactgtc ttcgtcccat
     1381 aatctaaccc taatatcctt ctcataatac cacggtctct actttaatct tgtctcaata
     1441 tagttctcaa gcagtgcttc aatgatatcg tcacgctcga ctttattgat gatgcttctt
     1501 gcattgttgt gactggtaat gtatgttgga tctccagaca taatatatcc taccatctga
     1561 ttgacagggt tatatccttt ctcctgcagt gcctcatata cacgctctaa gatcatgtgt
     1621 acatccagtt cctctctaac aacggaaaaa tgttgtgttt gattattatc cataattatc
     1681 acgtcccttt catttattaa ccattttaat ataaaatgta tgaaatagca atggaaatca
     1741 taaaatttca catctgttta acactctgcc aatacatatc catcatctaa gatctgtctt
     1801 agctttactt tcacgatctt attttccaat ggttcttcac tgtaaagtgc aacttttata
     1861 tactcctttg tatgccctgt ataataatga tcttttccat gcatcttttc ttcgatcagt
     1921 acttctttga ccatccctaa ctgttttgtc tcaaattcct tctggtgttg ttttgacatt
     1981 tctaataaga cattacttcg ttttgcctta atctgatcat tgatctgtgg cttcatatct
     2041 gctgccttcg ttccctttcg aatggaatat ttgaacacat gcatttcata gagatcgatc
     2101 tcttctaaaa attttcttgt ctcttcaaat tcttcttctg tctctccagg aaaacctacg
     2161 ataatatctg ttgtcaatgc cggatattgg aaatgttcac gaattgcctt acatttctct
     2221 ctgatatctt gtgttgtata cttacggttc attcgtttta agacagaatc acatccactc
     2281 tgcagggaca gatggaaatg tgggcaaaaa ctttccatct tagaaaggcg gtccaatgta
     2341 tcctttgtga taaatccagg ttccagagat cccaaacgga ttctcttaac ttccgggatc
     2401 ttggcaatct cttctaacag atcgatcagt gtaacatctc ccaaatcttt tccatacgat
     2461 gtaacatgaa ttcctgtgat cacaaattcc tgacatccat gttttgtcag attcgtgatc
     2521 tcttctaaca cttcttccat cttacggctt ctgactcttc ctcttgcata agggataatg
     2581 cagtaagaac aaaactgatt gcatccatct tgtactttaa tatatgctct ggtatgttct
     2641 tttaattgtt ccacatgcaa agattcatat tcctgatca
//
LOCUS       DAOMUE010000286         2639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_11145_length_2639_cov_2.785217, whole genome shotgun sequence.
ACCESSION   DAOMUE010000286 DAOMUE010000000
VERSION     DAOMUE010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11145_length_2639_cov_2.785217"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLC71_10025"
     CDS             <1..184
                     /locus_tag="PLC71_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03014.1"
                     /translation="SLGNQGFASLRENHCFYIDKTDFIREWWEEQDVVTLIKYEKRYQ
                     TSSESFGIFFRVITFW"
     gene            complement(168..944)
                     /locus_tag="PLC71_10030"
     CDS             complement(168..944)
                     /locus_tag="PLC71_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HRM03015.1"
                     /translation="MFEKIKCFILDMDGTIYLGNELFPFTKDFLKKVEETGREYYFFT
                     NNSSKSQRAYIEKLGKLGIPIKKDQMMISSHVIIKYLKEHYDGKSIYVLGTPSLIQEF
                     QYFDMNLTDEDPDIVVLGFDTTLTYEKLSKACHYIRNGCTYFGINPDWNCPIEGGAFI
                     PDCGSMAKLIEASTGRFPEFFGKPSKHTLDYIIQETGYEPDEIAIVGDRLYTDIAVAD
                     QSDVTSILVLSGESTREDVKTSDVKPNVILEDLSEITKML"
     gene            complement(956..2245)
                     /locus_tag="PLC71_10035"
     CDS             complement(956..2245)
                     /locus_tag="PLC71_10035"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HRM03016.1"
                     /translation="MKINIKEFTGKCSCGRDHQLVVDDVILEEGALKKLPEILNKEPY
                     NQYKHLVMVCDDNTYEAAGKEVEKLLNGISVIKLDPENLHANEIGVAKVKEQLDPIKE
                     VDCMIAVGSGTVHDLTRYNAYERKIPFISVPTAASVDGYVSTVAAMSWYGFKKSMIAE
                     SPILVVADSRIITDAPMRLTASGVGDLLGKYTALADWKITNILDGEYICDRICEMEYD
                     ALDKLKESLDGLSNRDINAYEELMYGLLLSGLAMQMTGHSRPASGAEHHMAHFWEMAV
                     INDEIDAYHGEKVGVGLIQVSDIYHEAVKYLKEGNFKVKDHVDIETDLIEKTFTNKEL
                     CESIKKTNDPNLLDLIDPKVLREKTEEIIRIIEEIPKADEIVAMLEKVHGVKSLEDIG
                     FTPDMKEKTARVAPYIRDRITFMRILKFYDFYDDVIK"
     assembly_gap    2334..2343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2344..>2639)
                     /locus_tag="PLC71_10040"
     CDS             complement(<2344..>2639)
                     /locus_tag="PLC71_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM03017.1"
                     /translation="EKKGCVGSDYGKKAVENVKEYSDYIIISKGIALAHAGKKEAHVY
                     KDGLSLVMCPEGIEFTEGNIVYLVFCFAVAEEKDYLKLFQEIIALGKTQKKMKD"
BASE COUNT          686 a          560 c          414 g          969 t
ORIGIN      
        1 ttctctcggc aatcaaggtt ttgcttctct tcgtgaaaat cattgttttt atatcgataa
       61 aacagatttt atcagagaat ggtgggaaga acaggatgtt gtaactttga tcaaatatga
      121 aaaaagatac caaactagta gtgaatcatt tggtatcttt tttcgcgtta taacattttg
      181 gtgatctcgg agagatcttc taagatcaca ttcggtttta catcgcttgt ttttacatct
      241 tctcttgtgc tctctccact tagcactaag attgatgtca catcactttg atctgcaact
      301 gcgatgtcgg tatacagccg gtcccctaca atagcgatct cgtccggttc gtatcccgtt
      361 tcctggatga tatagtccag tgtatgtttc gacggttttc caaagaattc tggaaatctt
      421 cccgttgaag cctcaattaa ttttgccatg gaaccacagt ctggaataaa tgctccgcct
      481 tctatcgggc agttccagtc tggattgatt ccaaagtatg tacagccatt tcggatataa
      541 tgacatgctt tggaaagctt ttcataagtt aaggttgtat cgaatcctaa gactacgata
      601 tctggatctt cgtctgtcag gttcatatca aaatattgaa attcctggat cagggatggg
      661 gtccctagca cataaatact ctttccatcg taatgttctt taagatattt gatgatcaca
      721 tgacttgaga tcatcatctg atctttcttg atcggtatcc caagcttccc aagtttctca
      781 atataagccc gctggctttt tgatgaatta ttggtaaaga aataatattc tcttccagtt
      841 tcctcgactt tctttaaaaa atctttggtg aatgggaata gttcattccc aagatagatc
      901 gttccatcca tatcaaggat aaaacatttg attttttcaa acatgagctg ccctcctatt
      961 tgatcacatc atcataaaaa tcatagaatt tcagaattct cataaatgtg atacggtcac
     1021 ggatatatgg tgcaactctt gctgttttct ctttcatatc cggtgtaaat ccgatgtctt
     1081 ctaagctttt tactccatgt actttctcta acattgcaac aatctcatct gcttttggaa
     1141 tctcttctat gatcctgatg atctcttctg ttttttctct taatactttt ggatcgatca
     1201 gatctaacag atttggatca tttgtctttt taatgctttc gcataattct ttattggtaa
     1261 atgtcttttc gatcaggtct gtctcaatat ccacatgatc ttttaccttg aagttgccct
     1321 cttttaaata ttttactgct tcatggtaga tgtcacttac ctggatcagt ccgactccta
     1381 ctttctctcc atggtatgca tcgatctcat cattgatcac ggccatctcc cagaaatgtg
     1441 ccatatgatg ctctgctccg gatgcgggac gggaatgtcc tgtcatctgc atggcaagtc
     1501 ctgataataa taatccatac atcaattctt catatgcgtt gatatctctg ttacttaatc
     1561 catctaagga ttcttttaat ttatccagtg catcatattc catctcacag atacggtcac
     1621 agatatattc tccatccaga atatttgtga tcttccagtc tgcaagggcc gtgtattttc
     1681 ctaacagatc tccgactccg gatgctgtca gtctcattgg tgcatctgta atgatacggc
     1741 tgtctgcaac gactaaaata ggcgattctg caatcatgct cttcttaaat ccataccatg
     1801 acatagctgc aactgtagat acataaccat ctacactggc tgcggttgga actgagataa
     1861 atggaatctt tctttcatat gcattatatc ttgtcagatc atgaactgtt cctgatccga
     1921 ctgcgatcat acagtctact tctttaatcg gatctaattg ttctttgact tttgctactc
     1981 cgatctcgtt tgcatgaaga ttttctgggt ctaatttgat cacagaaatt ccatttaata
     2041 atttttctac ttctttccct gctgcttcgt aggtattgtc atcacatacc ataacaagat
     2101 gtttatactg attgtatggt tctttgttta agatttctgg aagttttttt aatgctcctt
     2161 cttctaagat tacgtcatct acaactaatt ggtggtctct gccacaactg cattttcctg
     2221 tgaattcttt gatattgatt ttcatgtcaa acaccctttc ctgtctatct tttataaaaa
     2281 tacattttca tttaaaatac caatgaatgg tataaagaaa caacattttt ctgnnnnnnn
     2341 nnntatcttt catctttttt tgtgttttgc ctaacgcaat aatttcctgg aataacttca
     2401 gataatcttt ttcttccgca actgcaaagc aaaataccaa atatacgata tttccttccg
     2461 taaattcgat tccttctggg cacattacaa gacttaatcc atctttgtag acatgtgcct
     2521 ctttctttcc tgcatgcgct aaagcaattc ctttactgat aataatataa tcactatatt
     2581 cttttacatt ttctactgct ttttttccgt aatcagaacc aacacagccc tttttctct
//
LOCUS       DAOMUE010000287         2622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_11242_length_2622_cov_2.538372, whole genome shotgun sequence.
ACCESSION   DAOMUE010000287 DAOMUE010000000
VERSION     DAOMUE010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11242_length_2622_cov_2.538372"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1346)
                     /gene="purD"
                     /locus_tag="PLC71_10045"
     CDS             complement(75..1346)
                     /gene="purD"
                     /locus_tag="PLC71_10045"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRM03018.1"
                     /translation="MKVLIVGSGGREHAIAWSVAKSPKVDKIYCAPGNAGIAEFAECV
                     NIKAMEFDKLVAFAKENAIDLTIIGMDDPLVGGVVDAFESEGLRVFGPRKNAAIIEGS
                     KAFSKDLMKKYKIPTAAYENFTDPDEAIKYLETAKMPIVLKADGLALGKGVLICNTLE
                     EAKEGVKTLMLDKQFGDAGNEIVIEEFMTGREVSVLAFCDGKTIKCMTSAQDHKRAKD
                     GDQGLNTGGMGTFSPSPFYNDEVEAFCEKYVYQSTIDAMASEGRPFTGILFTGLMITE
                     DGPKVLEYNARFGDPEAQVVLPRMKNDIIDVMEACIDGKLSDVELEFEDNAAVCVVLA
                     SDGYPEKYDKGFEIKGLDTFKDKDGYYVFHAGTKFDGDKIVTNGGRVLGVVAKGENLK
                     AARANAYKATEWIDFANKYKRNDIGKAIDEA"
     gene            complement(1380..>1573)
                     /locus_tag="PLC71_10050"
     CDS             complement(1380..>1573)
                     /locus_tag="PLC71_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRM03019.1"
                     /translation="FVDEGTDTGPIIAQKAVEIKQGDTPEVLQRRIMEQAEWVIMPKA
                     IDDIANGRIKVEEGKVIYL"
     assembly_gap    1574..1583
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1584..2013)
                     /locus_tag="PLC71_10055"
     CDS             complement(<1584..2013)
                     /locus_tag="PLC71_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HRM03020.1"
                     /translation="MLKVAVLVSGGGTNLQAIIDGIENGSITNAKIDVVISNNKNAYA
                     LERAKKHDIEAVALSPKDFETRDLFNEALYNELVDRKIDLIVLAGCLVVIPEKIIHEF
                     ENRIINIHPSLIPSFCGTGYYGLKVHEKALARGVKVSGATV"
     gene            complement(2007..>2622)
                     /locus_tag="PLC71_10060"
     CDS             complement(2007..>2622)
                     /locus_tag="PLC71_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRM03021.1"
                     /translation="TAEHPGLMPEEDYDLAGFAVGVCDRKDLITGENLKDGDVLIGMA
                     STGVHSNGFSLVRKVFDMSKESLNTYYDELGKTLGEALLAPTRIYVKALKSVKDAGVT
                     VKACSHITGGGFYENIPRMLPEGMKAVVKKDSYEVPAIFKLLAKEGDIAEEMMYNTFN
                     MGLGMVVAVDKEDVDKTMEAIKAAGDTPYIVGHMEAGEKGVTLC"
BASE COUNT          663 a          628 c          387 g          934 t
ORIGIN      
        1 tcatatatgt gtactactac aaaaaggatg gctgatttct cagccatcct ttttatatga
       61 tcatccttta aagattatgc ttcatcaatt gcttttccga tgtcatttct cttatatttg
      121 tttgcaaaat caatccattc tgttgctttg taagcatttg ctcttgcagc ctttagattt
      181 tctccttttg ctacaacacc taagacacgt cctccatttg tcacgatctt atcaccatca
      241 aactttgttc cagcgtggaa tacataatat ccatctttgt ctttgaatgt atctaatcct
      301 ttgatttcga atcctttatc gtatttctct ggatatccgt cagatgctaa aacaacgcat
      361 actgctgcat tatcttcaaa ttccaattct acatcagata attttccatc aatgcaggct
      421 tccatcacgt cgatgatatc attcttcatt cttggaagaa caacctgtgc ttctggatct
      481 ccaaaacgtg cattgtattc taagactttt ggtccgtctt ctgtgatcat caatcctgta
      541 aataagattc ctgtaaatgg tcttccctca gaagccatcg catcgatcgt tgactgatag
      601 acatattttt cacagaatgc ttctacttca tcattgtaga atggacttgg agagaatgtt
      661 cccattcctc ctgtgttaag tccctgatct ccatctttag cacgtttatg gtcctgtgcg
      721 gatgtcatac atttgatcgt ttttccatca cagaatgcca gaacagatac ttctcttcct
      781 gtcataaact cttcaataac gatttcatta ccagcatcgc caaactgttt gtcaagcatt
      841 aatgtcttaa caccttcttt agcttcttct aatgtgttac agatcaatac accttttcca
      901 agtgctaatc catccgcttt taatacgatt ggcatcttag ctgtttctaa atatttgata
      961 gcttcatctg gatctgtaaa attctcatat gcagctgttg gaatcttata tttcttcatc
     1021 agatctttag aaaatgcttt ggaaccttcg atgatcgctg cattctttct tggtccaaaa
     1081 acacgcagtc cttctgattc aaatgcatcg acaacaccac caactaatgg atcatccatt
     1141 ccaatgatcg taagatcaat tgcattttct ttcgcaaatg caaccagttt atcgaattcc
     1201 attgctttga tatttacaca ttccgcaaat tcagcaattc ctgcatttcc tggtgcacaa
     1261 taaatcttgt ctacttttgg gcttttagcg acagaccatg cgatcgcgtg ttctcttcca
     1321 ccgcttccta cgattaaaac tttcatcttt catttccttt ctttcgtgat ctgaatgatt
     1381 tatagataaa tgacttttcc ttcttcgact ttgatccttc cattagcaat gtcatcaatg
     1441 gctttcggca taataaccca ttctgcctgc tccatgattc ttcgctgaag aacttctgga
     1501 gtatctccct gtttgatctc tactgccttc tgggcaatga tcggacctgt atccgttcct
     1561 tcatcgacaa aatnnnnnnn nnngaacggt tgctccagaa actttaactc cccttgccaa
     1621 ggctttctca tgaaccttca atccataata gcctgttccg caaaatgatg ggatcaatga
     1681 tgggtgaata ttgatgatcc tgttttcaaa ttcatgaatg atcttctccg gaatcacaac
     1741 caagcatccc gcaagaacga tcagatcaat cttacggtct acaagctcgt tatacaatgc
     1801 ttcgttaaat aaatctcttg tctcaaaatc tttgggagaa agtgcaactg cttcgatatc
     1861 atgtttcttc gcacgctcaa gtgcatatgc attcttatta ttgctgatca caacatcaat
     1921 cttcgcattt gtaatgcttc cattctcgat tccatcgata atggcctgaa ggtttgttcc
     1981 tcctccggaa acaagtactg ccacttttag cataaagtaa ctcctttttc tccagcttcc
     2041 atatgcccta cgatatatgg tgtatctcct gctgctttga tcgcttccat tgttttgtca
     2101 acatcttctt tatcaaccgc aacaaccatt ccaagtccca tattgaatgt gttgtacatc
     2161 atctcttctg cgatatctcc ttctttggca agaagtttaa agattgctgg tacttcatag
     2221 ctgtctttct tcacaactgc tttcattcct tctggaagca tacgtgggat attctcatag
     2281 aatccaccac ctgtgatatg actgcatgct ttaactgtaa ctcctgcatc tttcacactc
     2341 tttaatgctt ttacatagat tcttgtcgga gcaagtaatg cttctcctaa tgtttttcct
     2401 aattcatcat aatatgtatt taaagattct ttactcatat caaatacttt tcttactaaa
     2461 gagaatccgt tgctgtgaac tcctgtagat gccataccga tcaagacatc tccatctttt
     2521 aagttttctc ctgtgatcag gtcttttctg tcacatacac caactgcaaa tcctgcaagg
     2581 tcatagtctt cttctggcat aagtcctgga tgttctgctg tc
//
LOCUS       DAOMUE010000288         2621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_11249_length_2621_cov_2.812549, whole genome shotgun sequence.
ACCESSION   DAOMUE010000288 DAOMUE010000000
VERSION     DAOMUE010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11249_length_2621_cov_2.812549"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    200..209
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(316..2040)
                     /locus_tag="PLC71_10065"
     CDS             complement(316..2040)
                     /locus_tag="PLC71_10065"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRM03022.1"
                     /translation="MNYKETYQEWLSNPYFDEDTKKELTAIENDENEIKERFYADLAF
                     GTAGLRGIIGAGINRMNIYVVRKATQGLADYILEQGTDKKRVAIAFDSRHMSPEFADE
                     AARTLAANGIKAFIFESLRPTPELSFAVRHLDCIAGINVTASHNPPQYNGYKVYWEDG
                     AQFTDPHASGVTAKVNAITDISTCKTMSKEDAVTAGLYEVIGKEVDDAYIAEVEKQVI
                     NQASIDEMASKLKIVYTPLHGTGNLPVRRVLDDLGFKNVYVVPEQELPDGDFPTVSYP
                     NPEAKEAFALGLALAKEKDADLVLATDPDADRLGVYVKDAKSGEYIPLTGNMSGSLLC
                     EYVLSQKKEKAGSLPADGAVVKSIVTTNLVDEIAKAYNVKLIEVLTGFKWIGKEILGF
                     EQSGKGTYLFGLEESYGCLIGTYARDKDAVSATVALCEAAAYYATKGKTLWDVMIDMY
                     EKYGYCVDSITAMDFPGLEGMDKMKAMLANLRENPLKDIAGYKVLKIRDYSKDTVLDV
                     ESGNVEPTGLPASDVLYYELEGGAWVCVRPSGTEPKIKFYYGVKASSMDEAQVESAKF
                     AEWAKAQA"
     gene            complement(2097..2546)
                     /locus_tag="PLC71_10070"
     CDS             complement(2097..2546)
                     /locus_tag="PLC71_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03023.1"
                     /translation="MERSIQILNETTPSRRGWCHGAYNHHNIIMTSSAPATIHFEHFY
                     HGYPILDVYYFLKKALEKNNYNFAFCETFLSNYDRFMPLSKDDLLCLYGLFLFPEKFW
                     KISNQYMAHKKHWISPRYIEKLEEFIHNKEARSIFLEKYVQIYNVFS"
BASE COUNT          782 a          530 c          455 g          844 t
ORIGIN      
        1 atttgttgtg atttctgtta atttactatt gacaaaggtc attacatatc agttttcaaa
       61 ggaacaggcg cggatcggcg atttgtaaaa aacgctctta aaatatgcgt gttgccatcg
      121 gttttttata aatatttgtt cttatcttat ttctgtcaca tatactaaag actactcttc
      181 aaatcagaat ttatgcctgn nnnnnnnnna aaaaataaat aaacgacagg aaaagaagca
      241 ttggaatttt gccacgtttc ctgtcgttta tttatttttt atcatatact caaagcctac
      301 agttggccaa ataaactatg cctgagcctt agcccactca gcaaatttag cactttcaac
      361 ctgagcttca tccatactag aagcttttac accatagtag aatttaatct taggttctgt
      421 accagaagga cgaacgcaaa cccaagctcc accttcaagt tcatagtaaa gaacgtcaga
      481 agcaggaagt cctgtaggtt ctacgttacc gctttctaca tcaagaacag tatctttgct
      541 gtaatcacgg atcttaagaa ctttataacc agcgatatct tttaatgggt tttcacgtaa
      601 attagcaagc atagctttca tcttatccat accttctaat cctgggaagt ccattgctgt
      661 gatgctgtct acgcagtatc cgtatttctc atacatgtcg atcataacat cccataatgt
      721 cttacctttt gttgcatagt aagcagcagc ttcacaaaga gcaactgtag cagaaacagc
      781 atctttatct cttgcatatg taccgatcag gcatccgtag ctttcttcta atccgaacag
      841 atatgtacct tttccggact gttcaaatcc aaggatttct ttaccgatcc atttgaatcc
      901 agttaatact tcgatcagct tcacattgta agccttcgcg atctcatcga caaggtttgt
      961 tgtaacgata gatttaacaa cagcaccatc tgctggaaga gatccagctt tttctttctt
     1021 ctggcttaat acatattcgc ataataaaga accagacata ttacctgtta aaggaatata
     1081 ttctccgctc ttagcatctt ttacatatac acctaaacgg tcagcatcag ggtcagtagc
     1141 aagaacaaga tcagcatctt tttctttggc aagagcaaga cctaatgcaa atgcttcttt
     1201 tgcttctggg tttggatagc tgactgttgg gaagtcacca tctggaagtt cctgttctgg
     1261 aacaacatat acattcttaa atccaagatc gtcaagtacg cgacgtactg gaagattacc
     1321 tgttccatgg agaggagtat aaacgatctt taatttggaa gccatctcat cgatggaagc
     1381 ctggttgatc acctgttttt caacttctgc gatataagcg tcatcaactt ctttaccgat
     1441 cacttcatat aaacctgcag ttacggcatc ttctttagac attgtcttgc aagttgaaat
     1501 gtcagtgatc gcattaactt tcgctgtaac acctgaagca tgaggatctg taaactgagc
     1561 accatcttcc cagtatactt tgtatccatt gtactgaggt gggttatggc tggctgttac
     1621 gttgatacca gcgatacagt ctaaatgtct tacggcaaat gataattctg gtgttggacg
     1681 taaggattcg aagataaatg ctttgattcc gttagcagca agagtgcggg ctgcttcgtc
     1741 agcaaattca ggagacatat gtcttgaatc aaatgcgata gcaacacgtt tcttatctgt
     1801 tccctgttct aagatataat cagctaatcc ctgagtagcc tttcttacaa cataaatatt
     1861 catacggttg ataccagcac cgatgattcc acgaagtcca gcagtaccaa atgcaagatc
     1921 tgcgtaaaaa cgttctttga tttcattttc atcgttctcg atggctgtta attctttttt
     1981 tgtatcttca tcaaaataag gattagaaag ccattcctga taagtttctt tgtagttcat
     2041 aaattactcc tcctttaagt tataaaaatg ccttttgttt aattatacct taaaactcat
     2101 gaaaaaacat tataaatttg tacatatttt tcgaggaaaa tcgagcgagc ttctttattg
     2161 tgaataaact cctctaattt ctcgatatat cgtggagaaa tccaatgttt tttgtgtgcc
     2221 atatactgat tcgagatctt ccagaatttt tccggaaata aaaacaatcc atacaggcat
     2281 aaaaggtcat cttttgacaa aggcataaag cggtcatagt ttgataaaaa ggtttcacaa
     2341 aaggcaaaat tataattatt tttctcaagt gccttcttta ggaaataata aacatccaga
     2401 atcggatacc catgataaaa atgctcaaaa tggatcgtag ctggggcaga ggatgtcatg
     2461 atgatattat ggtggttata agccccatga caccatccac ggcgagaagg tgtggtttca
     2521 tttaagattt ggatggaacg ctccatctgt tcccagaagg ctttcgtctg ttccaaaaat
     2581 aaatattcca tttttcaaat ccataatttc attataccgt t
//
LOCUS       DAOMUE010000289         2613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_11289_length_2613_cov_1.889367, whole genome shotgun sequence.
ACCESSION   DAOMUE010000289 DAOMUE010000000
VERSION     DAOMUE010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11289_length_2613_cov_1.889367"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /gene="glmS"
                     /locus_tag="PLC71_10075"
     CDS             complement(<1..1350)
                     /gene="glmS"
                     /locus_tag="PLC71_10075"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRM03024.1"
                     /translation="MCGIIGFTGNLQAPGILVDGLQQLEYRGYDSAGIAVNNGSETKI
                     VKTTGKVATLREKVEATADLAGTCGIGHTRWATHGGVTEVNAHPHVSGNVTLIHNGII
                     ENYKELAASLKTKGFTAISETDTEVAAMLIDSLYDGDPFAALKEADKALEGAYGFCVM
                     FKDHPGEIYCMRNASPLVASHTEDGSFIASDMVALLKYTKDYFVVPEEHIVVMKADSI
                     TLYTMDGGVEEPKMLHVDWDTTAAQKGGYDFFMQKEIYDQPEALHNTIMPRIHNGHAD
                     FSADNISDDIFKGVNRVIVTACGTAWHAGLVGRHLIEAMIRIPVTVELASEFRYMNPI
                     LDEHTLVVVITQSGETADSLAALRLANERGAKTISIVNVKGSSIARESDYVLYTHAGP
                     EIAVASTKAFSVQCAGMYLIALKLAEVNNLMTDEQMKDFCDKFEQAIGTVEEVLKMDD
                     "
     gene            2029..2214
                     /locus_tag="PLC71_10080"
     CDS             2029..2214
                     /locus_tag="PLC71_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03025.1"
                     /translation="MRKMKKILAWLTVLTVNVSMMPMPTVAAATATDASTALNEETIT
                     SDATKEATQAQETERKL"
BASE COUNT          864 a          495 c          481 g          773 t
ORIGIN      
        1 atcatccatc tttaagactt cttcaacagt tccgatcgcc tgttcaaatt tatcacagaa
       61 atctttcatc tgttcatctg tcattaagtt gttaacttct gcaagtttta atgcgatcag
      121 atacatacct gcacactgta cagagaacgc ttttgtactt gcaacagcga tctctggacc
      181 tgcatgtgta tataatacat aatcactttc acgagcgata cttgatcctt ttacgttaac
      241 gatagagatc gtttttgctc cacgttcatt tgcaagacga agtgcagcaa gggaatcagc
      301 tgtttcacca gactgtgtga tgacaacaac taaagtatgt tcatctaaga tcggattcat
      361 gtaacggaat tcagaagcta attcaacagt tacaggaata cggatcatag cttcgatcag
      421 atgtcttcca actaatcctg catgccatgc agtaccacaa gctgtcacga tcacacggtt
      481 tacaccttta aagatatcat cagagatgtt atctgcacta aagtcagcgt gaccattatg
      541 gatacgaggc atgattgtat tatgaagagc ctctggctga tcatagatct ctttctgcat
      601 aaagaaatca tatccgcctt tctgggcagc tgttgtatcc cagtcaacat gaagcatctt
      661 aggttcttca accccaccat ccattgtgta taatgtaata ctgtcagctt tcatgacaac
      721 gatatgttct tcaggtacta cgaaataatc ttttgtatat tttaataatg caaccatatc
      781 agaagcgatg aaagaaccat cttctgtatg agatgctact aatggacttg catttctcat
      841 gcaatagatc tctcctggat gatctttgaa cataacacag aaaccataag ctccttctaa
      901 agctttatca gcttctttta atgctgcaaa tggatctcca tcatataaag agtcgatcag
      961 cattgctgca acttcggtat ctgtttcaga aatagcagta aatccttttg tttttaagga
     1021 agcagctaat tctttgtagt tttcaatgat tccgttatgg atcagagtta catttccaga
     1081 tacatgagga tgtgcgttaa cttctgtaac accaccatga gttgcccagc gagtatgtcc
     1141 gataccgcat gttccagcaa ggtcagcagt agcttcaact ttctcacgaa gtgttgcaac
     1201 ttttccagtt gtttttacga tcttagtttc tgatccatta tttactgcga ttccggcact
     1261 gtcatatcct ctgtattcta actgctgtaa tccatcaact aaaattccag gtgcctgtaa
     1321 atttccagta aatccaataa ttccacacat aatataaatc tccttaaaat tgcgcaaaga
     1381 aaataaaagg aagtatagat atgtaaaaaa cttccaaata aatcttttgt tttctttttc
     1441 taaatcatat gcggttttgt ccagattata ctgcctctgt ttcatgatca ctcatgggtt
     1501 tactcagtat atcacgtagt ctggagctgc gggaaagcat ccgccgaatc tttcgatcac
     1561 tttcctcctc gttaaccttc ctatcagaac ctcatcggtc tgtttccgta tcaggaaggt
     1621 gcatggcgct taaattctgt ataactccct tcgaacatat tttgtcaaaa actcaaaacc
     1681 caaagttttt aacgctagtg attttaacac ggaatatgtg tgaagtcaaa tgagaacgtt
     1741 gtattttata aaaacttaat aagtttatgt gaatgaaatg aatttaccta ttaaaaagaa
     1801 agtgtattta aaacagaaga agtaattgac gaaaagtacc aataaatgta taatatacat
     1861 gtgatgatga atacaaatga tcgcaatttg tttgcgatat tttccttagg tcaggaagat
     1921 atcgcatata tttttgacat ttgtattata ggggggttat agggctcgat gagttatata
     1981 accatagaac aaaataatta acacatgagt tcatgagaag gaggaactat gaggaagatg
     2041 aagaagattc tggcatggtt aacagttttg acagttaatg tcagcatgat gcctatgcca
     2101 acagtagcag ctgctactgc tacagacgct tccacagcat taaatgaaga gacaataact
     2161 tccgatgcaa ctaaagaagc aacacaggcg caagaaacag aaagaaagct gtagaaagcg
     2221 caacaaaagc agcagctacg acagcagcaa agaaagtaac aacagaaacg aagaaggcag
     2281 ggagcaaatc aaaatctgca gcttcaacac agagtttaca ggatacgatt gaagaaggaa
     2341 tcgtcaaaga tcgtgatcaa acaaagattg atgtatctga ttatgatgca tctaaagcag
     2401 aagtaaagaa agctacaaac aaagtattaa aagataatag tctgtctaaa ttaacagatg
     2461 ttacttatga tacaaattct gaaggaaaag tcgaaactgt aaatgttgag acggatgtaa
     2521 cttatttaat ggcagttgat gaattagaag caatcaacga tgctgctagt gatgaggata
     2581 aggcttcaga agctacaatg gaagaagtag aga
//
LOCUS       DAOMUE010000290         2576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_11504_length_2576_cov_2.442285, whole genome shotgun sequence.
ACCESSION   DAOMUE010000290 DAOMUE010000000
VERSION     DAOMUE010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11504_length_2576_cov_2.442285"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1977
                     /locus_tag="PLC71_10085"
     CDS             103..1977
                     /locus_tag="PLC71_10085"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022872 -
                     protein-N(PI)-phosphohistidine-mannitol phosphotransferase
                     system transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HRM03026.1"
                     /translation="MKNGLQKFGKFLSAMVMPNIGAFIAWGFITALFIADGWIPNAKL
                     ASIQPYMLTFLLPMLIAYTGGKQVGGDRGGVMGAIAVMGCIAGVGGFDGQPMLMGAMV
                     MGPFAGWVIKKFDKAMDGHMPAGFEMLINNFSVGILGMIVAIIGYFIIGPFMSTVLAV
                     LTAGVNVLVKAKLIPLAAIFIEPAKVLFLNNAINHGIFTPIGIEQAKEAGKSIMYMLE
                     ANPGPGLGVLLAYAVFSKDKVTKSSAPGAIIIHFFGGIHEIYFPYILMNPIVIIAPIV
                     GNICAITFFTFTKCGLIGPSSPGSIIAYLSMSPKSQIPLTILGVLIATVVSFLIASPI
                     IRMSDGKSLEDAQDDMAAKKAESKGITVDPGEKKDADKVKKIVFACDAGMGSSAMGAT
                     KFRNRVKAQRPDLTVINTSVDNIPADCDIAVVQAVLADRARKSAPQAQLVIINNFLQD
                     PNLDGLYEQMTAKVDASAQTAAAVEEVETPVATGNAKILVEEGIKTGLKPVDKFEAIK
                     AAGKLLNELGYVEEGYIDAMVKREETVTTYMGLGVAIPHGTGDAKKKVQKTGIVMLQY
                     PEGVDFDGEKAQLVFGIAGIGDEHLGLLAKISEIIEDPERLEQLKTTKNVDEIMEMFK
                     "
     gene            2086..>2576
                     /locus_tag="PLC71_10090"
     CDS             2086..>2576
                     /locus_tag="PLC71_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HRM03027.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLSKAGYHVVFADVNQKIIDKINED
                     KKYTIFVKDVESSEIVITDISGVNSTKPELIDEVKEAEIITTAVGVRILPIIAPSIAE
                     GIKARKENGSEEYLNIIACENAVKASSQLKEAVYGNLNDEEKAYADKYVGFPDCSVDR
                     IVPP"
BASE COUNT          915 a          428 c          554 g          679 t
ORIGIN      
        1 tctcttaccc atctatatgg tttggtccat aaagataagg ttgtagagat ttacaatatg
       61 caaaatgaag agaagataga aagcaaaagg aggatattgt aaatgaaaaa tggattacaa
      121 aaatttggta aatttttaag tgcaatggta atgcctaata tcggagcatt cattgcatgg
      181 ggattcatta cagcattatt tatcgcagat ggatggattc caaatgcgaa gttagcatca
      241 attcagccat acatgttaac attcttatta ccaatgctaa tcgcctacac aggtggtaaa
      301 caggtcggtg gagaccgagg aggagttatg ggtgcaatcg cagtcatggg ttgtatcgcc
      361 ggagttggtg gatttgatgg acagccaatg ttaatgggag ccatggtaat gggaccattt
      421 gcaggatggg tcatcaagaa atttgataaa gcaatggacg gacatatgcc agccggattt
      481 gagatgttga tcaataactt ctctgttggt attttaggaa tgatcgtagc gatcattgga
      541 tactttatca ttggaccatt tatgtcaacc gtattagcag tgttaacagc aggagttaac
      601 gtacttgtaa aagcaaaatt aataccttta gcagcaatct ttatcgaacc tgccaaagta
      661 ttattcttaa ataacgcgat caaccatggt atctttacac caattggtat tgaacaggca
      721 aaagaagctg gtaaatctat catgtatatg ttagaggcaa acccaggacc aggacttgga
      781 gtattattag catatgcagt attctctaaa gacaaagtaa caaaatcatc tgcaccaggt
      841 gcgatcatca ttcatttctt cggtggtatt catgagattt acttcccata tatcctgatg
      901 aacccaatcg tgatcatcgc tccgatcgtt ggtaacattt gtgcgatcac attcttcaca
      961 tttacaaaat gcggacttat tggaccatct tcaccaggat caatcatcgc ttatttatca
     1021 atgtcaccaa agagccagat tccattaaca atcttaggtg tactgatcgc aactgtagta
     1081 tcattcttga tcgcatcacc tatcattcgt atgtcagatg gtaagagtct tgaagatgca
     1141 caggacgata tggcagcaaa gaaagcagaa tctaaaggaa tcacagttga cccaggagag
     1201 aagaaagacg cagataaagt taagaagatc gtatttgcat gtgacgcagg aatgggatca
     1261 tctgcaatgg gagctacgaa attcagaaac cgtgtgaaag cacagagacc agatcttaca
     1321 gttatcaata catctgtaga taatattcca gcagattgtg atatcgcagt tgttcaggca
     1381 gttttagcag acagagctag aaaatctgca ccacaggcac agttagtaat tatcaataac
     1441 ttcttacagg atccaaactt agacggatta tatgaacaga tgacagcaaa agttgatgca
     1501 tcagcacaga cagctgcagc tgttgaagaa gttgaaacac cagtagctac tggaaatgca
     1561 aagatccttg tagaagaagg aatcaaaaca ggattaaaac cagtcgacaa attcgaagcg
     1621 atcaaagcag caggaaaact gttaaatgaa cttggatatg ttgaagaagg ttatattgac
     1681 gctatggtaa aacgtgaaga aacagttaca acatacatgg gattaggtgt agcaattcct
     1741 cacggaacag gagatgccaa gaagaaagta cagaaaactg gtatcgtaat gcttcagtat
     1801 ccagaaggtg tagactttga tggagaaaaa gctcagttag tcttcggtat tgctggaatc
     1861 ggtgatgaac atcttggatt attagctaag atctctgaga tcatcgaaga tcctgaaaga
     1921 ttagaacagt taaaaacaac aaaaaacgtt gacgaaatca tggaaatgtt taaataagat
     1981 ttaaacaaaa aaaggtcaat ttaataagcg ggaaattctt ctcgcttatt aaatacctaa
     2041 gatatatact tatagattta tattttaatt ttaggagtga agaaaatgaa gaaagcaatt
     2101 cagtttggag caggaaatat tggacgtgga tttatcggtg gactgttaag caaagcagga
     2161 tatcatgtag tattcgcaga cgtaaatcag aaaatcattg ataagatcaa cgaagataag
     2221 aaatatacaa tttttgtaaa agacgtagaa agcagcgaga tcgtgatcac agatatcagc
     2281 ggagtaaatt caacaaaacc tgaactgatc gatgaagtaa aagaggctga gatcatcaca
     2341 acagccgttg gagtaagaat tcttccgatc attgctccat ctatcgcaga aggaatcaaa
     2401 gcaagaaaag aaaatggttc tgaagaatac ttaaacatca ttgcttgcga aaatgcagtc
     2461 aaagcaagtt ctcagttaaa agaagctgtt tatgggaact taaatgacga agaaaaagca
     2521 tacgcagaca aatatgttgg attcccagac tgctccgtag atagaatcgt acctcc
//
LOCUS       DAOMUE010000291         2569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_11551_length_2569_cov_2.670644, whole genome shotgun sequence.
ACCESSION   DAOMUE010000291 DAOMUE010000000
VERSION     DAOMUE010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11551_length_2569_cov_2.670644"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1448
                     /gene="rpoN"
                     /locus_tag="PLC71_10095"
     CDS             153..1448
                     /gene="rpoN"
                     /locus_tag="PLC71_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRM03028.1"
                     /translation="MSYEMKLEQGLSQQLNQFQVQSLKILSFDNYELEKFLQDEYAEN
                     PLMEHKESGKEFFTLHGNAGGYQDIEQSDIEDKKIEDPEAFFMEQLNAKDYTKKEWNV
                     MKYMAQCLEESGLLLVSTKEISHKLGIEEKDCERLLDHLKQLEPAGVFAESVSECLLL
                     QLEREGILNDDLRYIVENHLEDIAMGHLSTVSRALDIKTSQVRKYIFKIQELNPRPLS
                     GYTGGTDQYIVPDILMEKDGEELKIRLNDDWIGDYSINDYYVKMMEQAEDSELKEYFR
                     KKYERCRFIITSIEQRRETMLKISNAILDCQREYFMNHKQLKPMTMQNIADDIEMHVS
                     TVSRAVKGKYIQYPGGTIGMRDIFNNTQTFGDQDSTTEEIKEEIRRLVKEEDKKKPLS
                     DSKIVSLLEQKNMKISRRTVAKYRAQLGIAGTTQRKIHE"
     gene            1607..2317
                     /locus_tag="PLC71_10100"
     CDS             1607..2317
                     /locus_tag="PLC71_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRM03029.1"
                     /translation="MRLNKMTGRVIATMGIAAMMMTQTAGVMAAEQTENKQLKVVYYN
                     QADYPGKKIGGSTIQAAGCGPTAVAVCYSSLTGKKADVPKMCKQAYKHGWYYTGQGCS
                     HSVVPGLSKLYGMECKGLGMDKDAVEKALRAGHPVVALMGPGDFTKNGHFVVLTRMVG
                     KDKVKIADVGSRARTAETWSLKKVIRQGKEGANAGGPFWEISVKEEKQEEPDYKQKML
                     DGHKNIDAVTNAIDKIAD"
     gene            complement(2258..>2569)
                     /locus_tag="PLC71_10105"
     CDS             complement(2258..>2569)
                     /locus_tag="PLC71_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03030.1"
                     /translation="LCGLFFFWKGAKGELSYKARKNPALQDSPPAAGRFCDILPFRRS
                     AILPGALFHIIRDSIGISYDIKKNIPQNISFMKCSFLFLILIRNLIDRICHSIYVFVT
                     I"
BASE COUNT          982 a          365 c          592 g          630 t
ORIGIN      
        1 tcagaagacc atagaagaag agaaagcgag aaaatagata taagataact tagaaaaaga
       61 gatcatccaa gcaaaatctc ttttattcag agtgaaaata gattatgata agattaagta
      121 cttgatcaaa gaaagtaatt ggaggatgta aaatgtcata tgaaatgaag ctggaacaag
      181 gcttgtcaca gcaattaaat cagtttcagg tacaatcatt aaagatctta tcgtttgata
      241 attatgaact tgagaagttt ttgcaggatg aatatgcaga gaatccattg atggaacata
      301 aggaatctgg aaaggaattc tttacattgc atggaaatgc aggcggatat caggatattg
      361 agcaaagtga tattgaagat aagaaaatcg aagatcccga agccttcttt atggaacagt
      421 taaatgcaaa agattataca aagaaagagt ggaatgttat gaaatacatg gcacaatgcc
      481 tagaggaatc cgggcttttg cttgtttcca caaaagagat cagtcacaag cttggaattg
      541 aagaaaaaga ttgtgaaagg cttttagatc atctcaagca attagaacca gcaggtgtat
      601 ttgcagaaag tgtatcggaa tgtctccttc ttcagcttga aagagaagga atattaaatg
      661 atgacctgcg atatatcgta gaaaatcacc tggaagatat cgcaatggga catttatcga
      721 ctgtttcaag agcacttgat attaagacat cacaggtaag aaagtatatt tttaaaattc
      781 aagaattaaa tccaagaccg ttaagtggat atacaggggg aaccgatcag tatatcgtcc
      841 cagacatttt aatggaaaaa gatggagaag aactaaagat tcgtttaaat gatgactgga
      901 tcggtgatta ttcaatcaat gattattatg tcaaaatgat ggaacaggca gaagattctg
      961 agctaaaaga atatttcaga aagaaatatg agagatgtcg ttttatcatt acaagtattg
     1021 aacaaagaag agagacgatg cttaagattt caaatgcaat cctagattgt cagagagaat
     1081 attttatgaa ccataagcag ttgaaaccaa tgactatgca aaatatagca gatgatattg
     1141 aaatgcatgt atcaacggta agtcgcgcag tcaaaggaaa gtatattcaa tatccaggtg
     1201 gaacgatcgg aatgagagac atttttaata atacacagac atttggagac caagattcga
     1261 cgacagaaga gattaaggaa gaaatcagac ggcttgtgaa agaagaagat aagaaaaaac
     1321 cattaagtga cagtaagatc gtaagtttgt tagaacaaaa gaatatgaag atttcaagaa
     1381 gaaccgtagc aaaatatcgt gcacagcttg gaattgcagg aacgacacag cgaaaaattc
     1441 atgaataaat gcagcgacat ttatcatctt ttggcaaaaa aatgaaaaaa tgtaaatgag
     1501 atttaataaa aatttaacaa ttgaaacact tgattaacaa actaccctct gttataatta
     1561 ataactgatg tattatgtca aaagcagaag ttatctggag ggtactatga gattgaacaa
     1621 gatgactggc agagtgatcg caacgatggg gatcgcagca atgatgatga cacagacagc
     1681 aggggttatg gctgcagagc agacagagaa caagcaatta aaagttgttt attataatca
     1741 ggcagattat ccgggaaaga agatcggcgg aagtacgatt caggcagcag gatgcggacc
     1801 aaccgcagtt gctgtatgct acagttcttt aactggaaag aaagctgatg ttccaaagat
     1861 gtgcaaacag gcatataaac atggatggta ttacacagga cagggatgtt ctcacagcgt
     1921 agttccagga ttatccaaat tatacggaat ggagtgtaaa ggtctcggaa tggataaaga
     1981 tgctgtagag aaagcattaa gagcaggaca tccagtcgtg gcactgatgg ggcctggaga
     2041 tttcacaaag aatggacatt ttgttgtatt aacaagaatg gtcggcaaag ataaagtaaa
     2101 gattgccgat gtaggaagca gagccagaac agctgagacg tggtcgctta agaaagtgat
     2161 ccgacaaggc aaagaaggtg ctaatgcagg gggaccattc tgggagatca gtgtgaaaga
     2221 agagaagcag gaagaaccag attataaaca aaagatgtta gatggtcaca aaaacataga
     2281 tgctgtgaca aatgcgatcg ataagattgc ggattagaat taaaaataaa aaagaacatt
     2341 tcataaatga gatattttgt ggaatgttct tttttatatc ataggaaatt ccgatggaat
     2401 cccttatgat atgaaaaaga gctccgggca ggatcgcact gcggcggaag ggtaatatgt
     2461 cgcagaagcg acccgccgca ggcggagaat cctgtaaagc aggattcttt cttgctttat
     2521 aggaaagttc gcctttggct cctttccaaa agaaaaatag cccacataa
//
LOCUS       DAOMUE010000292         2549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_11660_length_2549_cov_2.596632, whole genome shotgun sequence.
ACCESSION   DAOMUE010000292 DAOMUE010000000
VERSION     DAOMUE010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11660_length_2549_cov_2.596632"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..947)
                     /locus_tag="PLC71_10110"
     CDS             complement(141..947)
                     /locus_tag="PLC71_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HRM03031.1"
                     /translation="MKKFLVGLLTVALAASMLVGCGKKEEKTKTDNSLKDVQSKKELV
                     LGLDSSFPPMGFTDKKDNIVGFDIDLAKEVTKRMGIKLKLQPINWNSKEQELNTEKVD
                     CLWNGVTYSKARAKEMLCSESYMDNHQVLIVPANSKIKSLKDITKDTEVGIQSGSTAA
                     DAADSVKELKNSKIKLLADNVQILNDLGHGLDAAVMDEVVAKYYTKKDAGKYKVLEDS
                     VAKEKYVIGFRKDDKALCNEVVKQLKAMAKDGTLKKISEKWFDEDITTIK"
     gene            1138..2484
                     /locus_tag="PLC71_10115"
     CDS             1138..2484
                     /locus_tag="PLC71_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HRM03032.1"
                     /translation="MYYKKILAAALTVSCIGGIVPANSVQAASLKIRYSGKTRYYKGK
                     QLHVSYANKNLSSKYVGIQINKVNMIPYYDYLVKQGPKVKRTYSKSSGKLILKNGDDT
                     LTAYVGKRTYYLNGVKKTFSVAPTKVKYYKTKKTIILIPANAIVKGLGLNYKYSSSSK
                     RIYITQPVIPSDSTTQVQTQPSGSVQYTNYNKSLSAYVTAEKKQHPTYGGKSISTSTY
                     TSYVDPSKDTTNNFQFLTLDTYREVDPTAYNNLLNSKLRSNSVLRNKGNVLIAAAKQY
                     NIDPVYLLCQTILETGYGTSTLSQGKAITTVVSGSSVVRDSSGNVTGFKTVNGKYITS
                     TISKKMVYNRYGIKAYDSNPQLCGFSYAYYQGWTSVDAAIYGAAKYVSQDYIHNQTYH
                     QNTLYKFRYNPNINYLWHEYATDPSYAKQIATIMYDQFRSVYRSDVSFTYDKPHFN"
BASE COUNT          800 a          488 c          388 g          873 t
ORIGIN      
        1 ccatccataa ttttctactc ctatattact tatttttatc tatatttata agggacgggt
       61 tattccttta taatatcttt ataaacggaa aaagggcttt agcacgtaag ccctttcttc
      121 cttaaaattt atttctttaa ttatttgatt gttgtgatgt cttcatcaaa ccatttttcg
      181 cttatcttct ttaatgtacc atcttttgcc attgctttta actgttttac aacttcgttg
      241 caaagtgctt tgtcatcttt tctgaatcca atgacatatt tttcttttgc tacactgtct
      301 tctaatactt tatatttacc agcatctttc tttgtgtagt attttgcaac aacttcatcc
      361 ataacagccg catctaatcc gtgtcctaaa tcatttaaaa tctgaacatt gtctgctaac
      421 agtttgatct ttgaattctt taattctttt acactatctg ctgcatcagc tgctgtagat
      481 cctgactgaa ttccaacttc tgtatctttt gtaatgtctt ttaaagattt gatcttactg
      541 tttgctggta caatcagtac ctgatggtta tccatgtaag attctgaaca taacatttct
      601 tttgctctgg ctttgctgta tgtaacacca ttccataaac agtcaacttt ctctgtatta
      661 agttcctgtt ctttggaatt ccagttgatt ggctgtaatt ttaatttgat tcccattctc
      721 tttgttactt cttttgcaag gtcgatatca aatcctacaa tattatcttt cttatctgta
      781 aatcccattg gtggaaagga tgaatcaagt ccaagaacta attctttctt agactgtaca
      841 tctttcaatg agttatctgt ctttgttttc tcttctttct ttccgcatcc tacgagcata
      901 gatgctgcaa gtgcgactgt taataacccg actaaaaact ttttcataac tttatcgctc
      961 ctttatatat tttattcaat cagcacacta ccatgctaaa gtgtttgttt tataatagca
     1021 aagttctctt gctttgtcaa accttatcct ctgttttttg ttacatttct attacatatt
     1081 tattgagttt ccatgaatct tgtgttattc tagaagagta taaaaggagg agtatcaatg
     1141 tattataaaa aaatacttgc tgcagcatta actgtttcat gtatcggtgg tatcgtacct
     1201 gcaaactctg tacaggcagc aagtcttaag atccgctaca gcggcaaaac acgttactat
     1261 aaaggaaaac aattacatgt cagctatgca aataagaacc tgtcttctaa atatgttggc
     1321 attcagatca ataaagtcaa tatgatccct tattatgact atcttgtaaa gcagggtccg
     1381 aaagtcaaac gtacttactc taagagcagt ggaaaactga tccttaagaa tggcgatgac
     1441 acactgacag cttatgtagg aaaacgtaca tactacttaa atggggtaaa gaaaaccttc
     1501 agcgttgccc ctacaaaggt aaaatattat aagacaaaga agactatcat cttaattcct
     1561 gctaatgcga tcgtcaaagg actgggatta aattataagt atagttcttc ttcaaaaagg
     1621 atctatatca cacagcctgt cattccttca gattcaacaa cacaggtcca aacacaacca
     1681 agtggttctg tgcagtatac aaattacaat aagagtttat cagcttatgt aactgcagag
     1741 aagaaacaac atccaactta cggcggcaag agcatcagta catcaactta cacaagctat
     1801 gtcgatccat caaaggatac aacaaataat tttcagttct taacactgga tacataccgt
     1861 gaagttgatc caactgcata taataactta ttaaatagta aattaagatc aaacagtgtc
     1921 ttaagaaata aaggaaatgt cttgatcgct gctgcaaaac agtataatat cgatcctgta
     1981 tatttactat gccagacaat tcttgaaact ggatatggta caagcacctt atctcaggga
     2041 aaagcgatca caaccgttgt ttctggaagt tctgttgtaa gagatagttc tggaaatgtc
     2101 acaggattta agacagtcaa tggaaaatat ataacatcca caatttctaa gaagatggtg
     2161 tacaaccgct acggaatcaa agcctatgat agtaatcctc agctttgcgg attcagctat
     2221 gcatattatc aaggctggac aagtgtcgat gcagcaattt atggtgccgc taagtatgta
     2281 agccaagact atatccataa tcagacttat catcaaaata cactttataa attccgttac
     2341 aacccaaata tcaattatct atggcacgaa tacgcaacag atccatccta tgcaaaacag
     2401 atcgctacga tcatgtatga tcagttccgt agtgtttata gatcagatgt gtcatttaca
     2461 tatgataaac cacacttcaa ttagattttt aattttgtag ttggccaact gtagattttg
     2521 agtatatgat aaaaaataaa taaacgatc
//
LOCUS       DAOMUE010000293         2535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_11744_length_2535_cov_1.560887, whole genome shotgun sequence.
ACCESSION   DAOMUE010000293 DAOMUE010000000
VERSION     DAOMUE010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11744_length_2535_cov_1.560887"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            546..>2535
                     /locus_tag="PLC71_10120"
     CDS             546..>2535
                     /locus_tag="PLC71_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HRM03033.1"
                     /translation="MEDTKTCLEKQPLSQEMLEKMNAYWRAANYLSAGQLYLLDNPLL
                     KEPLTMDQIKKKIVGHWGTVPGQNFIYVHCNREIKRYDLDMILLSGPGHGGNFLIANT
                     YLEGSYSEVYPNISQDEEGMKKMFKQFSFPCGVPSHCAPETPGSINEGGELGYSIAHA
                     FGAVFDNPDLIATVVVGDGEAETGPLATSWQSNKFLNPVTDGAVLPILHLNGYKISNP
                     TIFSRISHEEVENFFKGCGWKPYFVEGDDPMTMHKKMAETMDTVIEEIKAIQKNAREN
                     NDPERPVWPMIVLRTPKGWTGPKVVDGQQIEGSFRAHQVPLTMESPEHLELLKEWLES
                     YHPEELFDENGHLIPELAELAPKGDARLGANPHANGGLLLKDLRLPDFRSYGIEVEPG
                     KTKAQDMIELGGYIRDIFELNEENKNFRIFGPDESMSNRLYKVFETQNRDWNAGLLDT
                     DDCLARNGRIMDGMLSEHMCEGWLEGYLLTGRHGFFASYEAFIRIVDSMAAQHAKWLK
                     VCNQLSWRQPIASLNFILTSNVWQQDHNGFTHQDPGFLDHIANKKADVVRMYLPPDAN
                     CLLSCFDHCIKSKNYVNAIVASKHPSCQWLSMEQAVKHCTQGIGIWDWASNDCGEEPD
                     VVMACCGDTPTLETMAAVTILRDEMPELKIRVVNVVDLF"
BASE COUNT          745 a          505 c          519 g          766 t
ORIGIN      
        1 tgttactttt ttgagaactc tcttcataat gtctcctccg ttttctattt atttacttga
       61 tgattcaatt atatacttaa cctttccggt ttcgcaacaa aatacaacca ttttttctca
      121 caaagttgta ttgttacaag ttgtttttca ttgcttttat gtgtgttttt ctatcttttt
      181 gtgcgttttg acagtcttag tcgctataat tgtgaacaaa aaatgaattt tttgtgcact
      241 ttttggcttt ttcttcatat tttctttaga aggcaataca actttcattt ttccatttac
      301 ttgtattgtt tttgacaaat atgtcgaatt agattgtatt ttatttttcg ttcgctctaa
      361 atccttttcg aactaaatcc ttttatatat gtagatactt ctcttccttt gtatttttat
      421 ccgtacatcc atatcaaaaa attataatct taatgcataa ttgctatttt acgtattgtt
      481 cttattatga tatcctacat ttggttaaat aattaacaca aagtttttat gaaagaggta
      541 agaatatgga agacacaaaa acatgtcttg agaagcagcc gttatctcag gaaatgcttg
      601 aaaagatgaa tgcttactgg cgtgctgcca actatctgtc agccggtcag ttatatcttc
      661 ttgacaatcc gctcttaaaa gagccactta caatggatca gatcaagaaa aaaattgttg
      721 gtcactgggg aactgtccca ggacagaact ttatctatgt acactgcaac cgtgaaatca
      781 aacgttatga tttggatatg atcttattat caggtcctgg acatggcgga aacttcttaa
      841 ttgccaatac atatctggaa ggttcttaca gcgaagtata tccgaacatc agccaggatg
      901 aagaaggtat gaagaagatg ttcaagcagt tctcattccc atgtggagtt ccgagtcact
      961 gtgcaccaga gacaccagga tctatcaacg aaggtggaga gcttggatac tctatcgctc
     1021 atgcattcgg tgctgtattc gacaaccctg atctgatcgc aactgttgtt gttggtgacg
     1081 gcgaagctga gaccggaccg ttagctactt catggcagtc taacaagttc ctgaacccag
     1141 ttacagacgg tgctgtactt ccaattcttc atttaaatgg atataagatc agtaacccta
     1201 ctatcttctc tcgtatttcc cacgaggaag ttgagaactt cttcaaagga tgtggatgga
     1261 aaccttactt cgtagaaggc gatgatccga tgacaatgca caagaagatg gcagaaacaa
     1321 tggatactgt tatcgaagag atcaaagcaa tccagaaaaa tgcacgtgag aacaatgatc
     1381 cagaacgtcc ggtatggcca atgatcgttc tgcgtacacc aaagggatgg actggaccaa
     1441 aggttgtaga tggacagcag attgaaggat ctttccgtgc acatcaggta ccacttacaa
     1501 tggaaagtcc agaacatctg gaactcttaa aagagtggct ggaaagctat catccggaag
     1561 aactcttcga tgaaaacgga cacctgatcc cagaacttgc ggaacttgct cctaagggag
     1621 atgcccgtct cggagctaac ccacatgcaa atggtggatt attattaaaa gacttaagac
     1681 ttcctgattt ccgttcttat ggaatcgaag ttgaaccagg taagacaaag gctcaggata
     1741 tgatcgaact tggcggatat atccgtgaca tcttcgaatt aaacgaagag aacaagaact
     1801 tccgtatctt cggacctgac gaaagtatgt ctaaccgttt atacaaagta tttgaaacac
     1861 agaaccgtga ctggaatgcc ggacttcttg acacagacga ttgtcttgca agaaacggac
     1921 gtatcatgga tggtatgtta agtgagcata tgtgtgaagg atggctggaa ggttatctgt
     1981 taacaggacg tcacggattc ttcgcaagtt acgaagcatt catccgtatc gttgactcta
     2041 tggcagctca gcatgcaaaa tggttaaaag tatgtaacca gctttcatgg agacagccaa
     2101 ttgcttctct taacttcatc ctgacatcaa atgtatggca gcaggatcac aacggattta
     2161 cacatcagga cccaggattc ctggatcaca tcgctaataa aaaagcagat gttgtccgca
     2221 tgtatctccc accggatgct aactgcttac tctcttgctt cgatcactgt atcaagagca
     2281 agaactatgt caacgctatc gtagcttcaa aacacccaag ctgccagtgg ttatctatgg
     2341 aacaggctgt taagcactgt acacagggta tcggtatctg ggattgggca agtaacgact
     2401 gtggtgaaga accagatgtt gttatggctt gctgcggtga cacaccaact cttgaaacaa
     2461 tggctgctgt tacaattctt cgtgatgaaa tgccagaact caagattcgt gttgttaacg
     2521 ttgttgacct gttca
//
LOCUS       DAOMUE010000294         2529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_11786_length_2529_cov_1.984640, whole genome shotgun sequence.
ACCESSION   DAOMUE010000294 DAOMUE010000000
VERSION     DAOMUE010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11786_length_2529_cov_1.984640"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..721)
                     /locus_tag="PLC71_10125"
     CDS             complement(59..721)
                     /locus_tag="PLC71_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HRM03034.1"
                     /translation="MENEKPILRGELALMAAIVINSFSVVLMLYSGSGISAISSVPYA
                     FSEVFKELSLGTWTYIFQGALVLSLMILRRKFVPSYLFSFVAGFAFSELLDVNELWIG
                     ILPQTIPCRIAYFLISYFLLSIGIALENRCQLPIIPTDLFPRELSEIIDKPYARVKII
                     FDVLCLATTGLLTGLMLGYLDGLGIGTIVAAFTMGKMVSITGSWIDQRVTFASVLSKQ
                     TI"
     gene            998..2383
                     /locus_tag="PLC71_10130"
     CDS             998..2383
                     /locus_tag="PLC71_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HRM03035.1"
                     /translation="MLELIQSVNSAVNNFIWGVPAMICIIGVGLLLTIKTKFLQIRKF
                     PYAMKETLGRVFKKQDASDGSMTPFQAVCTALASTVGTGNIAGVAGAIAIGGPGAVFW
                     MWISAILGMCTKFSEVTLAVHFRERNQEGDYVGGPMYYIKNGLSKNWHFLAVLFSAFG
                     VLTVFGTGNATQVNTITTAINSALLNFHVISQSSVGTTNLIIGIIVAILVALILLGGI
                     KRIGQVAERLVPFMAFIYIFFALGVVVLNIDQLPAVFGSIINGAFHPASVTGGIVGSF
                     FMSMKKGVARGIFSNEAGLGTGSIAHACADTKEPVKQGLLGIFEVFTDTILICTLTAL
                     VILCSGIPIGFGADAGAELTISGFTSTYGNWISIFTAVALCCFAFSTIIGWGLYGARC
                     IEFLFSSKVIKPFMLVYSLVAILGATVDLGMLWSIAETFNGLMAIPNLIALFLLSGTV
                     VKLVKDYFHEK"
     gene            complement(2371..2520)
                     /locus_tag="PLC71_10135"
     CDS             complement(2371..2520)
                     /locus_tag="PLC71_10135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HRM03036.1"
                     /translation="MKGNNISISIGISTAPQDGDCYMDLYKRADQALYQAKRSGKARV
                     CNYFS"
BASE COUNT          690 a          490 c          481 g          868 t
ORIGIN      
        1 aactatcacc cgttctatct tattttatca tctttcttat ttctattctc ttaattccct
       61 atatcgtctg tttacttaag accgatgcaa aagtaactct ctgatcgatc cagctgcctg
      121 tgatcgaaac cattttcccc atcgtaaatg cagcaacgat cgttccaata cctaatccat
      181 ctaaatatcc aagcatcaga cctgttaaaa gcccggttgt ggcaagacat aaaacatcaa
      241 atatgatctt aacccttgca tatggtttgt caatgatttc tgataattct cttggaaata
      301 aatccgttgg aatgatcgga agctggcatc tgttctctaa agcaattcct atacttaata
      361 agaaataact gatcagaaaa tatgcaatcc tgcacggaat cgtctgtggc aggatcccga
      421 tccataattc attgacatct aataattcac taaacgcaaa tcctgcaacg aagctgaata
      481 aataagatgg tacaaatttt cttcttaaga tcatcagact taacactaat gctccctgaa
      541 atatatatgt ccatgtacct agtgataact ccttaaatac ttccgaaaat gcataaggta
      601 cacttgagat tgccgaaatt ccagaacctg aatacagcat cagtactaca ctaaaactat
      661 tgatcacgat tgctgccatc aatgcaagct ctcctcttag tattggtttt tcattctcca
      721 tctttcatcc tccaagtcta cacgtaatta tgaatataag tttagtcctt atttacatat
      781 tttacaagtt agattttttt cataaatcta tagaagatat ctattaattc tctgtcaaga
      841 ttagattctg tacttttttc tgtaaatatg atatgattgc acttgtgtct gttcatgttc
      901 catgtcacct taaggtaaca tggatcttaa tgagtaaata tctattatat aagaacagat
      961 acaaattaat aaaagaaatt tgaaagaggt ttgatttatg ttagagctta tacaatcagt
     1021 taacagtgcc gtgaacaact ttatctgggg tgttccagcc atgatctgca tcattggtgt
     1081 cggattactg ttaacgatca aaacaaaatt tttacagatc cgtaaattcc cttatgcaat
     1141 gaaggaaacg cttggtcgtg tctttaagaa gcaagatgca tccgacggat ctatgactcc
     1201 atttcaggca gtctgtactg cactcgcttc cacagtcgga actggaaata ttgccggagt
     1261 tgccggtgcg atcgcaatcg gtggtcctgg tgctgttttc tggatgtgga tttctgctat
     1321 tcttggaatg tgtacgaaat tttccgaagt aacacttgca gttcacttca gagaacgtaa
     1381 ccaagaaggg gattatgttg gtggtcctat gtactatatc aagaatggtc ttagcaaaaa
     1441 ctggcatttt cttgctgtac tattctctgc tttcggagtt ttaacagtat tcggaactgg
     1501 aaatgctact caggtaaata cgatcacaac agcaatcaat tctgctttgt tgaatttcca
     1561 cgtgatcagt cagtcttctg ttggaactac gaatctgatc attggtatca ttgttgcaat
     1621 tcttgtggca ttgatcttac ttggcggaat caaaagaatc ggacaggtag ctgaaagatt
     1681 agtacctttt atggcattta tttatatctt ctttgcctta ggtgttgttg ttttaaatat
     1741 cgatcagctt cctgctgttt ttggttctat cattaacgga gctttccatc ctgcttctgt
     1801 gactggtggt attgttggaa gtttctttat gagtatgaaa aaaggtgtcg cacgaggcat
     1861 cttctcaaat gaagccggac ttggtacagg ttcgatcgct catgcctgtg ctgatacgaa
     1921 agagcctgta aagcagggac ttcttggtat ctttgaagta tttactgata cgatcttaat
     1981 ctgtacattg acagctttgg tcatcctctg cagtggaatt ccaattggat ttggtgcgga
     2041 tgctggtgct gaacttacga tcagtggatt tacttctact tatggtaact ggatctccat
     2101 ctttactgca gttgcgttat gttgctttgc attttcaacc attatcggat ggggattata
     2161 tggagctcgt tgtattgagt ttctattctc ttctaaggtt attaaacctt ttatgttagt
     2221 atattcgtta gttgcgatcc ttggtgctac tgtagacctt ggaatgcttt ggagtatcgc
     2281 agaaacattc aacggtctga tggcgattcc aaacttgatc gctttattcc ttctatctgg
     2341 aactgtggta aaattggtaa aagactattt tcatgagaaa tagttgcaca ctcttgcttt
     2401 tccacttcgt tttgcctggt aaagtgcctg atcggcgcgt ttatagagat ccatgtagca
     2461 gtccccgtct tgtggggctg tgctgattcc gatactgatg gagatattat ttcctttcaa
     2521 ttcgtcgat
//
LOCUS       DAOMUE010000295         2510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_11898_length_2510_cov_2.808961, whole genome shotgun sequence.
ACCESSION   DAOMUE010000295 DAOMUE010000000
VERSION     DAOMUE010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11898_length_2510_cov_2.808961"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLC71_10140"
     CDS             <1..505
                     /locus_tag="PLC71_10140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HRM03037.1"
                     /translation="IMSTIRTQKTKENLDFLTGLPTRNKGEQAIAQLMKHHSGCLVFM
                     DMDNLKTINDIYGHKAGDRVLKLLGNLLLEYSSECLVCRLGGDEFLLFMPDADQNSIT
                     EVITAIFKKFNTRKEQDPELHAASISAGLYMCNIGDSFDECYTKADKALYFVKQNGKS
                     DFSFHEK"
     gene            complement(562..1959)
                     /gene="mgtE"
                     /locus_tag="PLC71_10145"
     CDS             complement(562..1959)
                     /gene="mgtE"
                     /locus_tag="PLC71_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRM03038.1"
                     /translation="MKNARNQHRTDYAKELVKIVQTTKDREELKKKIAAYHERDIANA
                     IVESDKTVRKCIYDILDIADIAEIFSYIEEEPGKYLEEMPIEQAAKVVSNMDSDDAMD
                     LFEELNEEDKYKLLKRLDKEAKEDVQKLLSYEEDQIGSCMTNNYIVVRNDVTIREAMS
                     TLVKQAGEHDNIQTLYVIDENGIFAGAIDLKDLIIAREHDNLQDIISSSYPYVYEHEK
                     ISECMEILTDYEEDSIPVLTQDKKIAGILTSSDIVELVDDEMGDDYAKLAGLTEEEDL
                     NEPTIVSMKKRLPWLIALLFLGMAVSSVVGMFESIVAVLPIVICFQSLVLDMAGNVGT
                     QSLAVTIRVLMDENLSGKKKIALLFKEMKIGFLNGASLAIMALVFLGVYIHLFKHYAW
                     ISSFLISGCVGISLIVAMVISSLVGTIIPMFFHKINIDPAVASGPLITTINDLVAVIT
                     YYGLSGLVLIQLFHI"
BASE COUNT          767 a          525 c          369 g          849 t
ORIGIN      
        1 catcatgagc acgatccgta cacaaaaaac caaagaaaat ctcgactttt taactgggct
       61 tccaaccaga aataaaggtg agcaagccat tgcgcagttg atgaaacacc acagcggatg
      121 tcttgtcttt atggatatgg acaatttaaa aaccatcaat gacatctatg gccacaaagc
      181 tggtgaccgt gtattgaagc ttcttggtaa tctattatta gaatattcca gtgaatgtct
      241 tgtctgtcgt cttggcggag atgaattcct attatttatg ccagatgctg atcaaaatag
      301 tattacagaa gttataactg ctatttttaa gaaatttaat actcgaaagg aacaagatcc
      361 tgaacttcat gctgcatcca tttctgccgg actatatatg tgcaatattg gagattcttt
      421 tgacgaatgt tatacaaaag ctgataaagc actatatttc gtaaaacaaa atggaaaaag
      481 cgacttctcc tttcatgaaa aataaagcac taaacatata aagaagagag tggcaagaat
      541 ttacttttgc cactctcttc tttatatatg aaataactga atcaacacaa gaccagaaag
      601 tccataatat gtgataactg caactaaatc attgatagtt gtgatcaatg gaccagaagc
      661 cactgctggg tcaatgttga tcttatgaaa gaacattgga atgatcgttc ctacaaggct
      721 tgagatcacc atcgcaacga tcagtgaaat tcctacgcac cctgaaatca ggaatgaaga
      781 aatccatgca taatgtttaa ataaatggat gtacacccct aaaaatacga gtgccatgat
      841 tgctaggctc gctccattta agaaaccgat cttcatttct ttaaataaca aagcaatctt
      901 tttcttacct gacagatttt catccattaa tacgcggatt gttacagcta aagactgtgt
      961 tccaacattt cccgccatat caagtaccag ggattggaaa cagataacga ttggtaatac
     1021 cgcaacaata gattcaaaca ttccaacaac tgaggatact gccattccta agaataacaa
     1081 tgcgatcaac catggaagtc tcttcttcat actaactatc gtgggttcat ttaaatcttc
     1141 ctcttctgtt aaaccagcaa gttttgcata gtcatctccc atctcgtcat cgaccaattc
     1201 tacaatatcg gaagatgtta agattcctgc gattttctta tcttgtgtca gtactgggat
     1261 ggaatcttct tcgtaatctg ttaagatttc catacattca ctgatttttt catgttcata
     1321 tacataagga taagaactgc ttatgatgtc ctgtagatta tcatgttctc ttgcgatgat
     1381 caaatctttc agatcgatcg ctcctgcaaa aatcccattc tcatctatca cataaagtgt
     1441 ctggatatta tcatgttctc ctgcctgctt tacaagtgta ctcattgcct cacgaatcgt
     1501 tacatcattt cttactacga tataattatt cgtcatacaa ctaccgatct gatcttcctc
     1561 gtaagacagc aatttctgaa catcttcttt tgcttcttta tccagtctct ttaataattt
     1621 gtatttatct tcttcattta attcttcaaa cagatccatc gcatcatctg aatccatgtt
     1681 tgatacaact ttcgctgcct gttcaattgg catttcttcc agatattttc ctggttcctc
     1741 ttcgatataa gaaaaaatct ctgcaatatc tgcaatgtct aaaatgtcat agatacattt
     1801 tctaactgtt ttatctgatt ctacgatcgc atttgcaata tctctttcgt gataagctgc
     1861 gatctttttc tttaattctt ctctatcttt cgttgtctgt acgatcttga ccaattcttt
     1921 tgcataatct gttctatgct gatttcttgc atttttcatg atatcctcct tcttttccta
     1981 aatctggttg caaaacacca atatttaggc acaaaaatac accgcttcag attgaaattc
     2041 ctaaagcaga tcacatactc tgttgtgatg taaagaactt tctgtttcgc ggtgtatcaa
     2101 aaaggatatc ataatacaaa tgttgctatt catgtatctt cacagagagc agcattcgca
     2161 tcttatattt tcttttttga ccccgcatac aacttcgtcc tatacgatcg caactatcca
     2221 tgaacataca cctcctttca catctttact aatatctctc aacattcttt gttttcattc
     2281 tgggttatat tatcagattt ttctgatatc gtcaacctat tttactataa tttaacaatc
     2341 ttttccgccg cagtgtgatc ctgcccggag ggctttttat tgcatagggg atttcatcga
     2401 aattttctat gcaataaaaa aaacatcctt atctctaaga atgcttgaat cttcatatta
     2461 aatctatata taaaggatat accttcaaaa caacatacaa gaatctttct
//
LOCUS       DAOMUE010000296         2491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12014_length_2491_cov_1.622742, whole genome shotgun sequence.
ACCESSION   DAOMUE010000296 DAOMUE010000000
VERSION     DAOMUE010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12014_length_2491_cov_1.622742"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>461)
                     /locus_tag="PLC71_10150"
     CDS             complement(<1..>461)
                     /locus_tag="PLC71_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HRM03039.1"
                     /translation="HKPQAMGREMVRYAGSILKYSFSEIHKDKQATILTISKNKQISL
                     SHHLLKPLRDMREMECSLESLLKRKCEIGNEEDYMHVILTDEEQILDAIGKVRTVYPN
                     VMQISFKNRRHMMQYETIQMKENQIADQNPMELFEQFYKMQNHIDLDEKRA"
     assembly_gap    462..490
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<491..>756)
                     /locus_tag="PLC71_10155"
     CDS             complement(<491..>756)
                     /locus_tag="PLC71_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HRM03040.1"
                     /translation="IKEDGYNINFYMLPFVKPAYINHVLKIQTETYEECFKHLMEQVK
                     INEDETNILLAHQFVTVGKNSPELSDSETSSLGGIDNIDYRLFD"
     assembly_gap    757..766
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<767..1106)
                     /gene="sbcD"
                     /locus_tag="PLC71_10160"
     CDS             complement(<767..1106)
                     /gene="sbcD"
                     /locus_tag="PLC71_10160"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HRM03041.1"
                     /translation="MKILHLGDLHLGKRVNEISMIEDQKFILDQIVTLVKEEKIDVIL
                     LCGDIYDKAIPTIEAIHLLDEFLEELSDLKVKVLMISGNHDSSERLSFGRNLFKRSNL
                     YIASQFNQEIE"
     gene            complement(1177..2394)
                     /gene="spoIVB"
                     /locus_tag="PLC71_10165"
     CDS             complement(1177..2394)
                     /gene="spoIVB"
                     /locus_tag="PLC71_10165"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HRM03042.1"
                     /translation="MKKYTYRKFLILLFMINLIAITAVYIKYCNARLPDTIHINKGET
                     VSLCYDVPVSATIDHQTIRLNQPIVFKQCHMGQYEMKTNLFGAIPLKKIKVKVVDDQK
                     ITPSGEVIGVYVETDGLFVLDTDQFTGENSMEYAPSKNKLYRGDYIKKIDGKDIVSKK
                     QFIEKINQGQGESVILTVQRKAKMMQIKIKPERSKTDHMYKIGTGIRDNTQGIGTMTY
                     VKGTSFGGLGHGIYDMDTGTLLKIKGGLLLDPQIYSIKKGKSGTPGEIVGSIEYKEEN
                     ILGSIKKNTEKGIYGTIPKKQQKAYKIGYRQDIKPGKAYILSNVSGTMKQYEIQINKI
                     VPSDKDVLKSMEIEVTDKELLKLTNGIIQGMSGSPILQNGKLIGAVTHVFVNDPMKGY
                     AILMETMLYEMEN"
BASE COUNT          650 a          514 c          353 g          935 t
ORIGIN      
        1 gtgctctttt ttcatccaga tcaatatgat tttgcatttt ataaaactgt tcaaataatt
       61 ccattggatt ttgatctgcg atctggtttt ctttcatctg aatggtttca tattgcatca
      121 tatgacgacg gtttttaaag ctgatctgca taacattcgg ataaaccgta cgtactttcc
      181 cgatcgcatc taaaatctgc tcctcatccg tcagaatgac atgcatataa tcttcttcat
      241 ttccaatttc acattttctt tttaacaatg attctaacga acattccatt tccctcatat
      301 cacgtaatgg ttttagcaaa tgatgggata gggagatctg cttgttttta ctgatcgtaa
      361 ggatcgttgc ctgtttgtct ttgtgaattt ctgaaaaaga atattttaag atcgatccag
      421 cataccttac catttcacgt cccattgcct gcggcttgtg gnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn catcaaatag acggtaatca atattatcaa tccctccaag gctgcttgtt
      541 tctgaatctg acaattctgg agaattcttt ccaaccgtta caaactgatg cgccagcagg
      601 atattcgtct catcttcgtt gatcttcact tgttccatta gatgcttgaa gcattcttca
      661 taggtttctg tctgaatttt taatacatga ttgatatacg ctggctttac aaatggaagc
      721 atataaaaat taatattgta tccatcttcc ttgatcnnnn nnnnnntttc aatttcctga
      781 ttaaattgtg atgcaatata taaattagat cgtttaaata agtttcttcc aaatgacaat
      841 ctttccgaag aatcatgatt tccgctgatc atcaatactt ttactttaag atcactcagt
      901 tcttctaaaa attcatccag cagatgaatc gcctcgatcg tcggtattgc cttatcatag
      961 atatccccac ataataaaat aacatctatc ttttcttctt ttactaatgt aacgatctga
     1021 tccaatataa atttctgatc ttcaatcatg gatatctcat tcactctctt tccgagatgc
     1081 agatctccaa gatgcaaaat cttcatgtct cttaccctcc cttttttcta ttgtagcaaa
     1141 cttatatttt aaaaacagct tccatttccc tttgctctaa ttttccatct catagagcat
     1201 tgtttccata agaatagcat accctttcat cgggtcattt acaaaaacat gtgtaacagc
     1261 accaataagt ttcccattct gcaagattgg actgccactc attccctgta tgatcccgtt
     1321 ggtaagtttt aataattctt tgtctgtcac ttcaatctcc atacttttta acacatcctt
     1381 gtcggatggc acgatcttat tgatctggat ctcatattgt ttcatagtac cgcttacatt
     1441 ggagagaata taagctttcc ctggtttgat atcttgacga tatcctatct tgtatgcttt
     1501 ttgttgtttc tttggtattg ttccataaat tcctttttcc gtattttttt tgatggaacc
     1561 taaaatattc tcttccttgt attcaatact tccaacaatc tctcccggcg ttcctgattt
     1621 tcctttttta atggaataaa tctgtggatc cagcaaaaga ccacctttga tctttaaaag
     1681 tgtgcctgta tccatatcat aaataccgtg tcccaaccct ccaaatgacg taccttttac
     1741 ataagtcatc gtaccgattc cctgcgtgtt atccctgatc cctgttccaa ttttatacat
     1801 gtgatccgtc tttgaacgtt ctggcttgat cttgatctgc atcatctttg cctttctttg
     1861 gactgttaat atcacagatt ctccttgacc ttgattgatt ttttcgatga attgtttttt
     1921 tgatacaata tcttttccat caatcttttt gatatagtct ccacgatata atttgttttt
     1981 acttggtgca tattccatgc tgttttctcc ggtaaactga tccgtatcaa gcacaaacaa
     2041 tccatctgtt tctacataga caccgatcac ttcaccgctt ggcgtgatct tttggtcatc
     2101 tacgaccttc actttaatct tctttaatgg aatcgcgcca aataaattgg ttttcatttc
     2161 atactgaccc atgtgacatt gtttgaatac aatgggctga tttaatcgta tcgtttgatg
     2221 atcgatcgtt gcactgactg gtacatcata acaaagagag actgtttcgc ccttgttgat
     2281 atgaatcgta tctggaagtc ttgcattgca gtattttata taaactgcag ttattgcgat
     2341 taaattgatc ataaaaagta gaattaaaaa tttgcggtaa gtgtattttt tcatattttt
     2401 cctcctaaaa aaggacatat cgtcattgat atatcctttt tattatatga agattaccgc
     2461 ttacatactc agtataattt tgtttcatct g
//
LOCUS       DAOMUE010000297         2472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12114_length_2472_cov_1.705006, whole genome shotgun sequence.
ACCESSION   DAOMUE010000297 DAOMUE010000000
VERSION     DAOMUE010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12114_length_2472_cov_1.705006"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1429)
                     /locus_tag="PLC71_10170"
     CDS             complement(<1..>1429)
                     /locus_tag="PLC71_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03043.1"
                     /translation="EFLIPIQVKGKEYNILPDSDSISYPVDINDLKNYLKNGGVIFFV
                     DAISKTEYNDKMYAKILLPVDIVHIMKGKEEQGSITVHLNHVKTVRELEELCVLFKEN
                     RPKQAMVGVGKSIPDYDNISNFTISTLPTPYMNPAIAIAKSPFKYVYTKKDGMEIPVL
                     LTDLVTATESEWLIKIDDKIEQRYPVRHIYELNQTTTRISEIIEFVLTEKSQKTTIRC
                     VEATNYNFETVFQASKIIIAVSDAEEVMIENISLGKNFLGEIQTAFTEGFLAYHKRIV
                     QLGETLAELNIKRSLFKTKEILDAEPTIFFLKKGLVEMKVLSLPFQADENCGLYKQII
                     GRRKLLLEYEKIQEDGYIIRNYLKDGKRIAIFADIPDGKTQKISRWFALRDEDIFQMI
                     FDENDLLEEMYNIGEEETDQLLGLILDFLKSYDKERDKNLLLLAEKLINILEKYSMDS
                     TILTINRLQIIARKRELELEERKTLA"
     assembly_gap    1430..1439
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1683..2066)
                     /locus_tag="PLC71_10175"
     CDS             complement(1683..2066)
                     /locus_tag="PLC71_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6262 family protein"
                     /protein_id="HRM03044.1"
                     /translation="MSKYDKMLEVNHKQSVEKIQRAKLTIQEMIEEEDKVTVPKLMQK
                     TGLSRGFFYKNPEVRKAVDRALQLQAGMVDKRRKILDMAMDNRILQLEQQVAKLKREN
                     ETLRKESETMRKALNKRDLNLIKNF"
     gene            complement(2053..>2472)
                     /locus_tag="PLC71_10180"
     CDS             complement(2053..>2472)
                     /locus_tag="PLC71_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRM03045.1"
                     /translation="SAELELLIEKAIEYSKKRYGDTVYIFVDEKNSKRPMQYNTVQNK
                     VMDLIQKKDLRDDHGELFGFGTHMFRHVYGIRLTELHLDDWTIAKLLGHTSVKNVKFY
                     RKMSLQIIADETREIRAEMSRMIRANLAGWGKEYEQI"
BASE COUNT          642 a          540 c          332 g          948 t
ORIGIN      
        1 gtgccaatgt tttcctttct tcaagctcta gctccctttt acgggcaata atttgaagtc
       61 tatttattgt aagaatggtt gaatccatcg aatatttctc taagatattt attaattttt
      121 cagccaataa caataaattt ttatctcttt ccttatcata agattttaga aaatccaata
      181 ttagtcccaa aagttgatcc gtttcttctt caccgatgtt gtacatttct tccaacagat
      241 cattttcatc aaatatcatc tgaaatatat cctcatctcg taacgcaaac catctgctga
      301 ttttctgtgt ttttccatct ggtatatcgg caaagatagc aatccgttta ccatccttca
      361 aataattacg aattatatat ccatcttctt gaatcttttc atattctaac agcaatttcc
      421 tacgtccaat tatttgtttg taaagaccac aattttcatc tgcttgaaat ggtaacgata
      481 aaactttcat ttccactaat ccctttttca gaaaaaatat ggtaggttct gcatctaaaa
      541 tttctttcgt tttaaataaa cttcttttaa tatttagttc tgcaagtgtt tctcctaatt
      601 gaacaatccg tttatgatat gcaagaaatc cttctgtaaa agctgtttgt atttccccta
      661 agaaattttt tcccagactt atattttcaa tcattacttc ctctgcatca gaaacagcta
      721 ttataatttt tgatgcttga aaaactgttt caaaattata gttagttgcc tcaacacaac
      781 gaatggttgt tttttggctt ttttctgtta atacaaattc gatgatctca ctaatccttg
      841 tagtcgtttg atttaattca taaatatgtc ggacaggata tctttgttct attttatcat
      901 cgatttttat taaccactca ctctcggtcg cagtaactaa atctgtcaat aaaactggta
      961 tctccatccc atccttcttg gtatacacat acttaaaagg acttttcgca attgcgattg
     1021 caggattcat atatggagtt ggcaatgttg atatagtaaa attagatata ttatcataat
     1081 cgggaattga ttttcccacc cctaccattg cctgtttagg tctattttct ttaaaaagaa
     1141 cacacagctc ttctaattct cggacagttt ttacatggtt taaatgtacc gtaatgctcc
     1201 cctgttcttc ttttcctttc attatatgga caatatctac cggaagtaag atttttgcat
     1261 acattttgtc attatattcc gtttttgaaa ttgcatcaac gaaaaatatt actccgccat
     1321 tctttaaata atttttcaaa tcatttatat ccactggata acttatgcta tccgaatccg
     1381 gaagaatatt atactctttt ccctttactt gaataggaat aagaaattcn nnnnnnnnnt
     1441 atgaatatct gactcatcca aatattcaga tctagcacac attctttgta gtgttgttat
     1501 agaaaatgtt tcttgactac tcatttatct gaatctccat gtagttgttc atttctgaaa
     1561 gttgattgtt ttctattaca ttatactata tagcacacta cgtttctaga tattttatct
     1621 ccacaaccac cagcaggcgc gttcttcacg cccctgccag tcgctgattg ttttcttctt
     1681 cattaaaaat tcttgatcag attcaagtct cttttattca atgctttccg cattgtttca
     1741 ctttcttttc ttaatgtctc gttctctctt ttcagtttcg ctacctgttg ctccagctgc
     1801 aggatacggt tgtccattgc catatctaaa atctttcttc tcttatctac catgccagcc
     1861 tgtagctgca aggcacgatc tactgccttt cgcacttctg ggttcttata aaaaaatcct
     1921 ctggacagtc cggtcttttg catcagttta ggaacggtca ctttatcctc ttcttcgatc
     1981 atttcctgga tcgtcagttt tgccctctga atcttctcca cgctctgctt atggtttact
     2041 tccagcatct tgtcatattt gctcatactc tttcccccat cctgccagat tcgcccggat
     2101 catccggctc atctctgctc tgatttctct tgtttcatct gcgatgatct ggaggctcat
     2161 cttccggtaa aatttcacat tctttacgga tgtatgcccc agtagttttg cgatggtcca
     2221 gtcatccaga tgcagttctg ttaagcggat accatataca tggcggaaca tatgcgttcc
     2281 aaatccgaat agttctccat ggtcgtcccg caaatctttt ttctggatca gatccatcac
     2341 cttgttttga acggtattat actgcatagg tctcttacta tttttctcat ccacaaaaat
     2401 gtaaaccgta tctccatacc ttttcttgct atactctatt gctttttcaa tcagcaattc
     2461 cagttctgca ct
//
LOCUS       DAOMUE010000298         2467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12145_length_2467_cov_1.951907, whole genome shotgun sequence.
ACCESSION   DAOMUE010000298 DAOMUE010000000
VERSION     DAOMUE010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12145_length_2467_cov_1.951907"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /gene="truA"
                     /locus_tag="PLC71_10185"
     CDS             complement(<1..642)
                     /gene="truA"
                     /locus_tag="PLC71_10185"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRM03046.1"
                     /translation="MKRNIRLTIEYDGSRYQGWQRLGGDSNANTIQGKLESVLSKMTG
                     EDVNINGSGRTDAGVHAYGQVANFHTDCMMSVNEIKNYLTKYLPSDIGIVEVSEASER
                     FHSRLNAKEKTYLYRIAIPGVSNVFERKYLWYFPETLDIKKMEEAASLLIGTHDFKGF
                     SSIKKTKKSTIRTIYDIKIKKTKKEIQITYTGNGFLHNMVRILTGTLVEVGCGK"
     gene            811..>1199
                     /locus_tag="PLC71_10190"
     CDS             811..>1199
                     /locus_tag="PLC71_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRM03047.1"
                     /translation="MEINQMLKRQEHFFQSQKTKKYEFRKKALLRLECAIKQHEEDME
                     HALYKDLGKSSFESYMAEIGMVKSELSYAKKHLKKWMKTEQVKTPLAQFPSQSYKIKE
                     PLGKVLIIAPWNYPILLSLQPLIGAIAA"
     assembly_gap    1200..1209
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1210..2139
                     /locus_tag="PLC71_10195"
     CDS             <1210..2139
                     /locus_tag="PLC71_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRM03048.1"
                     /translation="LKKMIGEVFPSHYVAVINGGVEISEKLLEQSFDHIFYTGGERVG
                     KIVMEKASRHLTPVTLELGGKSPCIVEESANIKLAAKRIVFGKFLNSGQTCVAPDYIF
                     VDKKAESELIFYLKYWINKMIGEHPLSNKDYSSMINPRHYQRIMELMKHEKIVEGGYG
                     DIRLRKITPTILVNVKEESTVMQEEIFGPLLPIMTYDKLEDAVSYIRDHNKPLALYLF
                     TENDKVEQDVLSQLSFGGGCINDTIIHLATPYLGFGGVGNSGMGSYHGKKSFDTFTHE
                     KSIIKKSDKIDIPVRYMPYNKVKEQLMKFFLKL"
     gene            2158..2412
                     /locus_tag="PLC71_10200"
     CDS             2158..2412
                     /locus_tag="PLC71_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03049.1"
                     /translation="MEFTNVLPGVKLVKQDEAGNEEELFVSQNDHVIVKTLNGREIKG
                     IFMQIEFARCLEEDDIVHVHKDNGENEGIPFDTIDDIIKG"
BASE COUNT          875 a          376 c          495 g          711 t
ORIGIN      
        1 ttttccacat ccaacttcca ctaaagttcc agtaagaatc cggaccatgt tatgaaggaa
       61 gccatttcct gtataagtaa tctggatttc ttttttcgtc ttcttgatct tgatatcata
      121 aatcgtacga atcgtagatt tttttgtctt tttaatagaa gaaaatcctt taaaatcatg
      181 agttccgatt agtaatgatg cagcttcttc catctttttg atatccaaag tttccgggaa
      241 ataccaaagg tatttacgtt caaaaacatt cgatacccca ggaatcgcaa tacggtaaag
      301 gtaggttttc tccttggcat ttaaacggct gtgaaaacgt tccgaagctt cagaaacttc
      361 cacaataccg atatcagatg gaagatattt ggtaagatag tttttgatct cattaacaga
      421 catcatacag tctgtatgga aattggcaac ctgaccataa gcatgaactc cggcatcagt
      481 acggccagat ccattgatat tgacatcttc tccagtcatc tttgataaaa cagactctaa
      541 ttttccttga atcgtattcg cattggaatc accgccgagc ctctgccagc cctgatagcg
      601 ggaaccatca tattcaattg ttaatctgat attacgtttc atgtatgtat aaatcctttc
      661 attgtaaaga tatggttgtt gtaaaacaaa tttattatag cgaatttctt aaaaagtggc
      721 aataaataaa aaagagaggc ggtagtttac tgtatatggt aaaattaaga taaaacgcgg
      781 tgaaggtaga gaaagaaagg ataggttatc atggaaatca atcaaatgtt aaaaagacaa
      841 gaacattttt ttcaaagcca gaagacaaag aaatacgaat ttcgaaagaa agcattattg
      901 cgcttggaat gtgcaatcaa acaacatgaa gaagatatgg aacatgcatt atataaagat
      961 ttagggaaat catcttttga gtcctatatg gcagagattg gcatggtaaa aagtgaactt
     1021 tcttatgcaa agaagcatct aaaaaaatgg atgaaaacag agcaggtaaa aacaccgctt
     1081 gcccagtttc catcccaaag ttataagatc aaagaaccac tcggaaaggt attgatcata
     1141 gccccatgga attatccaat tcttttatcc ttacagccgt tgatcggagc gatcgcagcn
     1201 nnnnnnnnnt taaagaagat gattggagaa gttttccctt ctcattacgt tgctgtgatc
     1261 aatggaggag tagaaatcag tgaaaaactt ctggaacagt catttgatca tattttctat
     1321 actggtggag aacgagttgg aaagatcgtg atggaaaaag catcgagaca tttaacacca
     1381 gtgactttgg aattaggtgg aaaaagtcca tgcattgtag aagaaagtgc caatattaag
     1441 ttggcagcaa aacgaattgt ttttggaaaa tttttaaaca gtgggcagac ctgtgttgcg
     1501 ccagattaca ttttcgtaga taaaaaagca gaaagtgaac tgatcttcta cttaaaatac
     1561 tggatcaata aaatgatcgg ggaacatcct ttgtcgaata aagattattc atctatgata
     1621 aatccgagac attatcagcg cattatggaa ttgatgaaac atgaaaagat cgttgaaggc
     1681 ggttatggag atataagact tcgaaagatc accccaacga tacttgtgaa tgtaaaagaa
     1741 gaatccacgg tgatgcagga agagattttt ggtccattac ttcctataat gacatatgac
     1801 aaattagaag atgcagtttc gtatatcaga gatcataaca aacctttggc attgtattta
     1861 tttacagaaa atgataaagt agaacaagat gtactttcac agttgtcttt tggcggtggc
     1921 tgtatcaatg atacgatcat ccaccttgca actccatatc taggatttgg aggagttggg
     1981 aatagcggaa tggggtctta tcatgggaag aaaagttttg atacatttac gcatgaaaag
     2041 agtatcataa agaaatcaga taagattgat attccagtcc gttatatgcc atataacaaa
     2101 gtcaaagagc aattgatgaa attttttctg aaattataag ataaaggagc aacttttatg
     2161 gaatttacaa atgtattacc aggagtgaaa ttggtaaaac aagatgaagc tgggaatgag
     2221 gaagaacttt ttgtttcaca aaatgatcat gtgatcgtaa agacattaaa tggaagagag
     2281 atcaaaggta tttttatgca gattgaattt gcaagatgct tagaagaaga tgatattgta
     2341 catgtgcata aagacaatgg ggaaaatgaa ggaatcccgt ttgacacgat cgatgatatc
     2401 attaagggtt aagaaagaaa aagaggatta aaatttatat ggaaaatcag aatcagaatg
     2461 tcagtgc
//
LOCUS       DAOMUE010000299         2461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12184_length_2461_cov_3.389443, whole genome shotgun sequence.
ACCESSION   DAOMUE010000299 DAOMUE010000000
VERSION     DAOMUE010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12184_length_2461_cov_3.389443"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..836)
                     /locus_tag="PLC71_10205"
     CDS             complement(42..836)
                     /locus_tag="PLC71_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM03050.1"
                     /translation="MLEIKNVHKTFNKGTINEKKALNGVNLHLDPGDFVTIIGGNGAG
                     KSTTLNMVAGVYPIDQGTIILDGKDISDLPEYKRAYMLGRVFQDPMMGTAAGMQIEEN
                     MAMANRRGKKRGLSWGITKKEKEMFKEKLQMLDLGLEDRMTSKVGLLSGGQRQALTLL
                     MATLKKPSLLLLDEHTAALDPKTAKKVLDITEKIVARDNLTTMMVTHNMKDAINIGNR
                     LIMMSDGKIIYDVKGEEKKKLKVADLLQKFEEASGGEFANDRMLLS"
     gene            complement(853..1746)
                     /locus_tag="PLC71_10210"
     CDS             complement(853..1746)
                     /locus_tag="PLC71_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM03051.1"
                     /translation="MALITNLYSALAQGTLWGIMALGVYITFRILDIADLSCDGTFAL
                     GGCISAILITRGVNPFVTLLFALVGGMLAGLVTGWLHTKLMIPAILAGILTMIALWSV
                     NIRVMGGPNVSLLGEDTAFTKVMDLLGVDQTLATLVVGVIIGAIVIAALYWFFGTEIG
                     CALRATGNNEFMVRALGGNTDTSKVLGLVISNGLISVSGALVAQNQGYGDIKMGTGSI
                     VIGLASIVIGEVIFGKRFNFAYILASLIGGAMVYRMIISLVLHFGLSTDDMKLFTAII
                     VALALGIPALKSKYSHRRTPA"
     gene            complement(1844..>2461)
                     /locus_tag="PLC71_10215"
     CDS             complement(1844..>2461)
                     /locus_tag="PLC71_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRM03052.1"
                     /translation="VKSNKKPGTNVSGTSDLTPVAKQMNLLKELIPNAKKVAMLYCSA
                     EANSKFQVGLAKKEAKKLGLTTVDATVSESSEIRQVVESLKGKVDAIYSPTDNMIAAG
                     INTVSMVANEAKIPFIVAEEGMCTGGGLATYGVNYYKLGQQTAKQAVKILEGKAEPKD
                     MPIEYQENADLIINKDTVKTLGIKIPEKLKKEAKMVTTQKKTDKE"
BASE COUNT          611 a          552 c          403 g          895 t
ORIGIN      
        1 tatactcgaa atctacagtt ggccaactac aaattaaaat tttaagacaa caacattcta
       61 tcgtttgcga attctccgcc gctggcttct tcgaatttct gcaataaatc tgctacctta
      121 agcttcttct tttcttcccc tttcacatca taaatgatct ttccatcact catcatgatc
      181 aggcggtttc cgatattgat cgcatctttc atattatgtg ttaccatcat tgttgtaaga
      241 ttatctctgg ctacgatctt ctctgtgatg tctagtactt tcttcgcagt ctttggatca
      301 agggctgctg tgtgttcgtc taataatagc aggcttggtt tcttcagtgt tgccataagc
      361 agtgtcaatg cctgtctctg acctccggat aatagtccta ctttggatgt cattcgatct
      421 tctagtccta agtcgagcat ttgtaatttt tctttaaaca tttctttttc tttttttgtg
      481 attccccagc ttaggcctct tttctttcct ctacggtttg ccattgccat gttttcttca
      541 atctgcatac ctgctgccgt tcccatcatt gggtcctgga atactcttcc taacatgtag
      601 gcacgtttgt attctggaag atctgagata tccttgccat ctagaatgat cgttccttga
      661 tcgatcggat agactcctgc taccatgttg agagttgtag attttccggc cccatttcct
      721 ccgatgatcg ttacgaagtc cccaggatct aagtgaagat tgactccatt taatgctttt
      781 ttctcattga tcgttccttt attaaatgtt ttatgtacat ttttaatttc taacataact
      841 ttgcccctct ttctatgctg gtgtcctgcg gtgtgaatat tttgatttga gagcaggaat
      901 accgagtgct aatgctacga tgatcgctgt gaataatttc atatcatcag tacttaatcc
      961 gaaatgtaaa acaagtgaga tgatcatacg atataccatt gctcctccga tcagtgatgc
     1021 aaggatataa gcaaagttaa aacgttttcc aaagataact tctccgatta caatggatgc
     1081 aagaccaatt acaatagatc ctgttcccat tttgatatct ccgtatcctt ggttctgggc
     1141 tactaaagct ccagatactg agatcagtcc attagaaatc accagtccta aaactttgga
     1201 tgtatccgta tttcccccta aagcacgtac cataaattca ttgtttcctg ttgccctaag
     1261 tgcacatccg atctctgttc caaaaaacca gtacagagct gcgatcacaa tggctccaat
     1321 aatgactcca actaccaatg ttgccagtgt ctgatctact cctaacagat ccattacctt
     1381 tgtaaatgca gtgtcttctc caagtaatga aacatttggt cctcccataa ctctgatatt
     1441 aacggaccat aatgcgatca ttgttaaaat acctgctaag atcgcaggga tcataagttt
     1501 cgtgtgcagc cagcctgtaa caagtcctgc taacattcct cctacaagtg caaataataa
     1561 tgttacaaat ggattgactc cccttgtaat aaggattgca ctgatacatc caccgagtgc
     1621 aaatgttcca tcgcaggata agtctgcgat atctaagatc ctaaatgtaa tataaactcc
     1681 cagtgccatg attccccata atgttccttg ggctaatgca ctataaagat ttgtaattaa
     1741 tgccattttc tacctctttt ctaaatgcac agaagtgcct cccgcaaatc ggaagacact
     1801 tcctgatctt cttgtaatct tatatgcaag aaaatcttat ttattattct ttatctgtct
     1861 ttttctgtgt tgttaccatc ttagcttctt tttttaactt ctctggaatc ttaattccta
     1921 atgtctttac tgtgtcttta ttgatgatca gatccgcatt ttcctgatat tcaattggca
     1981 tatcttttgg ttcagctttt ccttctaaga tctttactgc ctgctttgct gtctgctgtc
     2041 ctaacttgta gtagttaact ccgtatgttg caagtcctcc acctgtacac attccttctt
     2101 cagctacgat aaatggaatc ttagcttcat ttgctaccat ggatactgta ttgattcctg
     2161 ctgcgatcat gttgtctgtt ggtgaataga ttgcatcaac tttacctttt aaggattcta
     2221 caacctgacg gatctcactg gattctgata ctgttgcatc gactgttgtt aatcctaatt
     2281 tctttgcttc tttcttcgca agtcctacct ggaatttgga gtttgcttct gcagaacaat
     2341 ataacattgc aactttcttt gcatttggaa ttaattcttt taatagattc atctgttttg
     2401 ctactggtgt taaatctgat gttccagata cgtttgttcc tggtttttta tttgatttca
     2461 c
//
LOCUS       DAOMUE010000300         2458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12210_length_2458_cov_2.228881, whole genome shotgun sequence.
ACCESSION   DAOMUE010000300 DAOMUE010000000
VERSION     DAOMUE010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12210_length_2458_cov_2.228881"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PLC71_10220"
     CDS             <1..705
                     /locus_tag="PLC71_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HRM03053.1"
                     /translation="IHFLLSYDLFLYNRMRGTTLGINEEFVAAPKLDDLECAYSSIEG
                     MLNAKLSEDYVTVCAVFDNEEVGSGTKQGAGSTFFPEVLKRISYLCGKNEEEYYMAVA
                     DSFMLSADNAHAVHPNYQDKTDPTNRPYINEGIVLKYNASQKYTTDANSAALVKYLCK
                     KEKIKCQVFFNRSDMLGGSTLGNILTGQVSIKAADIGLPQLSMHSTYETAGCKDLDDM
                     ITLMGSFYELGKEVQI"
     gene            705..1373
                     /locus_tag="PLC71_10225"
     CDS             705..1373
                     /locus_tag="PLC71_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HRM03054.1"
                     /translation="MEEKRLQMSESFFLTAILAIVGGFLDSYSYLMRGHVFANAQTGN
                     IVLFGVNLQKRDFTQAFYYFVPILAFAVGVILVEIIKYFYKEEHKIHWRQRIVALEFV
                     LITVVGFIPLGQYDVVANVLISFVCSMQVETFRRVHGHPFASTMCTGNLRSGTEALCQ
                     YFKTGDKTLKQKSLRYYGIITFFIIGAVIGGFLTGRAGGSSIFICSILLAIATILMAK
                     EFRI"
     gene            complement(1437..1979)
                     /locus_tag="PLC71_10230"
     CDS             complement(1437..1979)
                     /locus_tag="PLC71_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM03055.1"
                     /translation="MFEKAVIYATNAHNGQTRKGTNLPFIIHPMEVAAIVAAMTLDQD
                     MLCAAVLHDVVEDCDGISIEDIRREFGDIVASYVYQESEDKSKTWVERKGHTIDFLKN
                     RASRNAKIIALGDKLANIRALYRDYNILGEELWERFNMKDKKMQSWYYRGMIEGFADL
                     SEFHEYEEYCQLVNEVFPEE"
     gene            2214..>2458
                     /locus_tag="PLC71_10235"
     CDS             2214..>2458
                     /locus_tag="PLC71_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRM03056.1"
                     /translation="MTDSNFTIGFIGLGLIGGSIAKGIRRVFPNYTLIGMDENPDTIK
                     SALEDGIIDSIATDCKTDFAECNYVFLCAPVQYNVMYL"
BASE COUNT          786 a          407 c          517 g          748 t
ORIGIN      
        1 attcattttc tattatctta tgatttgttt ttatataacc gaatgagagg aacaacacta
       61 ggaatcaacg aagaatttgt tgcagcacca aaattagatg acctcgaatg tgcatattca
      121 tccatcgaag gaatgttaaa tgcaaagctt tccgaagatt atgtcacagt gtgtgcggtc
      181 tttgataatg aagaagtcgg aagcggtaca aaacaggggg ctggatcaac atttttccca
      241 gaggtattaa aaaggatcag ttatctttgt ggaaaaaatg aagaagaata ttatatggcg
      301 gtggcagaca gctttatgtt atctgcagat aacgctcatg cagttcatcc gaactatcag
      361 gataagacag accctacgaa ccgtccgtat atcaatgaag gaattgtatt aaaatacaat
      421 gcatcacaaa aatatacaac cgatgcgaat tcagcggcac tcgtaaaata tctgtgtaaa
      481 aaagaaaaga ttaagtgtca ggtattcttt aaccgttcgg atatgttagg cggttcaaca
      541 cttggtaata tcctgactgg acaggtgtcg atcaaggcag cagatatcgg acttccacag
      601 ctatccatgc attcaacata cgaaactgcc ggatgcaaag atttagatga tatgatcaca
      661 ctgatgggat ctttttatga gcttggaaag gaagtgcaga tttaatggaa gaaaaacgtt
      721 tgcagatgtc agagagtttt ttcttaacag caattcttgc aatcgtagga ggctttcttg
      781 attcctacag ttatttaatg cgtggacatg tatttgcaaa tgcacagaca ggaaatatcg
      841 tattgtttgg tgtgaatctt caaaagcggg attttacaca ggcattttac tattttgttc
      901 caattcttgc atttgctgtg ggcgtaattc ttgttgaaat cataaagtat ttttataagg
      961 aagaacataa gattcattgg agacagagaa ttgtggcact tgagtttgtg ctgataacgg
     1021 ttgttggatt tattccactg ggacaatatg atgttgtggc gaatgtactg atttcttttg
     1081 tatgttctat gcaggtggag acatttcgaa gggttcatgg gcatccattt gccagtacga
     1141 tgtgtactgg taatttaaga agtggaacag aagcactgtg ccagtacttt aaaacaggag
     1201 ataagacatt aaaacaaaaa agtttacgat attatggaat tattactttt ttcattattg
     1261 gagcagtgat cggaggattt ttaaccggtc gtgcaggagg aagttctatc tttatctgta
     1321 gtattttgct agcgatcgca acgatcttga tggcaaaaga gttcagaatc tgataaaaag
     1381 actgatttta caaacattta acaatgtttg cgaaatcagt ctttttttct tttgtgttac
     1441 tcttcaggga aaacttcatt tacaagttga cagtattcct catactcatg aaattctgaa
     1501 agatcagcaa aaccttcgat cattccacga taataccaac tctgcatctt cttatccttc
     1561 atattgaatc tctcccataa ctcttcgcca aggatgttat aatcccgata aagtgcacgg
     1621 atgttagcaa gcttgtcacc aagggcgatg atcttagcgt tgcgggatgc acggttcttt
     1681 aagaaatcga tcgtatggcc ttttctctca acccatgtct tactcttatc ttcactttcc
     1741 tgatagacat aagaagcaac gatatcaccg aattcacgac ggatgtcctc tatcgagatt
     1801 ccgtcacaat cttcaacaac atcatgaagg acagcggcac aaagcatatc ctgatcgaga
     1861 gtcatagcgg caacgatggc agcaacttcc atagggtgga tgataaaagg aagattggtg
     1921 ccttttcttg tctgaccgtt gtgggcattt gttgcgtaga taacggcttt ttcaaacatt
     1981 ggaaaactcc tttattaaaa taagttagtg taaaataatt gttaagataa tcataaagca
     2041 tttttttttg gtattcaagt aggaaatcca agaaaataga gaggaaaata ctttgtcact
     2101 ttaaagcgtg tcagtgttga ctttttaaat acaggaagtt aaaataaaaa ctaagtgtga
     2161 tcaaagaatg agaagatttt gtgatcaccg caagaaaaaa ggagagtaaa acaatgacag
     2221 acagcaattt tacgatcgga tttatcggtt taggattgat cggtggatcc attgcgaagg
     2281 gaataagaag agtttttcca aactatacac tcattggaat ggatgaaaac ccggatacga
     2341 tcaaaagtgc attggaggat ggaatcattg attcaattgc tacagactgc aaaacagatt
     2401 ttgcggaatg caactatgta tttttgtgtg caccggttca atataatgta atgtatct
//
LOCUS       DAOMUE010000301         2446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12283_length_2446_cov_1.725220, whole genome shotgun sequence.
ACCESSION   DAOMUE010000301 DAOMUE010000000
VERSION     DAOMUE010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12283_length_2446_cov_1.725220"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PLC71_10240"
     CDS             <1..201
                     /locus_tag="PLC71_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRM03057.1"
                     /translation="SYSYLSRKIDFGTREQKLLKKVLNASLQRMIKEPIQELKHLETR
                     QEQADYKKMVEQLFGIETKKGK"
     gene            205..>1659
                     /gene="hemC"
                     /locus_tag="PLC71_10245"
     CDS             205..>1659
                     /gene="hemC"
                     /locus_tag="PLC71_10245"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRM03058.1"
                     /translation="MKYIIGSRGSRLALIQTKYVQEKLAKAYPEHTFEIQIIKTKGDK
                     IQNKPLDKIGGKGLFVKEIEEKIISGEIHMGVHSMKDMPSTPAKGLVFTKVWKREDPR
                     DVLILRTAKHLSELREGAVIATGSKRRAFQLLKLRPDLQIINIRGNVDTRLRKMEEQQ
                     LDGIVLAAAGLKRLGMEDVITQYLAPEDMISAPAQGALALEVREDQKELQKMLDVFCD
                     EETMNEVCAERNFLEQMGGSCHTPAGARCQKTDQGYELYAMFGNEDGSKQAYTKVYGT
                     CPEILAQTAVKNIKKQLAGIVYLIGAGPGDPGLITVKGKEILKKADCVIYDRLIPQEL
                     LDETKEGCEHIYVGKENHHHTMKQEQINELLAQKALQYDIVVRLKGGDPFVFGRGGEE
                     AIYLADHGIYCEVVPGISSCIAAAELAGIPVTHRGISKGFRVVTAHDRRNQLSDLNFA
                     SMAKSEETLVFLMGLSKLEEITTNLLKNGMDANTL"
     assembly_gap    1660..1669
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1670..2437
                     /locus_tag="PLC71_10250"
     CDS             <1670..2437
                     /locus_tag="PLC71_10250"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRM03059.1"
                     /translation="VAVVSSAASTKQQTCEATLNTIHEKVKQEKLSSPAVIVVGEVVK
                     LQKQIQKPEDTTCHLVTKIGDQPSKLAQILKDHGYKIKELQTGEISYRYDRISKEELA
                     KVTYLIFTSRYGVHGFMKQMKSSNLDLRDLFDKKIVVVGKKTKDVLMQYGIIADLMPE
                     EAGEINLSELMKQEVDAKDVVWYCKGISGGEKLKKALTPICQVTEKVMYENNRKILSE
                     IPEMKDIRSVSFTCASSARRFFDQIGEEKQQWIENIP"
BASE COUNT          921 a          365 c          546 g          604 t
ORIGIN      
        1 agttattcat atttatcaag aaaaatagat tttggaacaa gagagcagaa acttctaaag
       61 aaggtgttaa atgcatcctt gcagcgcatg atcaaagaac cgattcagga attaaaacat
      121 ttagaaacaa gacaggaaca agcagattat aagaagatgg tagaacagct ttttgggata
      181 gagacaaaga aaggaaaatg acctatgaaa tatatcatcg gatcaagagg atcacgcctt
      241 gcactgatcc agacaaaata tgttcaggaa aaattagcga aagcttatcc agaacacact
      301 tttgaaatcc agatcattaa aacaaaaggt gataagattc agaataaacc attagataaa
      361 attggtggaa aaggtctgtt tgtcaaagag attgaagaaa agatcatttc tggggaaatc
      421 catatgggag ttcacagcat gaaagacatg ccgtcaaccc cggcaaaagg ccttgttttt
      481 acaaaagtat ggaaaaggga agatccaaga gatgtgttga tccttcgaac agcgaaacat
      541 ctgtctgaat tacgagaagg tgctgtgata gcaacaggaa gcaaaagaag agcatttcag
      601 ttattaaagt taagaccaga ccttcagatt ataaatattc gagggaatgt tgatacgaga
      661 cttcgaaaaa tggaggaaca acaattagat ggaatcgttc ttgcggcagc aggtcttaaa
      721 agactaggaa tggaagatgt gatcacgcaa tatctggcgc cagaagacat gatctcagca
      781 ccagcacagg gtgctctggc tttggaagta agagaagatc agaaagaatt gcaaaagatg
      841 ttggatgtat tttgtgatga agaaacaatg aacgaagtat gtgcagaacg aaatttctta
      901 gaacaaatgg gaggcagttg tcatactccg gcaggtgcca gatgtcaaaa gacagatcaa
      961 gggtacgaac tatatgctat gtttggaaat gaagatggaa gtaaacaggc atatacgaaa
     1021 gtttatggaa catgccctga gatcttagca caaacggcag taaaaaatat caaaaaacag
     1081 ctggcaggaa tcgtatattt aattggtgca ggaccgggag atccaggact tatcacggtt
     1141 aaaggaaaag aaatcttaaa aaaggcggat tgtgtcattt atgacagatt gatcccacaa
     1201 gaattattag atgaaacaaa agaaggatgc gaacatatct atgttggaaa agaaaatcat
     1261 catcatacga tgaaacaaga acagatcaat gaattattag cacaaaaagc attacaatat
     1321 gatatcgtag tgcgtttaaa aggtggggat ccatttgtct ttggaagagg tggagaagaa
     1381 gccatatatt tagccgatca tggaatttat tgtgaagttg ttccaggaat cagctcctgt
     1441 atcgcagctg cggaacttgc ggggattcct gtaactcatc gaggaatctc caaaggattc
     1501 cgtgttgtga cagcacatga tcgaagaaat cagttatctg atcttaattt tgcatctatg
     1561 gcaaagtctg aagaaacctt agtgttctta atgggacttt cgaaactaga agagatcaca
     1621 acgaatctat taaaaaatgg aatggatgcc aatacacttn nnnnnnnnng ttgcagttgt
     1681 ttctagtgcg gcatctacaa agcagcaaac gtgtgaagca acattaaata cgattcatga
     1741 aaaagtaaag caagaaaaac tttcatcacc agcagtcatt gttgtaggag aggttgtgaa
     1801 acttcaaaaa cagattcaaa aaccagaaga tacaacctgt catttagtta ccaaaatagg
     1861 agatcagcca tcaaagttag cgcagatatt aaaagatcat ggatataaga tcaaggaact
     1921 tcagacagga gagatttctt atagatatga ccggatttca aaagaggaat tagcgaaagt
     1981 gacctatctg atttttacaa gcagatatgg agttcatggt tttatgaaac agatgaaatc
     2041 ttcgaatctg gatcttcgag atctcttcga taaaaaaatt gtcgttgtag gtaaaaagac
     2101 aaaagatgtc ttgatgcaat atgggattat agcagatctt atgccagaag aagcaggaga
     2161 gatcaatctt tccgaactta tgaaacagga agtagatgca aaggatgtgg tttggtactg
     2221 taaaggcata tctggtggag aaaaattaaa aaaagccctt acaccgatct gtcaggtaac
     2281 agaaaaggtc atgtatgaaa ataatagaaa gattttaagt gaaattccag aaatgaaaga
     2341 tatccgaagt gtttctttta cctgtgcatc atcagctcga agattttttg atcagatagg
     2401 agaagaaaag caacaatgga tagagaatat tccgtgaatc agtatt
//
LOCUS       DAOMUE010000302         2439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12338_length_2439_cov_2.132550, whole genome shotgun sequence.
ACCESSION   DAOMUE010000302 DAOMUE010000000
VERSION     DAOMUE010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12338_length_2439_cov_2.132550"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..492)
                     /locus_tag="PLC71_10255"
     CDS             complement(157..492)
                     /locus_tag="PLC71_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03060.1"
                     /translation="MHKNTDFHIEQTRLNDTYRKLNSGNDFSSYINEQENQSPTYWSF
                     LKLRIGFSILFLFFLIACFKTAPAKEFKKVQSAFSHINQTDSYSEHILNQFDLNFSSC
                     IDNLHKKKR"
     gene            655..1689
                     /gene="hrcA"
                     /locus_tag="PLC71_10260"
     CDS             655..1689
                     /gene="hrcA"
                     /locus_tag="PLC71_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRM03061.1"
                     /translation="MELNDRKKLILKAIIANYLETGEPVGSRTIAKHPELNLSSATIR
                     NEMADLEEMGYILQPHTSAGRIPSDTGYRFYVDQLMTERDSEHEEKQALIQRVDKMEV
                     LLKQVANALANSTNYATMVSSPHYQNVTLKFVQLSQVDHEHLLAVIVTQGNIVKNKMI
                     QVEEPLSNEDVLKLNVLLNTFLQGLTLQDINLALIQTMREQAGIRQDVLERILEEIAK
                     AIHDADHMEIYTGGATNILKYPELGNPDTATELLDTLVEKKALTKLVDSTLESDSDNH
                     TGIQVYIGEESPVKNMKDCSIVTATYELAEGGTGTVGIIGPKRMDYKKVVKTLKNLTE
                     ELDDIFKDKS"
     gene            1664..2284
                     /gene="grpE"
                     /locus_tag="PLC71_10265"
     CDS             1664..2284
                     /gene="grpE"
                     /locus_tag="PLC71_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRM03062.1"
                     /translation="MIFLKTNLKKGSDVMSKKNENINEEVKNAEETAEDIKDTETKEE
                     ASKETEETKEAAADDKIAELEASVADWKDKYQRLMAEFENARKRTAKEATQRYDMGAM
                     GVLEKLLPVIDNFERGLEAVSEEEKDSAFVKGIEQIYKQFVAVMEDVGVTPMDAQGKE
                     FDANLHNAVMHVEDDEFGENIVAEELQKGYMYKENVLRHSMVKVAN"
     gene            2334..>2439
                     /locus_tag="PLC71_10270"
     CDS             2334..>2439
                     /locus_tag="PLC71_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HRM03063.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGKPTVIANQEGART"
BASE COUNT          914 a          358 c          547 g          620 t
ORIGIN      
        1 aaagaataag aagaaaaccc tctatattaa agttcgccca tatcagacaa ttaataagaa
       61 gaacgtttac ggaagatggt cttatctgca gttatagtaa gaaaaatgaa aagaagtatt
      121 taaacaaaga agcgctagat tggtattttg ccaaaactag cgtttctttt tgtgaagatt
      181 atctatgcat gatgagaaat ttaaatcaaa ttgatttaga atatgctcac tgtaggaatc
      241 ggtctggttg atgtgggaaa aagcagattg aacttttttg aattctttgg caggagcagt
      301 cttaaagcat gcgattaaaa agaataaaaa caaaatagaa aagccaattc ttaattttaa
      361 aaaagaccaa tatgttggtg attggttttc ttgctcattt atataggaag aaaaatcatt
      421 accagaattt aattttcgat atgtatcatt aagtcgagtt tgttctatat gaaaatccgt
      481 atttttatgc atgaaaagta cctccttttg tacattctat tcagagaaga aaaaaactga
      541 acaaattttc aaaaaaattg aaagaaaagg ttgacattaa aatgcgaaag tgctacttta
      601 aatatagatg ttagcactcg atataaatga gtgctaataa caggaggcga agatatggag
      661 ttaaatgatc gtaagaagtt aatattgaaa gcgattatcg ccaattacct tgaaactggt
      721 gagccggtag gatcaagaac cattgccaaa catcctgagt tgaatttaag ctcagcaacg
      781 atccgtaatg agatggcaga tctggaagag atgggatata tcttacagcc tcatacatcc
      841 gcaggaagaa ttccttccga cacaggttat cgtttttatg tagatcagct gatgacggaa
      901 cgtgacagcg aacacgaaga gaagcaggca ttgattcaga gagttgataa gatggaagtc
      961 ttattaaaac aggttgccaa tgcattggca aacagcacga attatgcaac gatggtttct
     1021 tccccacatt atcagaatgt aacactgaaa tttgtgcagc tgtctcaggt ggatcacgaa
     1081 catctgcttg cagtgatcgt tacacaaggc aatatcgtaa agaacaagat gatccaggta
     1141 gaagaaccgc tttcgaatga agatgttctg aaattgaatg tattgttgaa tacattttta
     1201 caaggattaa cattacaaga tatcaacctt gcactgattc agacgatgag agagcaggca
     1261 gggatcagac aggatgtgtt agaacggatt cttgaagaga ttgccaaagc gatccatgat
     1321 gcagatcata tggaaatcta cacaggagga gcgaccaata tcttaaaata tccggagctt
     1381 gggaatccgg atacggcaac agaacttctt gatacattag ttgagaagaa ggcactgacg
     1441 aaactggttg attcaacttt ggaatcagat tctgataatc atacagggat ccaggtttat
     1501 atcggagaag aatctccggt taagaatatg aaggactgta gtatcgtaac tgcaacatat
     1561 gagcttgcag aaggaggtac aggtaccgtt ggtatcatcg gaccgaagag aatggattac
     1621 aagaaagttg ttaagacttt aaagaacctc actgaagaat tagatgatat ttttaaagac
     1681 aaatcttaag aaaggaagtg atgtgatgag taagaaaaac gaaaacatca acgaagaagt
     1741 aaagaacgca gaagagactg ctgaagatat aaaagataca gaaacaaaag aggaagcttc
     1801 caaagaaaca gaagagacaa aggaagcagc cgctgatgac aagattgcag aattagaagc
     1861 gtccgtagct gactggaaag acaaatatca gagactgatg gctgaatttg agaacgcaag
     1921 aaagcgtaca gcaaaggaag ctacacagcg ttatgatatg ggagcaatgg gagttttaga
     1981 aaaacttctt ccagttattg ataactttga gagaggctta gaagccgtat ccgaagaaga
     2041 gaaagactcc gcattcgtta aaggaatcga acagatctat aaacagtttg tcgctgtaat
     2101 ggaagatgtg ggagtaacac caatggatgc gcaaggaaaa gaattcgatg caaatcttca
     2161 taacgcagtg atgcatgtcg aagatgatga gttcggtgag aacatcgttg cagaagaatt
     2221 acagaaaggt tacatgtaca aagaaaatgt actgcgccat agtatggtaa aagtagctaa
     2281 ctaatattta tattatatat agaataagaa gaaatcttag gaggacatag atcatgggta
     2341 aaattattgg tattgactta ggaacaacaa acagttgtgt agcagttatg gaaggtggaa
     2401 aacctaccgt tatcgcaaat caggaaggtg caagaacaa
//
LOCUS       DAOMUE010000303         2436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12351_length_2436_cov_3.075598, whole genome shotgun sequence.
ACCESSION   DAOMUE010000303 DAOMUE010000000
VERSION     DAOMUE010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12351_length_2436_cov_3.075598"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..>1518
                     /locus_tag="PLC71_10275"
     CDS             22..>1518
                     /locus_tag="PLC71_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRM03064.1"
                     /translation="MVKPMMEINGFKVRPGFFDLNGASEFSCGVNFTVHTSNGTSCDL
                     LLFHPGEEEPYAIIPFPESYKIGDVYSMIVYDLKSEDFEYAYRVDGPYDEQKGLLFDK
                     TKVLLDPYAQAVAGQEVWGHKRTRTYHARVVRDSFDWGVQPQSTREMSDLIIYELHVR
                     GFTNHSSSGVKHPGTFAGLKEKIPYLKELGINAVELMPIFEFDEMINAREVDGKQLVE
                     YWGYNTVGFFSPNASYTAAEEVNNEGQELKELIRELHENGIEVILDVVFNHTAEGNEN
                     GPFFSFKGFDNNIYYLLTPEGNYYNFSGCGNSLNCNHPVVQQMILECLRHWTVHYRVD
                     GFRFDLASILGRDEDGMPMNNPPLLKSLAYDPLLRNVKLIAEAWDAGGLYQVGNFPAS
                     KRWAEWNGQYRDTMRGYLKGDFWEANSAAWRICGSGDLYGGYYSDGNSNYAGYNSCIN
                     FLTCHDGFTMYDLYSYNNKHNEANGWNNTDGANDNRSWNCGMEGDTKDP"
     assembly_gap    1519..1528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1529..2129
                     /locus_tag="PLC71_10280"
     CDS             <1529..2129
                     /locus_tag="PLC71_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HRM03065.1"
                     /translation="EVLKLRYRMIRNACAILMCSRGTPMFFSGDEFGNTKFGNNNSYC
                     QDNEISWIDWSLLEKNKDLFEFFKFMIDYRKKHPVIRKKLDNAVCGMEAMHAHDVNAE
                     RMEVPQNAKTLAVSFAGYDRKKGKDDLVYVAVNAYWEEVKITLPNLANHGAWYLSVDT
                     YGDEKGKYFYQEGEEIRIDREYVMKPRSIVVFTGREILR"
BASE COUNT          824 a          370 c          542 g          690 t
ORIGIN      
        1 tatattacag acgatgtcag aatggtaaag ccaatgatgg aaatcaatgg ttttaaagta
       61 aggccaggat tttttgatct aaacggagca tcagaatttt catgtggagt gaattttaca
      121 gtgcatacga gcaatggaac atcatgtgat ctgctattat ttcatccagg agaagaagaa
      181 ccatatgcaa tcattccatt tccagaatca tataagatcg gagatgtcta ttctatgatc
      241 gtttatgatc tgaaatccga agattttgaa tatgcttatc gtgtggatgg accatatgat
      301 gaacaaaaag gattactatt tgataaaaca aaagttttgt tagatccata tgctcaggcg
      361 gttgcaggac aggaagtctg gggacataaa agaaccagaa cgtatcatgc aagagtggta
      421 agagatagtt ttgactgggg tgtacagcca cagtcaacca gagagatgag tgatctgatc
      481 atttatgagt tacatgtcag aggatttaca aatcattcat cctcaggagt caaacatcca
      541 ggaacttttg caggattaaa agaaaagatc ccatatttaa aagaattagg gatcaatgcg
      601 gtagaattga tgccgatctt tgaatttgac gagatgatca acgcaagaga agtggatggg
      661 aaacaacttg ttgaatattg gggatataat acggttggat ttttttcacc aaatgccagt
      721 tatacagctg ctgaggaagt caataatgaa ggacaggaat taaaagaatt gatccgtgaa
      781 cttcatgaaa atggaatcga agttattctg gatgttgtat ttaatcacac tgcggaagga
      841 aatgaaaatg gaccattctt cagttttaag ggatttgaca acaatatcta ctatttatta
      901 actccagaag gtaattatta taattttagt ggatgtggga attctctaaa ttgcaaccat
      961 ccagtagtac agcagatgat ccttgaatgt ttaagacatt ggacggttca ttatcgtgta
     1021 gatggattcc gatttgatct tgcaagcatt cttggacgag atgaagatgg aatgccaatg
     1081 aataatccac cattactaaa aagtttagcc tatgatccac ttctaaggaa tgtaaaactg
     1141 atcgcagaag catgggatgc aggtggatta tatcaggtag gaaactttcc agccagcaaa
     1201 cgatgggcag aatggaacgg acagtaccgt gatacgatgc gaggctatct aaaaggtgat
     1261 ttctgggaag ctaacagtgc tgcatggaga atttgtggtt caggtgactt gtatggcgga
     1321 tattactctg atggaaacag taattacgct ggatacaatt cctgtatcaa cttcctcaca
     1381 tgtcacgatg gatttacaat gtatgatctc tattcttaca ataataaaca taatgaggca
     1441 aatggttgga acaataccga tggggcaaat gacaatcgaa gctggaattg tggtatggaa
     1501 ggagatacaa aagatccgnn nnnnnnnngg aagtactcaa attacgttac cgcatgatca
     1561 gaaatgcttg tgctatctta atgtgcagcc gtggaacgcc aatgttcttt tcaggcgatg
     1621 aatttggaaa tacgaagttt ggaaataata acagctactg tcaggataat gaaatctcat
     1681 ggattgattg gagcttatta gagaagaata aagatttatt tgaattcttt aaatttatga
     1741 tcgattacag aaaaaaacat ccagttatcc gcaagaaact ggacaatgca gtttgcggaa
     1801 tggaagcaat gcatgcacac gatgttaatg cagaacgtat ggaagtacca caaaatgcaa
     1861 aaacactggc agtaagcttt gcaggatatg accgcaaaaa aggaaaagac gatcttgtat
     1921 atgtagcagt caatgcttat tgggaagaag taaagatcac acttccaaac cttgcaaatc
     1981 atggagcatg gtacttaagt gtggatactt atggagatga gaaaggaaag tacttctatc
     2041 aagaaggaga agagattcgc attgatcgtg aatatgtgat gaaacctcgg tctattgtgg
     2101 tgtttactgg acgagaaatt ttaagataaa ttttaatttt aaatttgtag ttggccaact
     2161 gtagattttg agtatatgat aaaaaataaa taagcaaccg aaagcacagc ggtggaattt
     2221 tgctcacatt ttgaacaaat cgctgatccg cccaatttcc tacgagaact cgctctgaag
     2281 ttttgtttca gacaaaagtc tttgaagctc agacactcgt ccaattgggc tgcgaatttg
     2341 ttcaaaatat ggcaaaattc caatgctgcg ttttcggttg cttatttatt ttttatcata
     2401 taccagaagt ctaaagtgtg gacaattaca agattg
//
LOCUS       DAOMUE010000304         2436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12354_length_2436_cov_1.957581, whole genome shotgun sequence.
ACCESSION   DAOMUE010000304 DAOMUE010000000
VERSION     DAOMUE010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12354_length_2436_cov_1.957581"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PLC71_10285"
     CDS             <1..859
                     /locus_tag="PLC71_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRM03066.1"
                     /translation="MIKTGYRLLQVDTTNTASQIVFAGIRFALAGLFVLIFASIQEKK
                     VTLPDGKILKYAVPVCLAQTVGQYFFFYIGVAHTSGVKGGIITGLGNFIAILMSCLIV
                     RNEKLTGRKLTGCILGFAGVVVINLLGNSLDFGFTMMGEGFVLISQLAYATSTILINY
                     YSKKVSPVILSGTQFFIGGIVLFLIGNAMGGHLDHVTVGGITVVLYLALVSAVAYTLW
                     SVLLAYNDVSKVAIFGFVNPLCSVILSALVLGEVKQAFNIGSLIALALVCVGIYIVNA
                     KKNNKSNVE"
     gene            873..1556
                     /locus_tag="PLC71_10290"
     CDS             873..1556
                     /locus_tag="PLC71_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HRM03067.1"
                     /translation="MRYEILGETLPVVICYLESGEKMVTEGGGMSWMSPNMKMETTSN
                     GGIGKIFGRAFSGEKMFQNVYTAQGGEGMIAFASCFPGSIKAFEIAPGQEMILQKSAY
                     LAGENGVELSAFFNKKIGAGLFGGEGFIMQKASGHGIMFAEFDGHVVEYELEAGEQIV
                     VDTGHLAAMTASCTMEIKSVPGVKNMLFGGEGVFNTILTGPGKVWLQTMPISNVAGVL
                     SPYFPSSSK"
     gene            1596..1829
                     /locus_tag="PLC71_10295"
     CDS             1596..1829
                     /locus_tag="PLC71_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03068.1"
                     /translation="MAFFDYDRNGKKDWKDNAREYYLYNKLKEDDASKHSQGKKESSD
                     DGKSNVGCGSVLLCFLFMFCMMWLAMMVDSLCK"
     gene            1844..>2436
                     /locus_tag="PLC71_10300"
     CDS             1844..>2436
                     /locus_tag="PLC71_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HRM03069.1"
                     /translation="MTKTKAYIAIDLKSFYASVECKERNRDPLTTNLVVADQSRTEKT
                     ICLAVSPSLKSYGIPGRPRLFEVVQKIKEVNNTRRWKALNRTFTGSSDDSTELNANPA
                     LKVDYIVAPPRMEYYLEYSSKIYNIYLKYIAPEDIFPYSIDEVFIDATDYLNTYQMTA
                     RELAMTMIRDVLKTTGITATAGIGTNMYLCKIAMDIVA"
BASE COUNT          780 a          357 c          577 g          722 t
ORIGIN      
        1 aatgatcaag acaggatatc gactgttaca agtagatacg acaaacacag ccagtcagat
       61 cgtatttgca ggaataagat ttgcgttagc aggacttttc gtcttgattt ttgcttctat
      121 acaggaaaag aaagtgacac tgcccgatgg aaagattcta aaatatgcag ttcctgtctg
      181 ccttgcacag actgttggac agtatttttt cttttatatc ggcgtggcac atacatctgg
      241 agtgaaaggt ggaatcatta caggtttagg aaattttatc gcaatcctaa tgtcatgttt
      301 gatcgtacga aatgaaaaac taacgggaag aaaattaaca ggatgcatct taggatttgc
      361 aggagttgtc gtgatcaatc ttcttggaaa ttctttggat ttcggcttta cgatgatggg
      421 agaaggattc gttttgattt cacagttagc ctatgcaaca tccacgatat tgatcaatta
      481 ctattcaaaa aaagtatcac cagttatatt aagtggaact cagtttttta ttggtggaat
      541 tgtattattt ttaatcggaa acgcaatggg tggacattta gatcatgtaa cagttggtgg
      601 aattacggtc gtgttgtatc ttgcattggt atctgcagtt gcctatacac tttggtctgt
      661 attacttgca tataatgacg tgtcaaaggt agcaatcttt ggatttgtga atccattatg
      721 cagtgtaatc ttgtcggcat tggtattggg agaagtcaaa caggcattta atatcggaag
      781 tctgattgca ctcgcacttg tatgtgttgg aatttatatt gtaaatgcaa agaagaataa
      841 caaatcaaat gttgagtaag gaggaaaaca ttatgagata tgaaatttta ggtgaaacac
      901 ttccagtagt tatctgttat ctggaatctg gagaaaaaat ggttacagaa ggtggaggaa
      961 tgtcttggat gtctccaaat atgaaaatgg aaacaacttc gaatggggga ataggaaaga
     1021 tctttggaag agcgttttca ggagaaaaga tgttccagaa tgtttataca gcacaaggcg
     1081 gagaagggat gatcgccttt gcttcatgct tcccgggatc catcaaggca tttgaaattg
     1141 cacctggaca ggaaatgata ttacaaaaaa gtgcttatct tgccggtgag aatggagttg
     1201 agttatctgc attctttaat aaaaagatcg gagctggatt atttggcgga gaaggtttta
     1261 ttatgcagaa agcatctggc catggtatca tgtttgcaga atttgatggt catgttgtgg
     1321 aatatgaatt agaggctgga gagcagattg ttgtagacac tggacatttg gccgcgatga
     1381 cagcaagttg tacgatggag atcaaaagtg ttccgggtgt aaagaatatg ttgtttggtg
     1441 gagaaggagt gtttaacaca atacttacag gccctgggaa agtatggctg caaacaatgc
     1501 caatcagtaa tgtagctgga gtattaagcc catattttcc aagctctagt aaatagaaaa
     1561 aacatgaata gattaaggaa tggggagtga tcaatatggc cttttttgac tatgatcgaa
     1621 atgggaaaaa agattggaaa gacaatgcca gagaatatta tctttataac aaattaaaag
     1681 aagatgatgc atcgaagcat agccaaggga agaaggaatc atccgatgat gggaaatcaa
     1741 atgttgggtg tggttctgta ctattgtgtt ttttatttat gttttgtatg atgtggcttg
     1801 ctatgatggt tgattcatta tgcaaataga ggagcataga acgatgacaa agacaaaggc
     1861 ttatattgcg attgacctca aatcattcta tgcttctgtg gaatgtaagg agcgaaatcg
     1921 cgatccttta acgacaaatc ttgtggttgc agatcaaagc cggacggaga agacgatctg
     1981 ccttgcggta tctccatcgc tgaaaagcta tggaatacca ggcagaccac gtttgtttga
     2041 agtcgtgcaa aagattaaag aagtcaataa tacccgcagg tggaaagctt taaacagaac
     2101 atttacaggt tcgtcggatg acagcacaga attaaatgca aatccggcat tgaaggttga
     2161 ttatattgtt gcaccaccac gtatggaata ctatttggaa tatagttcca agatttataa
     2221 tatttatctg aagtatattg cgccagaaga tatttttccg tactcaattg atgaagtatt
     2281 tatagatgca acagattatc tgaatactta tcagatgact gcaagggaac ttgccatgac
     2341 tatgatacga gacgttttaa aaacaacagg aattacagca accgccggaa tcggaacgaa
     2401 tatgtatctg tgtaagattg cgatggatat cgtagc
//
LOCUS       DAOMUE010000305         2427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12415_length_2427_cov_1.695194, whole genome shotgun sequence.
ACCESSION   DAOMUE010000305 DAOMUE010000000
VERSION     DAOMUE010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12415_length_2427_cov_1.695194"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>666
                     /locus_tag="PLC71_10305"
     CDS             <1..>666
                     /locus_tag="PLC71_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Lon protease"
                     /protein_id="HRM03070.1"
                     /translation="SRFVSLVRWLGENHIHVRLSGENTVEGYCVYKIREIALGGGTKL
                     SAEDGFLQFMIERLFQSDAPDERITDENLDEMGDDMKLTSIQSITDFMTCAGSTLPDN
                     IRLWARRNLAVAKSHEVSQEERRHAQRALSIMMNIQWKSNYFKAIDPEEARRILDEEL
                     YGMERVKQRIIETIIQINRTHTLPAYGLLLVGPAGTGKSQIAYAVARILKLPWTTLDM
                     SSI"
     assembly_gap    667..676
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <677..1250
                     /locus_tag="PLC71_10310"
     CDS             <677..1250
                     /locus_tag="PLC71_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM03071.1"
                     /translation="EQLTGSSRIYANAKPGIIMDAFSMAGESNLVFIINELDKANSGK
                     GNGNPADVLLTLLDNLGFTDNYMECMIPTVGVYPIATANEKDQISAPLMSRFAVIDIP
                     DYTPEEKKAIFQRFALPKVLKRMGLKDDECIMTDEALDAVIEMYSETTGIRDLEQAAE
                     HIAANALYQIEVHHMNSVTFDGAMVRNLLL"
     gene            1335..1490
                     /locus_tag="PLC71_10315"
     CDS             1335..1490
                     /locus_tag="PLC71_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03072.1"
                     /translation="MDMEKIYLTMKRTGAGNIVLGILSICIGLASGIMLIITGANLLK
                     KKKHLSF"
     gene            complement(1723..>2427)
                     /locus_tag="PLC71_10320"
     CDS             complement(1723..>2427)
                     /locus_tag="PLC71_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRM03073.1"
                     /translation="ILLTPFMSCTAKLPIYSFFVNAFFPKRGGLIMAGLYLAGIMIGI
                     LIAFLYKGTLFKGEPVPFVMELPNYRLPGVKNVAQLLWEKAKDFLQKAFSVILVATIV
                     VWFLQRFDLQLNMVEDSAHSILAMVAGVLVPVFSPLGLGDWRIVTSLISGFMAKESVV
                     STLGVLFNGNITAAITPLAASSLLVFSLLYSPCVAAIASVKRELGTRWAVGLALWQCM
                     IAWIAALIVRFIGLVI"
BASE COUNT          820 a          442 c          454 g          701 t
ORIGIN      
        1 attcccgttt tgtaagcctg gtcagatggc ttggagagaa tcatattcat gtccgcttat
       61 ctggggagaa tacagtagaa ggttattgtg tatacaagat cagagaaatt gctcttggcg
      121 gtggcaccaa attatctgca gaagatggtt tccttcagtt tatgatcgaa cgtttattcc
      181 aaagtgatgc accagatgaa cggatcactg atgaaaatct tgatgagatg ggtgatgata
      241 tgaaacttac cagcatccag agtatcacag attttatgac atgtgccgga agtacgctgc
      301 ctgataatat tcgtctatgg gcaagaagaa atcttgctgt tgcaaaatct catgaagttt
      361 cacaagaaga gagacgtcat gcccagcgtg cattatccat catgatgaac atccaatgga
      421 agagtaatta ttttaaagcg atcgatcctg aagaagcaag acggattctt gatgaagaac
      481 tctatggtat ggaacgtgtg aaacaaagaa tcattgaaac gatcattcag atcaatcgta
      541 cacacacgct gcctgcttat ggattattat tagttggacc tgctggaaca ggaaaatctc
      601 agattgctta tgcggttgca agaatcttaa aacttccatg gacaactctt gatatgagtt
      661 ccattannnn nnnnnnagag cagttaacgg gaagttcccg tatttatgca aatgcaaaac
      721 cagggatcat catggatgca ttttccatgg caggagaatc aaatcttgta tttatcatta
      781 acgagcttga taaagccaat tcaggaaagg gaaatggaaa tcctgcagat gttttactaa
      841 cattgcttga taatctcgga tttaccgata attatatgga atgtatgatc ccaacagttg
      901 gagtatatcc aattgccact gcaaatgaaa aagaccagat cagtgcacca ttgatgtcac
      961 gttttgcagt cattgacatc cctgattaca caccagaaga gaagaaagcg atcttccaaa
     1021 gatttgcact ccctaaggta ttaaaacgta tgggattaaa agatgatgaa tgtattatga
     1081 cagatgaggc cttagatgct gtcattgaga tgtattctga aaccacagga atcagagatc
     1141 tggaacaagc cgcagaacat attgcagcca atgcattata tcagattgaa gtccaccata
     1201 tgaatagtgt aacatttgat ggtgcaatgg taagaaatct gcttttataa gttgttattt
     1261 gacgaagtat gaattttaat gatatagtaa atcatagaca ttacaaatta taattctcaa
     1321 acggaggtca catcatggac atggagaaaa tctacttaac aatgaaacgt accggtgctg
     1381 gaaacatcgt gcttggaatc ttatccattt gtattggact tgcatctgga atcatgctga
     1441 tcatcacagg tgcgaatctg ttaaagaaaa agaagcattt aagtttctaa ataacatctg
     1501 aatgataaag gaacatggaa ttggaataag attttcaatg ttcctttatt tttttacatt
     1561 tgacaataat gatataaaat atggaaagaa cagaccttga aagtgccata tgcttaagaa
     1621 tcaatctata cgcaaaatac cgatttaaat tatagattgc tgataaaaga cagagaagcc
     1681 agtattttac tggcctctct cgatattttt attttatgta attcaaatta ctaatccaat
     1741 gaatcttacg attaatgctg caatccatgc aatcatacat tgccataatg ccagaccaac
     1801 cgcccatctt gttcctagtt cacgttttac agatgcgatg gccgcaacgc atggagaata
     1861 aagcaaacta aatactaata aagaacttgc ggctaatggt gtgatcgccg ctgtgatgtt
     1921 tccattgaat aaaactccta atgttgaaac tacactttct tttgccataa atccactgat
     1981 caatgatgtc acgattctcc aatctcccaa tcctaatggt gaaaaaactg gtactaacac
     2041 accagctacc attgctaaaa tgctatgtgc agaatcttct accatattta actgcagatc
     2101 aaatctctgt aaaaaccata caacgatcgt ggcgaccaaa atcacagaaa atgctttttg
     2161 taagaaatct tttgctttct cccataataa ttgtgctaca ttcttcactc ctggaagtct
     2221 gtaatttggt aactccataa caaatggcac tggttctcct ttaaataacg ttcccttata
     2281 taaaaatgca atcaaaatac ctatcatgat tcctgctaaa tataatcctg ccataataag
     2341 accaccacgt tttggaaaaa aggcattcac gaaaaaagaa taaattggca gcttggctgt
     2401 acagctcata aatggtgtta aaagaat
//
LOCUS       DAOMUE010000306         2416 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12483_length_2416_cov_2.335451, whole genome shotgun sequence.
ACCESSION   DAOMUE010000306 DAOMUE010000000
VERSION     DAOMUE010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12483_length_2416_cov_2.335451"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /gene="thrS"
                     /locus_tag="PLC71_10325"
     CDS             <1..1193
                     /gene="thrS"
                     /locus_tag="PLC71_10325"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRM03074.1"
                     /translation="GKELGLFMMREEGPGFPFFLPKGMVLKNTLLDYWREIHQKAGYV
                     EISTPIMLSCHLWETSGHWDHYKENMYTTVIDDQDFAIKPMNCPGGVLVYDSEPRSYR
                     DLPLRMGELGLVHRHEKSGQLHGLMRVRCFTQDDAHIFMTPDQIKDEIKGVAGLIDQV
                     YNLFGFKYHVELSTRPDDSMGSDEDWELATDSLRAALDDLGLDYVVNEGDGAFYGPKI
                     DFHLEDSIGRTWQCGTIQLDFQLPLRFDLHYTGADGEKHRPIMIHRVAFGSIERFIGI
                     LIEHFAGAFPTWLAPVQVEVIPISDKHLDYANKVLDTLKAAGIRADIDTRAEKMGYKI
                     REAQLQKIPYMLVVGGKEEENEAVSVRSRFKGDEGQMKLDDFLDAIKEEIANRENRKV
                     EKED"
     gene            complement(1246..1653)
                     /locus_tag="PLC71_10330"
     CDS             complement(1246..1653)
                     /locus_tag="PLC71_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRM03075.1"
                     /translation="MKILIDTNVILDVLCDRTDFVESSSKIWKLCEVEKIDGYISALS
                     IPNIVYILRKELTPEKTQQIIEQLFMIFHIADLKSSDIRKAANMKTSDYEDAIQMVCA
                     QRMKVDFIVTRNIKDFIESKVPAMKPDELLERI"
     gene            complement(1637..1873)
                     /locus_tag="PLC71_10335"
     CDS             complement(1637..1873)
                     /locus_tag="PLC71_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HRM03076.1"
                     /translation="MITTTATDIQNNFGKYLKAVQEGNEIIILKKGKEVARLISKDTG
                     ISFLTDSLTGILKKDYNEKDIRAERIAEKYENID"
     gene            2078..2386
                     /locus_tag="PLC71_10340"
     CDS             2078..2386
                     /locus_tag="PLC71_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HRM03077.1"
                     /translation="MEEKKECCCHHKTKERSDKEYKDLINRLSRIEGQVRGIKKMVEN
                     DTYCTDILVQVSAVNAALNSFNKVLLANHIRTCVAEDIREGKDETIDELVKVLQKLMK
                     "
BASE COUNT          816 a          385 c          448 g          767 t
ORIGIN      
        1 ttggaaaaga acttggtctg tttatgatga gagaagaagg acctggattc ccattcttcc
       61 ttccaaaagg tatggttctt aaaaacacat tattagacta ctggcgtgaa attcatcaga
      121 aagcaggata cgttgaaatc tcaacaccta tcatgttaag ttgtcattta tgggaaacat
      181 caggacattg ggatcattac aaagaaaata tgtatacaac agttattgat gatcaggatt
      241 ttgcaatcaa acctatgaac tgcccaggtg gagttcttgt ttatgattct gaaccacgtt
      301 catatagaga tcttccatta agaatgggag aattaggttt agtacatcgt catgaaaaat
      361 ctggtcagtt acatggatta atgcgtgttc gttgttttac acaggatgat gcacatatct
      421 ttatgacacc agatcagatc aaagatgaga tcaaaggtgt tgcaggattg atcgatcagg
      481 tttacaattt atttggattt aagtatcatg tagaactttc tactcgtcca gatgacagca
      541 tgggaagtga cgaagattgg gaacttgcaa cagattctct tcgtgcagct ttagatgatc
      601 ttggacttga ttatgtggta aatgaaggag acggagcatt ctacggacct aagattgatt
      661 tccacttaga ggattctatc ggaagaacat ggcagtgtgg aacgatccag cttgacttcc
      721 agctcccatt acgttttgac cttcactaca caggagcaga tggagagaaa catcgtccaa
      781 tcatgatcca ccgtgttgca tttggatcta tcgagcgttt tatcggtatc ttgatcgagc
      841 attttgcagg tgctttccct acatggttag ctccagtaca ggttgaagtg attcctatct
      901 ctgataagca tttagattat gctaataaag tacttgatac attaaaggca gcaggaatcc
      961 gtgcagacat cgatacaaga gctgagaaaa tgggatacaa gatccgtgaa gcgcagcttc
     1021 agaagattcc atatatgtta gttgttggtg gaaaagaaga agaaaacgaa gctgtatcag
     1081 taagaagccg attcaaaggt gatgaaggac agatgaagtt agatgacttc ttagatgcaa
     1141 tcaaagaaga gattgctaat agagaaaacc gtaaggttga aaaagaagat taattgtcaa
     1201 atgaaaagga ggcaccacat ttgtggaatg cctccttcct ttatatcata tccgttctaa
     1261 taattcatca ggtttcatag caggtacttt actttcgata aaatccttga tattacgagt
     1321 tacaataaaa tctactttca ttcgctgtgc gcataccatt tgaatcgcat cttcataatc
     1381 agaagttttc atattcgccg cttttcttat atcagaagac tttagatcag caatatgaaa
     1441 aatcataaac agctgttcaa taatttgctg agttttttct ggtgttaatt cttttctcaa
     1501 aatataaaca atatttggaa tagataatgc tgaaatatat ccatcaattt tttctacttc
     1561 acataacttc cagatttttg aagagctttc tacaaaatca gttctgtcac ataatacatc
     1621 aaggattaca tttgtatcaa tcaatatttt catatttttc tgcaatcctt tctgcgcgga
     1681 tatccttttc gttataatct ttttttagaa ttccagttaa agaatcagtc aagaaagaaa
     1741 tacctgtgtc ttttgaaatt aatctggcga cttcttttcc tttctttaaa ataataattt
     1801 cattaccttc ttggacagct tttaaatatt taccaaagtt attttgaata tcagttgctg
     1861 ttgttgtaat catagtatca cctcctattt taatagttag tacctaaaat tattatatgc
     1921 tattttaggg attttatcaa tgatattaga aaaattgatt cattttataa gttatataaa
     1981 acaagattgt ctatataact gcaacatttt ctcaaataaa catcttgaaa tataccctaa
     2041 cagggtatat aatatcaaac gtaaaggagg aaatcacatg gaagaaaaaa aagaatgttg
     2101 ttgtcatcac aagacaaaag aacgttcaga caaagaatat aaagatctta ttaatcgtct
     2161 aagccgtatt gaaggacaag ttcgtggaat aaaaaagatg gtagagaatg atacttattg
     2221 taccgatatt ttagttcagg tatctgctgt caatgcagct ttaaatagtt tcaataaagt
     2281 ccttcttgcc aatcatatca gaacttgtgt agcagaagat atccgagaag gaaaggatga
     2341 gacgattgat gaattagtaa aagttctaca aaaactaatg aaataacaat ttgacaagca
     2401 aaatgaaagg aaggga
//
LOCUS       DAOMUE010000307         2410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12518_length_2410_cov_2.716348, whole genome shotgun sequence.
ACCESSION   DAOMUE010000307 DAOMUE010000000
VERSION     DAOMUE010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12518_length_2410_cov_2.716348"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1577)
                     /locus_tag="PLC71_10345"
     CDS             complement(162..1577)
                     /locus_tag="PLC71_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRM03078.1"
                     /translation="MNILNGFNKVKQQTEDTSEGNMKHLKLGKIQWIALLVVSAMFVN
                     LCSHMVLAAARDGNDPAQGTTAEATTASEATTEATTASEATTETTTEQQEETQSMGEY
                     KAFWFSFYDYDSYRAKYKKRTAANFRTYFTGVVKKGKSLGMNRVIVQVRPFGDAIYKS
                     KYFPWSKYISGKQGRNPGFDPLKIMVEVAHDNDMKIEAWVNPYRVTTGSTNYKKLAKN
                     NQARKWHAKKSTRRNVLSYGGSLYYNPSKKAVRTLITNGVKEIVQNYDVDGIHMDDYF
                     YPSFTKRNVKKAFDAKEYNKSSYKKKKKSIYTYRRAQVNTLVKQMKKAVKSVDPNVTY
                     GISPAGNIDNLTSKYSYYVDIYKWLNSTEYVDYICPQVYWGFKHPTAKFNKVTDRWIK
                     AAKSKKVKLYIGIAVYRAGHNVGQNRAERKEWKRDTKVLKKQVQYARKKHVDGFAFFD
                     YQDLKSKTSAKAVNQLKTVLK"
     gene            complement(1555..2328)
                     /locus_tag="PLC71_10350"
     CDS             complement(1555..2328)
                     /locus_tag="PLC71_10350"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM03079.1"
                     /translation="MEKKYFFFDIDGTLTDRSTGKIVPSAREALDRLEANGHFVAIAT
                     GRAHYKARKFTEANGFKNMVCCGGNGCVINNELVENIPLDKEGCLQIIHQSEELGYGW
                     CVALDDTKDVYMTDDKFLRQVGKRKEPTRYIIDNMLDIDSVDAIYKIYVAIPKEEEEK
                     LTKKELIGHLRFEPEYLMFQPDNKRGGIVKMMEYLGADIKNVVVFGDDYNDMDMFCKD
                     WFSIAMGNGCQDLKDMASYVTDTNVNDGIKKACEHFEWI"
BASE COUNT          639 a          522 c          335 g          914 t
ORIGIN      
        1 ggcatcctcc ttgcatcata ttatatgcca tataatatta tatttttcat attatgtcaa
       61 ctcgctgtag atttcttata tagcgtaatt tacatgaaaa gaaatctgct gtaactaaaa
      121 agagaccatc acatatctct atgcaatgat ctctgtaata tttattttaa aactgttttg
      181 agctgattaa ccgctttggc acttgtcttg ctctttagat cctgatagtc aaagaatgca
      241 aatccatcca catgtttctt tcttgcatac tgtacctgtt tcttcaagac ttttgtatca
      301 cgcttccatt cttttcgctc tgcacgattc tgaccaacat tatgtcctgc acgatatact
      361 gcaattccaa tataaagttt aactttcttg ctctttgctg ccttgatcca acgatctgtg
      421 actttattaa attttgcagt tggatgttta aatccccagt atacttgtgg acagatataa
      481 tcaacatact ctgtagaatt tagccattta tagatatcta cataatatga atatttactt
      541 gtcagattgt caatatttcc tgctggactg ataccatatg ttacatttgg atctacactt
      601 tttactgctt ttttcatctg ttttacaaga gtatttacct gtgcacgacg gtaagtatag
      661 atacttttct ttttcttctt ataagaagat ttgttgtatt cttttgcatc aaatgccttt
      721 ttgacattac gttttgtaaa agatggatag aaataatcat ccatatgaat tccatcgaca
      781 tcataatttt gtacgatttc ttttactccg tttgtgatca gtgttctgac cgctttctta
      841 gatgggttgt aatacaaact tccaccataa gataatacat ttcttcttgt agacttcttg
      901 gcatgccatt ttcttgcctg attattcttt gcaagtttct tataattcgt agaacctgtt
      961 gttacacgat atggatttac ccatgcctcg atcttcatgt cattgtcatg tgcaacttct
     1021 accatgatct ttaatgggtc aaatcctgga tttcttccct gttttccaga aatatatttg
     1081 gaccaaggga aatactttga cttataaatg gcatctccaa atggtcttac ttgcacgatc
     1141 acgcgattca ttccaagact ctttcctttt ttgacaacac ctgtaaagta ggtacggaaa
     1201 tttgctgccg ttctcttttt atattttgca cgataagagt cataatcgta gaaagaaaac
     1261 cagaatgctt tatattctcc catagactgt gtctcttcct gctgttccgt tgtagtttct
     1321 gttgtcgctt ctgaagctgt tgttgcttct gttgtcgctt ctgaagctgt tgttgcttct
     1381 gccgttgttc cctgtgccgg atcatttcca tctcttgctg ctgccaatac catatggctg
     1441 cataaattca caaacattgc actgacaacc aataatgcga tccattgtat ctttcctaat
     1501 tttaaatgtt tcatatttcc ctccgatgta tcttctgtct gctgttttac tttattaaat
     1561 ccattcaaaa tgttcacatg ctttcttaat accgtcattg acatttgtat cggtcacata
     1621 acttgccata tcttttaaat cctggcatcc atttcccata gcaatggaaa accagtcttt
     1681 acagaacata tccatatcat tgtaatcgtc cccaaataca accacatttt taatatctgc
     1741 gccaagatat tccatcatct tcacaatacc accacgttta ttatctggct ggaacattaa
     1801 atattctggt tcaaatctca aatgcccgat caactctttc tttgtaagtt tctcttcttc
     1861 ctcttttgga atagctacat aaatcttgta aatcgcgtcg acactgtcaa tatctaacat
     1921 attatcaatg atatatctgg ttggttcttt tctctttcca acctgtctta gaaatttatc
     1981 gtctgtcata tagacatctt tcgtatcatc aagagctaca caccatccat accctaattc
     2041 ttctgactga tgaatgatct gtaaacaccc ttctttatcc aaaggaatat tttccactaa
     2101 ttcattgttg atcacacagc cattgcctcc acaacagacc atatttttaa acccattggc
     2161 ttctgtgaat ttacgtgctt tgtaatgtgc tcttccagtt gcgattgcca caaaatgtcc
     2221 atttgcttct aaacgatcca acgcctctct ggcacttggc acgatcttcc ctgtcgatct
     2281 atctgtcaat gttccgtcaa tatcaaaaaa gaaatatttc ttttccattg ttatctactc
     2341 cttataaact cttatcatag cattttattt attgcaatga atttaactgt tcataaaatg
     2401 ttggataaga
//
LOCUS       DAOMUE010000308         2406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12535_length_2406_cov_2.211399, whole genome shotgun sequence.
ACCESSION   DAOMUE010000308 DAOMUE010000000
VERSION     DAOMUE010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12535_length_2406_cov_2.211399"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLC71_10355"
     CDS             complement(<1..254)
                     /locus_tag="PLC71_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRM03080.1"
                     /translation="MLRAFLKKNMKLPELFEEKMRRLLGEDFSEYEKHLDDPAYYGIR
                     VNTLKISVEDFLKICPFHVTKVPWTDNGFYVDREEKPSKHP"
     gene            complement(235..1038)
                     /locus_tag="PLC71_10360"
     CDS             complement(235..1038)
                     /locus_tag="PLC71_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HRM03081.1"
                     /translation="MELQLWREMLTPYELAVDELVLKFRHIIREYRNVGRYSPIEQVD
                     GRVKSISSILDKMQRKKINVNDVEKELEDIAGIRLICQFVEDIDRVLEIIESRSDMEI
                     KSHKDYISHSKDSGYRSYHVIIYYEVNTIYGKKRIQAEIQIRTLAMNFWATVEHSLQY
                     KYKGDIPQHVTDKLLVASDAIDVLDHEMSTVRDEIMDAQDSFRKKSNLVSEILHTMQN
                     LYKVANRHEVLKIQDEFYKIYETGDMERLEHFNKQLDIIAEGYRAQSIS"
     gene            complement(1238..2194)
                     /locus_tag="PLC71_10365"
     CDS             complement(1238..2194)
                     /locus_tag="PLC71_10365"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRM03082.1"
                     /translation="MSVKILGSFLPEKSVSNDDLSKVMDTSDEWISSRTGIRSRHISI
                     EDTTSTMAVKAAEKALEDAGISAEELDHIFVATLSGDYATPSTACQVQKGIGAVNAVC
                     MDINAACSGFVFGLNTAVAYARAGMGKKMMIIGVETLSKILDWSDRSTCVLFGDGAGC
                     AIVEADEEREIFIDAGSDGARGDVLTCEERHLNNLLVKDDSPMQQVAMDGQEVFKFAA
                     RMVPKSINKVLDEAGVSKEEIKYFVLHQANKRIIEAAARRLKQPIEKFPMNIDRCANT
                     SSATIPILLDEINQKGMLNRGDKIVLSGFGGGLTWGSVYLTW"
     gene            complement(2317..>2406)
                     /locus_tag="PLC71_10370"
     CDS             complement(2317..>2406)
                     /locus_tag="PLC71_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004069008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03083.1"
                     /translation="MTDESQQKLQDTMEKIINESNGGLVCIII"
BASE COUNT          655 a          568 c          327 g          856 t
ORIGIN      
        1 ggatgttttg atggtttttc ttccctgtct acataaaatc cattatctgt ccaaggcact
       61 ttcgtcacat gaaacggaca gatcttcaaa aagtcttcca ctgagatctt tagtgtattg
      121 actctgatcc cataatatgc aggatcatct aaatgcttct catattccga aaaatcctcg
      181 cccaatagac gtctcatctt ctcttcaaat aattctggta atttcatatt cttcttaaga
      241 aatgctctga gcacgatatc cttctgcaat aatatccaac tgtttattaa aatgctctaa
      301 gcgttccata tctcctgtct catatatttt ataaaattca tcctgaatct ttaatacttc
      361 atgacgattc gcaactttat ataaattctg catcgtatga aggatctcag atacaagatt
      421 tgacttctta cggaatgaat cctgtgcatc catgatctca tcacgaactg ttgacatctc
      481 atgatctaag acatcgatcg catctgatgc aactaataat ttatctgtca catgctgtgg
      541 aatatcccct ttatacttat actgtaaaga atgttccacg gttgcccaga aattcatcgc
      601 aagtgtacgg atctgtatct ctgcctgtat ccgcttcttc ccataaatcg tattcacttc
      661 ataatagatg atcacatgat agcttcgata tccgctgtct ttggaatgtg aaatatagtc
      721 tttgtggctc ttgatctcca tatcagaacg actctctatg atctctaata cacgatcaat
      781 gtcttccaca aactgacaga tcaaacgtat tcccgcaata tcttccagtt ctttttctac
      841 atcattgaca ttgatcttct ttctctgcat cttatccaaa atacttgaga tagattttac
      901 acgtccatcg acttgctcga tcggtgaata tcttccgaca tttctgtatt ctcttataat
      961 atgtcgaaac tttaacacaa gttcatcaac tgccagctca tatggggtga gcatttctct
     1021 ccataactgt aattccatga attccctcct gtacttatcc actcattata tcatatttta
     1081 acttcaaaaa gaatcttgta ttacaggatt ctttcttctt acttcatatt ttttatttca
     1141 ttgaaatttc ctatgcaata aaaaaatagt caactcctat catcttatat gaaagctgac
     1201 tatttttatc ttcatttgta tcgtatttgt ttgcttctta ccatgtaaga taaacagaac
     1261 cccaagtaag tcctccgcca aaaccactta aaacaatctt atctcctcgg tttagcattc
     1321 ctttttgatt gatctcatct aaaagaatcg gaatcgttgc tgatgaagta ttcgcacaac
     1381 ggtctatatt catcgggaac ttttcaattg gctgcttcaa tcttctcgct gctgcttcaa
     1441 tgatcctttt gttagcctga tgtaatacaa aatatttgat ttcttctttg gaaacgcctg
     1501 cttcatcaag gactttgtta atactctttg gcaccatacg tgctgcaaat ttgaatactt
     1561 cctgcccatc catggcaacc tgttgcattg gagaatcatc ctttactaag agattgttta
     1621 aatgtctttc ttcacatgtt aaaacatctc ctcgtgctcc atcagatcct gcatcgataa
     1681 agatttctct ttcttcatcc gcttccacga tcgcgcatcc tgcaccatct ccaaataata
     1741 cacaagtact gcggtcactc cagtcaagaa tcttagaaag tgtctcaacc ccaatgatca
     1801 tcatcttctt acccattcct gctctcgcat atgcaactgc tgtatttaat ccaaaaacga
     1861 atcctgaaca tgctgcatta atatccatac agactgcatt gacagctccg attcctttct
     1921 gtacctgaca agccgtactt ggtgttgcat aatctcctga taatgttgca acgaaaatat
     1981 gatctaattc ttccgcactg attcctgcat cttctaatgc tttctctgca gctttcactg
     2041 ccatcgttga tgttgtatct tcaatcgaaa tgtgcctgct gcgaatccct gtcctggatg
     2101 aaatccattc atcacttgta tccatcactt tggaaaggtc atcgttgctc actgattttt
     2161 ctggaagaaa acttcctaat atttttacag acataactac tccacttatc ttaatcgctt
     2221 ctttttttct cttcttgatc tgtactcata aatgacagat caaaaacact ttgtctatca
     2281 aagtatattc tttttccatc cgtttgtcaa ttgttcttag ataatgatac agacaagacc
     2341 gccattactt tcatttatga tcttttccat tgtatcctga agcttttgct gactctcatc
     2401 tgtcat
//
LOCUS       DAOMUE010000309         2347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12960_length_2347_cov_2.461169, whole genome shotgun sequence.
ACCESSION   DAOMUE010000309 DAOMUE010000000
VERSION     DAOMUE010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12960_length_2347_cov_2.461169"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLC71_10375"
     CDS             complement(<1..575)
                     /locus_tag="PLC71_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRM03084.1"
                     /translation="MIQIYKNYEGHKELEELDHIELESWVMITDPTMEELMNISRDYK
                     IDMDDLKAALDTEERSRLENEDDYTMILVNIPVVEEEEGKKWYETIPLGIFITNKIVF
                     TVCLEQTNILKQFVDGLVSNFYTFMKTRFVYQILYNNAALFLYYLRVIDQRANQVEDL
                     LHETYENKDLIQLYDLEKCLVYFTTALKSNEV"
     gene            complement(703..1668)
                     /locus_tag="PLC71_10380"
     CDS             complement(703..1668)
                     /locus_tag="PLC71_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRM03085.1"
                     /translation="MSTWGILGCGTIANEMAATFQKMGHEIYGVANRTPEKAEKFADK
                     YGVKHVFKTYEEMLADENIDIIYVATPHSMHYENMKQAVNAGKHILCEKAITVNAAQL
                     EEVLELAEEKGVVVREAMTISHMPLYKKLKERIKEGAIGNTKMVQVNFGSNKGYDSTQ
                     RFFALEAAGGALLDIGVYATAFARTFLSEMPNTILTTVEYLETGADEQSGIIMKNTKG
                     EMVVMCLTLRAKQPKRGVVTGDKGYIEVYEYPRACKATITNTATGEVETVEAGKTEDA
                     LKYEVEAMEAAIEGNLEDDCQIITKDTMRILTSIKTQWGMKYPFE"
     gene            complement(1687..2304)
                     /gene="rpiA"
                     /locus_tag="PLC71_10385"
     CDS             complement(1687..2304)
                     /gene="rpiA"
                     /locus_tag="PLC71_10385"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HRM03086.1"
                     /translation="MKRLCAKAALDLVPDHGIIGLGGGETISYLAEDIKEARKDVKVV
                     TPSDHTAQVCKKLGLEIVDTKEVKEIEIAFDGCDQVDSDLDAYKSGGGIHTLEKIIAK
                     MAKEYVLLVDETKVTDKLNNKVPVVLDIIPEAVNYVTSQVEAMGATVKKRDEHLLEVY
                     YDTLPELEEIDKNLKMITGVIETSLFYQVATKALVAGEEGIRVIK"
BASE COUNT          604 a          558 c          300 g          885 t
ORIGIN      
        1 acttcatttg attttaatgc tgttgtaaaa taaactaggc atttctctaa atcatacaat
       61 tggatcaagt ctttgttctc atatgtctca tgcaacagat cttccacttg atttgctctc
      121 tggtcgatca cacgcagata atacaggaac aacgctgcat tattgtaaag aatctgatat
      181 acaaatcttg tcttcataaa tgtataaaaa tttgatacca gtccatcaac aaattgtttt
      241 aagatattcg tctgctccaa acaaacagta aagacgatct tattagtaat aaaaatccca
      301 agcggtatcg tctcatacca tttctttcct tcctcttcct ctacaaccgg aatattcaca
      361 aggatcattg tataatcatc ttcattctcc agtcgggaac gttcttccgt atccagtgct
      421 gccttaagat catccatatc aatcttataa tctctcgaaa tattcatgag ttcctccatc
      481 gttggatctg tgatcatcac ccatgattcc aattcgatat gatctaattc ttctaactct
      541 ttatgtcctt cataattctt ataaatctgg atcatcttac cgctccttcc tgcttcattc
      601 ttattaagta taactgatag caaaagcttc gtccagtaaa aaaagaaatg tgtgtttggc
      661 acatttcttt ttgataaatt cttatatgat catctcagat ccttattcaa atgggtattt
      721 cattccccac tgtgtcttaa tacttgttaa aattcgcatc gtatcttttg taatgatctg
      781 gcaatcatcc tccaagtttc cttcgatcgc tgcttccatt gcttctacct catattttaa
      841 agcatcttct gtctttcctg cttcaactgt ctcaacttct ccagttgccg tattagtgat
      901 cgttgctttg catgctcttg gatattcata tacttcaata tatcccttat cacctgtcac
      961 aactcctcgt tttggctgtt ttgcacgaag tgtcagacac ataacaacca tctccccttt
     1021 tgtattcttc ataatgatcc cagattgttc atctgcacct gtctctaaat attctacagt
     1081 cgtaagaatc gtatttggca tctcacttaa aaatgttctc gcaaatgctg ttgcatatac
     1141 tccaatatcc aataaagctc cgccagctgc ttctaatgca aaaaatctct gtgtagaatc
     1201 ataacctttg ttacttccaa agttcacctg taccatcttt gtattaccga tcgcaccttc
     1261 tttgattcgt tcttttaatt tcttatataa aggcatatgt gaaattgtca ttgcttctct
     1321 gactaccaca cctttttctt cagcaagttc cagcacttct tctaactgtg ctgcattcac
     1381 tgtgatcgct ttttcacata agatatgctt tcctgcattg actgcctgct tcatattttc
     1441 ataatgcata ctatgtggtg ttgcaacata aatgatatcg atattttcat ctgccagcat
     1501 ctcttcgtat gttttaaaaa catgttttac tccatatttg tctgcaaact tttctgcttt
     1561 ctctggtgta cgattggcta ctccgtaaat ttcatgtccc atcttctgaa atgttgctgc
     1621 catctcattc gcaattgttc cacaacctaa aattccccat gtactcatct tatctttcct
     1681 tctttcctac ttgatcactc tgattccttc ttcacctgca accaatgctt ttgttgcaac
     1741 ctgataaaat aatgatgtct cgatcacccc tgtgatcatc tttaagtttt tgtcaatctc
     1801 ttctagttct ggtaaagtgt cataataaac ttccagtaaa tgttcatcac gtttcttcac
     1861 tgttgcaccc attgcttcca cctgacttgt cacatagttc actgcttctg gaataatatc
     1921 taatacaact ggtactttat tatttaactt atctgttact ttcgtttcat cgaccagtaa
     1981 tacatattct tttgccatct ttgcaataat cttctctaat gtgtgaattc cgcctccact
     2041 tttatatgca tcaagatcag aatcaacctg atcacacccg tcaaatgcaa tttcgatctc
     2101 ttttacttct ttggtatcaa caatttctaa tccaagcttt ttacatactt gtgctgtatg
     2161 atctgatggt gtcacaactt tgacatcttt ccttgcttct ttgatatctt ctgcaagata
     2221 actgatcgtc tctccaccac caagtccgat aatcccatga tctggcacaa gatcaagtgc
     2281 cgcttttgca cataaacgtt tcactttatt cctcctttgt ttttgcaaag ttttatttta
     2341 tgctttt
//
LOCUS       DAOMUE010000310         2346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_12972_length_2346_cov_1.962462, whole genome shotgun sequence.
ACCESSION   DAOMUE010000310 DAOMUE010000000
VERSION     DAOMUE010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12972_length_2346_cov_1.962462"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PLC71_10390"
     CDS             <1..578
                     /locus_tag="PLC71_10390"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HRM03087.1"
                     /translation="TKTLLGVSPIISIPYNICHIWHLNLGAITFVFYCFCVLIEAILK
                     GKEFKLYELLQIPMSLVTSVFINIFDRYLNIVPDTLGQKLLVLFFAIVVTGIGAATMV
                     NMKLVPNPADALAATIGDKLGKGMGIGKNVFDVTCFCTSGIIGLVFTRHIIGLGIGTV
                     LAVIFTGRVIAVYNYFLKHPMQNLAGITAEL"
     gene            602..859
                     /locus_tag="PLC71_10395"
     CDS             602..859
                     /locus_tag="PLC71_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HRM03088.1"
                     /translation="MLQKICRKLSKFMMSGLFGACLFMDGMPIRRKGLYGFVIFLIVC
                     MAGVFWLTDGKLEAVEQARKDMVHGIHELRYGKDQIPEEFR"
     gene            825..1406
                     /locus_tag="PLC71_10400"
     CDS             825..1406
                     /locus_tag="PLC71_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRM03089.1"
                     /translation="MEKIRFQKNSDEMMRVISEQKKSLYLKAYVGSVYKDGVWRKMPE
                     GQDPLQWFLTTSRTGNQMLYASAAVEAFRADGIPARYVEGYYLGASKIQDSKNGEVSI
                     TGENAHAWVEVYFDGVGWKAVDVTPGYYYNVATLQKMVNTPEQIKKNAAMILLGAVTV
                     LVIAGFILFVILEIRLWLLEQTLKKQYEQADMD"
     gene            complement(1539..>2346)
                     /locus_tag="PLC71_10405"
     CDS             complement(1539..>2346)
                     /locus_tag="PLC71_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM03090.1"
                     /translation="VNTGRDYHAAKRELDAASLSCDMICHSGACTYDVHGNGFHIASL
                     PKSIAKQILTVFERHGAFADIATEYGKTSITDKNTLLSYYKNEVFPAVEQEGKVYFRT
                     WHDFAMMVSKVRFFEGKESLLGCETPIYKISTTFFDQDKINALKDDIHGIDQLHAVST
                     SKNDLEITHVNAQKGTALLRYAQTKKITPSQILAVGDSGNDLSMLSLDLGVTAAMANA
                     SATVKDVCSVIAPSNDQDGVAFLIEDILEQNELAARVRRSFCLALDYSKI"
BASE COUNT          776 a          373 c          490 g          707 t
ORIGIN      
        1 atacaaaaac attgttagga gtatcaccga ttatatctat tccatataat atctgtcata
       61 tctggcattt aaacctagga gcaataacct ttgtgtttta ctgcttttgt gtgttgatag
      121 aggcaatctt aaaaggaaaa gaatttaaat tatatgaatt attacagatt ccaatgagtc
      181 ttgtgacaag tgtatttatc aatatttttg accggtattt aaatattgta ccagatacat
      241 tgggacagaa acttctggta ttattttttg caatcgttgt tacgggaata ggtgcagcaa
      301 cgatggttaa tatgaaactt gtaccaaacc ctgcagatgc acttgcagcg acgatcggag
      361 ataaacttgg caaaggcatg gggattggca aaaatgtttt tgatgttact tgtttttgta
      421 cgtcaggaat cattggactt gtatttacaa gacatatcat aggacttgga attggaactg
      481 tactggcagt tatttttaca ggaagagtca ttgcagttta taattatttc ctaaaacatc
      541 caatgcagaa tcttgcagga atcactgcag aattataagc cagagataat gaaagccctt
      601 tatgttacaa aaaatatgta gaaaattgtc gaaatttatg atgtctggat tgtttggagc
      661 gtgtctgttt atggatggaa tgccgatcag acgaaaaggc ttatatggtt ttgtaatctt
      721 tctgatcgtt tgtatggcag gtgttttttg gcttacggat ggtaaattag aagctgtaga
      781 gcaggcaaga aaggatatgg tgcatggcat tcatgaatta agatatggaa aagatcagat
      841 tccagaagaa ttcagatgaa atgatgcgtg taatatcaga acagaaaaaa tcattatatt
      901 taaaagcata cgtaggttcc gtatataaag atggagtctg gaggaaaatg ccagagggac
      961 aagatccatt gcaatggttt ttgacaactt cccgtacagg aaatcagatg ttatatgcat
     1021 cagcagcggt ggaagctttc cgtgcagatg gaattccagc aagatacgtg gaaggatact
     1081 atcttggagc atccaaaatc caggacagca aaaatggaga ggtatctatt acaggggaga
     1141 atgctcatgc atgggtggaa gtttattttg acggagttgg atggaaggca gtggacgtaa
     1201 ctccgggata ttactataat gtggcaacac ttcagaagat ggtaaacaca ccagaacaga
     1261 ttaagaagaa tgcagcaatg attttgttag gtgcggtaac agtgctcgta attgcaggct
     1321 ttattttgtt cgtgatctta gaaatccgtt tatggctctt agaacagact cttaagaaac
     1381 aatatgaaca ggcagatatg gattagaaga tcagaatctt acagatgcag atttttggat
     1441 tgttaggaat ctttggaatt gaaacaaaat ttggatggaa aacaaaagaa aagttaaaac
     1501 tgaggtatca atttcaaaaa tattgtatgt aatctaagct aaattttgga atagtccaat
     1561 gccaaacaaa aagaacgacg aactcttgca gccagttcat tttgctctaa aatatcttct
     1621 atcaagaacg caacgccatc ctgatcatta gaaggagcaa tgacagaaca tacatctttc
     1681 acagtagcag aagcatttgc catagcagca gtgactccaa gatcaagaga taacatagaa
     1741 agatcattcc cactatcacc aaccgcaagg atttgtgaag gagtgatctt ttttgtttgg
     1801 gcataacgaa gtagagctgt tcctttctgt gcatttacat gcgtgatctc cagatcattt
     1861 ttggaagttg ataccgcatg taactgatca atcccatgaa tatcatcttt taaagcattg
     1921 atcttatctt gatcaaaaaa tgtagtagag attttataaa ttggtgtttc acagccaagc
     1981 agactttctt ttccttcaaa aaagcgaact ttagaaacca tcattgcaaa gtcgtgccaa
     2041 gtacggaaat atactttacc ttcttgttca acggcaggaa acacttcatt tttataataa
     2101 gataataacg tatttttatc agtgattgag gtttttccat attccgttgc aatgtcggca
     2161 aaagcaccat gtcgttcaaa aacagttaag atctgttttg caatactttt tggaagtgaa
     2221 gcaatatgaa atccattgcc atgcacatca taagtgcaag ccccactgtg acagatcata
     2281 tcacaggaaa gagatgctgc atcaagttct cttttggcag cgtgataatc ccttccagta
     2341 tttaca
//
LOCUS       DAOMUE010000311         2335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_13057_length_2335_cov_2.357456, whole genome shotgun sequence.
ACCESSION   DAOMUE010000311 DAOMUE010000000
VERSION     DAOMUE010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13057_length_2335_cov_2.357456"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLC71_10410"
     CDS             complement(<1..187)
                     /locus_tag="PLC71_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03091.1"
                     /translation="MKIENKRQWVGELFFLLGTILYITYYFFYIAYYWYVEKDPTRQI
                     PVQRLFYIGLICFVVKII"
     gene            complement(270..1285)
                     /gene="galE"
                     /locus_tag="PLC71_10415"
                     /pseudo
     CDS             complement(270..1285)
                     /gene="galE"
                     /locus_tag="PLC71_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(1395..>2335)
                     /locus_tag="PLC71_10420"
     CDS             complement(1395..>2335)
                     /locus_tag="PLC71_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRM03092.1"
                     /translation="RRGIVGAIDLEQYSYTPEHEAKIRSTEKTVMERIPPRMKIRYQA
                     PIELPHVILLCDDWKNEVLEIVTEQKANLEKLYEFDLMQEGGHIAGWLVDGEVKEQFL
                     EKLRSYEEQMTEKYKDLSDDPMVYAVGDGNHSLATAKACYEKLKKNHQWEHIKDHPAR
                     YALVELENLHDDSQQFEPIHRVITGTDPEELIHALKTECCSEEGQTIRCYYGKKEEVL
                     HLNLHKHQLAVDKIQTFLDKYLKDNSGCIDYIHGEDVLKELSKEEQTIGIELPAMEKD
                     QLFPSVMTDGTLPRKTFSMGHACEKRYYIEGRKIQR"
BASE COUNT          601 a          515 c          346 g          873 t
ORIGIN      
        1 atattatttt gaccacaaaa cagattaatc caatataaaa taatctttgc actggaattt
       61 gtcttgtcgg atctttctct acataccaat agtaagctat ataaaaaaag tagtatgtaa
      121 tatagagtat tgttcctaat aaaaagaaca actctccgac ccattgtctt ttattttcaa
      181 tcttcatctc tattgctcct atttattttt caaaaaagcc atcgtatatt ctacgacagc
      241 ttttgtctaa tgctatgata atgaaataat taatcttctg ttctatatcc atccggattc
      301 atagactgcc atttccagga atctgcacac atttctttga ttcctttttc tgcagcccat
      361 cctaattctt cttttgcttt tgtcgcatca caatagcatg ttgcaatatc tccagctctt
      421 cgtggtttga tctcgtatgg aagtgtcttt ccacaagcct cttcatatgc atgtaagaca
      481 tctaatacgc tatatccgat tcctgttcca aggttgtaga tacgtacttc tggtttttct
      541 tcaaatttct ttaatgcctt cacatgtcct ttggcaaggt ctacaacatg gatataatca
      601 cggacacctg ttccgtctgg tgtgtcataa tcatcaccaa agactcctaa tttctcaagt
      661 ttaccaactg ctacctgtgc aatatatggt actaagttat ttggaattcc ctttggatct
      721 tctccgatca gtccgctttc atgtgctccg atagggttaa agtaacgaag taatacgaca
      781 ttccattctg gatctgcgac atggaaatct gttaagatct gctctaacat cccctttgtc
      841 tgtccatatg ggtttgtgat ctgtcctttt gggcattcct ctgtaattgg aatgaaagct
      901 ggatctccat agactgttgc tgaagaacta aagataatat tcttcacccc atgatttctc
      961 atcacatcac ataagatcag tgttcctgtg atattgttat gataatattc taatggtttc
     1021 tgtacagatt ctccaactgc ttttaatcct gcaaagttga tcacagaatc aatatcttct
     1081 gcatcaaaaa tcttctctaa tgcttcacgg tctaaaacat ctgcctcata aaattttact
     1141 gttttccctg tgatctgctc tactctcttt aaagcttctt cactagaatt atataaatta
     1201 cgacaacttc atatcctgca tttaaaagtt ccacacatgt atggctgccg atatatccag
     1261 caccacctgc tactaaaata gccatgatct ctcctccatt attgtatata ttatttattt
     1321 acactatttt ttatcatacc acatttcacg atatcattaa agtttttctt gtaaagtaat
     1381 tattgggcac tcgtctatct ctggatcttt cgtccttcaa tataataacg tttttcacaa
     1441 gcatgtccca tggaaaatgt ctttcttggc agtgttccgt ctgtcatcac acttggaaac
     1501 agctgatctt tctccattgc tggaagttca attcctatcg tctgctcttc ttttgacagt
     1561 tcttttaaga catcttctcc gtggatataa tcaatacatc ctgaattgtc tttgagatat
     1621 ttatctaaaa atgtctgtat tttatcaact gctaattgat gtttatgtaa attcaaatgg
     1681 agaacttctt ccttctttcc ataatagcag cgaattgttt gtccttcttc gctgcaacat
     1741 tctgttttta atgcatgtat taattcttct ggatctgttc cagtaatgac tctgtgaatt
     1801 ggttcaaact gctgggaatc atcatgtaga ttctcgagtt ctaccaatgc atatctcgct
     1861 ggatgatctt tgatatgttc ccattgatga tttttcttta acttctcgta acaggctttt
     1921 gctgttgcca gagaatggtt tccgtctccg actgcataga ccattggatc atcacttaaa
     1981 tctttatatt tttctgtcat ttgttcttca tatgatcgta gtttttctaa aaattgttct
     2041 ttgacctctc cgtctaccag ccatcctgcg atatgtccac cttcctgcat cagatcaaat
     2101 tcatatagtt tctcaagatt cgccttctgc tctgtcacga tttctaatac ttcatttttc
     2161 cagtcatcgc atagcaagat cacgtgtggc aattcaatcg gtgcctgata acgaatcttc
     2221 attcttggtg ggattcgctc catgactgtt ttctctgtgg aacggatctt tgcttcgtgt
     2281 tctggcgtat aactatattg ttccaaatcg atcgcaccta cgatccctct tcgaa
//
LOCUS       DAOMUE010000312         2291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_13357_length_2291_cov_2.221377, whole genome shotgun sequence.
ACCESSION   DAOMUE010000312 DAOMUE010000000
VERSION     DAOMUE010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13357_length_2291_cov_2.221377"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2245
                     /locus_tag="PLC71_10425"
     CDS             <1..2245
                     /locus_tag="PLC71_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRM03093.1"
                     /translation="SIHKNYLKAGADFITTNTFGCNRLKMEEAKYEAKDMLLAAVENA
                     RAARTEAGREDDSYIVLDIGPIGQLLEPMGTLTFDEAYDIILEQVETVKDQVDLVLFE
                     TMSDLYEVKAGVLAVKEHTDLPVFVTMTFEQNGRTLSGNDPETFINVAEGLGVDALGV
                     NCSLGPDELKPIIDEILEKASIPVMLQPNAGLPCLEHGETHYHVTPEEYVESMKDYMA
                     RGAAIVGGCCGTTPEFIGKLAEAAPKAVAERTVEKKTRVSSQTQTVTFGDHVIVCGER
                     LNPTGKKKMKKALLEERYDELVVEAVKQVEAGAEVLDVNVGLPGIDEPETMKRVVRLL
                     QEVVTLPLQIDSSEADAIENACRYYNGKPLINSVNGKDEVMEKIFPIAKKYGGVVIGL
                     TLEDGIPLKAEERFEIAKKIVNKAAEYGIGKENIIIDCLTLTASAQQKEVKETLRAIK
                     MVKKELGVHTVLGVSNVSFGLPNRPLLNRTFLALAMEAGLDLPIINPLDAELMGTIDA
                     FHVLFYKDVDSQTYIKNQSKDKETKPAAAAATTNFTLKDVIIHGLKDEVEKVTKEELK
                     DKEALTVINEVIIPALNIVGKDYETGKIFLPQLIQSAETTKKAFEVVKETFSANDGEE
                     KGPIIIATVEGDIHDIGKNIVKVVLESYGYKIIDLGKDVKVEKVVEAYKKYKPKAIGL
                     SALMTTTVASMERTIKALHEAGCSEPIWVGGAVVTEDIAHNIGADYYTEDAMAAVNLL
                     ENEILIL"
BASE COUNT          840 a          361 c          552 g          538 t
ORIGIN      
        1 atcgattcat aaaaactatc tgaaagcggg agcagacttt attacaacaa atacatttgg
       61 gtgtaaccga ttaaagatgg aagaagcaaa atacgaggcg aaagatatgc tgttagcagc
      121 agtagagaat gcaagagctg caagaacaga agctggaaga gaagatgact cttatattgt
      181 tcttgatatt ggaccgatcg gtcagttgtt agagccaatg ggaacattga cattcgatga
      241 agcatatgac atcatcctcg agcaggtaga aacagtaaaa gatcaagtag atcttgtact
      301 gtttgagaca atgagtgatc tatatgaggt gaaagctgga gttctggcgg tcaaagaaca
      361 cacagacctt ccagtgtttg ttacaatgac cttcgagcaa aatggaagaa cgttatctgg
      421 aaatgatccg gaaacattta ttaatgttgc agagggatta ggagttgatg cattgggagt
      481 taattgttct ttaggacccg atgaattaaa accgatcatt gacgaaatct tagaaaaagc
      541 aagtattcca gtgatgttac agcccaatgc cggacttcca tgtttagaac atggagaaac
      601 acactatcat gtgacaccag aagaatatgt ggaatctatg aaagattata tggcaagagg
      661 tgcagcaatc gttggtggat gctgtggaac aacaccagaa tttataggaa aacttgcaga
      721 agctgcgcct aaagcagttg cagagagaac agttgagaag aaaacaagag tatccagtca
      781 gacacagaca gttacgtttg gagatcatgt gatcgtctgc ggagaacgtc taaacccaac
      841 gggtaagaaa aagatgaaaa aagcgttgtt agaagagcgc tatgatgaac ttgtggtgga
      901 agcagttaaa caggtagaag ctggagcgga agtactagat gttaatgttg gacttcctgg
      961 aattgacgag ccagagacaa tgaaacgggt tgtacgtctg ttgcaggaag ttgtcacact
     1021 tccattacag attgacagtt ccgaagcaga tgcgattgag aatgcctgcc gttattataa
     1081 tggaaaacca ttgatcaact ctgtaaatgg taaagacgaa gtcatggaaa aaatcttccc
     1141 gattgctaag aaatatggag gagttgtgat cggactgact ctggaagacg gaattccgct
     1201 gaaagcagaa gaacgatttg aaatcgccaa gaagattgtc aataaagcag ctgaatacgg
     1261 aatcggtaaa gagaatatca tcatcgactg tctgacatta acagcatctg cacagcaaaa
     1321 agaagtaaaa gaaacactcc gagcgatcaa gatggtaaaa aaagaattag gtgtccatac
     1381 cgtattaggg gtatcgaatg tatcctttgg attgccaaac cgtccgttat taaatagaac
     1441 atttttagca ttagcaatgg aagcaggatt ggatttaccg atcatcaatc cgttagatgc
     1501 agaattgatg ggaaccatag atgcatttca tgtgctgttc tacaaagacg tggatagtca
     1561 gacttatatt aaaaaccagt ccaaagataa agaaacaaaa ccagccgcag cggcggcaac
     1621 aacaaacttc acattaaaag atgtgatcat tcacggatta aaagatgaag tagagaaagt
     1681 cacaaaagaa gaactgaaag ataaagaagc actgacagta attaatgaag tgatcatacc
     1741 agcgttaaat atcgtaggaa aagactatga gacaggaaag atcttcctgc cacagttaat
     1801 tcagtcagca gagacaacga agaaagcatt tgaagttgtt aaagaaacat tttcagcaaa
     1861 tgatggagaa gaaaaaggac cgatcatcat agcaaccgta gaaggtgata ttcatgatat
     1921 tggaaagaac atcgtgaaag ttgtactgga aagttatgga tataagatca tcgaccttgg
     1981 aaaagacgtt aaagttgaaa aggtagtaga agcttataag aaatacaagc caaaagcgat
     2041 tgggctaagt gcattaatga caacaaccgt tgcatcaatg gaaagaacga tcaaagcgct
     2101 tcacgaagca ggatgcagtg aaccaatctg ggttggggga gctgttgtga cggaagatat
     2161 cgcacacaat atcggagcag attattatac agaagatgcg atggcagcgg tgaatctgct
     2221 ggaaaatgaa attttgattt tgtagttggc caactgtaga ttttgagtat atgataaaaa
     2281 ataaataaac g
//
LOCUS       DAOMUE010000313         2288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_13389_length_2288_cov_2.512763, whole genome shotgun sequence.
ACCESSION   DAOMUE010000313 DAOMUE010000000
VERSION     DAOMUE010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13389_length_2288_cov_2.512763"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /gene="xylB"
                     /locus_tag="PLC71_10430"
     CDS             complement(<1..1331)
                     /gene="xylB"
                     /locus_tag="PLC71_10430"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HRM03094.1"
                     /translation="MLYIGVDLGTSAVKLLLMDEKGGIKKIVSKEYELHFPHPGWSEQ
                     KPEDWYSQSMEGIKELISECDKSQVAGISFGGQMHGLVVLDEDDDVIRPAILWNDGRT
                     QKETDYLNNEIGKDKLSEYTANIAFAGFTAPKILWMRENEPDNFNKIKKIMLPKDYLA
                     YKLSGTFCTDYSDASGMLLLDVKNKCWSKQMMEICGVSEEQLPKLFESYEVVGTLKED
                     IAEELGLSKEVKIIAGAGDNAAAAVGTGTVGDGMCNISLGTSGTIFISSKTFGVDSNN
                     ALHSFDHADGYYHLMGCMLSAASCNKWWMDEILKTKEYSKEQEGITKLGENHVFYLPY
                     LMGERSPHNDPKARATFIGMSMDTTREEMTQAVLEGVAFGLRDSLEVARSLGIKIERT
                     KICGGGAKSPLWKKIIANVMNLKVDVIESEEGPGYGGAMLAAVGCGEYDSV"
     gene            complement(1346..1840)
                     /locus_tag="PLC71_10435"
                     /pseudo
     CDS             complement(1346..1840)
                     /locus_tag="PLC71_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1868..1981)
                     /locus_tag="PLC71_10440"
                     /pseudo
     CDS             complement(1868..1981)
                     /locus_tag="PLC71_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4867 family protein"
     gene            complement(2024..>2288)
                     /locus_tag="PLC71_10445"
     CDS             complement(2024..>2288)
                     /locus_tag="PLC71_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM03095.1"
                     /translation="IFGYSPSYLSRMFQKYAGINYKDYLQAIRTEHGYRQLIETKMTI
                     SDISMENGFPNNKAFSKAFYKKYGIMPAEFRKRQKSAINEISN"
BASE COUNT          579 a          529 c          369 g          811 t
ORIGIN      
        1 tgtacgctgt cgtattctcc acatccaacg gctgcaagca tcgctcctcc atatcctgga
       61 ccttcttcgc tttcgatcac gtcgactttc agattcatca catttgcaat gatcttcttc
      121 cataatgggc tcttagctcc accgccgcag atctttgtgc gttcaatctt gattccaaga
      181 cttcttgcga cttccagaga atcacgaagt ccaaatgcaa ctccttctaa cacggcctgt
      241 gtcatttctt ctctcgttgt atccatggac attccgataa atgttgctct ggcttttgga
      301 tcattgtgtg gagatctctc tcccattagg tatggaagat agaatacatg gttttctcca
      361 agtttggtga ttccttcttg ttctttgctg tattcttttg tctttaagat ctcgtccatc
      421 caccatttat tgcaggatgc tgcacttaac atgcatccca tgaggtgata gtatccatct
      481 gcgtggtcaa aagaatgtaa ggcattgttg gaatctacac caaatgtctt actggaaatg
      541 aagatcgttc cagatgttcc aagggaaatg ttacacattc catctccaac agttccagtt
      601 cctaccgctg ctgccgcatt gtctccagct cccgcgatga tcttgacttc tttggataaa
      661 ccaagttctt ctgcaatatc ttcttttaat gttccgacaa cttcataact ttcaaacagt
      721 ttcggaagct gttcttcact cactccacag atttccatca tctgttttga ccagcattta
      781 ttctttacat ctaataataa cattccggat gcatcggaat aatccgtaca aaatgttcca
      841 gataatttat atgccagata atcttttggc agcatgatct ttttgatctt gttgaaattg
      901 tctggttcat tttcacgcat ccacaggatt ttgggtgccg taaatcctgc gaaagcgata
      961 ttagctgtgt actcagataa tttatctttt ccaatctcgt tatttaaata atctgtttct
     1021 ttctgtgtac gtccatcatt ccataggatt gcagggcgaa tcacatcatc atcttcatct
     1081 aaaacgacaa gtccatgcat ctgccctccg aagctgatcc cggcaacctg tgatttatca
     1141 cattcgctga tcaattcttt gattccttcc atagactgac tgtaccagtc ttctggtttc
     1201 tgctcagacc atcctggatg cggaaaatgc aactcatatt ctttggaaac gatttttttg
     1261 attccgcctt tttcatccat taacagtaat ttcactgcgg atgttccaag gtctacacca
     1321 atgtataaca tacaaacctc cgatcttatc cccaaggatt ttctgttgga tatctgactc
     1381 cagctggctg attgtatcca atctcttcta ctggaactcc aacatattta aatacttcat
     1441 ataatgtttt tccgaaatta ccaaatgcta cagctccatg atgagggaag tttttctcga
     1501 tctcaactcc taagttgtat aactgctgga ttggtgcatt gtatgctaag aagtttaatg
     1561 gacgaggccc aaaactgatg atctttaagc tgcttaatcc aacgacagct cttgcgattg
     1621 gtacaatttc agattataac ttgcattcag cataccacaa tatgcatctc cacgtccatc
     1681 taacagatta tctcctcttt cctctgctgc tgcaaagaac atttttggtc cttcaaaatg
     1741 tttggcaagt aatatttctg cgattcactg ataaactttc tgggaaacaa tctctgctga
     1801 ctgctacgat tccgatcttt acttttggca tattactcat ttgattctcc atcctttcct
     1861 tttttgatca ttttaatgtg atattttctc caactaatcc acaaaacgct ccaggtattt
     1921 gagtttcttc atgtgcgatc agccatttat tctgtgcagt taaaagtttg tcttctccat
     1981 ttatcactta taagattata acaatatgct tttgttttct cgactaatta cttatctcat
     2041 ttatggcact tttttgtctt tttcgaaatt cagcaggcat gatcccatac tttttataaa
     2101 aagctttaga aaatgctttg ttatttggaa atccattttc catggaaata tcactgatcg
     2161 tcatctttgt ctctatcagc tgtctgtacc catgttcagt gcggattgcc tgcaaataat
     2221 ctttataatt gattcctgca tatttttgaa acattcggga tagataagat ggagaatacc
     2281 cgaatatt
//
LOCUS       DAOMUE010000314         2274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_13504_length_2274_cov_1.882379, whole genome shotgun sequence.
ACCESSION   DAOMUE010000314 DAOMUE010000000
VERSION     DAOMUE010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13504_length_2274_cov_1.882379"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>657)
                     /locus_tag="PLC71_10450"
     CDS             complement(<1..>657)
                     /locus_tag="PLC71_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM03096.1"
                     /translation="LMVAVGIGTGVGTNALLARTLGQGNHKKAGKVAGNSLFLGGIIY
                     VVCLLFGIFGVKTYISSQTVDPQVISMGTSYLRICCIISMGIIFFSLFEKLLQATGRS
                     LYSTIGQVAGAVVNIILDPIMIYGIGPVPEMGVQGAAYATVIGQVVSTVLLFIFHMKL
                     NKEFEHGAKYMKPDAGIIKEIYAIGLPAIIAQALMSIMVYVMNLILKFSPSAQTAYGL
                     "
     assembly_gap    658..667
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<668..853)
                     /locus_tag="PLC71_10455"
     CDS             complement(<668..853)
                     /locus_tag="PLC71_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005333924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRM03097.1"
                     /translation="MAESNKMKDMPVNKLMIQMGIPMILSMALQAVYNIVDSAFVGNM
                     RAGSEAALNALTLVFPVQ"
     gene            complement(1246..1560)
                     /locus_tag="PLC71_10460"
     CDS             complement(1246..1560)
                     /locus_tag="PLC71_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HRM03098.1"
                     /translation="MKRCKITAIRKTEYKDLMEKYENPMENACDIEEGQVFIAEGWKR
                     PEGFCESAWESISPFVMTLVYGGSDIYEGWMKNKKSAMISCNDGFRPVSFYIEVLEDE
                     RS"
     gene            complement(1690..1845)
                     /locus_tag="PLC71_10465"
     CDS             complement(1690..1845)
                     /locus_tag="PLC71_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03099.1"
                     /translation="MRDELAPDANIRHRPLLLDEIDKYVAVIGDASKVSTITNAVYEG
                     YHVALDI"
     gene            1977..>2274
                     /locus_tag="PLC71_10470"
     CDS             1977..>2274
                     /locus_tag="PLC71_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM03100.1"
                     /translation="METPGRKHKGKNQNDPMRFVKKTSVTPDGEIAEKKVYDIDLEQI
                     KKEEMYDGFYAVITNLEGDIREIININKQRWEIEENFRIMKTDFEARPVYVRRED"
BASE COUNT          707 a          489 c          341 g          727 t
ORIGIN      
        1 acaatccgta ggctgtctga gcggaaggac tgaatttcaa aataaggttc attacatata
       61 ccataattga catcaatgcc tgtgcaatga tcgccggaag tccgattgca tatatttctt
      121 tgataattcc agcatctggc ttcatatact ttgctccatg ttcaaattct ttgtttaatt
      181 tcatatgaaa gataaacaat aacactgttg acactacctg tccgatgact gttgcataag
      241 ctgcaccttg aacccccatt tctggaacag gtccaatccc atagatcata atcggatcaa
      301 ggatgatatt taccactgct cccgcaacct gaccgattgt tgaatagagc gaacgccctg
      361 ttgcctgtaa caacttttca aatagagaaa agaaaatgat tcccatggaa atgatacagc
      421 atatcctaag ataacttgtt cccatagaga ttacttgcgg atcaaccgtc tgtgatgaaa
      481 tgtatgtttt aactccaaag attccaaaga gcaaacagac cacataaatg atcccgccta
      541 aaaacaaact gtttccagca accttacctg cttttttatg atttccctgt ccgagtgttc
      601 ttgcaagaag tgcattggtt cctactcctg ttccaatccc taccgcaacc atcagcannn
      661 nnnnnnnctg aactggaaat acaagcgtca aggcatttag ggctgcctcg cttcctgctc
      721 tcatgttacc tacaaaagca ctatctacaa tattgtagac tgcctgcaat gccattgata
      781 agatcattgg aattcccatc tggatcatga gcttattgac tggcatatcc ttcatcttgt
      841 tactttctgc catttttaat ttttcctcct tttttgcata aaaaaagaat gtagttcatc
      901 agaataagct ggtcttacgc tgattggcta cacacttttt aatgacattt tatgattgta
      961 aaaatagaaa aacgagcagc aaaaactgga tttacgctcg tttgctactc gtttttatta
     1021 tattttgatt ttttgaaaaa gtcaaatgaa aattattgta cattctcata attacaaatc
     1081 tggattgccg caccatctca ttttgtcctt ctaatctgtt ctgccataag tgcctccgcc
     1141 tttccttgtt tctcaatgtt gtattataat ctgtcccata ttaatttaac atctaaaccc
     1201 catatttata atcaatagta ccatattcct tcttaaaata aaactctagc ttctttcatc
     1261 ttccaacact tcgatataaa aactaaccgg gcggaatcca tcattacagc tgatcattgc
     1321 tgatttttta tttttcatcc atccttcata aatatcgctg cctccataaa ctaatgtcat
     1381 aacaaacgga gagatactct cccatgcact ttcacaaaat ccttctggtc gtttccatcc
     1441 ttctgcaata aatacttgcc cctcttctat atcgcaagca ttctccatag gattttcata
     1501 cttttccatt aagtctttat attctgtttt gcggattgca gtaattttac atcgtttcac
     1561 ttatttcttc cccttacatt ttattctatc aatttttatt ctcattatag cacaaaataa
     1621 aggcaccaga tatttctaaa aaacatccaa tgcctttatg atttttctta taaatatgtt
     1681 tttatttctc tagatatcta acgccacatg atacccttca tacacagcat ttgtaatcgt
     1741 agaaactttc gatgcatctc cgatcactgc cacatattta tcaatttcat ctaataaaag
     1801 tggtctatgt ctgatgttcg catctggagc aagttcatct ctcatttcta caatataaaa
     1861 aaatccctgt tgtcactgag aacagagatg aaaatctgat catcacttat tctccgaaat
     1921 ataaagctta tcagcgaaag atccgttccc gtcagatcga acgtgcaaaa aagatcattg
     1981 aaacacctgg ccgaaaacat aaagggaaaa atcagaacga tcccatgcgt tttgtcaaaa
     2041 aaacatccgt aacaccggat ggcgagatcg cagagaaaaa agtatatgat atcgatctgg
     2101 aacagatcaa aaaagaagaa atgtatgatg gattttatgc tgtgatcaca aatctggaag
     2161 gtgatatccg tgagatcata aatatcaata aacagcgatg ggagattgaa gagaatttca
     2221 ggatcatgaa aacagatttt gaagctcgtc ctgtgtatgt aagaagagaa gatc
//
LOCUS       DAOMUE010000315         2213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_13989_length_2213_cov_2.553753, whole genome shotgun sequence.
ACCESSION   DAOMUE010000315 DAOMUE010000000
VERSION     DAOMUE010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13989_length_2213_cov_2.553753"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLC71_10475"
     CDS             <1..629
                     /locus_tag="PLC71_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HRM03101.1"
                     /translation="FTIPGYEDVKAGIVHWPLSVIRIQSKDEKRLIDLADHILKKWRG
                     YTDEEAYIFAETEGEPHNTITPIARKKGDMYELDLTLRNNITTEECPLGLYHPHNEYH
                     HIKKENIGLIEVMGLAVLPSRLKAEMEHLSQCLIKGEDIVSKEDLKKHAAWVEEIKGK
                     YTDINKGNVMDILKEEIGQVFVKVLEDAGVYKYNEEGRKAFDRFIAVL"
     gene            660..1670
                     /gene="galE"
                     /locus_tag="PLC71_10480"
     CDS             660..1670
                     /gene="galE"
                     /locus_tag="PLC71_10480"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRM03102.1"
                     /translation="MKILVLGGAGYIGSHTVYELIDAGEEVVIIDNLETGHIEAVHPK
                     AKFYKGDLRDKDFVDSVLDQEKDIDAVIHFAANSLVGESMVNPLKYYDNNLCGTKTMV
                     QSLVEHGIDKIVFSSTAATYGEPEKVPIVETDRTEPTNTYGETKLSMEKMFKWVGRAH
                     GLRYVSLRYFNACGAHVSGQIGEDHNPETHLIPLILQVPNGKREAISIFGTDYDTKDG
                     TCVRDYIHVTDLAQAHILAVKYLMEGNESNIFNLGNGVGFTVKEVIETARKVTGKPIK
                     AVEEGRRAGDPAVLIASSEKAKNILGWKPEHADLEEIIASAWKWHSSHPQGYCSVQKE
                     DK"
     gene            1833..>2213
                     /locus_tag="PLC71_10485"
     CDS             1833..>2213
                     /locus_tag="PLC71_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HRM03103.1"
                     /translation="MKRLLGYLKEHLCVTILAPLFKMLEASFELFVPLVVASMIDVGI
                     GHHDIGYLWKMGGLLILLGIIGFSFSITAQYFAARSAVYTGKSMRSDLFAHMNQFSYQ
                     EIDQVGTSTLINRMSNDINQVQNGV"
BASE COUNT          781 a          352 c          495 g          585 t
ORIGIN      
        1 aatttacaat cccaggatat gaagatgtca aagcaggaat cgttcactgg ccattatctg
       61 tgatccgtat tcagagtaaa gatgaaaaac gcctcattga tcttgctgat catattttga
      121 aaaaatggcg tggatacacg gatgaagaag catatatttt tgctgagaca gaaggagaac
      181 cacataatac gatcacaccg atcgcacgta aaaaaggtga tatgtatgaa ctggatctga
      241 cacttcgtaa taatatcaca actgaagagt gcccattagg actttaccat ccacacaatg
      301 agtatcatca tatcaagaaa gaaaatatcg ggctgatcga ggttatggga cttgctgttc
      361 tgccatctag attaaaggca gagatggaac atttaagcca gtgtctgatc aaaggagaag
      421 atattgtttc taaggaagat ttaaagaagc atgcagcatg ggttgaagaa atcaaaggaa
      481 aatatacaga tatcaataaa ggaaatgtca tggatatctt aaaagaagag atcggacagg
      541 tatttgttaa agttcttgaa gatgccggag tttataaata taatgaagaa ggaagaaaag
      601 cattcgaccg atttattgca gttttataat gacaaggtca tagaaaggaa agcaagacaa
      661 tgaaaatatt agtattaggc ggagctggat acataggttc tcatacagta tacgaactga
      721 tcgatgctgg agaagaagtc gtgatcatcg ataatctgga aacaggacat atcgaggctg
      781 ttcatccaaa agcaaagttt tataaaggtg acttaagaga taaagatttt gtagatagtg
      841 tactagatca ggaaaaagat atcgatgcag tcattcattt tgcagcaaac tccttagttg
      901 gagaaagtat ggtaaatcca ttaaaatatt atgataataa cctttgtgga acaaagacaa
      961 tggtacagtc cttagtagaa catggcatcg ataagatcgt attctcctca acagcagcaa
     1021 cttatggaga accagaaaag gtaccgatcg tggagacaga ccgcacagaa cctaccaaca
     1081 cttatggaga gacaaaactt tctatggaaa aaatgtttaa atgggttgga cgcgctcacg
     1141 gacttcgtta tgtatcttta agatacttta acgcctgcgg agcacatgta agtggacaga
     1201 tcggagaaga tcataatcca gaaacacatc tgatcccatt gatcttacag gtgccaaatg
     1261 gcaaaagaga agcaatcagt atctttggaa ctgattatga tacaaaagat ggaacttgtg
     1321 taagagacta tattcatgta acagatcttg cacaagcaca tattctggca gtgaaatact
     1381 taatggaagg taatgaaagc aacatcttca atcttggaaa tggtgttgga tttactgtaa
     1441 aagaagtcat cgaaacagcc agaaaagtga caggaaagcc gatcaaggct gtggaagaag
     1501 gaagaagagc tggagatccg gctgtcttga tcgcttccag tgaaaaagcg aagaatatcc
     1561 ttggctggaa gccagaacat gcagatcttg aagaaatcat tgcatccgca tggaaatggc
     1621 acagcagcca cccacaagga tattgttctg ttcaaaagga agacaaataa ctctttttga
     1681 accataaatc atccccgtac cgtttagagc atgttacctg gctctaagcg gtactttttt
     1741 gttttcgtaa aatatctggg actatgttca aataaaaaat aaaatggtgt atactaaaga
     1801 aaatagtaag ataaaagaag gagtacgaat cgatgaaacg gttactagga tatttaaagg
     1861 agcatctttg tgttacgatt cttgcaccat tatttaagat gctggaagct agttttgaac
     1921 tatttgtacc attggtcgtt gccagcatga tcgatgtagg aatcggacat cacgatattg
     1981 gatatttgtg gaagatggga ggattattga tcttacttgg aatcattgga ttttcatttt
     2041 ctatcacagc gcagtatttt gcggcaagat cggctgtgta tacaggaaag tcgatgagaa
     2101 gtgatttgtt tgcacatatg aatcaatttt cttatcagga gatcgatcag gttgggacat
     2161 caacgctgat caaccgcatg agtaatgata tcaatcaggt gcaaaatgga gtt
//
LOCUS       DAOMUE010000316         2213 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_13991_length_2213_cov_2.215477, whole genome shotgun sequence.
ACCESSION   DAOMUE010000316 DAOMUE010000000
VERSION     DAOMUE010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13991_length_2213_cov_2.215477"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PLC71_10490"
     CDS             complement(<1..820)
                     /locus_tag="PLC71_10490"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRM03104.1"
                     /translation="MGENRIKLVAADMDGTLLNRERKITKYTQNVIKKAMAQGVYFVP
                     ATGRAVNALPPELKEMEGIRYGIFSNGATIYDLKEEKVIYKNHFEMDRVLELMDFLKQ
                     FDLMESISLNGQSYANKKEMENIDYYELDSNTKAIVQGSRVLVDDLEMFVKEQSTTVE
                     KTTLLFKTMEERRRAADALSKIEDIQFSSSLPKNLEISKKGCNKGDGLFHLAKILNIQ
                     KEEIMSCGDADNDRELLESAGLAVVMENGLDSMKAIADYITVSNQENGVAKAMEK"
     gene            complement(807..1526)
                     /gene="trmD"
                     /locus_tag="PLC71_10495"
     CDS             complement(807..1526)
                     /gene="trmD"
                     /locus_tag="PLC71_10495"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRM03105.1"
                     /translation="MNRFHILTLFPEMVMDGLNTSIIGRAIEAGLLEINAVNIRDYST
                     NKHMKVDDYPYGGGAGLVMQPEPVYRAYKDLEKDMKKKPRVVYLTPQGTTFHQEMAKE
                     LAKEEELVFLCGHYEGIDERVLEEIVTDYVSIGDYVLTGGELPAMVMIDSISRLVPGV
                     LHNDDSAGDESFENGLLEYPQYTRPPVFLDKEVPEVLLSGHHENIRKWRHEQSVKRTK
                     ERRPDLWEAYEKEMKEKDGGK"
     gene            complement(1519..2034)
                     /gene="rimM"
                     /locus_tag="PLC71_10500"
     CDS             complement(1519..2034)
                     /gene="rimM"
                     /locus_tag="PLC71_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393633.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRM03106.1"
                     /translation="MEEFLQVGAIANTHGIAGEVKVFPMTDDIKRFKKLKEVYLDTGK
                     ERKLLHVVSCKFVKNQPVLRFKEFSNINEVEMYKRKGLFVTRDQAVPLEKDEYFIADL
                     IGLSVIREDNGEVLGELTDVLQTGANDVYEVKMEDGKEVLLPAIRECIKNVDLQKGEI
                     TVHVMKGLLDE"
BASE COUNT          608 a          520 c          288 g          797 t
ORIGIN      
        1 atttctccat tgcctttgcg actccatttt cctgattgga aaccgtaata tagtctgcga
       61 tcgctttcat agaatcaagg ccattttcca taacaaccgc aagtcctgca ctttctaaaa
      121 gttctctgtc attgtctgca tccccacaac tcataatctc ttctttttga atattcaaga
      181 tctttgcaag atggaacaga ccatctcctt tattacagcc tttcttagaa atctcaagat
      241 tctttggaag agaacttgaa aactgaatgt cttcaatctt actcaatgca tccgctgctc
      301 ttcgtctttc ttccatcgtc ttaaataata aagttgtctt ctcaacagtt gttgactgtt
      361 cttttacaaa catctctaaa tcatcaacaa gaactctcga cccctgcaca attgcttttg
      421 tatttgaatc aagttcataa taatcaatat tttccatttc ttttttgtta gcataggatt
      481 gcccatttaa actaatactt tccattagat caaattgctt aagaaaatcc atcaactcta
      541 aaactcgatc catttcaaaa tgatttttat aaatcacttt ttcttctttt aagtcataaa
      601 tggttgcacc attactgaaa atcccatagc gaattccttc catctctttt aactctggcg
      661 gtaatgcatt cacagctctt cctgtagccg gaacgaaata aacaccttgt gccattgctt
      721 ttttgatcac attctgtgta tactttgtaa tcttacgctc tcgattcaac agtgttccat
      781 ccatatccgc tgcgacaagc ttaatcctat tttcccccat ctttttcttt catctccttc
      841 tcatatgcct cccatagatc cggtcttctc tctttggtac gtttgactga ctgttcatgt
      901 ctccatttgc ggatattctc gtgatgtccg gataaaagaa cttctggcac ctctttatct
      961 aaaaacactg gtggtcttgt atactgtgga tattcaagca acccattttc gaaagattca
     1021 tccccagcgg aatcatcatt atgtaaaaca cctggaacca atcttgaaat tgaatcgatc
     1081 atcaccattg caggcaattc cccaccagtc aatacataat ctccgattga tacataatct
     1141 gtaactattt cttccaaaac cctctcatcg attccttcat aatgcccaca taaaaaaact
     1201 aattcttctt cttttgcaag ttcctttgcc atctcctgat gaaatgttgt cccttgcggc
     1261 gtcagataga caactcttgg tttcttcttc atatcttttt caaggtcttt atacgcacgg
     1321 taaaccggct ctggctgcat cacaagtccg gctccgcctc catatgggta atcatccact
     1381 ttcatatgtt tattggtaga atagtcacga atattgaccg cattgatctc aagaagtcct
     1441 gcttcaatcg ctcttccgat gatacttgta ttcaacccgt ccatcaccat ctctggaaat
     1501 aatgttaata tatgaaatct attcatctaa taatcctttc atgacatgga ctgtgatctc
     1561 acctttttga agatccacat tcttaatgca ttctctgatc gctggaagta aaacttcttt
     1621 tccatcttcc atcttcactt catagacatc atttgcaccc gtctgtaaaa catcagttaa
     1681 ttccccaaga acttctccat tatcctctct gatcacagaa agaccgatca gatctgcgat
     1741 aaagtattcg tctttttcaa gaggtactgc ctgatctctt gtaacaaaga gcccttttct
     1801 tttatacatc tctacttcgt tgatatttga aaattcttta aatctcaaaa ccggctggtt
     1861 ctttacgaat ttgcatgaca ctacgtgtaa tagttttctt tcttttcctg tatctaaata
     1921 tacttctttt aatttcttaa atcgtttgat atcatctgtc atcggaaata ctttgacttc
     1981 cccagcgatg ccgtgtgtgt tggcgattgc tcctacttgt aagaattctt ccatggattc
     2041 attttattcc tttcattttg atttttctat ttattattgt ataaaatggt ttaaaaaaac
     2101 acaactattt tattataatc gtacttgtta taatatatca atacaaaatg gcagaagatt
     2161 caactccaat aatattatca acaaactgaa gataagcaat ttgaatattt tcc
//
LOCUS       DAOMUE010000317         2206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_14055_length_2206_cov_2.085077, whole genome shotgun sequence.
ACCESSION   DAOMUE010000317 DAOMUE010000000
VERSION     DAOMUE010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14055_length_2206_cov_2.085077"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1650
                     /locus_tag="PLC71_10505"
     CDS             163..1650
                     /locus_tag="PLC71_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRM03107.1"
                     /translation="MRGIQTPVRNVRRRIFKEIAKFGYEQRNLQDLEDLPYEIVPGEV
                     AKYRDSIYRERAIVGERLRLAMGMSLNPADKPTRITKGIDESNISEKYYEPPLLQVIP
                     SACNECKEKAFIVGEQCQGCMAHPCMEVCPKKAISFKDGYSYIDQEKCIKCGQCKKVC
                     PYGAIYERKRPCANACGVGAIETDYAGRAKINPDKCVSCGMCMVNCPFGAIADKSQIY
                     QLIKAMNEGAEVIAILAPAFVGQFGPKATPNKIKAALLDIGFADVWEVAVGADAGALE
                     EAKHYVQSVATGKLPFLLTSCCPSWSMLGKKYFPEVIDEISNALTPMVATARAARIAS
                     PNAKIVFVGPCVAKKLEASRRTVRSEVDFVITFEELAGMFDAKEIDFNTYEKEEELND
                     AFGAGRGYAVASGVAGAIKACIDEYYPGTEVKIDHVEGLADCKKMLLQAKAGKKKGYL
                     IEGMGCPGGCVAGAGTNIPVMKAQIQVKKFVKEAEDFVPPETAQY"
     gene            1799..>2206
                     /locus_tag="PLC71_10510"
     CDS             1799..>2206
                     /locus_tag="PLC71_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRM03108.1"
                     /translation="MAAKKTRKRKTSSAKKRTRTRQKDFAPFYKEIAVLVVFALFIFL
                     FLSNFGICGYIGNSISTFFFGAFGTTHYIIPAVSFILAILLISNDYSSLAVKKTCFAI
                     LLLCMVSTIFQMIYNIEVRDAYSAFVFSGRTHRG"
BASE COUNT          759 a          316 c          527 g          604 t
ORIGIN      
        1 agattgattg aaataattaa tctgtctgaa aataaataga aaatttgaat aaaaaaacag
       61 ataatacctt tataatattc ctgttttggt atatgataag aataacaaat atgttaaaaa
      121 aaacacaaac tatcttaagg catcattaga ggagggaata tcatgagagg aatacagaca
      181 ccagttcgga atgtgagaag aagaattttc aaagagatcg caaagtttgg ttacgaacaa
      241 agaaacttac aggatcttga agatcttcca tatgagatcg tacctggaga agttgcaaaa
      301 tatcgagata gcatttacag agaacgagcg atcgtaggtg agagactgag gcttgccatg
      361 ggaatgtcat taaatccagc ggataaacca acaagaatta cgaaaggaat tgatgagagc
      421 aatatttcag agaagtatta tgagccgcca cttcttcagg tgattccatc tgcatgcaat
      481 gaatgtaagg agaaggcatt tattgtagga gagcagtgcc agggatgcat ggcacatccg
      541 tgtatggaag tttgtccgaa gaaggcaatt agttttaagg atggatattc ttatatcgat
      601 caggaaaaat gtatcaaatg tggacaatgt aaaaaggtct gtccatatgg tgcgatttat
      661 gaaagaaaac gtccttgtgc aaatgcctgt ggggttggag cgattgaaac agattatgca
      721 ggaagagcta agatcaatcc agataaatgt gtttcttgtg gtatgtgtat ggtaaattgt
      781 ccgtttggag caattgcaga taaatcccag atttaccagt tgatcaaggc aatgaatgaa
      841 ggtgcggaag tgatcgcgat cttagcacct gcatttgtcg gacaatttgg accaaaagca
      901 acgccaaata agattaaagc tgcacttctt gatattgggt ttgcagatgt gtgggaagtt
      961 gcagttggag cagatgcagg ggccttagaa gaggcaaagc attatgttca aagtgtagct
     1021 acagggaaac taccattctt gctgacatca tgctgcccat catggtctat gcttggaaag
     1081 aaatattttc cagaggtgat cgatgaaatt tctaatgcac taacaccaat ggtagcaacg
     1141 gcaagagcgg caaggattgc atctccaaat gcaaaaattg tttttgtagg gccatgtgtt
     1201 gccaaaaagt tagaagcatc cagacggaca gtgcgaagtg aggtcgattt tgtgatcaca
     1261 tttgaagaac tggctggaat gtttgatgcg aaagaaattg attttaacac atatgaaaaa
     1321 gaagaagagt taaatgatgc ctttggagca ggacgtgggt atgcggttgc gagcggagta
     1381 gctggagcga tcaaggcatg tattgatgaa tactatcctg gaacagaagt aaagatcgat
     1441 catgtggaag gtcttgcaga ttgtaagaaa atgcttttac aggcgaaagc aggaaagaaa
     1501 aaaggctatc tgatcgaagg aatgggatgt ccaggtggat gtgttgcagg agctggaacc
     1561 aatataccag ttatgaaagc acaaattcag gttaagaagt ttgtaaaaga agcggaagat
     1621 tttgtacctc cagaaactgc acaatattaa gaggttaaaa gaaaagggaa atgtatggga
     1681 gataaataca tttccctttt tctatggtgg gacttgcgta ttttgataat taattgtaaa
     1741 ataagaaaat acgcagatta tgtatacaaa atgtatacaa agagaggtaa caatttctat
     1801 ggcagcaaag aagacaagaa agaggaagac atcttctgca aagaaacgga cacgcacaag
     1861 acaaaaagat tttgcaccgt tttataaaga gattgcagta ttagttgttt ttgctctttt
     1921 cattttttta tttttaagta attttggaat atgcggatac ataggaaaca gcatcagtac
     1981 atttttcttt ggagcgtttg gaacgactca ttacatcatt ccggcggtaa gctttattct
     2041 ggcaatttta ttgatctcca atgattacag cagtcttgca gtgaagaaga catgttttgc
     2101 gatcctgctg ttatgtatgg tatccacaat ttttcagatg atatataata tagaagtaag
     2161 agatgcttat tcagcctttg tatttagtgg aagaacccac agagga
//
LOCUS       DAOMUE010000318         2199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_14121_length_2199_cov_2.253265, whole genome shotgun sequence.
ACCESSION   DAOMUE010000318 DAOMUE010000000
VERSION     DAOMUE010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14121_length_2199_cov_2.253265"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PLC71_10515"
     CDS             complement(<1..911)
                     /locus_tag="PLC71_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03109.1"
                     /translation="MQTLLMTMVLFSLAGALFLLIIFYDMRLEKWNWNVLVLLLLFFL
                     LNHLVYSFGQIDKGANYYEVLPVPIWVIWCIVGAIDFLLIWEGIRLKRISKEKVNRNS
                     IKEAMNLLPTGICYFNSDGTVKLANTQIYQLFRILAHKDLQEFSELRDALHTSIQNGV
                     IRLAGKKDTYIFPDGKVWDYRENEVRDRDGKIYIEAVFMDVTKQYHEKINLTKQTEKL
                     KEISRELRYLSDNVLILTREREVLAAKTKLHDQMGAGLTAIRQNLLQGSEDYSDAVRL
                     LCQAVNAIWNDNQQPFGEGEFEQFLQDA"
     gene            complement(911..1549)
                     /locus_tag="PLC71_10520"
     CDS             complement(911..1549)
                     /locus_tag="PLC71_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03110.1"
                     /translation="MMLLDRLKENRKTIEESNCLEQENYQTKAKIYMLREKNRLYDKL
                     QTQTAGQIGLLNELLIRYEEEEDLAESRRLLAKISLIGTYIKRYGNLIFIGEREAVSD
                     IAELGACLEESFSSLRLMGIECALTAPAGERIYVRDAVRIYSFFESILEACVDSIKFM
                     WVKIRPCGEEMVFCMEVESEDDLNSFFDKTDQGENEDGIWKFTFTIKKAGEK"
     gene            complement(1536..>2199)
                     /locus_tag="PLC71_10525"
     CDS             complement(1536..>2199)
                     /locus_tag="PLC71_10525"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HRM03111.1"
                     /translation="LFIPQAAVQMAVLLGQPEEYTLPKWSKLLYIPTTLCSLLVLTND
                     FHQLVFSFSAGEVWTDKGYSYAWGYYIVLLWDVICAVSAFVLMVYKCRSSRRKKYLPI
                     IGICISIIYAIIYASGAEWMQVIGGDITAALCLMFMCIFESCLHCGLIQTNTGYEQLF
                     EVCTMGAQITDQDYHVIYTSANAMKLSEMVMREAEKEEVRIDKKTMIKNRPIQGGHHD
                     VA"
BASE COUNT          655 a          570 c          304 g          670 t
ORIGIN      
        1 gcatcctgca gaaattgttc aaattctcct tccccaaaag gctgctgatt atcattccag
       61 attgcattta ccgcctgaca aagtaatctg accgcatcgc tgtaatcttc acttccctgc
      121 aataaattct gccggatcgc tgtcaatccc gcacccatct gatcatgcag ttttgtcttg
      181 gcagcaagca cttccctttc tcttgtcaag atcaatacat tgtcagaaag atatcgaagt
      241 tcccttgata tttctttcag cttctccgtc tgctttgtca aatttatttt ctcatgatat
      301 tgctttgtaa catccataaa aacagcttct atataaatct taccatctct gtctctgact
      361 tcattttccc gataatccca taccttccca tctgggaaaa tataagtgtc ttttttacct
      421 gcaagcctga tcacaccgtt ttgtatactt gtatgaagtg catctcgtaa ctcactgaat
      481 tcttgcagat ctttatgcgc aagaatccta aaaagctgat atatctgcgt attggcaagc
      541 ttcactgttc catcagaatt aaaatagcag atgcctgtcg gaagaagatt catcgcttct
      601 ttgatcgaat tccgattaac tttttctttt gatatccttt ttaatctgat gccttcccaa
      661 ataagcaaaa aatcaatggc accgacaata caccaaataa cccatatcgg aacaggcaac
      721 acttcataat agtttgctcc cttatcgatc tgcccgaacg aatataccag atgattcaac
      781 aggaaaaaca aaagtaaaag aactaaaaca ttccaattcc atttctccag gcgcatatca
      841 taaaatataa taagcaaaaa taaagcacct gccagactga acaacaccat cgtcatcaac
      901 aacgtctgca ttacttttca cctgcctttt ttatggtaaa tgtaaatttc catataccat
      961 cttcattttc accttgatcc gtcttgtcaa agaatgagtt cagatcatct tcactttcta
     1021 cttccataca aaaaaccatc tcttctccac acggacgaat ctttacccac ataaatttaa
     1081 tagaatctac acaggcttct aatattgatt caaaaaagct ataaatgcga accgcatccc
     1141 tcacataaat acgctcccct gctggggctg taagcgcaca ttctatcccc atcagcctca
     1201 agctgctaaa agactcctcc aaacaagctc caagctctgc aatatctgac acggcttctc
     1261 tttctccaat aaagatcaaa ttcccatatc tttttatgta tgttccgatc aaactgatct
     1321 tagccagcag tctgcggctc tctgccagat cttcttcctc ttcgtatcga atcagcaatt
     1381 cattcaacag cccaatctgt ccagcagtct gtgtctgcag tttgtcatat aagcggttct
     1441 tttcccgcaa catataaatc tttgcctttg tctgataatt ttcctgttcc agacaattac
     1501 tctcttcgat cgtctttcta ttttctttca atcgatcaag caacatcatg atgtccgccc
     1561 tgaatcggtc tgtttttgat cattgttttc ttatcaatcc taacttcttc cttctccgct
     1621 tccctcatca ccatttctga aagcttcatt gcattagcag aagtatatat gacatgataa
     1681 tcctggtctg tgatctgcgc tcccattgta catacttcaa atagctgttc atatcctgta
     1741 tttgtctgaa tcaacccgca gtgaaggcag ctctcaaata tgcacataaa catcagacat
     1801 aacgccgctg tgatatctcc accaataacc tgcatccact ctgcaccact tgcataaata
     1861 atggcataaa taatagaaat acaaatccct atgatcggca aatacttctt tctccgagat
     1921 gacctgcact tataaaccat taaaacaaat gccgatactg cacaaataac atcccacaac
     1981 agtacgatat aatatcccca cgcataactg taacctttat ccgtccatac ttcacctgcc
     2041 gaaaaagaaa acacgagctg atgaaaatca tttgtcaaaa caagcaagga gcaaagcgtt
     2101 gtaggaatat ataacaactt tgaccatttc ggtaaagtat actcttctgg ctgtccaaga
     2161 agaactgcca tctgtaccgc cgcctgggga atgaacaac
//
LOCUS       DAOMUE010000319         2187 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_14221_length_2187_cov_1.921670, whole genome shotgun sequence.
ACCESSION   DAOMUE010000319 DAOMUE010000000
VERSION     DAOMUE010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14221_length_2187_cov_1.921670"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLC71_10530"
     CDS             complement(<1..395)
                     /locus_tag="PLC71_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM03112.1"
                     /translation="MSKEYESKTEHKENLIDIAGLFDDLIKGIIQMKWRFLIILVIFG
                     VVFCGYKRLTYSPYYVASSTFTINSSITSENSNTYIENATANQMAKSFPYILKSGAFK
                     EVLAQDLGLQSVPGTIEAEVMENTNLFTL"
     gene            complement(388..1950)
                     /locus_tag="PLC71_10535"
     CDS             complement(388..1950)
                     /locus_tag="PLC71_10535"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRM03113.1"
                     /translation="MCSDKRNISVNDYEMNIDLYSILRDIWKYALVILLLAASMSLLS
                     YVYVGRNYHKQYESTSTFIVSSKGSNNSVYSDLSATSEMATKFSEILNSSILQKKVAK
                     EMDMDYFPGSASAEVVNETNLLVLKVQADSPEMAFRLNKAIMNNYSVVTDQLIGNVVL
                     DVLQKPTVPSGPVNKFQPTALMKKTFFTTIVALCGLIAILSFLKDTVRKPKEVSRKLD
                     AKLLQTLYHEKIYKTWKARIHRKKSPVLLTNPGTSFQYVEDMKKLARKVSSKMKEKNA
                     KTLLVASVEENEGKSTVAANLALALAEESEKVLLIDADLRKPSQYKIFGLDQEEIQQF
                     GEVLNGNEQIDNLVTDLPKSELLLIAGSMIYPNSTEMIASPIFQKIVEFFKTKLDYII
                     IDTPPMSQAADAEELVDLADASILVVRQHTALVKDINETISILNSAEGTMLGCVYNDV
                     FHGVAQTARNYGYKYAYGSGYGYGSKYGYGNHGYGYGYGYGYGYGSRTKKGNEDKTQE
                     SKTERQVKKDHE"
     gene            complement(1962..>2187)
                     /locus_tag="PLC71_10540"
     CDS             complement(1962..>2187)
                     /locus_tag="PLC71_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRM03114.1"
                     /translation="YTKKGDKLDAASYISKVCMGGDYDSDVPGDINKSKVKIKDEVDY
                     DTPGSYEITYSVKSGKSRTGTVRLIVIVTE"
BASE COUNT          584 a          487 c          343 g          773 t
ORIGIN      
        1 ttcaacgtaa acaggtttgt attctccatg acttctgctt cgatcgttcc aggaacagac
       61 tgaagaccaa gatcctgtgc caatacttct ttaaatgctc cacttttcaa aatatatgga
      121 aaagattttg ccatttggtt ggctgttgca ttctcaatat atgtattgga attctctgat
      181 gtaatactgg aattgatcgt aaatgtggaa gatgctacat agtatgggga ataggtaagt
      241 ctcttgtatc cacagaacac tacaccaaaa atcacaagaa taatcaaaaa tctccatttc
      301 atttgaatga tgcctttgat caagtcatca aacaatccag caatatcgat cagattctct
      361 ttatgttctg tttttgattc atattcttta ctcatgatct ttcttcacct gccgttctgt
      421 ttttgattct tgtgttttat cttcattgcc ctttttcgtt ctgcttccat aaccatagcc
      481 atagccgtaa ccgtaaccat atccatggtt tccataaccg tatttgcttc cgtaaccata
      541 accgcttcca tatgcatatt tgtatccata atttcttgct gtctgagcaa ctccatggaa
      601 tacatcgtta taaacgcatc caagcatcgt tccttctgct gaattcagga tactgatcgt
      661 ctcattgata tcttttacta atgctgtatg ttgtcttaca acaaggatcg atgcatctgc
      721 aaggtctact aactcttctg catcggccgc ctgtgacatt ggtggtgtat caataataat
      781 gtaatccagc tttgtcttaa aaaactctac gatcttctgg aagatcggtg atgcgatcat
      841 ctctgtagaa tttggataga tcatagaacc tgcgatcaat aacaattctg attttggaag
      901 atctgttacc agattatcta tctgttcatt tccattcaat acttctccaa actgctgtat
      961 ttcttcctga tccaatccaa aaatcttgta ttgtgaaggt ttgcgaagat ccgcatcgat
     1021 caacaatact ttctctgatt cttccgcaag tgccaatgct aaattggctg caactgttga
     1081 tttaccttca ttttcttcca cacttgcaac cagaagtgtt tttgcattct tctctttcat
     1141 cttactgctg actttgcggg caagtttctt catatcctcc acatattgaa agcttgttcc
     1201 tggatttgtc aacaataccg gactcttttt gcggtgaatt cttgctttcc atgtcttata
     1261 gatcttctca tgatataacg tctgcaaaag ctttgcatca agtttccttg aaacttcttt
     1321 tggttttctc actgtatcct ttaagaatga caagattgcg atcaatccac ataacgcaac
     1381 gatcgtcgta aaaaatgttt tcttcataag tgctgttggc tgaaatttat ttaccggacc
     1441 acttggcact gttggtttct gcaatacatc cagcacaaca tttccgatca gctgatctgt
     1501 cacaacagaa taattattca tgattgcctt attcaaacga aacgccatct ctggggaatc
     1561 tgcctgaact ttcaaaacta aaagattggt ttcatttact acctctgcag atgcacttcc
     1621 tggaaaataa tccatatcca tctcttttgc gactttcttt tgtaagatcg aactgtttag
     1681 aatctcagaa aactttgtcg ccatctcaga cgttgcactc aaatctgaat atacactatt
     1741 gttacttcct ttgctgctta cgataaatgt acttgttgat tcatactgtt tgtgatagtt
     1801 tcgtccgaca taaacataag agagcaagct catagatgct gccagaagca ggatcactag
     1861 tgcatatttc cagatatctc ttaagatact atacaaatca atattcattt cataatcgtt
     1921 gactgatata tttcgtttat cactgcacat ttgccggcct cctattctgt aacgatcacg
     1981 atcagtctta cggtacctgt acggctcttc ccactcttta cagaatatgt gatctcatag
     2041 gaacctgggg tatcgtaatc tacttcatct ttgatcttca ctttgctttt attaatatct
     2101 cctggtacat ccgagtcata atctcctccc atgcacactt tagatatgta agatgctgca
     2161 tctagtttgt cacctttctt cgtataa
//
LOCUS       DAOMUE010000320         2152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_14520_length_2152_cov_2.274678, whole genome shotgun sequence.
ACCESSION   DAOMUE010000320 DAOMUE010000000
VERSION     DAOMUE010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14520_length_2152_cov_2.274678"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..1598)
                     /gene="eno"
                     /locus_tag="PLC71_10545"
     CDS             complement(300..1598)
                     /gene="eno"
                     /locus_tag="PLC71_10545"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRM03115.1"
                     /translation="MNYLEIEKVVGREILDSRGNPTVEAEITLVDGTVARGTAPSGAS
                     TGEFEALELRDGDKERYLGKGVTKAVENINTKISEAIIGLDASDTYAVDKAMIDADGT
                     ADKSNFGANAILAVSIAAARAAATSLEVPLYRFLGGVSGNRLPVPMMNIVNGGCHALS
                     SGLDVQEFMIMPVGAPSFKECLRWCAEVFHALAAILKERGLATSVGDEGGFAPALKSD
                     EEAIETILEAVKKAGYEPGKDFKIAMDAASSEWKSEKGKGYYKLPKAGTEYTAEELIE
                     HWAALCEKYPIISIEDGLDEEDWEGWKKLTERLGDKVQLVGDDLFVTNTERLSKGIEM
                     GAGNSILIKLNQIGSISETLEAIKMAHKAGYTAVTSHRSGETADTTIADLAVALNTCQ
                     IKTGAPSRSERVAKYNQLLRIEEALGDSAVYPGIKAFNVK"
BASE COUNT          527 a          443 c          322 g          860 t
ORIGIN      
        1 aaataaaaga atccggcatt acatttttgt gtttgccgga tttctttata tccaattttc
       61 atctatttct tttttcaact ggcaaaacat atcataaaat ttttcttttg cctgtgaaat
      121 aattgataat gcgatattct gattataaga atgtgttaca tttctatcca aataagtgct
      181 ttctattata acatactctc ttatattttc ttataaaaaa tcaaagaaaa aagcctctgg
      241 atactaaatc cagagactta aaagtgctat tatttacgtt ttttataatt gcgttcttct
      301 tattttacgt tgaaggcttt aattccagga tatacggcac tgtctcctaa agcttcttcg
      361 attcttaata actggttgta tttcgcaaca cgttctgatc ttgaaggtgc tcctgtcttg
      421 atctggcatg tatttaatgc gacggctaag tctgcgatcg ttgtatctgc tgtttctcca
      481 gaacgatgag atgttacagc tgtgtatcct gctttgtgtg ccatcttgat cgcttctaat
      541 gtttcagaga tagatccaat ctggtttaat ttaatcagga tagagtttcc tgctcccatt
      601 tcgatacctt tagaaagtct ttctgtattt gttacaaata agtcatctcc tactaactgt
      661 actttatctc caagtctttc tgtcagtttc ttccatcctt cccagtcttc ttcatctaat
      721 ccatcttcga tcgagatgat tggatatttc tcacataatg cagcccagtg ctcgatcagt
      781 tcttctgctg tatattctgt tcctgctttt ggcagtttgt aatatccttt acctttttca
      841 ctcttccatt ctgaagatgc agcgtccata gcgatcttga agtcttttcc tggttcgtat
      901 cctgcttttt tcactgcttc taagattgtt tcgatggctt cttcatctga ttttaatgct
      961 ggcgcaaatc caccttcatc acctacggat gtagcaagtc ctctttcttt taagattgct
     1021 gctaatgcat ggaatacttc agcacaccat cttaaacatt ctttgaatga tggagctcca
     1081 actggcataa tcatgaattc ctgtacatca agtcctgatg ataatgcatg acatccacca
     1141 tttacgatat tcatcattgg tactggaagt ctgtttcctg atactccacc taagaatctg
     1201 tatagaggta cttcaagaga tgtagctgct gctcttgctg ctgcgatcga tacagctaag
     1261 attgcatttg ctccaaagtt tgatttatca gctgttccgt ctgcatcaat cattgctttg
     1321 tctactgcat atgtatcaga agcatctaat ccgatgattg cttcactgat ctttgtattg
     1381 atattttcta cggcttttgt aacacctttt cctaaatatc tttctttatc tccgtctctt
     1441 aattctagtg cttcaaattc tcctgtagat gcaccacttg gagctgttcc tcttgcgact
     1501 gttccatcaa ctaatgtgat ctccgcttct accgttgggt ttcctctgga atctaagatt
     1561 tctcttccga ctactttttc aatttctaaa taattcattt cgattgtgac tcctttccct
     1621 atacatagca gtactcctcg ctggaagaag ggtaagcttc cagtttgaga tcgttttgtt
     1681 gcaggtgtcg atctcaatcg aggagtccac atgattcatt cacgttttta gttagtgttg
     1741 tctaactgtt atttatgata ctagatttgg atggaaaaat caatggggat atatgataat
     1801 aattatcaaa tatcccctag ttgattattt tcttaatttt ttgatcaact cttttttcgt
     1861 tgtattatta tatgcccata cacgtacatc atttttcgtt gcacttctat attgtttttc
     1921 tgaaatatat gtcaactttc ctttttcctt tttgctatat atttctttat caatgactct
     1981 atgtactgtt ttttctgatt tttctaaatt tgttgtaact ttatttccat cctcatcttc
     2041 gaaatgtcct tcacgttcaa agaaatatga tgcttttttt acaattttct ttccattata
     2101 cttataaata taatattttt cccctttcag gaccattttc acttaatact ga
//
LOCUS       DAOMUE010000321         2133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_14688_length_2133_cov_2.030318, whole genome shotgun sequence.
ACCESSION   DAOMUE010000321 DAOMUE010000000
VERSION     DAOMUE010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14688_length_2133_cov_2.030318"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLC71_10550"
     CDS             <1..229
                     /locus_tag="PLC71_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03116.1"
                     /translation="GEVRIPNKELMNKFDDMIHKVSKLKAQKYNKKKWRYVQKQLKKS
                     KKILYSNAATQKSINSAKSDLKKSINKLRRK"
     gene            232..1494
                     /locus_tag="PLC71_10555"
     CDS             232..1494
                     /locus_tag="PLC71_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03117.1"
                     /translation="MKKRKLVFAITAITVFSAMMLTSNTKAQAAAKKTYTITPKSSPY
                     KGKYKKAKGYYNSTTKQYFAIRSYLELLEKKGGGKLVIKKGTYKIPNVLYIPSNVTIE
                     LKDGVTIKKIMKTKAKKMKPGGGIFELLEPSKAKKKGVHGRYNGVHDVKIYSTGKAVI
                     DQDYQGKTGQNCIALVMCHNRNVTIEGITFKNMKYGHFIEMDASQNVNVNRCTFTGYK
                     ASKRHTSEAINLDTPDKKTRGFTHGWSQYDCTPNQNVQITNCIFSNLEKAIGTHQYSV
                     EKYHTDISISDCMIKNCVSGGIEMMNWQRVSLTNTRFMNIGKNSKGKYTSYNRDRKIR
                     AILVRGGVSEINIKDCTFQNLPRVMQCMPWKNQNTATQYPMIYNHITQEEYQRIASQN
                     KVLRGVDVPYIIVNTRYNDYNYPEKYYF"
     gene            1559..>2133
                     /locus_tag="PLC71_10560"
     CDS             1559..>2133
                     /locus_tag="PLC71_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRM03118.1"
                     /translation="MKQYDYLIVGSGLYGAVFAQQATAKGKKVLVIDKRPNIAGNVYT
                     EDIEKIHVHKYGAHIFHTNNKKVWNYITKFAEFNRFTNSPVANYKGELYSLPFNMYTF
                     NKMWGVITPQEAADKIEQQKKEAGITEPKNLEEQAISLVGTDIYEKLIKGYTEKQWGR
                     PCTELPSFIIKRLPVRLTFDNNYFNALYQGIP"
BASE COUNT          860 a          327 c          425 g          521 t
ORIGIN      
        1 tggggaagta agaattccga ataaagagct gatgaataaa tttgatgata tgattcacaa
       61 agtatccaag ttaaaagcac aaaagtataa caagaagaag tggaggtatg tgcagaagca
      121 attaaagaag tcaaagaaaa tattatacag caatgcggca acacagaaat caattaatag
      181 tgcaaaatcg gatttgaaaa agagtataaa taaactaagg agaaaataaa tttgaaaaaa
      241 agaaaattag tgtttgcaat tacagccatc acggtatttt cagcgatgat gttaacatca
      301 aacacaaaag cgcaagctgc agcaaagaag acatacacga tcacgccaaa gtcatcacca
      361 tataaaggaa agtacaaaaa agcaaaagga tactacaatt ccacaacaaa acagtatttt
      421 gcgatccgct cttatttaga actgctcgaa aaaaagggtg gaggaaaatt agtcataaaa
      481 aaggggactt ataagatacc aaatgtttta tatattccat ccaatgttac gatcgaactc
      541 aaagatggtg taacgattaa aaagatcatg aaaaccaaag caaagaaaat gaaaccaggt
      601 ggtggtattt ttgaattgtt ggaaccatcg aaagcgaaga aaaagggtgt gcatggacga
      661 tataatgggg ttcatgatgt taagatatat tcaacaggaa aggcagtgat cgatcaggat
      721 tatcagggaa aaactggtca gaattgtatt gcacttgtta tgtgtcataa cagaaatgtt
      781 acgattgagg gaatcacatt taaaaatatg aaatatggac attttatcga gatggatgca
      841 tcacagaatg taaatgtcaa tcgttgtacc tttacaggat ataaagcaag taaacgtcat
      901 acatccgagg caatcaatct agatactcca gataagaaaa caagagggtt tactcatggc
      961 tggagtcagt atgactgtac gccaaatcag aatgtgcaga ttacaaactg tatattttca
     1021 aatctcgaaa aagcaatagg aacacatcag tattctgtag aaaaatatca cacagatatc
     1081 tcaatctctg attgcatgat aaaaaactgt gtcagcggtg ggattgagat gatgaactgg
     1141 caaagagtgt ctttgaccaa tacgagattt atgaatattg gaaaaaacag taaaggaaaa
     1201 tatacttcct ataacaggga tcgtaagatc agagcaatct tggtgcgagg aggtgtttca
     1261 gagattaata tcaaggattg tacgtttcag aatcttccaa gagttatgca gtgtatgcca
     1321 tggaagaacc agaatacggc aacgcaatat ccaatgatat ataatcatat tacacaagaa
     1381 gaataccaaa ggattgcatc gcagaataaa gtgcttcgtg gtgttgatgt accatatatt
     1441 attgtgaaca cacgttacaa cgattataat tatccagaga aatattattt ttaaaatgga
     1501 tattgggaga taccttgata atatagataa gaagtttaca agaaaggaca aaacaaaaat
     1561 gaaacaatat gattacttga tcgtaggttc cggtttatac ggagccgtct ttgcacagca
     1621 agcaacagca aaaggaaaga aggtcttagt catcgacaaa cgaccaaata ttgcaggaaa
     1681 tgtatacaca gaagatattg agaagatcca tgtacataaa tatggagccc atatcttcca
     1741 tacaaacaat aaaaaagtat ggaattacat tacaaagttt gcagagttta atcgattcac
     1801 aaactcacca gttgcaaatt acaaaggaga attatattct ctgccattta atatgtacac
     1861 attcaataag atgtggggtg tgatcacacc gcaggaagct gcagataaga ttgaacagca
     1921 gaagaaagaa gccgggatca cagaaccaaa aaatttagaa gaacaagcaa tcagcctggt
     1981 cgggacagat atttacgaga aactgatcaa aggatacaca gaaaaacagt ggggaagacc
     2041 atgcacagaa cttccatcct ttatcattaa gagattacca gtacgattaa catttgataa
     2101 taactacttc aatgcactat atcagggaat ccc
//
LOCUS       DAOMUE010000322         2129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_14725_length_2129_cov_2.982642, whole genome shotgun sequence.
ACCESSION   DAOMUE010000322 DAOMUE010000000
VERSION     DAOMUE010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14725_length_2129_cov_2.982642"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(480..>1913)
                     /locus_tag="PLC71_10565"
     CDS             complement(480..>1913)
                     /locus_tag="PLC71_10565"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRM03119.1"
                     /translation="SETEYTFLRTLIENDLIPDDVTIQVLTQAREHIIKKTFEALKGA
                     KRAIVHVYNSTSVAQREQVFKKSKEEIMKIATDGAKLLKRLADETDGNFLFEYSPESF
                     TGTEIDYALDVCNAVLDVWQPRPDWKVIINLPVTVQLSMPHVYASQIEYMSKHMKYRE
                     NVVLSLHPHNDRGCGVADTELALLAGADRVEGTLFGNGERTGNVDIITLALNMYTHGV
                     DPKLDFSNLPELTKVYERLTRMSVYERQPYTGQLVFAAFSGSHQDAIAKGMHWRDEKH
                     PEHWNIPYLPIDPHDVGREYESDVIRINSQSGKGGISYVLEENFGFHIPGKMREDVGY
                     TVKDVSDKRHQELVPRDILKVFKEVYVNIDDPCKLKEVHFVQKDGGIEAEISLEKSGV
                     PKLYHGKGNGRLDAVSNALQRHSDMHYSIVTYQEHALNVGSNSQACAYVGVQEPNGNV
                     TWGSGIHEDIITASVLALISAVNRLDQ"
     assembly_gap    1914..1923
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1924..2125)
                     /locus_tag="PLC71_10570"
                     /pseudo
     CDS             complement(1924..2125)
                     /locus_tag="PLC71_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009264481.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
BASE COUNT          610 a          416 c          399 g          694 t
ORIGIN      
        1 cgtttattta ttttttatca tataccagaa gtctaaagtg tggacaatta caagaattga
       61 aaaatttaag tggtgtgtat gttaactgtt atcgagcttc aatttatata gtggcagaca
      121 gatgtttatt atacataccc atacagtaaa acatacacac tgtatggtgg tttgaatagt
      181 gttttgttat atgagtcaga aagaagataa gaagaaatat ttataagaat aaagggcgac
      241 aacatgagca ctttttgaat gcgtttttta caaattcgca gcgccctgtg gttgattgtc
      301 tgaccgtagc tgcgggcatt ttggctcgca gccaggaagt tatcaaagga acaggcgcgg
      361 atcagcgatt tgtaaaaaac gctttcaaaa atgcggagtg tcacccttta ttcttataaa
      421 tatttcttct tatcttcttt ctgactcata taggaccaac actacctaca aatcacaatc
      481 tactgatcta atcggtttac agcgctgatc agtgctaata cagaagcagt aatgatgtct
      541 tcgtggattc cggatcccca agtaacatta ccatttggtt cttgaactcc aacataagca
      601 caagcctgag agttagaacc aacatttaaa gcgtgttcct gataagtaac gatagagtaa
      661 tgcatatcac tgtgtctctg taaagcgtta cttacagcat ccagacgacc atttcctttt
      721 ccgtggtaaa gttttggaac accagatttc tctaaagaga tttctgcttc gatgccaccg
      781 tctttctgta cgaagtgcac ttcttttaat ttgcatggat catcaatatt tacgtaaact
      841 tctttgaata cttttaagat atctcttgga actaattcct gatggcgttt atcagataca
      901 tctttgactg tgtatcctac atcttcacgc atttttccag gaatatggaa tccaaagttt
      961 tcttctagta catagctgat accgccttta ccagactgac tgttgatacg gataacatca
     1021 ctttcgtatt cacgtccaac atcgtgtgga tcgattggaa gatatgggat attccagtgc
     1081 tctggatgtt tttcgtctct ccagtgcata cctttggcaa tcgcatcctg atgagatcca
     1141 gagaatgcag caaataccaa ctgtcctgtg tatggctgac gttcgtaaac ggacattctt
     1201 gttaatcttt catagacttt tgtaagttct ggtaagttag agaagtccag ttttggatca
     1261 acaccgtgtg tatacatatt taatgcaaga gtgataatgt caacgttacc tgtacgttca
     1321 ccatttccaa ataaagtacc ctcgacacga tcagcacctg caagcaaggc aagttctgta
     1381 tcagcaacac cgcatccacg gtcattatgt ggatgaagag ataaaactac gttttcacgg
     1441 tatttcatat gtttgctcat atattcgatc tggctggcat atacgtgagg cattgaaagc
     1501 tgtacagtta caggaagatt gatgatgacc ttccagtctg gacgaggctg ccatacgtca
     1561 agaacggcat tacatacatc taatgcatag tcgatctctg taccagtaaa actctctgga
     1621 gagtattcaa ataagaagtt accgtctgtc tcatcagcca ggcgttttaa taattttgca
     1681 ccgtctgttg caattttcat gatctcttct ttggattttt taaatacctg ttcacgctgt
     1741 gcaacagaag tagagttata tacatgaacg atcgctcttt ttgcgccttt taaagcttcg
     1801 aaagtctttt taatgatatg ttctcttgcc tgtgttaaaa cctgaatggt aacatcatca
     1861 gggatcagat cattttcgat caaagtacgt aaaaatgtgt attctgtttc tgannnnnnn
     1921 nnnctttgaa accaaccttt actaaaagtt taaaaaattc aactttttca tcaagactca
     1981 tcggaatgat cagagcttga tttccatcac gaaggtcaac actacaccat gtaggtgctt
     2041 ttgtgatata ttctttcttt gtccagtcaa gggacgtttc aggtggcata aaatattgtc
     2101 tttcatattt gcttgggttc atcattgct
//
LOCUS       DAOMUE010000323         2127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_14742_length_2127_cov_2.183880, whole genome shotgun sequence.
ACCESSION   DAOMUE010000323 DAOMUE010000000
VERSION     DAOMUE010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14742_length_2127_cov_2.183880"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    457..479
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<480..734)
                     /locus_tag="PLC71_10575"
     CDS             complement(<480..734)
                     /locus_tag="PLC71_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRM03120.1"
                     /translation="MAYTEIVITRFGDHLVSMLLKDGHPVEFQCVSEKNKVDIGNIYI
                     GVVKNVVKNINGAFVEFDQDEIGFLSMRHRQYKAGDEVLVQ"
     gene            complement(734..1874)
                     /locus_tag="PLC71_10580"
                     /pseudo
     CDS             complement(734..1874)
                     /locus_tag="PLC71_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trans-2-enoyl-CoA reductase family protein"
BASE COUNT          589 a          477 c          286 g          752 t
ORIGIN      
        1 aatcagaata aacttacttt ttatcaaaat tacaaaacgg taatcagaca acattaacta
       61 tactattgaa tagaatcccg gtcaaatatt tactttcaaa tatttgcaca aaatagtaaa
      121 atatgaagtt tcttattttt taaggagttt tttattatga acaaatattt ttttaaccac
      181 gacctttctc cgctgcttgg tcttgctgac agtcaaatta tcacttttat cgggggtggg
      241 ggaaaaactt ctcttatgaa tactcttgga aaggaatttg caagccacgg atatccaaca
      301 cttctcacaa ctacaacgca tattatgaag ccagattttc tttctgatga atcttattgg
      361 aaattcctac agacagctta tctgaagtca gcacaagata ttcccctgtt aattctatct
      421 gatctgatag catcggacgt ttttcttctg tcgcttnnnn nnnnnnnnnn nnnnnnnnnc
      481 tgtaccaaca cttcatcacc cgctttatat tgcctatgtc tcatggaaag gaagcctatt
      541 tcgtcttgat cgaactcaac aaaagcccca ttgatattct tgacaacatt ttttacgact
      601 cctatgtaaa tattcccaat atcaacttta ttcttctcac ttacacattg aaattctacc
      661 ggatgaccat cttttaataa catactgacc agatgatcac caaaccttgt gatcacaatc
      721 tctgtatatg ccattattct gtgatactag gaatttctac aacaggatct gtttctgctt
      781 cgtagtcgat tccttcctct ttaaatccaa acatacggta aaaatcttcc caataaccat
      841 caacatctgc aagttctgaa acattatctg tattgatctc ttcccaacgt ttcttcactt
      901 catcctgaat atcatcacga agttccagat catccatacg aacccatccg tgttcatccg
      961 tgatcgtatc ggctaataat ctcttttctt taaacagacg ttcaatctgt tcaatacatc
     1021 cttcatggac attattttcc ttcatgactt tatataatac agacatatac aatggaacaa
     1081 taggaatcgc agcacttgcc tgtgttacca atcctttgtt aacagagatc agcgctttta
     1141 ctccaacttc tttcgtaatc tcatctgctg tctgatgcag atgtttcttt gccgcaccga
     1201 tcgttccttc aaaatagatt ggatacgtaa gttcagatcc gatataagaa tatgcgacag
     1261 tacttgcatc ttctgataac acatctgcat cttttaatgc ctgcatccat aacttccagt
     1321 cttctccacc cataactttg actgtagcct ctacttcctc ttctgtagct gcaggaatgc
     1381 tcttcatacc aatactgtta tccttaaggt tcagattctt atttgtaaac tcctcccctg
     1441 ttgtctttaa tacagaacga tatgtcactc catctggtgt tgttcttcgt ggtgctgcaa
     1501 ggctgtatac aaccagatcc actttcccaa gatctttctt gatcgtttcg atcgtaagat
     1561 ttttgatttc ttttgagaac gcgtctccat tgatcgtctt ggcataaact ccttctccgt
     1621 ttgcaaactg ttcaaatgaa gctgtattat acaaacatga ttccaagggt atctgcatga
     1681 catccaaaag ctgccacgat tcttgatgcc agtccatatc ctgtagaaca tccgatcact
     1741 aacactttct tcgcatctcc aggaatctct ccttgagctt ttacataggc gatctgatct
     1801 tccacattct ttttacatcc ttctggatgt gctgttaagc ataaataatc tttgactctc
     1861 ggtttaatga tcataaattt ttctccttta aaacatctgc atcctataca ggtgtattgt
     1921 ttattatcaa cttattcctt caaattataa tatgtatcgg atttatctgt caaattttta
     1981 taatacactt tttcaaattt tgttggttat cttgggaagt tttcaaaact ttattctcag
     2041 catcaaaaga agaaggtatt tctttttcta aaaaatacct tcttcttatc ctctttattt
     2101 aacgattctt cgtactgtta cgattct
//
LOCUS       DAOMUE010000324         2126 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_14753_length_2126_cov_2.050700, whole genome shotgun sequence.
ACCESSION   DAOMUE010000324 DAOMUE010000000
VERSION     DAOMUE010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14753_length_2126_cov_2.050700"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLC71_10585"
     CDS             complement(<1..1092)
                     /locus_tag="PLC71_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HRM03121.1"
                     /translation="MKTQQEYIEQGEQYILKTYNRFPVVLEKGKGVYLYDADQKKYLD
                     FGAGIAVFALGYGNEAYNQALKDQIDKLIHTSNLYYNIPAVEAAKKIVDASGLKKVFF
                     TNSGTEAIEGALKVARKYAYNKDDSKDYEFIAMNHSFHGRSQGALSVTGNSHYQDGFV
                     PKEFRAVFAEFNNLEDVVSKITDKTCGIICEVVQGEGGIFPADKEFLEGLRKVCDEKD
                     ILLIFDEIQCGMGRCGSLFAHDLYGVKPDVLTLAKALGCGVPVGAFVVGEKADGALVP
                     GDHGTTYGGNPFAAAAINAVFDQFEKLQVPEHAKEIGAYLWDKLEELKERYDCITGHR
                     GIALMQGLEFDADHPVGDIVKKALEKGLII"
     gene            complement(1094..2035)
                     /gene="argB"
                     /locus_tag="PLC71_10590"
     CDS             complement(1094..2035)
                     /gene="argB"
                     /locus_tag="PLC71_10590"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRM03122.1"
                     /translation="MEQMRNEINELIEQSKQKAKVLTEALPYIRRFNNCYVVVKYGGS
                     AMKDKNLQKSVIRDVALLKLVGMKPIIVHGGGKEISKWVRMSGKEPEFYHGLRVTDKE
                     TMEVAEMVLFKVNQELVGMMAEVGVKAVGLSGKDAEMIRVKKKEMPGKDLGYVGEVKS
                     VDTTLLEALIEDDFVPVISPIGLGDNYEPYNINADDTACAVAEALNAEKLVFLTDIEG
                     VFVDPEDKSTLISEMDLKQAKEFIDNGVVGGGMLPKLKNCIEAIEGGVSRVHILDGRL
                     TNCLLLEFFTEKGIGTAILKEPLFDKPLFNEEDEEGC"
BASE COUNT          572 a          503 c          294 g          757 t
ORIGIN      
        1 tataatcaat cctttctcca gtgctttctt aacgatatct ccaactggat gatcagcatc
       61 aaattcaagt ccttgcatta aggcaattcc acgatgtcct gtgatacagt catatctctc
      121 ttttaactct tctaatttat cccacaggta agctccgatt tcttttgcat gttctggaac
      181 ctgtaacttc tcaaactggt caaatactgc attgatcgcc gctgctgcga atggatttcc
      241 accataagtt gttccatgat cacctggtac taaagcccca tcagcctttt cacctacaac
      301 aaacgcccca acaggaacac cacatccaag cgcttttgca agtgttaaga catccggttt
      361 tactccataa agatcatgtg caaacaaaga tccacagcgc cccattccac actgaatctc
      421 atcaaagatc agtaagatat ctttctcatc gcagacttta cgaagtccct ctaagaattc
      481 tttatccgct gggaaaattc caccttcacc ctgaacaact tcacatatga ttccacaagt
      541 tttatctgtg atctttgata caacatcttc taagttattg aattctgcaa atacagcacg
      601 aaattctttt ggaacaaatc catcctgata atgactgtta ccagtcactg ataaagctcc
      661 ctggcttctt ccgtggaatg aatgattcat tgcgataaat tcataatctt tggaatcgtc
      721 tttattatat gcatattttc ttgcaacttt taacgcacct tcgattgctt ctgttccact
      781 gtttgtaaag aacacttttt taagccctga agcatcgaca atcttctttg cagcttctac
      841 tgctggaata ttgtaatata agtttgatgt atggatcaat ttatcaatct ggtcttttaa
      901 tgcctgattg tatgcttcat ttccatatcc taatgcaaat acagcaattc ctgcaccaaa
      961 atccagatat ttcttctggt cagcatcata taaatagaca cctttccctt tttctaatac
     1021 aactggaaaa cgattgtatg tctttaagat atactgttct ccctgctcaa tatattcctg
     1081 ctgcgttttc atcttaacat ccttcctcat cttcttcgtt gaataatggt ttatcaaata
     1141 atggttcttt taagatggct gttccaatac ctttttctgt gaagaattcc agtaacagac
     1201 aatttgttaa acgtccgtct aagatatgga ctctggatac tccaccttcg atcgcttcaa
     1261 tacagttctt aagttttgga agcattcctc ctccaacaac tccgttatcg ataaattctt
     1321 ttgcctgttt aagatccatc tcagagatca atgttgattt atcttctgga tctacgaata
     1381 ccccttcgat atcagttaag aataccagtt tttctgcatt caatgcttct gcaactgcac
     1441 atgctgtgtc atccgcatta atattatatg gttcatagtt atctcctaaa ccaattggag
     1501 aaataactgg tacaaaatca tcttcaatca atgcttcaag aagtgttgta tcgacactct
     1561 taacctcacc gacatatcca agatcttttc caggcatttc ttttttcttt acacggatca
     1621 tttcagcatc ttttccagaa agacctacgg ctttaacccc aacttctgcc atcatgccta
     1681 ccagttcctg attgacttta aataagacca tttcagcaac ttccattgtc tctttatctg
     1741 tcacacgaag tccatggtaa aattctggtt cttttcctga catacgaacc catttactga
     1801 tctcttttcc accaccatgt acgatgattg gtttcatacc tactagtttt aatagtgcaa
     1861 catctctgat cacgcttttt tgaagattct tgtctttcat agcgcttcca ccgtatttta
     1921 caacaacgta acagttattg aaacgacgga tatatggtaa tgcctctgtt aatacttttg
     1981 ctttttgttt tgactgctcg atcaattcgt taatttcatt tctcatctgt tccattgatt
     2041 cttcctttca tagttgtctg cttttatgcc ttttattttg tcgtctatct ataataatct
     2101 tattcttatg atcggtaatc tgcatt
//
LOCUS       DAOMUE010000325         2096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_15032_length_2096_cov_2.317981, whole genome shotgun sequence.
ACCESSION   DAOMUE010000325 DAOMUE010000000
VERSION     DAOMUE010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15032_length_2096_cov_2.317981"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PLC71_10595"
     CDS             complement(<1..836)
                     /locus_tag="PLC71_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HRM03123.1"
                     /translation="MDLQKDIKKLVTYGLDKKLIMPEDEIYTINQYLEVFRLDEYEDP
                     DIEGEEIILPEILDRLTDTAYDRYIIKSDDIVTRDLFDTKLMGILTPKPSQVIKEFRT
                     YYEESPKKATEFFYEFSQDTNYIRRDRVKKDMKWKVNSPYGDIDITINLSKPEKDPKA
                     IAAAKNAKQSSYPKCQLCMENEGYAGRMNHPARQNHRIIPLTINDRKWGFQYSPYVYY
                     NEHCIVFNGQHVPMKIDRAAFTKLFDFVKQFPHYFLGSNADLPIVGGSILTHDHFQGG
                     HY"
     gene            complement(838..1857)
                     /locus_tag="PLC71_10600"
     CDS             complement(838..1857)
                     /locus_tag="PLC71_10600"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRM03124.1"
                     /translation="MMKVRMIEQRDNGVCLFELKNDEIKVITSNLGCHVLSVFTKDRE
                     GNFGDVVLGFENVEDCWHGDGSYMGAIAGRVANRIGDAKFELNGKTYELAANNGKNSL
                     HGGIKGFNQKIFKYELLEDGIRFIYLSPDMEEGYPGNLYLKVVYRLCDNTLKMEYEAV
                     SDQDTLINITNHSYFNLSAGKDKIYHHQLKVKADEIACVDENCLANGTFLKIENTPFD
                     FKEFHEIGERINNDHEQLKLAGGYDHSFMVKDEDDQLVLYDKETGRKMTMTTTLPCIQ
                     VYTGNFLSGGCNGKGGKPYENRDGVALEAQFLPNSIHIEKEPKVILRKGEEYEAVTTY
                     RFEVE"
     gene            complement(1859..>2096)
                     /locus_tag="PLC71_10605"
     CDS             complement(1859..>2096)
                     /locus_tag="PLC71_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRM03125.1"
                     /translation="YSSHDLTNQSVSIGLAISDISLGDKGVSRVHGGGFAGTIQAFVP
                     NDIVSMYKKNMEHVFGEGACHVLKVRPYGGMKVL"
BASE COUNT          555 a          448 c          322 g          771 t
ORIGIN      
        1 tcataatgac cgccttggaa atgatcgtgt gttaagatag aacctccaac gatcggaaga
       61 tcggcattcg atccaaggaa ataatgagga aactgtttta caaaatcaaa tagttttgta
      121 aatgctgccc gatcgatctt cattggtaca tgctgtccat taaatacgat acaatgctca
      181 ttgtagtata catatggaga atactggaat ccccattttc tgtcattgat cgtaagcggt
      241 atgatcctgt gattctgtct tgcagggtga ttcattcttc ctgcatatcc ttcattttcc
      301 atgcaaagct gacactttgg ataagaactt tgctttgcat ttttcgctgc tgcgatcgct
      361 ttcggatctt tctctggttt ggagagattg atcgtgatat caatatctcc atatggacta
      421 tttactttcc atttcatatc ttttttgacg cggtctcttc ggatatagtt ggtatcctgg
      481 ctgaattcat agaaaaactc tgttgctttc tttggactct cttcataata ggtacggaat
      541 tctttgatga cctggcttgg ttttggtgtc aggattccca ttaattttgt gtcaaataaa
      601 tctcttgtca cgatatcatc acttttgatg atatagcggt cataagctgt atctgtcaga
      661 cgatctaaaa tctctggcag gatgatttct tctccttcaa tatcaggatc ttcatattca
      721 tctaatcgaa acacttccag atattgattg atcgtataga tttcatcttc tggcatgatc
      781 agttttttat caagtccata tgttacaagt tttttgatat ctttttgtaa atccatctta
      841 ttccacctca aatcgatatg ttgttactgc ttcgtattct tcgccttttc ttaaaattac
      901 ctttggttct ttttctatat gtatggagtt tggtaagaac tgggcttcta aggcaacacc
      961 atctctgttt tcgtatggtt tcccaccttt tccattacat ccgccagaca ggaagtttcc
     1021 tgtgtatacc tgaatgcatg gaagtgttgt tgtcatcgtc atttttcttc cagtttcttt
     1081 gtcatataga actaactggt catcttcatc ttttaccatg aaagaatgat cataacctcc
     1141 ggcaagtttt aactgttcat gatcattgtt gatccgctct ccgatctcat gaaattcttt
     1201 aaagtcaaat ggtgtgtttt ctatctttaa aaatgttcca ttggcaagac agttttcatc
     1261 aacacatgca atctcatccg cttttacctt taactgatga tgataaattt tatcttttcc
     1321 agcagataaa ttaaagtaag aatgatttgt gatattgatc agtgtatcct gatcggatac
     1381 tgcttcatat tccattttta atgtattatc gcataaacga tacacaactt tcagataaag
     1441 atttcccgga tatccttctt ccatatctgg tgagagatag ataaaacgga ttccatcttc
     1501 caacaattca tatttaaata tcttttgatt aaatcctttg attcctccat ggagagaatt
     1561 ctttccattg tttgctgcca attcatatgt ctttccattt aattcaaact ttgcatctcc
     1621 gatacgattt gcaacacgtc cggcgatcgc tcccatgtaa cttccatctc catgccaaca
     1681 atcttccaca ttctcaaatc caagaacaac atccccaaag tttccttctc gatcttttgt
     1741 aaatacagat aatacatgac atccaaggtt acttgtgatc actttgatct catcattttt
     1801 taattcgaat aagcagactc cgttatctct ttgttctatc attcttactt tcatcatctt
     1861 ataatacctt cattcctccg tatggacgta cttttaagac atgacatgct ccttcaccaa
     1921 atacatgttc catgttcttt ttgtacatac taacaatatc atttggcaca aacgcctgaa
     1981 tcgttcctgc aaatcctccg ccatgaactc ggctgactcc tttatcacct aaggaaatat
     2041 cactgatcgc caatccgatt gatacactct gatttgtcaa atcatgactt gaatat
//
LOCUS       DAOMUE010000326         2075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_15219_length_2075_cov_2.156436, whole genome shotgun sequence.
ACCESSION   DAOMUE010000326 DAOMUE010000000
VERSION     DAOMUE010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15219_length_2075_cov_2.156436"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLC71_10610"
     CDS             <1..292
                     /locus_tag="PLC71_10610"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRM03126.1"
                     /translation="IPKIAVMPTCRRDVLTLLCATEEMYTEHADRPIITMSMAGTGLI
                     SRLCGEVFGSALTFGAAKKASAPGQAAVNDLNNMLQFLHEEEKILGYSFPLF"
     gene            418..912
                     /locus_tag="PLC71_10615"
     CDS             418..912
                     /locus_tag="PLC71_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HRM03127.1"
                     /translation="MLKWLDENLEEFLMVALLIAMTVIMGIQGFARYALGASLSWSEE
                     LTRYLFIWSGFISVSYCTKKCISIKIEQFVAMFPRRGKALFKVVNHTIELALFLYLIP
                     YAVLYLKSAFESGQVSPACQIPMYYIQAAPLFSFVLVAFRIIQRWIIEFKIVIRKDEE
                     KEEK"
     gene            913..>2075
                     /locus_tag="PLC71_10620"
     CDS             913..>2075
                     /locus_tag="PLC71_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HRM03128.1"
                     /translation="MSAAVVFLLFFICLIIAIPISIALGIVSVLPGAFDPSFTASGTF
                     VIRSMLGGIDSFPLLAVPMFVLSGMIMAKGGISKKIFDVFAFFIGKLTAGMPCAVIVT
                     CLFYGAISGSAPATVAAVGSMTIPILVRLGYDKSFATAIVAVAGGLGVIIPPSIPFIM
                     YGMASGESVSDLFMAGVVPGLLIGALLMVYAIYYCKKHGEDKEKIAAEIDQLHAKGFI
                     GVFKESFFAILSPVIILGCIYTGIASPTEAAVISVFYSLVVSLFIYKSMKFKDIWGIM
                     VEGVRTYAPILFILAASIAFSRVLTLMRVPQSISAWILGNFHNKIVILLVINIFLLIV
                     GMVMDTTPAILILTPILLPIVTAIGMNPIHFGVMMVVNLAIGFVTPPIGVNLFV"
BASE COUNT          596 a          340 c          446 g          693 t
ORIGIN      
        1 tatccctaag atcgcagtta tgccaacttg cagacgtgac gtattaacac ttctttgtgc
       61 aacagaagaa atgtatacag aacatgcaga tcgtccgatc attacaatgt ctatggcagg
      121 aacaggactg atcagccgtc tttgcggtga agtatttgga tctgctttaa catttggagc
      181 agcgaagaaa gcatctgcac ctggacaggc agcagtcaat gacttaaata atatgcttca
      241 gttccttcat gaagaggaga agattcttgg ttattctttt cctctttttt aaatatataa
      301 aaaatatttg ctgaaatcct attttttcag tataatttag tagggcaaat cgttaaagtt
      361 ttgccatagt ttgtgaaaat aaagactttt gttatagaaa gtagaaagag gtgaatcatg
      421 ctaaaatggc ttgatgagaa tctggaagaa tttctgatgg tagcattatt gatcgccatg
      481 acagtgatca tggggattca gggatttgca agatatgcac ttggagcttc tctttcatgg
      541 tcagaggaac tgacaagata tttgtttatc tggtcaggct ttatcagtgt tagttattgt
      601 actaagaaat gtatttccat taaaattgaa cagtttgtag caatgtttcc aagaagaggg
      661 aaagcattgt tcaaagtggt gaaccacacg atcgaacttg ccttgttttt atatctgatt
      721 ccatatgcag ttctttatct aaagagtgca tttgaaagtg gacaggtaag tcccgcatgt
      781 cagattccaa tgtattatat tcaggcagcc ccgctgttta gttttgtttt agtagcattt
      841 cgtatcatcc agcgttggat cattgaattt aagatcgtga tccgcaaaga tgaagaaaag
      901 gaggaaaaat aaatgtcagc agcagtcgta tttttactct tctttatttg tctgatcatt
      961 gcaattccga tttcgatcgc ccttgggatc gtatctgtac ttccaggagc atttgatcca
     1021 tcatttacag caagcgggac atttgtcatt cgttctatgc ttggaggaat tgatagtttc
     1081 ccattacttg cagttccaat gtttgtatta tctggaatga tcatggcaaa agggggaatc
     1141 tctaagaaga tttttgacgt ttttgcgttt tttataggaa aattaacggc aggaatgcca
     1201 tgtgcagtaa ttgtaacctg cttattttat ggagcaatct caggatctgc acctgcaact
     1261 gtagcagcag taggaagtat gacaattcca atcttagttc gtttaggata tgacaaatca
     1321 tttgcaacgg cgatcgtagc ggtagctggt ggactgggag ttatcatacc gccaagtatc
     1381 ccatttatca tgtatggtat ggcatctggg gaatctgtaa gtgatctgtt tatggcagga
     1441 gttgtaccag gattattgat tggtgcctta ttgatggtgt atgccattta ttattgtaaa
     1501 aaacatggag aagataagga aaagatcgca gcagagatcg atcagttaca tgcaaaagga
     1561 tttattggag tatttaaaga aagctttttt gcaatcttaa gtccggttat catccttgga
     1621 tgtatttaca caggaatcgc atccccaaca gaagcagcgg ttatttctgt attttactct
     1681 ttagtagtca gtttatttat ctataaatcc atgaaattta aagatatctg gggaatcatg
     1741 gtagaaggtg tgagaacata tgcacctatt ctgtttatct tggcagcatc cattgcattc
     1801 tcaagagtat taacattgat gcgtgtacca cagtccatta gtgcatggat cttaggtaac
     1861 ttccataata agattgtgat cttgcttgtg atcaatatat tcttgttgat cgtaggtatg
     1921 gtcatggata cgacaccagc gattcttatt ttgacaccaa ttttattacc gatcgttaca
     1981 gcaatcggta tgaatccgat tcattttggt gttatgatgg ttgtaaacct tgcaattggt
     2041 ttcgttacac caccaattgg agtcaactta tttgt
//
LOCUS       DAOMUE010000327         2052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_15460_length_2052_cov_2.069104, whole genome shotgun sequence.
ACCESSION   DAOMUE010000327 DAOMUE010000000
VERSION     DAOMUE010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15460_length_2052_cov_2.069104"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1275)
                     /gene="sstT"
                     /locus_tag="PLC71_10625"
     CDS             complement(37..1275)
                     /gene="sstT"
                     /locus_tag="PLC71_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HRM03129.1"
                     /translation="MKNLLQKWNSLSLIVRILIGLIIGAILGVAAPKATGLALLGDLF
                     VGALRAVAPILVFVLVIASLANSKSNGAKNMRIVIILYMLTTFIGAVVAVCGSFLFPI
                     TLRLGKAATSEAAPPTGVLEVLKNLLMNIVSNPVDSLVKANYIGILAWAVVFGLALKA
                     ATPGTKKALHDISAAVTKAVHWIINCAPFGILGLVYNTVSTNGLSIFTDYGKLLALLV
                     GCMLFTVFVTNPIIVFSMLRRNPYPLILKCLKESGLTAFFTRSSAANIPMNMALCEKL
                     GLNEDNYSVSIPLGSTINMDGAAITITVMSLAAAHTMGISVDIPTAIILSVLAAVSAC
                     GASGVAGGSLLLIPLACSLFGISNDVAMQVVGVGFIIGVVQDSVETCLNSSSDVLLTA
                     TAELYQRRKAGEDIVLIPNK"
     gene            1571..>2052
                     /locus_tag="PLC71_10630"
     CDS             1571..>2052
                     /locus_tag="PLC71_10630"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004849991.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRM03130.1"
                     /translation="MKQVILTGDRPTGRLHVGHYVGSLKERVRLQNSGKFDEIYIMIA
                     DAQALTDNAEHPEKVRQNIMQVALDYLACGLDPEKVNIFIQSMVPELTELSFYYMNLV
                     TVARVQRNPTVKAEIKQKNFEASIPVGFFTYPISQAADITAFRATVVPAGEDQMPMVE
                     "
BASE COUNT          720 a          373 c          384 g          575 t
ORIGIN      
        1 tatatatttc cacaaatttt atttattcat aagtttttat ttattaggga tcaatacaat
       61 atcttcccca gcttttcttc tctgatataa ctcagcagta gctgtaagta atacatcaga
      121 agaagagttt aagcaagttt caacagaatc ctgtactact ccgatgatga atccaacacc
      181 aacaacctgc atggcaacat catttgagat accaaataaa gaacatgcaa gagggatcaa
      241 cagcagggat ccgccggcaa caccagaagc accacatgca gaaacagcag ctaagacact
      301 taagatgatt gctgtaggaa tatcaacaga gatacccatt gtatgagcag ctgcaagaga
      361 cataactgtg atcgtgatcg cagcaccatc catgttgatc gtagaaccaa gagggataga
      421 tacagaatag ttatcttcat ttaatcccag tttttcgcaa agagccatgt tcataggaat
      481 gtttgcagca gaacttcttg taaagaatgc tgttaaacca ctttctttta agcattttaa
      541 gatcagtgga tatgggtttc tgcgaagcat actaaatacg atgattgggt ttgttacaaa
      601 tacagtaaat aacatacatc caactaatag ggcaagcagt ttaccataat cagtaaagat
      661 gcttaaaccg tttgtagata cagtgttata tacaagtcca agaataccaa atggagcaca
      721 gttgatgatc cagtgtacag cttttgtaac tgcagcagag atatcatgaa gagctttttt
      781 agttccagga gttgcagctt ttaatgcaag tccaaaaaca acagcccatg caaggatacc
      841 gatgtagttt gcctttacaa gggaatcaac agggtttgat acgatattca ttaataagtt
      901 ctttaatact tctaacacac ccgttggagg agcggcttca gatgtagcgg cttttccaag
      961 acgtaatgtg attgggaaga gaaaacttcc acatactgcg acaacagcac caataaaagt
     1021 tgttaacata taaaggatga taacaatacg catatttttt gcaccgttgc tcttagaatt
     1081 tgctaaagaa gcaatgacaa gtacaaatac aaggattggg gctacagcac gaagagcacc
     1141 aacaaataaa tctccaagta gagcaagccc tgttgctttt ggagcagcta caccaagaat
     1201 agcaccgatg atcagaccga ttaagattct tacgatcaga cttaagctat tccacttttg
     1261 taataagttt ttcattttaa aaattccttc tttcattatt tttttatttt taagtgatat
     1321 tttgatactt acatcatatt ttcttaaaaa taaagaatca atctcatcta ttatatttac
     1381 ttttattcct taagtcaata caaaatgtct tttttacggg aatgaatgtc tttctagtgc
     1441 ggacacacaa aaatcttttc acaatgaaat attgacactt tacgcatttg attttacaat
     1501 aaaacataag agtatagatc ataagagaag atgtacaaag tctatacgga aagcggaaaa
     1561 ggagaaaata atgaaacagg taatattaac aggagacaga ccaacgggac gactgcatgt
     1621 tggacattat gtaggatctt taaaagaacg agtgagactg cagaattctg gtaaattcga
     1681 cgaaatttat atcatgatcg ctgatgccca ggcattaaca gataatgcag aacatccaga
     1741 gaaagtaaga cagaacatta tgcaggtagc acttgattat cttgcatgtg ggttagatcc
     1801 agagaaagtc aatatcttta ttcagtctat ggtaccagaa cttacagaac tgtctttcta
     1861 ctatatgaac ttagttacag ttgcaagagt gcagcgtaac ccaacagtaa aagcagagat
     1921 caaacagaag aattttgaag caagcattcc ggttggattt ttcacatatc cgatcagtca
     1981 ggcagcagat atcactgcat ttagagcaac ggtcgtacca gcaggagaag atcagatgcc
     2041 aatggtagag ca
//
LOCUS       DAOMUE010000328         2040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_15580_length_2040_cov_2.287154, whole genome shotgun sequence.
ACCESSION   DAOMUE010000328 DAOMUE010000000
VERSION     DAOMUE010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15580_length_2040_cov_2.287154"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="PLC71_10635"
     CDS             <1..1247
                     /locus_tag="PLC71_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM03131.1"
                     /translation="RMLAGFETPTSGEILFNGKDISELPPNERKFNTVFQKYALFPHL
                     NIYDNISFGLKEKKVPKNEIKKKVKRVLEIVDLEGFEKRKIDSLSGGQQQRIAIARAI
                     VNEPRILLLDEPLSALDYKMRQEMQIELKNMHKELGITFIFVTHDQEEALMMSDKIVV
                     MSKGQIQQVGTPEEIYNKPQNAFVADFIGESNIFDGMKKEGNLIHFAHHDFVCHDDHE
                     PGTRLDVVIRPEDVVLTAPDKGKFTGEVISCLFCGKFYEVAVLSDKNEIEAHILHELT
                     PGTKVGVDFTADTIHTMPKDLKVNHYIGTIDKDGWLELTDGRIPVKEKEPKAHPEGTR
                     LKMYFPSKLAKLSDDPEEGYFQGNIISIIYKGDHYNYRVLSENKVEYSVDDEDLWNIG
                     DFVSVVVPEDAMVFESVSEDAE"
     gene            1244..2029
                     /locus_tag="PLC71_10640"
     CDS             1244..2029
                     /locus_tag="PLC71_10640"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM03132.1"
                     /translation="MKNDRLKSILGIPYFLFLVIFVVCPVLILLYYAFTNGAGEFSLA
                     NFTLFFTDSKAIGTLIYSVMVAVVTTIVCLLIAYPVAYLLAKGEFRRGNVLLMLFVMP
                     MWINFTLRITALKEILTLIEGNLAFHPFLNTVIGMTYDFLPFMILPIYNTIAKIDHSY
                     IEAAKDLGAKSYRVFLRVILPLSIPGIISGITMVFLPSMTNYVVLDMMYNSTYIMGSL
                     VGSYFSMYDWNNGSVISMILLILILLLSTITGEFSDKEDNAYE"
BASE COUNT          660 a          315 c          436 g          629 t
ORIGIN      
        1 taagaatgtt agcaggcttt gaaacaccaa cgagtggaga aatcttattt aatggaaagg
       61 atatcagtga gcttcctcca aatgagagaa agtttaatac tgtattccaa aaatatgcat
      121 tgtttccaca tttaaatatc tacgataata tttcttttgg actaaaagaa aagaaagtac
      181 cgaagaatga gattaaaaaa aaggtaaagc gtgtgcttga aatcgtagac ttagaaggat
      241 ttgagaaaag aaagattgac agtttatctg gcggacagca gcaacgtatt gcgatcgcac
      301 gtgcgatcgt aaacgagccg aggatcttat tattagatga gccgttaagt gcccttgatt
      361 ataagatgcg tcaggagatg cagatcgagt taaaaaatat gcataaggaa ttaggaatca
      421 cctttatctt tgtcacacat gatcaggaag aagcattgat gatgtctgat aagattgttg
      481 ttatgtcaaa aggccagatt cagcaggttg gaacaccaga agagatttat aataaaccac
      541 agaatgcctt tgttgctgat tttatcgggg aaagtaatat ttttgatggt atgaaaaaag
      601 aaggaaatct gatccatttt gcacatcatg attttgtatg tcatgatgac catgaacctg
      661 gaacaagact agatgttgtc atccgcccag aagatgttgt tttaacagct ccagacaaag
      721 gaaaatttac tggagaagta atctcatgtc tgttctgcgg taaattttat gaagtggctg
      781 ttttatcaga caaaaatgaa attgaagcac atatcttgca cgagttaaca ccaggaacaa
      841 aagttggggt tgattttact gcagatacga tccatacgat gccaaaggat ctcaaagtca
      901 accattatat tggtacaata gacaaagatg gttggttaga acttacagat ggtcgtattc
      961 ctgtgaagga aaaagagcca aaagcacatc ctgaaggtac aagattaaaa atgtattttc
     1021 catcaaaact tgcaaaatta agtgatgatc cggaagaagg atattttcag ggaaatatca
     1081 tctcgatcat ttacaaagga gatcattata attatcgagt tttaagtgag aataaagtag
     1141 aatattcggt tgatgatgaa gatttatgga atatcggtga ctttgtcagt gtggtagttc
     1201 cagaagatgc catggtattt gagagtgttt cggaggatgc agaatgaaaa atgatagact
     1261 aaaaagcatt ctgggcattc cgtattttct atttctagtc atttttgtcg tctgtccagt
     1321 attgatcttg ctgtattatg catttaccaa tggagcgggt gagttttctc ttgcgaattt
     1381 tacattgttt tttacagaca gtaaagcaat cggaactttg atctacagtg tgatggttgc
     1441 tgtcgtgaca acgatcgtct gccttttgat cgcatatcca gttgcgtatc ttcttgcaaa
     1501 gggagaattc cggcgaggga atgttttatt aatgttattt gtaatgccta tgtggatcaa
     1561 cttcactttg cgtatcacgg cattaaaaga aatcttgaca ttgatcgaag gaaatcttgc
     1621 atttcatcca ttcctaaata ccgtgatcgg tatgacttat gattttctcc catttatgat
     1681 cctgccaatc tataatacga tcgcgaagat cgatcatagt tatattgaag cggcaaaaga
     1741 tctgggggca aagtcttatc gcgtattttt acgtgtgatc ctgccattgt ctattcctgg
     1801 aatcatcagt gggatcacga tggtatttct tccatctatg acgaattacg ttgtgcttga
     1861 tatgatgtat aacagcacat atattatggg aagtcttgtt ggaagctatt tcagcatgta
     1921 tgactggaac aatggatctg ttatttccat gatcctcttg atcttaatct tattattaag
     1981 tacgatcaca ggagaatttt cagacaagga ggacaatgca tatgaataaa aaaacaatac
//
LOCUS       DAOMUE010000329         2039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_15582_length_2039_cov_8.654234, whole genome shotgun sequence.
ACCESSION   DAOMUE010000329 DAOMUE010000000
VERSION     DAOMUE010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15582_length_2039_cov_8.654234"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1459)
                     /locus_tag="PLC71_10645"
     CDS             complement(86..1459)
                     /locus_tag="PLC71_10645"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRM03133.1"
                     /translation="MAYRVTQRIISSDDLLYPYFDDLCRKSKLLYNAALFRVRNIFTG
                     YDKEHRTENEVEVFQEVALLQRSYPNMHVRRVISYTHLEKMMRVTENADFFSGLPRQT
                     AQQMVKQSVTDFKNWLASLREYKKHPEKYLGKPKMPRYKKSDLTTVIITNQDAVLYRD
                     DIGMSLKLPLQKQRLYFSNLSSDPVLKEVKIKPYYGRFLLCLTLEEPDVAFDPSGSHV
                     CAIDLGTDNFAAIVCDDHSSAIYKGGAVLSKIQWFHKQRAKYVSIITKGHEKKHAVSK
                     RLRDLSFHYANFVKDQCHKISRSIIDFCMEHQCGTLILGVNLLWKQRSNMNKINNQNF
                     VSMPITLLRTMITYKALNAGIRIIEQEESYTSKADLIANDRIPTYGVDDKDASFSGKR
                     IKRGLYRCSNGMILNADCHAAANIMRKAIPDIWKDTRDYTFLSAPDVYGFHKLNLKGI
                     PVKGIAA"
     gene            1550..1972
                     /gene="tnpA"
                     /locus_tag="PLC71_10650"
     CDS             1550..1972
                     /gene="tnpA"
                     /locus_tag="PLC71_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HRM03134.1"
                     /translation="MRKKDNTDYYINRHSCFLLQYHIVFVTKYRHPVLTGKVKDVVYE
                     RIHYVADQRGFHILEINGESDHVHILMEADTTTSPAELANVLKTQTARKARKLYGETI
                     LKKYYWKPYFWSDSYFISSVSENSLHTIKQYIRNQGKR"
BASE COUNT          599 a          410 c          412 g          618 t
ORIGIN      
        1 ctatggaagt ggcttctaac agtcgtgaca gttacccatt tctgaccaaa agattggctg
       61 tccgtaccaa tcaaggcata ttcacttatg ccgctatccc tttgacggga atacctttca
      121 gattcagttt gtggaatcca tacacatcag gagcagaaag aaacgtataa tctctggtat
      181 ctttccagat atcagggatc gctttccgca tgatatttgc tgctgcgtga caatccgcat
      241 ttaagatcat tccgttagag caacggtata aaccacgttt gatcctcttt ccagaaaacg
      301 aagcatcctt atcatcgaca ccataggttg ggatcctgtc atttgcgatc agatccgctt
      361 tggaagtata actttcttcc tgctctatga ttctgatgcc ggcattcaga gctttatatg
      421 tgatcattgt ccgcaggaga gtgatcggca tggagacaaa attctggttg ttgatcttat
      481 tcatattgga tctctgcttc cagagcagat tcacaccaag gatcagtgtt ccacactggt
      541 gttccataca gaagtcaatg atactacgac tgatcttgtg gcactggtct ttcacaaaat
      601 tggcataatg gaaagaaagg tctcttaacc gcttggaaac agcgtgtttc ttttcatgac
      661 cttttgtgat gatggagaca tactttgctc tctgtttatg gaaccattgg atcttggata
      721 agacagctcc gcctttgtat attgcggatg agtgatcatc acatacgatc gctgcgaagt
      781 tatctgttcc aagatcaatg gcacatacat gagatccgga aggatcaaat gcaacatctg
      841 gttcttccag tgtcagacag agtaaaaaac gaccataata cggtttgatc ttgacttctt
      901 ttaatacagg atccgaagaa agatttgaaa agtacaagcg ttgcttttgc aaaggcagtt
      961 ttaaggacat cccgatatcg tcccgataca agacagcatc ctggtttgtg atgatcacag
     1021 tcgtcaggtc acttttctta tagcgaggca tcttaggctt acccagatac ttttctggat
     1081 gctttttata ttctcttaaa gaagcaagcc agtttttgaa atctgtgact gactgtttga
     1141 ccatctgctg tgctgtctgt cttggaagtc cggaaaagaa atctgcattc tctgtgacac
     1201 gcatcatctt ttcaaggtgt gtataagaga tcactctgcg gacgtgcatg tttggataag
     1261 atcgttgtaa taacgcaact tcttgaaaga cttcgacttc gttctctgtc cgatgttctt
     1321 tgtcataacc ggtaaagata ttccgcacac gaaaaagagc tgcattgtac aacagcttgg
     1381 actttctgca cagatcgtca aaatatggat ataacagatc atccgaagaa atgattcttt
     1441 gtgtcacacg gtatgccata tggttgtctc ctttctgata tgttgaaaat atcgtacttt
     1501 tatagtataa ttataccaaa catatgttta gctgtaaagg agatacatca tgcgaaagaa
     1561 agataataca gattattata taaaccggca tagttgcttt ttattgcaat accatatcgt
     1621 gtttgttaca aaatacagac atccagtctt aacaggcaaa gtaaaagatg ttgtttatga
     1681 acgtatccac tatgtagcag atcaaagagg gtttcacatc ctagagatca atggcgaatc
     1741 ggatcatgtc catatcctga tggaagctga tacaacaact tcaccggcgg aacttgcaaa
     1801 tgttttaaaa acacaaacag cacgaaaagc acgaaagttg tatggcgaaa ccattttaaa
     1861 aaaatattac tggaaacctt atttttggtc ggatagttat tttatttcat ctgttagtga
     1921 aaacagtctg cacacaataa aacagtatat tcggaatcag gggaaacgat aatgtgtccg
     1981 tcaggacgca ttcacccacg cccatattaa aatatggacg tggtactctg cttagattt
//
LOCUS       DAOMUE010000330         2033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_15645_length_2033_cov_2.308898, whole genome shotgun sequence.
ACCESSION   DAOMUE010000330 DAOMUE010000000
VERSION     DAOMUE010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15645_length_2033_cov_2.308898"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PLC71_10655"
     CDS             <1..939
                     /locus_tag="PLC71_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRM03135.1"
                     /translation="GYGTDQYCAQAKEKIKQAFQCPDGEVFFLVGGTQTNQVIISTML
                     APYEGVIAAKTGHVSVHEAGAIEYSGHKVIELEEKDGKLAAEDIKKCIEDFYSDDNHE
                     HMVYPGMVYISFPTEYGTLYSKQELTEISNICRNYGIPLFIDGARLGYGIESKENNLR
                     PEEIAELCDVFYIGGTKVGALCGEAVVFPHHNTPKHFVTQIKQRGALLAKGRLLGIQF
                     DTLFTDNLYFEISKHAIEMAEKLKEVFHKKGYTFFLESPTNQQFIILENEKMKELSKN
                     VEFGFWEKYDDNHTVVRFATSWSTDENSIDQLEEYL"
     gene            1118..1873
                     /locus_tag="PLC71_10660"
     CDS             1118..1873
                     /locus_tag="PLC71_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03136.1"
                     /translation="MKKFLSMTVLLLIIVLAGCQKDQTTKSKQKTNTNDAKVVKTEKL
                     ETMSYEDFYENLKEEELNDLDIIQVADQKITAVIGNYFLVLNENKKNHTYKINKIIDL
                     HPYGMNYYYNEESTMFYPSKDGEKYLIYNECRYNSKDQTINAKDDKELKSIVIDLKKD
                     EVTYRKGNHLENLKKKEGVSENNYPKIAKFSKDMKQYAKKKKYELMTNFYLKSGKDRF
                     WVMKPFYEENILEGRIFRYHEGKIQCVFKFREP"
BASE COUNT          826 a          260 c          416 g          531 t
ORIGIN      
        1 ggatatggaa cggaccaata ttgtgcacag gcaaaagaaa agatcaaaca ggcatttcaa
       61 tgtccagatg gagaagtctt tttcttagtc ggaggcacac agacaaatca ggtgatcatc
      121 agcacaatgc ttgctcctta cgaaggcgtg atcgcagcaa aaacaggaca tgtcagtgtt
      181 catgaagcag gagcgattga atatagtgga cataaagtga tcgaattaga agaaaaggac
      241 ggaaaattag cagcagaaga tattaagaaa tgtatcgaag atttttattc agacgataat
      301 cacgaacata tggtatatcc tggaatggtt tatatctcat ttcctacaga atatggaaca
      361 ttgtattcga aacaggaact aacagagatc tcaaatatct gcagaaacta tggaattcca
      421 ttattcattg atggggcgag attaggttac ggaattgaaa gtaaagaaaa taatctgaga
      481 ccagaagaaa ttgcagaact atgtgatgta ttttatattg ggggaaccaa agtaggagca
      541 ttatgtggag aagcagtcgt atttccacat cataatacac caaaacattt tgtgacacag
      601 atcaagcaaa gaggcgcatt attagctaaa ggaagattgc tgggaatcca gtttgatacc
      661 ctgtttacag acaatcttta ttttgaaatc agtaaacatg cgattgagat ggcagagaaa
      721 ttaaaagaag tcttccataa gaaaggatat acattcttct tagagtctcc aacgaatcag
      781 cagtttatca tccttgagaa tgagaagatg aaagaactga gcaagaatgt agaatttggt
      841 ttctgggaga aatacgatga taaccatacg gttgtgagat ttgcaacaag ctggtcaacg
      901 gatgaaaatt cgatcgatca gctggaagaa tatctgtaat aaattttatt atagaagaaa
      961 tgcagttttg tatgtaggaa atgcagaact gcatttttgt tttataggag aattttatga
     1021 acgaaaagta aaaaaagttg atggataaat atagaaatga tataatgaaa atatggtagg
     1081 ttaaaacaat aaaggcagga taggaaagga gttaactatg aagaaatttt tatctatgac
     1141 agtgttactt ctgatcatcg tacttgcagg atgtcagaag gatcagacaa caaagtcaaa
     1201 gcagaagacg aatacaaatg atgcaaaagt tgtaaaaaca gagaaactag agacaatgag
     1261 ctacgaggat ttttacgaga atctaaaaga ggaagaattg aacgacttgg atatcataca
     1321 agttgctgat cagaaaatca cagctgtgat aggtaattac tttcttgtgc tgaatgagaa
     1381 caagaagaat catacatata agatcaataa gatcatagat ttgcatccat atggaatgaa
     1441 ttattattat aatgaagaat caacaatgtt ttatccatcc aaagatggag aaaaatatct
     1501 aatttataat gaatgcagat ataattcaaa agatcaaaca atcaatgcta aagatgataa
     1561 agaacttaag agtatagtca ttgatttaaa gaaagatgag gtaacatacc gaaagggaaa
     1621 tcatctggaa aatttgaaga agaaggaagg cgtatccgaa aataattatc caaagatagc
     1681 aaaattttca aaggatatga agcagtatgc aaaaaagaaa aaatatgagc tgatgacaaa
     1741 cttctattta aaatcaggaa aagacagatt ctgggtaatg aaaccattct atgaagaaaa
     1801 tattttagaa ggcaggattt ttagatatca tgaaggaaaa atacagtgcg tatttaaatt
     1861 tcgggaacca taaatataac gtatctatta taaaatgaaa aatgccatcc atatattgta
     1921 gatttgataa agaaaaacac aatatatgga tggcaaaaat aattgtcgaa aaaacataaa
     1981 aaagctgttg acatactgta tacagctatg gtaagataat caacgtcgct gaa
//
LOCUS       DAOMUE010000331         2027 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_15707_length_2027_cov_3.152130, whole genome shotgun sequence.
ACCESSION   DAOMUE010000331 DAOMUE010000000
VERSION     DAOMUE010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15707_length_2027_cov_3.152130"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2027)
                     /locus_tag="PLC71_10665"
     CDS             complement(<1..>2027)
                     /locus_tag="PLC71_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM03137.1"
                     /translation="EKYGYDLVKRAREQKLDPVIGRDSEIRNVVRILSRKTKNNPVLI
                     GEPGVGKTAVVEGLAQRIVRGDVPDQLKDKTIFSLDMGSLVAGAKYRGEFEERLKAVL
                     EEVKKSDGQIILFIDELHTIVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLDEYRQ
                     YIEKDQALERRFQPVMVDQPTVEDTISILRGIKDRYEVYHGVKITDSSLVAAATLSNR
                     YITDRFLPDKAIDLVDEACAMIKTEMNSLPAELDEVQRKIMQMEIEEAALKKEDDRLS
                     KERLEELQKELAEMREDFKARKARWENEKASVEKVSKLREEIESVNSEIQIAQRNYDL
                     NKAAELQYGRLPELKKQLEEEEERVAKEDRSMVRESVTEDEIAKIISRWTGIPVAKLT
                     ESERNKTLHLDKILHERVVGQDEAVELVTESIIRSKAGIKDPSKPIGSFLFLGPTGVG
                     KTELAKALAESLFDNEQNIVRIDMSEYMEKHSVARLIGAPPGYVGYEEGGQLTEAVRR
                     KPYSVVLFDEIEKAHPDVFNILLQVLDDGRITDSKGKTVDFKNTILIMTSNIGSSYLL
                     EGIDEDGNIKPEAQDMVMNDLKNHFRPEFLNRLDETIMFKPLTKANITNIIDLLVKDL
                     NRRLADKELSVELTSAAKNYVADHGYEPMYGARPLKRYLQKSVETLAARLI"
BASE COUNT          470 a          483 c          363 g          711 t
ORIGIN      
        1 aagatcaatc ttgcagccag tgtctcaaca ctcttctgca gatatctctt caatggtctt
       61 gctccataca ttggttcata accatggtcg gcaacataat tcttggctgc tgatgttaat
      121 tcaacgctta attctttgtc tgctaatctg cggttcagat ctttcacaag taagtcaatg
      181 atatttgtga tatttgcttt tgttaatggc ttgaacatga tcgtttcatc cagacggttt
      241 aagaattctg gtctgaaatg attcttaagg tcattcataa ccatatcctg tgcttctggt
      301 ttgatattgc catcttcatc gattccttct aacaggtagg aagatccgat attggatgtc
      361 atgatcaaaa tcgtgttctt aaagtctacc gtcttacctt tggaatctgt gattcgtcca
      421 tcgtctaata cctgtaataa gatattaaat acatctggat gggctttctc gatctcatca
      481 aataagacta cggagtaagg ttttcttctg actgcttcag tcaactgtcc gccttcttca
      541 tatccaacat atcccggagg cgctccgatc agtcttgcca cggagtgttt ctccatatat
      601 tcactcatat cgattcgcac aatattctgc tcgttatcaa ataagctttc tgccagtgct
      661 ttagcaagtt ctgtcttacc tacacctgtt ggtccaagga ataagaaaga accgattggt
      721 ttgcttgggt ccttgattcc ggctttggaa cggatgatgg attctgtaac aagttccact
      781 gcttcatcct gtccaacaac tctttcatga aggatcttat caagatgtaa ggtcttattt
      841 ctctcacttt cagtaagttt cgctactgga attcctgtcc aacgggaaat gatcttggcg
      901 atctcatctt ctgtgacgga ttctctgacc atagaacggt cttctttcgc tacgcgttct
      961 tcttcctctt ctaactgttt cttcaattct ggcaggcgac catactgtaa ttcggctgct
     1021 ttatttaaat cataatttct ctgggcaatc tggatctcac tgtttacaga ttcgatctct
     1081 tctcttaatt tggagacttt ctcaacggaa gctttctcat tctcccatct tgcctttctt
     1141 gctttgaagt cttctcgcat ctcggcaagt tctttctgta attcttccag acgttctttg
     1201 cttaatctgt catcttcctt cttaagtgcg gcttcctcaa tctccatctg catgatcttt
     1261 ctctgaactt catccagttc tgctggcagg gaattcatct ctgtcttgat catggcacat
     1321 gcttcatcga ctaagtcgat ggctttatct ggcaagaaac gatctgtgat ataacggttt
     1381 gataaagttg ctgcagctac tagggaacta tctgtgatct taacaccgtg atatacttca
     1441 tatctgtctt tgatacctct taagatagaa attgtatctt ctacggttgg ctgatcgacc
     1501 ataactggct ggaaacgacg ttccaatgcc tggtctttct cgatatactg gcgatattca
     1561 tctaaggttg ttgcaccgat gcagtgtaat tcacctctgg caagcattgg ttttaacata
     1621 tttcccgcat ccatggctcc gtctgtctta cctgcaccga cgatcgtatg aagttcgtcg
     1681 ataaataaga taatctgtcc atcacttttc ttaacttctt ccagaaccgc ttttaaacgc
     1741 tcttcaaatt caccacggta tttggcacct gctaccagtg atcccatatc cagtgaaaaa
     1801 attgtcttat cttttaactg gtctggcacg tcaccacgaa cgattcgctg tgctaatcct
     1861 tcaacaacgg ctgtcttacc tacacccggt tcaccgatca atactggatt attctttgtc
     1921 tttcttgata agatacgaac aacgttacgg atctcgctgt cacgtccgat gactggatct
     1981 agtttctgtt ctctggctct ctttacaagg tcatatccgt atttttc
//
LOCUS       DAOMUE010000332         2020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_15781_length_2020_cov_2.251908, whole genome shotgun sequence.
ACCESSION   DAOMUE010000332 DAOMUE010000000
VERSION     DAOMUE010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15781_length_2020_cov_2.251908"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..>877)
                     /locus_tag="PLC71_10670"
     CDS             complement(73..>877)
                     /locus_tag="PLC71_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HRM03138.1"
                     /translation="SQGVIPILSGLAWGLGYFGMPHILVRFMAIRDPQEVKKSRNIAM
                     IWVLISLSVAVCIGFTGAALYPNVAELAGNGNQRIFIYMTTHLFKGLIPLFMAGVILS
                     GILAATMSTSDSQLLIASSCVSKNLFQGLFKKEMSEEKVLLVSRITTIVIALIGIFIA
                     MDENSSVFGLVENAWAGFGGAFGPLTLFALFWKRTNLKGAIAGMLSGGIIALVWPVTL
                     GKLGGIFGIYCLLPAFIVSSILIIVVSLCTEEPNDVMVKEFEEAKSLND"
     assembly_gap    878..887
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<888..1594)
                     /locus_tag="PLC71_10675"
     CDS             complement(<888..1594)
                     /locus_tag="PLC71_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HRM03139.1"
                     /translation="MKLPILIAMVLYMAMVIWIGVIYSKKTKTSEDYFLGGRGLGPWV
                     TAMSAEASDMSSWLLMGLPGLAYATGFSQAGWTAIGLILGTYLNWKIVAKRLRHYTEV
                     ADNAITVPDFFSNRFKDDKKILSSISAIMILIFFTVYTASGFAACGTLFNSVFGLNYQ
                     KSMIVCAIVIVLYTSMGGFLAASTTDLIQGLLMSFAIVIVLIVGVVNAGGVANVIAHG
                     QAMEGFFDVMKYHDPATG"
     gene            complement(1713..1898)
                     /locus_tag="PLC71_10680"
     CDS             complement(1713..1898)
                     /locus_tag="PLC71_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRM03140.1"
                     /translation="MKKTLYKSRKDRFLFGVCGGLAEYFEVDPTLVRILTAALCTTGT
                     GLLLYIVAAVVMPERVE"
BASE COUNT          604 a          449 c          306 g          651 t
ORIGIN      
        1 gcgagtgggt tgcaaacata tgatctcttt ctataatatt ccaccaataa ttttcgtcat
       61 tcaaatcaca atttaatcat tgaggctttt tgcttcttca aattctttca ccataacatc
      121 gttaggttct tctgtacata aactaactac gatgatcaga atagaagata caataaatgc
      181 tggtaacagg cagtagattc caaaaattcc acctaatttt cccagagtca ctggccatac
      241 aagtgcaatg atacctccac ttaacattcc agcgattgct ccttttaagt ttgttcgttt
      301 ccagaataat gcaaacagtg ttaatggacc aaaggctcca ccgaatcctg cccatgcatt
      361 ttctactagt ccgaatacag aactgttttc atccattgcg ataaagatac cgatcaatgc
      421 gatcacgatc gttgtgatcc ttgaaactaa cagtaccttt tcttcagaca tttctttctt
      481 aaataatcct tggaataagt tctttgatac gcaggatgat gcaattaaaa gctgcgaatc
      541 agatgtactc attgttgctg caagaattcc tgataaaata actcctgcca taaataatgg
      601 aatcagtcct ttaaataaat gtgttgtcat gtagataaag attctctgat ttccatttcc
      661 agctaattct gctacattgg gataaagtgc tgctcctgta aatccgatac atacggctac
      721 acttaaagaa atcaataccc agatcattgc gatatttctt gatttcttta cttcttgtgg
      781 atcacgaatc gccatgaatc ttacaaggat atgtggcata ccaaagtatc caagtcccca
      841 tgcaagtccg gataagattg ggatcacacc ctgtgatnnn nnnnnnncct gttgctgggt
      901 catggtactt cataacatca aagaatcctt ccattgcctg accgtgtgca ataacattcg
      961 ctactccacc tgcattcaca actccaacga tcagtacgat cacaatggca aatgacatta
     1021 ataatccctg aatcaggtct gttgtacttg ctgctaagaa tccacccatg gatgtatata
     1081 atacaataac gatcgcacag acgatcatac tcttctgata atttaatcca aatacagagt
     1141 taaacaacgt tccacacgca gcaaatcctg atgctgtata tactgtaaag aaaataagga
     1201 tcataattgc ggaaatactg cttaagatct tcttatcatc tttaaaacgg ttactgaaaa
     1261 agtctggtac tgtgatcgca ttatctgcta cttctgtata atgacgtaat ctctttgcca
     1321 cgatcttcca gtttaagtat gtaccaagga tcaatccgat tgctgtccat cctgcctggc
     1381 tgaatcctgt tgcgtatgca agtccaggaa gtcccatcaa cagccagcta ctcatatcag
     1441 atgcctctgc actcattgca gtaacccatg gtccaagtcc tcgtcctcca aggaaatagt
     1501 cttcggatgt ctttgtcttc ttactgtaaa tgacaccaat ccagataacc attgccatat
     1561 agagaaccat tgcgatcaat attggtaatt tcatatgatt tctcctcttc tttatgtgta
     1621 tttatcttca tgatcaaagt cttatttgac ctgaattttg acatgattgt ctattttatc
     1681 acattctttg gattgattca agaaaaagac aattactcga cacgctccgg catgacaaca
     1741 gctgccacaa tatataacaa caatcctgtt cctgttgtgc ataatgctgc tgtaaggatt
     1801 cgtaccaaag ttggatctac ctcaaaatat tctgctaatc ctccacatac accaaataaa
     1861 aaacggtctt ttcttgattt atataatgtt ttcttcattt ccttttcctc catctatctg
     1921 ttactttgtt ttacaatatt gtattatcga aatcaatcgt aagatttcca cttccacaat
     1981 ctatattaat ccatcttaat gcattgatat ttcgtttttt
//
LOCUS       DAOMUE010000333         2014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_15832_length_2014_cov_2.763655, whole genome shotgun sequence.
ACCESSION   DAOMUE010000333 DAOMUE010000000
VERSION     DAOMUE010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15832_length_2014_cov_2.763655"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..>243)
                     /locus_tag="PLC71_10685"
     CDS             complement(2..>243)
                     /locus_tag="PLC71_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRM03141.1"
                     /translation="KLKVERVNVFGKNRNVIKLALKSSQGTRIDGMIFEDEDEFREKM
                     GTSKYITCTYYPVINEYQGYKSLQINIQNYFFVEE"
     assembly_gap    244..253
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<254..1738)
                     /gene="recJ"
                     /locus_tag="PLC71_10690"
     CDS             complement(<254..1738)
                     /gene="recJ"
                     /locus_tag="PLC71_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRM03142.1"
                     /translation="MEQWYLYKKKADFNQIGEKFNIDPVIARLIRNRDVVGDEQIQQY
                     LYGELSDISDPFLMKGVKEGVEILKQKVQEGKTIRIISDYDVDGVVSNYILWKAIHDL
                     GGKIDFQIPDRMKDGYGINENIIEKAVEDQIDTILTCDNGIAAADAVAYGKEHGLTMI
                     ITDHHDVPFDTDEAGVRKEVLPPADVVINPKQEACNYPYPLLCGAGVAFQFMRAFYQA
                     MEEDESQLEELLSMLAIATVCDVVDLTGENRIFVKEGLRRIKDTQNIGLKALLNVHDL
                     MDKQIQSYALGFIVGPCINASGRLESAEKALNLFLEEDEEKAKQTAEELKELNDLRKT
                     MTENGVKEALGQAFEYEAKGDKVLVLYLPECHESIAGIIAGRIKDRLNHPVFVLTDAK
                     EGIKGSGRSIEGYSMFEEMMKVKEVFTKFGGHPMAAGCSLEKEKLQEFRKKINENCTL
                     EKKDFAKKVQIDIDMPVDYITMDLIHQLSVLEPFGKENKKPLFAH"
     gene            complement(1803..>2014)
                     /locus_tag="PLC71_10695"
     CDS             complement(1803..>2014)
                     /locus_tag="PLC71_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03143.1"
                     /translation="SLREFALALMVGVIGGAFSSVFLTGPLWYMMKTRIGSDAIKENQ
                     AASQAQPEKITANPNRKKKKKKKRK"
BASE COUNT          520 a          480 c          282 g          722 t
ORIGIN      
        1 gctattcctc cacaaaaaaa taattttgaa tgttgatctg aagactttta tatccctgat
       61 actcattgat cactggataa tatgtacatg tgatatactt tgatgttccc attttctctc
      121 gaaattcatc ttcatcctca aaaatcatac catcaatcct agttccctga ctactcttta
      181 atgctaattt tatcacattt ctattttttc caaaaacatt cacacgttcc acttttaact
      241 ttcnnnnnnn nnngtgtgca aataatggtt ttttattttc ttttccaaat ggctctaaaa
      301 cagataactg atggatcaga tccatagtaa tataatcaac tggcatatca atatcaatct
      361 gtactttctt tgcaaaatct tttttctcaa gggtacagtt ctcgttgatc tttttgcgga
      421 attcctgtaa tttttctttt tccaaagaac atcctgcggc cattggatgt cccccaaact
      481 ttgtgaacac ctctttgact ttcatcatct cttcaaacat ggaatatcct tcgatggaac
      541 gtccagaacc tttgattcct tcttttgcat ctgttaagac aaatactgga tgatttaagc
      601 ggtccttgat ccttcctgct atgatacctg caatactttc atgacattct ggaagatata
      661 atacaagcac tttatctcct tttgcttcat attcgaacgc ctgtcccagt gcctctttta
      721 caccattttc tgtcatggtt ttccgaagat cattcagttc ttttaattct tctgctgtct
      781 gttttgcctt ctcttcatct tcttccagaa atagattcaa tgctttttcg gcagattcca
      841 aacgtccgct cgcattgata catggtccaa caataaatcc tagtgcataa gactgtatct
      901 gtttatccat cagatcatgc acattcagca atgctttcaa cccgatattt tgtgtatctt
      961 tgattcggcg taatccttct ttgacaaaga ttctgttttc tcctgtcaga tctaccacat
     1021 cacaaacagt cgcgatcgca agcatggata acaactcttc caattggctt tcatcttctt
     1081 ccattgcctg ataaaatgcc ctcataaact gaaaagccac tcctgctcca caaagtaacg
     1141 gatacggata attgcatgct tcttgctttg gattgatcac tacatctgct ggtggaagaa
     1201 cctcttttct cactcctgct tcatcggtat caaatggcac atcatgatga tccgtaatga
     1261 tcattgtcag tccatgttct ttgccgtatg caactgcatc tgctgctgca atcccattat
     1321 cacaggtaag gatcgtatca atctggtctt ccactgcttt ttcaatgata ttttcattaa
     1381 ttccatagcc gtctttcatg cggtctggaa tctgaaaatc aatctttcca ccaagatcat
     1441 gaatcgcttt ccacaaaata taatttgaga caactccatc tacatcataa tctgaaataa
     1501 tacggatcgt tttcccctct tgtacctttt gcttcaaaat ctcaactcct tctttgacgc
     1561 ctttcatcaa aaaaggatct gaaatatctg aaagttctcc atacagatac tgctgtattt
     1621 gttcatcccc aaccacatcc ctgttgcgga tcagccgtgc gatcactgga tctatgttaa
     1681 acttttctcc aatctgatta aaatcagcct ttttcttata cagataccac tgttccatca
     1741 tcttactcca tttttcataa aaaagagcat ccagtattta ctgaatactc tttttgaatg
     1801 ttttattttc ttttcttttt cttctttttc ttgcggtttg gatttgctgt gatcttctct
     1861 ggctgtgctt gactggctgc ctgattttct ttgatcgcat ctgatccaat tcgcgtcttc
     1921 atcatatacc aaagtggtcc ggttaaaaat acagaagaga acgctcctcc gatcacaccc
     1981 accatcaatg ctaatgcaaa ttctcttagg gagg
//
LOCUS       DAOMUE010000334         2014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_15834_length_2014_cov_2.400204, whole genome shotgun sequence.
ACCESSION   DAOMUE010000334 DAOMUE010000000
VERSION     DAOMUE010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15834_length_2014_cov_2.400204"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PLC71_10700"
     CDS             <1..814
                     /locus_tag="PLC71_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM03144.1"
                     /translation="KEVVTQYNPDRIIIEPSGVGKLSDVIKAVQGVAEDVELDLNSFV
                     TVADAKKCKMYMKNFGEFYNNQVEYAGSIILSRTGDVAEDKLNESVALLREHNEKAAI
                     ITTPWDELSGEQILKVMEDGNDMVKELLEEEEICPVCGGHHDHEHHHDHDHEHHHHDH
                     DEECSCGCGHDHDHHHHHHADEVFTSWGKETPKKYNKEELDTILQKLSKDDSYGMILR
                     SKGILQTEDGSWVQFDLVPGEYEIREGSADYTGRICVIGSKLKEDELEGLFF"
     gene            833..1786
                     /locus_tag="PLC71_10705"
     CDS             833..1786
                     /locus_tag="PLC71_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRM03145.1"
                     /translation="MYELPAYLVWGFLESGKSTLIKETLSQDYFNDGEKTMILTFEEG
                     EVEYDKEMLEKTNSFVVNIENMEDFTKEFVRGCQRNYYPDRVMIEYNGMYSIDDLMDV
                     VDETDLELYQVIVTVDASTADLYLKNMKSMFMEMFKMADLVIFNRCDDNTNMGSFRRS
                     IKAVNPRAQVGFERADGKEMAQDELLPYDVNADVIKVEDEDYGIWYIDAMERPEVYEG
                     KTVEMRVQVQRSPKMPPGMIIPGRKAMTCCEDDMTFIGYLCRLNQTKSKTLKRILNNE
                     WVTLTAQIHSEYNEAYDKTMPILTAIRIEKSEPPKEQLVYF"
     gene            1856..>2014
                     /locus_tag="PLC71_10710"
     CDS             1856..>2014
                     /locus_tag="PLC71_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HRM03146.1"
                     /translation="MKKYMEERVIESAIYIIEHNATVRQTAKQFGVSKSTVHKDVTER
                     LETINKTLA"
BASE COUNT          751 a          321 c          454 g          488 t
ORIGIN      
        1 aaaagaagta gttacacagt acaacccaga ccgtatcatc attgaaccat caggagttgg
       61 taaattatca gacgttatca aagctgtaca aggtgttgcg gaagatgttg aactagactt
      121 aaacagcttt gtgacagttg cagatgcaaa gaaatgcaag atgtatatga agaactttgg
      181 tgaattctat aacaatcagg tagaatatgc aggatctatc atcttaagcc gtacaggaga
      241 tgtagcagaa gataaattaa atgaaagtgt agcactgctt cgtgaacata atgaaaaagc
      301 tgcgatcatc acaacaccat gggatgagct ttctggagaa cagatcttaa aagttatgga
      361 agatggaaat gatatggtca aagaattatt agaagaagag gaaatctgcc ctgtgtgcgg
      421 tggacaccat gatcacgaac atcatcacga ccatgatcac gaacatcacc atcacgatca
      481 tgatgaagaa tgcagctgtg gatgtggaca tgaccatgat caccaccacc atcatcatgc
      541 agatgaagta tttacaagct ggggaaaaga aacacctaag aaatataata aggaagaatt
      601 ggatacgatc ttacagaaat tatccaaaga tgacagctat ggtatgatct taagatccaa
      661 aggtatttta cagacagaag atggatcatg ggttcagttt gatcttgttc caggagaata
      721 tgaaatccgt gaaggaagtg cggattacac aggaagaatc tgtgtgatcg gatcaaaatt
      781 aaaagaagat gaattagaag gtttattctt ctaaataaca ggaggaaaaa gaatgtacga
      841 attaccggca tatctggtat ggggattttt agaaagtgga aaatcaacac tgatcaaaga
      901 aacattaagc caggattatt ttaatgacgg cgagaaaaca atgatcctta cgtttgaaga
      961 aggagaagtc gaatacgata aagagatgct agagaagaca aattcatttg tggtgaatat
     1021 tgaaaatatg gaagatttca caaaagaatt tgtcagagga tgtcagagaa attattatcc
     1081 agaccgtgtc atgatcgaat acaatggaat gtactccatt gacgatctga tggatgtagt
     1141 agatgaaaca gatcttgagt tatatcaggt gatcgtaaca gttgatgcat ctacagcaga
     1201 tttgtatttg aaaaatatga aatctatgtt tatggaaatg tttaagatgg cagatcttgt
     1261 catttttaac cgctgtgatg acaatacgaa tatgggaagt ttccgaagaa gcatcaaagc
     1321 agtcaatcca agagcacagg taggtttcga acgtgccgat ggaaaagaga tggcacagga
     1381 tgaactgctg ccatatgatg tgaatgcaga tgtgatcaaa gtcgaagatg aagattatgg
     1441 aatctggtat atcgatgcga tggaacgtcc agaggtttac gaaggaaaga ctgtggaaat
     1501 gcgtgttcag gtccagcgca gtccaaagat gccaccggga atgatcattc ctggacgtaa
     1561 agcaatgaca tgttgtgaag atgatatgac atttattgga tatctgtgta gattaaacca
     1621 gacgaaatcc aagacattaa agagaattct caataacgaa tgggtaacat taacagcaca
     1681 gatccattca gaatacaatg aagcatatga taagacaatg ccaatcttaa cagcgatcag
     1741 aatcgaaaaa tcagaaccgc caaaagaaca attggtatat ttctagattt taaggacata
     1801 gcaaccctgc cccaagcata cactgtaaaa acaacattta cagggggctt ttccattgaa
     1861 gaaatacatg gaagaaagag tcattgaaag tgcgatctac atcattgaac ataatgctac
     1921 agtacgccag acagccaagc agttcggcgt cagcaaaagc acagtacata aagatgtgac
     1981 agaacggtta gaaacgatca acaaaacact tgcc
//
LOCUS       DAOMUE010000335         2013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_15845_length_2013_cov_1.708376, whole genome shotgun sequence.
ACCESSION   DAOMUE010000335 DAOMUE010000000
VERSION     DAOMUE010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15845_length_2013_cov_1.708376"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PLC71_10715"
     CDS             complement(<1..1078)
                     /locus_tag="PLC71_10715"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRM03147.1"
                     /translation="MKRLVLAEKPSVGRDIARVLGCKKETHSYIEGNDHIVTWALGHL
                     VSLADPEQYGKEYKEWNMDVLPMMPKHWKLTVLKKTSKQFQTVKKLLLRNDIKDVIIA
                     TDAGREGELVARWILQMSGNKKPIYRLWISSVTDKAIKDGFAHLKPGKEYDDLFRAAR
                     SRAEADWLVGINATRALTCKYNAQLSCGRVQTPTLAMIMNREQEIKSFKPQKYYGYKI
                     FATGMNFTWENQKGNQRISDKKWAEELGKKLKGHDLVITEVSKKEKKNYAPELYDLTT
                     LQKEASKRYGFSPKQTLNIMQSLYEHHKVLTYPRTDSRYLTNDMTDTLKDRIKACQNG
                     SYKKAAAALLRKEIKASSRFINDKK"
     gene            1204..1740
                     /gene="yfcE"
                     /locus_tag="PLC71_10720"
     CDS             1204..1740
                     /gene="yfcE"
                     /locus_tag="PLC71_10720"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HRM03148.1"
                     /translation="MKLMIASDIHGSAFYCKQLIEAYKQEQPDKLLLLGDLLYHGPRN
                     DLPKDYAPKQVIEMLNAISDQLICVRGNCEAEVDQMVLNFPVLSKSCILYIDGQTIYA
                     THGHINSPANPPKLHKGDILLTGHTHIPAWEEYDNFLYLNPGSVSIPKEGSGHGYMIY
                     EDKKFLWKTLKGDVYHTL"
BASE COUNT          570 a          424 c          307 g          712 t
ORIGIN      
        1 cttttttatc attgataaaa cggctgcttg ctttgatctc ttttcttaat aacgctgctg
       61 ctgccttttt gtatgatcca ttctggcatg ctttaatcct gtcttttaat gtgtctgtca
      121 tatcattggt taaatatctg gaatctgttc ttggatacgt caatacttta tgatgctcat
      181 aaagactctg catgatattt aatgtctgtt ttggtgaaaa gccgtaacgt ttactcgctt
      241 ctttctgcaa agttgtcaga tcataaagtt ctggagcata gttctttttc tcttttttag
      301 aaacctctgt gatcacaagg tcatgtccct ttaatttctt gccaagttct tctgcccact
      361 tcttgtcaga aattctctga tttcctttct gattctccca tgtgaaattc attcctgttg
      421 caaaaatctt ataaccgtaa tatttctgcg gtttgaaact cttaatttcc tgctctcggt
      481 tcatgatcat tgccaaagtt ggggtctgca cacgcccaca agataactga gcattgtatt
      541 tacatgtcaa agctcttgtc gcattgatcc cgactaacca gtctgcttca gctcttgatc
      601 ttgctgcacg gaagagatca tcatattctt ttcccggttt taaatgtgca aaaccatctt
      661 tgatcgcctt gtctgttacg gaagatatcc acaaacggta gattggtttc ttatttccag
      721 acatctgtaa gatccatctg gcaactaatt ctccttctct tcctgcatcg gttgcaatga
      781 tcacgtcttt gatatcattt cgaaggagta atttctttac cgtctggaac tgtttcgatg
      841 tcttctttaa caccgttaat ttccagtgtt ttggcatcat tggaagaaca tccatattcc
      901 attctttata ttcttttcca tactgctctg gatctgccag tgatactaaa tgtcctaatg
      961 cccacgttac aatatgatca tttccttcga tataactatg cgtttccttc ttacagccaa
     1021 gaactctcgc aatatcacgc ccaaccgaag gcttttctgc taatactaat cgtttcattt
     1081 tattatttaa acctttctaa aattgtcatc tcaaatttat tatcccgacc attttaacat
     1141 attttgtggt atactggaat aaagatattt tatcgtattt ttacagaatt gaggtattta
     1201 catatgaaat taatgatcgc atccgatatt cacggatctg ctttttactg taaacaatta
     1261 atcgaagcat ataaacaaga acagcctgat aaactacttc ttttaggaga tttactttat
     1321 catggcccaa gaaatgacct tcctaaagat tacgcaccaa aacaagtcat cgaaatgcta
     1381 aacgctatca gcgaccaatt gatctgtgtc agaggaaact gcgaagccga agtcgaccag
     1441 atggtactaa acttccctgt cctaagcaaa tcctgcatcc tatacatcga tggacaaacc
     1501 atctacgcaa cccacgggca cataaactct ccagcaaatc ctccaaaatt acataaagga
     1561 gatattctgt tgactggcca tacccatatc ccagcctggg aagaatatga taacttttta
     1621 tatttaaatc caggatctgt gtccatccca aaagaaggat ccggacatgg gtatatgatt
     1681 tacgaagata aaaagttttt atggaaaacc ttaaaaggtg acgtatatca tacactttaa
     1741 tttttttgtt ttcttatcac atatcttaat ggtattctat aaccttcatg tatataaaac
     1801 aaataaaaaa gagatggaat acgaaaacga ttgaatttca tggcggatca gcgttttgca
     1861 aaaaatccgt catgaaattc atgtttcgta ttccatctct tttttatttg ttttatatac
     1921 tagaagatta tatagtatca tttggatttc ccatataaat tcatcctcta tttctttctt
     1981 aacccttaat gatatcatcg atcgtgtcaa acg
//
LOCUS       DAOMUE010000336         2002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_15961_length_2002_cov_1.962506, whole genome shotgun sequence.
ACCESSION   DAOMUE010000336 DAOMUE010000000
VERSION     DAOMUE010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15961_length_2002_cov_1.962506"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLC71_10725"
     CDS             <1..694
                     /locus_tag="PLC71_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRM03149.1"
                     /translation="KEIKKVLVVLDITNEVVDYAIDQKADFILAHHPIIFSKINKCTS
                     DDFLQKKLLKLIRHDICAYGMHTCYDVCRMGDQVADRLNLKEIQGPVELSKSHNEKAF
                     GKGIGIVAKIEDGISVGEYAKKVKEAFSLENVMVFGNLDTKISRLAVVPGSGRSMISE
                     AKSTGADVFLTGDIGHHEGLDAVDMGMCVIDAGHYGLEQVFIDDMKDLIRTHFSDMNV
                     ITYKAGSPYKVV"
     gene            727..>2002
                     /locus_tag="PLC71_10730"
     CDS             727..>2002
                     /locus_tag="PLC71_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRM03150.1"
                     /translation="MIKATVNQSIYEVKEGTTLSDLAKQVQLPQEPIILLAYMDGKLR
                     ELFTPMTKDCHVRFVTLKEQAGYMAYKRTATLMFLKACEDLLGTGATTKIALDYSIGN
                     SIFCDFLEDRVIDDAFAQSIQKRMEELAKANLPITKRSLDTDQAAKYFDRIGFKGKKE
                     LFQFRRESKMNIYSLDGYDNYFYGYMAPSTGYISAFLVSAYQHGVVLQIPKRKQTEEI
                     VPFTPQPKLFHVMQRSREWTKTMGVDTVGALNDEITHGNINHLILLQEGLQEKLLADI
                     ADEIVSKNKRIILIAGPSSSGKTTFSHRLSIQLQIAGLTPHPVSMDDYFLDRELSPRD
                     ENGNYNFETIASLDVDLLTKHINQLLDGEEIDVPSYNFITGKREYHGHKLKIGEKDVL
                     VMEGIHGLNGTLTNEIPEDAKYRIYVSALNQIN"
BASE COUNT          696 a          310 c          454 g          542 t
ORIGIN      
        1 taaagagata aagaaagttt tagttgtatt agatatcaca aatgaagttg tagattatgc
       61 aattgatcaa aaggcagatt ttatccttgc acatcatccg atcatttttt caaagatcaa
      121 caaatgcacg agtgatgatt tccttcagaa gaaattatta aaactgatcc gccacgatat
      181 atgtgcatat ggaatgcata catgttacga tgtctgcaga atgggagatc aagtagcaga
      241 tcgcttgaat ttgaaggaaa tacagggacc agttgagcta tctaaaagcc acaatgagaa
      301 agcgtttgga aaaggaatag ggattgttgc caagattgaa gatgggatca gtgttggaga
      361 atacgcaaag aaagtaaaag aagcattttc tttggaaaat gtcatggtat ttggcaatct
      421 agatacaaag atctctagac ttgcagttgt tccaggatca gggagaagta tgatcagtga
      481 agcgaaaagt acaggagcag atgtattttt aactggagat atcgggcatc atgaaggcct
      541 tgatgcagtc gatatgggaa tgtgtgtgat cgatgcagga cattatggat tagaacaggt
      601 atttattgat gatatgaaag atctgatcag gactcatttc agtgatatga atgtgatcac
      661 atataaggca ggaagccctt ataaagttgt ttaaatagta gttatataaa aaggaagtga
      721 tggcgtatga tcaaagccac agtgaatcaa agcatttatg aagtaaaaga aggaacaaca
      781 ctttctgatc ttgcgaagca ggtacagctg cctcaggagc cgatcatttt gcttgcctat
      841 atggatggaa agttaagaga attatttact cctatgacaa aagactgcca tgtgcgtttt
      901 gtaacattaa aagaacaggc agggtatatg gcatataaac gaactgccac gcttatgttt
      961 ctgaaagcat gcgaagatct gcttggaact ggtgcaacga caaagatcgc attagattat
     1021 tcaatcggaa atagtatttt ttgcgatttc ctagaagatc gtgtgataga tgatgctttt
     1081 gcgcaatcca ttcaaaaacg tatggaagag ctagccaaag cgaatcttcc aatcacaaaa
     1141 agatcgctgg acacagatca ggctgcaaaa tattttgata ggataggctt taaggggaag
     1201 aaagaattat ttcagttccg cagggaatca aagatgaata tctactcgtt ggatggatat
     1261 gataattatt tttatggata tatggcacca tcgacaggat atatttcagc atttttggtg
     1321 tctgcatatc agcatggggt agtacttcag attcccaaaa ggaaacagac agaggaaatc
     1381 gtaccattta caccacagcc aaaacttttc catgtaatgc agcgttccag agaatggacg
     1441 aagacgatgg gtgttgacac agtaggtgcc ctaaatgacg agatcacaca tggaaatatc
     1501 aatcatttga ttttattaca agaaggattg caggaaaaac ttcttgcaga tattgcggat
     1561 gagatcgtaa gcaagaacaa gagaatcatc ctgatcgcag ggccgtcatc ctcagggaag
     1621 acaacatttt cccatagatt atccattcaa ttgcagattg ctggattaac accacaccca
     1681 gtttctatgg atgattattt cttagacagg gaattgtctc caagagatga aaatggaaat
     1741 tataattttg agacgatcgc atcgttggat gtggatcttt tgacaaaaca tatcaatcaa
     1801 ttgctagatg gggaagagat tgatgtgcca agttataact ttatcacggg aaaaagagaa
     1861 taccacggac acaaattaaa gattggagaa aaagatgtcc tggtcatgga gggaatccat
     1921 ggattaaatg gtactcttac gaatgaaata ccagaagatg caaaatatag aatctatgtc
     1981 agtgcattga atcagatcaa ct
//
LOCUS       DAOMUE010000337         1976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_16259_length_1976_cov_1.689224, whole genome shotgun sequence.
ACCESSION   DAOMUE010000337 DAOMUE010000000
VERSION     DAOMUE010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16259_length_1976_cov_1.689224"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="PLC71_10735"
     CDS             complement(<1..1907)
                     /locus_tag="PLC71_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRM03151.1"
                     /translation="MRKRVLTYSLAVAMAVAMAVPQTVILSTGIVQVKAANTETGLKT
                     GIASGTENYIKFTEMDKYASTSSDYCSKINKVVVNDKEYPVYDEEGKDNYYSMTYSDG
                     LCIYMGSIKDGENTIIVSANGYKDKKIIVNVDKTAKTVTFVSQEDLGSGDAVIDKNAL
                     NNMITAAKAYTKESASDEKWNAFQEAIKTAEDVYNNESATQDQIDQAVAALREAIDTF
                     KKEDPTLNPTEDGVYTVTLKATKQGSKEPSSLGGYFEGKAKLTVKDGKMKLSMLNISM
                     AKYLLDFTLETDGTFAKATKEAYGDANSDGSYDAYEYTMSIKDLSKIHTAAALVSVMG
                     SESDKGNYDKYMKADITFTSLEKGWKGYDKKDANQTLVDALIEAGADTNNDGEITQEE
                     LAAFNGDEGSLDLSNKNLSNIDLLKGLSDKIKELDLSGNKITEIPEGFFDNMTNLEYV
                     DLNSNHIKALPENAFKNTKKLNWINIRANNLSGLDKLVYLELDNNSITSVEAGALDGD
                     TSLKQLSISGNELNALPDHLLDDAGDTINFIELSNNEFVKLPNCINAATKKLRKISAF
                     NNALEDISNIDFTKMSNLEEVNFYKNYIAQVPDGTFAKNKKLYSVDFHDNQISNITEK
                     AFPETFENDYDG"
BASE COUNT          516 a          388 c          271 g          801 t
ORIGIN      
        1 ccatcataat cgttttcaaa tgtttcagga aatgccttct ctgtaatatt agagatctga
       61 ttatcatgga aatctacaga atatagtttt ttattctttg caaatgttcc atctggcact
      121 tgagcaatat agttcttgta gaaattaacc tcctcaagat ttgacatctt tgtaaaatca
      181 atattcgaaa tgtcttctaa tgcattattg aatgcactga tctttcgtaa tttctttgtt
      241 gctgcattga tacagtttgg aagttttaca aactcgttat tggataattc aataaaatta
      301 atcgtatctc cagcatcatc taagagatga tctggtaatg catttaattc atttcctgaa
      361 atggatagct gctttagact tgtatctcca tccagtgccc ccgcctctac agacgtgata
      421 ctattattat ccagttcaag atatactaat ttatccaatc ctgacagatt atttgctctt
      481 atattgatcc aattcaattt ttttgtattt ttaaacgcat tttctggaag agcttttata
      541 tgatttgaat tcaaatctac atactcaaga tttgtcatat tatcaaaaaa tccctctgga
      601 atttctgtga tcttatttcc tgaaagatcc aattctttaa tcttatctga taaacctttt
      661 aacagatcga tattactcaa attcttgttt gataaatcca gacttccttc gtctccatta
      721 aatgctgcta attcttcctg ggtaatttct ccatcattgt tcgtatcagc tcctgcttcg
      781 atcaatgcat caaccagggt ctggttcgca tcttttttat catatccttt ccatcctttt
      841 tccaaagatg tgaatgtgat atccgctttc atatatttgt catagttacc tttatcagat
      901 tcagatccca taacggatac taaagctgcc gctgtatgta ttttgctcag atctttaatg
      961 ctcattgtat attcatatgc atcataagaa ccatcactat ttgcatcccc atatgcttct
     1021 tttgttgctt tggcaaacgt tccatctgtc tctaatgtga agtcaagtaa gtatttcgcc
     1081 atggagatat ttaacatgga taatttcatt tttccatctt ttactgtcag ttttgctttt
     1141 ccttcaaaat atccacctaa ggaagagggt tccttgcttc cttgttttgt tgctttaagt
     1201 gtaactgtat atacaccatc ttctgttgga tttaatgttg gatcttcttt tttaaacgta
     1261 tcaattgctt cccttaatgc agcaactgcc tgatcgatct gatcctgagt tgcagattca
     1321 ttattatata catcttctgc tgttttgatc gcttcctgga atgcattcca tttttcatca
     1381 gatgccgatt ctttcgtata tgcttttgct gctgtgatca tattatttaa cgcattttta
     1441 tcgatcactg catctcctga tcccaaatct tcctgtgaaa cgaaagttac tgtttttgct
     1501 gttttgtcga cattcacaat gatcttttta tctttgtaac catttgctga aacaatgatc
     1561 gtattttcgc catctttgat tgatcccata tatatacaaa gtccatcaga atatgtcatt
     1621 gaataataat tatcttttcc ttcttcatca taaactggat attccttgtc gttcactaca
     1681 actttattga tcttactgca ataatctgaa gaagtagatg catatttatc catttcagta
     1741 aatttaatat aattttctgt tcctgacgca attcctgttt ttaatccagt ttctgtattt
     1801 gccgctttta cctgtacaat acctgttgat aagattactg tctgtggaac tgccattgct
     1861 actgccattg ctactgctag tgaatatgtt aatactcttt ttctcatcat tttctccttt
     1921 gtagattatt ttttgatctt aatttttttg atcgttgaaa attttccgta tacttt
//
LOCUS       DAOMUE010000338         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_16297_length_1972_cov_2.802817, whole genome shotgun sequence.
ACCESSION   DAOMUE010000338 DAOMUE010000000
VERSION     DAOMUE010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16297_length_1972_cov_2.802817"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="PLC71_10740"
     CDS             complement(<1..499)
                     /locus_tag="PLC71_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRM03152.1"
                     /translation="MGSNRYVGTQTEKNLLEAFSGESQARNKYTYFASVAKKEGYEQM
                     SALFLKTANNEKEHAKMWFKELAGIGDTKENLAAAAEGENYEWTDMYEGFAKTAEEEG
                     FPELAAKFRAVGEIEKHHEERYRALLKNIETSQVFEKSEVKVWECRNCGHIVVGTKAP
                     EVCPVC"
     gene            complement(563..925)
                     /locus_tag="PLC71_10745"
     CDS             complement(563..925)
                     /locus_tag="PLC71_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HRM03153.1"
                     /translation="MAKFYRSEDKTVLLEVFGAGSSIENLKNLKANTTDAAVEKHLPV
                     VTQEGNKITVAVSSVEHPMLPEHYIMGVYIETKNGGQLHKFAPGDKPEAVFMLADGDE
                     FIAAYEYCNLHGLWKSEV"
     gene            complement(964..1119)
                     /locus_tag="PLC71_10750"
     CDS             complement(964..1119)
                     /locus_tag="PLC71_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRM03154.1"
                     /translation="MKYVCDVCGWEYDEEKGYPEGGIAPGTKWEDVPEDFECPLCFVG
                     KDQFSKE"
     gene            1385..1744
                     /locus_tag="PLC71_10755"
     CDS             1385..1744
                     /locus_tag="PLC71_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HRM03155.1"
                     /translation="MEGKITKIFNAADQIQAEMIHDHLKQNGIETMSKQPGTGEYMSI
                     AMGYSVYGTDIYVREDQKAKAEALINELLYDNDYNTFEDEDIKLPWWQNKVIVAKCIL
                     GFFILVIIIMIASQIFQ"
BASE COUNT          580 a          399 c          271 g          722 t
ORIGIN      
        1 cacacacagg acatacttcc ggtgcttttg ttccaactac gatatgtcca caattgcgac
       61 attcccatac tttcacttca cttttttcaa atacttgtga ggtctcgata ttcttaagaa
      121 gtgcacgata tctttcttca tgatgcttct caatctctcc gactgctctg aatttggcag
      181 caagttctgg aaaaccttct tcttctgcag tctttgcaaa tccttcatac atatctgtcc
      241 attcgtaatt ttcaccttca gctgctgcag caagattttc ctttgtatct ccaattcctg
      301 ctaattcttt aaaccacatt ttggcatgtt ctttctcatt atttgccgtc ttaaggaaca
      361 atgcagacat ctgctcatat ccttctttct ttgcaacgga tgcgaaataa gtgtacttat
      421 ttcttgcctg tgattctcct gaaaatgctt ctaataaatt cttctctgtc tgtgttccta
      481 catatctatt acttcccatg attcttctcc ttactgattc ctttacaatc actttatata
      541 tttccctaga aattgataat ttctatactt cagatttcca taatccatgc agattacaat
      601 actcatatgc tgctatgaat tcatctccat cggctaacat aaataccgcc tctggtttat
      661 cccctggtgc aaatttatgc agctgcccac cattctttgt ttcaatatat actcccataa
      721 tataatgttc tggaagcatt ggatgctcta cggaagatac agctactgtg atcttatttc
      781 cttcctgtgt cacaactggt aaatgttttt ctactgcagc atctgttgtg tttgctttta
      841 aatttttcaa attctctatg gaacttcctg ctccaaatac ttctaataat actgttttgt
      901 cttctgaacg ataaaattta gccataactt cttctccctt ctactacaat atatctataa
      961 aaattattct tttgaaaatt gatctttccc tacaaaacat aatggacatt caaaatcttc
     1021 tggaacatct tcccattttg ttcctggagc gattcctcct tctggatatc ctttttcttc
     1081 atcatattcc catccacata catcacatac gtatttcatt gtttcatcct ccttatactt
     1141 aaatcgataa taattactgt tattatattt atataataat acatgtgaga aaaaatgtca
     1201 ataatatttt ttagaatttg aatagtgata tttctttcta tgaattatgt ccaatttttg
     1261 ttataattaa ttcatactta ttgacacgcc gggtacatca gagcgtgaca aaatttactt
     1321 ttgatactct catcattaat atacaaagtt actgaacatt tactcaataa gggaggtggc
     1381 aattatggag ggtaagatta caaaaatatt taacgcagct gaccagattc aggctgagat
     1441 gattcatgat catttaaaac aaaacggaat tgaaaccatg tccaaacaac ctggaacagg
     1501 agaatatatg agtattgcaa tggggtattc tgtttatgga acggatattt atgttagaga
     1561 agatcagaaa gctaaggcag aagctttgat caatgaactg ttatacgata atgactacaa
     1621 tacattcgaa gatgaagata tcaaattgcc ttggtggcaa aataaagtga ttgttgcaaa
     1681 atgcatttta ggatttttta ttcttgtgat cattatcatg atcgcaagtc aaatcttcca
     1741 ataaaacaat aaatttaaga aagaacaggt atgcataagg aattcattaa attccttatg
     1801 catacctgtt ctttgttgtt gttcattatt tatatttagt ttatagttac cttatagtta
     1861 atcaaatttc tgaaatacac ccttaagtgc tggataaatt tctctaaact gctggtactt
     1921 cgcctcatac ttcgcaacaa gcttgtcatc aggttccacc gtatccacaa ct
//
LOCUS       DAOMUE010000339         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_16301_length_1972_cov_2.010955, whole genome shotgun sequence.
ACCESSION   DAOMUE010000339 DAOMUE010000000
VERSION     DAOMUE010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16301_length_1972_cov_2.010955"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="PLC71_10760"
     CDS             <1..304
                     /locus_tag="PLC71_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HRM03156.1"
                     /translation="LFKLITAIATVAGILYVARDQIKAIIAKIKEVSDDDFDDDFDDE
                     SFDDDSFDDDEIFPEPSKDDRDYVSINITDTDDDPSVAEEETQETETPSEESDEKE"
     gene            complement(365..1876)
                     /locus_tag="PLC71_10765"
     CDS             complement(365..1876)
                     /locus_tag="PLC71_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03157.1"
                     /translation="MRTAKNILLFVFILIISLNVTVHAQENGEKTENPVKVTQESEND
                     KDVPFTNSKGETVYTLSATTRDREGAIMMPKDRILCLTSETVDLSDIEVLIDDSNEQA
                     KKDLEVEVISASKNTAKICVNFSNRYVSTDLNWDGKILVKKGNDVIREIAIAYMTPYI
                     RDVHDTGFQTLSSWSGTWGVLDGEQYRSSGIVDVYQENAQISFTYQSQGNQGVKAFHL
                     YQSGNYTFSDGSYDIKAQNQGIKAYNTVNIRLKPTYIKQLKEELVNDLYQYSLDTKQC
                     VFDDLMIEYEDGCIIGEVTDFVRLGHQATIKPLDKMTLKANIKSTSKIFLQWKKLDRV
                     SGYAIYRYDSGKQRYEKIKTVSTQETSYTDSKLKSGKTYYYKIVPYRSITYTNAGNLC
                     KVVNGSSSNIAKCITKPGKPVIKAKRSGRRIKVKIKKVSGASGYKVYLRKGKKGRYKV
                     VKTYKGNRPRTYKSRKLKRKKMYYVKVRAYKTYQSKKYFGKYSKTKKVKIPKK"
BASE COUNT          551 a          427 c          292 g          702 t
ORIGIN      
        1 cctttttaaa ctgatcactg ccatcgctac cgttgctggt attttatatg ttgcccgtga
       61 tcagatcaaa gcgatcattg caaagattaa agaagtatcc gatgatgatt ttgacgatga
      121 tttcgatgac gaatcttttg atgatgattc ttttgatgat gatgagattt tccctgaacc
      181 atcaaaagac gacagagatt atgtttctat caacatcaca gatactgatg atgatccatc
      241 cgtagctgaa gaagaaacac aagaaacaga aactccatcc gaagaatctg acgaaaaaga
      301 ataattcgta aatcgaaaaa gacagatgag aagaatcacc tcatctgtct tttttctcac
      361 atcatcactt ctttggaatt ttgacttttt tcgtttttga atattttcca aagtatttct
      421 tggattgata tgttttatat gcacgtactt tcacataata catctttttt ctttttaact
      481 ttcgactctt atacgtacgg ggccgattgc ctttatatgt tttaacaact ttataccggc
      541 ctttctttcc cttccttaaa tatactttat atccactggc tccgcttact tttttaatct
      601 ttacttttat tcttcttccc gatctctttg cctttatcac tggttttccc ggttttgtga
      661 tacattttgc gatattggaa gatgaaccat tcacaacctt gcataaattt ccagcatttg
      721 tatatgtaat ggaacgatat ggcacaattt tataataata tgtcttccca gattttaact
      781 tagaatctgt ataggaagtc tcctgtgttg agactgtttt tatcttttca tatcgctgtt
      841 ttccagaatc ataacgatag atcgcataac cactgacacg atcaagtttc ttccactgaa
      901 gaaaaatctt tgatgtagat tttatatttg cctttaatgt cattttatct aacggcttga
      961 tcgttgcctg atgtcctagt ctcacaaaat ctgtgacttc tccgatgata catccgtctt
     1021 catattcaat catcagatca tcgaacacac actgctttgt atctaaactg tattgataca
     1081 ggtcattgac cagttcttct tttaactgct tgatataagt tggtttcaga cggatattca
     1141 ctgtattata agccttgata ccttgattct gagccttgat atcgtatgat ccatccgaaa
     1201 atgtataatt cccactttgg tataaatgaa atgctttcac tccctgattt ccttgtgact
     1261 gataggtaaa agaaatctgt gcattttcct gatacacatc aacaatccca gaggagcgat
     1321 attgctctcc atctaaaacg ccccatgtac cagaccatga acttaacgtt tgaaatcctg
     1381 tatcatgcac atctcttatg tatggtgtca tgtaagcgat cgcaatctcc ctgatcacgt
     1441 cattgccttt ttttacaaga atcttcccat cccagtttaa atcagtcgaa acataccgat
     1501 tcgaaaaatt cacacaaatc tttgcggtat tcttagacgc agatataacc tcaacctcaa
     1561 gatccttttt ggcctgttca tttgaatcat cgatcaaaac ttcgatatca cttagatcaa
     1621 ccgtctccga agtaagacat aaaatacggt ctttcggcat catgatcgca ccttctctgt
     1681 cccttgtagt tgcagacaat gtatatactg tctctccttt actattcgta aacggcacat
     1741 ctttatcatt ctcagattcc tgtgttacct tgaccggatt ttctgtcttc tctccattct
     1801 cctgtgcatg tactgttaca ttcaaactta tgatcagtat gaagacaaac aaaagtatat
     1861 tcttcgctgt tctcatcagt tttcctccat tattcaaaaa gaatcctgca aagcaggatt
     1921 ctccacattt aaaacggctt atcttgttgc ctcatttaac attctcttaa ga
//
LOCUS       DAOMUE010000340         1969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_16349_length_1969_cov_1.464472, whole genome shotgun sequence.
ACCESSION   DAOMUE010000340 DAOMUE010000000
VERSION     DAOMUE010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16349_length_1969_cov_1.464472"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>273
                     /locus_tag="PLC71_10770"
     CDS             <1..>273
                     /locus_tag="PLC71_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRM03158.1"
                     /translation="FLKREEEYTTGLQDGFAIPHAKTEYAKETAVFFVKCKNQLDWGT
                     LDDTKVNYAFALIVPKEAAGNEHLMMISQLATNLLEDEFRDEVKSAT"
     assembly_gap    274..283
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            338..649
                     /locus_tag="PLC71_10775"
     CDS             338..649
                     /locus_tag="PLC71_10775"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIB"
                     /protein_id="HRM03159.1"
                     /translation="MKIVGISACPAGLAHTPMAAKALEKAGKSLGYDVKMEQQGSMGQ
                     VNTITDEEAQAADLVIIASDQKITGMERFANKKVIRVDINICIKAPEAVLKKCVAAVS
                     K"
     gene            702..1667
                     /locus_tag="PLC71_10780"
     CDS             702..1667
                     /locus_tag="PLC71_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HRM03160.1"
                     /translation="MKQFLKDAKGHLMTGIGYMLPLIIGASLVVAIPKLIALCFGITS
                     LDPYADGTGIWHIMKLIENVGWTGIGMVNTVLAGFIAYSIADKPAIGAGLIGGAVASS
                     TYAGFLGAVIAAFIAGYSVKWAKKHIKLPESMNSVMPLVVCPLIATGLVAVIMGVILA
                     TPLAAINTWLVKWISSMCQNQSSQLVMALILGAMIASDMGGPINKSAWMAGNALMAEG
                     IYQPNVFINAAICMPPLAYAIATVIRKNRFSEAFRETGKSNWAMGFVGITEGAIPFTL
                     VKPQFLIPINMFGAAVGTAVISLLGAKGDIPPVGGIYGFVSITNG"
BASE COUNT          712 a          291 c          417 g          539 t
ORIGIN      
        1 tttcttaaaa agagaagaag aatatacaac aggacttcag gatggatttg caattccaca
       61 tgcaaagaca gaatatgcaa aagaaacagc tgtatttttt gtaaaatgca aaaatcagtt
      121 agattgggga acgcttgatg acacgaaagt aaattatgca tttgcattga tcgttccaaa
      181 agaagcagca ggaaatgaac atttaatgat gatttctcaa ttggcaacga atctgttaga
      241 agatgaattt agagatgaag taaaatctgc aacnnnnnnn nnnataagtc agttttaaag
      301 gaatatatac taaaaaatat gaaggagaat aattaatatg aaaattgtag gaatttcagc
      361 ttgtccagca ggacttgcac acacaccaat ggcagcaaaa gcattagaaa aagcaggaaa
      421 gtcattagga tatgacgtaa aaatggaaca gcaggggagt atgggacagg taaatacaat
      481 tactgacgaa gaagcacagg cagcagattt agttatcatt gcttctgatc agaaaatcac
      541 aggaatggaa cgtttcgcaa ataaaaaagt tatccgtgta gatatcaata tttgcattaa
      601 agcacctgaa gcagtactga aaaaatgtgt tgcagcagtc agcaaataag agctgtgaca
      661 taaattagta acataaaggc aaatgaaaaa aggagattaa aatgaaacaa tttttaaaag
      721 atgcaaaagg tcatttgatg accggtattg gatatatgct tccattaatc attggagcat
      781 ccttagtagt tgcaattcca aaactgatcg cattatgttt tggtatcact tcattagatc
      841 catatgcaga tggaacagga atctggcata tcatgaagct gattgaaaac gttggttgga
      901 ctggtattgg aatggttaat acagtattag caggattcat tgcttacagt attgcggata
      961 aaccagcaat tggagcaggt ttgatcggtg gtgcagttgc atctagtaca tatgcaggat
     1021 tcttaggtgc tgtcattgca gcgtttattg ctgggtattc agtaaaatgg gcgaaaaaac
     1081 atattaaatt accagaatca atgaattcag taatgccact ggttgtttgc ccattgatcg
     1141 cgacaggatt agtagcagtt atcatgggtg ttatacttgc aacaccactt gcagcaatta
     1201 acacatggct tgtaaaatgg atcagttcaa tgtgccagaa ccagagctca cagttagtaa
     1261 tggcattaat tcttggagca atgatcgcca gtgatatggg tggaccaatc aataaatcag
     1321 catggatggc aggaaacgca ttaatggcag aaggaattta tcagccaaac gtatttatca
     1381 atgcagcaat ctgtatgcct ccacttgcat atgcaatcgc aacagttatc agaaagaata
     1441 gattctcaga agcatttaga gaaacaggta aatcaaactg ggcaatggga tttgtaggaa
     1501 ttacagaagg agcaattcca tttacacttg taaaacctca gttcttaatt ccaattaaca
     1561 tgtttggtgc agcagtaggt acagcagtca tttcactttt aggagcaaaa ggtgatattc
     1621 ctccagttgg tggaatttat ggatttgttt caattacaaa tggttgagct taccttgtag
     1681 gtattcttgt aggagcactg atcatcgcat tacttgctac attagtagtt gatttcactg
     1741 aaggagatga aggtgaatca gaagatattg atgttgatga aattgaaatc actttcgaag
     1801 atatcaaata gaaaataaaa taatagaaaa taagtttaca acaaataaat aactcagtaa
     1861 atgggcaggg gctaagtttt ttgcctctgc ccattttaca ataagaaagg aatcagaatg
     1921 aaaagaaaaa tacacgtaat accacattca cattgggata gagaatggt
//
LOCUS       DAOMUE010000341         1968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_16357_length_1968_cov_2.107162, whole genome shotgun sequence.
ACCESSION   DAOMUE010000341 DAOMUE010000000
VERSION     DAOMUE010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16357_length_1968_cov_2.107162"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PLC71_10785"
     CDS             <1..233
                     /locus_tag="PLC71_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HRM03161.1"
                     /translation="VACVGIDFVKLGKQTGKMAAKILKGEEKAKDMKFETFNNGDVII
                     NTAAAKKINMTISKDVQKQAKQTFDKISAPEK"
     gene            252..>1017
                     /locus_tag="PLC71_10790"
     CDS             252..>1017
                     /locus_tag="PLC71_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM03162.1"
                     /translation="MEQLIIGVFEQGFIYAIMALGVYITYKILDFPDLSVDSTFPLGA
                     AVTTVMLSSGKNPLLALIAATAAGAIAGMVTGIIHVKFKVRDLLSGIIVMTGLYTLNL
                     RIVGGSANVPLFNYKSVFDNDFINGIFPEALAPYKTVIIIFIIMIIGKILLDLYLKTK
                     SGYLLRAVGDNETIVTSLAKDSGFVKIVGLAISNGLVALAGSVMCQQQRFFEISMGTG
                     TIVIGLASVIIGTNVFKGNLIKATTAVVIGSVIYKAC"
     assembly_gap    1018..1062
                     /estimated_length=45
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1147..1920
                     /locus_tag="PLC71_10795"
     CDS             1147..1920
                     /locus_tag="PLC71_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM03163.1"
                     /translation="MIELKHIDKYYNIGTVNEVCLFKDFNLTVDDGDFISVIGSNGSG
                     KTSMLNLICGSLEAEAGNIILNGEDISHQKEYIRQRKIGRVYQNPAMGTCPSMTILEN
                     MSLADNKGKKFGLGFGTNKKRVDYYRELLSQLGLGLEDMMGSKVGSLSGGQRQAMALL
                     MTTMAPIEILILDEHTAALDPKTAELIMELTGKVVKEKKLTTIMVTHNLRYAVEYGNH
                     ILMMHQGHAVLDKAGEEKAKLVVDDLLDQFNQISIECGN"
BASE COUNT          692 a          312 c          407 g          512 t
ORIGIN      
        1 gtgtagcttg tgtcggaatt gacttcgtaa aattaggaaa acagacaggt aagatggcag
       61 ctaaaatctt aaaaggcgaa gaaaaagcaa aagatatgaa attcgaaaca tttaacaatg
      121 gagatgtcat cattaataca gcagcagcta agaagattaa catgacaatc agcaaagatg
      181 tgcagaaaca ggcaaaacag acatttgaca agatcagcgc accagaaaaa taatcaggag
      241 atgtaaatat tatggaacaa ttgatcatag gcgtatttga gcagggattt atctatgcga
      301 tcatggcgct tggcgtatat atcacataca aaatattaga ctttccggat ctgtccgtag
      361 acagtacttt cccattagga gcagcagtga caaccgtcat gctctcctca ggaaagaacc
      421 cactgcttgc attaattgca gcaacagcag ccggagcaat tgcaggaatg gtcacaggaa
      481 tcatacatgt aaaattcaaa gtcagagatt tattatccgg aattatcgtt atgacaggtc
      541 tttatacctt aaacttaaga attgtaggcg gaagcgcaaa tgttccatta tttaactaca
      601 aatccgtatt tgataatgac ttcatcaacg gaatcttccc agaagcatta gcaccatata
      661 agacggtgat cattattttt atcatcatga tcattggcaa aatattatta gacttatatt
      721 taaaaacaaa atcaggatac ttgttaagag ccgttggaga taacgagaca atcgtaacat
      781 ctcttgcaaa agacagtgga tttgtgaaga tcgtaggact tgcgatctca aacggattag
      841 ttgcattagc tggaagtgtt atgtgtcagc agcagagatt ctttgaaatc tctatgggaa
      901 ctggaacgat cgttatcgga cttgccagtg ttatcattgg aacaaacgta tttaaaggaa
      961 atcttatcaa agcaacaact gcagttgtca tcggttctgt gatctacaaa gcatgtgnnn
     1021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaacagact taaaattgat
     1081 cacagcagtc ttattcctga tcattctgat catcagtatg gaccgtaaga agaaggtgaa
     1141 aaaggcatga ttgaattaaa acatatcgac aaatattata atatcggaac agtcaatgaa
     1201 gtctgcctgt tcaaagactt caatctgacg gtagatgacg gagatttcat ttccgtgatc
     1261 ggaagtaatg gttcaggaaa gacatccatg ttaaatctga tctgcggaag cttagaagcg
     1321 gaagcaggaa atattatttt aaatggagaa gatatttctc atcaaaaaga gtatatcaga
     1381 cagagaaaga tcggacgtgt ttatcaaaat ccagcaatgg gaacctgccc atctatgaca
     1441 atccttgaaa atatgtctct tgcagacaat aaaggaaaga aatttggact tggatttggt
     1501 acaaataaga agagagttga ctattacaga gaattattaa gccaattagg acttggactg
     1561 gaagacatga tgggaagcaa agtaggttcc ttatcaggag gacagagaca ggcaatggca
     1621 cttcttatga caacgatggc accaattgag atcttaatac ttgatgaaca tactgctgca
     1681 cttgatccaa agacagcaga actgatcatg gagttgacag gaaaagttgt caaagaaaag
     1741 aaactgacaa cgatcatggt tacacataac ctgcgttatg ccgtagaata tggaaatcat
     1801 attcttatga tgcatcaggg acatgcggta cttgataaag ctggagaaga gaaagcaaaa
     1861 ttagttgttg atgatcttct ggatcagttt aatcagatca gtattgagtg tggaaattaa
     1921 atttttaaat tgtataacgg aaatgtatcg agtattcata aaaacata
//
LOCUS       DAOMUE010000342         1966 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_16383_length_1966_cov_1.997907, whole genome shotgun sequence.
ACCESSION   DAOMUE010000342 DAOMUE010000000
VERSION     DAOMUE010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16383_length_1966_cov_1.997907"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>657)
                     /gene="lgt"
                     /locus_tag="PLC71_10800"
     CDS             complement(<1..>657)
                     /gene="lgt"
                     /locus_tag="PLC71_10800"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRM03164.1"
                     /translation="FEIKYYGMIIATGFLLGLIVSYYAAKHENKFDPELVFDYLITMI
                     IPAIVGARLYYVIFSWDYYKNHLSEIIQIRNGGLAIYGGVIAGAITLYIFCKKKHLSM
                     LQFGDVAVKGLLVGQILGRWGNFFNREAFGSYTNSLFAMQIPTNFFIEHGRIDEIVSN
                     GLYDKATYVVSGNEAATFIQVHPTFLYESMWNLVLLLLIIFFFTKRKKFDGELIAIYC
                     "
     assembly_gap    658..710
                     /estimated_length=53
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            928..>1966
                     /gene="ychF"
                     /locus_tag="PLC71_10805"
     CDS             928..>1966
                     /gene="ychF"
                     /locus_tag="PLC71_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015528367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRM03165.1"
                     /translation="MKLGIVGLPNVGKSTLFNSLTKAGAESANYPFCTIDPNVGIVTV
                     PDARVDYLAEKYHSKKVIPAAIEFVDIAGLVKGASKGEGLGNQFLANIREVDAIVHVV
                     RCFDDGNIVHVDGSVNPLRDIETINFELIFSDIEVLDRRIAKSTKGSRNDKSLAHEVE
                     FLSKVKAHLEDGKLAKTFEVDDEEDQEILNSCNLLTIKPVIFAANVNEDELAGEDNDY
                     VKAVREYAADVDAKVFVVCAQIEQEISELDDDEKKEFLEDLGLEESGLEKLISASYSL
                     LGLISFLTSGEDETRAWTIKEGTKAPQAAGKIHTDFERGFICAEVVSFEDLKEYGTMQ
                     ACKEKGLVRQEG"
BASE COUNT          593 a          367 c          372 g          581 t
ORIGIN      
        1 tacaataaat ggcgatcaat tctccatcaa atttctttct cttcgtaaaa aagaaaatga
       61 tcaacagtag taaaacaaga ttccacatag attcatataa gaatgtcgga tgtacctgaa
      121 taaaggttgc tgcttcattt ccagatacaa cataagttgc cttatcatac aatccattgg
      181 atacgatctc atcaatcctt ccgtgttcaa taaagaaatt tgtcggaatc tgcattgcaa
      241 ataaactgtt ggtatatgat ccaaacgctt ctctgttaaa gaaattgccc catcgtccaa
      301 gaatctgacc gacaagcagc ccttttaccg ccacatctcc aaactgaagc atagataaat
      361 gtttcttctt gcagaagata tataatgtga ttgcacctgc gatcacacct ccataaattg
      421 caagtcctcc atttctgatc tgtatgatct ctgacaaatg attcttataa taatcccatg
      481 aaaagatcac ataatagagt cttgctccta cgatcgccgg aatgatcatc gtgatcagat
      541 agtcaaagac taattccgga tcaaatttat tttcatgttt tgctgcgtaa taagaaacga
      601 tcaatccaag caagaatcct gttgcaatga tcattccata atatttgatc tcaaaccnnn
      661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cctaaatgtg
      721 ggaatctgat ttgatgttcc atcacttttc ttcctttcta aagcctcgtt ttgtctcttc
      781 tttgttttgt tactcaaatc aaagatatct tatcccattt ctcttttctt ttcaagtcgc
      841 ttatggtaaa ctatctacag tatgaccttg gatatttttt caaggtctgt tttaagaaat
      901 acccatgaga aaggaaatta atagattatg aaattaggaa tcgttggatt accaaacgtc
      961 ggaaagagta cattatttaa ctctttaaca aaagccggtg ctgaatccgc taactatcca
     1021 ttctgtacga tcgaccctaa tgtgggaatc gtaacagttc cagatgctcg tgttgattat
     1081 ctggctgaga aataccattc aaagaaagtg atccctgcag cgatcgaatt cgttgatatc
     1141 gcaggtcttg tgaaaggagc ttccaaaggt gaaggtctcg gaaaccagtt ccttgcaaat
     1201 atccgtgaag tcgatgcgat cgttcacgtt gttcgttgtt ttgatgatgg aaatatcgtt
     1261 cacgttgatg gttctgtaaa cccacttcgt gatatcgaaa cgatcaactt cgagctgatc
     1321 ttctctgata tcgaagtgtt agaccgtcgt atcgcaaaat caacaaaagg atcaagaaat
     1381 gataaatctc ttgctcacga agttgaattc ttatctaaag tgaaagctca tctggaagat
     1441 ggaaagcttg ctaagacatt tgaagtcgat gatgaagaag atcaggaaat cttaaattcc
     1501 tgcaacctgt taacgatcaa accagtcatc tttgctgcga acgttaacga agatgaactt
     1561 gctggagaag ataacgatta tgtaaaagct gtccgtgagt atgctgcaga cgttgatgcg
     1621 aaagtattcg ttgtatgtgc tcagattgaa caggaaatct ctgaattaga tgatgatgag
     1681 aagaaagaat tcttagaaga tcttggatta gaagaatctg gtcttgaaaa actgatctct
     1741 gccagctact ctcttcttgg actgatcagt ttcttaactt ccggagaaga cgagacacgt
     1801 gcttggacga tcaaagaagg tacaaaggct cctcaggctg ctggtaagat ccatacagac
     1861 tttgagcgtg gatttatctg tgctgaggtt gtttcttttg aagatcttaa ggagtatggt
     1921 acaatgcagg cttgtaaaga gaaagggctt gtacgtcagg aaggga
//
LOCUS       DAOMUE010000343         1936 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_16724_length_1936_cov_1.689527, whole genome shotgun sequence.
ACCESSION   DAOMUE010000343 DAOMUE010000000
VERSION     DAOMUE010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16724_length_1936_cov_1.689527"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..776)
                     /locus_tag="PLC71_10810"
                     /pseudo
     CDS             complement(165..776)
                     /locus_tag="PLC71_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261689.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            complement(807..1936)
                     /locus_tag="PLC71_10815"
                     /pseudo
     CDS             complement(807..1936)
                     /locus_tag="PLC71_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393197.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
BASE COUNT          454 a          435 c          363 g          684 t
ORIGIN      
        1 cagcgatata atgctggtgc ttccagtgaa tgatcacagc cgacaaatgt tactttcacc
       61 cctaatctgc cgacagcata gcggaaggta tcgataaatt cgacctgatc tgccacgctt
      121 gaaaataaga tctttaagtg tcctgctttc ttgtatttgg aaaactggtc tttctttgtt
      181 ccctgaaagg catacattgt cacagattcc tgtgcactga tccctgctct ttttaagact
      241 tttgtcacgg tctttagtat gatcttgata tcggtaagaa atgaaatgtt atccacatat
      301 tcaaggtcga attcaaactt ttcttcccag ctgatggcat ttcttccatt cacctgtgca
      361 agtcctgtca gtcctggtct tacatctgca cgtcgtttct gtcttgcact gtagaaatct
      421 atgtattgtt tcaataatgg tcttggcccg acaaaactta tatgtccaaa taagatgttg
      481 aagaattcag gtaattcatc aaggcttgtg gatcttaaca tctgtccaaa tcttggaaga
      541 cgttctttgt ctggcagcag atcaccattc tcatcgacgg cgtctgtcat acttctgaac
      601 ttgtagagtg taaatatttc gttatgatat cccggtcttt cttgtttgaa gatcacgggg
      661 ctccctaatt tgatccttac taacagtgct gtgacaagca gaatcgggct tgatacaatg
      721 atacagcaca gtgacaatat gatgtcaaaa aatcgtttga tatatttgtg atacatcttt
      781 atatatactc cgtttacatt ttgttcttat cggtccagta cggatcttat gattcctatg
      841 atccgatcca tatcttcttt tgtattctta atatcacttg gaagacataa tccgcgatca
      901 aagatatctt ctgatactgg tttcttatca tcttcgtaat gagaatagaa ttcacaccct
      961 ttaaatactg gttgttgatg taatggtttc cagatcggac ggctttcgat attctctctt
     1021 gttccagagc ttctatgata tctaatggtt ttgtcttact gtcttcatcg atcgtcatac
     1081 atgataacca gaagtttggt tcactgcatg tttcatatgg attcatatgc acttgtttat
     1141 aatccttgaa agcatctttg tatgtctcat agattgcttt tttcttcgca atgtgttcat
     1201 ccagatgcag aagctgacct cttccaactc ccgctgtgat attagacatt cggtagttat
     1261 atccgatctc cttgtgttca taatgtcgga ccggttcttt tgcctgtgtt gccaggaatg
     1321 ttgcatgctt gatgtattct tcattctctg atgcgatcat tccaccgcct gatgtcgtga
     1381 tgatcttatt tccattgaat gaaaatcctg ccatgactcc gaatcttcct gtctggactc
     1441 ctttatatgt tgctcccaga ctttctgcag catcttcgat cagtggaact ttatattctt
     1501 cacagattgc tttgatctcg tctaccttgg ctggtgttcc ataaaggtgt gcaacgatca
     1561 ctgcttttgc ttctttatgt tcttccaatg ctcttttcaa tgcctgtggt gacatattcc
     1621 atgtctctgg ttcagaatcc acgaatacga tcttgccttt ttcgtaggcg actggattca
     1681 ctgttgcagc aaaagtcagc gaaggacata tcacggtatc tccttccccg acttctaata
     1741 atttcaccgc cagatgcagt gctgcggttc ctgcgcttag tgcactgcag tgtgcgattc
     1801 ctgtatacgc ttccatctca cgttcaaagc cattgacatt tggcccaagt ggtgcgatcc
     1861 agttggtgtc aaatgcttcc tgcacaaact tctgttcttc cccatgcatc gttggtgatg
     1921 ccatatatat acgttc
//
LOCUS       DAOMUE010000344         1933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_16757_length_1933_cov_1.914803, whole genome shotgun sequence.
ACCESSION   DAOMUE010000344 DAOMUE010000000
VERSION     DAOMUE010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16757_length_1933_cov_1.914803"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1605
                     /gene="melB"
                     /locus_tag="PLC71_10820"
     CDS             223..1605
                     /gene="melB"
                     /locus_tag="PLC71_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HRM03166.1"
                     /translation="MGTTNGKVPGRVKYTFAFGALGKDLIYGMMATFAMIYFTDVLKV
                     SPGFVGIVFFVAKLWDAFNDLFMGMIVDNTRSRFGKFVPWLVIGTLVNSVVFVVLFTD
                     FKLTGTSLCIFVAVIYVLWGMTYTIMDIPYWSVIPNLTSDPHEREVVSVLPRIFASIG
                     QSLVIAGFGVQIIAALGGGYIGYHRFAMIIAGTFILTIGVAVCNLKVKENNAPSEKIS
                     FKDVFSIIKKNDQLQSAVGLILLYNVGIQFIMGVAVYYFTYVCGNANMLSAFMISASI
                     AEVVGLIIFPEVAKKLSRHTSFLLACILPFIGSALLLVVGFVCPQNIVLTAVAGVIVK
                     TGTGLELGCATVFLSDVVDYGEYKLGTRNESVVFSLQTLIVKFTAALTSLFIGFALER
                     TGYIPNPNAVQSALTLNSIRFLMCAIPAVSMLVAYVVYKKAYHLDDSFMKKVIQTIEE
                     RRANRNAEQY"
     gene            1589..>1933
                     /locus_tag="PLC71_10825"
     CDS             1589..>1933
                     /locus_tag="PLC71_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HRM03167.1"
                     /translation="MPNSIKKLEEAFKEGKYDQLLKDIYVDESVLAYQQERYIKALES
                     FEKIYGEKEVEIYSAPGRSEVGGNHTDHQFGKVLATSINLDAIAIVAKRDDDVIDLKS
                     EGYERIIVSLSSF"
BASE COUNT          645 a          295 c          389 g          604 t
ORIGIN      
        1 aattgagacc cccgcttttg cggcaatgtc tttgattgtt gccatggaga taaaatcctt
       61 tctcataaat attaatttat ctcattataa cagagaaaag cacaaaagta aatttagtaa
      121 aataaaagta aaaaatttag taaaaatata ttgacaattt tactaaaaag ttttactata
      181 caagtatcaa agacagttta ctaaaaacag gaggaaagaa aaatgggaac aactaatgga
      241 aaggttccag gcagagtaaa atatactttt gcatttggag cattgggaaa agatttaatt
      301 tatggaatga tggcgacatt cgctatgatc tactttacgg atgtattgaa agtatcaccg
      361 gggtttgtag gaatcgtttt cttcgtagca aaactttggg atgcatttaa cgatctgttt
      421 atgggaatga tcgtagataa cacaagatca cgctttggaa aattcgtacc atggctagtg
      481 atcggaactt tagtcaattc cgttgtgttt gtcgtattat ttactgactt taaattaaca
      541 ggaacttcac tttgcatctt cgttgcagtg atctatgtat tatggggaat gacttatacg
      601 atcatggata ttccatactg gtctgtaatt ccaaacctta catcagatcc acacgaacga
      661 gaagttgttt ccgttttacc tagaattttc gcaagtattg gacagtcatt agtcatcgca
      721 ggattcggtg ttcagatcat tgcagcatta ggtggaggat atatcggata tcatcgtttt
      781 gctatgatca ttgcaggaac atttattctt acgatcggag ttgcagtctg caacttaaaa
      841 gtaaaagaaa acaatgcacc aagtgagaag atttctttca aagatgtatt ctcaatcatt
      901 aagaaaaatg accagttaca gtcagctgta ggactgattc ttttatataa tgtaggaatt
      961 cagtttatca tgggtgttgc agtttactac tttacatatg tatgtggaaa tgccaacatg
     1021 ttatcagctt ttatgatcag tgcatctatc gcagaagttg ttgggttgat catcttccca
     1081 gaagtagcaa agaaattatc cagacataca tcatttttac tggcatgtat tttaccattt
     1141 attggttcag cattattatt agtcgttgga tttgtatgcc cacagaatat cgtattaaca
     1201 gcagttgcag gagtgatcgt aaagacagga acaggattag aacttggatg tgcgacagta
     1261 ttcttatctg atgtcgttga ctatggtgag tacaaactgg gaacaagaaa tgaatccgtt
     1321 gtattctctt tacagacatt gatcgtaaaa tttacagcgg ctttaacatc acttttcatt
     1381 ggatttgcac tggaacgtac aggatatatt ccaaatccaa acgctgtaca gtcagcactt
     1441 acattgaatt ctatcagatt tttaatgtgt gcgattccag cggtgtccat gcttgtagcc
     1501 tatgtagttt ataaaaaagc atatcatctg gatgacagtt ttatgaagaa agttattcag
     1561 acaatcgaag aaaggagagc aaaccgcaat gccgaacagt attaaaaaat tagaagaagc
     1621 ttttaaagaa gggaaatatg atcagttatt aaaagatatc tatgtagatg aaagtgttct
     1681 tgcatatcag caggaacgct acatcaaagc attagaatcc tttgaaaaga tttatggaga
     1741 aaaagaagta gaaatctata gtgctccagg aagaagtgaa gtaggaggaa accatacaga
     1801 tcatcagttt ggaaaagttc ttgcaacttc cattaacttg gatgcgatcg caatcgttgc
     1861 aaaaagagat gacgatgtga tcgatctgaa atcagaggga tatgaaagaa tcatagttag
     1921 tcttagttct ttt
//
LOCUS       DAOMUE010000345         1932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_16769_length_1932_cov_1.769313, whole genome shotgun sequence.
ACCESSION   DAOMUE010000345 DAOMUE010000000
VERSION     DAOMUE010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16769_length_1932_cov_1.769313"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1162)
                     /locus_tag="PLC71_10830"
     CDS             complement(<1..1162)
                     /locus_tag="PLC71_10830"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRM03168.1"
                     /translation="MARFTLPRDLYFGENALETLKTIKGNKAVLCLGGGSMKRFGFVD
                     KVVEYLKEAGIETKLIEGIEPDPSVETVMKGAKVMQEFEPDLIIAMGGGSPIDAAKAM
                     WVFYEHPETTFDDIKDPFTIPPLRNKATFIAIPSTSGTATEVTAFSVITDYSTGIKYP
                     LADFEITPDVAIVDPSLAETMPQTLTAHTGMDALTHAIEAYVAGLHSPFSDPLAIHAI
                     EMIFDNLKASYEGDMQARGNMHIAQCLAGMAFSNALLGIVHSLAHKTGAVFDTGHIPH
                     GCANAIYLPYVIQYNAKVCEDRFAMIARKLGLPGVTDKALTLSLIKAVEDLRTSLHIV
                     SNLKEFGVKEDEFLEKKHMIAERAVGDACTGSNPRETSVEDMEKILECIYYGK"
     gene            complement(1406..>1932)
                     /gene="queC"
                     /locus_tag="PLC71_10835"
     CDS             complement(1406..>1932)
                     /gene="queC"
                     /locus_tag="PLC71_10835"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRM03169.1"
                     /translation="CAKKICKEHGVEHHILDMGLLNQLAPNSLTRVDMEVDHEAPEEG
                     TPNSFVDGRNMLFLTFAAVFAKQRDIHVLVTGVSQSDFSGYPDCRHVFISSLETTLTL
                     AMDYEFEVITPLMWIDKKQTWEMADKLGVFDIVKNETLTCYNGVMGDGCGECPACKLR
                     RHGLEEYLKVRGHK"
BASE COUNT          505 a          437 c          321 g          669 t
ORIGIN      
        1 cttttccata ataaatgcat tccaatatct tctccatatc ctcaacagat gtttctctcg
       61 gattagatcc cgtacaagca tctccaaccg ctctctctgc gatcatatgc ttcttctcaa
      121 gaaattcgtc ttcttttacg ccaaattcct tcagattaga cacaatatga agactcgttc
      181 tcagatcttc tactgctttg atcagtgata aagtcagtgc tttatcagtc actccaggaa
      241 gtcctaattt tcttgcgatc attgcgaatc tgtcttcaca gactttggca ttgtactgaa
      301 taacatatgg aagatagatt gcattggcgc atccatgagg aatatgtcct gtatcaaaga
      361 ctgctcctgt cttatgtgcc aaggaatgta cgattccaag caacgcgttg gagaatgcca
      421 ttcctgctaa gcactgtgcg atatgcatat tccctcttgc ctgcatatct ccttcatagg
      481 atgctttcag gttatcaaag atcatctcaa tcgcatggat cgctaatgga tctgagaatg
      541 gggaatgtaa tcctgcaaca tatgcttcga tcgcatgggt taaggcatcc attcctgtat
      601 gtgccgttaa tgtctgtggc attgtctctg caagtgatgg atctacgatc gctacatctg
      661 gtgtgatctc gaaatcagcc agtgggtatt tgattcctgt gctgtaatct gtgatcactg
      721 agaatgctgt tacctctgtt gctgttcctg aggttgatgg aattgcgata aaggttgctt
      781 tgttacgaag tggcggaatg gtaaatggat ctttgatatc atcaaaggtt gtctctggat
      841 gttcataaaa tacccacatt gcctttgctg catcgattgg tgatcctcca cccattgcaa
      901 tgatcaggtc tggttcaaat tcctgcatca ctttggcacc tttcataact gtctctacgg
      961 atggatctgg ttcgattcct tcgatcagtt ttgtctcaat tcctgcttcc ttcaaatatt
     1021 caacgacctt gtcaacaaat ccaaaacgtt tcatggaccc gccacccagg cacaatactg
     1081 ctttgtttcc tttgattgtt tttagtgtct ctaatgcgtt ttctccgaag tataaatctc
     1141 ttggtaatgt aaatcttgcc ataactaaca tctcctttac ctggatgacc gaaattgaac
     1201 cttttcttat atatccaaca tttgaatatc cgatttcaat cgattgtttt gctacaaata
     1261 cattgtacat cattgttaaa attttatcaa tacttttctt gcagcttttt gctatttata
     1321 ctttcgacac tttcatcatt catatatata aacaacaaaa aagaaccttg taccatcaca
     1381 agatcctttt ctctttcgta tatttttatt tatgtcctcg gacttttaaa tattcctcta
     1441 atccatgtct tctcaactta catgctgggc attctccgca tccatcaccc atcactccat
     1501 tataacatgt cagagtttca ttttttacaa tatcaaagac gcctaattta tcagccatct
     1561 cccatgtctg tttcttgtca atccacatca gtggtgtgat cacttcaaac tcataatcca
     1621 tggcaagtgt taatgttgtt tctaaagatg agataaagac atggcggcaa tctggatatc
     1681 cactgaaatc actctgggaa acacccgtta ctaacacatg aatatctctt tgttttgcaa
     1741 aaacagctgc aaatgttaag aataacatat ttcttccatc gacaaatgaa tttggtgttc
     1801 cttcttctgg tgcttcatga tctacttcca tatctactct ggtaagagag tttggtgcta
     1861 actgatttaa caatcccata tccagaatat gatgttctac accgtgttct ttacagattt
     1921 tctttgcaca ct
//
LOCUS       DAOMUE010000346         1914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_16967_length_1914_cov_2.329209, whole genome shotgun sequence.
ACCESSION   DAOMUE010000346 DAOMUE010000000
VERSION     DAOMUE010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16967_length_1914_cov_2.329209"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PLC71_10840"
     CDS             complement(<1..689)
                     /locus_tag="PLC71_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRM03170.1"
                     /translation="MFFKKVKKADLEAVSSRNADQERQENKMMRAWISFGVVIILLIL
                     LFFASINIGSLKVGFGELLSGLFVKYNKDVATIYDLRFPRIIISMLAGAAIAVSGVLF
                     QAVLKNPLADPGIIGISSGASFTAVIITAFAPTLYFFTPIAAFAGGVVAFFMVYCLSW
                     KGGLSPMRIILTGVAVNSLFTGLSSALNSMSGGDRTGVAAIVEANITQKTWDDVTTLL
                     PYVVAGLFLAM"
     gene            complement(690..1733)
                     /gene="isdE"
                     /locus_tag="PLC71_10845"
     CDS             complement(690..1733)
                     /gene="isdE"
                     /locus_tag="PLC71_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HRM03171.1"
                     /translation="MNMMRFTTMKTKLRKYPSIFLILLLAISSCFLTGCVNQHPKDSK
                     KSSQGESVETMLEITDPKEKQAVLNAKAKVKALSGNPRIVATSPAVADICDKLDLDLV
                     GVPKSSVSKIPSRYKKVKKVGLAMSPDMEIVSSLNPDWILAPSSLETDLKPKFEELKN
                     TEYAFLNLRSVQGMYRSVQELGEIFGKQQEAEKMTKEFTTFYKSYTKRNKNKKHPKVL
                     VLMGLPGSYVIATENSYVGSLVKLAGGENVYQNTDQEFLTVNTEDMKKKEPDIIVRAA
                     HALPDQVTKMFNEDFETNDIWKHFDAVKNKRVYDLTYEYFGMSANFKYKKALSELEKD
                     FYQNTKGTQEVKE"
     gene            complement(1699..>1914)
                     /locus_tag="PLC71_10850"
     CDS             complement(1699..>1914)
                     /locus_tag="PLC71_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface heme-binding protein Shp"
                     /protein_id="HRM03172.1"
                     /translation="SLSTAPKTTTKKKTSSEKSVGTWILILTVSMTFSGLVLLGVAAT
                     IVYYYRKNWDKWGGDQDEYDEIYNDEN"
BASE COUNT          535 a          448 c          323 g          608 t
ORIGIN      
        1 aacattgcaa ggaataatcc tgcaaccaca tatggaagca atgttgtgac atcatcccat
       61 gtcttctgtg tgatattcgc ctcaacgatt gctgccacac cagttctgtc cccgccggac
      121 atagaattta aggcactgga taacccagta aataagctgt ttactgccac accagttaag
      181 ataattctca tcggacttaa gccacccttc catgacaaac aatacaccat gaaaaatgcg
      241 accacaccac ctgcaaatgc tgcgatcggt gtgaagaaat atagtgttgg tgcaaatgcc
      301 gtaataatga cagcggtaaa acttgccccg cttgagatac cgataattcc cggatctgcc
      361 agtggatttt tcaataccgc ctggaataac acaccagaaa ccgcaatggc cgctcctgct
      421 aacattgaaa tgatgattcg tggaaaacgc agatcataga tcgttgccac gtctttgtta
      481 tatttgacaa acagaccaga aaggagctcc ccaaatccaa ctttcagact tccgatatta
      541 atagaggcaa agaataaaag aattaacaat ataatgacaa ccccaaagga aatccatgct
      601 cgcatcattt tattttcctg cctctcctga tcggcattcc tcgaactaac tgcttcaagg
      661 tctgcttttt ttaccttctt gaaaaacatt tattctttca cctcctgtgt tccttttgta
      721 ttctgataaa aatctttttc taattcactt aatgctttct tatatttgaa gtttgcactc
      781 attccaaagt attcatatgt caggtcataa acccgtttgt tcttcacagc atcaaaatgt
      841 ttccagatat cgttagtttc aaagtcttcg ttaaacattt ttgtcacctg atctggaagt
      901 gcatgtgccg cacggacaat gatatctggt tctttcttct tcatatcctc agtattgact
      961 gttaaaaact cctgatccgt attctggtaa acattttcac caccagcaag tttgaccaga
     1021 cttccaacat aggaattctc tgtcgcaatg acataacttc ctggaagtcc catcagtacg
     1081 agaactttgg gatgtttttt atttttattt cgttttgtat aacttttata aaatgttgta
     1141 aactctttgg tcatcttctc ggcttcctgc tgcttcccaa agatttcccc taattcctga
     1201 accgaacggt acattccctg cacacttctt agatttaaga atgcatactc cgtattcttc
     1261 agttcttcaa actttggttt taagtcagtt tccagagaag aaggagctaa gatccaatca
     1321 ggatttaaag aagatacgat ttccatatcg ggactcatgg caagtccgac tttctttacc
     1381 tttttgtatc ttgatggaat tttggaaaca ctgctctttg gtacaccgac aagatcaaga
     1441 tccagcttgt cacaaatatc tgcaacagcc ggagatgttg cgacgatcct tggattgcct
     1501 gacaatgctt tgactttcgc ctttgcattt aacactgcct gtttttcctt tggatctgtg
     1561 atctcaagca tagtttccac gctctctccc tgactgctct ttttgctgtc ttttggatgc
     1621 tggtttacac aacctgtcag gaaacaagag gaaattgcaa gcaacaggat caaaaaaatg
     1681 ctcggatatt ttcttaattt agttttcatc gttgtaaatc tcatcatatt catcctgatc
     1741 accaccccat ttatcccagt ttttgcggta atagtaaacg atcgtcgctg ccactccaag
     1801 taagaccaat cctgaaaatg tcatagaaac cgttaagatc aaaatccatg ttcccacaga
     1861 cttttcagaa gatgttttct tctttgtggt cgtcttcggt gccgtagata aact
//
LOCUS       DAOMUE010000347         1910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_17010_length_1910_cov_2.304043, whole genome shotgun sequence.
ACCESSION   DAOMUE010000347 DAOMUE010000000
VERSION     DAOMUE010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17010_length_1910_cov_2.304043"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..989
                     /gene="truB"
                     /locus_tag="PLC71_10855"
     CDS             90..989
                     /gene="truB"
                     /locus_tag="PLC71_10855"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRM03173.1"
                     /translation="MYNGLLNIYKEKAFTSHDVVAKLRGILRQKKIGHTGTLDPNAEG
                     VLPVCLGKGTKLCDMLTGTKKQYKVSFVFEKETDTEDIWGTVTKTHEPLKQDAPIKDT
                     ILSFVGEYDQVPPMYSAKKINGKKLYELAREGKIIERKPERIRIYDISDIEISYPEVT
                     MTVTCSKGTYIRSLCRDIGHKLQNGACMTKLVRTRSSGFQLEYAHTLSEIEEAVKTGK
                     IEEWIIPIEKVFLKYQKGTVKKDYDKVLFNGNPLKKEMFYEIDLDTTKKLRVYDESEQ
                     FIGIYYWKKNMFFPDKIFYDNNQ"
     gene            999..>1910
                     /locus_tag="PLC71_10860"
     CDS             999..>1910
                     /locus_tag="PLC71_10860"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRM03174.1"
                     /translation="MEYIHNTEQFQFHNTVVALGKFDGMHKGHQLIFDELIQYKQLGY
                     QAAVFSFDRPPLNMLKHKHMRVIYTTNEKIKLLARRGIDIYIEHPFTDEFSHLSPEDF
                     VIKVLLEKTGMKVLVVGDDCGFGYKRQGNVELLERMSKEYDFKLIVIPKLELEGEIVS
                     STRIRHLLSEGKVEEANRLMEDPFMICGSVVHGNHMGAEVLQMPTANQIPLEDKLLPP
                     NGVYVSRIHYKDETYYGISNVGVKPTIEGKKHMGVETYILDFHKNIYHKEIEVELLCF
                     KRPEMKFDSLESLSQQMHEDAEFARRYA"
BASE COUNT          718 a          265 c          401 g          526 t
ORIGIN      
        1 agcaggatgt tccatgaaag gaacattgaa agacattcaa agtacgatca taagcgagat
       61 cacagaacaa ttataaggta gaggttttta tgtacaacgg attattgaat atatataaag
      121 aaaaagcttt tacatcgcat gatgttgtcg caaaacttcg agggatctta cgtcagaaaa
      181 agattggtca tacaggaact ttggatccaa atgcagaagg agtattgccg gtctgtcttg
      241 gaaaaggaac aaagttatgc gatatgttaa caggaacgaa gaaacagtat aaggtaagct
      301 ttgtgtttga aaaagagaca gacacagaag atatatgggg aactgttacg aaaacacatg
      361 aaccgttaaa acaagatgca ccgatcaaag atacgatctt gtctttcgtt ggagaatatg
      421 atcaggtacc acctatgtat tctgcaaaga aaattaatgg aaagaagctg tatgaacttg
      481 ccagagaagg aaaaattatc gagcgcaaac cagaacggat caggatctat gatatttcag
      541 atattgaaat ctcttatcca gaagttacaa tgacagttac atgcagcaaa ggtacatata
      601 tccgaagtct ttgcagggat attggacata agcttcaaaa tggagcgtgt atgacaaagt
      661 tagtccgtac aagatccagt ggttttcaat tagaatatgc tcatacatta tcagagattg
      721 aggaagcagt gaaaactgga aaaatcgaag aatggataat tccaattgaa aaggtttttc
      781 taaaatatca aaaaggtacc gtaaagaaag actatgataa ggttttattt aacggaaatc
      841 ctttaaagaa agagatgttt tatgaaatag atctcgatac aacaaagaaa cttcgggtat
      901 atgatgaaag tgaacagttt atcggaatat attactggaa aaaaaatatg tttttcccag
      961 ataagatttt ttatgacaac aaccaatgag aggaagccat ggaatacatt cataatacag
     1021 aacaatttca atttcataat acagtggtag ctcttggcaa atttgacgga atgcataaag
     1081 ggcatcagtt gatctttgat gaactgattc agtataagca actgggatat caggcggctg
     1141 tattttcttt tgaccgtcca ccacttaata tgttaaaaca caaacatatg agagtgatct
     1201 acacgaccaa cgaaaagata aaacttcttg caagaagagg aattgatatt tatatcgaac
     1261 atccatttac agatgaattc tcgcatttat caccagaaga ttttgtgatc aaagtacttc
     1321 ttgaaaaaac aggaatgaaa gttttagttg ttggtgatga ttgtgggttt ggttataaac
     1381 gtcaaggaaa cgtagaatta cttgaacgga tgtcaaagga atatgatttt aaattgatcg
     1441 ttattcccaa acttgaatta gaaggagaaa ttgtatcaag cacaagaatc cgtcatttgc
     1501 tatctgaggg taaagtggag gaagctaatc gtctgatgga agatccgttt atgatctgtg
     1561 gatctgtagt tcatggaaat catatgggag cagaagtact tcaaatgcca acagcgaatc
     1621 agattccatt ggaggataag ttattgcctc caaatggagt atatgtttca agaatccatt
     1681 ataaagatga aacgtattac ggaattagta atgttggagt aaagccaacg atcgaaggaa
     1741 agaaacatat gggagttgaa acatatattt tagatttcca taagaatatc tatcataaag
     1801 aaatagaagt ggaattattg tgttttaaaa gaccagaaat gaaatttgat tctctagaat
     1861 ctttaagcca gcagatgcat gaggatgcag aatttgcaag aagatatgca
//
LOCUS       DAOMUE010000348         1904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_17086_length_1904_cov_2.465657, whole genome shotgun sequence.
ACCESSION   DAOMUE010000348 DAOMUE010000000
VERSION     DAOMUE010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17086_length_1904_cov_2.465657"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /gene="argC"
                     /locus_tag="PLC71_10865"
     CDS             <1..868
                     /gene="argC"
                     /locus_tag="PLC71_10865"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRM03175.1"
                     /translation="DDNMDELASKVDVIFTATPQGLCSSLVSEEILSKVKIIDLSADF
                     RIKDVNRYEEWYGIKHQSPEFIDEAVYGLCEINREDIKKARLIANPGCYPTCSTLSIY
                     PMAKEGLIEMNSVIIDAKSGTSGAGRGAKVNNLYCEVNENIKAYGVATHRHTPEIEDQ
                     LSYASGEEVLLNFTPHLIPMNRGILVTAYATLKKDVSYEEVKAIYDSYYKNEKFVRVL
                     DKDVCPETRWVEGSNYVDVNFKIDKRTNRIIMMGAMDNLVKGAAGQAVQNMNLMFGLK
                     ETEGLELVPMFP"
     gene            895..>1904
                     /gene="argJ"
                     /locus_tag="PLC71_10870"
     CDS             895..>1904
                     /gene="argJ"
                     /locus_tag="PLC71_10870"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HRM03176.1"
                     /translation="MKIINAGVTAPKGFKCAGLRAGVKPGKTNKDMAMIVSEVPAKIA
                     GTFTKNIVKAAPVLWGKKIVEEQEAVRAVVINTGIANACTGAQGYENVVTEAKEVAKL
                     LDIKPEEVVVGSTGVIGQQLPMDVIKEGIKKLVPELKADRQSEEDASWAILTTDTKPK
                     EAAVEVEINGKTVTIAGMCKGSGMIHPNMGTMLGFITTDAAIDKALLLELQRELIEDT
                     FNMVSVDGDTSTNDTVFVFANGMAENKEITEKDADYEAFREGLLYVNQSLAKQIAGDG
                     EGCTRLFEVHVAGADSKENAKILSKSVVTSSLTKAAIYGKDANWGRILCALGYAGVDF
                     DPV"
BASE COUNT          695 a          296 c          446 g          467 t
ORIGIN      
        1 ggatgataac atggatgagc ttgcatctaa agtggatgtg atctttacag caacaccgca
       61 gggactttgt agttctttag tatcagaaga aatcttatct aaagttaaga tcattgatct
      121 aagtgcggac ttccgtatta aagatgtaaa tcgatatgaa gaatggtatg ggatcaaaca
      181 tcagagtcca gaatttattg atgaggcagt ttatggactt tgcgagatca atcgtgaaga
      241 tatcaagaaa gcacgactga tcgctaaccc aggatgctat ccaacatgtt ctacattatc
      301 catttatcca atggcgaaag aaggactcat tgagatgaac agtgtgatca tcgatgctaa
      361 atcaggaaca tccggagctg gacgtggtgc gaaagtcaat aacttatatt gtgaagtaaa
      421 tgaaaatatc aaagcatatg gagttgcaac tcacagacat acgccagaga tcgaagatca
      481 gttaagctat gcatctggag aagaagtatt attaaacttc acaccacact taattccaat
      541 gaaccgtgga attttagtga cagcatatgc aacacttaag aaagatgtaa gttacgaaga
      601 agtgaaagca atctacgact cttattataa gaatgaaaaa ttcgtcagag tgttagataa
      661 agatgtctgc cctgagacaa gatgggtaga gggaagtaac tatgtggatg tcaactttaa
      721 gatcgataaa cgtacaaacc gtattatcat gatgggtgcc atggacaacc ttgtaaaagg
      781 tgcagctgga caggccgtac agaacatgaa tcttatgttt ggattaaaag aaactgaagg
      841 attagaatta gtaccaatgt tcccatagag ctttggtaag gaaggactag aattatgaag
      901 atcatcaatg ccggagtaac agctccaaaa ggttttaaat gtgcaggact tcgtgctgga
      961 gtgaaaccag ggaagacaaa caaagacatg gcaatgatcg taagtgaagt gccagcaaag
     1021 atcgcaggaa catttacaaa gaacattgta aaagccgctc cagttttatg gggaaagaag
     1081 atcgtagaag aacaagaagc agtcagagca gtggtaatta atacaggaat tgcaaatgca
     1141 tgtacgggtg cacaaggata tgagaatgtt gtaacagaag caaaagaagt agcgaaattg
     1201 ttagatatta aaccagagga agttgtcgta ggttccacag gagttatcgg acagcagctg
     1261 ccaatggatg tgatcaaaga agggatcaag aagttagttc cagaattaaa agcagacaga
     1321 caaagtgaag aagatgcatc ctgggctatc cttacaaccg atacaaaacc aaaagaagcg
     1381 gcagtagaag tagagatcaa tggaaagaca gttacaattg caggtatgtg caaaggttct
     1441 ggaatgatcc acccaaacat gggaacaatg cttggattta tcactacaga tgcagcaatc
     1501 gataaagctt tattattaga actgcagagg gaactcattg aagatacatt caatatggta
     1561 tcagttgatg gagatacatc tacaaatgat acagtttttg tatttgcaaa tggaatggca
     1621 gaaaataaag aaattacaga aaaagatgca gactatgaag cattcagaga aggattatta
     1681 tatgttaacc agtctcttgc aaaacagatc gcaggggatg gagaaggatg tacacgttta
     1741 tttgaagttc atgtagctgg tgcagactcc aaagaaaatg cgaagatctt aagcaaatct
     1801 gtcgtaacat caagtcttac aaaagcagca atttacggaa aagatgcaaa ctggggaaga
     1861 attctttgtg cacttggtta tgctggagtt gattttgatc cagt
//
LOCUS       DAOMUE010000349         1892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_17226_length_1892_cov_2.713119, whole genome shotgun sequence.
ACCESSION   DAOMUE010000349 DAOMUE010000000
VERSION     DAOMUE010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17226_length_1892_cov_2.713119"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLC71_10875"
     CDS             <1..373
                     /locus_tag="PLC71_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRM03177.1"
                     /translation="TGLYFYDNNVVKYAKELKPSARGELEITDLNKIYLEKGELDVEL
                     LGQGFTWLDTGTHESLVEATNFVKTVETHQHRKIACLEEIAYLNHWITKDQLLTDIEP
                     LKKNQYGQYLMDVMAGKFLDK"
     gene            384..1406
                     /gene="rfbB"
                     /locus_tag="PLC71_10880"
     CDS             384..1406
                     /gene="rfbB"
                     /locus_tag="PLC71_10880"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRM03178.1"
                     /translation="MKILVTGGAGFIGGNFVHHMVNKYPDYEIVNLDLLTYAGNLETL
                     KPVEDKPNYKFVKGDIADREFIMDLFEKEKFDIVVNFAAESHVDRSIEDPSIFVKTNV
                     EGTVVLLDAAKKYGVKRYHQVSTDEVYGDLPLDRPDLFFTETTPLHTSSPYSSSKASA
                     DLFVLAYHRTFGLPVTISRCSNNYGPYHFPEKLIPLMISRALADEELPVYGRGENVRD
                     WLHVSDHCEAIDLIIHKGKVGEVYNVGGHNERTNLEVVKTILKALDKPESLIKFVTDR
                     PGHDMRYAIDPTKLETELGWEPKYNFDTGIAQTIEWYLNNKEWWQNILSGEYANYFDK
                     MYGNRL"
     gene            1415..>1892
                     /locus_tag="PLC71_10885"
     CDS             1415..>1892
                     /locus_tag="PLC71_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRM03179.1"
                     /translation="MRVQKQTKRVLYWGITLVLMILIFSFSAKNGTSSAGMSIGYAKE
                     LARILSFLGFFQIHSNADLMMHAEAVHTVVRKTAHFTEYAVLGFFTYKAVSCDVKDRK
                     KAVLMAQLISTGYASTDEIHQMFVPGREGKVFDVMIDSCGAFCGIMVSCITQKFIEK"
BASE COUNT          674 a          328 c          402 g          488 t
ORIGIN      
        1 cacaggatta tacttctacg ataacaatgt cgtaaaatat gcaaaagaat taaaaccatc
       61 tgccagagga gaattagaga tcacagattt aaataagatc tatctggaaa aaggtgagtt
      121 agatgttgaa ttacttggac agggatttac atggttagat acaggaacac atgaatcctt
      181 agtggaagca acaaactttg taaaaacagt cgaaacacat cagcacagaa agatcgcctg
      241 cttagaagag atcgcatatt taaatcactg gatcacaaag gaccagttat taacagatat
      301 cgaaccatta aagaaaaacc agtatggaca gtatttaatg gacgttatgg caggaaaatt
      361 tttagataaa taaggagaat agcatgaaaa tattagtaac cggtggagcc ggatttatcg
      421 gtggaaactt tgttcatcat atggtaaaca aatatcctga ttacgagatc gtaaacttgg
      481 atttattaac atatgcagga aaccttgaaa cattaaaacc agtggaagac aaaccaaatt
      541 acaaatttgt caaaggtgat atcgcagacc gtgaatttat catggattta tttgaaaaag
      601 aaaaattcga tatcgttgta aacttcgcag cagaaagcca tgtagaccgt tctattgaag
      661 atccatctat ttttgtaaaa acaaacgtag aagggacagt cgtactgtta gatgcagcaa
      721 agaaatacgg tgtaaaacgt taccatcagg tatctacaga tgaagtatac ggagatcttc
      781 cattagatcg cccagattta ttcttcacag agacaacacc actgcacaca tcaagcccat
      841 acagcagttc caaagcaagt gcagacttat ttgtattagc ttaccacaga acatttggac
      901 ttccagtgac gatctcaaga tgctcaaaca actacggacc atatcatttc ccagagaagc
      961 tgattccact aatgatcagc cgtgccttag cagacgaaga acttccagtc tatggaagag
     1021 gtgaaaatgt ccgtgactgg ttacacgtat ctgaccactg cgaagctatt gacctgatca
     1081 tccacaaagg aaaagtcggg gaagtttaca acgtaggtgg acataacgaa cgtactaact
     1141 tagaagttgt aaagacaatc ttaaaagcgt tagacaaacc agaaagcctg atcaaattcg
     1201 taacagaccg tccaggacat gacatgcgtt atgcgatcga cccaacaaaa ctagagacag
     1261 aacttggatg ggaaccaaaa tataactttg acacaggaat tgcacagacg atcgaatggt
     1321 acttaaataa caaagaatgg tggcagaaca ttttatctgg agagtatgca aactatttcg
     1381 ataagatgta cggaaaccgc ttataggagt ctgtatgaga gtacagaaac agacaaaacg
     1441 agtgttatac tgggggataa cactcgtttt gatgattttg attttttcat tttctgcaaa
     1501 gaacggcaca tcctcagcag ggatgagtat cggatatgcg aaagaacttg caaggatcct
     1561 aagctttctt ggattcttcc agattcattc taatgctgat cttatgatgc atgcagaagc
     1621 ggtgcatacg gttgttagaa aaacagcaca ttttacggag tatgcagtac ttggattctt
     1681 tacgtataag gcagtgtctt gtgatgtgaa agatcgaaag aaagcagtgt taatggcaca
     1741 gctgatttct acaggatatg catcaaccga tgagattcat cagatgtttg ttccaggaag
     1801 agaaggaaaa gtatttgatg tgatgatcga tagctgtggg gcattttgtg gaattatggt
     1861 atcttgtatc acgcagaaat tcatagagaa aa
//
LOCUS       DAOMUE010000350         1885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_17292_length_1885_cov_1.798361, whole genome shotgun sequence.
ACCESSION   DAOMUE010000350 DAOMUE010000000
VERSION     DAOMUE010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17292_length_1885_cov_1.798361"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="PLC71_10890"
     CDS             <1..1514
                     /locus_tag="PLC71_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HRM03180.1"
                     /translation="VDVKRILREKIYEKLLCLGASYREHVSTSEVVQMSTEGVEQLEI
                     YFGKYLPQLFYSILAPVTLFIILSFISLKASAVLLICVPLIPISIVAVQKLAKKLLNK
                     YWGLYTTLGDSFLENLQGMTTLKIYQADEMKAVEMDEEAQNFRKITMKVLTMQLNSTS
                     VMDIIAYGGAAVGMITAIHQYIAGNITIGGALIILLLASEFFIPLRLLGSFFHIAMNG
                     MAASDKIFGLLDLEEGESGESKFPQGELEISLDDVHFAYEKDREILKGITMNFPANSF
                     ISFVGESGCGKSTLAGILTARNRGYTGSVKIGGVDISDIMENELMSHMTKITHNSYLF
                     AGTVKENLRMAKPDASKEEMEEVLEKVNLLAFLKEQDGLQTVLSERGENFSGGQRQRL
                     ALARALLFDTPIYIFDEATSNIDMESEEMIMNVIRELSKTKMILFISHRLSNVVDSDC
                     IYMMKDGIVAEEGTHEHLMRKKGAYQHLFDKQKELESYAANMSWNESLKKKVV"
     gene            1516..>1885
                     /locus_tag="PLC71_10895"
     CDS             1516..>1885
                     /locus_tag="PLC71_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM03181.1"
                     /translation="MENTTKQRRSSIKIMGKMIGLVKPLAHIMTAAVLLGTLGYLCAI
                     FLTITAGHGVLLAMKGQRVTKYLIILVVLAVMHGILHYAEQYCNHFIAFKLLAIIRHQ
                     VFAVLRKLCPAKLDGREKGNL"
BASE COUNT          599 a          296 c          450 g          540 t
ORIGIN      
        1 gtgttgatgt gaaaagaatt ttacgagaaa agatctatga gaaattgtta tgtttagggg
       61 cttcttatcg ggaacatgtt tcaacatcag aagtcgtaca gatgagtacc gaaggtgtgg
      121 agcagcttga aatttatttt gggaaatatc ttccgcagtt gttttacagc atcttagcac
      181 cagtaacatt atttatcatt ttatccttta tcagtttaaa ggcaagtgcg gtactgctga
      241 tctgcgttcc attgattccg atttcaatcg ttgctgtgca gaagctcgca aagaagctat
      301 taaataaata ctggggacta tacacaacac taggagacag ttttcttgag aatcttcagg
      361 gaatgacaac attaaagatc tatcaggcgg atgaaatgaa agcggttgag atggatgagg
      421 aagcgcagaa ttttcgtaag atcacgatga aagttttgac aatgcagtta aattcaacca
      481 gcgtcatgga tatcatagcc tacggtggcg ctgctgtcgg aatgatcaca gcgattcatc
      541 agtatattgc tggaaatatc acgattggcg gagcattgat cattttattg ttggcttcag
      601 aattttttat tccgttaaga ttgttaggat catttttcca cattgcgatg aatggtatgg
      661 cagcgagtga taagattttt ggactgcttg atctggaaga aggagagagc ggagaatcca
      721 agtttccaca gggagagtta gagatttcac ttgatgatgt tcattttgct tatgaaaaag
      781 atcgtgaaat cttaaaagga atcacaatga acttccctgc aaacagcttt atttcttttg
      841 ttggggaatc tggctgcgga aaaagtacac tggcagggat cttaacagcg agaaatcgtg
      901 gatatactgg aagtgtcaag atcggtggtg tggatatctc cgatattatg gaaaatgaat
      961 tgatgtccca tatgacgaag atcacacata acagttattt atttgcagga acagtcaaag
     1021 aaaatcttcg tatggcaaaa ccagatgcaa gcaaagaaga gatggaagaa gtgttagaga
     1081 aagtaaatct tctggcattt ttaaaagaac aggatggatt acagactgtc ttgtcagaac
     1141 gcggggaaaa cttctcaggt ggtcagagac agcgtctggc acttgcaaga gcgctgctgt
     1201 ttgatacacc gatctacata tttgacgaag cgacatccaa tattgatatg gaaagtgaag
     1261 aaatgatcat gaatgtcatt cgggaattat caaagacaaa gatgatcttg tttatttccc
     1321 acagattatc caatgttgta gattctgact gcatttatat gatgaaagat ggaattgttg
     1381 cagaagaggg aacacacgaa catttaatgc gtaaaaaagg tgcataccag catttatttg
     1441 ataagcagaa ggaactggaa tcttatgctg caaatatgtc atggaatgag tctttaaaga
     1501 agaaggtggt gtaagatgga aaatacaaca aaacaaagaa gaagttccat aaagattatg
     1561 gggaaaatga tcggtcttgt aaaaccactg gcacacatta tgacagcggc agtgcttctt
     1621 ggaaccttag ggtatctgtg tgccattttc ctgacgatca cagcaggaca tggtgtactt
     1681 cttgctatga aagggcagcg tgtgacgaaa tatctgatta ttttggtggt tcttgcagtg
     1741 atgcatggaa tcctccatta tgcagaacag tattgtaacc attttattgc atttaaactg
     1801 ttagcgatca tcagacatca ggtatttgcg gtacttcgga agctttgccc agctaaatta
     1861 gacggcaggg aaaaggggaa tctga
//
LOCUS       DAOMUE010000351         1882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_17329_length_1882_cov_2.652436, whole genome shotgun sequence.
ACCESSION   DAOMUE010000351 DAOMUE010000000
VERSION     DAOMUE010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17329_length_1882_cov_2.652436"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLC71_10900"
     CDS             complement(<1..450)
                     /locus_tag="PLC71_10900"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRM03182.1"
                     /translation="MSKINKITPLTNVKFLNLYELEAENKFGDTHPYYVASRKNENIM
                     MAATKEVIPDGVVIYSLFGEKADHLIMVRQFRYPLNDYIYELPAGLIDPGESIKETAI
                     REMKEETGLTFTPAEFDDFLSRPHFSSAGMTDETNCTVYGISTGMPNL"
     gene            573..1175
                     /locus_tag="PLC71_10905"
     CDS             573..1175
                     /locus_tag="PLC71_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4362 domain-containing protein"
                     /protein_id="HRM03183.1"
                     /translation="MRKKIMATILGTAMLLTAVSFTGCGRSVKDSTSAKTEKTTKETK
                     RDEKIEIKILSKYDQIKKLLSKYPKEMTAEDTSYQGIVVIEYDSFGKGSKKLWNQFLK
                     NVKDHKDCAIVICQYTVEGDPILQYVSNVNGKFYYVEDSTRDAYGSEKYVQYTYDYYK
                     IYKQDGHYTAILTMDNKLTFDEAQDVRSLKTAIQLLDVKQ"
     gene            1198..1635
                     /locus_tag="PLC71_10910"
     CDS             1198..1635
                     /locus_tag="PLC71_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRM03184.1"
                     /translation="MKISTKGRYAVRLMYDLAMHHTGDWIALKDISRRQEISVKYLEQ
                     IVRQLSIRGYLKSLRGPKGGYQLSRDPKDYTIYEILKITEGSLQPVACLDDKVNQCER
                     YHECPTIEIWEGLGQVIEEYLSGITLEDVVNKARIKGGNDYVI"
BASE COUNT          724 a          255 c          372 g          531 t
ORIGIN      
        1 aagattaggc attccagtag aaattccata aaccgtgcaa ttggtctcat ctgtcattcc
       61 agcagaagag aaatgaggac gacttaagaa atcatcaaat tcagcgggtg taaacgtaag
      121 acctgtttct tctttcattt cacggattgc agtttctttg atagattctc cgggatcgat
      181 caatccggca ggtaattcat atatataatc atttaaagga taacgaaatt gtcttaccat
      241 gatcagatga tcagcttttt ctccaaacag actataaatg acaacgccat caggaatgac
      301 ttctttcgtg gcagccatca ttatattttc attcttgcga ctggcaacat aatatggatg
      361 tgtatctcca aatttatttt cagcttctaa ttcataaaga ttcagaaatt ttacatttgt
      421 tagtggtgtg attttattta tcttactcat agatattacc tccctgtata tttttattgt
      481 aaagcatcac aacaagattg acaatagaca taaaaacaag tattatatac taaaacgata
      541 tgaaataagg ggattacaag aaaggagtta tgatgagaaa gaaaattatg gctacaatac
      601 ttggtacagc gatgctttta acagcagttt cttttacagg ttgtggaaga tcagtcaagg
      661 attctacaag tgcgaagacg gagaaaacca caaaagaaac aaaaagagat gagaagatcg
      721 aaataaaaat actttcaaaa tatgatcaga taaaaaaact actttcaaaa tatccgaaag
      781 aaatgacggc agaagataca tcatatcagg gaattgtggt aattgaatat gattcatttg
      841 gcaaaggaag caaaaagctt tggaatcagt ttttaaagaa tgtaaaagat cataaagatt
      901 gtgctatcgt aatttgtcag tataccgtag agggagatcc gattcttcag tatgtttcta
      961 atgtaaatgg gaagttttac tatgtagagg attctacgag agatgcttat ggcagtgaaa
     1021 aatacgtaca gtatacttat gactactaca agatttataa acaggatggc cattacacag
     1081 caattctgac aatggataac aaactgacat ttgatgaggc acaagatgtc agaagcttaa
     1141 agacagcgat acagttactg gatgttaaac aataattaag aaaatggagc agagaatatg
     1201 aaaatttcaa caaagggaag atatgcagtc cgtttaatgt atgatcttgc aatgcatcat
     1261 acaggcgatt ggatcgcatt aaaagacata tcaagaagac aggagatatc tgtaaaatat
     1321 ttggaacaga tcgtaagaca gttaagtatc aggggatatt taaagagttt gcgaggacct
     1381 aaaggaggtt atcagctttc cagagaccca aaagattata cgatctatga aatcttaaag
     1441 ataacagagg gaagcttaca gccagtagcg tgtttggatg ataaggtgaa tcagtgtgaa
     1501 cgctaccatg aatgtccaac gatagagatc tgggaaggat taggacaggt aattgaagaa
     1561 tatttaagtg gaattacatt agaagatgtc gtaaataaag caagaatcaa aggtggaaac
     1621 gattatgtga tctaaggatc acatggtcgt ttttctttta ttaaaaatat gatgagagtg
     1681 tcaaaaggaa tgcgaaagat aaaagagttt gacattgttt taaaatctga tatactaata
     1741 acatattaaa aagataggaa ttatatgaaa ataaaatcaa aggagtaaaa ataatgtcaa
     1801 atatatataa aaatgttata gaattaatcg gaaaaacacc gcttgtagaa gttgaaaatg
     1861 tagaaaaaag atttgaagcc ta
//
LOCUS       DAOMUE010000352         1858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_17628_length_1858_cov_1.986134, whole genome shotgun sequence.
ACCESSION   DAOMUE010000352 DAOMUE010000000
VERSION     DAOMUE010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17628_length_1858_cov_1.986134"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /gene="folK"
                     /locus_tag="PLC71_10915"
     CDS             <1..227
                     /gene="folK"
                     /locus_tag="PLC71_10915"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRM03185.1"
                     /translation="GRVRTIHWGPRTLDLDILMYDDLVMNERRLTIPHKEMHKRLFVL
                     EPLEEIAPYLMHPLLGQTITQLKEKIKEEQ"
     gene            231..1364
                     /locus_tag="PLC71_10920"
     CDS             231..1364
                     /locus_tag="PLC71_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HRM03186.1"
                     /translation="MRDLTEIRQQIDQIDQKMLALFKERMGCSAEVAEYKRGTGKAIY
                     DPVRERQKIDALTKDEDELIIKKSVEEMFLQMMSISRRYQYSLLSQEDAYIDQTFEEV
                     EALDINEDTKVVYQGIPGAYQEQAMVQYFGEDVKNFSVPEFKDVVKTLDRGEADYGVL
                     PIENTSAGTVSGIYDMILDYDICVVGEESVDVRHVLAAIPGTSLDKIEKVYSHPQGLM
                     QCKGFLDEHPDWDQVKVANTAISAKKVADDNKPVKAAICSERAAKMYGLEILKREVND
                     EGNNTTRFVIMSKKKQYRKDAGKVSISFSVPHESGSLYNILTHFMFNNVSMSNIESRP
                     LPGRKWEYGFYVDVIGNLDDPAIRNSLAGIKEETKDFKILGNF"
     assembly_gap    1543..1552
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1553..>1858
                     /locus_tag="PLC71_10925"
     CDS             <1553..>1858
                     /locus_tag="PLC71_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRM03187.1"
                     /translation="EKVAKGTPEHKLTKIEVTDGDKVVQKFKAVSHTMERLDIMIDRN
                     EQTGRAGSIDLNVKDSKGKSIFHITPTLLEVDGLTDMKATMRRTLRNENKWYKVVVNQ
                     "
BASE COUNT          688 a          257 c          441 g          462 t
ORIGIN      
        1 cgggaagagt cagaacaatt cattggggac caagaacatt agatcttgat attctgatgt
       61 atgatgatct ggtgatgaat gagagaagac tgacgattcc acataaagag atgcacaaga
      121 gattatttgt attagaacca ttagaagaaa tcgccccata tctgatgcat ccgcttcttg
      181 gacagacgat cacgcaatta aaagaaaaga taaaagagga acagtaactt atgagagatt
      241 tgacggagat aaggcagcag atcgatcaga tcgatcagaa aatgcttgca ttatttaaag
      301 aaagaatggg atgttctgca gaagttgcag agtataaaag aggaacagga aaggcaattt
      361 atgatcctgt tcgtgaaaga cagaaaattg atgccttgac aaaagacgaa gatgaactta
      421 tcattaaaaa gtctgtcgaa gagatgtttc ttcagatgat gagtatcagc cgaagatatc
      481 agtatagtct gttatcacaa gaagatgctt atattgatca gacatttgaa gaagtggaag
      541 cactggatat caatgaagat accaaagttg tatatcaggg aattccaggg gcatatcagg
      601 aacaggcaat ggtacagtat tttggagagg atgtcaagaa tttttctgta ccagaattta
      661 aagatgttgt aaagactcta gatcgaggag aagctgatta tggagtattg ccaatagaga
      721 atacttcagc gggaacagtc agtggaattt atgatatgat ccttgactat gacatttgcg
      781 tcgtgggaga agaatctgta gatgtaaggc atgtattggc tgcaattcct ggaacgagct
      841 tagataagat tgaaaaagta tattctcatc cacaaggatt gatgcagtgt aaaggattct
      901 tggatgaaca tccagactgg gatcaggtaa aagttgcaaa tacagcgatc agcgcaaaga
      961 aagtagcaga tgataataag cctgtaaaag ctgcaatctg cagtgaacgt gctgcaaaga
     1021 tgtatggact tgagattttg aaacgtgagg tgaatgatga aggaaataat acaacaagat
     1081 ttgtgatcat gtcaaagaag aaacagtaca gaaaagatgc gggcaaagtc agcatcagtt
     1141 ttagcgtgcc acatgaatca ggatctttat acaatattct gacgcatttc atgtttaaca
     1201 atgtcagtat gagtaacatt gaatctagac cacttccagg aagaaagtgg gaatacggat
     1261 tttatgtgga tgtgatcggg aatctggatg atcctgcgat ccgtaattcc cttgcaggaa
     1321 ttaaagaaga aaccaaagat ttcaaaatct tagggaattt ttaagagaga atgaaaaaga
     1381 gagaaaaagg aaaaataact ggggaaatac aaacgaatgg aaggtaaggt aaaatgacac
     1441 agacaaatgg tgaaacgaaa ttacaaaaat ttcacagcgt catgcaaaaa gttctgaaat
     1501 atggaattgt tttagcattg atcgcattta tcgtattggt ttnnnnnnnn nngaaaaagt
     1561 tgcaaaagga acaccagaac ataagctgac gaagattgaa gtgacagatg gtgataaggt
     1621 tgttcagaaa tttaaagcag tttcacatac gatggaacgg ttggatatca tgatcgaccg
     1681 aaacgaacag acaggaagag ctggaagtat tgatctgaat gtaaaagaca gtaaaggaaa
     1741 atctattttc cacattactc caacactgtt agaggttgac ggactgacag atatgaaagc
     1801 aacgatgcgt cgtactttgc gtaatgaaaa taaatggtac aaagtagtcg tgaatcag
//
LOCUS       DAOMUE010000353         1852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_17707_length_1852_cov_1.591541, whole genome shotgun sequence.
ACCESSION   DAOMUE010000353 DAOMUE010000000
VERSION     DAOMUE010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17707_length_1852_cov_1.591541"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PLC71_10930"
     CDS             complement(<1..1182)
                     /locus_tag="PLC71_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRM03188.1"
                     /translation="MAKTIRMTVAQAIVKFLDNQYVSMDGEETKFVEGFFTIFGHGIA
                     VGLGEALDTNPGNLRVLQGRNEQGMCHVATAYAKQNGRKKIIPCASSVGPGAANMVTA
                     CATATVNNIPLLVFPADTFASRQPDPVLQQLEQSNSLSTTTNDAFKPVCKYWDRVTRP
                     EHIMTALINAMRVLTDPAETGACCIGLCQDVEGESFDFPEYFFEKRVHRITRPVAVEE
                     EIEDIANVIANAKKPLVIVGGGVRYSEAGEEVEKFCEEFKIPFGETQGGKSACKSSHP
                     YCMGGIGVTGTYASNVLAKDADVVIAIGSRMSDFATGSKRQFANPDVKFVSINNSRYH
                     AYKLDSVKAVGDAKVTVKALAEKLRAVNYVSSYNGEIEEAKAVWDKEMKRLAEYKYDE
                     TF"
     gene            complement(1209..>1852)
                     /locus_tag="PLC71_10935"
     CDS             complement(1209..>1852)
                     /locus_tag="PLC71_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRM03189.1"
                     /translation="LSVDDIDEDYIKQSKAILISGTALAESPSREAALKAVMLAKKND
                     TKIIFDIDYREYNWKSEDEISIYYSMVAREADIIMGSREEFDLTEKLIQPGRTDEETA
                     AAWHAYNAKIVVIKHGMKGSTAYTIDGQNFSIKPFPVNARKGFGGGDGYGSAFLYGIY
                     QGWEVIDCLEFGSAEASMMVRSNSCSEELPNPQQVKDFIKETKEKYGEMIARV"
BASE COUNT          460 a          431 c          286 g          675 t
ORIGIN      
        1 aaatgtttca tcatatttgt actcagccag acgtttcatt tctttatccc atacggcctt
       61 agcttcttca atctctccgt tataagatga aacatagttc actgctctta atttttcagc
      121 taatgctttc actgttactt ttgcatctcc aactgctttc acagaatcta atttatatgc
      181 atgatatctg ctgttattga tagatacaaa cttcacatca ggatttgcaa actgtctctt
      241 agatcctgta gcgaaatcgg acattctgct tccgatcgca ataactacat ccgcatcttt
      301 tgcaagaaca ttggatgcat aagtacctgt aacaccgatt cctcccatgc agtatggatg
      361 acttgattta catgcacttt ttcctccctg tgtctcacca aatggaatct tgaattcttc
      421 acaaaatttt tctacttctt ccccagcttc agagtaacga acacctccac caacaataac
      481 aagtggtttc ttcgcatttg cgatcacatt tgcaatgtct tcaatctctt cttccactgc
      541 cactggtctt gtgatcctgt gaactcgttt ctcaaagaaa tattcaggga aatcaaagga
      601 ttcaccttcc acatcctggc aaagaccgat gcagcatgct cctgtttctg ctggatctgt
      661 taaaactctc attgcattga tcagtgctgt cataatatgt tctggtcttg taactcgatc
      721 ccaatattta cacactggct taaatgcatc atttgttgtt gttgataagc tattactctg
      781 ctctaactgc tgaagaactg gatctggctg tcttgatgca aatgtatctg ctggaaatac
      841 taataatgga atattattca ctgtagctgt cgcacaagct gttaccatat tcgcagctcc
      901 aggtcctaca gatgatgcac aaggaatgat tttctttctt ccattctgtt ttgcataagc
      961 tgttgcaaca tgacacattc cctgttcatt tcttccctga agaactctta agtttcctgg
     1021 atttgtatca agtgcttctc ctagtccaac tgcgattcca tgtccgaaga ttgtgaagaa
     1081 tccttctaca aattttgttt cttctccgtc cattgaaaca tactgattat ctaaaaattt
     1141 tacaattgcc tgagcaactg tcattcttat tgtctttgcc atctttctat cctcctgact
     1201 ttttatcctt atactcttgc gatcatttct ccgtattttt cttttgtttc ttttatgaaa
     1261 tcttttacct gctgtgggtt tggaagttct tcggaacaag aattacttct taccatcata
     1321 gatgcttctg ctgatccaaa ctctaagcag tcaataactt cccatccctg gtagattcca
     1381 tataagaagg ctgatccata accatctcct ccaccgaatc cttttcttgc attgactggg
     1441 aatggtttga ttgagaagtt ctgtccatca atcgtgtatg ctgtagatcc tttcatacca
     1501 tgtttgataa caacgatctt cgcattgtat gcatgccatg cagctgctgt ttcttcatct
     1561 gttcttcctg gctggatcag tttctctgtt aagtcaaact cttctctgga tcccatgatg
     1621 atatctgctt ctcttgctac catagaatag taaatagaga tttcatcttc actcttccag
     1681 ttatattctc tataatcaat atcaaaaatg atctttgtat catttttctt tgctaacatc
     1741 actgctttta aagctgcttc tcttgatggg ctttctgcaa gtgctgttcc tgagatcagg
     1801 attgcttttg actgtttgat gtaatcttca tcaatgtcat cgacagataa ct
//
LOCUS       DAOMUE010000354         1838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_17888_length_1838_cov_2.010656, whole genome shotgun sequence.
ACCESSION   DAOMUE010000354 DAOMUE010000000
VERSION     DAOMUE010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17888_length_1838_cov_2.010656"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..699)
                     /gene="thiW"
                     /locus_tag="PLC71_10940"
     CDS             complement(202..699)
                     /gene="thiW"
                     /locus_tag="PLC71_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HRM03190.1"
                     /translation="MKLKKLTISAMLVAVAVVLSSFSIPIGPSRCFPIQHLCNVLAAV
                     FLGPVYGVSMAFCTALIRNLLGTGSLLAFPGSMVGAFVSAMIFKYTRSKIGAYLGEVI
                     GTGILGGMLCYPIASMMMGQKAALFTFVGPFLASTLCGTVIAAVIITALYKSKAVRLI
                     EEYID"
     gene            complement(706..1410)
                     /locus_tag="PLC71_10945"
     CDS             complement(706..1410)
                     /locus_tag="PLC71_10945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRM03191.1"
                     /translation="MKVTIIHGQSHKGSTYHISKILADKIGGEVKEFFLPRDFGEFCI
                     GCTQCFRKSETLCPHYEKINPLTEALDEADVLIFESPVYVYHVTGSMKAFLDHYGYRW
                     MLHRPEESMFHKQAVCISTAAGAGMKSTNKDMADSFFYWGVPKVYKYGVRVMATSYKD
                     IKPKIKAKIEKDTNKMAYQIKKNAGHVKTGIKTKICFYFMRMLHTRGWDEADLAYWSK
                     KGWDREKRPWKNNKGV"
     gene            complement(1412..>1838)
                     /gene="mscL"
                     /locus_tag="PLC71_10950"
     CDS             complement(1412..>1838)
                     /gene="mscL"
                     /locus_tag="PLC71_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRM03192.1"
                     /translation="KKFLEEFKAFALRGNVMDMAVGVLIGGAFSGIVTSLTDNFIQPI
                     IKLVMTGKVYTLEEISGYASAFGSSVVNFLIMAFILFCLLKAMNKVMSFGSKPEEPAQ
                     PTTKICPFCMSEIDIRATRCPHCTSVQPESEADQKQKRV"
BASE COUNT          549 a          414 c          281 g          594 t
ORIGIN      
        1 caaccttcac caagaagctg accatgacca gctttttgcc gcaaaacagc aaactggtca
       61 agaaatatga caaaaaatac ggaagtgaaa atttcaaaat attccttcca atacttcaag
      121 tcgttaagtc tctttccatg aaatatctcc tgtatcttaa ttccttatgc ccgtaactcc
      181 ttcgaacgct cctttaaatg tttagtctat atattcttcg atcaaacgga ccgcttttga
      241 tttatacaat gctgtaatga tcacagctgc gatcacagtt ccacaaagcg tacttgcaag
      301 gaatggccct acaaatgtaa ataaagctgc tttctgtccc atcatcatag atgcgatcgg
      361 atagcaaagc attccaccta aaattcctgt tccaatcact tctccaagat atgcaccaat
      421 cttagatctt gtatatttaa aaatcatagc actgacaaat gccccaacca tacttccagg
      481 gaacgcaagc aggcttcctg ttccgagtag atttctgatc agtgctgtgc aaaatgccat
      541 agaaactcca tatacagggc ctagaaacac tgcagccagc acgttgcata aatgttggat
      601 aggaaagcat ctgctcggtc cgatcgggat tgaaaatgat gataatacga ctgcaactgc
      661 caccaacatg gcagatattg ttaatttttt taatttcatt taatgtcaca ctcctttgtt
      721 gttcttccaa ggtctttttt ctcgatccca gcctttttta ctccaatagg cgagatcggc
      781 ttcatcccaa cctctggtat gaagcattct cataaaataa aagcaaatct ttgtctttat
      841 tcctgttttc acatgtccag cattcttctt aatctgatat gccattttat ttgtatcttt
      901 ttcaattttt gcttttattt ttggttttat gtctttatac gaagttgcca taacacgaac
      961 accgtattta tatacctttg gcactcccca gtaaaaaaag ctgtctgcca tatctttatt
     1021 tgtactcttc attcctgcac cagcagctgt tgatatacat accgcctgtt tgtgaaacat
     1081 tgattcttct ggtctatgca acatccatcg ataaccataa tgatctaaaa acgctttcat
     1141 agagccagtc acatgataaa catacactgg actttcaaaa atcaatacat ctgcttcatc
     1201 aagtgcttct gttaatggat tgatcttttc ataatgtgga cataatgtct cggatttacg
     1261 aaaacattgt gtacatccaa tacagaattc cccaaaatct cttggcagaa aaaactcctt
     1321 gacttccccg ccaatcttgt ctgccagaat tttggaaata tgatatgtag agcctttatg
     1381 actctgtcca tgtataattg ttactttcat tttatactct tttctgtttc tgatctgctt
     1441 cgctctctgg ctgcacagat gtacaatgag gacatctcgt tgcacgaata tcgatctcac
     1501 tcatacaaaa tggacagatt tttgtagttg gctgtgcagg ttcctctggt tttgaaccaa
     1561 atgacatgac cttattcatt gcttttaaaa gacaaaataa gataaacgcc atgatcagaa
     1621 aattcacaac agaagatcca aatgcagatg catatcctga aatttcttct aatgtgtata
     1681 cttttcctgt cataaccaat tttatgattg gctgaataaa attatctgta agtgatgtaa
     1741 caattcctga aaaagcacca ccgatcaata caccgactgc catatccatg acattaccac
     1801 ggagtgcaaa cgccttgaat tcttctaaga attttttc
//
LOCUS       DAOMUE010000355         1826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_18033_length_1826_cov_1.527950, whole genome shotgun sequence.
ACCESSION   DAOMUE010000355 DAOMUE010000000
VERSION     DAOMUE010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18033_length_1826_cov_1.527950"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLC71_10955"
     CDS             <1..410
                     /locus_tag="PLC71_10955"
                     /inference="COORDINATES: protein motif:HMM:NF021196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03193.1"
                     /translation="QNTKTKQWKWDHSKIESNKALKNALDQAYLDRGGFTGKDVYSDH
                     LTAIIDELSKDAEIKRMTKQIRDIEITTRNISAHNLVSITASWVKKYSGYTPEEIYGF
                     LKNYVKKLRWNIKKEDWNSYDAMNEIIIGKIGQ"
     gene            407..1147
                     /gene="cas6"
                     /locus_tag="PLC71_10960"
     CDS             407..1147
                     /gene="cas6"
                     /locus_tag="PLC71_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HRM03194.1"
                     /translation="MITQLEIKLKYQSEEKLNYNISSVMHGILMGHIDRAYGERMHED
                     GLKPFVQEVCNIKEDEFIWRICTMTEEAKIQIIEPLMKMDQFYMKHRELRLKVCERNL
                     KEISYDTLIENYYFEEQPRNITIKFCSPTAFKSQGNYIFMPTVRLIFQSLIHKYDTFS
                     SGTMVGSEETLEHIERYVMITRYHLRSVNFSLEGVRIPAFVGTITIRVKGPQQLVNLI
                     KMLAVFGQYSAVGIKTALGMGRIQIEGV"
     gene            1152..>1826
                     /locus_tag="PLC71_10965"
     CDS             1152..>1826
                     /locus_tag="PLC71_10965"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM03195.1"
                     /translation="MNDNQRKVIIGGLLHDIGKVLYRSDDGRNHSQRGYDFLKNDVDI
                     KDQDILDQIRYHHSQNIKQADLNKDSLAYITYIADNIASGLDRRDKEDGEGGFVRDIA
                     LESIFNILNHNKGNEHYRPAMLGKDKEINFPTTDKIQYDESFYRSIYQKIQECLKEFS
                     YDDKYINSLLEILEATLSFVPSSTSQKQMIDISLYDHVKITAAIGSCIYEYMKENNET
                     DYEKILY"
BASE COUNT          741 a          223 c          369 g          493 t
ORIGIN      
        1 ggcaaaatac gaaaacaaaa caatggaaat gggatcattc aaagatagaa agtaataaag
       61 cattgaaaaa tgctctagat caagcatatt tggatagagg tggatttaca ggaaaagatg
      121 tatattcaga tcatttgaca gcgatcatag atgagttatc caaagatgca gaaataaaga
      181 ggatgacaaa acagattcgt gatatagaaa taacaacacg aaatatctca gcacataatc
      241 tagtatcaat tacagccagc tgggttaaaa aatacagtgg atacacgcca gaagaaattt
      301 acggattttt aaagaattat gtaaagaaac tgcgttggaa cataaaaaaa gaagattgga
      361 attcatatga tgcaatgaat gagataatca taggaaagat agggcaatga tcacacaact
      421 ggaaataaaa ttaaaatatc aatcagaaga aaaacttaat tacaatatat catcggttat
      481 gcatgggatt ttgatgggac atattgacag agcatatggg gaacgaatgc atgaagacgg
      541 gttaaaacca tttgtacagg aagtatgtaa tataaaagaa gatgaattca tatggagaat
      601 ctgtacgatg acagaagaag caaagataca gatcatagag ccattaatga aaatggatca
      661 gttttatatg aaacatagag aattgcgtct aaaggtatgc gaaagaaatt taaaagaaat
      721 ctcctacgat acattgatag aaaactatta ttttgaagag cagccaagga atattacaat
      781 aaaattttgt tcgccaacgg cctttaagag tcaggggaat tatattttta tgcctacggt
      841 tcgtctgata tttcaaagtt tgatacataa atatgatact ttttcatcag gaacaatggt
      901 tggaagtgaa gaaacgttag aacatattga acggtacgtg atgatcacaa gatatcattt
      961 aagaagcgtg aatttttcgt tagaaggagt aagaatacca gcgtttgtcg ggacgatcac
     1021 aatacgagtc aaaggtccac aacaactagt gaatttgatt aagatgttgg cagtattcgg
     1081 acaatattct gcggtaggaa ttaaaacagc attgggtatg ggaaggatac agatagaagg
     1141 agtgtgataa gatgaatgat aatcagcgaa aagttataat cggtggttta ctgcatgata
     1201 ttggcaaagt attatatcga agtgacgatg gcagaaatca tagccagagg ggatatgatt
     1261 ttttgaaaaa tgatgtggat ataaaagatc aagatatctt agatcagata agatatcatc
     1321 acagccaaaa tatcaaacag gcagatctga ataaggattc tttggcttac attacatata
     1381 ttgctgataa tatagcatca ggattagatc gaagagataa agaagatgga gaaggtggct
     1441 ttgttcgaga tatagcgtta gaaagtatct ttaatatatt aaaccataat aaaggaaatg
     1501 aacattacag accggcaatg cttggaaagg ataaagagat caactttcca acaacagata
     1561 agatacaata tgatgaatct ttttatcgta gtatttatca gaagatacaa gaatgtttaa
     1621 aggaattctc atatgatgat aaatacatta actcattgtt agaaatattg gaagcaacct
     1681 tgtcatttgt tccatcttct acatcacaaa aacaaatgat agatatttct ttatatgatc
     1741 atgtaaaaat aacagcagcg atcggaagtt gtatttatga atatatgaaa gaaaataacg
     1801 aaacagatta tgaaaaaata ctttat
//
LOCUS       DAOMUE010000356         1787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_18557_length_1787_cov_2.822748, whole genome shotgun sequence.
ACCESSION   DAOMUE010000356 DAOMUE010000000
VERSION     DAOMUE010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18557_length_1787_cov_2.822748"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..698)
                     /locus_tag="PLC71_10970"
     CDS             complement(99..698)
                     /locus_tag="PLC71_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRM03196.1"
                     /translation="MKKVELNKKKKQDALLNTAYQLFTEKGFQKTSISDIVNEAGVAK
                     GTFYLYFKDKLDIRYKLIAKKSAEVFKHAYQELQKQSIDHFEDQLIFIVDQVIGALNA
                     NPTLLKLISKHLSWGMFKNSLVEPIDDAQRNIYDIYMDLLNNSGHEFDHPEVMIYMII
                     EMVAGSCYNVILTGEPVSVDELKPYLYQSIRLIIRNHIL"
     gene            853..>1787
                     /locus_tag="PLC71_10975"
     CDS             853..>1787
                     /locus_tag="PLC71_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRM03197.1"
                     /translation="MIQFAKKLVHHRKFIVFLAFILLIPSTIGIVKTRINYDILSYLP
                     DSLETVKGQDIMVDQFGAGAYSMIIVEDMNLHDVQKLKQKLEKVNHVDKIIWYDDIAD
                     LSVPKEMLPEKVRKVFFNKNATMMIAMFKETTSSDNTMEAVTQMRKIVGKQCFISGMS
                     GVVTDIKNLVMQEIPIYVTIAAVLSLIVLFATMESFAVAFLFLLSIGMAILYNLGTNI
                     FLSDVSYLTMALTAILQLGVTMDYSIFLLHSYESNKIRFEGDKERAMAHAISNTFKSI
                     TGSSVTTVAGFAALCFMTFALGANLGIVMAKGVIIG"
BASE COUNT          562 a          316 c          337 g          572 t
ORIGIN      
        1 ttgaatatta taaaaagaaa tcatgtgttg gcaaccgtac ggacacatga tttcttttta
       61 taatattcca ccaataattt ccgtcatcaa actacaaatc acaatatatg attccgtatg
      121 atcaaccgaa ttgactgata caaataaggc ttcaattcat caaccgaaac aggctcccca
      181 gtcaaaataa cattatagca agaccctgcg accatctcga tgatcatata gatcataact
      241 tccggatgat caaattcatg accagaatta ttcaaaagat ccatatagat atcataaata
      301 tttctctggg catcatcgat cggttccacc aaggaatttt taaacattcc ccagcttaga
      361 tgctttgaga tcagttttaa cagagtcgga ttagcattta atgctccgat gacctggtca
      421 acgataaaga tcagttggtc ttcaaaatga tcaatggatt gtttttgaag ttcctgatag
      481 gcgtgtttaa atacttctgc agattttttg gcgattaatt tatatcggat gtctaattta
      541 tctttaaaat acaaataaaa agtaccctta gcaacgccag cttcatttac gatatcggag
      601 atggaggtct tctggaatcc cttttctgta aacagttggt atgccgtatt caaaagggca
      661 tcttgtttct ttttcttatt taattcgacc tttttcatag taatttctcc tttcatcttc
      721 tttagtataa cgaaaaaatg accaaaagtc aaaaactttc aagaagttta ttgacttttg
      781 gtcatttttg gtgtatacca ttatatagat taaaaaatga ctattcatca taaatgtaaa
      841 tgggaggatg ctatgataca atttgcaaag aaattagtac atcatcgaaa attcatcgtc
      901 ttcctggctt tcattttatt gattccatca accattggaa tcgtgaagac aaggatcaat
      961 tatgacatct taagttacct tccagactct ttggagacgg tgaaggggca ggatatcatg
     1021 gtagatcagt ttggagccgg agcctattcg atgatcattg tcgaagatat gaatcttcat
     1081 gacgtacaga aattaaaaca gaaactagag aaagtcaatc acgttgataa gatcatctgg
     1141 tatgatgaca tcgctgatct aagcgttccc aaagagatgc ttcctgagaa agtaaggaaa
     1201 gttttcttta ataagaacgc aacaatgatg atcgcaatgt ttaaagaaac gacatcatct
     1261 gataacacga tggaagcagt cacacagatg cgtaagattg ttggaaaaca gtgttttatc
     1321 agtggtatgt caggggtagt taccgatatt aagaacctgg ttatgcagga aattcctatc
     1381 tacgtaacga tcgcggcagt actttctttg atcgtattat ttgccacaat ggaatccttt
     1441 gccgttgcct ttttattcct gttaagtatc ggaatggcaa ttctttataa cctgggaacg
     1501 aatattttct taagcgatgt gtcctatctt acaatggcat tgactgcgat cttgcagtta
     1561 ggagttacga tggattattc gattttcctt ctgcatagtt atgagagtaa taagatcaga
     1621 tttgaaggtg acaaagaacg tgcgatggca catgcgattt cgaatacatt taagtccatc
     1681 acaggaagtt ccgttacaac ggttgctgga ttcgcagcac tgtgctttat gacatttgca
     1741 ttaggagcta acttaggtat cgttatggcg aaaggtgtta ttatcgg
//
LOCUS       DAOMUE010000357         1771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_18785_length_1771_cov_1.682984, whole genome shotgun sequence.
ACCESSION   DAOMUE010000357 DAOMUE010000000
VERSION     DAOMUE010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18785_length_1771_cov_1.682984"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>750
                     /locus_tag="PLC71_10980"
     CDS             <1..>750
                     /locus_tag="PLC71_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRM03198.1"
                     /translation="LIANEELALLCSNKRWTARFFEECGLNAPQVVSSANDYTQGFPA
                     MFAVLDEMDNLEKSIMIHDEQELSFHASKYANYTIRPFLNGKMYEIDVFCNLDGSPVY
                     ITPRAKEEVEGKESARYRVVRDHKIVEEAKKVIKKLRPSGWMTIFMLREEHTDKDYFI
                     RMEPWYHQANTVSIKAGADAPYAALSMMLGEPMEYKEDAADDNVIFTRFEKSVCLNTR
                     EEPIVEIHDFKDLYHLDEEIGSVIFDLDDTLY"
     assembly_gap    751..760
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <761..1311
                     /locus_tag="PLC71_10985"
     CDS             <761..1311
                     /locus_tag="PLC71_10985"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRM03199.1"
                     /translation="EKDYVRSSFRVVERMLPEVNNIFNKLCAALEKGQPPLETVLKDA
                     GMYSDELLLKCREAIRDHKPEISLYEGVKELFFELHTQKRSIGILIDGTPKVQRAKIE
                     ALGLDKMADEILITDELAGHGNVMEFRKPNDLPFLIMRKRLEIPCRNMAFVGDDIEKD
                     FIAPKALGMECYWKKNEDGLYE"
     gene            1304..>1771
                     /locus_tag="PLC71_10990"
     CDS             1304..>1771
                     /locus_tag="PLC71_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HRM03200.1"
                     /translation="MSKEPKIKPIVIVGAGGMGRDTQWLIERINEEEPTYEILGYIDD
                     GIQQGSIIDGYPILGGMKYLEEYDKEQKLAVAFAIGNAKVREKLVLKCLPNPNLWYPN
                     LIDPSVITSQRVHLGQGNIICTSVIMTTNIEIGNFNLICNRSIVGHDDEIGDYN"
BASE COUNT          636 a          299 c          406 g          420 t
ORIGIN      
        1 ctcattgcaa atgaagaatt agctctgctg tgcagcaaca aacgctggac agctcgtttc
       61 tttgaagaat gtggtcttaa cgcaccacag gtcgtaagca gtgccaatga ctatacacaa
      121 ggattcccag caatgtttgc agtattggat gagatggata atctggagaa aagcatcatg
      181 atccatgatg aacaagaatt aagttttcat gcatcaaaat acgcgaatta cacgatacgt
      241 ccatttttaa atgggaagat gtatgagatc gacgtattct gcaatctaga cggaagcccg
      301 gtttacatca caccaagggc aaaggaagaa gtcgagggaa aagaaagtgc aagatacaga
      361 gttgtaagag atcacaagat cgtagaagaa gcaaagaaag tgataaagaa attaaggcca
      421 tccggatgga tgacgatctt tatgcttcgt gaagaacata cagacaaaga ttattttatc
      481 cgaatggaac catggtatca tcaggcaaac accgtatcaa tcaaggcagg agccgacgca
      541 ccatatgcag cactctccat gatgttaggt gaaccaatgg aatacaaaga ggatgcagcc
      601 gatgacaatg ttattttcac aagatttgaa aaaagtgtat gtttaaatac aagagaagaa
      661 ccgattgtag agattcatga ttttaaagat ctgtatcatt tagacgagga aattggttca
      721 gtgatctttg atctggatga cacattatac nnnnnnnnnn cagagaaaga ttatgtacga
      781 agcagtttcc gagtggtaga acgaatgctt ccagaagtga acaatatctt taataagtta
      841 tgcgcagcac tggaaaaagg gcagccgcca ctggaaacgg tattaaaaga tgcaggaatg
      901 tattcggatg aattattatt aaaatgtaga gaagccatca gggaccataa accagagatt
      961 tcattgtatg aaggagtcaa agaattattc tttgaactac acacgcaaaa gcgaagtatc
     1021 gggatcttga tcgatggaac accaaaagta cagcgagcca agatagaagc cctggggtta
     1081 gataaaatgg cagacgagat actgatcaca gatgaacttg caggacatgg aaatgtcatg
     1141 gaattcagga aaccaaatga tcttccattt ctgatcatga gaaaacgatt agaaatacca
     1201 tgcaggaaca tggcatttgt cggagatgat atcgagaaag attttattgc accgaaagca
     1261 ttaggaatgg aatgctattg gaagaagaat gaggatgggt tatatgagta aagaaccaaa
     1321 gataaaacca atcgtgatcg ttggagccgg tggtatggga agagatactc agtggctgat
     1381 cgaacggatc aacgaagaag aaccaacata tgagattctt ggatacatag acgatggaat
     1441 ccagcaaggc agcatcatag atggttatcc gattcttgga ggaatgaaat acctcgaaga
     1501 atacgacaaa gaacaaaaac ttgccgtcgc cttcgctata ggcaatgcaa aagtccgtga
     1561 aaaactcgtt ttaaaatgtc tgccaaatcc aaacttatgg tatcctaatc tgatcgatcc
     1621 atccgtgatc acatcacaaa gagtgcatct aggacaagga aatatcattt gtacatccgt
     1681 tattatgaca acgaatatag agatcggtaa ttttaacctg atctgcaacc gaagtattgt
     1741 cggacatgat gatgagatcg gagattataa t
//
LOCUS       DAOMUE010000358         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_18862_length_1765_cov_2.551462, whole genome shotgun sequence.
ACCESSION   DAOMUE010000358 DAOMUE010000000
VERSION     DAOMUE010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18862_length_1765_cov_2.551462"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1536
                     /locus_tag="PLC71_10995"
     CDS             52..1536
                     /locus_tag="PLC71_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HRM03201.1"
                     /translation="MNNMPKTKIGIVAVSRDCFPESLSVTRREALIKAYKEKYDPEDI
                     YECPVCIVESEIHMVQALEDIKKAGCDALVVYLGNFGPEISETLLAKHFDGPKMFVAA
                     AEESGDNLLDGRGDAYCGMLNASYNLKLRNIKAYIPEYPVGTAEECADMIHEFLPIAR
                     TVKALSELKIISFGPRPLNFLACNAPIKPLYDLGIEIEENSELDLFEAFNQHEGDERI
                     PAIIKEMEEELGAGNKKPEILSKLAQYELTLKDWIRDHKGYRKYVTIAGKCWPAFQTQ
                     FGFVPCYVNSRLAAQGIPVSCEVDIYGALSEFIGTVVSQDAVTLLDINNSVPADMYNE
                     EIKDNYDYTQKDTFMGFHCGNTAKSKLSFCEMKYQKIMARTLPEEVTQGTLEGDIAPG
                     DITFFRLQSTADTKLRAYVAQGEILPVATRSFGSIGVFAIPEMGRFYRHVLIEKNFPH
                     HGAVAFGHFGKTLYEVFKYIGVDVDEIGFNQPKNMLYKTENPFS"
BASE COUNT          641 a          266 c          370 g          488 t
ORIGIN      
        1 atgaaaagtg gatagttata tttggttgat gtattaaagg aggaaaacaa tatgaataat
       61 atgccaaaaa caaagatcgg gatcgtagca gtcagtcgtg actgcttccc agaaagttta
      121 tcagttacaa gaagagaagc attgataaaa gcatataaag aaaaatatga tcctgaggac
      181 atttatgaat gtccagtctg tatcgtggag agtgagattc acatggtaca ggcattagaa
      241 gatattaaaa aagcaggatg tgatgcatta gttgtttatc ttggaaactt tggaccagaa
      301 atctcagaaa cacttcttgc aaaacacttt gatggaccaa agatgtttgt ggcagcagct
      361 gaggaaagtg gtgataacct cttagatgga agaggagatg catattgtgg aatgttaaat
      421 gcaagttata atctgaaatt aagaaatata aaagcatata ttcctgaata tccagtagga
      481 acagcagaag aatgtgcaga tatgatccat gaatttttgc caatcgcaag aacagtaaaa
      541 gcattaagtg aattgaagat cataagtttt ggaccaagac cactgaactt ccttgcatgt
      601 aatgcaccga tcaaaccatt atatgatctt ggaatcgaaa ttgaagaaaa ctcagaatta
      661 gatttatttg aagcatttaa tcagcatgaa ggggatgaaa gaattcctgc aatcataaaa
      721 gaaatggaag aagaactggg tgcaggaaat aaaaaaccag agattctttc caagcttgca
      781 cagtatgaac tgacattaaa agactggatc agggatcata aaggttacag aaaatatgta
      841 acaattgccg gaaaatgctg gccagcattc cagactcagt ttggatttgt accatgttat
      901 gtaaacagtc gtttggcagc acagggaatt ccagtatcct gtgaagtaga tatctatggt
      961 gcattaagcg aatttattgg aacggttgta agtcaggatg ctgtgacatt attagatatc
     1021 aacaactccg taccagcaga tatgtataat gaagaaatta aagataacta tgactataca
     1081 caaaaagata catttatggg attccattgt ggaaacacag caaaatctaa attatctttc
     1141 tgtgagatga aatatcagaa gatcatggca agaacattac cggaagaagt gacacaggga
     1201 acattagaag gtgatatcgc gccaggagat attacattct tccgtttaca aagtacagca
     1261 gatacaaaat taagagcgta cgttgcacaa ggagaaatcc ttccagtagc aacaagatct
     1321 ttcggatcta ttggtgtatt tgctatccca gaaatgggaa gattttaccg ccatgtactg
     1381 atcgaaaaga atttcccaca ccatggagct gttgcttttg gtcattttgg aaaaacttta
     1441 tatgaagtat tcaaatacat tggtgttgat gtcgatgaga ttggatttaa tcagccaaag
     1501 aatatgcttt ataagactga gaatccattt tcataaataa attttaccta ttaacttttt
     1561 actccttctt gggaaaaact gcatgctgac cgcatgcagt tttttctatg cttagaaaaa
     1621 caagtaaaaa tattactaaa tattatgact gtttgcgcgg agcagcacaa gagttacgaa
     1681 caataagatt gcatggaagt gtcagacgaa tcggagtttg ctgattcaga gaatcctctt
     1741 ttgtccgaag taaaatataa tgagc
//
LOCUS       DAOMUE010000359         1765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_18865_length_1765_cov_2.045614, whole genome shotgun sequence.
ACCESSION   DAOMUE010000359 DAOMUE010000000
VERSION     DAOMUE010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18865_length_1765_cov_2.045614"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PLC71_11000"
     CDS             complement(<1..653)
                     /locus_tag="PLC71_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRM03202.1"
                     /translation="MEFKTPTIEDKEEIDRCILADDTRSCDYATANIILWSPFYQVKY
                     AIIDGLFVSHTDEEGGSFCYPLGKGNKKKVLQYFMDDCKEKKIPFVLHGVTHEMEEEF
                     REIFGDIYEIEYDRDISEYIYDREKLATLSGKKLHGKRNHINRFKENHEWSYEPLSDE
                     NQLEVLAMLMEWKMQNCDPEDIEKHDEICVSKNCVINYKELGLVGGVLRAEGKVVGFS
                     "
     gene            complement(692..1102)
                     /locus_tag="PLC71_11005"
     CDS             complement(692..1102)
                     /locus_tag="PLC71_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRM03203.1"
                     /translation="MMKFEIYILQKKSQDPKMYQEAMGEYLKRLGAYAKVSVVYKKNA
                     KQVEKILKEPGEHYHILPGRDSMTSPGLAQLINESSVQGISKFHFYIGDLGSEEAKPF
                     HVSSFTQSGSLAGTIILEQIYRAYRILNHQPYHK"
     gene            complement(1108..>1765)
                     /locus_tag="PLC71_11010"
     CDS             complement(1108..>1765)
                     /locus_tag="PLC71_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03204.1"
                     /translation="KSTIVVIITSWILFFIAAFLAYAKLDQMKQERLTNEKIFQQTTQ
                     KNTSQGVIRETNDAAETKKEDSETEKKAVEDFMKTYYERVQKNDLENLKNMVEDTNEL
                     TEDQKAIHQYVKKYKNLTYLIKQGADDESYIVYVTYEMKIRKIKTLAPGMTSYYVMKK
                     GDTFCIYNNQKHDTDEITDAKKESQNSKEIKKLTKQINKRYELALKQDKKLKQFFEGN
                     "
BASE COUNT          471 a          368 c          226 g          700 t
ORIGIN      
        1 gaaaatccta cgacctttcc ttccgcacgc agaacacctc cgacaagtcc taattcttta
       61 taattgatca cacaattttt agagacacag atctcgtcat gtttctctat atcttctgga
      121 tcacaatttt gcatcttcca ttccatcagc attgccagta cttctaactg attttcatcg
      181 gaaagtggtt cataggacca ttcatgattt tctttaaaac gattgatgtg atttctcttt
      241 ccatgaagtt tctttcctga taatgttgcg agtttttctc tgtcataaat atattctgag
      301 atatctctgt catattcaat ctcataaata tctccaaata tttcacgaaa ctcttcttcc
      361 atctcatgag ttacaccatg taatacaaac ggaatctttt tttctttgca atcatccata
      421 aaatattgca atactttctt tttatttcct tttccaagtg gataacaaaa agaacctccc
      481 tcctcatcag tatgagatac aaataatcca tctatgatcg catactttac ttgataaaaa
      541 ggactccata aaataatatt cgctgttgca taatcacaac ttcttgtatc atctgctaag
      601 atacatcgat caatttcttc cttatcttca attgttggtg ttttaaactc catctttatc
      661 aactccattc tagtgttttc tcgtaatatt tttatttgtg atatggctga tgattcaaaa
      721 tccgataagc acgataaatc tgttctaata tgatcgtgcc tgccagcgaa ccactttgtg
      781 taaatgacga tacatgaaat ggctttgctt cctctgatcc taaatctcca atataaaaat
      841 gaaactttga aattccctgt acagaacttt cattaatcag ctgtgcaagc cccggacttg
      901 tcatactgtc tcttccaggc aggatatgat aatgttctcc cggttctttt aatattttct
      961 ctacttgttt tgcattcttc ttatatacta cagaaacttt cgcgtatgca ccaagacgtt
     1021 ttagatactc acccattgct tcctgataca tcttaggatc ctgtgatttc ttctgcagaa
     1081 tataaatttc aaatttcatc atatctttta atttccttca aaaaattgtt ttaatttttt
     1141 atcttgtttt aaagccagct catatctttt gttgatctgt tttgtaagtt ttttaatttc
     1201 tttgctattc tggctttctt tttttgcatc tgtgatctca tccgtatcat gtttttgatt
     1261 attatagata cagaatgtat cccctttctt catcacataa tacgatgtca tccccggtgc
     1321 aagtgtttta atcttgcgga tcttcatctc atatgttaca taaacgatat aggattcatc
     1381 gtctgctcct tgtttgatca gatatgtaag attcttatat ttcttcacat actgatggat
     1441 tgccttttga tcctcagtta attcattcgt atcttctacc atattcttaa gattttccag
     1501 atcatttttc tgaactctct cataatatgt tttcataaaa tcctcaactg cttttttctc
     1561 agtctctgaa tcttccttct tcgtttccgc tgcatcattt gtttctctga tcacgccttg
     1621 tgaggtattc ttttgtgttg tttgttgaaa aatcttttca tttgtaagtc gttcttgttt
     1681 catctggtcc aatttcgcat atgccaaaaa agctgcaata aaaaataaga tccagcttgt
     1741 aatgataaca acaattgtag atttt
//
LOCUS       DAOMUE010000360         1762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_18903_length_1762_cov_2.707088, whole genome shotgun sequence.
ACCESSION   DAOMUE010000360 DAOMUE010000000
VERSION     DAOMUE010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18903_length_1762_cov_2.707088"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="PLC71_11015"
     CDS             complement(<1..512)
                     /locus_tag="PLC71_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRM03205.1"
                     /translation="MRNIIRKLAVCFLAWILVCTTVVTGHGMHTAKAAQTFKIHFIDV
                     GCADAALLQYGEGTEAKYALIDTGANQYHNTKDTTIDTTKTPVYEYLNKMGVKHLEFI
                     LLTHPHQDHIGGMEKILSDTTIKIDKIYGNDLNIQYLKSSEDKSKQSSDETNWTEFDT
                     KIYKNFKAAL"
     gene            complement(718..>1762)
                     /locus_tag="PLC71_11020"
     CDS             complement(718..>1762)
                     /locus_tag="PLC71_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRM03206.1"
                     /translation="MHGNPLLSDWVTKKLGTDTWATDLSLMSGLKKYVDDPKAQKEFM
                     DIKLQNKKRLAKYILEHNGVEVDPTSIFDVQVKRLHEYKRQLMNILHVMYLYNQLKKN
                     PNMKFYPRTFIFGAKAAAGYLRAKQTIKLINSVADKVNNDASIKGKLKVVFIEDYRVS
                     NAEWIFAAADVSEQISTASKEASGTGNMKFMLNGAPTLGTMDGANVEIVDEVGAENAF
                     IFGMSSEEVINYENNGGYHPYEIYQNDKDIHEVLDQLVDGTYANGDPELYKDLYQSLL
                     FGDTGSQADMYFILKDFRSYAEAQKKVEEAYRDTKGWAKMAMTNTAGCGKFSSDRTIQ
                     EYVDDIWHLDKIR"
BASE COUNT          463 a          373 c          260 g          666 t
ORIGIN      
        1 tctaaagctg ctttaaaatt cttgtatatt ttggtatcaa attctgtcca atttgtttca
       61 tctgaagact gcttgctctt atcctcgctg cttttcaagt actgaatatt cagatcattc
      121 ccataaatct tatcgatctt gatcgttgta tcagaaagaa tcttctccat acctccaata
      181 tgatcctgat gaggatgtgt cagaagtata aattccagat gttttactcc catcttattt
      241 aaatattcat agactggtgt ctttgttgta tcaatcgtcg tatcctttgt attatgatac
      301 tggtttgctc ctgtatcgat caatgcatac tttgcttctg ttccttctcc atactgtaat
      361 aatgcagcat ctgcacatcc gacatcaata aaatgaatct taaatgtctg tgctgctttt
      421 gccgtatgca ttccatgccc agttacaact gttgtgcaga ccaatatcca tgccagaaag
      481 cacactgcaa gtttccttat aatgttcctc atttgttact ccctttcttt gtttttcatc
      541 tttactcttg atatttcttt tttacaatcg aattttaaca tattcttaat atttttgcaa
      601 tttttttgaa atattttatc atctgttttt acatgaataa aggaagagtt cctcctttgg
      661 gaactcttcc tcttaactta tcatattcaa tactatattt caagactcta tataagacta
      721 tctgatctta tctaagtgcc agatatcatc aacatattcc tgaattgtac ggtcagatga
      781 gaacttgcca catcctgctg tgttggtcat cgccatctta gcccatcctt ttgtatctct
      841 atatgcttct tctactttct tctgagcttc tgcatatgaa cggaagtctt ttaagatgaa
      901 gtacatatct gcctggcttc ctgtatcacc aaataacaga ctctggtaca gatctttgta
      961 aagttctgga tcaccatttg catatgttcc atctactaac tgatctaaga cttcatggat
     1021 gtctttatca ttctggtaaa tctcatatgg atgatatcca ccgttattct cataattgat
     1081 aacttcttct gagctcatac cgaagataaa tgcattctca gctccaactt catcaacgat
     1141 ctctacattc gctccatcca ttgttccaag tgttggtgca ccatttaaca tgaacttcat
     1201 gttacctgtt cctgatgctt ctttggatgc tgttgaaatc tgttcgctga catctgctgc
     1261 cgcaaagatc cattctgcat tggatacacg ataatcttca ataaatacaa cctttaactt
     1321 acctttgatg gacgcatcat tatttacttt atcagctaca gagttgatca gtttgatcgt
     1381 ctgttttgca cgaaggtatc cagctgctgc cttagcacca aaaataaatg ttcttggata
     1441 gaacttcata tttggattct tctttaactg attatacaga tacatcacat gtaaaatatt
     1501 catcagctga cgtttgtatt catgaagtcg ttttacctgt acatcaaaga tggatgttgg
     1561 atcaacttct acgccattgt gttctaagat atattttgca agacgttttt tattctgcag
     1621 tttgatatcc ataaattctt tctgagcttt tggatcatcc acatatttct tcagtcctga
     1681 cattaaagaa aggtctgttg cccatgtgtc tgttccaagt ttcttagtta cccagtcaga
     1741 aagtaatgga ttaccatgca tt
//
LOCUS       DAOMUE010000361         1760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_18934_length_1760_cov_2.586510, whole genome shotgun sequence.
ACCESSION   DAOMUE010000361 DAOMUE010000000
VERSION     DAOMUE010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18934_length_1760_cov_2.586510"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1483
                     /locus_tag="PLC71_11025"
     CDS             86..1483
                     /locus_tag="PLC71_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HRM03207.1"
                     /translation="MKKLKIAGIILVLFILYVIIGMLVPFVHMQSVSKTNKSKIHTET
                     FYSTSNENGSDRAKIVSDNQEALDLRLDMIRKAKKEIILSTFDIREGSSSDDIFSELL
                     KASRRGVKIKILVDGLYGTIHMTGKDIFAAVGSEPNVEIRFYNTPNLLKPWTINGCLH
                     DKYIVADHKYLLMGGRNMFDYFLGTHKGKSKGIDREILIVNQGSKDQGAVGQIRRYFQ
                     KVWNLEVCKTKFSTCSKNKKEKEVKRLLSHAEKVKVPDYDYQKITVPTKKTTLVTNPT
                     TIYGKEPVVFETLKQLMLQAKSKVIIHTPYAVFSKEMYEGIAQVKQQVPNTTMILNSI
                     ASGDNICASADYQKNRSKILGTGVSLYEYMGRHSTHGKSLIIDDDIAVVGSYNFDCRS
                     TYVDTETMLVVQGKEITKQLEEDFSRLKSGSLKVNQDGSYQNDKDVAERTMDSKKQGM
                     IKILSYIIQAFRYLA"
     gene            1517..>1760
                     /locus_tag="PLC71_11030"
     CDS             1517..>1760
                     /locus_tag="PLC71_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HRM03208.1"
                     /translation="MKDIKIIFFDIDGTLIAMDQDTISDKTLEALKRLQEKGVKLCLA
                     TGRGPMLVPHFEGVEFDAFLTYNGSYCYDHSGTLYSN"
BASE COUNT          668 a          262 c          359 g          471 t
ORIGIN      
        1 atcaatagtt taaaatcttt attaaagatg ttaaaataaa gacagtaaaa ttatttatct
       61 aaattaaaat aaggatttta gaacaatgaa aaaattaaaa atagcaggaa tcattctggt
      121 tttgtttatt ttgtatgtga tcattggaat gcttgtacca tttgtacata tgcaatcggt
      181 aagcaaaaca aataagtcaa agattcatac ggaaacgttt tattcaacat caaacgaaaa
      241 tggatcagac cgagcaaaga tcgtcagtga taatcaggaa gcacttgatc tgcggttgga
      301 tatgatacga aaagcaaaga aagagatcat tctatcaacc tttgatatcc gtgaaggaag
      361 cagcagtgat gatatttttt cagaattact caaagcatcc agacgaggcg tcaagataaa
      421 gattctggtt gatgggcttt atggaacaat ccatatgaca ggaaaagata tttttgcggc
      481 agttggaagt gagccaaatg ttgaaattcg tttttataac acgccaaatc ttttgaaacc
      541 atggacgatc aatggatgtc tgcatgacaa atatattgtg gcagatcata agtatctctt
      601 aatgggtggc agaaatatgt ttgattattt ccttggaaca cacaaaggaa aaagcaaagg
      661 tatagacagg gagattctga tcgtcaatca aggatcaaaa gatcaaggtg cagtcggaca
      721 gatcagaaga tattttcaaa aagtctggaa cttagaagtc tgcaaaacaa aatttagtac
      781 atgctcaaag aataaaaaag agaaggaagt caagcgcctt ttatcccatg cagagaaagt
      841 gaaagttcca gattatgatt atcaaaagat tacagttcca acgaagaaaa caacactggt
      901 aaccaatccg acaacaattt atggaaaaga accagttgta tttgaaacgc tgaagcagtt
      961 gatgttacag gcaaaaagca aagttatcat tcatacacca tatgcagtgt tttccaaaga
     1021 aatgtacgaa gggatcgcac aggtgaaaca acaggttccg aacacaacaa tgatcttgaa
     1081 ttcaattgca tcaggagata atatttgtgc atcagcagat tatcaaaaga accgatcaaa
     1141 gattttaggt acaggagtgt cactgtatga atatatgggc agacattcca cacacggaaa
     1201 atctctgatc attgatgatg atatagcagt ggttggttct tacaactttg attgcagaag
     1261 tacctatgta gacacagaaa cgatgcttgt tgtacaagga aaagagatca caaaacagct
     1321 ggaagaagat tttagcagat taaaaagcgg atctttgaaa gtgaatcaag atggaagtta
     1381 tcaaaatgac aaggatgtag cagaacgcac gatggatagc aaaaagcagg gtatgatcaa
     1441 gattttgtct tatatcatac aggcatttcg ttatttagcg taaactcaca tcataatata
     1501 gaaggaaaag aaagacatga aagatattaa aatcatattt tttgacattg atggtacatt
     1561 gatcgctatg gatcaagata cgatttcaga caaaacatta gaggcattaa aacgactaca
     1621 ggaaaaagga gtcaaacttt gtttggcaac aggaagagga ccgatgcttg tcccacattt
     1681 tgaaggagtt gagtttgatg catttcttac atacaatggg tcttattgct atgatcacag
     1741 tggaacactc tatagcaatt
//
LOCUS       DAOMUE010000362         1746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_19144_length_1746_cov_1.715553, whole genome shotgun sequence.
ACCESSION   DAOMUE010000362 DAOMUE010000000
VERSION     DAOMUE010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19144_length_1746_cov_1.715553"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="PLC71_11035"
     CDS             complement(<1..153)
                     /locus_tag="PLC71_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM03209.1"
                     /translation="MYVEMRNIYKQYGNFRASDNVSFGIEKGKLAALLGPSGSGKTTL
                     LRMIAGL"
     gene            complement(155..1027)
                     /locus_tag="PLC71_11040"
     CDS             complement(155..1027)
                     /locus_tag="PLC71_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HRM03210.1"
                     /translation="MNEQQKFEKRKQRTKIILITISVLFMIVMLVLPLFSVITNSLSE
                     GFKFYVSSISTEYVRSALFVTILATLVAVTINTFFGIMAAFLLTKFSFKGKQVLATLI
                     DIPFSISPVIVGLAFLMTFGRLGWTYPAIRAINIFFGTNIRIAFAIPGVILATIFVTF
                     PFVSREIIPILNSQGKDEEEAAALMGASGFTIFRKITLPQMKWGLIYGIILCSARALG
                     EFGAVNALSKTRGETFTLPLEIDALYMSGTTSSITAAFAVSSVLVLIAVVVLILRNIA
                     WYRSQDKQQGKDGR"
     gene            complement(1038..>1746)
                     /gene="cysT"
                     /locus_tag="PLC71_11045"
     CDS             complement(1038..>1746)
                     /gene="cysT"
                     /locus_tag="PLC71_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HRM03211.1"
                     /translation="PNEFFTLISKETVRNAFITSIGSSFIAALINVVFGLIIAWVLVK
                     YEFPGKRLMDGMIELPFALPTAVAGITLSKMYAEDGMIGRYFAHFGIKISYTRIGLLI
                     ALVFIGIPFIVRAVQPVLEKMDDQYEEAAYMLGATPTRTFFKVILPELRPALLTGFGL
                     AFARGIGEYGSVIYISGNSAKEHTQMISYVIMQKLNYIDYKSATAIALVMLIISFVML
                     FVINIIQVRQARRVNAQ"
BASE COUNT          508 a          391 c          300 g          547 t
ORIGIN      
        1 aagtcctgcg atcattcgaa gcaatgttgt ctttccactt ccacttggtc caagcaatgc
       61 tgcaagtttt cctttttcaa tcccaaaact tacattgtct gacgcccgga agtttccgta
      121 ttgtttatag atatttctca tttcgacata cattttatct tccgtccttt ccctgttgtt
      181 tgtcctgtga gcggtaccat gcaatatttc ttaagatcag tacaacgacc gcgatcaata
      241 ctaaaaccga tgatactgca aaggctgctg tgatcgatga tgttgttcct gacatatata
      301 aggcatcgat ctccaatggt aaggtaaatg tctcacctct tgttttggaa agtgcattaa
      361 ctgcaccaaa ttctcctaat gccctggcac tacataagat gatcccatag atcaatcccc
      421 atttcatctg aggaagtgtg atctttcgaa agatcgtaaa tccactggct cccatcaatg
      481 ctgctgcctc ttcttcgtct tttccctgtg agtttaagat cggtatgatc tcccttgaca
      541 caaatgggaa ggtcacgaaa atggttgcta aaatgactcc tggaatcgca aatgcgatcc
      601 ggatattcgt tccaaagaag atgttgatcg ctcggatcgc tggatacgtc catccaagtc
      661 ttccaaatgt catcaagaat gcaaggccta cgataactgg tgaaatggaa aatggaatat
      721 cgatcaaagt tgctaaaacc tgttttcctt tgaaactgaa tttagtcaat aaaaatgctg
      781 ccatgatccc gaaaaatgta ttgatcgtta ccgcaacaag cgttgccaga atcgttacaa
      841 ataaagcgct tcttacatac tccgttgata tggaacttac ataaaattta aatccctcac
      901 ttaatgaatt tgtaataacg gaaaataacg gtaagaccaa catgacgatc ataaataaaa
      961 cactgatcgt gatcaggatg atcttcgttc gctgttttct tttttcaaac ttctgctgtt
     1021 cattcatgat cttctcctta ctgtgcattg actcttcttg cctgtcttac ctgtatgata
     1081 ttgatcacaa ataacatcac aaaggaaatg atcagcatga caagagcaat agctgttgca
     1141 cttttatagt cgatatagtt taatttctgc atgatcacat aagagatcat ctgtgtatgt
     1201 tccttggcac tgtttcctga gatatagatc acacttccgt attccccgat tccccttgca
     1261 aatgccagtc caaatcctgt taataatgca ggtcttaatt ctggtaaaat gactttaaag
     1321 aatgttcttg tcggtgttgc acctaacata tacgctgctt cttcatactg gtcatccatt
     1381 ttctcaagca ctggctgaac cgctcttacg atgaatggaa tcccaataaa cacaagtgcg
     1441 atcaaaagtc cgatccttgt ataagaaatc ttaatcccaa agtgtgcaaa gtatcgtccg
     1501 atcattccat cttctgcata catttttgat aatgtaatac cagcaactgc ggttggcagt
     1561 gcaaaaggaa gttcgatcat cccatccatt aatcgtttcc ccggaaattc atattttaca
     1621 agtacccatg cgatgatcag cccaaacaca acattgatca atgctgcaat aaacgaactt
     1681 ccgatacttg tgataaatgc atttcttact gtttctttcg atattaatgt aaagaattca
     1741 ttcggc
//
LOCUS       DAOMUE010000363         1743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_19183_length_1743_cov_1.841825, whole genome shotgun sequence.
ACCESSION   DAOMUE010000363 DAOMUE010000000
VERSION     DAOMUE010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19183_length_1743_cov_1.841825"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1154
                     /gene="pyrB"
                     /locus_tag="PLC71_11050"
     CDS             228..1154
                     /gene="pyrB"
                     /locus_tag="PLC71_11050"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRM03212.1"
                     /translation="MRHLIDPMDLSVEDIDRLLDRADDIAKNPEKYQEVCKHKKLATL
                     FFEPSTRTRLSFEAAMLELGGSVLGFSSADSSSASKGESVADTARVIECYADIIAMRH
                     PKEGAPYVASQYVNIPVINAGDGGHNHPTQTLTDLLTIRREKGRLSNFTIGFCGDLKF
                     GRTVHSLIKALSRYEGINYILISPEELQIPEYLKKDVFEKKGITFTEVDKMEDVMPQL
                     DILYMTRVQRERFFNEADYVRLKDSYILDMDKLKIAKKDLSILHPLPRVNEISVKVDN
                     DPRACYFKQVLNGKHVRMALIMELLGVSADEH"
     gene            1144..>1516
                     /locus_tag="PLC71_11055"
     CDS             1144..>1516
                     /locus_tag="PLC71_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HRM03213.1"
                     /translation="MNIDSIKNGIVLDHIRAGRGMQIYNSLGLDQLDCSVAIIKNVKS
                     SKMGKKDIIKISDAFEVNFDVLGYIDPEVTVCVIENGSVKSKHKVELPEKIVNIAKCK
                     NPRCITSVEQEIDHVFKLTDRE"
     assembly_gap    1517..1526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          631 a          249 c          387 g          466 t
ORIGIN      
        1 ccgatgatca atcaacagtt tgtgatccat gagatggaag gtttcaaata tgtgaacaga
       61 gaagacgatg ctggagaaga gggacttaga aagtcaaaat tatcgtatcg accagtcttt
      121 atggtagaca agggcgttct tcgtctaaaa tcagaagtaa agaattgaca ctaggtgcct
      181 aaaaatgtag aattgaaggt aggacaaacc attaggagga atatgtaatg agacatttga
      241 ttgatccgat ggatttaagc gtggaagata ttgatagatt attagaccgc gctgatgaca
      301 tcgcaaagaa tcctgagaag tatcaggaag tgtgcaaaca caagaaatta gcaacgctgt
      361 tctttgaacc aagtacaaga acaagattaa gctttgaagc agcaatgctt gaattagggg
      421 gaagtgttct tggattttct tctgctgatt catcatctgc atctaaagga gaaagtgttg
      481 cagatacagc aagagtcatt gaatgttatg cagatatcat tgcgatgcgt catccaaagg
      541 aaggagcacc atatgtagcg tcacagtatg taaatatccc tgtgatcaat gcaggagacg
      601 gtggtcataa tcatccaaca cagacattga cagacctttt aacaattcga agagaaaagg
      661 gacgtttaag taatttcacg attggatttt gtggagattt aaaatttgga cgaacagtgc
      721 attctctgat caaagcatta tcaagatatg aaggaatcaa ttatatttta atttctccag
      781 aagaactcca gattccagag tatctgaaga aagatgtttt tgagaagaaa ggaatcacat
      841 ttacagaagt tgataagatg gaagatgtga tgcctcagtt agatattctt tatatgacac
      901 gagtacagag agaacgtttc tttaatgaag ctgattatgt aagattgaag gatagctata
      961 tcttagatat ggataaatta aagatcgcca agaaagatct ttctatttta catccattac
     1021 ctcgtgtgaa tgagatttct gtaaaagttg ataacgatcc aagagcctgc tattttaaac
     1081 aggtattgaa tggaaaacat gtaagaatgg cattgatcat ggaattactg ggggtaagtg
     1141 cagatgaaca ttgatagcat taagaatgga atcgtattag atcatatccg tgcaggacgc
     1201 ggaatgcaga tttataatag tcttggatta gatcagttag attgcagtgt tgcgatcatt
     1261 aagaatgtaa aaagcagcaa aatgggcaag aaagatatca ttaagatttc agatgcattt
     1321 gaagtgaact ttgatgtttt aggttatata gatccagaag ttacggtatg tgtcattgaa
     1381 aatggcagtg taaagtccaa acacaaagta gaacttccag agaagatcgt gaatattgca
     1441 aaatgcaaga atccaagatg tattacatct gtagaacaag aaatcgatca tgtgtttaaa
     1501 ttgacagaca gagaacnnnn nnnnnngtat atcgctgtat ttactgcgaa tcaaagctta
     1561 aaaaataatc aaaagaaaca agaggtatcc gtgcatgaaa caagaaagca gaacaaacat
     1621 taatcgtgta aagaaggata tcagcagtcc agatagtttc acaccgcctg aggaactgaa
     1681 acgacagctg atcacaacca tgcaaagcat ttatgatgaa gaagatattg aggctgtaga
     1741 acg
//
LOCUS       DAOMUE010000364         1712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_19626_length_1712_cov_2.371153, whole genome shotgun sequence.
ACCESSION   DAOMUE010000364 DAOMUE010000000
VERSION     DAOMUE010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19626_length_1712_cov_2.371153"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1712
                     /locus_tag="PLC71_11060"
     CDS             <1..>1712
                     /locus_tag="PLC71_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HRM03214.1"
                     /translation="YMVEKVFYNPISVMNKLAFGMNILWYYILFFILLLIFNRVKWAL
                     LVGDVFLYAAAIGNYFVLAFRGTPITPADIYALGTAMDVADHYVLSYDKPAIVATVVL
                     LGLCVFACKLDTYKIFHWKKRLVALLITAIVTVGSSFFLTRVDFLSKKGVAVNFWQQK
                     KGYLKNGYILSFLMNIQYTIVSQPDGYSPEAVDKIADKYQVTQGTNKKLKQKPNVVVI
                     MNETFSDLNVVNKIKTNKEVMPFINSLSENTIKGHMLVSVFGGGTSNSEYEFLTGNSV
                     SSLPLNGNAYTQFVKHKVPSLASQLKQQGYDTLAFHPYKAHGWNRDTVYPLIGFDNFL
                     DETSMNPNGEKFRGWYSDAEDYNKIIDIFNKKKAGQPLFLFNVTIQNHGGYLIADKNF
                     KEEIKIKDEKATDTANRYLSLIHESDRAFEKIINYFKNQKEPTIVVMFGDHQPKLEDS
                     FYELLYGKSLNSLSLKELQKKYTVPFIIWANYDIDAKSDVENVSANYLSSLMLQQTNL
                     KMSRYNEFLLNMRNEVPALNANGYVDKDGKNHELSENNEYTKLITQYQYLQYNSLMDK
                     KHVST"
BASE COUNT          617 a          264 c          347 g          484 t
ORIGIN      
        1 tatatggtag aaaaagtatt ctataatccg atcagtgtga tgaataaatt ggcatttgga
       61 atgaacatcc tatggtacta tatattgttc ttcattttat tactgatctt taaccgtgtg
      121 aaatgggcgt tgctcgttgg agatgtattt ttatatgcag ctgcgatcgg aaactatttt
      181 gtattagcat tccgtggaac accgatcacg ccggcagata tttatgcact tggaacagca
      241 atggacgttg cagatcatta tgtattaagt tatgataaac cagcaatcgt tgcgacggtt
      301 gtattattgg gattatgtgt gtttgcatgt aaactcgata catataagat cttccattgg
      361 aagaaacgtc tagttgcatt actgatcaca gcaatcgtaa cagtaggaag ttctttcttc
      421 ttgacaagag tagacttttt gtcaaagaaa ggtgttgctg taaacttctg gcaacagaag
      481 aaaggatact taaaaaatgg atatatttta agctttttaa tgaatattca gtatacgatc
      541 gtttcccagc cagatggata tagcccagaa gcggtagata agatcgcaga taagtatcag
      601 gtaacacagg gaaccaataa gaaattaaaa cagaaaccaa atgttgtcgt gatcatgaat
      661 gaaacattct cagatctgaa tgtagtaaat aagatcaaga caaacaaaga ggttatgcca
      721 tttatcaata gtttaagtga aaatacgatc aaaggacata tgctggtatc tgtttttggt
      781 ggaggaacaa gtaattcaga gtatgagttc ttaacaggaa actctgtatc atctcttcca
      841 ttaaacggaa atgcatatac acagtttgta aaacataagg tgccaagtct ggcatctcag
      901 ttaaaacagc agggatacga tacactggca ttccatccat acaaagcaca cggatggaac
      961 cgagatacgg tatatccgct aattggattt gataatttct tagatgagac atctatgaat
     1021 ccaaatggag agaaattcag aggatggtat agtgatgcag aagattacaa taagatcata
     1081 gatatcttta ataagaaaaa agcgggacag ccattattct tattcaatgt aacgatccag
     1141 aaccatggtg gatatctgat cgcagacaag aactttaagg aagagatcaa gatcaaagat
     1201 gagaaggcaa cagatacagc aaaccgttat ttatcactga tccatgaatc agatcgagca
     1261 ttcgagaaga tcatcaacta ctttaagaat cagaaagagc caacgatcgt tgtaatgttt
     1321 ggagatcatc agccaaaact tgaggattct ttctatgagc tgttatatgg aaaatctcta
     1381 aacagcttaa gtttaaaaga actgcagaag aaatatacag ttccatttat catctgggca
     1441 aattatgata ttgatgcaaa gagcgatgtg gaaaatgtca gtgcaaacta tttatcaagc
     1501 ctgatgttac agcagacaaa cttaaagatg tcaagataca atgagttctt attaaatatg
     1561 agaaatgaag tcccagcatt aaatgccaat ggatatgttg ataaagatgg aaagaaccat
     1621 gagttgtctg agaataatga atatacaaaa ctgatcacac agtatcagta tctacagtat
     1681 aacagtctga tggataagaa acatgtaagt ac
//
LOCUS       DAOMUE010000365         1710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_19667_length_1710_cov_2.039275, whole genome shotgun sequence.
ACCESSION   DAOMUE010000365 DAOMUE010000000
VERSION     DAOMUE010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19667_length_1710_cov_2.039275"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLC71_11065"
     CDS             <1..343
                     /locus_tag="PLC71_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein"
                     /protein_id="HRM03215.1"
                     /translation="DNLVDDAEDAVDDVADGVGDAVDDIANGFDNYDDAHDYLLNRLQ
                     NDDKDGKYEVRNETKDEQEYRDGASGYHFEIYDTSDDSGKKYGDFYVDKDTGKIYKRN
                     TKTEKLEEYKK"
     gene            complement(362..793)
                     /locus_tag="PLC71_11070"
     CDS             complement(362..793)
                     /locus_tag="PLC71_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03216.1"
                     /translation="MSSKTKIVVLKARELIYTGIFILLGILLILLLIFMFVPSHKSKK
                     TKETSGYTQGVYSSPLKLGENELELSVTVKDGKARSISLKHLNKSIKTMYPLIEPSLN
                     EINKQLPKVQNIDDIKFDGQSQYTYTLLKQAIKNALKKAQD"
     gene            911..1081
                     /locus_tag="PLC71_11075"
     CDS             911..1081
                     /locus_tag="PLC71_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03217.1"
                     /translation="MALQFILGSARSGKTDFIYDQMIKDSMEHEDEEFFFIVPDQSTL
                     NAQKQLVTRHPH"
     gene            1044..>1710
                     /locus_tag="PLC71_11080"
     CDS             1044..>1710
                     /locus_tag="PLC71_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HRM03218.1"
                     /translation="MHKNNWSQDIHIDVVGFFRLTYRVFEELSYIPKDLLEDEGKSMV
                     IRKIMEQHKEELKVFASSMKKQGFIDELKSFFAEVYQYDVSMEDLKKITDGMKEEGLR
                     SKMEDILLVMENFEDYIKERYLISEQLLDVLAQKVEQSEKLKNATFYLDGFTGFTPIQ
                     RKVLEKLLKIGKNVYVGLTLDGRYVRSQYREYELFAMSKKEKYELIKLAQDAGTPVMP
                     DIIC"
BASE COUNT          604 a          228 c          373 g          505 t
ORIGIN      
        1 agataatctg gtagacgatg ctgaggatgc cgttgatgat gtggcagatg gagttggaga
       61 tgcggtagat gatatcgcaa atggttttga taactatgac gatgcacatg attatttatt
      121 aaaccgtctg caaaatgacg ataaagatgg taagtatgaa gttcgaaatg agacaaaaga
      181 cgaacaggaa tacagggatg gagccagtgg atatcatttt gaaatctatg atacatccga
      241 tgattctggg aagaaatacg gagatttcta tgtagataaa gatacaggaa agatctacaa
      301 acgaaataca aaaacagaga aattagaaga atataagaaa taaaaagaag gcacaggggt
      361 gttaatcctg tgccttcttt aatgcatttt tgatcgcttg ttttaataat gtatacgtat
      421 actggctttg cccgtcaaat ttgatatcat caatattttg aactttggga agttgtttat
      481 tgatctcatt tagagaaggt tcgatcagag gatacattgt tttgatcgat ttatttaaat
      541 gttttaaaga aatcgatctt gctttgccgt cctttaccgt gaccgaaagt tctaattcgt
      601 tttctccaag ttttaatgga gaagaataga caccttgtgt gtaaccggaa gtttcttttg
      661 ttttctttga tttatgggaa ggaacaaaca taaagatcaa aagtaagatc aaaagtattc
      721 caagtaagat aaagattccg gtgtaaataa gttcccgtgc tttcaatacg acaattttcg
      781 tttttgatga catagttatc ctcctaaaag attctatcta tagtttatga atggaatgag
      841 atatttagaa catctttacg gaaaataaag gataagatat aataaaatga tataaaatgg
      901 aggaaatgat atggcattac agtttatact agggtctgcg agatccggaa aaacagattt
      961 tatttatgat cagatgatca aagactccat ggaacacgaa gatgaggagt tcttttttat
     1021 tgtgccggac cagtcaacgc taaatgcaca aaaacaactg gtcacaagac atccacattg
     1081 atgtggtggg atttttcaga ttaacttatc gtgtattcga agaattatca tatattccga
     1141 aagacctttt ggaagatgaa ggaaaatcta tggtgatccg aaagatcatg gagcagcata
     1201 aagaagaatt aaaagtattt gcgtccagca tgaaaaaaca gggatttatt gatgaattaa
     1261 aatctttttt tgcagaggtc tatcagtatg acgtgtccat ggaagattta aagaagatta
     1321 cagatgggat gaaagaagaa ggattacgct caaagatgga agatattctt cttgtgatgg
     1381 agaattttga agattatatc aaggaacggt acttgatctc tgaacaattg ttagatgttc
     1441 tggcacagaa agtggaacaa tcagagaaat taaagaatgc aacattctac ctggatggat
     1501 ttacaggatt tacgccgatc cagagaaaag tactggaaaa gctattaaag attgggaaaa
     1561 atgtatatgt tggactgact ttagatgggc gttacgtgag aagtcagtac cgtgaatatg
     1621 aattatttgc catgtcaaag aaagaaaaat atgaactgat caaacttgcg caggatgcag
     1681 gaaccccagt gatgccagat atcatctgtt
//
LOCUS       DAOMUE010000366         1705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_19751_length_1705_cov_1.681212, whole genome shotgun sequence.
ACCESSION   DAOMUE010000366 DAOMUE010000000
VERSION     DAOMUE010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19751_length_1705_cov_1.681212"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            678..>1485
                     /locus_tag="PLC71_11085"
     CDS             678..>1485
                     /locus_tag="PLC71_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRM03219.1"
                     /translation="MKNRFLALLICAILVFSIIPFGKNIKASQTNNNAKQVNTPEQMA
                     AVMREKANNNTARMKGKITQSQVNETIKQKLIVKTRDEIENTYGANSVYYYTVGNYQI
                     LFYDSAEEAKNACEKLKKDLPGTTIFQDIPITLKENGSDEVAAYDGIKKMGMDQLKEE
                     KDSWKNKSAKVAVIDSGINVDHQWFKNRLDRENSINLALDEGNEDDKTKPAYSDTKQG
                     HGSHVAGIITQATPEEVQVMAIRVFSALGTASYATITNAVDYAVEHKADII"
     assembly_gap    1486..1495
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1517..>1705
                     /locus_tag="PLC71_11090"
     CDS             1517..>1705
                     /locus_tag="PLC71_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03220.1"
                     /translation="MDEAFARAFKANTTVCAASGNEYTDTSKSYPASSPWTIAVGSFE
                     PDAKGNLIRSDFANNGELL"
BASE COUNT          701 a          220 c          327 g          447 t
ORIGIN      
        1 tatataataa atgatgatga aaatgtagct actaatagaa tagaatctgt ggaatggata
       61 taaatggaat aataaggaat aataaggctg aaagctgctt tacatcaaca cctaggaatg
      121 aagcaagaaa gcaattgaga atcattatca attactgacc atatgttgca gtgatgcaac
      181 aagatggacg aaatgaatat gatgtatcgg aaagatattg tcatttttaa agagaaatag
      241 taaaacatct tttggttgaa cataagaata caatatggta aaaataaaac agaacatgaa
      301 ccttcggtga gtgtaaaata atagctaaca aaaagaaaaa gaatctaaga gtgcgatctt
      361 gggttctttt tcttttttat ctacaatgga aaaataacag ataaaaattc agattttttg
      421 tctgtatcaa aaaaataaaa tgagaaaaac tcttattaac aataattttg ttgcatcaat
      481 gcaacttcag aaaaattaaa aaaagaacgt aaatatcaag aaagtgaaaa ttttgtgaat
      541 ttttcacttt ctttttttgt tctctttgaa aaatttgttg tgattgacat gaaaataata
      601 gttatggtaa atattagtca aaactaactt taaaaatatt ataaattgat tacaacaagg
      661 gaggaaaaga gaaacttatg aaaaatcgtt ttttagcatt attaatctgc gcgattttgg
      721 tattttcaat tattccattt ggaaaaaaca taaaagcatc acagacaaat aataatgcaa
      781 aacaagtaaa tacgccagaa cagatggctg cagttatgag agaaaaagca aataataata
      841 cagccagaat gaaaggaaaa ataactcaat ctcaggtcaa tgaaacgatt aaacaaaaat
      901 tgatcgtaaa aacaagagat gagattgaaa atacttatgg tgctaacagt gtgtactact
      961 acacagtggg aaattaccag attttgtttt atgactctgc agaagaggca aagaatgcat
     1021 gtgagaagtt aaaaaaggat cttccaggaa caacaatatt tcaagatata ccaatcacac
     1081 taaaagaaaa tggatcagat gaagtggcgg catacgatgg aattaaaaaa atgggaatgg
     1141 atcaacttaa ggaagaaaaa gacagttgga aaaataaaag tgccaaagtt gccgtgatcg
     1201 acagtggaat taatgtggat catcaatggt ttaagaacag gctagatcga gaaaacagta
     1261 taaatcttgc gttggatgaa ggaaatgaag atgataaaac gaagccagca tatagtgata
     1321 caaaacaagg acatggaagt catgtcgcag ggatcattac gcaggcaaca ccagaagaag
     1381 tacaagtcat ggcaattcga gtatttagtg ctcttggtac agcatcctat gcaacgatca
     1441 caaatgcggt ggattatgca gtagaacata aagcagatat tattannnnn nnnnngaaaa
     1501 tgatcagata actctaatgg atgaagcgtt tgcaagggca tttaaagcaa atacaacagt
     1561 atgtgctgcg tcaggaaatg aatacacaga tacttctaaa agttatccag cgagcagtcc
     1621 atggacgatc gcagttggat catttgagcc agatgcaaaa ggtaacctga ttcgttcaga
     1681 ttttgccaat aatggagaac tactt
//
LOCUS       DAOMUE010000367         1693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_19922_length_1693_cov_2.179487, whole genome shotgun sequence.
ACCESSION   DAOMUE010000367 DAOMUE010000000
VERSION     DAOMUE010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19922_length_1693_cov_2.179487"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="PLC71_11095"
     CDS             <1..799
                     /locus_tag="PLC71_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HRM03221.1"
                     /translation="QYMSKEISKQEKEDYLSLMYEMKRKENQIVKSDLSRELEVPLSR
                     VTRVTDGLLEEGYLLKDEGRRMFLTPMGLSKGQQCLERKRCLTEFLRLVSGVDGSIAK
                     ENACAIEHVLDERILTGIRMFMESRHTYSYMTRGNDLNLMFPEGKRIMPIAFFEKGTS
                     HPRILSKEYQQFEKRAEVVISKESYLYLKPIDSDLLKKEIRYCYGNQWMYAKKENGKF
                     GILTEALDCTIRRNDKLSEGIVEIVVCDHHEEEIIEESVRILTVSLI"
     gene            810..1184
                     /locus_tag="PLC71_11100"
     CDS             810..1184
                     /locus_tag="PLC71_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM03222.1"
                     /translation="MCDKTMENYKPNMFQLQCLKALEIQIEEGKGYNEAEIGRKMQVN
                     RSTISRCFKRYREEGFLEDKGFTRKGAEFLEYYKMIESDLYHYFASIGLTNNSKDRQL
                     QECLILQISIRSRNYARKRKCI"
     gene            1121..1654
                     /locus_tag="PLC71_11105"
     CDS             1121..1654
                     /locus_tag="PLC71_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03223.1"
                     /translation="MFDTADIDTIQKLCQKEKMHLRYESIGKNESKEITKLTYEQLCN
                     SLKKGIYQVAFSIYRQGKDWQSLSMADQGFEKPAYLHLKGDKNYLELAVREMKAMTKG
                     GAMLSGHIQTVKCRSSKDVLTDLPIQDKKIQIPFEDFEFEQLSETEITGQIQLFMTSS
                     VGEKRMPESMATLIVKL"
BASE COUNT          661 a          238 c          374 g          420 t
ORIGIN      
        1 acaatatatg tcaaaagaga tatcaaaaca ggaaaaagaa gattatctaa gtctaatgta
       61 tgaaatgaaa cgaaaagaga accagattgt aaaatccgac ttatccaggg aacttgaagt
      121 gccgttatca agagttacca gagtcacaga tggattgtta gaagaagggt atctgttaaa
      181 ggacgaagga agaagaatgt tcttaacgcc gatgggatta tccaaagggc agcaatgtct
      241 ggaaagaaaa cgatgcctta cagagtttct gcgtttggtt tctggtgttg atggaagtat
      301 tgcaaaagaa aatgcatgtg cgatcgaaca tgttctggat gaacgcattt taaccgggat
      361 ccgtatgttt atggaaagcc gtcatacata ttcatatatg actagaggaa atgatttaaa
      421 tctgatgttt ccagaaggaa agaggatcat gccgatcgca ttttttgaaa aaggaacatc
      481 acatccaaga attctttcga aagaatacca gcagtttgag aaacgagcgg aagtagtgat
      541 cagcaaagaa agttacttgt atttaaaacc gatagattca gatctgctga aaaaagaaat
      601 tcgctactgt tatggaaatc agtggatgta tgcaaaaaaa gaaaatggaa aatttggaat
      661 tttgacagag gcattggact gtacgattcg aagaaacgac aaattatctg aaggaattgt
      721 agaaatagta gtgtgtgatc atcatgaaga agagattata gaagaatcgg ttcgtatttt
      781 aaccgtatca ttaatttagg agtttgacaa tgtgtgataa aacaatggaa aattataaac
      841 cgaatatgtt tcagttacaa tgcttaaaag cattagagat acagattgag gaaggaaaag
      901 gatacaacga agcagagatc ggaagaaaga tgcaggtaaa cagatcaaca atctcccgat
      961 gcttcaaaag ataccgagaa gaaggattcc tggaagataa aggatttacc agaaaaggag
     1021 cggaattctt agaatattac aaaatgatcg aatcagatct ttaccattac tttgcatcta
     1081 tcggattaac gaacaacagc aaagacaggc agttacagga gtgtttgata ctgcagatat
     1141 cgatacgatc cagaaattat gccagaaaga gaaaatgcat ctaaggtatg aaagtatcgg
     1201 aaaaaatgaa agtaaagaaa ttaccaagtt aacctacgaa caattatgca atagtttaaa
     1261 aaaaggcata tatcaagtcg cattttccat ttatcgacaa ggaaaagact ggcaaagtct
     1321 ctctatggca gaccaaggat ttgaaaaacc agcatatctg catttaaagg gggataaaaa
     1381 ctatttagaa ctagccgtca gagaaatgaa agcaatgaca aaaggtggag cgatgctgtc
     1441 tggacatatt cagacagtca aatgtagaag cagcaaagac gttttaacag atcttccgat
     1501 ccaagataag aaaatacaga ttccatttga agattttgag tttgagcagt tgtcagaaac
     1561 agaaatcacc gggcagatcc aactgtttat gacaagcagc gttggggaga agagaatgcc
     1621 agagagtatg gccacgttga tcgtaaaatt atagaaatat gaattatggg aattttaatt
     1681 aaaaattaaa att
//
LOCUS       DAOMUE010000368         1667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_20326_length_1667_cov_1.841811, whole genome shotgun sequence.
ACCESSION   DAOMUE010000368 DAOMUE010000000
VERSION     DAOMUE010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20326_length_1667_cov_1.841811"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PLC71_11110"
     CDS             complement(<1..912)
                     /locus_tag="PLC71_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HRM03224.1"
                     /translation="MRKTKYHIKKKIMPLLLAVAMAGTATPIYVAHPAIVKAAVSNAF
                     GTKENTIHIAQFGGYDIKTTVTYKDGKVTDLQIKGENFEGKYAEENENIYLPKAINKI
                     KDQIQGLDITDQNSFDKVDTVSGATTSASAIKNAVMESLGLTSKEEVLAPAPETVEEG
                     TYEIQMKNMTSTVEHSLSAANSENKVTATLKVDKNGKMTLSYPVVTKEAMDVLAFNGY
                     YNGSDLTKEGSEETKDGESVTTVNMPLTGDKPELTYKANFKMYVPAMSNLSGEHGGIT
                     FDHGKFDTDAVITLYWDTLKEKKDKEVL"
     gene            complement(1147..>1667)
                     /locus_tag="PLC71_11115"
     CDS             complement(1147..>1667)
                     /locus_tag="PLC71_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRM03225.1"
                     /translation="SNLGTYRIKKVLNATLNGTKQYRFTVYSNELAVSKNDDKKPSNT
                     TAEQKPSSATTEQKPSDTTTTQKLSMTTVKDILKKVSKINLQNLKKRKVRITWKKVKN
                     ADGYQVYRATKKNGKYKLVKVVKGNKKVSYTNTKLKKNKKYYYKVRAYRTVKGKKVYG
                     AFSSKKSILIKK"
BASE COUNT          403 a          309 c          231 g          724 t
ORIGIN      
        1 aagcacttct ttgtctttct tttcttttaa tgtatcccaa tataatgtga tcacagcatc
       61 tgtatcaaat tttccatgat caaatgtaat tccgccatgt tcaccactta aattgctcat
      121 tgctggtaca tacattttaa agtttgcttt ataagttaat tctggtttat cacctgttaa
      181 tggcatattt acagttgtta cactctctcc atcttttgtt tcttcggatc cttcttttgt
      241 cagatctgat ccattataat acccattaaa agctaataca tccattgctt cttttgtaac
      301 cactggatat gataatgtca tctttccatt tttatcaacc tttaatgttg ctgttacttt
      361 attctcagaa tttgctgcag acaagctatg ttctactgta cttgtcatat ttttcatctg
      421 aatctcataa gtaccttctt ccactgtttc tggtgctggt gcaagaactt cctcttttga
      481 tgttaatcct agagattcca taactgcatt tttaatcgcg gaagcacttg tcgttgctcc
      541 agacactgta tcgactttgt caaaggaatt ctgatctgtg atatctagac cttgtatctg
      601 gtctttgatt ttattgatcg cttttggaag gtagatattt tcattttctt cagcatattt
      661 tccttcgaaa ttttctcctt taatctgaag atctgtcact tttccatctt tgtatgtaac
      721 agttgtttta atatcgtatc caccgaactg tgcaatatga atcgtatttt cttttgttcc
      781 aaatgcatta gacactgctg cctttacaat tgctggatgt gctacatata tgggtgtcgc
      841 tgttccagcc attgcaacag ctaacaataa tggcatgatt tttttcttta tatgatattt
      901 agtttttctc ataatctctc cttctcttaa tttataggca ttatcggtta gttttaatta
      961 actcacttct tttttatcat gcatcaatat tctccgtcaa cgaaaacaag tttttcaaaa
     1021 tcgttcatat ctgatatttt aaatttaaaa tttctttaaa aaaacacgtg tttctgccat
     1081 ttatcaataa aacacaaggt gttgcaggcg tgcaacccct tgtgttttat tgataattct
     1141 tctcatttat tttttgatca atatgctttt ctttgaggag aatgcaccat atactttctt
     1201 acctttaact gtacgataag cacgaacttt gtagtaatat tttttatttt tctttaactt
     1261 tgtatttgtg taacttactt ttttattacc ttttactacc ttaacaagct tatattttcc
     1321 gtttttcttt gttgctcgat aaacttgata tccatctgca ttctttactt tcttccatgt
     1381 aatacgcacc ttacgttttt tcagattttg cagattgatc tttgacactt tcttaagaat
     1441 atctttcact gttgtcattg ataatttttg tgtcgttgtt gtatccgatg gcttttgttc
     1501 agttgttgca cttgatggtt tctgttcggc tgttgtattt gatggttttt tatcatcgtt
     1561 tttagatact gctaattcgt ttgaatatac agtaaaacgg tattgttttg ttccatttaa
     1621 tgtcgcattt aagactttct ttattctgta tgttcccaga tttgata
//
LOCUS       DAOMUE010000369         1661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_20416_length_1661_cov_1.184932, whole genome shotgun sequence.
ACCESSION   DAOMUE010000369 DAOMUE010000000
VERSION     DAOMUE010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20416_length_1661_cov_1.184932"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLC71_11120"
     CDS             complement(<1..204)
                     /locus_tag="PLC71_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03226.1"
                     /translation="MMIIGNLFMSIPSPSFSYGWFVIFSVIISAIMWSMFHQGMSLLK
                     ELEKHHLKEKHQQMLLEEEIKHLH"
     gene            complement(822..>1661)
                     /locus_tag="PLC71_11125"
     CDS             complement(822..>1661)
                     /locus_tag="PLC71_11125"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HRM03227.1"
                     /translation="HKLQLFLIMIYIFILPNIAVIYHYLYINPTFKEYLAKTSLHPYF
                     YFCFNLLMVACLLSFISIIVITCKNTKKSIELDMLDSTQQTILKQEDEMKLLQEQILA
                     INLCESEKYEEMTTLISALSSHVKRNYPDTYCKNILLNTLLQEKKFLADQLDVTCNFK
                     IILPEHFEEDFSDFTITSIFSNLLDNALESCKQYREHHPQNDSLFIHLSTDYKTKMFM
                     IQMKNSKDPDIPFTRQSTKDDSSLHGHGLSIIESIAKDYGGTCQWSVEDDSFLSLIML
                     KHT"
BASE COUNT          653 a          236 c          285 g          487 t
ORIGIN      
        1 atgcagatgc ttgatttctt cttctaagag catttgttga tgtttttctt ttaaatgatg
       61 tttctctaat tcttttaaca acgacatacc ctgatgaaac atactccaca tgatcgcaga
      121 gatgatcact gagaatataa caaaccatcc gtaagaaaag gatggagaag gtatggacat
      181 aaacagattt ccaatgatca tcataaaaag aacaggaatc cctagataaa gaaacaattt
      241 taaattgatc aaatgacgaa aacgctgaat aaagtgaaaa aggcgtggca agaacagata
      301 atttaaaatg atctgaaata tagaacataa agcgattgaa atcgtgttac cacgcaaagc
      361 aacatataaa acagctagat gaagctgtgg aaatataaag tttaagatca ctaatattcc
      421 agcagaaact gcctctacaa aaatcatatt tacaataaaa atgaaaaata aaaagatccg
      481 aaaaggaaga tcttccgtga aaaagaaaaa aatatgaaag ctccataatg gataaatcat
      541 gaaagataaa aagacagcgg atttagcaac tggaacaaaa atcatccaga gaagagaaat
      601 cgcataaaat gaccataaga ctctgaaaaa taaaggagag aaatctttat gagaaaatag
      661 ttttaaaaat atatgaaacg gataaatgat gttaaaaatg cttacaatat aaaaaaataa
      721 cccagcgatc ataaattgta ctcctccctt atctcgtcag atattattat ataacgaatc
      781 aaaatgcaga aataaaatga atattacata taaaattgtt tttatgtatg ttttaacatg
      841 attaatgaca aaaaagaatc atcttcaaca ctccactgac aagttccgcc atagtccttg
      901 gcaatacttt ctatgataga aagtccatgc ccatggagtg aggaatcgtc ttttgtactt
      961 tgtctggtaa aaggaatgtc aggatcttta gagtttttca tttggatcat aaacatcttg
     1021 gtcttgtaat ctgtggacaa atgaataaaa agagaatcat tttgtggatg atgctcgcga
     1081 tactgtttgc agctttccaa ggcattatca agtaaatttg aaaagatact tgtgatcgta
     1141 aaatctgaaa aatcttcttc aaaatgttct ggaagaataa tcttaaagtt gcaggtaaca
     1201 tcaagttgat ctgcaaggaa ttttttctcc tgaagaagcg tatttaaaag aatatttttg
     1261 caataagtat ctggataatt tctttttaca tgagaagaaa gtgcagagat taaagtcgtc
     1321 atttcttcat acttttcaga ttcacaaaga ttgatcgcaa gaatctgctc ctgtaataac
     1381 ttcatttcat cttcctgctt taagatggtt tgttgtgtgg aatcaagcat atcaagctcg
     1441 atcgatttct ttgtattctt acaagtgatg acgatgatgc tgataaagga aagaaggcag
     1501 gcaaccatca gtaaattaaa gcaaaaataa aaataaggat ggagagatgt ttttgctaag
     1561 tattctttga acgttgggtt aatatataaa taatggtata taactgcaat atttggcagg
     1621 ataaagatat agatcatgat caaaaataat tgcaatttat g
//
LOCUS       DAOMUE010000370         1652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_20545_length_1652_cov_2.324358, whole genome shotgun sequence.
ACCESSION   DAOMUE010000370 DAOMUE010000000
VERSION     DAOMUE010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20545_length_1652_cov_2.324358"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLC71_11130"
     CDS             complement(<1..224)
                     /locus_tag="PLC71_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HRM03228.1"
                     /translation="MHTKRIIPCLDVKAGRVVKGVNFVNLIDAGDPVEIAAAYDKAGA
                     DELVFLDITASSDARNIMIDMVRKVAEKVF"
     gene            complement(237..842)
                     /gene="hisH"
                     /locus_tag="PLC71_11135"
     CDS             complement(237..842)
                     /gene="hisH"
                     /locus_tag="PLC71_11135"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRM03229.1"
                     /translation="MIAIIDYDAGNIKSVEKALNYLGEEAIITRDHDEIVNSDKVILP
                     GVGAFADAMEKIRHYGLEETIHEVVEKNIPFLGICLGLQLMFESSDEGPGVKGLGLLP
                     GKILRIPKSGDLKIPHMGWNNIKVKEDSRLFKGLPENPYVYFVHSYYLQADDEDIVAA
                     TTEYGVKIHAAVEKDNIFACQFHPEKSSTVGLQILKNFVSL"
     gene            complement(852..>1652)
                     /locus_tag="PLC71_11140"
     CDS             complement(852..>1652)
                     /locus_tag="PLC71_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03230.1"
                     /translation="IKRMKRNMLKDYKEQIQDADLVLVGIGRELRADRVIDFKKAITN
                     EHYQNLIDKEDEDSKWMRTVYEREYLLSMKEIDLFKELEEVLEGKEYFVVTSNDDGLL
                     YHTHLKKDHVTAPCGNGDFFQCSAPCDEQLYPANLGLRDLIDYYEKTGKIEHLECPKC
                     GKQLIFNVRTEETKSIYIEGAYLNSWASYTKWLQNTLNKKLFILELGEGFEVPSLFRW
                     PFEKMAFYNEKATFVRVHHSLYQIGKEIKEKGIGIKMDSRDFLNIAHE"
BASE COUNT          423 a          374 c          252 g          603 t
ORIGIN      
        1 ataaatacct tttctgcgac ttttcttacc atatcgatca tgatatttct tgcatcggat
       61 gatgctgtga tatctaagaa tacaagttca tctgcgcctg ctttgtcata tgctgctgcg
      121 atctctactg ggtctcctgc atcgatcaga ttgacaaaat tgacaccttt tacaacgcgt
      181 cctgctttca catctaagca tgggataatt ctctttgtat gcatagccaa ttcctcctat
      241 agtgaaacga agttctttaa aatctgtaat cctacggtgc tgctcttttc cggatggaac
      301 tggcaggcaa agatattgtc tttctctact gctgcgtgaa tcttcacacc atattcggtt
      361 gtcgctgcta cgatatcttc atcgtctgcc tgaagatagt aagaatgtac aaaatataca
      421 taaggattct ctggcagtcc tttgaataat cttgaatctt ctttgacttt gatattattc
      481 catcccatat gtggaatctt taaatctcca ctctttggaa ttcttaagat ctttcctggt
      541 aataatccaa gtcctttgac tcccggtcct tcatcgctgg attcaaacat cagctgtaac
      601 ccaagacaga ttcctaaaaa tggaatattc ttctctacaa cttcatggat cgtttcttct
      661 aatccataat gacggatctt ctccatcgca tctgcaaatg ctccaactcc cggaagaatc
      721 actttatcgc tgtttacgat ctcatcgtga tctcttgtga tgattgcttc ttctccaaga
      781 taattgagtg ctttttcaac acttttgata tttcctgcat cataatcaat aatcgctatc
      841 ataggtgcct cctattcatg tgctatgttt aaaaagtcac ggctgtccat cttgatcccg
      901 attccttttt ctttgatctc ttttccgatc tggtatagtg aatgatgaac tcttacgaat
      961 gttgctttct cattgtagaa tgccatcttc tcaaaaggcc agcggaacaa acttggaact
     1021 tcaaaacctt ctcctaattc taatataaaa agctttttat ttaaagtatt ttgcagccat
     1081 tttgtgtaac ttgcccatga atttaagtaa gctccttcga tatagataga ttttgtctct
     1141 tctgtgcgga cattaaaaat caattgtttt ccacattttg gacattccag atgttcgatc
     1201 tttcctgttt tttcataata gtcgatcaaa tctctaagtc ctaagttcgc aggatagagc
     1261 tgctcatcac atggcgcact gcactggaag aaatctccgt ttccacatgg tgctgtcacg
     1321 tgatctttct ttaaatgggt atgatacaat aatccatcat cgttacttgt cactacgaaa
     1381 tattcttttc cttctaaaac ttcttctaat tctttaaaca gatctatctc tttcatggat
     1441 aataaatact ctctctcgta aacggttctc atccatttgg aatcttcatc ttctttatcg
     1501 atgagattct ggtaatgttc atttgtgatc gctttcttga aatcgatcac acggtctgca
     1561 cgcaattctc gtccaatccc cactaataca aggtctgcat cctgaatctg ttctttgtaa
     1621 tcttttaaca tatttctttt catccttttt at
//
LOCUS       DAOMUE010000371         1648 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_20611_length_1648_cov_2.293158, whole genome shotgun sequence.
ACCESSION   DAOMUE010000371 DAOMUE010000000
VERSION     DAOMUE010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20611_length_1648_cov_2.293158"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="PLC71_11145"
     CDS             <1..1000
                     /locus_tag="PLC71_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase"
                     /protein_id="HRM03231.1"
                     /translation="YGNITMEHECCTAEATAIAQAAKNCGGKVIVQVEKVVTDVDPKL
                     VKVPGIYVDAVVVTENKENHTQCVGCEYDGSMTGEFRVPLGSLEAPSLSPKKIIGRRA
                     AMELRPDTVVNLGIGIPEYISMVANEEGIGDHFTLTVEAGPVGGVPQGGPQFGGAVNA
                     QAILDQPAQFDFYDGGGVDDAFLGLAQADKDGNINVSKFGPRIAGCGGFVNITQNAKR
                     VYYCGTFTAGGLKCSTKDGKLIIDQEGKSDKFIDTVEQITFSGKYANKVGQPVMYITE
                     RAVFELREDGVYLTEVAPGIDIQTQILDHMGFVPKMDGEPKLMDERIFKDEIMGLE"
     gene            1032..>1648
                     /locus_tag="PLC71_11150"
     CDS             1032..>1648
                     /locus_tag="PLC71_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HRM03232.1"
                     /translation="MNIHILVLVIAIILFGLLAFKQMSALILAPLVTSFVIICSGMPI
                     LDSLKNLFMPAAAEYVTSYFLVFFVGALFGAVYQYTGAAESIARAIASLCHGKFVAPI
                     IMIITGLLTFGGVSGFVVFFVIYPIALNLFKEANLTRRLIPAAISAGCWTWSMSGPGS
                     PSVQNVIAMDSLGTPSTAAFVPSLLTAIAMFLMIFFWLEFRARSFT"
BASE COUNT          435 a          320 c          373 g          520 t
ORIGIN      
        1 atatggaaac attacaatgg aacatgaatg ttgtactgct gaagctactg cgatcgcaca
       61 ggctgcaaaa aactgtggtg gtaaagttat cgtacaggta gagaaagttg tcacagacgt
      121 tgatccaaaa cttgtaaagg tccctggaat ctacgtagat gcggttgttg tcacagagaa
      181 caaagaaaac catacacagt gtgtcggatg tgaatatgat ggttctatga ctggagaatt
      241 tcgtgttcct cttggaagcc ttgaagctcc ttctctttcc cctaagaaga tcattggacg
      301 tcgagctgcg atggaattac gtcctgacac cgttgtaaac cttggaattg gtattcctga
      361 atatatctct atggttgcaa atgaagaagg tatcggagat cactttactt taacagtaga
      421 agctggtcct gtcggtggtg tacctcaagg tggacctcaa tttggcgggg ctgtaaatgc
      481 tcaggcgatc ttagaccagc ctgcacagtt tgacttctat gatggtggtg gagtcgatga
      541 tgcattctta ggtcttgctc aggcagataa agatggaaat atcaatgtca gcaagtttgg
      601 accacgtatt gcaggatgcg gtggtttcgt taatattact cagaacgcta aacgtgtata
      661 ctactgcgga acatttactg ccggtggttt aaaatgttct acaaaagacg gaaaactgat
      721 cattgaccag gaaggaaaat ctgataaatt cattgatacc gtagaacaga tcactttctc
      781 tggtaaatat gccaataaag ttggccagcc agttatgtat atcacagaac gtgctgtctt
      841 tgaacttcga gaagatggcg tttatctgac tgaagttgca cctggaattg acatccagac
      901 tcagattctt gaccatatgg gattcgtacc aaaaatggat ggcgaaccga aactcatgga
      961 tgaacggatt tttaaagatg agatcatggg tcttgaataa ctagtttatt gtcccccaaa
     1021 aggagattta tatgaatata catattttag tattggtcat tgctatcatc ttatttggtt
     1081 tattagcttt taaacagatg agtgctttga tcctcgctcc tcttgtaact tcctttgtga
     1141 tcatctgctc cggcatgccg attcttgaca gtttaaaaaa cttattcatg cctgccgcag
     1201 cagaatatgt cacaagttac tttttggtat tctttgtagg agctttgttc ggagctgttt
     1261 accagtatac tggtgcagca gaatcgatcg cacgtgcgat cgccagttta tgtcacggaa
     1321 aatttgtagc accgatcatt atgatcatca ctggtttatt aacctttggt ggtgtcagcg
     1381 gattcgttgt attctttgtt atttacccta tcgcacttaa tctgttcaaa gaagcaaatc
     1441 ttacccgccg cctgatccct gctgccatct ctgcaggatg ctggacttgg tctatgagtg
     1501 gacctggttc tccttctgta cagaatgtta tcgctatgga tagtcttgga acaccatcta
     1561 cggctgcgtt tgttccatct ttgttaactg cgatcgcaat gtttttgatg attttcttct
     1621 ggttagaatt tcgtgcccgt tcttttac
//
LOCUS       DAOMUE010000372         1645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_20664_length_1645_cov_2.149686, whole genome shotgun sequence.
ACCESSION   DAOMUE010000372 DAOMUE010000000
VERSION     DAOMUE010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20664_length_1645_cov_2.149686"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>362
                     /locus_tag="PLC71_11155"
     CDS             <1..>362
                     /locus_tag="PLC71_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRM03233.1"
                     /translation="NKKNELKYLKKQLDEKDNISNRISEFRKNIMNNPILEEFDRVVF
                     ESIVEKAIVGGFDENGNKDPYKITFIYKTGIQDEIKEAKKKFVAKAYEQKEKDTEMYS
                     FSSNDQNNMCTNDNGNIN"
     assembly_gap    363..372
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <373..1352
                     /locus_tag="PLC71_11160"
     CDS             <373..1352
                     /locus_tag="PLC71_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRM03234.1"
                     /translation="LHVIPVTFLIPGVFLMCCFISTSIGTSMGTIAAMAPIAINVAQG
                     AHLNVAIVGAAVIGGSYFGDNLSMISDTTISAAEGCGSEMKDKFKMNFFIALPAAIVA
                     MILYSVVGGTGSGAIKAGSYNIIEVLPYLIVLGAALMGINVAVVLFVGILSAGIIGLC
                     VGSCGFFDWIQAIGNGMSDMFSISMVAILVSGIIGIVREYGGVEWLVGAISGKIKNRR
                     GAEYGIGLMSGILSAAMVNNTIAIIVTCPIAKEIGEKYKIAPKRLASLVDIFACAFIS
                     LMPHDGGMLMITALADVSPLNVLKYSFYMFGLIIATCVTIQFGLMDEKN"
BASE COUNT          545 a          218 c          371 g          501 t
ORIGIN      
        1 aaataaaaag aatgagttaa aatatcttaa gaaacaatta gatgaaaaag ataatatttc
       61 taacagaatt agtgaattta gaaagaatat aatgaacaat ccgattctgg aagaatttga
      121 tagagttgtt tttgaaagta tagttgaaaa agccattgta ggtggttttg atgaaaatgg
      181 taataaagat ccatataaga ttacttttat atataaaaca ggaatacagg atgaaataaa
      241 agaagctaaa aaaaaatttg tagctaaagc atatgaacaa aaggaaaaag atacagaaat
      301 gtattctttt agtagtaacg accagaacaa tatgtgtaca aatgataatg gcaacatcaa
      361 tannnnnnnn nngccttcat gtgattccag taacatttct gattccagga gtgtttttaa
      421 tgtgctgttt tatttctaca tcgataggaa catccatggg aaccattgca gcaatggcac
      481 caatcgcgat caatgtggca cagggagcac acttaaatgt tgcgatcgtt ggagcagcag
      541 tgatcggagg ttcttatttt ggagataacc tttccatgat ctctgataca acgatctcag
      601 cagcagaagg atgcggttct gagatgaaag ataagtttaa gatgaacttc tttatcgcat
      661 taccggctgc gatcgtggca atgatccttt attcagtggt cggaggtact ggaagtggag
      721 caatcaaggc aggaagttac aatatcattg aagtccttcc atatcttatc gttcttggag
      781 cagcattgat ggggattaac gttgcagttg tactttttgt aggaatcctc tctgcaggta
      841 ttatcggact ttgtgtggga agctgcggat tctttgactg gattcaggca attggaaatg
      901 gtatgagtga tatgtttagt atcagtatgg ttgcgatctt ggtatctgga atcattggta
      961 ttgttcgcga atatggcgga gttgagtggc tggtaggagc gattagtggc aagatcaaga
     1021 atagacgtgg tgcagaatat ggaatcgggc tgatgtcagg aattctttct gcagcaatgg
     1081 taaataacac gatcgctatt attgttactt gtccgatcgc aaaagaaatc ggtgaaaaat
     1141 acaagattgc accaaagaga cttgccagtc tggtagatat ttttgcctgt gcatttatct
     1201 cattgatgcc acatgatgga ggtatgttaa tgatcacggc actcgcagat gtgtcaccac
     1261 ttaatgtgtt aaaatattca ttttatatgt ttggattgat cattgctact tgtgtgacga
     1321 tacagtttgg attgatggat gagaagaatt aagttgaaaa tacggtaatt catcaagtat
     1381 aaatagagat gggagtacga aaaatgaatt tccatctcta tttttgtgtt tatttatacc
     1441 cgaaggaacg ttggttaaaa ttagattgtt ttaaacggaa atggtgttga ttatgtgatt
     1501 ttgggacttg aaaatcaatc taagatacat tatgcgatgc cattaaggca tatgatagga
     1561 gatgctttca gttatttgca ggaatataag gaactggaag ccaagaaaaa aaatataaga
     1621 attataagac aaaacatgaa ttctt
//
LOCUS       DAOMUE010000373         1643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_20694_length_1643_cov_2.666247, whole genome shotgun sequence.
ACCESSION   DAOMUE010000373 DAOMUE010000000
VERSION     DAOMUE010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20694_length_1643_cov_2.666247"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PLC71_11165"
     CDS             <1..356
                     /locus_tag="PLC71_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03235.1"
                     /translation="QLLNIGGWMRQSAIVLVVLAVVILIIVFFVIFYKKARIEFISKI
                     QTIGFMKKIAWKRARTRFAGGMAMALKSGIDMDESLKEAVIKGAVQCYSVEGRYPESL
                     SYMEKHYGLEYDKDI"
     gene            353..808
                     /locus_tag="PLC71_11170"
     CDS             353..808
                     /locus_tag="PLC71_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4860 domain-containing protein"
                     /protein_id="HRM03236.1"
                     /translation="MTDQMFLVILFAVFAVCAFSLSMIGANIYSHTASVMNEDYEERI
                     DISYVTEKIRQWDEKDSIEIGSFHGRTALIHTEKINGKKYNTYLYQEKGALRELMVRE
                     GLDTTTMQGEKIVAAKDFSVIETKQLYHIKIQGSDGKIYQNCVYRHSRN"
     gene            817..1257
                     /locus_tag="PLC71_11175"
     CDS             817..1257
                     /locus_tag="PLC71_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03237.1"
                     /translation="MRQKSNGTHLILMELMMVLFFFAICGSILLQVFVKAHNISAKAR
                     EMTETKNYVTQAAELIEAGAYDIKDFQEYFTQIDGKAGDYQCYYDQDWNEIKKTKARY
                     HMTIKLERQPAKVLGKITMWRENQQIYQLDILRYRQERKDNERS"
     gene            1244..>1643
                     /locus_tag="PLC71_11180"
     CDS             1244..>1643
                     /locus_tag="PLC71_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03238.1"
                     /translation="MKDRKMPFLGIGAASIVLVLAMVCLAVFAALTLSSAKGDHTLSK
                     KNLERTSAFYQASNAANEQVGAIDEKLWKLYRRSKDKKDYMKRVGRSFTKSKGISYNK
                     KEKTIAFQESITDTQQLSVKLQIYYPEKKND"
BASE COUNT          589 a          222 c          402 g          430 t
ORIGIN      
        1 gtcagttatt gaatattgga ggttggatga ggcagtcagc aattgttctg gtagttttgg
       61 cagtggtgat cttgatcata gtatttttcg ttatttttta taaaaaggca agaattgaat
      121 ttatctcgaa gatacaaacc atcggattta tgaagaaaat cgcatggaaa cgcgcaagaa
      181 caagattcgc aggtggaatg gcaatggcat taaagagtgg aattgatatg gatgaaagtc
      241 tgaaagaagc cgtgatcaaa ggggcggtac aatgttacag tgtggaaggg cgatatccag
      301 aaagtctttc ttatatggag aaacattatg gattggagta tgacaaagac atatgacaga
      361 tcagatgttt cttgtgatct tatttgcagt gtttgcagtt tgtgcgtttt ccttgagtat
      421 gatcggggcg aatatatata gtcatacagc ttctgtgatg aatgaggatt atgaggaacg
      481 gatcgatatt tcttatgtaa cagaaaagat aagacagtgg gatgaaaagg acagcatcga
      541 gatcggcagc tttcatggac gcacagccct gatccataca gaaaagatca atgggaaaaa
      601 atacaataca tatctctatc aggaaaaggg tgcgttgaga gaattaatgg taagagaagg
      661 tctggataca acaacgatgc agggagagaa gatcgtggca gcaaaagatt tttctgtaat
      721 agaaacaaaa cagctgtatc atataaagat tcagggaagt gatggcaaga tatatcagaa
      781 ctgtgtatac agacacagca ggaattagga ggaagtatga gacaaaaaag caatggaaca
      841 cacctgatcc tgatggaatt aatgatggta ttattctttt ttgcaatctg tggatcaatc
      901 ctgcttcagg tatttgtaaa agctcataac atcagtgcaa aagcaagaga aatgacagag
      961 acaaaaaatt atgtaacaca agctgcagaa ctgatagaag cgggtgctta tgacatcaaa
     1021 gattttcaag aatattttac acaaatagat ggtaaagccg gagattatca gtgttattat
     1081 gatcaggatt ggaatgagat aaagaaaaca aaagcccgtt atcatatgac gataaagctt
     1141 gaaaggcagc cagcgaaagt tctgggaaag attacgatgt ggagggaaaa ccaacagatt
     1201 tatcagcttg atattttaag atacaggcag gaaaggaaag ataatgaaag atcgtaaaat
     1261 gccattttta gggattggag cagcttcgat cgtgctcgtg ttagctatgg tctgtttagc
     1321 agtttttgcg gctttgacac tttcaagtgc caagggagat catacgttaa gtaaaaagaa
     1381 tttggaacgt acgtcggctt tttatcaggc atccaatgca gcgaatgaac aagttggagc
     1441 gatcgatgag aaactatgga agttgtatcg cagatctaag gataaaaaag attatatgaa
     1501 aagggttgga cgatcgttta caaaaagcaa ggggatttca tataataaaa aagagaaaac
     1561 aattgcattt caggaaagta tcacagacac acagcagtta tctgtgaaat tgcagattta
     1621 ttatccggag aaaaagaatg atc
//
LOCUS       DAOMUE010000374         1643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_20698_length_1643_cov_2.432620, whole genome shotgun sequence.
ACCESSION   DAOMUE010000374 DAOMUE010000000
VERSION     DAOMUE010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20698_length_1643_cov_2.432620"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..>1643
                     /locus_tag="PLC71_11185"
     CDS             2..>1643
                     /locus_tag="PLC71_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRM03239.1"
                     /translation="MLYAGMIASLTMGIFISGAGQQKVEAKTKVTYTLKKGTLTIKGK
                     GAMPAKMKFRRNKKIKKVIIKKGVTSVSYEAFALCKNLNSVTIPSTVKTIGIRSFYGT
                     KISKITVPSKTKTIGQGAFGSCKDLKTIVMPGDFKLKLEEDTDDKLWYVTSDQSAVDT
                     ITFNTKLKLANVSYLSANNLVVAKNDPSYQSIEGVIYTKDGKGIVRVPQKRTELKIKE
                     GCTEFNMQSVLYNSTDSEGDEFNNCSKLKKIVIPSSVKSINKIKYKTDRADACDMHVD
                     TIEIAPKDFDANSLYALGSSLGKNITIESLMKLLPDQITYKDHMYITKDHGLLKYDGK
                     DANVEIPEEITWIAPEAFYRNETLKNVKLPSKITTIEENTFYGCSELEAVVIPDQVTM
                     IGKSAFDECTVLKSVTFGKSLKVIKDHAFASVNIRNFTIPSGIQKIETGAFAGINQIG
                     TVTFEGSTKYVATDAFMNSTGIKLVYKKGIKEAQTELSYDYIIARKNGNNKVRTTWQP
                     VSGANGYQLKFSTDKKFKKVLKTVMVKKNVSNATTYVKN"
BASE COUNT          643 a          233 c          338 g          429 t
ORIGIN      
        1 gatgttgtat gcaggcatga tcgcatctct gacaatgggg atttttataa gcggagcagg
       61 tcagcagaaa gtggaagcga aaactaaagt aacatacacg cttaagaagg gaacattgac
      121 gatcaaagga aaaggtgcaa tgcctgcgaa gatgaaattt cgaaggaaca agaagatcaa
      181 gaaagttatc attaaaaaag gcgtgacatc tgtttcatat gaagcgtttg cattatgtaa
      241 gaatttgaat tctgtgacaa ttccatcaac agtaaaaacg ataggaattc gaagcttcta
      301 tggaacaaag atttctaaaa taacggttcc atcaaagaca aagacgatcg ggcagggggc
      361 atttggaagt tgcaaagatt taaagacgat cgttatgcca ggagacttta aattaaaact
      421 cgaagaggat actgacgata agttatggta tgtcacaagt gatcagtcag cagttgatac
      481 cataacattt aatacaaaat taaaattggc aaatgtatca tatctgtcag caaataatct
      541 ggtagttgca aagaatgatc catcatatca gagtatcgag ggtgtaatct atacaaaaga
      601 tggaaaagga attgtaagag tacctcaaaa gagaacagag cttaagatca aagaaggatg
      661 tacagagttt aatatgcaat ctgtattata taattccaca gattctgaag gagatgagtt
      721 caacaattgt tctaaattaa agaagattgt aattccatca tctgtaaaga gtattaataa
      781 aataaaatat aaaacggatc gagcggatgc gtgtgatatg cacgttgata ccattgaaat
      841 tgctccaaaa gattttgatg cgaacagcct ttacgcatta ggaagttctc ttgggaagaa
      901 tatcacgatt gaatcattaa tgaaattact tccagatcag attacataca aagatcatat
      961 gtatattaca aaagatcacg gtttattaaa atatgacgga aaagatgcaa atgttgaaat
     1021 tccagaagag atcacatgga tcgcaccaga agcattttat agaaatgaaa cattaaagaa
     1081 tgtcaaactt ccatctaaga tcactacgat cgaagaaaat acattttatg gatgtagtga
     1141 attggaagcc gtggtaattc cagatcaggt aaccatgatc ggcaagtcag catttgatga
     1201 atgtacggta ttaaaatccg taacatttgg aaaatcctta aaagtgatca aagatcatgc
     1261 gtttgcttca gtaaatatca gaaactttac aatcccatca ggaattcaaa agatagagac
     1321 aggtgctttt gctggaatca atcagattgg gacggttaca tttgagggaa gtacaaagta
     1381 tgttgcaacg gatgcattta tgaattccac aggaataaaa cttgtatata agaaagggat
     1441 caaggaagcg caaacagaat tatcttatga ctatattata gccagaaaga atggaaataa
     1501 taaagttcgt acaacgtggc agccagtatc aggagctaat ggttatcagt taaaattcag
     1561 tactgacaag aaatttaaaa aagtattgaa aacagtaatg gtcaagaaaa atgtgtcaaa
     1621 tgctacaaca tatgtaaaga ata
//
LOCUS       DAOMUE010000375         1631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_20918_length_1631_cov_1.480330, whole genome shotgun sequence.
ACCESSION   DAOMUE010000375 DAOMUE010000000
VERSION     DAOMUE010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20918_length_1631_cov_1.480330"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /gene="citD"
                     /locus_tag="PLC71_11190"
     CDS             <1..268
                     /gene="citD"
                     /locus_tag="PLC71_11190"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HRM03240.1"
                     /translation="SSDAMVTVMPASQGEGIEFELDSVVIHQFGRQIKKVVMETLDRL
                     DVKDIKIKVVDKGALDCTLKARVECAVYRSCGQTDHLPWGGAIK"
     gene            268..1167
                     /locus_tag="PLC71_11195"
     CDS             268..1167
                     /locus_tag="PLC71_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008751353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRM03241.1"
                     /translation="MNPNKKRLRRTMMFLNCQKPGLIKDPYVYKPDSIMLDLEDAVAE
                     GEKDAARYSLYHALQEIDYRGVEKVVRINGLDTAYWEEDIRVCVAGGADTIRIPKCES
                     AEDIHKVEAKIEEAEEEFGREKGSTLIMAAIESAMGVMNVLEICQASDRLFGIALSGG
                     DYTKDLQTTISGTGVELAGARQQLIIAARAAGVQCFDTVFTNLDDMEAFEEEVKMIKQ
                     MGFDGKSIVNPRQIPIVHEIYTPTEKEIIFAEKVVKEIDEKKAQGIGVFTVDGKMIDI
                     AFYDGAKRTIELAKASGVYKGDL"
     gene            1169..>1631
                     /locus_tag="PLC71_11200"
     CDS             1169..>1631
                     /locus_tag="PLC71_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HRM03242.1"
                     /translation="MLNKVGREIPEEIIEATGKEVFQGIYYKDNTEFHKQGPITKVVM
                     NHDTSKMVDSIHDALVKCGAHDGMTLGFHHHFRDGDLVVNMVMKEVQKMGIKDVTICA
                     SSLGKAHDDLVPLIEDGTITNIQSSGVRGKIGEAISHGKLKGLATMRSHGGR"
BASE COUNT          599 a          258 c          381 g          393 t
ORIGIN      
        1 atccagtgat gcaatggtca cagttatgcc ggcatcacag ggtgaaggaa ttgaatttga
       61 attagacagc gttgtcatcc atcagtttgg aagacagatc aagaaagtgg ttatggaaac
      121 acttgaccgc cttgatgtca aagatatcaa gattaaagtg gtagataaag gagccttaga
      181 ttgcacatta aaagcaagag ttgaatgtgc agtataccgt tcatgcggac agacagatca
      241 tttaccatgg ggaggtgcga ttaaataatg aatccaaaca agaaaagatt aagaagaaca
      301 atgatgttct taaactgtca gaaaccagga ttaattaaag atccatatgt atacaaacca
      361 gactctatca tgttagacct tgaagatgcc gttgcagaag gagaaaaaga tgcagcaaga
      421 tattctttat atcatgcact tcaggaaatt gactatcgtg gagtagagaa agttgtacgt
      481 atcaacggat tagacacagc atactgggaa gaagatatcc gtgtctgtgt tgcaggtgga
      541 gccgatacaa ttcgtattcc aaaatgtgag agcgccgaag atattcataa agtagaagca
      601 aagattgaag aagctgaaga agaattcgga agagaaaaag gaagcacact gatcatggca
      661 gcgatcgaat ctgcaatggg tgttatgaat gttcttgaaa tctgtcaggc atctgaccgc
      721 ttattcggaa tcgcattaag tggaggagat tatacaaaag accttcagac aacaatttct
      781 ggtacaggag ttgagcttgc aggtgcaaga cagcagctta ttattgctgc aagagctgca
      841 ggagttcagt gctttgatac agtatttaca aatcttgatg atatggaagc atttgaagaa
      901 gaagttaaga tgatcaaaca gatgggattt gatggaaaat ccatcgttaa cccaagacag
      961 attccaatcg tgcatgaaat ctatacacca acggaaaaag aaatcatttt cgctgaaaaa
     1021 gttgtaaaag aaatcgatga aaagaaagca cagggaattg gtgtatttac agtagacgga
     1081 aaaatgatcg atattgcctt ctatgacgga gcaaagagaa caattgaatt agcaaaagct
     1141 tcaggcgtat acaaggggga tttataaaat gttaaataaa gtaggaagag agatcccaga
     1201 agaaattatc gaagcaacag gaaaagaagt cttccaggga atttattaca aagacaatac
     1261 agaatttcat aaacagggac cgatcacaaa ggttgttatg aaccatgaca caagcaaaat
     1321 ggtagattcc atccatgatg cactcgtaaa atgcggagca cacgatggaa tgacattagg
     1381 attccaccat catttccgag atggagacct tgttgtaaac atggtcatga aagaagtaca
     1441 gaaaatggga atcaaagatg taacaatctg tgcaagttct cttggaaaag ctcatgatga
     1501 tctggtaccg ctgatcgaag atggaacaat cacaaatatc cagtcttctg gagttcgtgg
     1561 aaagatcgga gaagcaattt ctcatggaaa attaaaagga cttgcaacaa tgcgttctca
     1621 tggtggacgt a
//
LOCUS       DAOMUE010000376         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_21142_length_1616_cov_1.884049, whole genome shotgun sequence.
ACCESSION   DAOMUE010000376 DAOMUE010000000
VERSION     DAOMUE010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21142_length_1616_cov_1.884049"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..569
                     /locus_tag="PLC71_11205"
     CDS             45..569
                     /locus_tag="PLC71_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03243.1"
                     /translation="MIRKPYKTNKNISRLFYILMMIIFVWFIGIQILGPDEQFFDQSG
                     HSIIYNGTFTWKKSDGTKQNISVPGRYKVPAKQTMIITTTLPDDYNENVIAIRSSLQD
                     VRFYIDGKLRKEYNAKTLPAVIYSAILRLQMPERNFVWNLQHIRVTTLVLSIRFTVEI
                     KCRSGVIFLIITSL"
     gene            527..>1616
                     /locus_tag="PLC71_11210"
     CDS             527..>1616
                     /locus_tag="PLC71_11210"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRM03244.1"
                     /translation="MQIWSYIFNHNFSVTVIGSFIFFASIVTILFSIALGIVYKTKFN
                     MEYLGWCMLMGSVWMIGESKMRQILVPNASGLATSCFIMLMLCPLPISLYVNNLQKGK
                     YKKIFQSICFIALLNFIICTILHLTGVADYIETMPAAHAILIITFLAVILTFWIRYWN
                     HRSRSDCLLFFGLLITMLSVIFEAISVYYKVSVSGVFVGIAILILLFMNVIYTIHIIR
                     DIIKRQQQEELDKRKKNIEEMSLQLMQMLSTTIEAKDEYTKGHSHRVAEYSVLIAREL
                     GWNEKELSNLKNAAHLHDIGKIAIPDTILNKPSKLSEEEFSIIKEHTIIGANILKNIS
                     LIDHVQEIVRNHHEHDPYTKNQRKSRLFN"
BASE COUNT          502 a          289 c          286 g          539 t
ORIGIN      
        1 tttgatatac aatgcgatca acaaatggag gggggaaact atttttgatc agaaaaccat
       61 acaaaactaa taaaaatatt tccagactat tttatatttt gatgatgatc atctttgtct
      121 ggttcattgg gattcagatt ttaggacctg atgaacagtt ttttgaccaa tctggacatt
      181 ccatcatcta caatggtact tttacatgga agaaatccga tggcacaaaa caaaacattt
      241 ctgttcctgg acgttataaa gttccagcaa aacagacgat gatcattact actacacttc
      301 cagatgatta taacgagaat gtgatcgcga tccgttcttc ccttcaagat gtacgttttt
      361 acattgatgg aaaacttcga aaagagtaca atgcaaaaac tctgccagcc gttatatatt
      421 ctgcaatact tcgtctgcag atgccggaaa ggaactttgt ctggaactta caacatatac
      481 gagtaactac tctggtgttg tcaatacgat ttactgtgga gatcaaatgc agatctggag
      541 ttatattttt aatcataact tctctgtaac cgtgatcgga tcatttatct tttttgcatc
      601 gattgtcaca atcttattta gtatcgccct tggaattgtt tacaaaacca agtttaatat
      661 ggaatatctt ggatggtgta tgcttatggg ttctgtctgg atgatcggag aatcaaaaat
      721 gcggcagatc cttgttccaa atgcatctgg acttgcaact tcatgtttta ttatgctcat
      781 gttatgtccg ttaccgattt ccttatatgt caataatctt cagaaaggta aatataaaaa
      841 gatctttcaa tcgatttgtt ttattgcttt attgaatttt atcatctgta cgatcctgca
      901 tcttacaggc gttgcagatt atattgagac aatgccagca gcacacgcaa tccttattat
      961 tactttcctt gctgtcattc ttactttctg gatcagatat tggaatcatc ggagtcgaag
     1021 tgattgcctg ttgttctttg gactgcttat tacaatgctt tctgtgattt ttgaagcaat
     1081 ttctgtatat tacaaagtat ctgtttctgg cgtatttgtc ggtatcgcta ttttgatctt
     1141 gttgtttatg aatgtaatct atacgattca tatcatccgt gatatcatca aacgccagca
     1201 gcaggaagaa ctcgataaac gtaaaaaaaa tatagaggag atgtctctgc agttgatgca
     1261 aatgttatcc accacgatcg aggcaaaaga tgaatatacg aaaggacatt ctcatcgtgt
     1321 tgcagagtat tccgttttga tcgcacgtga acttggatgg aatgaaaaag aacttagtaa
     1381 tctaaaaaat gctgctcatc ttcatgatat cggcaaaatt gctattcctg ataccatttt
     1441 aaataaacca agcaaacttt ctgaggaaga attttctatt attaaggaac atacgatcat
     1501 tggtgcaaat atcttaaaga atatttctct gatcgatcac gtacaagaaa ttgtacgcaa
     1561 tcatcatgag cacgatccgt acacaaaaaa ccaaagaaaa tctcgacttt ttaact
//
LOCUS       DAOMUE010000377         1592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_21582_length_1592_cov_1.503578, whole genome shotgun sequence.
ACCESSION   DAOMUE010000377 DAOMUE010000000
VERSION     DAOMUE010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21582_length_1592_cov_1.503578"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1592)
                     /locus_tag="PLC71_11215"
     CDS             complement(<1..>1592)
                     /locus_tag="PLC71_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRM03245.1"
                     /translation="IITEQITPYWKGKTFHEDLAKALPRETKKLTYNGDKELTSRYIV
                     NETASFRSSLQWVHDYEIVLEKGFEGIKEEALERLQQLDSDSPVDQTEKRPFLEAQII
                     TSDAIILWAERHAKLAKELSAKQRDADRKKELKIIAEICERVPRYPARTFYEALQSQW
                     FTQMFSRIEQKTGTIISNGRMDQYLYPFYQKDIKEGRITDEQVQELFECMWVAMSEFV
                     DLYLSEAGGSFNEGYAHWEAVTIGGTTKKGYDATNELTYILLKSKREFPLNYPDLAAR
                     IHMGSPKRYLYEVAETIKDGTGFPKLINDDEVVPILLSKGASLKEAYDYSVSGCAECR
                     MPNRDTYTSPYINFAAALEMVLHNGKMKKYGDEIVGLETGNFEDFESFDEVLEAYLKQ
                     QKNLIRHAFIQQHEIIRLRGEHFATPLGSSLHKLCRESYKDLHQPFIPGGIDLGYFEF
                     IGYATVVDSLSAIKKLIFEEKRLTKKELLEAVSNDFKGYEAIRQLLLHAPSYGNDDSY
                     TDEIGQLLDLEAQKFTHKYGKE"
BASE COUNT          401 a          345 c          263 g          583 t
ORIGIN      
        1 aattctttgc catatttatg tgtaaatttc tgtgcttcca aatctaataa ctgtccgatt
       61 tcatcggtat agctgtcatc atttccataa gatggtgcat gaagtaataa ctgtctgatc
      121 gcttcatatc ctttgaaatc gttgctcact gcttctaaca actctttctt tgtcagacgt
      181 ttttcctcaa agatcagctt tttgatcgct gatagggaat ctacgacagt tgcatatcca
      241 ataaattcaa aatatccaag atcaatccct ccaggaataa atggctgatg cagatccttg
      301 taactttctc tgcaaagttt atgaagtgat gatccaagtg gtgtagcaaa atgttctcct
      361 cgaagtctga taatctcatg ttgctgaata aatgcatgtc ggatcaaatt tttctgttgt
      421 tttaagtaag cctccagcac ttcatcgaaa ctttcaaaat cttcaaaatt tcctgtttct
      481 aatcctacga tctcatctcc atattttttc attttcccgt tgtgtaacac catttcaagt
      541 gctgctgcaa aattaatgta tggacttgtg taggtatctc tgtttggcat acggcattct
      601 gcgcaaccgg aaacgctgta atcataggct tcttttaaac ttgccccttt ggataataag
      661 atcggtacta cttcatcatc attaataagc tttggaaatc cagttccatc tttgatcgtt
      721 tctgctactt catagagata tctcttcggg cttcccatat ggatccttgc cgcaaggtcg
      781 ggataattta gcggaaattc tcgtttggac tttaataaga tataagttaa ttcattcgtt
      841 gcatcgtatc ccttctttgt cgttccgccg attgtcacag cttcccagtg agcatatcct
      901 tcattgaaag atccacctgc ctctgataaa tacagatcaa caaattcgga cattgctacc
      961 cacatgcatt caaataattc ctgtacttgt tcatccgtga tccttccttc tttgatgtct
     1021 ttttggtaaa atggatacag atattgatcc attcggccat tggatatgat cgttcctgtc
     1081 ttttgctcaa ttcttgaaaa catctgtgta aaccattggg attgcaatgc ttcataaaaa
     1141 gttcttgccg gatatcttgg aactctttcg cagatttctg ctatgatctt aagctctttt
     1201 ttcctatccg catccctttg ttttgctgat aattcttttg caagttttgc atgtcgttct
     1261 gcccataaga taatcgcatc acttgttatg atctgtgctt ctaggaatgg tcgtttctct
     1321 gtctgatcca ccggactatc tgaatctaat tgctgtaatc tttctaaggc ttcttcttta
     1381 attccctcaa atcctttctc gagaacaatt tcgtaatcat gtacccattg cagtgatgat
     1441 cgaaaagatg ctgtttcatt gacaatatat cttgaagtca actctttatc tccattataa
     1501 gtaagttttt ttgtttccct cggaagtgct tttgcaagat cctcatgaaa cgtcttccct
     1561 ttccagtaag gtgtgatctg ctctgtaatg at
//
LOCUS       DAOMUE010000378         1580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_21782_length_1580_cov_2.411148, whole genome shotgun sequence.
ACCESSION   DAOMUE010000378 DAOMUE010000000
VERSION     DAOMUE010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21782_length_1580_cov_2.411148"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..947
                     /locus_tag="PLC71_11220"
     CDS             102..947
                     /locus_tag="PLC71_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM03246.1"
                     /translation="MNVKIIRVLNTNAVVSVDSQGMELIMTGPGIGFKKRKGENIDQS
                     LVDKTYHLENKEESKRLQEVVKEISEEYLGIVDRVVKEAKKEKLEIRDSLYVTLTDHI
                     NSAVARYREGIALKNMMKIDIRKFYPKEYQVGMHAIEWIKEQTGEDLGDDEAAFIAMH
                     IVSAELNAQNITDVNQITELINTVLQIVRIHFKIDLNEESISYERFLTHLKFFAARVF
                     DHMEYKDTMQEIYKVMVEQNENAFSGVKKIAEYIKKQYNYKLTIDEQLYLLIHIKRIL
                     DEQTK"
     gene            1089..>1580
                     /locus_tag="PLC71_11225"
     CDS             1089..>1580
                     /locus_tag="PLC71_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HRM03247.1"
                     /translation="MDYENTAKKILQRVGGKDNVINLVHCMTRLRFTLKDESIVDDEA
                     VKKTKGVMGIMKKGGQYQIIIGNDVGNVFNELNKLGNFSNEVKEVPAKSNEKKNIFTM
                     LMDTISGIMAPVIPAIIGAAMIKVLLTLLPMIGVLSTNGQTYQLLSVIGDGAFFFMPV
                     LIAI"
BASE COUNT          627 a          188 c          321 g          444 t
ORIGIN      
        1 gacccgccgc aggcggagaa tcctgtaaag caggattctt tcttgtacat ttatagagat
       61 acaaaaaatg atgaaaaact tagataaaat tagaggagag gatgaacgtg aaaataatca
      121 gagttctgaa tacaaatgct gttgtcagtg tagattcaca aggaatggaa ttgatcatga
      181 caggtccggg aattggattt aaaaaaagaa aaggtgaaaa tattgatcag tctctggtag
      241 ataagacata tcatctagag aataaagaag aaagcaagag actacaggaa gtagtaaaag
      301 agatttcgga agaatacctt ggaattgtgg atcgtgtagt aaaagaggca aaaaaagaaa
      361 aattagaaat aagagattcc ttatatgtga cactgacgga tcatatcaat tcagcagtcg
      421 ctcgttatcg agaaggaatc gcattaaaga atatgatgaa aatcgatatt cgtaagtttt
      481 atccaaagga atatcaggtt ggtatgcatg cgattgaatg gattaaagaa cagacaggag
      541 aggatttggg agatgatgaa gctgcattta ttgcaatgca tatcgtgtct gcagaattaa
      601 atgcacagaa tataaccgat gtgaatcaga ttacagagtt gatcaataca gtcttacaga
      661 ttgtaaggat tcattttaag atagatttaa atgaagaatc tatttcgtat gagagatttt
      721 taacacattt aaagtttttt gcggcaagag tctttgacca tatggaatat aaagatacga
      781 tgcaagaaat ttataaagtg atggtagaac agaatgaaaa tgctttttca ggagtaaaaa
      841 aaattgcaga atatattaaa aagcaatata actataaatt aacaattgat gaacaattat
      901 atttattaat tcatatcaaa cgaattttag atgaacaaac aaaataatca taggtttgtt
      961 attattaagt taagcaagac ctgacaatag atgaaataac cccgggaatt attttatcta
     1021 ttgtcaggtc tttgttatat aaagaaaaat gaaaattttt aaatccagaa aaaaaggagg
     1081 aaaacaaaat ggattatgaa aatacagcaa aaaagatcct tcaaagagtt ggaggaaaag
     1141 ataatgtcat caatctggta cattgtatga caagacttcg tttcacatta aaagatgaat
     1201 ccatcgttga tgatgaagca gtaaagaaaa caaaaggtgt tatgggaatc atgaaaaagg
     1261 gcggacaata tcagatcatt attggaaatg atgttggaaa cgtatttaac gaattaaata
     1321 aacttgggaa tttttctaat gaagtaaaag aagtaccagc aaaatcgaat gaaaagaaaa
     1381 atatctttac aatgctgatg gatacgattt caggtatcat ggcacctgtt attccagcaa
     1441 tcattggtgc agctatgatc aaagtattgc ttacattact tcctatgatc ggtgtgttaa
     1501 gcacaaatgg gcaaacatat cagttattaa gtgttatagg agatggtgcg tttttcttca
     1561 tgccagtact gattgcaatt
//
LOCUS       DAOMUE010000379         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_21873_length_1574_cov_1.974984, whole genome shotgun sequence.
ACCESSION   DAOMUE010000379 DAOMUE010000000
VERSION     DAOMUE010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21873_length_1574_cov_1.974984"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PLC71_11230"
     CDS             complement(<1..644)
                     /locus_tag="PLC71_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM03248.1"
                     /translation="MSLEVKIKKKLKEFTLESELNVETGCTGLMGPSGSGKSITLKCI
                     AGVETADSGRIVLDGKILYDSEKKIDLPPQKRKIGYLFQGYALFPNMTIEENIKTGLR
                     AKGFPKDEIEQKTNDMMKRFHIEELAKRYPRQISGGQKQRTALARMLVCEPEVILMDE
                     PFSALDEYLREQMQDELSNMLAMLDKPAILVSHSSKEVENLCDKKYHIQRGHME"
     gene            complement(655..1329)
                     /gene="modB"
                     /locus_tag="PLC71_11235"
     CDS             complement(655..1329)
                     /gene="modB"
                     /locus_tag="PLC71_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HRM03249.1"
                     /translation="MDISPIIISMKTATIAILFTFILGLLAARWILYRKSDATRIFWD
                     GIFTMPLVLPPTVAGFFLLVFFGSYGPVGKLFENYLGIKIAFSWGATILAAVVMSFPL
                     MYRSARGALEQIDEDLIFAARTLGMSEWSIFFRVMIPNALPGIISGGVLAFARGLGEF
                     GATAMIAGNIPGRTRTLPLAVYSEVAAGNMDAAYQYVLVIVIICFAIVAAMNIYLIME
                     KKKQEA"
     gene            complement(1345..>1574)
                     /locus_tag="PLC71_11240"
     CDS             complement(1345..>1574)
                     /locus_tag="PLC71_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008423622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HRM03250.1"
                     /translation="DRNVPGISEAIRAGSMTITKRAMLSRGVSVIRKKTLIINLPGSK
                     KAVEESLSFIIDQLPHGLDTLRENTEDCGRN"
BASE COUNT          411 a          372 c          251 g          540 t
ORIGIN      
        1 ttttccatat gacctctttg aatatggtat tttttgtcac atagattttc tacttcctta
       61 ctgctgtggc tgaccaagat cgctggttta tccaacattg ccagcatatt ggataattca
      121 tcttgcatct gttctcttaa gtattcatcc aacgctgaaa atggctcatc catcaagatg
      181 acttccggtt cacacacaag catccttgct aacgctgttc gctgtttttg ccctcctgaa
      241 atctgtcttg gatatctttt tgctaattct tcgatatgaa atcgtttcat catatcattt
      301 gtcttttgtt cgatttcatc cttcggaaat ccttttgctc ttaatcctgt tttgatattc
      361 tcttcaatcg tcatattagg aaacagtgca tatccctgaa ataaatatcc aatctttcgt
      421 ttttgtggtg gcagatcgat ctttttctcg gaatcataaa gtattttccc atctagtaca
      481 attctcccac tatctgcagt ttccacacct gcgatacatt ttaaagtaat gctttttcca
      541 cttccagaag gtcccataag ccctgtacaa cctgtttcta cattgagttc tgactctaat
      601 gtaaattctt ttagtttctt tttgatcttt acttcaagac tcatacatct ctccttacgc
      661 ttcctgtttc ttcttttcca tgatcaggta aatattcatg gctgctacga ttgcaaaaca
      721 aatgatcacg atcacaagta catactgata tgctgcatcc atatttcccg ctgcaacttc
      781 agagtatact gcaagtggca gagttcttgt tcttcccgga atatttccag cgatcattgc
      841 tgttgctccg aattcaccaa gtcctctggc aaatgcaagc actccaccag aaatgatccc
      901 tggcagtgca tttgggatca taactcgaaa gaaaatgctc cactcactca ttccaagtgt
      961 tcttgccgca aagataagat cttcatcaat ctgttccagg gcacctcttg cagaacggta
     1021 catcaatgga aatgacatca caactgccgc aaggatcgtt gcgccccatg aaaatgcaat
     1081 cttgatccca agataatttt caaataattt tcctactggt ccatatgatc cgaaaaatac
     1141 aagcaagaaa aaccctgcaa cagttggtgg taatactaat ggcatcgtaa agatcccatc
     1201 ccagaagatt cttgttgcgt ctgatttacg atataagatc catcttgctg ctaatagtcc
     1261 taaaataaag gtaaataaaa tcgcgatcgt tgccgttttc atcgatatga tgatcggtga
     1321 tatatccata cattgtctcc tctcttagtt tcttccgcaa tcttctgtgt tttcccttaa
     1381 agtatctaat ccatgtggca gctgatcaat gataaaagaa agactctctt ccactgcctt
     1441 tttgcttcca ggaagattaa tgatcaatgt tttctttctt attacactga cacctcttga
     1501 taacatggct cgttttgtga tcgtcataga acctgctctg atcgcttctg agattcccgg
     1561 tacattgcgg tctg
//
LOCUS       DAOMUE010000380         1564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_22061_length_1564_cov_1.656064, whole genome shotgun sequence.
ACCESSION   DAOMUE010000380 DAOMUE010000000
VERSION     DAOMUE010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22061_length_1564_cov_1.656064"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PLC71_11245"
     CDS             <1..762
                     /locus_tag="PLC71_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HRM03251.1"
                     /translation="NLILATGGCAQPNLGSNGSGYKLAKDFHHKITDTFPSLVGLEAE
                     GKYLKDTSGVRNVSNVSVYANNELIAKEHGEVQFTKYGVSGIPIFQISHFAIEALRRK
                     KKVKIYLNLMPQMVTLEETTEVFLKSCNYKTVEEFFQGFLNKKLVYAILLRLKIDPRK
                     PVAQISRKDLNRILDMIQHFEVEIKGYKGYDMAQVTAGGVSTKEIIQGTLESKLLEGL
                     YVVGELLDVDGICGGYNLQWAFTSGYIAASQIGRQ"
     assembly_gap    861..870
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <871..>1076
                     /locus_tag="PLC71_11250"
     CDS             <871..>1076
                     /locus_tag="PLC71_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRM03252.1"
                     /translation="KIVKRSIDARKKEELSYVYAVDVTLGKGQEEKFLKKNKNRNIMA
                     VKEKKFEFPENQREFKYPPVVIGMG"
     assembly_gap    1077..1086
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1087..>1564
                     /locus_tag="PLC71_11255"
     CDS             <1087..>1564
                     /locus_tag="PLC71_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRM03253.1"
                     /translation="PVVYQIPGRKELLSEKREQMAVIGAGPAGLFAALMLARAGLHPI
                     VLERGKDVTNRMKDVEHFWESGELNPESNVQFGEGGAGTFSDGKLNTLVKDKFGRNRF
                     VLETFVEHGAPEEILYENKPHIGTDLLRNVVAGIREEIRSLGGDVRFEAKVTDFVIQ"
BASE COUNT          569 a          246 c          360 g          369 t
ORIGIN      
        1 aatctgatcc ttgcaactgg tggatgtgca cagccaaact taggaagcaa cggaagtgga
       61 tacaagcttg ccaaagactt tcatcataag atcacggata cattcccgtc tttggtagga
      121 ttagaagcag aaggaaaata tttaaaagac acctcgggag ttcgaaatgt cagtaatgtc
      181 agcgtctacg cgaataatga attgatcgcc aaagaacatg gagaagttca gtttacaaaa
      241 tatggggttt cagggatccc gattttccag atcagtcatt ttgcgatcga agcattaaga
      301 agaaagaaaa aagtaaaaat ctatttaaat ctcatgccac agatggtaac cttagaagaa
      361 acaacagaag tattcttaaa gagctgcaat tataagacag tcgaagaatt cttccaggga
      421 tttttaaata aaaaactggt atatgcaatc ttactgagat taaagattga tccaagaaaa
      481 ccagttgctc agatatccag gaaagatctt aacaggatct tagatatgat ccaacacttt
      541 gaagttgaga tcaaaggata taaaggatat gatatggcac aggtaacagc cggaggcgta
      601 tccacaaaag aaatcataca aggaacatta gaatcaaagt tattagaagg gctttatgtt
      661 gtcggtgaat tactagatgt cgatggaata tgcggagggt acaaccttca gtgggcattt
      721 acatctggat acatcgctgc aagccagata ggaagacaat gataagaatc aaccagatca
      781 agctgccgat cgatcacaaa gaggcagcac ttgagaagaa gataaaaaaa gcattacata
      841 atacagaata taaacaatac nnnnnnnnnn aagatcgtaa aacggtctat tgatgcgagg
      901 aaaaaagaag aattatctta tgtgtatgca gttgatgtga cattaggtaa gggtcaagaa
      961 gagaaattcc tcaaaaagaa caagaatcgt aatatcatgg cagtaaaaga aaagaagttt
     1021 gaatttccag aaaatcagcg ggaatttaaa tatccaccgg ttgtgatcgg aatgggnnnn
     1081 nnnnnnaccg gtggtttatc agatcccagg aaggaaagaa ctactttctg aaaagcgtga
     1141 gcaaatggct gtgatcggtg caggaccggc aggtctgttt gcagcactga tgcttgcaag
     1201 agccggatta catccgatcg tcttagaacg agggaaagat gtcacaaacc gtatgaaaga
     1261 tgtagaacat ttctgggaga gtggtgaact gaatcctgaa tccaatgtac aatttggaga
     1321 aggaggagca ggaacatttt cagacggaaa actaaatacc ttagtaaaag acaaattcgg
     1381 aaggaataga ttcgttttag aaacttttgt agaacatggt gcaccagaag agatcttata
     1441 tgaaaataaa ccacatatcg gaacggatct tttaagaaat gtcgttgcag ggatcagaga
     1501 agagatcaga tctcttggcg gagatgtccg ttttgaggcg aaagtgacag atttcgtgat
     1561 ccag
//
LOCUS       DAOMUE010000381         1544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_22375_length_1544_cov_2.435863, whole genome shotgun sequence.
ACCESSION   DAOMUE010000381 DAOMUE010000000
VERSION     DAOMUE010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22375_length_1544_cov_2.435863"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /gene="pheS"
                     /locus_tag="PLC71_11260"
     CDS             <1..175
                     /gene="pheS"
                     /locus_tag="PLC71_11260"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442524.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRM03254.1"
                     /translation="LGCGMVHPHVLEMCGIDPEVYSGFAFGIGLERVALLKYEIDDMR
                     LLYENDKRFLSQF"
     gene            232..>1544
                     /gene="pheT"
                     /locus_tag="PLC71_11265"
     CDS             232..>1544
                     /gene="pheT"
                     /locus_tag="PLC71_11265"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRM03255.1"
                     /translation="MDTPISWIKAYVPDLDCTPQEYADKMTLSGSNMESVTYLDKNLE
                     KIVVGKIEKIEKHPDADKLIICQVNVGDETVQIVTGAPNVSEGDLVPVVLDGGRVAGG
                     HDGGKNPEDGIKIKKGKLRGVESFGMMCSIEELGSSRDMYPEAPEYGIYIFNKDVKPG
                     DDAVEALGLRDAVVEFEITSNRVDCFSMIGMAREAAATFKKDFFPPVVTKTGNDEDVN
                     DYIKVRVEDEDLCPRYTARVVKNIKLAPSPEWMQRRLAAMGIRPINNLVDITNYVMEE
                     YGQPMHAYDLDTIANKEIVVRRAKAGDKFTTLDGEERTMDENVLMICDGEKEIGIAGI
                     MGGENSMVTDDIKTLLFEAACFDGTNIRLSSKRIGLRTDASGKFEKGLDPENAMAAMD
                     RACQLIEELGAGEVVGGAVDVYPNPVKLIRLPLEVDKMNALLGTNV"
BASE COUNT          553 a          264 c          365 g          362 t
ORIGIN      
        1 cttaggatgt ggaatggttc acccacacgt gcttgaaatg tgcggaatcg acccagaagt
       61 atattcagga tttgcatttg gaatcggttt agagcgtgtc gcattattaa aatacgagat
      121 cgacgatatg cgtctgttat acgaaaacga taagagattc ttatcacagt tctaagagaa
      181 acgataagaa tatttcgaat agagaagata gaataaagga gaataaaagt aatggataca
      241 ccaatttcat ggatcaaagc ttatgtgcct gatcttgatt gcacaccaca ggaatatgca
      301 gataaaatga cattatctgg ttccaacatg gaaagtgtca catatttaga taaaaactta
      361 gaaaagatcg tagtcggaaa gatcgagaag atcgaaaaac atccagacgc agacaaactg
      421 atcatctgtc aggtgaatgt tggagatgaa acagttcaga tcgtaacagg agcaccaaac
      481 gtatcagaag gagacttagt accagttgtc ttagacggtg gacgagttgc cggaggacat
      541 gacggtggaa agaacccaga agacggaatt aagattaaaa aaggaaaact tcgtggagta
      601 gaatcttttg gtatgatgtg ttctattgaa gaacttggaa gttccagaga catgtaccca
      661 gaagcaccgg aatatggaat ctacattttc aataaagacg taaaaccagg agatgatgca
      721 gttgaagctt taggacttcg cgatgcagtt gtcgaattcg agatcacatc aaaccgtgta
      781 gactgcttca gcatgatcgg tatggcaaga gaagcagccg ctacatttaa gaaagacttc
      841 ttcccaccag ttgtcacaaa gactggaaat gatgaagatg tcaatgatta catcaaagta
      901 agagtcgaag acgaggatct ttgcccaaga tacacagcaa gagttgtaaa aaatatcaaa
      961 ttagcaccat caccagaatg gatgcagaga agattagctg cgatgggaat ccgtccgatc
     1021 aataacctgg ttgacatcac aaactacgtg atggaagaat acggacagcc aatgcatgca
     1081 tacgatcttg atacgatcgc aaacaaagag atcgtcgtac gccgtgcaaa agcaggagat
     1141 aaattcacta cattagatgg tgaagaaaga acaatggatg aaaatgtctt aatgatctgt
     1201 gatggagaaa aagaaatagg catcgcaggt atcatgggtg gagaaaactc catggtaaca
     1261 gacgatatca agacattatt atttgaagca gcatgctttg atggaacaaa tatccgactt
     1321 tcttctaaga gaatcggttt aagaaccgat gcatctggta aattcgaaaa aggattagac
     1381 ccagaaaatg caatggcagc gatggatcgt gcatgccagc tgatcgaaga attaggagca
     1441 ggagaagttg ttggaggagc cgttgatgtt tatccaaatc cagtcaaact gatccgcctt
     1501 ccattagaag tagacaagat gaatgccctt cttggaacaa atgt
//
LOCUS       DAOMUE010000382         1538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_22486_length_1538_cov_2.525287, whole genome shotgun sequence.
ACCESSION   DAOMUE010000382 DAOMUE010000000
VERSION     DAOMUE010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22486_length_1538_cov_2.525287"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1316)
                     /gene="thiH"
                     /locus_tag="PLC71_11270"
     CDS             complement(150..1316)
                     /gene="thiH"
                     /locus_tag="PLC71_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HRM03256.1"
                     /translation="MSVRIDENTDHMKYTDDMEAIDSDIMDRVIAEMNSYDADIYTAK
                     DVKEALAAETCSVDNFKALLSPAALPFLEEIAQKAQKETRKHFGNSVAIFTPLYIANY
                     CENYCVYCGFNCHNKIKRAQLNAEEIEKEMQAIAETGLEEVLILTGESPNKSSVEYIG
                     EACKIAKKYFKLIGLEVYPMDSKDYAYLHECGADFVTVFQETYNSDKYKTLHLGGRKR
                     IFPYRLNAQERAIMGGMRGVGFAALLGLDDFRKDALATGMHAYLLQKKYPHAEIAFSC
                     PRLRPIINNDKINPKDVHEPQLLQIICAYRIFMPFASITISTRECERFRDNIIQIAAT
                     KISAGVNVGIGGHSQEEEKGDEQFEISDGRSVDEIYQMIEDNGMQPVMTDYIYV"
     gene            complement(1319..>1538)
                     /locus_tag="PLC71_11275"
     CDS             complement(1319..>1538)
                     /locus_tag="PLC71_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRM03257.1"
                     /translation="IGKPSQACEAMEMGAAAVMANTAIATAGDIPAMAEAFKKAIEAG
                     RSAYLAGLGRVIEKGSSASSPLTGFLED"
BASE COUNT          390 a          346 c          252 g          550 t
ORIGIN      
        1 aaaaatcctt gatctttctc tgtattcttc tttttagaag cggtgagctt tttattgcat
       61 aggggattcc atcggatttt cctaggataa aaatcccgga tcttttattt tgatccggga
      121 ttgtgtatac tatatacttt tttgttttct tatacataaa tataatctgt cataactggc
      181 tgcatgccat tgtcctcgat catttgataa atctcatcaa ctgatcttcc atcactgatc
      241 tcgaactgtt catcaccttt ttcttcttcc tgactgtgtc caccgattcc aacatttact
      301 cctgctgaaa tcttcgtcgc tgcgatctga atgatgttgt cacggaatct ttcacattca
      361 cgtgttgaga tcgtaatact tgcaaatggc ataaagattc gatatgcaca aataatctgt
      421 agaagctgtg gttcatgaac atcttttgga ttgatcttat cgttattaat gatcggacgt
      481 aatctaggac atgaaaatgc gatttctgca tgtggatatt ttttctgtaa aagatatgca
      541 tgcattcctg ttgccagtgc atcttttcta aaatcatcca atcctaagag agcagcaaat
      601 ccgacacctc tcattccacc catgatcgct cgttcctgag cattgagacg gtatgggaat
      661 attctctttc ttccacccaa atgcagtgtc ttatatttgt ctgaattata tgtttcctgg
      721 aagactgtaa caaaatctgc cccacattca tgcagatatg cataatcttt agaatccatt
      781 ggatatactt caagaccgat cagtttaaaa tatttctttg cgatcttaca tgcttccccg
      841 atatactcaa cacttgattt gtttggactt tctccagtca ggatcaaaac ttcttccaat
      901 cctgtctctg cgatcgcctg catttctttt tcaatctctt ctgcattaag ctgtgcacgt
      961 ttgatcttgt tgtggcaatt aaatccacag taaacacaat aattttcaca gtagtttgca
     1021 atatataacg gagtaaagat cgctacagag tttccaaaat gttttcttgt ctctttctgt
     1081 gctttctgtg cgatttcttc caaaaacggc aatgccgctg gcgataataa ggctttgaaa
     1141 ttatcaacag aacaagtttc tgctgccaat gcttctttga catcttttgc tgtataaatg
     1201 tctgcatcat agctgttcat ctctgcgatg acacggtcca tgatatctga atcaatcgct
     1261 tccatgtcat ccgtatattt catatgatct gtattctcat caattcttac actcatgatt
     1321 aatcctccaa aaatcctgtt aatggtgatg atgcgctaga acctttttca atgactcttc
     1381 caagtcctgc cagatatgcg cttcttcctg cctcaattgc tttcttaaat gcttctgcca
     1441 tggctggaat atcacctgca gttgcgattg ctgtattcgc catcactgca gctgcaccca
     1501 tttccattgc ttcacatgcc tgagatggtt ttccgatt
//
LOCUS       DAOMUE010000383         1525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_22752_length_1525_cov_2.157143, whole genome shotgun sequence.
ACCESSION   DAOMUE010000383 DAOMUE010000000
VERSION     DAOMUE010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22752_length_1525_cov_2.157143"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PLC71_11280"
     CDS             <1..660
                     /locus_tag="PLC71_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRM03258.1"
                     /translation="YAKQMGVPIDKLVCASNDNKVLFDFFQTGDYDRNREFILTTSPS
                     MDILISSNLERLLYRISGDNDQKTKDMMEALKSTGKYTITEDMKARLDDFAAGYATEE
                     ECAQNIKKVYDKTGYVMDTHTSVASYVCECYQKNSGDNKKCVIASTASPYKFVKSVMS
                     AIDPKYADQDEFSLLKVLEETSGREMPQAIKDILNANILHDLECDADKMKDTVKNILE
                     V"
     gene            945..>1525
                     /locus_tag="PLC71_11285"
     CDS             945..>1525
                     /locus_tag="PLC71_11285"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRM03259.1"
                     /translation="MAKIDLSKYGISGTTEIVHNPSYELLFEEETKPELEGFEKGQES
                     ELGAVNVMTGIYTGRSPKDKFIVVDENSKDTVWWTSDEYKNDNHPATQEAWKAVKDIA
                     IKELSDKKLYVVDAFCGANKDTRMAIRFIVEVAWQAHFVTNMFIKPSEEELENFEPDF
                     VVYNASKAKVENYKELGLNSETAVMFNITSKEQV"
BASE COUNT          589 a          222 c          324 g          390 t
ORIGIN      
        1 tatgcaaaac agatgggagt cccaatcgat aaactggtat gtgcatcgaa tgacaataaa
       61 gtgttatttg acttcttcca gacaggtgat tatgacagaa accgtgagtt tatcctcaca
      121 acatcaccat ctatggatat cctgatttca agcaacctgg aacgtctgct ttatcggatc
      181 agcggagata atgaccagaa gacaaaagat atgatggaag cattaaaatc cacaggaaaa
      241 tatacaatta cagaagatat gaaggcacgt cttgatgatt tcgcagctgg atatgcgaca
      301 gaagaagagt gtgcgcagaa tatcaagaaa gtatatgata agacagggta tgtgatggat
      361 acacatacaa gtgtagcatc ttatgtgtgt gaatgttatc agaagaacag tggagacaat
      421 aagaaatgcg tgattgcatc aacagcaagt ccatataaat tcgtgaagag tgtcatgagt
      481 gcaattgatc cgaagtatgc agatcaggat gagttcagtc ttctgaaggt tctggaagaa
      541 acatcaggac gtgagatgcc gcaggcgatc aaagatatcc tgaatgcaaa tattctgcat
      601 gatctggaat gtgatgctga taagatgaaa gatacagtga agaatattct ggaagtatag
      661 aacgtttaca aaatgataaa tatattttct gtattgataa aaatacataa aatgcaagat
      721 ttcgtgtgaa atcttgcatt ttgtgctttt aaaacaaaaa atagaataaa aaataaaagt
      781 tttagagaaa gaacttgcaa aaacacattg tgaaaagatt gttaaagtat tgacttttgc
      841 gtagaaaaat gcaataataa aacaaatgct gttgtgggag aatataacaa gactagaatt
      901 tataaatata cagcagcaaa taaaagaaaa gaggttaaat gaagatggct aaaatcgatt
      961 taagcaagta tggcatttca ggaacgacag aaatcgtaca caatccttct tatgaattat
     1021 tatttgagga agagacaaaa ccagaactcg aaggttttga aaaaggacag gaaagtgaac
     1081 ttggtgcagt caatgtaatg acaggtatct acactggacg ttctccaaaa gataagttca
     1141 tcgtagttga tgagaattca aaagatactg tatggtggac atctgatgaa tataagaatg
     1201 ataaccatcc tgcaacacag gaagcttgga aagcagtaaa agatattgcg atcaaagaac
     1261 tttctgacaa aaaactttat gttgtagatg cattctgcgg agcaaacaaa gatacacgta
     1321 tggcaatccg tttcatcgta gaagttgcat ggcaggctca tttcgtaaca aatatgttca
     1381 tcaaaccttc agaagaagag cttgaaaact ttgagccaga cttcgtagta tacaatgctt
     1441 ctaaagcaaa agttgaaaat tacaaagaat taggactgaa ttcagagaca gcagtaatgt
     1501 tcaacatcac aagcaaagag caggt
//
LOCUS       DAOMUE010000384         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_23210_length_1501_cov_2.919779, whole genome shotgun sequence.
ACCESSION   DAOMUE010000384 DAOMUE010000000
VERSION     DAOMUE010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23210_length_1501_cov_2.919779"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLC71_11290"
     CDS             <1..732
                     /locus_tag="PLC71_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HRM03260.1"
                     /translation="MKNLNAIDDQMADELMYVVDTCENDPNVKVVVLKGMPKAFSAGG
                     DIGYFYNLIQQGGEVNMDSLISKVGNVTDGLKRMSKLVISSVSGAAAGAGVSLAFSGD
                     FVVCADNAKFIMAFVNLGLVPDTGGTYLFVKSLGIQKAMELCVTGRPMKAQEAKDAGM
                     VYDVVPAEELDDTVMKLAKKFASGPLLSYKNIKKQIYQAGLADYKKYLDECEIPTQRE
                     CAASSDFIEGVKAFMEKRKAEFKGE"
     gene            774..1490
                     /locus_tag="PLC71_11295"
     CDS             774..1490
                     /locus_tag="PLC71_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HRM03261.1"
                     /translation="MKSKVVTREEALSRIHDGQTIMFGDWHGEFAADEIIDGMLEKGI
                     KDIDAIAVSAGMADQGVGKLIKDHRVKSLITTHIGLNPIAKDQMFAGELAIEFSPQGT
                     FAERIRCGGAGLGGCLTPTGLGTEVEEGKQKLTIDGKEYLLELPLRADVALIKATKAD
                     TAGNISFRMNSRATNSTMAMAADTVIVEVEEIVEVGELGPEEIDVPAPVIDMIYERTG
                     EKRPMCPMWQRAKAKAEGGQ"
BASE COUNT          497 a          232 c          407 g          365 t
ORIGIN      
        1 atgaagaatc ttaatgcaat tgatgatcag atggcagacg agttgatgta cgtagtagat
       61 acttgtgaga atgacccgaa tgtaaaagtt gttgttttaa aaggaatgcc aaaggcattt
      121 tcagcaggag gagacattgg atacttctat aacctgatcc aacaaggtgg agaagtgaat
      181 atggattctc tgatcagtaa agttggcaat gtaaccgatg gtctgaagcg tatgagtaag
      241 cttgtgatct caagtgtaag tggagcggca gcaggtgcag gagtaagtct tgcattttca
      301 ggggactttg ttgtatgcgc agacaatgcg aagtttatta tggcatttgt gaatcttgga
      361 ctagttccag ataccggtgg aacgtacctg tttgttaaga gtcttggaat tcagaaggca
      421 atggaactgt gtgttacagg aagaccaatg aaagcacagg aagcaaaaga tgccggaatg
      481 gtatatgatg tagttccagc ggaggaatta gatgataccg ttatgaaact tgctaagaaa
      541 tttgctagtg gaccattact ttcttataag aatatcaaga aacagattta tcaggcaggg
      601 cttgctgatt ataagaaata cctggatgaa tgtgagattc cgacacagcg tgaatgtgcg
      661 gcatcttctg attttataga aggagtcaaa gcttttatgg agaagagaaa ggctgaattc
      721 aaaggagaat aaagccagga tcaaaccatg aacatagaag ggaggtcacg cctttgaagt
      781 caaaagttgt aacaagagag gaagcgctct caagaattca tgatggacag acgatcatgt
      841 ttggagactg gcatggagaa tttgcagcag atgagatcat tgatggaatg ttagaaaaag
      901 gaatcaaaga cattgatgcg atcgctgtat ctgctggaat ggcagatcaa ggagtcggaa
      961 agctgatcaa agatcacaga gttaagagtt tgatcacaac acatattggg ttgaacccga
     1021 tcgccaagga tcagatgttt gcaggagaac ttgcgataga atttagtccg caaggaacct
     1081 ttgcagaaag aatccgttgt ggtggagcag gcttaggagg ctgtctgaca ccaacaggac
     1141 ttggtacaga agtagaagaa ggtaaacaaa aactaacgat cgatggcaaa gaatatcttc
     1201 tggaattacc attgcgtgca gatgttgctt taatcaaagc aacgaaagca gatactgcag
     1261 gtaatatttc attccgaatg aattccagag caacaaacag tacgatggct atggctgctg
     1321 atacggtgat cgttgaagtc gaagagatcg tggaagtggg tgaactagga ccagaagaga
     1381 tcgatgttcc agcacctgtg atcgatatga tctatgaaag aaccggtgag aaacgaccaa
     1441 tgtgtccaat gtggcagcga gccaaagcaa aagcggaagg aggtcagtga tatggcagga
     1501 t
//
LOCUS       DAOMUE010000385         1500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_23237_length_1500_cov_2.917647, whole genome shotgun sequence.
ACCESSION   DAOMUE010000385 DAOMUE010000000
VERSION     DAOMUE010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23237_length_1500_cov_2.917647"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /gene="ribD"
                     /locus_tag="PLC71_11300"
     CDS             complement(<1..635)
                     /gene="ribD"
                     /locus_tag="PLC71_11300"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRM03262.1"
                     /translation="MRRAIELAKKGCGYTNPNPLVGAVIVKDQRVIGEGYHEKIGGLH
                     AERNALKNCIEDPKGAEIYVTLEPCCHYGKTPPCTEALIEAGIKKVYVGNLDPNPKVA
                     GGGIKILNDHGIETETGILEEECRQLNDIFFHYIQNDIPYTALKYAMTLDGKIATATG
                     ESKWITGEEARRHVHTLRHQYAAIMAGIGTVLADDPMLNARIEHGNDPIRV"
     regulatory      complement(856..973)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1049..>1500)
                     /locus_tag="PLC71_11305"
     CDS             complement(1049..>1500)
                     /locus_tag="PLC71_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03263.1"
                     /translation="GYPPTRAGAEQAYQDYMDGKIDVPEADAYMGKNQKNDIEEKKNS
                     TVKTETKKDKQSKNTNKNSTTKQENSSTEQTNNSTTSAKESTTQGTTETTTTKVTTET
                     KEQKQQKEEKKKKEQKETKKKHTTGIAIGAAAVVIFALLVVFMRKRM"
BASE COUNT          325 a          327 c          245 g          603 t
ORIGIN      
        1 atcacacgaa ttggatcatt cccatgttcg attcgtgcat ttaacatcgg atcatctgct
       61 aaaaccgtac cgatccctgc catgatcgct gcatactgat gtcgtaatgt atgtacatgt
      121 cttctggctt cttctcctgt gatccacttg gattcaccag ttgccgttgc gatctttcca
      181 tccaatgtca ttgcgtattt tagtgctgta tatggaatat cattctgaat ataatgaaaa
      241 aatatatcgt ttaactgtct gcactcttct tcaagaattc ctgtctcagt ttctattcca
      301 tgatcattca ggatcttgat cccgccccct gctactttcg gattgggatc taaattcccg
      361 acatatactt tcttgatccc tgcttcgatc aatgcttccg tacatggtgg tgtttttcca
      421 taatggcagc atggctccaa tgttacataa atctcagctc ccttaggatc ttctatgcag
      481 ttttttaagg catttcgctc tgcatgtaat ccgccgatct tctcatgata tccttcccca
      541 ataacccttt gatcttttac gatcactgct ccgactaaag gatttggatt ggtatatcca
      601 cagcctttct tggcaagttc gatcgcacgc cgcatatatt cttctttcaa atatttttct
      661 cccttccctt caggttcttc gattttggga ggttggattt tgattggtat tgaaatcgga
      721 aatataaaaa ggagctcctg atctttgttc taattcttca ggaactcctt tttgatatct
      781 tgatatttct tttaattgaa ataaaaaaat cctggaatta taaaaatccc aggacatgat
      841 gatcaatatc acttcttaaa tcttcttcca tccagactgt actgtcggcc ttggaatctc
      901 accaagtcat gccatataag gctcgcgggc tataaccgcc ggtagggact tgcaccctgc
      961 cctgaagacg ttatgtaatt tacatataga atagcactca ctaactgttg tgtcaagtga
     1021 attttctatc acttcagcat agcatttttt acattctctt tcgcataaat actacaagta
     1081 aagcaaaaat cacaactgca gctgctccga ttgcaattcc tgttgtatgt tttttctttg
     1141 tttctttctg ctcttttttc tttttttctt ctttttgttg tttctgttct tttgtttctg
     1201 ttgtcacttt ggtcgttgtt gtttctgttg ttccttgtgt tgttgattct tttgcagatg
     1261 ttgtactgtt gtttgtctgt tctgtagatg aattttcttg ttttgttgta ctatttttat
     1321 tggtattttt agattgttta tctttttttg tttctgtctt cacagttgaa tttttctttt
     1381 cttctatgtc atttttctgg ttctttccca tatatgcatc tgcttctgga acatcaatct
     1441 ttccatccat ataatcctga tatgcctgtt ctgcccctgc tcttgttggt ggataaccat
//
LOCUS       DAOMUE010000386         1445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_24363_length_1445_cov_2.811511, whole genome shotgun sequence.
ACCESSION   DAOMUE010000386 DAOMUE010000000
VERSION     DAOMUE010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24363_length_1445_cov_2.811511"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PLC71_11310"
     CDS             complement(<1..705)
                     /locus_tag="PLC71_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391963.1"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HRM03264.1"
                     /translation="MAMVEKIKNKLRGNGFLLAVTIVLFFVLYIAGAILYGDRNFTSP
                     QVFLNLFISNAGLIIAAVGMTFVLITGGIDISIGSVIGVVCMMLAWMMEVKHMSAVVA
                     IILVLAFGVIFGLVQGFLVAYLKIQPFIVTLGGMFFARGLTALISSDMISIENPTFLS
                     LANYRIYFGFITYYNNRGVAMHPYIYLSVVIALLVVAISFVILRYTKFGRAVFAVGGN
                     EQSALLMGLNPKVTKLK"
     gene            complement(705..>1445)
                     /locus_tag="PLC71_11315"
     CDS             complement(705..>1445)
                     /locus_tag="PLC71_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM03265.1"
                     /translation="IPWIAAILIGLVVSVLCGMWNGFLVANMKIQPMVATLILFTAGR
                     GMAQLITGGNNLYVKVDSFKYLGGFLPHIPVPTPILVAIIVVILATLFLNKTATKLYI
                     ESVGINPRASRLFGLNSTKIIFITYAACGLCAGIAGMIATSRITSIDANNVGNLMELD
                     AILAVALGGNSLAGGKFSVAGSVIGAITIQALSTTLYAMKVTGDQLPLYKAIVVIIIV
                     TLQSPEFKKYLNRIKAKRAAKTAGEKVA"
BASE COUNT          452 a          314 c          244 g          435 t
ORIGIN      
        1 ttttaatttt gtaactttag gatttaatcc cattaataat gcagactgtt catttccacc
       61 tactgcaaat acggcacgtc caaattttgt gtatcgaagg atcacaaagg aaatcgctac
      121 aaccaataat gcaatgacaa cacttaggta gatataagga tgcattgcaa ctcctcggtt
      181 gttgtaatat gtaataaatc caaaataaat tcgatagttt gcaagactta aaaatgttgg
      241 attttcaata ctgatcatat cggaactgat caatgctgta agacctctgg caaagaacat
      301 tcctccaagt gttacgatga atggctgtat ttttaaataa gctactaaaa atccttgaac
      361 caatccgaag atcacaccaa atgctaatac cagaataatt gcaacgactg cgctcatatg
      421 cttcacttcc atcatccatg caagcatcat acaaacaaca ccgatgacgg aaccgatcga
      481 aatatcgatt cctcctgtga tcagtacaaa tgtcatacca acggctgcaa tgatcaatcc
      541 agcattacta atgaacaagt ttaaaaacac ctgtgggctt gtaaagtttc gatctccgta
      601 aaggatcgca cctgcaatat agagcacgaa gaataatacg atcgtaactg caagcaaaaa
      661 tccatttcct ctaagtttat ttttaatttt ctctaccatt gccattatgc caccttctct
      721 ccagcagttt ttgctgcacg ttttgcttta atacgattta agtatttctt aaattctgga
      781 gactgtaatg tcacgattat gataaccacg atcgctttat aaagtggaag ctgatctcca
      841 gttactttca ttgcatataa tgttgtagat aatgcctgga tcgtgatcgc tccaataaca
      901 cttcctgcga ctgagaattt accacctgca agagagtttc ctccaagtgc tactgctaag
      961 atcgcatcta attccatcaa gtttcctacg ttatttgcat caatggatgt gattcttgat
     1021 gttgcgatca ttcccgcgat tcctgcacaa agtccgcagg ctgcatatgt gataaagatg
     1081 atctttgtag aattcagtcc aaacaaacga ctggctcttg ggttgatacc tacactttca
     1141 atatacaatt ttgttgctgt cttatttaag aataatgttg ctaaaatgac tacgatgatc
     1201 gctaccagaa tcggtgttgg aactggaata tgtggcagaa atcctcctaa atacttaaag
     1261 ctgtccactt tgacatataa gttatttcca cctgtgatca actgtgccat tcctcgtcct
     1321 gcggtaaata agatcaaggt tgctaccatt ggctggatct tcatattcgc aactaagaat
     1381 ccgttccaca taccacataa tacagatacc acaagcccga tcaggattgc tgcgatccaa
     1441 ggaat
//
LOCUS       DAOMUE010000387         1426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_24776_length_1426_cov_1.628738, whole genome shotgun sequence.
ACCESSION   DAOMUE010000387 DAOMUE010000000
VERSION     DAOMUE010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24776_length_1426_cov_1.628738"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PLC71_11320"
     CDS             <1..624
                     /locus_tag="PLC71_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HRM03266.1"
                     /translation="RPDPELVESYRGIPSANIGDCVRKMTCMFDGIRSFNKKPLVGTA
                     FTVKVPSGDNLVAQMALDYAKPGDIIVVDGAGYTDRALVGGMMLAYAQERNLGGFVVN
                     GAVRDLDDITPLGPYRDGPGEVNVPVVCGGQVVMPGDILVGDIDGIVVVPRKDAAKLL
                     NDVHKNLEQEQREIKQMLEGTYTEEEHQEIFTGQFLKRGGSLEKTSY"
     gene            843..>1426
                     /locus_tag="PLC71_11325"
     CDS             843..>1426
                     /locus_tag="PLC71_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HRM03267.1"
                     /translation="MSKLQEPFRYDYVGSFLRTEELKKATTQYNENKITKEVYDNIVN
                     EEIAKLVAKQKELGYHVITDGEFRRTYWHLDFMWGFEGVAHEQTGSGVQFDGELASLE
                     DTYLVGKIKAKKHPFVEYFRYLKQFEDENTIAKYTIPAPAQTFQQMIVPANIETTRKY
                     YATNEELIQDIAVAYQDVIKQFYKAGCRNLQLDD"
BASE COUNT          531 a          197 c          310 g          388 t
ORIGIN      
        1 agaccagatc cagaacttgt agaaagttat cgtggaattc caagtgcaaa tatcggagac
       61 tgtgtccgaa aaatgacttg catgtttgat ggaatccgtt cctttaataa gaaaccatta
      121 gtaggaactg cgtttaccgt aaaagtacca tctggagata atcttgtcgc acagatggca
      181 ttagattatg caaaaccagg agacattatc gttgtcgatg gcgcaggata tacagacaga
      241 gctttagttg gtggaatgat gttagcatat gcacaggaaa gaaacttagg tggatttgta
      301 gtaaatggag ccgtcagaga tttagatgac atcacaccac ttggaccata cagagatggt
      361 ccaggagaag tcaatgtacc agttgtctgc ggaggacagg ttgttatgcc tggagatatt
      421 ttagttggag atatcgatgg aattgttgtt gtcccaagaa aagatgcagc aaagctgtta
      481 aacgatgttc ataaaaatct tgaacaggaa caacgagaga ttaaacagat gttagaagga
      541 acttacacag aagaagaaca tcaggaaatt tttacaggac agttcttaaa acgaggtgga
      601 agtttagaaa agactagtta ttaattaggt aactcttttt aaagtttatg ttttatatgt
      661 tggaaaagat cccatggaga tatgatttca tgggtttttt tctatcataa tgtctgtgaa
      721 ctgctttact ataacttgaa actatgacaa accgttaatt atatatattt taaaaaatga
      781 ttttgtgatg atatactctg tgtatagctt aaaaacaagg caaacaaagg agatacagca
      841 agatgagtaa attacaagag ccatttcgat atgattatgt aggaagcttt ttaagaacag
      901 aagaattaaa gaaagcaaca acacaatata acgaaaacaa gatcacaaaa gaagtatatg
      961 ataatatagt aaatgaagag atcgcaaaat tagtagcaaa acaaaaagaa ttaggatatc
     1021 atgtgatcac agatggagaa ttcagaagaa cttattggca tttggatttt atgtggggat
     1081 ttgaaggcgt agcacatgaa cagacaggaa gtggagtaca gttcgatgga gaattagctt
     1141 ctttagaaga cacttatctg gtaggaaaga tcaaagcgaa aaagcatcca tttgtagaat
     1201 atttcagata tttaaaacag tttgaagatg aaaatacaat tgcaaaatat acaatcccag
     1261 caccagcaca gacgtttcag caaatgatcg ttccggcaaa cattgaaaca acaagaaaat
     1321 attatgcgac aaatgaagag ttgatccagg atattgcggt tgcatatcag gatgtgatca
     1381 aacagttcta taaggcagga tgtcgtaatc ttcaattaga tgattg
//
LOCUS       DAOMUE010000388         1423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_24845_length_1423_cov_1.599415, whole genome shotgun sequence.
ACCESSION   DAOMUE010000388 DAOMUE010000000
VERSION     DAOMUE010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24845_length_1423_cov_1.599415"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..465
                     /locus_tag="PLC71_11330"
     CDS             148..465
                     /locus_tag="PLC71_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03268.1"
                     /translation="MKKLIMMAATLIMCFAFSLSVMAAPSPEDEYYNTSTEQEKVDEK
                     TQIDDDEFYKDEKPAKTNTTTTSTSASPKTSDQGAPVTLVYVSLAAAALAVYAAKHVK
                     EQY"
     gene            552..1280
                     /locus_tag="PLC71_11335"
     CDS             552..1280
                     /locus_tag="PLC71_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03269.1"
                     /translation="MIDIHTHILPGLDDGARDMEDSLSMAALALKSGVHTLVATSHAN
                     TGGFFKEYSIEEYLQRLEAFRKALDEVKLPLRVFSGMEIYMTREVPRMIREGILLSLN
                     QTGYFLTEFSFGIEQEEMDEMLHDLMELGVTPVIAHPERYRCVQESESGIAKWKNRGC
                     LLQVNRGSIFGRFGSGAKQCVDRLLDQHMVTCIASDAHKPYERTTYMADVKEYMEQEY
                     SFEYSKKLLYDNPKRILEGEKVVQ"
BASE COUNT          495 a          210 c          341 g          377 t
ORIGIN      
        1 tgataatatt cagagtatcg aatgtaacat caaagaaggt gagacaggat atgcagaata
       61 ttatccggat gagacaaaat tatttgagat gatattgaag gtattttata cacaaatcag
      121 ttagaatcca gtaagggggg atttagcatg aaaaagttga ttatgatggc agcaacattg
      181 attatgtgtt ttgctttttc actaagtgtt atggcagcac caagtcctga agatgagtat
      241 tataatacga gtacagaaca ggagaaggtt gatgagaaaa cccagattga tgatgatgaa
      301 ttctacaagg atgaaaaacc agctaagacg aacacaacaa cgacatcaac atctgcatca
      361 ccaaagacta gtgatcaggg agcgccagtt acattagtat atgtatcact cgctgcagca
      421 gcattagctg tatatgcagc aaaacacgtc aaagaacagt attaatcaaa aaacaaggtg
      481 gattcctcca gtcttgatgg actggaggaa ttctttttgc atatgggatt tctgaaagga
      541 aaataaaata gatgatcgat atacatacgc atattttgcc aggattggat gatggagcga
      601 gagatatgga agattctcta tctatggcag cattggcatt gaaaagtggt gttcatacat
      661 tagttgcaac atcgcatgcc aatacagggg gatttttcaa agaatattcg atcgaagaat
      721 atctgcaaag attagaagcg tttagaaaag cactggatga agtaaagctg ccattaagag
      781 tattctctgg aatggaaatt tatatgacaa gagaagttcc tcggatgatc cgtgaaggca
      841 tattgctttc attgaatcag acgggatatt ttttgactga attttcgttt ggaatcgagc
      901 aggaagagat ggatgagatg cttcatgatc tgatggaact tggagttacg ccagtgatcg
      961 cacacccaga gagataccgg tgtgtacagg aatcagaaag cggtatcgca aagtggaaaa
     1021 acaggggatg tctgttgcag gtaaacagag ggagcatctt tggcagattt ggatcaggag
     1081 caaaacaatg tgtagatcgt ctgctggatc aacatatggt aacttgtatc gcaagtgatg
     1141 cacacaaacc atatgagcgg acaacctata tggcagatgt caaagaatat atggaacaag
     1201 aatattcttt tgaatattca aagaaattgt tatatgataa tcctaagaga attcttgaag
     1261 gagaaaaggt ggtacaatga agaaattaaa attggtaagt tttgtgatat tttgtatatc
     1321 actggcagca agtctgatct tctggtttac acaggtcaga aaaggagacc ggatcggacc
     1381 ggtcattaca atggatcagc aggaattgac agtagggctc aat
//
LOCUS       DAOMUE010000389         1408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_25150_length_1408_cov_1.781227, whole genome shotgun sequence.
ACCESSION   DAOMUE010000389 DAOMUE010000000
VERSION     DAOMUE010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25150_length_1408_cov_1.781227"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /gene="rpsU"
                     /locus_tag="PLC71_11340"
     CDS             <1..117
                     /gene="rpsU"
                     /locus_tag="PLC71_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRM03270.1"
                     /translation="GNCAKAGIQQEIRKREHYEKPSVRRKKKSEAARKRKSK"
     gene            219..485
                     /locus_tag="PLC71_11345"
     CDS             219..485
                     /locus_tag="PLC71_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HRM03271.1"
                     /translation="MKKKPKEVIAKGLSLPRDVVFGDFIITLTGNCEAYIENYRGIVS
                     YDENKISIQGNHVILNICGCDLAVDFYSQESMKIKGKIKRLEYV"
     gene            510..>1408
                     /locus_tag="PLC71_11350"
     CDS             510..>1408
                     /locus_tag="PLC71_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HRM03272.1"
                     /translation="MLKILHFIFGRLYFQVKGYQTERFLNLCAKNHLVLWELKPVSDG
                     YVFFIRRSSYQMLEALAEKANVDLALQGQKGLPYFLKEHKKRKILFFSMCLCGLMILS
                     LSQFLWEITVSGGEVYSESDILKYVTDNYYHIGTLKYQIDCDALEDHLREDYAEIAWA
                     SCSIQGTRLHIEIKETLDRKTKQNPKKPCDIVANKSGIITKMAVKSGTPLVSVGDSVK
                     SGTTLISGLIYYYSDDFTVTQTDKIKADGEIVMRTKEVYNNSIPMEYYEKLITGKKKS
                     IKNLYFGQYQFDFPEKKRQDHTNV"
BASE COUNT          510 a          198 c          309 g          391 t
ORIGIN      
        1 ggaaactgtg caaaagcagg aattcagcag gagattcgta aaagagaaca ttacgaaaag
       61 ccaagcgtaa gacgtaagaa aaagtctgaa gcggctcgta agagaaaatc taaataattt
      121 ttacttgatt gaaaagataa gcatcattta cctgggaaca ggtatatgat gctttttttc
      181 ggcatatatt aaaccaaatg ccacaaaagg agttttgcat gaagaagaaa ccaaaagaag
      241 tgattgctaa aggactttct cttccaagag atgtggtgtt tggagatttc attataacct
      301 tgacaggaaa ctgtgaagca tacatagaaa attacagggg gatcgtatca tatgatgaga
      361 ataagatctc cattcagggg aatcatgtta tattaaatat ctgtggatgt gatctggcag
      421 ttgattttta ctcacaggaa tcaatgaaga tcaaaggaaa gatcaaaagg ctggaatatg
      481 tatgatattc tttgaaagga gcatgagaaa tgcttaaaat tctgcacttt atatttggaa
      541 ggctgtattt tcaagtcaaa ggatatcaga cagagcgttt tttgaatctc tgtgcaaaaa
      601 atcatcttgt attgtgggaa ttaaaaccag tatcagacgg ctatgtgttt tttatcagac
      661 gaagttctta tcaaatgttg gaagcattag cagagaaagc aaatgttgat cttgcgttac
      721 aagggcaaaa aggactgcca tatttcttaa aagaacataa aaaacgtaaa atattatttt
      781 tttccatgtg tttgtgcggt ttaatgatcc tttcgctgag tcagttttta tgggagatta
      841 cagtttctgg aggagaagta tattcagaaa gtgatatatt aaagtatgtt acggataact
      901 actaccatat tggaacatta aaataccaga ttgactgtga tgcattggaa gaccatctga
      961 gggaagatta tgctgagatt gcgtgggcat cctgttcgat tcagggaaca agactgcata
     1021 ttgagataaa agaaacttta gatcgtaaaa caaagcagaa tccaaagaaa ccatgtgata
     1081 tcgtggcaaa taaatcaggg atcattacaa aaatggcggt caaaagtgga acaccgcttg
     1141 taagtgttgg ggacagtgtc aaaagcggaa caactttaat ttctggattg atctattact
     1201 acagtgatga ttttactgtg acacagacgg ataagatcaa agcggacgga gagatcgtga
     1261 tgcgcactaa agaggtgtac aacaattcaa ttcccatgga atattacgag aagctgatca
     1321 caggcaagaa aaagagtatc aaaaatttat attttggaca gtatcaattc gattttcccg
     1381 aaaagaaaag acaggatcat acaaatgt
//
LOCUS       DAOMUE010000390         1408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_25155_length_1408_cov_1.501109, whole genome shotgun sequence.
ACCESSION   DAOMUE010000390 DAOMUE010000000
VERSION     DAOMUE010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25155_length_1408_cov_1.501109"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PLC71_11355"
     CDS             complement(<1..860)
                     /locus_tag="PLC71_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HRM03273.1"
                     /translation="MSKTYYVCKYTPIELLQAFGGECENLNQMPEGFDLADQIAHPNI
                     CGFGKAVLEAVMTGKVKELVLVNCCDTIRSVYDILEDSGKLDFLYMIDMLHCDGECSR
                     ERTVLQLKGLARAYGAYKGSEFDQKKFVEAFKEPEKQKEPYISVLGARMGNELYEMVK
                     ESMPYSVENDTCVNNRSVGEVKPPKTDDFNELMEWYAAELLGQTPCMRMMDNSGRKRL
                     YNDPNLKGIIYHTVKFCDFYSFEYAQVKQNITVPLLKIESDYTVQSSGQLLTRLEAFA
                     ESMNMDNLESG"
     gene            complement(853..>1408)
                     /locus_tag="PLC71_11360"
     CDS             complement(853..>1408)
                     /locus_tag="PLC71_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HRM03274.1"
                     /translation="AGMTNNILLGTKEEEKYVDQLLKDIEKAPKKKGKNIYWMHTIPF
                     WSGAVQEALAFNENAQIAGCELSRTFEPDFDPEDPYDAMARRMVYHGLNGSAVRRIEA
                     GIRHAKDVKADGAVWFGHWGCKHTLGAAQLAKKKFEEAGIPMLLLDGDGCDRSHGGEG
                     QTSTRLGAFLEMLGAEEMEGGQDE"
BASE COUNT          343 a          352 c          207 g          506 t
ORIGIN      
        1 ccactttcta aattgtccat attcatgctt tctgcaaatg cttctaatct tgtcagtaac
       61 tgtccgctgc tctgcactgt atagtctgac tcgatcttta ataatggcac tgtaatgttt
      121 tgttttacct gtgcatactc aaaactgtag aaatcacaaa atttgaccgt atggtaaatg
      181 atccctttta agtttggatc attatacaaa cgttttcttc cgctgttatc catcatacgc
      241 atacaaggtg tctgtcctaa aagttctgct gcataccatt ccattaattc attaaaatca
      301 tctgtctttg gtggctttac ttcaccgacg cttcggttat tgacacaagt atcattctcc
      361 actgaatatg gcatggattc tttgaccatt tcatataatt catttcccat tcttgcccca
      421 agaacactga tatatggttc tttctgcttt tctggttctt taaatgcttc aacaaatttc
      481 ttttgatcaa attcagatcc tttgtaggct ccataggctc ttgcaagtcc ttttaactgt
      541 aaaacagttc gttccctgct gcattctccg tcacagtgca gcatatcgat catatataaa
      601 aaatcaagtt ttccactgtc ttccagaata tcatagacac ttcggatcgt atcacagcag
      661 tttactaaaa ccagttcttt tactttccca gtcataactg cttctaagac agcttttcca
      721 aatccacaga tatttggatg tgcgatctga tctgcgagat caaatccttc cggcatctgg
      781 tttaaattct cacattcccc gccaaatgcc tgcaataatt caattggtgt atatttacaa
      841 acgtaatatg tcttactcat cttgtccacc ttccatttct tcagctccaa gcatctctaa
      901 aaatgctcct agtcttgttg aagtctgtcc ttctccgccg tggcttcggt cacatccatc
      961 tccgtctaat aacagcattg gaattcctgc ttcttcaaac ttcttcttgg caagctgtgc
     1021 tgctccaagt gtatgtttac atccccagtg tccaaaccag acagctccat ctgctttcac
     1081 atcttttgca tggcggattc cggcttcaat tcttcttacc gcacttccat ttaatccatg
     1141 atagaccatt cttctcgcca tcgcatcgta tggatcttct ggatcaaaat caggttcgaa
     1201 tgtacgactt aactcgcatc ctgcgatctg tgcattctca ttaaatgcca gtgcttcctg
     1261 tactgcacct gaccagaatg ggatcgtgtg catccagtag atattctttc cttttttctt
     1321 tggtgctttt tcgatatctt ttaataactg atccacatat ttctcttctt cttttgttcc
     1381 caacaaaata ttatttgtca ttcctgca
//
LOCUS       DAOMUE010000391         1391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_25501_length_1391_cov_2.098054, whole genome shotgun sequence.
ACCESSION   DAOMUE010000391 DAOMUE010000000
VERSION     DAOMUE010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25501_length_1391_cov_2.098054"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="PLC71_11365"
     CDS             complement(<1..783)
                     /locus_tag="PLC71_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRM03275.1"
                     /translation="MTGTFWAILPPVIAIVLALITKEVYLSLMIGIASGALMYHNFHL
                     IKAMDTIFAIMGEKIGANCDILIFLVLLGILVALITKSGASRAYGKWAAKAIRTKRQA
                     LFATICLGVVIFVDDYFNCLTVGTVMRPVTDKHNVTRAKLAYVIDATAAPVCIIAPIS
                     SWAAAVGSSLPAGCNIDGFTLFLRAIPFNYYALLTLVFLAFLIITNIDYSKMKQYELN
                     AHLEEIVEDEEEVPIVGHGKVKDLILPILFLIASCIGFMLYTG"
     gene            complement(804..>1391)
                     /locus_tag="PLC71_11370"
     CDS             complement(804..>1391)
                     /locus_tag="PLC71_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HRM03276.1"
                     /translation="SGETSSYRSNPLGLAEFALSRKDPAYVGLAKEVQKAQKAIEAGE
                     DAAEAFPEVKDILETVKESYADVTQDNLGIGSTIFAVKPGDGSAREQAASCQKVLGGW
                     ANIANEYATKRYRSNLINWGMLPFTIDKGELPFKNKDYIFVPDVRKAVEDKLTKIPAY
                     VVNEGMKEITLTLGELTDDEREIILKGCLINYYRD"
BASE COUNT          399 a          313 c          229 g          450 t
ORIGIN      
        1 tcctgtgtac aacataaatc caatacaaga agcgatcaaa aataaaatcg gcaagatcag
       61 atcttttacc tttccatgtc caacgatcgg tacttcttct tcatcttcaa cgatctcttc
      121 taaatgtgca tttaattcat attgtttcat ctttgaataa tcaatattcg taatgatcaa
      181 aaatgccaaa aatacaagtg tgagcaatgc ataatagtta aatggaatcg ctctcaaaaa
      241 taaggtaaat ccatcaatat tgcatcctgc tggaagagat gatcctacag ctgctgccca
      301 gcttgagatt ggcgcaataa tacatacggg agctgcagtt gcatcgatca cataagccaa
      361 ttttgctctt gtaacattat gtttatctgt cacagggcgc ataaccgttc caactgtcag
      421 acagttaaaa taatcatcaa caaagatcac aactccaaga cagatcgttg caaaaagtgc
      481 ctgtctcttt gtgcggatcg cttttgctgc ccattttcca taggctctag aagcacctga
      541 tttggtgatc agtgctacca gaattcctaa taatactaaa aatatcaaaa tatcgcaatt
      601 agctccaatt ttctctccca tgatcgcaaa aatggtatcc attgctttga tcagatgaaa
      661 attatgatac atcagggccc ccgatgcaat tccgatcatc agcgataaat atacctcctt
      721 ggttattaat gctaaaacaa ttgcaatcac tggcggaaga atcgcccaaa atgtacctgt
      781 catgtatatt tactccttaa tctttaatct ctgtaataat tgatgaggca tccttttaaa
      841 atgatttctc tctcatcatc tgttaattca cctagtgtta aggtgatctc tttcattcct
      901 tcatttacca catatgctgg aatctttgta agcttgtctt ccactgcttt gcggacatct
      961 ggaacaaaaa tgtaatcttt gttcttaaat ggaagttctc ctttatcgat cgtaaatgga
     1021 agcattcccc agttgatcag gtttgaacgg tatctctttg tcgcatattc atttgcgata
     1081 tttgcccatc cacctaatac tttctggcaa gaagccgcct gttcacgtgc agaaccatct
     1141 cctggtttca ctgcaaagat cgtgcttccg attcctaaat tatcctgagt tacatccgcg
     1201 taagattctt tgactgtctc caggatatct ttaacttctg ggaatgcttc cgctgcatct
     1261 tctccagctt cgatcgcttt ctgtgctttc tgtacttctt ttgcaagtcc tacatatgca
     1321 gggtcttttc ttgataacgc aaattctgca agtcctaatg ggttagaacg ataagatgaa
     1381 gtctctcctg a
//
LOCUS       DAOMUE010000392         1366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_26131_length_1366_cov_2.276888, whole genome shotgun sequence.
ACCESSION   DAOMUE010000392 DAOMUE010000000
VERSION     DAOMUE010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26131_length_1366_cov_2.276888"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLC71_11375"
     CDS             complement(<1..621)
                     /locus_tag="PLC71_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRM03277.1"
                     /translation="MEITKQQIIHTDVLIIGGGTAGCYAALTIREKSDAKIVIAEKAN
                     IKRSGCLAAGVNAINAYIVKGRKPQDYVDYARKDADEIVRGDLLLTMSEHLNEVTSKM
                     EQLGLVILKDENGDYVARGNRNIKINGENIKPILADAVNQLDNVEVINHLNITDYIVE
                     DNTIKGAFGFHMENGTAYEICAKKVLCATGGAAGLYKPNNPGFSRHK"
     gene            complement(646..>1366)
                     /locus_tag="PLC71_11380"
     CDS             complement(646..>1366)
                     /locus_tag="PLC71_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM03278.1"
                     /translation="ELLELTGLSGLENRYPNQLSGGQRQRVAFARALAPNPQVLLLDE
                     PFAAIDAKVRSELRLWLKEMVSKLGITSIFVTHDQDEAVEVADEILITNHGKIEQMGS
                     PLEIYKSPKTPFVAQFVGRSSIVENYESLKGFEKIEHAQKAIVRPEFLELAKKGELKR
                     YMSASEKGIVEDVIFSGSRLDVVVNINGIKVTAERSLEKDPVSVGEEVDIIIYRLYVF
                     DEKETYLLENKEMQEGDVFYI"
BASE COUNT          342 a          325 c          206 g          493 t
ORIGIN      
        1 cttatgtctt gaaaatccag gattatttgg cttataaagc cctgctgccc ctcctgttgc
       61 acaaagtact ttcttggcac agatctcata agctgttcca ttttccatat ggaaaccgaa
      121 ggctcctttg atcgtgttat cttccacgat ataatcggtg atattcaaat gattgatcac
      181 ctctacgtta tctagctgat ttactgcatc tgcaaggatt ggcttgatat tttctccatt
      241 gatcttaata tttcgatttc ctctggcaac atagtcacca ttttcatcct ttaaaataac
      301 caatcctaac tgttccatct tgcttgtcac ttcatttaaa tgttctgaca ttgtaagaag
      361 caggtcacct cgtacgatct cgtctgcatc ttttctcgca taatcaacat aatcctgtgg
      421 ttttcttccc tttacgatat aagcattgat cgcattaact ccagctgcta agcacccgct
      481 tcgtttgata ttggctttct cggcgatgac gatctttgca tcactttttt ccctgatcgt
      541 aagtgccgca taacatccag ctgttccacc tccgatgatc aggacatcgg tatgtatgat
      601 ctgttgtttt gtaatttcca taatcggtcc tttctggtat atttcttaaa tatagaagac
      661 atcgccttcc tgcatttctt tattctctaa taaataagtt tccttttcat caaatacata
      721 taaacggtaa ataatgatat caacttcttc tccaacgctt accggatcct tttccaaaga
      781 tctctcagct gtcactttga ttccattgat atttacaact acatccagtc gacttccact
      841 aaagatcaca tcttctacaa ttcctttttc tgatgcactc atatatcgtt ttaattcccc
      901 tttttttgct aactcaagga attctggtct gacgatcgct ttctgtgcat gttctatttt
      961 ttcaaatcct tttaaggatt cataattttc tacgatggat gatcttccaa caaactgggc
     1021 tacaaatggc gtctttggtg atttataaat ctctaatggg cttcccatct gctcaatctt
     1081 tccatgattt gtaatcaaga tctcatcagc aacctctacg gcttcatcct gatcatgtgt
     1141 aacgaaaatg cttgtgatac caagctttga taccatttct tttagccaga gtcgaagctc
     1201 tgatcttact tttgcatcga tcgctgcaaa tggttcatca agaagtaata cctgtggatt
     1261 tggagccaat gctcttgcaa atgcaactct ctgtctctgc ccgccagata actggttcgg
     1321 atatctattt tcaagtcctg ataaccccgt taattccagt aattca
//
LOCUS       DAOMUE010000393         1357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_26361_length_1357_cov_1.865591, whole genome shotgun sequence.
ACCESSION   DAOMUE010000393 DAOMUE010000000
VERSION     DAOMUE010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26361_length_1357_cov_1.865591"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..>604
                     /locus_tag="PLC71_11385"
     CDS             58..>604
                     /locus_tag="PLC71_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM03279.1"
                     /translation="MIAIYLAPLYLLLNVYFLFRILKWLETCHVYFKKKWIKAGLVMI
                     YVFLAFSILIAFLFPQGTIRRAMKLISNYWLGVLMYLALTILIADLIRLILIYFVKAD
                     QKKFRTPKVFRIVGSICMILILLISFYGVCNARNIRTTSYHVTIHKKAGNHKKLKIIL
                     LADLHLGYNIGCSQMKQMVTKV"
     assembly_gap    605..614
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <615..1253
                     /locus_tag="PLC71_11390"
     CDS             <615..1253
                     /locus_tag="PLC71_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRM03280.1"
                     /translation="SPDLIVVAGDIFDNEYDALDDPDQLVKIFRQLKSQYGVYAVYGN
                     HDIDEKILAGFTFGSGREKKVSDPRMDEFVKRAGMKLLRDESVCIDQSFYLYGRPDAE
                     KVGRGISRRKTPKELVNGMDLKKPVIVLDHEPRQLEELNQAGVDIDLCGHTHDGQLFP
                     GNITIHLFWKNPYGYRKVGNAHQIVTSGVGLFGPNMRVGTKAEIVVVNVDFR"
BASE COUNT          449 a          183 c          314 g          401 t
ORIGIN      
        1 aatgaatttg ataaaaaatc aataaaatat agtaagaaag agggtgcaga aaataagatg
       61 atcgcaatat atttagcacc actttatctg ctgttaaatg tctatttttt attccgaata
      121 ttgaaatggt tagagacttg tcatgtttat tttaaaaaga aatggatcaa agcaggtttg
      181 gtcatgatct atgtatttct tgcattcagt attctgatcg catttttgtt tccacaagga
      241 acgatacggc gagctatgaa actgatcagt aattactggc tgggtgtttt aatgtatttg
      301 gcgcttacga tccttattgc ggatctgatc cgcctaatct taatctattt tgtaaaagca
      361 gatcagaaaa agtttcggac accaaaagtt ttcaggattg ttggaagcat ttgtatgatc
      421 ttgatcttat tgatcagttt ttacggagtg tgtaatgcaa ggaatatcag gacaacaagc
      481 taccacgtaa cgatacataa aaaagcagga aatcataaaa agctaaagat cattttactt
      541 gcagatcttc atcttggata caatattgga tgttctcaga tgaaacagat ggtaacaaaa
      601 gtaannnnnn nnnnagtcca gatctgatcg tggtcgcagg agatattttt gataatgaat
      661 atgatgcatt agatgatcca gatcagttgg tcaagatatt tcgacagcta aagagtcaat
      721 acggtgttta tgcagtttat ggaaatcatg atattgatga aaagattctt gcaggattta
      781 catttggcag cggtcgggag aaaaaggtca gcgatccaag aatggatgaa tttgtaaaaa
      841 gggcaggaat gaagttatta agagatgaat ctgtttgtat tgatcaaagc ttctatctct
      901 atggaagacc agatgctgag aaagtaggcc gtgggatcag cagacgtaag actccaaaag
      961 aactggtaaa tggaatggat cttaaaaagc ctgtgatcgt actggatcat gagccaaggc
     1021 agttggaaga gttaaatcag gcaggagtag acattgatct ttgcgggcat acgcatgatg
     1081 gacagctgtt ccctgggaat attacaattc atttgttttg gaagaatccg tatggatatc
     1141 gtaaggttgg aaatgcgcat cagattgtaa cttcaggtgt cggtttattt ggcccgaata
     1201 tgagggtggg aacgaaagcg gagattgtgg ttgtgaatgt ggattttaga tgatctgaca
     1261 aagatgtcag ttatactata ataaacataa tggaggaaga catttttatg aaatatcacg
     1321 atggaaaaga cagatgcagt tgggctaatc caaagaa
//
LOCUS       DAOMUE010000394         1334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_26884_length_1334_cov_1.954652, whole genome shotgun sequence.
ACCESSION   DAOMUE010000394 DAOMUE010000000
VERSION     DAOMUE010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26884_length_1334_cov_1.954652"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>526
                     /locus_tag="PLC71_11395"
     CDS             <1..>526
                     /locus_tag="PLC71_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HRM03281.1"
                     /translation="YENQLKIKEGMVRDLLKDYVNDDIWEEIKGSPKVHGYRNKMEFS
                     FGDEVKDGPLALGMHKKNTFHDIVDNDYNLIVKCALNIAQQMELPFYHKMRHEGYFRH
                     LVVRRAESSGDVLVNIVTTSQVEADLTKLRDALLELPLSGKIIGILHTTNDSLADVVQ
                     ADKIDILYGQDYFY"
     assembly_gap    527..536
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <537..1085
                     /locus_tag="PLC71_11400"
     CDS             <537..1085
                     /locus_tag="PLC71_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009249249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRM03282.1"
                     /translation="ILGLKFKISPFSFFQTNTLGAEILYKTARDFVGETKDKVIFDLY
                     SGTGTIAQMLAPVAKKVVGVEIVEEAVEAAKVNAELNGLDNCEFIAGDVLKVVDELED
                     KPDFIVLDPPRDGIHPKAIQKIIDFGVEQMVYISCKPTSLARDLEVFEAAGYKVKRAT
                     AVDQFPNTVHIESMVLIQKDNI"
BASE COUNT          472 a          175 c          303 g          374 t
ORIGIN      
        1 cttatgagaa tcagttaaag atcaaagaag gtatggtacg tgatctgtta aaagattatg
       61 taaatgatga tatatgggaa gaaattaaag gaagtcccaa agtgcatggg tatcgcaata
      121 aaatggaatt ctcttttggt gatgaggtaa aagacggtcc attagcattg ggaatgcaca
      181 aaaagaacac attccatgat attgtagaca atgattacaa tctgatcgta aaatgtgcat
      241 taaatatcgc acaacagatg gagctgccat tctatcataa gatgcgtcat gaaggatatt
      301 tcagacattt agttgtaaga agagcagaaa gttcgggaga tgttttggta aatattgtaa
      361 cgacatcgca ggtagaagca gatcttacca aacttcgaga tgcattatta gaattaccat
      421 tgagtggaaa gatcattggg attcttcata caacaaacga cagccttgca gatgtggtac
      481 aggcagacaa aatagatatt ttatatggac aggattattt ctatgannnn nnnnnnattt
      541 taggattaaa attcaagatt tcaccatttt cattcttcca gacaaacaca ttaggggcgg
      601 agatacttta taagactgcc agagattttg ttggggagac aaaagataaa gtgatctttg
      661 atctgtatag tggaactgga acgatcgcgc agatgttagc accagttgct aagaaagttg
      721 ttggagtcga gatcgttgag gaagcggtag aggcagcgaa agtcaacgca gaattaaatg
      781 gattggataa ttgtgaattt attgcaggag atgtattaaa ggttgtggat gaattagaag
      841 ataagccaga ctttatcgta cttgaccctc ctagagatgg aatccatcca aaagcgattc
      901 agaagattat tgatttcggg gttgaacaga tggtatatat cagttgcaaa ccgacaagtc
      961 ttgcgagaga tctggaagtg tttgaggcag caggatataa agttaagcga gctacagcgg
     1021 tagatcagtt cccgaatacg gttcatatcg agagtatggt gttgatacaa aaggacaata
     1081 tctaaaaatc cttgaattta agcggtttgt gaagcatttt agttttaaga aaataagtga
     1141 aacttacagt caaaaagagc ttttagaaga aattacagtt gttgtagctc ttgatgtcgt
     1201 tgagagggac acaagaaatc ctagttttat aaatgaaagt aaatcatcgg acagatgata
     1261 aaaaataggg atactttaat tacaaagtat ctctattttt tatacaaaat ttataaagaa
     1321 ggagaattga atac
//
LOCUS       DAOMUE010000395         1326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_27074_length_1326_cov_2.598741, whole genome shotgun sequence.
ACCESSION   DAOMUE010000395 DAOMUE010000000
VERSION     DAOMUE010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27074_length_1326_cov_2.598741"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..977)
                     /locus_tag="PLC71_11405"
     CDS             complement(18..977)
                     /locus_tag="PLC71_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HRM03283.1"
                     /translation="MKSDRFLIKAAELYYRDGLSQQEIAKKLHTSRTSISRALIQARN
                     EGYVQIRIQYPEQSDLALERKLEEKYGLTEALIAVPAYEQSSDQEVAFQAVDYILRVL
                     KKNMVFGMTWGRAMHEFVEQLAKDERLRSLSFQNVKVVPFLGTPGAIQSDSWNTTTYS
                     NTLATKVGNLLHCASYNLSAPMYVEGTKEKELIESIDEIAKVLHMAETADIALIGIGS
                     MQDDSSIIKAEIRTKEEYKELVRKGAVGEIVGRIYDKNGQTVDEDLKRKMVGISLDKI
                     AKIPVRVGMSYGKDKIEAIKGAIAGGLVNVLVTDVPTAELLLK"
BASE COUNT          352 a          282 c          189 g          503 t
ORIGIN      
        1 atattcttat ctctatatta ttttaataat aactctgccg tcggcacatc tgttaccaac
       61 acattcacta atccacctgc gatcgcacct ttgattgctt cgatcttatc ttttccataa
      121 gacattccga ctctgactgg tatctttgcg atcttatcca atgaaattcc aaccattttt
      181 cttttcagat cctcatcaac cgtctggcca ttcttgtcat aaatccttcc aacgatttct
      241 ccaacggcac cttttctcac tagttccttg tattcttcct ttgtccgtat ttctgccttg
      301 ataatcgaag aatcgtcctg catcgatccg attccgatca atgcaatgtc cgctgtctct
      361 gccatatgaa gaactttggc gatttcatca atactttcga tcaactcttt ttcttttgta
      421 ccttccacat acattggtgc agacaagtta taacttgcac agtgtaataa atttccgact
      481 ttcgttgcaa gtgtattgga atatgttgtt gtattccatg aatctgactg tatcgctccc
      541 ggtgttccaa gaaatggaac aacttttaca ttctgaaatg aaagagatct aagacgttca
      601 tctttcgcca actgctccac aaattcatgc atggctcttc cccatgtcat tccaaatacc
      661 atgttttttt tcaaaactct tagaatatag tctaccgctt gaaacgctac ttcttgatcc
      721 gatgactgtt catatgccgg aacagcgatc aatgcttctg tcaatccata tttttcttct
      781 aatttccgtt ccaatgcaag atcactttgt tcaggatatt gaatcctgat ctgaacatac
      841 ccttcatttc tcgcctggat caatgctctg gaaatactgg ttcttgatgt atgtaatttt
      901 ttcgcaatct cttgttgcga taatccatca cggtaataaa gttctgctgc ttttattaaa
      961 aaacgatcac ttttcatctg gcttcctccg tcgtgaatat gccacatttc atatgtgact
     1021 ttctatattt atattatctg gttttttatt tattttcaat gcacattttt atattttctt
     1081 aatctgtttt atcggatatt ttgattgtac gaaaacaaag atcatgatct tatcttttta
     1141 tcagatttta cgctttctta acgttttatt tactaaattt tataattttt gtatataatt
     1201 atatgttaaa atagtagtta actaatgtta gatggtttaa atacaaatat aaaaccagaa
     1261 gggagataaa ttcatgttaa gaattggaat tttaaccagt ggcggcgact gtcaggcatt
     1321 gaatgc
//
LOCUS       DAOMUE010000396         1314 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_27416_length_1314_cov_1.965052, whole genome shotgun sequence.
ACCESSION   DAOMUE010000396 DAOMUE010000000
VERSION     DAOMUE010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27416_length_1314_cov_1.965052"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..666
                     /locus_tag="PLC71_11410"
     CDS             10..666
                     /locus_tag="PLC71_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRM03284.1"
                     /translation="MKQILCFGDSNTYGLIPGTTRRYDRETRWTGILAEKLYDKGYRI
                     IEEGLCGRTSVFDDATRDGRNGAKVLPMLLETHAPLDQVVLMLGTNDCKTYNHASADR
                     IGKGIEKLIQQIRKADSAIDILLVSPIELGEDVWKPGFDLEFDQHSVKVSKQLKQTYL
                     KIAWKYGCDFLAASDVAKPSKADMEHMNEKGHKNLAKAIESFLVEKERNHSNNYVNVV
                     "
     gene            739..>1314
                     /locus_tag="PLC71_11415"
     CDS             739..>1314
                     /locus_tag="PLC71_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HRM03285.1"
                     /translation="METINPQEWKQDLPAFKEKTDQFYAGELSKADYKGFSGGYGSYA
                     QKDGKASMIRLRMSGGHLTKDKLKFIADSIKTYNVDKVHLTTCQTIQLHNLQPEAIYG
                     IMEGGLDHGIVTMGGGGDFPRNVTVSPLSGVEKGEYFNVLPYAEAAGEYLMTFIKKEV
                     MPRKLKVGFSNGPANETHATFRDLGFVAREDG"
BASE COUNT          500 a          193 c          290 g          331 t
ORIGIN      
        1 gtgacagata tgaaacagat attatgcttt ggagattcca atacatatgg actgatccca
       61 ggtacaacaa gaagatatga cagagaaaca agatggacag gaattttagc agagaagctt
      121 tacgataaag gatatcgaat tatagaagaa ggattatgtg gaagaaccag tgtatttgac
      181 gatgcgacaa gagatggaag aaatggagcg aaagtacttc caatgctctt agagacacac
      241 gcaccacttg accaggttgt tttaatgctt ggaaccaatg actgcaaaac atataaccat
      301 gcaagtgcag atcgcatcgg aaaaggaatc gaaaaactga tccagcagat cagaaaagct
      361 gattcagcga tcgatatttt attagtatct ccgatcgaat taggagaaga tgtatggaaa
      421 ccaggatttg atcttgaatt tgaccagcat tctgtgaaag tatccaagca attaaaacag
      481 acttatttaa agattgcatg gaagtatgga tgtgatttcc ttgcagcaag tgatgtagcc
      541 aagccaagta aggcagatat ggaacatatg aatgaaaaag gacataaaaa ccttgcaaaa
      601 gcaatcgaat catttcttgt agaaaaggaa agaaatcatt caaacaatta tgtaaatgtg
      661 gtatgataag acaaatgtgg tattttataa ggattcacat aataaaagaa ataaaatgtt
      721 cagaaaggaa ttttacatat ggaaacgatc aatccacagg aatggaaaca agacctgcca
      781 gcatttaaag aaaagacaga ccagttttat gcaggagaat taagtaaagc agattacaaa
      841 ggattttccg gtggttacgg aagttatgca cagaaagatg gaaaagcaag tatgatccgt
      901 cttcgtatgt caggtggcca tttaacaaaa gataaattaa aatttatcgc agacagtatt
      961 aaaacatata atgtagataa agttcacctg acgacatgtc agacgattca gttacataac
     1021 ttacagccag aagcaattta tggaatcatg gaaggtggat tagatcatgg tattgtcaca
     1081 atgggtggag gtggagattt tccacgtaat gtaaccgtat ctccattgtc aggtgttgaa
     1141 aaaggagaat attttaacgt tcttccatat gccgaagcag ctggagaata tttgatgaca
     1201 tttatcaaaa aagaagttat gccaagaaaa ttaaaagttg gattttcaaa cgggccagct
     1261 aatgagacac atgcaacatt ccgtgacctt gggtttgtgg caagagaaga tgga
//
LOCUS       DAOMUE010000397         1299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_27791_length_1299_cov_1.367363, whole genome shotgun sequence.
ACCESSION   DAOMUE010000397 DAOMUE010000000
VERSION     DAOMUE010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27791_length_1299_cov_1.367363"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..>497)
                     /locus_tag="PLC71_11420"
     CDS             complement(9..>497)
                     /locus_tag="PLC71_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRM03286.1"
                     /translation="IKVVREVGKEYPEIEIQERLVDAMCAKMLDPEFNKGIEVVVLPN
                     LYGDIVTDVAAEHQGGLGTASSSNIGNKYALFEAIHGTAPYLMSHGRGHYADPSSLIR
                     AMGQMLQHIGFGDRNDILEKAMDICTIEERKVVLTTFTEDASTKEFADYLLETIKKIK
                     EA"
     assembly_gap    498..507
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<508..718)
                     /locus_tag="PLC71_11425"
     CDS             complement(<508..718)
                     /locus_tag="PLC71_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HRM03287.1"
                     /translation="MDWTFFRENIEGEYILGNRGIQVNDDLAIDFKVQTKQGTERIAR
                     AAFEFARKNGKKNVTIVTKANIVKLV"
     gene            complement(775..1125)
                     /locus_tag="PLC71_11430"
     CDS             complement(775..1125)
                     /locus_tag="PLC71_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03288.1"
                     /translation="MENIKRAQEEFKELIESEYARIDKMKQAEEATDFSKKEKIIVGI
                     LPGDGIGPIIMEQAVRVVKELVKDEIEAGKIELREIEGMTIENRVAKMESLPSDVYQE
                     LVMDFQTLSVQTVY"
BASE COUNT          322 a          278 c          202 g          487 t
ORIGIN      
        1 cttttaattt atgcttcttt gatctttttg attgtctcta acagataatc agcgaattct
       61 tttgtacttg catcttctgt aaatgttgtt aatacaactt ttctttcttc gatcgtgcag
      121 atatccattg ctttttcaag gatgtcattg cggtctccaa atccgatgtg ctgcaacatc
      181 tgtcccattg cacggatcaa gctgcttgga tctgcataat gtcctcttcc gtggctcatc
      241 agatatggtg ctgttccatg gattgcttca aataatgcat atttgttacc aatgttagaa
      301 gacgatgctg ttcctaaacc accctgatgt tctgctgcaa cgtctgttac gatatctccg
      361 taaaggtttg gaagaacaac aacttcgatt cctttgttaa attcaggatc taacattttt
      421 gcacacattg catcaactaa gcgttcctga atctcaatct ctggatattc ttttccaact
      481 tcacgaacaa ctttgatnnn nnnnnnncaa ccagttttac gatgtttgct tttgtaacga
      541 tcgttacatt tttctttccg ttctttcttg caaattcaaa tgctgctctt gcaattcttt
      601 ctgttccctg ttttgtctga actttaaagt cgatcgcaag atcatcattt acctgaattc
      661 cgcggtttcc aaggatgtat tctccttcaa tattctcacg gaagaatgtc cagtcaattc
      721 ctttgtcttt gatcttgatc ggacgaactg ctgcaaataa ttcaagtcct cttcttaata
      781 aactgtttgc actgacaagg tttggaagtc catcaccagc tcttggtata catcagatgg
      841 aagactttcc atttttgcaa cacgattttc aattgtcatt ccttcgattt ctcgaagttc
      901 gatctttcct gcttcaatct catctttgac aagttctttt acaacacgaa ctgcctgctc
      961 catgatgatc ggcccgattc catctccagg aaggatacca acaatgatct tttctttttt
     1021 gctaaaatct gttgcttcct ccgcctgttt catcttatcg attcgtgcgt attcactttc
     1081 aataagttct ttgaactctt cctgtgctct tttaatattc tccataattc ctcctgttaa
     1141 aactccgttt ccgttggctt gtttttcttt atggttttat tataatgcag aaattattga
     1201 aaaataaaat tgacaaaaag tatgatggta ttccatttcg ttatatgtaa tttgaaggta
     1261 gtcgttcaaa tgtatatgag aaaaacgaaa taataatag
//
LOCUS       DAOMUE010000398         1293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_27933_length_1293_cov_2.768174, whole genome shotgun sequence.
ACCESSION   DAOMUE010000398 DAOMUE010000000
VERSION     DAOMUE010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27933_length_1293_cov_2.768174"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PLC71_11435"
     CDS             complement(<1..535)
                     /locus_tag="PLC71_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRM03289.1"
                     /translation="MDKEHRVSVYDMVKELNLKEVTPEINSKEVYIEQAEINRPALQL
                     AGFFENFAQNRVQIIGKAEHLFIEEVEKDVENAKNIYKRFVGAGIPVIVFCRNLHPSE
                     MMIKVAKEYGVPVLISQDTTSEFMAEVIRWLGTVLAPSITIHGVLVDVFGEGILITGE
                     SGIGKSEAALELIRRGHR"
     gene            complement(597..>1293)
                     /locus_tag="PLC71_11440"
     CDS             complement(597..>1293)
                     /locus_tag="PLC71_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRM03290.1"
                     /translation="EGLNRMEAFDISNISGFQTVASMVVFERGKARRRDYRKFRLRTI
                     EGPDDYASMEEVLTRRFLHGKKELEELKEQGKDTTLGSFTKFPDILMMDGGKGQVNVA
                     LRVLDHLGLDIPVCGMVKDDDHRTRGLYYNNEEIIFAPNSEAFLLVTRIQDEAHRFAI
                     EYHRSLRAKGQVHSVLDDIKGVGPKRRKELMKYFKDLGQIKTATVTELLNVPGMNQST
                     AESIYGFFHDSKQ"
BASE COUNT          338 a          321 c          183 g          451 t
ORIGIN      
        1 gacggtgtcc tcttcggatc aactctaaag ctgcttcact cttaccgatc ccactctctc
       61 ctgtgatcag gattccttca ccaaatacat cgactaaaac tccatggatc gtaatgcttg
      121 gagccaatac agttcccagc cagcggatta cttctgccat aaattcagat gtcgtatcct
      181 gagatatcaa aaccggtacc ccatactctt tcgcaacttt tatcatcatc tcacttggat
      241 gcagattcct gcaaaatacg ataactggaa ttcccgctcc gacaaatctt ttataaatat
      301 ttttcgcatt ttcaacatct ttttctacct cttcaataaa aaggtgttct gcttttccga
      361 tgatctgaac tcgattctgt gcaaaatttt caaaaaaacc tgccaactgt aatgctggac
      421 ggttgatctc tgcctgttca atgtaaactt cttttgaatt gatctctggt gttacttctt
      481 ttaaatttaa ttccttgacc atatcatata ctgatactct atgctcttta tccataatct
      541 ttctctcttt ctgcaccctt gattgtctgt ctctttctct attttaacat atttccttac
      601 tgcttggaat cgtgaaaaaa cccataaata gactctgcag tactttgatt catgccagga
      661 acatttaaaa gttccgtaac tgttgctgtc ttgatctgtc caagatcttt aaaatacttc
      721 attaattctt ttcgtctctt tggtccaaca cctttgatgt catcaagaac agaatgtact
      781 tgcccttttg cacgcaatga cctgtgatat tcgatcgcaa aacgatgggc ttcatcctgt
      841 atccttgtca caagtaaaaa tgcttcgctg tttggcgcaa atatgatttc ctcgttgtta
      901 taatacaagc ctctcgttct atgatcatca tctttgacca ttccacatac tggaatatca
      961 agtcccaaat gatccagcac acgaagtgca acattgacct gtccttttcc tccatccatc
     1021 atcagaatat ccggaaattt tgtaaaactg ccaagtgtag tgtcttttcc ttgttctttc
     1081 agttcttcca gctctttctt tccatgaaga aatcttctgg tcaaaacttc ttccatactg
     1141 gcatagtcat ccggaccttc aatggttctt aaacgaaact tgcgataatc tcttcttctt
     1201 gcttttcctc tttcaaatac gaccatggat gctacggtct gaaatccact gatattagaa
     1261 atatcaaatg cttccattcg attcaaacct tcc
//
LOCUS       DAOMUE010000399         1287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_28093_length_1287_cov_2.180195, whole genome shotgun sequence.
ACCESSION   DAOMUE010000399 DAOMUE010000000
VERSION     DAOMUE010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28093_length_1287_cov_2.180195"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="PLC71_11445"
     CDS             <1..459
                     /locus_tag="PLC71_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRM03291.1"
                     /translation="ASSGGTDVIAMILKKYTSVDIGTGLMISDLIFTLAGFLVFNVKT
                     GLYSLFGLIMRSALIDNFIESFNRSKYFHVVTSNATCICDFIQNDLQRGATIVNATGA
                     FTGDDKYIILTVLSPSQAVKLRNFIKEHDPKAFLLVSNTSEIIGKGFHSV"
     gene            600..791
                     /locus_tag="PLC71_11450"
     CDS             600..791
                     /locus_tag="PLC71_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03292.1"
                     /translation="MNNKKKRELTNEEIDENIFEMCNICSTTDCTGLIQVVPRTEAEL
                     ESYEELYPFRPPFLPRDDD"
     gene            complement(982..>1287)
                     /locus_tag="PLC71_11455"
     CDS             complement(982..>1287)
                     /locus_tag="PLC71_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005539805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HRM03293.1"
                     /translation="ETKKAERFFKERGIKYQFVDMKKKGMSKGEFNSVAQANGGLDHM
                     INWEGKDQNLLALIKYIANEDKLEKVLENPQVIKTPVVRNGKQSTLGYQPDVWKKWN"
BASE COUNT          361 a          249 c          209 g          468 t
ORIGIN      
        1 gcttctagtg gtggtacaga cgttatcgct atgattctta aaaaatatac gagtgtagat
       61 atcggaacgg gcttaatgat ctctgatctt atctttactc tcgcaggatt tctagtgttt
      121 aacgtcaaaa caggacttta ttctttgttt ggtctgatca tgcgttctgc gttgatcgat
      181 aactttattg aaagttttaa ccgttctaaa tactttcatg ttgtaacttc caatgcaaca
      241 tgtatttgtg attttatcca aaatgatcta cagcgcggag caaccattgt aaatgcgacc
      301 ggagccttta caggggatga taaatatatt atcctgacgg ttcttagtcc ttcacaggca
      361 gtgaaacttc gaaactttat caaagaacat gatccaaaag cttttctttt agtttctaat
      421 acaagtgaga ttatcggaaa aggcttccat tctgtctagg atggacagcc ttttctctgc
      481 tttcttctgg tacataatac attgttccta tctcctcgta taatatcttt tattggattt
      541 attttacaaa atatttttct taaattatca catactaaga aacgaggagg tttcaatgca
      601 tgaataataa gaagaaacga gaacttacaa acgaagaaat tgatgaaaat atcttcgaaa
      661 tgtgcaatat ttgttctacc acggattgta ctggattgat ccaggttgtt ccaagaacag
      721 aagccgagct tgaatcttac gaagaattgt accccttccg accaccgttt ttacctcgtg
      781 atgatgatta agtcgtcatc cattttctcc tgtaacaaga tcttatggcc tgttatgact
      841 ataaggtctt gtttcaatgt ccactttctc ttctaattag tctgacaaaa aatatcatct
      901 cttattgatt ctaacataat tgtttgtggt gtagcatcat aattttcatc catccaagct
      961 acagactcat cgtccaaata atcaattcca ctttttccaa acatctggtt gatatcctag
     1021 tgttgattgc tttccatttc gaacaactgg tgtcttgatg acttgtggat tttctaatac
     1081 tttttcaagt ttatcttcat tggcaatata tttgatcagt gcaagcaagt tctgatcttt
     1141 gccttcccaa ttgatcatat gatctaaacc accatttgcc tgagctactg aattgaattc
     1201 gcctttgctc atgccttttt tcttcatatc aacaaactga tatttgattc cacgttcttt
     1261 gaaaaaacgt tctgctttct ttgtttc
//
LOCUS       DAOMUE010000400         1269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_28579_length_1269_cov_2.118616, whole genome shotgun sequence.
ACCESSION   DAOMUE010000400 DAOMUE010000000
VERSION     DAOMUE010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28579_length_1269_cov_2.118616"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..348)
                     /locus_tag="PLC71_11460"
     CDS             complement(112..348)
                     /locus_tag="PLC71_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRM03294.1"
                     /translation="MDTNFIISLDSIADVNAFANTIAHFKSEIDLSSGRYIVNAKSIM
                     GIFSLDLTRPLTLTVHGKDELEEISSASAPYLKK"
     gene            467..1072
                     /locus_tag="PLC71_11465"
     CDS             467..1072
                     /locus_tag="PLC71_11465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRM03295.1"
                     /translation="MTEKQKMLMGILYNAEDQALIEERNHAKSLTRQFNEHWEDKGRR
                     NYLIGQIFGSLGKNVHLEAPIYLDYGYRTTIGSDFFSNFNLTILDGGGVEIGNHVFIG
                     PNVGIYTANHPADVKRREKGYEWALPVKIGDKVWIGGGVTILPGVTIGDNSVIGAGSV
                     VTKDIPANVVAAGNPCRIIKEAEEGDRYGIIDEKNGQQSIK"
     gene            1029..1208
                     /locus_tag="PLC71_11470"
     CDS             1029..1208
                     /locus_tag="PLC71_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03296.1"
                     /translation="MGLLTRKTDSNPLSKNIDELLDGMNQEREETKEGTYEESLKKIE
                     ERIKELGILEEENED"
BASE COUNT          447 a          183 c          295 g          344 t
ORIGIN      
        1 gcatgattta agaatgaact tagatcatgc tttttttctt ttaccaaata caaaataaaa
       61 gaagcctcta aaaacagaga cttcaaatgc tcgcattgat tacaagtaaa cttatttttt
      121 caagtatggt gcacttgcag agctgatttc ttcaagttca tctttaccat gtacggttaa
      181 agtcaaaggt cttgtcagat caaggctgaa aattcccatg atcgattttg catttacgat
      241 atatcttcca gaagaaagat caatctcaga tttaaaatgt gcgatcgtat ttgcaaatgc
      301 attcacgtct gcaatggaat caagagaaat aataaagttg gtatccatgt ttaaacctcc
      361 ttaatggtta aatctctttt atcctacaat ttttttgata gaaagtcaaa ttcgatagat
      421 aacagaaaag aagtatgata aaatataaga caacggaggg aaaaagatga cggaaaaaca
      481 aaaaatgttg atgggaattt tatataatgc agaggatcaa gctctgatag aagagaggaa
      541 ccatgcaaaa tctctaacca gacagtttaa tgaacattgg gaagataaag gacgtcggaa
      601 ttatctgata ggacagatat ttggaagtct tgggaagaat gttcatctgg aagcacccat
      661 ttatctggat tatggatata ggacaacgat tggaagtgac tttttctcaa actttaatct
      721 gacgatttta gatggtggag gagttgaaat cgggaatcat gtttttatag gtccgaatgt
      781 aggaatttat actgcgaacc acccagcaga tgtgaagaga cgagaaaagg gatatgaatg
      841 ggccctccca gttaagattg gagataaagt atggattggc ggaggggtta cgattcttcc
      901 tggagttacg atcggcgaca atagtgtgat cggtgcagga agtgttgtaa caaaggatat
      961 tccagccaat gtagttgctg caggtaatcc atgcaggatc atcaaagaag cggaagaagg
     1021 agacagatat gggattattg acgagaaaaa cggacagcaa tccattaagt aagaatattg
     1081 acgaattact tgatggaatg aatcaggaga gggaagagac aaaagaagga acttatgaag
     1141 aatctttaaa gaagattgag gagcggatca aagaacttgg gattttagaa gaagagaacg
     1201 aagattaaga aaggcatcgg tggtgccttt ctttttttag aagtgcgccc ggcggcgcac
     1261 gtttctagc
//
LOCUS       DAOMUE010000401         1267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_28663_length_1267_cov_1.514851, whole genome shotgun sequence.
ACCESSION   DAOMUE010000401 DAOMUE010000000
VERSION     DAOMUE010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28663_length_1267_cov_1.514851"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLC71_11475"
     CDS             complement(<1..309)
                     /locus_tag="PLC71_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRM03297.1"
                     /translation="MKKRSKEILDLLGRRENDHTISTLAAKFEVSERTIRNDIKDIND
                     YLKEQEIKPIRFGSNGLIVMEEDISQARETTQQEDFYTYKLSKEERRMLAAAILINTK
                     D"
     gene            complement(373..>1267)
                     /locus_tag="PLC71_11480"
     CDS             complement(373..>1267)
                     /locus_tag="PLC71_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRM03298.1"
                     /translation="SPIISGSSTAKASGNSSSVTLKGITEEYEDIQNISVQKGRYILD
                     VDNDNRLQVCLIGVTAAKDLFGHTDVEGETIRISGRNFKIIGVLEEDGSSQRDSNDSV
                     IYTPFTTAQRIMQNSTISSFYVSATNEGMLNMAESVVDSFLLEKTGDEDSYTITNQSD
                     IADSVSDVTNSMTYMIGGIAGISLLVGGIGIMNIMLVSVTERTKEIGIRKAIGAKKKD
                     ILAQFMIESTVLSCMGGVIGIALSAATIFGMNQLMSADYTISAGISLIALAFSAILGI
                     VFGLYPANKAANLKPIEALNL"
BASE COUNT          308 a          267 c          225 g          467 t
ORIGIN      
        1 atctttcgta ttgatcaaaa ttgctgctgc aagcattctt cgttcttctt ttgatagttt
       61 gtatgtataa aaatcttcct gctgtgttgt ctctctcgcc tgacttatat cttcttccat
      121 cacgattaag ccgttacttc caaaacggat cggtttgatc tcttgttctt tgaggtagtc
      181 attgatatct ttaatatcat tacgaatcgt gcgttctgaa acctcaaatt ttgctgctag
      241 tgttgaaatc gtgtgatcat tttctcttcg gcctaacaaa tctaatattt cttttgatcg
      301 ctttttcata tgattttgcc tcctgtgtaa tgtatatatt ataatacatt acgcaagagg
      361 cttcaaagta ttttataaat tcaacgcttc gattggtttt aaattcgctg ccttattagc
      421 aggatataat ccaaacacga ttccaagtat cgctgaaaat gctaatgcga tcagactaat
      481 tcctgctgat atcgtataat ctgctgacat taactgattc attccaaaaa tggttgctgc
      541 acttaatgcg attccgatca cacctcccat acaggacaga acggttgact cgatcataaa
      601 ttgagccagg atatctttct tcttcgcccc gatggctttt cggattccga tctcttttgt
      661 tcgttctgta acggacacaa gcatgatgtt catgattccg atcccaccta ccagaagtga
      721 gattcctgcg attcctccga tcatatatgt catggagttt gtaacgtcgc tgacgctgtc
      781 tgcgatatca gactgatttg tgatcgtata ggaatcttca tctcctgttt tttctaacag
      841 gaagctgtct actacacttt ctgccatgtt aagcattcct tcatttgttg cagatacata
      901 aaaactgctg atcgtgctgt tttgcatgat cctttgtgct gttgtaaatg gagtatagat
      961 tactgaatcg ttggagtctc tctgggatga tccatcttct tctaagactc cgatgatctt
     1021 gaagtttctt cctgagattc ggatcgtttc tccttctaca tcggtatgtc caaataagtc
     1081 tttggctgct gtgactccaa tgaggcagac ttgcagccgg ttatcattat ctacatctaa
     1141 gatataacgt cctttttgta cactgatatt ttgaatatct tcatattcct ctgtaattcc
     1201 ttttaatgtt acactggatg aatttccaga tgcttttgct gtagagcttc ctgaaatgat
     1261 cggtgat
//
LOCUS       DAOMUE010000402         1255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_28973_length_1255_cov_1.420000, whole genome shotgun sequence.
ACCESSION   DAOMUE010000402 DAOMUE010000000
VERSION     DAOMUE010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28973_length_1255_cov_1.420000"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="PLC71_11485"
     CDS             complement(<1..667)
                     /locus_tag="PLC71_11485"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HRM03299.1"
                     /translation="MKKAKKEKGVKIMGYVYVGCRTTKERNARGEGLKVYEVSEVDGR
                     WTEIQCLKTEENPSFQCFDNTKEYLYSVHGDFTLVSSYKILEDGTLEHINTIDIGGKN
                     PVDVTVDAENQHVIVATLQGGTLYTIKRNEDGSLGDVVATFTYEGKEEGKVSTIHQCL
                     WDQKKNYLFACAQGRVNGFGQMRALKYNHETGEFTETDKFLSRTWDEPRHAAMHPNNR
                     WLYM"
     assembly_gap    1027..1036
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1037..>1255)
                     /locus_tag="PLC71_11490"
     CDS             complement(<1037..>1255)
                     /locus_tag="PLC71_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRM03300.1"
                     /translation="FTEDLYIAAPQGVSIDEIEDMPLIKSFLNPMVEMIVDQWWKNQF
                     GARAHESQYVPFIDIAIEMVEKDLGVAF"
BASE COUNT          330 a          281 c          173 g          461 t
ORIGIN      
        1 acatgtaaag ccatctgtta tttggatgca tggctgcatg tcttggttca tcccacgttc
       61 ttgataagaa cttatctgtc tctgtaaatt ctcctgtctc atggttgtat tttagtgctc
      121 tcatctgtcc aaatccatta acacgtcctt gtgcacatgc aaacagataa ttctttttct
      181 gatcccacag acactgatgg atcgtggata cttttccctc ttcttttcct tcgtaagtaa
      241 aagttgcaac gacatctcct aaacttccat cttcatttcg tttgatcgta tagagtgttc
      301 caccttgaag tgttgcaacg atcacatgtt gattttcggc atcaactgtc acatcgactg
      361 ggtttttacc tccgatatca atcgtattga tatgttctaa tgttccatct tccaaaatct
      421 tgtaagaaga taccagtgtg aaatctccat gaacgctgta aagatattct tttgtgttat
      481 caaaacactg gaaacttgga ttctcctctg tctttaaaca ctgaatctct gtccatcttc
      541 catctacttc tgatacttca taaaccttta atccttcccc tctggcgttt ctttcttttg
      601 ttgttctgca tcctacatac acatatccca taattttaac tcctttttct tttttggctt
      661 ttttcatccg tttcatttcc tatgaatcca ttatagttca ttttacatgt caataaaaaa
      721 ttcatatttg gactatctaa gtcattccga aatggaatat atatttttcg caataattat
      781 cggatattgt atacaatatt gatatttttt cgattttcta tcaatctaca aataaaaaaa
      841 gaaccaacat ttttaacgtg ctgatccttc ttttgttatt tttcttcctg atagaatttc
      901 tctacaaaat caataaaatc tacgattgtc tgcgactgat agcatttgtc agaaagcatc
      961 atccagacct ctctcttgac aggctctccc tgcagatcgt atgccggaat taattggact
     1021 ttctctnnnn nnnnnnaaaa atgcaactcc aaggtccttt tctaccatct cgatggcaat
     1081 atcaataaac ggaacatact gcgattcatg cgctcttgcc ccaaactggt tcttccacca
     1141 ctggtctaca atcatctcca ccattggatt taggaaagat ttgataagtg gcatatcttc
     1201 gatctcatca atacttacac cttgtggtgc tgctatatat aagtcttctg taaat
//
LOCUS       DAOMUE010000403         1235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_29571_length_1235_cov_1.718644, whole genome shotgun sequence.
ACCESSION   DAOMUE010000403 DAOMUE010000000
VERSION     DAOMUE010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29571_length_1235_cov_1.718644"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1235)
                     /locus_tag="PLC71_11495"
     CDS             complement(<1..>1235)
                     /locus_tag="PLC71_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009290952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HRM03301.1"
                     /translation="SRHRAVTLSEMEEKYFVSKSTLLNDLKKVEEVLDKFHLELMHGS
                     NKVMVDGFEINKRRCLAEQDLYLAHIKNEHGMSYIDERQLAKIKNILTEKFVEHQYHI
                     MDTDFNNAILFLNIMICRMGDGFYVQSNELDITENLGKEYEMSKDIFEKIGRRFFIKV
                     TDEEIKYFALYLKGQGNNRDSDTITPEMDKFITEAFAQIKENFGVDFTDNINLRITLA
                     LHCMSLQIRIKYDMQMKNDMLDYIRETFPMGYDIGTYFAFLLSQKYGKKVSDDETALL
                     AVHFYSSLMELNSGVGKIKILVISSLKNSMTLLLKQSLLRWFSDRVSAVDFCNPMDVT
                     EDMLDEYDIFLTTEKDEFFEKGLAMYVNAFPNQHDYANIKLNLDGFKDIEDIASIFYP
                     ELFYQVKSSTKKEVIQGLC"
BASE COUNT          378 a          246 c          168 g          443 t
ORIGIN      
        1 cacacaaacc ttgaattact tccttctttg ttgaagattt tacctgatag aataattctg
       61 ggtaaaaaat acttgcaata tcttcaatat ctttaaatcc atctaaattt aatttgatat
      121 ttgcataatc atgctggttc gggaatgcat ttacatacat tgcaagtcct ttttcaaaaa
      181 attcatcctt ctctgttgtc aggaaaatat catattcatc cagcatatct tctgtgacat
      241 ccattggatt gcaaaaatct actgcagata ctctgtcgga gaaccatctg agtaatgact
      301 gttttaataa taatgtcata ctgttcttca acgatgagat cacaaggatt ttgatctttc
      361 caacacctga attaagttcc atcagactgc tgtaaaaatg tactgccagt aatgccgtct
      421 catcatcact gactttcttt ccgtattttt gtgataaaag gaaagcaaaa tatgttccga
      481 tatcatatcc catcgggaat gtctctctga tataatcaag catatcattc ttcatctgca
      541 tgtcatactt aattcgaatc tgtaatgaca tacaatgaag cgctagtgta attcttaagt
      601 tgatattatc tgtaaaatca acaccaaagt tttctttgat ctgtgcaaat gcttctgtaa
      661 tgaatttatc catctctgga gtgatcgtgt ctgaatctct gttatttccc tgacctttta
      721 gatacaatgc aaaatatttg atctcttcat ctgtgacttt aataaaaaat cttcgtccaa
      781 tcttctcaaa aatatctttg gacatctcat attcttttcc aagattttct gtgatatcta
      841 actcatttga ctgtacatag aaaccatctc ccatacggca gatcataatg tttaagaaaa
      901 ggatcgcatt gttaaagtct gtatccataa tatgatattg atgttctaca aatttctctg
      961 tcagaatatt cttaatcttt gccaattgtc tttcatcaat ataagacata ccatgttcat
     1021 tcttaatatg tgcaaggtaa agatcctgtt ccgcaagaca tcgtcgttta ttgatctcaa
     1081 aaccatccac cataacttta ttgcttccat gcattaattc gagatgaaat ttatccagaa
     1141 cttcttccac tttcttaaga tcgtttaaca gtgttgactt agaaacaaag tacttctctt
     1201 ccatctctga aagagttaca gcacgatgtc tgctc
//
LOCUS       DAOMUE010000404         1227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_29791_length_1227_cov_2.224403, whole genome shotgun sequence.
ACCESSION   DAOMUE010000404 DAOMUE010000000
VERSION     DAOMUE010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29791_length_1227_cov_2.224403"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..>1227)
                     /gene="aroA"
                     /locus_tag="PLC71_11500"
     CDS             complement(16..>1227)
                     /gene="aroA"
                     /locus_tag="PLC71_11500"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRM03302.1"
                     /translation="SHRAVMFGSLAKGTTHISNFLSGADCLSTIDCFRKMGVLIEQDG
                     TNVTVHGNGLHGLKAPSETLDVGNSGTTTRLISGILAGQDFETVLSGDASLNKRPMGR
                     IIKPLEQMGAKITSVNGNGCAPLKIEGTKLNATHYDSPVASAQVKSCVLLAGLYADGK
                     TSVTEPALSRNHTELMLRSFGVKVESHDTTATITPPEEMIATDIVVPGDISSATFFIV
                     AGLITPNSCIRLKNVGINPTRDGILRVCKDMGADVTLENVIDNGGEPTADIVVKTSKL
                     KGTVIGGEVIPTLIDEIPVIALLAAFAEGETVIKDAAELKVKESDRIALTVDNLVKMG
                     VDATATDDGMIIKGGNPLHGASINCKYDHRIAMTFSIAGINAEGETVIEDSECVDVSY
                     PTFYEQLNSLQ"
BASE COUNT          317 a          288 c          198 g          424 t
ORIGIN      
        1 atcatagcat tttatttatt gcaatgaatt taactgttca taaaatgttg gataggacac
       61 atccacacat tctgaatctt cgatcacagt ttctccttct gcattgattc ctgcaatcga
      121 aaatgtcata gcgattcgat ggtcgtattt acaattgata cttgctccat gtaatggatt
      181 accgcctttg atgatcattc catcgtctgt cgctgtcgca tcaacaccca ttttcacaag
      241 attatcaacc gttaatgcga ttcgatcaga ctcttttacc ttcaactctg ccgcgtcttt
      301 gatcactgtc tcaccttctg caaatgctgc taacagtgca ataactggaa tctcatcgat
      361 caatgttgga atgacttctc caccaatcac cgttcctttt aatttgcttg tctttacaac
      421 gatatctgct gttggctctc ccccattatc gattacattt tctaatgtaa catcagctcc
      481 catatcttta catacacgca ggattccatc tcttgttgga ttgattccaa cattttttaa
      541 acggatacat gaatttggtg tgatcagtcc tgccacgata aagaaagttg ctgaagaaat
      601 atctcctgga accacgatat ctgttgcgat catctcttct ggaggtgtga tcgtcgctgt
      661 tgtatcatgt gattctactt tcactccaaa agaacgaagc attaattctg tatgatttct
      721 tgataatgct ggttctgtca cacttgtctt gccatctgca tataatcctg ctaataacac
      781 acaggatttt acctgtgcag atgcaactgg agaatcataa tgtgttgcat ttaattttgt
      841 tccttcaatc ttcaatggtg cacatccatt tccattcaca cttgtgatct ttgcacccat
      901 ctgttctaat ggtttgatga tccttcccat tggacgttta tttaaggatg catctccaga
      961 taatacagtt tcaaaatcct gacctgccag gattcctgag atcaatcttg ttgttgttcc
     1021 gctatttcct acatctaatg tctcacttgg tgcctttaag ccatgaagtc catttccatg
     1081 aacagtgacg ttcgttccat cctgttcgat caaaacaccc atcttacgaa aacaatcgat
     1141 cgttgacaga cagtctgctc ctgataagaa attgctgata tgtgttgttc ctttcgctaa
     1201 agatccaaac ataactgctc tatgact
//
LOCUS       DAOMUE010000405         1202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_30612_length_1202_cov_1.464690, whole genome shotgun sequence.
ACCESSION   DAOMUE010000405 DAOMUE010000000
VERSION     DAOMUE010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30612_length_1202_cov_1.464690"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>259)
                     /locus_tag="PLC71_11505"
     CDS             complement(<1..>259)
                     /locus_tag="PLC71_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRM03303.1"
                     /translation="VHIPESSEMDAYVEDGFEVKMRDVDFSYIEGNRVITDSAFMANP
                     GEIVALVGPSGEGKTTMIRLILGLIHPEKGEAYLRASNGKQV"
     assembly_gap    260..269
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<270..>527)
                     /locus_tag="PLC71_11510"
     CDS             complement(<270..>527)
                     /locus_tag="PLC71_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM03304.1"
                     /translation="EKFKDISLKYNMFTIKTNIVMSIIGMVVQYAAFGYCLFRLWTRD
                     ISYGTMTLFLQQRSNLNGAFNNLVSIIPNFLNSSISAHRIR"
     assembly_gap    528..537
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<538..>1202)
                     /locus_tag="PLC71_11515"
     CDS             complement(<538..>1202)
                     /locus_tag="PLC71_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRM03305.1"
                     /translation="EIIDDWKWIFSYSKRYKGAIAFYTILGIFSTSMGLVGSVASKYM
                     IDIITGYQMSKLWIMVLITLGSAFFSLTIGNVIGRISTKLSIYINNDIQADIFDKIID
                     ADWMEINQYSNGDVLNRFNSDVNTISGNAISWLPTIIIAVYDFVATFAVIWHYDKIMS
                     LLAFASAPFMLLMSKFLISKQREYGKKVREMSSEMMTFEVETFYNFDTIKSFGISSLY
                     GKM"
BASE COUNT          369 a          285 c          180 g          348 t
ORIGIN      
        1 ccacctgctt tccatttgaa gcccttaaat atgcttctcc tttttccgga tggatcaaac
       61 ctaagatcaa acggatcatc gttgtctttc cctctccaga aggaccaacc aacgcaacga
      121 tctcgcccgg attagccata aatgcagaat ctgtaataac acggtttcct tcgatataag
      181 aaaaatccac atcccgcatc ttcacctcga acccatcttc cacataagca tccatctcag
      241 agctttctgg aatatgaacn nnnnnnnnnc tctgatacga tgagcggaga tcgaactgtt
      301 caaaaagttt ggtatgatcg ataccagatt gttaaatgcc ccatttaagt tacttctctg
      361 ttgtaaaaat aaagtcatgg ttccataaga aatatctctt gtccaaagtc ggaacagaca
      421 atatccaaat gctgcatact gaacaaccat tcctatgatc gacataacga tattcgtctt
      481 gatcgtaaac atgttatact ttaaactgat atctttaaac ttctcctnnn nnnnnnncat
      541 ctttccatat aaagaagaga tcccaaaaga cttgatcgta tcaaaattat agaatgtctc
      601 gacttcaaat gtcatcatct cactactcat ttcccgcact ttcttcccat attctctctg
      661 cttggaaata aggaacttcg acatcaaaag cataaatggt gcacttgcaa atgccagtaa
      721 tgacatgatc ttatcgtaat gccagataac cgcaaatgtt gcgacaaagt cgtaaactgc
      781 tatgatgatc gtcggcagcc agctgatcgc atttccactg atcgtattga catcgctgtt
      841 aaaacgattc aaaacatctc cattactata ttgattaatc tccatccagt cggcatcgat
      901 gatcttatca aaaatatccg cctgaatatc attattgata tagatactca actttgtaga
      961 aatacgtccg atcacatttc cgatcgtcag actaaaaaat gcacttccaa gtgtgatcaa
     1021 gaccatgatc cacaacttac tcatctgata tcctgtgatg atatcgatca tatatttact
     1081 tgccacactt ccaaccaatc ccatggatgt gctgaaaata ccaaggatcg tgtagaaagc
     1141 gatcgctcct ttatatcgtt tgctgtagga aaagatccat ttccagtcgt caatgatctc
     1201 tg
//
LOCUS       DAOMUE010000406         1199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_30680_length_1199_cov_2.944930, whole genome shotgun sequence.
ACCESSION   DAOMUE010000406 DAOMUE010000000
VERSION     DAOMUE010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30680_length_1199_cov_2.944930"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1102)
                     /locus_tag="PLC71_11520"
                     /pseudo
     CDS             complement(1..1102)
                     /locus_tag="PLC71_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202770.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
BASE COUNT          380 a          288 c          183 g          348 t
ORIGIN      
        1 ccatacgtta aatcctagag cacatggtaa ggataacaca ataatgagta ctgcattaaa
       61 tacgatcgct ttcttaaggc tccatcccca taggtcttgt gcaaatgaca ggatgttctg
      121 aaatactgca atgatcgttg atagtgccgc aaagctcata aataagaaga acatcgctcc
      181 ccagatcctg cttcctggca tttcgttaaa tacatttggc agtgtgataa atactaagtt
      241 tggtccgctt cctggattta ctccgaatgc aaaacatgct ggaaaaatca ccagtcctgc
      301 cattaatgct actaacgtat caagaattgc cacactgatt gcttctcctg tcagtcttcg
      361 gctcttgtca atgtaacttc caaagattgc aatcgcccct attccaagac ttagtgtaaa
      421 gaatgcctgt cccattgctg cggatacgac cgtcatcacc ttgtttcttc actgctgcaa
      481 aatctggaat cagataaaat tttaatcctg cagaggctcc atctaaagtt acactccttg
      541 cgatcaatac cagaattacg ataaacaaac atgacatcat aactgttgtg atcttctcta
      601 ctccattttg aagaccaaga gaacatacta aaaatcccaa tgcaataact actaacatcc
      661 agaagatctg tgcctttgga tctgacagca ttgttccaaa agtattttct acctgtgctg
      721 gatttttccc aacaaagtct ccttttaaca tcttaaagaa atagctgatc atccatcctg
      781 ctactgtcgt gtaaaacatc atcaacagat aatttccagc catgccaaca taactgtacc
      841 aatgccattt tgttcctttc ggttctaaca cctgaaagga tcttgcaata ctcttctgac
      901 tggctcttcc gactgcatat tccataacaa ggattggcag ccctaaaatg atcaggaaga
      961 acagatagat cagtacaaat gcagctccac cgtattgtcc tgtgataaat ggaaatctcc
     1021 acacatttcc caagccgatc gcacaaccag ccgagatcaa aagaaatcca attctagatg
     1081 aaaatttctc tctattttcc aaattacttt cctcttttct atatattttg actcacaaaa
     1141 gtttattgta ccatagcttg gcaatattta aaaccaaaaa aacaatttca caaaagact
//
LOCUS       DAOMUE010000407         1194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_30876_length_1194_cov_1.601405, whole genome shotgun sequence.
ACCESSION   DAOMUE010000407 DAOMUE010000000
VERSION     DAOMUE010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30876_length_1194_cov_1.601405"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1194)
                     /locus_tag="PLC71_11525"
                     /pseudo
     CDS             complement(46..1194)
                     /locus_tag="PLC71_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393439.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT          335 a          277 c          133 g          449 t
ORIGIN      
        1 tgtaatctta tccatttccg gaaaaattgc ttcatattat cttatctatt ttctttttac
       61 catacatctc tttttgtaaa aagaaatccc ataacagatc acctcatcat aactatcctc
      121 aaattccttt gcatacatcc tatgattgat ctgctgaata gctttttcgc aatcctcttc
      181 taatttcttc acttcttttg aatatttcgc ttcaaaaact gcaacctttc cttcacattc
      241 attaaaaata acaagatcac ttcgaccttc tccatgttct ttattcgatt caacactata
      301 tccagcccca gcaaatattc ctgcaagaaa tgcatgataa aaatcttctc tataatcatg
      361 ataactgatc gttattcgca acaacctgct gatctcctgt gtcaaagtct gctcatcacc
      421 gttccatact gcatcaaata aatgctttcg atcttgtagt ttcgctgtat cactaaacca
      481 atcctgtact gttgtctcaa aaatctctcg tatctctttg tttggaatct tcaatgtaat
      541 ctttccatct gcactttcct tctcttcctt atctcttgtc agatatcctg ttaaatacaa
      601 aatactccaa aaattctctt ctgacgaatg taaataatca tacgtaagat tttcttctat
      661 tttctgatca atcgtgtctc ctgccatcaa tttttccatc tttttttgaa ttccgctgcc
      721 tgcgtagtct ataaaagatc gttccagtaa cttgctggct tcatgtctgg atcgatccgt
      781 aaatctctta catagttcat aacatcccat ggacaatata catcgatctt tccaaaatta
      841 tacccatcat accattcttt gatttcttta aaatgctctt gcagtccaat atcttttaag
      901 atatctttta cttcctgatc ggtaaatcca aaatattcat ttaaatgtgt tgctatgatc
      961 gtatctgaga taaaattatt ggttcctgta aatatacttt cctttgcaat tttcaagcaa
     1021 cctgtaacta ctgcgaactg aagatgagaa ttatctttta gagatgtgct catcatcgtt
     1081 ttcatcacat ccagcatttc tttatagtag ccattgctgc ttgctttcgc taatggtaca
     1141 tcatattcat ccaataataa aataaccggc tttccgtaat gcgcattcat catt
//
LOCUS       DAOMUE010000408         1155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_32154_length_1155_cov_2.061818, whole genome shotgun sequence.
ACCESSION   DAOMUE010000408 DAOMUE010000000
VERSION     DAOMUE010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32154_length_1155_cov_2.061818"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1155
                     /locus_tag="PLC71_11530"
     CDS             <1..>1155
                     /locus_tag="PLC71_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRM03306.1"
                     /translation="DSYSDYIGKIETTLIRDLKKEYPQIEVYEDDSAADVLETIFEEC
                     NQERFVFVLDEWDFIFHKDFINEENKKQYIQFLSNLLKDRPYVQITYMTGILPIAKYS
                     DGSELNMFDEYDMACKEKYSEYFGFLDEEVDQLYDIYKKITKNPKILRSELKEWYDGY
                     RAAAGDYLYNPRSVVCALRDNQLANYWTSSGTYDSIFNYIKGNIADVLNDLVLMVAGE
                     HVPAKMQQYAATANVLHTKDQIYSAMVVYGLLTYENGKVFIPNKELMDKFDELLLSNQ
                     QLGYVYRLARESQRMLQATIHGDTDTMEEILEYVHDTEVPIYSYNSEVELSAIVNLVY
                     LSARDEYRVEREDKAGKGFVDFIFYPKRNNIPGIILELKIDHTPQKAIEQI"
BASE COUNT          421 a          154 c          238 g          342 t
ORIGIN      
        1 gactcatatt ctgattatat aggaaaaatc gagacaactt tgatcagaga tcttaagaag
       61 gaatatccac agattgaagt gtatgaagat gattcggcag cggatgtatt agaaacgatt
      121 tttgaagaat gtaaccagga acgctttgtt tttgttctgg atgaatggga ttttattttt
      181 cataaggatt ttattaacga agaaaataaa aaacagtata tccagttctt aagtaattta
      241 ttaaaagacc gtccatatgt tcaaataact tatatgacag gaatccttcc aatcgcaaaa
      301 tattctgatg gatcagaatt aaatatgttt gatgagtatg atatggcatg taaagaaaaa
      361 tacagtgaat attttggctt tttggatgaa gaagttgacc agctttatga catttataaa
      421 aagataacaa agaatccgaa gattttaaga agtgaattaa aagaatggta tgacggttat
      481 cgggcggcag caggagatta tttgtataat ccaagatcgg ttgtttgtgc attaagagat
      541 aaccaactgg caaactactg gacaagttct ggaacttatg attctatttt caactatatt
      601 aaagggaata tagcagatgt gctaaatgat cttgtgctaa tggtcgcagg agaacatgtt
      661 ccagcaaaaa tgcaacaata tgcagcaaca gcaaatgtgt tgcataccaa agaccagatt
      721 tattcagcaa tggtcgttta tggattgttg acctatgaga atgggaaggt atttattccg
      781 aacaaagagt tgatggacaa atttgatgaa ttgctgttat caaaccagca attaggatat
      841 gtttaccgat tggcaagaga atctcaacga atgcttcagg caacaattca tggggatacg
      901 gatacaatgg aagagattct tgaatacgtc catgatacag aagttccgat ttattcatat
      961 aatagcgaag tggaattatc agcaattgta aatttagttt acctctctgc aagagatgaa
     1021 taccgtgttg agagagaaga caaagcagga aaaggatttg tggattttat cttttatcca
     1081 aaaagaaata atatacctgg aattattctg gaacttaaaa tagatcatac accacaaaaa
     1141 gcgatagagc agatt
//
LOCUS       DAOMUE010000409         1124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_33261_length_1124_cov_2.377923, whole genome shotgun sequence.
ACCESSION   DAOMUE010000409 DAOMUE010000000
VERSION     DAOMUE010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33261_length_1124_cov_2.377923"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLC71_11535"
     CDS             complement(<1..437)
                     /locus_tag="PLC71_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRM03307.1"
                     /translation="MKKYSEKIMHVVFLIAACASILAVILICAFLFVNGIPTIGKIGL
                     GNFVLGKIWMPKSGIFGIFPMIIGSIYVTAGAILVGVPVGILAAIYMAKFCNEKVYKI
                     VKPAVDLLAGIPSVVYGFFGMVVLVPAFRNIFGNGNCVFTASI"
     gene            complement(470..>1124)
                     /locus_tag="PLC71_11540"
     CDS             complement(470..>1124)
                     /locus_tag="PLC71_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRM03308.1"
                     /translation="ITNSTSVMMTTVAENKAAIGYISLGSLNDTVKAVKIDGAEASVD
                     TVKDGSYKIVRPFNIVTKDKLSKQAQDFENYIMSADGQKIIQDNGYIKADEKAPAYKS
                     NGAKGKVVVGGSSSVTPVMEKLKEAYAKANKDVTVEVQQSDSTTGVTNAIEGTCDIGM
                     ASRDLADSEAKKGVKATVIAKDGIAVIVNKDSKVDELTSAQVKDIYTGKTTKWADIK"
BASE COUNT          307 a          244 c          162 g          411 t
ORIGIN      
        1 aaaatagatg ctgtaaatac acagttacca tttccaaata tattacggaa agctggtact
       61 aacactacca ttccaaagaa tccataaaca accgatggaa ttcctgccag aagatcaact
      121 gctggtttta ctattttata cactttttca ttacaaaact ttgccatata gatcgcagca
      181 agaattccaa ctggaactcc aaccaaaatc gctcctgctg ttacataaat acttcctatg
      241 atcatcggga aaatgccaaa gattccactc tttggcatcc agatcttacc taaaacaaaa
      301 tttccaagtc cgattttccc gattgtagga ataccattta cgaacagaaa tgcacagatt
      361 aaaattactg ccaaaattga ggcacatgct gcgatcaaaa atacgacatg catgattttt
      421 tctgaatatt tcttcattat taccattcct ttatattgat gattctttct tatttaatat
      481 ctgcccattt tgttgttttt cctgtgtata tatcttttac ctgagcactt gttaattcat
      541 caactttact gtctttattt acgataactg cgataccatc ttttgcgata actgttgctt
      601 ttacaccttt tttagcttcg ctgtctgcaa gatcacgaga tgccataccg atatcacatg
      661 ttccttcgat cgcatttgta acacctgttg ttgaatcgct ctgctgcact tcaactgtta
      721 catctttgtt tgcttttgca tatgcttctt ttaatttttc cataactggt gttactgaag
      781 aagatccacc aacaacaact ttaccttttg ctccgtttga tttgtaagct ggtgctttct
      841 cgtctgcttt gatatatcca ttatcctgaa tgatcttctg tccatctgca ctcatgatgt
      901 agttttcaaa gtcctgagcc tgtttgctta atttatcttt tgttacgata ttaaatggac
      961 gaacgatctt gtatgatcca tcttttacag tatcaacact tgcctcagct ccgtcgatct
     1021 taactgcttt tactgtgtca tttaaagatc ctaaagatat gtatccgata gctgctttgt
     1081 tttctgcaac tgttgtcatc attacagatg ttgagtttgt gatc
//
LOCUS       DAOMUE010000410         1121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_33383_length_1121_cov_1.701689, whole genome shotgun sequence.
ACCESSION   DAOMUE010000410 DAOMUE010000000
VERSION     DAOMUE010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33383_length_1121_cov_1.701689"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..902)
                     /gene="gatY"
                     /locus_tag="PLC71_11545"
     CDS             complement(48..902)
                     /gene="gatY"
                     /locus_tag="PLC71_11545"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HRM03309.1"
                     /translation="MPLVTTKQMLLDAQAGNYAVGAFNVENMEMVMAVMEAAEELKSP
                     VIMQTTPSTVKYAGLDFFLANVKAAAERASVPVAMHLDHGSSFELAMQAYRTGYTSIM
                     IDGSHGSFEENVAVSKAVADACAPSGIPVEAELGKVGGKEDDLDGGDDNPYTDPAQAV
                     EFVKQTGVTSLAVAIGTAHGVYKGEPKLDLDRLSEIRKVVDIPLVLHGTSGVPDATVT
                     ECVNRGICKVNYATDLRIAFTKGVEKVFEENPDVIDPKKYNAAGKECVKEYVMSKMKV
                     CKSVGKAN"
BASE COUNT          260 a          256 c          178 g          427 t
ORIGIN      
        1 ttgcgtgtac aactcctgct ttcatgctta ttctccttcc tgatgtatta gtttgctttt
       61 cctacactct tgcatacttt cattttggac ataacgtact ctttgacaca ttcttttcct
      121 gctgcattgt attttttagg atcaatgaca tctggatttt cttcaaatac tttttcaact
      181 ccttttgtaa atgcaattct taaatctgtt gcataattta ctttacagat tcctcgattt
      241 acacactctg taacggttgc atctggtaca ccgcttgttc cgtgtaatac taatggaata
      301 tcaacaactt tacgaatttc tgataatcga tctaagtcaa gttttggttc tcctttgtat
      361 acaccgtgcg ctgttccgat tgccacagct aaggatgtaa ctcctgtttg ttttacaaat
      421 tcgacagcct gtgctggatc tgtatatggg ttgtcatctc caccatctaa gtcatcttct
      481 tttcctccaa ctttaccaag ttctgcttca actggaattc ctgatggtgc acatgcatct
      541 gcaactgctt tggatactgc aacattttct tcaaaacttc cgtgagatcc atcgatcatg
      601 atagatgtgt atcctgttct gtatgcctgc attgctaatt caaaactgct tccatggtct
      661 aaatgcatgg ctactggcac gcttgctctt tctgctgctg cttttacatt tgctaggaag
      721 aaatctaatc ctgcatattt aactgtagat ggtgttgtct gcatgataac tggagatttt
      781 aactcctctg ctgcttccat aaccgccatg accatttcca tattctctac attgaaagcc
      841 ccaactgcat aatttcctgc ctgggcatct aataacatct gttttgttgt aactaaaggc
      901 atctcttttt cctcctctaa agttcggggc agactttttt aagtccgccc gttcatatat
      961 ttacactttt atatcattgt ctgcttgttt ttaatattcg ctttctttta aagctgctgc
     1021 tctctgttta gatccacgaa ctgtaaagat catgcaaatc acataaatga ttccgataat
     1081 taccagtctt ttgatgttct gctgtgtcac gatgtgtgta t
//
LOCUS       DAOMUE010000411         1117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_33547_length_1117_cov_1.660075, whole genome shotgun sequence.
ACCESSION   DAOMUE010000411 DAOMUE010000000
VERSION     DAOMUE010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33547_length_1117_cov_1.660075"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..>1117)
                     /locus_tag="PLC71_11550"
     CDS             complement(174..>1117)
                     /locus_tag="PLC71_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRM03310.1"
                     /translation="VVNILLVGSDNGAQGSEKGDHGRSDSMMVATINFKTKELKLTSF
                     LRDMYVEIPGHGRNKLNAAYAFGGEALLYQTLAQNFNIKIDKFCVVDLAAFEKVINRI
                     GGIEMTLEQREAEYLNTTNYISKKKYRNVKVGKQTLNGNQALGYARVRHVFSKKYGVE
                     EFGRTGRQRAVMQATFQKMLQQNPLDLVDIAIDALGDVSTDMDATYIKKLILSVAKMG
                     TTEIDQLRVPIENTYKTAVAGSYPPCGYVFFVNFKANQEALKYFMFNKGKRQDFAKNY
                     GGADAAETCGYPSKYDYNRSKGDSGNIFNSSDTTEGE"
BASE COUNT          326 a          238 c          162 g          391 t
ORIGIN      
        1 taaatccatg gttttttcct cctgtatgat ataaatatgt atttatttct cgtatactta
       61 agagctatat gactttatat gctctctaag taactttgtc cattataaaa catcctatgg
      121 cgattactca agactaatcg cagtgcctta ttgataatat ttctcaataa gcgctactct
      181 ccttctgtag tgtcagagct gttaaatatg ttcccagaat caccttttga acggttatag
      241 tcgtatttgg aaggatatcc acatgtctca gcagcatctg cacctccata attcttagca
      301 aaatcctgac gttttccttt gttaaacata aagtacttta atgcttcctg atttgctttg
      361 aaatttacaa agaaaacata tccacatgga ggatagcttc cagcaacagc tgttttgtat
      421 gtattctcaa tcggtacacg aagctgatca atctctgttg ttcccatttt tgcaacagat
      481 aggatcagtt tcttaatata agttgcgtcc atatccgtag atacatcacc aagtgcatca
      541 atcgcaatat ccacaagatc taaaggattt tgctgtaaca tcttctggaa agttgcctgc
      601 ataacagcac gctgtcttcc tgttcgtcca aactcttcaa caccatactt tttagagaac
      661 acatgacgaa ctcttgcata ccctaatgcc tgattaccat ttaaagtctg tttaccgact
      721 tttacatttc gatatttttt ctttgaaata taatttgttg tatttaagta ttctgcttct
      781 ctttgttcaa gagtcatttc aattccgccg atcctgttaa ttaccttttc aaatgcggca
      841 agatctacaa cacagaattt gtcgatctta atattaaaat tttgagccag tgtctgatat
      901 aaaagcgctt ccccgccaaa tgcataagcg gcatttagtt tattacgtcc atgacctggt
      961 atttctacat acatatctct taaaaaagaa gttaatttca attcttttgt tttaaagttg
     1021 atcgttgcaa ccatcatact gtcagatcga ccatgatcac ctttttcgga gccctgagcc
     1081 ccattatcag aaccaactaa taaaatattg acaacat
//
LOCUS       DAOMUE010000412         1108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_33906_length_1108_cov_1.306743, whole genome shotgun sequence.
ACCESSION   DAOMUE010000412 DAOMUE010000000
VERSION     DAOMUE010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33906_length_1108_cov_1.306743"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PLC71_11555"
     CDS             <1..996
                     /locus_tag="PLC71_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HRM03311.1"
                     /translation="KWKDFLIFVEQERGDIHPVVFELIGEARKMASKVNFKVKCVIAG
                     GAGTIENAHKLLDYGVDQVFAYEDESLEGFRADCYTDVVAECIAAQKPSSVLIGATAL
                     GRSLAPRLATRFHTGLTADCTTLDIKSNTDMVQIRPAFGGNIMAQISITKSRPQFATV
                     RYRVMDAAQKVESTKGEVIVCSVKEEMKQSPIEILKSEVIEKTKNIEDEDVLVVAGRG
                     VRNEKDVEMCEELAKALGGQLAFTRPMIENGFGDVAHQVGLSGRTVRPKLIITCGVSG
                     AIQFTSCMTGSECIVAINNDPEAQIFNVAHYCIVDDLYKVVPYLTELVKQQKGDE"
BASE COUNT          375 a          176 c          269 g          288 t
ORIGIN      
        1 aaatggaaag attttttgat ttttgtagaa caggaacgtg gagacattca tccggttgta
       61 tttgaattga tcggtgaagc aagaaagatg gcttcgaaag taaattttaa agtcaaatgc
      121 gtgatcgcag gtggagcagg aaccattgag aatgcacata aacttctaga ctatggagtt
      181 gatcaggtct ttgcatatga ggatgaatcc ttagaaggat tcagagcaga ctgttataca
      241 gatgttgttg cagaatgtat cgcagcacag aaaccaagtt ctgtcttaat tggggcaaca
      301 gcacttggaa gatccttagc cccaagactg gcaacaagat tccatacagg attaacagct
      361 gattgtacaa cccttgatat taagtcaaac acagacatgg tacagattcg tccagcattt
      421 ggtggaaata tcatggctca gatttctatc acaaaatcaa gaccacagtt tgcaactgta
      481 agatacagag ttatggatgc ggcacagaag gttgagagca caaaaggaga ggttattgtt
      541 tgttcagtga aggaagagat gaaacaatct ccaatcgaga tcttaaaatc cgaagtgatc
      601 gagaagacaa agaacattga agatgaagat gtcttagttg tagcaggacg aggggtccgc
      661 aatgaaaaag atgtggaaat gtgtgaagaa ttagccaaag ctttaggtgg acagttagca
      721 tttacaagac cgatgatcga gaatggtttt ggggatgttg cacatcaggt tggattatct
      781 ggaagaactg ttcgtccaaa actgatcatt acttgtggag tttctggtgc gattcagttt
      841 acatcttgta tgacaggatc tgaatgcatc gtagcgatca acaatgatcc agaagctcag
      901 attttcaatg tggcacacta ttgtatcgtt gatgatctgt acaaagtcgt tccatatctg
      961 acagaattag taaaacaaca gaaaggggac gaataagatg ccatatccat ataagaagat
     1021 gacccaggaa gattttgata agatcattga gatcacagac accgaacgtg tatgggtcgg
     1081 tgatgagatc gcaaaagaat attacaaa
//
LOCUS       DAOMUE010000413         1093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_34447_length_1093_cov_1.982659, whole genome shotgun sequence.
ACCESSION   DAOMUE010000413 DAOMUE010000000
VERSION     DAOMUE010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34447_length_1093_cov_1.982659"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1074)
                     /locus_tag="PLC71_11560"
                     /pseudo
     CDS             complement(1..1074)
                     /locus_tag="PLC71_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
BASE COUNT          278 a          265 c          181 g          369 t
ORIGIN      
        1 tgttacaagt ggtgctgttg caaagccaaa ttcttccgct ccaagcattg ctgcgatcgc
       61 cacatcacgt ccactcatta acttaccatc tgtctcaacg ataactttat tacgaagatc
      121 attcatgatc agattttgat gtgcttctgc aagtccaagc tcccaaggaa gtcctgcatt
      181 gtagatagag tttttaggag cagctcctgt accaccatca taaccagaaa tcaaaataac
      241 ctgtgctccg gctttcgcta caccagatgc aactgttccg actcctgctt cagatacaag
      301 ttttacagaa attcttgcat ctctgtttgc attcttacaa tcataaatta actgtgccaa
      361 gtcctcgata gagtaaatat catgatgagg tggtggggaa attaagctta caccggctgt
      421 tgaatgacga gttttcgcaa tccatggata aactttctta cctggaagat gtccaccttc
      481 tcctggtttt gctccctgtg ccatcttgat ctgaatctct tttgcactta ctaaatattc
      541 acttgttact ccgaatcgtc cagatgcaac ctgtttgatc gctgaacaac ggtttaatcc
      601 atctcttccg acttttaatc tctcaggtct ttctccacct tcaccactgt ttgatttacc
      661 acctaattta ttcatggcaa ttgccagtgt ctcatgtgct tctccagaaa tagatccgta
      721 agacatcgct cctgtcttga aacgtttaac gatagagtca acgctttcta cttcctcaat
      781 tggaataccc ttttctggat aatcaaattc cattaaacct cttaagtttc ctggttctaa
      841 ttccttatcg atcatatcgg tatattgttt aaacagatca taacttcctg ttcttgttgc
      901 aacctgaagt gtatggatcg tctgtggatt gtatagatgc tgttcttttc cacttctata
      961 tttgtgactt cctgcacttt ccagtgtcag atcattttct aatcccaatg gatcgaacgc
     1021 tgctccatgt cttgcatcta catctgcttc taagtctttt aaggaaatac cttcaaagac
     1081 ttagaagcag atg
//
LOCUS       DAOMUE010000414         1071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_35305_length_1071_cov_2.530512, whole genome shotgun sequence.
ACCESSION   DAOMUE010000414 DAOMUE010000000
VERSION     DAOMUE010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35305_length_1071_cov_2.530512"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..>1071
                     /locus_tag="PLC71_11565"
     CDS             162..>1071
                     /locus_tag="PLC71_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HRM03312.1"
                     /translation="MYNIVSKLLIYGDMDEESILMQLSDIFRCMDEGSQTKDVLRTRV
                     FKQVKRILEVATDYAFDKNLWHNYLTYLLITNENPFSITCEKVGASEGSVNHFAKSDF
                     KAFKELFDFDFSRIEDELGVDCFSRISDYQAIGKPELMYNKNVSEKVQALSERLETAK
                     DENEFFDMVTDFYKAYGVGMFGLNKAFRIEECGDNNIRFRAINNMDKVVLSDLVGYEI
                     QKQKLVENTEAFVEGRKANNVLLFGDSGTGKSTSIKAIVNEYYDQGLRMIEIYKHQFK
                     DLSNVIAQIKNRNYRFIIYMGDLSFEE"
BASE COUNT          409 a          166 c          213 g          283 t
ORIGIN      
        1 agatttctaa aaacataaac ttttacatga aaatctttca aaactattga aaactacaaa
       61 ccaaaaggta taaaatcgta cctaagacca aaataaacgt aacgattgct aacagttaca
      121 aaagaatgat tttaagaaaa aagtttgggg gaatacaaga gatgtataat atcgtatcaa
      181 aattactgat ctacggtgac atggacgaag aatccatctt aatgcagtta agtgatattt
      241 tccgctgcat ggacgaagga tcacagacaa aagatgtttt aagaacaaga gtatttaaac
      301 aggtaaagag aatcttagaa gttgcaacag actatgcatt tgacaagaat ctgtggcaca
      361 actatttaac atacttactg atcacaaatg aaaatccatt cagtatcaca tgcgagaaag
      421 tcggagcaag tgaaggaagt gtcaaccact ttgcaaagag tgattttaag gcatttaaag
      481 aattatttga ctttgacttt agcagaatcg aagatgaatt aggtgtagat tgcttcagca
      541 gaatctctga ttatcaggca atcggtaaac cagaattaat gtacaataag aatgtcagcg
      601 agaaagttca ggcattaagc gagagattag agacagccaa agatgagaat gaattctttg
      661 acatggttac agacttctac aaagcctatg gagttggtat gttcggtctt aacaaagcat
      721 tccgtatcga agaatgtgga gacaacaaca tccgcttccg tgcgatcaat aacatggaca
      781 aagttgtatt atccgatctt gtcggatatg agattcagaa acagaaactt gttgagaata
      841 cagaagcatt tgtagaagga agaaaagcaa acaatgttct gttatttggt gacagtggta
      901 caggtaaatc cacaagtatc aaagcgattg taaatgaata ctatgatcag ggattgcgta
      961 tgatcgagat ctacaaacat cagtttaaag atctttccaa tgtgatcgca cagatcaaga
     1021 acagaaacta cagattcatc atctacatgg gagatctttc tttcgaagaa t
//
LOCUS       DAOMUE010000415         1045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_36393_length_1045_cov_3.788889, whole genome shotgun sequence.
ACCESSION   DAOMUE010000415 DAOMUE010000000
VERSION     DAOMUE010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36393_length_1045_cov_3.788889"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /gene="nrdG"
                     /locus_tag="PLC71_11570"
     CDS             complement(<1..487)
                     /gene="nrdG"
                     /locus_tag="PLC71_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRM03313.1"
                     /translation="MRYHNITKDDMLNGDGLRAVLWVAGCTHKCKGCHNPVTWDIEGG
                     IPFDDAAKEELFEAIKPDYISGVTFSGGDPLHPANHVEVAVLAKEIKETFPNKTIWLY
                     TGFCWDEIKTLDVVKYCDVIVDGPFVQEEFDGKLHWKGSKNQRVIDVQETLKRKEIVL
                     YE"
     gene            complement(489..>1045)
                     /gene="nrdD"
                     /locus_tag="PLC71_11575"
     CDS             complement(489..>1045)
                     /gene="nrdD"
                     /locus_tag="PLC71_11575"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRM03314.1"
                     /translation="FALETLRYINEQVEEFKKEDGNLYAIYGTPAENLCGLQVKQFRK
                     KYGVIENVSDREYVSNSFHCHVTEDITPIEKQDLEGRFWELCNGGKIQYVKYPINYNK
                     EAVKTLVRRAMDKGFYEGVNLSLAYCDDCGHEELEMDVCPVCGSKNLTKIDRMNGYLS
                     YSRVKGDTRLNDAKMAEIAERKSM"
BASE COUNT          250 a          249 c          168 g          378 t
ORIGIN      
        1 attcatacaa cactatttct tttcgtttca atgtttcctg cacatcgatg actctctgat
       61 tcttacttcc cttccaatga agttttccat caaattcttc ctgtacaaaa ggtccatcta
      121 cgatcacatc gcaatatttc acaacatcca gtgtcttgat ctcatcccag cagaatcctg
      181 tatacagcca gattgtctta tttggaaatg tttctttgat ctcttttgcc aaaactgcaa
      241 cttctacatg gtttgctggg tgaagtggat ctcctccgct gaatgtgaca cctgagatat
      301 agtctggttt gatcgcttca aataattctt cttttgctgc atcatcaaat ggaattcccc
      361 cttcgatatc ccatgtgact ggattgtggc agcctttgca tttgtgggtg catcctgcaa
      421 cccataggac ggctcgcaga ccgtccccat ttagcatatc atctttcgta atattatggt
      481 aacgcatttt acatggattt cctttccgca atttctgcca ttttggcatc gttaagtctt
      541 gtatctcctt taactctgga ataagagaga tatccgttca tacggtcgat ctttgtgaga
      601 tttttacttc cgcataccgg gcacacatcc atctctaatt cttcgtgtcc acaatcatca
      661 cagtatgcta aagacagatt aactccttca tagaatcctt tatccatcgc tcttcttact
      721 aatgttttca ctgcttcttt gttatagttg atcggatatt ttacatactg gatctttcca
      781 ccattgcata attcccagaa acgtccttca agatcttgtt tctcgatcgg tgtgatgtct
      841 tctgtgacat ggcagtggaa ggaattgctc acgtattctc tgtcagatac attttcaata
      901 acaccgtatt tcttacggaa ctgttttacc tgaagaccgc ataagttttc tgctggtgtt
      961 ccatagatcg catatagatt tccgtcttcc ttcttaaatt cttctacttg ttcattaata
     1021 tagcgcaatg tttctaatgc aaact
//
LOCUS       DAOMUE010000416         1018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_37611_length_1018_cov_1.991693, whole genome shotgun sequence.
ACCESSION   DAOMUE010000416 DAOMUE010000000
VERSION     DAOMUE010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37611_length_1018_cov_1.991693"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..>1018)
                     /locus_tag="PLC71_11580"
     CDS             complement(54..>1018)
                     /locus_tag="PLC71_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRM03315.1"
                     /translation="LFMNMDSEDEGVFTVSCAGGASLICKIPYETETVNASVIEIKMT
                     GFAGGHSGVEIIKGRLNANCAMARVLLSLFNEVEMQLVSVNGGEKDNAIAKFSEASVA
                     VLPEELEAAKQIVEDTFAQIKEEYKVTDPDAKLTVNVKEAANIETFTEDTTFAVVTAM
                     VHMPNGVQRMNPEIEGLVQTSLNMGILTTEESEVQMTFAVRSSSETEKQYLIDQLTSL
                     TETLGGNVEIAGPYPGWEYKADSRLREVMVEAYKHLYNGEEPVVEGIHAGLECGIFAS
                     KLPGLDAVSFGPQMEHIHTTNEVLSISSTERTWELVVKTLAALK"
BASE COUNT          271 a          244 c          147 g          356 t
ORIGIN      
        1 tctttttata atattccacc aataatttcc gtcttcaaac tacaaatcac aatttatttc
       61 aaagcagcca acgttttcac aaccagttcc catgtacgtt ctgtagatga gatactcaac
      121 acctcatttg ttgtatgaat atgttccatc tgtggtccaa aggatactgc atcaagcccc
      181 ggcaatttag aagcaaagat tccacattca agtcctgcat gaataccttc aacaactggt
      241 tcttctccgt tgtataaatg tttatatgct tcaaccataa cctcacgtaa tctggaatct
      301 gctttgtatt cccatcctgg atatggtcct gcaatctcta cattccctcc taatgtttct
      361 gtaagagatg ttaattgatc gatcagatac tgtttttctg tctcactgga acttctaact
      421 gcaaatgtca tctgtacttc agattcttct gttgttaaaa ttcccatatt cagtgatgtc
      481 tgtaccaatc cttcaatctc tggattcatt ctctgaacac catttggcat atgaaccatt
      541 gctgttacta ctgcaaatgt tgtatcttct gtgaatgttt ctatatttgc tgcttctttt
      601 acattcactg ttaattttgc atctggatct gtgactttat attcttcttt gatctgtgca
      661 aatgtatctt caacgatctg ttttgctgct tctaattctt ctggaagtac tgcaacagat
      721 gcttcgctaa acttagcgat cgcattgtct ttttctccac catttacgga aacaagctgc
      781 atttcaacct cattaaacag acttaacaat actcttgcca ttgcacagtt tgcgttcaaa
      841 cgccctttga tgatctcgac accagaatgc ccacctgcaa atccagtcat cttgatttca
      901 atgacagatg cattgacagt ttctgtctca tatggaatct tgcagatcaa agatgctcca
      961 cctgcacagc tgactgtaaa cacgccctca tcttcagagt ccatattcat aaataaac
//
LOCUS       DAOMUE010000417         1016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_37725_length_1016_cov_1.198751, whole genome shotgun sequence.
ACCESSION   DAOMUE010000417 DAOMUE010000000
VERSION     DAOMUE010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37725_length_1016_cov_1.198751"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PLC71_11585"
     CDS             complement(<1..166)
                     /locus_tag="PLC71_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRM03316.1"
                     /translation="MENKSKGKAVERIDALLDERSFVEIGQAVTARSTDFQLDKVETP
                     SDGVVTGYGLI"
     assembly_gap    287..314
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<315..>1016)
                     /locus_tag="PLC71_11590"
     CDS             complement(<315..>1016)
                     /locus_tag="PLC71_11590"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRM03317.1"
                     /translation="ALIVNTVHLPRGKNTDVLYVSGIEDVLLTGREPLTDYSKVELNR
                     IIDTGANFTVATIRTPASVMESLSDIHLKLPIIAMDGAVLYDLKDNRYLKTYSMSYNK
                     AQKIIEILREEDVNFFINVVVEDVLIIYYHHLENEAETQIYKQMRKSPHRNYVRRNLP
                     EGEEVAYFMMVDQTEKIQKIYERLKVKIHSQEYKMLCYASKDYPGYSYIKIYDKDATR
                     ERMTKYLAEDMGIHKI"
BASE COUNT          261 a          217 c          135 g          375 t
ORIGIN      
        1 tgatcagtcc atatcctgtt acaaccccat ccgatggagt ctctactttg tctaactgaa
       61 agtctgtact tcttgctgtt accgcctgtc cgatttctac aaaacttctc tcatcgagca
      121 atgcatcaat cctctcaact gctttccctt ttgatttatt ttccataaaa agcctccttt
      181 tcttagtcat ttgtatttta ttttttccat ggtaatatga ctggttcaaa taatttcttt
      241 aattccttca ccatcgtatt cttatttgaa tcttctacca gcacatnnnn nnnnnnnnnn
      301 nnnnnnnnnn nnnnaatctt atggattccc atatcttctg ccagatattt tgtcatccgt
      361 tctctcgtag catctttatc gtaaatcttt atataagaat atcctggata atctttcgat
      421 gcataacaga gcattttata ttcctgtgaa tgtatcttaa cctttaggcg ctcatatatt
      481 ttttggattt tttccgtctg atcgaccatc ataaagtatg cgacctcttc tccttctggc
      541 agatttcttc tcacgtaatt acgatgtggt gattttcgca tttgcttgta aatctgtgtc
      601 tcagcttcat tttccaaatg atgatagtaa atgatcagta catcttctac cacaacattg
      661 ataaaaaagt ttacatcttc ttctcttaag atttctatga tcttttgagc cttattatac
      721 gacatcgaat aagtttttaa ataacgatta tccttaagat catataaaac tgctccatcc
      781 attgcgatga tcggaagttt taaatgaata tcagataatg actccatcac agatgctggt
      841 gttctgatcg ttgcaactgt aaaattggct ccagtatcaa tgatcctgtt taattctact
      901 ttgctgtaat ccgtcaacgg ctccctgcct gttaacaaga catcttcaat tcctgaaaca
      961 tacaaaacat ctgtattttt ccctcttggc aagtgaactg tattgacgat caatgc
//
LOCUS       DAOMUE010000418         1011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_37916_length_1011_cov_2.801255, whole genome shotgun sequence.
ACCESSION   DAOMUE010000418 DAOMUE010000000
VERSION     DAOMUE010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37916_length_1011_cov_2.801255"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PLC71_11595"
     CDS             complement(<1..204)
                     /locus_tag="PLC71_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRM03318.1"
                     /translation="MGKIAIVTDSNSGITQKEGKELGISVIPMPFYINEELYFEDITL
                     TQDEFYQKLNEDEDIKTSQPAPGD"
     gene            complement(329..880)
                     /locus_tag="PLC71_11600"
     CDS             complement(329..880)
                     /locus_tag="PLC71_11600"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRM03319.1"
                     /translation="MKHIILASASPRRKEILELADLKFDVMPSDAQEITTKTAPNEVV
                     MELASIKAKDIYKKSEKQSMVVGADTVVAYQGQILGKPTDEADAKRMLTMLSGQTHEV
                     YTGVCVIEDGKTKTFYEETKVTFYEISDEQIDRYIKTGEPMDKAGSYGIQGKAAVFIK
                     GIEGDYYNVVGFPIARFLQEITK"
BASE COUNT          253 a          221 c          143 g          394 t
ORIGIN      
        1 atctccaggt gcaggctgag atgttttgat atcttcatct tcgtttaact tctgataaaa
       61 ttcatcctgt gttaatgtaa tatcttcaaa atataattct tcgttaatat aaaatggcat
      121 tggaatcact gagattccta attctttacc ttctttttga gtaattccgc tgttactatc
      181 tgttacgatc gcaatcttgc ccattcttct aatccctttc tttaaatttc ctatcaatat
      241 atctattttt tgcctttatc tcacatcata acagagattc atagatatcg tcaaaggttt
      301 tcccctcata ttttgaaaaa atacataact attttgtgat ttcctgcaaa aatcttgcaa
      361 ttggaaatcc tacaacattg tagtaatctc cttcgatccc tttgataaag acagctgctt
      421 ttccttgaat cccataagat ccagccttat ccataggttc tccagtcttg atataacgat
      481 cgatctgctc atcactgatc tcataaaatg taacttttgt ttcttcataa aatgtttttg
      541 tttttccatc ttcgatgacg caaactcctg tatacacttc atgtgtctgt ccgcttaaca
      601 tcgtaagcat tctttttgcg tccgcttcgt ctgtcggttt cccaagaatc tgtccctgat
      661 atgctacaac ggtatctgct cccacgacca tactttgttt ctctgatttc ttatatatgt
      721 ctttcgcttt gatcgatgct aattccataa ctacttcatt tggtgctgtt tttgttgtga
      781 tctcttgtgc atcgcttggc ataacatcaa atttcagatc ggcaagttcc aggatttctt
      841 ttcttcttgg ggaagcagat gctaatataa tatgtttcat ttgttttata cctcttattt
      901 tacttaagtt atcaaattct cctgtgaatt acccctccca tgcggtacag tagttcctat
      961 ggaagtggct tctaacagtc gtgacagtta cccatttctg accaaaagat t
//
LOCUS       DAOMUE010000419         1008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerostipes hadrus isolate AMR_MDS_5738
            NODE_38071_length_1008_cov_2.049318, whole genome shotgun sequence.
ACCESSION   DAOMUE010000419 DAOMUE010000000
VERSION     DAOMUE010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867959
            Sequence Read Archive: SRR1616982
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerostipes hadrus (wastewater metagenome)
  ORGANISM  Anaerostipes hadrus
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Anaerostipes.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.139
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:25:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,321
            CDSs (total)                      :: 2,305
            Genes (coding)                    :: 2,261
            CDSs (with protein)               :: 2,261
            Genes (RNA)                       :: 16
            tRNAs                             :: 13
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 44
            CDSs (without protein)            :: 44
            Pseudo Genes (ambiguous residues) :: 0 of 44
            Pseudo Genes (frameshifted)       :: 29 of 44
            Pseudo Genes (incomplete)         :: 15 of 44
            Pseudo Genes (internal stop)      :: 1 of 44
            Pseudo Genes (multiple problems)  :: 1 of 44
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Anaerostipes hadrus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38071_length_1008_cov_2.049318"
                     /isolate="AMR_MDS_5738"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:649756"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-12-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..917)
                     /locus_tag="PLC71_11605"
     CDS             complement(132..917)
                     /locus_tag="PLC71_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009265391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HRM03320.1"
                     /translation="MGKVFGYPEFDENGEQILTTYDNEYKDMLMDVRVYRMKAGDEKS
                     FQKTGEETAVLLLSGSIEFAFDDVTAKADRKDVFTEGPWCVHAATESKVSVKALADSE
                     ILVQSTKNDTKFDTKLYKPEDAPWGYSSVGKFGNVAKRRVNTIFDHDIAPYSNMVLGE
                     VLNDRGNWSGYLPHRHPQPETYYFKFDRPEGFGASFVGDQVFKSTDGSFSAIPGGELH
                     PQAVAPGFQMYTCWMIRHLDGNPWLQTDRNEDERYLWLHDAQF"
BASE COUNT          254 a          241 c          155 g          358 t
ORIGIN      
        1 gtaacaatca gtgctttttt accaagctgt cttaatttgt cttctgccat atcaagtgcg
       61 ccggcacctg taaatatatt tcctggtgca ataaattctc ttgccatgtt cttttcctcc
      121 tgtcttattg attaaaattg tgcgtcatga agccaaagat atctctcatc ttcatttcga
      181 tctgtctgta accatggatt tccatccaga tggcggatca tccagcatgt atacatctgg
      241 aatcctggtg caacagcctg tggatgtaat tctccacctg ggattgcaga aaaacttcca
      301 tctgtacttt tgaatacctg atcacctacg aaacttgctc caaatccttc tggacggtcg
      361 aatttaaagt agtaagtttc tggctgtgga tgtctgtgtg gcagatatcc tgaccagttt
      421 cctctgtcat ttaatacttc tccaagtacc atattggaat atggagcaat atcatgatca
      481 aagatcgtat ttacacggcg ttttgccaca tttccaaatt tgccaacaga agaatatccc
      541 catggagcat cttctggttt atataattta gtgtcaaact ttgtatcatt ctttgttgac
      601 tgaacaagga tctcactatc tgcaagtgcc ttaacactta ccttagattc tgttgctgca
      661 tgcacacacc aaggtccttc tgtgaaaaca tcctttcgat ctgctttcgc tgtcacatcg
      721 tcaaatgcaa attcaatgga accgcttaat aacaagactg ctgtttcctc acctgtcttt
      781 tgaaaacttt tttcgtctcc tgccttcatt cgatatactc gaacatccat taacatatcc
      841 ttatattcat tgtcataagt tgttaagatc tgttctccat tttcatcaaa ttcaggatat
      901 ccaaatactt tacccattgt ctcttccttc tttctattag taagctcttg cttcttttct
      961 tccttccata acaccttcac atgccttgcg aacgctttct ttttcaga
//
